LOCUS       DAZKKW010000001        72954 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.001, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000001 DAZKKW010000000
VERSION     DAZKKW010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 72954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 72954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..72954
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.001"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..809)
                     /locus_tag="ACG8PO_000001"
     CDS             complement(<1..809)
                     /locus_tag="ACG8PO_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HFQ0205363.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAF"
     gene            complement(782..1462)
                     /locus_tag="ACG8PO_000002"
     CDS             complement(782..1462)
                     /locus_tag="ACG8PO_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFQ0205364.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(1485..2423)
                     /locus_tag="ACG8PO_000003"
     CDS             complement(1485..2423)
                     /locus_tag="ACG8PO_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HFQ0205365.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(2668..3519)
                     /gene="ispE"
                     /locus_tag="ACG8PO_000004"
     CDS             complement(2668..3519)
                     /gene="ispE"
                     /locus_tag="ACG8PO_000004"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFQ0205366.1"
                     /translation="MELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADHLFFE
                     EITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAGLGGG
                     STDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQVLPS
                     MPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMGNSLE
                     DITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGFCEEV
                     YLVRTLQ"
     gene            complement(3673..6246)
                     /gene="mprF"
                     /locus_tag="ACG8PO_000005"
     CDS             complement(3673..6246)
                     /gene="mprF"
                     /locus_tag="ACG8PO_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HFQ0205367.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            6387..7193
                     /gene="proC"
                     /locus_tag="ACG8PO_000006"
     CDS             6387..7193
                     /gene="proC"
                     /locus_tag="ACG8PO_000006"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFQ0205368.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLIDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(7272..8828)
                     /gene="rny"
                     /locus_tag="ACG8PO_000007"
     CDS             complement(7272..8828)
                     /gene="rny"
                     /locus_tag="ACG8PO_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HFQ0205369.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(9239..10288)
                     /gene="recA"
                     /locus_tag="ACG8PO_000008"
     CDS             complement(9239..10288)
                     /gene="recA"
                     /locus_tag="ACG8PO_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFQ0205370.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(10397..11638)
                     /locus_tag="ACG8PO_000009"
     CDS             complement(10397..11638)
                     /locus_tag="ACG8PO_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HFQ0205371.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(11878..12456)
                     /gene="pgsA"
                     /locus_tag="ACG8PO_000010"
     CDS             complement(11878..12456)
                     /gene="pgsA"
                     /locus_tag="ACG8PO_000010"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFQ0205372.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(12665..13588)
                     /locus_tag="ACG8PO_000011"
     CDS             complement(12665..13588)
                     /locus_tag="ACG8PO_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RodZ domain-containing protein"
                     /protein_id="HFQ0205373.1"
                     /translation="MLRRIKQTGEDERVAFETIGEKLRQARLNKKISLDELQQITKIQ
                     KRYLEAIDNDDFDQLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQ
                     PETVSTSRKAMHQEESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDT
                     ASSLKVEGSVEESSSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITD
                     ATKPVTLEFTAVNRVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNA
                     TIKANGESVPVNPGENNQSPKNVNLTLQYAE"
     gene            complement(14327..15613)
                     /locus_tag="ACG8PO_000012"
     CDS             complement(14327..15613)
                     /locus_tag="ACG8PO_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFQ0205374.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(15606..16874)
                     /locus_tag="ACG8PO_000013"
     CDS             complement(15606..16874)
                     /locus_tag="ACG8PO_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFQ0205375.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYSDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            17126..17587
                     /gene="mscL"
                     /locus_tag="ACG8PO_000014"
     CDS             17126..17587
                     /gene="mscL"
                     /locus_tag="ACG8PO_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HFQ0205376.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(17646..18092)
                     /locus_tag="ACG8PO_000015"
     CDS             complement(17646..18092)
                     /locus_tag="ACG8PO_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0205377.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGE
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(18185..18430)
                     /locus_tag="ACG8PO_000016"
     CDS             complement(18185..18430)
                     /locus_tag="ACG8PO_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HFQ0205378.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(18545..19123)
                     /locus_tag="ACG8PO_000017"
     CDS             complement(18545..19123)
                     /locus_tag="ACG8PO_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFQ0205379.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSVISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(19200..19730)
                     /locus_tag="ACG8PO_000018"
     CDS             complement(19200..19730)
                     /locus_tag="ACG8PO_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HFQ0205380.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(19734..20594)
                     /gene="rlmB"
                     /locus_tag="ACG8PO_000019"
     CDS             complement(19734..20594)
                     /gene="rlmB"
                     /locus_tag="ACG8PO_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFQ0205381.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(20587..20982)
                     /locus_tag="ACG8PO_000020"
     CDS             complement(20587..20982)
                     /locus_tag="ACG8PO_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HFQ0205382.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(20979..22391)
                     /gene="cysS"
                     /locus_tag="ACG8PO_000021"
     CDS             complement(20979..22391)
                     /gene="cysS"
                     /locus_tag="ACG8PO_000021"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFQ0205383.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(22388..22924)
                     /gene="epsC"
                     /locus_tag="ACG8PO_000022"
     CDS             complement(22388..22924)
                     /gene="epsC"
                     /locus_tag="ACG8PO_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase EpsC"
                     /protein_id="HFQ0205384.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFSLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     misc_binding    complement(23010..23249)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(23246..24706)
                     /gene="gltX"
                     /locus_tag="ACG8PO_000023"
     CDS             complement(23246..24706)
                     /gene="gltX"
                     /locus_tag="ACG8PO_000023"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFQ0205385.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(24876..26033)
                     /locus_tag="ACG8PO_000024"
     CDS             complement(24876..26033)
                     /locus_tag="ACG8PO_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HFQ0205386.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(26067..27437)
                     /gene="radA"
                     /locus_tag="ACG8PO_000025"
     CDS             complement(26067..27437)
                     /gene="radA"
                     /locus_tag="ACG8PO_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFQ0205387.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKTAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(27500..28027)
                     /locus_tag="ACG8PO_000026"
     CDS             complement(27500..28027)
                     /locus_tag="ACG8PO_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFQ0205388.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            28257..29063
                     /gene="proB"
                     /locus_tag="ACG8PO_000027"
     CDS             28257..29063
                     /gene="proB"
                     /locus_tag="ACG8PO_000027"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFQ0205389.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            29060..30307
                     /locus_tag="ACG8PO_000028"
     CDS             29060..30307
                     /locus_tag="ACG8PO_000028"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFQ0205390.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVIGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            30383..30727
                     /locus_tag="ACG8PO_000029"
     CDS             30383..30727
                     /locus_tag="ACG8PO_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0205391.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(30844..31170)
                     /locus_tag="ACG8PO_000030"
     CDS             complement(30844..31170)
                     /locus_tag="ACG8PO_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0205392.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(31785..32987)
                     /locus_tag="ACG8PO_000031"
     CDS             complement(31785..32987)
                     /locus_tag="ACG8PO_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0205393.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            33104..33619
                     /locus_tag="ACG8PO_000032"
     CDS             33104..33619
                     /locus_tag="ACG8PO_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFQ0205394.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(33818..34264)
                     /locus_tag="ACG8PO_000033"
     CDS             complement(33818..34264)
                     /locus_tag="ACG8PO_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HFQ0205395.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(34311..35789)
                     /gene="msr(C)"
                     /locus_tag="ACG8PO_000034"
     CDS             complement(34311..35789)
                     /gene="msr(C)"
                     /locus_tag="ACG8PO_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HFQ0205396.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(36143..36772)
                     /locus_tag="ACG8PO_000035"
     CDS             complement(36143..36772)
                     /locus_tag="ACG8PO_000035"
                     /EC_number="1.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFQ0205397.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            complement(36756..37220)
                     /locus_tag="ACG8PO_000036"
     CDS             complement(36756..37220)
                     /locus_tag="ACG8PO_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFQ0205398.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(37707..38975)
                     /locus_tag="ACG8PO_000037"
     CDS             complement(37707..38975)
                     /locus_tag="ACG8PO_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFQ0205399.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(39206..39943)
                     /locus_tag="ACG8PO_000038"
     CDS             complement(39206..39943)
                     /locus_tag="ACG8PO_000038"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFQ0205400.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(40010..40579)
                     /locus_tag="ACG8PO_000039"
     CDS             complement(40010..40579)
                     /locus_tag="ACG8PO_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HFQ0205401.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            40707..41579
                     /locus_tag="ACG8PO_000040"
     CDS             40707..41579
                     /locus_tag="ACG8PO_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFQ0205402.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            41921..42793
                     /locus_tag="ACG8PO_000041"
     CDS             41921..42793
                     /locus_tag="ACG8PO_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFQ0205403.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            42790..44748
                     /gene="nagE"
                     /locus_tag="ACG8PO_000042"
     CDS             42790..44748
                     /gene="nagE"
                     /locus_tag="ACG8PO_000042"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-specific PTS transporter
                     subunit IIBC"
                     /protein_id="HFQ0205404.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(45080..45931)
                     /locus_tag="ACG8PO_000043"
     CDS             complement(45080..45931)
                     /locus_tag="ACG8PO_000043"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFQ0205405.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKKLEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            46054..46521
                     /locus_tag="ACG8PO_000044"
     CDS             46054..46521
                     /locus_tag="ACG8PO_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HFQ0205406.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(46784..47266)
                     /locus_tag="ACG8PO_000045"
     CDS             complement(46784..47266)
                     /locus_tag="ACG8PO_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFQ0205407.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVIA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(47366..48229)
                     /locus_tag="ACG8PO_000046"
     CDS             complement(47366..48229)
                     /locus_tag="ACG8PO_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFQ0205408.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGARLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYELVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
     gene            48827..49702
                     /locus_tag="ACG8PO_000047"
     CDS             48827..49702
                     /locus_tag="ACG8PO_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HFQ0205409.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPQFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKTKETVKKDDFNEK"
     gene            complement(49847..49999)
                     /locus_tag="ACG8PO_000048"
                     /pseudo
     CDS             complement(49847..49999)
                     /locus_tag="ACG8PO_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
     gene            complement(50267..51598)
                     /locus_tag="ACG8PO_000049"
     CDS             complement(50267..51598)
                     /locus_tag="ACG8PO_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFQ0205410.1"
                     /translation="MGKSSRKDIVFEKFGIVATKLGNQIHLRTLRDAFATFMPFMMLA
                     GFVTLINSVILEPTGFMGEIVDTSTLTTLQQIGTSIANGTLSITTLLVVAAVSYHMCA
                     SRNYTNHIAAVLVAISTFVVLTPMQMMFTPENADKAVEVTGVIPISYTGASGMFVGIF
                     VGLAATELFIKLSNNKKLQIQLSGNIPPAVLKSFNVLIPIIITITSFALLSFIIIQLF
                     NMDINKLITTIVTEPLSKITTGLPGFLLITSIANLFFGFGIHQAVISGSLLDPFLIQN
                     MQENMAAYANKEQIPHIINMAFKDTFAVMGGSGNTIALLIAIFIFSRRKDYKDFAKLS
                     VTPAIFNISEPIIFGLPIVFNISLIIPFVLAPIFSLTVAYFATAVGLINHVVVQIPWT
                     TPPVISGFLATAGDWRAAVLQILIIAASVFIYLPFLRIDEHVTTKLSENET"
     gene            complement(51747..52460)
                     /locus_tag="ACG8PO_000050"
     CDS             complement(51747..52460)
                     /locus_tag="ACG8PO_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016611336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0205411.1"
                     /translation="MKKIAKYMEIYLDIKKKIVNGEYKIGEKLPTGDELAVIYHTSKL
                     TVKKGVDLLVSEGVLRSRSGYGTEVVRTPIDNSRVLGPNEGLFSVVGEEHVDSEIHTF
                     SIELPSKKVADKLSISVKDYVYNIVRSRFIDCQPYSIEQTFMPLAVIPGLEPKHLKKS
                     VYAYITNELGLEIKTSHIRIKGDTANEFDAKILKIDPGEFMIEIDKVVALSSGKPFEY
                     SVSRHLYQDFVFEAVFVEK"
     gene            52635..54059
                     /locus_tag="ACG8PO_000051"
     CDS             52635..54059
                     /locus_tag="ACG8PO_000051"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFQ0205412.1"
                     /translation="MTTMKKLTFPDDFWWGSAWSAEQAEGKGETGKAETIWDRWFKEQ
                     PYRFYQRIDSSITTDHIHQYKEDIELMKKTGHNSFRVSISWARMFPNGGTGKVNPKAI
                     SFYRKLFSEMNNNGIKVFANLYHFDMPATLQDKGGWESREVVDAYVHYAETCFKEFGD
                     LVCHWFTFNEPLGPILGTYLEDFHYPNLIDFKRGAQAAFFTILAHAKAIQVFKELALP
                     SKIGVILNLSPTFPRSQNPADVKAAEIADLFYTRSFLDPMVKGNFSKKLILLLQEYNQ
                     LPEDYTKEDLVCIYENTAQILGLNYYEPRRVKSRSSMVDTSSPFLPEWFFEPHIMPGR
                     RMNEYRGWEIYEKGIYDLCMDIKNHYGNIEAFISENGMGVADEERFMDDTGQIIDDYR
                     IEYIKDHLAYLWKAINEGCNIKGYHLWTFIDCWSWINAYKNRYGLVSLDLTTQKRTIK
                     KSGEFYKQLSDTNGFRYDVDLLVE"
     gene            54358..55065
                     /locus_tag="ACG8PO_000052"
     CDS             54358..55065
                     /locus_tag="ACG8PO_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HFQ0205413.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLIYAGSAQFITVSMLASHSPLLSIVLSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVILV
                     VTLIGCGLGVWIKHAFF"
     gene            55052..55375
                     /locus_tag="ACG8PO_000053"
     CDS             55052..55375
                     /locus_tag="ACG8PO_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HFQ0205414.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRILPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLVGILSLS
                     LIRLL"
     gene            complement(55464..56501)
                     /locus_tag="ACG8PO_000054"
     CDS             complement(55464..56501)
                     /locus_tag="ACG8PO_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HFQ0205415.1"
                     /translation="MSEKELMKVVGFYEGLSIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGAGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHIL
                     GKVVVSGPFNG"
     gene            complement(56822..57805)
                     /locus_tag="ACG8PO_000055"
     CDS             complement(56822..57805)
                     /locus_tag="ACG8PO_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase"
                     /protein_id="HFQ0205416.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKEPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILDTFEKEHPTLFSEQ"
     gene            complement(58220..58990)
                     /locus_tag="ACG8PO_000056"
     CDS             complement(58220..58990)
                     /locus_tag="ACG8PO_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0205417.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMIFGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(58983..59918)
                     /locus_tag="ACG8PO_000057"
     CDS             complement(58983..59918)
                     /locus_tag="ACG8PO_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0205418.1"
                     /translation="MANALEINNLKKVYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDTDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYETKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(60234..61883)
                     /locus_tag="ACG8PO_000058"
     CDS             complement(60234..61883)
                     /locus_tag="ACG8PO_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFQ0205419.1"
                     /translation="MGQREFIVLIIIAAVILLVALDIFSRLKLKETVRSKWEKIPYQP
                     RFDKEESLKEAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDQQLEKLIAFYQENPQLREKIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGLGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKLPTPLQEELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAILNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLDYPETLVETAKTEAALFDEKRQWHVLG"
     gene            complement(62000..64603)
                     /locus_tag="ACG8PO_000059"
     CDS             complement(62000..64603)
                     /locus_tag="ACG8PO_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HFQ0205420.1"
                     /translation="MVNAHIIQHTHWDREWYFTNEDAIVLSDQVFTEVLDELERNPKV
                     NFCLDGQTSIVDEYLEINPQKLKVIQRLMKTGQLFVGPWYAQTDALLVDAESILRNLM
                     IGISDTILKYGDPMMVGYLPDTFGFNANLPTLLHQVEIDNFMCWRGLNFETMVPSPYF
                     IWKGLGDKFVYAMNFPFGYMTGMMTLDAIKDIPEFAKEKLDEAVEMLHMQGKNKDILV
                     PSGVDQKSMILNFDQVVEKLNKYSKFHNIISDYPSYVEVIRNKKELPEYKGELRQPVY
                     SRVHRSIGSVRTKMKIENFKLEQKILKRVEPLMLIAERNGIHISKGLLRRLWKKILQN
                     QAHDSIGGCVSDNVAEDIFHRIKEANEIADGIENLIVKRMADALKLKSNEVLVFNTTT
                     ENYSGSKIVHVVADSKKIHFKGSPYNIIEKEVYFPERKNAYRMIATGFEYFDEPAYYE
                     LDIKINVNVPALGYTVVEFEKNNEKLETAIEIDKTEISNNKYKLSFKDGQLNLIVGDR
                     QYLDFVHLIDSANDGDTYDYSPLEGDTELSLKLETAKVYKDSLQETLVVYGKAQLPKD
                     LKDRLSEKPEMEEISYEISFSLGESQIVEGTLKIHNTVYSHRMRLAINVLDKSEKSIA
                     QIQNGFIENTPDVIPPNWSETMVEKPVNLEIFDKSVSVAENDHYFTFYADGLKEYERI
                     ENQLIITLFSTTGELGKPNLAWRPGRASGDTTNEGHVMMETPLAQEIGEYKVTFGFNV
                     EEGRFNEFKVAKQAEKRLEQSISYQKQKLNVFIHRLDNKIWPLQNELDVPRKFSLIEL
                     PEDLIVSSIYPSYFHEQGFVLRLANPTEQEKIVPEAILSLGTVVNALENKQELTTIPP
                     YDYLSILINER"
     gene            complement(64615..65664)
                     /locus_tag="ACG8PO_000060"
     CDS             complement(64615..65664)
                     /locus_tag="ACG8PO_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFQ0205421.1"
                     /translation="MEFKKQGREILNHFQSGVSYMIPLVVAAGLLTSIAVIFGGTGVW
                     DQTDTFWGVLRMIGQTGLQFIVPMISAYIAYSIADRPGLAPAFITGMMCQNLGMGFIG
                     GMVAGLACGYLANALKKVKVPMQVISLKSIMLIPFVTTLVVGIILWYVLATPIQAMTT
                     GLTNWLEGMSGANAALMSAILGGMMAFDMGGPINKIAYAFGVASFSSGSFGPSTAMLL
                     GIAIPPFGMFLATILNKKLYDESERDNAKTAMIMGLVGITEGTIPFAVKDPLRVIPSI
                     VVGTAVACGLNGFFGVTQETMLSTFMAIPFVTNIPLYLVSIVIGGVVTALMVNALKSI
                     KYKKEQQVNSVEEGK"
     gene            complement(65690..66004)
                     /locus_tag="ACG8PO_000061"
     CDS             complement(65690..66004)
                     /locus_tag="ACG8PO_000061"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HFQ0205422.1"
                     /translation="MTKIVAVTACIAGIAHTYMAKANLEKFAKKKGLDIKVETQGAMG
                     IENKLSISDVSEADVVIFAVDTNVSERGRFDGKPILTVGTSEVVKDGEETIDEAIALI
                     NK"
     gene            complement(66020..66466)
                     /locus_tag="ACG8PO_000062"
     CDS             complement(66020..66466)
                     /locus_tag="ACG8PO_000062"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose PTS transporter subunit IIA"
                     /protein_id="HFQ0205423.1"
                     /translation="MISEKININNIVIDLNASDKKSVVEKLSKLLETNGFVSDHKQFQ
                     KDVFDRENHMTTGIGKNIAIPHGKSLAVIESTVAVAKLKRSVEWGALDDQPVDLVFLL
                     AIKDTDKGTNHLRILAELSGRLMDDEFVKRIKDSRNKKELVKALSF"
     gene            complement(66467..68389)
                     /locus_tag="ACG8PO_000063"
     CDS             complement(66467..68389)
                     /locus_tag="ACG8PO_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFQ0205424.1"
                     /translation="MGIEFSERQMKILYFLIENEHTTYKELSEQLYVSTKTISKDILI
                     LKEIIQKMPDVQMLVQPKKGVSLIGKKETLRNLLSSHKINIADSPEDRLTYVFAKLLN
                     TNDSLKIQNLADELYVSRSTVESTMKDVRKRLNKHGMSLVSDRNGVRIKADEKSKRRL
                     MSEVVNYYWGGFTASNSKTDLLDLNINFIGEANNFLNKDILKKVAELLNRYMNKTQLK
                     FTDYEFQTLAIHLAIAIERIETSNFLSRSNNSEEEISKNTLILIKMIEKAFSIIVPID
                     EQAYVDIHITSIEKNAMNHDLDQTKLSYLDMGVELQELVRIKLNEFYPDEELINNLVL
                     HLNAAVKRLKLGVNIYNPYTDKIKYSFKRSFMISVDLLEEIEERFCIHFNEDEIAYVT
                     LHIQSLLDRYKPDKTKVILVCSSGYGTSKLLEQRINNGFADMVEIEDVLSINELQDRK
                     VTDELVISTIPIENTNFPVIVVSPLMLGADVRKVKQYLQLNEQTEDAFLNLLDKRFCF
                     FTHQIKDNQVNVLNIITKKLVKENYAKEGILEGAIKREKLSSTAMNDFAMPHIEVDFI
                     KKPVICVYVNPEGIDWDGTLVHSVFFFALNEKVKPELNKIYETFNEIASNHELLANLK
                     TSSSFEKLVYILKEGL"
     gene            complement(68601..69497)
                     /locus_tag="ACG8PO_000064"
     CDS             complement(68601..69497)
                     /locus_tag="ACG8PO_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFQ0205425.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWLDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDPFIRLTVANPIKPGTQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(69490..70170)
                     /locus_tag="ACG8PO_000065"
     CDS             complement(69490..70170)
                     /locus_tag="ACG8PO_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFQ0205426.1"
                     /translation="MVLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(70171..70953)
                     /locus_tag="ACG8PO_000066"
     CDS             complement(70171..70953)
                     /locus_tag="ACG8PO_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFQ0205427.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMLAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LTLGTLSVPVALTFITRSTSVSLLLSFLYIMGTAFIPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(70946..71863)
                     /locus_tag="ACG8PO_000067"
     CDS             complement(70946..71863)
                     /locus_tag="ACG8PO_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFQ0205428.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(72027..72281)
                     /locus_tag="ACG8PO_000068"
     CDS             complement(72027..72281)
                     /locus_tag="ACG8PO_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0205429.1"
                     /translation="MSTITTITMVASPLGLALFGALFDWKTTQPRLRDSLIFLGGSLA
                     IVILITGLVKLLKLDLKEAKIFSEEEYRETTLVEKKESGN"
BASE COUNT        21280 a        15347 c        12685 g        23642 t
ORIGIN      
        1 ctgaaggcaa ccaatattcg tttgatgata tttacaatca ggaacaaagc aataaagact
       61 aatgtgattg ttgctcctgg aggtgtatct agataaaacg agctagttag tcctgacagc
      121 atccctagaa atcctacaca aacactgata gcaatcacga cattgaagct tttgcctaat
      181 cgcataccga ttgctgcggg aagaaccatg attgcggata tcaaaagtgc accggcaatc
      241 gggatcatca cagcaatagc cacaccagta atcacattga acatcatcga catcaaatga
      301 acgggtagcc catccacgtg ggcggtatct tcatcaaaag tcagaacata catcggccgt
      361 ttgaataaaa agaacaatat accgatggca acgaataaga ttcctaaaaa gatgacttgc
      421 tcccatgtaa tggtcacgat cgaaccaaac agataggatt ggatacttgt tgctgaattc
      481 cctccgctca aattcatcaa tactaacgct agcgccaaac caccagccat taaaatggca
      541 atggagattt ctgaataggt tcggtagatc gttcgcagat actccaatat cacagctgct
      601 aatacaacga ctagcatggt cgtaagtgta ggattaaggt tcaaaaagaa tcctaacgct
      661 acccctgcta aagagacatg agaaagcgta tcagccatca gcgattgtct tctgatcact
      721 aaaaacacac ctagcatcgg ggcgattccg gcaatgaaga tagcggcaag aaacgctctt
      781 ctcatgaatt catatgaaag caacgccatt gagaatcctc ctttctcacc agtcggatct
      841 gtctgtccgc atactgtttg atatcttcat gatcatgggt gatcattaga actgatttcc
      901 catggacatg cgcactatgt cggagaagac gataaaattc atttctggat tcttcatcca
      961 tacctgttgt tggttcatca agaacaaata agtcagggtc agtagcaaaa atacgcgcta
     1021 aactgattcg ctgcttttgt ccgccagaca attcacctat tcgccgattt cgcatatccc
     1081 acatccctac agcttctaaa gcacgctgta catgttgatg atctttttca gagagacgct
     1141 tgaaccagcg gcctcttgga tatcttcctg aacggactaa ttcgatcacc gtactaggaa
     1201 aacctgcatt gaaagatgca acttgttgag gaatataacc gatacttaat ttttttcctt
     1261 cttgattttt ctcagcaatc tttatcattc cttttgaagg tttcagtaat cctaacgatg
     1321 ctttcaccaa ggttgacttg gctgctccat tttctccagt taaaatgaca aattcacccg
     1381 catctacatg ataagagaca tcttctagta caggttcatc atcatagtaa aaggtcaaat
     1441 cagatatttc gatataacgc accttacaac ctccattcta aaacttaatt gatactttct
     1501 tttaaagctt tcagattttt ttccatgaca gaaatataat tttcgcctgc tttgatttgt
     1561 tcattagtca agctttctaa aggattcaac acttctagct tcactcctgt ttcattcgac
     1621 aatgtctcag ctaccttcga agaggcattt tcttcaaaat aaatcacttt gatgttgttt
     1681 ttcttcacaa attcttttag ctcagctaag cgacttggtg tgggttcctg atctggtgaa
     1741 acgcccgaaa tcgctacttg ttttaagtcg tattgattcg ctaaataagc aaaagcagca
     1801 tgctgagtga caaatgtgcg gtttgttgca tcttttagct catcggagta tttttgatcc
     1861 agtgcttcaa gctttttgat ataagcatcg gcattcttcg taaaagtttc ctttttatct
     1921 ggatattttt cgcctaattc ttcagcaatc gttctgactt cttttatagc taataccgga
     1981 tcgagccaaa catgtggatc gtattcatgt gaatgaccat gcccttcttc tgattcttca
     2041 tgttcgtctg cttcttgcga ttctaaacgg tcgattcctt tagctgcttc gatcatcaag
     2101 gtctgtttgc tatccactga atcctttaac gaatccacaa aaaattccat gtctgaacta
     2161 ttatacacga aaacatctgc atcggaaatt tctgctcttt cttttgcact tggttcgaaa
     2221 tcatgcggct ccgttccagc agggatcaaa agtttcacgt ttccttcatc ccctacgatc
     2281 tcttttgtaa aatcatacat cggataaaaa gtcgtaacga tcgtcaaatc ctcttttttg
     2341 tctgcttcct tgttcgtatt tccacatgct gcaaacagta aagcacctat cattaccgta
     2401 gctgtcaaaa gatatttttt catttatatg ctcctttcaa tgaacaaatc ggaattttac
     2461 gatttattaa cgtgaactaa tgtatcaaaa taaaaaaaca cagtcaatag aaatccatca
     2521 atttctgatt taagcatgta tattcttgat taaatcgtaa aatggatgag ttgctgctca
     2581 tatctagcca tttcactaca ataaaaaact gaagaatacc attttaggat tcctcagttt
     2641 ttcatcacct attttacttc ttatatttta ttgcagggtt cgtactaaat aaacttcctc
     2701 acaaaaacct tttaacccat tatatactct ctgcgctctt gaataatgtt gacacaaggc
     2761 aaaaacggtt ggtccgcttc cactcatcaa tgcagcatct gctccgtact tgatcatacg
     2821 ctctttgatt tgatggatga ctggatgccg ctttcccgtt atatcttcta aactgtttcc
     2881 catttttttg atcatctgct gataatcttg ctctcggatg gcttcagcaa gtccgtggat
     2941 gtcttgatgt tggatggctt gcgtatctac ttctctaaac actgtacgtg tagagacact
     3001 cattcttggt ttgaccaaca caacccagca ctgtggcata gaaggtaata cttggatttc
     3061 ttcccctttg ccaccgacaa atgccgtact tccgtagaca cagtaaggaa catctgtacc
     3121 gacttccaaa ccgatttctg ccagttcttc taacgaacag cccagattcc ataattggtt
     3181 gatccctctt agagcagctg cacagtctgt acttcctccg cctaatccag ctgctactgg
     3241 aatattcttc ttaacaaaga tcttgacacc ggttttgata ttgtatttat tttttagcaa
     3301 taaagcagct tgataaacat tgttccgttc atctaccggt aaaaatgctt tatttgtttc
     3361 tacaatgatt ttgtcttctg ttatttcttc aaaaaataga tgatctgcca agtctacgct
     3421 cgacatcacc atttccaact catgataccc atcttgccgt ctatataata catccagtcc
     3481 taaatttatc ttggctggcg ctttttcaat caattccatc ctctcaccca ttcgaaaagt
     3541 ctaagtcgca agtattctat catagggaaa aataaaagta tagcttatgt ctgcctagtg
     3601 gttgattttg tcatcgtttc aacagctttc acagaatttt ctcttcataa tccaaacaag
     3661 ttctagtgat tcttataatt ctgtgagcac ttcttctgcc aaactcggaa catttccttt
     3721 ttcttttcct atcagactga gtagctgcat ggtagcaaat ccgaaattac ttcctttcct
     3781 ataagcaaaa taacgtcctt cccaatgatc gacatatttt aatttctttt cataagcaga
     3841 ctggaaggaa tagaaaaaat taccataatt atatacgata tgcatgactt tttcttgaaa
     3901 gaaagaaaaa tctgtttctc caacattagc taatggagct aagcctagat tggcttccga
     3961 gtagcccatt ttctcgtata caataaacag attggctaac agatagtcag ctaattcctc
     4021 agattcttta ggtaagaccc gcaacaaatc atatcccgcc tgcttcttcg tcatcggctg
     4081 agcggtgatg aagccaacga ctttttgatc tttacggaca ataccgacac cgctagataa
     4141 tacatatgat ttatcaaatc gtcccacaga gaagtatttt tctcgtgcac cttgaagcca
     4201 tttttgtgaa acttcattta cagcttgatg taattcttcc ggcagttgat cgtaccaagt
     4261 aaaatggtat ccttctttca tcaaacgttg atattcaagt tgctgactca gccgagacaa
     4321 gtcttctgtt gctagatcaa caatccctgc ttcacctact ttcgtgaagt gaaaacccaa
     4381 ttcatgaagc atcactacgt agctttctga tatcttataa aacgcaagct gatagcctaa
     4441 acaatctgct tgtttcataa actgattcgt tgctgcccgc atcattttag gattgccaac
     4501 tggatctcct aaaacaaagc atttgtttcc tttgatctga taaccaaaaa cgacttgatc
     4561 ttggtcgtct tcttggtaat aaaaataact gtaacctgac aagcgaagct gatggcttgt
     4621 agctgtgcct ccgtatgttt ccaataaacg gtcaaaacgg tctttttcgt actcactgcc
     4681 aagcttcgta gtatcttcag ctaaatattg ataaagcgcc actaaagcta gcaatgagat
     4741 gcccaaacca ataagccctg agaaccacac atcatctgaa ggaaacaaaa taaaatgatt
     4801 tcctgttatt cgaccgacta aaaagcgtcc gctgtgatag cctgcaattg cgtaaacgat
     4861 aaacaaaaag ccgaacaagc tagcatcaaa gattatagct ccccaagaat aaacgaattt
     4921 tgttcgataa aattcttttc tggataacca cacagctaac agaatcaaga catataccaa
     4981 tatcaactgc caagataacg tacgtgtgat tgtatttaaa ataccaaaag atagtaaagc
     5041 aatcgtaggc agaaatgcct ttttgacttt tactgaaagt gctctagcaa ggccgagtaa
     5101 taaaaagcca accaatagat tcactgtctg atctaaaaaa ttgaatgaga acggcaataa
     5161 tacttgaaat agccgactga ttgcagacag attagtgatt gttgatagaa gtaccatcat
     5221 gatccccgca aaatagacag cacttacgag cacgatatgc gcaacctttt ggaaaaacag
     5281 tcgcggaatg ttatctaaaa atcgattgat tttgactcct gtatgtgtga tgaacaatag
     5341 aagtcccgtc ataaaaggta gaacataata aaacaaccga taatataata gccaaactac
     5401 tgctacatct tgccgaacag acaattgact cattcctaag atcatcaaaa cgtcaaaagt
     5461 ccccattcca cctgggacca tcgtcagcat tcctatcaat gtagcaatca tgaacatagg
     5521 atagatcgaa agcatcgaaa tatctattct catcaacttg ccgatgatca aaaataccgc
     5581 aagggctccc gtccattgtc caaaggatgc cagtagcaga ccaagcaagc cttttggata
     5641 aaactcgaag aacaatgtct ttctctttag ataagcaaaa aagaaaatga ctggtgctaa
     5701 aaaactaccg ccgaaaagcc agatccaata gcttcgaaaa atactttctg actgaataaa
     5761 aaagacatca ataaatgtaa gaaacgacca tacagacaaa ccagtcagca taaatagtgc
     5821 gacttttgat acagtggcta aaaccatttt tctatttgtc tgctttccat aaaaactagc
     5881 ccgcagactt gctcctacca caccaccaaa accggcgaga ttgttgatcg tgtttgttac
     5941 ccacgcagca agaagaaact cttttctttc catttttggt tttccttgtt tttctaagac
     6001 tctgatcgtc acccaatcat acaacaacat cggagtgaca cccagcagtc cagccagaat
     6061 catgagcagg atcgtcatgc ggctttgttg tcccatcctt tgccaaatat cttgccaact
     6121 catgccatta gcaatatttg cgacttgatt tgccacaaag actaacacgg agccgaaaaa
     6181 aatcagtttt aaaatcagac tatgttcctt tatccattgc agcatcaagt atcgtttatt
     6241 tttcacattt tcccattcct aacttgtata cttttgtgtc ataccccaat gttatcttat
     6301 ttattttaat ttttccactt tagacctgtt ctttgcttgc gatgatagaa aaagaaagat
     6361 atcctaatgc taaaggagag gataacatga aagtaggatt tatcggcaca gggaatatgg
     6421 cacaagctat tattgatggt ttattaaaaa aaggaacagt ctcgaaagaa gaggtgttcg
     6481 tttccagtgg acattttgaa aatgcacaaa aatttgctgc tgaaactggc gtccatgcat
     6541 gcaaaagtaa tcaggaagtt gcatcacaat cagatatcgt tcttttagcc gtgaaaccaa
     6601 aaatcattga aaaagtattg gaagaactaa gagaatatag ccagaaagtc ttattcattt
     6661 ctattgcttc aggaaaatca ttaatagatt tagaaacagc gcttgaagat cctcaggcct
     6721 ccatcgtacg tgtgatgccg aatgtcaatg tcagcgtagg agcaggcgtt tctgcgattt
     6781 gcaaaaatga agctgccacg gaggaacagc ttcagcaagc tacgatgatt tttgaagcaa
     6841 tcggcagcgt ctatccttta gcagaatcag atttcagtac attcattggc cttgctggca
     6901 gctcacccgc attcgtttac atgctgatcg atgcgatggg acggactggt gtactgaatg
     6961 gtttgtccaa acagacagcc actgagattg ctgcaaaagc aattatgggt tcttgtcaaa
     7021 aattcctttc aagtgataaa aatccatggg aacttgtaga ccaagtttct tctcctggcg
     7081 gtacaactgt tgcaggtgta gttgccttag aagaagcagg attcatccca gcaatcatca
     7141 aaggaatcaa tacgacgatc gagaaagatc ttgagatgtt gaataaaaaa taatgatagc
     7201 aatgtttttt cgtgccttac aaaaaaagaa gagctgtggc ttttgccgcg gctcttcttt
     7261 tgtatttttt attatttagc ataatcagtt gcacgagttt cccgaataac gattactttg
     7321 atgtgtccag gataatccag ttcttcttcg attcgtttac gaatatcacg tactaaacga
     7381 acagcgtcta aatccgagat ttcttctggc ttgaccatta cccgaacttc gcgtccagct
     7441 tgaactgcgt aacttgattc tacaccggcg aagctgttcg aaatattttc cagacttctt
     7501 aaacgacgga tgtagttttc taatgattca cttcgcgcac caggtcttgc tgctgagagc
     7561 gcatctgctg cagctacaag gacagagata acagaagtag cttccacatc tccatgatga
     7621 gaagcgatcg cgttgattac tactgggttt tctttgtact tggcagctaa ttccgcaccg
     7681 atctcaacgt gagatccttc aatctcatgg tcaagtgctt tcccaatatc atggagcaac
     7741 ccggcacgtt ttgctaattg gatatcttcg ccaagctctg ctgctaagat accagctaat
     7801 tttgcaacct cgaccgaatg cttcaagacg ttttgtccat aactagttct gaatcgcaga
     7861 cgtccgatga tcttgattag atctggatga agtgtatgag caccgacttc aaacgctgcc
     7921 tcttccccgt attcgcgaat acgttcatcc atttctttac gagatttctc gaccatttct
     7981 tcgatacgcg ctggatggat tcgtccatct tgtatcagtt tttccaaggt catccgtgcg
     8041 atttctcttc ggattggatc gaatccggaa agaacaactg cttccggtgt atcatcgatg
     8101 attaaatcga taccagtcag cgtctcaagc gttcgaatat tacgaccttc tcgtccaatg
     8161 atacgacctt tcatttcatc attcggaagg gtaacgactg aaacagtcgt ttctgaaact
     8221 tgatctgctg cgcagcgttg aatcgctaat gataataagt ttttcgcttt gcgatctgct
     8281 tcttctttgg cacgctgttc agattctttt accattagag ttaactcatg attcagctct
     8341 tcttcagtcg atttcatgat gacatctcgt gcgtcctctt ttgataaacc agcaattttt
     8401 tctagttctt tttgttgatc ttcaatcatt ttttcaacgt ctttttctcg ctcttcaatt
     8461 aattgctgtt ttgaactaag tgcttcttct ttactttcaa gtgagttctc gcgtttttct
     8521 aacgagtcat ctttacgatt cagcgtttgt tcgcgttgaa tcaaacgatt ctcttgagat
     8581 tttaattcta acttactttc tctcagctca ctttcaactt cagaacggta tttctgattt
     8641 tcttccttag cttctaacaa tgcttctttt tttagagttt ctgcttcttt ttttgcacta
     8701 tcgatgattc cagcagctga tgattgtgca ccagctattt ctttctcgtg acgtgacttt
     8761 gcgaccataa accctaaacc aagaccgaca attaaaccga tgatagcgag gagaatgttg
     8821 aataccataa aatccacctc catactatct ttttcatttt tatcatctat acagcttcta
     8881 tgataaatta ttctaaactc gtcaatatgc atcttcgcat atatgtgtac aaaaaacatg
     8941 cacaacttta ttctaatgtt tgtaccccgt agtgtcaact ttaaataaac aaagaaacgg
     9001 ctttctactt attcattaaa cgatggtttt taaaataaat gacactagtt tcatataggt
     9061 aaacctttaa attgagaaac ttttctatta aattattctt tgtagatgaa cagatatttt
     9121 tattttatgt acccactcgt ataattaaat aactcacgat ttctttattc aataaaaaaa
     9181 cggcaggtca aggagagtac ctactcttca gaccagccgt ttttgtttat cataattttt
     9241 attcttctac taaaggcaat tcttcttgtc ctttttcatc ttctgggaca gcgacttctt
     9301 caccaatacc ataagctgct ctaactagag cagaaacttc tgccatcatt tcaggatgat
     9361 tagccatata gattttagcg ttttcccgtc cttgaccaat ccgatcttct ttataagaat
     9421 accaagcacc gcttttatca acaatgtctt tttctactgc catgtctaga agttctcctt
     9481 cttgtgagat ccctaaacca tacatgacat ctacttcagc gattttgaaa ggaggtgcta
     9541 ctttgttttt tacgactttg atctttgtac ggttaccaac aatatctgta ccttgtttca
     9601 attgttctgc acgacgtact tccaaacgga tcgttgcgta gaatttcaat gcgcgtcctc
     9661 caggagtgat ttctggattc ccaaacatca cgcctacttt ttcacggatt tggttgatga
     9721 aaatagcgat tgtttttgtt ttgttgatcg aaccagagag tttacgcaat gcttgagaca
     9781 tcaaacgtgc ttgtaacccg acatgtgagt cacccatttc gccgtcgatt tctgctcgtg
     9841 gaactaaagc agcaactgaa tcgactacta cgatatctac agccccactt gatactaaag
     9901 catcagcgat ctctagacct tgttctcctg tgtcaggctg tgaaagaagt agttcatcga
     9961 tattcacacc taatttttgt gcatattgcg gatctaacgc atgctcagca tcaatgaaag
    10021 cggccgttcc gccatttttt tgtacttctg caatagcgtg tagtgcaact gttgttttac
    10081 ctgaactttc aggtccatat acttcgatga tacgtccacg ggggtaaccg ccgacaccta
    10141 aagcgacatc taacgcaaga gagccacttg gaattgtaga gatttgttgg tcgatttttt
    10201 cgcccaattt cataatagag cctttaccat agctcttttc aatttttttc agtgcagcat
    10261 ctaaggctgc tttacgatca tctgccaata cgataatcct ccttatattt acaaatcaga
    10321 gcaacatttc attatcctta tcatatcttt tttagtgaaa aaaagcaaga aaaaaccgaa
    10381 caaatattcg catttatcat tttcttaaaa gctgttttct caaaagatca aatccttgca
    10441 taaccgcatt atatctgata gcagcacgat ctctggcaaa gtgatatttt tccgaaacca
    10501 cgccgtcttt agatgaaata gcgatccata ctgttcctgc agattgacct tctaacgtgt
    10561 ccggtccagc cactccggtg aaagctacac tgtaatctgt tcttgctatc tcacgtgctt
    10621 gaatggccat ctgctccaca cattctttgc tgactgttcc atattctgct aatagctccg
    10681 gatcaatctc cagaaaacca gcttttgttt ggagcgagta ggtaacgaat ccgcctggaa
    10741 atacttcaga tacaccagca atgtcgccta atgcggcttg gaaggcgccg gctgtcaaac
    10801 tttccgctgc tgttacagtc tgcttgtttt ctttcagtag ttccactacg acttttgcta
    10861 gcgaattatc atcgccgtat ccataaaaat attcccctac tcgttcttgg attttttctt
    10921 ctaatgctaa aagagcttga ttccctgcct gaacatcatt tgttttcact gtcagtcgta
    10981 atgtcacttc atttggtttg gcatatggtg cgatcgttgg attgatctgt gtctcaatca
    11041 gatcttttag ctcagtgacc agtcgcgatt caccaatccc ataaaagcgc aaaactttag
    11101 aaattagttt ttcttcagaa ggaaaacgtt cctctagtaa aggacgtact tgttccacaa
    11161 acattggttt caactcatta ggcggaccgg gaagaaggat atacgcatgt gtatctgttt
    11221 gataaaaagt ccctaaagca agccctgtac gatttggtag aggaaccccg ccttcgataa
    11281 tctgggactg ttgcagattg tttttggtca tttttcgatg ggttgtttca aaatatgcca
    11341 ataatttttt gtatccttcc gtattttgta tgagagactt accgaggtga gccgcagttg
    11401 cttcttttgt caggtcatct tctgtcggtc ctaatccgcc acataagagt atgagttcac
    11461 ttcttgtttc agctagagca atcaactctt ctaaacgctg ctgattatct cctacaactg
    11521 tttgataata aacttcgata cctagatcag ctagctgttc tgacaagaaa gtggcattcg
    11581 tattcacgac ttgtcctagt aaaagttctg ttccgacagc tattatttct gctttcatta
    11641 cttcccgctc ccttatatag tatacgctgt atttttatat ttttttattt aaaaagaggg
    11701 caaggagctg tgacaaaaat cttgtcacag ctccttgttc caaataaaca gtgttctcct
    11761 gccaaaacct cggtcataag tcacaatata cataaaacat gaaaagctgt tttttgaaat
    11821 tatcccttgt gactcagagt tagacggcag gctcacaacc tctccactaa acgatactta
    11881 cattgatcct ttgaacacac cgctgttttt gatgaagtaa tccacacctg aatagatagt
    11941 aaagatcaga caagcataaa gcatgatttg atctactgga ataccgatca ggctgaatgg
    12001 gatattattg ataaacagca agataatagc aaccatttgt gtagccgtct tgacttttcc
    12061 cggccaagct gcagccatga cttctccatg ttcgacaagt agtaaacgca agcctgtgac
    12121 ggctaattca cgacagacaa tgatcgctac tacccaacca ggggcctttc cttggtcaac
    12181 caacatgata aaagcagtca taactagcat tttatctgcc aacgggtccg caaacttgcc
    12241 aaaattagtg acaaggcctc gcgcacgggc aatttttcca tctaaccagt ctgtgatact
    12301 agctaaagca aaaataactg cacctactag ctgtgtgaca gccagtgtgg tgtctcctac
    12361 gttaaattgt ccccaatcta aaggtaaacc tacaaccaaa atgaaaatcg ggatcataca
    12421 aattctaagt acagttaatt tatttggtaa attcacacta tcgactcctt tcttccctat
    12481 tctacgtgac tatcagcgaa gtttccaccg atattattta cttttccaaa tgaatttatt
    12541 caaaggagta tttttattga aattgatctt tttgaattaa cgaagaagga ccgcgacaca
    12601 gatttgtcac gatccttctt ttttatacct acaagtaaac agatgcgtaa actaatttat
    12661 acgatcattc tgcgtattga agagtaaggt tcacattttt tggactctga ttgttttccc
    12721 ctggattaac tggtacggat tctccattcg ctttgatcgt tgcattgctt gctgcaccaa
    12781 tcgtgatggt cgcatttgtt gctgtttccg gtaacacggt actctgtgtt tcatttgctg
    12841 ccagtgtccc ttgataaaca taggcattgt ctactagcac acctaaccat acacgattga
    12901 cagcagtaaa ttcaagggtt acaggcttgg tagcgtctgt gatatttact gcgatggcac
    12961 tgccagaatc ttctcctgta gctattgcca ttttcttttc ttcacttgat tttgttgttg
    13021 attctactgt agaagactct ttggtctgcg tgctttgctc tgaggaagag gactcctcga
    13081 ctgatccttc tactttaagc gaagaagctg tgtctccgat tattggatca gcttgacgat
    13141 cttgccaagt catatagccc acgacaacga caatcgccaa agcaaccaat cctaataaaa
    13201 tgacaggaag acttgtccaa aagcgacttg gatgcgattc ttcctgatgc attgcttttc
    13261 tagaagtact tactgtttca ggctgcggac gttcagggac cgatcctgtc aatttgcttt
    13321 tcccatcaaa aacatcgact aaatggtctc catcttctcc tacagcttca gcgtattgcc
    13381 gaataaatgc ccgcacgtaa aatttaccag gcaattggtc aaaatcatca ttgtcgattg
    13441 cttccaaata acgtttttga atttttgtaa tctgctgtaa ttcatctaaa gatatttttt
    13501 tattcaggcg tgcttgccgt aatttttctc cgatggtttc aaaagccact cgttcatctt
    13561 ctccagtctg tttgattctc cgaagcatct gctgttgcaa cgaaagacgt tatgtttggg
    13621 tgctgtgaca aaagtcatgt cacagctcct tttttccgaa taaacattgt tctcctgcca
    13681 actcctcggt cacaagtcac aatatccaat aaatataaga agctgttttt tgaacttgtt
    13741 ccttgtgact cggagttaaa cggcaggctc acgacctctt tcttaaacgg tgttcaatca
    13801 gctgttcctc ggtattagct caaaaaccgg caaaatataa ggagatattt ttccgtatgt
    13861 ttctctaata ctcaggtcaa aacgctgatt tcacaacctc ttccttaaac ggtgttcaaa
    13921 agcttgcatc tgcggtaata agtccaacca agttaaaaac attaagaagc tgtttttact
    13981 tggctgttct tattacttga tgcagggcag ctttttcaca acctcttggt acacggtgtt
    14041 ccagaagtag cttcttcggc aataagccga aatatcacaa aaatttgaga agcaattttc
    14101 gtaaattcct tcttatttct tgaagctgac cacttctgtc acaacctctt tgattacttc
    14161 gtttgatttt attttaatat aagttgatta aaagagagca tgatttgttt tattttctca
    14221 ctaatttttc caacacaact aagaatacca taaaagctga ttttggagga ggttcctact
    14281 acaagtctaa aacgaaattt aagacttttc ctcataaaat actggtttat tttggtctca
    14341 tgtaaaactc gctgaaggcc tcttcactta taaatgcttc cccagctgcc agtacgtctt
    14401 tcatttggat cgaatcgatg atttcaggaa gatcaaataa agttcgatcg ccaaataaac
    14461 tttgcgtaaa ttgattggca atatattcga tcgaatttaa tgattggaaa tattgtccaa
    14521 gcattttctt cttcagcaaa tcgagatttg tttctgacac ttccggatca tcagcgaatc
    14581 caagaatgat ttctttgact ttttctgctg ctttctctgg ttcgtccgta tctccgctga
    14641 agtccgcaaa gtgaaattca cgatccaagc tgaattcaaa accaaagctg tcatcgataa
    14701 ttccttgatt gtacatcgct agatagtttc gagaagtatt gcctaaaatc atttggaaca
    14761 acagattgat tgctgtttta tagcgtaaca gttctgttcc ttcttgcggc aatgtgtcta
    14821 atcctttaat accaagtaca aatttatctc gtgtaatggc tgcttccaat gcactttgtt
    14881 tgatgatttc ttttgtgttt tcagggaaat aacgtacaat ttcttgcttt ggagggaaat
    14941 tttttgcttc ttggttttcc cgaataagtt tcatcaattt ctcaggctcc atcttaccaa
    15001 ctacaaataa aaccatattg cttggctgat aaaacgtccg atagcacgta tacaagtctt
    15061 gagctgtgat tttgtcgata ctttcaaccg tgcccgcaat atcgatatgc aatgggtgtg
    15121 taggatacaa gttatttagt atgccaaaaa acatccgcca gttagggtcg tcttcataca
    15181 tttggatttc ctgaccgatg attccttttt ccttgtttac agtttcttct gtgaagtaag
    15241 gtgcttgtac aaaatcgatc agagtcgtca gattcttttc tacctgatcc gtcgtagaaa
    15301 acagatagct ggttttcgta aaacttgtga aagcattggc ggaagctcct tgtttcccaa
    15361 ataattgaaa gacatcacca tcttcttttt caaacaactt atgttctaaa aaatgggcaa
    15421 ttccatctgg aactttcacc ttttcttttt caccataggg aataaattcg ttatcgatcg
    15481 aaccataatt cgtactgaac aaaccatatg tcttgtgata gtcattttta ggcaacaaat
    15541 acacggtcaa accgtttggc agtacttcat gataaagtgt ttcattgatt tgctcatatt
    15601 ctgttttatt catttgcatt tcctcctgct aaaaagaaga ttgcctgcaa ttcgatttgc
    15661 tcagctactt gttgtatctc tttgatcgtt acgctctcca tacgtttcaa ccattcttca
    15721 tcagataatt ttgtttccgg cagccagctg tccaaaaaag aagcttcgat tgccgcttgc
    15781 ggagagtcta gagaaagcag atattggttt ctcagcattg cttttgtctg agacagttcc
    15841 aactctgtta tttcaccatt tcgcaaactt tctaattgct cgtgaatcag atgaagtact
    15901 tgatttcggt tcttttcatc gataccggtt tggacactca tgaatcctcg gaacgtatcc
    15961 acgctactag aggcataata agctagactt tctttctctc tgacattcat gaaaagttta
    16021 gagtgaggaa aaccgccaaa taaaccgtta aagaccatta gcgcaaaacg ttctggttca
    16081 tcgtaataca catttgtctg ataagctagg ttcagctttg cttgaacgat tggctcacgt
    16141 acttgtcgtt ccttcacgat attcacttga ggttgggtat aaaacagatc tggataaact
    16201 gtttcccgcg actcaaatgg taattgccca actgcttcag ctgctttgtc cggatcaaca
    16261 tcgccgacaa caaaaatgtc gatttgatca tttgccatca tttcttgata gtaggacgct
    16321 agcgaactag cagtgatctt gtccaaatct tcgactacgc ccaaccctgg gatcttttgt
    16381 tccgggctgt tttcaaaata aagttcttgc aaggcatagg aagcaaaagt ttgtttatct
    16441 tccttcaaac tttccaaata tagacgcaga ttgttctttt ccaagtcaaa cgtcaactga
    16501 tcaaactgat gatttttaat atttggataa aataaaacct cttttaaaaa atctaccgct
    16561 tgagaaaaaa ggtttggatc attgacatat tcaccattca caaaactgat ccctgcgttt
    16621 agccaatgta aatttccttt ttttctaact gataatccaa agcttgcgcc ataaagctct
    16681 gcaagttgcg cacttaactt tgtctgatcc gaataattca aactatttgt ttccaacatg
    16741 ctgctgagaa gtgaacgttt cgtgatgact tcttgttgca gacgcgcagt aaaacggata
    16801 aaaatccgga tcgttttgta ttgttttgtt ggtaagacat gaagattcac gccttctgcc
    16861 aattgataca ccatatttat atgctccttt tcacagttga ttgcccgctt actcttttaa
    16921 tgggtcaacc tgcctttttt acttattcct ctgcttgaaa tttataagtt caggcaattc
    16981 gccgtttttt ctaaatgatg ttacacttta tagtatagca tagatgtttt ttatcaaaag
    17041 aaacagacgg ctaaaagcgt atattgtttt tttatttttt atttttcgat atactaaaga
    17101 caaataaaag aagggagggt cattcatgat caaagaattc aaagagttca tcatgcgtgg
    17161 agatgtcttg gatcttgctg tcggcgttgt tattggtagt gcgtttacaa gcatcgtaaa
    17221 ccaagtggtg caaggcttga tcacaccact aataggttgg tttgtcgcaa tgatcacagg
    17281 tacttctgat ttagaaggag cactttcaat tcttgactgg tcgccgacaa aaggcgtcac
    17341 atttgcgttt ggtaacgtta ttagtgctat cattacattt ctgatcactg gttttgtctt
    17401 attcttagtt gtcaaagcgg caaaccgtgc gaagaattta cgctccaaag aagagcaagt
    17461 ggaagtggaa gaaactccta ctgcagaaga ttatcttagt gacatacgcg atcttcttgc
    17521 caaacaagtg gcagaagaca atacacatcc agtagagcaa acaaatcttt ccgatacttc
    17581 taaataataa agaatctaaa aaaaagaaag ccgattttca gtgtcggctt tcttttttta
    17641 gattcttaac ttgctacttg ttcctttttc atgtcgatat gtggaatatc atcttccaaa
    17701 tacacatctg agattgcctt gaatccaaac gactcataaa aagcttgtag gtattcttgt
    17761 gcgccgatca cgattggttt ttctggaaaa tttttctgga tcatgttaat cgtttcttga
    17821 acaatctttc ttcctaaacc atctttacgg ttgtcctgat gaacaaggac acgtccaaaa
    17881 tggatactct ccgcttcttc atatactcgc gtgtaggcaa ggatcttccc atcagtatct
    17941 tgaaaaaacg tatgccaagc ttctccatca atgtcatcaa cttcttgata aggacactgt
    18001 tgttccacaa caaaaactgc gattcgttct ttgacgatcg cgtaaaactc tgctgttgac
    18061 aattcttcaa atttctttac ctgatattcc actaagcaaa cgccccctgc ttttttattc
    18121 ttagcatacc gattcgcaca caaaaaagca aacgaacctt acgaatctca ggctcgtttg
    18181 ctttttaaac tgcttcagtt aaaaattcga tctcaactgt ccgagtaagt acatctgaat
    18241 agctgtaaga tactcgttca aaagaatttt cttcaggatc caaatcaaca acgaatactg
    18301 aaggataggt ttctgttaaa acgccgcggc gttctgtttg acgctttctt cctgtctgag
    18361 caaccaacat gattgggctg ccgatacgac cttctaaatc tttcttgatt gaagctaaag
    18421 ttgttggcat acagttcacc tctactatca actataccac atatttttaa aattttcaag
    18481 tttaccataa aaaaaaaaga aatgcaataa aaataaataa gaagctcatt aagatttttc
    18541 taaattattc ctcattataa aatttcaagt gtttttttat tttgagtttt actctcccgt
    18601 aagcgctcat tacttgcttg tgatccattg ctaaacattc agccatgtct acaaaatcaa
    18661 cgccttgcat ataataatga aaaattttag cttctatttt tgaaaactgg atttcgcaat
    18721 tttcgattgc ttcttttaat aaaagatgct gaattgctaa aggttcttgc aatgtgatct
    18781 ctgctaaaaa gtctggtcct tctgtctcta ttttttggtc gattgagact tcatcttttt
    18841 gatcacgacg cttcagcgcg ttctgcttcc tcaacagaga atagatttga ttgcttataa
    18901 cgcttttata ataacttcca aagctaacac ctttgctttt ttcgtaagtt ctcaatgcct
    18961 gatataaagc gatccttgct tcttgttccc aatcctcaga aacataattt cttaaataat
    19021 aggtgttttt caaacgtagt aaaagtggaa aatatttttg atacagggca gcaaatgctt
    19081 cttcgtcacc attacgtgca gcgcgcaata actcctcaga cataaaataa ttcctccata
    19141 aattcgttta tggaggaatc ttatcatcaa tgattcatcg aactgattag aacgatatgt
    19201 tatttatttt ttctcattaa atcgtccaat ttttgggaca atttttttaa ctgctcttca
    19261 ttccatggag aattacgtcg gaaatcaata aaatgcatat ctttattttc ttgggctatt
    19321 gttttttccg ttttttttat cgtcttatat aattcataag cagaagttcg taatgctcct
    19381 tgggaaaaga tcatccactg ttctgctaaa tcgcttgtcg ccactgtcac ttgtgtcaaa
    19441 cggtcattca gctgaccagc gattctctcg atataactgt cagccgtttc gtcttctttc
    19501 gtaaaaacaa catccaactg gtatttctta taattttgtt ggatgcccgg aactagttgg
    19561 gcatcgaaga caacgatgac ttccattcct tcatatttcg cataatttga taaacgatgt
    19621 aaaagagctt ctcttgcatc cgccattttg tcctgctttt taagctggac aagttccggc
    19681 cacgaaccaa tcatgttgta gccgtctaca atcaacagct gttttttcat ggtctactcc
    19741 ccctgacgtt gggaaaaagc ctgatacata agcaaaccag cagcaacact ggcatttaag
    19801 ctctggacgt gcccggtcat cgggatcgtc agcaattcat ccatttcttt tttcaatcct
    19861 ggactcattc ctttgccttc attaccaatg actaaagcaa ttgatccttt ggcattccat
    19921 ttttggaagt ttgtgccggt catatctgtg ccaaataccc aaaatcctgt ttctttgagc
    19981 tgttgcacag cttgtaccaa attcgtcacg cgtgcaacag ggacatgttc gactgcccca
    20041 gtggaagtct ttgttacgat aggtgtgatt ccaactgctc ggtgttttgg tatgatcaca
    20101 ccatcgacgc cacttgcgtc tgctgtccgt aaaatcgacc caaaattgtg tggatcttcg
    20161 atgctatcca aaatcaaaaa gaaggggtct tcttttttct cttttgttcg ttcaatcagt
    20221 tggtcaaggg ataaatattc ataaggtgta atcgcaagaa cgactccttg atgcacgccg
    20281 tgatcgctca aagtttctaa tttttgttta ggaacccatt tgactggcac agcttgttca
    20341 cgtgcgattt ctttcagttc ttctactttt tctcctcgtg aatcttcttg aagaaagagt
    20401 ttattccctc gcccttgttg tagcgcttct actgcggcat ggtgcccaaa gacgaaatta
    20461 tcttctaatg cttcttcatt ttgatcagaa actgactgat ttcgtccagt ttgcttttta
    20521 tttgaatatc tattttgttt tttgtcttca aatccacgtt tcgattgatg tttgtttgtt
    20581 ctttctttac ccattttttt caccaacttt ctgaatacac cagcggatca attcttccat
    20641 ccgctcggtt tgttttgtca aatgcagata gcccatgagc gcttcgaacc ctgttgcgac
    20701 ccgatacgtc gtaacatcag cattttttgc gctcgtatgg cttttagcat ttcttcctct
    20761 gcgataataa agttcttctt cttctgtcaa aagatgttct tccagcattt gctgaatcaa
    20821 aaaggcttgt gcattcgcag aaacataatt cgttgccgca cgatgcagct tattgggttt
    20881 tgtttctcct tgatcaatca ggtggtcacg aacatagatt tcgtaaatcg catctccaac
    20941 ataagccaag gctaaaccgt tcaattgtgt gtaatctctc atgcttctct tctccatcgg
    21001 gttccttgcg gtgtatcttc taataagatc cctttttctt tcaacaaatc acgaatttcg
    21061 tcgcttcgga caaaatcacg attgcttctt gcttgatttc gttcttcgat caattgttca
    21121 atctcttcat ctaataactc ttctgctgta aataaaatac cgaagatcga aagccattgc
    21181 gagaataaag cttctccttg tgccaagaca gcggcagaaa ctttcggctg gtctagataa
    21241 gtgttcagcc acttagcaag ctcatagacc actgtgatcc catttgctgc attaaaatca
    21301 tcgtccattt cttcgatgaa tcgctgttct aaattttcta acaaagcgat tttatccgca
    21361 tcatctgcca aattctctga ggcagtttct tggcggaatt tcaaattgtc aaacgtattc
    21421 ttcagtcgtt gtaaattggt ggctgcttct ttcatcgtcg tttcactgta gcggatcgga
    21481 cgacggtatt gtgtggtcgc catgaagaaa cgcaagactt gtggatcaac gtttttgatc
    21541 atttcatgta cagtgatgaa attccctaga gatttactca ttttttcatc gtcttcgcca
    21601 atcgttacat agccattgtg catccagtaa ttagcgaatt ttttccctgt tttcgcttca
    21661 ctttgtgcga tttcattttc atgatgagga aattctaaat cttgcccgcc accatggata
    21721 tcgatcgttt ctcctaaatg ttttgttgcc ataacggaac attcaatatg ccaaccggga
    21781 cgtcctttgc cccatggtga atcccaagaa atctcgccag gttttgcttg tttccataaa
    21841 gcgaaatcta atgggtcttc tttttgctgc tgttcttccc ctgtacgctg acttgctccg
    21901 acttccagtt catcgatgga ttggtcactt aatttcccat aattttcaaa tttacgggta
    21961 cggtaataca catcacctga agattcataa gcatagcctt tttcaaccaa cacttgaata
    22021 aatgctaaaa tatctggcat atgatccatg actcttggat gcagtgtagc aggttcgaca
    22081 ttcaacgctt tcgtatcttc ttcaaatgct cggatgaaac gatcagcaac ttctggtgct
    22141 gtaataccta attcgtttgc tgtacggatg attttatcgt ctacatccgt gaaattcgat
    22201 acgaaatcga ctttatagcc tcgatattca aaatagcgtc gaattgtatc gaaagcaatg
    22261 gtgctacgag catttccgat atggatataa ttatacacag tcggtccgca gacatacata
    22321 ccgacatgtc ccttttgtat agggatgaat tcttcttttt ttctggtcaa tgtattgtag
    22381 atttttatca ttctgatctc ctcacttttt ctcctttgat tcgaacgact tttgctggta
    22441 ctcctaccgc tgtagcgtcg tctggaacat ctttcaacac aacggcacct gcaccgattt
    22501 tggcgttttt tccgatcgtt accggtccta atatctgcgc attcgcagag agcatggctc
    22561 cttgtttaac agttgggtgt cttttacccg tttcttttcc tgtgccgcct aatgttacgc
    22621 catggaataa aatcacatcg tcctcgattt ctgcggtttc gccaatgacg attcccattc
    22681 catggtcgat gaacacgcca gttccaatcg ttgctcctgg atggatctca acaccagtta
    22741 ggaaacgcca gaattgagca ttgatcttcg ccaatgaaaa gagccggtgc tgatataaaa
    22801 aatgagagaa acgatgccaa aacaaggcat gaaaaccagg gtatgtcaaa acgatctcag
    22861 ctgtcgaacg tgcagccgga tcgtttctct ttgctgcatc aatcgctcgg gtaaaccaac
    22921 tcatgacttt ccactccttt cgtaagaatt ttatgtagga aatcatcttc ttataaagaa
    22981 aagaagcgcc ttttaaataa gtttccttat caaaagacgc tttccgcgtg gttccacttt
    23041 tcttcataat caatgattat ctctgacagc taacgcctgt tatacgttct tggctacttt
    23101 gtttcctata aaacagtttt tcctgttttc tgtgtttcgc caagaccact cccagaggca
    23161 tttcggcaac cattcattga taccttgcac cacccggtat ctctcttgta atgatcagct
    23221 gcttactttt ttctgttcat cgtgtttagt tcagtacttg atttaaatgt gctaatgatt
    23281 tttcacgacc aagcaattcg atcgtttcag gcaattctgg accatgcatt tgtcctgata
    23341 cagctacacg gattggcatg aaaagatttt ttccttttac gccagtttct ttttgaacag
    23401 ctttgatggc agctttgaca gttggagcat ctactacttc catttgttct agctgtgctt
    23461 tgaatgcgtg gagaacagta ggaactgttt caccagcaag tacttctttt gctgcttcat
    23521 tcaatacagg atgttcattg aaaaatagcg tagacagatc aacgatttct gcagcatagc
    23581 tcatttgagg ttgatacagg ctaacgatct tcttcaccca ttcgattgtc ttttcatctg
    23641 ggttttctgc tacacgtcca tcagcgatta gataaggcag acacatgtca gtaaatgcat
    23701 ctagatccat tgctttgatg taatggttgt ttacccattg cagttttttc gcatcgaaag
    23761 ctgcgggtga tttacttagg cgttttggat cgaacatttt gatcaattct tggtgttcga
    23821 agatttcgtc ttcgcccaca ggtgaccagc ccaataatgc aataaagttg aacattgcat
    23881 ctggtagata gcctaattcg cgatattgtt cgataaattg caagattgat tcgtcacgtt
    23941 tgcttagttt tttacctgtt tcactgttga tgatcaatgt catatgtccg aaggttggtg
    24001 gtgtccagcc aaatgcttca tagatcatca gttgttttgg tgtattggca atatggtcat
    24061 ctccacgtaa aacgtgagtg attttcatca aatgatcatc gaccgcaaca gcaaaattat
    24121 aagttggcat accatcgcgt ttttggatga caaagtcgcc accgatatta tcggattcga
    24181 acgaaatagc gcctttcacc atatctgtaa atgcgtattc tgtattgcga ggcacacgga
    24241 aacgtacaac agattctaga ccttgtgctt ctttttctgc ttgttcttca ggtgtcaagt
    24301 tcgcacatgt tcctgcgtaa tgaggcattt ctcctcttgc gcgttgtgct tcccgttctg
    24361 cttctaattc ttcttctgtg caatagcatt tgtatgctcg attgctagca agcagctgat
    24421 caatcagcgg ttgatagatt tctttacgtt cagattgacg gtaagggccg tattctccag
    24481 gattttcagg agattcatcc caattcattc ccaaccaagc aaggttttcc aactggcttt
    24541 tttcgccatc ttcgatatta cgtttttggt cagtatcctc aatacgaata ataaagtctc
    24601 catcattgtg gcgggcaaac aaataattga ataatgcagt tcttgcattt ccaatatgca
    24661 ggtgcccagt tggacttggt gcatagcgta cgcgtacttt cgtcattttc ttcctcttct
    24721 ttcaaactag actttgacta acagcaaaaa ttccgtcagt tacggcttct caaatatcat
    24781 ttatttaaag ccactcagca caaattttac cttttctaag gtaaatagta aagccaactg
    24841 gttgaatttc cttaccagtt ggctgaattt cttttttatt ttgattgttt agtctttttc
    24901 tctgtctctt ttttattgtc gattcctcgt cctgcatgtg caggtttagc aaagatcatg
    24961 cgtccagcag ctgtttgaag ggcacttgtt acaacgactt cgatacgttc attcatgtaa
    25021 tgctgtccat cttctacgac aaccatcgtg ccgtcatcaa ggtaggctac tccttgttgg
    25081 cgttctgtcc cggcttttac tactagaaca ttcatcgttt caccagggat gacgactggt
    25141 tttacggcat ttgccaacgc attgatatta agaacaggta cattttggaa ttcagagact
    25201 ttattcaagt tatagtcatt ggttacaatg acaccatcta aaagcttagc caatttgatc
    25261 aatttgctgt ctacttcact gatatcttca aagtctccat cgtacatttc gacagatata
    25321 ccctcttctt tttgcaaggc atttaggata tctaatccgc gacgaccacg cacacgtttc
    25381 aaactatccc ctgagtccgc gatgtattgt agttcgtaaa gaacaaaatt agggatcatc
    25441 aaggttcctt ctaagaatcc agtctttgta atatcataaa tccgaccgtc aatgatcacg
    25501 cttgtatcca agattttata tttatggaaa ttttctcctg attttcgttc taacagttga
    25561 ctctcaactt gactagctgt ttcttgctcg atttttttgc ttcgtgaacc aaaaactttc
    25621 ttccattctt cgattcgtgt cgttcccatt ctcaatccca gatagcctaa gatgatcatc
    25681 agtaaaatcg gcaatacact attgacgaat ggaatttcca tattgtaaag aggggtcgag
    25741 atcacaacac ctaaagtcaa tccgatgatc gcaccgatcg caccgaagag aagataagtc
    25801 aagctgacct cacttagttt ttgttctact tgtttgataa tagctgagat atgtttggcc
    25861 aacagcaaag ataataaaaa gaaaataagt gcgcctaaca ggctgttggt aaatgtatta
    25921 ttcagccatc cgtttgactg ttgaccgatt gcctgccaag ctgagggtaa aaatgaaata
    25981 cccaagcttg cgccgacaat gatcatcagc aaagtaatga cacgtttttg catcgttttt
    26041 cctccgtttc gacaatcatt tattagttag cggaacacac gcttcaacgt ttcagccaaa
    26101 gttgctactc caactatctc gattcctttt ggcgcttccc agttacctaa gttgttttta
    26161 ggtaggtata cttttgtgaa gccgagtttt tggacttctt tgactcgttg ttcaatatga
    26221 ctgacacgtc tgatttctcc agtcagtcca atttcaccga tgaaacattc cgttggctgt
    26281 gttccttttt ctttatagct tgaagcaata ctgacagcga tagctaaatc aatcgctggt
    26341 tcatttaatt ttaccccacc tgcagctttt aagtaagcat cttgattctg aagaagcaac
    26401 ccagctcttt tttccagcac tgccatgatc agagataccc gattgaaatc aagacctgtt
    26461 gttgttcgtt tggcatttcc gaacatagta ggtgtgacta atgcttggat ctctaccaaa
    26521 attggacgtg tcccttccat tgccacaacg atagcagagc cagttgcacc gtctaggcgt
    26581 tcttctaaga aaatctgtga aggattagcg acttcttcca atccatgctc atgcatttca
    26641 aaaatcccaa tctcattcgt tgagccaaat cggtttttta ctgctcgcaa aatccgaaaa
    26701 ctatgatgtt gttcaccttc aaaatataac accgtatcca ccatatgttc taacattctt
    26761 gggccggcga tcgagccttc tttggtaaca tgaccgacga taaaaatggc tatcccattt
    26821 gttttagcgg ttttgagtag ttctgcagtg gtttctctta cttggctgac acttcctgcc
    26881 acacttgtga tgtctggctg cgtcatcgtt tggatggaat caatgataac ataatcaggg
    26941 gagatatgtt cgatggcacg actgatttcg ttcatatctg tttcagcgta tagataaaat
    27001 tctgtgttga tacttccgag gcgttccgcg cgtaatttta tctgctctgc gctttcttcg
    27061 ccggatacat ataagacttt tccgcctatg gctgctaatt gctgagaaac ttgtaacagc
    27121 aaagtggatt tcccgatacc tggatcgcca ccaatcaaga ccatagaccc tggcaccaca
    27181 cctccgccta acacacggtt cagttcttct aattttgttt tgatccgagg ttctttttta
    27241 gggacgacgt ctgcgatttt tgttggtttt gctttttctc ctgtcaaact tgtccgagtt
    27301 ctgcggtccg ttgtatcttg ttcgatttct tcgaccatcg tattccattt tccgcaattt
    27361 gggcagcgtc ccaagaactt aggcgacaca tacccgcaat tttggcaaac aaattgtact
    27421 tttgattttt ttgccattgt accgcctcct tttatcctaa tcatgagaca ttaaatcaag
    27481 cttaagtatc tttaacgttt tatttactgg aagaaccaaa tccgccttga cgttgagcag
    27541 tcgtttgatc ttgatctgct tttaaaaacg gcaaaaagat cccttggccg atacgctcgc
    27601 cttttttgat cactacatcc ttgaaaccaa agttggtgaa ttggaacatg atatggcctt
    27661 catttgcctc attattgtaa taatcactgt caatcacacc tactccgttt gtcaacacta
    27721 aaaaccgctt caacggattg cttgaacggt tagcaagctg caaaaattca tcttctccca
    27781 tgtaagcttt gattccagta ggcacgaggg ttggtttcaa ttgtttttca ttttctgata
    27841 catctttttt caatccatct gcgactgttt ccaacccctt tttccagcct gctttccaga
    27901 tacttggaat cacgacctct tctgctgctt caaaatcata gccagcagca tgatgtgtag
    27961 cgcgctgcgg tagattgata ttttgatctt tatatttaga aataacttca aatcctcttt
    28021 ttctcaccgg ttttcctccc taaataaact actcctattc taacatatcc gaggctgtaa
    28081 aagactttct ttacaaattc ttcaagaaat tctgctatct taaagtaaac cattaattcg
    28141 ttctttttcc aaacgactgc aagttgtaca aaaaaactat tttttacatg actttactcg
    28201 tcaaaacaag tagaatagaa cgaaattaat gagtggatat ttgattgagg tgtcacatgc
    28261 gtaaagaaat taaaaatgca aaacgaatta ttgtcaaagt aggaacaagt acattgattt
    28321 atcccaatgg aaatatcaat cttgcggcaa tcgatcagtt agcttttgtc ctttctgatc
    28381 ttagaaatcg gggaaaggaa gtgatcttag tctcatcagg agctgtaggc gttggtatgc
    28441 ataaattaca tttgtcagaa cgtccgactt tgattcctga acagcaagct gcggcggctg
    28501 ttggtcaagc cgaactgatg aatatctata atcaaagatt cagtgtatat aatcaacaga
    28561 cggcacagat cctcgtgaca agagacgtgg tagattatcc tgaaagtcgt aaaaatgtga
    28621 ggaatacatt ggaacagctt ttgattatgg acacgatccc tatcgtgaac gaaaacgata
    28681 ctgtagcagt ggatgaatta gatcatctga caaaatttgg cgataatgat cagctgtcag
    28741 ccatcatggc aaaacttgtc gcggcagacc ttttgatcgt cctttctgat atcgacgggt
    28801 tttattcaga taatccgact acaaatcccg aggctcagat gtatcgctat atcgataaaa
    28861 tcgatgaaaa gattctggca caagcaggtg gtgctggtag cacatttgga acaggcggta
    28921 tgcagagtaa attaaaagcc gcttctagga tatttgaagc aaacagcgca atgattttag
    28981 caaatggaaa aaatccggca attatttttg atattcttgc aggaaaagaa gtaggtactt
    29041 tatttaagga ggacttgaaa tgactgactt gatgcaatta ggacaacagg caaaagatag
    29101 cgcgcaacaa ctagcactta tgcctacaaa ccgcaaaaat gagctacttg ttcaaatggc
    29161 acaaaccatc aaaaagaatc aacaagcgat cattgaagca aatcataaag acttagaaaa
    29221 agcagtcgaa aacaatatca gtgaaacgat gcaagaccgc ttgcagctga cacctgagcg
    29281 aatcaatgcc atggctacag agatcgaaaa aattgcttcg ttagacgatc ctattggaaa
    29341 agtagatgag atgtggacga acaccgacgg attgagaatc ggcaaaaagc gcgtgccact
    29401 aggcgtgatc ggcatcattt acgaatctcg tccaaatgtc acgacagatg cagctagtct
    29461 agcattcaaa tcagggaatg ccgttatcct tcgcggtggc aaagaagcat tcttctcgaa
    29521 tcaattatta gtccaattat tgcagcaagt actggtcagt gctggcgaat ctccttatgc
    29581 catccagttt gtcgatgata cttctcatga aactgcagga cagttgatgc gattgacaga
    29641 atacttagat gtgctgattc ctcgaggcgg agcaaaatta atccaacgag tcaaagaaca
    29701 ggcaacggta cctatcattg aaacaggtac aggaaataat cacgtatata tcgataaaga
    29761 agcacagtta tctatggcag tgaacatcat cgtcaatgca aaagcttccc gtccatctgt
    29821 ctgtaacgcg gcagaaacat tgctgatcca ttctgagatc gcaccttttt tcttgccagc
    29881 tattgaaaaa gaactggtag aacacggggt ctctttacgt gcggatgctc gtgcgttaga
    29941 atatctagag acggctgctt tagcagaaga cacagattgg gatactgaat atctagatta
    30001 tatccttgct gtcaaagtgg tcgattcttt ggatgaagca attgcacata tcaatcggca
    30061 taatactaaa cattcagaaa cgatcgtgac agatagttat gcggctagtc aacgtttctt
    30121 aaatgaagtg gacgcggcgg ctgtctatgt caatgcctcc actcgtttta cagacggatc
    30181 ggtatttggt tttggtgcag agattggtat ctctactcaa aagttgcatg cccgtggtcc
    30241 aatggggtta aatgaattaa cttccacaaa atatatcatt tatggcgatg gacaaatccg
    30301 cgaataatgt ttcgtgtgaa acattattcc cgccttttgc cttttttctg taaaggttct
    30361 atccaactag aaaggtgtca atatgtttga aattttaatg atttttttta tttaccttat
    30421 ttctctgaat atcgctgctt ttctgggagt cggtatttta tcccttttct ttcaattcaa
    30481 aaaacgaagc atagggagtc aacgggaaaa atggtcgcag tattttgaca aaatcgggca
    30541 aaaagggctg gttacacgct tgcacattag ttacatggtt gcattatgct tattagcgac
    30601 aatcaattac tattcttttt tcgatcattc aatcgcttat acgattacgc tactgattgc
    30661 tgggattttt cacttatctt ataaatatca gctgaataaa aaccacttga atcggacttt
    30721 ccgctaaaaa atatccgtac gataagtaga gtgccttgaa gatcggcaaa aaactgccgg
    30781 actttttaga ggctgtgaca aagattttgt cacagcctct ttttccatat aaacggcgtt
    30841 cgcctaccag ctgcctggta aatctgtttc ctcttcgtta agaagcgaaa atccgtcaaa
    30901 tgtttcgcca tcataataac gaagcagctc atccatggac aagttcatcg ttttttcttt
    30961 ttcttcttcc gctgattcag ccagtagctg ttcccgatac acctgataca taccagctat
    31021 tttttgctgc atcgcttttc tagtttcacc ttccgcattg attattggca attctttgat
    31081 atagccacga taaatcaacc gatcatctga aaattcaatg ggtaattcaa cgagtgtcaa
    31141 cccgacttcc tgaaaatatt cttccagcat ctattatccc cccattcttt ttcttaaatg
    31201 aattttagct tacctactga aaattttcca gtttcattat tttgagaaaa aaagggaatg
    31261 tgacaaaaat cgtgtcacat cccctttttc cgaataaatg gtgttcttct gccaactcct
    31321 caatcataag tcacaatatt caacaaacat gagaaactgt tttttgaaat tgttccttgt
    31381 gattcagagt taaacgacag tctcacaacc tcttggtaaa cggcgttcaa tcagctgacc
    31441 ctcggtatta gctcaaaaaa cggtaaaata tgagaagcta tttttccgtt ttgtttctct
    31501 aatacttagg ataaaacgct gattttacaa cctctgttaa acggtgttca atcagctgtc
    31561 cttcggtatt agctcaaaaa acggcaaaat atgaggaact atttttccgt ttgtttctct
    31621 aatactcagg acaaaacgct gattttacaa cctcctgata aacggtgttc aaaagcttgc
    31681 atctgcggta ataagcccaa ccaaacgaaa aatatgagaa gctatttttg tttggctgtt
    31741 cttatgactt gatgcaggac agctttttca caacctctga tttcttattc ttcttcattt
    31801 gtagcaaaat aattacggtg ataaatgata caacatacac tgataagaac aatggcacct
    31861 gcaaaataaa acgcatagct ttcaccaata tgttcaccaa tcacacctgc aataaatggt
    31921 gctaaagctc caccgaaaaa gcggacaaaa ctatagctgg acgaagcaac gtttcgttgc
    31981 agacctggta tttccatagc aatcgtcgtc aacagtgtat tgataattcc ttggaaaaat
    32041 cctgctaaca caatgctgat cggtacaagg actggcatag aagttccagc tcctaataac
    32101 agcaaacaaa taatgaataa gacaaatgct gctaaaattg tccgataagt cgtaaacttc
    32161 cgctcaagag ccggtgcaac gaaaatcgaa gcaatagcca gaagcacacc ccagccaaaa
    32221 aatacaaaac cgacttctaa ttcagaatag ccaatcaata aaaatggcgc atacgcaagt
    32281 aaagtaaaga atccgaaatt atataacaat gcgattagtc caactgaacg aagtttatga
    32341 ttgcttaacg ctttgacacc ttcccaaagg cctacttttc tctttggttt ctcagtttca
    32401 ggtaaaagta ccgctagggc tataaaaccg caaaacatca aaaatgctac cccaaaaaat
    32461 ggataacgcc atgaaccact gcccaagacc ccaccgacta gagggccaac agccattccg
    32521 cagccaagtg ctgcttcata catcatgatt gctttttctg tgttaccaac taaaatactg
    32581 acgatcgttg ctaaagcagt agagacaaat aaagcattcc cgacacccca gccggcacgt
    32641 aacccaatca gcattccgat attcccagat aatccgccaa gaccagcgaa tacaacgatg
    32701 ataaacaatc ctgtcatcaa tgtttttttc aaaccgatcc gactagataa gaaacctgta
    32761 aatagcatca ccacaccagt gactaacatg taactagtaa ataacagggt cgtttcagct
    32821 ggcgtagcgt ttaactgtgc agcaatcgtt tttaagattg ggtctactag acccactccc
    32881 ataaaagcga tcatacaggt agctgctaca actagcgctg aacttttccc ctttgtttct
    32941 tcatttgttt gttcttgtat atctatttga ttctccaata tttccatccc tttctctcta
    33001 ttagcatcat acatgattta ttttacatgt caatatgcac atgtaaaacg tggaaacttt
    33061 actgtgatat aataaagaaa ataagacgag aggtaggatt tttatgaata ctttagccta
    33121 tatattatta tgcgtgcttt tatccaaacc acgttctggg tatgaattga agcagctgat
    33181 cacaattttt tgggaagccc atcacagtca gatctataca acgcttgcca agttagacca
    33241 gcagggttat gtgaccgttt tggacaaaga cgagcactct caaaaaaaga tctaccactt
    33301 gactgatgaa ggacgtgctc ttgtagagga atggatcaaa gaggaaactc ccgctcctac
    33361 gcaaaaagat gaatttctag caaaaatcta tgctttttcg acgctcgata aaacaacagc
    33421 tactggactg ctattcacta gagaacaaca gctgaacaaa atcatgcaaa aaaatcaaaa
    33481 aaaattgaat gacctctcgc tagacaagaa acaagaattt ggacgttatg ttgtgatcca
    33541 gcgcagaatt atgctttgtc agcaggaact ccaatggtgt cagcaagtaa gagaacaaat
    33601 gcagcagttt tttgagtaag ggataaacag aacctttcta gaaatatcta ttgaagagca
    33661 aattatcttc aataaaagag caatacagtt ggaaaagaaa aagcgatccg aacgacaggc
    33721 caaaacctat cgttcggatc actatttttc tagaactgca ttcaagagac tatgacaaaa
    33781 agcatgccaa ggcctttttt caaataacag tgttccatca ttcattactt tgaattgctt
    33841 ttagcacact tcttgaaagc gggccaacga tgatcaactg caacggtaaa gcggcaatca
    33901 cattcatttt ccaagcattc atataagcag agaataattg atcaggtatt ccttgttcca
    33961 tgatcacacc gaataaagac atacatgtca ccattcctaa aaccatcaag catgaaatag
    34021 ttaagatcat ttggaccttg ctttctggat ttactggtag tttaaaggca atttttttcg
    34081 ccagcacacc gatgatgaag acatccaaaa tgaaagccac cacgaatcca ggcactagac
    34141 caatcaacaa acttgataaa tgaagatctt gatgcagcca taaattatac gcgctcatcc
    34201 ccaagaccat caaaaaacac atcattgttg taaaaattat tccttctttt ttatttgtag
    34261 gcaatctgta ccactccttt aaatttgtca cgaaaatcat cgtagcacat ttaaaaattt
    34321 ctcgtaagta ctttttttat tttctgccct tttatttcat aaacttcatc agccgtacgc
    34381 tcgacaaagt aagaatcatg agaagtaaac aaaatcgttc ccggatatat ttgcattagc
    34441 ttctctaaag cttcgatcgt tgccatatca ataaaattag tcggttcatc taacagcaac
    34501 acattacttg gcttagtaaa aagcaaagca atcgtcagac gggtcgcttc tcccccactt
    34561 aatgtacata acggtttcgt caaagtttcc tgagcaaacc ctaagtgatt cagcaaacta
    34621 cggacaagcg attctgaaaa ctctgtttgt ctcatgacaa aacgaatgat ggattcttca
    34681 gatgtcattt gataaccctt ttgctgatat acttgaaagc ttactttcgg agagagaagg
    34741 attccttctc cttgtttgcg gatatgttct aataaggttg tcttacctga accattttca
    34801 ccgacaatcg cgattttttt acctaaagaa aactgaaaat caccatttac cagtaatgtt
    34861 ctcccacttc tttccaattg gacattttgt cccatgatgg gatagcggct gtggagttca
    34921 agagattttg gtactggaaa acggatttgt ttcatctgct gcggcttggt tgtttctcct
    34981 attttttgga gtcttctctc taatgttttc gcattctttt ggatggcttt ttgtacactg
    35041 tctttttgtt tagaggaaga caaccgatcc agtctgattt gttgcttttt ctttttactg
    35101 gacacttttt ccaacttttc cgcttgtttt ttcttttctt ggatcgattt ttccaatcgt
    35161 tttttctctt tcatgatcga ctctgcttct cgtagctgag tctttttctc caattccttt
    35221 tgagtgagat aggcactata attccccgaa aactctcgga tttctccgtc ttttacttcc
    35281 cagattttcg atgctagctc atctagaaaa tagcgatcat gactaacaaa aaccagcgta
    35341 ccataataat aacgaagctc ttcaatcagt cgatcaatac tttttctatc tagatgggtt
    35401 gttggctcgt ctagaagcat cccttgttca taagaagaaa gaagacgtat caactcgatc
    35461 tttcgttctt cccctccgct ccatttccgc tctgaagttt cttccagtct taactggaaa
    35521 tgactggcta attccaaatt taggtccttt gcttcagcga gataggttaa ttgaggcagg
    35581 tagttgaagg tgatctctgt ttggattttc ccatgatcgg gaaaaagttc accggctatt
    35641 agtttgagca aagtcgattt accgacacca ttttctccta tgatccctat acgatcttgc
    35701 tgatagacac gaagagaatc aatagatagt tctagctggt tgccaaagct gacttgtaga
    35761 tttgttatat ttactgctaa attttccata tgttttacac tcctatttca tgtaaaacaa
    35821 caattatcga tactaaaaaa gagcagaaaa atctgtttgc aaaaaaactg catcacatct
    35881 tttcctactc tgaacgaaat aactatgact aaatcaccaa atctgtcata gctttcacct
    35941 attctactgt cgttcgaaaa aatggacata ctccatccat gaaattgttg tttatacatg
    36001 tcttttgaat ttatttagtt gttattcaaa agttcgaaac gtaatttcat cgatgcagtc
    36061 atttttccga cctccttttt tattaggtta ccacacacga ttcaaaaaaa gcaatagcta
    36121 ctgaatagta actgaaaacc ttttattcga acatagtgat cttttctggt gctaaacgaa
    36181 cagactcgta accttcttct accgcttttc ctattgataa aataataact ggaacataac
    36241 ggtctttatc caaaccaaat gcctctgcca actgatctgc ttcgaatcca ccaattggat
    36301 ttgtttcata gccatgatca cgcgcaacta acatgaattg catagccgct aagctagaat
    36361 cgactttgac tacatcgttc atttcttctc ttgaaaaact cttgtaataa ggaatgatcg
    36421 cagctaattg ttgatctctg acttcttttg gcatcttccc tgattcgtaa gcttggttgt
    36481 agatttcttc gccatattcg taacattgca tatcgccaaa aatcatgacc attgcagaag
    36541 aagtatcatt ttgtctcgta ttgaaacgga tcaaaggacg caagatttct tttgcttcct
    36601 cgctttcagc gaccacaaag cgccatggtt tcatattgac agaagaaggt gcagtcgtcg
    36661 cttctttgat catttccagc atttcttcgt gtgggatttt gtatgtttca tcatatacgc
    36721 gaacggatct tctaccagtc ataattgttg aaaaatcatt tttttgttcc attattatta
    36781 acctctttcg tttctgttgt tatattgcaa agttgggtat tcaatttatc taaaagtgtt
    36841 agaaattgtt tcatttctac atcgctgaaa gccggggata gcacggaacc tgtatcttta
    36901 tggtccattt cacaatgctt gattttcttc tgggcttttt ctgttagcgc tacatacact
    36961 tcccggttgt tctgaggatt tctctgacgc gaaacatagc catttgcttc caataccttc
    37021 agatgacgag taactgctcc ttgatcaatc tgcaactttt cttgcaaact gctttgtagc
    37081 aatggatttt cttctttcag tctcatcatg atctcgtatc tagtaagact tataccgata
    37141 ttttcttcaa agatctggac gattttttga tcggctaatt tgatttgata taaaaggtga
    37201 ctaatctctt ctaaagacat aatttcctcc tttctaataa ttgactcgtc aacgattgac
    37261 ttgtcaataa tagaataaaa aaagaaaaaa gtcaaagaat cagctgtgta ataaaaaaaa
    37321 cgctctcaat agaaaagtat agagaaaaga aattttttgt agaatgaacg gaaaaaatga
    37381 taaagaaagc acaacgctaa aaccaagatt attgattctt atcaaactat agaagatagc
    37441 tcttctgcag tagataattt tacaccgcca aaaatttccg aaatcgcctt ttgcaagaat
    37501 cccgtcgaat attagaaatt ggatcagtat gcttaaacag taataaaaaa gttatgacaa
    37561 gatgttgtca caaccttttt tgagtaaacg gtgttcaaaa acttgcatct gcggtaataa
    37621 gttcaaccaa acaaaaaaca tgaaaatctg tttttgtttg gctgttctta ctacttgatg
    37681 cagggcagct ttttcactac ctctttttaa taaccataaa atcctattcc ttcatatctc
    37741 cagcccatcg gtgttagcca atctctttcc ttagcactca aggtatagtg atgggtacca
    37801 gttttttcat ttggattaaa tagtcgataa attggcttgg aaccaccaga ataaaaactt
    37861 agaccttcat aacgccaacc gatttttgtt aaagagttga tctcagattg tgccattgtg
    37921 taatgatggt ctccactgta aggattgtat aaacggtaga caggttgtcc gctgtttggt
    37981 gcttgccaag caatcccttc atattcccag cctacagttg gcagataatt tttttctccg
    38041 gcattcaatg tatataaatg ttctcctgta tgacggttat acagacgata catcggagaa
    38101 gttgaagttg gtgctgtatc atactgtgtc agattatagg tttcgattac attgttcaat
    38161 gtagaaccat aattaggcgc agtggcataa cgacctgtta gccaagccgt agcatctttg
    38221 taagagttcg tattgctttt ccatgcaccg gaatagtagt aaacgcctgg ctgaaaagaa
    38281 gtcgtcttca ataccgttac gttatcttgc aatgattctt tgtacgaagg atattttcta
    38341 aaagcggcat tcaccgtaat ccattgccca ttcacatatt ccgaagtagg catcgtaaca
    38401 gattggccat tatagcttcc tttaatccca aatagattgt aattaggggc agaagccaaa
    38461 gtactgttcc cccagccact ttcaacgatg gcttgagcaa tcatgactga tgcatataag
    38521 tcattggcac ttgccaaact ttgtgcagtt gaggcaatac tatcaatgaa cgcttgttga
    38581 gaattagcag atgagcgagc ataaaaagca tagccagtat agttttcgaa ttcagacaaa
    38641 tctgcttgat gggtatgtgt caatgtttgc ccatctgtca attggttcgt gatttcttct
    38701 gcattctctg gattgttctg agcttctgct gatgtactgt cttcggtttg tgtttgttct
    38761 gttgtttcag ttgttggttc ttcggttgaa attgtatttt ctgagacagt agctgactgc
    38821 aaagtacttt ctgtatcgtt ttcaattcct gtttctgaag aactcgatgt ctgagttgcg
    38881 acagtttctg tcgattcttc agctagcact gttgtgttgg caactgctag atttcctaaa
    38941 agaataacgc taaataataa attctttgct ttcatctttt ttctcctttg aattatgaaa
    39001 cttttcctaa ataacagtgt aactaaaaaa gtaaaagcaa gtcaatgtcc atatttaaat
    39061 aacatttgtt tctttttaat aaatttgctt tttctgcata aaaagacgta aatgaagaat
    39121 attcttctcc attcacgcct taaattgatg gtcgtttgaa taactattta acaagtaatt
    39181 ctctctggat attatccgtt atggattata cttcctgtgt cgtcggacgg ataatcatgt
    39241 tgctgacaga catccggtct ggcgtttcta tcgcaaatgc tacagcagaa gctatatctt
    39301 cagatgttaa tccccattct ttttgcgctt catgaagttc aagagcagcc tttttgtcgc
    39361 tgatggtttg atataattca gtttgaacag ctcctggaga gatgatcgta gatttgatgt
    39421 tattttgccg ttgctcttga cgtaatcctt ccatgattgc acggacagca aattttgttc
    39481 cgcaataaac ggcggaatca ggataaacaa catgtcccgc cacagaatca gttgaaatga
    39541 tatgcccaga tttttgtttt tccatgattg gaagtacggc ggaaattcca tttaacacac
    39601 ccatgatgtt gatatcaagc atattttgcc attcatcacg gtgaccttca actaatggag
    39661 cggttggcat gattcctgca ttattgtaca atacatcgat tcgaccgaac ttttccatcg
    39721 ctaaatcgat aactgcttgg acttgtgcaa aatctcttac atctgctgtt tgaatatata
    39781 gctcagcttc tggtaaagat tcttttattg cttttaggcg atcttctcgg cgtgcagcta
    39841 taactagttt agcccctttt tctgctaata gcttcgttgt tgcttcaccg attccgcttg
    39901 aagcgcccat gatgacaatc actttttctg ataaatcatt cattctattc tcctcctgtt
    39961 ttgaaaaatc tcttgcttat actcactatc tcttatttca tcttactgtt caaataatcc
    40021 gttcgacagg tttgacgatt ttggcaggga ttcctgcaac gatggtattg tctgggacat
    40081 ctttcgtaac agttgcattc gctgcaacaa tggcattctc gccaatcgtt acgcctggca
    40141 gaatagttgc tcctgctccg atccaagcat ttttcttgat gtggattggc tttactgaca
    40201 agcctcttcg ttcttttgga tcggttagat ggttcaccgt gatgaggttg acacgaggac
    40261 caatcaatac tgaatcttct aaacagatgc cacctaagtc aacgaacatc acttgacgat
    40321 tgataaagac atttttccct atactaatat gtttgccgaa atctgtataa aaaggcagca
    40381 gaacagtcac cgtatcatct actttgtcac taatgatatt ttctagccgt ttgcggatat
    40441 ctgctggttc atggtaccca gtattcagcg ctgctaaagg ctcttcgttt tccgcttgga
    40501 tctgatggat cacttcaaag agcggatccg ttactcgaat ctctttattg acgatttgtt
    40561 tttcgatgag gcttgtcatt ttgctccctc cttttatcct tactttataa cggacggctt
    40621 atgatgtcta atacttataa aacatactta actattcctt ttaactatac ttagtggtat
    40681 gcttttttaa aaacaggagg gaaaaaatgg atattcgtat actgacgtat tttttagcag
    40741 tagctagaga aaaaaatatc agccgagcag caaaatctct gcatatcacg caacctactc
    40801 tctcaaaaca actgaaagat ttagaagaag aacttggtgt gacacttttc actcgaggct
    40861 cccgagagat acagttgacg gattctggtc gatatcttta ttcaaaagga aaagaaatct
    40921 tgtcgttggt agaaaaaacg gcgaacaatt taacaaatga cgcaatcatc tctggagaaa
    40981 tctacattgg cggaggcgaa actaaagcaa tctctttcat ttctgatagc cttcattcgt
    41041 tgatcaaggc gcatcctgat atccagtttc atttatatag cgggaatgca gatgatatca
    41101 tagacaaact ggatagcgga ttacttgatt ttggactcgt aatcgaaccg acaaacaaac
    41161 aaaaatacca gtcatttcga ctgccagccc aagatcgttg gggacttttg atacatcgga
    41221 atcatccttt agcaaagcaa acagtcatcc atccgcagga actgaaaact gttcctttga
    41281 tcatttcccg gcaaacgtca gtggacagtc aactagctga atggctaggt ttcagcatag
    41341 atcagttatc gatcgtcgga acatataact tattgtacaa tgcttctttg atggtcgata
    41401 acgggacttt tggtgcatta gctctagatg ggatcatcaa tacttatggg acggaaaaag
    41461 tattcatccc actcgatccg ccattacaag cttcactgaa tctcatttgg aaaaaagacc
    41521 agcctctttc tcctgctgct cgtaaatttt tagatgtcct aacggagaaa tacagttaag
    41581 tagagttttt ttccttcgct ttcagctttt taacaacgaa taagttaagc aaaagatcca
    41641 atgattaaat aacataagtt tcttaatttg ttccttcctt ttatttctga taattccagt
    41701 aaattactat aattataaaa aaagaattga caaaacataa ctgtctatat aaaattgatt
    41761 acgttaacaa taattcgttt catgtgacta gatcatacta ggcatgaaaa atctctgagg
    41821 aacatggttt ccctaggagg tttttcgtgt ctatttttgt ttggccgcaa tcaactggtc
    41881 tagcaaacgt gaaaaccaaa tcttcaaaaa gggtgcgcgc atgaaaatca aaaagatctt
    41941 taatcaaaat gcagtattgg tagatgacgg tggacaggaa aaagtagcta ttggaaaagg
    42001 gattggattt gacagaaaaa gaaatgattt gatttttgat cgtgatgtcg aacggatatt
    42061 tgtcatggaa ccagaaggtc aaatcaagtt gcaaaattta cttaatcaga ttgatgaacg
    42121 ctttttattt gcagcagaaa cgatcattga tcatgcggaa actgttttga tggaaaagtt
    42181 gaatgaacat gtattgatcg ctttgaccga ccacattgct ttttctgcgg agaacattaa
    42241 taacggtatc gtcatccgca acaaactttt aagagaaatc gaagtgcttt acagtgatga
    42301 attcagtatt gctcaatggg cagtagagta tctgacaaaa gagcttggga ttccatatac
    42361 ttatgacgaa gcaggatata tcgcaatcca tattcacagc ggacgaagcg gtcggaacaa
    42421 taaccatcgc agtatccgtg aagtgacgat tgtatctgac atcatccatt tagtggaatc
    42481 agaattggaa acagatatcc attcagaacc attttcactg aattattcaa gactggtgaa
    42541 tcatttacga ctgcttctac aacgaaccca tgcgcaacaa tatgctgttt tagacacgga
    42601 aatcgtagaa atggtcaaac gaaaataccc agaaagctat aagatttcta aaaaaatccg
    42661 tgtgttgctg accaaagaat accagctcgc gatcacgaaa gaagaactgg gttacttggc
    42721 catccatatc gagcgtttaa gaagcgcaat cgtacaaaca aatgttaaca atcatgagga
    42781 ggaaaaaaat tgaaagcata tctacaacga atgggacgtt cccttcaact tccagttgcg
    42841 gtcttacctg ctgctgcttt actagtaggg atcggaaact ggtgggcatc ttatagtaac
    42901 gacattgtcg ctcatttttt acaagctggc ggtaacgcag ttttaggtca attacctttg
    42961 ctgtttgccg ttgggttagc actagggatg tcagtggaca aatcaggtgc tgctgccttg
    43021 gcaggtttgg tcgcatttga agttccagta aatgttttaa aaactgattc agtagctact
    43081 ttattgaata tcaaaccaga atcagttgat ccatcttttg ctcagatcag taacgtattt
    43141 attggtatta ttgccggatt gattgcggca gctctttata atcgtttcca cgaagcaaaa
    43201 ttaccgatgg ctttatcttt ctttagcggc aagcgtctag tgccaatctt ggctgccttt
    43261 gtcatgttgc tgatcagtgc agtgctactg ctcgtatggc ctccggttta ctctgcttta
    43321 gtttcttttg ctaaattcat tttgagttta ggctggggtg gtgcaggtct ttatggcttc
    43381 ttcaaccgtt tactgatccc tacaggattg catcaagcaa tgaactccgt gttctggttt
    43441 gacgtagcag gaatcaatga tattggtaaa tttttggctt ctgaaggaac aaaaggcgta
    43501 acaggaatgt atcaagctgg atttttccca atcatgatgt tcgggcttcc agcaggagca
    43561 tatgctattt acagaaatgc acgtccagaa aacaaagcaa aaacagcttc cttgatgtta
    43621 gcagcagctt ttgcttcatt ctttactggt gtaacagaac cacttgaatt ctcatttatg
    43681 ttcgttgctt ggccattata tgtcttgcat gcaattttca ctgggatttc tttagcagtg
    43741 agtgcattct tccattggac agcaggattt gcctttagtg ccggatttgt cgattacttc
    43801 ctatctttga aaaatccgat tgcgaatcag ccattgatgt tgatcgtcca aggattagta
    43861 acagcagtga tttactactt tggctttaac tttgctatca aaaaattcca tctgatgact
    43921 cctggccgcg aggaagcaga tctttcagat gacgatactg caacgactaa ttcagacaat
    43981 aaatatgcgg cacaagcaag caaaatctac gctgcattag gcggagccga caatgtaact
    44041 tcgatcgata actgtactac acgccttcgt ttgcaagtca aagatacagg cctgatcgat
    44101 caaaataaaa tcaaagccac tggcgtacct ggtatgaaga tcatcgacgg aaaaaatgcg
    44161 caagtcatcg taggtacaga agtacaattc gttgcagatg agatggcaaa actacatggt
    44221 ggtgctgcaa cacgacctgc acaaacaaac accgtggtta aaacagaaac atttgccggt
    44281 ggaacgatcg aacaagatat ttacgctatc gcaaatggga aactgattcc aatcactgaa
    44341 gtttcagatg atgtattcgc ggaaaaaatg atgggtgacg gctatgctgt attaccagaa
    44401 aatggagaaa tcttctctcc aatcgctggt acgatcacaa acattttccc aacgaaacat
    44461 gctgtcggaa tccaaacaga tgctggtatc gaagtcttgc ttcatatggg catcaacact
    44521 gttgacttaa aaggcgaacc attcacttta tatgtaaaag aaggtcaaaa agtcgcaaga
    44581 ggtcaattga ttgctttagt tgatcttgcc gctattcaat ctgcaggcaa aaacacggat
    44641 atggtcgttg tattcacaaa tggcgacaaa gtccaaagct tagaaatcga acccgcacgt
    44701 gatgtaaaag caaatgataa aatcggctct gtttccaaca aagcctgagc catcgtcttc
    44761 cccagacttt tttatcgata aacaaacagg agctgagaca aaaatcttgt ctcagctcct
    44821 ttttccgaat aaaaacggcg ttctcctgcc aactccgcgg taataagtcc agccaaacgt
    44881 aacatatgaa gagctatttt ttatttgact cttcttatta cttgatgcag aacagctttt
    44941 ttacaacctc tgataaacgg tgttcaaaaa ccagtttatc ggtatgaagt caaatttatg
    45001 caaaaatgtg gaaagccatt ttcacaaaat ttttcttcat actcaaaact aatcggcttt
    45061 ttctcaacct ctttatacat tattcctgat ccaatatttc ttttaatgct gcttcaagag
    45121 aagttaatgg ttttcccaat actttttcta aatcatcaga ggaaatagct aactgatcct
    45181 ttttaatatc atgttggatc gctaaaaaca tgtcagctgc ttcttgaggg aatccggctt
    45241 cttttagatt ttcgacaaac gctgcatcac ttgcttcaat aatttctaac tttttacctg
    45301 tcgcacgctc taatgccttg gctaattctt catagtcagt tagattgccg gataattcta
    45361 agatttctgg agagtcactt tctactagta cttttgctgc agcttctgca tattcacgtt
    45421 tcaatgccca acctgttttt ccatttcctg cagcataaac gaattgacca gtttttaaag
    45481 cctgcccaat gataggtaat tcattctcta cataccaatt attccgtaaa aatgtatggg
    45541 aaatacctga atccttaatc attttttcag tgaattggtg atctggcgct aatacactga
    45601 tcgagtgatc ggcatctgca aaacttgtat aagcgatata agatacccca gctgcttgtg
    45661 ctgccttcac tacattctta tgttcggctt gacgatttcc tggaacgcca gagacaaaaa
    45721 gcaatcgatc tattcctttc aacgcttctt tcattgagtc taaatcatca taatcaccaa
    45781 tacgtatatt aaatccagct tctttcagtg cttctccttt ttcttcacta cgtactagta
    45841 catagatttc attttgtgga accatctttt ttaagaattc cagcgcataa ccaccaaaac
    45901 ctccagttgc tcctgttact aaataatgca tatttgccac tccctattct ttttatttgt
    45961 taagatttac agctgtacat ttataaatta cacctttatc ataaagagtg tggtaagata
    46021 agtcaagcaa aaaaaatgaa aggatgacaa ctgatgaagt attctctgca atttagtgat
    46081 gcaatccatg tacttgccta tattgaaatg tttcaagata cccgatcact ttctagtgag
    46141 atgattgcca gtagtatcga aacaaatgca gcaaatgtac gtaaaatcat gagtcagctc
    46201 aaaaaatccg gtttgatctt tacaaaaacc ggaaaaccgc agccttccct tgccaaaagt
    46261 ccagatcaga tttctttact agatgtctat cgcagcatcg aaggtaatac acagcttatc
    46321 caagtcgatc caaaaacaaa tccaaattgc gtcgttggtg cgaatatcca agaagcttta
    46381 gaagaaagct acgctcggct gcagaaaaag gcagaagaag aaatggctaa gatcacactt
    46441 gatagtctga tacataaaat agccgttttg gaaaaagaaa aaagaccaga aaacacaggc
    46501 ctcgtagaga gatttttata attctctcta ctgtgcctgc atttttctga tcggaatcgt
    46561 ctacataaat tcttttattc tccgggggac aaaagccttg tcatgcccct ttttcctctc
    46621 taatgctcag gtcaaagcgc tgaagtcatg acgtcttgat aaacggtgtt caagtagctg
    46681 accctcggca ttagttctga aaaatgcaaa atatggaaag ctatttccca tttttcctct
    46741 ctaatgctca ggtcaaagcg ctactttcac gacctctata ttatcatggt ttattgacta
    46801 ccggtatcca gatttctccc atatatttcc cgttggtaac tcccatttcg actacagcat
    46861 ttggaccccc aacataggcg taatcttcta cttctcttaa aacatgccca aaggccgtat
    46921 tcgtcaaatc atttcttaat tcttcaggcg attctgctaa agcgattaca acaagatacg
    46981 ttccttttgg gaatgggatg attcgggtag tgtttggaac atcctgactc gtttgaacac
    47041 cgacatagta catcattttc ccgttatagg gttcgttgac tgtaaagaaa taatgatttt
    47101 cagcagcttg tttcaatgca tctattgttc cattaccgat aactttttgc tcaaatgctt
    47161 gtttctctgc agccaaaccg acaaaatccg tattgctact cttcaattcc acaccaatac
    47221 ccgtcaccgt aaaatctggt ttttcttcga tcctatactt tgccatttct cccacccttt
    47281 ctattatatt aagttcatct tacataaaat taaagggaaa tgacacgatt acgctctttt
    47341 ttctagggat atttttattt tttaactatt tttttgcttt cactgcaatc caaatctcac
    47401 tgtaaacatt actgaaatcc tctccccatt ttgtaaaaga gatctcggga gcttcgacta
    47461 actcataatc tgaagaagga agccattctg catagatttt cccccaagta tcttgtaaag
    47521 tttggggaaa tggcccttta ttaggaaaaa tggcccaagt gtgttcttct atagcaagca
    47581 tttctaaatc agcgtacgga ttttcttttg tcgtagcaaa gccaatcatg tgcatcaaac
    47641 tgcccttttc ttccaagcgg gctccatcaa atccataaga aacgttgatt acttgatttg
    47701 gatataaatc tcctaactta tgcatctctt ccctttgagt ctccgtgatc gtctgcgcta
    47761 acttttgtat ttcttgattg actccttcaa atttgacagg aacttttttc gccacacctg
    47821 ccacataaaa ctgttctttc ttttcgatac gaaattccat cgattttcct cctctgacat
    47881 caataaaaaa tgtcaagcgt ggaaaagatt tttgtatgcc gtatctgctg atttctgaag
    47941 gcagatatcc tgaccaatca cgaaaagctc ttgaaaaacc ttcgactgtt tcatatccat
    48001 atctaaacgc cgtttctgtc actcttgatc catctaggag atcgcgatta gccaaagcca
    48061 atctacggtt tttgatatag tccattagcg acatccctgc aataaacgta aagatgcgtt
    48121 ttaattgata ttcagagatt cctgtatttt cggctattat tttataggaa agtccttctg
    48181 tcaaatgttc ttcgatgtaa tcgatcaatt ggttcagatc tttaagcata tttatcttcc
    48241 tccttgcttg cctttttatc atattatcaa agaaagtcaa ttacttgatg aatcaggaac
    48301 caatttagtc agatgtagaa aatgttgtat ggatctaaag aaaattctca agcagcgttt
    48361 cgtaacggat aattggtatt atctgcctat ctatgtttta cctttagaaa aataaatttt
    48421 ggggaagaca cattctaagt aaacaaagcg gtaaaaatat cttttattcc cttagttaat
    48481 ccgcttacta cctactgtac aaagcaaatt gttgttcaaa ctgctgatca gaacgctgct
    48541 tggatttttc tttgcgatac atcatacctc ttttatcaat tggaacaatt atttatttct
    48601 aacaaaatca cttcattttt gtcagataaa taaaaaacaa aaaaagttcc atctttaaaa
    48661 cacgctattc ttttactttt tctcacattc tctaagaaaa gaaaaggaga atgaattttt
    48721 gacaattcga gcaaaagtga ttatcaaata tgtaaaatca gctacttttt tcttaaacgt
    48781 ttctttatac taaatataaa taagtgtttc atgaaaggag aactagatga ataattggct
    48841 tgagtatttt cctgaaaacg ttttggaacg cggttacagc tatcatctgc acggttttgt
    48901 ccgtcatttg aactatacaa gcaagtactt gtccgcaaca gtttctggca cggaagatta
    48961 taaggtagtg atcacttggg atgaaaagac caacatgact tgtgattgtc tatatgccat
    49021 agaaggaaaa aaatgcaaac acatggctgc tgtcttattc gcttacgaag aacgtccaat
    49081 aaaaaaatcc aactactcac tttccgaact ctcgtccctt gtttcctccg ctagttcctc
    49141 tcttgtacgg gagcttttaa cagagattct tatagaacat ccgcaattta tcgaacgctt
    49201 caaagttaag atgccatttc atgcaataaa ttattcagat aaattaacaa ctatcatcca
    49261 taaatacgat catattatca agaaaaacaa aaacaggaaa acagcgaagt tcatcatgga
    49321 gatgcgaaag ttcatccaag aagcagttga atcgctcatt cagcaaaatg cctatcttcc
    49381 cgcattcgaa ttgatcaatg aagtgattgc tactctcgaa acattctatt gggagcctga
    49441 ggatgaacga acccttcttc tgatagagga ctgttattat ttatggaaag aattattagc
    49501 agaagcacct catgcagaaa aaaggcaaat gttctcttgg ttcgtctgcc aagtcgatca
    49561 tactgacgcc tcttattcaa aacgctactc cataaaaatc ctgaaagaag actttcggga
    49621 aaaagaattt tctaaccaaa agaaaaaaat agataaaaaa actaaagaaa cggtaaagaa
    49681 agacgatttc aatgaaaaat agcaagtaaa cgataacaac catcctaaat ccacaagaaa
    49741 tacagcagac cttgctttaa aaacacgaat catgcaaagg atgaagtaaa gaagacttaa
    49801 cgcgtaccca atattagtca tttgactatt aggtacgcgt tttgttttat aagtcatgac
    49861 aaatttcctt cgttgctggg tagattttcc ggcattgttg atagatttta tgatactttt
    49921 ctacattttg taagagaggt tccactgcat ctgtatattg gataaaattc tgacaacaac
    49981 tttcaggtgt gtcgaaccac tctaatcttt aaagataaat attaaaataa cgattaataa
    50041 tattaacagt ctttattttt caccaccaaa aacctatctt tgcatatact aaatagacgt
    50101 atatacacag ataggtttgt cttgtgaaaa ataatgatta tccttaccaa aaaatgtttg
    50161 tttttctaca tgttcgtact tatcaaaaaa tatgatcctt attgaaatta ctggctattt
    50221 atattccaac gacttcttgt gttaattttt gttatctcat agtaaattat gtttcatttt
    50281 cacttaattt agttgtcacg tgttcgtcaa ttcgtaaaaa cggtaaatat ataaacacac
    50341 ttgcggctat aattaatatt tgtaaaacag ctgctcgcca gtctccggct gttgctaaaa
    50401 atcccgatat aactggtggc gtagtccaag gaatttgcac tactacatga ttaattaatc
    50461 ccacagcagt tgcaaaatat gctactgtca aagaaaaaat cggtgcaagc acaaagggaa
    50521 taatcagact gatattaaat acaataggca atccaaaaat aataggttcg cttatattga
    50581 atatagcggg agttaccgat aatttagcaa agtccttata atcttttcta cgactgaaga
    50641 taaatattgc aatcaataaa gcaatcgtat ttcccgatcc acccattacc gcaaaggtat
    50701 ctttaaaggc catattgata atatgtggaa tttgctcttt gtttgcataa gctgccatat
    50761 tttcttgcat attctgaatc aaaaacggat ctaacaatga acctgatatt actgcttgat
    50821 ggataccaaa tccaaaaaat agattagcaa tagatgtaat taacaaaaat cctggtaaac
    50881 cagttgtaat tttacttaat ggctctgtaa ctatcgtggt aattagctta tttatatcca
    50941 tattaaacaa ttgaatgatt ataaatgaca ataaagcaaa cgaggtaatc gtaataatga
    51001 ttgggattaa tacattaaaa gactttaaaa cagcgggagg aatatttccg gatagctgaa
    51061 tctgtagttt cttattatta gaaagtttaa taaataattc tgtagccgct aatccaacaa
    51121 atatacctac aaacattcct gaagctcctg tataggatat aggtatcaca cccgtcactt
    51181 caacagcttt atccgcattt tcaggagtaa acatcatctg cataggtgtt aatacaacaa
    51241 atgtcgaaat agcaactaat accgctgcaa tatgatttgt ataatttctg cttgcacaca
    51301 tatgataaga tactgctgct acaactaata atgtagttat gcttaatgta ccattggcaa
    51361 ttgaagtacc aatttgttgt aacgtggtca atgtactagt atcgacaatt tcccccatga
    51421 atcctgttgg ttctaagatc actgaattaa taagtgtcac aaatcctgct aacatcataa
    51481 atggcataaa tgtagcaaac gcatctcgca aagttcttaa atggatctga ttccccaact
    51541 tagtcgcaac tataccaaat ttttcaaata caatgtcctt cctgctactt ttgcccatat
    51601 tttttctcct ttatgcttat tgtaatcgat tactttttcc acccaaataa tacatcactt
    51661 atcaaaaaaa gtaaatactt ttaattaaat atatatatct tttttagtat aaaaaaataa
    51721 gctctgaaaa aataattttc ccgagcttat ttttcaacaa aaacagcttc aaaaacaaag
    51781 tcttgatata aatgtctcga taccgaatac tcgaaaggtt taccagacga tagagcaaca
    51841 actttgtcta tctcaatcat aaattcacct ggatctattt ttaatatttt tgcgtcaaat
    51901 tcattcgcag tatctccttt gatacgtatg tgcgaagtct ttatttccag acccagttca
    51961 tttgtgatat aagcatatac agatttcttt aaatgttttg gttccaagcc tggtatgact
    52021 gctaatggca tgaatgtttg ctcaatcgag tatggttgac aatcaataaa acggcttcgt
    52081 acaatattat atacatagtc ttttacactg atacttaatt tatcagctac ttttttcgat
    52141 ggaagttcaa ttgagaaagt atggatttct gaatctacgt gttcttcacc aaccacgcta
    52201 aacaaacctt cgtttggacc aagaactcgt gagttatcaa ttggtgttct gacgacttca
    52261 gtcccatacc cacttctact tcgcaaaact ccttcggaaa caagtaagtc tacgcctttc
    52321 tttactgtta acttgcttgt atgataaata actgccaatt catctcctgt aggaagtttc
    52381 tcaccaattt tatactctcc atttacaatt tttttcttta tatctaagta aatttccata
    52441 tacttcgcaa tttttttcaa tggtgaccct ccaatcaaaa acatatcttc atctatactt
    52501 taacataaat aaaccgataa aatccacatc aaaattatac acacaataaa aaagatatat
    52561 atatttaaac aaaactattt actttaagtt gtaagcggtg ttatcattaa gtggaaacag
    52621 gaggtaaaaa aattatgaca actatgaaaa agctaacttt tccagatgat ttttggtggg
    52681 gttccgcttg gtcagctgag caagcagaag gaaaaggaga aacaggaaaa gcagagacta
    52741 tctgggatcg ctggtttaaa gaacaacctt accggtttta tcaacgaatc gattcctcta
    52801 ttacaaccga tcacatccat caatataaag aagatataga actaatgaaa aaaactgggc
    52861 ataactcctt tcgcgtttct atttcttggg cacgaatgtt tccaaatggc ggtaccggaa
    52921 aagtaaatcc caaagccatt tctttctatc gaaaattatt ttccgaaatg aataataacg
    52981 gaatcaaagt atttgccaac ttatatcatt ttgacatgcc tgctactctt caagataaag
    53041 gaggatggga atcacgtgaa gtagttgatg cttatgttca ttacgctgaa acttgtttta
    53101 aagaatttgg tgacttagtt tgtcattggt ttaccttcaa tgaaccactt gggcctattt
    53161 taggtactta tcttgaagat tttcattatc caaacctcat tgactttaaa cgcggagcgc
    53221 aagcagcatt cttcactata cttgcacacg caaaagcaat acaagtattt aaagaactag
    53281 ctcttcctag taaaataggc gttattctaa atttgagtcc tacttttcct cgtagtcaaa
    53341 atcctgctga tgtgaaagca gcagaaatcg ctgatttatt ttatacgcgg agctttttag
    53401 atcctatggt taaagggaat ttttctaaaa agcttatcct gttattacaa gaatataatc
    53461 agcttcctga agattatacg aaagaagatc ttgtatgtat ctacgaaaat accgctcaaa
    53521 ttcttggact aaactattac gagccacgtc gcgtcaaatc tcgttcttct atggtggata
    53581 catctagtcc atttttacca gagtggtttt tcgaaccaca tatcatgcct ggacgacgaa
    53641 tgaacgaata ccggggatgg gaaatttacg aaaaaggaat ttatgatttg tgcatggata
    53701 tcaagaacca ttatggaaac atcgaagcat ttatctcgga gaacggaatg ggcgttgcgg
    53761 atgaagaacg tttcatggat gatacagggc aaataattga tgattatcgg attgagtata
    53821 tcaaagatca tcttgcttac ttatggaagg caataaatga aggatgtaac attaaggggt
    53881 atcatctttg gacatttatt gattgctggt catggatcaa cgcctataaa aaccgatatg
    53941 ggctggtttc actcgacctt actactcaaa aaagaacaat taaaaaaagt ggcgaatttt
    54001 acaagcaatt aagtgataca aatggattta gatacgatgt agacttgtta gttgaataat
    54061 attctaaaat tccacttgaa gtgaacacat tagaaaacaa gaataatgag aaataccaat
    54121 aacaaatact ttttcttcat acttcattta tacttgtcag tataagagac tgtgacaaat
    54181 gtagtgatcc tcagaagtta ggtctggaaa tctaacttga gaagattttt ttaaactacg
    54241 tccagtcttt tttatttgtt taaaattgaa gttgacaagt cttcattaaa aatttagaat
    54301 attcacaagt ttgacgacaa ttatgaaaat ttatgtcgtg ctaaaggagt tttgttattg
    54361 gatgaaaagt tagatataaa aactgcgatc aaagacacgt tacctaccgt ttttggttat
    54421 atcggtattg gacttgcatt tggtatcgtt gggaaagctg ccgggtttca tccgctggtc
    54481 gtcacgttga tgtccctgct gatctatgct ggttctgccc aatttatcac ggtcagcatg
    54541 cttgctagcc atagtccact gctctccatc gttctctcga cctttctagt caattcccga
    54601 atgattctga tgagtatgac gattgctcct tatttcaaga aaaatcggct gcttcaaaat
    54661 ctgctgattg gcacattgct gacagatgaa agtttcgcac tgggaatgaa taaattgaac
    54721 tatactggac aaaagttaaa ctttcgttgg atgaatacag ctaattggat ttcttatttg
    54781 acgtgggtgg gttcttctct tgttggcgca cttttaggga attttattac tgatccgaaa
    54841 aagtttggtt tggatttcgc catcgtcgct atgtttatcg ggttacttta tcttcaagtc
    54901 atttctgata gaaacacctc taaacgatta cagttgatcc tgattggtct cactttgatt
    54961 ctggtatatg tcggactgat ttttatccca agtaacttag tgattttagt tgtaacactg
    55021 attgggtgcg gattgggggt gtggatcaaa catgccttct tttgagttta cattgctgac
    55081 tattttaggt tgtactttag caacttggct ttctaggatc ctaccatttg tcttactgaa
    55141 aaaattcgat ttgccgcaac cgttgcttga gtatttaagt tttgtcccga tcgtcattat
    55201 gtctgcttta tggttcagca gtctatttac ccaaaatatc ggacatctgc cgcagatcaa
    55261 tctcgaaaat gctttggctt cagttcctac acttttagct gctattcttt caaaaagctt
    55321 attggtgatc gtcttggtag gaattctttc cctttcgttg attcgcttat tataaataaa
    55381 aaaacaagag cttatggcaa attactgtca tgcgctcttg tttttatcaa agtcaaataa
    55441 gaaaaaaatc ggcgtattct tgcttatccg ttgaacggac cactaacaac gacttttcct
    55501 aatatatgtc ctgtttctac gtctttggtt gcttgtttca agttcttcgc attcatccca
    55561 tctgaataga ccttgctcaa tgtactgcgg ataatccctt catttgctaa agcagcaatg
    55621 tgcgctaaag cttctccttg cgtttcgatt tcatagttat agtctgtttt agcgaacata
    55681 tactcccagt cgaagctgat agattggttt ttccaaacac ctatatctaa cggctctttg
    55741 atccctacga tcgttcccac atgtccaaat ggtcggacaa ggtgtttgat ttgatcaaag
    55801 tagcgggtaa tatcgaataa tacagcgaca tactctactt gatcgattcc taattcacga
    55861 aaacttttac ttaagtcgtg atgataatcg attggatagt ctacacctgt ttttttcagc
    55921 cattcaaaat tcttcggact tgcagtcgcg tagacttcta ggccagccca atgcgctagt
    55981 tgattcaaaa tcgaaccgac gcccccagct ccgttgatca ctaagatttt tttcccgcgg
    56041 ttggcatttt cttctggagt cagaccaaat ttttcaaata acaattcata agcggtcaat
    56101 gaggtcaacg gcaaggctgc ggcttcttca tctgaaagat tttttggtgc aagagcgaca
    56161 atacgttcgt caaccagttg gtattcttga ttgcttcctg ctcttgtcgt tgtacctgcg
    56221 taaaaaacgc gatcgccgac agaaaatttt tgcacttcat cacctacagc aacaactttc
    56281 ccgactccat caaaccctaa gatacgtaaa gcatttctta tcccgttatc ttggcgaagt
    56341 ttcgtatcga caggattaac agatactgcg ttgactttta ccagaagatc ccgtgaactg
    56401 ggtacaggta tctgttcttt ttcatcaata aagctttttg gatcatcgat cgacaaccct
    56461 tcataaaaac cgactacctt cattaattcc ttttctgaca tacgaattcc tccttagctt
    56521 catcaagttg gagcatccat acagttttca tttcaatata tatagtatgc tatttgttcc
    56581 acttttattc atcataatta ttctcttact taaagtaaac tatttgcact tttttggtta
    56641 aaaaagaggt tgcgacaaga cttttgtcac aacctctttt aaacggtgtt caatcaactg
    56701 accctcggta ttcgctcaaa aaacgacaaa atatgaggag ctatttttgt ttggctgttc
    56761 ttattacttg atgcaggaca gctttttcac gacctctaat tcacggtgtt caagcaagaa
    56821 atcattgttc actgaaaagc gttggatgtt ctttttcaaa ggtgtctaag atctctcgta
    56881 cttcatcagt agatttcgta ttcatcaact gaactcgcaa gtcatttgct cctggaaatc
    56941 ctttgatata gattttaaag aaacgatgta gtcctgtgat ggaacgcggg atgatttctg
    57001 aataatgatc ttgcagatct agttgcagac gaagtagtcc gatcagttct ttttctgtat
    57061 gttccctcgg ttctttttca aatgcataag gattcttgaa aatcccccgg ccaatcatga
    57121 tgccatctac gccatatttt tcggccaatt cttctcctgt ctggcgatct gggatatctc
    57181 cgttgatcgt gatcagtgtt tgcggagcga tttggtcacg taaagcaatg attttaggga
    57241 tgacttccca atgtgcatct actttactca tctctttacg cgtacgcaaa tggacagaaa
    57301 gattggctat atcttgatga agtaggtgac tgatccattc ttccatctca gacatttccg
    57361 tatagccgat acgcgtcttg acgctgacag gcaaaccgcc agctttagcc gcttcaatca
    57421 attgtgccgc tacttctggg cgaaggatca agccactgcc ttttccacgt tctgctacat
    57481 tagggactgg gcagcccatg ttgatatcga ttccttggaa gcccatttct gccatagcaa
    57541 tgctcatttc tctaaaaaat tctggcttgt ctccccaaat atgggcaacc atcggctgtt
    57601 catcttctgt aaaggtcaat cttcctcgta cactgtcttt tccatctgga tgacagaaac
    57661 tgtctgagtt tgtaaactca gtaaagaaga catccggtgc tgctgcatgc tttacgacat
    57721 ggcgaaatac aacatctgtt acatcttcca ttggtgctaa aacaaaaaaa ggctttggca
    57781 gctctttcca aaaattagta gtcatttatt tgttactctc cattctatca agcagtgaag
    57841 tcgcaatctt tctcaaaaac attgtcatta tactaggaaa caaaaaaaga agcaaagtga
    57901 acagcttaaa agatgtttct gattgagcaa aaaaagactg tgacaaaaat cttatcacaa
    57961 ttccttttcc ggaataaatg gtgttcaaaa gcttgcatct gcggtaataa gtccaaccaa
    58021 acgaaaaata ggaggagcta tttttgtttg gctgttctta ttacttgatg cagaacagct
    58081 ttttcacaac ctctccacta aacggtgttc aatcagctga ccctcggtat tagctcaaca
    58141 aacgacaaaa tatgaggaac tattttcccg tttgtttctc taatactcag gtcaaaacgc
    58201 tgatttcaca acctcttttc taacttctca atcctcttcc tttattgatc aaataccaag
    58261 taacggcgta caaaacaacg atgaatagca caagtacgat catcgatgtt gctactggca
    58321 catcgattgt tccaaggaat ccgtaacgga aaccgaagat catataaacg atcggattga
    58381 ctttcgaaac agcttgccaa aatggcggca acatagagat ggcatagaaa acgccaccta
    58441 gataagttaa cggctgcaag acaaatgtag gcacgatcga tacatcgtca taagactgag
    58501 caaaaatccc gttgagcagt cctgctaacg aaaacaagat tgccgtcatc aataatgtaa
    58561 tgatgaccat gaaccatgaa tacacatgaa gtggtacaaa gaacaaggaa atgatcgtta
    58621 cgagagagcc gacgagaata ctgcgaccca ctccgccaat cacaaagccc caaatgatca
    58681 catgcgtagg aacaggcgct accaagattt cttcgatgtt tttctggaat ttttgagaga
    58741 agaaggacga agaaacgttg gcatacgaac tagtgatagc agacatcata atcaaaccag
    58801 gcacgatgaa ttccatatat gaaaaaccac ccatgtcacc gatccgacca ccgatcattt
    58861 ttccgaaaat aacaaagtac aaggatgtcg tgatcacagg cggaaccagt gtttggaccc
    58921 agatacgcaa atagcggttc gtttccttga tagctaaact ttttaatgca gtaaaataaa
    58981 gattaaacat ttttctcctc cgtatgatta ttttcttctg tgattttcaa aaacagttct
    59041 tctaaacgat tcgacttatt cctcatagaa agaactttga ttccttgtct tgtcagctga
    59101 tcgaagattc cgttcacccc ttgatttcgt tcaacttcga cagcgattgt ctgttcatct
    59161 tcaaatgtac aagcgtatcc ttcaatcaca ggtttggtct catatggcgc taaatcaaag
    59221 ataaatgttt caaattgcag tttggataga agctctttca tactagtatt ctcgatcagt
    59281 tcacctgatt ggatgatccc gatgttgcga caaagcattt cagcttcttc taaataatgc
    59341 gtcgtcaaaa tgatcgttgt accattttcg ttcaattctc ggagaaactc ccacatctcg
    59401 cgtctcaatt caatatcgac tcctgctgtt ggttcatcca agatcagcaa acgtggttca
    59461 tgcatcaatg ccctagcaat catcagtctt cgcttcattc ccccggaaag catacgtgca
    59521 cggacatttc tcttttccca aagattggac tgcttcaaat atttttcgct gcgtttcatc
    59581 gcttctttgc gagagacacc gtagtagcct gcttgattca cgacgatttg ctggaccgtt
    59641 tcaaagggat tgaaattgaa ctcttgcggg actaatccaa tctgttgctt tgctcggact
    59701 agatccgtat ccagatcata accgaagaca ttcacttttc cagatgtttt attgactaaa
    59761 gaagtaatga ttccgatcgt tgtagacttc ccagctccat ttggtcctaa tagcgcatag
    59821 aaatctcctt ctttcacgga aagatcgatt cctcgcaatg cttcgacccc acttccataa
    59881 acctttttca aattatttat ttcaagcgca tttgccattt atttcctatc tccttataga
    59941 catgattctt tattgtatca tggaatacag cagatgaaaa aacaacttgg ttccagcgaa
    60001 aaatcaggtt gctgggctca ggaaaatctc ctaatgaaag acatcaatcg gacgaagtca
    60061 gacaaccttt tcagaaaaaa caaatcgaag ataaaggatt actattcata tgtgcaacaa
    60121 aaggagcgtg agacaaaagt aaagaacact tttgtctcac gctctaaaca cgtataaacg
    60181 gcggaagcaa aagccaagat ttttgatcca ataaatactt tcgatcactt cgcctatccc
    60241 aatacatgcc actgccgctt ttcatcaaat aacgcggctt ccgtttttgc ggtttctact
    60301 aaagtctctg ggtaatccaa aactttcagc aaggcaatcg catttcgggt ccttgaaggt
    60361 ccttctttta atttgaaatc aaaactgatt ccttttcctt ctgttacttg ttcttcaaag
    60421 tggacattct cacaataatt ctttaaaatc tctgtcaatt cgatatcatg tgtggcgaca
    60481 aatgctaaag atggatactc cgcaagccac cgaacgactg aagcggaaga agcaatccgt
    60541 tcgatcgtat ttgtcccttt taaaatctca tctacgaagc agtagcagcg ttctttggaa
    60601 gccacttggt ccaacaaacg tttgatcgat ttgatttctg aaacaaaata actgtcgcct
    60661 tcaaataaat catcttcgat tgccatggaa gtcaacacat gtccaggttg cagggtaaac
    60721 tgttctgcaa tcgctgtttg gatcgtctgt gctagaatac agctgatggc tatacttttg
    60781 acatacgtag attttcctga tgcattggaa cccgtgacta atgtattctt ctgccagttg
    60841 accggattca ctaccgcttc ttttaacaat ggatgataga tatctttcgc tgtcacaccg
    60901 ccctcagtaa actctggtcg gcaagtgatg ggcatataag tacggtaatt caatattgca
    60961 gcggctactt ctaattctcc cagcaattcc cacagcttga tggcttcagt tgaatgcttt
    61021 tctaatttag tgagaacgaa atgataggag ataaatggca gcatgaacat gatgttgaca
    61081 tattcaaaaa tcaactctgc ttcactccca ctcttcatac gaaatgaaaa acctaattta
    61141 gggatgtctc ttaatggttt agtttgctgt ttcagctctt cttgaagtgg tgtcggcagt
    61201 tttgctagct gattggcggc tgaaatcgtt gaaaccaaat aactcatgct attcagctct
    61261 gtttctagtg tttgtttctt cactagataa tagaccgtat tgaatactaa cccaccgatt
    61321 gccaaataga ttcctagtac ttgtcctaaa agaagcaaac ccaaacccac cagcggaagc
    61381 aagccaagaa aaataaataa acctacgcgt ccaatctttt tactttttcc atccgctaga
    61441 tattgttttg caaaattatg atcttttttt cctaaacgag cgaattgata ttggattttt
    61501 tctctcagtt gaggattttc ctgataaaaa gcaatcagtt tttctaactg ctgatcttca
    61561 ccaaaatcaa agctgcgtaa acgttgatac aacgcttctg aacctacact agaataggta
    61621 ctgttgattc cttcgaatac ttcgaacata tccaggtcat accaagtaag atcatcgatt
    61681 tcgctatccc atgaacggaa cttcttctcc gtgagccatg cttccttcaa gctttcttct
    61741 ttatcaaatc tcggttgata aggaattttt tcccatttgc tacgaactgt ttcctttaat
    61801 ttcaaacgtg aaaaaatgtc taaagcaact aataaaataa cagctgcaat aataattaaa
    61861 acgataaatt ctctttgacc catatccctc tccgttcctt tatttgttag ctttagtata
    61921 gcactgttgc gctatttgaa aagagggtaa aaaaacacct ttcaaaataa aaggtgtttt
    61981 tttcttgtat attacatatt tatctctcat ttatcaaaat tgacaaataa tcatagggag
    62041 gaattgttgt taattcttgt ttattctcca gtgcattgac aactgtccca agggaaagga
    62101 tagcctcagg tactattttt tcttgttctg ttggattagc taagcgaaga acaaatcctt
    62161 gttcatgaaa ataagaagga taaatcgatg atacaattaa gtcttcaggt aattcaatca
    62221 atgaaaattt acgaggaaca tcaagttcat tttgtaatgg ccaaatcttg ttatctaacc
    62281 tgtgaataaa tacattcagt ttctgctttt gataagaaat agattgttct aaccgttttt
    62341 ctgcctgttt tgccactttg aactcattaa atcttccttc ttctacatta aatccaaaag
    62401 tgactttata ctcacctatc tcttgagcta gcggtgtttc catcatgaca tgcccttcat
    62461 ttgtcgtatc tcctgaagct cgcccaggtc gccaagcaag attaggtttt ccaagttccc
    62521 ctgttgttga aaataaggtt atgattaatt gattttctat acgttcatac tcttttaaac
    62581 catctgcata aaacgtaaaa taatgatcat tttctgccac agagacactc ttatcgaata
    62641 tttctaaatt aacaggtttc tctaccatag tttctgacca attcggagga atgacatcag
    62701 gggtattttc aataaatccg ttttgaattt gtgcaattga tttttcagat ttatctaaaa
    62761 cattaattgc taagcgcata cgatgcgagt aaacagtatt atgaattttg agtgtgcctt
    62821 caacgatttg actttctccc agactgaacg atatttcata agatatttct tccatttcgg
    62881 gtttttcaga taaacgatct tttaaatctt taggaagttg agcttttccg taaacaacta
    62941 atgtttcttg taaagaatct ttatatactt tagctgtttc taattttaga gatagttctg
    63001 tatctccttc taatggtgaa taatcatacg tatcgccatc atttgctgaa tcgatcaaat
    63061 gaacgaaatc aagatattgt ctatctccaa ctatcaaatt caattgccca tctttaaaag
    63121 aaagtttata tttattgtta cttatctcag ttttgtctat ctcgattgct gtttctaatt
    63181 tttcattatt tttctcaaat tcaacaacag tgtatcctag tgctggcaca tttacattta
    63241 tttttatgtc aagttcataa taagctggtt catcaaaata ttcgaaacct gtagcaatca
    63301 tccgataagc atttttccgt tctggaaaat aaacttcttt ttcaatgata ttatagggac
    63361 tacctttgaa gtgtattttc tttgaatcag ctactacatg cacgattttc gatccactat
    63421 aattctcagt cgttgtgttg aaaacaagga cttcattcga ttttagcttt aatgcatcag
    63481 ccatccgttt tacaataagg ttttcgattc cgtcagctat ctcattagct tctttgattc
    63541 gatggaaaat atcttccgca acattatctg aaacacagcc accaatacta tcatgagcct
    63601 ggttttgcaa aatctttttc cataaccgac gaagtaggcc tttactgata tggataccat
    63661 ttctttctgc aatcagcatg agcggctcaa ctcgttttag aattttttgt tccaatttaa
    63721 aattttctat tttcattttt gttcttactg agccaataga acgatgaaca cgagagtaaa
    63781 ccggctgacg aagttcacct ttatattcag gtaattcttt tttgtttctg ataacttcta
    63841 cataagaagg ataatctgaa ataatattgt ggaatttact gtatttattc aacttctcta
    63901 cgacttgatc aaaatttaaa atcattgact tttgatctac acccgaaggt actaaaatat
    63961 ctttgttttt tccttgcata tgaagcattt caactgcttc atccaacttt tcttttgcaa
    64021 attcaggaat atctttaatt gcgtctaagg tcatcattcc agtcatataa ccaaatggaa
    64081 aattcatggc ataaacaaat ttatctccca atcctttcca gataaaataa ggagatggaa
    64141 ccatcgtttc aaagtttaat cctcgccaac acatgaaatt atcgatttct acttgatgca
    64201 aaagtgtagg caaattcgca ttgaacccaa acgtgtcagg caaataccct accatcattg
    64261 gatctccata tttaaggatc gtatcactga ttccaatcat taagtttctt aaaattgatt
    64321 cagcatccac taataaggca tctgtttgtg cataccacgg tcccacgaat agttgtcctg
    64381 ttttcatcag tcgttggata accttaagct tttgcggatt gatctccaaa tactcatcga
    64441 cgattgaagt ttgtccatct aaacaaaagt ttactttagg gttcctttcc aattcatcta
    64501 gaacttcagt aaagacctga tcagacaata cgatggcatc ttcatttgta aaataccatt
    64561 ctctgtccca gtgcgtgtgt tgaattatat gtgcatttac catatcagat tctcctactt
    64621 tccttcttct actgaattta cttgttgttc ttttttatac ttgatggatt ttagtgcatt
    64681 gaccattaac gctgtaacca ctcccccaat aacaattgat actaaatata aaggtatatt
    64741 cgtaacaaaa ggaattgcca taaaggttga aagcattgtt tcttgagtaa caccaaaaaa
    64801 tccatttaga ccacatgcta ctgcagtgcc caccacgatg cttggtatta ctcgtaaagg
    64861 atctttcacg gcaaacggga tcgtcccttc agtaatcccc acaaggccca taatcatagc
    64921 tgtctttgca ttatctcgtt ccgattcatc ataaagtttt ttgtttaaaa ttgttgctaa
    64981 aaacattcca aatggcggaa tagcaattcc taataacatg gctgtactag gtccaaagct
    65041 tccgcttgaa aaagaagcaa ccccaaaagc atatgcgatt ttattgatag gtcctcccat
    65101 atcaaacgcc atcattcctc ctaaaatagc cgacatcaaa gcagcatttg ctcctgacat
    65161 gccttctagc caattagtca gtcctgttgt cattgcttga ataggcgtag ctagtacata
    65221 ccataatatg attccaacaa ctaatgtagt aacaaaagga atcagcataa ttgattttag
    65281 actaataact tgcatcggta cttttacttt tttcaatgca ttagctaaat agccacaagc
    65341 taatccagca accattcctc caataaatcc cataccaaga ttttgacaca tcatcccagt
    65401 gataaaagct ggtgctagac cgggacggtc agcaattgaa tatgcgatat aggctgaaat
    65461 catcggtacg ataaactgta atcccgtctg accaatcatt cgcaggactc cccagaatgt
    65521 atccgtctgg tcccaaacac ctgtgccacc aaaaatgact gcaatagaag ttagtaaccc
    65581 tgctgctacg acaagcggga tcatatagga aaccccactt tggaaatgat ttagaatttc
    65641 tcttccttgt ttcttgaatt ccattagtaa ctcccccacg atatattttt tatttgttaa
    65701 ttaatgcaat tgcttcatca atcgtctctt caccatcttt tactacctct gaggtcccta
    65761 ctgttaaaat cggtttaccg tcaaagcgac ctcgttctga aacatttgta tctactgcaa
    65821 atatgacaac atctgcctcg gaaacatccg aaattgaaag tttgttttct atccccatag
    65881 ccccctgtgt ttctacttta atatctaacc ctttcttctt cgcaaacttt tctaaattag
    65941 cttttgccat ataagtatgg gcaatccctg cgatacatgc tgttactgct actatttttg
    66001 tcatatgaaa ttcctccttt taaaatgata gagcttttac caattctttt ttatttctcg
    66061 aatcttttat tcttttaaca aactcgtcat ccatcaatct tccagataat tctgctaaaa
    66121 tacgtaaatg attagtacct ttatcagtgt cttttattgc taacaaaaag acgagatcaa
    66181 ctggttgatc atctaatgcg ccccattcaa cagaccgctt taacttggct acagccactg
    66241 ttgattcaat tactgctagg cttttgccat gaggaatggc gatatttttc ccaattcctg
    66301 tggtcatatg gttttctctg tcaaatacgt ctttttgaaa ttgtttgtgg tcagacacaa
    66361 acccattagt ctctaacaat ttcgataatt tttcgactac acttttttta tcagatgcat
    66421 ttaaatctat cactatatta tttatattga ttttttcact gatcatttat aagccttctt
    66481 tcaatatata aactaatttt tcaaacgacg aactcgtttt taaatttgct aataactcat
    66541 gattcgaagc gatttcattg aatgtttcat aaattttatt caattcaggt tttacttttt
    66601 cattaagtgc aaaaaagaaa acagaatgaa caagtgtccc atcccaatca attccttctg
    66661 gattaacgta gacacagatt actggctttt ttataaaatc tacttcgata tgcggcatag
    66721 caaaatcatt catcgctgtt gaagatagtt tttctctttt gattgctccc tctagtatcc
    66781 cttctttcgc gtaattttct tttaccaatt ttttggtaat tatatttaaa acgttcactt
    66841 gattatcctt tatttggtgg gtaaaaaagc aaaatcgttt atctaataaa tttagaaaag
    66901 catcttctgt ttgttcattt aactgtagat actgttttac ttttctgaca tctgccccta
    66961 acatcaaagg actaacaaca ataacaggaa aatttgtatt ttcaatcgga atcgtgctta
    67021 taacaagttc atcagtgact tttctatctt gtagttcatt gatgcttagt acatcttcta
    67081 tctcaaccat atctgcaaaa ccgttattta ttctttgctc taataatttt gatgttccat
    67141 atccgctact acaaactaat atcactttcg ttttgtcagg tttgtaacga tccaaaagtg
    67201 attgaatatg gagtgttacg taagcgattt catcttcatt aaaatgtata cagaatctct
    67261 cttcgatttc ctctaataaa tctacactta tcataaatga ccttttaaaa gaatatttga
    67321 ttttgtccgt ataagggtta tagatattta ctcccaattt gagtctttta actgctgcat
    67381 tgagatgtaa tactagatta ttgatcaatt cttcatcagg ataaaattca tttaatttaa
    67441 ttcttactaa ctcttgtagc tcaacaccca tatctaaata ggaaagcttc gtttgatcta
    67501 agtcgtgatt catagcattt ttttcaatcg aagtaatgtg aatgtcaaca taagcttgtt
    67561 catctattgg cacaataatt gaaaatgctt tttcaatcat tttgattaaa ataagtgtat
    67621 tctttgagat ttcttcttca gaattattac tacgtgataa gaaattgctt gtttcaatcc
    67681 tttctatcgc aatagcaagg tggatagcta aagtttgaaa ttcataatct gtaaatttca
    67741 attgtgtctt attcatgtat cgattaagca attcggctac ttttttcaat atgtccttat
    67801 ttaagaaatt attcgcttca ccaatgaaat tgatattcaa gtctaagaga tctgttttac
    67861 tgttagatgc tgtaaagcca ccccaataat agttcacaac ttctgacatc aaccttcttt
    67921 tacttttttc atcagctttg atgcgcacgc catttcgatc tgaaactaag gacatcccat
    67981 gcttgtttag tctttttcta acatctttca ttgtgctttc taccgtcgac ctgctcacgt
    68041 ataattcgtc agcaagattt tgaatcttta gagaatcatt agtattcaat aatttagcaa
    68101 acacataagt tagacggtcc tccggagagt ctgcgatatt gattttatga ctggataata
    68161 aatttcgaag tgtttctttt tttccaataa gcgaaacgcc ttttttaggt tgaaccaaca
    68221 tttgtacatc tggcattttt tggattattt ctttcaaaat taaaatatct ttgctaattg
    68281 ttttcgtaga tacatataat tgttctgata attctttgta agttgtgtgc tcattttcaa
    68341 tcaaaaagta tagtattttc atttgccgtt cgctaaattc gatgcccatt atcttcacct
    68401 cctgaataca gcttaagtgt acaattatta tctctatttt catacagaag aaagttcttg
    68461 attaatgtag aagtttctaa tattcttgat tatttcaaat caataaaaaa gcctgcaata
    68521 tttatatcac aggctttttt ctctattaaa tatatataag ataatacatt tttctactag
    68581 ttctcactaa tcttgtataa ttaaaccctc cgcaacttga cggagatgga gaagatgccc
    68641 ccgtctaatc tagcggtcat ctctcctccc attccttcca ccaatttttt tgacaggtaa
    68701 agcccgattc cgctagattc cgtatttgtg cggctggtat tttcggtata aaaacgctgg
    68761 aagacttttt caacagaggt ttgcgttcct ggtttgatag gattggccac tgttaatcga
    68821 atgaatggat cctcttttga aaatgtcacc tttgcttgtc catctgcata acgcaataaa
    68881 ttctgtatga tattttgcag cactcgcagc aacatgagcg gatctgctaa tacagttgta
    68941 tttatcgagt gttcagcgaa cgctacctcg atatttttct cagaaaattg ttcataaaaa
    69001 gagaaaaata cttcttccac ctctttagaa aaagaaagcg tatggagatc catttctttt
    69061 tgcttcgtct ctattcgagc aagatcataa aactgatccg tcatactgag taatcttgca
    69121 acagattgtt tgatgatcgt taaatacttc tcccgtttct ccgtatcctc tacctgttcc
    69181 agtaattgta cataaccgtt aatagcagtc aaaggagttc tgagatcatg agaaaggccg
    69241 acgatcgata gtctgatgtt ttcttccatt tcttgaaatg cttgtccttt ttgttcattt
    69301 tcatccaacc atgtattcag tgcatctact aggtccatta gattcttttc tcgaaattcg
    69361 actgaaagac gaccaccata ttcttgtcgt tcagaaagag tggcgatttt cttagttaat
    69421 ctcttgattt ccctttgcag gaagaaaata aaacttccca taaagataac aacgagaagc
    69481 aacagccatt taatcaaatc taaaccccac tccccacacc gtatgaatat acacagcatg
    69541 tgcctttgct agcttccctc gaagattgga aatatgcata ttcactgttt tctcactatc
    69601 aaaatatggt tcttgccaaa ctgtttcata aagatttgct cgcgaaaata cttttttcgg
    69661 atgttctaaa aaaagcttca gcagatcgaa ttctcttcct gtcaaatgga cagccacacc
    69721 attcacattt acatccctcg actctttgtc cagtaagatt tccttgtaat agatgcgttg
    69781 cgacagatct tttacttgga cagaagtctg atgacgaagt tggatgccga ttcgtaacaa
    69841 aagttcttcg atatcaaatg gtttcgtcac atagtcatcc gctcctgctg ccaacaagga
    69901 aactttatct ctttgatcgc tttttgctga aagaacgatg atcggaacat tcgatgtctg
    69961 tcggatttct gctaacaatg cttcacctga aagccctgga agcatgagat ctagaaggat
    70021 caaatcgaca ggatatgtgc ttaacaataa ttttccttcc gtacctgaat aagcatcaag
    70081 aagtttgtat ctctgttcaa gaacctcctc cagtaaggaa tgaacagctt catcgtcttc
    70141 gataatcaaa atagtttcca gcaggaccac ctacttaatg tcagattttt ggaaaataaa
    70201 cattcctaga gctgatgata ccacgattgt tactagactg accagaatca ttttcggtgc
    70261 ttgcgaataa gttgcttgtt gcgcaaagtc cgtatacatg agccacgttt ccgtaaacca
    70321 ctccgttaga aattccaagc ctttgatttt tgcgaaaaca ccgggaatga aggccgttcc
    70381 catgatataa agaaaagaaa gaagcaaact gacactggta cttctagtga tgaatgtcaa
    70441 agccactgga acagataatg tccctaatgt cagcagaagc aatacaggaa atacttttag
    70501 aacataattt ccatagtttt tgattccctc aacttcacca aatccgaaag caagtgtgta
    70561 agcaagcatg gctagtcccg tgatcaaaac cacgccaagt gttgtgatca aaagcgtaac
    70621 gagaagtttc gacacataat aagtcattcg attccgtcct aaagataacg tattgcgaat
    70681 cgtaccgttt tgaaattctt ccccaatata aaagcacgca aatgctgcga aagccagaag
    70741 aatccatgca gccatttttt ctaccattaa aatccctgtt gcaccgttga tgactgtatc
    70801 cgttgcttgg tttaattcat tgccgtattt tgtatgcata taccattcgt tccaagaata
    70861 aaaaagcgga agtacgacca tcagtcctag tgagacccac attttacggc tcttccatac
    70921 ttttattttt tcagcagtta atagattaaa catgtggatt gcctccagtt aattgtaaga
    70981 aataatcttc cagtttttgc ccaactaggt gaataccatc cacaggaact tttgcttcta
    71041 ccaatgcttg attgatggct gctacttggt caattccttc aaaaagctgg ataacacggc
    71101 tagattgtac aaaataatcc ctataaccaa gagagtcgag aaccgttact gcttgttccg
    71161 tcgccgaagt gtcaattttt ataaattgac gagattcttg tgccaattct tctttcgata
    71221 attctttgat caagtgtcct tcatgcaaaa taccatagtg agtggcgatc tgtgacaatt
    71281 catctagcaa gtggctagaa agcaagacgg tgatgccccg ttcttttacc aaacgttgga
    71341 tgatttctcg catttctgcc atccctgctg gatccatccc attcgtcggt tcatccaaaa
    71401 tcaaaaattc tggattcgtg atcagtgcat ttgcaatagc taaacgctgg cgcataccta
    71461 aagagaaatt ctttgctttc tttttccctg tattttccaa tcgcatcatt ttcagcaggt
    71521 cttcgatttc tctatccgac acaccgccgt tcgctgcttg gatacgtagg ttatctcttg
    71581 cagttaattc tggataaaga gcaggggttt caatcgattg acccatacgt cgtcttgctg
    71641 cctgcaaatc cgtcgttcca aataattcga tcgaaccttc atcaatcgag atcaaaccca
    71701 taatagttct cataaaggta gacttacccg ctccattttc cccaatcaat ccatagatca
    71761 ttccacgttt gatttcgatt gatactttat ctaatgcttt ggcagcaccg tattttttgg
    71821 ttataccagt tgctttcaag accgtttcat ttccaaaatc catacttatc cttctttctt
    71881 attgatgagt gaagtatagt tgacgggtat aaagaaagca gccagaaatg tataaagaaa
    71941 gtgtaaattt tccaattgaa tgtcaaaaaa aacaatcgga caatacgtgt ctttcctatt
    72001 atcaggcagt ttgttatacg cttgtatcag tttcctgatt ctttcttttc gaccaaagtc
    72061 gtttctctgt attcctcttc actgaaaatt tttgcttctt tcagatcaag cttgagtaat
    72121 tttaccagac cggttataag gataactata gcaagactcc ctcccagaaa aattagactg
    72181 tctcgcagac gtggttgcgt tgtcttccaa tcaaagagcg caccaaacag tgctaatcca
    72241 agtggactgg ccaccatggt aatcgttgtt atcgttgaca taagtcgtcc ttgtatatgc
    72301 gttggaactt ctcgctgaag ccagaccata taaaaagcaa aagaaaacat taatccttaa
    72361 aaacggactt atgaaaaata atacatgccg tttgggattt acaattagct accaaataag
    72421 aggttatgac aaaagacttt tcacaatccc ttcttccgaa taaacggtgt tctcctgcca
    72481 actcctcggt cacaagtcac aatatttatc aaacatgaga agcaattttt gtaaattcct
    72541 tcttatttct tgaagctgac cacttctgtc acaacctcag aagcggataa ataatattgt
    72601 ttgatgatac tttatctttt tcccgtttgt ttatagatcg aatgatatta tagaccaaga
    72661 atcctaccta ttaacatagc taaaaacaca aaaaaggcac tttccaaatt gtggaaagtg
    72721 ccagaaaagt taggattaag gtaactgcgc tcagcattgc ttcgcccagt acgattctcg
    72781 taactcgtga actcgaacga taatcgcaat taacgttttg agaactgtga agctttacgg
    72841 gctttcttaa gacctggttt tttcttcgta tcattgaata agtttgaaaa accttgattt
    72901 ataaaggttt tcccgtaaat atattttatt atatttgatt aaatttgaaa tgtt
//
LOCUS       DAZKKW010000002        72814 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.002, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000002 DAZKKW010000000
VERSION     DAZKKW010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 72814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 72814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..72814
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.002"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(19..729)
                     /locus_tag="ACG8PO_000069"
     CDS             complement(19..729)
                     /locus_tag="ACG8PO_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HFQ0205430.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            complement(726..2198)
                     /locus_tag="ACG8PO_000070"
     CDS             complement(726..2198)
                     /locus_tag="ACG8PO_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HFQ0205431.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            2336..2809
                     /locus_tag="ACG8PO_000071"
     CDS             2336..2809
                     /locus_tag="ACG8PO_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFQ0205432.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            complement(2962..3513)
                     /locus_tag="ACG8PO_000072"
     CDS             complement(2962..3513)
                     /locus_tag="ACG8PO_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFQ0205433.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            3618..4148
                     /locus_tag="ACG8PO_000073"
     CDS             3618..4148
                     /locus_tag="ACG8PO_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HFQ0205434.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            complement(4631..5074)
                     /locus_tag="ACG8PO_000074"
     CDS             complement(4631..5074)
                     /locus_tag="ACG8PO_000074"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0205435.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(5090..6250)
                     /locus_tag="ACG8PO_000075"
     CDS             complement(5090..6250)
                     /locus_tag="ACG8PO_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFQ0205436.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            6757..8457
                     /locus_tag="ACG8PO_000076"
     CDS             6757..8457
                     /locus_tag="ACG8PO_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0205437.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            complement(8559..9392)
                     /locus_tag="ACG8PO_000077"
     CDS             complement(8559..9392)
                     /locus_tag="ACG8PO_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFQ0205438.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(9696..11861)
                     /locus_tag="ACG8PO_000078"
     CDS             complement(9696..11861)
                     /locus_tag="ACG8PO_000078"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIBC"
                     /protein_id="HFQ0205439.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            12121..14415
                     /locus_tag="ACG8PO_000079"
     CDS             12121..14415
                     /locus_tag="ACG8PO_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HFQ0205440.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            14408..15073
                     /gene="pgmB"
                     /locus_tag="ACG8PO_000080"
     CDS             14408..15073
                     /gene="pgmB"
                     /locus_tag="ACG8PO_000080"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFQ0205441.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQTGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            15066..16073
                     /locus_tag="ACG8PO_000081"
     CDS             15066..16073
                     /locus_tag="ACG8PO_000081"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFQ0205442.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            16095..17114
                     /locus_tag="ACG8PO_000082"
     CDS             16095..17114
                     /locus_tag="ACG8PO_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0205443.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(17395..18762)
                     /gene="nhaC"
                     /locus_tag="ACG8PO_000083"
     CDS             complement(17395..18762)
                     /gene="nhaC"
                     /locus_tag="ACG8PO_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFQ0205444.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(18871..20292)
                     /gene="tyrP"
                     /locus_tag="ACG8PO_000084"
     CDS             complement(18871..20292)
                     /gene="tyrP"
                     /locus_tag="ACG8PO_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070908 - tyrosine:tyramine antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-tyramine antiporter"
                     /protein_id="HFQ0205445.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(20535..22412)
                     /gene="tdc"
                     /locus_tag="ACG8PO_000085"
     CDS             complement(20535..22412)
                     /gene="tdc"
                     /locus_tag="ACG8PO_000085"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004837 - tyrosine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HFQ0205446.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(22703..23959)
                     /gene="tyrS"
                     /locus_tag="ACG8PO_000086"
     CDS             complement(22703..23959)
                     /gene="tyrS"
                     /locus_tag="ACG8PO_000086"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFQ0205447.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     misc_binding    complement(24071..24276)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            24539..25840
                     /locus_tag="ACG8PO_000087"
     CDS             24539..25840
                     /locus_tag="ACG8PO_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFQ0205448.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(25909..26508)
                     /locus_tag="ACG8PO_000088"
     CDS             complement(25909..26508)
                     /locus_tag="ACG8PO_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0205449.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
     gene            26762..27643
                     /locus_tag="ACG8PO_000089"
     CDS             26762..27643
                     /locus_tag="ACG8PO_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFQ0205450.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            27656..29266
                     /locus_tag="ACG8PO_000090"
     CDS             27656..29266
                     /locus_tag="ACG8PO_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0205451.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(29343..30002)
                     /locus_tag="ACG8PO_000091"
     CDS             complement(29343..30002)
                     /locus_tag="ACG8PO_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFQ0205452.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(30285..30950)
                     /locus_tag="ACG8PO_000092"
     CDS             complement(30285..30950)
                     /locus_tag="ACG8PO_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HFQ0205453.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(31097..31702)
                     /gene="dhaL"
                     /locus_tag="ACG8PO_000093"
     CDS             complement(31097..31702)
                     /gene="dhaL"
                     /locus_tag="ACG8PO_000093"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HFQ0205454.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(31730..32740)
                     /gene="dhaK"
                     /locus_tag="ACG8PO_000094"
     CDS             complement(31730..32740)
                     /gene="dhaK"
                     /locus_tag="ACG8PO_000094"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HFQ0205455.1"
                     /translation="MPERKDGNKLKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRV
                     VAKNQKAEQVGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEAD
                     SGKGVLLIVKNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAG
                     TVLVHKIVGDAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDE
                     IEYGVGIHGEPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQ
                     FVFMNDVLALLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTI
                     SW"
     gene            complement(32715..33110)
                     /gene="dhaM"
                     /locus_tag="ACG8PO_000095"
     CDS             complement(32715..33110)
                     /gene="dhaM"
                     /locus_tag="ACG8PO_000095"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HFQ0205456.1"
                     /translation="MKKSILLISHSQKVTDGIKEMIEQMQKSEEVQIFSLGGINGEIG
                     SDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEEEQQQRYHLVDAPLVEGA
                     FAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            33264..33584
                     /locus_tag="ACG8PO_000096"
     CDS             33264..33584
                     /locus_tag="ACG8PO_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HFQ0205457.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(33696..34553)
                     /locus_tag="ACG8PO_000097"
     CDS             complement(33696..34553)
                     /locus_tag="ACG8PO_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFQ0205458.1"
                     /translation="MKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTISSSG
                     AENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELEEALE
                     AAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQQAKE
                     FNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAASFQRI
                     KDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGVLVAA
                     AYGIIRKKK"
     gene            complement(34661..35965)
                     /locus_tag="ACG8PO_000098"
     CDS             complement(34661..35965)
                     /locus_tag="ACG8PO_000098"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFQ0205459.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVSFIGVWCYVFL
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEYTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            complement(35962..36471)
                     /locus_tag="ACG8PO_000099"
     CDS             complement(35962..36471)
                     /locus_tag="ACG8PO_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0205460.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(36646..38781)
                     /locus_tag="ACG8PO_000100"
     CDS             complement(36646..38781)
                     /locus_tag="ACG8PO_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HFQ0205461.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(38864..40519)
                     /locus_tag="ACG8PO_000101"
     CDS             complement(38864..40519)
                     /locus_tag="ACG8PO_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFQ0205462.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(40521..40835)
                     /locus_tag="ACG8PO_000102"
     CDS             complement(40521..40835)
                     /locus_tag="ACG8PO_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFQ0205463.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            41005..41265
                     /locus_tag="ACG8PO_000103"
     CDS             41005..41265
                     /locus_tag="ACG8PO_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HFQ0205464.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            41279..41593
                     /locus_tag="ACG8PO_000104"
     CDS             41279..41593
                     /locus_tag="ACG8PO_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HFQ0205465.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(42323..42877)
                     /locus_tag="ACG8PO_000105"
     CDS             complement(42323..42877)
                     /locus_tag="ACG8PO_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFQ0205466.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(43003..44932)
                     /locus_tag="ACG8PO_000106"
                     /pseudo
     CDS             complement(43003..44932)
                     /locus_tag="ACG8PO_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
     gene            complement(45238..45954)
                     /locus_tag="ACG8PO_000107"
     CDS             complement(45238..45954)
                     /locus_tag="ACG8PO_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HFQ0205467.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(45955..47778)
                     /gene="glpO"
                     /locus_tag="ACG8PO_000108"
     CDS             complement(45955..47778)
                     /gene="glpO"
                     /locus_tag="ACG8PO_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HFQ0205468.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(47792..49288)
                     /gene="glpK"
                     /locus_tag="ACG8PO_000109"
     CDS             complement(47792..49288)
                     /gene="glpK"
                     /locus_tag="ACG8PO_000109"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HFQ0205469.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(49424..50884)
                     /locus_tag="ACG8PO_000110"
     CDS             complement(49424..50884)
                     /locus_tag="ACG8PO_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0205470.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(50874..52169)
                     /locus_tag="ACG8PO_000111"
     CDS             complement(50874..52169)
                     /locus_tag="ACG8PO_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFQ0205471.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(52501..53178)
                     /locus_tag="ACG8PO_000112"
     CDS             complement(52501..53178)
                     /locus_tag="ACG8PO_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HFQ0205472.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(53208..54299)
                     /locus_tag="ACG8PO_000113"
     CDS             complement(53208..54299)
                     /locus_tag="ACG8PO_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HFQ0205473.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEKAYKELYLPSPLYNFLKARYQ"
     gene            complement(54400..54834)
                     /locus_tag="ACG8PO_000114"
     CDS             complement(54400..54834)
                     /locus_tag="ACG8PO_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFQ0205474.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(55418..56875)
                     /locus_tag="ACG8PO_000115"
     CDS             complement(55418..56875)
                     /locus_tag="ACG8PO_000115"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFQ0205475.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(56872..58767)
                     /locus_tag="ACG8PO_000116"
     CDS             complement(56872..58767)
                     /locus_tag="ACG8PO_000116"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFQ0205476.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPIILLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSSVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(58825..60600)
                     /locus_tag="ACG8PO_000117"
     CDS             complement(58825..60600)
                     /locus_tag="ACG8PO_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFQ0205477.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARFPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(60650..62146)
                     /locus_tag="ACG8PO_000118"
     CDS             complement(60650..62146)
                     /locus_tag="ACG8PO_000118"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFQ0205478.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(62340..63962)
                     /locus_tag="ACG8PO_000119"
     CDS             complement(62340..63962)
                     /locus_tag="ACG8PO_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFQ0205479.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESIKAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTIPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(64290..65603)
                     /locus_tag="ACG8PO_000120"
     CDS             complement(64290..65603)
                     /locus_tag="ACG8PO_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFQ0205480.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(65848..66072)
                     /locus_tag="ACG8PO_000121"
     CDS             complement(65848..66072)
                     /locus_tag="ACG8PO_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0205481.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(66092..66514)
                     /locus_tag="ACG8PO_000122"
     CDS             complement(66092..66514)
                     /locus_tag="ACG8PO_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFQ0205482.1"
                     /translation="MESVYRQERKSNRKKGQMMIQKTRVMLYVNDVEMICRFFVEKIG
                     AEISETIELPEEFKSIVLSISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETI
                     YENMEEPGEITDNNGILTFNFSDPEGNYFVIGKAESSD"
     gene            complement(66656..67366)
                     /locus_tag="ACG8PO_000123"
     CDS             complement(66656..67366)
                     /locus_tag="ACG8PO_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HFQ0205483.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(67384..67881)
                     /locus_tag="ACG8PO_000124"
     CDS             complement(67384..67881)
                     /locus_tag="ACG8PO_000124"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0205484.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(67918..68370)
                     /locus_tag="ACG8PO_000125"
     CDS             complement(67918..68370)
                     /locus_tag="ACG8PO_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFQ0205485.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(68505..69110)
                     /locus_tag="ACG8PO_000126"
     CDS             complement(68505..69110)
                     /locus_tag="ACG8PO_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFQ0205486.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(69560..70180)
                     /locus_tag="ACG8PO_000127"
     CDS             complement(69560..70180)
                     /locus_tag="ACG8PO_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0205487.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLWQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(70182..71051)
                     /locus_tag="ACG8PO_000128"
     CDS             complement(70182..71051)
                     /locus_tag="ACG8PO_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFQ0205488.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(71074..71481)
                     /locus_tag="ACG8PO_000129"
     CDS             complement(71074..71481)
                     /locus_tag="ACG8PO_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFQ0205489.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(71500..72789)
                     /locus_tag="ACG8PO_000130"
     CDS             complement(71500..72789)
                     /locus_tag="ACG8PO_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFQ0205490.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFNNATATDVDTVAAALLYAWILSPVLKAF"
BASE COUNT        21063 a        15329 c        12669 g        23753 t
ORIGIN      
        1 tacatggtgt tcaaaatatt attcgccttt tgcttctttc actaattttg cgaatgcttg
       61 ttgttggaat tcagcagcta aggctttcgt acgttgttct tgctctgctt tgctcatttt
      121 tggattttca gtcattgctt ttaccatacc gtcttgaaca taagcttttg cttcactttc
      181 catcgtactt tgctgtttct tcactaattc agctaacttg gctgcttggt ccgtacttaa
      241 ttctacccat gtctctggga ttttgaatgt attcactcgg ctgtcatatt cgtgattgtt
      301 atcagcaata ttgaaccaga atttatacat gtcgtttttg tattcccaat aagtagtttt
      361 cgtcactttt tcagcatcgc cgtctgtctt ttctacttta tttgtttcgt tatccgtacc
      421 agttgctttt ggtttttctt gtttttcatt tgttcggtaa agatacactt tttcttttcc
      481 attccctaaa gctttataca aaagcatatc tgctccttgg gaatcagcac tggaaacaat
      541 tttttctgtt tttgtttccg ttactttttc cattccaaag tgattggaca aattagcgac
      601 gatcaaactt agtgaagcga cgaagattag tccgaaaatc aaagataaaa aggtttgcca
      661 tgttttttta gcgaacacgt tcgttaaagc aaaagcaagc aagctgacaa tcaaaactcc
      721 aataatcatg cattatcccc cgctttcatt agaacaggtt cttttttctt caataaaaag
      781 gcaataataa agccaagaat cccaaagatg atcgctacga taaacgcggc atggtaacct
      841 gacagtgtgg cattgattgc ctgatcttta taagctaatg gcgtattatc caatacagat
      901 ttgcttggga tatttgcttt tgtcacattc gtcaacacac tgatcaaaat agctgtacca
      961 atcgagcttg cgatttgacg gaatgtatta tttaccgctg tcccatgact gatcaagtta
     1021 tttggcaatg cattcatacc agaagtcgtt accggcatca tgaccatgga gatcccaaac
     1081 atacggatcg cataaaggat cacgatatat aaaactggag tcgtttttgt caagaaagca
     1141 aatggtgcag ttgccgctgt caaaatgaac atccctgtga tggctaaacg tttcgcgccg
     1201 tatttatcga aaatcgcacc cgtcacaggc atcatagccc ccatgatcaa tgcacccggc
     1261 aacaacatca agccagaatg gaatgctgat tctccacgaa tattttgaat atacaatggt
     1321 acgaccattt ctgcccctac catcgccata tttgttacac cagctaaaac agctgcaatc
     1381 gtgaatgtag atgatttgaa tacacgaagt tccaagaatg gatgttccaa atgtaattga
     1441 cgccatgaga acaaggcgat gaaaatcaca ccaacaacta agaaaccaat tactttcaag
     1501 cttccccagc cgtcatctcc tacactagaa aatccgtaaa gtaaactacc gaatccaatc
     1561 gttgataaaa tagcggataa aatatcaatg ctaggatttg acaatggaat aacacttttc
     1621 attaggaaga atgctaaaac caagacaaga acaacgattg gcagtaccat accaaacaag
     1681 tcacgccaat ggtagttatc aatgatataa ccagataatg tagggcctaa agcaggagca
     1741 agtccaataa cgatccctga catccccatt gctgatcctc ttttttctgg cgggaaaata
     1801 gatagcatga tattttgcaa taatggcatc gatactccca caccgcaagc ttggatcaaa
     1861 cgaccgatta gtaatgtact aaaattaggt gctgtaaagc aagtaatcgt tccgatcaaa
     1921 aagatcgtca tggcagaaag atataaattt cttgaactga atttattgat cagccatgct
     1981 gttattggga tcatgatccc gttgactagc aagaaaccag tcgttaacca ttgcacatca
     2041 gacgctgaaa tatcgaaagc tttcatcaat gtaggaaaag ctgtcgtcaa taaggtctga
     2101 ttcaaaacgg tacaaaatgt accaattaat aagacaacaa ctaaaagtgt ccgattatat
     2161 ggttttccgt aaatatctac gggttggttg ttactcatat ttctccttct ttctgaattt
     2221 tttctgaaac tactagaata ctttagtcct aaattcgcta tttgtcaacc tacttgacac
     2281 ataagttaat acggatataa ttaagggaag caagaggaca aaaggagtga caaaaatggt
     2341 tggggcttcc cttaaacgtt tgttggaaaa tgatcaattg gttgttggca tcagtgaatt
     2401 aagtgatatc gtaggtgtgt ctccacgtca attaaggtat tgggaacaaa agggattcat
     2461 tcgttcgatc gaagctgatg cgaattgtcc tagaaaatat cgtttaccta cagtcataaa
     2521 agtagaactg atcaaaaaat atttagatga cggatttact ttgtcaaaag ctgcagaaaa
     2581 ggcagaaatc cgccaaaaga aaatgcacca tgtccgtaaa gttttttcaa aagcaatcaa
     2641 agatatcgaa ttgatcgacg aacgctatac gattttacaa atcggtaact tcaaagaaga
     2701 gaatgctaag atgtacatca ttcatgacaa cgagacagac gaattaactt acaagctcct
     2761 tcctgtcgat gaaaaagctg actacgaaca gttgttaaaa gaactctaaa cgccactaat
     2821 ctcctaagta ccaagctttt taataagtag atgctacata aatacttgaa aaaaggcctg
     2881 tttgaacata agaatatccg aacggtgatc tgcgtttctt tcatatagaa aggaattctc
     2941 catcactgtt cggatatttt tttatttttt tcggttactt tcttctaata catctgttag
     3001 ccaatttgca tattctgttt cccgattgat cgcatgcgct aaaatcaggt agtgtccgta
     3061 attttcatgt ttttcttttt cagtaggaaa gatcgttttt tttcgctcta atagatgaag
     3121 tcgtttttct tcatgaaggt cacgctgctg ctgaataatt tctactaatg ccggatcatt
     3181 cacgtctttg acgaaataca atttcagaac aaattcgtct cgattaaccg gtagttcatt
     3241 ggtgggtgta tgaatccact ctgccaattg ttccttccct tcttctgtta attcataaac
     3301 tttcttttcc aatttttcac ctgtgatctc tacttgatgc tgaatgattc cttgctcttc
     3361 catttttgca agttcaggat aaatctgact atgttttgct tgccaaaatt ctcctatttc
     3421 atttttgaac gattgattca attcatatcc tgtcatcttc ttcttactta ataaccctaa
     3481 caaaatatat cgaagtgtat tttttcttgc catcagtttt ccctccttga caaatcttta
     3541 aatccattct atcataaatg agtaatcgaa aacatgtagt ttattacatg ttttaattta
     3601 taaggagatg cttaaacatg aaagaattta aagaattgga agattttatt ggaactcact
     3661 ttatctatac gtatgacaat ggttgggaat atgaatggta tgccaaaaat gaccatactg
     3721 ttgattaccg tatccatggc ggaatggtca aaggccgttg ggtaaaagac caagaagctg
     3781 atatcgttaa attgacggac ggcgtattca aaatcacttg gacagagcca acagggacag
     3841 acgtggcatt ggactttatg ccaaatgaaa agaaacttca cggaatgatc tttttcccta
     3901 aatgggttga agaacatcca gaaatcactg ttactttcca aaacgaacat atcgcagaaa
     3961 tggaagcagc tcgtgaaaaa tatgaaactt atccaaaatt acttgttcct gaatttgcta
     4021 cgatcactta tatgggtgat gcaggtatag acaatgaaga tgttattagc gaagaaccat
     4081 acgatggaat gccagatgat atccgtagcg gaaaatactt cgatgaaaat tataaacgag
     4141 taaaataagg ttgtgaaaga gaggttgtga aatcagcgtt ctgacctgag tattagagaa
     4201 agaaacggaa aaatagttcc tcatgttttg ccgttttttg agctaatacc gagggtcagc
     4261 tgattgaaca ccgtttatca agaggttgtg aaatcagcgt tatgctttga gtaataagaa
     4321 cgctagttag aaaatagctt ttcatatttt ctaggctagt gggcttatta ccgagaagca
     4381 gccgtttgaa caccgtttat caagaggttg tgaaatcagc gttttgacct gagtattaga
     4441 gaaacaaatg gaaaaatagt tcctcatatt ttgccgtttt ttgagctaat accgagggtc
     4501 agctgattga acaccgttta tcaaagattg taacaactgc atattcacac agttaaatta
     4561 gtgacttcaa aatgaaataa tgctcccagc aaaaaattag gctgggagca ttatttcgtt
     4621 attctgttca tcaaaaatca gcgttttgat tccatttcat cacaagttcc tcctcattta
     4681 ctgcttcatc tttttgctgt gtttgaattg taaaaccttg tgaaagatag aaatgaatag
     4741 ctgattgatt tttttgatag actgtcaaag tcagtgtttt attggtttct tttatttttt
     4801 tcattagttg cgtaccgatt ctttgttgcc ggtattccct tttcacaaag attcctgcga
     4861 taaactgatc aatcacccca gcaaaaccaa taatttcttg atctatttct gctaagtaaa
     4921 tcactgcatc tggtaaagct ttcctaacag attctacttg attctcccaa taagttgatg
     4981 gaatgaattc atgagaatcc aagttagcat gcagccaaat cgatagaaca gattccagtt
     5041 ctttggcaga caattttgtt gttgtgcgga tcattttttc tctccttact tattcattga
     5101 ttttaaaata aaaaccattt ttttgaagct cttttacaag ttttcgcccc tcaaaaaagt
     5161 atatcttctc ttgttttact tttctaatac caaaagaaac agcaacttta ggatcatcag
     5221 ctgaaacgat tcgataagtt ccaccaggca attgatccag cttgactttc ccatgatggt
     5281 cgctttgtac tgaaaacggc acaccatttc tagtaagtgt ttttttctta tgtttcaaaa
     5341 tgaatgaact accatttacc ggtttccctg acgaatcact aataataaat gaaaaatcaa
     5401 gcactttttt acttaatcta tactgataga ttagtcgtcc gataaacact aacagtatga
     5461 ctacagctat tatcgttcct gcaagaataa accattgccg catggttctt tcctgatttc
     5521 cttttccttc ttcttttttt accgattcgt tataaggaat ccgatgtcct gtcactagca
     5581 aacgatgcgt gttgatcata tacggcgtac atgtaagcaa ggtaactaag tcttctcctt
     5641 ctgaaatcgt tagaacagac gtgtcatctg gcagtaccac ttgtatattt ttaacttgat
     5701 aagccaattt ctcccccaaa acagtcaaaa taaacgtgtc tccgttttct aaacgatcta
     5761 attcggtaaa taaccgtcga ttaggtaaac cgctgtgagc agagatcact gaatgcgtcg
     5821 aatttccacc aagcgggaaa gaggttcctt gcaagacgcc tgctccattt tcaaggatcc
     5881 taccattgag ccgatcataa agtgaaatat ttaattggat cttaggtata ttgatacttc
     5941 cgatcagcca ttcctctaat tggctggatg ttgtactttc atttctgttc ttttcattga
     6001 aaggatcagt accaggattg aatccttggt cagttaatcg ttggtttttc tcctccattt
     6061 tctttcgctg cacttcaatt tcagctgcgg tccgtttttg tacttgttcg atacgttttt
     6121 gatcaataaa actatttaaa gaatccacat aaaatggata taaagcgatc agtaacccta
     6181 cgacgataca tattaataaa aacaaacgaa acaaaagtcg acgagactgt ttttgcttac
     6241 tctgcttcac atgtgtcctc ccttgcaagt ttttttgttc tttcttttat tatatgggtt
     6301 caggaaaaaa atagaaaatg aaagccgatg aggttgtgaa aaagctgtcc tgcatcaagt
     6361 aataagtaca gcccaaaaaa atagctactc atattttttg ttgggttggg cttattaccg
     6421 cagatgcaag cttttgaaca ccgtttaagg aagaggttgt gaaaactgcg ttatgctttg
     6481 agtaataaga gcgctagtca gaaaatagct tctcatattt tctaggctag tgagcttatt
     6541 accgagaagc agctgattga acaccgttta ccgagaggtc atgaaaaagc tactttgttt
     6601 cgagtattaa gaaaaattct gtttcaaaac aaggagatgc aacaaaactt atgtcgcatc
     6661 ctctctcctt agaactttca cttatgtttt tcttgtaaaa ttcataaata ttttcatgaa
     6721 ttttctaatt tcttgcctac ctctcgcata ggatatatga tgaatttatc taaagtttgc
     6781 aaaggagttc gacaattgaa aaaatatggc ccatttttag caattttttt cattgctttg
     6841 gcatttctca tgcctcattt attcacacat aaactaattt tgggtgctga tgccccattc
     6901 cattacaatc ggttctatga tgccgcgcaa caaattaaat atggggattt ccattatttt
     6961 ccttcccttt atggattcca gcaaagtggc aggatcgtga atgccgttta cggcccattt
     7021 ttcgcttatt tacaaggatt gatcgtgttg cttgcaggta gctggtttca ttatcagatt
     7081 ctttcaaatt tcattcttta tcttatttca ggattttcta tgctgatttt actgagatat
     7141 gtcaatgttc gaaattgggt aagtgtacca gtagccgttt tattcatgag tacgtattct
     7201 attcaatact ggtctatcaa ccaaggattc actagctggg ggacttgttt ttatccttta
     7261 tgtatgatcc ctattgctga tttcgtcctt cataaaaagt tccctgttat caaagtcgct
     7321 gtctcagtgg gacttatgac acagatccat ttactgtctt cgatcatgtt agctttgatg
     7381 tatatccctt tttatcttta ttactttttt aaccagactg aaaaaggaaa agcactgctt
     7441 caactgatta agtcggtctt gctttatttt gttctgacag caaatatttg ggcaggatta
     7501 gctattgttt acagtgggaa taagattctt cctccctttg ttaacagaga aatgagtcaa
     7561 aaggcgatag atctggatgg cagttattgg ctccattacc ctaaggcctt tcctatattg
     7621 atattatcag gttttttgct ttttattttc tttcaacaaa agaacacctt attccaaaaa
     7681 atcgtgttgt acacaagtct tgttttcttt tttctttcta ctagcttgat tccatggacg
     7741 acattgatcg aaaaaaatgt gccttttatt agtttgatcc agtttccatt tcgatttttc
     7801 gtgccattta cgctattgct tctactttac cttgcccttt cactgaacca gtggtctgaa
     7861 caaaagtggt tccgtctttt aagtatattt ggcattctta ttttttcagc acagacgatc
     7921 cagaacttat accagcatct ggaaaagtgg gaaaatgaaa catttgtcag tcgacatact
     7981 tatctattcg atacgccgga agaagcaagg aaaacattct tcagcaaaga tatgtctacc
     8041 agcttgttcg ttttccaaaa aaccacacct gactatctgc caatctataa ggagacaaaa
     8101 gacaataaat acgaccggta taacgaagaa atactagaaa acgaaacaac ttttataaaa
     8161 acacatccag gcggtctttt gacaatcaag tggaaaaaca ctggggacaa aactgttcat
     8221 atccctgtta tcgtctacga tcggactgtg ctacagcaaa atggaaaaac attgactgat
     8281 tatgaagtaa cagatatcgg gaccccaatc gtgaagcagc aaaaaggaat caacgagctc
     8341 acgcttcatt atcaaacgcc aatctatttc tatttcattc tttctcttac tttgattggc
     8401 tggtttacct tgctatgttt atttatttat catagataca agcttttacg tgcttaggca
     8461 aaagggataa atacatccga tcaaaaaact ctccaaacgg tataatcgtt tggagagttt
     8521 tattttaatc agttttttta tgttatatgt tatatttttt attcgttcac atctaattcg
     8581 atttccaccc cgtagtgatc actgatgatt ggactatcga tcccatcaaa aatccgttga
     8641 tattttttaa cctgcattcc ttcagggacg aaaataaaat ctatacgcaa tttttctgta
     8701 ttttgttccc aaccatctat tttcttttcc acagtagctg cttttccttt ttcattcgcg
     8761 acaacatacg aatcttgaag aagaaggcaa ctttctctta cagtataata accttgagag
     8821 tctactggat tattgaaatc acctaggaaa aaaagtggta cgcgagtttc caaaaagtag
     8881 ttttccaagt ttttccactc aaaataaaag ccactttttt tatctgtcca ccaagaaaag
     8941 tggcagctcg agacaaaaat gtcctgatca tctatctttg ttttcccaat caagatcttg
     9001 cgtgtctgat aatcttcttt cttttgaaat tgggagacgg taaggacctg actttccaat
     9061 gtttttttcg taagcagtgc atttccttct tcatatttat cataaccgat gtggctcatt
     9121 tcccaactcc aaaaatactc ttttccgtgt tcctttaaaa gctgaactaa acggtaggca
     9181 aaattatctt tacggatagg tacttgattc tttaccggac agaacttcat tagcttcgca
     9241 ggttctactt cttcagattc cagtaattgg ttgatttctt gtaaagcaat cacttcataa
     9301 tcttctaaca ggatcttatc tgctaattct tgcaattttt tctctggttc tggctcaagc
     9361 cagctatgcg tgttgagtgt catgaatttc attggttttc tccttgtctt catttcgaaa
     9421 caaaagggtt gtgacaaaat ttttgtcaca acctctccag caaatagtgt tctcctgcta
     9481 actcctcagt aataagctca atggaccagg agctattttt tgtttggctg tttttatcac
     9541 ttggtgcagg gcagcttttt cacgacctct ccactaaaca gtattcaaac tgctgcttct
     9601 cggtaataag cccaaccaaa cgaaaaatag gaggagctat ttttgtttgg ctgttcttat
     9661 gacttgatgc aggacagctt tttcacaacc tctccttaca ttttaatgga gccgatcact
     9721 ttaccagctg tcactgtacc taattgcttg atcactactt cttctacttt atcgtttgta
     9781 aatacgatca tgatcgttgg atctttttct tcgttttgga tcacatcaag atccatctca
     9841 gctaatttcg ttcctgttcc tacttcttgg ccttctttga cagcaatctc aaaagctggc
     9901 tgattcattt ggactgtatc gattcccata tggatcaaaa cttcgatacc atctttcgtt
     9961 tccagcccaa ttgcatgttt gctagggaaa atacttgtca cttttccagc gatcggcgca
    10021 acaacttctc ttgatgttgg aataacagca aagccgtcac ccatcatttt ttgtgagaat
    10081 accggatctt ttacttcaga taaaggaatc acttctccag aagctgcgga aactagttct
    10141 tgttctatac ctaagtattg cttactacct gcttgtccat ttcctgtaga aaccatttct
    10201 ggctctggaa tctctgctcc tgattgcagc agatcttcga tatctgattt caagacatct
    10261 gcttttggtc cataaactgc ttggactcca ttgtctttga cgatcaatcc catcgcccct
    10321 gctcgtttcc aggcttcttc agtccctact ttttctcgat catttacact tacgcgtaac
    10381 cgagtcatac aagcatcgac atctttgata ttttcttttc ctcccagcaa atgaatgatc
    10441 tggaccactt gctgatctgc tgttcctgtt ccgctgttag atacaacggt ttcttcactg
    10501 tcattgtcgt aatttccgtt acgtcccggt gtagcaaagt tgaatttttt gatcaagaaa
    10561 tttgctaaga aatacgtaac aacaccaaaa gcgatcacac acaacacgaa gttgataaga
    10621 tctccaccta gacccgcttt gatagctaac ggtgtccgag tcagcaattc gatattccca
    10681 aatgaatgga cccgcaatgg caagatatct gccatcgcaa atgcagcccc ttggatcact
    10741 gaataaatga catataatgg cacagcagca aacataaaca tgaactccaa tggttctgtt
    10801 acacctgtta gaaagacagc caacgctgct gaaaaataca ttgatttata tcttgctttc
    10861 ttatcaggat cgacattacg atacatcgca aaagccattc ccatcaagat acctgaagaa
    10921 ccaatcattt gtcctacttt gaaacgagcc ggcgtccagt tatgcaacac aaactcatat
    10981 tgcgacatat cccctgcacc tttaagattc actaaatcag tggcccaagc caaccataac
    11041 ggatcttgcc caaatacttg tgtccccgcc tgtgcgcctg ataaaatctc atacgatccg
    11101 ccaagctgtg tatagttgat aggaatcgtc agcatatgat gcaatccaaa tggcaatagt
    11161 aaacgttcta atgttccaaa taaaaacggt gctagaatcg gtgcgctttc ttgtgattct
    11221 gcaatccata aaccaaaatt attgattcca gcctgtacgt ttggccacac cacagcaaga
    11281 atcaatgcca cgattgttga ccataagata acaacaaacg gaacaaagcg ctttccattg
    11341 aaaaatgaca aagcatccgg caattttcta taattatagt acttattata agccattgcc
    11401 ccgacaaatc cagcgatgat ccctacgaat actcccatat tcaaagctgg tgcttccaat
    11461 acacttgtaa aaaatccttt aaccatgatc ttcgtaccga acagagtgtg ggtaaatgca
    11521 tcttcattcg caagcatgtc acttgtgaca ccaaagatag ccccagtgat ccgattgatt
    11581 aaaataaatg agatcccagc tgcaaatgct ccgcctgctc tttcttttgc ccaacttcct
    11641 cctattgcta aagcaaataa aatatgaaga ttaccaataa ttgcccaacc aatattttca
    11701 attactccgc ccgttgtgac aagcggcgtc caatcaggat tgatcaaggg aattgatttc
    11761 cctatactga tcatcaaccc agctgccggc ataactgcta ccacaaccat taaagccttt
    11821 ccaaattttt gccaaaactc aaaactaaat atctttttca ttccgtttag ctcctttacg
    11881 caatcggttt cgttaaacgt attatagcgt gcgttttctt ttttgtaaat gctttatttt
    11941 ttatttttct gtctatacca ttctataaaa cctataatac gtgtgttccc ttctataatt
    12001 ttctcctaaa aatttatttt tttaagcttt tttctatgaa atattttctt ttgtctattg
    12061 acttgtgcaa tcggttgcgt ttattatgaa cgtataaaac agctcattag gaggagaaaa
    12121 atgaaacaga tcaaacgact atttgctatt gatccatgga aaatcagcac gacacaatta
    12181 gataaaaaaa atcttcgact gcaagaatct ttaacaagta ttggaaacgg ttatatgggg
    12241 atgcgcggga actttgaaga aacttactca ggggaccacc atcaaggtac ttatttggcg
    12301 ggtgtctggt atcccgataa gacacgtgtc ggctggtgga aaaatggtta tccagaatac
    12361 tttggaaaag tgatcaacgc cttgaacttt atcgcgatag atatttcgat caacgatcag
    12421 ctgatcgact tggcttcttt ggaaccagaa gacttttact gggaattaga tatgaaaaat
    12481 ggtgtattat ctcgtacatt cacaatcact acttctacta ataaagtacg catttccttc
    12541 gaacgttttc tgagcattgt caaaaaagaa tcagcttata tctatttaac tatagaaatg
    12601 cttgaaggta gcggaacagt caaactcgta tcaaaattgg atggaaatgt tcaaaacgaa
    12661 gacagcaact atgatgaaca tttttggaat gagattgatc gtggaactat agaagaaatc
    12721 ggctatttga cgactaaaac tattccaaac aactttggga ttgaacaatt tactgttact
    12781 gcagcaatgc gccatttcgt taatggaaaa tcagtcatcc ctgactatca cgaagatact
    12841 ttagcattac atggttgctt gtctgcagaa ttatctcaag gtgagacatg gtcagtggaa
    12901 aaagaaatta tcgttcttac tagtagagat attccagaag cgcagcaagc agaaaaagct
    12961 gtctcagaat taacgaacca gacaaacaat tatatccaag caaaagaaga gcaaacagcg
    13021 gcttgggcag aacgatggaa gcttgccgat gtggtcatcg aaggagacga agaagcacaa
    13081 caaggtattc gattcaattt gttccaacta ttctctactt actacggcga agatgaacga
    13141 ttgaatatcg gccccaaagg attcacagga gaaaaatacg gcggagccac ttactgggat
    13201 acagaagcgt atgctgtccc tctttatctt gctttagcca aacctgaagt aactaagaat
    13261 ctgctgaaat atcggcataa tcaactgccg caagcaatcc acaacgctca gcaacaagga
    13321 ttaaaaggcg cgctttatcc tatggtcacc ttcactggtg tcgaatgcca taacgagtgg
    13381 gaaatcacat tcgaggagat tcaccgaaat ggcgcgattg cctatgcaat ctacaattat
    13441 acgaattaca ccggcgatac ggaatatttg aaaaatgaag gactggaagt tttagcagaa
    13501 atcgcacgct tctgggcaga ccgtgtccat ttttcaaaac gagcaaataa atacatgatc
    13561 cacggtgtga ccggcccaaa cgaatatgag aacaatatca ataacaattg gtatacaaat
    13621 acgatcgcag cttgggtctt gcgttacacg cgagaaaatt atttaactta tcaaaaagaa
    13681 gctgctatca ctatcagcga agaagaacta gccaaatgga aagatatcgc agagaacatg
    13741 tacttcccag ttgacgaaga attaggaatc tttgtccagc atgacacatt cttagataaa
    13801 gatctcatgc ctgtatccga tattcccaca agcgatcttc ctctgaatca gcattggtct
    13861 tgggacaaaa tccttagatc ttgcttcatc aagcaggcag atgtactaca ggggatttat
    13921 ttcttcaatg atacttttac aaaagaagaa aagcaaaaaa actttgattt ctatgaacca
    13981 atgactgtcc atgagtcttc cctttcgcca agtatccatt cgattttagc agcagaatta
    14041 ggaatggaaa gcaaagctgt agaaatgtac cagcgaacag ctcgcttgga tttggataat
    14101 tacaataacg acacagaaga cggcttgcac atcacttcca tgactggtag ctggttgtcg
    14161 attgtccaag gatttgctca aatgaaaact gcccatgatc agttgagatt cgcaccattt
    14221 ctgccaaaaa cttggaaagc ttattctttc catatcaatt atcgcggacg tttattgttc
    14281 atctcagtca cagatgaaca agttactttc gagttattag aaggttctcc tctagaaatt
    14341 tacgtctatg aacagtctta ttctttagcg gatcgtctag aaataacgct aagaaaggag
    14401 gaagcaaatg tttaaagggg tattgtttga tttagacggt gtcattaccg atactgctga
    14461 attccattat cgtgcttggc gaaagcttgg tcaggaaatc ggcattacga tcgaccacac
    14521 attcaacgaa cagctaaaag gcgtcagccg agaagactca ctctctttgc ttttagctta
    14581 tggcggaaaa gaacactctt tttcaaagga agaatttgca gaattagcga aaagaaaaaa
    14641 cgattattat ttagagatga tccagacgat cgaaccaaaa gatgtcttcc ccggggtcgt
    14701 gcctttactg gattctttaa aagaagaaaa aatcaagatt gctcttgctt ctgcaagtaa
    14761 aaacgggcca ttcctacttg aaaaaatggg tcttacacct tactttgatg ctattgccga
    14821 cccggcagaa gcaacaaacg gaaaacctgc gccagatatc ttcctccttg cagcaaaagc
    14881 tgtcggacta aaagcagaag aatgtatcgg gatcgaagac gcgcaaaccg gtatccaagc
    14941 catcctctca agtggcgctc aaccaattgg cgtgggacgt gctgaagacc ttggagagga
    15001 gatcccgctt gtccctgata cgacttttct tacgatcgat tacttgaaag aaaagtggca
    15061 tgaccatgga taatccagag atctccgtcg taccagttcc agagactgaa ataaagaaaa
    15121 tcacattgaa gaacgactac ttagaagtcg ttcttctcaa ttacggtgcc cgcctgcacc
    15181 aaatcttcgc accagacaaa gaaggaaaat cagaaaatgt tttattgtct tacgatcgtt
    15241 tcgaagatgt tctaacagac aaaagctttt ttggagcaac tgtcggacct gtagccggaa
    15301 ggatccggga tgcttcttgg ggtgagcata gattggagaa aaactgcgga tcacaccata
    15361 tccacggtgg cacgaaaggc tggtctttcc aattttggga agtagaagtt ttcaaaactc
    15421 catcatcaat aggtgtggtc ttttacttaa aagatgaatt ttctacctat ccaggaccaa
    15481 tcactgctac gatcacttac cgtttaacaa aaaacgaatt agagatgaca acctctgcta
    15541 gtagccttgt tgaaaccatc tgcaatccga ccaaccatgc ctacttcaac ctttcaggaa
    15601 atggaacaag agacatctat gaacatcagt taacggtctt tttagatggt gtgcttgaat
    15661 tagatcaaga aaaactgcct actggaaatt ggacaaaaaa agaaaaactg ccaatcgact
    15721 ttagaaaatc cccaacactg caagagatcc ttgcatgtta tccagatgga ctagatgatg
    15781 tatttctttt gcatcatcct aggctttcca aaacttcctt acaattattc gagaagcatt
    15841 ctggtcgcca aatgactata gcaacttcta ataaaagtat ggtattattt tctacgactg
    15901 gattcgaagc tgatttctct gtcaatggaa aacagatgca ttcaaactac ggattagcaa
    15961 tcgaaccgca agaaatccct gatatcgttc atttccccaa atttggatcg atcaacctcc
    16021 atcctggtca agaacggatc agtcagacga tttatcggtt ctctgcacaa taacaagaga
    16081 aaggtgtttt tatcatggct attaccgtaa aagatgtggc gaaaaaagcc ggtgttgcca
    16141 cctcgaccgt ctctcgtgtc atcaacgatc atcctagtat ttccgagaca acaaagaaga
    16201 aagtccgaaa aattatggat gaactaggtt atgttcctaa catgactgcc cgcaatctag
    16261 gaaaacgcat ttccagtgcc atcggcgtaa tcttaccccc tcttgattca aaagagcgga
    16321 tcggtaaccc attttatctt gagatcatgg aagcaatcaa cgaagaagcg cggaattatg
    16381 atatgactac agcaatcgct actgctaaaa attttgaatt gctgttagag aacgtccagc
    16441 gtatgcattt acaaaaacaa gtagatggtt ttattctagt gtattcagat agtcaagata
    16501 ctgtcattga ttatttatac caaaaccaca tccctttcac tttgatcgga cagccttatc
    16561 atcatgagaa cgaaatcgtt tatgtagata atgataacca gcttctaggt aaacaagcaa
    16621 cagaattttt gatccagaat ggacataaga atatcctgtt tgtgacaaac acgactcatg
    16681 aaagtttgta tttcgaacga tatttcggtt atcaaaaagc aatgatgatg gctggtcttc
    16741 ctgcgttatc cagtgtagta ctagagcatc cggaggatta tctgtccttt gagaaaacgt
    16801 tgaaagaaaa cgaagccaca gcattggtgg tcattgacga tatcttcgcc cttcgcacga
    16861 tgcagttggc gcaaatgtat ggctatgtca ttccagagac actctccgtc attagcttca
    16921 acaactcgat cttctcaaca ttaacacacc cttaccttac aagtatcgat atcgatatct
    16981 ccgaattagg acgtgtcgct atgcaaaagc tgcgcgaatt gatccatgaa gaattgagca
    17041 gtggcgtacg tttggtcatc cctcatcgct tgatcaaaag agagacagtc gttcctttag
    17101 aaaacccata ctaaatagct gactcactaa atacgctgac catttatcct atggtcagct
    17161 ttttatgtaa aagaagatac tacttctaac acattgttta tctgtaaaaa agagcgtggg
    17221 acaaaagtaa agaacacttt tgtctcacgc tctaaacacg tataaacggc ggaagcagaa
    17281 gcaactcctt cggaaataag ctgaaattca caaaaattta aaaagcaatt ttcttgagtt
    17341 tcttcttatt tcttggagtt aaacgcttct gtcccagcct cttttttcct tccttcaatt
    17401 ttttacacgt cgacgattca ataaaaaacc gcctaatatt gtaaagacag gtgcaagaat
    17461 cgagaaaaag gcgaatggca agtaatgcaa cgcgctaact ttcatcgttc ccataatgaa
    17521 tgtgccgctg actccccacg gtacgagaga attgacggtc gcacctgaat ccgctagtgt
    17581 tcgtgtcaaa taggtttttg ataagccaac gtcatcaaaa gaacgcttga atgtttctcc
    17641 tggaagaatg atggacagat actgttctcc aacgatcaga ttgattccga tacagctcaa
    17701 ggcagtaaac ccgactaaac gagaaggtcg gtccatcttt tcttttaatt cttgaacgat
    17761 ggtatcaacg atcagatatt tgatcagtaa cccacctaat gccaatgcaa gtaaaatcaa
    17821 actagcagaa cctaacatac tcatgatccc gcctcgagaa agcagttcat ctagctttgg
    17881 atcaggtgta tttgcgacat aaccattcat caagatgtct gctgcttttc cgaccgtcaa
    17941 acttttctca tggataaaag aaagaacgag agcagctgtc gatcccgcta gtaatgtagg
    18001 gatagccgga atttttttga ttgccccccc aaataaaata actagtggaa tcaatgtcca
    18061 aggagagatc caaaagctcg ctttcatgtt ttggatcatt tcattgatac tagctagatc
    18121 ggctgaatga ctgttatgtc ctaatatcgt ataaaaaatc aacgaaagga cgaatgctgg
    18181 gaaatccgta taacgcatac cagcgatatg ttcgaataga ttaactcctc caatacccgc
    18241 tgccaagttg gtcgtatctg ataaaggcga gcagttattc cctaaaaagg caccggaaac
    18301 gactgcgcct gtagtcattg catcattgaa tccaagtaaa tgaccgatcc ccaaaaaagc
    18361 aattccaatc gtcgaaatcg ttgtaaatga actgccgaca gtcactccga caattgcaca
    18421 aacaagaaat gctgtcggta aaaagaaacg cacagaaaca agtttaaatc cataaaccat
    18481 aatcgtaggg atggttccag ataaaatcca tgaagcaact aagacaccaa tcaaaagaaa
    18541 aatcaggatt ggaatgatcc ctggagtgat tcctttgatg atcccgtcat ggatctcatc
    18601 ccatgtgaac tttttaaatc tgccataaaa caacaaacac atgaaagctg caaggatcgg
    18661 gatatgtggc gagagctgga atccgatgat cagtactcct aggagtgcca acaacacgat
    18721 caatacagcg atactttctt ttaaagaaat atttttattc atcgtcgatc tcctatttca
    18781 aatgaaaagg ccctgccatg aaaaaactat tcacttggca ggacctccta ctaaatcagc
    18841 gacaaaacgt cggctgaaag acttattttt ttatttttct tctaaacttt tttctacctc
    18901 aatcccccaa gaatctttct tgaagtgatt gatgatcaat ggaataacga acatgaccac
    18961 tgtgatagct gctactaata atgcatcact caatgtcgct gtacctaatg tagcaaatac
    19021 gacaacgatg atagagaaga tcagcatgca gctgactacc caagctaatc cgtttccgtt
    19081 tttaccaatt cggtaaggac gttcgaattc tggatgtttc ttacgcattt taagcaatga
    19141 aacagcaatc aaaatgtaca cgatacaata aataacagtt gtagcatttg taagtgtcaa
    19201 gaatacagca cttacgtcat ccattacacc ataaagcaaa gcaaataatg aaataacgat
    19261 tgattgtgtt agaacgacat ttctagatac gccgtatttg ttttctttat ggaatccgaa
    19321 ttttggtgga aggaatcctt cacgagctac ttggatgatt gtttttgatg gtccagtaac
    19381 ccaagctgat aattgtaaaa gcacaccaat aaataccatg aagctgaaga tatttccgat
    19441 cacttctggt aagcctagga tttgacagtc aatcaagata ggctgcgtga tatttgctaa
    19501 ttccattttt ccatctggta cgacatttgc tacaagcatc gcattgatga cattcaataa
    19561 aactaatccg atcaaagcga taaatacgcc ttttgtataa ttttttgttg cgtctttcaa
    19621 acgaggcatg taaacagaac tcatttcgat cccaacaaaa ataaatgtga taccagctaa
    19681 gtatttcaat gaatcaatat gttccaagtc agggaaagct tttgaccatg aaaagtcacc
    19741 tagatagccg tttgagacta gtccgacttt gaaggctgcc aaaacaccta aaacgaacat
    19801 gatgactact gggatatata cccctagcca tacaccgatg ttcccgccga cttttgccat
    19861 atcgaatttc aagttcaaga tcgtgatgat ccagtagata actaaaatac aacctaaaac
    19921 gaaccagtga ttattgccaa gttctacatt accaaatgtg tttcctaaca atggtcctaa
    19981 agtagaagcg accattacca tacctgggaa catttgcacc cataatagcc atgcaacgac
    20041 aaatccccat ttgctgccca gtgctgtttt tacccataac tgaggaccgc cttcttgagg
    20101 aagcatcgtg gaaagctcac cggaaaccat tgatatcgga ccggcaaaaa acaaaactgc
    20161 aaaaattgaa taaaaaatca atgtccaacc gactgcggct aacgtaggaa tactccgaac
    20221 cgttgcacac agtgccattg tcagaccgat aaaggtccca agacccattt tacttacttc
    20281 agatttactc accgttgaca ctctccttga ttttttggat aaaataacgg tgtctttgaa
    20341 gtagtttcag caataaacga aacaattcag cgaatatttg aggggattct ttgagtttat
    20401 tgcttgaagg gtttcttcaa agacaacctc aaaacaaagg gatcgaagag cttaggtctc
    20461 cccttgctct tcagatccaa acatgcgtca gaaacagtta gtattcaagc tgttttctaa
    20521 catgattacc ttgcttattt tgcttcgctt gccaataatt ggtctgcgta gattttttcc
    20581 aatttttctt gaagagctgc tttgattttt tcagcatatt cttcaaagtg ttcttctttg
    20641 ttcatgtatg gtgtcattac gctggcacgc aatacagtta cttttccggc acggttccat
    20701 tcttcatctg agaatcctag ttggttcaca aattgtaatg ggctgttgcc atagtctggg
    20761 atagcaaagt cagtatgtga agttaagaac tcattaccgt aaatgcttcc tttcacgtat
    20821 gaagaataat catatacgtc atggttcaat ttattcattg caactaagtc atcgttgcct
    20881 ttttctttga atacatagtc taccatattg aaatctggat aagttaatgg atgaacttcg
    20941 atttctttgt ctcctacttt aaatgacaag tcattcaaga agttgtagaa acgatgtgca
    21001 ccttcgattg atgcacccat taatttacca tatcctgtga cattcaaagg taagacatgg
    21061 tgtgctgccc aaacgctggc tgctgttgcc cctgcttttg aaccttccaa gatataagcc
    21121 ccaagtaatg ctgggatatc tgcgcctttt tcaaatacat atgtcgcaaa gtaagagata
    21181 acgtctctca tgcggatatc tttgatgacg ataccaccgg cagaatatgg aacatagccc
    21241 attttatgtg ggtcaatcgt tacagattct gcttcttcga ttgctttata tgcactgtgt
    21301 acttcttcta agatataatc tttgttttct gtaaagacat tgtatttgta atgaacatct
    21361 tttaaatctt caaatggaat gaagttgttg tcttcgtcta agaaaatcgc acggccgtat
    21421 ccgccataag cagcatctac gtgtaagtag aagtaaatac cgtctttttc taatacgcga
    21481 cgcaatgcaa cgattttatc gataccgtcg attgctcctt cttctgttga tcctacaaca
    21541 ccgactacac ctaggattgg tgttttttca gctgctagac cacgaacgat tttttcaagt
    21601 tcgttgatgt ccatgcgata gttgtggtca actggcactg gaatcacttg gtctaaaccg
    21661 ataccgatga tgtcggcagc ttttagccat gaatagtgtt tagtttgtgg aaccaaccat
    21721 tttcctaatt tttctaaatg ttttccgcta cgtgctgagt gagctttgat ttcgtcgatt
    21781 ttttctggga cgctgtctaa cagattcatg atttcttctg ttgacaagtt cattaattcc
    21841 caatcgcttt ttccagcaac tagttcaggt gtcacttctt tcattgcaag cggcaatgat
    21901 ttgatgttgc gggcatacca aagtccttct aagtttgcta aagaaccatc agcaacgatg
    21961 tggccccagc catctttata gctcattaat tttgcaaatt ccattcctac ttcttcttcc
    22021 atttggcttg ttgcaggaga agattcatag gcaacgttat taccattcca tagcatagca
    22081 aaattgtacg ctagtaaaga tggcatcaat gtttcagagt tcatatgacc ccagtaacgt
    22141 ccggcattat gccaaggaac ggaatgggta cgcatccgcg ctgaaatttc tgatagaaca
    22201 tcctttgttt tattcacagt gtgttcaaaa ctagcactgc ttttttcttc tggcgtaata
    22261 ataggcatgt cttgaggcat gtagttttga cgccagccta aatgttcatc caccaattca
    22321 ttcaacaaag ctttatagat ttggccgttc tcagctttgt ccccaatgaa tagggcattt
    22381 aggtttagat ctttcgacaa tgattcactc atagtgaaac cctcctaaaa aaatatttaa
    22441 attttgtcga gccctacatt acacctgttc ttctattctg taaactaagt tttggcctga
    22501 tttttaatca tattttaata tgtatcaatt atccgaattg ttcacacttt tgaaataatt
    22561 aaaaaaaacg agggaattta caaatgtaaa ctccctcgca aagattgtat taacgacaac
    22621 cagcgttcta ttttaggatg ggtttcaaaa ttggaatgtt gtaatagtat ttcccttttt
    22681 caaggtaaaa acttactaaa ttctaatcaa ttactttggc caaaaagtaa tttttcttac
    22741 cttttcgaat aaccacaaac ttaccatcga attcatcgga tggatttatg acaaaattta
    22801 aatcggtaac gcggtcaccg ttaatactta tagctccgtt tgaaacatct tcacgtgctt
    22861 gacgtttaga tggttcaatt tttgtggaaa caagcagttc cacgatattt tcaggtgtgc
    22921 ttgaaatttc aacgttcggc attttaccga atccttgcgc gatttcttcc gcatttagtt
    22981 ctttaatatt tccagagaac aatgcttgcg ttattttttg cgcttctttc aagtcttctt
    23041 cactatgcac aaatcttgta acttcttctg ccaaacggcg ttgtgcttca cgtttctcag
    23101 gttctgtctc tacttttttc gctaagtctt cgatttcttc ttgagacaag aaagtaaaga
    23161 atttcaagta tttcactaca tcgcgatcat cttgattcaa ccagaattgg tagaactcaa
    23221 atggtgaagt tttcttcgga tcaagccaaa cggcaccgcc tgcagttttt ccgaatttcg
    23281 ttccgtcagc cttcagcatc aatgggatcg ttaaaccgaa cgcttctgct tctggtcctt
    23341 ctaattttcg gattaggtct aatccagctg tgatatttcc ccattggtct gctccaccga
    23401 tttgcaattg cacatcgtat gttttatgca gatgcaaaaa gtcgatagat tgcaggattt
    23461 ggtaagtaaa ctctgtaaat gagattccta cttccaaacg gctggcaacg atatcttttg
    23521 ctaacatcgt gttgatgttg aagtttttac cgtagtctct caaaaattca agtaaagaga
    23581 tttttgacaa ccagtcgtag ttgttcacaa atgtgatatt tgcatcttta ccaaacaatt
    23641 ttctcatttg attggaaagt gcgtccacgt tatgttgcac ttgttccatc gtttgcaaca
    23701 cacgttctgt tttgcggccg cttgggtcgc cgatcgttcc tgttccgccg ccaatcagaa
    23761 tatatggatg atggcctgcc aattggaatc ttttcatcat cataaatgga atcaaatgtc
    23821 cgatatgcat cgaatcacca gttggatcaa cgccgcaata aagtgaaatg ctcttttctt
    23881 caacgagctc gcgaagtctt tcttcgtttg tttgctgatt aatggcgtcg cgccaagtta
    23941 attcatcaat aatattcatt ttactgacct ccgtgtagtt tttttatgaa agcaggaacc
    24001 tgctagcata tgtaattaat tatttcttca ggataggata ggaaataaaa aaagtccctt
    24061 ggcatataag ccaagggacg aaatctcgcg gtaccaccca atttgtatgt ctagatatcc
    24121 aacatacctc tcgaacattt aacgctgtcg cgtggcaatc actgccaaag actcctgaaa
    24181 tgtaattcgc tttaaagcat gtactggttt gcagcgacca ccagctttct gaaacattgg
    24241 ctttaacaac tacttcgttt cgatcttcgt cttgttatta ttaatatgtg tctatcatag
    24301 agcgcaactt tttctttgtc aacagaaaaa ggatattttt tttaaattcc tcattttttc
    24361 ttttttgaat ccgctctaat tccgaacatt ttattttcaa ttattgttaa aagaaataac
    24421 tttttctgta ttttttctgc aatttatcga gaagtcactt tactttgcct cttattcttt
    24481 cttcgttaca ataaggaatg ggacaagtaa aaaaatgaaa aagcaaggga cgaggtatat
    24541 ggaaaaaaag caaaaaatta ttttaggagc cacttctgct ttaactatcg ttattttaat
    24601 tctaagtttg ttttttgtca tccgatcatc tacaaaaaaa gaaccaatca ttcctgaaaa
    24661 aaaaataaca gaaaaagcaa aagaaccgac agatgataag caacaactag ttactcaagc
    24721 aaaaaatctg gctcaaagct acgattatga ccaagcgatt gcgttattgg aaaaagatag
    24781 taagaacgga tctactgaaa cacaacagct tcttcaaaca ctaaaaaagg agaaagaaca
    24841 actgatcgac tggtctgatc ctactcagat ctctcatgtc tttttccaca gcttgatcgt
    24901 ggacccgcaa aaagccttcc actctcaaca ggcacaagga tacaaagatt atatggtgac
    24961 tgttgaagaa ttcaaccgtt cgatcgaaca actttatcaa aatgattttg ttttagttaa
    25021 tttgaatgat ttgatccaaa aagacgaaca aggaaatctt tcctttaacg ggcttgcgct
    25081 acctgaagga aaaaaaccat tggttctttc acaagatgat gtcagctatt atgaatatat
    25141 ggataattcc ggttttccta gccgattggt cgtatcaaat aacaatgaaa tcaaaaatgt
    25201 ctataaagag aaagataaag aagaaatcgg tgactttgac atggttcctc tgatcgatgc
    25261 ctttgtcaag aaacatcctg atttttctta tcgaggcgca aaaggtacct tggccctgac
    25321 aggttacaac ggagttttag gttatcgaac atcaaaatct caatatggag acaatgaaaa
    25381 aacaaaaaaa gagattaaag aagctaaaaa agtggcggaa cagttgaaga aagacggctg
    25441 gagctttgct tcccatacat ggggtcactt gaatatgact caagcttcac tagaagatgt
    25501 aaaaaaagac aatgaattgt ggcagcaaga agttgcacct atcctaggaa aaacaaatat
    25561 tctgatttat ccatttggtg cagacatcag cgactggcag ccttattcag cagaaaatca
    25621 aaaatttgct tacttgaaga gtcaaggttt cgatattttc tgtaatgtcg atgcttctac
    25681 accagcttgg ggacaattag gtacggattt ctatcgaaat gctcggatca atatcgatgg
    25741 cattcgtttt caatctgatt tagacggaaa aaatcctatt cttgatccat tcatcaatgt
    25801 ccatgatgtc tatgacaaag aagcacgcga atctgcttga ttttacgtca tttatgaacc
    25861 aaaaaagccg aactgacatt ttatcgtcag tttcggcttt tttatgcttt atttcttctt
    25921 cggtgcgatt cccttgatca ggacttctgt ccattctgct gtatatggaa gcagttttgc
    25981 gtatatctcc ggcttagaaa ttccttctac cattgcttga aaatagagga tcaagaactc
    26041 atccgaatat ttcaaatcca cttttccttc tttccgccct cgatggaaaa gatcaagcat
    26101 gatactgaaa ctttcttgcg tatacgcatc catcgcgtca gaaaattctt ttttttcttt
    26161 ttttgtatag tatgtcatca aatcgatata aaattgtttg ttcactcgat tgagataacg
    26221 gattttgttt tcactcatgg caatcagtgt ttcttcaaat ggaagatttt ccgcaatcaa
    26281 cgctttcgct tcttgcccta aatcattcat aaatgcaaaa aatacttcat aaatcagccc
    26341 ttccttgctg ccaaaatatt taaaaatcgt cgctttactt acctgagcgt tctcagctac
    26401 cttatccatt gtcactccag cggttcctat gtctgtgttc atcaagtcaa aagctgcttg
    26461 taaaatctct ttctttttct cttgtgctct cttttcaaaa ccgttcattt gtagagcctc
    26521 ccgtaatttt aaacttttat aatatatata gttcaaattt attctgaggt ttattataac
    26581 tatctattga gaaagtatca agctagcaat tccttgttta tgaattcatt gacagaaatt
    26641 tataaaaaga atattatgta ctaaaataat aaaaaaagtt ttataaaaag gtataaaacc
    26701 tttttattaa actatttaat cttgtttagt atatattttt agaaaaagga gtggggtttt
    26761 tatggcagaa atcgtcaaag tcacacattt gaaaaagagc tttggtcgtt tcgaagcttt
    26821 aaaagatgtc tctttcaccg tacattcagg tgaagtcgtt ggatttatcg gaccaaatgg
    26881 ttctggaaaa tccacgacga ttcggacact tttagggatt ataaaaaaag acggcggaac
    26941 ggcaacgatt tttgggcaag atgtatggga agatagttta tctatccatg aacgtctttc
    27001 ttatgttcca ggcgatatct ctttgtgggg aaatttgact ggaggagcga tcatcgatct
    27061 cttcatgaag cttcatggaa aagggagtgc tcaaaaaaaa gatgaactga ttcaacgttt
    27121 ctctcttgat cctaagaaaa aagcaaaaaa ctattcaaaa ggaaatcgcc aaaaagtggg
    27181 cttgattgct gctttggctg ttgattcgga tctctattta tttgatgagc ctacttctgg
    27241 tttagatcct ttgatggaaa cgattttcca agaagaagtt gaaaaattga aagaacaggg
    27301 caaatccatc ctactttctt ctcatattct cagtgaagtc gagcgactag cggataaagt
    27361 cgtgatcata cgcgaaggaa cactcgatga agaaggaaca ctcgatgaat tgcgtcattt
    27421 gactcgttcg accatcacta tagaaacaga agcagagaat agtcacttag cacaattgaa
    27481 cggtgtccat gactttgtgc aaaaaggtca cctcgctcaa tttgctgctg ataacgaagc
    27541 tttcgatgtg attttagctg aagcgtccaa attaggtgtg aaaaaatttg aatctattcc
    27601 accgactctt gaggatttat tcatgagaca ttatgaagga tgaggaggaa gcgaaatgac
    27661 tgataaattt tcacgctggt ccaccctttt ttctcaaaac cttagccgtg actggaaaaa
    27721 gatcctgata tggctagtcg gtgtcactgc tttttctggt ggttttgtcc ctgcgtttga
    27781 agaattgact tccggacaag gattagctgg attatacgaa accttgcaaa acccggcaat
    27841 gattgcaatg gtcggaccaa ctccagcttc gaatgctgct gagtatactt taggagcgat
    27901 gtatgcaaat gaaatgttgc ttttttgcag tttgtttgct gctgtgatct ctgttttaca
    27961 cgtggtgagc caaacgagaa aaaaagaaga acttggtctg attgaactta ttcgttcctt
    28021 tcgagtcgga cgtcaagcaa actctttagc tgtcatcgct gaaagtattc tgatcaattt
    28081 gcttttagca atatcgatca gctgtacgat gatttttttc caagcggatt cgatcactgc
    28141 agaaggtgct tggctttttg ggctatctgt aggtagtatg gggattttgg ggagtgccgt
    28201 tgcactgttt gctgcacaac tgatgccaag ttcagccagt tcgaatggtc tgtctttagc
    28261 gtttttaggt ggactttacc ttctacgagc aataacagat gtagctgatt cctccctttc
    28321 gatgtggaat cctttaggct ggtcttattt gacctatcca tttacaacta acaactggat
    28381 tcctttgctt tatttgtttt tgttcagtct aatcttgata atcagttcat ttgttttaga
    28441 ggaacatcga gatttgaaca ccggctatat ccccgaacgt gaaggaaaac cgtatgctaa
    28501 acgttctctt ttatccttgc gtggtttatt attctatcta aataaaggga tgacaatcag
    28561 ttggcttatc acttttggtt tgatgggggc ggcatacggc tctatctacg ggaatatgca
    28621 acagttttta gaaagtaacg acttaatgaa acagatgttt atccaatctg gcgtctcaat
    28681 tgaaaaaaac tttacaagca cgatcatggt agtcttgatt tgtctagctg ctattttacc
    28741 aattgctagc atcaatcgtt tatttacaga agaaacccac ggacgtatga atcagctgtt
    28801 cgcaacaaaa atttctagga accaacttta ttggaccaat atgttactag ctattctatt
    28861 tgctatattg gcaatcctga ttagttctgg tgcactaggg ctttctgctt tggcttcgat
    28921 gaaggatgcg aaagacatgg atctattgat gtttcttaaa gccgggttca atttcctgcc
    28981 tgccgtttta gtgtttatcg gaattagtgg ctttctattc ggatggcttc ctcgaatcgg
    29041 taaacttgct tatatctact tagcttactc ctttgcactc aattactttg gcggaatttt
    29101 ggatttacca gaatggtttt ccaaaacagc tgtccctagc tgggttccac ggatgccaaa
    29161 cgattctttt gatctttccg tgttccttac gttatcatgt atcagccttg cactgttcgt
    29221 ccttggttct attggttata ggcgaagaga cttactagaa ggataataaa aaagcatgta
    29281 caatttcatg ctagcctcca aatctgggtc aaaaatccaa catttggagg tcattttttt
    29341 atttacaagt cttctaactg ctctttatca atgatccaga cttctcgatt ctttcttttt
    29401 acataaccat tctcctctaa taatttaaat tttctagata aagtttcggg ggtcgtaccg
    29461 ataaatgcag caagttcttt cattttcatt ggcaggaaaa agtgatcctt ccctgtcgct
    29521 ttagctaagt caagcagata gacagcgact cgttcttcta ctttttccat catcaaaaaa
    29581 gttgtctgct gctgtacttt tgttaatttc tcaacgttga ttgctaaaag tttcagacta
    29641 agggcgggat aatgcagtaa gagttccgag aaatcttttt gtctcaatat gcaaatttcc
    29701 gttttttcta gtgcttcgcc aaataaagac tcatttggaa cgcctagcaa ctggctctct
    29761 ccctcatatc cgcccggctc tattactcgt aaaagctgtt cctttccgct agcagataat
    29821 tgatacactt tcatactgcc ggaggccacg atcactaatt gaggttctcc attcggcgca
    29881 agaatctgtt ctcctttttc cacatagtga tgtttgacaa gtgcgtgtac tttttcttga
    29941 tcttgctgta ctaagtgatt gaacaaagga acaaatgaaa cacataaatg atcatgactc
    30001 ataatctctc ctccttctaa acagctattt ctaataaaga aacataactt cccttattcc
    30061 aactgataat tttcttttat catagcataa acgtcaagcg aaccagatac cagcaaatag
    30121 agtggatttc tttttgtccc ttgctttatt ttccttcttt tgagacctta agtaattgat
    30181 tcctatcaat tttttaaata tttacttttt gaataatcaa aaacaaacct tcactcgcaa
    30241 gccgagagta aggtttgttt ttcgcattgc ttatttcgtg ttgtctattg aaatttttcc
    30301 gcacctagct tttgatatag tggtgctagt tcttgtaagg tttcagcggt tatttgctca
    30361 acgtgttcga tttcttgcgg atagaagaat agtttaccca tttgaaaggt tcggtctctc
    30421 atcgcttttt tcaactcttc ttgtttcttt gtatcggaca aatagtcaga caaagctaag
    30481 tggtcgtcaa gttcatgttc tggttgcggc catatgtaat aatccttgat atcgactgtt
    30541 ttgctccact ctttcagtat cgcaagcaac tggttatacg ctgcaatgct accatatcgt
    30601 tcttcgcttt tataatgttg gtacatcaaa tagatttggt attgtttttg attcaacaaa
    30661 actgctagac aggcattctc gtcttgacga gattcgcttc ggtacgcgct ccaaaaatga
    30721 ctgcgtaaat tccagccatt cgtccagctt tcgattttag gtttgctcat gccgtacata
    30781 tcaggcaaaa gtgcagctgt ctggagctga atctctttcc atttttgcca gtgttcttta
    30841 tattctttct tgatttgttc aatcttatct gttggatggc tctctttgag ctgtttgaat
    30901 gcgtagattg gtcgatcaaa caactgataa tctgattttc caagttccat tgtttcgcct
    30961 ctttccgtta tcttttgtat tctacctgaa ttttgcgata tggacaaata gaggttgtga
    31021 aaaagctgtt ctgcatcaag tcataagaac agccaaacaa aaaactgcga tgagcatcac
    31081 cgcagtttta ttgaacttaa ggttgattga tcacatctaa aagcgttgaa aataaaatgg
    31141 cactagaagc agctcctggg tcgatatgtc cgattgcacg ctctcctaga taggaagctc
    31201 gtcctttttt agctaccaag actttggtgt gttcttttgc ctggtcaatg atttcttttg
    31261 ttaaattccc tgccttcaat gcctctgtga ctggcagcca aacatctaac atcgttttat
    31321 cgtcagcttc tgcctttcct cggctttgaa tacccgctgt tccctgctcg atcacttcgc
    31381 ctaatttttc ttgagaatca atagaatcga cctcttttgt cgctttcatc atgttcatga
    31441 atgcagatcc gtataacgga ccagaagccc caccgacttt cgatatcatc gccatagaga
    31501 atactttgaa tgtatcgctg attgtttcag gatgatcggc ttgaaaagct tcttcataag
    31561 ctgaaacccc tcttcccatg ttatttccat ggtctccatc gccaatcggg gtatctagat
    31621 cactgagata ttgtttgttc tgattgattg cctctgcaaa ttgcgtgagc cattcttgga
    31681 tttcttgaac ggttagtttc atattatcac ccttttatct tgagattttt taccatgaaa
    31741 tcgtctgtac attgctttct aaagaatctt tccattgtgt ctccgtcaag tcgatcagtg
    31801 ttagagaaag accttgcata tctagtgagg tcataaaatt accgactttt cggaaaacta
    31861 cattgacgcc tctttcttcc aacagagcta aaacatcatt catgaacacg aactgttcca
    31921 tcaaaggcgt cccgcccatt ccgttgacta gtacacctgc ttcttttggt aaattcgagt
    31981 aatcatctaa aatcttagta acaagttcct ttgccagtac tttagaaggc tgcatctttt
    32041 ctcgacgata gcctggctca ccgtggatac caacaccata ctcgatctca tcttctccta
    32101 gttcgaatcc tggtttccct acttctggta cagttgctgc tcgaagcgct agaccgatcg
    32161 tcttagttgc ctgtacgacc ttttctccca gttctttcaa ctcagctagg gaagcaccgt
    32221 ttcttgctgc atcgcccacg attttgtgta caaggacagt acctgcaact ccgcgttttc
    32281 cagcagtata cgtactattt tcaacggcga tatcatcatc tacgatcaca ctttctactt
    32341 caatatcgtc catagcagct aattcctttg ccatatcgaa gttcaatgca tcccctgtat
    32401 aattcttaac gatcagcaaa actccttttc cactatcggc ttccttaata gccgtctgaa
    32461 tctgatctgg agttggagaa gtaaacacat caccaagcac cgctgcactt aacatccctt
    32521 cccctacaaa cccggcatgg gatggttcat gtccgcttcc tccaccagaa accaaaccaa
    32581 cttgttccgc tttctgattc ttggctacaa cacgggaaga ttctacacga tgaacaagtt
    32641 ctggatagct ttttactaat ccttccagca tttcttcgac aatattgctt ggatcattca
    32701 tgattttttt caatttattc ccatcctttc tttccggcat tttgtgcttc tgttaagatt
    32761 tgctgagtat catctgttat accggcagtg atggctgctg caaaagctcc ttccactaaa
    32821 ggcgcatcca ccagatgata acgttgctgc tgctcttctt ccaacatgtc ttttgccagt
    32881 tccgagttca ataccgcgct accgatatcc gcaaaaacaa aataactagc tgcatcgctt
    32941 gattcattga ctgcttctac gattttcatc gggtctgatc cgatctcgcc atttattccg
    33001 ccgagagaaa aaatttgcac ctcttcggat ttttgcatct gctcaatcat ttctttaata
    33061 ccatctgtta ctttttgact atgagaaata agcaaaatac tttttttcac aagaatcttc
    33121 ctttctttta catagttgct aacgattggt ttcgctttca ttaatgaaca attcaagtat
    33181 ataataaatt tctaattttg ggaaaatatg tgtagtgctt cttgaagttt gttatacttt
    33241 aattaaaaga gaggagtgat cgaatgatta caacaacaac gaacagtgta gagggaaaag
    33301 ccgtagtagc ttataaaggg atcgtttttg gtgaggtcat tactgggatc catgcattta
    33361 aagacttgga ggcggggttg cgaaatatct ttggcggccg ctcaaaaagc tatgaaaacg
    33421 aactaatggg tgcaagagaa gaagcacttg ccgaaatggc cgaacgtgca aaagcattag
    33481 gagctgatgc gatcattggc gtaaaaatgg actatgaagt attaggcagc gacaatggca
    33541 tgctgatggt cacatgtagt ggaacagctg tctccacgag ataagtaggg gacatctgtt
    33601 ttaataacta aagagagatg ggacatgtag tggcactcaa gaattagatt tttcagtcta
    33661 acttcgaggt actctacctt cccatcctct cttttttatt tctttttgcg gatgattccg
    33721 taagctgctg cgactaacac acccgcccct gctaaggaat aactgacaga agctgttttt
    33781 tcatttgttt ttggcaaagt ctgacttgtt gatttggatg gctctgtcgt ttgctgaact
    33841 gtttctgtcg tgcttccaga ggtcactgat ttttctcctg ccgaatgcaa aatatctttt
    33901 attcgttgga aactagctgc taagtcagct ttttttgctt caaaatcatt agcaactgtt
    33961 tgtgcttcgc tgatctttcc gtggattgct tgactggccg ctgtgatatc cgcttgagtt
    34021 tgacttagct ttccagaagc tgtttgagca acctgattta attgctgatt gaattctttt
    34081 gcttgttgga tcttcccgtc aagatctgtt tggacagctt ccgcttttgc tttcgcttcg
    34141 gcaagctttt tctcaagttg tgcacggtca tttcccactt tatttttaag atcagctaaa
    34201 tcttgatcga ttgctgcttg gtcttctttc aatttgtttt ttgcagcttc taatgcttct
    34261 tcgagttctt ttcttttatt ttccaagtct tgcaaatgat cgatttggtc agaagaggta
    34321 gaactattta ttgatgtatc ttctgaagat gaaatcgttt ctcctgaatt atctgtcggt
    34381 acattggaag tcatttctgt ttccgaggca ccgttttcag caccagaaga gcttatggtt
    34441 gttgaaggtt ctgtttgagc aggttcagaa gtttccgcaa aagctgtctg tcccaataag
    34501 aaaatactgg aaacagcaat tggccagatt aacggcttca ttcttttttt catcactttt
    34561 cccatcctta ctacttttta actaatttct tattataagc aaaaatgttt ggaagaacca
    34621 taaacacaac aggctgtttg tctaaaaaac ggtgcatctt ttattttttt gtacgttccg
    34681 ggcttttcca tgtcccgctt ccataaccta aaatcgtttt gactgctgga atgaaagtcg
    34741 tcacgatcgt tatcgcgttc actatccaga acaaaagcat atatagtggt gcgaaaagaa
    34801 ggtatttcaa ttttcttcgt cgatcatcta caatcagcga agctagcaac tggaaaaatc
    34861 ctgcgaccat ttcaaagcag atgaacacaa aactgaatgt aaaagtaata taaatctgtt
    34921 cgtagtttcc ttgatataca taatgcccga ttaatccagc aaaaagaact acggaaatac
    34981 aaaagaatat tgaccagaca atactcaatg tttgatcaat gaagatgatc gttcttccaa
    35041 tatgtttaaa aggatggagc atcacttttt tgaaattcgt cagccagact tctgttccac
    35101 cttttgccca acgttttctg tgtcgatata gcatcttcaa cgtcacaggt acttccatga
    35161 aaaagataat ctcaggagca aaaacagata accagtcatt taactgatga tcccaagcaa
    35221 tactgatgtc ttctgtcgct cgatcttgtc tgaatccgcc cacatcgatc aaggcttctt
    35281 ttcgatacat cgtatttgct ccactgtacg cataaagacc accgaaaaca gctgactgtg
    35341 ttcgtttaat gatgcccacg atacttgaga attcaaccgt ttgagatttt gcaatcaatt
    35401 tggtacgatt ttggacatcc atatttgcag tgaccgcagc aatatgtctt gcaccagggc
    35461 gaatgaaata attcacatac ctaatcagtg catctggctc cggtaccgtg tctgcatcat
    35521 tgcttaaaat caatttccct tttgcaaagg caagtccgat attaaacgcg tgggcttttc
    35581 ctttattttt ttcgatccga accacacgaa gattagcgta ttttttcatc agtctagcta
    35641 atatttctgg tgtttggtcc gttgaaccat cgtctgtcac taatacctca tagttgtgat
    35701 aattcaattt cgtcatcaaa tactcgattg tatattcgat cacaatttct tcattatggg
    35761 ctggcaccat gatcgtgatg aacggttcga cactcaaggg aacatctacc cattcttttt
    35821 gcttatgttt gtataaaaac acataacacc agacaccaat aaacgataca aatccgccta
    35881 aaatcggata agaaacgaga aaaaaataca taatagacaa acaagtatct aagaataaat
    35941 tacctgtatg ggtgattgag atcatttcag ttccacccca ttttctttat acaaatctgc
    36001 aatgaatgtc gtatctagat ttttctcttc ggagacagaa taaaaacgga ccgtttctct
    36061 ctcttctttt gttccaaaac gttccgtata cacttcttca agtaattctt gtcttttttt
    36121 caatttttca tcttcatatt caaaagattt atttactttt ttgtatagat ttcgattgtt
    36181 ccacctagtc aatacaaatg aggtaatcag gaaactgaaa aaaatcaata caaaaaagcc
    36241 gattaagaaa tacagcattt tcttttctga taaaaaagcg tacaaggaaa aatccttggc
    36301 tctttctggt ctcgttaatg ggaaaaagac ccatataaaa ggaagaacaa cgatcatcca
    36361 tcctaaaaga gccactaacg tttgccaaat ctttaaccag taattacctt ttgaaaaata
    36421 cttatctttc accaaaattg atgcaacttc ttctcttgtt acttttttca tatcctcacc
    36481 tcacgttttt ttgttacgac gtaggagttt accatacatt tcatttcttt tcacataaag
    36541 tgttcctaaa tttttcctaa gaaaaagaac aaaacatttc tcatcagcaa atgaaaaata
    36601 ttttgttctt tttatcagtt gttcttttta ttttttatct cttaattatg ttgctctttc
    36661 caatacgaca atgcttttca ttcctctaga tatcgccgtg tacatttgtc tgcgtcttct
    36721 ttgctcatct ccatcgtttt caaatggata taagattaca tgatcaaatt ccagtccttt
    36781 tgctagactc aaaggaatga tttggcagtt ttctaatcca tatgaatttt ttttaatcga
    36841 ttcttgtagc gtttgggttt cctcttctgt ttgggtgata actgccgctt tttctccttt
    36901 tagagaaacc aaaatctcag ctagtatttt caacaattct tcagaggatc gaaaagagcg
    36961 gtattcgggt tcttttcctt gcggtcggat cgatacgatg tcgatcttct cttgatccac
    37021 agcataggtt ttgaaaagct ctgtgattgc tccgctagaa cggtaactat ttctaagatc
    37081 ataccgatgg atcggcagat ggtagtcgtc gaaacaacgc ataatatcag caaaagtagt
    37141 ggaagaatta aagattgcct gattctcgtc acctactaaa gtaaagtgag tttttggaaa
    37201 taattcgatc aatagaccta attgtgccag ggtataatct tgtacttcat caatcaatag
    37261 atagcgaaat gctggtacat ttaccttttc aacaagtaaa tgacgaatag ctaatataat
    37321 gactgcttca tcaacagtac gtgtgggttg ccaagcataa gctgagccat atcgtttttc
    37381 ataaattctt tcaaacaact gatgctcatc cacccatgcc atttcttcga tttgtctggt
    37441 tatttttcga tatttttttt gtaaaagttt ccttgcataa gctacaatac tttgttccga
    37501 atcatcagaa atcaatttac cgaataattc caactgccta tcttctgtca acgagagtac
    37561 ctgattctgc acagcaggac tttttgcttg tttcaaaaga tgattcttcc attcgctcat
    37621 cagtgccttt ttcgttgctt gaagcctatc gatcaattta ggatgagcag gaactttctc
    37681 atatattttt tcgatcgttt ttttagaaag gactgttttt ccatttttag taagtccacg
    37741 gataaaattc ggatgatcta aaaacaaagg atctgattgt tttagattat cgataaactt
    37801 tttgctacgc aaacgttctg tctgctccga tacattttct cccgtgatac gtttgaaata
    37861 agcctcttca ccttctattt cctctgctga aagctgtgaa accagctgta tgatggttag
    37921 attcagtgga tttctttcac ctaatgatgg taggacattc gaaatatagt cgataaaccg
    37981 atgattaggt gatagaatca gcaagtcatc ggatgtcatc gtatctcgat ataaatagag
    38041 cagatacgca atacgctgca tgatagcaga ggttttacca cttcctgcta cgccatttac
    38101 caagatattt tgacttttca catcccgaat gattgtgttt tgttccgatt ggatcgtagc
    38161 agtgatagcc ttcatttcat tggtctcatc ttggcctaag acttctaata atacgtcatc
    38221 ttgaatagct acagacgtat caaaaaactt gaccaaacga tctctttcca aaagaaattg
    38281 tcgtctttct ttaataaccg tatcgatttt attttgacga accgtataag aagatggacc
    38341 aatcatgtta ttgtaaaaaa gttcggcaat tggtgaacgc caatcataaa caagattttc
    38401 atctgcatca tctgtgaaat ttgctgtacc gatataaaag gattcttttg gctcattatc
    38461 cagaaagtct acaacgactt ttccaaagta gggacttttc aataaacgtt cgatctttgc
    38521 tagctgattt tctgcgtttt tctgtttgat attcatctga tcgatttctc tatttttcat
    38581 ttcaatcatc gaaaaagtat ccaggttatc taagtaactg tcaaaattca atcgaagctc
    38641 gccagccatt tctttcaacg actggtttcc ttctattttt gccgtatcca tcgattcccg
    38701 cagttcgttt tttttatcaa ttagcttttg atacacgttc ttcagatatg cttcttctaa
    38761 ctgtttttgt gtgttgacca tacacattct cctctttttt taaagtggtg gaaccgatta
    38821 gtttacctct tttaagagtc gaatgcaagc tttttaacga gtatcatttg ttaggatata
    38881 tcagttcttc aggtcggaca gtttgatata aagaaaatgc accatcaagg ttttttacgt
    38941 ggtagccaga ctgcttcaag attctttccg ctagatagct acgtaatccg ctatgacagc
    39001 tgacgatata ttcttgttgg ctgtctaatt catttaatcg gtcccgaagt tcattcaaag
    39061 ggatatgttt ggcttccttg aatttgccct tttccaattc ttctgctgtt cgcacatcta
    39121 ataaaatttt tccttttgct agttctgtcg gcaattcatg ccattgtacg gtttcactaa
    39181 gcccttctgc aatgttcatt gctgcatatc ctagcatgtt tactggatct ttggctgagc
    39241 caaatggcgg tgcatacgtc aattctaatt ccggtaaatc gaagatcgtc aaatgccctt
    39301 tgattgctgt tgctaaaata tcgatccgtt tatccacacc ttttgcccca acaccttgtg
    39361 caccataaat ctcgccagtc tctggatgga agatcaactt cagtaaaata tctgttgctt
    39421 ctggataata gcttgcatga tcttttcctg atacatgaat gacttcaaaa gacagttcgt
    39481 tttctttggc tgttctttca cttaaaccgg tggatgcggc agtcattcca aacgttcgta
    39541 taattgctgt tccaataccg ccttgatttc ttcgagaaat tccggaaatc gtgtccgcaa
    39601 cttgtctacc ttgacgattg gctggtgaag caagagaaat aagtgcatct tttccagtga
    39661 tttgctgttt tacaacgata gcatccccca ccgcataaat atcaggaata ttcgtttgat
    39721 aacgttcgtc tactaggata cccccgcgta tgcctaattc gattccagcg gtcactgcta
    39781 gctggttatc aggacgaaca ccaatggata aaataactaa atcagcaggg atggtacgcc
    39841 catcatccag tcgcaatcgt ttcccgtggt cttcaaatcc agcgacagca tgagaagtaa
    39901 tcacttctac tccttgatga gacaattctt tttctataaa agctgccatt tcttcatcca
    39961 gtgttggaag aatatgtggt gcttgttcga tcaccatgac ttctaaacct cttcttttta
    40021 ggttttctgc catttccaat ccgatgaatc ctgcaccaac gatcacggca cgctttggct
    40081 gtttttctaa tgcatgcatc actttatcga tatctggtac atttctaata gaaaaaacat
    40141 tgtcggcttc tgttatccct ggaatcgatg gaacaactgg ttttgcacca ggagataaaa
    40201 ccaatgcatc gtactgttca atgtgttttt gaccctcatg tacgacttct accgttttgt
    40261 tttcgggaaa gatggctgtc acttcatgat ttggacgcac atctaaacaa aaacgagcgg
    40321 ataatgattc tggcgtttgg acaagaagat tttcccgttc actgatttct cccgaaagat
    40381 aataaggcaa gccgcaattt gcaaaagaaa catacggtcc tttttcaaaa acaacgattt
    40441 ctgcatcttc cattaatcgg cgaagccgtg tagcagcaga cattccacct gctacacctc
    40501 cgacaataac gattttcatc ttatttccct cctactgttc gatcaaacca ttgattcatg
    40561 ccaccacgta cattcactac atcataacca gatttcttta attctttagc tgcttgttta
    40621 ctcctcatcc ctgattgaca aatcacatag acggtctttt ctttgtctcc atcatatctg
    40681 tttatacttt gtaatggaac atttttagct ccttttatat gcccgccccg gtattctgca
    40741 ggtgttctaa catcaagtaa cgctggattt tccaaaagct tttcctttaa ttctgggact
    40801 gttatcgatt ttattgattg aaatagcaaa ttcattttct gcacctcatt tacattttta
    40861 taaatacctc atggggtata ttaaacagga gctttattaa tatgtcaacc agaatgactc
    40921 ttcatttttt tcactacttt ttatatttgc ataaaatacc gataaggggt attatctaat
    40981 tataaaaata gaggagaatg tgttatggag attgataata aaaatatcct gaaccgtttg
    41041 aaacgtacag agggacaaat tcgtggtatc caaaaaatga tcgaagacga aaaagaatgt
    41101 atggatgtca tcactcaatt aagcgctgtt cgttctagta ttgatcgcgt aatgggcatg
    41161 atcgttgcag ataatctgaa aaattgcttt gagaaacctg atagtaatcc agaagaacaa
    41221 gcggctaaac tagaacaagc gatcaagatg attattaaaa agtaaaggaa tgatttcaat
    41281 gaaagtcgtt ttccatattg atgaattaga aaaatggtca gaaacaggga aaaacgtaaa
    41341 aaacctgata aaagcttctc cagaaacgac gattgtcgta tctgtaaatg gcatcgcgat
    41401 taccggttat ctagactctg ccaatgcaga attcttagat ttgcaaggag tcgtttttca
    41461 cgcttgcgcc aatgctatgc gtgctaatca tatttcagaa agttctttac ctgaacaagt
    41521 aatcgttgta ccagcaggtg tccttgattt agttgaactg caatcccaag gttatgctta
    41581 tatcaagcct tagtcagtag aggttgcgac agaagtgatc agcttcaaga aatttcggct
    41641 tatttttcga agaagctact tctggaaaac cgtgaatcaa aggttgtgaa aacgctgtcc
    41701 tgcatcaagt aataagaaca gccaaacaaa aatagcttct catatttttc gtttggttgg
    41761 gcttattacc gcagatgcaa gcttttgaac accgtttaag gaagaggttg tgaaatcagc
    41821 ggtttgacct aagtattaga gaaaaaaacg ggaaaatagc tcctcatatt ttgccgtttt
    41881 ttgagctaat accgaaggtc agctgattga acaccgttta aggaagagat tgtgaaaaag
    41941 tcgcttagtt tcgagtatta agaaaaattt tgtgaaaatg gatttctaca tttttgcata
    42001 aatttggctt aataccgaga aactggcttt tgaataccgt ttaaggaaga ggttgtaaaa
    42061 tcagcggttt gacctgagta ttagagaaac aaacggaaaa atagctgctc atattttgcc
    42121 gttttttgag ctaataccga aggtcagctg attgaacacc gtttaccaag aggttgtgag
    42181 cctaccattt aactccgaat cacaagaaat aatttcaaaa acaacttctc ctgtttttgg
    42241 ttcggttggc ttattatcga gaagtagcta ctgggagtct atttatcaaa aaaaggaatg
    42301 cgacataggt cacacccctt ttttattttc tttgaaacat ctttcggtac tcgacagcat
    42361 acaagacaaa agattgtaaa agaccgtaag tgatgacggc aaaaatgact ctcacccaca
    42421 gcgttggtaa ttctgggaaa agcagcaaaa ggatcatgcc aacatagatg acagcagtcg
    42481 ctactttgcc ataccacttc gctccactta atttgatatt tttagatagc ccgattaatc
    42541 cagctacaaa cataaagcct tcttttatca agaatagacc aaacaacagc caaagccacg
    42601 gtcgatacca tgccatagac aaaatcagaa aaagttgtgt caatttatct gcaaacggat
    42661 caagcacctt ccctaagtct gttacctgat tatatctccg agcaatgacc ccgtctagat
    42721 aatcagacaa acctgagaca acaatgaccg ctcctgctat ataaaatgac tgtctcagca
    42781 caaaataaag ataaatcggc agcaaaaaaa tacggaatat cgataaaaga ttcggaatcg
    42841 tttgtatttc tttacgccag tctgtgctta atttcatttt atcgccctac ctcctcagca
    42901 ctcttttctt tttattttag cttacacggg gaataaaacc aatgatttca ctgtttttat
    42961 ttttctttac agacactgat attatgagct aatttgagat aatcacttgc tgttttcatc
    43021 gtttactctt ctttcttcct gtcgtttgcg catttctttt actagtactt tatgagcttt
    43081 ttttgatcct cgctctaggt cacgctgccg tttacgtgtt ttataatcga taaacagact
    43141 gtctaaatcg taacctgtcg ggtacagatc ttctgctgaa cgcaccagac gaacacgctg
    43201 acgaggcacg tggattattt ttttgtctaa atatacatca atcgtatctt cacctatatc
    43261 ttcataaact aacgcttctt tttggtattc attgacaaat atccgatcac cttttgcaaa
    43321 gagctgacgt tcttttttat tctccttcct atttgtaact gctgtcttag gaagaaattc
    43381 ttttttctgt gtccgatagg ctttttccgc caagtaacgc tctgcttgat ggatcaattt
    43441 catcgacata tccattttat gagcaatcca caaagcttgg ctttctcctg tttcttccaa
    43501 cccgcaactg atatttagga cgaagtgctt cccgatcgaa agccatagca gccggcacaa
    43561 aatcttcatg atctccggcg aatttcttga tttctccata atgggtcgtg gcaatgatca
    43621 atgccccttg ctcatacata gattccatga ttgcgatcgc tagtgcagcc ccttcatttg
    43681 gctctgttcc actgccaatc tcatctaaaa gtaccaacgt attccttttt atctttttca
    43741 aaatagcagc gatgttttgc atatgaccag aaaatgtact caacgcattt tctaaatttt
    43801 ggtggtcacc gacatcgacg aaaatctcat caaagacagc aagctctgtc ccttcttttg
    43861 ccggaacttg cataccgaac atcgccatca aagtcaataa accgactgtc ttcaacacga
    43921 ctgttttgcc tccggcattt gctccggtga taatcaagcc tcgatagtct ttaccgattt
    43981 caacatccaa tggtaccgca tgctcaggta aaaatggatg tctgccttgc ttgataatga
    44041 tatgttccga tttattgatt ctaggtgtgc ttccattgat ttctcggcta aattttgccc
    44101 gagcaaagat gatatctaac accgtgatcg tttcgatcaa taaatcgatc acttcttcgt
    44161 tttctgcgat agcaccagtc aatgccgcca ggacttgata ttcttcactg atttcttcag
    44221 cttttaacaa ctgatagtgt tcattcaatt tagaaatcac agttggttca ataaaaaccg
    44281 tcgttctttt atttgattca tcgataatcg ttccatctat tttattttta tagctggctt
    44341 tgataggaat cgtatagtat tcgccttttt gaacaatcat tgcttcttgg atcatttctt
    44401 tgtttttcgg atgacgcaag aatttcagta attttgactg gatttctttt tcgattagtt
    44461 gtaattgttt gcgtacttta cgcaaattac gtgacgcgtc gtcactgact ttattatttt
    44521 ttattttctg gtcaatcaac tcttctacat ttatcaaatc aggaagatgt ttgctataag
    44581 caaacaacaa gggtgtctgg tattgattct tatcaaaaaa ctttgtgatc atccgactgc
    44641 ttctaagaaa atccgcatat tcgatcaaat cggttggctg cagaatcaac ccttttttga
    44701 cttgttctgt cagtgcatcg atcctcggca accccataaa tggaacatgc tgactgcttt
    44761 ctaagatcaa gcgtgcttct cttgtttctt cttgacgatc caagacagtt tgtaggtttg
    44821 tcgagaccgt catatcgctg attcgttttt tgctatagtt tccaattgct cttgcttgga
    44881 tttccttttt gatccgatca aattgtaatt gactaatcat ttcgttgtcc atattctctc
    44941 cttttcttga agagatacct gtcccaaaat aaataagggc ctagagataa ataaaaggat
    45001 agaacacagc ttaaaaaagt catgtcctat ccttattaga atacaaagat attgttttcc
    45061 tgacaggtac cgtttcataa gcacaccaaa taaactttgt ttattggcta ttcgacttac
    45121 tgattagata ccagctacac tcacaaaaca taccccattc ttctaggtct tatttatttt
    45181 ccacttaact atagcaagac acattttggt tgtcaaactt tagtatgttt tatagattta
    45241 caccattaat gaatagacac ctaccgcgat aattgctccg atcattggac ctgcgattgg
    45301 tacccatgaa tatgcccaat ctgaagttcc tttattagct attggcaata ccgcatgtgc
    45361 taatcgagga ccaaagtccc gagcagggtt gattgcatat cctgttggcc ctcctaatga
    45421 aagaccgatg gctaagatca atccacctac agcgaatacg ttcgttccag gtgcaaattc
    45481 gttttgtcca aaagctagca acccaagaac taatacaaat gtaccaatta attctgtgat
    45541 cacatttgct ggataattcc gtatagcagg acctgttgca aacgttccta aaatcgcgct
    45601 ttcatctttc gttgcattcc aatgtggcaa ataagaaagc caaacaagaa tggcccctgc
    45661 aaaagctcct aaaacttgcg caatgatgaa aggaacgaca agattccaac taattgcccc
    45721 tgtcatcgcc atagctagag agactgcagg attcaaatga gctgggccca tgaatccaga
    45781 aatatagaca gccattgtta cagcgagtcc ccaaccaaat gcgatgacaa tccagccaga
    45841 agcctgtgct ttacttttgt ttaaattcac agccgcacac acaccatctc caagcaaaac
    45901 taggatcagt gtcccaataa actcccccat gatttgagtc atatcactgt tcatttattt
    45961 ctcccccttt agttcccgca gatctgattc tgcaatcaat gcttctaaac gtttagtctg
    46021 ttgcgccttc tcctcttctg tccattcaaa atatccagca attgcatcga tcacaggttg
    46081 cttgatctca tcaagctgat ctcgttcaaa caacagatga tttgtacgac gaatgagata
    46141 atcgcctggg gccaaaacca tttcttcttc taaaccataa cgtaaccgag ctgactcagc
    46201 gagactcaaa ccaggataag gtgccatttc ttttgctaat tcaaagatac gtcgggcatt
    46261 agtcccgtaa aaatcagcga tataagtagc atcttcttcc tctaaaccgg ctgctactcc
    46321 ttcttttgct aattctgtca ctgtttcttc taatttagtt ggatcgaagt ttccacctga
    46381 aatctgatat tttttagagt cgatttcttt cgtctctatt ccgtattctt cttttaataa
    46441 ttggcgtatc aatcgcaaag ctccttctgc catcttacgg taatcggtga ttttcccacc
    46501 agataaagtc agcaaaccat ctggttctct ttctaaagaa ctacctctgg aaatcgtaga
    46561 aggtgctttt tcatcacggc tgttttccaa atggttcaac acgtcttcta cttcagcacg
    46621 agaaactttg ttttccttat attcacttac tgtatcgaca actttattga agctcttgtc
    46681 tgaaatcgat ccattatctc caccattgta atcagaacca gaattaccaa tcaacagtgg
    46741 acgaagtcct gcccagcttg cttcgatatc attcaatgtg atatttgctt ctggatagcg
    46801 atgattgatc acatccaata gataatctac atcttcttgt gtgactttcg gatccgtaaa
    46861 atctccttgg taatccgtat ctgtcgtacc aaagtaagtc ttgttttctc ttggaatagc
    46921 aaaaaccatc cgcccatctt gctttcctgt atcgaagtat gtgggttgcg gtacaggcag
    46981 ttttttcgca tctacaacta aatggatccc tttagttgga cgcattttag gagagactgg
    47041 acgcgtaaaa ttcaagtttc gtactttatc tacccaagga ccactcgtat tgatcactaa
    47101 ttttgctttg atttcgatca cttcatctgt cagcaggtca cgagctttga cgccaacgat
    47161 ttgatcgcca tcatataaaa agccaaccgc tttcatttta cttactagat aagccccatc
    47221 ttcggcagcc tttttgatat tatcaatcac taaacgggca tcattgttgc ggaaatctag
    47281 atagacaccc gcgcctttta gcccttcttt tttcaaaaac ggttctcttt ccaatacttc
    47341 ttctggtgtc agcgtataat tctcatattt ggttcctgtc acatttgcca gtttgtcata
    47401 aaggtccatt gctactttga ccgagaacat attgaaggtt gttgctcctt cgccctcata
    47461 gattggcaaa agcattggat ctggttttgg gatgtgtggg gcaatacttt gtacgactgc
    47521 acgttcacca actgtgtcag ctactacttc cacatcaaat gtcttcagat aacgaatacc
    47581 gccatgaaca agtttggtcg agcgagagga agtcccttct gcaaaatcct gcatttcgat
    47641 caatcctgtt ttgattcctg atgctgctga ctggactgcc acaccggcac cagtgattcc
    47701 gccaccgatg atcaacagat ccaattcttc ttgctgcatt ttttgaatgc tatcttgtct
    47761 tgtcttgttt gaaaacatat ggatttcctc cttattcaaa tgcttgggta gcttttactg
    47821 cttttttcca accgttgtaa agtttttctc ggcgctcttc ctccatttga acttcaaata
    47881 gcttgccagc ttcataaaat tcgcggatct cgtctgtatc tttccaataa ccgacagcta
    47941 atcctgctaa aaatgctgcc cctagcgccg ttgtttccaa gttttctgct cgttggatcg
    48001 gtacattcaa gatatctgtt tggaattgca tcagatattc attgtttgct gcaccgccat
    48061 ctactttcaa tacaggaatc ttgattccag tgtcatcttg catcgtatcg atgatatctc
    48121 tcacttgata agcaatcgac tgtaaagtag ctttaatgat atcttctttt gtcgtaccac
    48181 gtgtcaagcc aaacatagcc cctcgtgctg cctgatccca ataaggagca cctagcccaa
    48241 cgaatgcagg aacaacataa acctcatctt cgcttgtaga gcgtttcgct gcgtcttctg
    48301 aatcgctagc tttttgcaac atttgaagtc catctctcag ccattgaatc gaagaaccag
    48361 ccacaaaaat acttccttct aaagcatagt aaacctttcc atttatgccg taaccaattg
    48421 tcgtcaataa attatttttg gataattgcg gtttttcacc ggtattcatg acaataaatg
    48481 aacctgtacc ataagtattc ttgaccatgc ctggttcgaa tgccatttgt ccgaataatg
    48541 cggcttgctg gtctcctgcc atccctgaaa ttggtgcttc gcttccataa aaatgataac
    48601 cttgcgtata accataaact tctgagttag aagtgacttt tggtaacatc actcgaggaa
    48661 tatttagtaa atctaaaatc tcctgatccc aatctagctg atggatattg aacaacatcg
    48721 tccgactggc attcgagtaa tcagtaacat gtgcttgtcc atcggtcagt ttccatacta
    48781 accaagaatc gatcgttcca aacatcaatt cgcctttttc agctcgttct tgcgcgcctt
    48841 ctacatgatc aagaatccag cgtatcttcg ttgctgagaa ataggcatcg atcactaacc
    48901 ctgttttttc gtggaaaaat tcttgatagc cttcattttt taagttatct gcaattccag
    48961 ctgactgtct ggattgccaa acaactgcat gatagattgg acgtcctgta tctttttccc
    49021 atattactgt tgtttcacgc tggttcgtta tgccaatccc agcaatctga tttggtttga
    49081 tccctgattc gatgaacgct ccggcaataa ctgactgtac agaattccag atttcatttg
    49141 catcatgttc cacccaactt tcttgcggga aatattgcgt gaactctttt tgagaactgc
    49201 cgatgtgtct tcctttcttg tcaaagataa ttgctcgtga gctagttgta ccttggtcaa
    49261 tcgccatgat atacgttgat tctgtcattg tgattccctc cattatatgc aaacgctttc
    49321 tttaactact cttatcatac tactgtttta aaaaaagaaa ctgtcaaaat tttattcttt
    49381 ttttcttttt ttgacagttt tttattatga agctattttt taatcaattc ttttcttttt
    49441 tcagcgtctc tattcttctt ttcaatcgaa taatgtcgta aggtgacgca aattctgaca
    49501 gataatattc acactgtttt ttgtttctct ggcaaaatgt gatcaccaaa tcatattctt
    49561 taccttcttc ataacttcca atctcaatac cgccgattcc cctcagttct ctgatcagat
    49621 attgttgaaa agcaatacga taaatcggca gactttctaa atcgattcca atcactactg
    49681 gttttgcaat ataaaaatcg atcatcaaaa gaacattggc atactggatc atcaggtaac
    49741 taagctcact atcttcagct cttttcctcg gcaattgctg caccgctgtt tcctgtaatt
    49801 tcgccagtaa ctgtgttaag gaatgcccga gcattttctg ctgctgttcc aaaagccgat
    49861 cacgctcata gacttcgatc tttccaacaa agaaaaacca ttcattgttg atctgggaaa
    49921 tttggtagcc tactgccttt tcttctttga ttttcaaacg acgaggacga taatgccata
    49981 acatcgtttc ccgaatgtaa gtatctaaaa cagcggttgg taactttttg ctccgtgtca
    50041 aatcatactg ataaaatatt tcttcgtcta ctatcgaaaa agaaacaaaa aagctgtaaa
    50101 acatcattgt ttcgtataca ttcagttctg ctgctgttcg actaagatgc atcacgatga
    50161 ttggatcgat tgcttgataa agccggtccg actgatagag taatttcttt tctttcaaag
    50221 ttgcgtacgt acttttttca ttcaatagtc tttttttcgt aatcccgagc cagcaagcaa
    50281 tctgtgcttc tgctgatgcc gtaaacgttg tttctaatac acggtccaat cctagaatga
    50341 acggtctttt ttctggtgta ttttgcaaaa aaagcggtcg ctgatcttcc gggatatatt
    50401 ggaatagttc ataataaaaa tatcgtatct gtagctcttc tccgtataat tgaccgtttt
    50461 tgatttgtaa tccaaattcc tctaagagct ggttcaattc tttgatcttt cgaaaaacag
    50521 aagactcact gatattaaac gctgtcgcta atcgaacgat ggatacttgc tgatgttcta
    50581 ataaatagac gagcatctta aacttcaagc tttcctgcaa taaaaacaat agaatttttt
    50641 ccaaacttac atcttcgtcc aacacaagca gcgcctgata ctcatttcgc tgtacttcta
    50701 cttttttttc catcattcgg ccaatcaatt caatatcttc caaatattga tcaaacgagg
    50761 cttttgacag ttggacatgc tctctcaaac cattgataga ggctgttccc ccttgcagaa
    50821 caaggtttcg caaaatcagg atctgtaact gctcttttct ttctaataat gcttcaatct
    50881 tcataggcag tattcgccac ctttgaccac accatatctg gcacacggta ttctggctgg
    50941 aaaaatagct catgttcttc taactgttca aacgtccatc cttcttctac tgcaattgtc
    51001 agttggtcaa gaaggtggaa catctctttt ttgctttgga gctgtgctcc aagaatcttt
    51061 ttttcagaat caaatagaaa tttgagttta actttgcttt gatttattgc tgtaagcttg
    51121 tagtctacgt atcctgtttc aattttttgg ggataaaaaa tgctctcttc ttctgtcaaa
    51181 cctacgcttc ctaagaaata gccaaaccac tgattgccga ctggacgata ggttccctga
    51241 tcctttgtct gtgagccact aattgtcttg ctgacatttc gtgctgtacg gacagcgtta
    51301 tttactagag aaacatacat tttttcattt gttggacgaa aagttacttg gatcgcatcg
    51361 ccaatcgcat aaacgtcttt ttgagacgtt tgcaagtagt catctaccca gatcgttcca
    51421 tcatcattca atgttaattg ttcttcccac atctgattgt cgggacgaga atgattagcc
    51481 agcaaaacaa gatcatagtt ctcctttctt ttttccgtct ctacaatagc ggtttcattt
    51541 tcggtcaaag acacaacttg ttcttctaaa aaaagtgaaa gcccttgatt tttcatttcc
    51601 tttactaacg gttctgtcat ttcaggatct agataacgaa aaagaattga ctttcggctt
    51661 tcgtaaagat ggatttgttt ttttcttgat gccaaccctt cagctatttc gattcctgct
    51721 tgtcctgcac caataattgc aattttcttt gcattcatga tcttttgctg aagagccgtt
    51781 acgttttgta tctttttaac tgggtgaata agcgttgatt ctgcagaaat atttcgaaac
    51841 ttttgatttg aaccagtcgc tagaatcaaa cgatcaaaat ccaatttttt tcctcctgct
    51901 aaaacgactt ttttttcttt caatcctatt actgtctttt ctgtgtaaac atggattcga
    51961 tagcggtcag tcagttcttc tttcgtgatc caagaaagtt gtttttcact ggtaaccttt
    52021 ccttttaata tcaaattgaa cccgctggga atccatccta acttcgattg cttttcaatc
    52081 aaatatattt cacttgttgg catcaattgg cgaagataaa tagccgcatg aatccctgca
    52141 aacgacccgc cgacaattac aatgttcatc tgcattcctc tttcttataa ggtgttctac
    52201 tgctaacttc tcaatcagaa aacacagtat tcataactcg ctttcaacct tatgatacat
    52261 agcgatgcag cagaatcaac cctctccatc ataccgtgtt ctaggtctgc ttctcaataa
    52321 tgtactagtg gacctaattg aagagtatca tttctgaaga gatgtttcaa aaaatgtact
    52381 cttataaaag aaaaaaactc gccttttacg tacctctgtc caaataggaa acagaaaaga
    52441 acgaaaagac gagttttagc gtgtgtaatg taatagttag aatagacaca tcatttattt
    52501 ttaattcttt aatgcttcaa caactgcttc gcctaattga tgagcagatt gaggattttg
    52561 ccctgtaaga agccgcccat caatttgaac cacttctgaa aaagctgctc cagactgata
    52621 gtttgctcct ttttctttca aagcatcttc tgctaagaat ggtacttcat tcgttgtacc
    52681 atttgcttcc tcttcttcat tactgaaccc agtcactgta cgtccagcta aaatagattt
    52741 tccagactca tctgtaactg gcaacaatcc tacaacaccg tgacaaacag ctgagaccag
    52801 cccttcattt cgatagattt gtgaagcgac tttcccaaga tctattgctt ctggaaaatc
    52861 ccacattgtt ccgtggccac cagcataata aaccgctgca taatcatttg catccacatc
    52921 atcaggacgt aaggtatttc ccaaggcccg atttctgaaa tcttcatctt cataataggc
    52981 ccaatcgatt tcatccatat tcaaaacact ttgcggttct aaaggtacat atccgccttt
    53041 aggactgaca aaatctgcat cgatcccatt ttttactaat acatcataaa aatgagtcag
    53101 ttcacttaac cacaaacctg taggacgctc gatcttttcg tacttttcta catttgttaa
    53161 tacgatcaat actttcattt tcccatcctc tttctataaa aattgcgtta ttgataacgt
    53221 gctttcaaga aattatataa tgggcttggc aaataaagct ctttatatgc tttttcaaaa
    53281 ggtttcgtta caaattcatc ctcacggttt tccaataatt tctctgccca gtcaggatcg
    53341 acgatcattt gctgtccaat tgctgcaagt tctgcatatt tcaatacctg ctctacatct
    53401 tcgcgattgc ggatcccacc gacaccaact aaagggatct ttccttgaat cgtgttatgg
    53461 accacttcaa gaatcgattt atcgttgtat ttatcagaac gagctattcg atcatacata
    53521 tttaatgaaa catgcagata atctagtttt gactcacgta atcgttcaag aagccagatc
    53581 gtgtcttcaa aacggatacc tggtgtttca tattcttctg gtgagaaacg ataccctacg
    53641 atgaatggtt ttttagcata ttgttcaacc gctgagaaaa ccgttgttaa taattcttca
    53701 ataaaacgat aacgattttc caagctaccg ccccattgat cctctcgtct attcgaatgc
    53761 ggagagaaaa attgttggat caaatacgta tttgctccat gcaactcgat tccatcaaat
    53821 cctgattcaa tcgctctacg tgtggctttg ccaaatgctt ggattgtttc aacgatttct
    53881 tcttcactca ttgctcgtgg tgtttccgca tcaggacgtt ttgctgcaac tgcacttgca
    53941 gatacaggct gttctccaga taaagttgct cgacttgtca ttcgtccacc gtggaaaatc
    54001 tgaataatcg cttttgcgcc ttctccttgg attgctttcg ctagctcact taatcgagga
    54061 atcatttcat catgagcgat gctgagttca cctggccagc cttttccgat atcttggaca
    54121 tttgcagcac cagtaataac agcaccaaga cctttagaac gttgtttata ataagtgatc
    54181 tcatcttcgg tgatcactcc attgaaaaag ctgagttgtg tcgtcatagg cgacagcatc
    54241 aatcggttct tcaattgaac tcctgaaggt aaagtaattg attgatcaaa ttgtgacatt
    54301 gtcattcctc ccataattcg ctctgattaa cctatcgaga atcatctatc attaaaccac
    54361 tcgattagtt gtacatgcaa ctaatttgct taaaaaattt tatttttttt gtaacttatc
    54421 tgttgcatca ttcattgtct gggctaattc tttacttgct tcatttccca aaactgcttt
    54481 gtatgactga tgaagttctt cccagctacc atcgacttcc ttagcaaatt ggcgtccttg
    54541 ttctgtaaga taaactaatg acaagcgacc ttcgctaact gttttgacgt atcctttata
    54601 ttctaatttt tcaacaaaac gagcaatcgt tgatggggcg gaatagagca tttgtgcaat
    54661 atctttttgt ggtattccat ctttttcttt taatatcaat aaaataaaac cataagttgg
    54721 tgccagacct gtccttctaa acgcttcatc agctaattta ttcaacatgc gatctagctt
    54781 tttcacgcta aagaaaagac attcatctaa cgctttttca attggctttt ccatttcatc
    54841 ccctccattg gcgtacatat catagtatga tttttatttt ttgtcaaaaa acacggaata
    54901 atgtccgcct cctctgtcta gatttgaaaa aaagaagtag ccatcttcaa agaatttcgg
    54961 cttgttttcg acaaaactcc ttctggaaca ccgtgaatca gaggttgtga aaaagtcgtt
    55021 tagttttgag tatgaagaaa aattttgtga aaatggcttt ccacattttt gcataaattt
    55081 ggcttaatac cgaaaaactg gcttttgaac accgtttata tcagaggttg tgaaaaagcc
    55141 gtcctgcatc aagtaataag aacagccaaa caaaaatagt tcctcatatt ttgtcgtttt
    55201 ttgagctaat gccgatggtc agctgattgt acaccgttta ttgagaggtc gtgagcctgc
    55261 cgttgaactc cgagtcacaa ggagcaattt caaaaaacag cttctcatgt tttatgacta
    55321 tggtgacttg tgatcgagga gttggcagga gaatactgtt tattcgtaaa gaaggggctg
    55381 tgacaagact tttgtcacag tcccttattt ccttttatta taggatttcg ccatttgatt
    55441 ggatgacttg tctgtaccaa tcaaatgatt ttttctttaa ccgtttcttt gtgccattcc
    55501 cttcgtcatc ttgatcaaca tagatatatc cataacgttt tgacatttgt gaagtagaag
    55561 cgctgacgat atcaatgcag ccccacgacg tataaccaaa taaatctact ccatctttga
    55621 tagcttcctt catctgctca atatgctgct tcaaatagtc aatgcggtaa tcgtcttgaa
    55681 cggttccatc ttcttctact ttgtctatag ctccgagtcc gttttcagct atgaacagtg
    55741 gtttttgata cctatcatat aattgattca aagcaattct caaaccctta ggatcaatct
    55801 gccagcccca ttcacttgat ggaagatacg gatttttaat tcctccagtt attaagtttc
    55861 cttggacccc ttcccaattt tctggatgag cactgactgc actagacatg tagtagctaa
    55921 aagaaacaaa atcaacaggg taggctgcta agatttcttc atcacctact tctattttta
    55981 agttgacacc tttttcttca aacattcgtt gcgtatacgc tggataatat cctcttgctt
    56041 gaacatcact aaagaacaaa gaagttcttt gctgctcaat tgttttttga atatcatccg
    56101 gatcacaaga atacggaaca agaagctgat agctgatcat acaaccgact tgtgaacctt
    56161 tgataatttc atgacaagct tttgtagcta aagcacttgc tataaactga tggtgtgcac
    56221 cttggtattt cgcttgttcc aaatggggat ggttttcttc aattactcct acgctaacaa
    56281 aagggtgatg ttttacacag ttgatctcat tgaaagtcat ccaatatttt actttatttt
    56341 tatagcgttt gaatactgtt tcagcgaaac gaacgtaaaa ctgaataagc tttcgattag
    56401 gccatccacc atattctgta accaaatata acggcatttc ataatgggaa agagaaacga
    56461 ctggctcaat cccatatttt agacattcgt caaacacttg atcataaaaa aataatcccg
    56521 cttcattggg tgtttcttca tcaccatgag gaaaaattct tgtccatgcg atagacatcc
    56581 gatagcattt aaaacccatt tcagcaaata aacggatgtc ttctttaaag tgatgataga
    56641 aatcatttcc gtgtctttta ggataataat acttgctgtt gggatcctgc gccgctttta
    56701 cttgctgatg cgtcatcata tgccattgat ctgtccattt ttctttaggt aaatcgctgt
    56761 cgaatgtata cacatcagac acagataatc ctttttcatc tacatcccat gcaccttcac
    56821 attgattggc cgctgtcgct ccgccccata aaaatccttc tggaaatttt ttcatatcga
    56881 tccccctaaa cttgctagtt ttcttgtttg attatttttc ttatgactag taatgatatt
    56941 tttgaatcta ctactatttg taacaataac cattgtggtc ggatcatatc cctctttgat
    57001 taattcttgc aaatctactt ccatcaataa atcgccttta cgtacttttt ttccatcaga
    57061 tactgcactt tgaaagtatt tgccatttaa attcactgta tcaataccga tatggatcag
    57121 tatctctgct ccttcttcac tgactacccc aattgcatgt ttcgttggaa aaactgttga
    57181 aacttccccg tttacagaac tgtagatttt tccgtccgtt ggaatgatcg caaacccttc
    57241 tcctaaagat ccactagcaa atgttggatc gtttacttca gataaatcaa ttatttcacc
    57301 tgctgcagga gtacttaaca ctatatcctt tgaggattct tcttcgattt catccttgta
    57361 gccgaaaaga aaagttagca tacaggaact gatcatcgct aagaaataac tagcaactag
    57421 ccaaacaaat ccttcaccaa tataaatcgg gaaagacaaa atacttcttg tagcaactgc
    57481 caaagcaccg gctcctgcgg caccagctac tccaccaaat atagctccac atatacaagc
    57541 aatgtaaaat ggtcgtttat atttaagagt cacaccataa acagcaggtt cagtgattcc
    57601 aaataatgct gtgataaatg cagataaaga taaacttttt actttcttat ttttcgtttt
    57661 caaaaatacc ccaagtactg ctcctgcttg cgcagctacg gcagtcatac atgctggacc
    57721 tagcgtatca cgaccgtaga aagaaagatt gttggtcatt actggtacaa gtccccaatg
    57781 taacccaaaa atcactaaca cttgccaaaa tccgccgata acagcaccag ataaaacagg
    57841 gctaaagtta taaacagcta aatatccagc tgcaatcagc tgacttgcat aatctgcaat
    57901 tggacctaca actaataaaa taattgggac catcacgatg atacaaatga atggtgtcaa
    57961 taaatttcga atactttcgt gcaagctttt gcgaatctta ggctcaatat aagaaagaac
    58021 ccatacagct aaaattgctg gaataaccgt acttgtatac cttgctagaa tcacgggcat
    58081 ccctagaaat cgcaaggtta tgctatcact atatgcactg acaatcgtag gataaacgag
    58141 agctgctgca ataaccatag cgatgaaacg atctgtattg aacttttttg cagcagtata
    58201 tgccaaaaat ataggcaaga aatagaaaaa tgcatcagct gctgcattca aaatgatata
    58261 agttcctgat tcagtagtta ataaattcaa actcgtacat aaggcgagta ctcctttgat
    58321 caaaccactt cctgccaaga cgcctaaagt cggggcaaaa acagcggcga tcatgtctat
    58381 aaaagagttt aaaatgcctt ttttctcagt tcctctcttt ccactagatg catcatcttc
    58441 tagtttcatt tgtttggata atacgtcata cacttcattt actgcatttc ctactacgac
    58501 ttgaaacatt cctccagctt cgactacagt gatcacacct tctagttgct ctaccttctc
    58561 cttatctgcc ttcttattat cttttagggt aaagcgtaac cttgttgcac aatggtaaac
    58621 attgctgaca ttttcttcgc ctccgacaaa tcggacaata tctttcgcta attgtgaata
    58681 gtccattttt acttccccct tgttaattaa tgccgtagca atccttctta caagataagt
    58741 atagttctta gaaaacggtt tctccattca attagtaccc cttctaatag cgaacgaaac
    58801 tgtacaatag tgtgtatggt ttacttagcc gagcaaatca ctcactgctt gaaagaaaac
    58861 aagtatatcg tttctcttat agtcaaagga gacaaaaaaa acacgcttga tcttcctttg
    58921 ctgatgtata agtggtatct caagctgata ctctgtgatt tttgtttcta cttggctttt
    58981 gataagacag cacaacaaag tctcggcatt taacggaagc ataactaggt cagaactagg
    59041 agagattagt tgctgaatgg aatctatctt tttatggatt tttatttgat tttcttcttt
    59101 gtagtaatga tcaagcagtt ttgttttctt tttttcttca actatggtca gcttttcacg
    59161 caaaatctga taaatctccc aagattcaaa ctcatctaga acactaggta gataaatcaa
    59221 atcatcccac tcaaaaataa gctcttgctc agtagtcaaa attgcatcat attccgttat
    59281 tttgtttgat tgatttttga aaagatacac tggttctatc cggcattctt tgatccacat
    59341 gttcatactt tgttgaatct gtttttttag tgtattcaaa atactaattg gctggttact
    59401 aacaatcagt atctcccctt gttttcgaaa aggattcaac gcttcggcaa gatacaaaac
    59461 aaaataagcg ctttcagcta aattgggacg taattgaaaa tgtattggga agaaacgccg
    59521 aattaaataa atttctaaag gataacggga gatgatcttt tgtacaaaat gattcatggt
    59581 attatgtccg gcatccatcc tcgttttcat ttgtttgata tgggttagca aaaattctgt
    59641 ttcatggaac aataacgaaa caggaagatc taaaaaacga ataatcgttt gctcaaatgc
    59701 tcttagattc ttctctattt ctttgttttt ttcatcatta atcatcaaat aatttaactc
    59761 taatagtcga ttttcaattg cttgtttttc ttgattggaa aaagaatagg gtagttctga
    59821 tgataacttt tttgtgaggg tctctaccat cggaataaat aatgtatacc tgtttgtttt
    59881 ttctaaaagt tcttctcttc gaagaatggt caaaacgaga taccgagaaa atcgtatgaa
    59941 gtcctctcct gaaacaatat aattatatga tataagaacc tcttttaatg tcttttggac
    60001 agtcggcatg atattggaaa aaattgtact gatttgttga ggaaaccttg cgtgttggat
    60061 caccgtttct gttccaacaa gagataagaa aattcttttt gaattttctt cagcaatgat
    60121 ttttagtcct tttgtcgacg aaacttccag ttctaattta ggattgcgct cgatccaacg
    60181 caaaatcgtt tttatttgta atgatacggc tgtttttgaa agaaagaaaa tgtctgctat
    60241 cttttgcata ctgacatacc ctttttgaaa caataagtac gtcacaatag ttgaagctcg
    60301 gaaatccatg ctggaataga ttttatgttt cgtcacctct tgtatgattt gctgtttagt
    60361 ttcagattca attgattcta aaaaataacc tgaacgttta tttgactgga taatgatttt
    60421 cttctcgtag atagcaagca tatttttgat ttcagtttgg atcgttttct tgttggtacc
    60481 taaagcttca gctaattctt cgcttgttat ccaatgattc ttatttctta atactacaag
    60541 aatttggagt tgtcgtttat ttaagtccat ttgcttacac ctcctctttg ttataaacat
    60601 acaggaaaaa cctctcaaat gacaatagtc acttgcagag gtttaaaaat catttgaaga
    60661 aagatctata ttcaaggttt ttaccattag actggataat ttgtttataa tattcaaaac
    60721 tatctttttt caatctccta aagcttccgc tcccatcatt ttgtctatcc acataaataa
    60781 agccatatcg cttcctcatt tcccctgttc ctgccgatac gatatcgatt ggtccccacc
    60841 ataagtaacc aatgatttgg caaccatctt ttattgcctc agcaatttcc tgaagatgta
    60901 tttttagata ctcacaacgt tttggatcgt ggattcgatt caattcatct ggtgcttcat
    60961 ctagtccaat gccattttca actatgaata atggtaattc ataacggtct gtcaattcat
    61021 tcatgacata acgaaaccct agaggatcaa ttggccaact ccaaggttcg ggagattttt
    61081 ccttcaaaaa gggattatcg aaaccatttg ctccgccagt atctacgcgc atttctgctt
    61141 cttttttata gacagcgcta cgataataac taaatgcaat ataatctgcc gtgttagctt
    61201 gcaagcactg ttcgtctcca tctaacatta ctggtgcaca gtcttcttcc ttccatgtcc
    61261 tgtaaatata agctggataa tgccctcggc acatgacatc ggagaaaaac catgaattgc
    61321 gtttaaattg cagtgaacca aatacattct ctggatcaca gtcatatgca taagtcgcta
    61381 atgcagaaaa gctacacata atacccatct tagcatttgg atcgattttc tttaataatt
    61441 gagtagcacg ggcgttcgct agaaacatat gatgacaggc ttgataaatc actttttttg
    61501 ttagtgtagc aatgggttca agtttatctc tagcagggaa tgtaaccaat ccagcagctg
    61561 cataaggcat acgaaaagca ttattgatct cattaaaggt catccaatat ttcactttcc
    61621 cttggtagcg ttcaaagacg gtctgcgcat aaaattcaaa aaagtcgatt aattttcgat
    61681 tttgccaccc cccatactct cctaccaatg ctaagggggt ttcataatga ctaagagtaa
    61741 ttactggctc gatatcatat cgatttaatg tattgattag tctgtcataa aatacgagac
    61801 cagcctcatt tggcttctgt tcgtctcctc ttggaaatat tcgtgcccat gagatgcttg
    61861 ttcgaaaagc ttttaaacct aactccttca gtaaacgaat atcctcttca aatcgatgat
    61921 aaaaatcaat cgcttcatga gaaagataat ggagtgattc gtctaaagca atctcccaaa
    61981 tctttttttc ttcattccac tgaatactag gatgctggtt catgatcccg ataagcgtat
    62041 ctgtcacatt aggtaatttg ccgtcttcca accatgcacc ttcacattga ttagcagcta
    62101 ctgctccgcc ccataaaaaa ttttctggaa atatttcctg atccataact atctctcctc
    62161 gctatcaaca atttcttact tgaagtatag ctcgattcat caaaacaaga tattctaact
    62221 atttcggcag aagagaagaa aaaaattgaa tgctactctt catttttaat atttatatat
    62281 aacaaataca taagacgatc cccgaaagaa aacactcgtt aaccatctaa gcaaacgatt
    62341 tattttactt ccaacacaaa tgcttcataa ggtcgtaaat caactttgcc gtctggtaac
    62401 tgtttcagag gataatttga cacaataacc cttaaagctt cacgtggaat agtgaacgtc
    62461 tgcttttcac tggacatatt acaaataacg agccattgct ggtcttcata tatgcgagaa
    62521 taggcaaaga catgctcagg cgtcttttcc actaattcat agtctcccca gacgatgatg
    62581 tcatgttttc tacgcaaatc gatcaatttc ttgtacatat aaaaaacaga atcttgatct
    62641 gctaacgctt cttctgcatt gatttcttta taatttggat tgactcgaag ccatggaatt
    62701 ccagtagtaa acccagcatg ttcacgggcg ttccattgca taggcgtcct agcattgtct
    62761 cttcctttcg ccttgattga ttccaagatt tcctcttttg taaatccaga agaaatcctt
    62821 tcgttataca tattgattgt ctcaatatct tttgcttccg cgatatcatc gattgggcaa
    62881 ttcgtcatgc ctaactcttc tccttgatag atataagggg tgcctttcat catatggagc
    62941 aagatagcaa acatcttggc actttctttt cgatacacat cgtcatttcc ccaacgagaa
    63001 acaattcgcg gaagatcgtg attattccaa aataaactat tccatccttc atcacctaaa
    63061 gatgtttgcc atttggataa tacttttttc agttcagaga tactcaaggg tttcaaatcc
    63121 catttgtctt tgccttcttg ctgatctaat cctatgtgtt cgaattggaa gaccatggat
    63181 aattcatttc ttttaggatt ggaataaagt ttagcgattt cggtagttgc tccccatgtt
    63241 tcgccaacag tcatcagatt tttgtcacca aaagttgctt gattcatttc ctgaagatac
    63301 tcatgcagtt taggtccatt acttgtgatt tctttatccg gttctttgcc aatcatatcg
    63361 atcacgtcca tacgaaagcc cccaatacct ttatcgatcc agaaattcat catttgataa
    63421 atctcttggc gaacctgttt atttttccaa ttcaaatcag gttgacgctt gctatacaag
    63481 tgcagaaaat attgccccgt tttttcatcc aactgccaag cagaaccact gaatattgag
    63541 cgcaatctat taggctcttt tccattgacc ggatcacgcc aaatataata gtcccgataa
    63601 ggattttctt tatttttacg ggcctcaatg aaccacggat gttcatcaga agtatgattc
    63661 accaccagat ccatgatgat acggatattt cttttctttg cttcttcaat cagttcttcc
    63721 atttcttcca tcgtaccgaa ttctgccatg attgcttgat aattactgat atcataccca
    63781 ttgtcatcat tcggagactc gtatactgga gaaagccaaa ttgcatcaat acctaaatac
    63841 gccagataat ccaacctgga aataatcccc cgcaaatctc caataccatc ttgatttgaa
    63901 tcttgaaaac ttcttggata gatttgataa acaactgaat ggtgccacca tttttgttcc
    63961 atgattttcc tcctataaat ggatgtttct attttttgct taatcattta ttttagtctc
    64021 tctcactaaa aattgtaacg ctttcaaata gctattgcac cttgttatcg gtaacagaat
    64081 aaaacaaaaa aacagctgag gaaattccct caactgtttt ttgaaaggat tgatttatgc
    64141 agggaagaat gctgatttca caacttcttt ataaacggtg ttcactcagc tgtcccttgg
    64201 tattagctca aaaaacggca aaatataaga aactattttt tcgttttttt ctctaatact
    64261 caggccagaa cgctgatttc acaacctctt tatttcacat tcaatatcca catgaatccc
    64321 acaaatacaa agaacagacc atacatgatt ggagatactt cttttcctct tttagctgct
    64381 aacatggtaa ttggatagaa gataaaacct aatgcaatcc catctgaaat actataagtc
    64441 aaaggcatgc cgatcaagat caagaatgaa gggatcgcaa tctctaagtt tccccattcg
    64501 atttgtcgca aagattgtgc catcaatact cctaccataa tcaatgctgg tgcagttact
    64561 tgtgaagtaa caactgatag taatggagaa aagaacaagc ctaacaagaa gaaaatccct
    64621 gtagtaatag ctgtgatacc tgaacgtcct ccaacggcga taccagctga tgattctaca
    64681 aatgcaccaa caggagaagt accaaataag gaacccgcaa gcatagctgt tgaatcagaa
    64741 gctaaagctt tccctacacg aggcatcttg ttgtctttca tgaaacctgc ttggtttgct
    64801 aatccaacta aagttcctgc tgtatcaaag aaagtcacaa gcaagaatgt cagcactacg
    64861 acccacattt gaagagaatt gatgtcccct acatgtttta atgcaactaa aaatgttggt
    64921 ttcaaacttg gtgctgcggc aacaatttca gaaggcattg gaatcaaacc tgtgaccaga
    64981 cctaaaatcg ttgtagctgc catcccgatg aagatgccac ctggtacacg ccgaacaagc
    65041 aagatagcag ttacgactaa tccaaaaatc gtcaaccaag tagaaccaac gttgagtgat
    65101 cctaatccta ctaatgtaga ttcattcgaa acgatgatcc caccttcgct cagaccaagg
    65161 aaagcgataa atagaccgat accgccagaa atcgcatatt tcaaatctgc tgggatcgca
    65221 tcaatgatca tttcccgcag cttgaagatt gtgatcaaaa taaaaatcaa agaagcaacg
    65281 aatacgccgg ctaatgctgt ttcccatgga atccccattc ctaaacaaac agaataagca
    65341 aaaaaagcat tgatcccaag agcaggtgcc gttccaattg gataacgggc taataccccc
    65401 attaaaacac agccgatcgc actggctaat gcagtcgcag taaacactgc tccttcatcc
    65461 attccagaag cacctaatac agttggattt acaaataaga tataagccat tgagataaat
    65521 gttgtaaatc ctgccagtac ctcacgtttt accgtggtgt tcaatttttc tatttcgaag
    65581 taggaaagta ttttttcctt catcttccat ccccctaaat ccgaacatta taaataaagt
    65641 ccgttaaaat ttcgatttat taataaaaca acgtgtttta tcatatacta aaacaaaaca
    65701 taagtaaaca aaaaattcgg attttaagca aaatccgaac gttccatctg attaaatccc
    65761 tttatctcaa agttcatgaa cattcacttt ggattacaag aatttcttta ttgtgttaat
    65821 aaagtttctt cattctagtc tcaaaaatca atagtgtgat ttgattttac catttcgttt
    65881 ctgttcttct ttgatgaatt cttttgtatc gacaggattc agattattta gtccgccaaa
    65941 acctgtatcg accatttttc cccagccttt tgtcttcttt tttcctgatt tgagattctt
    66001 tgctcgaagc ttctcactat gttttgctcg ccgatacttc aaataacctt ctaatcttcc
    66061 cattagatcc actgtttttc ctccttttta ttcaatcaga cgactcagct tttcctataa
    66121 caaaataatt gccttcaggg tcagaaaaat tgaaagtcaa gatgccattg ttatctgtga
    66181 tttctcctgg ttcttccatg ttctcgtaga tagtttcaaa ttcatccgta aaaaatacta
    66241 aagaaggcgg tggtcctaat acctctggtg aaaacttctg cacaaatact tttgggaata
    66301 cgcctaattc taattctttt gagatgctga gtacaatact cttaaattct tctggcaatt
    66361 cgattgtttc agaaatttct gcaccaattt tttctacaaa gaatcggcaa atcatctcta
    66421 cgtcattgac ataaagcatc acacgtgttt tttgtatcat catttgaccc ttctttctat
    66481 ttgattttct ttcctgtcta taaactgatt ccattatatc acgaaagcgc aaacaccttg
    66541 tgtttgatac tattcaagaa aaataaagca atagtttcta ggattgcttt cctccacaaa
    66601 ctttttttac aaaaaaaaaa cggcaatgaa tgccgctctt cttatttcga ttcttttata
    66661 attctgcttc tgattcatct ttggttagtg tgtgtctaac actagttacg ccaaatgttt
    66721 gatcgatttt ttccttcaac gcttcgattg cctgtgcgcc acgtgccgct tgttcagcat
    66781 tgatggattc aatgaccagc accgctgctt gtgtttcttc cgtcagcatc gttctttggg
    66841 cttcacgcca gttgagacag cgacagatcg ctccttgttc atcatagtag atgatttctt
    66901 ccggaagtgc cggagcatcg gaatctgcac ctaaaggcag gaatgattcg ccaccttggg
    66961 cttttcctaa atgcagattt cctgcgatct tttcgatatc ttctccgccg caaggtacgc
    67021 caaaagacaa ggaaatacta ttatagatat ctacaagcgg attgattggg aaaaattccc
    67081 gttcttgatt tgcacgtttc agtaaagctt caatagaaga acgtgcacct tttttagtct
    67141 tgaatgtggt aaatgcctga cgccactgag caatgacttc gttttggctg aatgtttctt
    67201 ctgtaaggta atttcttgct tcttttgctg cctcattcaa taattgatga tacgtttctt
    67261 cactttcttt tgttacgtgg ttatcgatac catttacgga taagatttcg atggttgctt
    67321 caggaaatac ttcccagaaa ttggtatcca ctattacttt tttcatctgt tcacacctct
    67381 tgattagaat aattgcttga tgaatagata gtctgtttgg ggatcatctc ccatataaaa
    67441 aacatgttcg ctgtatttga taaatccttt tttacgataa aaagcgagcg caacatcgtt
    67501 gtgttcccaa acaccgagcc aaatggctgt tttttgcttt tcttttgctt tttcaatagc
    67561 tgtatctagt aaaaatgtac cgataccttg atgtttaaat ttttttcgta catagattcg
    67621 ctcgatttct aaaaaatgat cagccatttc ctcagattga ctcgttcctg tatttagttt
    67681 caggtaaccc gcaggctctt gatcgatact cactaggaaa aaatcggttt ctttatcttt
    67741 tagctcttct gtaagttttt ctagtgcata agcttgtgtc aagtaatcag ctaaatgctc
    67801 cggtgaatta tcttttccaa atgtatctgt aaatgtttcg atactaattt gttgtagtaa
    67861 gtctacatct gaaggcgtca tttttcgaat ttcgtattta ttcacggctt cttcctttca
    67921 atagatcctt ttattgcctt ttttgacaaa attccaatct tcgctaacat tcttgcatac
    67981 tttatttaaa agatattcca attgttttgc ttcttcgtca gaaagaccat ttaatgcgac
    68041 tgcattggag tagttgtttt ctcgaatgat gaaaggatag atttctgctc cttttttagt
    68101 cggatagagt ttcttgattt ttttattttc tttatcttcc aaacgttcga tcatcccatt
    68161 ggattctaat ttcttgattg ctcgggcagt cgttgtacga tctactttga tcatttctgc
    68221 tagtttttct tggatgattc caggattttc gcaaatacgt actaaataaa gatactgtcc
    68281 tcttgttaaa tcgacttctt tgaattcgat attgctaata gaatctagcg ctcgggcaat
    68341 cattccgatg tctcgaagga tttcagccat ttcatcccac ctttattttg tttgtaagac
    68401 tagttcacct ctttttgttg caaatgcaac tattttcaat aaaaaaatca agttcttttt
    68461 taataactag taaagtatat tcttttcttc ttttacgaaa aagctcaatc acaatagatc
    68521 tggtttttgt tttctaaaat aagtaactgt ttcttcgttg ttcgcttttc tgatagcttt
    68581 ggcaactctt ctatactaaa aaatcccatg ttggaagttt cattattctc aataaacttg
    68641 gcctcttcac cgtggatcgc acaagcaaaa acaagtttat aatactgaaa aagctgtgga
    68701 atatcttttt gtttattcgt atcaaaaact gctcgcaact gagtagtttc tactgttaat
    68761 cctgtttctt catagacttc tttacgcaca ttttcttcag gagacaatcc tatttcagca
    68821 aatccaccag gaagtgacca ttctttcgtc cgtttatctt cgattagtag gaccttgcca
    68881 tcttttttga tataggcacg aacatctact tttggtgttg gatgtccttc tccaatcgtt
    68941 gtgagcgctt ttatttcttc gaaagtttct tttcctattt cactgatcag ttccaatgaa
    69001 atcgtccgga tctcctcaaa acgctcttta tcaaatacat cttttccata atatagacca
    69061 gcttgagaaa gtgcgagtag tcttttgtat tgagccaact tcttgttcat tctcttccct
    69121 tcttttttag caaatgtatg actatgtaat caagtataga agactggttt aattacggca
    69181 aataaatata atgaaatctg caataaataa aagaatgtta aaaaactcga gaacaaaaga
    69241 cattcgactt ctctggggct gtgaataaat ggcgttcaaa agcttgcatc tgcggtaata
    69301 agccccacca aacgaaaaat atgacgagct attcttgtct ggctgttctt attaattgag
    69361 gcaggacagc tttttcacaa cctccccact aaacggtgtt ctcctaccaa ctcctcggtc
    69421 acaagtcaca atattcatca aacatgagaa gctgtttttt tgaaattgct ccttgtgact
    69481 cggagctgaa cgacaggctc acgacctctc ggtaaacagc gttcaaaagc caatttttcg
    69541 atattaagcc atttctttgt tacttattgt cattgatttc tttttctaat ttatccaata
    69601 gtggagtgta atccacttga gtatagtctg ttacatcaaa ttcatatagt gttcctaact
    69661 ggaatttgtc atattttcgg tcggcgatga tctttttgaa tgcttctttc gtaatatgat
    69721 tggtcccttt cgttcgatct tccaatacgt caccatagtg ataatgatcc atgatatagc
    69781 tcaaatgacg atctgattca cgatcacgtt tgtaccgacg ttgccataac acttcaaagt
    69841 ctgctgttag gcgaatcgtg atcacctgat atccgtatct atctgctaat tcttggagtc
    69901 gtgctttttg cttatcacta aaaggatact cggacacaat cactttcttt cctgcttcca
    69961 tatacaaatc aagtgctttg taatagaaag gataaacttt cgtcctttct tgttccgccc
    70021 gttcttccaa attgttaaac ccgatggatt ctgcatagta ttccttgatt tcatcgagcg
    70081 tgatggcata catatctgga aatcgttccc gtagtttatt cataagatat gtttttcctg
    70141 taccaggact accagccaga agaatgaaat actttttcat ctcatttatt tcctaagtta
    70201 attggacttt ccaagttcat aaacagtcca atcttatgct tgacaatttc ttttactgct
    70261 tcatttgctg gaatcacatt atggcgaaga gacttgttga tttctgtttc actaaaggtt
    70321 tctttagcta ctttcgtcca gttgactaaa agctccgttc caacattgaa tttgcggatc
    70381 ccgttattga tcaattcagg ataatcttct tctttcacac ctgttcctcc atggataact
    70441 agggggacat caactactgc atcgatttct ttcagcaagg gaatgtttac ttctgttttt
    70501 gatttgaatt gtccatgatt cgtaccgata gcaatagcta atgcatccac gcctgtagct
    70561 tctacaaatt tcactgcgtc ttccggtctt gtatatacct tgtcattttc agcgacatgg
    70621 attccttctt ctgtaccgcc gattgttcca agttctcctt ctacggaaac accgtgcgca
    70681 tgggcatatt ctactactgc ttttgtcttc aggatatttt ctttgaaagg cagatgggac
    70741 ccatcataca tgacagaagt atatccgctg tcgattgctt ctttgatatc atcaaagtct
    70801 cgggcgtggt ctaagtgcaa gactgcatca accatttctt cttctgcaat agctgtcatt
    70861 acattgacta acacatgcat gccgatatat ttagcggtat cgacacttgt ttgaataatg
    70921 atcggcgaac ccatttcttt tgctgcgcct agcatagcag gaagcatttc taggttatgc
    70981 gtattaaaag ctccaactgt aaaattcaac tcttctgctg tttttgttac ttctttcaat
    71041 gttgtataca tcttttattg ccaccttatc tttttatttg tttcctttga tcgttacttg
    71101 ttttgcaatt tgtcgcatct catccatttt gcccatgtag taatcgatcc cttgatcatc
    71161 accagcacga gcagcatctg cacgcttctg gttatagagg ctcatcagtt ttttataccc
    71221 atctccgatc gtctgtttat tttccaaact cttttctaaa tattggatgg cttgatcgtt
    71281 cacatacaat tcagaaaatt taagtccaac ttgttcataa agtttcgcta atttatgctt
    71341 atcttgggta gtggaaatct cttgtttcaa ttgtgtaatc tctgattgaa tcttttcggt
    71401 cgcttctggc gatagggctt tttcttcttt tgccagaact tttttcggtt ctttttcttt
    71461 tttcttaaaa aatccaaaca tcattttccc tactttcaat taaaatgctt tcaaaactgg
    71521 gcttaagatc caggcatata agagtgctgc ggctactgta tctacatcgg ttgctgtagc
    71581 attgttgaat cccatcgaat taaagatcgt tactagcaaa gcgggcaata atgtaataaa
    71641 gaaaccatga gcaataccgc caatgatcgc tcctcggcgt ccgccaactg cgttgccaaa
    71701 tatccctgct gtccctccgg caaagaaatt ggttagcata ccaggtaaaa tcatcgctaa
    71761 gccgaacatg ggcaaagtaa acatggcaat aatcgtacca atcgttgtcg taatgaaacc
    71821 taaaatgaca gcatttgggc tataaggaaa gaaaactgga caatctaagg cagggatcgc
    71881 atcaggaact aatttcatag ctatccctcg aaatgccggc acgatttcac ctaacagtag
    71941 acgtacacca gctagcaata catacacacc cactacgaat tggatggctt gtaaaaaggc
    72001 aaacatgata tagttttgat ctcctgataa ttcagaagcg aatggttcgc ctgcaaataa
    72061 aacagtgatg atatatagcg ggaccatcac gaccatgaca gacaaataag tatcttgcag
    72121 aaattcgaaa tgtgtcggca acttgatatc ttcgatcgac tttttatttt cgcctttctc
    72181 accaaaaagc ttagcaacac cggcttcaaa taagtacccg atcgtacaaa aatgacctaa
    72241 agcaatatca tttgatccag tgattttacg aatgatcggt tgcgctactg ccggcatagc
    72301 aatggcgaaa caagctccga taaaaccacc gaccaagatc aatacgatcc ctcgcagacc
    72361 ggcaaaatag ccaaagactg tcgtcatcgt tgccatccat aaaattgctt gaccagttaa
    72421 aaaaatgtac ttccattttg tgaaacgagc caaaataata ttgaatacaa aaatggctaa
    72481 gaacgtcagt gcaatatctc tacctaaacc caaatcattc attgcttgac cattgataga
    72541 ttcaatagaa gggatgatcc cttgcatatg aaatccttca gtaaagattt tcccgaaata
    72601 agttaaactt ccaacgatga tgctcgaacc ggcactcaac acttgaaatc ctaggagtgt
    72661 ctttaacgtg ccagagatga cttgtcctgc tgatttcttc tgcagtagta gacccaacat
    72721 agcaatcaat gcaatggtga tcgatgcctg agtcaaaata ttttcgatga taaaattgat
    72781 aacgcccatg ttttgatgtg caccccaaaa gtta
//
LOCUS       DAZKKW010000003        67097 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.003, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000003 DAZKKW010000000
VERSION     DAZKKW010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 67097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 67097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..67097
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.003"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(114..854)
                     /locus_tag="ACG8PO_000131"
     CDS             complement(114..854)
                     /locus_tag="ACG8PO_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0205491.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
     gene            complement(984..1931)
                     /locus_tag="ACG8PO_000132"
     CDS             complement(984..1931)
                     /locus_tag="ACG8PO_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0205492.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            complement(2060..2824)
                     /locus_tag="ACG8PO_000133"
     CDS             complement(2060..2824)
                     /locus_tag="ACG8PO_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFQ0205493.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            complement(2828..3217)
                     /locus_tag="ACG8PO_000134"
     CDS             complement(2828..3217)
                     /locus_tag="ACG8PO_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0205494.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            3352..3894
                     /locus_tag="ACG8PO_000135"
     CDS             3352..3894
                     /locus_tag="ACG8PO_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HFQ0205495.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            4042..4675
                     /locus_tag="ACG8PO_000136"
                     /pseudo
     CDS             4042..4675
                     /locus_tag="ACG8PO_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(4809..4934)
                     /locus_tag="ACG8PO_000137"
     CDS             complement(4809..4934)
                     /locus_tag="ACG8PO_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HFQ0205496.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(5068..5961)
                     /locus_tag="ACG8PO_000138"
     CDS             complement(5068..5961)
                     /locus_tag="ACG8PO_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HFQ0205497.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            complement(6143..7069)
                     /locus_tag="ACG8PO_000139"
     CDS             complement(6143..7069)
                     /locus_tag="ACG8PO_000139"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HFQ0205498.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            complement(7154..7915)
                     /gene="pflA"
                     /locus_tag="ACG8PO_000140"
     CDS             complement(7154..7915)
                     /gene="pflA"
                     /locus_tag="ACG8PO_000140"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate-lyase-activating protein"
                     /protein_id="HFQ0205499.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            complement(8159..10390)
                     /gene="pflB"
                     /locus_tag="ACG8PO_000141"
     CDS             complement(8159..10390)
                     /gene="pflB"
                     /locus_tag="ACG8PO_000141"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFQ0205500.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            complement(10667..13117)
                     /gene="parC"
                     /locus_tag="ACG8PO_000142"
     CDS             complement(10667..13117)
                     /gene="parC"
                     /locus_tag="ACG8PO_000142"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFQ0205501.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            complement(13129..15174)
                     /gene="parE"
                     /locus_tag="ACG8PO_000143"
     CDS             complement(13129..15174)
                     /gene="parE"
                     /locus_tag="ACG8PO_000143"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFQ0205502.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            complement(15340..15774)
                     /locus_tag="ACG8PO_000144"
     CDS             complement(15340..15774)
                     /locus_tag="ACG8PO_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HFQ0205503.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            15901..16539
                     /gene="plsY"
                     /locus_tag="ACG8PO_000145"
     CDS             15901..16539
                     /gene="plsY"
                     /locus_tag="ACG8PO_000145"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFQ0205504.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            complement(16671..17546)
                     /locus_tag="ACG8PO_000146"
     CDS             complement(16671..17546)
                     /locus_tag="ACG8PO_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HFQ0205505.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(17628..18419)
                     /gene="codY"
                     /locus_tag="ACG8PO_000147"
     CDS             complement(17628..18419)
                     /gene="codY"
                     /locus_tag="ACG8PO_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HFQ0205506.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            complement(18437..19837)
                     /gene="hslU"
                     /locus_tag="ACG8PO_000148"
     CDS             complement(18437..19837)
                     /gene="hslU"
                     /locus_tag="ACG8PO_000148"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002409948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HFQ0205507.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            complement(19851..20399)
                     /gene="hslV"
                     /locus_tag="ACG8PO_000149"
     CDS             complement(19851..20399)
                     /gene="hslV"
                     /locus_tag="ACG8PO_000149"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HslVU peptidase proteolytic subunit"
                     /protein_id="HFQ0205508.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            complement(20436..21341)
                     /gene="xerC"
                     /locus_tag="ACG8PO_000150"
     CDS             complement(20436..21341)
                     /gene="xerC"
                     /locus_tag="ACG8PO_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HFQ0205509.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            complement(21539..22915)
                     /gene="trmFO"
                     /locus_tag="ACG8PO_000151"
     CDS             complement(21539..22915)
                     /gene="trmFO"
                     /locus_tag="ACG8PO_000151"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0047151 - tRNA
                     (uracil(54)-C5)-methyltransferase activity,
                     5,10-methylenetetrahydrofolate-dependent [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HFQ0205510.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            complement(22884..24962)
                     /gene="topA"
                     /locus_tag="ACG8PO_000152"
     CDS             complement(22884..24962)
                     /gene="topA"
                     /locus_tag="ACG8PO_000152"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFQ0205511.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            complement(25084..25941)
                     /gene="dprA"
                     /locus_tag="ACG8PO_000153"
     CDS             complement(25084..25941)
                     /gene="dprA"
                     /locus_tag="ACG8PO_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HFQ0205512.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            complement(25997..26764)
                     /locus_tag="ACG8PO_000154"
     CDS             complement(25997..26764)
                     /locus_tag="ACG8PO_000154"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFQ0205513.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDVRSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            complement(26757..27617)
                     /gene="ylqF"
                     /locus_tag="ACG8PO_000155"
     CDS             complement(26757..27617)
                     /gene="ylqF"
                     /locus_tag="ACG8PO_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HFQ0205514.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            27877..28275
                     /locus_tag="ACG8PO_000156"
     CDS             27877..28275
                     /locus_tag="ACG8PO_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFQ0205515.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            28512..29054
                     /locus_tag="ACG8PO_000157"
     CDS             28512..29054
                     /locus_tag="ACG8PO_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HFQ0205516.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(29137..29688)
                     /gene="lepB"
                     /locus_tag="ACG8PO_000158"
     CDS             complement(29137..29688)
                     /gene="lepB"
                     /locus_tag="ACG8PO_000158"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFQ0205517.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(29840..30067)
                     /locus_tag="ACG8PO_000159"
     CDS             complement(29840..30067)
                     /locus_tag="ACG8PO_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HFQ0205518.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            complement(30064..30582)
                     /gene="msrA"
                     /locus_tag="ACG8PO_000160"
     CDS             complement(30064..30582)
                     /gene="msrA"
                     /locus_tag="ACG8PO_000160"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFQ0205519.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            complement(30602..31315)
                     /locus_tag="ACG8PO_000161"
     CDS             complement(30602..31315)
                     /locus_tag="ACG8PO_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HFQ0205520.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            complement(31318..32214)
                     /locus_tag="ACG8PO_000162"
     CDS             complement(31318..32214)
                     /locus_tag="ACG8PO_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFQ0205521.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            complement(32372..33214)
                     /locus_tag="ACG8PO_000163"
     CDS             complement(32372..33214)
                     /locus_tag="ACG8PO_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFQ0205522.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            33346..33999
                     /locus_tag="ACG8PO_000164"
     CDS             33346..33999
                     /locus_tag="ACG8PO_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFQ0205523.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            complement(34062..34589)
                     /locus_tag="ACG8PO_000165"
     CDS             complement(34062..34589)
                     /locus_tag="ACG8PO_000165"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFQ0205524.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(34609..35556)
                     /locus_tag="ACG8PO_000166"
     CDS             complement(34609..35556)
                     /locus_tag="ACG8PO_000166"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFQ0205525.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            complement(35568..37451)
                     /locus_tag="ACG8PO_000167"
     CDS             complement(35568..37451)
                     /locus_tag="ACG8PO_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFQ0205526.1"
                     /translation="MKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKTS
                     LLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVRA
                     YEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIST
                     LSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDRY
                     FLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMRA
                     GVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRINDQ
                     TLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQE
                     NEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEKR
                     RLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVAD
                     KLLVFQGSGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKEW
                     ETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSEW
                     VED"
     gene            complement(37614..39692)
                     /locus_tag="ACG8PO_000168"
     CDS             complement(37614..39692)
                     /locus_tag="ACG8PO_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFQ0205527.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            complement(39705..39980)
                     /locus_tag="ACG8PO_000169"
     CDS             complement(39705..39980)
                     /locus_tag="ACG8PO_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0205528.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            complement(40342..40797)
                     /locus_tag="ACG8PO_000170"
     CDS             complement(40342..40797)
                     /locus_tag="ACG8PO_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0205529.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(40951..42159)
                     /locus_tag="ACG8PO_000171"
     CDS             complement(40951..42159)
                     /locus_tag="ACG8PO_000171"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HFQ0205530.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(42271..42603)
                     /locus_tag="ACG8PO_000172"
     CDS             complement(42271..42603)
                     /locus_tag="ACG8PO_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HFQ0205531.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            42751..43626
                     /locus_tag="ACG8PO_000173"
     CDS             42751..43626
                     /locus_tag="ACG8PO_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFQ0205532.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(43708..44289)
                     /locus_tag="ACG8PO_000174"
     CDS             complement(43708..44289)
                     /locus_tag="ACG8PO_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ReoY family proteolytic degradation factor"
                     /protein_id="HFQ0205533.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(44295..45554)
                     /locus_tag="ACG8PO_000175"
     CDS             complement(44295..45554)
                     /locus_tag="ACG8PO_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFQ0205534.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(45556..46602)
                     /locus_tag="ACG8PO_000176"
     CDS             complement(45556..46602)
                     /locus_tag="ACG8PO_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0205535.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(46837..47112)
                     /locus_tag="ACG8PO_000177"
     CDS             complement(46837..47112)
                     /locus_tag="ACG8PO_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HFQ0205536.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(47355..48665)
                     /gene="der"
                     /locus_tag="ACG8PO_000178"
     CDS             complement(47355..48665)
                     /gene="der"
                     /locus_tag="ACG8PO_000178"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFQ0205537.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(48847..50076)
                     /gene="rpsA"
                     /locus_tag="ACG8PO_000179"
     CDS             complement(48847..50076)
                     /gene="rpsA"
                     /locus_tag="ACG8PO_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFQ0205538.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVSSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(50211..50891)
                     /gene="cmk"
                     /locus_tag="ACG8PO_000180"
     CDS             complement(50211..50891)
                     /gene="cmk"
                     /locus_tag="ACG8PO_000180"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFQ0205539.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(50940..51575)
                     /locus_tag="ACG8PO_000181"
     CDS             complement(50940..51575)
                     /locus_tag="ACG8PO_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAG1386/EF1546 family surface-associated
                     protein"
                     /protein_id="HFQ0205540.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQSGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(51641..53044)
                     /locus_tag="ACG8PO_000182"
     CDS             complement(51641..53044)
                     /locus_tag="ACG8PO_000182"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HFQ0205541.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATEQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            complement(53031..54062)
                     /locus_tag="ACG8PO_000183"
     CDS             complement(53031..54062)
                     /locus_tag="ACG8PO_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0205542.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESAVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            54093..54314
                     /locus_tag="ACG8PO_000184"
     CDS             54093..54314
                     /locus_tag="ACG8PO_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HFQ0205543.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(54479..55216)
                     /locus_tag="ACG8PO_000185"
     CDS             complement(54479..55216)
                     /locus_tag="ACG8PO_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HFQ0205544.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(55369..56709)
                     /locus_tag="ACG8PO_000186"
     CDS             complement(55369..56709)
                     /locus_tag="ACG8PO_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFQ0205545.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(56946..58469)
                     /locus_tag="ACG8PO_000187"
     CDS             complement(56946..58469)
                     /locus_tag="ACG8PO_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HFQ0205546.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(58847..59458)
                     /locus_tag="ACG8PO_000188"
     CDS             complement(58847..59458)
                     /locus_tag="ACG8PO_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFQ0205547.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(59581..59706)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(59788..60504)
                     /locus_tag="ACG8PO_000189"
     CDS             complement(59788..60504)
                     /locus_tag="ACG8PO_000189"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFQ0205548.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(60510..61079)
                     /gene="scpB"
                     /locus_tag="ACG8PO_000190"
     CDS             complement(60510..61079)
                     /gene="scpB"
                     /locus_tag="ACG8PO_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HFQ0205549.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(61063..61833)
                     /locus_tag="ACG8PO_000191"
     CDS             complement(61063..61833)
                     /locus_tag="ACG8PO_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HFQ0205550.1"
                     /translation="MNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDIPIATITEQY
                     MNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFVTDDEELWEEDPREELVTQ
                     LLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKKIDLIRAMQK
                     ILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESPSKSEVVTTF
                     MALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(61830..62201)
                     /locus_tag="ACG8PO_000192"
     CDS             complement(61830..62201)
                     /locus_tag="ACG8PO_000192"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0205551.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(62220..63107)
                     /gene="xerD"
                     /locus_tag="ACG8PO_000193"
     CDS             complement(62220..63107)
                     /gene="xerD"
                     /locus_tag="ACG8PO_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFQ0205552.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
     gene            complement(63203..63676)
                     /locus_tag="ACG8PO_000194"
     CDS             complement(63203..63676)
                     /locus_tag="ACG8PO_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFQ0205553.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEKRNQSV"
     gene            complement(63781..64644)
                     /locus_tag="ACG8PO_000195"
     CDS             complement(63781..64644)
                     /locus_tag="ACG8PO_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor B family protein"
                     /protein_id="HFQ0205554.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYGFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            complement(64840..66939)
                     /locus_tag="ACG8PO_000196"
     CDS             complement(64840..66939)
                     /locus_tag="ACG8PO_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division site-positioning protein MapZ
                     family protein"
                     /protein_id="HFQ0205555.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
BASE COUNT        18233 a        14126 c        11428 g        23310 t
ORIGIN      
        1 ttaaactcaa aaaaatttga ggatacgata taaactttcg gatagagtat aaaataagga
       61 aacgccgcag cgtttccttt ccaactgtag ccgaatataa atttgtatat cacttattta
      121 aaatagctat tttctcccaa agagtttcct tgatcatcga tcaatccttg taatgagcgt
      181 tctaagatac catggctttt tttcgatttt ttttcttctg actgctttgt ttcctctaac
      241 gtttttgttc gattgaatgt tcgtacagac ggctcaccat ttcttttttc ttgatacggc
      301 gtcggttctg tatcgaacaa cagataagag tcatccgttt ttttcatcga ttcgattaaa
      361 tgatcctcag aaattttgct ttctctaggg tctgggataa tcgatgcggg tacatattta
      421 ggaacaaaat aagaacgctc ccgcaccttc gtgtcaacag atgcgtttcg tgtagtatgc
      481 ttggactctg tccttgtttc actttgtctg acagaacgag aggaatcacc aaaaagtttt
      541 tttcgtcctg tcgatgtgat ttctgattgg taatggccgg tataatccgg taattttcct
      601 ttatgctttt ccaaattttc cttacgcgga gctgacttca atccatttgt tgcacgacgc
      661 ttgcgacgga ttttctcggt gttcgtgttc gtgaaatgaa attctttcat ctgttcaaac
      721 ggggactgat tctgttcagt caaaagatct tcttgtccgt caaataatac tcgcttcttg
      781 tcgttggtta attttacgcc taactcatca tcgtacgcag gaaaacggaa atgttttcgt
      841 ctgatttctt tcattcaaac catccttctc aaaaatggaa cttttctcaa taatagcaca
      901 gattcattgt tttcagtagc aatgcgctga ttattttaag aaaatagcga tgaatttcat
      961 aaatatatat atagtaataa gacttaattc gttgtctctc tgataactag ttcaggaaaa
     1021 aaacgacgat aaccttgact cgcccctttt tcttctattt tttttaacaa catttctcca
     1081 catacttcac ccatacgtat aatatcttgc ttgatcgttg tcaatttagg tgaactgatt
     1141 tggtctaaaa ataccccatc aaatccaatc acaccaaatt cttcgggcaa gtgtcctcca
     1201 gcatttataa tccctcttac tgctcctagt gctagccgat ctgaactaca tacaaaacaa
     1261 gttttatctg taaaatctgc ccagttttct gtcatatatc tttctacttc tgtcgaatga
     1321 tttggaaacc gcaagatttt actaggaaga tcaacttcct tcatcacaga taaataacca
     1381 gtttcccttg aaatttcaaa aggctcgtcc acttcgatac cgatataaat aatcgtttga
     1441 taatctcttt ctaaggcata ttgcgttgct tttgctgtac catattcatt atcgctgtct
     1501 acataatcat atccttcaga attttcccca aacaagacaa ctggtttttc caaacggtcg
     1561 atccaatcga agtctttttt tctgacacct gtgatgatgt atccatcaca agaaccgacg
     1621 tcgaaggcat catttgtaac taattggaga gaataatgcg ctttaccgat ccgtttagcg
     1681 attcccatca gcaaattcat gtaatagggc tcagttgtgt cgatttcttc cagtataaat
     1741 aatttgatga tctgcgaccg attcgtgaca agtgcttttg cagcaatatt tggacggtaa
     1801 tccagttcag ccattgctga gaaaactaac tctttcaatt cgtcagtcac ctgttccgga
     1861 tgattgatga ctcgagagac cgtcattttt gaaacattgg cacgttttgc aacatctgct
     1921 aaagtgggca tgctctcttc ctttctaaca tgttgtcatt cttactatca attataccaa
     1981 attttcctaa cagagtttat tatagcccaa aataaaaaac ggaaaatcct ctatcgtcat
     2041 gagtgatttt ccgtttcaat tatttatact tttttatcaa attttttgct aaaaaagtgg
     2101 aagcttcttc cattagtggt atttgtacca tatgtcgctg tgtttttgag gaacgaaaaa
     2161 caatttcttt tccgtaagac ttatgccgat ttttttctac aaatcgttcc gtttgtatat
     2221 aagggatttt ttcgtcagct tgtccatgcc aaaagaacag aggacgtcca gctaactttt
     2281 ccggctgcaa tgacaaatca tagctcttaa cccatgaaat caaatcaaaa tagtcttttg
     2341 gtacgacata attatattcc gatgcgcgcc ttacgatttc ttttccatat tccaaaggtg
     2401 caggggaacc catcaaacac acgcccgcta ctatatgagg atgtttagcc aacaaggcgc
     2461 atgttgtcat tccccccatc gaaaccccgc caactgcaag ctgatcatgt agaagacctc
     2521 ttttttctaa atgcttgata agtgtgtcaa attcaaacag atttccgtag atactatccc
     2581 aaaatgtata ggatggaata gaagaaaccg gctgtttgcg ctccccatga ttcatcgcat
     2641 ctggcaggat cacacgaatg ccctgctgcg caatttttct tccttgcgtc aaaactaact
     2701 ccttacttgt ctgccatccg tgataataga tgacaacggg aatacgttcc tctcttaatt
     2761 ctttcagaac aacttctaac actggaatat gatggacaga acgtttcaat ataattggat
     2821 tcaattgtta ctcacctatt ttcattaaat ttcctgatcg ccatagcaac catgctccaa
     2881 cagttgtctg gaacaggttc agtgtaccaa ataatataaa tgcaggactg aatggctcgg
     2941 gaaataagca cagagaacaa agtcccacca ctcctaatgc tagcgaaagg agtggataaa
     3001 tagaaaaatt caaaaaactg gcaacgctac cgaaaatatt accaataaac agtatcaaaa
     3061 agaaaaaaac gcttaaaatt aaaccatctg tcagcgacat ctgaaaaaca atgttctcta
     3121 cctcaattgg ctgttgaatc gaatagacac ctaagtatat actgagaagg agacttagca
     3181 cgtggctggc acaaaacaca cgattgaccg ttttcatttt aatcacctaa cctatcttta
     3241 taattctatt ataatcattt tatcattttt ttgtatgtat aaagtttcaa tttttcttca
     3301 cttacgattt tttttgatat tgttgttata taaatgaaag gagttttaca aatgattgat
     3361 ccaaaaatgt tggggatgat cttcctcatt aattttgctt atatcactct gaacacgtta
     3421 cgtttcatgt tgacaatgaa aggctatcga ctgattgctc cacttgtcag tatggtcgag
     3481 atcacgattt atatcgtcgg cttatccctt gttttagatc gtttagataa tccactgaat
     3541 ttatttgtct atgcgttagg ttacgcagta ggtatcagtg tcggaatttt gattgaagac
     3601 aaattggcat taggatacat catggtcaca acgattttgc cagcaaatag cgcagaagat
     3661 aaaagtttgc cgggtatact gagagaacac ggttatggtg tgacacagac ttctgcaatg
     3721 gggctggaag gtgagcggct agttctcgaa atactttctc ctagaaaaag cgaacgtgaa
     3781 ctgtatcgcc tgatcaaaga aatcgaagag cgtgctttta tcatttctta tgagccaaaa
     3841 tatatttcag gtggtttctg gactaaaaaa gtgagaaaac gacaaggaaa ataactcaca
     3901 aacttaaaaa ctgtctaata tctaaattaa attttaaatg ttagacagtt tttatttatt
     3961 attttgatta aaaaacatcg acatatactc atcaatttgt tattatactt tatatactta
     4021 tcaatttaat ttggagatat tatggaaatt caacgtagaa ccactaattt tttacattta
     4081 ggctcttttt ttgcaacttt gacgatctta ttttttctat gttattcata ccttaagacc
     4141 tcacatgcgc cttctagtgc attaggctgg ttgcttttgg atcattctga tatcacagaa
     4201 gccgaagaaa atccgttttt ttttgatttt gtcttctctt tgcggcttac tgttttctgt
     4261 ttatttcggt gcctattttt ttcatcgaca tcctggtacg ttaaacgaag cttcaagcgt
     4321 attgattgca tttaaaacgt tttctttttt agcaattctt ttttattcgt ttaatcacaa
     4381 tacgcttttc ttctttatca tgaatggttt agtcgtgatt ttatccattt ttacttattt
     4441 tattttttca ctcattttta gccaattgaa atgtccgctc ttctttcgat tagttcctgt
     4501 cacctgtttt atttacagct tgttttcctt ttttgtcctt gctttaatgc cactgaccaa
     4561 cccttcgacg atccaagtcg caggaaatct gttggatatg ctcttcatcg tcagtctagg
     4621 tgtatttatg tggcaaagaa aaaacaaaca aaataaaaag cattttgaag cctgactatt
     4681 acgacttcat aaatgctttt ttagttttaa acacttcaat ctaaaagaat aacccaagat
     4741 tggataaatt attaatgact cgccattcgt ttcttagtga ttctggatta tcaaatggac
     4801 ttcctaatct aatcagctag ttcttcccat aaaatgccat acttgactga atcataatat
     4861 tgattattta aaaatctaac ttttcttatc actacttcct ttgtcattcc acatttctca
     4921 ccattttttt gcattccttt gtttccagac catgtagtga atctaatagg tggtagatag
     4981 gcaaaatact caaataattc ttttaaccat aaaggaagag aaaggctcct cctgcaagag
     5041 tcagtataat ttttgcttac tgctctctta ggttttactc actaaaactg ttccatattc
     5101 tttgatataa gaaaatgcat gcggtataaa tttcttgctc agctcagaaa agatttgtcg
     5161 tttaagaaag gccatttcct ccattgtcaa tgaaggatcc aatgttggta attcaatctc
     5221 aaaattgttt ttttcgacaa taggtaattc taatgtgaat gattcttctt cctttatagt
     5281 attcgtcaca agtagagact gatggaaaat actaggccgg cgatttttat atttattctt
     5341 acgtatcctg tcaacgataa cttctatttg atcaaaaaag tacgattcat ttacgtctcc
     5401 aataagttcg gctaattctt tgaatgcctt cgttacagga agctgattct tctgtacaaa
     5461 aaagtaatta gccaaagtaa gcgggaagtg ttctatagag gaaagtgtga taaaacggta
     5521 cttctcattt cctgcagttg tcctcagcaa ttctgtctct cggctgtttc ttactccata
     5581 aaaatacgct tctttccgtt catcttcgaa acaatacgcc caaataactt ctcccatcgc
     5641 taatttttgt tcatcaacag agagtctctt caattgttca gctatggttt cagcagccga
     5701 agtggcttgt tgcaagtaat ctttaggatc aaatattgga aagttcaaat gatatcttcg
     5761 ttcttccctc ttgattaatc ctgcatcgat caataaatca agaaaatggg aaaatttatt
     5821 ttgcgaaaat tcttttttca attctcttag aatcgtttct tgttctcgat gttcatatag
     5881 ataatcggtc aatgaagaaa aaagcggatc atcggtcagt ttttgataaa agttttcctt
     5941 attttttgaa taatagttca tatatgttct cttttctatc cgttttattt tacttaaaag
     6001 gcttttacgt aaaaaggagg aagaacaaga gtcataattg actgttattc tcctccattt
     6061 cttcggcctt tagtatgttc tatgtttctt tttatatcaa tcgcttatcc attcattttg
     6121 aatgcttcat actaccattt gattagctaa atacagccgt caattgaggt acgacttgtt
     6181 ttttacgtga aacgacacct ttaagcaaag cacggttgtt ggtcaaagtt gtttggaaag
     6241 cttcttctac tttttctttt cctttaccag caacaagtaa ttctgaatca ctgtctaaga
     6301 tgtttgtgac aactaatacg aacagatcgt agccatttgc tttcatttca tcttccattg
     6361 ctgcttctag ctctgcttgt cgatccatca cttcattaag gtccactgta ttgacttgag
     6421 cgatacgaac gttggaactg cccattggga aactttttgc atccaaatca agaagaaccg
     6481 cagctgattt atcacttaga tttgttcctg ctttcaacat ttcaagtcca taagtgttta
     6541 gatcgacttc agcgattgct gcaagttctt ttgcagctgc ttcatcttca ggcgtacatg
     6601 ttggtgattt gaacaacaat gtatctgaaa tgatagctga gagcatgatt cctgcgattt
     6661 ctttaggaat tgttacttgg ttttctttat atagttttaa aataatcgta cttgtacatc
     6721 caactggttc tgcacgatag tacaatggat tagctgtttc aaagtttgca atacgatggt
     6781 ggtctactac agctaaaata tttacttcgc tgatatctga aatactttgt tggaattcat
     6841 tatgatccac aagcatgaca tctgcatttt ctgctttagc tgactctact actcgtggtg
     6901 cagatagctg aaagtggtct aatgcgtatt gtgtttcttc attaggtgta cctagagcaa
     6961 ctgcttctgt ttctttacct aattcatttt gcaagtgtga aaacgcaatg gctgccccga
     7021 ttgcatctgt atctggattt tggtgaccaa aaactaaaat ttttgacatg tcattcgctc
     7081 ctttgtgatt tctcctattt aataatagca aaaaaacgga aagaagcaat acttatcttc
     7141 tttccgatgc gacctatcgc gttaaatatt ttgtatactc ttctgtatga agcaatcgtt
     7201 tagcattttc gacacgttct tttgtaggtg gttcgatccc ttctaatgga tatttcaaac
     7261 caagtgtttc ccacttgtat tttcccatcg tatgataagg aagaatctct actctgtcta
     7321 catttgacaa ggtttgaata aagttgtcta gtcggatcaa atattcatca taatcacttc
     7381 gctcaggaac tagtacatgg cgaatccaaa ccggtttgtt gatttttgac aaatattgag
     7441 ccatttctaa aatattttca ttgtcccaat gcgttagttt tttatgctct tcattatcga
     7501 tgtgtttgat gtcaaataac agtaaatctg tatacttcat caattcttga aaacgtgaaa
     7561 agaaaggttc ttcatacgta aaaggttttc cgcatgtatc caatgtagta tgcatcttcc
     7621 gctcttttgc ttttttaaat agttcgatca aaaaatcgat ctgcaataga ggttctccac
     7681 cactaacagt aataccacct ttatctcccc agtaaggtct gaagcgttct gctttatcta
     7741 aaagttcatc tgctgtgtat tctttccctc cgccaatatt ccaagtatct ggattgtgac
     7801 aaaattggca acgcatgcgg catccttgca taaacacgac aaagcgcagt ccaggtccgt
     7861 ctactgaacc gaaagtttcg atagaatgaa catagccgat tgttttttct tccatttaag
     7921 ttcacatctt ttctttgatt tcattttaag tacaagttag ctgtcgaagc gaagctttct
     7981 gaacaaaatt tttcgcaatt gccgcttcgt tatgtgtaaa atttacaggg acggaacata
     8041 caagtcctgc ccctgtaaat cgtttctttc aaataagcat actgattatt cgattttgat
     8101 gcgatggcta ttcaacgtcc tttcgacgct aagtcgaaaa acttttttca cgaacttttt
     8161 acattctgtc gtgtgaagtt ctcatcaaga catctaattg ttgttctctt gtcaagtcac
     8221 ggaatttaac tgcataacca gatacccgga tcgtcaagtt cggatatttc attggatttt
     8281 ccattgcatc acgcaataaa tcattactga atacattgac attcaaatga taagctcctt
     8341 ttgagaagta tccatctaag acattacgca agttttcgat tcttgtatct tgttcttttc
     8401 caagacctga aggattgatt gtttgggtat ttgaaattcc atccaatgca cagccgtaat
     8461 tcattttagc tgttgagttc aggctagcta ataacccact ttgttccgca ttgtaacttg
     8521 gatttgctcc tggcgcaagt ggttttccag cttcacggcc atcaggagta tttcctgtat
     8581 ttttaccgta aacaacattt gaagtaatcg tcaataatga agttgttggt tttgagttgc
     8641 ggtaagtttt atgacgtttg atttgtccca tgaagtattc caagatccat gaaactaatt
     8701 catctgcacg gtcatcatca tttccgtatt tagggaagtc gccatccact tcaaaatcaa
     8761 ctgctattcc tttttcatta cggattgctt ttacttttgc atatttgatc gctgacaagc
     8821 tgtctgccgc atgagagatc ccagcaatac ctgtagcaaa tgtgcgttgc aagtcagaat
     8881 ccatcaaagc cagctgagct gcttcataag aatatttatc atgcatataa tgaatgatgt
     8941 tcaacgtatt gacatatagt tctgctaacc aatccatcat attcatgaag ttatgtttca
     9001 cttcttcaaa gtctagtatt tctgatgtga ttggacgata ttctggtcca acttgggcgc
     9061 cagtcacttc atcgactccg ccattgatgg catataatag tgttttagcc aagttagcac
     9121 gagcgccaaa gaattgcatg tcttttccga caactgttcc tgatacacag caagcaatcg
     9181 cacaatcatc agaaccccat tgttcacgaa gctgattatc attttcgaat tgaatcgaag
     9241 aactttcgat agcgattctg ctagcatatg ttctgaatcc ttctggaagg tgttcagaat
     9301 acaaaacagt caagtttggt tctggtgaag gtcccatatt tgttaatgta tgaagcagac
     9361 ggaaatcatt ctttgtaact aatgaacgac catctaatcc catacctgca atggataaag
     9421 tagcccagat agggaatcct gagaataatt ggttatattc aggtgtacgc gcgaatttga
     9481 ccatacgtaa tttcattact aagtggtcga tcaattcttg tgcttctttt tctgtgatta
     9541 ctccattatc caaatcacgt tggatataga tatcaaggaa tgctgagata cgtccgattg
     9601 acatcgcagc accattttgt gatttgattg ctgctaaata tccgaagtat agccattgga
     9661 cagcttcttt tgcatttgca gctggtttag aaatatcata gccatatttc gcagccattt
     9721 ctttcatttg cgctaatgca cgatattgtt cagtgatttc ttcacgtaaa cgaatcactt
     9781 cttcactcat tgtatgataa ccagtcatat catgatcttt tttcttttgt tccatcaaat
     9841 agtcgattcc atacaatgcc acacgacgat agtcaccgat gatacgtccg cgaccgtaag
     9901 cgtctggaag ccctgtaatg attttgtttt tacgagcagc acgcatttca gcagtataag
     9961 cgtcaaatac accttgatta tgtgttttgc gccagtcagt gaaaatatgt gtcaattcat
    10021 cgtttggtgt atagccattg ctttttagcg cattgttagc catattgatt ccgccgaacg
    10081 gcatgaatgc ttgtttcaaa ggtttgtctg tctgtagacc gacgattttt tccaaatctt
    10141 tatccaaata gcctgcatcg tgtgaagtaa tagttgatac gatttctgtg tccatatcat
    10201 agacaccgtt acgctcgaat tggatatcat ttaacttttg caattcatcc cataattttt
    10261 ctgttgcttc tgttgggcct tccaaaaagc tgtcatcacc gcgatattca gtgtagttat
    10321 tttgaataaa gtctctagtg tctacgcctt ctttccattt ggtaccttta aatccattcc
    10381 attgctccat tgtatggacc tcctaaattt ctgttttatg taacagtttt tacgtgatta
    10441 gtataacaca gcaccctatc tttgcaaggg ttttcttgtt ttttatttaa caatttttat
    10501 gttttattta catatattta aatcaacgtt gacataccaa catttaagca ttgtgaacac
    10561 tttcatcttt ttttgcaaac gtataaggat ttattaaaaa tgaatgaaaa agagacgcca
    10621 cctgtattaa tacagatggc gtctcttcat taaaatacta caacagttac gactcgatga
    10681 aaatctcagg agcatcgata acttcgatga cttctccacc ttttttctca tccatcgcaa
    10741 atgatccatt cgatgttcga tcacctatag ggaaatcagt tgggtcaatc gttaactgct
    10801 gccctttttg attcatcaag gttatttctt ttgatcttcc ttccgacata tagataatac
    10861 ggtgcggatt ttttttcagc tcacgcatga ccgtcactcc ccgcttcgca cgtgaagttt
    10921 ggctgatttc ttgagcaagc atgcgtttaa cagctcctcg ctgagtaacg attacgatcg
    10981 gcgtatcacc ttctgctaac acaagcagcc cgttgataat cctatctcca tctttcaaat
    11041 tcatagactt cactccagct gcctttgctc caacaacggg aacatctgtt aaaggatagc
    11101 gcaaacccat tccttgatgc gttactaaga aaacatctaa gcctggctga tcttctttca
    11161 gatacacagc aatcagttca tcttcttccg cctttagctt catacagctg agcggacggc
    11221 tcttataggt gcgccatggt tcgaactctg tcattcgtgt ttgcttgatc aatccattct
    11281 tgctgatgaa tacaaatgtt ttctctgctt gaattttttg atatgggaaa acagctaaaa
    11341 tcgattcgtc tacagacaga ttggagattg tttgagaaat gtgctcacca gcatctttcc
    11401 atttcaaatc gggtagttca tggacagggc ggtagatcac atttgcttta tttgtgatca
    11461 gaagaacatg gtccaatgta ctcaattcac ctgagtatag caagtagtcg ccttctcgca
    11521 tcccgatttc ttctggtttg gaagcactat aagaacgaag gcttgttcgc ttcacatagc
    11581 cttctcgcgt cacactgaca attacgtctt cttgtgccac taagacttgc gtatcaattt
    11641 tgatttcttc gatttcgtct tcgattgtcg ttagacgagc ggaagaataa ttttttttca
    11701 cttctcgcaa ttcttttttc ataacagaga aaagctcttt gtcatttgaa aggattttat
    11761 tcaactctgt gatttgcgca atcaagcttt cggattcctt ttgaagctct gtgatatccg
    11821 tgttcgtcaa acggtataat tgcaatgtga caattgcttc tgcttgttct tctgtaaatt
    11881 gaaagacgtc gactaaattc tttttggcgt cttttttgtc tttactttca cgaatcgttg
    11941 cgattacttc atccagaatc gataaagctt tcatcaatcc ttctacgata tgctgtcttt
    12001 tttgagcttt ggctaaatcg aattgactac gttttgttat gacttgcttt cgatgctcga
    12061 tataacttct taaaatgtct ttcagtccaa cttgatgcgg agtcatatgg tcgatagcga
    12121 ccatattgaa attgtaattg atctgtagct cagtgttttt gaacaaataa ttcaaaattc
    12181 cttgagcatt ggcgtctttt ttcaattcga cgacaatttg cagacctgtt ctatcacttt
    12241 catctcgtac ttcagctatt ccatcgattt tcttattcaa acggatctca tccatttttt
    12301 tcaccaaggt cgctttattt acttcatatg ggatttctgt gatcacgatt tgctgtttat
    12361 tacccttcat tgcctctatc tttgttttcg aacgaagaat aacttttcct cgtcctgttt
    12421 cataagcttt tttgatttca tcttttcctt gcaagatccc tcctgttgga aagtcaggtc
    12481 caggaatata ttccatcaat ttttcaaggc tcgcttgtgg atgatcgatc atatagattg
    12541 tgccatcgat cacttcagct aagttatgag tcgggatttc tgttgcatat ccagcagata
    12601 tacctgtcga tccattgacg agaaggtttg ggtatcgagc tggaaggact gttggttctt
    12661 tttctgtatc atcaaaattc cacacgagat cgactgtttc tttctcgata tctgctagca
    12721 tctctccact taacttggat aatctggctt cagtataacg catcgcagca ggtggatcac
    12781 cgtccatact cccgttattc ccgtgcattt cgatcagtac ttcccgcaac ttccagtctt
    12841 ggctcatgcg gaccatcgct tcataaatac tgatgtcacc atgaggatga taattcccca
    12901 tgatatttcc gacagatttt gctgatttac ggaatccttt gtcatacgta ttgccatctt
    12961 tgttcatggc aaataatatc cggcgttgca ctggcttcaa tccatcacgg atatcaggca
    13021 gtgcacgttc ttggataata tatttggaat aacgtccgaa gcggtcaccc atcacttctt
    13081 ctaaagttaa ttcttgtact tcttgtcgat tttccatcag gtgacctcct attctacttc
    13141 aaataactga ttgttttcat ttttatctgc tcgttcttca tctaataaat cattagaaac
    13201 tgaaggagag atttcagtat cctctttttt atctaagata ctgccatctt cttcgagcgt
    13261 aaattgcacg tgattttcga tccattttct tcgaggttct actttgtcac ccatcagtgt
    13321 ggttactcga cgttcagctt gcgcagcatc atcgatacgg acgcggatca acgtacggga
    13381 agtcggatcc atcgttgttt cccacaattg ttccgcattc atctctccaa gtcctttata
    13441 tcgctgcagc atatatcctt tgccgacttt tttaatcatt gctgccaatt cgtcatctgt
    13501 ccacgcatat tcaatgactt gcttctttcc ttgtcctttg gatactttat ataaaggcgg
    13561 taacgcgata tatactttcc cagcttcaat cagcggtttc atataacgat aaaagaaagt
    13621 aagcagtaat acttgaatat gggcgccgtc tgtatccgca tcggtcatga tgattacttt
    13681 gtcgtaatta cagtcttcga tagaaaactc aggaccgact cctgcaccaa tcgtatagat
    13741 catcgtattg atttcttcat ttttcaagat atcttgcatt ttagcttttt cggtattgat
    13801 gacttttcct cgtaaaggta gaattgcttg gaactttctg tctcttcctt gtttagcaga
    13861 tccgccagca gaatcccctt cgactaaata caattcattt ttcttagggt ttcgggattg
    13921 tgcgggtgtc aattttcctg aaagaagaga ttcgcctttt ttacgcttct tcccatttcg
    13981 gctttcttct ctggctttac gtgctgcttc tctcgcttct ctggctttga ttgctttacg
    14041 gaccaacatc tggctcattt cgctattttc ctgcaaataa aagcccattt gttcactgat
    14101 gacgttatct actacggtac gagcaactgg cgtaccaagt ttttcttttg tttgcccttc
    14161 aaattgcaga agattttcag gtacacggat agaaagtaca gcagctaacc cttctctgaa
    14221 atcactacct tccagattct tgtcccgttc tttcagcaaa ccaacttttc tcgcatattc
    14281 attataagat ttcgtcatcg ccgctttcat accagcttca tgggttcccc catcttttgt
    14341 ccgaacgtta ttgacaaaag ataaaacatt ttctgaataa ccgtcattgt attgataagc
    14401 gacctctact tcgatacctt ctttttctcc tgaaaaataa acaactggtg tcaatgtatc
    14461 tttttcttca ttcagataat caacaaattc tttgattcct tcttcgtaat gaaaaatttc
    14521 cttgactggt tcttctccac gaagatcaga aagagagatt ttaacgcctt ttagaagaaa
    14581 ggcagattct cttaaccgtt cggctaaaat ttcatatgaa aatttcgttg tactgaatat
    14641 tgtatcatct ggtaaaaaat ggacagtcgt cccgtttgct ttttttgttt ttcctatctt
    14701 tttcaaagta ccgtcaggtt taccgccgtt tttaaatttc tgctgatact ctacgccatc
    14761 tctaacgatc gttacaatta gccatttcga taaggcattt accacactgg ctcctacacc
    14821 gtgcagtcca ccagaagttt tgtaccctcc ttgaccaaac tttccgccag catgcagcac
    14881 agtaaaaatc acttcaactg ttggtattcc ggaagcatgc attcctacag gcatcccacg
    14941 tccgctatct gcaacagtta tactattgtc ttcatggatg gtcacatcaa tctcactgcc
    15001 ataacctgat aatgcttcgt caactgcatt atcgacgatt tcatagacta aatgatgcaa
    15061 gcctcggctg tcagtggatc caatgtacat ccctggtctt tttctaactg cctctaatcc
    15121 ttccaatact tggatggacg agtcattgta ttcgttgttt actttttttg ccaaggcaaa
    15181 cgctccttca ataatacttt gcggcaaaac cgcaccacga ttatcatact agaaaaggtc
    15241 gtgaaaaaaa agagggtaga cgagaaatca attttaatta tctcctaacc gctttttcga
    15301 cattcccctc tatcgatcat ctcttctttg tggtcatttt tatttttggt tttctttcaa
    15361 cttaccaagt tcaatcttaa tacaacgatc cattacgata tctgtacgtc ctgcttttct
    15421 tagcatctca gctgcttctt tgttttcaat ccctaattgt gcccagaaca ctttagcatc
    15481 agttttcaaa aaatcacctg ctatttcagg taaaaattca ctccgtctaa atacatctac
    15541 gatatcgatt ggatcatcga tttctgtcaa attcgcatag actttctcgc ctagaatttc
    15601 ttcaccagcc aataatggat ttactggtat gattttatat ccattgtctt gcaatacctg
    15661 tgcaaccatg taacttgttc gttctggttt attgctcaag cctacgacgg caatattttt
    15721 tgcttcttcc aaatagttga aaatcgtttc ttgtgttgga ttttttacag acatttctgc
    15781 caccccttgc ttttcttttt attccatcta ttatacttgt atctttcatt ttttcaattg
    15841 ttcctgttac ctattcatgc taaaataaag gatagttttc aacttgaaag agggtaaaaa
    15901 atgaaattga tcttattact tattttagca tatcttttag gttctattcc ttccggtgtc
    15961 tggattggca aattgttttt caaaaaagat atccgacaac acgggagcgg taatactgga
    16021 acgaccaata catttcgtgt attaggcaaa acagccggga ttgttgtcct gcttatggat
    16081 attttaaaag gtacattggc tacttgtttg ccgatgatct tccacctttc tgggattaac
    16141 cctctttggt ttggggtatg cgccattctt ggccatacat ttccgatttt tgctgggttc
    16201 aaaggaggaa aagctgttgc gacaagtgca ggaatgcttt taggatttaa tcctgtattt
    16261 tttgtttatt cttgtatcat ctttatcgtt agtttatttt gtacaagtat ggtgagtttg
    16321 agcagtatga tcagtgcagt attgatcact ttatctacga tcgtccttcc ttatgtagct
    16381 cctgtgattt tggctaagcc caattggtta ctgacgatca ttgccttttt agtttcttcc
    16441 tttatctttt atcggcataa agacaatatc aagcgtatca gaaaccatac tgaaagtcga
    16501 atcccgtttg gtttgaatag cagcaagaaa aaatcctgat actttttcat tccaaagaga
    16561 taaaacggga gtgtgacaaa tcgatgtcac actcccttat tacttacagg atagcttttt
    16621 tacacacctt ggtaaacggt gttccctgct aacttctcgg tcacaacctc ttacttcact
    16681 gtgatggaat attttgtctt gaagatttca tgggcaccta gttgattgat ccctactttt
    16741 tccatcaagt ttccactact gttcaccgtg tcagcgattc cgcaccacgg ttcgatacaa
    16801 acgaaaggcg cttcttttgg ataaggagac cagatcccta cataaggcat atctttatag
    16861 cttaaagtta cgctatggga agatttttca cttcgaatgg cgtacgcatt tagacccctt
    16921 gtttcaaaaa ccaaggcatc ttgctcaaaa agttcacgat tcaaagccag attcgtattt
    16981 gtctgaccta acgtcttttg atccatatcg atgaatgggc ctttcaatgg taggcgtaat
    17041 cttgatttca atggagaaaa tgccagatag aaatcttcaa atgacaatcc ttcttcaaga
    17101 ggtacattga aagcaggatg tccaccgata gaaaagaaca tttctttttc accggtattt
    17161 gccacttgat aatgaaccgt gatgccgtct cctcctaatt catagctgat cactaattca
    17221 aaatcaaatg gatacttttc taatgtctcc ggcgtactcc ttaagatgaa actagccatt
    17281 tcttgtccat gttcaatgac ttcaaaatcc atatcccgag caaatccgtg ttgccccatt
    17341 tgatatgttt tcccttgata aacatactga tcatctttta aacgtccaac aaaagggaat
    17401 aaaaccggtg catgtctgcc ccaatacgct ggatcacctt gccagatata ctctaaacca
    17461 tgttcttttg atttcagact gatcaattcc gcaccgtgtt cagcgatttc ggcgatcagc
    17521 tggtcatttt gaataataac agtcattgcc attcctccaa cttcgactta ctttttaaaa
    17581 tgggaaggct ggaacaaaca tttctatctg ccccagtccc cttacttcta ataactgtgt
    17641 tttgctaact cgtctttaaa acgggaattc aatactttta aataagttcc cttcatgcct
    17701 aacgaacgag attcaatgat tccagctgat tccaattttc gtaaagcatt gacgatcaca
    17761 gaacgagtga tgccgatact atcggcaata ttagaagcag tcagacgacc ttcatctcca
    17821 tccaatgctt caaagatagc ttgtacagcc ttcaattcgc tataagataa tgtgttgatt
    17881 gccatttgga cagccgttgc actccgtaca tctgcttcga tgcttcgaga cttctgataa
    17941 aggatctgca taccgacaac cgtagctcca tattctgcta agactagatc gtcatctgca
    18001 aacgattgtt ccatacgaga taagataatc gtacccaaac gttcgccagc tccaaagatc
    18061 ggaacgacag tcgtcagacc aaatggatat ttctctcttg actctaccgg aaatgcagta
    18121 atatcactag taattgggat attagcttca gttttcatca actgtgccac tgtatcagta
    18181 taagattcag gaaattgttt ctcaacaaac attgttttga ttcgttcatt gttcacatcg
    18241 tgacgttcat tataacctag taatactccg tcacttccta tgatataggt gttgctgtca
    18301 agaataccac ctagtgtgac agccatttta tcgtaaggca aatctgcttg catgtcaaaa
    18361 gtattctttt gctgcaaaag tgtgtttatc ttgcgggttt tcgttagcaa gtctgtcatt
    18421 gtcgtcctcc gattttttat aaaatatagc gacttaaatc ttcatttgcc gcaattgagc
    18481 caagtttgtc atccacatat gcttctgtga tcgttatttc tcccatttgc atatctggtg
    18541 cttcaaataa tagatcttct agtagttttt ccaaaatggt atgcagtctg cgtgcgccga
    18601 tattatctgt gtcacggttg acatcgaaag cgatttgagc gatccgttca attgcttctt
    18661 ttgtaaacgt aactgacaca ttttctgtac cgatcaacgc gacgtattgt tttactaaag
    18721 cattatttgg ttcggtcaga atcttcacaa aatcttcagc tgtcaaatca tctaattcta
    18781 cccgtatcgg gaagcgtcct tgaagttctg gaattagatc acttggttta gacaaatgga
    18841 aagctcctga agcaatgaat aggacgtgat ctgtttggat gatcccgtat tttgtgttta
    18901 cttgtgaacc ttcgacaatt ggcaatatgt ctctttggac gccttctctg gatacttctc
    18961 cagattgctg tgatttagaa gtgatcttat cgatttcatc gataaaaata attccacttt
    19021 cttccgctaa tttgattgcc tcactatgga tatcagcatc cttgactaat tttgctgatt
    19081 cttccttgat caataactct aaagcttctt tgactgttac cgtccgttcc acttttttct
    19141 tcggtgtcaa agcacctaga gtttcattta ggtcgatccc catttgctcc aatccattgt
    19201 tcatcattgg tgcaggtttc ttttggtctt caacttcaat ggttacttca cgactatcca
    19261 gtaaaccctt ttgtagttgt tccaagactg tacggcgatt gattcgaata tcttctgtca
    19321 cttcttcttg cggttcagcg gtattttgct gactagcatt aaaaagattc atcatttgtt
    19381 caaattgatt agtggattgt ttctgttctt ttttgatacc aggtaccaag atttttacca
    19441 aacgtttttc tgcatttttt ttagcacttg tgtatacacg gctgtactgc tctttttcta
    19501 cgatttggat cgcattttct actaaatccc gaaccattga ttctacatct cttccgacat
    19561 aaccgacttc agtaaatttt gttgcttcga ctttgacaaa tggtgcattg acgatttttg
    19621 ccaaacgtcg tgcgatttct gttttaccga cccctgtagg tccgatcatc agcatatttt
    19681 ttggtgtaat atcttgttgc attttttcat ctaactgcaa acggcgatag cggttacgca
    19741 aagcaacggc aacagatttt tttgctgcgt gctgtcctac gatatattca tctaattcac
    19801 ttacgatttg tcttggtgtt tgttttaatt catacatatc tactcacctc ttacagttct
    19861 tctacgataa tattatgatt agtaaagata cagatatctg cggcaatatt taacgcattt
    19921 tctgcaattt ctttcgcact catgtcgttt tgagcatagt ttttcattgc tcgggctgca
    19981 gccaacgcaa aattccctcc tgatccgata gctaagatac catcatctgg tgtgataact
    20041 tctcctgtac ctgaaacaag aagcatttct ttatcattca ttacgatcag catcgcttca
    20101 agtttttgca tagattgctg tgtccgccat tcttgagcta attcgacagc tgcgcgttgt
    20161 agattcccgt tatattcgtt tagtttgcct tcaaattttt cttctaaagt aaacgcatct
    20221 gcaacacttc cggcgaaacc tacaacaact tctccattgt agatacgtcg tactttcttt
    20281 gcagtgcctt tcatgacaac agattcaccc attgttactt ggccatctcc agccatagca
    20341 aatttcccgt ctttttcgac ggcacagata gtggttgaat gaaatggtga ttcgaccatg
    20401 tttgtttcct cctaaatttg ttcttttcct tatttttacg ctcttggatg gaaagaacga
    20461 tagttttttt gtaaactttc ttttgttaca tgggcataga tctgggtagt cgataaattg
    20521 gcatgtccta gcaattcttg gactgtccgc aaatctgccc cattatttaa taaatgagta
    20581 gcaaatgtat ggcgcagcat gtgaggatgg atatcactat ccaaactgct ttttttgatc
    20641 atttgattca agacatattc aattccagta ggcgtgattg gttttccatg atgattcaca
    20701 aaaacgatat catgctcttc gtgatatttt gtcatcaata cctggcggcc ttccctgaaa
    20761 tattcttgca gagcatcttg agcaaaagaa cctagaggag catatctctc tttgttccct
    20821 ttaccatgga tcaacatgac ctctgaatca aaatcaatgt ctgataccga caaactgcta
    20881 cactcactta aacggatgcc tgacccgtaa aggacttcca gtaaagcgcg gttcctcaaa
    20941 tccaaaggac tatctcctgc tacgctgtca aaaagtgctt gcatttcatt ttcataaaaa
    21001 aaacgtggca gtctcaaatt tttctttttg agatgaacgt aagaaaatgg attttctttt
    21061 atcacttctt ctttgagaag atactgataa aacgaacgta ggctggctat cttgcgactg
    21121 atagaatttc tgctatagct aggacgatga tcagctaatg cactcaaata gacacgaacc
    21181 gtaaaatatt ccgccttcag cagatcagcg tctccagatt cctccaaaaa atgtttgaaa
    21241 tggagaatgt cttcttcata tgcttctttc gttttttctg aatatcctct ttcgacaatc
    21301 aaataacgca aaaatttttg ttcccacgtg gcttctgtca ttttctcacc ttccttttgt
    21361 cccagagaat aatttagcac agcgtttata aaaagacaaa taaattgtca gaatttaacc
    21421 aatatttcta agtttgttca caattggcgc aattcctcac attttgtttg agaaattctt
    21481 aagaaaaacg caagaaaaag agcgagacta tccgtctcac cctcttcatg gagtttcctt
    21541 actcatttgg gattgcagtt ttttctaatt ctgtttttat tgcctcatta gcttttaaag
    21601 ctcgttgtgc aattgcttca tatcttgctt ttttgtctcg aatgcgctct ggcagttcag
    21661 gaaacagacc atagttggca ttcatcggtt ggaaatgttt tccttccgca tgagtaatgt
    21721 agtaagccat acttccaata gctgtttctt gtggaaactc gactggttcc atttcttttg
    21781 ctaagcgtgc ggcgttgatt ccagcaagta gtccgctcgc tgcactttca acatagcctt
    21841 ctacaccagt catctgacct gcaaagaaca gatcttcgcg tttcgttgat tggtaagtcg
    21901 gtttcagcaa ttctggcgaa ttcataaagc tgtttcggtg catcacacca tagcgaacga
    21961 attcagcatt ttctaaccca ggaatcatcc ggaaaacacg tttttgctcg ccccatttca
    22021 agtgagtctg aaagcccacg atgttgtata aagaagctgc agcattgtct tgacgcagct
    22081 ggataacagc ataaggacgt ttgccagttt taggatcttc caatccaact ggtttcatag
    22141 gtccgaaaag catcgttttg aatcctcgct gtgccattac ttcaattggc atacatcctt
    22201 caaaatattt ttctttctca aaagatttaa gttctgccac ttcggcattg attagtgcct
    22261 catggaaagc tttgaattcc tcttctgtca tcggacagtt caagtaagct gcttctcctt
    22321 tgtcatagcg ggacttcaaa tatactttat ccatatcgat cgtagctttg tctacgattg
    22381 gtgcagctgc atcatagaaa taaaaaccat ctgaaccatt gaatgctttg atgctttctg
    22441 ctaatggatg agaagtcaaa ggtcctgttg ctactacggt gattccttcc ggtatttcag
    22501 taatttcttc gtttatcact gttactaatg gatgctcttt gacttttcgg gtaattgttt
    22561 cagaaaaaga atcgcggtca acagctaagg caccaccagc tggcactgct gtttcatctg
    22621 ccgaagtgat tactaccgaa ttcaatcggc gcatttcttc tttcaataac cctactgcat
    22681 tagccaaact gtttcctctt aatgaattgg aacatactag ttcagcaaaa ttacttgtgt
    22741 gatgcgcctc agtcgatttg actggacgca tttcatataa acggacaggg actcctgctt
    22801 gtgcgatctg ccaagcagct tcacttccgg caagccctgc tccaacaaca tttaccattt
    22861 tcaatgaaag aacctctttt ctattatttt tggatgtctt cttcgtaatc tccatttaca
    22921 cagactactt gtttaccgcc tttgactttc ttctcgacca gatatccgtc acattttgga
    22981 caatttctgc ccactggttt atcccaagaa gtgaattcac attctgggaa acgggaacaa
    23041 ccatagaata gtctgttttt cttagatttt cgttcaatga cttgtccttc atgacatact
    23101 gggcaagtca caccgatttc ttttacgatt ggtttcgtat tccgacaatc aggaaaatta
    23161 ctgcaagcat aaaacttccc atatttccct aattttatca ccatcggatg tccacagaca
    23221 tcacaatcga atcctgctgg ttcatctttg atttgaattt tttcgatttt ttcttccgca
    23281 ttcgtcagtt ctttttcaaa cggtttatag aaacgatcaa ccactttgac ccacgcttcc
    23341 gtgccttctt caattttatc caaatctcct tccatttctg ctgtgaaatg gatatcgacg
    23401 atttgaggga agaattcaca gattaatgaa ttgacaattt ctcctagttc agtcggctca
    23461 aatctttttt gtgtcaattt tacatagtag cgtctttgga tcgtatcaag tgttggtgca
    23521 taagtggaag gtcgccctac cccgttttct tctagtgcac gaatcaacgt agcctcacta
    23581 aaacgtgctg gtggttgagt aaaatgttgt tttggttcaa tatcaacaga ttggactaca
    23641 tcgccttctt caagttctgg aagaatattt tctttctctt ctttgccgtc atctctacct
    23701 tccacataca cttgcatgaa tcctttgaat ttgatttttg aaccgttcgc aatgaagatg
    23761 acaccatttt gttctaatgt cactttcatt gtatctaaaa tagctggtgt catctgacta
    23821 gccacaaaac gtgaccagat caatgtataa agtttcaatt gatctttatc taaatattgt
    23881 ttgagttcat ctggtgtacg aagcacactc gttggacgca cagcttcatg ggcatcttgt
    23941 gcgccttgag catttttgat ctttcggtga tcatgtctag aaaattcttt tccatacgta
    24001 tcttcaataa atgctgcggc ctctgctttt gctgtatcgg caatccgggt agaatcagta
    24061 cgcatgtatg taatcaagcc gactgttcct tgtttaccca aagtgattcc ttcatataat
    24121 tgctgtgcta ccatcatggt ttttcttgtt cgaaagttta acttacgggc agcttcttgt
    24181 tgcaggctac ttgtagtgaa aggattcgca gggtttcttt tgcgctcttt cttttctact
    24241 tttttaactt cgaagtctgt tccatcaatt ctagcagtga cttcttttac actttgtgca
    24301 tctggtaatt tctttttctt accgtcgatt ccccagaaat ttgctttgaa tttcttgcga
    24361 gcttttttaa aattaccgtc gatgctccaa tattcttctg gaacaaattc tcggatctct
    24421 ttttcacggt caataataat ttttaaagcg acagattgga cacgtcctgc acttaaccct
    24481 ttcttgacct tacgccagag aataggactg atcgaataac caacgattct gtctaaggta
    24541 cgacgagctt gctgtgcatc tactaaatct acatcgatcg ttcttggttc cttaaaagct
    24601 gctttgactg cgtcttttgt gatttcattg aagaccaccc gatttttttc tttcagatcc
    24661 aacccaagta ggtacgctaa atgccaagca atcgcttctc cttctctatc cgggtcactt
    24721 gccaagtaaa ctttttgagc tttttttgct gcggctttca ggcttttgat gacatcgcct
    24781 tttccgcgta tagaaatata atggggttca taattgtttt caaaatcgat acccatttta
    24841 cttttcggta aatctcttat atggccaaca ctggccatta ctttatagtt tcgtccaaga
    24901 tatttctcaa ttgtcttggc ttttgcaggt gattctacaa tcactaaata tttatacgcc
    24961 attactgaat ggactccttt ccattgtcgt gttcctacta ttaaaagagg attcatacaa
    25021 atcgtagcta atcatatcca ttcagaaaac gtttgtcaag aaaagcaaat gaaaagtatg
    25081 gtgttattct ttaaattctg ccaagatatc ttcacccgaa accgtgcaaa ctgcaccgtc
    25141 ttggatcaaa tgatgacatc cggtagaatg ttctgttaaa atatttcctg gaacagaaaa
    25201 aactgttctt ccaagatcca atgcttgctg tgctgttatc agagagccgc tttttttctt
    25261 ggcttcaaag acacatgttc ctttagacaa agctgcgata atccgatttc tcattggaaa
    25321 acaatgcttt ttcgggccgg attgaggagg atattcactc aaaagaagat gatcatttgc
    25381 aatcacctct tgcaagtcac gattttcagc aggatagact tgctctatcc ctgttccaat
    25441 gactgcgatt gtccgtccac catgagagat tgctgcttgg tgaccgtatg tatctatccc
    25501 tttcgcgcag ccactgacga tcacatatcc agcttctatc actggtttta gtaaataata
    25561 aactgttttt ttggctaaag cagaagtgtg tcttccacca acaacgctta acatgttaca
    25621 agaaagtaaa gatagatcac cattataaaa cagtacaatt ggcggatagg tcaagtgaag
    25681 cagctgaggg ggatatttgg gatcaaagca agtgataaac gactgtttcg attggatttt
    25741 gccccagtta gcgtgaattt cctgccaact tttgcgaaac agctcccggt gtttggttat
    25801 tttacctatc ttaatgatgt cttcaaatcc gatttcttta taaggatact gcatcatcca
    25861 gttagcaagt gcccattttc cttgactact gattcctttt gtataagcaa gtttcaataa
    25921 attttcttct atttgataca tcataaaacc gcctttcgtc tctctttcta ataagatacg
    25981 taaaagcggc tgttttttat tgtaaatatt tttttacagg tgcaaaactt ttacgatgga
    26041 ttggcgtaat tccttgtcgt tcaagtcctt ctaagtgttc ctttgttcca taacctgcat
    26101 tatgcgagaa accatatcca ggataaagca cgtcatattc tttcatcaat tggtctcgat
    26161 atactttagc aatgatactt gcagcagcaa tcgacacaga tcgtacatcc cctttgatta
    26221 ttttttcctg ctgaataggc aaatccaagg tcatcgcatc gatcaacaaa tagcttggta
    26281 caatggacaa tttttctact gcttggatca tcgctaattt acttgcttca taaatattca
    26341 catcatcgat tgtctgctga tcaacgatcc cgatcccgat tgccagggct ttttcttgaa
    26401 cttgttgata aagaagttct cgctttttct ctgataattg tttggagtca ttcagcccta
    26461 atatgatctc atttttcggc aagataactg cagctgccac gacaggtcca gctaatggtc
    26521 ctcttcctac ttcatcaatg ccggcaatcc attggtgacc ttgtgcgtac ccttgctttt
    26581 caaagcaaaa catttcttca tatttttcct taattaatgc ttcctttttc aatcgtcgtt
    26641 caaaagaagc cagtgcttgc tgcacgcctt tgcgtggatc atttcttaat gattctagtg
    26701 taggatcatc tattctctgt atgcttttca atcgttcttt gatactttgg atggattcag
    26761 tcatcacttt tttgctccat ttcttcgcaa cgatccagtg tgtatcgtcc aagttttcct
    26821 tgacgaatat catgcacgac taattcgctg ccacgatcat agtcttcttt atacccacgt
    26881 tttgctgtaa tatccatcag taattccggt gccaattttt cttcttgctc gattgataaa
    26941 ccataacgat cgctgattct tcctgaataa tatcgggcaa aaaagtctaa tccgtaaatc
    27001 gctaaatcat ctaaatggac aagctggtct ttgatcgcgc ctgtcagcgc tagttttttg
    27061 ccgatttctt catcttcaaa tttgggccat aaaatccctg gtgtgtctaa caattccaac
    27121 tcattaccta aacgaagcca ctgctgacct tttgttacgc caggcttatt tcctgtctgt
    27181 gcaattttct tgcctactag tcgattcatc aacgtagatt tcccaacatt tggaatacct
    27241 aagaccattg cccgaatagc gcgaggcttg atcccccgag cagcatctct tgcccgtttt
    27301 tctttcaatg cttctttagc cgcaggtact agacctttaa ttcctttact ttcttgtgcc
    27361 gtgatagcta atgcaagatg gccttgttta cgaaaatatt cttgccacag ctttgtctgt
    27421 tgtggatctg ccagatccgc tttattcaat aaaacaaggc gaggtttttg ctgaatgatc
    27481 tgatctagca ttggattacg agaagataaa ggtaatcgtg cgtctaccaa ttcaaatacg
    27541 atgtccacat atttcatttt ttcagaaact tctcgtcggg cttttgccat atgtcccgga
    27601 aaccattgga tggtcattgt tctctccatt cctttcatac cggaatattc cccggcttta
    27661 cttgtttttt tctttttcaa tataaattca cttttttatt ataaagaaat tccatctatt
    27721 agttaagtga aagtgaacca gtcctcattc tatcacatct tcactttttt gatgatgcaa
    27781 atttttattt tggtttctat atatgaatct aaatttttga cagcgtttcc taaataagag
    27841 atgatagacg aacaaaacag aaaggagacg aaacaaatga atagtatggt ttttgttaat
    27901 tttcctgttg ctgatgtcaa tcgttccgtt gctttttatg aaaagctggg ttttaaaaaa
    27961 aatgaagaat tttctactga agaagctggt tcaatggtat gggatgacac tttttggata
    28021 atgcttctct ctcatgattt ttataaaaaa tttataaaag ataaagaaat agctgataat
    28081 aaaaaaacaa gtggtgcttt gatttcgttt agtgttgaaa atgcggatga agtaaaaaaa
    28141 tttgctcaaa ttgcaaaaga aaatggtggt actttccacg aagtagatat gggaatgcct
    28201 gaagaagaaa tgttttcatt agaagtccaa gatctagacg gaaatacatt agaacccgta
    28261 tggatgaaga tgtaagcaga taactaatct gacgtttttt ataacaaaaa aacaaaggcg
    28321 gatgatacga taagtattct ccgcctctat tttttttatt cgtacaatct cttttttaca
    28381 gtttataaaa gcagtcttct cacccttttt ttaattgtta gatgccagtg tatgtaaatc
    28441 accaatgtgt catgacacat tggtgatttt atggtatcct tttattatta actgacaaag
    28501 gagagaatat tttgaacact ttgattgcaa aagacgtttt tagattgccc gaaatgaatg
    28561 caagattgtt gtcgacctct gctcttttat tagcccttga tgtcatctta tacaagatag
    28621 cgattggtcc tgcttatttt caaatcacat tcggattttt aagtatggcg cttatgggtt
    28681 atctatatgg accgatctgg gctggtttgc ttgaaatgtt gtcgaacact ttaaatttca
    28741 cgatatttgg ttcaggaacg tttcaagctg cttttcttat cacggctttc cttggtggat
    28801 ttattaatgg cttattttta tatcataaaa agataaacct gccttccgtc atcgctgccc
    28861 aattattgat tatgatccca gtcagccttt ttatcaattc ttggctgatg tctgttttgt
    28921 ttggtgcaga tttcaaagta atttttctag gtagattcat caggaatatg atccaaatcc
    28981 ctattcaaat aatggttgtt tattcattta tcaaaacttt agaggctaga aaagtgttta
    29041 aacgagctgt ttgattttac gtactaaggt agtaaactga ttttggatta aacaataaag
    29101 cgtgcagtct caacagactg cacgctttat tgtttctcac tggatccatt ttatttcatc
    29161 aagtggataa tagacaaatc ttgctttacc tagaatatca gttttattga tcgtgccaaa
    29221 cgaacgacta tcttttgaaa ctcttcgatt atctccaagt acaaaataat tatcttccgg
    29281 aagcttggat tgtcccgtta gctcttgtag tgtaaagtcc gtcgtatagg atgcatttgc
    29341 atgatctgat ttcaggtttt tagttagata aggttcctta actactttcc cattgatctt
    29401 caattgatca ttttggtagc tgacattttc tcctggaaga ccgattactc gtttgatata
    29461 aatcgtgcca tctgctaatt gaaaaacaac aatatcaaat cgtctgatct ccgaaaattt
    29521 ttccatcaca accatatcac cttgcttcaa cacgttttcc attgaattgc cttccactgg
    29581 taccgggatg aataaaaatc cccgcaacat gaacgcaagg aatacagaaa caagtaagta
    29641 tttgaataca agccagaagc gattaatata gcgttgtttt tttgtcaatc tctatcaatc
    29701 catccctctt tttttcattc tttctttatt ataagaaaaa tatcaaaaaa atgcgactag
    29761 atagctgaaa taaaaataat tctttcaatt aggctattgg acaaaaaact tttttgatca
    29821 taaagtacaa aactatctct tatatgtcat gattattttc taaatatttt tcccatgctt
    29881 cgtcaaagat atccatggac ggcaaatagt cgacattcag ttccagataa ttagaaattt
    29941 cttcgtagct agagctgtgc ttaggaaatt ggatgtcttt accggcttct gtagcgaaaa
    30001 cagataaagc atcatgtgtg ccaccgcctc gcagtgtcat taaataatga aaaaagcttc
    30061 gattcaccct tcgttcctcc aattttcttc gataaaggca gcccggcgtg catggtttct
    30121 tttaaaattt tcaggatgct ttttataata ctcttgatgg tcttcttctg ctagataaaa
    30181 tggttgggct ggttcgattt ttgtaacgat tggctgttcg aaacgtccac tttcagctaa
    30241 ctgtttctta cttttttcag caatgatccg ctgttcttct gtcaaataaa agatcaccgg
    30301 gcgataattg tcaccgcgat cctcgaattg tccgaaagcg tcagttggat cagtttgctg
    30361 ccagtaaagt tctactaatt gttcatatga aatcaatgtt ggatcataga taatctccac
    30421 tgcttctgta tgtccagtcg tttggctttt tacttgttca taagtcggat ctggtacatg
    30481 ccctccagta tatcctgaca tcactgtatg gataccagga agcgtgtcga aaggctggat
    30541 catacaccag aagcagcctc ctgcaaatat tgctcgttcc attatgattc cccctctaat
    30601 actactcatc cgatgatttt ttattttctt ttgtcttgct ttcagtactg ctggtgctag
    30661 ttttgttggt atttgagttg tttgaagcat cagaggtatc ttttggcaaa tatagactaa
    30721 accgaatgtc atcatttact aagtcgattt tatctgcttt aatatacatc ccgttttgca
    30781 ttcggaattg atccaaccta agcatgatag tcagatcatc tgtattgact tcgatccaat
    30841 ctggtaattt atagtttctc ttgatcatat tcatcacatc tttgattggc aaactcaatg
    30901 ttccgataga aagacttcta gcttttagtt ggacatttcc gttttccatc acatacggat
    30961 caaaatataa atagaaatcg ataggaaaac ctagtacttc gaaagtacca ttgagtagtg
    31021 cttcattttc caaataaaat ttatattcaa tatcactatc tttttgatac tcactaagga
    31081 aaaagctgat gatttgattg actttttctt tatttgaatt gatcgttaag acaggatctc
    31141 cttgtctttc cgtaagagct ggtatttctg ggagttctgg ttcccgatta gcaaaaatac
    31201 ggctgcctaa aaatacggtc gaaccaagta ctaccccaag aaggataagg aaggcgaatt
    31261 tccagtaatt tgttttcttt gttttgtttg aagatacgtc agatcttgtt ttcatgttta
    31321 ttgatcatcc tccttctcta accattggtt ttgtgtctcg atcagctgat ctctcactgc
    31381 atttgccatc aattgataac ctaaattatt tggatggaaa tgatcttctt catagagcac
    31441 attatttttg atgccattta gattttctgt cgtgctagtt gtttgatctg ttccttcttc
    31501 tgtgacacct acttcctgat tcaatccttg ataaagcaaa tcattgattg ggataaagta
    31561 gcagttcggc attttttttg tcaccgtttc tgttcctttg ttccaattgt taacaattgt
    31621 ctgcatgtca gtgatctcag gaaagttcaa ataaaatgga ttgtagatgc ctaaaatgta
    31681 aataggtgcg gttttattga gggatcgaaa ttcttccaat agttctgtga cattttcctg
    31741 atagtttttc agtggctttt taaaggaatt gaccgttaac ccgaataaat tattttggat
    31801 aactttcatc aaatcattac cgccaacagt cagtgtgata aaatcagctg ttttgatatt
    31861 gtttcgaatt tcttcttttg tttttactcg ctttaagatc tggtcacttc gttcaccatt
    31921 tacaccatag ttttctgttt caaccgcggt caaattataa cggtctttta gatcatttgc
    31981 gacaagggga acgaatcctc cttgcttggt ttgatctcct actccttcag tcagcgaatc
    32041 tccgatagct atgtagtgaa gtttttcttt tttatttgga tcttctctca cagtagttac
    32101 tggtttcatt agtgggccag ctttcggtat agaagcagct aaacctgcat agatgatgat
    32161 cgcaattaat aatgggaaca aaacgaggat caaatgtcgt ttcgttaatt tcattttttc
    32221 acctcttgta ttcgattggt tctaatgtta tcaaaaattt tatcaaaaag gtagtcttta
    32281 tgaaaaaaga aaacaattat tttcctaatt ttataaagaa aaagagaata agctgcaagc
    32341 ctttcaggct gagggcttat cctcttttcg actaatctac ataatacatg attgcaaacg
    32401 cgtctttacc agtatgcgta gcgataaccg gattcgtatg cagtacaggg atatgcatgt
    32461 ccgggaataa ttcttgcaaa ccctctttga attcttgcgc caattctttt gcatccgcat
    32521 gtgaaatacc gatttgtcgc acatttggca attctttcag ttcatttttc aaattattaa
    32581 accatttatc gaacgttttc tttcctcgcc ccttagttac agggattaat tcagaatggg
    32641 cgaaattcat caccacgcgt atgttcaaaa cactagacag caaaccagtt gttcggctga
    32701 ttcttccacc ttttaccaag ttatctagtg tggatattcc gatgtataat tttgtattgt
    32761 cttgtacttt tttgacttct tcaaggattt tctctactgt tttaccttct tttgctaatt
    32821 cagctgctcg aattacctga aaagataatc cttggtcagt ggtatcactg tcgataaccg
    32881 ttactttcgt ttttgttaga ttactggctt gtcttgctgc ttctactgtt ccactcaagc
    32941 cttttgtcat atggatcgaa atcacttcac tgccgtcttc tcctagctca tcatacatcg
    33001 agatgaattc tccaattggt ggttggcttg ttttgggcag attttttgct ttcgccatca
    33061 tttccataaa tctttcgccg tctagttggt catcatcggg atatactgtt ccgtcgatca
    33121 tgatggatag cggcatgaca cgaatgccta aagcatcgcg tacatttttt tccatcgtac
    33181 atgaagaatc tgttacgatt tttacatttg tcattatttt ctctctctct ttctttaaca
    33241 agatgcctta ttcatgttaa actacatgta agacaatttc tatttttagt ataacagaca
    33301 aataatgatt tttcattaat tacacctagt aaaggagccg ttttcatgga aaaaacgaaa
    33361 tttaatcgaa cgtatgaaat attaaatgaa gtgtttaatg cagtcaccca tggaattggg
    33421 actggattga gtatcgcggg actagtggtt cttttagtaa aaggtgccca cttgcattcc
    33481 tccctccata ttgtctctta tgctatctat ggatcgatga tgattctttt atttttaacg
    33541 tctactttgt ttcattcatt gatttttaca aaagcaaaaa aggtgttcca agtattcgat
    33601 catagttcga tttttctttt gattgcagga agttatactc ctttttgttt gctgagtatc
    33661 cgaggatggc taggttggac tttatttgta ttaatctggt tgtttgcgat tcttggcatt
    33721 atatataaag cgttgacgtt gcataaacgg gagagagtat caaaagtatc tacgatcatc
    33781 tatattttta tggggtggct ctgtgtcgtg gctgctgttc ctctttatca ctctcttgga
    33841 atgactggtg ttattttgat ggtagcaggc gggctttctt acacgatagg cgcattcttt
    33901 tatagcttga attctatccg ctatatgcat gttgtttggc atttgtttgt tatgttaggt
    33961 gcaggtttta tgtacttctc tgttttattc tttacttaat aaaaaaaata aagctatgga
    34021 tgggacagct ctaaataaca gctagtctat ccatagcttt tttatatcgt caattcgcct
    34081 gtcccgcttt ttacaggatt ctcattacgc tgatacgttt caaattgatg cggataacga
    34141 tttttttcat ccatttctcc ttgactagta ttgatcagtg accaatcaga ccagtcaaca
    34201 ggtggaaaat aagcatctcc atcgaataca tgatggatga ctgtacggta taatatcgta
    34261 caaaatggca gaaattcttg ataaacagct gaccctccag cgatgaaaaa gataccgtct
    34321 gtttcttttt ccagagcaag cacgtcttcc acactatgca tgacatgcac gccttctgct
    34381 ttataggaag aatctctcgt caaaacaatc gtctgacgat tcggcaacgg acggctaccc
    34441 atcccttcga atgtttttcg tcccatcact agtgtatttg cttctgtcat ttgtttgaag
    34501 aatttcagat catttggcaa acgccaagga agacggtctt cttttccaat caatccgttt
    34561 tcatcctgtg cccaaattgc agctaacatt ttctcatcct ttctgattct aaacagcgat
    34621 aggagctttg atcgccggat gtgggtcata tccttctaat tggatatctt ccatctcaaa
    34681 atcaaatact gactgctttt cctgattcaa aactaaagtc ggaaatgcgc gtatttctcg
    34741 gctcaattgt tctttcattt gttcgatatg attgatataa agatgagcat ctcctaatgt
    34801 gtgaacaaat tcgccaaccg ctaaacctgt ttcatgtgca attaggtgag tcagcaacgc
    34861 ataacttgca atgttgaacg gaacgcctag aaacacatcc ccactgcgtt gatacaattg
    34921 gcagcttagc cgtccctctt gaacataaaa ttgaaacatc gtatgacaag gaggcaaagc
    34981 catgctagga acatcttccg gattccatgc agaaacgatc aatcttctag aatcaggagt
    35041 ctttttgatt gcttcgatca cttgtttgat ttgatcgata aagccaccgt cttttgtttc
    35101 ccagtgacgc cattgatagc cgtagatgtg tccaagatcg ccgtatgttt cagcaaagga
    35161 atcatcggtt aggatcttct cgcaaaaaag cttgctctgc tttttatatt cttcattgaa
    35221 ttcaggatct gtcaaacatc tatggccaaa atcagtcata tctggtccat tatagtctgg
    35281 acttttgatg tagcgttcaa atgcccattc atcccaaata tggtttttat tttgtaatag
    35341 atagcggata ttcgtatctc cttttaaaaa ccaaagcaat tcgcttttga tcaatccaaa
    35401 aggtactcgt ttcgttgtta acaaaggaaa ccctttttgc agatcaaacc gcatttggta
    35461 tccgaataag cttaatgtgc ctgtgcctgt tctgtcttct ttacgatgcc cttcttccaa
    35521 caattttctt cctaagtcaa gatacgcttg ttccatcctt tttcctccta atcttctacc
    35581 cattcactca agtattccca ccgtgccatt ttttcttcaa gttgtgcttc tgtttcagat
    35641 acgtcgtttt gcaattcttg taatcgagtg aaattatctc cttgatgatt catctcttct
    35701 tggagcaatg agattttttc ttcaagttct gcaatctcat cttcaatcgt ttcccattct
    35761 ttttgttcca tgtaagataa tttcttcttt tcagcaggtt ccttgctctg tatttttggt
    35821 ttcgcttttt cagcaggctg ctgggtctct ttttgcttca gtaaataact ggacatgttt
    35881 ccgtaataaa gttcttgttt tccgcttcct tggaatacta ataacttatc tgctaccttg
    35941 tccaagaaat aacggtcatg ggaaacagtg atcacagcac ctggaaaact tctaaaataa
    36001 tcttccaaaa tcgtcaatgt tgctatgtcc aaatcattgg tcggttcatc caataataaa
    36061 acattaggtt gctgaatcag cagtttcaac aaaaataacc ggcgtttttc tccgcctgaa
    36121 agtttgccta tcagtgtccc atgcataaat cttggaaata aaaatcgttc aagtaattct
    36181 gctactccga tttgactgcc atctgctgtt tttgcttctt cagcagcttc ttgtaagtaa
    36241 gcaatcatcc gtttatctgg tgccatttct tcattttctt gtgtataata cgcaagatga
    36301 accgtttcac cgatagacat tgtaccgcta tccaggggaa ttctgccagc tagaatatta
    36361 agcaatgtcg attttcccgc accattttta ccagtaatac ctagtctttc ccgagattga
    36421 atcaataaat ctaaatgttc tagcagtgtt tggtcattta tacggtaact gccatctttg
    36481 atttccagta cttttttccc taaacgttgt gtcgctaggt tcagctctaa atcatcttct
    36541 tggtttactt ggtacaagtt ctcctttaga tcttcgaaac ggttgatccg tgcttgctgc
    36601 ttggtgccac gtgcctgtac gccagccctc atccaagcta attcctgttt ataaagccgc
    36661 ttgcgctttt cttcttgttc tacttctaca cgatcacgtt ctgctttttc taagacgtaa
    36721 gcttcataat ttcctttata ctcgtataat tttccaaatg aaagttcgaa gattcggttt
    36781 gctactctat ctagaaaata acgatcgtgg gtaaccatca atagtgcgcc acggtattgt
    36841 ttcaaatagt tttccaacca ctcgatcgct tcataatcta aatggtttgt tggttcgtct
    36901 agtaatagta aatcgggttc atcaatcaac acttgcgcca gactaactcg ttttttctga
    36961 ccacctgata aagtactgat ttttttatct aattcactga ttcctaattt ttgcaaaata
    37021 atctttgcgt tggtatctgt agtccaagca tcttctttat tcatttgttc ttctgcttta
    37081 gcataacgtt tttgcacatc agggtcttct ccgttttccc ctagttcgat caatgcttcc
    37141 tcatatgctc gaacagtttg gatcaagggg ctgtttcctt gaaagactgc ttggagaaca
    37201 gttaattcat cggaaaattc agctgcttga gaaagataac caatacggta atcagttgga
    37261 taaaacactg tttgccgatc accgtcgctg ctatcgatac cggcaataat atttaagaga
    37321 cttgttttac ccgtcccgtt cgtcccaatc aacccgatac ggtctttttc gtggaccaaa
    37381 aaagaaatct ggtcaaacaa atctttttca ccataagttt ttttgaggtc agttactttt
    37441 aattctttca acatgttccc tccttatctt ggatattgta tcataaaaat agtgaacttc
    37501 aggaaaattt ttttggtatt tgttcttttt gtgttctcaa aaaaagaaca ggaccgattc
    37561 gatcctgttc tgatatgttt tgttttttat aaaacttgaa aatttttacc tttttatttt
    37621 attggactcg ttttttttga ctttgctcga aacgcaaatc ggaaagaaaa gttatcagat
    37681 ggtttgttca tcgtataaga ttctaacaaa ttgatattcc tgcgatagct gttcgcagaa
    37741 aacgtcgtga ttttttcagc aacttcgtat tcatcaatga tttttctttc aagataataa
    37801 cctcttagca tttttttcgt aaacagacta tcttgctgga tcatccacga agaaatcaaa
    37861 ttccccgagt tcatttttat tccttcaagt ttgcgttgct ggataaaaaa ttgaaccatc
    37921 gtttcatcat aaatatcagt caagttatct aaactttcaa taatcaactg agtatttttt
    37981 tgatatgtct ctctgatttc ttggatatct tcatcactaa taaatgtctg acgatcagca
    38041 tatcttcttt gccaaaacat tttcggatgg agaacgattc gcattaacct acggacaaca
    38101 atgaaccaaa agccgataaa agacaaaatc tggcttgtga tagaatgttc gaatcgagaa
    38161 agaaaacggg agtaaaaacg ataaccatta gaagaaagct tgcccgaacg ataactttca
    38221 tccaatccat cccgttcgat cgacaaaatc agtgcttgta tttcttgtac ttcttgcttt
    38281 tcatcatcgg ttaaatcttc caagtacaga tcacgtatcc gttcttgata attttctatc
    38341 actgcttcag tggctaaaaa ttcttttgta ttacgatcaa tttcctttat ttcttttcgc
    38401 aaaacttcaa cgacctcttc taaaatcatc aaagcattca aatccgtgct tttcgcttcc
    38461 tctgtttccg tcagcatagg caagacaatg atccctatga ccaaagtcac taagatgaca
    38521 cccgccgtca gaaacaaaag caaagaccgc tggtaaaaca tcatattatt gattgaaaat
    38581 ggcaaaataa aaatcgtagc caaactgact gttcccttta caccgccaaa tgttaagatc
    38641 aagatctcgt tcaactgttt tttgaacttt ttactgccat ctttgaatac ataaaaaaga
    38701 ctgatagaaa tgaatctgat cacaaaaagc atgacagaga ttaatacgat aatcgctaat
    38761 aaaagaccgt ttggatataa accgtctccc catactggcg agaacacttg cgtcaattcg
    38821 attcctaaaa atataaatac taatgcattc agtgtaaaag caatagttgt ccatgtactg
    38881 tgggacaagc tcgacaattc tgcatcaaac acagagattt tcctaaaacc agaagcttgg
    38941 agaatacctg ctgccactgc agcaatgatt ccagatacat caaatgcctc tgccaataca
    39001 taggctagaa aaggaagcaa aagttcgatc agcagatacc ctgtgacatc ttgtgctgat
    39061 gctttttcga tcaaatgaat tattttttgt ttgaaccaga caagcaaaaa gccgatcaaa
    39121 gctcccccaa tactagaaac tactagactc atccccgcgt ttactgctga aaaagatccg
    39181 gtgatcagcg caccaagagc aaactggaag gcagtaacac cagatgcatc attcaaaaga
    39241 ccttctcctt ccaaaatatg catcgccttt ttagggatat tcactcgtcc agaaagagaa
    39301 ctgactgcta ccgcatctgt cggacctaac gctgctccaa aagcaaagca ggcagcaagt
    39361 gggacacttg gcaacaggaa tgataacgtc gcacctaccc cgacaagggt caaaatgact
    39421 cctccaaatg ccaagaacaa aattgtcccg aaatttttga gaatcgaaga aatatctgct
    39481 ttttctcctt ctctaaataa caatggtgca ataatcataa ctaagaacaa ttcaggttca
    39541 aaatcgattg attgtcccca ctctgtcaga cctaatacca ctccaaggaa aatttggatc
    39601 agtggagctg gaatcattgg caaaatgcgg ctagctatgt tcgataaggt gatcgttact
    39661 gcaaatacaa taactaactc agcaaattcc atcgtccatc ccctttattt tttattttgt
    39721 gtaagcattt ttaaaaattg ttcttttttc tttaaaattt tggtttcgac tacttcaacc
    39781 tgtgtttggt tcccctctaa ttcacactgt acacgttttg actccaaacg aagcagatca
    39841 tgtttcagac gttccaactg acgatacatc aatatatttc ttttacgcaa cttacaaagt
    39901 atttgacggc ggtcatccat cgaaagggcg ccatagcgat catgcaacag ttttttttgt
    39961 tgttcaaaac tctctctcat tttccagatc tcctatacac aaacttccat cattatagca
    40021 aagttctttt tggatgaaaa atatttcatt gtttcatcgt ttattgctag ttagaaattt
    40081 ccataaattt ttcaacgcct cattctattc caaatagtat cagatatcaa gtaaaactta
    40141 agaaagataa aagtttctga caaaaggatg tttactttcc cattcctgcc tttcttgttc
    40201 ttttatcaca acaaaaaaat ggggctgcga caaaaatctt gtcacagtcc cttattctga
    40261 ataaacggtg tggaagagac agcctatcag tagtaataag acacatctct ttcacagcct
    40321 catttttgcg taaaaatcga tttattcgaa aaattccccg tatacatctt ctacacgttg
    40381 atctggattg atcacgatat acaagttacc tgttttttcg ctgatttttg ttgattgata
    40441 ttgattgtct aatccttctg ttcctttatc tagatatggg aagtacacaa gatcaggtgt
    40501 gccatttcgg taaaggattt ccattcgctc gatatcttcg tatttcaatg cgcgattaaa
    40561 catgcccatt tccagtccgc ctaggttgat atcatgtgtc gcaactgcgt ccccttcatg
    40621 gaagatttcg attttaaatc cagcacacgg atggatttca acaaattcac tgccattgat
    40681 tcgtccaaaa cttgttgtga tttgtttgat ccaaagatcg ccgatatgac ggcggttgat
    40741 cgtccaggtt tctccatttc taaaataaaa ttttagcgct gtcatttctt tactcattaa
    40801 aaagactcct ttatgatgta atttattctt gtactacctc agtttagcac aaatttatgt
    40861 aagtggtcac atcttatgtt ttttcttcta atgacgtatc tgcccacttt aagtagccaa
    40921 caaagctgag cagatacagt ctagtgtttt ttaaatcaat ttttctttcg ataaaaaatc
    40981 caaaacctgt tttttatcat tttttattct ttgttctacg acggcttttt cagctaattg
    41041 caaagcttca ctaacccact taccaggttt ttttccagtt tcttctagca agattttacc
    41101 atctactgct aattcgctta atgacttgat aggtaaacga tcataggatt ctcctacggc
    41161 atccatctga ggtgtttcgt caaaatagat catcaattct tctacttgca gtgcatgttt
    41221 tttgcctaag aagtacaatt cgaatggttc caaagctcca gtttctcgtt gtcgtagtcc
    41281 ttggacaagt gcttgaacgt ctttaatcat ttggttagaa cacttccacg atttcaaaaa
    41341 ggaacgaatc tcattttctt ttagatccag ttggctgatc aataatgccc atgcttggct
    41401 ctcttctttc aatgggacgg atggtaattc agcaaaacgt aaaagtgctt cgccttcttc
    41461 tttcaaaccg ggacagcaaa tgtagcactc tgtttcaata aatgcatgga ttcccgcggc
    41521 ccgttcattt cccaagagca tcttgatgaa ttccacattt atacgttcta cagatatttt
    41581 acctaatagc tcgtggtttt ctttgatggc gtagaaggtt tcttcctcta atttgaaccc
    41641 tagttgactg acaaaacgca acccgcgcat cattcgtaag gcatcttcat ggaaccgctc
    41701 atgaggattg cctacagctc gcaagatctg ttcttctaga tcttgcagac catcgaacaa
    41761 atcgatgatt tctccatctt ctttcaaagc aaacgcattg atcgtaaaat ctcttctttt
    41821 caaatcttct gccaacgaac gaacgaattc gacatgatct ggtctacggt agtcctgata
    41881 agttgattcc gtccgaaatg tggtgatctc atattgttca tcattttcta ataccaacac
    41941 agttccatgt tcgatcccaa tatctatcgt ttttggaaaa agctgtttga tttcttctgg
    42001 aaatgcgctt gttgcgatat ccacatcatg gattggctgg ttcagcaaga tatcacgaac
    42061 acttccacct acaaaataag cttcatatcc tgcattctct aattttttca gaactggaat
    42121 tgccttttgg tattccagag gtaattgttt taatttcatt tttccaacct ttcctgcgtg
    42181 tttcttgtcc tgagataaag actcagttgc atggtgactg tttgacaagt ccgtttcaga
    42241 gtcttcaagg ccaggaccac ttcatcttgt ttactcgtcc ttacgctcaa agcgattatg
    42301 gtctctttga ttgaattttt ccatgttttt ttcaaaaact tcagttaagt cgatattcaa
    42361 tgaattagcc atgatcatcg taacgaataa aacatcacca agttcttctg ctaccgagtt
    42421 aactggttca gaagcctttt tgcttttttc tccataagtg tggttgactt ctcttgccaa
    42481 ttcacctact tcttcagtta atcgagccat ctgagctaat ggagagaaat accccacctt
    42541 gaattgctgg atatattcat ccacttcctg ctgcatactg tataaagaac gctttttatc
    42601 catctttttc cctccaaaac tatcttacca aaactttaac gcaaatccta gataagagca
    42661 attgagaaaa tcgaatttcc atgctacatt taggaaggaa tttctaaatg gaaattctgt
    42721 gaaaatacat aattgaaagc tggaattcat atggtcaaac gaaaaacagt aaaagaagtt
    42781 ctttttatca ttataggaac aagtatttat gcatttggtc ttgtttactt gaacatcgct
    42841 aatcatttag cagaaggggg agtctcaggg atcaccttga tcttaagagc tttatttggt
    42901 attgatccag cttatacgac tttattaatc aacatcccat tgattttact aggaggaaag
    42961 atcctcggta aacgttcctt ggcctatact gttctaggta cactttcttt gtcattcttt
    43021 ttatggatct ggcaaagggt tccactagaa atcaatttac agcatgatct actgatcgtt
    43081 tctctacttg cgggtttagt tgcaggtgta ggaagcggga tcgtctaccg catgggtgga
    43141 acaacaggcg gcagtgatat tatcgctcgg attttagaac aaaattacgg catcactatg
    43201 gggcgttccc tattggcatt tgatatcatc gtcttacttg cttcattaac ctatatcgac
    43261 ttgaaacgta tgatgtatac gttgattgtt tcgtatgttt tcagtcgtgt gattgattcg
    43321 atcttagatg gcggatattc tgcaaaaggt ctacttgtcg tctccaacaa aagcgaagag
    43381 attgcaccac tactgatgac cggtctgcaa agaggtgtga ctttcctttc tggagaaggc
    43441 gcattctctg gtaattcaaa aaaaatcatc tatatggtcg ttagctctag tgaattaact
    43501 gaagctaaga ggatcgtcca cgagattgac gaaaaagctt tcctttcaat catcaatgtg
    43561 catgaagtgg aagggcaagg ctttacttat ttgaagccac agacaagatt actgaaaaaa
    43621 ctataagaaa aaagacaaag ataggctatg aggaaagatt ccacatccag ctgacctttg
    43681 tcttttttag tactatacta tttttaacta attgttatgt tcacttaccc ggttactttt
    43741 cttttgtgtc tgcaaaagga tctttcggtt aacttcatca gataatttca aaaaagcatc
    43801 cctatctcca tcttctagtg cttgatcgat ttgagcataa agcattttta gttttgcctc
    43861 ttcttctttt tcttgaaaat atcgttcaat taattcagta tcttcttcac tgactgaaga
    43921 atcccaagaa gaaaatggat tgtcttctaa gatagataga tattctcttg tctgccaagc
    43981 attttcgaat atacattcca catataaagg ctttttccaa ttcaaacgga tttcatgaaa
    44041 aatctggtct gtgtccgtaa attcttttcc ctgcaaaaac aatttcatgg gttccccttc
    44101 aacagataga tcactgatct gcagtcctcg atccgcaaaa ttcgcccctt caacaaagtg
    44161 gacattgctt aaaatcgctt catgattggc aagataattc aagatccaaa aaacttctct
    44221 tcggctaaaa gatacatgat tgaccatcca cgttaaaaaa ttcttctttt cccgtacatc
    44281 aattagcatt tttctcacct ctctgttagg tcatctaata atgacagtgc ctccatatct
    44341 cctggttcaa gtgttaaata ataagtcaat aattcttgcg ctcttttcag ctgcccttct
    44401 tctcttaaga aaaaggcata ctctttcaag aaatctggtt catgtgatag ttcatgataa
    44461 gcctgttcat agtgtacagc tgcaacggta aagtcttcta attcattata agcatgggct
    44521 aggttccatt ctgcataagg attagcagtt tcttccatct gattgatcac ttgtatcaca
    44581 tcttcatatc tttcatcatc caagtaaaga ttacttaaag taagtaatgt ttcatcttgt
    44641 ttgtctccta cttctaaagc tttcagtaaa agttcttctg ctcgctgtct atcatgcaga
    44701 cggaaagcat tttccgaggc caggtgataa agttcgactt ggtaaggatt ttctttgatt
    44761 ccttcttcta acactgtttg tgcttcttca atcatttctt cttcttgaag agcttgtcct
    44821 agatatagat ataacgattg ataatgcgga ttaacttctc gaagttcttc cagatagttg
    44881 attgcctgct gattttcttt caattgaaga ttcgtaaaag ctagctggaa cagtcgatca
    44941 tctgtccttt cttctttcag tgcttcttct aaaggtacga tagctgcttc gaattctcct
    45001 tgcatactta aggattgtcc taaacgttcc tgcatcgaga ttcctgaaat ttcatttatc
    45061 ccgcttgcca acaatgtttc ataaaccact gctgcctctt tgaaacgatc aactgaaaaa
    45121 tacaattcag ctagcgcaaa ctggatcaat ggttcatcag gaagcaaccc agctgcttct
    45181 ttcaattttg cttcactcac ttcagggatt ccgataactt ggtacaaatc agctatcgcc
    45241 aataagcttt gcggatagta atcactttgc ttatccactt tttccaggta ttcaaaagct
    45301 aagtcgatct cattatcttc gattgcaatt tctgctaacg ggatgttcag tccgtcattt
    45361 tctgggtatt ggttgatcag ttgttcaaaa atctgttttg cttcgatcaa aaagccaata
    45421 gccagcaatt cttcgcctag ttcagctaat atatctgctg ggtcttcttt taatgctttt
    45481 tctaaaaaga tttgtgcttg tgacaagtct tcatctgata aagcttgcaa cattttttcg
    45541 ctatttgtat tcatttcaat tccctcttat ctcactgacg atttgttgaa ataccgtcat
    45601 tgttctcaat aattcttttt cgtttggatg ttggtatccg tctattatac ctattttttc
    45661 taaaacaagt atttttttgc ttttttctgc caaaaaaaga actttttcca aatactccat
    45721 ctttgaaatc tgttccagcc agtggattgg atagccaagc aagtccagcc acacaaaaaa
    45781 gttttttagt tggaaatcaa aaggatatgc ttggagattc cacacgaaat gaaacatcat
    45841 tcctaaaaat tttttcatac tagcagaaag tagatggcca ttttcggtca gataaaaagc
    45901 ttgttcaaac agtttaccat aatttgacag gttttctgcc tctaattgat aaaaatagat
    45961 catttcttcg atcatcccgg caaacggccg agtcgttaat tgctttttat ttggatagtt
    46021 tttatacaga ttttgcaaaa aacgatgatc acaaatcatt ccgcaacaga taaaagtcaa
    46081 aaagtcaatc tgctttccgt ccacctgccg ctcgctgatt gtttgatcat aaacgatgcg
    46141 ttctggcaga ttgatggttt gcagcactac acgatttggt agctgcaaaa tcgtccgctg
    46201 cggtgttaat gctcggctta gagaacggat cgatacaggg atcatccata gtgatgaatc
    46261 aagggttgta tgtttttgaa aaaaacctgc cagatccatg atcccttcat ttccaaatcc
    46321 gatgataaac atggattgat tttcgggata gcgtttgacg aaatcaagca ggttctttaa
    46381 ttctgttaaa tgattgcatt gagtgttggc acaaatatac cagtcattgt ttgcttgatt
    46441 tgcaaataac gggttgatct tatctgcaaa caaatcataa tagcgctgat tcgttaaaaa
    46501 taagattggc tgatcaatgg aaagttctct tagctgactg actagcgttt ctccgtaaaa
    46561 cactttcgtc aacgcatctt ctacttgata atcaagttcc atacgcatcc ctcctcttct
    46621 tatttttatt ttaccattag taagcaaatt ctagaaaaga taagagcaaa gctcacctca
    46681 taacttgcaa ataaataaaa attaaataaa atacatgtgt tttctatgta tgacatacgg
    46741 attcggaatc tctgtctctt atttattcct tttctgctag gaaataaaaa gaaaagcact
    46801 tgtagcccta gagccacaag cgctttttca actgtcttat ttaacagcgt cttttaatgc
    46861 tttacctggt ttgaatgcag gtactttgct agctggaatt tcgatttctt caccagtttg
    46921 tgggttacga cctttgcgag ctgcgcgttc gcgaacttca aagttaccaa aaccgattaa
    46981 ttgaactttt tcgccgttag ctaaagcatc ttgaatagtt gaaaatacag catctactgc
    47041 tgcagttgca tcttttttag ttaaatcagt agctgaagca actttttcga ttaattctgc
    47101 tttgtttgcc atggatagat tcacctcctc cacaagagat atgatagaaa tatcatttag
    47161 ttaaaacttt ctgagtggtc cagaagttta aaaagaaata ttgattaact tcaatattct
    47221 gttatcacga tatcatatga accgcttaat agcaaggttt ttaccaattt tcacatgctt
    47281 tttttagtca tagaaacgta acaaacgaaa ctgtcttttt cttttgaaaa aaaaacgtcg
    47341 atatggtaaa atgcctattt tctcctacga gggataattc ggatggacgt cccttcaaaa
    47401 gtaaatgctt ttctgatttg attttctaag aaacgttcat aagaaaaatg catcaattcc
    47461 tcttcattga caaaaacgac aaatgtaggt ggtttgacag cgacttgtgt agcataaaaa
    47521 atcttcaggc gttttccttt atctgttggt gttggattga ttgctacagc atccataatg
    47581 atatcattca acacagccga agagatacga agattctggt tcatacttac ttgttcgatc
    47641 atttcaggca gacgttccaa ccgttgttta gtcactgcag aaacaaaaac gattggagcg
    47701 taatcaagat accggaattc atcacgaatc tcttgttcga aatcacgcat tgtattggtt
    47761 tctttcttta caagatccca tttattcacg acgataataa ttccacgacc agcttcatgg
    47821 gcgtaacccg ctactcgctt gtcttgttcc cgaatccctt cttcagcatt cagtaccatc
    47881 aaaacgatat cagaacggtc aatggcacgc attgcacgca tcacgctgta tttttcagta
    47941 ttttcgtata cttttccgcg tttacgcata ccagctgtat ctatcattaa gaatttctga
    48001 ccactttcag aaacgaagtg ggtgtcgatt gcgtctcggg tcgttccttc aatatccgaa
    48061 acaattacac gttcttctcc taaaatcgca ttgatcaaag aggatttacc aacatttggg
    48121 cgaccaatca aactaaactt aatcgtgtcg tcgtcctctt cttcagtctc ttcagagaaa
    48181 tacttcactg cttcatctaa aatatcacca atccctagtc catggcttcc tgaaacagga
    48241 taaggatcgc ctagccctaa ggaataaaat tcatagatat cgtttctcat ttctggatta
    48301 tctactttat tgactgctaa aatcactggt ttattacttc tgtataaaat tttcgcaacc
    48361 atctcatctg cgtctgtcac gccttcgcgt ccgctgacta cacaaatgat tacatctgct
    48421 tcttcaatcg cgatttctgc ttgatgcttg atctgatcca taaaaggttc atcgccaaga
    48481 tcaattccgc ctgtgtcgat cacgctgaat tctcgaccta accattcacc ttttgcataa
    48541 atacgatcac gagttactcc aggagtatct tcgacaatcg agatacgctc accagcgatg
    48601 cggttaaaaa tcgtagactt accgacgttc gggcggccaa cgattgcaat tgttggattt
    48661 gccatattat ttcctcctca caagtttcca atctcactta gtttaacgaa tgcattgcca
    48721 actttcaagc atagattgtc aattaaacga agtattttta ttccttattg aaatactaat
    48781 cgcttttcaa aagaaaaact gagacaaaag cagctgcttt catctcagtc acagatacaa
    48841 aatcaattat ttttcagaat catcggttgc tttttcttct acttcttctt tcaacgcatc
    48901 acctaaaatg tctcccatag taaatccagt attttcttct ggtaattcat aagactcaac
    48961 attttttggt tcttcttctg aagctggttt ttcttctaaa gctttgatac tcaatgcaat
    49021 acgatgttct tctggatgga tctctaatac tttcactttc acttcgtcac cttcatgaag
    49081 cacttcatgt ggtgtcgcaa tatgtttatg tgaaatttga gagatatgga ctagcccttc
    49141 tactccaggg aacacttcaa caaaggcacc gaagcttgtc aaacgtttaa ctgttccgtc
    49201 taatactgtg ccaactggag cttttgtatc gatttctgac caaggtccag gctgtgtgtc
    49261 tttgattgac aaggaaacac gttcttgttc aggattgata gacgacactt tgacatttac
    49321 atcatcattg acagccaata catcacttgg ttttgctaca tgactatgtg aaatctctga
    49381 gacatggact agaccgtcaa ttccgcctag gtcgacaaat gcgccaaaat cagtaagacg
    49441 tgcaacacgt ccactgagaa catctccctc atggatagtt gaaagaattt cttttttctt
    49501 ttcttctctt tcgttttcca ctaccgcttt atgcgataag atcaaacgat tttctgatgg
    49561 ttcgatttcg atgattttaa agctcaatgt tttgccttta tagtctgaaa agtctgcaac
    49621 aaaatgatct tccaccattg atgcagggac aaaaccacgt acaccgacgt caacgactaa
    49681 accgcctttg acaacatttg taacaggtgc ttcgatgacc ttgccctctt ggaaatctct
    49741 ttcgatatct tcccaaactt ttttagcatc taaacggcgt tttgaaagta gatagctgcc
    49801 gttttcttta tctttgccga tcgtactgat aacgactaaa tctaatacat caccgacttt
    49861 tactacttcg ctgatatctt ctaccggcaa agttgataat tcttttgccg gaacgactcc
    49921 ttcaactcca gccccttcga ttccgacaat cacttgtttg tcttcaatga ctaaaacttc
    49981 gcccttaaca acatcgccaa cttttacttc ttgtacgctt ttcattgcgt cttccattga
    50041 ctggttgttc tcttgatttt gatcaaattc tgtcataaca cttgtcctcc taaccaacat
    50101 tctgccgtaa gtacggtact gtttttagtt taattgatta ttcaaaaaaa ataaagcgat
    50161 ttcgttcgtt ttccttgaaa aaacaattgt ttttcatttt ttttcttaat ttaaaaaaga
    50221 agataacctt ttttcaaaac aacagctttt attgctgcaa cgacttctgg aatcgatttg
    50281 ccagtcgtat cgatacgaac agcatcctct gcttgcttca gcggagagac gtcccttgtt
    50341 gaatcataat ggtctctttc ctgtatctct ttcgtcagtg tttcaagatc agtcggaatt
    50401 cctttttctt ggttctcttt gaaacgtctt tctgcacgct cacttacgct tgctactaaa
    50461 aagattttca cttctgcatt cggtaataca gctgtcccga tgtctcttcc gtccatgaca
    50521 acgccaccgc gttcccctat ttttcgttgg acagcaacta attcttctct gacttttcca
    50581 tgtgcagaga cttcagaaac agcttttgtg acatctggtt gacggatttc taaagtcaca
    50641 tcttttgatg ctatgaatac atgctgtcca tcttcttgtt gcgaaaaaga gataggatac
    50701 tttttgatca gcgaaactag cccttcttca tctgaaaaag atacattgtt cgtaatagct
    50761 aaataagtta ctgcgcgata catggcaccg gtatctgtat aaatataatt caattctttt
    50821 gctaatatct tggcaacggt acttttccca gatgaagcag gcccgtcgat tgcaatactg
    50881 atttgtgtca tgatataact cctttttctc agaaaaaaag ccgcaaagcg cggctattat
    50941 tatttaattc ttaattcttg accagggtaa agcataaagt tgtttggatc taagccattc
    51001 aattcaaaca attgatcagt ggtgatccct gcacgctcag ccacttgttt tggaccttcg
    51061 cctgattgaa cagtcgttgt tgaaccggct gcagctgatg aagaaggtgt tgtttctgct
    51121 gcattgctgc ttgaatcccc tgcttgataa gttgcctcac ttgaggaagt cggtgttgtc
    51181 tctgctgcaa tactgctttc tgacgttaca cctgtatctt gagacacagt agattcttcc
    51241 gcgattgtac tagattctac tgttgaagtc tcagttgact cctcttttgt tgtacttgta
    51301 gaactttcta ctactgatgt cgaagtttca gaagaagttg ctgctgttgc attgttgctt
    51361 gatccatttc tgatccagaa ataagcacct gctggaatag cgatacataa gaccagtaaa
    51421 atcaaaaata gcgaaagaaa taatgtattt cctttctttt gttgtctctg agaacttctt
    51481 gatgaaactt catcatctgt atcgtagatt ggttgttccc atggttcttg tgtttcgtta
    51541 ttttctgtag attgatgtct atcttttctg ctcactgttt tacctcctaa actctatcgt
    51601 gtgtattata ccataggaaa aagtgtcttg tcctaaaaaa tcaatgttcc atgttttttt
    51661 taaataaatt tagtaaaatc gtttcccaat gaagttgttc agtcttagaa aaaagcttcc
    51721 tctttggcag aagttctgct tttgctccat caatatcaca acaattctgc ggcttttcgg
    51781 agagtttttc gccaaaataa gctaagatga attttctgcg gcagttttct tcattaatat
    51841 agcgaagcat ctgctgcagg cggaattctt tttctttttc ctgacgtttg agccgttcca
    51901 cccaattttc tggtttttca gactgctgga tcagctccat ccattttttt tgcagctcat
    51961 caaagggagc ttgctctgtg gcatattcca aataaagctc aaaactttgt cgctgttctc
    52021 tagaaagttg attaaagaaa taatggatac gttcatcccc actttgataa aaaagaattg
    52081 ctgcactttc ttcctgatct ctgcctgctc gaccgatttc ttgtacatag ttttctagac
    52141 tgtctggcaa atcataatgg accacataac ggatatcaga tttatcgatc cccataccaa
    52201 atgcattcgt agcaatgaga aactgcagct gattttttac aaactgctgt tgaagttgtc
    52261 ttctttgagc tgcatcaaga ccaccgtgat agtatccaac tgtaaaacgt tccctgaata
    52321 aatgatacaa tcgttcgact tctttttttg ttgcacaata aatgattgcc gctccttgtg
    52381 ctctttccat gaagtgttca agttcttgtt ctttttgctg cgttttccga acaaaaagcg
    52441 agatattttg ccgattgact gattgtctga tctcttttgg tggtcgatca aatagtacat
    52501 gacgtatatc tcttgctact aaatctgttg ctgtcgccgt tagtgccagt gtgaccgggt
    52561 ttcctaaaag ctttcggatt tttcctaact gttgatattc tggacggaaa tcaactcccc
    52621 attgagacac gcaatgagcc tcatctacta catataacgc aatattttgt tttttcagat
    52681 gctctaaaag agaaggatta gtcaacattt cgggactcaa aaacaaaaat ttatattgcg
    52741 atagatgacg gaggacgtat cttctttcag attgcgaaag taaactattg aaagcagcta
    52801 ctcgtttctc ccctcgtttt tgtaattgcg tcacttgatc ttccattaga gaaagcagcg
    52861 gtgacacgat gatgaccaga ccttccatta aataccccgt caattgatag caaaggcttt
    52921 ttcccgttcc tgtaggcaaa atagcgagag tatcttctcc atcaagcaac gcctgaataa
    52981 tttcttcttg gccgggacga aatgaatcaa atccaaaata tttttttaat tcatttatta
    53041 acattctgta tcgcttcctt tctcaataga tagatttgat acaaacgaaa ttctccataa
    53101 tctaattgtc catctacatt ccattcgcta aagcgccact ctcgcacgga atccttattt
    53161 ggttccaaac gttcgattgt ttctgcagag agaatccact cgtatggaaa atcatcaaaa
    53221 aacaaggccc actcaataag atgatctgtt actgttcctc tttttaaatg acggattgcc
    53281 atgatctggt caattgtctg accattcata aacatctgct tggtgatcat catgctttga
    53341 ttgaaatttt gctgtaataa tgaatcgatc aaagcgtgcc aaagcgaatc tggccgtttt
    53401 tcgatttgtg ctaacaaaag gtggatacat ctgctttgtt gcagataaac cgctgactca
    53461 tcattgtttt ttgccagctg ataaggaagc agccctgttc gctcatttcc agataattgg
    53521 ttagctaaaa aatctgctgc ttcttctggt atctccgaaa acagctgcac caaactctca
    53581 tacgcagtat caatcaaaat ttcacgtggc aattggcttt cactaagcca tttttttagt
    53641 tggaaagtat aaaaaggact tgtctctgct ggaagatagt cttgaatacc cgttgccaga
    53701 ttagaaatta cttgaacagc aaatttaatg agccgccaac tagtttcccc tgttctgcca
    53761 tagcggtcaa aacgcaaacc agtatgttct atttgatgtt cggacaacat atcagcccct
    53821 gcaggcgtta gctttgcttc attttcattt atttcgatcc aaccttgctg cgcaattttt
    53881 tgcaaaattt gataaaactt atcctgcttc aattctggaa aactgttatg gataaacaaa
    53941 agttcgtggg taaatccata aataagtact gaagtcgttc gttttcccac aaggagttgg
    54001 taaagagaac taatccgcag cttgtgtcct gatgaaaata gcgataaaat aaaaacaatc
    54061 atattattct ccttaagtta ggatggttag aaatgtcact actatgtaaa cttgtacctg
    54121 aacgctgtat tgcatgcggt ctttgtcaaa tcgaagcgcc agatattttc gattatgatg
    54181 aagaaggaat cgttttattt gcccaagaac cagaggctca tcaacagttc gtgatgccgg
    54241 aagaagaaac atatgtaaaa aaagcgtatc aaaaatgtcc agttcgagcg attttattag
    54301 aaaataaaat ataaatataa acgaaacttt aaaaacgtac actaagttct ctaaacttga
    54361 aaataagccg ggatctgttt ctcttgttcc gtgaaatccc gacttatttt gaaagattca
    54421 tttgctatct atagttaatt gactagtttt ttcacggacc ctataggacc catttatatt
    54481 atttcaactt tttcagccag ttttccaatg tattgacgat cacagtttgc tggtctgcgt
    54541 tagaaatttc tgcctctcca tctccttttt gctcaccata gctgccaaaa cctccatgat
    54601 ttccgccatt gattgataca tactctgttc tttccggtaa atactgctta gcttcttgat
    54661 aagcttcctg attcaatact ttatcttttg tcgccgtgat cgataaaaca ggaacagctg
    54721 attctgtcaa tgttcctttt ttgtcgggat aacttgccat gaagaatacc ccttttaaat
    54781 gtttgtctga atgattctta ttgacataac gacttgccat tacgccacct aatgaatggc
    54841 caccgatcac ataatcggaa tttggaaaat cttctttgac cttatccgct ttgtttccgc
    54901 ccatcactgc tagatcaagt ggcattttga caatagcgac tgaaaagccg ttttttgcta
    54961 aatcttgcgc ccaaatacta taactttctg gctctacgag agcaccagga taaaaaataa
    55021 gtaatggaga ctttccatga ttctttttct ctccttcaaa aaataagtaa tcttctttgt
    55081 tttttccttc ttttaccaac acttgcgctt ctgacgatgg ttcgtacgta ttttgctgta
    55141 tatagtaata accggctcca ccgagtaatc ctaaaatgat gactaagcca attagtattc
    55201 gtctaaatat tttcactttc tcccaccttt tctatctgag tgaccccttt ttcttaatca
    55261 agtatgatta taccttaaaa aaataagaga gaaatgcaaa ctttccagca tcaaataaaa
    55321 aaagacggaa tatccatttg aatatccgcc ctttttgtat gtttattttt atttagcaca
    55381 taaacgatac acagcttctg cataaatttc cattgcttta tacatactac ttaattccca
    55441 tcgttcattc gcctggtgca taaagtcagg agtatcaggg aacatcgcac cgaatgccac
    55501 acactggttc atcgtacgag caaaagtagc gccgcctgat acaaacggtt cagtcatgtc
    55561 tccagtaata tcgcgatatg tccccaatag atttttcact aactcactgt ctagtggcac
    55621 gtataatgaa tcaaggaagt caaattcttc ataagtcaac ccatattttt ctgctgtttt
    55681 agttaattta gctactaaat catctttctt gaatgtaaca gggattctca tatctacacc
    55741 gatttttgtt tgttctggtg tgatttccaa acttgcaaag ttcatcgtta gctcgccgga
    55801 ttgttcatct tctgttttgc ccacgacagc ttcaccagtt gcattttctt gaactaattt
    55861 tcctaagaaa ttgattgggc caaaatcaaa tacttctgaa agagcaattg ccagacgaga
    55921 aatagcattc actccttgtg ctgcatcttt cgcgtggata cttttaccaa gaacgacgat
    55981 gccttcacct tgttcttcga aatcgaattc gtgttttttc aatgcttctt ttacttcaga
    56041 aagcttttct ccagagtaag gagctgcatc aggaacaaca ttcaacgctc ctccagcttt
    56101 tacagaaaac tcgttcgttc ctggtcctgt caagtaagct tgcaataagc ctttttctgc
    56161 ataaattaaa ggaaattcag catctggcgc aaatccttgc gtaattcctt cttctttttc
    56221 gttatatttt tctaaacaac gccataaatt ttcttcatct gtaccaaaga taaaacgaat
    56281 acgtgtcttg aattccacgc cagcatcaat caaagctttg acggcataca ttgccgcaat
    56341 agaaggacct ttgtcatctt gggatccgcg accaactaac cagccgtctt ttatagttgg
    56401 atcgaatggt tttgtttccc aattattttc gtcccctgct ggaacaacgt ccatatgaca
    56461 caagataccg aataattcat ctccagagcc aatctctgaa taaccgtaat agccttctgg
    56521 atctttaaat gttctaaatc ctagcttttc actgatttcc aatactttat ccaaagcttc
    56581 aatcactttt ttaccgaagg gatgtccttg tcctgaatct gcttcatcta gtacggaagg
    56641 aattcggatc aattcgctca atgattctaa tgcttgttca tgatgttgtt ctgtcacaaa
    56701 ttttttcatg atattatcct cctaatatgt taaaaagggt catgtcaaat tcctccgaca
    56761 caacctcttc ttctcaaata atgacgtttt aagcctgctc catcagcaag cagaatgttc
    56821 ttaacaaatg atgaaagaaa aaacactcac tgaatggagc tattgactta tccacgtgct
    56881 tatcgatagc cgtttatcgg tttcttcatt cttatgatag accttgtacc tgtatttgtc
    56941 taggtttaga acattgctgc tacacctagg atcacacaga tggctacaaa taatatcgca
    57001 attaatttac cggtaaattt ccaccacact gaaacatcga aacgagcaat tgctacagca
    57061 cccattacga tagcagatgt tggtgtaatt aagtttacta aaccagatgc agattgataa
    57121 gcagtgatta ctaaatcctt accgacgccg gcaaattcac ccataggtcc catgattccc
    57181 attgttgcag cagctaagcc ggaagttgaa ggaatcaaga atgacatagg gatatagaaa
    57241 ataaatgtaa taatgatgaa tacacttgaa gataatgaac ttaatccttg ttcgccccaa
    57301 tgtaagatgg tgtctgtgat taatccattg ttcataacca cttggatacc acgagcaaca
    57361 gctactacga ttgctacacc gatcaaatca gaagcaccag ctaaaaattc agagacaaat
    57421 tcagactctt tcatgccata agtgattgct actacgatgg acattactaa gaataacata
    57481 gtaatttctg ggaagtacca gtcacctaat ggaagcatgc ttttacctag aaccgtacct
    57541 agtaccggaa ggtttgttaa ccagtttgtc aatgattcaa aaatcgtgaa attcgcattc
    57601 aaggttgtcc aaggaatcaa actgatgatc atgataccaa aagtaataaa gaacaataca
    57661 ttaacatgtt tttgtttttt cgtgattact tcttgacgac cgattgcttc aatatcgaaa
    57721 tgcttcatat cttctttacg ttggttgtat actaatgatt ttgttggatc tttttcgatc
    57781 tttgatgcat aacgatacac atatacaata gaaatagcta acaaaataac gaacatcaag
    57841 aatcgaagac caataccttc acccatcgaa atcccaacag cttgtgaagc tacgcctgtt
    57901 gcaaaaggat ttaccgttga agctagacag cctacttgag accctactaa tacgattgct
    57961 acagctacta ctgtatcaaa gcctacagcc atcatgactg ggatcagtaa agcatagaat
    58021 ggaatcgttt cttctgccat tccgtaagta gagccgccta atgcaaataa cagcatcaat
    58081 actggaataa gcattttttc ttttcctttg aatttcttca caactgaagc aatcccggca
    58141 tctagtgccc ctgttttgtt gataatacct aaaaatccac caacaaccaa aatgaagaaa
    58201 gagattggga ttgcagcagg tgtttcttta gttagctccc ccgctggtac acctagcatc
    58261 ccattgattg gcgccatgaa aatatcccac agcccttgag gattttgttg tactcgttca
    58321 tatgatccag cgacgatgtt gcctgcatcg tctacactat agtgcccagc tggtataaac
    58381 caagtaagta cagcgattac tgcaataata ataaataaga ccgtatacgc tgaaggcatt
    58441 tgaaattttt tcttttttgt ttgttccatc ttcttcttcc tttcttttcc gttagagaca
    58501 acaattttgg ctctaatgtt attttatcaa aaagagtatc agaacaaaaa taataacacg
    58561 cattttgtaa aacttttgtt caaaaagttc gtttttccct attccctcct cttttttcag
    58621 gtttttaaag ctttcttctt tttcattgta gcgtttcctt ttttttataa acaaacattt
    58681 tcgtctttta aaaggagaag aatttcacat ttgcaaagaa ttgattgatt tttatgcata
    58741 aatcaaaaaa atactcaaaa caaacaaata aaacgtttac attattgcat gtcttgtatt
    58801 tttttccaaa aaaaaagacc aagaaatttc ttgatctttt tgatatttat ttgactgctg
    58861 tatgacgttg ttcgaactga tgttgttttc gacttaacca tggaagaagt tgcgcatgca
    58921 gcactaaaaa gacagcactc acgatgaatc ctttgatcaa gttgaaagga acgatcccaa
    58981 tcagtacata gtttgctaaa gacatgccca gcatctgtgt tgcgttcagc cctaaaaaca
    59041 ttaagtaaag aggcgtaata acgaaataat ttgcgatact cataaaaata gttagagcga
    59101 tggttcctgt aatgacacct aaaaatttgt ttcttccttt atgctgtttc tttacactaa
    59161 agaaataata aatcgggaat gtaaagatcg ttgttgataa gaaactagca gtgtctccta
    59221 ccaaattatc cggagaaaaa cctgtagtca gcagatgtaa tactgaacgc aagaacgctg
    59281 ttgcaactcc ggccgctgga ccgaataaga acatgctgat caataccgag atatcactaa
    59341 aatcgatctt caaaaaacta aaagccggca tgatcggaaa agcaaaatac tgtaaaacta
    59401 atgcgatcgc agcaaacatc gctactgcaa ccattttctg tacacgggtg ttcttcattt
    59461 gaaatcctcc tgttaggact cctcttcagc gaactttttg acgtttctct ccaaatgaaa
    59521 aaactctatt ttccagggaa aatagagtta gagttatttg atcaagcgtc gtcaaataag
    59581 ctctatcttc tttatccaga ctatactgtc ggtatcggaa tttcaccgat tcagctattt
    59641 ttttgcaaaa taggtcgtgg actataacca ccggtcggga atttcaccct gcccctgaag
    59701 acgaacctta tataattttt gtttacatat tcattataca tatctattca aaaaacgcaa
    59761 catctaacac ttataaaaag taagtgttca ttttgagtgg ttcagcaaac tgctgacttc
    59821 ttttttgttc agttcacggt aatccccagg acgtaggcct tgtaaagtca gatctccgta
    59881 tttttcacgt tttagttttt gcacaggaag accaactgct tgaagcatat ttttcacttg
    59941 atgattacgt ccttcgtgga tcgtcaattc cacgatactg tggtttgtct taggatccac
    60001 tgataaaatt tggaatttag ctggagatgt ttttcgtcct tcgatcttta ttccttttgt
    60061 caaaggtgca agcatcgatt ttgtagcgat cccttttacc tttgcaacgt aaactttatc
    60121 cacttcatgt tttggatgtg tcaggcgctg agcaaaatct ccatcatttg tcagtagcaa
    60181 gatccccgaa gtatcgtaat ccagtcgacc tacaggatag atacgttctt ttaccatcgg
    60241 caaaagatcc gtaacaactt ttcttccttt gtcatctgca acagcagaaa taactccttt
    60301 aggtttgtac aataaatagt aaccgtattc ttcttgataa attgggactt catccacttc
    60361 cacagtgtca tgtttcccaa cttttatccc taattccctt acaacttgtc cgtttacttt
    60421 tacacgtcct gcaaggatga attcttctgc ttttcgacga gaagtgatgc cagcgtgggc
    60481 aattactttc tgcaatctct ccattacaat tattctcctt tattcattcc atcaaagaat
    60541 agatctggtg acacgtcttc ttcaagtgaa atttccattt cttgtgtatc aggcagttct
    60601 tctaatgatt tcaaaccaaa ataatctaag aagtagccag ttgttccata taatatcggc
    60661 cgacctggtc cttctactct acctttttct tcaatcagtt tatgggcagc tagacgctgt
    60721 accgctcctg aaacctgaac tcctcgaatc tgctcgattt cagctcttgt gattggctgt
    60781 ttataagcga tgattgctaa ggtttcgacg gcggcttgag ataatgtgtt tgatgtccct
    60841 tgcgcatatc gcttaagtaa gggagccagc tcttttttag tcgttaatac ataatggctt
    60901 tccgtttcaa aaatatgcaa agctgagtgc gagttttgag tgtaagattc ttttaattct
    60961 ttcactaaac ttgtgacttc tgcttcgctg agatttaaca gtccgcttaa ttcttcatta
    61021 ctgatccctt cctcgccaac aataaaaaga agggcttcaa gttcacttaa tttcgtcatg
    61081 ttcggtatct cctactgctg gataaagcat aattgattca tagttttctt tttgttgcgc
    61141 aatgatctgc ccttttttca tcaattctag caaagccata aaagtagtga caacttcact
    61201 tttagaaggt gactcaaaaa aagaatcgaa agtacagcca tgcgcatttc tttttatacg
    61261 ctcttcgata aataccattc gttcttcgat cgttgtgtca tcgggcgtga tcgttttttc
    61321 cactttttga gaaaatttct tacgttctaa gattttctgc atggcacgga tcaaatcgat
    61381 ttttttgact tgtcctcgct tcaacggctt ttcagcatcc gctaacccat cgatgttcat
    61441 aggttctttt gtgtaataaa gacttcgttc ctctgctttt tctgtcagtc tttcagctgc
    61501 atatttatat ttacggtatt ctaaaagctg agtgaccaat tcttctcgag gatcttcttc
    61561 ccataattct tcatcgtctg tgacgaattc ttggacagga agcaatgtct tgcttttgat
    61621 tgccatcaat gtagcagcca tcactaagta ttctcctgca acagctagct cgagtgtttt
    61681 cattgtatgg atataattca tatattgctc cgtgatcgta gcgatcggta tatcatatat
    61741 atcgatttcc atttgctgaa tcagatgaag cagcagatcg agaggaccct cgaatacatc
    61801 taatttaata ttgatctccg tcaactcatt cactctttct tctttttctt tgctagccat
    61861 tttccaatgt aaggctctgt gatttcagtt gcacggatgg agacatccgg gtacttttcc
    61921 tgtaattcat ctaacatttg aaagcccata ttctcaccgc gataagacgg attcaaagaa
    61981 atgtcatgga tgatcagctg atcgttcgtt gcccagtaaa taccaataat cccttgaatg
    62041 ttggttgaac cttctggcgt aaagaaaaaa agttcataat cgtcttcaga ttcgtacgta
    62101 tcaatctcct tcaacaaaga agttttacct gtcagtttct tatggaaact caatagtccc
    62161 atagcaattt tacggttttc tttccggtaa tgtgttaaca tcttctcacc ttcctatgac
    62221 taagcccttg gaaaatgctg cttataaaca tccgtcattc ttttcttcgt gatatgtgta
    62281 taaatctgag tcgtagagat atccgcgtga cctaacaatt cctgtactgt ccgcaaatca
    62341 gctccatttt ctagcagatg tgtggcaaag ctatgccgca gcgtatgtgg tgttacttct
    62401 ttgtaaatcc cagcctctcg tactaactgc ttcagatttt tccagattcc ttgccgagaa
    62461 agacctttcc catgattatt gacaaataca tgcatttcag atgcgtcctt tacaagtaat
    62521 ggacgtgctt cgtctaagta gcgttccagc cattggatcg cataatcacc taaaggaatg
    62581 atccgctctt tatctccttt tccaactgtc tgtaccagtc caagagaaag atgaagatct
    62641 cccaatttca atccaatcaa ttcactgacg cgcatcccag tagcatacat gacttccaag
    62701 attgcacgat ctcttatccc taacgttttt gtcgtatcag gtgcttcaat cagcttttct
    62761 acttctgtca gagtcaatgt acttggtaat ttctgtgctt tcttcggcgt atcgatatgt
    62821 tgcatcggat catgatcagt gatacgctct tgccgtaaaa actgatggaa tcttctgaga
    62881 cttgagatca tccgagtaac agttgctgtc gctttttttt cttcatgcaa cttctccaga
    62941 aaacggatga ctaaaaagcg atcgacatcc tgccatgaag caattttctg ctcctgtaga
    63001 aaatgcaagt actgttccaa atcacgttca tagctttgtc gtgtattggc ggacaatccc
    63061 cgttcaatat tcaagtagtg gagataatcg ttgatttgct ctttcataat aatccccctc
    63121 atgcctctct atcatatcag ataccgcttc attctgatag tctttttcca taatctgagc
    63181 aaagaaaaag ccgtctagac actcagacgg actggtttct tttttctttt tcatagcatt
    63241 cctgacagat tccatggaac gttagccgat gatcttttac taaaaaatga taacgttctt
    63301 ctactacttg ctccacttca agtaaaagat cctcttctac ttcctcgatc gtgccgcagt
    63361 tcaagcatag taaatgatga tggaaatgtt tggcgccttc ttttctcaaa tcataacggg
    63421 ctaatccatc attaaaactt actttatcga caattttcaa ttctgtcaag atttcaaggg
    63481 ttcgataaac agtagcaagt ccaatttccg gactcttttt tttcactaaa aagaagattt
    63541 cttctgcaga taaatgctct ttctcatttt ccaaaagcac aagcagagta gcttctcttt
    63601 gcggtgtcag cttaaacccc gcttcatgta gctgctgttt tgtttttttc atcgccgaaa
    63661 tagcattcat gaccatttac tcaaccccaa caatttataa taattacaaa taaaaaaaga
    63721 tataacacta tgttaattaa aattattata agctagttaa gaaaaacatg caagggattc
    63781 tcaattagat tttttgacta gttttgtcac accgttttct tgtgtaatca atccttgttt
    63841 caaaagatta cctaatgcgc gcttaaatgc tcctttgctg ataccaaaag ctttcatgat
    63901 ttcttctgga tccgatttat ctgtaaagtt gatttgctga tctgcagaac gttctaaaat
    63961 ggttaaaatc atttgtgcat catctgatat agcttcatat gctcttggct tcaatgaaag
    64021 attcagtaca ccgtctggtc ggacaccaat cactcgacct tttatctgct cgcctagacg
    64081 aggttcatta tatcgttcag aaggatggat aaacccaaga tagaaatctt ccgttaaaac
    64141 gtacgtacct gttaatttca agcggtatgc tgttcctatc acatctttgt ttttcatttc
    64201 ttctgttcca tatttgctta aagatttgaa tattttttca tctgctaaac tgccccaaat
    64261 acggtctttt gcatcaactc taagggaaat catcaagcga tccccttttt taggccatag
    64321 ctctgtcatt gtcggcaatt catcaagaga tacgacaaag tctttatctg gaagaccgat
    64381 atcgacaaac gcacctaaat cttttcgtgt agcagtaacg attccaaaac cgtaatgacc
    64441 gatacggctt ttaggaattt gtgtagtgat ccgattttca tgtttttgat tttggtaacc
    64501 gaatccttca actgcttcac cgattttgtg ttcaccttct gatttgctta agccaaaggt
    64561 ttgtccgttt ttttggacaa agtagttttt ttcattttca tcaataataa tacctgtaaa
    64621 tatagttgct aataaatcgt tcattttttt ctccctctat agtgaaaggt tttcttaata
    64681 catttctttg ctatcataac ataatgttat ctacttgaat agctgagcct tggtttggaa
    64741 aggtgaaaaa aggagtatga caacagttgt gtcatactcc cctttttcga ataaacggtg
    64801 ttaaacggca ggctcacagc ctctttcatt tgcctaaaat taattgctcc catttcctcc
    64861 gaaccatcca gttttacagt tgatggttac gatgtaaaat ggcaaagcac tttctccaat
    64921 gcctttcatc aatgcagatt ttgttgaagt ggcatataga ttataatagc cttccccatt
    64981 ttcgattctg gctttttcta atctgtaacc tccttcaacg atatatccac gttggataca
    65041 tgtagcaatc actttgcttt ggacacctgg tgcccacgtc catgctggat tagatgcatc
    65101 ggcaatatca cttgctctgg cagatttgat tggctgattc gctgagtttg aagaggaagg
    65161 ggtcgaggat gccgcttctt gctgtgctgc ttgtgcgacc gcagcttgtt gagcggcttg
    65221 agctgcagct gctgcttgag cggcttgttt tgcttgttct tcagctgctt ttttcgcttc
    65281 ttctgctttt ttcttttcct cttcttcttt tttctcttta tcagtgagca tcttatctac
    65341 ttgtttcaat tgttcattca actgagcaga tagatctgta tttttcactt tttctactgc
    65401 ttcttttgcc gtcttgtatt ggtcgcgtgt tgctgaatcc actacttttc cgtctttaaa
    65461 gactatagct gttttttctt tagctgtttg caattcagca tattgatttt tagcttctgt
    65521 ttgtgcttgt tgaatcaatt ttccaaaatc tgtgttatcc gtttcgattg cttggatctc
    65581 tttatctgct ttcaacttga tgtcatctgc tagatgatca tcgacgatgg caggtgctat
    65641 gaaccactca ttcacttttt ggatatctcg gatcttagat tggatatctt ggtaagttgt
    65701 ttctaactct gaatatcctt tttcattctt aagactggat aaatcatttt ttagttttga
    65761 taaatcttga tgaatcatat ctgcacgaat aaattgatgg tgatcatctg tataaaaggc
    65821 agcaagtttc tcatgaagtt cttccatttg ttgctgtctt tttgcttctg ctgctacttc
    65881 ggcttctact ttttgatgat ggtcataata agcccagctt cctccaccga gtagaagtac
    65941 tgctgctaaa acgagataag gtactttttt tgattttttc ttttgcgctt tatctggatc
    66001 tgttttttct aagtttgatt cttgatgtgt tttttcagta tctggcacga ttgttattga
    66061 tttcgcttct gcttcaaaca gcttaggctg gttctcttct ttttctattt ttgcttcaag
    66121 atcagtaccc tctttgctta cagattgatt ttcagcatcg gattcactat ttatttcatc
    66181 gggctctgtt gactttttgt ctgtgttttc gagtatttct tttttctctg cttccggcag
    66241 tttattcgtt ttttcctttt ctaattgttt ttcagtaaat acttcagttt tttctgtgat
    66301 atccgttttt acgtctttta tttttactgt ttctttacct gtggcgtttt ctctatcaga
    66361 gtctttttca gtttcttgtt ccttttctgg ctctactttg ttttcctgtg caattttttc
    66421 actgatcatt ttgagcagct ctttcgcgtg ctcttctttt gtatcttcac gatgtgcttt
    66481 gatataagcg gatagtatcg aattttcttc ctcattatcg acgatttctt tcagttcagc
    66541 tactcttctt ttttgttttg attcaagcgt ttcttcttga ttctttcctg attcttccga
    66601 agtctcatga acagttgctt tcttctgttc atttttctct atattagtta cttgatcgtg
    66661 agcaggtggt tcttcgtctt ctggttctgc tttttcgaat aattccatta cagattctag
    66721 aggtaaatct ttcaattccg accattcgat tggatcgttt aattctgtat caggaaagac
    66781 tgcatcttca gttgatttat cctgcatagc tgcatctgta tttgtttctt caattgattc
    66841 ttttatttgc tgatcttctg cagttatttg atgaccgcag tctggacaga tagtattctg
    66901 ctccattttt ttcgatccgc aatttggaca ttttttcatc acttagcgat cggctccttt
    66961 actaggttta cgctaccatt taatctatca tattctatac gtcacgccaa tagcttattt
    67021 ttaggattta cttaaaaata atgaatgaat ttccttccta tagaaaaaaa agcttgagac
    67081 tgcgtagtct caagctt
//
LOCUS       DAZKKW010000004        66172 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.004, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000004 DAZKKW010000000
VERSION     DAZKKW010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 66172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 66172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..66172
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.004"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            476..1339
                     /locus_tag="ACG8PO_000197"
     CDS             476..1339
                     /locus_tag="ACG8PO_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFQ0205556.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(1468..3090)
                     /locus_tag="ACG8PO_000198"
     CDS             complement(1468..3090)
                     /locus_tag="ACG8PO_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFQ0205557.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            3339..3860
                     /locus_tag="ACG8PO_000199"
     CDS             3339..3860
                     /locus_tag="ACG8PO_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HFQ0205558.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(4046..5776)
                     /gene="spxB"
                     /locus_tag="ACG8PO_000200"
     CDS             complement(4046..5776)
                     /gene="spxB"
                     /locus_tag="ACG8PO_000200"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047112 - pyruvate oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HFQ0205559.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            5941..6759
                     /locus_tag="ACG8PO_000201"
     CDS             5941..6759
                     /locus_tag="ACG8PO_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HFQ0205560.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(6862..7059)
                     /locus_tag="ACG8PO_000202"
     CDS             complement(6862..7059)
                     /locus_tag="ACG8PO_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0205561.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            7228..7806
                     /locus_tag="ACG8PO_000203"
     CDS             7228..7806
                     /locus_tag="ACG8PO_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HFQ0205562.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            7901..8038
                     /locus_tag="ACG8PO_000204"
     CDS             7901..8038
                     /locus_tag="ACG8PO_000204"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0205563.1"
                     /translation="MDQKRIHVGLITSFVTMLIYLDQGHLTWTVVGVVGFLTLFTAIK
                     K"
     gene            complement(8851..9354)
                     /locus_tag="ACG8PO_000205"
     CDS             complement(8851..9354)
                     /locus_tag="ACG8PO_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFQ0205564.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            9713..10738
                     /locus_tag="ACG8PO_000206"
     CDS             9713..10738
                     /locus_tag="ACG8PO_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFQ0205565.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            11061..11939
                     /locus_tag="ACG8PO_000207"
     CDS             11061..11939
                     /locus_tag="ACG8PO_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase domain-containing protein"
                     /protein_id="HFQ0205566.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYHFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            12415..12531
                     /locus_tag="ACG8PO_000208"
     CDS             12415..12531
                     /locus_tag="ACG8PO_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HFQ0205567.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            13459..13797
                     /locus_tag="ACG8PO_000209"
     CDS             13459..13797
                     /locus_tag="ACG8PO_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0205568.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            13891..14121
                     /locus_tag="ACG8PO_000210"
     CDS             13891..14121
                     /locus_tag="ACG8PO_000210"
                     /inference="COORDINATES: protein motif:HMM:NF042636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6568 family protein"
                     /protein_id="HFQ0205569.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            14521..14892
                     /locus_tag="ACG8PO_000211"
     CDS             14521..14892
                     /locus_tag="ACG8PO_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HFQ0205570.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(15166..15858)
                     /locus_tag="ACG8PO_000212"
     CDS             complement(15166..15858)
                     /locus_tag="ACG8PO_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFQ0205571.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(15861..16646)
                     /locus_tag="ACG8PO_000213"
     CDS             complement(15861..16646)
                     /locus_tag="ACG8PO_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFQ0205572.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            16883..18283
                     /gene="lacG"
                     /locus_tag="ACG8PO_000214"
     CDS             16883..18283
                     /gene="lacG"
                     /locus_tag="ACG8PO_000214"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033920 - 6-phospho-beta-galactosidase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HFQ0205573.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            18308..18625
                     /locus_tag="ACG8PO_000215"
     CDS             18308..18625
                     /locus_tag="ACG8PO_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFQ0205574.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            18638..20311
                     /locus_tag="ACG8PO_000216"
     CDS             18638..20311
                     /locus_tag="ACG8PO_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HFQ0205575.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            20325..21956
                     /locus_tag="ACG8PO_000217"
     CDS             20325..21956
                     /locus_tag="ACG8PO_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinogalactan endo-beta-1,4-galactanase"
                     /protein_id="HFQ0205576.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(22447..24012)
                     /gene="guaA"
                     /locus_tag="ACG8PO_000218"
     CDS             complement(22447..24012)
                     /gene="guaA"
                     /locus_tag="ACG8PO_000218"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFQ0205577.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVNARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(24071..24994)
                     /gene="coaA"
                     /locus_tag="ACG8PO_000219"
     CDS             complement(24071..24994)
                     /gene="coaA"
                     /locus_tag="ACG8PO_000219"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HFQ0205578.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            25190..25795
                     /locus_tag="ACG8PO_000220"
     CDS             25190..25795
                     /locus_tag="ACG8PO_000220"
                     /EC_number="2.1.1.172"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0052916 - 23S rRNA
                     (guanine(1835)-N(2))-methyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFQ0205579.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            26103..27119
                     /locus_tag="ACG8PO_000221"
     CDS             26103..27119
                     /locus_tag="ACG8PO_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0205580.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            27260..28561
                     /locus_tag="ACG8PO_000222"
     CDS             27260..28561
                     /locus_tag="ACG8PO_000222"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016154 - pyrimidine-nucleoside
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HFQ0205581.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            28607..29269
                     /gene="deoC"
                     /locus_tag="ACG8PO_000223"
     CDS             28607..29269
                     /gene="deoC"
                     /locus_tag="ACG8PO_000223"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HFQ0205582.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            29290..29688
                     /locus_tag="ACG8PO_000224"
     CDS             29290..29688
                     /locus_tag="ACG8PO_000224"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HFQ0205583.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            29754..30836
                     /locus_tag="ACG8PO_000225"
     CDS             29754..30836
                     /locus_tag="ACG8PO_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HFQ0205584.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            31048..32610
                     /locus_tag="ACG8PO_000226"
     CDS             31048..32610
                     /locus_tag="ACG8PO_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0205585.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            32603..33736
                     /locus_tag="ACG8PO_000227"
     CDS             32603..33736
                     /locus_tag="ACG8PO_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0205586.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            33736..34692
                     /locus_tag="ACG8PO_000228"
     CDS             33736..34692
                     /locus_tag="ACG8PO_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0205587.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            34791..35957
                     /gene="deoB"
                     /locus_tag="ACG8PO_000229"
     CDS             34791..35957
                     /gene="deoB"
                     /locus_tag="ACG8PO_000229"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFQ0205588.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            35981..36799
                     /locus_tag="ACG8PO_000230"
     CDS             35981..36799
                     /locus_tag="ACG8PO_000230"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFQ0205589.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            36816..37523
                     /gene="deoD"
                     /locus_tag="ACG8PO_000231"
     CDS             36816..37523
                     /gene="deoD"
                     /locus_tag="ACG8PO_000231"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFQ0205590.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(37821..38507)
                     /gene="gpmA"
                     /locus_tag="ACG8PO_000232"
     CDS             complement(37821..38507)
                     /gene="gpmA"
                     /locus_tag="ACG8PO_000232"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFQ0205591.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(38679..39260)
                     /locus_tag="ACG8PO_000233"
     CDS             complement(38679..39260)
                     /locus_tag="ACG8PO_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0205592.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(39393..40076)
                     /gene="rpiA"
                     /locus_tag="ACG8PO_000234"
     CDS             complement(39393..40076)
                     /gene="rpiA"
                     /locus_tag="ACG8PO_000234"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFQ0205593.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            40395..40808
                     /gene="rpsL"
                     /locus_tag="ACG8PO_000235"
     CDS             40395..40808
                     /gene="rpsL"
                     /locus_tag="ACG8PO_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFQ0205594.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            40892..41362
                     /gene="rpsG"
                     /locus_tag="ACG8PO_000236"
     CDS             40892..41362
                     /gene="rpsG"
                     /locus_tag="ACG8PO_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFQ0205595.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            41456..43543
                     /gene="fusA"
                     /locus_tag="ACG8PO_000237"
     CDS             41456..43543
                     /gene="fusA"
                     /locus_tag="ACG8PO_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFQ0205596.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            43715..44902
                     /gene="tuf"
                     /locus_tag="ACG8PO_000238"
     CDS             43715..44902
                     /gene="tuf"
                     /locus_tag="ACG8PO_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFQ0205597.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            45309..45617
                     /gene="rpsJ"
                     /locus_tag="ACG8PO_000239"
     CDS             45309..45617
                     /gene="rpsJ"
                     /locus_tag="ACG8PO_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFQ0205598.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            45648..46277
                     /gene="rplC"
                     /locus_tag="ACG8PO_000240"
     CDS             45648..46277
                     /gene="rplC"
                     /locus_tag="ACG8PO_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFQ0205599.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            46305..46928
                     /gene="rplD"
                     /locus_tag="ACG8PO_000241"
     CDS             46305..46928
                     /gene="rplD"
                     /locus_tag="ACG8PO_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFQ0205600.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            46928..47218
                     /gene="rplW"
                     /locus_tag="ACG8PO_000242"
     CDS             46928..47218
                     /gene="rplW"
                     /locus_tag="ACG8PO_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFQ0205601.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            47257..48090
                     /gene="rplB"
                     /locus_tag="ACG8PO_000243"
     CDS             47257..48090
                     /gene="rplB"
                     /locus_tag="ACG8PO_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFQ0205602.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            48133..48411
                     /gene="rpsS"
                     /locus_tag="ACG8PO_000244"
     CDS             48133..48411
                     /gene="rpsS"
                     /locus_tag="ACG8PO_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFQ0205603.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            48434..48781
                     /gene="rplV"
                     /locus_tag="ACG8PO_000245"
     CDS             48434..48781
                     /gene="rplV"
                     /locus_tag="ACG8PO_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFQ0205604.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            48795..49451
                     /gene="rpsC"
                     /locus_tag="ACG8PO_000246"
     CDS             48795..49451
                     /gene="rpsC"
                     /locus_tag="ACG8PO_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFQ0205605.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            49454..49888
                     /gene="rplP"
                     /locus_tag="ACG8PO_000247"
     CDS             49454..49888
                     /gene="rplP"
                     /locus_tag="ACG8PO_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011375443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFQ0205606.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            49878..50066
                     /gene="rpmC"
                     /locus_tag="ACG8PO_000248"
     CDS             49878..50066
                     /gene="rpmC"
                     /locus_tag="ACG8PO_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFQ0205607.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            50091..50357
                     /gene="rpsQ"
                     /locus_tag="ACG8PO_000249"
     CDS             50091..50357
                     /gene="rpsQ"
                     /locus_tag="ACG8PO_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFQ0205608.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            50415..50783
                     /gene="rplN"
                     /locus_tag="ACG8PO_000250"
     CDS             50415..50783
                     /gene="rplN"
                     /locus_tag="ACG8PO_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFQ0205609.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            50820..51128
                     /gene="rplX"
                     /locus_tag="ACG8PO_000251"
     CDS             50820..51128
                     /gene="rplX"
                     /locus_tag="ACG8PO_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFQ0205610.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            51156..51695
                     /gene="rplE"
                     /locus_tag="ACG8PO_000252"
     CDS             51156..51695
                     /gene="rplE"
                     /locus_tag="ACG8PO_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFQ0205611.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            51714..51899
                     /locus_tag="ACG8PO_000253"
     CDS             51714..51899
                     /locus_tag="ACG8PO_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HFQ0205612.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            51935..52333
                     /gene="rpsH"
                     /locus_tag="ACG8PO_000254"
     CDS             51935..52333
                     /gene="rpsH"
                     /locus_tag="ACG8PO_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFQ0205613.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            52365..52901
                     /gene="rplF"
                     /locus_tag="ACG8PO_000255"
     CDS             52365..52901
                     /gene="rplF"
                     /locus_tag="ACG8PO_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFQ0205614.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            53062..53418
                     /gene="rplR"
                     /locus_tag="ACG8PO_000256"
     CDS             53062..53418
                     /gene="rplR"
                     /locus_tag="ACG8PO_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFQ0205615.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            53439..53939
                     /gene="rpsE"
                     /locus_tag="ACG8PO_000257"
     CDS             53439..53939
                     /gene="rpsE"
                     /locus_tag="ACG8PO_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFQ0205616.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            53954..54133
                     /gene="rpmD"
                     /locus_tag="ACG8PO_000258"
     CDS             53954..54133
                     /gene="rpmD"
                     /locus_tag="ACG8PO_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFQ0205617.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            54177..54617
                     /gene="rplO"
                     /locus_tag="ACG8PO_000259"
     CDS             54177..54617
                     /gene="rplO"
                     /locus_tag="ACG8PO_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFQ0205618.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            54617..55912
                     /gene="secY"
                     /locus_tag="ACG8PO_000260"
     CDS             54617..55912
                     /gene="secY"
                     /locus_tag="ACG8PO_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFQ0205619.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            55974..56621
                     /locus_tag="ACG8PO_000261"
     CDS             55974..56621
                     /locus_tag="ACG8PO_000261"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFQ0205620.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            56812..57030
                     /gene="infA"
                     /locus_tag="ACG8PO_000262"
     CDS             56812..57030
                     /gene="infA"
                     /locus_tag="ACG8PO_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFQ0205621.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            57063..57179
                     /gene="rpmJ"
                     /locus_tag="ACG8PO_000263"
     CDS             57063..57179
                     /gene="rpmJ"
                     /locus_tag="ACG8PO_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFQ0205622.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            57197..57562
                     /gene="rpsM"
                     /locus_tag="ACG8PO_000264"
     CDS             57197..57562
                     /gene="rpsM"
                     /locus_tag="ACG8PO_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFQ0205623.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            57590..57979
                     /gene="rpsK"
                     /locus_tag="ACG8PO_000265"
     CDS             57590..57979
                     /gene="rpsK"
                     /locus_tag="ACG8PO_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFQ0205624.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            58059..58997
                     /locus_tag="ACG8PO_000266"
     CDS             58059..58997
                     /locus_tag="ACG8PO_000266"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFQ0205625.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            59025..59405
                     /gene="rplQ"
                     /locus_tag="ACG8PO_000267"
     CDS             59025..59405
                     /gene="rplQ"
                     /locus_tag="ACG8PO_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFQ0205626.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            59677..60516
                     /locus_tag="ACG8PO_000268"
     CDS             59677..60516
                     /locus_tag="ACG8PO_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HFQ0205627.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMVFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            60492..61361
                     /locus_tag="ACG8PO_000269"
     CDS             60492..61361
                     /locus_tag="ACG8PO_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HFQ0205628.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            61351..62148
                     /locus_tag="ACG8PO_000270"
     CDS             61351..62148
                     /locus_tag="ACG8PO_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HFQ0205629.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            62351..63097
                     /gene="truA"
                     /locus_tag="ACG8PO_000271"
     CDS             62351..63097
                     /gene="truA"
                     /locus_tag="ACG8PO_000271"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFQ0205630.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKIRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            complement(63552..>66172)
                     /locus_tag="ACG8PO_000272"
     CDS             complement(63552..>66172)
                     /locus_tag="ACG8PO_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HFQ0205631.1"
                     /translation="ASFLIPFLILALVYLSIGIYPGSSRSILASDAFSQFSNFHASFR
                     NVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFPNQMMPDALYVLTLLKVG
                     CAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIMWLDAFFYLPLVILGIHR
                     VMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLLTNWSEYKKTIVPYGITS
                     LLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDLIMKNMIGVYDTTKYGSI
                     PFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASFYLVPLNLFWHGMHAPNM
                     FLFRYAYLFSFLVLLLAGKGWEKIEQDDRGLLLGIILVVAALFAVAWGIKPEGSYTYV
                     TMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMSAEALVNTNSMIHGILDD
                     WNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSANDSINYGYSGISLFSSI
                     RNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYNISKNESTFSKYGFRQAG
                     QSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNLINTLSGLNEQYFTFYTP
                     TLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQAYLSLFPTNFAQLESSS
                     ATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGTKEISFMEPKVVGLDVVA
                     YQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYDKGWKAKIDGKPVKVSNF
                     KDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFILYVWYLDRNRPFYGTIDP
                     AIEEQPRNTHRRRRRK"
BASE COUNT        20994 a        11898 c        14030 g        19250 t
ORIGIN      
        1 ccactaaacg gtgttctcct gccaactcct cggtcacaag tcacaatatt caacaaacat
       61 gagaagctgt tttttgaaat tgttccttgt gactcggagc taaacggcag gctcacaacc
      121 tcttggtaaa cggtgttcaa tcagctgacc ctcggtatta gctcaaaaaa cggcaaaata
      181 tgaggaacta ttttttccgt ttttttctct aatactcagg tcaaaacgct gatttcacaa
      241 cctctgattc acggtgttcc agaagtagct tctacggaaa taagccgaaa tttcacaaaa
      301 atttgagaag caattttcgt aaattccttc ttatttcttg aagctgacca cttctgtcac
      361 aacctcttat tttgattaga acttgcgaaa ataaaccggt taaagtgtgt ctagcgcaag
      421 agatttgcta taatagacga taagggtaaa atgtaacgag aaggggttga aaaatgtgta
      481 tcaaattgta acagattctt gttgtgatct gccataccat gtattagcaa aagaaaaagt
      541 tgatttttta tcaatgaatc tccaattgaa tgatcaagag ttagtagatg acctcggaaa
      601 gacattcaat tatgaccgct acatgaatca gctgaaaaat ggtgcgatgc ctacgacatc
      661 acaaatcaat gttggccgat atttagaatt tttccgttct tatgcggaaa aacagattcc
      721 tgttttatat gtggcttttt ccagtggatt aagcggctct tacagtagtg cgcttcaagc
      781 ggttgaattg ctgaaggaag aatatgaaga ccctgagatc tatgtttttg atacaaaagc
      841 tgcaagtatt ggggaagggc ttttagtatt ggaagctgta agattgaaaa aagaaggtaa
      901 aagcatcgaa gaaaccctag agtggctgga aagtaattat ttgaaagtcc attcatgggt
      961 aacagtagac gatctgaaat atctagaacg aggcggtagg atttccaaag caagtgctac
     1021 tcttggcagt ctattgagtg taaagcctat tattacggtg gataaagaag gaaggctgac
     1081 gaatgttgac aaagttcgag gaagaaacaa gtcgatccag acaatcgtca atgagacggt
     1141 aaaaacaatc gttgcaccac ttgatcaaat cgtttatgtg gcttacgctg gggatctaga
     1201 gagcgcggag aaggcaaaac ggcttttgga agagcagata aagatgaaag atgtgaagct
     1261 ttatccctta ggaccaacga tcgccagtca cacaggctat ggctgtatcg cgattttttc
     1321 gatgggaatc agtcgataaa agcagtaact cattctgtaa aaaagcccag aaattgattg
     1381 taacaaaaac agcgactctt aaaagataga tgagcatcct atctcttaga ggtcgctgtt
     1441 tttttatttt ttaagctaaa taggccatta tgcccataac tcgttcaatt gtttttgaat
     1501 atctgcattt tccaaaaatt catcatatgt cgtatcagct cgatcgatca cacctttatc
     1561 cgaaacggca atgatgcggt ttgccaatgt ttggataaat tgatggtcat gtgacgcaaa
     1621 taagattgag ccagtaaatg ccatcaagcc gtcattcaat gctgtgattg attctaagtc
     1681 taaatggttc gttggatcat ctaatacaag tacgttagct ttcgaaagca tcatttttga
     1741 aagcatgcaa cggactttct ctccaccaga taaaacattc actggtttca atacttcttc
     1801 accagaaaac aacattcttc ctaagaagct tcttaagaag gtgttatcat cttcttcttt
     1861 acttgcatat tgacgcaacc agtctaaaat cgttagatca gtatcgaatt ctttcgttgt
     1921 atcctttggt aaataagaat tacttgttgt cacaccccaa cgtactgttc cagtatctgg
     1981 cgtaatttca cccatgataa ctttgaatag cgttgttgtt gttatatcat tttctgcgac
     2041 gaaagcgacc ttgtcttctt tgctcaaatt aaaagatata tcatctaaaa ttttctttcc
     2101 atcaatcgtt acacttacat tttctacctg caataagtcg tttccaatct cacgttcagg
     2161 tttgaatcca acgaatggat aacgtctaga agaaggttgg atatcgtcta aagtaatttt
     2221 ttcaagcatt tttttacgtg aagttgcttg ttttgatttt gatgcattcg cactaaagcg
     2281 ggcaataaat tcttggagtt ctttgatttg ttcttctttt ttggcatttg cgtttgcttg
     2341 aagtttagcc gctagttgac tagattccaa ccaaaaatca taatttccta cataaagttt
     2401 gattttacca aaatctagat ccgccatatg tgtacatact ttgttcaaaa agtgacggtc
     2461 atgggataca acgatcacag tattttcaaa attgatcaaa aattcttcca gccaattgat
     2521 cgattggata tccagaccgt ttgttggttc gtctagcaaa agaacatcag gtttaccaaa
     2581 tagtgtttgt gctaataaca cttttacctt ttgtccacca gttaattcac tcatttgctg
     2641 atggtgcaat tcttcaggga tgttcagacc ttgcagtaaa acagctgctt ctggttcagc
     2701 ttcccatcca ttaagctcag caaattctcc ttctaattca gctgctcgaa taccatcttc
     2761 gtcagtgaaa tcctctttca tgtaaatcgc atctttttct ttcagcactt catacagacg
     2821 cttgtgtccc atgatgactg tttcaagtac agtatagtct tcatagtcgt aatggttctg
     2881 tttcaatacg gccatccgtt catctggtcc catagtaacg gaacctgtgg aaggctgcag
     2941 atcccctgat aacactttaa ggaacgttga tttacctgca ccatttgcac caatcaagcc
     3001 atagcagttt ccgggggtaa atttaatatt cacatcatca aatagtttgc ggtctgaaaa
     3061 gtgcaaactc acatcattta cagtaatcaa tttttttcct cacttttatc attattagtt
     3121 ttgacaagtc ttatagaatt atatagggga aaggtaaaag attcaaggca atcgaacatt
     3181 ctttgtgttt cacgtgaaac agaatgaaaa aaataaaaat aaaagcggag cgaaaatagc
     3241 tactatagga tttattagtt tgcaacagca taatttttga cgttatttat aagagggagt
     3301 actataaaac tgtaaaagaa atgaaagaag gattgaaaat ggatgaaaaa agaaccatcg
     3361 actggggctc attgattttg ggaattttat ttgtacttgt ctcattgtta tcatttcaag
     3421 accctgtcgg aaatttagtt gcaattgtag tagtctttgc gatttttgcc tttattaaag
     3481 ggatatttga actgtttgta cggaacagat taaaagagct aacaggatat aaaggaaaga
     3541 tgcccttaat aatcgggatc atcgatattc tagttggcgt ctttttctta ttcaatattg
     3601 gtgcaggagt cgcagcattg ccttttgttt ttgcagtatg gtttattgct gattcagttt
     3661 tagcgttatt tactgctgac ttggcaagag gagtaagtga aggatattat tggtttacga
     3721 tcatcgtcaa tattttagga attttattag gaattttctt attgtttaat ccaatttctt
     3781 ctgcgttgac attaagcttt ttagttggat tctattttat gttatttggt atcacacata
     3841 tcgtctatgc atttagataa gtaaaatcaa acaaataaaa aagccggctt ccgattacag
     3901 ttttactgtt gtaattggaa gccggctttt ttatcatgga caaatatgca cgaggtgatt
     3961 tttaggtgtc gagacaaatt tatgcaaaaa tgtgagaagc tatatacaca aattttatct
     4021 taatactcga agcatagcgc agttgtcaca acctcttttc atataattca ttcaatactg
     4081 gcaaatcatg gacttcatat ttttctttga atgcttggat ttcttcttca gaatattttc
     4141 ttggatctaa atgcagatct tctactggca atgggcgctt gttattgatc ttcacatcga
     4201 ttactacagg acgatctgat tttgcagctg catcaaaggc aaatcgaagt tgcgagtatt
     4261 ctgtgactgt aaagctattt gccccgagcg cttctccaac tttaccgaaa tctgcatctt
     4321 ctaaaaatac accaaatttc ttttgttccg tatcttcttg ttctgcttcg atgaaaccaa
     4381 aagaatcatt cgaaaagaca acattaataa ttggaagctg atatttgact tgtgtaatga
     4441 tatcttgcat gaccatagca aacccgccat caccacttaa ggtaaatact tgtttttctg
     4501 ggtagcttag ttgtgcggca attcctcctg gcacaccatt acccatagtt gcgaaccatc
     4561 cagatgtcgt atgtttttgt ttaccgttca tattcaataa acgaatagaa tggatagtcg
     4621 tgttaccgac atctgtgacg aaaatcgcat cgtcttcagc aatacgattg atttctttat
     4681 agacagcctc tggacgaatc ggttcttcct ctcgatcttc aaaagatttc aaccaattta
     4741 cccatttttc tttattttct tgatttgcta ggtaccagct atcagcaggg cgagcatccc
     4801 ctaactcgac aagtctttgc aaagctgtgt ttgcatcacc gaggatcgac aaagatgttt
     4861 catggcgtcg tccaaatttt gacgcatcga tatcgatttg gataaattca gcagttggat
     4921 taaaaaatgc acgtccaaat gggaagtcgc taccgacaaa taagatcaga tcggcttctg
     4981 ccaatgcttc atttccgggt ttagtagcga cacgtccagc aaaaccaaga aagttttcat
     5041 aatcatcagg aataattcct tttgctagaa cggaagcgac aacaggaact gaaaaatgtt
     5101 ctgagaattc tttgatttgc ggaaatccgt ttcgcgttcc ttgaccaaca taaagaactg
     5161 gtttttttgc tttttcaaga taaggcaaag cagctagcaa atctttttct tctggtaata
     5221 tcacacttgt tttgtgagtg tgagcggtgg agaaaggttg ttcgtcaatt tcttcaaaac
     5281 caagatcaac agggatagtc acgatagcca cgcctttatg ctcataggcg gctttaatgg
     5341 cttcatctac tacatgcgga agactttgtg gtgtcatgac tgtacgattg tatacgctaa
     5401 catctgcgaa aataggattt tcatttagtt cctggaaaga gttatagttc atggatgtag
     5461 aagcaacttg tcctaagaac gctactagag gcacatggtc catctgtgca tcatacaaac
     5521 cattgatcaa atgtgtcgca ccaggtcctg cagagccgaa gacagcgccg actttccccg
     5581 ttaattttgc atctgcagcg gctgcgagtg cgccgacttc ttcatgacga acttgaatgt
     5641 agttgatttc ggttttttct tgatatagcg catccataat tgaattgaaa gatcctccgg
     5701 gaatgccgta aatatggtcg ataccccagc tttcaagaac ttttaccatt gcgattcctg
     5761 cattgatttt tgacatggtg tttcactcct ttagcaatga tgatacataa attttttgta
     5821 tctagagtat agaacttttt atcgaaaatc ccaactaatt cgtcttttca caaaaatgta
     5881 gtcatgtttt tgggaatatg ctagagtagt acatagatag attcaagaga ggggaatggc
     5941 atggttgaat ggctattgag acttgaacct tggcagcaag cacttgctgg gacagctttt
     6001 acttatttta tgacagctct tggtgccggg ttggtttttt tctttaaaga aatcaaaaaa
     6061 gaagtgctaa acttgatgtt aggatttgct tctggtgtca tgattgctgc aagtttttgg
     6121 tctttgttag atccggctat tacaaaagca gaagaaaatg gagatattgc ttggctagtt
     6181 gtaagtattg ggtttggttt aggcggtgtg tttttatata tggcagataa aacactgccc
     6241 catatgcatt ttggaccaca acatgaagca gaaggattgc ctacccattt aaaacggacg
     6301 atcttgcttg ttttttctat tacattgcat aacattcctg aaggtttagc cgtgggggta
     6361 gcattcggtg cagcagcaac tgcggataat ccgacagccg ctatattagc cgctgtttcg
     6421 gtggcgctag gtattggtat ccaaaacttc ccagaaggag cagctgtatc aatcccgttg
     6481 cgacaagaag gtctgagtcg taaaaaggcc tttgtttacg gacaagcttc tggaatcgtt
     6541 gaaccaattg ctggagtgat tggagcgcta ctagtaacga aagtggagct gctgcttcct
     6601 tatgcacttg cttttgccgc aggagcaatg atttatgtcg tggtggaaga attgatccct
     6661 gaagcacagc agacattgtc cagcaaacgt cattttgcag tttttggcgt catgagtgga
     6721 tttatcatta tgatgatttt ggatgtggca ttaggataaa taaaaaaaat atatatgata
     6781 tatcaaatag ggccgcgaac ttatatcgcg accctatttt tttagtaagt tatatcataa
     6841 tgaagtgaat ggtatcatac attaataata gttttttcta tgtgtatttc ctttgatcca
     6901 gcgaacaagc cagatgataa gtgcgattgg gattagaact ttgaccgcaa accataaaag
     6961 cccgcttagg acactgacaa aaagactgat gacgaagcta gctgcaatta gcactaaaag
     7021 aaccatgaag atatttaaga acatattttt ttgacgcata caaaaccctc ctcttgttca
     7081 ttctttacta tagaaaaagt ataacaggga ttgtttaaaa acacctccaa ctgaagactg
     7141 atttttactt catctactta aatgatcaaa caattgcttt ttctctggtt tatcatctaa
     7201 aatagagcgg aaaaggagga gaatatcatg cctaaaaatc gtcttgaagc atttacagat
     7261 gctgtaatcg cgattatcat gactatttta gttttagaac ttcatgaacc taacggagac
     7321 acgtgggagg cgctcgctgc attaggttac cgatttttta tctatattgt cagttttatc
     7381 attttagcga tttattggaa caatcatcat catttatttc aagttgtaca taagataaat
     7441 ggacgggtct tatgggcgaa cagtttcttt gtttttgcgc tttcgctttt tccgtttgca
     7501 acttcatgga tcagtgagta catcaatagc ctggctccag aaatgactta tggttttgtt
     7561 accttgaccg ctaatattgc gtattatatc ttggcaaaag aacttgtcaa agcaaataaa
     7621 ggccacgaga aagcattgaa ggcgatgttg aataattaca agaaatcgta tctatcgatt
     7681 ggtttgaatg tgttaggaat tgtcttaggg tatttagtag cgccagtagc cgtgctgatc
     7741 gcaaacatcg tgattctagc tgcttggatc atcccaagtc gtaccatcga atcacaatat
     7801 cggtaaaatg atccgtgttt aaaatttaag agaaaaatat agaaaaaata aataaacgat
     7861 caaatatccg agtgatacta gtattcaaag agggatgcag atggatcaaa aaagaattca
     7921 tgtaggactg attactagtt ttgtcacgat gcttatttat ttggatcaag gtcatttgac
     7981 ttggacagta gttggtgtcg taggattttt gacattattc acagcaataa aaaaataaac
     8041 atctttatgt aatctgatgt tctatctatt taagaaaagc atgtttacag cagtatgatt
     8101 ctgttgtaaa cgtgttttta tttgtggaaa atttaatttt tagaaaagtg tatcgaaaaa
     8161 tagatagtta gtttttccac agtggataac ttcgtaagag ataaaaataa aaaattagtc
     8221 aaagaagggc gtatagattt cttttgatag gagatataca acagaaatga ataaatatta
     8281 acgtgttttc aaacagtata aaacggtgtg gtttttggaa taaaaaaata acatatctca
     8341 ttttacactt ttgttatttt atacttaaaa aataacgaca cagaaaatac cgtgtctcag
     8401 atttctctac tacttctaaa aactactgtt tttttccttt gaaatgcctt caaaaagtag
     8461 gatcaggcag aatatactgg cagaatcaag gagaagggag cctaaataag gagaaaaacc
     8521 atagaaaata taaagtaatt acttataaaa caagcttttt atagaaaaat aatatacctg
     8581 agtagggtat aaattaagac ggaattcctt tctagattga gatattgact aggaagaatg
     8641 ttaagatttt aagcctttaa gctaaaaaaa aacccttttt gttaaataag gggaatatag
     8701 cctgtcagtt tcaaactgca agtcgtcctg ctgcaataat atagtagatt agagatgaaa
     8761 atagattttt ttaaaccaat gactgaataa aaagaccgtc atagatacac attggaatgt
     8821 gtctattacg gtcttttttt acgaacgatt ttattgaaga cggacagttc gggtcaaaaa
     8881 taaaaaagct acgatcagaa ataagaataa aatgtatccc aaaataatgg ctagacggtc
     8941 taacttattt gtcgatagat gtttgtttag aagttgatag gcccataaac ctatcagtcc
     9001 tcctgttgta ttagttatca aatcagtgat gtccgctacg ccaataccga ataggaattg
     9061 aatcaattct gccaagaagc ttacaaggaa aataatccct aattttttag aaaaagaaag
     9121 tctcttgaaa ttgacattca aaagcacccc aaaaggaaaa aagacaatca agttataagt
     9181 ggtctcacgt aataccgtcg tattttcaaa agaaaaagta gaaaaaggga cgagattgac
     9241 attgctgtaa gaataagtga gaaattgtgg aagattaaat gatagcttga ataaaatcaa
     9301 ccataataga aagaataaat aaatccctaa taaaaatttt gatacatacc gcatagacat
     9361 ctcctccatt taaaaagtat accatcgaaa accgctgaaa aatcataaaa aagtatgaag
     9421 tttaaggacc aacgtagtta tcgtacgaaa attagaagct tttttttgtt gggaatccgg
     9481 tttctttaac aaagctaaga tagcgagttg cttggaaaag ttgctatctt ttttctgtca
     9541 aaacgactcg gaatgttcgt tttttgttct aatttctcca agttgtttac tttttgatac
     9601 taagtttatg aaaaatcaag aaatagttgt tatactaggt aatgctgaca aacgggattg
     9661 gcatttcatc ttggttcagc tttttttcca attactagga aggagggata gattgttcaa
     9721 gacttatttt cgtttcaata aaatacaaga taatcctttt cgtatcatga atctgatgct
     9781 acttatcttt attgtattat ggttaggtta cataaatcat gattttttga tttcaagttt
     9841 ttcatcatta gggatcttta cttttttcta ttatcaaaac ataccgatga aatatttgct
     9901 gaaacgtatg tttctgatcg gttgcggact gttggttgct ttcgttttgg ggatcttatc
     9961 gacttatgtt ttatggcttg aaccatttgc cgtagctttg gttgcttttt ccagcagatt
    10021 cgtgttgcga ttattccata tttctaaacc aggtggcttg ttttttgcga tgctttctgc
    10081 catgggaacg agcatgcagc tgccaatcgc tcaattaccg atcgtctcac tttatttttt
    10141 tatgggtgtc gtttttgccc tgattgcggc agtgattaca aaattactgg attcacggcc
    10201 tgagcagaca attgaaaaag caactttaaa agaaagattt cacgaagagc ctttagtgat
    10261 cattgattct gttttctaca gcgctgcctt atttctttcc gtttatgtca gtcacggctt
    10321 gaatctgcat aatccttatt ggttgacctt gtcatgtgca tcgattttgc tagcagaaaa
    10381 cttggatgcg atgaagcatc gacaagtcca atatctgatt ggatcgatgg gtgggttatg
    10441 tgtgtcggca ttcttgtcat ttgtgccgtt tacgcagttg cagaccattt ttctgatcac
    10501 ttttctatat ggaatcgcac aatttctagt tgctagaaat tatgcagttg ctaatatttt
    10561 tcttaatccg atggcgttga tgctatctac acttattcgt ggagcatact tgatttcttt
    10621 gatcgaatat cgttttttag ggatcgtcat aggaagtttc atcggtttag gtgttgcgtg
    10681 ggtaatgacg gttggtttgc aacattattt aacggttgtt cgtcaaaaat tagaatagaa
    10741 ggacaaatat atgtgccaaa agaggttatg acaagatttt tgtcacaacc tctaattcaa
    10801 ggtgttcaaa agccagattc tcagcattaa gtcaaattca cacaaaaatg tggaaaggaa
    10861 ctttcaaaaa aattttatat ttattctatc aaaaaatttt gtttatgatg gtaactgaat
    10921 aaactttcct gagatggtca atgctttcct tgaaagtctt acagatattc tttcttcata
    10981 gaaagtttaa gttttatagt tagtgtacaa taaaagtatg tttataccat atggtgtccg
    11041 gataatcagt catttaaaca atgaggttca aagatatgaa aaggggaaat ttgatgaaga
    11101 aaataagcaa tgaaatccaa attctggcag aaactttaac tcttcccaaa gtcgttatag
    11161 agcaaatgat tgtacaagca aaaaacatcc ttgatgaggt gtttgagcaa tatagtcatg
    11221 aactaatgga tcctaataaa gcaaaggcga ctgcaaagaa aatagacaaa gtctatgaag
    11281 aggaaccttt tgcagcatta gctatttatc tatatgcagc ttctcaaagt tggaaaaaaa
    11341 tttatcaacc attgaagatt ccaagaacta tctatttggc aacaatgaat gcttttacta
    11401 gatttattca tgaacatttc caagtaacag gaagctatca ctttgaccgt ggtttttgga
    11461 tttggcatta cactagtggc ttgatctttc ggattggtga attggagtac gaaatgacat
    11521 attttccgaa agggtacacg gatctaagat tagaaggaaa gcagtgcctt tctcttcata
    11581 ttccttccga tgcagattta tctcagaata agataagaga tagttacgaa agtgccattc
    11641 gtttttttga actttatttt cctgattacc attaccaagt gatgtacaca gatacttggt
    11701 tactatcacc caatcttact aaatggttga aaaaagagtc aaaaatctgt ctttttgcag
    11761 ccgattatcg attattatca gttgatgaac aggacgatag tggagtccct tggatctttg
    11821 gacgagtaga tgcccaaata catgattatc cagaaagtac gagtttacag cgacaagcaa
    11881 aagagcaatt gcttgcaggc gagcacattg gctctggctt aggtattctt acttcataag
    11941 tttagtactt cgaaatagtt taaaagagcg tgagacaaaa gtaaagaaca cttttgtctc
    12001 acgctctaaa cacgtataaa cggcggaagc agaagcaact ccttcggaaa taagctgaaa
    12061 ttcacaaaaa tttgaaaagc aattttcgtg aatttcttct tgtttcttgg agttaaacgc
    12121 ttctgtccca gcctcttttt tagtgttcta tcgaacacac agtacgttca ataggtaaac
    12181 tagctataac aatagatatt aaagttaacg gacaagaggg ccttttttag gtagctaaaa
    12241 cgtaataatg aaaatttatt tccagagata ggagtcaatc agctagtctg atcgactgtt
    12301 ttaacaagtt gactttcatt ttaacttttg ataccattta aaacacaaat cgtaattgtt
    12361 tcgatttgtg ttttttagca tgactacaat caaagaagaa agtgggtcgt ccatatggaa
    12421 aaaacagata tctcaagtgc ataccgccgc ttgaaaagcc caaatatcaa aacacgaaaa
    12481 cgtgcgctga agatcatcaa agatgtgaaa cggaatagtg ggaaaaggta agaagaagga
    12541 aacttaatta aaaaaatagc cgaaaaacgt ttttcggcta ttttcaataa tcggtttttg
    12601 tgtaaggttt ccgaaagaac tgctgttgat tttccatttt taacatatga taaattggta
    12661 gaatctttct tttcaaattg ataccattcc ctaattccag ttatatagtt aactacagaa
    12721 ccacaaagca cgtacgttta taaaagtatt atctgtagtt atttttcaat tccctttcaa
    12781 cctcttattt tgaccagtag tattgatcac tttacctttt aacacctttg ttcgtttttg
    12841 aaacattttt agtcaagaat ggaaacgtaa ctacataatc aaaggttttg cgaaatattt
    12901 catgaaatat ttcatgtaga agcgagcaat acggttgcaa ataaccatta tcgtttaata
    12961 agcagttacg aaagctccca atatctctaa cgttcagctc actccatttc ttttcttcaa
    13021 aaaaatcagt gattcgtatc tctttctatt tgtcgaaaaa gttaacttta aaaagtaacg
    13081 ccgaaaaaat atgcaaaaaa ataaaaaagc agttactatt gctagaataa catcaaggaa
    13141 taataagttt tggttttcag ataatagtcg ataagttgtg tattggaatt ttagatacag
    13201 taataaagta tttttcatga aattttgtga gaatcactat tacaagtatc tttaataaaa
    13261 ttaatggtta aggaggaact taaagtagaa tgtaaatgaa tagttgtagt cagtcgattg
    13321 tgatactaga ctcaaagttg caggaataag tttttcaatt tgtgattgga agataaaaaa
    13381 tgaaaaagaa gggctttttt ttatactttg ttgattgtag tgtttatttt ccctctgttt
    13441 agccaagtaa cattttctat gactcaacca gtttcaagtg aggaacatga taaccttttt
    13501 cgtgctacag aagagtttaa gaaatatcag gaaaatattg ttccggagac tccattagct
    13561 agtgagccaa ttactgatat tgatggaaac caaataggat ggtatataca gtacgaagtt
    13621 atggtggtaa ataatgactc aatagtaaaa aatacagtag gcttagagct aacaccgatt
    13681 gaaaaggtga gttcattgtt aacgtatatt tatactgatg aaagtgacga gactgagatc
    13741 attttaacgg attactcaaa aattgctact gataaaaagt atatattgtg gatgtagaca
    13801 ctagtgaaat actttatgat atagaaattg atgaaaattt actagatgta gcacaagaaa
    13861 tcgatcatca aaagcaggaa gctattcgat atgctggtag aaaagggaaa agaaaatatt
    13921 tattacgtga atgtagcaaa agttagagaa gatgaaaatg agtggaaaga attcaagagt
    13981 cgctactcaa ttaactcgac tccgacattt acagtttaca gagaaggatc gatagaaaaa
    14041 actgtttttt ggactaaaga aagtggaatg tctttagctg aagtagaaga gtttttggat
    14101 tatgttagta tgcaacagta agggagttct tattatgaaa aattttgaca catattatgt
    14161 tattgttttt tcgatatctt tgctgttaat attactaact ttaatgcagt ataaagagta
    14221 tccagagtat tttattttaa cttttgtata tttagtaagt agctacacac ttctatataa
    14281 attcagaagt tcttctagag tttctaaagt ttgtttctta attagcttat tgattctata
    14341 atatttttga tttatatagg aaattaaaaa tattatagaa tcaacactca gagaacgggt
    14401 tggtttttaa tagatttaat aagtactgat aatgaaattt ctcctggttg ggaaaacgaa
    14461 gttggttacc ctgtagaaga attttttcaa gattactttg cggagtatgc cgataggata
    14521 atgagagatt acgtaacaga taaaactcaa gaagtcatca atcaatccca gtcattaaat
    14581 gaattatctg ataaggcgaa agaagaagca gttgccatta tttcttcagg gattagtgac
    14641 tatattgata tgagtggtga aatcaattat ggcgataaag acaaaattgt acgcatgata
    14701 aaagctttta tcaaaacttt tgatgtgaag gcagatctta agtttaatga tacttaccta
    14761 gtaggaaaat taattaatat ccttgggaca gagaaagata cggaaacgtt tatttcctct
    14821 ttgtctatgt tgtttacaga taagcatttt tcaactcagc ttggtggaga ggaactagtg
    14881 gaaatctttt aaccattacg cccagcatta ataaaagtat atcaaaaaag tactcctgat
    14941 gatttttaag attggtttga gaaataatgt gaaaacacta tttttaatgt taattataga
    15001 tagagatgaa cgcgtcatat ggttaaaaga atgaaaaagt caaagataca ctgctatatt
    15061 tcactaagga tatagcagtg tatctttcat gtcgtttcac tggcagtttt ttataaaagg
    15121 aggtgtgcat ttcagttgct tttctttggt agttagttga atcgtttaag aattaacgga
    15181 tgtggcagac aggtgctctg tccaaaaacc agtaaaatct ttgatggttc catctttatt
    15241 tagctgtgcc tgttcaaata ctgttttgtt tttaccgaat ggatcaattg caataattgt
    15301 tccgatcccc gcacgtttgg cagcaagtaa tcctgagtaa gcatcttcga taacaagaca
    15361 atcagccgga gctaagctca gtttgtctgc cgctttaaga aaaatatccg gttgcggttt
    15421 cccagggaaa gagccgtcgt catagacaat cttgtcccag tcaaaccagc gggctaaatc
    15481 gaaaatatca aaataaaaag caacattctc tttgacagta gccgtggcaa tcgttaatgg
    15541 aatctgttgg tcagtcagct ggtcaagagt cgcttcaagc ccttttgtca actgaagctc
    15601 gttttcattt tgcaagcaaa gcatacgata atagctttct ttttcttctg acaatacggc
    15661 gatctcagag gcggtcaaat gctctgaaat gaaatgtttc aaaatctccg tattcgtccg
    15721 accatgaata ttttttaaaa tatcctcgtc tgataaacca tcttttgtat gatcatgtat
    15781 catatgcagc caagcagctt catgcagatg agagtccaaa aacattgttc cgttgaaatc
    15841 aaatataatc cctttcatag ttacgctcct atagtggtat tttcggttga atcagcaatt
    15901 tgcagatcga catattgtgc atagtgctct tgaacctccc gcgagatctg gcgatccgta
    15961 atgagcagat cgaaatcatg caaattataa aacacataaa aatcatctcg attgaatttt
    16021 ttattatcaa cgagtagata tttcttacgg gaattattta gagcgatctg ctgtgcttct
    16081 ccttcttcgg tactgtaagt agagatcgct tcgttgtgaa cgccatttga actgataaat
    16141 gcttttgtaa actttagtct tcttaaattg tcgttggtga tcggtccgac aaatgcgccg
    16201 gtatttttcc ggaaatcacc gccaatcagt agaatttctg gtccatctgc ttgagcaaga
    16261 atatcaaaaa ctggataatt atttgtaatg atccgaattt tcttgttcag taaatgcgtc
    16321 gccaatagtt cgattgttgt acctggtcct aagaaaatcg tttctccctc gtgaatcaag
    16381 gaggctgctg tggcagcaat ttcttttttt tcttgaatct gcagagtggc tttttcagtg
    16441 tgagaaagtt cttgatctaa agtgtaggaa agactttgag cccctccatg gacacgcagc
    16501 agcttgcccg atttttctaa ttcatctaaa tctctgcgaa cggtcatatc agaaacatct
    16561 aattgattga tgatttcatt aacggtaagg attccttttt tattgaccat ttctacgata
    16621 gtaagcaatc tttctttttt caacattgtc ttcacctcta atttaaggat aatctatcat
    16681 ataaacggac aacagtcaat ctaattcgaa caaaagtaaa catttagggt ggtttttatg
    16741 tttgttacaa tagattttat tgctgttttc tggtttttat gattatttgt gtttgttttt
    16801 gttgacaaat gttttggatg cgtttacaat gcaatcagtt agaaagagcg aaaactgatt
    16861 atagatttga ggagagatgt acatgcttaa attaccagaa gattttattt ttggcggagc
    16921 cacagcagcc tatcaggttg aaggagccac taaggaaggt ggcaaaggcg ctgttgcttg
    16981 ggatgacttt ttagaggaac aagggcgatt cagtcccgat ccggcaagtg atttttatca
    17041 tcaatatgca aaggacattg aattgtgtga acgttttggt gtaaatggtc taagattatc
    17101 cattgcttgg agccgcattt ttcctgatgg agctgggaaa ccaaatccag aaggaatcgc
    17161 tttttatcat cgtgtgttcg aagaatgtaa gaaaagaaac gtaacgccgt ttgtgacgtt
    17221 gcatcatttt gataccccta aacgtttgtt tgaccatgga gattttttga atcgagaaac
    17281 aatagaagca tttgtttcct atgccatttt ttgttttcat gaattcaaag aagtcaaggt
    17341 gtggagtacc tttaatgaaa tctatccagt tgcaaccaat caatatttgt taggtgtttt
    17401 cccaccagga atcaaatatg attttacaaa gatcgtcgcc tgtcttcata atatgatggt
    17461 tgctcatgcc cgtgtagtga actatttcaa agaaaacgaa cttccgggtg aaattggtgt
    17521 agttcactca ttagagacga aatatgcggc aacagacgcg ccggaagata agcatgctgc
    17581 ctttttagac gatgcattgt cgatccgttt cttattagat gctacctatt taggctatta
    17641 ttcaactgaa accttgactg ctcttgatga gatatgtgaa gcaaatcagg ccagctatca
    17701 ttttcctgaa gaagatttcg ttgaattgaa aaaagcatcc acgcgtaatg actacttagg
    17761 gatcaatcac taccaatgcc attttgtcaa agcttacgat ggagaaaatg caatccatca
    17821 taacggtacg ggtgaaaaag ggacctctgt ttataaagtg aagggaatcg gagaacgtat
    17881 ttataaagaa ggaattcctc gaactgattg ggactggctg atttatccag aaggtctata
    17941 tgacttgttg ctgcgaatta aaagcgatta cccacattac aacaaaatct atatcactga
    18001 gaacgggatg gggtataaag atcagtttga agatggcatt attatggatc agccgagaat
    18061 cgactatttg agagtctatt tggaatcctt aagcaaggcg ataacagccg gggtaaatgt
    18121 gaaggggtat tttttgtggt cgcttatgga tcttttctca tggacaaatg ggtataacaa
    18181 aaggtatggc ttgttttatg ttgattttga aacccagaag cgttacccaa aagaatcagc
    18241 ttactggtac aagttggtca gtgaaaccaa aacgattatt taataagtga taatggagga
    18301 agaaaagatg aataaagagg aattacaaat gcttggtttt gagatcgttg catattcagg
    18361 cgacgctcgc agtacattat tgaaattatt gaaagaagta agacaaggaa actttgaaca
    18421 tgttgattcg gcgttgaaag aagccgacga aaatttaacc cttgcacata attcgcaaac
    18481 aaaaatttta gcggaagaag catctgggaa agagatggag atgggattca tttttatcca
    18541 tgggcaggat catttaatga ccactttatt gctgagagat ctgattcaag actttattgt
    18601 actgtacaga cagaatcaag gataggagaa gaacgctatg gatggaatca ttaaacaaat
    18661 cgaaaaaggg aaaccatttt ttgaaaaaat ctcacgtaat agttatttag gagccgttcg
    18721 tgacggattt ttaacagcga tgccagctat tttattttca agcattttca ttttgattgc
    18781 tgcggttcct gagatttttg gttaccaatg gcctgaatct gtctccacat ggctatggaa
    18841 agtctataat tattcgatgg gtgttgtagg gttactcgtt tcagcaacca ctgctcgctg
    18901 cttggctgat tcgatgaatc gtcgcttacc gaaaaacaaa gtaatcaaca ccacctctgt
    18961 gatgctggcc tctgtggtaa gtttcctgtt gttaagcgtc tctccgattg aaggtggatt
    19021 ttcaacggaa tatatgggga caaagggact actggcttcc tttgtcgctg catttttaac
    19081 agttaattta tataagtttt gtgtgctgaa agatattacc attaaaatgc ctaaagaagt
    19141 tcctggaacg atttcgcaaa ccttccgaga tatttttcct ttctcttttt cagtatttgc
    19201 ggccgtttta attgatttag gcattcgaca aatcttccaa atctcatttg cagaagcaat
    19261 cattacggtg ctgcagccgc tatttacagc agctgacggg tacttaggaa tcgctattat
    19321 ctggggagcg atggcgctgt tttggtttgt cggtgttcat ggaccgtcga tcgtggagcc
    19381 ggcaattgcg gcaattatct atgccaatgt ggaaaccaat ctgcaattgt tcaattccgg
    19441 tgaacaagct tctaaagtac tgacggtggg tttagggaat tttgtaggaa ctatgggagg
    19501 aactggggca acattggttg ttccttttat cttcctactc tttgctaagt ctaagcagtt
    19561 gaaggcagtt gggaaagctt cctttattcc agtaagtttc gctgtcaacg aaccattgct
    19621 atttgcggca cccatgattt tgaaccctta tttttttatt ccatttttgt taacacctgt
    19681 tgtaaacgtt tgcctgttca agttttttgt cgatgtattg ggcatgaaca gctttatgta
    19741 cgttttgcct tgggcaactc cagcaccgat cggtttggtt ctaggaacgg gcatgggtat
    19801 cttagctttt gttctagcag cggttctaat cgtcgttgac ttcctgatct atctaccatt
    19861 ttgcaaagca tacgatgctg tcttagtaga gaaagaagca caaaaagcag aggtgatcgc
    19921 aaccgaggaa ggtgcttctg ccgatgtacg tgaggaagta cctgtggcag cgagtcaagt
    19981 tatcagcgat gcaaataaag aaatcaatgt gttggtactt tgtgctggcg cagggacaag
    20041 tgcgatgtta gctaatgctt taaaaaaagg tgcatctgaa tcgaaagcac cgatcagtgc
    20101 aatggcgggt gcttatggtt ctcattacga aatgctgcag gattttgata tggtggtctt
    20161 agcaccgcaa gtcaattctt actatgatga tatcaaagaa gacaccgatc gcttaggaat
    20221 caagctggtt gcgacaaaag gagcagaata tatcggatta acgagagatc ctgaaaaagc
    20281 tgttcagttt attctgaacc aatttcaata aaggaggagc cataatgaaa atgggaattt
    20341 ggttgcgtaa agctgtttta tttgcagctg tgttaggggc tttgggggct gtttcaaaaa
    20401 ctgttgaagc atctgccagc gaatttattc gcggagcaga tatttcgatc ctgtcagata
    20461 tggaaaaatc tggtgcaaaa tattatgaag acgatatttc aaaagatgcc ttagagattt
    20521 taaaaaataa tggtgtcaac tatgttcgac tgcgtttgtg gcaggatcct tataatagta
    20581 atggagaaag ttatggagca gggacaaatg atctggaaac gacgattgac cttgcaaaac
    20641 gagcaaaaaa tctgggactt aaggtgctgc tggattttca ctacagtgat ttttgggttg
    20701 atcctgggaa acagaatctt cctaaagcat ggcaaggtct gacctttgaa gaaatgaata
    20761 cggcattgta cgactataca aagaacgttc tgtcggaaat gaagcagctg gacgtttatc
    20821 ctgatatggt tcagatcggc aatgaactga atagcggaat gctctggcct tatggcaagt
    20881 cttggggaga aggcggggga gaatttgatc gattggctgc ttttttgaag tctggtatcc
    20941 aagctgttcg agacactcaa cctaaaacaa ctccagtaat gctccactta gctgatggtg
    21001 gcgatacagg agcatttact tggtggtttg atgaaatcac gagtcgaggt gtgtcatttg
    21061 atttaattgg agtgtcgtat tatccgtatt ggcacggttc aatgaatgaa cttcaaacca
    21121 atatgaatgc gatcagccaa cggtatggta aagaagtgat cgtagttgaa acagcttatg
    21181 ggcatacaac agcgaatgcc gatactatgc caaatgcttt tggtgaagcc gaagcagctg
    21241 cggggggata tgaaccaaca ccgacaggac aagcagagta tttgctggat cttgccgatc
    21301 ggattcaggc agtaccgaat aatcgcgggg cgggcttctt ctattgggaa cctctttggt
    21361 acaacggaaa tgtgagttgg gcaacacaag caggtatgaa ttatctcagt gttcaatcca
    21421 ctatgggaaa cgaatgggac aaccaagcga tgtttgattt tgctggaaac gccttgccaa
    21481 gcttgcgtgc gtttaaacaa gcaggggcgc aaataaatct agttaaaaat gcaagtttcg
    21541 aaagcgatgg accaacgacc agtccttcca attggcaaac ctggtttcaa caaggaaccg
    21601 ctaagggtac gatcaaaacg gaagcgggca ctttttttgg ggactacaaa ctaacttttt
    21661 ggcaggatca agcatacgag gcatcagctt atcaaaatgt tagtggcata agcaaaggaa
    21721 cttacacatt gagtgcctat gtaatgtctg ggggagagtt tgatactaat cagttatata
    21781 tcaaaaatta tggtggagcg gaactaaata ctgcggttgt taaaacaagt gaatggacaa
    21841 aaattacaat caaagacatt ccaattacaa caggtgtttg tgaaattggg gtttatagca
    21901 aggcttcagg aaatgcttgg acgaacgttg attttgtttc attggtgaaa caatagactg
    21961 cggatcgttt cgattcaaga tcggaggttc ccttcagcca catcgttagc gtagaaggca
    22021 ggaatcgaac ctgcacctcg cctttgccaa aaataaccag aaacgatttc cagtcatttt
    22081 taccaaaacg agtaaagcac cagacttcat ctacactttg tattatgcta aagagagttc
    22141 aacgatttcg taagaagcaa tgaaaaaatt gcaaaaagtt gtgcagataa cataaagttt
    22201 gtttaagaaa gggtaacctt agaattccgt cgtgaatatg atttgagcac ttagatggat
    22261 agaaaaaagt tctcggaaaa cagttcgttt ttcgagaact tctttttatg ataagaatga
    22321 aacaaattaa cttatattat agtctctttt ggtagggaac catcttattt ttaatgaaaa
    22381 tcaagcattt tagctattga agaatgttga tttataggga ttccccctcc atattatgtt
    22441 ggctctttat tcccactcca ctgttgctgg tggtttagaa gtgatgtcat acacgatacg
    22501 gttgacgtgg gctacttcgt ttacgatacg tacagagatt ttttgcaaga cttcccatgg
    22561 gatacgggca aagtcagcgg tcattccgtc gattgaagtt acagcacgga taccgactgt
    22621 gtagtcgtaa gtacgtccgt cacccataac accgacagag cggatccctg gaagaacagt
    22681 gaaatattgc cagatatcac ggtcaagtcc agcagctgcg atttcttcac gcaagatagc
    22741 atcagattcg cgtacgattt ctagcttgtc ttcagtgatt tcgccaagaa cacgaatacc
    22801 aagaccagga ccagggaatg gctgacgcca aacgattgaa tcaggcatgc ctaactgagt
    22861 ccctaattca cggacttcgt ctttgaataa tgtgtttaat ggttcgatca attcaaattg
    22921 catatcttct ggcagtccac ctacattatg gtgagatttg atcgtctgtg ctgtttctgt
    22981 accagattcg ataacgtcag tatacaacgt tccttgtgct aggaaagaga cgccttcttc
    23041 accagcaagt tttgttgctt cgtcatcaaa aacatatacg aattcattcc caatgatttt
    23101 acgtttttgt tctgggtcag atacaccttt caacttgtct aagaagcgat ctcttgcatt
    23161 gactttgatg atgttcaagc cgaattttcc gcctagactt tccattactt gttccgcttc
    23221 gcctttgcgt agcagaccgt ggtcaacaaa gatagatgtc aattggtcac cgatcgcttt
    23281 ttgtaaaaga accccaacga cactagaatc aacgccacct gaaagaccaa gcaagacttt
    23341 tttgtcacca acttgttcac ggattttttc tacttgcata tcgataaagt tatccattgt
    23401 ccagttacct tcacagtgac aaacatcaaa ggcaaagtgg cgcagcaatt cgattccgta
    23461 ttcggaatga cgtacttctg catggaattg gataccataa aaattacgtg ttgtgtcttg
    23521 gatagaagca atcggacaat ctttgcttgt tccgactgtt tcaaagcctt caggaacttt
    23581 agttaccaag tcaccatggc tcatccatac tgtttgttgt tttggtgttt ctttaaacaa
    23641 gacagcttga tcgcttgtta cttctaacaa ggctttccca tattcgcggt ttttagcagg
    23701 ctctacggaa ccgccaagtt tgtacgtgat caattgcata ccatagcaaa tccctaatac
    23761 aggaatacct aaattgaaga tctcaggatc gatcccaaat gcattctcat catatacact
    23821 gtttggtccg ccagaaagaa tgattccctt tggattcatc gcttttactt cttctgcagt
    23881 aatgcggtgg ctcaataatt cggaaaagac tccaagatca cgaatacgtc ttgtgatcaa
    23941 ttggttatac tgactaccgt agtctaatac gataatcttt tcaacgttcg tcatatcggc
    24001 aacgtttgtc aaatttatta cccctatccc tttaaatttt atctcaaaat agtcaaacta
    24061 ttttaaaatg ctaatatttt cgcatgaaaa tcgaatcgat gatatgattt tcagttttat
    24121 ggagaattat atcggctctt cctcgtgtag gcaagatata ttcctgtaaa tttttcaaat
    24181 tcacattttt ccatacatct tttgccattt taaaggcttc ttcacgatct ccgatagcaa
    24241 aacgataata ataattctct ggattttgga aagccgtatc cagtaacgca ccaaaacgat
    24301 ccaaatacca tttttcgatc agcacgggat cggcatcgac atagacagaa aaatcaaaaa
    24361 agtcgctgac gtagatttgc tgattcgcag gtaattgaag cgtattgatc ccctcaacga
    24421 tcaagatatc aggctgctgg atcaattcgt acttgtctgg aatgatatca tacacatcat
    24481 gtgagtaaac aggtactttt atctcttcct gaccagattt tacacggttc aagaaatcga
    24541 tcagcatctc catatcataa ctttcaggaa atcctttacg gtccatgatt ccacgctctt
    24601 ttaatatacg atttggataa agaaatccgt cagtcgtgat cagctgaacg tttcttcgtt
    24661 tgaaggtacg agacaaaatc gtctgcagca gtctggctgt tgtacttttt ccaacggcta
    24721 cacttccggc gatcccgata ataaaaggcg gtacttctac gtatttatga agaaataaac
    24781 ctttactcaa gaacaaagat tcaaattctt tcatataaag ataaatcaga tgagcaagag
    24841 gtacatagat atctcgaaca tcctgcatgg aaatgcggtc attcaaactt ttgatattat
    24901 ctagttcatc ttgtgtcaaa ggtgcttgcc cattacgata aaattctcgc cattcttctc
    24961 ttgccacacg gtagtagttc attggatcat tcatattagc ctcctgcgtt ctcccatcaa
    25021 tggaactatt ttaacacaaa ataaacgcaa accccaccac ttaagagaga ttgccgatct
    25081 ttttgtataa tctttataat agaaagaaac ggagtcttat tcttgacttg cgcacggata
    25141 cataaaagga tagactgttg tcatgaaaga aatgagaagg atggaagaca tgacaaatca
    25201 ttactattct gaacgcccag atacggcgca cgattttgaa caatgggctt ttgagttaaa
    25261 aggcagaacc tttcaatttg taacagattc tggtgtattt tcacgtgaaa cagtggatta
    25321 tggttcacgt gtcctgattg atgctttcga ttgggaagag ttacctgatg aaggaaagat
    25381 cttagatgtg ggatgcgggt atggaccgat tgggttagct ttggcatttg cgacacagcg
    25441 gtttgttgaa atggtcgaca ttaatgaacg agcggtaagt ctagcgcaag gaaacgcaaa
    25501 gcgtaaccaa atcgaacatg ttgatatcca tcaatcgaat atctatgaag cagttcatga
    25561 agaaacctat gcagctatcg taagcaatcc gccgatccgt gctggaaaaa aagttgtaca
    25621 tgaaattttg acaggcgcat atcctcggtt gaagaagggc ggtacactga caattgtgat
    25681 ccagaagaaa caaggagcgc cgagtgcgca aaagaaaatg gaagaaacat ttggtaatgc
    25741 agaaatcgtc acaaaagata aaggatatta tatcatcaaa agtgtgaaag aataactagc
    25801 tgaaaaatag tggagcgacg aaagagtgga actgggcgtg catgtacggg tacgtatcag
    25861 ttcttttttt gttgagaagt ttttaaaaac aaagaactat tttttcttca aaaagaaata
    25921 gtaggaacag agtcgttgtt ttttacgata aaataaataa gatacagaac ttgtatagaa
    25981 ggctgccaag tcggtagata aaggaggttt ttatcaaaaa acatactttc cttttttctc
    26041 tattcttttc ttgggggttt tggtacactg atagggaatt tagatagaag ggagccaata
    26101 ggatgaagct gaccatcaga gatatcgcag aaatggcagg cgtatcagta acaactgtct
    26161 cacagatttt gaataacaaa ggaagcagat ttagtgataa gacaagaaaa aaagtgttgt
    26221 cgatcgtgaa tgaacatcat tataagcccg attatttcgc ttcaaatgtg atcaatcgac
    26281 attcaaagac catcggaatg atcgtccctg atgtcactga tttcttcttc tccaaactga
    26341 tcgaaggtgt agagagctat ttgaatcctt tgggctatgt gatcatgtta tgcaattctc
    26401 gtcatagcca agaaaatgag atcaagtatt tacaagagct ttcccaccgc tcagtggacg
    26461 gaatcttgct ggccacacca aatattttac ctgagaaata tgcattagac agcggttttt
    26521 atcaaaaaat gccaatcatt ttaatcgatc gcggattgaa tcgtcgggat aacggacggc
    26581 ttatcgtaaa ggaatatgag ggcgcctatc aagctgtttc gctgtttatt gaaaatggcc
    26641 atacgaagat cggcatgctg aaggaaacca ctggatacta tcaattagaa gaacgtttca
    26701 acggatatcg tcatgcgctg aaagatcatg gcctgccttt taatgggaaa tttgttgaac
    26761 atggagagtt gactgtccaa ggtggatata ctgcatcaaa aaaattactc aaacataaag
    26821 atatcacggc tattttttgc gggaatgatg ccatggcgat cggatgctat caggcgatcg
    26881 atgaattggg caaaaaaatc ccagaagata tctcagtcat aggattcgat ggattaaagc
    26941 tgtcagaata catgattccg caattgacga cagtgcagca gccaagtttt gatattggat
    27001 tttatgctgc acgtttcctg atcgatacga tcgaatttcc tcaaagaaaa gttccgaaca
    27061 aagtgttcga aacaaaatta attattcgtg agagcgtaaa agtattgaca aagggctaac
    27121 tcggcggtat attgcttatg taagcattta caaaggctag ttagtgcttt ttttattttt
    27181 agtaaaccag tttactaaag tggtttacct ttcctaaaaa taatgtataa taccgaatga
    27241 agacaaggag gatcaggcaa tgcgaatggt ggatctgatt gaaaaaaaga gggatggaaa
    27301 cgagctgtcg aaagaggaaa tcgagtacat cgtcacgaac tatacgaatg gaaagattcc
    27361 agattatcaa gtcagtgcat tattgatggc aattttttat caagacatga caaatgaaga
    27421 aatcacgaac ttaacattgg cgattgctaa ttctggtgat gtcatcgatt tgagttctct
    27481 tgaaggaata aaggtagata agcattcgac aggtggcgtt ggtgacacga ccacattgat
    27541 tttagccccc ctagtagcta gtgtcggagt gacagtggct aaaatgtctg gacgcggtct
    27601 aggttacaca ggtggtacac tagacaagct agaagcaatt cccggatttc agatcgagct
    27661 ttcggatgaa gaatttgtac gcattgttaa tgaaagtaaa gtagcggtaa ttggtcaatc
    27721 aggaaacttg gcaccggcgg acaaaaaatt gtatgccttg cgtgacgtca cagcgaccgt
    27781 tgattctctt ccgctgattg ccagttcgat tatgagtaaa aaaatcgcgg caggagcaga
    27841 tgcaatcgtt ttggatgtga ccactggaga cggtgctttc atgaagaaca tcgaagatgc
    27901 aagacgtctg gcaaagacca tgacgagcat tgggaaactg gctaaccgag aaacagtggc
    27961 ggttatctca gatatgtcag aacctcttgg cgaagctatc ggaaatagtc tagaagttgt
    28021 cgaggcaatc gaaacattac aaggaaatgg tccagaagat ctagtagaaa tgtgctacgc
    28081 cttaggaagc caaatggttg ttcttgctgg caaagctaag acaaccgatg aagcaagaac
    28141 gttgcttcaa gaagctctgg aatcaggtaa agcgttagca aaattcaaag aaatgatcca
    28201 aaatcaaggg ggagacccag cgatcgtgga acatccagaa cgaattttga ctgcacgcta
    28261 cacaatggag cttcctgcta aacaaagcgg agttgtgtca aaaatcgtgg caaatgaatt
    28321 aggaattgct gcaatgatgc taggtgctgg gcgtaaaacc aaagaagaag atattgatca
    28381 cgctgttgga ttgaaactgc ataaaaaaat tggtgacact gtgacaaaag gtgagtcact
    28441 attgacgatc tatagtaacg acgaagaaat cacttctgtc attgaattat tatataaaaa
    28501 tattgaaatt ggtgaatcgg caatgaagcc aacgttgatt catgacatca tcacagaata
    28561 aaggaaaaaa gcggacttta ccgttttaaa gaaaagggga aaagaaatgg aactaaaccg
    28621 aatgatcgac catactattt tgaaagcaga cgcatctaaa gaagctgtga tgcaaattat
    28681 cgaagaagca aaaaaatacc atttttattc tgtctgcatc aatccagcat gggttgcgct
    28741 agcgaaagaa caattacaag gtgagccagt agccgtatgt acagtaatcg ggtttcctct
    28801 aggagcaaac acacccgaga caaaagcatt tgaaacaaca gatgccatca ataacggtgc
    28861 agatgaagtc gatatggtca tcaatatcgg tgctttgaaa tcaaaggatg atcaacaagt
    28921 ccaaaaagat atcgaagcag ttgttgaagc agcaaaagat cgtgcgcttg tcaaagttat
    28981 tattgaaaca tcgttattag atagagaaga aatcatccga gcttgcgaaa tagcaaaagc
    29041 tgcaggcgct gattttgtca aaacatcaac tggtttctca acaggcggag caaaggtaga
    29101 ggatgtccgt ttgatgcgtg aaactgtagg accagaaatg ggtgtcaaag cttccggcgg
    29161 tatccataac gaagaagaag caatggcaat gatcgaagct ggtgctacaa gaatcggaac
    29221 aagcgcagga gtagcaatcg tatctggttc aactggtgaa ggctattaat tttttcaaag
    29281 gagaagctca tggcagaact caaacaagaa tggatcaaca cagctattga agcgttagat
    29341 aaagcctatg ttccttattc ccattttccg gtaggcgctt gtctcgtaac ggaatcaggc
    29401 aagatttatc aaggcatcaa cattgagaat gcatcatttg gattaacgaa ttgtgccgag
    29461 cgcacagctt ttttcaaagc agtatctgaa ggagaaaaaa catttacgca tctagtcgta
    29521 gcggggcaca cccccgatcc tatctctcct tgtggtgcat gtcgacaagt aatggcagaa
    29581 ttctgttccc ctgatatgcc ggttacgcta gtaggagaca atggtgtgac gaaggcaacg
    29641 accgttcgtg cactgctgcc ttatgcattt actgaaaaag atctataact catgttcatt
    29701 cagtcagctg actgttgaaa aataaaacaa ttattttagg aggctttaca gaaatgaaaa
    29761 aagcaaaatt atttggttta ggtgctgtcg cactagcagc agggttattt ttaggtgcat
    29821 gcggcaacaa cggctctact gattcatccg gtggcaaaag ctcatcagac acaacaactg
    29881 cagcattgat cacagacaca ggtggtgtag acgaccgttc attcaaccag tcagcttggg
    29941 aaggactaga aaaatggggg aaagatcaag gactttcacg aggaaatgat ggcttccaat
    30001 atttccaatc ttcaaatgaa tcagattaca tcccaaatat tgaccaagca ttgaatgctg
    30061 gattcaaaac gatttttggt attggttata aactaaaacc agcgatccaa gaacaagcaa
    30121 caaacaatac aggtacgaac tttgttatta tcgatgatgt catcgatgga ttagataacg
    30181 ttgtatctgc aactttcaaa gacaacgaag cttcttatct tgcaggtgtt gcagcagctt
    30241 atacgactga aacaaacgta gtcggcttta tcggcggtgt aaaaggtgaa gtaatcgatc
    30301 gttttgatgc tggattcaaa gccggtgttg atgcaggtgc gaaagaatta ggaaaagaaa
    30361 tcaaagtatt gaatcaatat gctggtgact tctctgctcc agataaagga cgttcaattg
    30421 ctcaaggtat gtatgcacaa aacgcagata tcatcttcca tgcttcaggc ggtacaggta
    30481 acggtgtatt ccaagaagca aaatcattga acgaatcagg cgacaaaaaa gtttgggtta
    30541 tcggtgttga ccgcgaccaa tcagacgaag gcgaatacac attaaacggc gaaaagaaaa
    30601 acttcacatt gacatctaca ctgaaagcag ttggaacagt agtagaagat ttggcacaaa
    30661 aatcagcgga cggtaaattc cctggcggcg aacacactgt ttatggattg aaagaagacg
    30721 gtgttggtct aacagaagga caactttctg acgaagcgaa aaaagcagtt gatgaagcaa
    30781 aagaaaaaat catttcaggt gacgtaaaag ttccagaaac tcctgaagaa aactaaaacc
    30841 atttctatag ccttgttaga cacatgtttg acaaggctat ggtttgatta gaacaagaat
    30901 tgttcattta tccaagctgc cttgtgacag tttggataag ggaacaactc cctgaagtaa
    30961 tgtttgtttc aaaagaagaa cgctcactaa gcgtgaatct tagtcaatcg ttgccctaga
    31021 tagaaatcgt taggatgtga aaaaaaggtg gttcaagaaa atattgtcat agaaatgcag
    31081 aatattacca aagaatttgg aacattcaag gcaaatgaca acatcaattt acaagtaaag
    31141 gcaggagaaa ttcatgcgtt gcttggagaa aacggtgctg gcaaatctac attgatgaac
    31201 gtgctttccg gattattaga gccgacatca gggaaaattt tgatgcgtgg gaaagaagta
    31261 aagatcacaa gtccgaccaa agccaaccaa ttagggattg ggatggtcca tcagcacttt
    31321 atgcttgttg atgcctttac tgtaacagaa aacatcgtgt tgggaagcga gcctagtcgc
    31381 gcagggatgc ttgaccataa aaaagcacga aaagagatcc aaaaagtttc tgaacagtat
    31441 ggattgtcag tcaacccaga tgcttacgtt cgtgatattt cagttggaat ggaacaacgg
    31501 gtagaaattt taaaaacact ttaccgagga gcagatgtac tgatttttga tgagccgaca
    31561 gctgtattga cccctcaaga aattgatgaa ttaatcgtga tcatgaagga attagtcaaa
    31621 gaaggcaagt cgatcatttt gattacgcat aagttagatg aaatcaaagc agtagctgac
    31681 cgctgtacag ttatccgccg tggaaaagga atcggtacag tcaacgttaa agacgtaacc
    31741 tcacaacaat tagccgatat gatggttgga agaacggttt cattcaaaac gatgaaaaaa
    31801 gaagcgaagc ctcaagaagt cgttttgtct attgaaaatc tagtggtgaa agaaaatcgt
    31861 ggattagaag ccgtgaaaaa cttgaactta gaggttcgtg ctggcgaagt acttggtatc
    31921 gctggaatcg atggaaacgg gcagtcggag ttgatccaag ctttgactgg tttgcgaaag
    31981 gcagaaagcg gacatatcaa gctaaaaggg gaagacatca ctaataaaaa accccgaaaa
    32041 atcactgaac atggtgtagg acacgtgcca gaagaccgtc ataaatacgg gttagtccta
    32101 gatatgacat tatctgaaaa tatcgcacta caaacctatc atcaaaaacc ttacagtaaa
    32161 aacggcatgc tgaattattc agtaatgaat gaacatgcca gagaattgat cgaagaatat
    32221 gatgttcgaa caacgaatga acttgttccc gcaaaagctt tatcaggcgg aaatcagcaa
    32281 aaagcaatta tcgctcgtga agtcgaccga gatcctgatc tgttgattgt tgcaaatcca
    32341 actcgtggac tggatgtagg tgcgatcgaa tttattcata aacgtctgat cgaacaaagg
    32401 gacaaataca aagcagtgtt actgatcagt ttcgaattag aagaaatttt aaatgtttcg
    32461 gatcgtattg ctgttatcca tgaaggagaa attgtcggta tcgttgatcc gaaagaaaca
    32521 tctgaaaatg aattaggtct attgatggcg ggctatacct tagaagaagc acgaaaagag
    32581 ttagaaaagg gggctggcgc acatgaatga tcgcagcgaa aaattccgta atttattagt
    32641 gcctgtactt tcagttgttt taggattgat cttaggcgcg atattgatgg ctgcttttgg
    32701 ttttcagccc atccaaggtt attcttctat gctagatgca gcccttggtt cgcaaagagg
    32761 gataggtgaa acgttaaggc aagcaacgcc gttgattttt acagcattag gtttttctgt
    32821 agctaactca gctgggtttt tcaatattgg gctttctgga caagcacttt gcgggtggat
    32881 cgtcagtgtc tgggtggcac ttgcgtttcc agatctgcca aaagttgttt tgcttccgtt
    32941 atgcgtgatc ttaggtgcct tagctggtgc gatggcagcg gctgttccag gtttacttcg
    33001 agcatttttt ggaacaagtg aagtgatcgt aacaatcatg atgaactata tcttattata
    33061 cgtaagcaca tttgctttac atgaagtgat gcctgaaagc tatcgctcaa gtttggactc
    33121 ttccaatatg atcagtcaaa atgcctcact gcggttacca tggttaacac aagcttttgg
    33181 cggctctcgc gtaaactcag gattattttt agcaatcatc gcgcttattt tcgtttggct
    33241 gctgatgaag aaaacgactt taggatttga aatccgttct gttggattga atccttttgc
    33301 ttctgagtat gcaggaatga gtagtaaacg tacgatcgtt ttgtcgatgg tcatttctgg
    33361 cgctttggca gggcttggcg gtgtggttga aggattagga acgtatcaaa acttctttgt
    33421 tcaaacgact tcgctatcta ttggatttga cggaatggct gtttccttgc ttggttctgg
    33481 atcagcaatc ggtattcttt tatcagcgat cttgttcagt attttgaaga tcggtggact
    33541 tggcatgcag acaggagcag gtgttccgtt tgagatcgtc aatgtatcga ttgctttgat
    33601 tatcttcttt gtcgggatca acttcctgat tcgtttcgtc atggctaaag tcttccaagg
    33661 gaaaaaacaa gaagagatcg ttgcgacaat agaatcgaaa ccaacgaatc aaaaccatga
    33721 agggggcgaa ctataatgtc gactatcgaa ttaattgcct tgatccttac ttctacactg
    33781 gtttattcaa caccgctaat tctaacgtca ctaggcggaa ctttttctga acgaagcggg
    33841 atcgtaaacg tagggctaga gggaatcatg gtcatgggtg cttttagttc catcgttttc
    33901 aacttgacga tgagtccatc acttggttct atgacaccat ggatcgctat tttagtgggt
    33961 ggcgtcgtag gtatcctatt ttctctgatt cacgcagtag caacagtcag tttgcgtgct
    34021 gaccatatta tcagcgggac agtaattaat ctgatggcac ctgctttagg tgttttcttg
    34081 atcaaagcat ggtatggtaa aggtcaaact gataacatca cgcagaatct ggggtatttt
    34141 tctttcccag gattagcaga tattccggtc attggaccaa ttttctttaa aaatacattc
    34201 ttacctgctt attgtgctat tttgattgct gtcctagctt gggtgatcat cttcaagacg
    34261 aaattcggct tgcgcctccg ttcagttggt gaaaatcctc aagctgccga cacattaggt
    34321 atcaatgtat atgctatgcg ttattcaggc gtactgattt ctggcttgtt aggcggtatg
    34381 ggcggtgctg tttttgctca gtcgatttcc ggtaactttt cagctggtac gatcgttgga
    34441 caagggttca tctccatggc tgctatgatc tttggtaagt ggaatccttt aggggcaatg
    34501 ggtgcctcat tgttctttgg ttttgctcaa agcttgagta tcattggcgc acaattacca
    34561 gttatttctt cgattccgcc agtattgctg caaattgctc catatttgct gacaatcatt
    34621 gtgctggttg ttttcttagg gaaagcttct ggtccaaaag caaacgggaa gaactatatc
    34681 aaatcaaaat aatcttttag cgaataaaaa gggtgggtta cgggaagtgt ttttcgtctc
    34741 gccctttttg ttttaaaggc aataaaaata aaaagaaaga aggaataatc atgtttaaac
    34801 gcgtacatct gatcgtcatg gattcagtag gaattggaga agcaccagat gccgataagt
    34861 tcggtgatgt tggttctgat acattaggac acattgcgaa agaagcagga ttgaacgttc
    34921 cacatttaga gcagcttggt ttaggaacga tccgtcctct tgaaggtgtg gaatcagtga
    34981 aagatcatca aggctatgcg acgaaattag aagaagtttc tgtagggaaa gatacgatga
    35041 caggccactg ggaaatcatg ggattgaata tcaaaactcc atttcgtgtg tttcctaatg
    35101 gattccctga tgatctgatc aaacaaatcg aagaattttc tggccgtaaa gtcgtatgta
    35161 acaaacctta tagcggaaca gctgttattg acgattacgg tgaacaccaa atgaaaacag
    35221 gcgatttaat tgtatatact tctgccgatc ctgtccttca gatcgcggca catgaagaaa
    35281 tcattccttt agaagaatta tatcggattt gccaattcgt tcgtgatata acaaaagatg
    35341 atccgtatat gattggccga atcattgcac gtccgtacgt gggtgaacca ggagacttca
    35401 cacgaacaag caatcgccat gattatgcgt tgaatccatt cggacacaca gtccttgatt
    35461 ctttgaaaga agcaggaaag gacgtgattg ctgttggaaa gatcaacgat attttcaacg
    35521 gacaaggaat cactgaatct gtccgcacga aaagcaatat ggatggcgtt gaccagctgc
    35581 tgaaagttat gaaaaacgat ttttcaggaa tcagctttac caatttagta gattttgatg
    35641 cattatacgg tcatcgtcgg gatgtagtcg gctatgcgca tgcgattgaa gaatttgact
    35701 tacgaattcc agaattgctt gaagcgatgg aagaagacga cttgctaatg attacagccg
    35761 atcacggaaa cgatccgacg tttactggta ccgatcatac gcgagaatat gtaccgttat
    35821 tggtttacag taaaaaaatg aaagaacaag gggcattgcc tcaaggatat tattcagata
    35881 tttcagcaac gattgctgag aactttagtg tttcttcgac tgaaaatggc gaaagcttct
    35941 tgaaattatt aaaataatct ttttaaatag gagaaagcaa atgaccaagt taagcgagat
    36001 gttacaagaa acgacccagt ttatccttga taaaggagta aaagaaattg attttggttt
    36061 gattctaggt tcaggattag gtgaattagc agatgaaatc gaagatgctg taatgatttc
    36121 ttatgatgag atcccgttct ttcctgtttc gacagtagtt gggcatgcag gaaagctagt
    36181 ttatgggtct ctggcaggca agaaagtgtt agccatgcaa gggagattcc actactatga
    36241 aggtcattcc atgcaaacag tgacctatcc agtacgtgtg atggccggac taggggccca
    36301 ttcagtcatt gtgactaatg catgtggtgg tgtcaacgaa tcatttgtac caggagattt
    36361 gatgctgatt accgatcata tcaattttac tggtgaaaat cctttgatcg gtccgaatga
    36421 agatgcaatg ggccctagat ttccagatat gagtcaagct tatacgcctg actatcaagc
    36481 aacagcaaaa gcagcagcag aaaaactagg tttggatctt aaagaaggcg tatatatggg
    36541 ctattctgga ccaacttatg aaacgcctgc ggagatccga atgtctcgga caataggagc
    36601 cgatgcagta gggatgtcga ctgttccaga agtcatcgta gcagctcaca gcggattaaa
    36661 agtgttagga atctcttgta ttaccaactt agcagcaggt atgcaagcga atctgagcca
    36721 tgaagaagtt gtagaaacaa ctcagagagt caaacaatct tttaaagcat tggttaaaga
    36781 aacattgaca ttattgtaat aggaggaaca ataaaatgag tgtgcatatt gaagcgaaag
    36841 aaggcgagat cgccgataag attttacttc cgggagatcc tttgcgagct aaatatattg
    36901 cggagacttt tttggaagat ccagtttgtt acaatcaggt acgcggtatg ttaggcttta
    36961 ccggcaaata taaaggagaa cgtatttctg ttcaaggaac aggaatggga atgccatcag
    37021 ccaccatcta tgcccatgaa ttgatccagt cgtatggtgt aaagaaattg attcgagtag
    37081 gtacatgcgg tgcattgtca aaagacgtcc atgttcgtga tttagtacta gcacaaggtg
    37141 cagcgaccag ttcttcgatg atcgaaaaaa atttccaagc ctttcatttt cctccaatca
    37201 gtgatttcaa tctattactg aaagcatatg agatagctaa agaaaagggc tatacggttc
    37261 atgtaggaaa tgttctttcg gaagattctt tctacaaaga tgatttgaca gagacgttcc
    37321 aattagctga acttggcgtt ttaggagtgg aaatggaagc agcggctctt tattacttag
    37381 gcgcgaaata ccatgtgcag acattaagtt tgatgaccgt tagcgaccat ttgatcaccg
    37441 gtgaagaaac aacagctgca gaacgtcaat ctacgttcaa tgaaatgatc gaagtaggat
    37501 tggaaacagc gatagcagaa taagaaggaa atgaaaaagc tgccctgcat taagtaataa
    37561 gaacatccaa gcaaaaatag ctcctcatgt ttttgacttg gctggactta ttattgcaaa
    37621 tgtaagcttt tgaacaccgt ttaccaagag gttgtgaatc tgccgtttaa ctctgagtca
    37681 caaggaacaa tttcaaaaaa cagcttttca tgtttgatga atattgtgac ttgtgaccga
    37741 gaagttggca ggagaaaacc gtttatacga aaaaagggac tgtgacaaat cgtgtcacag
    37801 tccctttttt atagtgtttt ttataggtag tattttttag caactgttaa gtcatcattt
    37861 aattcataaa caagtggttg acctgttgga atttcaagat ccatgatgtc ttcgtcagag
    37921 ataccttcga tatgtttagc tagggcgcgt aatgagttac catgtgcagc tactaaaaca
    37981 gttttgttgt ctagtaatgc tggagcaatt tcatcttgcc agaatggcaa agcgcgttct
    38041 aatgtcactt tcaagttttc tccaccagga atatcgcgtt tgtctaacat tgcgtaacga
    38101 cggtcatttg ctgcagaacc ttcgtcagtt tcttccatta atggaggaag agtatcgtaa
    38161 gaacgacgcc agatgtgcac ttgttcgtcg ccgtattttt cagctgtttc ttttttgttc
    38221 aaaccttgta atttaccata gtgacgttcg tttaagcgcc atgatttgat ttgtggtacc
    38281 caaagttgat ctgaatattc taagatcaag ttacatgttt tgattgcacg agttaaaaca
    38341 gaagtgtaag caacatcaaa ttcgattcca gcttctttga ttttgcgtcc gccttctttt
    38401 gcttcttcga ttccttctgg tgctagattt acatcagccc aaccagtaaa ttggttcaat
    38461 gcattccatt cactaagacc gtgacgagaa aaaactaatt ttggcatgtt tagctctcct
    38521 tttaagatag gtttatattt ctgaaatttt cagttctgtt ttattgtaca attttttgga
    38581 agagatttcc atcgaaaaat tagataagat tctatgagaa aacaagtaga gaatataaaa
    38641 aaacgaacag tgcccacgca actgttcgtt tttaggtatt agatgcataa ttgctcgatt
    38701 tctttttcag gtaaattgat atatttataa catgttccaa ctgaaacacc gcattttgaa
    38761 gaaataaatt ggatcgtttc ttttttttca taatagagac gtcggatttt tttgatcgtt
    38821 tttgcatcga tttttggtct tccgccgatt tttccttttt tacgagcttc atctaatcca
    38881 actaatgtac gttctttgat caatgcgcac tccatgtttg ctaggttttc cattaattgg
    38941 aaatagattt ttcccatttc gcgcgtatcg attccttcat caagactaat caagtgcagc
    39001 ccgcgatctt tgaataattg cgtgagttct gttaattgcc gcgtagattt gcctaatcga
    39061 tgaagttggc aaatgactag tgcatcgcct tgttgcatgt cagcgatcac tgactccaaa
    39121 tgaacagcag tagaatcatt ttgttccact tcatatgtct catggaaaat ttcttgacaa
    39181 ccaaagtcag ataattgatc aacctgagtt gttaaatcaa cgtcatttat ggttgtgcgt
    39241 gcataaccaa taattttcat ggtgattcct ttctagcagt gattttctaa ctggatataa
    39301 cagtaaaaat gtagcataaa gtacgtcaaa aagatagttt tttcgcaaat tttccgctgt
    39361 ctttttttat tggatgatca agcagttttt ctttatttac gacggattgc gtcaagaatc
    39421 tcaacgcctt tttctgagcc gacaactact ttagaaacca tgtttaggaa aagcccatgt
    39481 tctactaccc caacaagttc atctaaatag gtagctaagc tttctggctg ttcgatgact
    39541 tctagatgca gatcaatgat gtaatgacca ccatctgtgg ttaagacttc tccgttttcg
    39601 tctgtacgca agacgggttg gaatcttttt tcttcaaaaa tatgaagtag ctgttgggag
    39661 ccataaggga tcacttcaac tgggagtggg aacttcccaa gtttatcgac taatttagaa
    39721 gaatcaacga tccaaatcgt ttcttttgag tacgtagcga ctattttctc gaaaagcaaa
    39781 gcagcgccgc cgcctttgat cccttgaaaa tcagcactga tttcatcagc accgtctatt
    39841 gtcaaatcga ctacggggac ttcatcaata ctctttaacg gtattcctaa tttttccgct
    39901 tgttcttttg tcgcatttga agtagtaact cctgtgatac gtaaaccttc ttcacgcata
    39961 cggcgaccaa tctcttctac catataatag gcggttgaac cggtcccgag acctacgatc
    40021 atcccgtcct ttacgaattt cgcggcttca atgccagcta attgttttaa attcatcttt
    40081 gcgtccacct ttttttagtt tgagcatcgt gctttcctca ctattttagc gtgatttacg
    40141 gtaaaaagat accattcttt tttattccaa taaagctttt tcaaaaaaaa ttgcatctct
    40201 tgttgacatg ttttttcatt cgtgatattc taacaaaggt gctttatcta caggtttctg
    40261 tgaagaaacg tgctgactgt ttatcaaagc atatttgata aggatgcaga cattgcattt
    40321 attttttatc acaaaaaaag aaccacctgg atgtgtggaa ctagacgcaa atcgtgggaa
    40381 ggaggaaaca aggaatgcct acaattaacc aattagtacg taaacctcgt aaatcaaagg
    40441 tggaaaaatc taattcacct gctttgaaca aaggatataa cagctttaag aaaactcaaa
    40501 ctaacgtaaa ctcacctcaa aaacgtgggg tagcaactcg tgtgggtaca atgacaccta
    40561 aaaaaccgaa ctcagcttta cgtaaatatg cacgtgttcg tttgtcaaac ttaattgaag
    40621 ttactgctta tatcccaggg attggtcata acttacaaga acacagtgtt gtattattac
    40681 gcggtggtcg tgtaaaagac ttaccagggg tacgttacca tatcgttcgc ggtgctttag
    40741 atactgctgg tgttaacgac cgtaaacaaa gccgttctaa atacggaact aaaaaaccta
    40801 aagcttaatt ttaattatta aacaaatgaa acttgcttct actatataga agtaattact
    40861 atgaacaata tttcggaagg aggagttacg gatgccacgt aaaggtcctg ttgcaaaacg
    40921 tgatgtttta ccagatccta tttataactc aaaattagta actcgcttga tcaaccgtgt
    40981 aatggttgac ggaaaacgcg ggattgcagc taacattatc tataatgcct ttgacttgat
    41041 caaagaatct acaggtaacg atccattaga agtgtttgaa caagcaatga aaaacgttca
    41101 acctgttctt gaagttaaag cacgtcgtgt tgggggttca aactaccaag taccagttga
    41161 agttcgccct gaacgccgcg ttactctagg tcttcgttgg gtagtaaact acgctcgctt
    41221 gcgcggtgaa cacacaatgg aagaacgctt agcaaaagaa atcatggatg ctgcaaacaa
    41281 cacaggtgct tctgttaaaa aacgtgaaga cactcataaa atggctgatg ccaaccgtgc
    41341 gtttgctcac tatcgctggt aagattctct ctgtttggtc ggcttttata agtcgacctc
    41401 agatagtgtc cttattttta gcaactagat agtaaagaga ggagaaacaa ggagaatgac
    41461 cagagaattt tctttacaaa acactcgtaa tatcggtatc atggcccaca tcgatgctgg
    41521 taaaacgact gctactgaac gtatcttgta ctatactggt aaaatccata aaattggtga
    41581 aactcatgaa ggagcttcac aaatggactg gatggaacaa gaacaagaac gtggtattac
    41641 gatcacttct gctgctacta cagcacaatg gaagggtcac cgaatcaaca tcatcgacac
    41701 accaggacac gtagacttca cagttgaagt tgaacgttca ttgcgtgtac ttgatggtgc
    41761 tgtaactgtt cttgatgcac aatcaggtgt tgaacctcaa actgaaacag tttggcgtca
    41821 agcaacaact tatggtgtac ctcgtatcgt atttgcaaac aaaatggaca agatcggtgc
    41881 tgacttctta tattcagtat caactttaca tgaccgtttg caagcaaacg ctcacccaat
    41941 ccaattacca attggtgcgg aagacgactt cacaggaatt atcgaccttg taacaatgaa
    42001 ggctgaaatg tatacaaacg atcttggaac agagatcgaa gaaacagaaa ttcctgaaga
    42061 ataccgcgaa ttagctgaag aatggcgcga aaaattggtt gaagctgtag ctgaaactga
    42121 tgaagaactt actttgaaat acttagaagg tgaagaaatc actgaagctg agttaaaaga
    42181 aggtatccgc cgcgcaacag ttaacgtaga attctaccca gtactatgtg gttcagcatt
    42241 taagaacaaa ggggttcaat tactattgga cgctgttctt gattacttgc catcaccgtt
    42301 agatatccca gctatcaaag ggattgaccc taaaactgat gaagaagtag aacgtcctgc
    42361 agacgacagc gctcctttct cagctttagc atttaaagtt atgactgacc cattcgtagg
    42421 tcgtttaact ttcttccgtg tgtactctgg tgttttacaa tcaggttcat acgtacaaaa
    42481 cgcaacaaaa ggcaaacgtg aacgtgttgg tcgtatccta cagatgcatg ccaactctcg
    42541 ttcagaaatc tctgaagttt acgctggaga tatcgctgct gctgttggtt tgaaagacac
    42601 aactactggg gacacactat gtgatgaaaa gagtctagtt attcttgaat ccatggaatt
    42661 cccagaacca gttatccaag tcgctattga acctaaatca aaagctgacc aagataaaat
    42721 gggtgtggct ttacaaaaac tatctgaaga agatcctact ttccgtgctg aaacaaacgt
    42781 ggaaacaggc gaaacaatca tcgctggtat gggtgaactt cacttagata tcatcgttga
    42841 ccgtatgcgt cgtgaattca aagttgaagc taacgtaggt gcgcctcaag tatcttaccg
    42901 tgaaactttc cgtgctggca ctcaagccga aggtaagttt gtacgtcagt ctggtggtaa
    42961 aggtcaatac ggtcacgtat ggattgaatt tacaccaaat gaagaaggtg ctggtttcga
    43021 atttgaaaat gcgatcgtcg gtggggttgt tcctcgtgaa tacattccag ctgttgaaac
    43081 tggtttgaaa gatgctatgg aaaatggtgt cttagctggc tatccattag tagatatcaa
    43141 agctaaactt tacgatggtt cataccatga tgtcgattca aatgaaactg ccttccgtgt
    43201 tgctgcttct atggcattac gtgcagcagc taagaaagct aatccagtaa tccttgaacc
    43261 aatcatggct gtagaagttg ttatccctga agattattta ggtgatgtta tgggtcacgt
    43321 aactgctcgt cgtggacgtg ttgaaggtat ggaagctcgc gctggcggcc aacaagttgt
    43381 tcgtgcgatg gttccacttg ctgaaatgtt tggttatgct acaactcttc gttctgctac
    43441 tcaaggtcgt ggtacattta caatgacatt tgaccactac gaagatgtac caaaatcagt
    43501 acaagaagaa atcattaaga aaaacggcgg aaaagctgaa taatttcttg aaaccggata
    43561 gtcctgcaaa gagattatcc aaagagacta tatttttaat atagtttcgt gtaaaataat
    43621 tatcgatgat atgggcatgt ggtgtacctt gtgtacatca tatacctatc agacaaataa
    43681 aaaatatatt gtatttttag gaggacattt taaaatggca aaagaaaaat ttgaccgttc
    43741 taaaccacac gttaacattg gtactatcgg acacgttgac catggtaaaa ctacattaac
    43801 agctgcaatc acaactgtac tatctaagaa aaacggcggc caagctatgg cttacgatca
    43861 aatcgatggt gctccagaag aacgcgaacg cggaatcaca atctcaacag ctcacgttga
    43921 gtatgaaaca gatacacgcc actacgctca cgttgactgc ccaggacacg cggattacgt
    43981 taaaaacatg atcactggtg ctgctcaaat ggacggagct atcttggtag tttctgctgc
    44041 tgacggccca atgcctcaaa ctcgtgaaca catcctattg tctcgtcaag ttggtgttcc
    44101 ttacatcgtt gtattcttga acaaagtaga catggttgat gacgaagaat tactagaatt
    44161 agttgaaatg gaagttcgtg acctattaac agaatacgaa ttccctggtg acgatgttcc
    44221 tgtagttgct ggatcagctt tgaaagctct agaaggcgac gcttcatacg aagaaaaaat
    44281 tcttgaatta atggctgcag ttgacgaata catcccaact ccagaacgtg acaacgacaa
    44341 accattcatg atgccagttg aagacgtgtt ctcaattact ggacgtggta ctgttgctac
    44401 aggtcgtgtt gaacgtggac aagttcgcgt tggtgacgaa gttgaagttg ttggtattgc
    44461 tgaagaaact tcaaaaacaa cagttactgg tgttgaaatg ttccgtaaat tgttagacta
    44521 cgctgaagct ggagacaaca ttggtgcttt actacgtggt gttgcacgtg aagacatcca
    44581 acgtggacaa gttttagcta aaccaggtac aatcacacct catacaaaat tctctgcaga
    44641 agtatacgtg ttgacaaaag aagaaggtgg acgtcatact ccattcttca ctaactaccg
    44701 tccacaattc tacttccgta caactgacgt aacaggtgtt gttgaattac cagaaggaac
    44761 tgaaatggtt atgcctggcg acaacgtaac aatggaagtt gaattaatcc acccaatcgc
    44821 tatcgaaaac ggtactaaat tctcaatccg tgaaggcgga cgtacagttg gtgccggtgt
    44881 cgttacagaa atcaaagctt aattcactta agttttaaag taaagcaaca tttattcgga
    44941 cgtaatcgct cggaacgaag gtcttaggat ctttgttccg agctttttta tttattgcag
    45001 gatcaggaaa aaagacagga gtattataaa tagaagaaac acgaaataaa agagtcagta
    45061 ttttctaaat gaaactttat gaaaacaaaa tgttttttac aagaaaaagc cttgctttgt
    45121 gagtttatat atattataat gacaaaggtg cgtttacttt agtgaattgc acagacgtaa
    45181 tcagaatgat ttcgtcggct aagaaacgag aggttgcgac acgcccggac gctttgccac
    45241 gaacgagcgt gacggaaatt ttcgtggagc tatgtctact tttcaaaata ggcgaaggag
    45301 ggaacaagat ggcaaaacaa aaaattcgta tccgtttaaa agcgtatgaa caccgtattt
    45361 tagatcaatc agcggataaa atcgtggaaa ctgcaaaaag aactggagct agcgtgtctg
    45421 gtccaattcc attaccaact gaacgcagtc tttacacaat catccgtgcg actcataaat
    45481 acaaagattc acgcgaacaa ttcgaaatgc gtacacacaa acgtcttatc gacattgtga
    45541 acccaacacc aaaaactgtt gatgctttga tgaagctaga cttaccatct ggtgtaaaca
    45601 tcgaaatcaa attataaaac taaaacaaaa ataatggagg tgtactcatg accaaaggaa
    45661 tcttagggaa aaaagtggga atgacacaaa tcttcactga atctggtgaa ttaattccag
    45721 taactgttgt agaagctact ccaaacgtag tactacaagt gaaaacaatg gaaacggatg
    45781 gctacgaagc tattcaagtt ggttaccaag atatgcgtga agttttatca aacaaacctg
    45841 cgaaaggtca tgttgcaaaa gcaaacacgg ctcctaagcg cttcattcgt gaattcaaaa
    45901 atgttgagct aggagaatat gaagtaggaa aagaaattac agtagatgtt ttccaagcag
    45961 gagacattat tgatgtaaca ggtactacga aaggtaaagg attccaaggg gttatcaaac
    46021 gccacggaca aagccgcgga ccaatggctc acggttctcg ttaccatcgt cgtcctgggt
    46081 caatgggtcc agttgcacct aaccgtgtat ttaaaaacaa acgtcttgct ggccgtatgg
    46141 gtggcaaccg cgtaactatc caaaacttgg aagttgtacg tgtcgatgca gaaagaaatg
    46201 ttatcttgat caaaggtaat gttcctggag ctaaaaaatc tttaattact atcaaatcag
    46261 ctgtgaaagc taaataattg taggaagaaa ggaggaacta aggaatgccg aatgtagcat
    46321 tattcaaaca agatggaagc caaaacggtg aaatcacact taacgaagaa atcttcggaa
    46381 ttgaacctaa tgaaagtgtt gtttacgatg caatcgtaat gcaacgtgcg tcattaagac
    46441 aaggaacaca cgcggttaaa aaccgtagcg ctgttcgcgg cggcggacgt aaaccatggc
    46501 gtcaaaaagg aacaggtcgt gcacgtcaag gatctatccg ctcaccacaa tggcgtggag
    46561 gtggcgtagt ctttggacca acaccacgtt cttacagcta caaacttcct aaaaaagttc
    46621 gtcgtttagc aatgaaatct gtattgtcag aaaaagttgc tgaaaacaac ttggtagcaa
    46681 tcgaaggctt gaacttcgac gcaccaaaaa caaaagaatt caaacaagtt cttgctaact
    46741 tatctatcga ctctaaagta ttggttgttc tagaaaaagg taacgacttt gctgctttat
    46801 cagcacgtaa cttaccaaac gtttctgtag taacttctga taatgtgagt gtgctagacg
    46861 ttgtatcaaa tacaaaagtg ttagcaacac aaactgctct tactcaaatt gaggaggtgc
    46921 ttgcataatg aacttactag acgtaatcaa acgcccagtg atcactgaaa aatcaatgct
    46981 tgccatggat gacaagaaat acacttttga agtggacact cgcgcaaaca aaactttagt
    47041 aaaacaagcg gtagaagctg cttttgacgt taaagtcaaa aacgtgaaca tcgtgaacgt
    47101 acgtgcgaaa ttcaaacgta tgggcaaata tgcaggatat acaaaaaaac gtcgcaaagc
    47161 gatcgttaca ttgactgaag attcaaaaga aatccaatta ttcgaagctg ctgaataagc
    47221 actaatagat aactcattaa attaggaggg aaaaacgtgg cgattaaaaa gtacaaacct
    47281 acctcaaatg gtcgtcgtaa catgacaggt tctgattttg ctgaaatcac aacatcaaca
    47341 cctgaaaaat cattgttaca accattgaaa aaccatgctg gacgtaacaa caacggccgc
    47401 atcactgtac gtcatcaagg tggcggacac aaacgccaat atcgcgtgat cgacttcaaa
    47461 cgtaacaaag ataacgttgt tgcaactgtt caaacgatcg aatatgatcc aaaccgttca
    47521 gctaacatcg cgttagtaca ctatgaagat ggagtgaaag cttacatctt agcacctaaa
    47581 ggactagaag tgggtatgaa acttgtttca ggtccagaag cagatatcaa agtcggaaat
    47641 gctcttccat tagaaaacat tccagttggt actgttatcc acaacattga aatgaaacct
    47701 ggtaaaggtg gacaattgat ccgttctgct ggtacaagtg ctcaagtact tggtaaagaa
    47761 ggcaaatacg tattgatccg cttgaactct ggtgaagttc gtatgatctt agctgcatgc
    47821 cgtgctacaa tcggttctgt aggtaacgaa caacacgaat taatcaacat cggtaaagct
    47881 ggacgctctc gttggatgcg taaacgccca actgtacgtg gtagcgtaat gaaccctaat
    47941 gatcacccac acggtggtgg tgaaggtaaa gctcctatcg gacgtaaagc accagtttct
    48001 ccttggggtc aaccagctct tggttacaaa actcgtaaca aaaaagctaa atcagacaaa
    48061 cttattgttc gtcgtcgcaa aaataaataa ttactttgac catgtgtcgc acattttgag
    48121 aggaggttca ccatgggtcg tagtttaaag aaaggacctt ttgtcgatga gcatttaatg
    48181 aaaaaagttg aagctcaagc aggggttgaa aagaaaaaag ttatcaaaac ttggtcacgc
    48241 cgttcaacag ttttcccaag ttttgttgga tataccatcg cagtttatga tggacgtaaa
    48301 catgtaccag tttacatcca agaagacatg gtaggacaca aattaggtga atttgcacct
    48361 acaagaactt atcgtggaca tgctgctgac gataagaaaa cgaaacgcta atttgagggg
    48421 aggataaatc caaatggcag aacaaatcac atcagctaaa gcaactgcaa aaacagttcg
    48481 cacttcacct cgaaaagccc gtttagtaat tgaccttatc agaggtaaaa gtgtcgcaga
    48541 tgcaatttca atcttgaaat tcacaccaaa caaatctgct ggaatcattg aaaaagtatt
    48601 gatgtcagca gttgcgaatg cagaaaataa ctttgactta gatgttgaaa acttggtagt
    48661 aagcgaagct tttgttaacg aaggacctac aatgaaacgt ttccgtcctc gtgcaaaagg
    48721 atcagcttct ccaatcaaca aacgtacaag tcatatcacg gtagtcgtat cagaaaaata
    48781 aggagggata atcagtgggt caaaaagtac atccaattgg aatgcgtgta ggcatcatcc
    48841 gcgactggga tgctaaatgg tatgctgaaa aagagtatgc tgagttctta cacgaagatt
    48901 taagaatccg taaatttatt tcaactaaac ttgctgatgc tgctgtatca acagttgaaa
    48961 tcgaacgcgc aacaggtcgc gtaaacattt caattcacac agctaaacca ggtatggtta
    49021 ttggtaaagg cggttctgaa gtcgaaagcc taagaaaaga attaaacaaa ttaactggta
    49081 aaagaatcca tatcaatatc gtggaaatca aaaaaccaga tctagatgca aaattagtag
    49141 gtgaaggaat cgcacgtcaa ttagaaaacc gtgttgcttt ccgtcgcgct caaaaacaag
    49201 ccatccaacg ctcaatgcgt gctggcgctc aagggatcaa aactcaagta tctggtcgtc
    49261 taaacggagc agatatcgct cgttcagaag gatactcaga aggaactgtt cctcttcata
    49321 ctttacgtgc ggacattgat tacgcatggg aagaagcaga tacaacatac ggaaaactag
    49381 gagttaaagt gtggatctat cgtggagaaa ttcttccaac taaaaaaaac actgagaaag
    49441 gagggaaata atcatgttag tacctaaacg tgtaaaacac cgtcgtgaat tccgcggaaa
    49501 aatgcgcggg gaagctaaag gcggaaaaga agtagcattc ggtgaatacg gtttgcaagc
    49561 tgttgattca cattggatca caaaccgcca aatcgaagct gctcgtatcg caatgactcg
    49621 ttacatgaaa cgtggtggga aagtatggat taaaattttc cctcacaaat cttatactgc
    49681 caaagcaatt ggggtacgta tgggttctgg taaaggggca cctgaaggat gggttgcacc
    49741 agtaaaacgt ggtaaaatca tgtttgaaat cgcaggcgtt cctgaagaag tagctcgtga
    49801 agcgttacgt ctagcttctc acaaattacc aatgaaaact aagatcgtaa aacgtgagga
    49861 aatgggtggt gaatcgaatg aaggttaaag aaatcagaga attaaccact gccgaaatgc
    49921 ttgatcaaga aaaacaatta aaagaagaat tgtttaatct tagattccaa ttagcaacag
    49981 gtcaattaga aaacactgca cgtattaaag aagtacgtaa atcgattgca cgcatcaaaa
    50041 ctgtgttgcg tgaacaagct aagtaattat ggaaggaggc cattaagcgt atgactgaag
    50101 gaagaaatca acgcaaagtt tatcaaggtc gcgtggtttc agacaaaatg gataaaacaa
    50161 ttaccgttgc tgtcgaaaca aagaaaaacc accctatcta tggcaaacgc atgaactatt
    50221 ctaagaaata caaagtgcat gatgaaaaca acacagcaaa agttggcgac atcgtgagaa
    50281 ttatggaaac tcgtccatta tcagctacaa aacgtttccg tttgttggaa gtagtcgaag
    50341 aagcagttat tatctaataa cacgagattt tcctatatag attgaatgca aaatctgaaa
    50401 ggaggataca caacgtgatc caagcagaaa gtcgtttaaa agtcgctgat aactcaggcg
    50461 cacgtgaaat tttgacgatc aaagtccttg gtggatctgg tcgtaaaact gctaacatcg
    50521 gtgatgtgat cgttgctacg gttaaacaag caacgccagg tggggttgtc aaaaaaggtg
    50581 acgtcgttaa agccgttatc gttcgaacaa aatcaggagc tcgtcgtaca gacggttcat
    50641 atattaaatt tgatgaaaat gctgcagtga ttattcgtga tgataaaagc cctcgtggaa
    50701 cacgtatctt tgggccagtt gcacgtgaac tacgtgaaaa caacttcatg aagatcgttt
    50761 cactagcacc agaagtttta taatcttgag acacttatca aaggaggtgc gaaacagtta
    50821 tgtttgttaa aaaaggcgat aaagttaaag ttatcactgg aaaagacaaa aacaaagaag
    50881 gcgttgtact agcagcgttc ccgaaaaaag ataaagttat tgtcgaaggt gtaaatgtcg
    50941 ttaaaaaaca ccaaaaacct tctcaagctg ctcctcaagg tggaattgtt gagatggaag
    51001 cgccaattca tgtttctaac gtgatggtgg ttgactctac tggtgtagct ggccgagttg
    51061 gctacaaaga agtagacggc aaaaaagtcc gtgtttctaa aaaaaccggt gaagttttag
    51121 ataaataaaa gtttaaggaa ggagggtctt actgaatgaa ccgcctaaaa gaaaaatatc
    51181 ttaaagaagt aactccatca ttgatggaaa aatttaacta tagctctgtt atgcaaacac
    51241 ctaaagttga aaaaatcgtc atcaacatgg gtgttggtga tgcggtatca aacgctaaaa
    51301 acttagataa agcagttgaa gaattaactt tgatcactgg tcaaaaacca atgattacaa
    51361 aagctaaaaa atcaatcgct ggattccgtt tacgtgaagg aatgccaatt ggtgcaaaag
    51421 ttacattgcg cggagaaaga atgtacgaat tcttagacaa attggtttct gtttcactac
    51481 cacgtgtacg tgacttccat ggtgtaagta aaaaagcctt tgacggtcgt ggaaactata
    51541 ctttaggtat taaagaacaa ttgatcttcc cagaagtaga ttatgattta gtagataaag
    51601 tacgtggtat ggacatcgtt attgtaacaa cagcaaactc tgatgaagaa tctcgtgagt
    51661 tgttgacaca attaggcatg ccattccaaa aataattaaa ggaggcgaac tacgtggcta
    51721 aaaaatcaat gattgctaaa aacaaacgtc ctgcaaaatt ttcaacacaa gcttatactc
    51781 gttgcgaacg ttgcggacgt ccacattcag tttatcgtaa gttccatctt tgccgtattt
    51841 gcttccgcga acttgcctat aaaggtcaaa ttcccggcgt gaagaaagct agctggtaaa
    51901 aagtaaattc gcataagaag gaggtaaaag atcaatggtc atgacagatc caattgcaga
    51961 ttttctaact cgtatccgta acgcaaacat ggttaaacat gagtctttag aagtacctgc
    52021 atcaaaaatt aaacgtgaca tcgctgaaat cttgaaacgt gaaggtttcg ttcgtgatgt
    52081 tgaatacatc gaagatgaca aacaaggcgt gatccgtgtt ttcctaaaat ttggtaaaaa
    52141 cggcgaacgt gttatcacaa acttaaaacg tatctctaaa ccaggtttgc gtgcttatgt
    52201 caaagctgac gaagtaccaa aagtattgaa cggtttaggt atcgcaatca tttcgacttc
    52261 tgaaggtgtt gtcactgata aagaagctcg tgaaaaaaat atcggcggcg aagttatcgc
    52321 ttacgtatgg taatcaagta gaatctacaa ggaggtgtct ttaagtgagc cgtatcggaa
    52381 ataaagtcgt tgttatccct gaaggagtaa cagttactca agacggaaac aacgttacag
    52441 ttaaaggacc aaaaggtgaa ttgactcgta ctttttcagc agatatcaaa atgaatatcg
    52501 aaggaaacga agtaactttc actcgtccaa acgacagtaa agaaatgaaa acaattcatg
    52561 gaacaactcg tgcgaacttc aacaacatgg ttgtaggtgt cagcgaaggt ttccaaaaag
    52621 cgttagaact aatcggggtt gggtaccgtg cacaattaca aggatcaaaa cttgtattga
    52681 acgttggtta ctcacatcca gtagaaatca caccaccagc tggagtaact gttgaagtac
    52741 cagctaacac acaagtaatt gttaaaggtg cgaacaaaga agaagttggc gaattagctg
    52801 caaacattcg tggaactcgt cctccagaac cttataaagg taaaggtatt cgttatgttg
    52861 gcgaatatgt acgccgtaaa gaaggtaaaa ctggtaaata atcgtttaga aaaattgcat
    52921 agaagccagc agcaggaaaa aggcatttgg ctgaagttta ccttcaaaaa aatacatttt
    52981 tacttgtgcc gcagcttcta tttttcgaaa cgtatttact gcttgaaata gtagtacaat
    53041 aaaaataaag aggtgacaat tgtgattaca aaaccagata agaacaaaac acgtcaaaag
    53101 agacatcgtc gtgtacgtaa caatatctct ggtactgctg agcgcccacg cttgaacgtt
    53161 tttcgttcta acaaaaacat ctacgctcaa attattgatg acgtagcggg tgtaacgcta
    53221 gcaagtgcct ctaccttgga taaagaaatt tcaggtggaa caaaaacaga agcagcacaa
    53281 gctgtcggta aactagttgc tgaacgtgca gctgaaaaag gcattaaagt agtagtcttt
    53341 gaccgtggtg gataccttta ccatggccgt gtgcaagctt tagctgaagc tgctcgcgaa
    53401 aatggactag aattttagaa gaaggaggaa taccattcat ggtttatatc gatccaaaac
    53461 atttggaatt agaagaccgc gttgttgcaa ttaaccgtgt aacaaaagtt gttaaaggtg
    53521 gacgtcgtct acgttttgct gctttagttg ttgtcggtga caaaaacgga cacgtaggat
    53581 ttggtacagg taaagctcaa gaagttcctg aagcaatccg taaagcaatt gaagatgcga
    53641 agaaaaactt agttgaagta cctatggttg gttcaacaat cccacacgaa gtaatcggtg
    53701 cttttagtgg tggtagaatc ctaatgaaac ctgctgtaga aggttctggg gttgctgctg
    53761 gtggaccagt tcgtgccgta ttagaattag ctggggtagc agatatcact tctaaatctt
    53821 taggctcaaa tacaccaatc aacgttgttc gcgcaacagt tgaaggtttg aaacaattga
    53881 aacgtgcaga agaagttgca gaacttcgcg gcaaatcagt tgaagaatta atcggataag
    53941 gaggacaaca ataatggctg aattaaaagt tactttaaaa cgcagtatta tcggacgtcc
    54001 tcaaaaccaa cgcgatacag ttaaagcgtt aggtcttaca aagattaaca gttcagtggt
    54061 taagccttct aatgaagcaa ttaaaggcat gatcaatact gtttctcacc tagtggacgt
    54121 tgaagaaatt taatttagaa aaatcataac tagaacatta aggaggtgcc aaacctatga
    54181 aacttcatga attaaaacct gctgaaggtt cacgccaagt acgtaaccgt gttggtcgtg
    54241 gtacttcatc tggtaacgga aaaactgccg gacgcggaca aaaaggacaa aaagctcgtt
    54301 caggcggcgg tgtgcgttta ggatttgaag ggggacaaac accattgttc cgtcgtttac
    54361 caaaacgagg attcacaaac gtgaaccgta aagactatgc agtcattaat cttgatgtat
    54421 tgaaccgctt tgaagacgga gctgaagtaa ctcctgttgc tttagttgaa gctggaatcg
    54481 tgaaaaacga aaaagctgga atcaaagtat tagctaatgg ggaactaaac aagaaattaa
    54541 ctgtgaaagc agcaaaattc tctaaagcag ctcaagaagc aattgaagcg gctggtggat
    54601 caatcgaggt gatctaatgt ttagactatt aaaagatgct tttaaagtaa aagacattag
    54661 atcgaaaatc ctatttactg tatttgtgtt gtttgtattt cgcctaggtg cacatattac
    54721 tgtaccaggc gtaaatgctg cttcattgca aaacctttct tctttgccat ttttcaacat
    54781 gttgaacttg gtaagtggta gtgcgatgca aaacttttcc attttctcaa tgggggtttc
    54841 accatacatt actgcttcta tcgtcattca gcttcttcaa atggacatcg tgcctcgatt
    54901 tgttgaatgg tcgaaacaag gggaagttgg tcgtaaaaaa ctgaatcaag cgactcgata
    54961 tctgacatta gtattggcat ttatccaatc tgtcggtatc actgctggat tcaatcaata
    55021 cgctcaatta ggtttcgtga ataatcctaa cgtagcaact tacgtaacga tcggtttgat
    55081 tctaacagcc ggtacgatgt ttgttacttg gctaggagaa cagatcacag aaaaaggtat
    55141 cggaaatggc gtatcaatga tcatttttgc aggtatcatt tctagattgc ctgaaagtat
    55201 cagagaaatt tatgaagatt actttataaa tattgatcct tcagacatat ggaaatctgc
    55261 tatttttgtg gccattttac tagtagcggt cttgttgatc gtgacattcg taacattttt
    55321 ccaacaagct gaacgcaaga tcccgatcca atatacgaaa cgcgtagctg gcgcgcctac
    55381 aagcagctat ctgccgttga aagtaaatgc agctggagtt attccagtta tctttgctag
    55441 ttcgtttatt gctacgccaa atgctgtttt acaagcattg agcggtagat tcggttctga
    55501 aaactggtac aaagtgatga tggagatctt taactataat acagcacctg gtgctacgat
    55561 ttatacgatt ctgatcgttg ccttcacttt cttctacgcc tttgttcaag taaaccctga
    55621 gaaattagcg gaaaacttgc aaaagcaagg aagttatata ccaagcgtac gccctggtaa
    55681 ggggactgaa gagtatgtct caagattact gatgcgttta agtacagttg gtgcagtctt
    55741 ccttggttta gtggcattgc ttccaatcat tgctcaaatg gtttgggatc ttccacaatc
    55801 aatcggatta ggcggtacaa gcttactgat cgttatcggt gtagctttag aaactgcgaa
    55861 acagttagaa ggcttaatgc tgaaacgtaa atatgttgga ttcattgagt aagaaagcgt
    55921 gttgaggttt cccctcaaca cacgttctca cacatttatt aggagggaaa acaatgaacc
    55981 tcattttaat ggggctgcca ggcgcaggga aaggaacaca agctgaaaaa atcattgatg
    56041 cttatggtat tccgcacatc tcaacaggag atatgttccg cgcagcgatg caaaacgaaa
    56101 cagctcttgg cctggaagca aagtcttata tggataaagg tgcattagtt cctgatgaag
    56161 taacaaatgg aatcgtgaaa gagcgactag ctgaaccaga tacagagaaa ggcttcttat
    56221 tagatggctt tccacgtacg ctagatcaag caaaagctct ggacgcaatg ctaaaagatt
    56281 tgaataaaaa aattgatgct gtcatcgata tccatgttgg tgaagaaatc ttggtcgaac
    56341 gtctagccgg ccgatttatc tgcagcaatt gtggagcaac ttaccataaa attttcaacc
    56401 ctacaaaagt tgaagacaca tgtgatcgtt gcggtgggca tgaattctat caaagagaag
    56461 atgataaacc tgagacggtt aaaaatcgtc tggctatcaa catcaaaaat agtgaaccaa
    56521 tcttggctta ttataaagaa caaggtttgc taaacactat tgatggtgct cgtgaaatcg
    56581 atgctgtatt tgcagatgta caaaaaatca ttgaaaaata gcacgattgc ccgtagccga
    56641 cttgcattat gcagttactc ttgtcaagtt caataaatta tgatagaata tgtcagtccg
    56701 acttctagag aatcctctag atgatccatt ttgagaattt tgaggtggga actgcagttt
    56761 ccgccatttt atcgtgtgaa aatgcgaaac aagtacaagg aggtactgtg cgtggcaaaa
    56821 gaagatatga ttgaagtcga aggtacagtc gtcgaaactt tgccgaatgc aatgtttaaa
    56881 gttgaactag aaaacggaca ccaagttctt gctactgttt caggtaaaat tcgtatgcat
    56941 tacatccgta tcttacctgg tgacaaagta acggttgaat tgtctccata cgatcttaac
    57001 cgtggccgta ttacttatcg ctttaaataa ttgtactccg taaaactaca ggaggtatta
    57061 tcatgaaagt aagaccatca gtaaaaccaa tgtgcgaaca ttgtaaagta attcgtcgta
    57121 aaggacgcgt tatggtgatt tgcccagcaa atccaaaaca taaacaacgt caaggataat
    57181 tggaggtgct ataaatatgg ctcgtattgc aggagtagat attcctcgtg ataaacgtgt
    57241 agtagtttca cttacttata tctatggtat tggtaacact actgctaaaa agatcttagc
    57301 tgatgctggt gtatctgaag atgttcgcgt tcgtgaatta acgaatgaac aaacagatgc
    57361 tatccgtgca gaagtagata aattaaaagt tgaaggggat cttcgtcgtg aagtgaactt
    57421 gaacatcaaa cgtttgatgg aaatcggttc ttaccgtgga atccgtcatc gccgtggatt
    57481 gcctgttcgt ggacaaaaca cgaaaaacaa tgcacgtact cgtaaaggcc cagctcgcgc
    57541 tatcgctggc aagaaaaaat aattcttaag tgaaggaggt taaaacttca tggcagcaaa
    57601 aaaagtgaat cgtaaacgcc gtgtgaagaa gaatatagaa gcaggtattg cacatatcca
    57661 ttctacattc aataacacta tcatcatgat cactgatact cacggaaatg cattagcgtg
    57721 gtcatcagct ggttcattag gtttcaaagg aagcaaaaaa tcaactcctt ttgccgctca
    57781 aatggcagca gaaactgcta caaaagcagc aatggaacac ggattaaaga ctgttgaagt
    57841 aacagttaaa ggacctggtt ctggacgtga agcagcaatt cgttcattac aagctacagg
    57901 tttagaagtg actgcaattc gtgacgtgac tccagttcct cataatggtg cccgccctcc
    57961 aaaacgccgt cgtgtttaat gagtgccgct cattttgagt tttatcagta acgtcattga
    58021 acaagacact tttcgttttg aaaggggtaa agataagaat gattgagttc gaaaaaccaa
    58081 gaatcgcaaa aattgatgaa gaaaaagatt atggcaagtt catcgttgaa cctcttgaaa
    58141 gagggtatgg gactacttta gggaattccc tacgtcgtat tttattatct tctttgccag
    58201 gtgctgccat cactaatatt caaatcgatg gtgtactgca tgaattctct accatcaaag
    58261 gtgttagaga agacgtcact caaatcatct tgaacattaa aggcttagcg ctgaaaatgt
    58321 atggccaaga agaaaaaacc cttgaaatcg atattaccgg acctgctaca gtaactgctg
    58381 gcgatattat cgttgatagt gatgtagaaa ttcttaacaa agatatgtac atctgtagtg
    58441 tctctgaagg agctacgttc catgctcgct taacagtgaa acctggacgt ggttatgttc
    58501 aagcagatga aaataaaaaa gaagatatgc caattggcgt tcttccagtc gattcaatct
    58561 acacacctgt tcgtcgtgtg aactatcaag ttgagaacac acgtgttgga caccgtgaag
    58621 attttgataa attgacgatg gagatttgga cagatggttc aattgaaccg ttggaagcaa
    58681 tgagtttgtc tgcgaaaatc atgactgaac atttggatat ttttgtgaac ttaactgatg
    58741 aagcaaaaaa tgctgaaatc atggtcgaaa aagaagaaac acaaaaagaa aaaatgttgg
    58801 aaatgacgat cgaagagtta gacttgtctg ttcgctctta caattgtttg aaacgtgcag
    58861 gaatcaatac tgtacaagaa cttacaaata aatctgagcc tgaaatgatc aaagtccgta
    58921 acttaggacg taaatcatta gaagaagtta aagcaaaatt gcacgactta ggtttaggtt
    58981 tacgtaaaga agattaaaac cttttacgaa ggagggaaac caacgtgagt tatcgtaaac
    59041 taggacgcac atctagccaa cgtaaagcga tgttgcgtga tttaacaact gatttattaa
    59101 tcaacgaacg tattgtgact actgaagctc gtgcgaaaga agttcgctca actgcagaaa
    59161 aaatgatcac tttaggtaaa cgcggagatt tgcatgcacg tcgtcaagct gcggctttcg
    59221 tacgtaatga agttgccagc gtgcgtgaag aaaatgagga catcgtcatt gaatcagctt
    59281 tacaaaaact gttcaacgat attgcacctc gttacgcaga acgtcaaggc ggatacacac
    59341 gcattttgaa gacagaacca agacgcggtg atggtgcacc aatggttgtc ttagaacttg
    59401 tctaaaatca cactaataac atcactttat agatgattct tttagagtgt tatgatgttg
    59461 gaagatattt ttccgagtct agctcagcgc tgctcctcaa gcgaatatcg cttggggagt
    59521 aagcactcat cataaagtgt ttttttatac cttcaattac ctgtcatgac agatatgttt
    59581 gaaggcataa aaaaacattt taagcgagaa ataattgaaa attttgtctt tttttgatag
    59641 tgttagtagg aactttagga tgaagaagga gtaatcatgg agccaattat tgaattagaa
    59701 aagataaatt ataaatacca gccagatgat cttcgccccg cattgaaaga tgtctccttt
    59761 acaattgata aaggtgagtg gatcgctatt attggacaca atggttcagg aaaatcgaca
    59821 cttgccaaaa cgatcaatgg attgcttctg ccagaatcag ggatcgtgaa agtgggaaat
    59881 caaatattgg atgaagaaaa tatttggacg atccgacaga tggttggaat ggtttttcaa
    59941 aatccagaca atcagtttgt tggttcgaca gtagaagatg acgttgcttt tggtcttgaa
    60001 aatcaaggga ttcccagaga agaaatgcta gtgcgtgtga aagatgcact tgagaaagtt
    60061 agaatggctg agtttgcttc acgcgagcct gctcgtttat caggagggca aaaacaacgt
    60121 gttgccattg ccggcgtggt tgccttacga cctgatatca ttattttaga tgaagcaacc
    60181 agtatgctag atccagaagg tcgtgaagaa gtgatctcta cgatcaagaa gatcaaagaa
    60241 gaaagtcaat tgaccgttat atctatcact catgatattg atgaagcagc taatgccaat
    60301 cggatactag tgatgagaca aggcgaactt gtccgtgaag gtacaccaaa agaaattttt
    60361 tctgctggtc ctgaactgat cgacttaggt ctagatctac catttcctga aaaactgaag
    60421 agcgcactga aagaacgtgg ggttgatgtg cccagtgaat atatgacaga agaaaggatg
    60481 gtggattggt tatggacatc cgttttgaac aagtagactt cacttatcag ccgaatacac
    60541 catttgaaca aagagcctta tttgacatca atatgacgat caaagaaaac agttatactg
    60601 cattagtagg gcataccgga agcgggaaat caaccttact tcagcatttg aatgcgcttg
    60661 ttaagccaac aagcggtaca gtccatattg gagaacgaga tattcagccg gatacggata
    60721 ataagaattt aaagcccatc cgaaaaaaag ttggcatcgt ctttcagttt ccagaagcac
    60781 agctattcga agaaacggta gcaaaagaca ttgctttcgg tcctaaaaac tttggtgtca
    60841 gcgaagaaga agcactagtc ctagcaaaag aaacattaga acaagttggg ctggatgaaa
    60901 gctatttgga acgttcgcca tttgaacttt caggagggca aatgcgtaga gtagcaatcg
    60961 ctggtgtgct tgccatgagg ccggaagtac ttgtattgga tgaaccaacg gcaggacttg
    61021 atccccaggg cagaaaagaa atgatggaga tgttttggcg tcttcataag gaacagcaga
    61081 tcactattgt attagttacg catttaatgg atgatgtagc caattttgca gattatgtct
    61141 atgtgttgga aaagggacga atcgttaata gcggggagcc gcaagaggtc tttcaaaaca
    61201 ttgaatggct aaaagaaaaa caactaggcg tacctactgc caccgagttt gcagaagaat
    61261 taatggctaa agggatgaac tttgctacct tgcctttgac agctgaagaa ttagcagacg
    61321 ctatcgtcca ttatgctgga ggaaatgccc atgatgaata aattgatatt tggtcgttat
    61381 atgcctggag attccttgat ccatcgattg gatccccgag ctaaattatt agccagtttt
    61441 tattttatcg gaatcatttt tttagcaaat aattgggaaa cgtacctttt gttggcattt
    61501 ttcacattac tttctgtctt cttgtcaaaa gtagatttga aattttttat ccgaggcatt
    61561 cgcccattga tctggctaat cctcttcact gtagctttgc aggtcttgtt tacaagtggc
    61621 ggagaaactt attggagttg gggtgttttc catatcactg aatttggtgt gatgaacggc
    61681 ctctttattt tctgtcgatt cgtgttgatc atttttatgt cgactctact cacgcttact
    61741 acaccgcctt tggaattatc ggatgcgatt gagtacttgc ttcgtccatt gaaagccgta
    61801 cggttcccag tccacgaaat ttcattgatg ctgtcgattg ctcttcgttt tgtgcctaca
    61861 ttgatggacg aaacagaaaa gatcatgaac gctcagcgcg ctcgcggtgt tgattttggt
    61921 gaaggaaacc tagttcagaa aatgaaagca gtcattcctt tgctgattcc tttatttgtc
    61981 agcagcttca accgtgcgga agatcttgcg actgctatgg aagcaagagg ctacaaaggt
    62041 ggggaaggaa gaacgaaata tcgtgtattg cattggcata atagagacac gatggttatc
    62101 attgcttttg tattattaac tgtgctactg atctttttaa gaggataaac gttcagtggg
    62161 ccgctatcta ttcttttctt gctgatctgt ttgcaagaaa ggatgcaagc agtgtatgac
    62221 aagaaatatt agggtctgtg gcagactaag gtcacaggcc tctttctttt atctgaaaag
    62281 aaagaacgaa aaaattttat agaaaaatca ttattccaga taacctttta ggaacaaagg
    62341 agagaaaaac atggtacgtt acaaagcaat cattgcgtac gacggaacaa attttaatgg
    62401 attccaaaaa cagccaaatg ggcgtactgt tcaagaagaa gtagaaaaaa cattacaaaa
    62461 aatggcaaat ggaaaagaaa tcacggtctt tggttcggga aggacagatg ccggagtaca
    62521 tgcgatcggg caagtaatcc attttgatta tccagaggag cgtccgttag aacgaatgcg
    62581 tttcgcgcta gatacccagt caccagagga tattgctgta agacaagtag aaattgtttc
    62641 agaagacttc cacgcaagat atttggtcaa agaaaagacg tatcaatttc gagtggatat
    62701 agggaaacca cgcagtcctt ttcgacgcca ttatgcaagt tattttcctt acccacttga
    62761 tttggaaaaa attcagcggg cgcttcctga tttattaggg actcacgatt tcacctcttt
    62821 ttgtgcatca ggaagctcga tagaggataa aatacgtacg atctacgagg ctaagatgga
    62881 ggtaaatgaa acaaaagacg aattgttgtt tacatttagg ggaaatggct tcttgtataa
    62941 gatgatacgg atcttagtag gaacgctttt gaaaattggc aatggccgat tgcctgaaga
    63001 tagtatccca gatattattg cgaaaaaaga tcgtaatgca gcaggaccta cagcacaccc
    63061 tgaaggactt tatttgtatg aagtgaaata tgaataagag gttatgatag aagtggtcag
    63121 cttcaagaaa taagaaggta tttacgaaaa ttacttctca aaattttgca aaatttcggc
    63181 ttattttcga agaagctact tctggaacac cgtttaagga agaggtcgtg aaatcagcgt
    63241 tttgacctga gtattagaga aacaaacggg aaaatagctc ctcatatttt gccgtttgtt
    63301 gagctaatac cgaaggtcag ctgattgaac accgtttaag aaagaggtcg tgagcctgtc
    63361 atttaactct gagtcacaaa gagcaatttc aaaaaacagc ttctcatctt tgatgaatat
    63421 cgtgacttgt ggacgaggcg ttgataagtg aatcatcttt ttgttaccta ttttttctgt
    63481 aaccaaaaaa cgatggaacg ttgttttgat tgcccttggt gcggacagtc tgaacaggtt
    63541 ccatcgtttc tttattttct acgtcttctt ctgtgtgtgt ttcgtggttg ttcttctatt
    63601 gctggatcaa tcgtgccata aaaggggcga ttgcggtcaa ggtaccagac ataaagaata
    63661 aacaatgcaa tacataggac aaaaaggatt gcacctatcg taaatccttc aggcaagtaa
    63721 ctgaaagtga cagtatgttt acctgctgga acttttatca tcaagaatgc gtctttgaaa
    63781 ttgctgacct tgactggttt cccatcaatt ttagctttcc atcctttgtc ataaggaata
    63841 gttgtcacca acatggaatc ttcagcggta tttacagtcc cagaagcttt tctgccagtg
    63901 gcttccattt cgacaccttt ttctttgatc tgattcattg cggcttgata ggctacaaca
    63961 tctagaccaa cgactttagg ctccatgaaa cttatttctt tcgtcccata aaaacttgca
    64021 gtaaatgtca ctgttgtctc ttgaggataa tagccaagat catagtattg accagaaatg
    64081 ttgatttgcg tttttcgcga tgtaccattg acagtgatcg tcgcactaga actttctaat
    64141 tgcgcaaaat tggttgggaa caaacttaag taagcttgtg tatttgcagg cacatggact
    64201 tcccaagtga gctcttttgg ctggttatct gctgtttctg agtaagtgac gccagtcgtt
    64261 gtctttgtga tcgtcgtatt caactgattc ttcaaagtag gtgtatagaa tgtaaaatac
    64321 tgttcattta gaccagacaa tgtattgatc aagtttgtct gactggtcaa attatctgtc
    64381 actggctgct caacatcatt aacggacaac ggtgcagtaa atgctagtgg caatgcattg
    64441 acattctgat acagtgtata gtcgccgctt tgaccagctt ggcggaagcc atatttactg
    64501 aacgtgcttt cgtttttaga aatgttatat ttgattcctg taaacccatc catcagcaaa
    64561 gtattattgt tataacggat attcaaattt gtccctcttg aacggaaacc taatttatcc
    64621 aggtaagagg aagaattacg atttcttata gaagaaaaca aactgatacc actgtaacca
    64681 tagttgatcg aatcgtttgc agatactgga tccagatttt ccaagcggta aaacgagtcg
    64741 gaatcttttt ttgtttcatt tacaagagct ttcaagtcag gataaggatc agtatataaa
    64801 ctgcgggaag cataattcca atcatcaaga atgccatgga tcatactgtt cgtattgacc
    64861 agtgcttcag cagacatgat caataataac aatatactca gatgattgaa tgaaagtttt
    64921 ttatgctgat aaaatcccac agcaaaagca taaatcccta agaaaaccac tgtcaataca
    64981 aaggaagtca tcgtcacata ggtgtagctt ccttcaggtt tgatgcccca tgccaccgca
    65041 aagagagcag ctacgaccaa aatgattcct aacaacagcc cacgatcgtc ttgttctatc
    65101 ttttcccatc cttttcctgc aagcaggagc actaagaaag agaacagata ggcataacgg
    65161 aataaaaaca tattcggtgc atgcatacca tgccagaaca aattcaatgg aacaagataa
    65221 aaacttgcga taagtatggc gaaaagactg ccgtacaaaa atttattttt caacggtact
    65281 ttccgactgg caaaataaaa cagacagagg actaatggta gcagacctgc gtaaacgaac
    65341 ggaatcgatc cgtattttgt cgtgtcataa acaccaatca tatttttcat gattagatcc
    65401 agataagaag tcgcttctgt tttcaacgta gtgatctgac tcagtgcctc gccatttgac
    65461 cgaagatcca gcactgctgg caatacaatg atcattgaag cgccgcctgc cagtaaagaa
    65521 gtgataccat aaggaacaat cgttttttta tattctgacc agtttgtcaa tagacgggcg
    65581 ataaaatata agaaagaaaa tacgccaatc ataaaaccca tataaaaact ggataaaaac
    65641 aagagtaaat aactgataaa taaaagtttc ggccgtttaa gatccataac acgatgaatc
    65701 cccaaaatca ctaatggaag ataaaaaaat gcatccagcc acatgatgat ctccgaatgt
    65761 gcagtgataa atgagatcaa tgcgtaactg acgctaagtg caacatgccc ccagcgagga
    65821 atcttgaacg tacgtgcaag aaaccaaaaa gccaatccgg cacagccgac cttaagcaaa
    65881 gtcagaacat acagtgcatc tggcatcatc tggtttggaa aaaagaacac cagtggcgtg
    65941 aagatcccgc ctagatagta agaaattaaa gataaataat tcaatcctag agaagcattc
    66001 catgtataaa aaatactttg ctttccaaga agaacatttc ggaagctggc atgaaaatta
    66061 gaaaactgtg aaaaggcatc acttgccaaa atacttctgc tgcttccagg atagattccg
    66121 atacttagat aaacaagtgc taaaatcaaa aacggaatca ggaaactagc tg
//
LOCUS       DAZKKW010000005        62581 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.005, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000005 DAZKKW010000000
VERSION     DAZKKW010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 62581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 62581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..62581
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.005"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..117
                     /locus_tag="ACG8PO_000273"
     CDS             <1..117
                     /locus_tag="ACG8PO_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HFQ0205632.1"
                     /translation="SRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            293..1036
                     /locus_tag="ACG8PO_000274"
     CDS             293..1036
                     /locus_tag="ACG8PO_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0205633.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            1029..2609
                     /locus_tag="ACG8PO_000275"
     CDS             1029..2609
                     /locus_tag="ACG8PO_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HFQ0205634.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(2860..3339)
                     /locus_tag="ACG8PO_000276"
     CDS             complement(2860..3339)
                     /locus_tag="ACG8PO_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFQ0205635.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(3355..4107)
                     /locus_tag="ACG8PO_000277"
     CDS             complement(3355..4107)
                     /locus_tag="ACG8PO_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFQ0205636.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            4224..4294
                     /locus_tag="ACG8PO_000278"
     tRNA            4224..4294
                     /locus_tag="ACG8PO_000278"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4256..4258,aa:Gly,seq:ccc)
     gene            4660..5385
                     /locus_tag="ACG8PO_000279"
     CDS             4660..5385
                     /locus_tag="ACG8PO_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HFQ0205637.1"
                     /translation="MSKKTTKAIVGIVAAVIAGALGISTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            5639..5905
                     /locus_tag="ACG8PO_000280"
     CDS             5639..5905
                     /locus_tag="ACG8PO_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HFQ0205638.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            5902..6333
                     /gene="ruvX"
                     /locus_tag="ACG8PO_000281"
     CDS             5902..6333
                     /gene="ruvX"
                     /locus_tag="ACG8PO_000281"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFQ0205639.1"
                     /translation="MRVMGLDVGSKTVGVAVSDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            6359..6661
                     /locus_tag="ACG8PO_000282"
     CDS             6359..6661
                     /locus_tag="ACG8PO_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HFQ0205640.1"
                     /translation="MTEHNHSHEHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEE"
     gene            6851..7687
                     /locus_tag="ACG8PO_000283"
     CDS             6851..7687
                     /locus_tag="ACG8PO_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFQ0205641.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQAIVIVVLLINLYNAFVILQTLEGSGFKQVKITEKSF
                     FK"
     gene            7751..8182
                     /locus_tag="ACG8PO_000284"
     CDS             7751..8182
                     /locus_tag="ACG8PO_000284"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0205642.1"
                     /translation="MLREMRLGDAEALREINAEQLGYDVPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKHAEKRGIGKALMTGVEEA
                     KRRGISAIRLNSAEYRVEAHRFYERIGYHSDKMQKRFLKIL"
     gene            complement(8244..9566)
                     /locus_tag="ACG8PO_000285"
     CDS             complement(8244..9566)
                     /locus_tag="ACG8PO_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HFQ0205643.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFLDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRNVKPSIPQLGVGLMVSL
                     TFFILGQICNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(9720..11360)
                     /locus_tag="ACG8PO_000286"
     CDS             complement(9720..11360)
                     /locus_tag="ACG8PO_000286"
                     /EC_number="4.1.1.101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043883 - malolactic enzyme activity
                     [Evidence IEA];
                     GO_process: GO:0043464 - malolactic fermentation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malolactic enzyme"
                     /protein_id="HFQ0205644.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAVFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAATILEKYEDKITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQTILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     KGLTPGQIPFARKRSEFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQKLNREEITDIKEAVESAKWVPEYKSLED"
     gene            complement(11801..13120)
                     /locus_tag="ACG8PO_000287"
     CDS             complement(11801..13120)
                     /locus_tag="ACG8PO_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element IS1678 family transposase"
                     /protein_id="HFQ0205645.1"
                     /translation="MTSLHKNQVKFNSNITISHTGGQLSSDSGLVLVKELMNIFGFSE
                     LAKQHIHIEDERAYFTHDNLSILEQFIMQLIAGYSADSAANLLRQDPVFKAVLDRKEL
                     ASQSSLSRFLDRLSEENIHELQALNQELIDKARLIRNDTELIIDLDSTHSDTFGHQEQ
                     TNYNTHYQTYGYHPLVAFDGLTGDFLKAELRSGNQYTSKGVKEFLTPLLEHYNHSLPN
                     TDILVRGDSGFATPGVYDSCESKKSHYVIRLKNNRRLGQIAEKSVLYGDNQKWEEREV
                     QYFSTTYQAQSWSQSRRVCIRSTREAGELLFRHEFIVTNLSENVSPDTIFSIYAKRGT
                     MENFIKEAKAGFYFDKTDSPRFLENHVRMMLSLIAYNLVNFLRTIGFEEVQKGMTIHS
                     IRLKFLKVAGKLVQTGRRVYLKLSSYHVYQNEFYRVFARLRRASQWI"
     gene            13377..13847
                     /locus_tag="ACG8PO_000288"
     CDS             13377..13847
                     /locus_tag="ACG8PO_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HFQ0205646.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLGANKAIISELTV
                     GGLVDSLALAEGFIGEDIYEMFSEIRQLKKGENCIEVHHESPNVRFKRGDNEMIFNNH
                     VSDHTELYGIVNQILTELFGLGPYAFKEENGNPKLNEEWQETIEIQRNNPHLFQ"
     gene            14069..15172
                     /locus_tag="ACG8PO_000289"
     CDS             14069..15172
                     /locus_tag="ACG8PO_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HFQ0205647.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHQLIIPHVLRDVELPDTTTHFDKETLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDDGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            15393..16352
                     /locus_tag="ACG8PO_000290"
     CDS             15393..16352
                     /locus_tag="ACG8PO_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFQ0205648.1"
                     /translation="MTYNHLTPTELVMIEAYFNQSQPVSKVANLLQRSRQTIYKVYRF
                     LKSGGSAMSYYKQYKKNKRNCGRHPIVLPDEQQEYIQKKVVQGWAPDVLIGRAEFPIA
                     CSVRTLYRMFKDKTFDTHDLPMKGKRKPNGHKEKRGKQGFRRSIRDRKTDYTQFDHEF
                     GHLEGDTIVGGKHKSAVITLVERLSKVIITLKPEGRQAKDIENRLNQWFRCVPKNLFK
                     SITFDCGKEFSNWKKISNTNDISIYFADPGTPSQRGLNEHSNGLLRKDGLYKAMDFNG
                     VSETFIQSVASKRNHIPRKSLHYRTPMEVFLSYVTKDDLSNLI"
     gene            16522..17202
                     /locus_tag="ACG8PO_000291"
     CDS             16522..17202
                     /locus_tag="ACG8PO_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFQ0205649.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGPLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTFTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            17292..18326
                     /locus_tag="ACG8PO_000292"
     CDS             17292..18326
                     /locus_tag="ACG8PO_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HFQ0205650.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTVPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNAFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            18340..18675
                     /locus_tag="ACG8PO_000293"
     CDS             18340..18675
                     /locus_tag="ACG8PO_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0205651.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPIPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEQKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            18659..21139
                     /locus_tag="ACG8PO_000294"
     CDS             18659..21139
                     /locus_tag="ACG8PO_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0205652.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLTDIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISPSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSLQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYESKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            21195..22754
                     /locus_tag="ACG8PO_000295"
     CDS             21195..22754
                     /locus_tag="ACG8PO_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0205653.1"
                     /translation="MRRKLLLLKSEMLEGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     VSVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQIRYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFAITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            22751..24115
                     /locus_tag="ACG8PO_000296"
     CDS             22751..24115
                     /locus_tag="ACG8PO_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HFQ0205654.1"
                     /translation="MKIVIIGASHEGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQTIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEYTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            24286..25356
                     /locus_tag="ACG8PO_000297"
     CDS             24286..25356
                     /locus_tag="ACG8PO_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0205655.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAIGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            25371..26039
                     /locus_tag="ACG8PO_000298"
     CDS             25371..26039
                     /locus_tag="ACG8PO_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0205656.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEANELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            26059..26637
                     /locus_tag="ACG8PO_000299"
     CDS             26059..26637
                     /locus_tag="ACG8PO_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0205657.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            27038..27949
                     /locus_tag="ACG8PO_000300"
     CDS             27038..27949
                     /locus_tag="ACG8PO_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFQ0205658.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(28327..29244)
                     /gene="rnhC"
                     /locus_tag="ACG8PO_000301"
     CDS             complement(28327..29244)
                     /gene="rnhC"
                     /locus_tag="ACG8PO_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HFQ0205659.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELDKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            29446..29883
                     /gene="zapA"
                     /locus_tag="ACG8PO_000302"
     CDS             29446..29883
                     /gene="zapA"
                     /locus_tag="ACG8PO_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFQ0205660.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            29941..30489
                     /locus_tag="ACG8PO_000303"
     CDS             29941..30489
                     /locus_tag="ACG8PO_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HFQ0205661.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            30604..32964
                     /locus_tag="ACG8PO_000304"
     CDS             30604..32964
                     /locus_tag="ACG8PO_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HFQ0205662.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKIENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNTYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            33051..33365
                     /gene="trxA"
                     /locus_tag="ACG8PO_000305"
     CDS             33051..33365
                     /gene="trxA"
                     /locus_tag="ACG8PO_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HFQ0205663.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            33547..35325
                     /gene="uvrC"
                     /locus_tag="ACG8PO_000306"
     CDS             33547..35325
                     /gene="uvrC"
                     /locus_tag="ACG8PO_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFQ0205664.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKNFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELASKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMVKNDKHKTNELLFGTDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            35470..36369
                     /locus_tag="ACG8PO_000307"
     CDS             35470..36369
                     /locus_tag="ACG8PO_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0205665.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            36362..37624
                     /locus_tag="ACG8PO_000308"
     CDS             36362..37624
                     /locus_tag="ACG8PO_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0205666.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPIRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            37785..39683
                     /locus_tag="ACG8PO_000309"
     CDS             37785..39683
                     /locus_tag="ACG8PO_000309"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HFQ0205667.1"
                     /translation="MDRYLDLLSEKFPSTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETDISQFTIFTAYPEYALTTDWYKEQDKS
                     QLIHHLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFAP
                     KNSKNLSEREQEELEKIHQALSILQFKSEHQLLDRRPEFKMDDRKLLEKIDYEASTID
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTAKEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDTKEEGSAIINGHTPVKVKKGESPIKANGKVFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRDTTKGMELQQQITQLKR
                     LLDYRKDD"
     gene            complement(40013..40732)
                     /locus_tag="ACG8PO_000310"
     CDS             complement(40013..40732)
                     /locus_tag="ACG8PO_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HFQ0205668.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIGQIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFTTHEVKIVSD
                     SLLSNLLPDPYFVNSYHHQAVKDLSPLLKAIAFSNDGLVEAVQSKDDMHKILGVQWHP
                     ELTHRVHPSEQRLFDFFVNEF"
     misc_feature    40922..40969
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF03071"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="sodium ion sensor (DUF1646 type); this
                     cis-regulatory element may regulate processes involved in
                     with the transportation of sodium ions; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF03071"
     gene            41069..41401
                     /locus_tag="ACG8PO_000311"
     CDS             41069..41401
                     /locus_tag="ACG8PO_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0205669.1"
                     /translation="MKQVLEALEQIRKAEEENEKRAQTLRQQLKIYATEKEQELETIQ
                     TNYRQQLKQLVEEKEQFEIQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            41388..43391
                     /locus_tag="ACG8PO_000312"
     CDS             41388..43391
                     /locus_tag="ACG8PO_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HFQ0205670.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFINKLKEVLDLKEKWEQLTEERDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAVIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKIKERFIHATYLIVLQGWVDAEEKQNLLHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLVEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLLVLPRGMKRFVKFFEILSIPSIIWGLIYSSFFGQSLPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALVGAMLIKNTAFVYLGAGLSIIAALCILIVPIIQTPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            43410..43880
                     /locus_tag="ACG8PO_000313"
     CDS             43410..43880
                     /locus_tag="ACG8PO_000313"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HFQ0205671.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            43901..44485
                     /locus_tag="ACG8PO_000314"
     CDS             43901..44485
                     /locus_tag="ACG8PO_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HFQ0205672.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQVETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAVTEMENWDESEQ
                     IQFIKNALYSLPLTGKVAFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            44500..45486
                     /locus_tag="ACG8PO_000315"
     CDS             44500..45486
                     /locus_tag="ACG8PO_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HFQ0205673.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNIDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFESETIRKRVRQVYDA"
     gene            45476..45787
                     /locus_tag="ACG8PO_000316"
     CDS             45476..45787
                     /locus_tag="ACG8PO_000316"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HFQ0205674.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            45809..47590
                     /locus_tag="ACG8PO_000317"
     CDS             45809..47590
                     /locus_tag="ACG8PO_000317"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HFQ0205675.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            47583..48959
                     /locus_tag="ACG8PO_000318"
     CDS             47583..48959
                     /locus_tag="ACG8PO_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HFQ0205676.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            48966..49601
                     /locus_tag="ACG8PO_000319"
     CDS             48966..49601
                     /locus_tag="ACG8PO_000319"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HFQ0205677.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            49631..50986
                     /locus_tag="ACG8PO_000320"
     CDS             49631..50986
                     /locus_tag="ACG8PO_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFQ0205678.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(51178..52572)
                     /locus_tag="ACG8PO_000321"
     CDS             complement(51178..52572)
                     /locus_tag="ACG8PO_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFQ0205679.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            52664..53635
                     /locus_tag="ACG8PO_000322"
     CDS             52664..53635
                     /locus_tag="ACG8PO_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HFQ0205680.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            53632..54414
                     /locus_tag="ACG8PO_000323"
     CDS             53632..54414
                     /locus_tag="ACG8PO_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HFQ0205681.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(54583..54954)
                     /locus_tag="ACG8PO_000324"
     CDS             complement(54583..54954)
                     /locus_tag="ACG8PO_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFQ0205682.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            55088..55531
                     /locus_tag="ACG8PO_000325"
     CDS             55088..55531
                     /locus_tag="ACG8PO_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFQ0205683.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(55574..56383)
                     /gene="yidA"
                     /locus_tag="ACG8PO_000326"
     CDS             complement(55574..56383)
                     /gene="yidA"
                     /locus_tag="ACG8PO_000326"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HFQ0205684.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(56402..57769)
                     /locus_tag="ACG8PO_000327"
     CDS             complement(56402..57769)
                     /locus_tag="ACG8PO_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFQ0205685.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(57753..58583)
                     /locus_tag="ACG8PO_000328"
     CDS             complement(57753..58583)
                     /locus_tag="ACG8PO_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HFQ0205686.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLEDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            58746..59183
                     /locus_tag="ACG8PO_000329"
     CDS             58746..59183
                     /locus_tag="ACG8PO_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HFQ0205687.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTAGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            59259..59879
                     /gene="rpoE"
                     /locus_tag="ACG8PO_000330"
     CDS             59259..59879
                     /gene="rpoE"
                     /locus_tag="ACG8PO_000330"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HFQ0205688.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            60068..60574
                     /locus_tag="ACG8PO_000331"
     CDS             60068..60574
                     /locus_tag="ACG8PO_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HFQ0205689.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            60591..61490
                     /locus_tag="ACG8PO_000332"
     CDS             60591..61490
                     /locus_tag="ACG8PO_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsiV family protein"
                     /protein_id="HFQ0205690.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            61577..62461
                     /locus_tag="ACG8PO_000333"
     CDS             61577..62461
                     /locus_tag="ACG8PO_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFQ0205691.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
BASE COUNT        20511 a        10860 c        13072 g        18138 t
ORIGIN      
        1 agcagagtaa acgaaatagt aacatttgca aatggagaac aactagagat gggaaagaga
       61 agtattgata aactaaagaa ttttatgcaa gaaaagatag aaaatgaatc attttaaaga
      121 aaacaaagac tcatatctta cctgttagaa gagtgttttc ctgtagaaga gaacaaccca
      181 agtagcagcc tagtcgtttc tatccttttt tgttatgcac tctttttgac atttactcca
      241 ccaataacga agggtacaat aaaaagtaat aacaaacaaa aggagtacag aaatgattga
      301 attcatcaat gtaagtaaaa gttacgggaa aaaacaagca ttaaaagact tgtctctctc
      361 gataagacaa ggggaaattt ttggattttt aggacataat ggtgccggaa aatcaactac
      421 gattaaaagt ttagttagta ttattgaacc atcatcggga acgattttag tagatggaat
      481 gaaattgacg gaaaatcgat tgagtatcaa acaaaaaatc ggttatgtgc ctgattcccc
      541 agatattttt ttacaattga cggctggaga atattgggac ttgatttcag ctgcctatga
      601 attagacaca caaaaaaaag aaaagcgttt agcagaattg acagcgcttt ttgatatgta
      661 tagtcatcaa aatgaaacaa tcgcaagttt ttcacatgga atgcgtcaaa aaacgatttt
      721 gatcggcacg ttgctgccag atccagatat ttgggtattg gatgaacctt tgcaaggact
      781 tgatcctcaa gcagcatttg atttgaaaga aatgatgaag gctcatgccg caaaaggaaa
      841 aacagtcatt ttttctacac atgtattgga taccgcacaa cagttatgcg ataaactggc
      901 tattttgaag aaaggtgaat tgatttatca gggggcagtt agtgaattat tgaatggctc
      961 acctgatgaa acgctagaaa agatttattt gaaaatggct ggaaggcaag cgacaggaga
     1021 tggaccgtat gtataagcga caattattag aattaattcg agttaatctt ttatatgtta
     1081 atccacagct cacgaataaa gcaagagaga aaggaaaaaa gggaaagcga ttaactatct
     1141 atcttttgtc tcaaaatctt ttttcagggc tgattttttt actgatttac ggatttgcta
     1201 tgtttgctat gaactttgtt aaattgccag gtttctttac ttattacgtg gcactttttg
     1261 gtatgttagg tttaactcaa agtatatcag tcatttataa tatctttttt gaaggaaatg
     1321 acctgcaagt atttttgcct ctgccttttg gacaagaaca ggttttttca gcaaagattt
     1381 tggtagttgc tttgaccatc attccttttg tttttccgct tctagtcatg tttattttaa
     1441 ccggatggcg agctgatcta gggattttgc ttactatcat tatctcaatc cttttgtttt
     1501 ctctattttt gattattctt ttttgtctgg ctagtttgat tgtttttggt ttggctcgaa
     1561 cagcagtgtt taaaaaacat agaaaaacag tttctagtat cttgcttggc agctcgatgc
     1621 tgatcgcatt tgctggaatc atgtggatga atcatcaaac ggggactatt gaaaatgagt
     1681 taacagatcg gcatccgatt tcgatattat tgccattcta tgatgctgcc agtcagccac
     1741 tttctcaaca aggtttattg ggttggggaa tactagctat cacggctctg ggatttttcc
     1801 ttgctgtaaa acagctgata gtaccaaagc ttacagagca actgactgat tcaaacagtg
     1861 agttcaaaat aaaaagaacc cataaaaaga accaaaatgt gcatcagctt ttatttagtt
     1921 acaatgctca attagtaaaa gaacctaatt tgattatgca agttctttct tcttccttgt
     1981 taactccaat catttttatt cttgcttttg ccctaggagg cgaaataaaa ttaactgatt
     2041 tagataataa gttaatcggt gttgttttcc tggtagggat cgcattggct ttcttaacag
     2101 ttaatcagac ttcttttatc agcaatctta tttctttgga tcaagaaaat tttctgttta
     2161 tccgttcttt gccaatctct atgaaccaat atttaaaaga aaagttcaga ttcggatgga
     2221 tattgcagag ttttttaact ggtgggatcg cattattaag cggactgtct tttcagctgc
     2281 ctttgttttt tatccctagc ttgctatttg gttctttatt cggctgctac ttattgtcac
     2341 tacgttattt cgctagagat tatcggttgc tgctgctgaa ctggaccaat attaaccaat
     2401 tatttaacag aggttcagga agtttgggtt tagtcgcaac tatgatgggt tcattgatca
     2461 tcagtattat tcttatcgtc ttatacggaa tgttggcttt gttttatcca ttttggttag
     2521 tgaatattcc tgtgatcggt ttagttctaa tactttccgc tctatggatc gtttattatc
     2581 aacgaacgtt ttggaaaaaa tttgaataaa gaagaggggc cgtgacaaaa atcatgtcgc
     2641 agcccctttt ttacagatag agggtgttca aaagtcagtt tctcagtatt aagtcaattt
     2701 tatgtaaaaa tgtgagaagc tatttttaca aaactttttt taatactcat aattacttct
     2761 tattgctcct gcggttactt gtcgcaatgt ttgtgagata ttttgtcatc gctcaaggct
     2821 gaacgtctct ttcacaacct tttctttgtt acacttcgtt tatttaaaaa gccgtttttt
     2881 tcgtttcatc atgattgcgt cacgctttac tttgaaatta cggatgttat ggttaaagac
     2941 ttctgataat acaagaccta aagcgattgc tccagcaatc ataattgctt ggaccgcata
     3001 atgagccgct tctcgatagg attcagtaac gagatttcga accgcttgat aagctaaacc
     3061 accagggact agaggaacca tcccgggaat attgaaaatc gttacgggca ttttcaaacg
     3121 tttagagaaa agatcgctga tgaatgctac tcctaaagaa ccaagcaatg aaccaaaagc
     3181 agcagatccg cctatttgaa cacagatcca gtagatcatc cagccaaaag caccagaaag
     3241 accacaacaa atcaatgaac gcctaggtac atttgtaata acggcaaatg ccgcagtcgc
     3301 taaaaaacta aaagaaaact gaataaatat cgtccccata cttgtgttcc tttcctaata
     3361 aaaaagctgg aaaataaaag caatcgcaaa accgatcatc gtagcagtca tcatcgcttc
     3421 tgttccgcga gaaactcctg agacataatg tccagctagc aaatcacgga tagcgttcgt
     3481 aatttgaact ccaggaacta aaggcatcac acatccgata atgattagat cctgattgct
     3541 tcctaagcca aaacgtacgc tgtagatagc ggctatgcca atcaataaag aagataaaaa
     3601 ttccgataaa aatttgattc taaaaaattt caaactatat agataagctg cgtatcccaa
     3661 tcctccaatg actaaagtca caggtaaatc ggtcatcttt ccgccaaata acaccataat
     3721 tgttccactg atcactgccg cgctaacgat ttgcaaccag acaggaaaga aagaacggtc
     3781 tcggtcgatt gcttgcagtt cgcagtaaag ttcttctaat gttagctctg aagcaacata
     3841 tttacgtgaa agatcattta cttttactac tttttctaaa ttaatcgaac ggttggtaat
     3901 ttgttccatt cggatggtcg aggtacgatc aagaccaata aagagaccgg tttgagtgac
     3961 atagctgacc aaacggaaat ttccagaagc taaagcaatt cgactcatcg tatcttctac
     4021 ccgatacatc tccgcattgc tttccatcat gattttccca gcgagtaaac aagtgtccag
     4081 caacagatct aattcttttc ccgtcatagc atcttctcca ataattgatt gattagtttt
     4141 attttactac aaaacatatc ggaagcgtaa agaataaaaa aacttgagga aggagtgaaa
     4201 gaggcgtata ctgtttttgt atcgcgggtg tagtttagtg gtaaaatccc agcttcccaa
     4261 gctgatgtcg cgggttcgat tcccgtcacc cgcttaaaag aggctgtgaa aaagctgccc
     4321 tgcatcaagt aataagaata gtcaagcaaa aaagcttctt atgtttttga cttggttgga
     4381 cttattaccg cagatgcaag cttttgaaca ctgtttacca agaggtcgta cagaagccgt
     4441 ttagttttga gtattaagaa aaattttttg aatgataata aataccttgt aaatagtgtt
     4501 ttgtgattga gtagagacga tgaatccgtc tctttttttg tattcttaag ccgtctaaaa
     4561 actggtttta tttttttact gtttctattt ctttccagta tttatttata ggggaacata
     4621 tgttaagatt tagggaactg ttagtaaagg agtaaagaaa tgagcaaaaa aacaaccaag
     4681 gcgatcgtgg ggatcgtcgc tgctgtgatt gcaggggcac tcggtatttc caccacacaa
     4741 aaagattcag atatcttcca acaaattaca accatttttg attccacacc gcaagcttct
     4801 caagtagcgg atgtgccaga atatgatgga aaacatcaag aaatagaaat caatgggaat
     4861 cagccggagt ttacaaaaga ggatcttagt ctttccaaag ggacatggga aagctattca
     4921 aatatagaca aattgaacag agtaggacaa gccaatgcga tgttgggaaa agacttgttt
     4981 cctactgaaa agagagaacc tttgtatatt gatccgacag gttggaaaca aaagaaatta
     5041 agtgacggac aatggttata caaccgcagc catttgattg gtttccagtt gactggtcaa
     5101 aataataaca ttaaaaactt aatgacaggg acacgttcgt tgaatgctcc gtacatgctt
     5161 gcccacgaaa atgatattgc ggcatatatc aaggaaacaa atcatcatgt acgctatcgt
     5221 gtcactcctc atttcgaggg aaatgaactg gtagcaagag gtgtgcagtt ggaagcagaa
     5281 agcattgaag acaaaaaaat tgaattcaat gtctttatct acaatgttca agatggctac
     5341 accatcaatt atgagacagg gcaagcaacg aaaacgaaaa attagtagca gcaattcgaa
     5401 ttttttatga actttatgaa aaaattgtgt aaaaacatac tttcaaagaa atatagcgta
     5461 tactttatgg aaagagggct tttgaaaaag ccacagaaaa aaggaaaaat cagacaaatc
     5521 gtgtttctta atttattcag aagtttggaa tatttgtttg tagttttccc tttcttcgag
     5581 taaaataagg gtgtagaagg taagctggaa ttaccttgcg ggaaagaggt gtagaatcat
     5641 gggttttaca gatgaaaccg tacgttttgg ttttgacgat agtcgcaaaa aagaagtgag
     5701 cgaaacatta gcgattgttt atcgggcttt ggaagaaaaa ggctacaatc caatcaatca
     5761 gattgtcggc tacttgcttt ctggagaccc ggcctacatt cctcgttatc gtgatgcacg
     5821 aaatttgatt cgccgtcacg aacgcgatga aatcatggaa gtaattgtca aagactacct
     5881 ctcaaatcat ggagtgaatt tatgagagta atgggcttgg atgtcggttc taagacagta
     5941 ggcgttgctg tgagcgatcc ctttggctgg acagctcaag gagtagaaat cattcgaatc
     6001 aatgaggcag aagaagagtt tggttttgat cggttgaagg aattagtggc gcagtacgaa
     6061 gtagaccgtt ttgtagttgg attacctaag aatatgaaca attccatcgg accaagggca
     6121 gaagcttcca tgtcatatgg agagaagatc aaagaactat tctccttgcc ggttgattat
     6181 caagatgaac gactaaccac tgttcaggca gaacgtatgc tagtagagca ggcaaatact
     6241 tcaagagcga aacgaaaaaa agtcatcgat aaattagcag ctgtgatgat tttacaaaat
     6301 tatttagacg gctcaggtaa gcaattattt taataataaa aagaaaagag gtaatcttat
     6361 gacagaacat aaccattccc acgaacacga aggacacgaa cacatcacat tggtggatga
     6421 ccaaggaaat gaaacattat acgaaatctt gctaacagtt gacggacaag aagaatttgg
     6481 acgtaactat gttcttttat atccagctgg tgtttcagaa gatgaagatg tagaattaca
     6541 agcttatgca tacatcgaaa acgaagatgg aacagaaggc gagttagaac aaatcgaaac
     6601 agacgcagaa tgggatatga tcgaagaagt attcaataca ttcatggcgg aagaagaata
     6661 gaacaaaaga atcgataagg tgtaagaaaa gctggactct ctgaaaagta gagcccggtt
     6721 ttttcgttta gcactaaatt tccttaaaaa gtagaaaagc tttcgaagcc tcgtttgttt
     6781 cttttactgt ataataaaag aaaagagtaa caatcgctaa aagatataaa caagagaaag
     6841 gaaatacaag atgattggtc tgatttattt tgtggtgatc gttctggcga acacagttgg
     6901 cgctatttcc gggatgggtg gaggcgtgtt gatcaaaccg attcttgatc ttatcggcgc
     6961 ccattctgtt gcagggattt ctttttactc aacagttgcg gtattcacga tgtccattgt
     7021 ttcgactgtg cgacaagtat caagcgggaa atcactgaat tggcagattg tcggttgggt
     7081 atctagtgga gctgttgttg gaggagtagt cggcaacatt gtattcgaag tttttttaca
     7141 gttatttgaa aacgagaagc atgtgcagct gatccaaatt tttttgacgg tattgacctt
     7201 ggtttttgcg ttcttctaca ccaagcatca tcagccaaaa tttcatttga cgtcatggac
     7261 ttggtacctt atatgtgggg gggttctggg ctttttagct agttttttag gtattggtgg
     7321 tggaccagtc aatgtttctt tgttgatgct gatgtttgca ttgccaataa aagaagcaac
     7381 actatattct ctatctacga ttttcttttc tcaactggca aaattggtaa cgattgcttt
     7441 aacaagtagc tttatgcgct ttgatttatc tatgttgttt tatgttatcc ccgctgctgt
     7501 tattggaggt ttgtggggag caagatttag cagggttttg tctccaaaaa aagtgacctt
     7561 tatttttcaa gcgattgtca tcgtggttct tttgatcaat ctttacaatg ctttcgtcat
     7621 cttgcaaaca ttagaaggat ctggattcaa gcaagtcaaa atcactgaaa agtctttttt
     7681 taagtgaaaa aaggagtata ctgtgataaa aaaattcatt taaaaaaata aaaattggct
     7741 ggtgatttgg atgttgcggg aaatgcgttt aggagatgca gaagcactac gagaaatcaa
     7801 tgcggaacaa ttaggatatg atgtaccatt aaccgttacg gtgagacaaa tgaagaaact
     7861 gttgaaggag ccagaaaaaa attttttctt ggtctatgaa gatcctactt ctaaaaaagc
     7921 agctggctat gtacaagcgg agtcctatga atcgattttc agcgatccga tgttcaatat
     7981 tatggcactt gctgtgtcta aacatgctga aaaaagagga atcggaaagg ccttgatgac
     8041 tggcgtagaa gaagcgaaaa gacgaggaat atcagcaata agacttaatt ctgcagaata
     8101 cagagtagaa gcacaccgat tttatgaacg catcggttat cattccgata aaatgcaaaa
     8161 aagatttttg aaaatcctgt aaatttttat caaaaaatac ctggtcatca agccgatact
     8221 tgatgatcag gtattttttt tgactaagaa agtaattgca tcaagaaacc tgagatgatc
     8281 aaaacgatgg caccaccaag ccgattgccc atttgagcaa aagcaataag ttccatccga
     8341 ttagctgcag ataagacagc tacatttcct gtaccaccca tcgagttgtt gcataagcca
     8401 gcagtaatag ttgattcgat tggatataaa ccaaacaaac gaccgacaaa tcctgaaact
     8461 actgaaatcg cgataacact tgtcaaacat aagacaacaa attgccatgt caaagcagaa
     8521 gccaatacat ttagattcaa taacgcaata ccgataccgg caagaacagc tgctgtcaag
     8581 tttttgacaa tcaagttgtt gaacatgata gctgcatctt cgtaatattc tggtaatagg
     8641 tttgcgattt tacagataac aacgataatg atcatgaatg cgtaagcatg gactttagga
     8701 acaaaatagt tacagatttg tcctaagatg aagaaagtca atgataccat caaaccgaca
     8761 cctaattgtg gaatggaagg ttttacattt ctaggttttc catcgccaag gtcgcctttt
     8821 tccattaatt taccattacc gttgaatttt ggcattgatt gtcccgcacg agcaactaac
     8881 gctgcaccaa tgatagctag gacatttgtc attgctgtcg caggaatcag gcgggaaatg
     8941 atagaagaag cgtccgtacc taaaacattt gcataaatcg ttgataaagg tacagctccg
     9001 gctccaacac caccagccat tgcgggcaat gagatatata aaacggaatt tgcaaaacca
     9061 tttccgataa gcatacctac gagtcctacg gcaaaaaagg agacgaccat tgaaataagt
     9121 gcaacgggaa taaaacgaac agaagctttc atcaatagat tacggttcat accaaggatg
     9181 ctaccagtaa tcaaggcagc aatataaaag tctaaaaagc ccatattgct gacaaagttt
     9241 tctgtggatt ttactacata ctcaggtaag atcccaaatg tagcaatcgc ggcagaggca
     9301 aagatcgcaa aaacggagcc accacctaaa taacttttga ctatagggat ttttgttccg
     9361 ataaaatgaa ataaattacc gaaaatgacc aaaacagcga ttgggccaat catattcgtc
     9421 ggtaattttc ctaaagccat tgcaatgatc aggacaagga ccatgatcaa ataaaggggc
     9481 aaacttacgc cggtaatttt tgttgaccaa aacccttctt ttttcttttc ctcaactgca
     9541 gttgcggttg ttgttttttg tgccattttt acagatcctt tcttattctg tatcgtatga
     9601 aaaaagccaa agagaagcgg caaaagagca gtagttggct ggctgctgtt ttgccgcagt
     9661 cgcttttttc tttttagacg tcatacaaag tttctgtatg ttcttttact ataacaatct
     9721 taatcttcta aagatttata ttcaggaacc catttagcag attcaacagc ttctttgata
     9781 tccgtgattt cttcacgatt caatttttgt gcaacaactg attttgcgac agtttcagca
     9841 atcgtttgag agaattcagt gatttttgct actggaggca agatagctgc tcctggtttt
     9901 gttacatcaa cgattccgcc taatgctcga ctagcttgag aaatgatttc agcatttact
     9961 cgtgtagctg ttgaagcaat caaaccaagc cctaaacctg ggtacatcaa cgcattattt
    10021 gcttgaccga tttggtaagt tacaccgtta tattccacat cagcagcagg gatacctgtt
    10081 ccaactaaag cttttccgtc cgtccattca atcaaatctt ttgcttttgc ttctgccaat
    10141 tttgttggat tagacaaagg catgatgatt ggacgtgggg tatgagccgc catttctttc
    10201 acgatttctt cagtgaatgc tccaggttgg gtagaagtac cgatcatgat cgttggatgg
    10261 attgctttta caactgcttc taagtttgtc aattcatcag cattagtgaa ttctgagcgt
    10321 ttacgagcga atgggatttg accaggagtc aatccttttg tatcttcaaa caataaaccc
    10381 tgtttatcta cttgatagaa atgttggcgt gcttcttctt caggtacacc ttgacggatc
    10441 atttcatcta aaaggatgtt tgcgatacct acaccagcag taccagcacc aaatgtcaag
    10501 atcgtttgat ctttcaattg ctcacctgat atattcaatc cacctaatac accagctagt
    10561 acaacgattc ctgtgccttg gatatcatca ttgaaagtag tgattttatc ttcgtatttt
    10621 tctaagatcg ttgcagcatt tcctcgtcca aaatcttccc aatgtaataa caattctggg
    10681 aataattcag tagctgattg aacgaattga tcaatgaatt cgtagtaagt ttcgccttct
    10741 acacgagcat gacgattccc taagtataat ggattattca ataattcttc gttattagta
    10801 ccagcgtcga ttgatacagg caagacttgt gcaggattga taccagccgc ggcagtgtag
    10861 accatcaatt taccgatagc gatgtcaaca ccgttcacac cccagtcacc catacctaag
    10921 attccttctg catctgtcac aacgatcaaa cgaatgtcgc gtccatctgc tgcgtttttc
    10981 aaacttgctt tgatattttc tggttcgtcg attgaaagaa atacagcatc ttgtggtttc
    11041 aagaaaattt cgttgtattg ttcgattgaa tccgcaacga caggatcata aacgacaggc
    11101 atgaattcaa ctaaatgttc gcccatcaat ttataaaaca atgtccggtt cgtattgaat
    11161 agattcatta agaagatacg tttttccaaa tcggtctgtt tgcttaagta ttgaccgtaa
    11221 gcttggacag attgttgttc caatgtttgg acagtacttg gaagcatacc agtgattccg
    11281 tattttgccc gttcttcttt tgtaaaagct gttcctttat ttagaaaagg attatttaat
    11341 aagtttagcc ctgtaagcat gaatgattcc tcctaagttt taatacaaac gaagtatagg
    11401 agtcagatga ggatatagtc aaaaagagca ggtataataa attttattta tatgagtaat
    11461 agagtcaaaa gaaatcaaaa tagcggtatt tgttcttttt atatcaacaa tccttgcttg
    11521 attactatgc gtatctctta tatgctaaag aaagaaaagt agaagaataa aggatatgtt
    11581 agatagataa atagaatcct gaataattca tagctttaat tctttggcac attttattga
    11641 aatatgtatc actaatatcc ctatcaactg attttgacta aaaagttact tcaaacattc
    11701 actgttcaaa aatattgagg aataaatttt gggtgtaagg cattttgagc atacttctcc
    11761 ctggataact tcccagtaaa aaaatcgtta gattgttgga ttagatccat tgactggctc
    11821 gcctcaggcg agcaaagacc ctgtagaatt cattctgata cacatgataa ctagataatt
    11881 tgagatagac tcgtctaccc gtttggacta atttcccagc aactttcaga aacttcaatc
    11941 gaatagaatg aatggtcatt cccttttgga cttcttcaaa gccaatcgtt cttaagaagt
    12001 tgactaagtt gtaagctatc aagctgagca tcattcggac atgattctcc aaaaagcgag
    12061 gactgtctgt tttgtcaaag taaaagccag ctttcgcttc tttaatgaag ttctccattg
    12121 tgccacgttt ggcatagata gaaaagatgg tatcaggaga aacattttct gatagattcg
    12181 tcacgataaa ttcatgtcga aagagtagtt cgcccgcttc acgtgttgag cgtatacaca
    12241 cgcgacgact ttgtgaccag gattgtgctt gataagtggt cgagaagtac tgaacttctc
    12301 gttcttccca cttttgatta tcgccataaa gtactgattt ctcagctatt tgacctagtc
    12361 tacgattatt cttcagtcga ataacataat gactcttttt tgattcacac gaatcataca
    12421 caccaggtgt agcgaacccg ctgtctccac gaaccaagat gtcagtgttt ggtagagagt
    12481 gattatagtg ctctaataaa ggtgtgagaa actcctttac gccttttgat gtatattgat
    12541 ttcctgaacg tagttcagct tttaagaagt caccagtcaa tccgtcaaaa gctaccaatg
    12601 gatggtaacc ataggtttgg tagtgtgtat tataattcgt ttgttcttgg tgaccaaatg
    12661 tatctgaatg ggtcgaatct aaatcaatga ttaattccgt atcattacgg atgaggcgtg
    12721 ctttatcgat aagttcttgg ttcaaagctt gaagttcatg gatattctcc tcagatagtc
    12781 gatctaaaaa tcgggaaagt gaagattgag aagcgagttc tttcctatct aaaacagctt
    12841 taaacacagg atcttgtctc aggagattag ctgcagaatc agctgagtaa ccagcaatta
    12901 attgcataat gaactgctca agtatggata agttgtcatg agtaaaatac gctcgttcgt
    12961 cttcaatgtg aatgtgttgc ttagccagtt cagaaaaacc aaagatgttc atcagctctt
    13021 tcactaggac gagacccgaa tcactcgata attgaccacc tgtatgagaa atagtgatat
    13081 ttgaattgaa ttttacttgg tttttgtgta agctagtcat gaaaagaact cctttctttt
    13141 ggttggttta gtcactttaa ccatagcaga aaggggttct tttttcatca cttaacgggt
    13201 gaagatgaaa aagtaattgt aagctgccgt gaggcagttt cacatggttt taattaaaag
    13261 tatgaattat tcaggtagaa tctctgaaga ataaatcacg tgatcgtgac gataaaatac
    13321 acttacgtca ggtgataaaa aatacataag tagaaaacta agaggaggaa cagaaaatga
    13381 aagaagttca attgatcaga aaaagcgaac tatcagaagg tggttgcaat gcatgcggcg
    13441 tagtagaagc aacaagctat acattaaagc ttggtgccaa caaagcgata atctctgagt
    13501 tgacagtagg cggtcttgtg gacagtctgg cgcttgctga aggttttatt ggagaagata
    13561 tttatgagat gttttcagaa atccgtcagt taaaaaaagg ggaaaactgc atagaggttc
    13621 atcatgagag cccgaatgtg agattcaaac gtggtgataa tgagatgatc ttcaataatc
    13681 atgtgtccga tcacaccgag ctatatggaa tcgtgaatca aatactaact gagctttttg
    13741 gattgggacc ttatgcgttc aaggaagaaa acggtaatcc aaaattaaac gaagaatggc
    13801 aagaaacgat tgaaatacaa agaaacaacc cgcacttatt tcaataggca tccacagtat
    13861 ttcgtaaaag ggactttgac acgacttttg tcaaaatccc atcgtttttt atctatttgt
    13921 gttctaaaca tttctttcag agtagtaaca actggaatga ctgaaatgta aacagattat
    13981 ttgcctgtat caatacggaa gagtaatata aagtgtgtaa acgctatcat attattcatt
    14041 caatttttta tagagggaag gcattgaaat ggaaaaaacg tatcaagcag gtacaaatga
    14101 aggaatagtg gattttatca atatggaaga tttagaaata gccgcttcac aagtgattcc
    14161 agctggtggt tatggttaca tcagcagtgg tgctggcgac ttatttactt atcaagaaaa
    14221 tgaacgggca ttcaatcatc agctgattat tcctcatgta ttaagagatg tagaattacc
    14281 cgatacaact acacattttg acaaagaaac gctgactgca ccaatcatca tggcaccagt
    14341 tgctgctcat ggcttagctc atgtgaaggc ggagaaagct tctgcaaaag gtgtagcaga
    14401 ttttgggacg atctatacag caagttctta cgcttcttgt acattagaag agataagaga
    14461 agcaggcggg gagaaagcac cgcaatggtt tcaattttat atgagcaaag atgatgggat
    14521 caatttggat attcttgaag tggccaagcg taatggagca aaagcaatcg tattgacagc
    14581 ggatgcaaca gttggcggaa accgtgaaac agaccgcaga aatgggttta ccttcccact
    14641 gccaatgccg atcgttcagg cttatcaatc aggagtaggc caaacgatgg atgctgtgta
    14701 caaatcttcc aaacagaagc ttagtccaaa agatgtagaa tttattgctg ctcattctga
    14761 tttgccagtc tatgtgaaag gtgtccagtc agaagaagat gtctatcgtt cattagaatc
    14821 tggtgctggc ggaatctggg tatctaatca tggtggccgt caactagatg gtggtccagc
    14881 agcgtttgat tcgttgcaat atgtggcaga agcagtagat aaacgtgtac caatcgtgtt
    14941 tgacagcggt gtacgacgcg gacagcatgt tttcaaagca atcgcatcag gtgcagatct
    15001 tgttgctatt ggcagacccg ttatttatgg attgtcttta ggaggaagta caggagtcca
    15061 tcaagtgttt gatttcttta aaacagaatt ggaaatggtc atgcaattag ctggaacaca
    15121 aacagtagaa gatatcaaaa aaataaaatt gcgtgagaat cgttttattt aagaaacaaa
    15181 tgacgaaaaa aataggagtg cggcagatga tgccgtactc ctatttttat agttcaagga
    15241 tgtcactaat agaataattt gaaacgaaaa aataaaattg aaatattttt tcatacttcg
    15301 ccgattgtaa aattaagcta gacaactaaa aaaagtcatt tgtgctacac tcaaaatagt
    15361 tccgacgaaa gaattataag gagtgagtac aaatgaccta taaccatctt acaccaactg
    15421 aactcgtaat gatagaagct tatttcaatc aatcacaacc tgtttcaaaa gtagccaatc
    15481 tgttacaacg ttctcgacaa actatttata aggtctatcg ttttcttaaa tctggtggaa
    15541 gcgcaatgag ctactataaa caatataaga aaaacaaacg taactgcgga agacatccga
    15601 ttgttttacc tgatgagcaa caggaataca ttcaaaaaaa ggttgttcaa gggtgggctc
    15661 ctgatgtcct tattggtcga gcagagtttc ctattgcttg ttcggtacgc acactgtatc
    15721 gaatgtttaa agataaaacc tttgacactc acgatttacc aatgaaggga aaaagaaaac
    15781 caaacggtca taaagaaaaa agaggaaaac aagggtttcg ccggtcaatc cgagacagaa
    15841 agacagacta tacccagttt gatcatgaat ttggtcacct ggaaggtgac actatcgtgg
    15901 gtggaaaaca taaaagcgcg gtcatcacat tagttgaacg gctatctaaa gtcatcatta
    15961 ccttgaaacc cgaaggcaga caggctaaag acattgaaaa taggctgaat caatggtttc
    16021 gttgtgtccc aaaaaactta tttaaatcca tcacatttga ttgtggaaaa gaattctcaa
    16081 attggaaaaa gattagtaat accaacgata tttcaattta ttttgcggat ccaggaacac
    16141 cttctcaacg aggattgaat gaacattcaa atggcttatt acgtaaagat ggtttgtata
    16201 aagctatgga ttttaatggt gtatctgaaa cctttatcca atcggtggca tcgaaacgaa
    16261 atcatattcc tagaaaatca ttacactatc ggacaccaat ggaagtattt ttgagttacg
    16321 tcacaaaaga tgatttgtct aacttaattt gacaaacgaa acttaataaa aaaggaagaa
    16381 tattcatgta aacgatttta ttgttttttt ctcttttata caaaaagggt cataatttca
    16441 agtaagcgga tgaattcatg aggatttttt gcatactgtc tctactatac ttaggtcact
    16501 aagaaaaagg agtgatttat tatgaaaaag aacatgattg ggacattatt actttcaaca
    16561 cttcttctag gcggcttggc aacaccagct tttgctgagg gtcaagcgac ttcaaaagga
    16621 gatattacct ttactgaacc aacaaatacc gtggagcctc ttaaccctac tgatccttca
    16681 aaaccagtag agccagcaga tccggaaaat ccagcaactg gccagacagg tcctttaaca
    16741 ctggatgtag ttcccgaatt gccatttggt acacacgaga ttgaaagcgg aacaaaaacg
    16801 tatcaagtag acgcttcaaa aaacgataca ccttatctcc aagtatcaga tcgtcgaggt
    16861 gtaggtgcag atggccaagc acaagggtgg aacgtgacag tttcggtttc agattttgta
    16921 aacggttcac aagttttgca aggcgctgaa ttagattttg gaacattcac tgttaaaagt
    16981 acgtcagata acgaatccac tggaccaact agtcaaactg taactgggtt gagtaaagca
    17041 agtgcagcga caccaatatt tacagcagca aaagatcaag gtctgggaac atggctttct
    17101 gtttatgatc cagcaaatat cacactgaaa gtgccgaaag cagcagctgg aacattcacg
    17161 gctgatttga cttggaacct cgtggctggc ccagtagcat agagtatgaa ataaattcaa
    17221 caaactgcgg tgaaaatact ataggtgaag gattttttac cgcagttgtt gttgaaagga
    17281 tgtggtggac aatgaatcaa agtaaacgac tgtttgtttc gtttttttct attttaagtc
    17341 tatttttcat ttttccgtca atcagccaag caaaggattc agcaggagac tttggaatca
    17401 aaccggtatt tccagagaac caaatagata aagcaatcgg ttattttgat ttattagtgg
    17461 caccggaaca aaatcagaca ctagaagtaa ttatcagcaa ttcttcagat gaagaacgaa
    17521 catttgaggt ctctgtcaat ccggcggtaa cgagtgatgg tggaacgatc gattatagtc
    17581 aaaaaaaccc aacgttagat gagacattgc cgtttgatgt ccgtgacgtt ttgctcattg
    17641 cgaaaaagga aatcaatgta tcagcacatg cagaaacaac tgtacctata gaagtgaaaa
    17701 tcccagcaaa aagctttaag ggacgtgtat tagcgggtat tcatgtttca cctaaagaag
    17761 aagctgaaac agaaaatgct acagaaggag cacaaatcaa aaatcgtatt gcttataatc
    17821 tcgcagtcgt tttacaagaa agtcaagaga cgatagaacc ggatctgaaa cttttatcag
    17881 gagatttgga cgaggtcaac gcaaagccaa ctgttcagct tcgtttccaa aatcctcagc
    17941 ctaggattat cagcaatttg atatttacga gcaagatctt ttatgaaaat cagctttata
    18001 ttgaaaatac gtctaatgca tttttagttg caccaaattc gaattttcac ttgaatcttg
    18061 atttagctgg tgataaagca aaagccggcg attaccgcgc agaaatcata gcaaaatcag
    18121 gcgacagtaa tgaatggcga tttacacaaa actttacgat caaaaaagaa aaagcacaaa
    18181 aagtaaatga aaattccgtt tttgcagtac aagaacaatc tttcccatgg ctttatgcag
    18241 gattggttgc tgcagttcta gtgttatggt tgattttgct gtttttgtgg gtccacaaga
    18301 aaaagaaaaa aggagaaaca acatgatgag gtgatttcgt tgaaaacatc gatcttattg
    18361 ggagggatta ttgcaagttt gctgttcttc agctggcaag agccagtcca agcagatacc
    18421 aaaagttcag gtacgattac attttatgat ctaagtccaa ttccgcatcc aagtggttca
    18481 gtcaatggcg catcaaaaca gcagacacag ctaccgattt ataaagaatc taatcaacat
    18541 ggcgtattcc cggagacgaa tgaacaaaag atccgttttc tttatttgat cattggtata
    18601 gaattatggc ttcttgcgtg gttgttgtta tggagaagaa aggaaaagga ggatgaacat
    18661 gttgctacaa aataaatttt cattttttcg ctccttcttt tttacttcgc tttttgtttt
    18721 aagtatattt atcttttcac cttcggcaga agctgttgga gcaacgcctc ctgccatttc
    18781 ttatgatcca tacacgtatg aaaatatcgt acctccaaat ggtggacgta gtgcagtcaa
    18841 tggcgggaag cttgtccaac taactggggc taaccaattg cctaaattga ctgctactta
    18901 tttgaacgat ggttcaaatg atccttggaa aggtggcacg gtccgttttc attgggaaag
    18961 ccatgtgaaa ctaacaaatg tacttgattt ggtgaatatc actgcacaag gatttgcatt
    19021 tgtggcgatc atcaatttgc cggaacatgt atcaagcagt gatgtattaa aagcaattga
    19081 ctggaacaac gcctacttgt atattgccgg tgcccccatc aaggttacta gcggaagttt
    19141 attatcagca ggaagccata ccttgcgttt tgggatgggg tcgtgggata caaatactct
    19201 attgacagat attattgcta ttattacgcg aggcgaattc aacgtggacg atatcccgct
    19261 tcaatttgat gttacagcta atgtcgcgaa gatgacagaa aacggagata aatatgatac
    19321 gagtccaaac aaaattttga ccaagggtaa attacaacct tctatgtcta aaaaggccgt
    19381 tttttctgtt gatttttatg attcgaataa tattatccaa gatcgtatgt tcaattcgat
    19441 tttagctcca gctgcaggta gattttatcc taaattaact ggcggaaact tgaatccagg
    19501 agcatatgca ttttctgcaa gtgcagagat aaatacgtgg aatagttaca tctcaccaag
    19561 tgattcaaca ggaaaatata atacatttgc tgataccaca gaatacgtgg atggaaataa
    19621 ccagaacctt ccaggctcta ccaatagtcg gacattgacg atggggctga ctgaaggacg
    19681 ttttgatcag ttgccattta gtcgctttga tcgcgtagtc aactatttta caggaaaaaa
    19741 cgttacacaa ggagcaatac tgactcatac tccagcaagc acaagtggat tgaatcagac
    19801 agccaatatc gtgtacagtg ggaaagatgc gcaaggcgga ttgctttctc ctgttgcttt
    19861 aaaggtattg gagcagtaca agctagacgg aacggtttta ccaacaaact taacagccga
    19921 tagtcagtgg ggaaagctcc tttcttctgc atcgtaccaa gtcacggcca attggtcagc
    19981 aactgaacta aaaaagggtt cgatccatta caggctttat tcgaaaggaa cccaaaaatt
    20041 agcgacagga tggacagatc aacttttcca aacgatccaa tcaaataacg ggggtgtaaa
    20101 tacagctact tctacactac ctgcattggc ttcagggcaa tattattttg attatcgctt
    20161 ggtggacgat gtacttacta acatgtatcc gtcttttgcg tacaaatggc agtctgaaca
    20221 agctggaatc accactcttc ctcaaataac agtggctaat tttccatctg tgagtgcaga
    20281 tagtcagcta aaaaacctta gcagacaacc tgagacagga gatcccctgt ctgctctatc
    20341 aggagatacg attcaagaaa atgtgacatt cacgttaaca aaatctgggg atgcgataac
    20401 cgacaaaaaa atcacgatct cacttcctga aaatactact tatatcaatg gttccttaaa
    20461 attaaatggt acaactatcg cagataccgg cattcaacaa ggagtttctg tcccagcaga
    20521 tctattgagc aaaataggag ataccattca tcttacttac aactatcaat taaatactgt
    20581 agatacttct ttacaatccg tcagcatact gacaaaggca gctgtacttt ccagcaatat
    20641 tacattagca gatggtgcta agcttcctaa cccagttgtc caaacatctg ctaaaacaat
    20701 tcttgtcccc aaacaagaat taacattagt taatgtacca gatgatttta catttggtaa
    20761 cgatctcccc aaaccgttga agacaagtta ttatgaatca aaaggcgatt tttcttttga
    20821 tgtgcgggat actcggcttc caagcacgtc tccttggcaa ctgacaggta cattgacttc
    20881 tttatttaag aatgatcaag gtcaagaatt aagcggaaca aaattatact tcaatcattc
    20941 tggatcaaag caattgattc agcaaggtca aaacacgttg atttatgaga gtgatggaac
    21001 agctaaagga gaggttctcg ttgactttcc cgataccgat ggtttactct tagaggtaaa
    21061 tagcagtacg aatgctcaac ctggcgcaac gtatcaaggc atggtcacat gggaactgac
    21121 agctgggcca accagctaag tgattagaat ttgtatggat ctgtgataac tgtcacagat
    21181 cctctttttc aaggatgaga aggaaacttc tgctgttaaa atcagaaatg cttgaaggag
    21241 tggacttgga acttttcagt ttgcttgaaa aaaatgaaca acggcaaatc aaacttttcc
    21301 aacttttatt agaggaaaaa ggagaggttt ccgttaataa cgtacttacc cgcttatcag
    21361 ttgatcgggc taccttgaaa gaagatctgt tttatttaca agctaacttg gaaaatatca
    21421 aacaattgga aattgttatc caagaaggga atattttatt aatcagacat ggaaatatag
    21481 cagcagtcga tgtttattat cattgttact tgcgctattc tacaaaatat cagatacttc
    21541 tttatctgtt ggaaaatggc tcatttgatc ggcagaaact tgtcatggaa cttaatatca
    21601 gttcggctac tttgtctcgc agagtgaagg aattaaatga ttctttaaat gagtttcatc
    21661 tgaaaataaa aagcgggcgg ctgaccggtc cagaaagtca aattcgttat ttttatttcc
    21721 aattattttg gtttggacgt ccctatcttg aaaacaaaaa agaatacagt gatttatcag
    21781 gaaacacttt tttagatctg ctgcaaaaag agtttagttt tgctattaca gaagaaggac
    21841 gattgaaatt tgaaatatgg ctttccatta tgaagaagcg aatgcgctgt acgaaaaaat
    21901 ccaaaccaga ttatagcaat cttcctgttg gattgtttca aacgacacaa tttttattgg
    21961 acctccatac atttttgacc cgttacttct ttcaatatgc ctttccttgg aatcaaaatg
    22021 aaacgttggt tttttatctt ttcatgatca gtaactttac attagataca cataactcgc
    22081 atgtccagga gtttttgata agcgaaaaat acaaagacga cgtagtagct caattgaatc
    22141 gaacgtttaa acaatcgata gaagaatatt tctcttttta tgtttttcca gaagagattt
    22201 cgcaaaagat tgcgttgaca gtgatgcaaa atcacgctca ttttgcctat tttagaggat
    22261 ggatgaatat ttttggccaa gaaggattaa aacagcggtt aactgaagta gaagataaac
    22321 aattcctggt tttatgtgaa agattggtca gacaaacatt tgctattgtt gattttactg
    22381 aagtagacga ggaaaatgcc cgctttgaat tatttgctcg ctatgcatct attttacgga
    22441 cgctgatcaa gcagttagaa ttacgtctgc aaattgctta tgatcttcct tatgaaaggc
    22501 taatggcgga tatcgtgaaa gaaatcatcc aggaacaatt gctgaatacg atcaaatatg
    22561 atttagtcga atacgagaaa gaaaagcagt atgatatcat tttgactagc caattgaaag
    22621 agtacccgtc acagaagtcc gctaaagttt ttgtattcac tagcaacgaa tataattatg
    22681 atttccccta tttgaaccaa tttttaaaag aatgttattt agaaaaattg aatttaagga
    22741 tgaataaaac atgaaaatcg tgatcattgg tgcttcccat gaaggcatca cagctgccct
    22801 aaatctaaga aaacttcaac cgaaagcaga agttttactg atcgatgaag atcataaaga
    22861 tggactaggc tatgtttcaa atggaatcaa cctttattta aaaggaaaga tacgtagttt
    22921 agctgaagtt gcccataaca tgcggactct tcaagaaagt ggggcaaaac tgatcaccga
    22981 atggagagta actgaacttg atccagacaa acatcagctc atcctcgcat ccaaagaagg
    23041 aaaaaacgag accataacct atgataaatt aatcgtagcg acaggttcct ctcctgtcac
    23101 cttgtataaa caaattgaag ctgaaaatgt ctacacctat aaaaatctag tgcaatctaa
    23161 gcaagtactt gccgctttaa aagaagcaaa agaagtagtg atcttcggtg ctggttacat
    23221 tggattggaa ttagcagatg ctttgagaaa taaaggatac attattcatc tagttgatta
    23281 tatgccgaat gttttatcaa gatatttcga taaggatatg atcaacagtt ttcaaaatca
    23341 gctgcaaact aagcaaataa atttttatcc aaatgaattc ttgatcgatt ggaaaaaatc
    23401 agaagagaaa gtagtatccg tacaacttct gtctcaaaca ataaaggcag atatggtcat
    23461 tttttctgcc caaactcgtc ctaatacgac attgttaaaa gaaaaagtgg ctttgtatga
    23521 ggatgagact gtgatagtca atgaatatct gcagacaagt gatccggaca tctatgcgat
    23581 tggagatatc gtcccagtca gttttgacaa aaataaacgg catctctttt tacctttagt
    23641 tactcgagct gtacatatgg caagagcagt agcgctaact ttgagtggtc agccaaccgc
    23701 ttacgacctc agacaaaaga taacagcagc agtcattata gactactttt taggaactgt
    23761 agggctgaca gaagatgaag cgccattctt ggaacaaaat accggttcgt gttcaggaga
    23821 atttgatcta tttccacaat acgacgaaga aaacaaaaca gtaaatgcaa agctgatcta
    23881 ccatccggat acattagaaa taattggcgg acagctgata agccaagaat tcttattatc
    23941 agatttgaac ctgcttgcgg atatcgtgaa acacaaaact acgattccac agctagcagt
    24001 tgaggatttt ggatttctgt ctgaatacac ctctcgtttt cactacctca atgagttagc
    24061 ttttaaagta ctagctaaga gagcaaatag caatagagta gataaaagac tgtgagaaat
    24121 atcacggtct tttattgtgt ggggatataa taaatattaa tcaatcgaga ataaaaaatt
    24181 catataatga aatagcggaa gaattttttg agcataatat tcgccaaaaa tttatcaagt
    24241 gataaaattg ttgtataaaa tgattatttc aggaggagga gttaaatgtt tttagcatta
    24301 aatgaaatta tgcattcaaa gttacgttat gcgttagttg caggggtgat gtttttgatc
    24361 gcctacctgg tatttttttt gactgggtta gcttatggct tagctcaaga taatcgaaca
    24421 gcagtcgaca agtgggaggc agattcgatt gtgctttcga aagatgcaaa ttcaaatcta
    24481 gggatgtcca tgattactaa gaagatagca gaagaagtcg aaggtgggaa agtggcgtat
    24541 cttgcccaaa cacctggagt cgtgactagt aaagacagta cagaagaagg aaaaatcaat
    24601 gttagcttct tcggtatcga taaaaatcaa ttcatcatgc ccaatcttgt tgaaggaaaa
    24661 gcttttgata acgatgatga ggcagtaggc gatattagtt tgaaagaaga atatggtcta
    24721 gccataggag atacagttaa gttatctggt agtgacaaga cgtttaaact aactggcttt
    24781 acggatcatg ccaaattcaa tgtctcacca gttctctata caaccatcaa tgcttaccaa
    24841 gaaatccgct tcgaaaaaga agatacaagt gaaaatgcac ggattaacgc aatagttgtc
    24901 agaggtaaaa tcagtaattt acctgaagat ttagaacaga tcaaaatttc taaattcatc
    24961 aatgaattac ctggatataa tgcgcaagta ttgacttttg gatttatgat cggatttttg
    25021 atcgttattg cggctattgt gatcgggatt ttcatttatg tactgacgat gcaaaagatt
    25081 aatatctttg gtgtaatgaa agcacaaggg atcacaggag gatttattgc gcgttctgta
    25141 gtggcacaaa cattcatttt atcatttgtt ggtattcttc ttggtctatt aggaacagtg
    25201 ggaacatccc ttgttctacc tgatgcggtt ccattccaaa gcaattggct gttttttggc
    25261 gtcattagct tgttgatgtt agtagtagct gtgttaggcg cactattttc tgttcggaca
    25321 atcgtaaaaa tcgatccgtt gaaggcaatt ggataagaag gaggaagaac atgaaagcaa
    25381 tagaatttaa agaagtagaa aaaagtttcc aagatggcga ccaagtagtt aaagcattga
    25441 aagaaacaac catgagtatc gataaagggg aatttgttgc aatcatcggt ccttctggtt
    25501 caggtaaaag tacttttttg actttggcag gcggattgca gacgcctagc agtggctctg
    25561 tatttatcaa tgatcaagaa ttcagtaatc aaaaagaaag cgaacgcgtt aaactacgtt
    25621 ttaaagaaat tggctttatt ttgcaagctt ccaatctggt tccttttctg acagtaaaaa
    25681 agcagttatt attagtagat aagattaaac atgaacatcg tcaaaaagaa gcaaacgaat
    25741 tatttgagca gcttggcgtg gataagttga tcaacaagta tccagaagaa ctatctggtg
    25801 gagaacggca aagggtcgca atcgctcgtg cattgtacaa tgatccgtcc attattctag
    25861 cagatgaacc aactgctagt ttggattcag aaaaggcacg agaagtagtc gatatcttgg
    25921 caaaagaatc aaaagacaaa aataaagcaa cgatcatggt tactcacgat acacggctga
    25981 ttgacaaatg cgatagaata tttatgatgg aagatggtgt attgaaagaa aaagaataac
    26041 caaaaagcag gtggaaaaat ggtccatcga tacaacaaag atacacagca aaaaattttg
    26101 aaagaagcga ctgacttgtt tatgtccaag ggatatctcg gcacttcaac aagagaaatc
    26161 gcacaaaaag ttgggatcac ccagcccaat ctctatcatt atttcggtga taaagaaaaa
    26221 ctatatacgg cagttttaga aaaccactta aaagatgttg gaaaagcttt gagagaaatc
    26281 gttcaaacaa gcgaaacggg atttcaagta accttgacaa aaatggcgca gtttttgatt
    26341 gagacacatc tggtcgattt gtttctgatg cttcacgatc ttgaaagcaa tctttcaaag
    26401 gaaacaagag atcatctttt ctttctatgg aagaaaaatt atcgtgaacc ttttgaagaa
    26461 atcttttcag caaaccaatc tgtgctaaga aatgggattt caaaagaaat cgcagcaagg
    26521 cattttttct tagtgttagc accttacatc actcggtcga aaagacctgt ggaagaaccg
    26581 ctaaatgtgg cgcaattgat cgatttatat ttgaatggtg tattaggaga agcgtaaagc
    26641 tgttatatag ggagaaaata gttaggtgaa gaagcaagat atgacgaaga tcgttatatc
    26701 ttgcttcttt atataggtag tttttcataa atacaaattt gttattctct catgattatc
    26761 agggtatatt tttatttatt atgtcggtgt tccaggaagt gttttaattt aataacgaag
    26821 aaattaattg tttgtagaat gaaggaaact atttattggg tagatgaatc accacgaata
    26881 gtcaaaagga gatttcagga tatgccaaaa atgaaaaaaa tcattttagc agtaatgtta
    26941 ttttctacgg ttatatcttt tacagcccct tttttgctta cttaagctga ttttaagttt
    27001 tggcccttag tctggatcta cgaggtgaga gtcaagaatg aaaaaaataa ttggagcttt
    27061 tgtatttgca atcattatca ttattatctg ttttcaaata gatgtttttg gtcaaaaaaa
    27121 gatagttgga caaacggtca gcagtacgaa cagtacggtt acaaagacaa aggaatcatt
    27181 tgatgataca gaacaagaga cattaatcaa aatcggaaaa aatgaccgta cactatatac
    27241 agatgactcg ctaactaccc cacttgcaac tataaacggc ggagagctgg cggagtttct
    27301 ttctgaaaca aaaaatgctt atcagatcaa gacaaatgat ggctatactg gatatttagc
    27361 cctcgaagat ggaaccaaag tcacaaagaa tatacaaaca aaaccggaag agctatctga
    27421 tgcagtgatt gtattagatc ctgggcatgg cggaaatgat actggggcat taagcaatga
    27481 tgaacaaaca gaagaaaaag atataacatt aagtacagcc ataaaagtaa aaaaggcgct
    27541 agaggatgct ggagcaacgg tttatttgac ccatactaca gatgaattag tacagcttgg
    27601 agatatatgc gactacagtg aagaaaaaaa ggcagatgtc tttatcagtt tacacgcaga
    27661 ctctacggaa tatgcgaatg aagcaactgg aatcacgaca tattattact atggacaaga
    27721 ggaaacatta gcacagacaa ttgctgactc cttcactgat ctgcctctgg attcaagagg
    27781 aatctccact gggaactatc aagttcttcg agaaaatctt caaccttcta ttttaatcga
    27841 gatgggttat atgaataatg atagtgattt aaaggaatta gtaacagaca gttatcagca
    27901 gcagattgct gcaagtttga cacaagcatt gaccacttat tttcagtaat aaataagccc
    27961 agccaaacaa aaatagctcc tcatattttt cgtttggccg ggcttattat cgcagatgca
    28021 agcttttaaa caccgtttaa ggaagaggtc gtgagcctgc cgttcaactc tgagtcacaa
    28081 ggagcaattt caaaaaacag cttctcatgt ttgatgacta ttgtgacttg tgaccgagga
    28141 gttggcatga gaacaccgtt taagaaaaag gttgtgaaat caacgttttg ttctgagtat
    28201 tagagaaaca aacggaaaaa tagttcctca tattttgccg tttgttgagc taataccgag
    28261 gatcagatga ttgaacaccg tttattcgga acaagggact gtgacatgac ttttgtcaca
    28321 gttccattat ttttttagta gcttcaaagc tttttctgta ttggcaaaat gaagtttggc
    28381 atatttttca agcataggca tgccgccttt ctccaatagt tttgccgcta cttggtcaga
    28441 tttcgttcca gctcctgacg gaagggtgat tccagcttca gctgatgctt tgtctaactc
    28501 ttccaaaaag ctggcacgac tgatgataga agcagcagca acagctaaat gatattgttc
    28561 tcctttagta acgaagtaca gtttttcttg taattgattt ttttctaaac gcgcatattt
    28621 tttaaaattt gcttccggcg taaactgatc gatcaaaatc gcttctggtt tttctggctc
    28681 gatttttttt agcagcaaat ggatcgcttg attgtgcaaa gcgactttca tccgaacggc
    28741 attgtatttt ggttggatct cattgtattt ttctggagat aagacaagca gtttataggg
    28801 gatggttttt tttaagatgt cagcaagctg gatgatttgg ctatctttta actcttttga
    28861 atctctaaca ccaagtttac gtaattctgc aagctgatca cgtttcacat aagcagcaca
    28921 tactgttagc ggaccgaaat agctgccatt tccgacctca tcactaccta aaacggataa
    28981 ttgactgata ttttttggca aagaagaggc tgtttttgct ggcttggctt gtttatcctg
    29041 tgcctccccc catttatcag cttctattgc tgcattcgta ccttgaaaca tcacttttcc
    29101 ggatgtataa gcggtaatgg tcgtcccgtc tttttttgca gcaaagctag tataaggaat
    29161 ctgtttattt aacaaataag agcgatagta tgctttcatt tgactaagtt ggtcgtttgt
    29221 tgctttgata atgacgttgc tcataaaatt ctcctcgttt ttatacataa aactataccg
    29281 caactcacga cttttcgttt tctaagagat tgcgagacat agcatttttt taaagtttat
    29341 catataatag ttaagtatgt gaaaaatatt tgtggagaac ggtgaaagaa ccaacttcat
    29401 ggtacactaa ataaaacttt catcaaatag gaggaaatga ttcgtatggc gcacgaaaaa
    29461 acacgctaca aagcggtgat tgccaaccaa acatatacta ttatcggacg ggaaaccaag
    29521 catcatatgg atatcgtaac gaagctgatc aatgaacagt tagcagaatt gaaacaatta
    29581 tcaccacaaa tggataatga acaagcagca atcttgatgg ctgtcaatgc gctttcggat
    29641 caattgaaga aacaagaaag aatattggag ctggaagaag aaacagcaga attaaagaaa
    29701 aaaatgatca aatttacaga gcttgaaaat cgtgtgaaac gcattgaagc aatcgagaac
    29761 gaagctcggg aagtattgaa agaaaatggt caagcagatc ataccattaa aaatcatgtg
    29821 gaagcacaac agattttaaa cgagaaacga aaaggacaaa ttaaacaaaa atcttctaat
    29881 taagatcctt ttatcaaaat aagaaaaatt taaaaaagtt agaagagaaa ggaacgtagc
    29941 atgctgagtt tgctgatttt atttttattg ctaattgcgt ttttttctgg agcacgtcgc
    30001 gggtttgctt tgcaaatcat attcgcgatt ggatatgtat tgtcatttat tgctgcccag
    30061 catttttaca aaccattagc aagtcatttg gaattataca ttccataccc agctgtcacc
    30121 ccaacatcaa agttagcatt ttttgatcag gtatttgcct ttcacctaga tgaagctttt
    30181 tatgcaggaa ctgcttttct gattattttg ctaattggct ggctgcttac ccgatttgtg
    30241 ggggtttttg tccatggatt gacgtatgtg ccgatcttga gacaggtaga ctggattgca
    30301 ggaggcatcc tgtcactaat catggcttac gttactatat ttttgatttt gcagttactt
    30361 gcctttgttc cattagatgt ggtccaaaat caattcggcg agcatagttt cgctcgtttt
    30421 atcgttgaga gaacgccatt tcttaccaac aagatgtatg agctttgggt aacgaatatt
    30481 ttgaattaag gaaaatagtg ctggaaaata tttcgttttt tgtacagagt agtttattta
    30541 agaaggctgc gtagcgtatg tccggcacag ctcttttttc atagtgaaag ataaggtgat
    30601 aaaatgaata accgaatcct tgaaacattg gaatttgaaa aagtcaaaca gatggtcaga
    30661 caatttgtag tgactgcaca aggaaaagaa gaactggcag aattggtacc agtgagtgag
    30721 aaacaaacga tcacaaactg gcttcaagaa actgaggatg gattgaaagt gcaacgtctg
    30781 cgaggcggga tccctatacc taaaattgaa aatatccgtc cccatatgaa acggattgag
    30841 atcggagcag acttgaatgg agtggagctt gcacaagttt ccagagtatt gtctacgaca
    30901 agcgaattga atcgtttcat tgatgatctt tctgacagtg aaatcgaatt tgcacgtttg
    30961 tatatgtggg cagatcagtt agtcacgatc cctgttttga gccgccgttt aaaggaagca
    31021 atcgatgaag acggacgcgt gactgatgac gcttctcctg agttaaagag cataagacag
    31081 aacattcggc gaagtgaaca agctgttcgt gaacagttag atgggatcgt ccgtgggaaa
    31141 aatgcaaagt atttaagtga tgcgatcatc accatgcgaa atgaccgcta cgttatccca
    31201 gtcaaacaag aataccgcgg tgtatttggc ggtgtagtcc acgatcaaag tgcttcaggt
    31261 cagactttat ttattgaacc aaaacaagtg gtggatttga ataatcgttt gcgtcaatac
    31321 caaatcgctg aacgaaatga gattcaacgt attttaagtg aactttctgc tgaacttgtt
    31381 ccccatcgtc aagaaatcat ccacaatact tatgtgattg gtaaaatgga tctgatgaat
    31441 gcaaaagcac gatttggtaa agaagtaaaa gccatcgtgc cgggaatcag tgaagacaat
    31501 catgtcgtct tgaaacaagc aagacatcca ctgatcgatc aggaaaaagt agtttctaat
    31561 gacatcacaa tcggaaaaga ctatcaagcc atcgtcatta caggacctaa cacaggtggg
    31621 aaaacgatca cgttgaagac tttaggtctg ttacaactga tggggcaagc aggattaccg
    31681 attcctgcag gtgaagaaag tcaaatcggt atttttgagg aagtatttgc ggatatagga
    31741 gacgaacagt cgattgagca aagtctatcg accttttctt ctcatatgac gaacactgta
    31801 gatattttat caaaagtcaa tgaaaaaagt ctggtattat ttgacgagct cggtgcagga
    31861 acagatccgc aagaaggtgc tgctctagca atcgcaatct tagatgatct tggtaaaaag
    31921 tctgcttatg tgatggcaac aacgcattat cctgaattga aagtttatgg atataatcgg
    31981 gcaaatacga ttaatgccag catggagttt gatgtggata cattaagtcc aacgtatcgt
    32041 ttgctgattg gcgttcctgg acgaagcaat gcgtttgaaa tctctagccg attaggtctt
    32101 gatacagaag ttattgatga agcgaagcag ttgatgaatg atgagagcca agatttaaac
    32161 gaaatgatca cagatttaga gaatcgccgg aaaatggcag agaccgaata tctggaaatg
    32221 cgtcattttg tttctgaggc acaagaactt cacgatgatt tgaaagaagc ttacagctat
    32281 ttctttgaag aacgtgaaaa agaaatggaa aaagcaaaga aaaaagcaaa tgaagtcgtg
    32341 tcagaggcgg aagaaaaagc agaaaaaatc attgctgata ttcgtaaaat gcaacagcag
    32401 attgggcaag gaaatgtgaa agagcatcag ttgatcgatg caaagaccca attagcaaat
    32461 ctacatcaag aagaaacttt gaagaaaaat aaagtattga agaaggcgaa agaacaaaaa
    32521 acgttgaaac cgggagacga agtgttggtt acgacttacg gccaaagagg gacgctttta
    32581 cgtaaaaacg ggaatcagtg gcaagtagaa atcggtatat taaaaatgaa tgtgtccgaa
    32641 gacgagctga cgcctgttgc tcctcaaaaa gagccgacac aacgagttat ccatgctgta
    32701 cgatctgaat ctagtagtca cgtgccgaat caattggacc ttcgaggaaa acgatacgaa
    32761 gaagcgctaa gtgaggtaga tcaatatttg gattctgcca ttctagctgg ttatcctcaa
    32821 gtgactattg tccacggaaa aggaacaggc gcacttcgca agggaattac tgattatctt
    32881 aaaaaccatc ggagcgtcaa aagctttgag tttgcaccgg caaaccaagg cggaaatggt
    32941 gcgacaattg ttaaatttaa gtaaagctta ctaaaggtaa gcagatggtt cgctatccaa
    33001 gtatagaaat gctatactag tcatagattt taaaaatgga ggtctttgat atgtcacaag
    33061 taattacaga taagacattt aatgaagaaa cagataaagg acttgtattg attgattttt
    33121 gggcaacttg gtgtggtcca tgtcgtatgc aagcaccgat tttggatcag ttggaacaag
    33181 aatatgatga agaagaattc agaattgcta aaatggatgt ggatgaaaat ccagaaactc
    33241 ctcaacaatt cggtatcatg agcattccaa cattaatgtt gaaaaaagat ggtcaagtag
    33301 tagaaaaagc tgttggtgtt cattcaaaag aacaactacg tcaaatgatc gatcaatatt
    33361 tataaaaact tacagcaaaa ataagctgag ggtgggactg aatccagttc cgtcctcggt
    33421 ttttttgcgg ttgagttaag gttattcaaa ttaatatttt ttgagtataa ttacgttgtc
    33481 aggctggatt gtttttttga cagacatgct ataataactt taattgaata tgaaggggag
    33541 taccagatga acgaacgaat caaaaacaaa ttagctttac ttcctgatca gcccggctgt
    33601 tacttgatga aagataaaaa tggaacgatc atttatgtcg gcaaagccaa aatattaaaa
    33661 aatcgtgtcc gctcatattt tactggtagc cataatacga aaacggaacg gcttgttagt
    33721 gaaatcgaag actttgaata catcgttaca gaatcgaata tcgaagcatt gctcttagag
    33781 atcaatctga taaagaaaaa tgatcctaaa tacaacatta tgttaaagga tgataaaacc
    33841 tatcctttcc ttaagataac gaatgaaaaa tatccccgtt tggtgattac tcgtaaggta
    33901 ctaaaagaca aagcactgta ttttggacca tatcccgatg tcggtgcggc gaatgagaca
    33961 aagaagatcc tcgatcgatt atttcctctg agaaaatgca aacctagtca gacgaaggag
    34021 ccttgtcttt actatcattt gggacaatgc ctgtgtcctt attatttcga cgtagatccg
    34081 aaagtctata cgaaaatcgt tgatgaagtg aaaaactttt taaacggagg acacgaaaaa
    34141 atcgagtcag agatccatca gaaaatggaa aaagctgctg aaaacatgga gtttgaaaaa
    34201 gctgcagaat accgagacca gatccgtgcc attgagacaa tcatgactcg ccaaaaaatg
    34261 acaaatactg atttactgga tcgagatgta tttggttatg cgattgataa aggatggatg
    34321 tgtgtccaag tgtttttcgt aagacaagga aagctaattg aacgagatgt ttcaatgttt
    34381 cctttctata acgaagcaga agaagatttc ttgacttacc tcggtcagtt ttatcaggaa
    34441 aatgagcatt tcattcctaa agaagtcttg attcctgata cgatcgacaa gcccagtgta
    34501 gaagctttgt tagctacgaa agtgctgcaa ccaaaacgag gagaaaagaa aaaattagtt
    34561 gaattggcga gtaagaatgc ctcagttgct ttgaatgaac ggtttgatct gattgccaga
    34621 aaacaggaac ggacgatcgg agcagttgaa cgtttaggaa acgcgatgaa tatcccgacc
    34681 ccaatacgta tcgagtcttt tgataactct aatatcatgg ggacgaatcc tgtggctgcg
    34741 atggtcgtat tcattgatgg aaaaccggca agaaaagaat atcggaaata taaaataaaa
    34801 acagtcaacg gaccggatga ttatgcatca atgagagaag tcatctatcg cagatattcg
    34861 cgagtgattc gcgaagggtt gcctttacct gatttgatct tgatcgatgg aggaaaagga
    34921 caagtagatg tggcaaaaga tgtcttagaa aatcaattag gaatcgatat tcctattgct
    34981 ggcatggtaa aaaacgataa acataaaaca aatgagttgc tattcggtac agatttggct
    35041 gtagtaccgc ttgagcgaaa ttcaccagag tttttcttac tccagcgaat ccaagatgaa
    35101 gtccatcgat tcgcaatcac tttccatcgg cagctacgca gtaaaaacag ttttgcttcc
    35161 cgattagatg aaattgaagg gttaggacca aaacgaaaaa aaatgctgct taaagagttc
    35221 aaatcgttga aaaatatcac agcagccacg acagaagaat tacaagaaat cggactgccg
    35281 aaaaatgtcg cacaaaacgt atttgataaa ttacatcagg aatgaggaaa gggcacttta
    35341 caaagaaagt aaagtgtcct atcattttat gtacaacaaa aagagcgttt acgttttgtt
    35401 ggtaaataat aaatcgctta cgtctggtgt ctaaggatat atgtgatttc ttgaagaagg
    35461 agtgaagaaa tgttagaagt caaggatctt gttaaaactt ttgggaacta cacagctgtc
    35521 gatcatgtct ctttccaaat ccccgatgga aaaatcatgg ggctaattgg ccaaaacggg
    35581 gctgggaaga caacgacttt tcgtttgatc ttggattttt tgacacaaga ccaaggagaa
    35641 gtgttatgga atggccatca tcttggggag aaagattatg atgtcatcgg ttatcttccg
    35701 gaggaacggg gactttatcc gaaagtaacg attcaagacc aactgcttta ttttgcagaa
    35761 cttagaggga aatcaagaaa agagattgaa ccaaaaatcg acttttggat ggaaaaattt
    35821 caggtaaaag ggaaaaagac ggataaagta aaatctttgt ctaaaggaaa tcagcaaaaa
    35881 gtccagctga tcgccacgtt aatccatgaa cctaaattga tcattttgga tgagccattc
    35941 agcgggttag atccggtaaa tgctgaattg ctaaaagatg gaatattgga attgaaaaaa
    36001 catggttcat gtgtcatttt ttctagccac aatatggata atgtagaaaa aatctgcgat
    36061 catttgatca tgttgcgaaa cggaaaaatg gttttgaatg gaaaagtaca tgagattcgt
    36121 gaatcatttg gccgtacgaa gttattcttg gaatctggtt taagccaaca ggaagtagaa
    36181 gaaatcgaag gcgtgaaaaa agtgatcctt catgaagaca atacgttaga gattacctta
    36241 gaagatcctg aagctgggaa agaaatcttt actcgtgcga ctcagttcgg ctacattcct
    36301 atgtttaacc agcaaccgcc aactttggaa gagattttca aattgaaagc aggtgaaatc
    36361 gatgcgtaag ttttggatta ttaccaaaga tgtctattta aaaaatgtta agtcgatttc
    36421 tttcttgatc atgatattag tcccttttat catgctgggt atcatttatg tagcaggaaa
    36481 ctttgctcaa caaaatagtg atgtcaataa aatcggcgta ttggctgata atgagcaaat
    36541 cagtcagcaa atgagccaag tcaaaacaga tgattttgag ttcaaagcag ttgattctga
    36601 agaaaaagca caaaaagagc tttcggaaga aaaaatcgat gcgtatttag tagtctcaac
    36661 tgagaatgat aagataaatg ggatgcttta tagcgaaaat tcattaggac aaagtacaca
    36721 actactgata caacaacaac tgactggctt gcaatcgatg cttcgagcga gtcaattagg
    36781 catttcacca gaagaagtag ctagcttgag ccaacaagca agtttgactc gacaaaaagt
    36841 caattttgat tcaaaaggaa aaatgacagt cggagaagat aactcgacag tccaatacgt
    36901 cgtaagttat gtagcaacga ttgtcttgtt cattattatt ttgacgtatg cgcagatcat
    36961 tgcacaagag atcgcttctg aaaaaggcac gcggatcatg gaagtgatcc tgtccagtac
    37021 gactgcacaa aagcattttt acggaaaatt aacaggtgtt ttacttgttg cattaacgca
    37081 aatggcatta tacggtatta tttttggtat aggatacagt caattcaaag atatggaaat
    37141 ggtcaaaaat atattagacg gtatctcatt ggataacttg attggaccat tcttgtggta
    37201 ttctcttcta ttcatgatct ttggcatttt gattttttct gttttagcag ctttatgtgg
    37261 ctctttggtc aacaaagcag aagatacagc aaaagcaatt ttgccagtga cttatctatc
    37321 tctaggcgga tacatgcttg gattgatact aggagcttca gatccaaaca atatcgttat
    37381 tcggatcact tcgtatattc cattcttatc ttcatatatt atgccgattc gcttagcaaa
    37441 tgagacagtc ggtatgggtg gagcattgat ttccttgttg gtacttgtag ccgtaacatt
    37501 cttattgatg atcttgtctg caaacatgta caaatcgaat gtactcgttt atagcgaagg
    37561 cggattatgg agttcattga aacaatcgat ttcaattatg aggaatgaac gaaaaaaagc
    37621 ataaaacaat cctaaaatac agaaggaagc tggacatcta ttgttcccag cttctttttg
    37681 tatagaccta ttttatttat tttactatat tggatatata ttcattgaaa atagtgtata
    37741 acaactttat aatattgagt ataccaattt ttggaggcgt tttaatggac cgttatttag
    37801 atttactttc agaaaaattc ccctcaacgg aggatgtcat cacggaaatc atcaatctcg
    37861 aagcaatttt gcagcttcca aaaggaacag agttattttt gagtgatata catggcgaat
    37921 ttcctgcttt tgatcatatt ctgcgtatcg gttcaggaaa tcttaaagaa aagatcaaag
    37981 accttttttc tgaacaaatg acggaaacgg atatcagcca attcactatt tttacggctt
    38041 atccagaata tgcattaacg actgattggt acaaagaaca agataaatcg cagctgatcc
    38101 atcatttgat cgatctttta cggtttacga cagtcaaata tacccggtca aaagttcgaa
    38161 aaggattgcc gaaagaatat agctatatca ttgaggaact cttgtatatc gatgaccgta
    38221 tcaatggaaa aaaagactat gtaaaaaaaa tcattgaaca acttgttttg atgaaggaag
    38281 aagaaaaatt tttaacgaaa cttgcacaga cgatccaaaa atctgtcatc gatcatttgc
    38341 atatcgttgg agatattttt gaccgaggaa ctcaagcagc caaagtgatg gatcgaatca
    38401 tggatttttc ttctgtagat atccaatggg gaaatcatga tattttgtgg attggtgcgt
    38461 attgcggctc tgaggcatgt ttactgacac ttttacggat tgcagcacgc tataattatt
    38521 tatttgaatt agagaaagaa tatgggttga atttacgtcc attgttttct tttgctcatc
    38581 aaacttatca gaagaatcca gtatttgcgc ctaaaaacag taaaaatcta tccgaaagag
    38641 agcaagaaga attagaaaaa atccatcagg cactttctat ccttcaattc aaatcagaac
    38701 atcagttatt ggatcgtcga cctgaattta aaatggacga tcgtaaattg ctggaaaaaa
    38761 tcgactatga agcatcaacg attgatttag aaggtcagct ctatgagttg aaaggaactt
    38821 gttttcaaac gatccagcca gatgatccaa aaaaattctt ggaagaggaa caaaaagtga
    38881 ttgatagttt gatgtattca ttccagcact ctttgagaat gcaaaaacat atgacgtttt
    38941 tgatcgaaaa agggggaatg tatctcacga acaatcagca tgtgctgttt catggctgta
    39001 tacctgtaga cgagaaggga cagctcttag catttgaatt agaccaatct tatcgcggaa
    39061 aagaactgct gggctttttt gaaaaacaga taactgaaag cgcaaaagat ctgactgcaa
    39121 aagaagattt agcaaccgat ttgatttggt atgcgtggag tgggccattt tcgcctttat
    39181 tcggcaagag taaaatggcg acttttgaac gttattttct cacagagcct catacgcacg
    39241 tcgagaaaag caatccttat tatcacttgc gagatgaaga gtggttcagt gaaaaaattt
    39301 tagcagaatt cgacacaaaa gaagaagggt cagcaatcat taatgggcac acgccagtaa
    39361 aagtaaaaaa aggggagtca ccgatcaaag caaatggaaa agtatttgtc attgacggag
    39421 gtttatccaa agcttatcaa aaaacgacag gaattgccgg ttattcacta ttatgcaatt
    39481 cttatggttt ccaaatcgtg acacattatc cgtttttgcc tattgaacaa caatttgcaa
    39541 aaaaatccga ccaaacaaat gtgaaaaaag taatcgaaca gcaactacca cgcaaactca
    39601 atcgtgatac aacaaaaggt atggaactgc agcagcagat tacccagctc aaaaggctgt
    39661 tggactatcg aaaggacgat tgagaagaat tccagtgaac attgttccat agactggaga
    39721 ctgagacaaa agtcgtgtcg cagtctcttt ttccggataa acggtgttct tctgccaact
    39781 cctcggtcac aagtcacact attcaacaaa catgaaaagc tgttttttga aattgctcct
    39841 tgtgactcag agttgaacgg caggctcacg acctcttggt ataaacggtg ttcaaaagcc
    39901 agtttctcgg tattaagcca aatttatgca aaaatgtgaa aagccatttt cacaaaattt
    39961 ttcttaatac tcgaaactaa acagcttttt cacaaccccc tcagaaagtc tattaaaatt
    40021 cattcacaaa gaaatcgaat aagcgctgtt cagatggatg tacacggtgg gtaagttccg
    40081 gatgccattg aacaccgagg attttatgca tatcatcttt gctttgaacc gcttcaacga
    40141 gtccatcgtt agaaaaagcg atggctttta ataaaggtga aagatctttg actgcctgat
    40201 gatggtaaga attcacaaaa tatggatcgg ggagaaggtt gcttaacaaa ctgtctgaaa
    40261 caatcttgac ttcgtgagtc gtaaattgcg gggcagtggg ctgttgttca tgttttactg
    40321 accactttgg atataatgaa agatcttgat aaagtgtacc ttccaaaacg acattcaata
    40381 gttggagtcc ccggcagact gcaaaaattg gttttctttg tttcaatgct tccaagatca
    40441 gtgcctgttc aaattggtct cgttgaatat ttgtttccgt tagctttgga tgaggatctt
    40501 ccatgtaaag ttgtggactg acatcttgac cacctgcaag aataagtttg tcgatttgtc
    40561 caatcagttg aggagctgaa tctggagaca tgactggcag aataagaggc aatccttgag
    40621 catctaaaac tgcatccacg aatccttgcg gtgtatagct cacttgattt ccttggaaag
    40681 tatctgttgc tcgaatcagt tgatttccgg caatgccgat gattggtttc atttgtattc
    40741 cccctaaagt agacttattg taacaaggat ttaaaaaaaa ttaaactatg taagacttgc
    40801 ccctatactt atctgttctt gctgtgttac tatctgtaaa gagaaatttc gtgtgaaatt
    40861 catgacgata gttgaaaata ttgcttaatt tgttataata aaaagtaagc agaaataaaa
    40921 aggttgggcg taagcttcaa ggatctagtt cttcaagggg tgagaaggac attttattga
    40981 aggggcctgc ctatactaag ttatctttag aggtacatac tttcaatgaa aaaagaaagc
    41041 atgtacctct tttttaaatc gaaaggaagt gaagcaagtg ttagaagctt tggaacaaat
    41101 ccgaaaagca gaagaagaaa atgaaaagcg tgctcaaaca ctgcggcagc agctgaagat
    41161 ctatgcaaca gaaaaagagc aggaattgga aacgatccaa acaaactatc gacaacaact
    41221 aaaacagttg gtggaagaaa aagaacaatt tgaaatacaa gaattagaaa aagaaaaaat
    41281 gaaattgaaa gcagaagcag acaaggtgca agcaactttg aaagatcaat atcaaaagca
    41341 tcagagtcaa gcgatcggtg cgatcattga aagggtgaaa gaaacttatg gccgtcacta
    41401 agatggaaaa agtgaccctc atttcagata aaagaaatca ggagaggatt ttacaagcag
    41461 tacaaggaat ccaaaacgtt gaaatccggg atctgtatca aaatagaaca aacaaccagt
    41521 gggtggagaa gtattttcca gataccacga ttattgataa agaagcaaaa ttaagcgctt
    41581 taggtaatcg gttgaaagac atccaagaag ctatccaatt catacaacac cacggcgata
    41641 gccgtcaaaa gaaactccat ttgaaacgtc aagaactttc cttacaagat ttggaagcca
    41701 attattccga agaaaatttc ataaataaat tgaaagaagt ccttgattta aaagaaaagt
    41761 gggaacaact aactgaagaa cgtgatcgtt tagaagaaga ggaagaatgg cttcttcatt
    41821 ggcaacagct ggatattgct ccaagtatgt atgcaagtaa gacgaccatt tttgagatgg
    41881 ggactgtgag tgcagcaaat tttgaaagtt tcaaggaaga actgagtaag cttgatgacg
    41941 tttatatgga agaaatagat tatacgccaa aacaagttta tctttcgtat gttgctttaa
    42001 aaaaatcagt tgctgttata gaagaaattg ctgggaaata cgggttcaat aaagaaacgt
    42061 atccttatga acaaaatcct aaagaaaaac tgcaagagac caagcagcaa ttatccattg
    42121 ttcacgagca gcaaaagaag ataacgacag cgttaggtca gtgcagtggc tatatccaag
    42181 aatttgaatg ggtagaagaa gtcacacttg ccgttgcaga acgagaaaaa ataaaagaac
    42241 ggtttatcca tgcgacttat ctgatcgttt tgcaaggttg ggtagatgca gaagaaaaac
    42301 agaatttgct gcatgtgtta aacgaaaccg tatcagaaga agatatatac gtcagtttcg
    42361 aaacaccaaa ctcagaagag attcagcagg aagtccctac taaattgaaa aaccatccgc
    42421 ttgtcgaacc ttttgaattg ctgactgaaa tgtacagtct gccaaaatat gaagaagtag
    42481 atccaacacc ttggatgaca ccattttatc ttgtcttctt cgggatgatg gtcgcagatg
    42541 ttggttatgg tttgctgatg ctcattggga caatactagc tcaaaaacta cttgttctac
    42601 cacgcgggat gaaacgtttc gtaaaattct ttgagattct ctcgatacca tcgatcatct
    42661 ggggattgat ctacagttca tttttcggac agtcattacc aaaagaactg tttggcatcc
    42721 gcttgccttt cccaatccta tctacgacgg atgacgtcaa tacgattttg attttatccg
    42781 tgattttcgg tctgatacag attttggttg gattatttgt ggcagcaaag gaaaatatca
    42841 ggagaaaaga ttacttgagt gctgtcagtg atggatttgc ctggcaaggg atcctaattg
    42901 ggatcgttat tgcgttggtt ggtgccatgt tgataaaaaa tacagcattt gtttaccttg
    42961 gcgctgggct atcgatcatt gctgccttat gtatcttgat cgttccgatt atccaaacgc
    43021 cgtctaaagt caaaggtgct gccaaaggtg cgtataatct ttacggactg accggctata
    43081 tcggtgattt agtcagttat acacgtttga tggcattggg tatttcaggc ggaagtattg
    43141 gagcggcatt caatatgcta gttgctttca tgccgccagc tgctcgattc agtgtaggga
    43201 tccttttgat catcgcttta cacgcactga atatgttttt gacactgcta agtgcctatg
    43261 tccatggcgc acgattgcag tatgtcgaat tttttgggaa gttttataca ggaggcggac
    43321 gtgcctttga tccgctgaaa acggcggaaa aatacgtcaa catcaatcac aagaaaaaaa
    43381 acaataaata attggaggat ttttatataa tgatggatta cttaattagt caaaatggtg
    43441 gaatggtatt tgcggtatta gcgatggcaa cagcgacgat tttttcaggg atcggttcag
    43501 ctaaaggtgt tggaatgaca ggtgaggcag cagcagcatt gacaacaagt caacccgaaa
    43561 aattcgggca ggctctgatt ctgcaattgc ttccaggcac gcaaggtttg tatggattcg
    43621 ttattgcctt tttgattttt atcaacttag gaaatgacat gtcagttgta caaggactga
    43681 actttctagg tgcttctctg ccgattgctt ttacaggctt gttttcaggt attgcccaag
    43741 gaaaagtcgc agcggcaggt attcaaattt tagcgaaaaa acctgaacat gctacaaaag
    43801 gaatcatttt tgctgcgatg gttgaaacat acgcgatttt aggtttcgtt atttccttcc
    43861 tattagtgtt gaatgcctaa gcataaagga ggaaaagcag atgaacgcca ttgaaaaaat
    43921 catctctcag atgaacgaag cagcagagca ggaaagagcg gcgttagaac aagaagagcg
    43981 aatgaagatc gatcaaaact ttgaacaaaa acgtacgcaa gttgaaacag aacatcaaaa
    44041 acaaaaagaa aaacaaatcg aattactcga aaaaaaatac cgtcagctaa ggaatcgcca
    44101 gcaagtagaa gtacgacaag aaaatttgaa tgcgaagcag gaatttttaa gacgattgtt
    44161 tgcagatgcg gtaactgaaa tggaaaattg ggatgaatct gaacaaatcc agtttatcaa
    44221 aaatgcattg tattcattac ctttgacagg aaaagtagca ttcattgctg gagaaaaatc
    44281 agcagcctat ctttctcaga cgttgttgga tgaatggaac aatgaacttc cctttatgat
    44341 ggttttaagt gatgaaactg ttgctgatca agcgggtttt ctgataaacg atcaaggagt
    44401 tcagtataat tttcttttca gcagccttgt acaagatatc caaggaacga tgagttttga
    44461 gattgccaat caattattcg aataaggagg gatcagtgta tggaatacca cgaactaaat
    44521 cccttgattc gcggaagaga gcttgaactt ttgtccaatg agacatttga acggatgatt
    44581 caaacaaatt ctgtcgaagc acttggtgaa atattgaaat ccacgattta cagtccatat
    44641 atttatgacg gatatgaata tgacttcgaa cagaatcttt taaaggagca gagccgtcta
    44701 tttcgctggt taaaggaatc agcaccggag cctgaaatcg tttggatcta tacgatgcgt
    44761 tatactttcc ataatctgaa ggttttgaca aaagctgaga tgacaaatca aaatctggac
    44821 catttgtata tcgacgatgg attttattca ttagaaacct tgaaggatgc cattcatacg
    44881 caagtttcaa atgaacttcc tgaaagcatt atggacagta tacgagaagt acgtgattat
    44941 tttgaagagt ctaccattct tcagggaatt gacgtgattt atgatcgaca gtttttgaca
    45001 gagcagcgac ggcttgccga taagctagga tatcaagaat tgctgacaga agtcattgct
    45061 tttattgact tgaccaatat cacaacgatg gctcgaggaa tattgcagaa aagaacatat
    45121 gggttcatga cgacggtact ttccagctcg ggaagtatcg aaaaagaagt atttttgaac
    45181 tttgtagata aagatatgga agattacacg aattttctat tagagacaga ttatgctgat
    45241 tttctgcgcc ctgtcatccg tgagggaaat attgatctcg taaagcttga acagttgaaa
    45301 gatgattatc taagttcatt gtatcaagaa gcacaaaccc aggctttcgg accactgcca
    45361 ctactagcct ttttgaacgc aaaagaggta gaaagcaaaa accttcgttt actggttatc
    45421 ggcaaaagaa gccatttcga atcagaaaca atcagaaagc gggtgagaca agtctatgac
    45481 gcataagatc ggtgtagttg gcgacaaaga ttctgttttg ccattcaaat tgtttggctt
    45541 tgatgttcgt tatggtacaa ccaagcaaac cgttcgtcag gcaattgaag aaatggcaaa
    45601 gaatgagtat ggcgtgattt atgtcacaga gcaatgtgca gcattgagcc cagaaacgat
    45661 cgaacgttac aaggaacaaa tgatacctgc tatcgtattg attcccaatc atcaaggatc
    45721 attagggata gggctggaag aaattcaaaa aaacgtagaa aaagcagtag gtcaaaatat
    45781 cttatagtat gaaaaggaga tagaaatttt gcaaattgga aaaatcataa aagtctccgg
    45841 acctcttgta atggctgaga acatgtctga tgccagtatc caagacatgt gtctagtggg
    45901 agatctagga gttatcggag aaattatcga gatgcgtggc gatgtggcgt ctattcaagt
    45961 atatgaagag acttctggaa ttggtccagg cgaaccggtt cgctctactg gtgaagcttt
    46021 atctgttgaa ttaggaccag ggatcatttc acaaatgttt gacggaatcc aacggccatt
    46081 agataccttt atggaaatca cacaaagtaa tttcttaggg cgcggagttc aattgcccgc
    46141 attggaccat gagaaaaaat ggtggttcga accaacagta gaagccggag aaacggtgag
    46201 tgctggagat gttatcggga tcgtagaaga aacaaaagtt ataaaacata aaatcatggt
    46261 gccaaatggg ataaaaggaa cgatcaaaac aatcgaaaaa ggttcattca ctattgatga
    46321 aacagtatgt gtcattgaaa ccgaaaatgg tgacaaaaat ttgacgatgc ttcaaaaatg
    46381 gccagtaaga cgtagtcggc caatcaaaga aaaattaaac ccggatgcac ccatgatcac
    46441 tggacagcga gtgattgata cattcttccc agtgacaaaa ggaggagcag cagctgtacc
    46501 tggtcctttt ggtgcaggga aaacagtcgt tcagcatcaa attgcaaaat gggccgatgt
    46561 ggacatggtt gtctatgtgg gatgtggcga gcgtggaaat gagatgacgg atgttttaaa
    46621 tgaattccct gaattgattg accctaatac aggagaatcg ttgatggaac gtacggtatt
    46681 gattgctaat acatcaaaca tgccagtagc agctcgagaa gcttctatct atacgggaat
    46741 cacaattgcg gaatatttcc gtgacatggg ttatgacgtt gctatcatgg cagactccac
    46801 ttcacgatgg gcagaagcac ttcgtgagat gagcggacgt ttggaagaaa tgcctggtga
    46861 tgaaggttat ccagcttatc ttggttcacg gcttgcggaa tactatgaac gatctggaag
    46921 agtcattgca ttaggatcag aaggtcgcga aggaagtata accgcaatca gtgctgtatc
    46981 cccgtctgga ggggatattt cggaaccagt cacacaaaat acattgcggg tcgtaaaagt
    47041 attctggggt cttgattcca gtttagccca aaaaagacat tttccttcga tcgattggat
    47101 acaaagctat tctctttatt caacagaagt agggaagtat atggatcata tccttcaagc
    47161 tgactgggca tccatggtca ccgagggcat gcgtattttg caggaagaac agcagctgaa
    47221 tgaaatcgtg cgtttggtag ggattgattc cctttcagat aatgaccgtc tgacattaga
    47281 agtggcaaaa tccattcgtg aagattattt gcagcaaaat gcttttgatg atgtcgatac
    47341 attcacttca agagagaaac aattcaaaat gttgaagatg atcttgacct ttggtgaaga
    47401 aggacgccgt gcgttgtcgt taggtgctta tttaagtgag atcatggacg gtaccgtatc
    47461 gattcgtgaa aggatcggac gaagcaaata tatcccggaa acagaattag aaaaactaga
    47521 tcaaattatt ggtgaaatca agcaaacgat gcaaacgatc gtttcagaag gaggcatgag
    47581 caatgattaa agaatatcgt acgatcggtg aagtcgtcgg tccattgatg gcggttgaaa
    47641 aagttgctgg tgtcaaatat gaagaattaa tcgaagtccg tatgcaaaat ggcgagattc
    47701 gtcgtggaca agtattggaa gtccaagaag ataaagcaat ggtccaaata ttcgaaggaa
    47761 caagtggtat caatttacgt gactcttctg tacgattttt aggccatcct ttagaactag
    47821 gtgtatcgga agacatgatc ggtcgagtat tcgatggttt gggacgaccg aaagataacg
    47881 gaccagagat cctgcctgaa aagcatttgg atattaacgg agaagtaatc aatccagtag
    47941 ctcgggatta tccagatgag tttatccaaa caggtatctc tgcaatcgat catttgaata
    48001 cattagttcg tgggcaaaaa ttaccagtat tttctggctc tggtcttcct cataaagaat
    48061 tagctgctca gatcgcgcga caagcaacgg tactcgattc atcagatgat ttcgccgtag
    48121 tctttgctgc tattggaatc acttttgaag aagctgaatt tttcatggaa gattttcgtc
    48181 aaacgggcgc aatcgaccgt tctgtcatgt ttatgaattt agcaaacgac cctgcaatcg
    48241 aacgtatcgc tactcctcgt atggctttga ctgctgcaga atatcttgct tatgaaaagg
    48301 gaatgcatgt tttggttatc atgacagaca tgaccaacta tgcagaggca ttgcgagaga
    48361 tttctgcagc acggcgagaa gttccaggaa gaagaggata tcctgggtat ctttacacga
    48421 acttagctac tttattcgaa cgagcaggaa gaatccgcgg actaaaagga tcggttactc
    48481 aaattccgat tctaacaatg ccagaagacg ataaaactca tccaatccct gacttgactg
    48541 gttatattac agaaggacag atcatcttga ctagagagct ttacaagagt gggattcagc
    48601 caccaattga tgtactcccg tctctttcgc ggttaaaaga taagggaact ggacccggaa
    48661 agacaagaga agaccatgca gctacgatga atcagctttt tgcagcctat gcacaaggaa
    48721 aacaggcaaa agaattagcc gtagttttag gtgaatctgc tttatctgat gtagacaaga
    48781 tctatgcgaa gtttgctgag cggtttgaaa aagaatatgt caatcaagga ttctacacga
    48841 accgttcgat tactgaaaca cttgatctag ggtgggaact gctatcgatg ttgccaagaa
    48901 ccgaattaaa acgaatcaaa gacgacatgc tggacaaata tctgccagaa gggaagtgag
    48961 ttctgatggc acgattgaat gtgaatccga cccgcatgga attgactcga ttgaaaaaac
    49021 agttgacaac tgccacaaga gggcataaat tattaaaaga taagcaagat gaactgatgc
    49081 gtcaattcat cctgttgatt cgaaagaaca accagctgcg tcaagaaatg gaagagtcta
    49141 tgacagaagc aatgtctgat tttgtattgg caaatgcttc gatcacggaa cctttcatcg
    49201 aagaattatt tgttttgccg gcggaaaacg ttgatttgtc tatcgtagac aaaaatatca
    49261 tgagtgtaaa agtacctgtg atgaattttg aatatgatga acagctgaca gacacacctt
    49321 tggaatacgg gtatttgaat tccagtgccg aactggatcg gtcaatcgat cggtttacta
    49381 ccctgcttcc aaaacttttg gaactgacag aaatcgaaaa aacttgtcaa ctgatggcga
    49441 gtgaaatcga aaagacacgt cggcgagtca atgcattaga atatatgacg atccctcaat
    49501 tagaagaaac gatctattat attcgtatga agctggaaga aaacgagcgc gcagaagtaa
    49561 caaggttgat caaagtaaaa aatatgggta ccactgacta agtggtatct ttttttaaaa
    49621 gaggaaaacg atgattaaac aaagcaaaca aataaagaaa cgtttgccgg ctgttcagct
    49681 tctcgcatta ggattttttc tcctgatttt agttggcggc ggcctactta ccctaccatt
    49741 ttttagtaat agtgggcatg ggacaaattt tatcgatgcg cttttcactg caacttctgc
    49801 tgtctgtgta acgggattga caacattgaa tacagcagag cattggaaca gtgctggtca
    49861 atttttgatc atgcttttga ttgagatcgg cggtcttggt tttatgatgg ttccgattct
    49921 atgctttgca ttgataaaga agaaagtcag tctcagcaca cgtattatat taaaagaagc
    49981 gctgaatcta gaagaagtat caggtgtgat gaagctcatg ctttacatcc taaaattggc
    50041 ctttgtgatc caattgattg gtacggttgc tttatctttt gtatttgtgc ctgaatttgg
    50101 ctggttgaaa ggtatctggt acagtttgtt ccatgcaatc tctagttttt gtaatgcagg
    50161 gtttgatttg ttaggagaca gtttagccaa tcaccagcaa aatgtttatt tgatcatggt
    50221 ggtatctgct ttgatcattt ctggtgggtt aggcttcatc gtatggcgtg atttactgga
    50281 atatcatcag aaaaagaaga tgacgattca ttcaaaaatc gcattaaccg ttacaggtac
    50341 ccttttatta ggcggattcg ttgtgttctt tttcacagag cgcaatgggg caacgctagt
    50401 ggaagggaat atgtttcaac gattggcaaa cacctttttt atgagtgtga cacctagaac
    50461 ggctggatat tattcagttg attacctcca tatgagccat gcaggtctga ttttaacgat
    50521 gtttttgatg tacatcggcg gaacatctgg gtcgacggcc ggcggattga aaacaactac
    50581 gttaggtgtg ttgctgatcc agatgcgttc gatgttgaaa ggacgtacga gagcggaagc
    50641 atttggccgt accatccgac aaggcgcagt gttgcgggca cttactttat tttttgttac
    50701 gttgacgttg tgtgtagtcg ccatcatgat tttatcggtg actgaaacga tacctaaaac
    50761 ttctggcatc gaatatattg cttttgaggt cttctctgcg tttggtacag taggtcttac
    50821 gatgggattg acgccagact tgactgcaat agggaaattg atcattatag cgcttatgta
    50881 cattggacga gttggtgtat tgactgtggt cttctctctg atgacaaaag cacaaagaac
    50941 acaatcaaaa tacaaatatc cagaagaaag tgtcatgatt ggataatttc atcttctgac
    51001 agaaacctag tgatttagta aaataagaaa ataaaataat ttttcttgct tcttggatac
    51061 tttaagtgca aaatgttcgt ttattttttg aggcactacc cgttgttgaa cctcgggtag
    51121 tgcctctttt ttattgaaag gaatcaataa gatttgattc ctttatttat caataggtta
    51181 taattcatat ttttttattt tttcttttac gatacttagt ccagaagcta aaatttcaag
    51241 gttattagag gatagggaaa ttcgaacgaa tgcatctggt tgcgcacgtg tagtaaagcg
    51301 tctggaatga taaagtcgga taccgttagc aagaaagtca ttctcaatta tcttagggtc
    51361 aatctcagga tcaactggaa gaattcgata gtaagggaca cgattcgttg gtttttctac
    51421 tttgaaaaga gaatcaaaaa gctgattggc tctttttgta agacttaatt tagtttctaa
    51481 gatcctattt gcttcatttg aatggaggat ttgagtgatg acttccacat ctaatccaga
    51541 agttttcaca tttatattaa aaattgcttg ttctatcttg ctttggacat gtactggaac
    51601 tacaagataa gcaactcgca atccagaaca aataaatttt gtcattccag ctaagtagat
    51661 tgtttggtta ggtaataaat tttggaaagg aggaatgatc gtatgttgat agtaagttgt
    51721 taaaaatgaa tggatatcat cttcaataac ccatagacct tctttttcaa tcacttcggc
    51781 taattgctct cgtcttttgt gagagatttg aaaccctata ggattattac aagaaggcat
    51841 aagaaagaca ccatgaatct tcttttttct acattcctga atcaatatat caggtcgcat
    51901 tccttgctcg tcaaaatcaa ttggaacgat ctctaaatga tataactgag ccaattcaat
    51961 gaaattagcg tatgtatatc ggtctgttgc tattctatca cctggagaga aaagtgcaga
    52021 aagtgtgata gccaatccat tttgaacacc ggaaacaata gatagagatt ctaaggacgg
    52081 aatgactcct tgagttttta accaatttgc tgcagtagtc aattgaaaat ttgtgccttt
    52141 gggatcttgg taattaagca aaccaacaag atttgggtta ttactagttg attgaataaa
    52201 cgggacaacg aatttattac attcttcaaa agaactaacc agaccaaaat caatgatatg
    52261 ttcggttact tcattggtag agatggtgat ggactcaaag acattgggag aaataaatgt
    52321 gccacttcca atattcgtat acagcaaacc tttctcaatt ccatatttat aagccttgcc
    52381 aacggtagta aaatttaaat ctaaataatc agctagctct ctttgggagg gtaactgagt
    52441 gtttttctgt aatttattct ttaaaatatc ttgttctaat tgttgaatca acgatcgata
    52501 ataaggacgc ttcaattttt gtttgtctgg tcgccaagtt aacgggtaat tgtcataaga
    52561 attaataggc attttctcca ctccttaaaa ttgtatacca tacaatttta ccattgattg
    52621 tattgatttt agaagttaat cttttgtgag gagggaattg tctatgaaaa ttattaattt
    52681 tgaggaagta gtagcttctt ttagttttga agaagcgatt caagtaatga aaaaatgttt
    52741 ttttgattat gaaagtggta aaatcagcca aaatcctcga ttggtggaaa ttttacctga
    52801 cggaaaaggc aaaaatatgt ttgcgatgat gcctgcttat ttgggggaga acagatattt
    52861 tggcacaaaa gtcattaccg cttttccgga taatcatcag tatcatttac cttctcattt
    52921 aggagagatt ttcttgtttg atgctcaaaa cggactaccc aaagcattga taaacgcaaa
    52981 tgcagtcacc tggattcgaa cagctgctgt atctgcgctg gcaacagatt acttagcaaa
    53041 tccgcatgca gaaacacttg ctttaattgg agctggtcaa caagcattgt cgcatttgac
    53101 tgcgatttct accattcgtc ccatcactac agtgtttgtt tacgatattt tagaagaaag
    53161 acgaaatact tttatcgcta aagctaaaaa acagtatcct aaaataacgt ttataaatgc
    53221 aatatcagta gaagatgcag tacgtccagc ggaaatcatt tgtacgttga cctctagtaa
    53281 agaggcattt cttcaaaaag agtgggtaag ggaagacgca catatcaatg ctgtgggcac
    53341 atttactcct gatacaagag agataagtac atcgttgatg gaaacttctc aaatctatgt
    53401 agatgattac tcagctgcct taacagaaag cggggacctg cttatagcca tggcagaagg
    53461 agatttagag gaatcttgta ttgttggtac attaggcgaa ttagtttctg agaaaaaaac
    53521 attttataaa ggagaatcga ggccgacgat tttcgacgca gttggtctag ctattgaaga
    53581 tctttgttgt gcagaatata tttataacaa attagagaaa ggggctttaa aatgaactta
    53641 gattattacg tagttgatgc atttgcagat gaggtgttca aaggaaatcc agcagcggtt
    53701 tatgtgctag aagaatggct gccagaaggt acgatgcaaa aaattgccat tgaaaacaat
    53761 ttatctgaaa cagcatttac agtgaagaaa aaccaagaat ttgagcttag atggtttacg
    53821 ccagatcgag aaatcgatct ttgtggtcac gcgacattag caacagcatt tgttttattt
    53881 aactattaca aaatacctga tgaaacgatt aaattttcta ctcaaagcgg taatctattt
    53941 gtgacaagaa ttaaagatta ttattacatg gattttccta gtattatgcc gaaaaaagtg
    54001 ccgatattgt ctgaatatga agaagcgatt ggggctaaga ttaaagaagc ctacttagca
    54061 cgtgatttat ttttcgtatt agaagatgaa aaaacggtgg ctaaattgca gccagatttt
    54121 acagctatca aaaattttga attaggaata ggagtcatcg tgacagcaga aagtgtgcaa
    54181 aaagactttg tttctagaac cttctttcca aaattaacaa tcaatgaaga tccagtatgt
    54241 ggatcagcac attctaatct tattccttat tgggcagaaa gaatgaataa aacaaaattg
    54301 tcagcttatc aaatgtcatc aaggggcggg gatctagaat gtgaattaaa agaagatcgt
    54361 gtgataatag gaggaaaagc agttttgttt tctaaaggta cctcgtttat ttgatttctt
    54421 tcaaatgaat taaaaataaa ttggtggaga gtggcaacct tatagctaca atggttgtta
    54481 aaaaaagacg caaacttttt caatttaatg aaaagtttgc gtctcatttt aattattggt
    54541 gtttgttttt tattcaatca agagtaaaat gccccatact aactatcctc ggatgacttc
    54601 gattttataa ccatcagggt caatgacaaa gtagtaagaa ggcgcagtgc caggtaatcc
    54661 ttttaagtca gtaatctcaa gaccagcagc tttatgttgc tcgtgtaatt tttctagatc
    54721 atcagctgct atagcgatat gaccatagcc gtctcctaga tcatacgcac catgatcata
    54781 gttgtaagtc aactctaatt catagtcatc cccaggaagt gtcaaataaa caagtgtaaa
    54841 tttgttttct ggaaaatcac ggcggcggct ttcttcaaaa ccaaatgcct tttgataaaa
    54901 ttcgattgat gcttcaagat ctttcactcg aacacatgtg tgagccattt tcatagattc
    54961 atctctcctt ttccttttac tcaatcataa caaaaagaag taaggaaata gttgatttgt
    55021 gcttgttcat gagaaatttc tcatgtaaaa tggatagaaa ataaaactgt taaggtggaa
    55081 actattcatg gataaaccaa tttttggtga aaaaaagcaa tttgctgatt atcaaacccg
    55141 ttatgcagca tatattgtca tagaaagaga tcagcagatt gcagtaatcg aggctccaaa
    55201 tggtgctttt tttcttcctg gcggggaaat tgaaggagaa gagacgaaag aagaagcgat
    55261 tgtccgtgag ctgatagaag aaatgggaat tgtggcagaa atttcttctt atctgggaga
    55321 agctgatgag tatttttact cgaattatcg tgcaacttat tattataatc caggatattt
    55381 ctttgttgct gattcttgga agcgtatagg agaaccaact gaaaagacca ataaaatttg
    55441 gtgggtgagc cctcaagaag cgcttgtgaa attgaaacgt ggtagccacc gatgggcagt
    55501 cgagagatgg atcgaaaaaa ataaaaactg aaagaaagcg agtagatcag ctttctctca
    55561 gtttttttat ttattacagt acatattttt tgattgcttc agctacaccg tgctcgtcgt
    55621 ttgatgaagt gatgacattt gctgcttctt tgacaagaga aacggcattt cccattgcta
    55681 ctcctagacc agcatattca atcatggata agtcgttctc gttatctccg atagccatga
    55741 tttcatcttg atcaatacca aggtgctgtg cgagattcgc aactgccgca cctttacttg
    55801 cttctttatt tagtacttcg aagtaatagg gcgcactttt gaccgttgtg tatttatcac
    55861 ggaaagtttg cggtaaacgc gcaattgctg catctaaaat ttctggttcg tcgatcatca
    55921 tcattttgac gatgttcatg tctggtgtca tttcttcagg tgttcgatat tttagcggca
    55981 tcttgactaa acttgcttcg tggatcgtat aagcgctgat atctcgatta ggcgtgtaga
    56041 tcgctttgtc atcgatcgaa tggagatgac tgccgacacg gcgtgccatt acttcgatct
    56101 ctaggaaatc ttcatgtgtc agtccgtgac gggcgatgat tttacctgat ttcgtttgtt
    56161 gtacgagtgc cccgttataa gtaataacgt aatcattatc ttgaaaaagg ttcaactcgt
    56221 ttaactgttc ctggacacct ggtaagggac ggccagtgca taaaacgatc gaaacacctt
    56281 ttgcaacagc agcggcaatc gcttctttta cttcttttgt tacttctctt ttctcattta
    56341 ataaagtacc atcgatgtcg atagcgacta gtttgataga cataatttcc tccaatatat
    56401 tttaatttat ttcaatcaat gcgccattac ggatatggct ggcaaattct tgataagttt
    56461 cgtcaaataa atcataatga tcacgcaagg acggatcaac catttctttt ggaaagaaaa
    56521 atcgctcatc tccttgttct tgtcccgcta atgcagcaac aagctgacta acctgtgata
    56581 attctatcaa agttccgtca tttcgcaaaa tctcgatttg tgtgcgatgt ctgccttgtt
    56641 ctgggcgata aaaatcataa ggtagatcgt agctagaatt cacagcagtg taataaacag
    56701 gattatatcc gaccttttct actaacaaag tgagttcttg gactaatttt gaatcccggt
    56761 tgccagaaaa agtagcagat ttcagtggtt tacgattcaa aaaacgttta gctaaatcat
    56821 ttaaaatagg atcagcagat tcagaccatt gagtaaagta agtgcccaag accccatcat
    56881 caagtttgag gtattcttct aaagtaaagc ttccgtcaag aaatggcaga agtaattgag
    56941 aatggagttc aaaactgctg gcatcttctt gatacaactc gtgtgcgcga tgaagcagat
    57001 gctccaaaat tacttccata ccacgggaaa caggatggaa gtacacttgc acatacattt
    57061 gatatcggct aacgatataa tcttccactg catgcatgcc actcatggaa aaagcgatcc
    57121 cgccttcata aggacgaatc acacgtaata ttcgggtaag atcaaatgtt ccatattctg
    57181 ttccagtgaa ataagcatcc cgaagcaaat agtccattcg atctgcatcg atctgactag
    57241 aaatcatttg gacaacttgt gggttggggt aagttttttg gatcacacta gctacctttt
    57301 caggaaaacc ttcttctact cggttaagaa tctgatacac ttctgtttct ggagaagtga
    57361 tgatctctac agtgatggct tcatgattcg tatggaagat atgttcaaat gtatgagagt
    57421 aaggaccatg accgacatca tgcaacagtg ccgcacataa agtcaccagc cgttcctgat
    57481 catcccagcc tccgttgcct attttttctt ttgaaaaatt tcgagaaaag atatcgcaga
    57541 ttcgtcttga aatctcataa acgcctaatg aatgagtaaa acggctatgt tctgctccgt
    57601 gaaaagtgaa agaagaagtg cctaattgtt taatgcgtcg tagacgttgg acttctttcg
    57661 aattgatcaa atctaagatt acttgatgct ggacatggat atagttatgg actggatcac
    57721 gaaaaacttt ttccataggc agcatttgat atttataagg ggttgtcata atgtagctcc
    57781 tttatgttat tacgttgttc cattctctgg atttggttgt tccattgctc cagctggatt
    57841 tcttttttta gccagtcggt agaatcctgt aaatcacttg cagaatacag cttttcaaaa
    57901 gcttttgcaa atgcttcttt gacttctttt acaaaaaggg gttggtcaag cagttcttca
    57961 agtgttgcca ttgtagcagg atccactgca ggatagccat ttgtaccaaa gtcttctttt
    58021 aaagcagatt gataaaatcg cttcatgact tgcccacgtt tttcctgatt accgttgaca
    58081 cttaagtaca tcataacaga aatcccattt ttgatccgac gctgagcgat accggcaaat
    58141 tttttgccat ggatgcttaa atcataagtt ccaggacaat aagaatgggt gatttcgaat
    58201 gcttgtatcg gtgcctttgg aaaagcgtat ttagtcaata ggagcatttg ctcgtaagcg
    58261 tcatctatgg ttattttttt tgtgtcactt tgggggaaaa tcaaagaaat attcaaaatt
    58321 ccttcatctg atacgatccc taatccacct gaatttctga tcactggagc gtaacctgca
    58381 tcttccagtg tttggatccc gtcacttaga aaaggagttc gaacatcctt catacctaag
    58441 atgactgtgc agtcatattg ccagaaatgt aaaaagattt gctggtgttc tttagaatat
    58501 tccgttaata tatctgttaa tgcaaaaggg gaaaagtcgt ttttttgata aatgtgctga
    58561 tctagtcgaa tcgcagggtt cattcaaccg gctcctttgt ataaattcat attttctaca
    58621 atattatact ctaaaatcaa agagttgttt tggaaaaaag gttttctgta aaaatggaca
    58681 agttggttga caaaacaaaa gaaactagga acaatgtagc tatgagaagt aaaggagaat
    58741 gaataatgga attatcacaa ggaacaccga tttcaatcaa attgcgtaca aaagtaaaac
    58801 agcaagggga agtgcaagat ttttattttg atttgaaagg gcaaatggtg acaattggcg
    58861 atacgctgta catacgttat aaagagattc aggaggagac gggcgatgag attcctgtaa
    58921 cgatcaaatt agttccagat ggtcatgtac agctgatccg tgcaggagaa atgcggatgc
    58981 gtttgaaatt tggctataaa gaacggttag aaacatccta tcgaacacca tacggtatga
    59041 tccagattgc tacttttacg aaagaactgc atgttagttt gaaagaccgt cccacagcag
    59101 ggaaggttcg aatcgattat gatctgttca tgggaccgga aaaaatcggc gaatattatt
    59161 tgaccttgga ttttacagcg taaatataaa agaaaagttt gattttttgt gtctgatttt
    59221 gtatgataag gagtagactg tgaaaggacg tgtcctgatt ggaaattagt gtatttgaag
    59281 gtgcaaacaa aagcgaatta tcaatgatcg aagtagcaca cgcgatctta gaacaacgtg
    59341 gcgatgtcat ggatttttct gatttagcta accagatcca aaactatctt gggaaatcag
    59401 acagtgaaat tcgtgattca ctcgcacaat tttatactga cttgaatatt gacggcagct
    59461 ttatctcatt aggggataat cgttggggct tgcgttcatg gtatgctatc gattcgatcg
    59521 atgaagaagt aaaccatgga atggacgaag aggatgaaga tactccacgt cgccgtaaac
    59581 gcaagaaagt caacgccttt atcaatgatg atgaagacgc gatcgattac aatgacgatg
    59641 acccagaaga tacagatctg acagaagaag atgacgatga tctgtttgat gacgatgatg
    59701 atgaagatga agaaatcgct gcttataatt ctgacttgca agaaatcgga gcagatgacc
    59761 cgacagatga tgaagaagac ttgccaggaa atatcgaaga agatttaagt atcatagacg
    59821 aagacgatga tgatgaagat tatgaagagg aagaattttc tgactctgaa aaagaataaa
    59881 cattattttc ttacgagagg ttgtgacaga gacgatcagc ctatcagaat aaggaggtgt
    59941 gacacgattt ttgtcacacc ttcttttttg tatataaaaa tcccaagtaa atacataaaa
    60001 aataaaaaaa ttgaaacttt tagcgcacct tgtacgtcat attaagtgag gcagaaagga
    60061 gtggagaatg atttttactg gatataagaa aaaacggcag atcgaaaaat accgtcacct
    60121 gctgaatgga ctcatcgaaa ctgattatca aaaaatgttc cgtatcacga tgaattacgt
    60181 acatgataaa gaagaggcct tagatgtgat gcaagatagt tttcataaag ccctgaacta
    60241 ttttgaaaaa gaaaaagaga tcgagcattt tagtgcatgg ttttatcgga tattgatccg
    60301 cacggcatta gatagttggc gcagacaaaa aaggacgcct gtggacttat ttgaaattca
    60361 agatatcccc caacagttac ctttagaaac ttccgttgca gagctccata tgattttaga
    60421 taaaatagac agtccggaaa aagagattct gatcctctac ttttttgaag gatttcgttt
    60481 gaaagaaatt gcgactatct tggatatgaa tgaaaacaca gttaaaacga aaatgtaccg
    60541 ctcattgaat gctttacgtc aaatcctaga atgaaggaga gaagagtgcc atgtattccc
    60601 aaaaagaaaa tttaaaaaaa ctaaagaaaa cttataaaga acaagcaatt cccaaagggc
    60661 ttaaagagga gatccgccag cgttttatta tcgaagagca atcatttata aaagcaaagc
    60721 ggagaaagag acgtctccaa gggttcggag tatctttggc tatcgtaatg attacaacaa
    60781 gtagccttct ttttaatagt caagttcgaa gctttgcaga agatttaccc atcttaggat
    60841 cagtcattga attgatctta ggcgaacgct ttactgatcg atctgaaaaa atcgatattc
    60901 aggtaccgaa gatcaataca caaaatgaga aagaaaataa aacaattcat ggattaaatc
    60961 aaaaatactt tcgcgaagga caagcagatt ttgaaaaagc tgaaaaagaa tatggaaatt
    61021 ttgaaacgga tcattaccaa gttttgggag attatcagaa aatcgtagac gataaccggt
    61081 ttctcgtgat tgaaaggcag atcacccaaa cagcagcaga tagccatgtt gaaaaaagat
    61141 acgacacaat cgacaagaaa aacagtgtcc agctctcact tccactttta ttcaaagatg
    61201 acacctatct atctgttttg acaaaagaag tcaaacggca gatggccgaa caagtcaagg
    61261 aagatccatc taagtactat tggactgagc aagatattca agaagggacg atagaaaaac
    61321 caacacttgt tacgcctaca cgaagttttt atttgaacaa agagcatcaa ttagttttga
    61381 ctttttctca atacgaaatt gctcctggtt atatgggtac accagaattt gtcattccta
    61441 agtccgtgac aaaaacgatt ttggcatcgg aagattattt ggatcattag acgagaagta
    61501 gttgcgacta gcagtttcaa ttttcaagta agtacaagta aataataaga agagaaaaag
    61561 agaaagaaga gatatagtga aaagaaatta tttagttatc agtattattg ctttatccgt
    61621 gcttttgttt ttcgaaatta cttcggtggc ctccttgatc accgaagtaa aaggacaaac
    61681 acactcttcg gagcattcgt cacaatcttt caaacaatca gaacaatcaa ccattaaaag
    61741 ccaatcaact gaaacagcta acgtcaaaga agccattcaa gaatcagcgg aaaaggtaca
    61801 aagtcagtca tcacaatcaa cacaagacag tacaaaaacg agccgagatc cggctgtcaa
    61861 aagcgtcgca atcagctttg atgatggacc aggagcgacc acaacaccgc agcttctgcg
    61921 aatcttaaaa gaaaaaaacg tgcatgccac ttttttcgtc ttaggagaaa atacggcgca
    61981 gcatcctgaa atcgtgaagc agacagctga agcagggcac gagattggca atcacacata
    62041 cgatcatcag aatctggcca ctctttctgc ccagtcgatg acggaggaag tgacgaaagc
    62101 agacactgag atcaaaaaag tcactggcaa aacccccact tttgttcgtc cgccctatgg
    62161 ttctgtcacg aatgtaggag caacgatcat ccagcgaccg atcatcgaat ggtctgttga
    62221 ttcagaagat tggaagacaa gaaatcctga tttgattttg cagaagatcc aagcaacagt
    62281 atacgatggg gcaattattt tattccatga tatctatcct gaaacgattc gtgccgttcc
    62341 tcaagtgatc gattatctga aagagcaagg gtatcgcatc acgacagttg gggatttatt
    62401 agggcatcca acggctgtcg agaactatta tggcagaaac gatcatcggc cagttcagta
    62461 agtgagacaa gtatctttaa taaataacac attttaataa gtagttgaac acataactac
    62521 cttacttgca aaggcttaac aatatctata caggtacaac aaaaaactgt ccaccaattt
    62581 t
//
LOCUS       DAZKKW010000006        60566 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.006, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000006 DAZKKW010000000
VERSION     DAZKKW010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 60566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60566
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.006"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(177..971)
                     /locus_tag="ACG8PO_000334"
     CDS             complement(177..971)
                     /locus_tag="ACG8PO_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HFQ0205692.1"
                     /translation="MSKQCAKCGGKIGLTSYKIKDKQIVCGNCMKKAGYGMTTPYKII
                     RSLNLEDLDKDPSEKRKLNISDHTAQNNHDIGNDRMRTTEEMVEFCLKYGYGKGMTKK
                     WTTHHFNLISDQLNSDEYVIFCFVGLHNYISSTKHDNNYAYALTNKRLIVAQQKMIGN
                     NVQSIILDNLNNISKKRGVLLGTLTIDTLGKVLNVAVDKDTVDRISDSLNEIIYNLKR
                     RNISATNSNTSTISSASEIRKYKELLDDGIITEEEFNKKKQELLDL"
     gene            1617..4424
                     /locus_tag="ACG8PO_000335"
     CDS             1617..4424
                     /locus_tag="ACG8PO_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFQ0205693.1"
                     /translation="MYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNSSKDLNQLVR
                     EGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDTKDIFAKIIG
                     ANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAHHVIDEETEL
                     IVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLDEIHRLPPEG
                     QEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRIPINIKLPAF
                     EKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQLKSNIQLVCA
                     RGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKITVAPNDTIMK
                     IQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFYKDHGFSFNA
                     DSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKKINLGEERHT
                     NDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLRANQASGRIG
                     VVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNVQEVDEGSGV
                     ILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDLDMLHESLKN
                     FRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNKRQETELEVL
                     ALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVILDQLLMESE
                     LEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTTGIGDEEYGF
                     KINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLEDQIKVKVPIE
                     EMYYLLRLVHNQLDKKEYTVP"
     gene            4615..5106
                     /locus_tag="ACG8PO_000336"
     CDS             4615..5106
                     /locus_tag="ACG8PO_000336"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIB"
                     /protein_id="HFQ0205694.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            5209..6198
                     /locus_tag="ACG8PO_000337"
     CDS             5209..6198
                     /locus_tag="ACG8PO_000337"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIB"
                     /protein_id="HFQ0205695.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            6231..7034
                     /locus_tag="ACG8PO_000338"
     CDS             6231..7034
                     /locus_tag="ACG8PO_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HFQ0205696.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            7055..7969
                     /locus_tag="ACG8PO_000339"
     CDS             7055..7969
                     /locus_tag="ACG8PO_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFQ0205697.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            8052..8426
                     /locus_tag="ACG8PO_000340"
     CDS             8052..8426
                     /locus_tag="ACG8PO_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HFQ0205698.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            8565..8810
                     /locus_tag="ACG8PO_000341"
     CDS             8565..8810
                     /locus_tag="ACG8PO_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFQ0205699.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            8982..10046
                     /locus_tag="ACG8PO_000342"
     CDS             8982..10046
                     /locus_tag="ACG8PO_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HFQ0205700.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            10292..10717
                     /locus_tag="ACG8PO_000343"
     CDS             10292..10717
                     /locus_tag="ACG8PO_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFQ0205701.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            10710..11159
                     /gene="nusB"
                     /locus_tag="ACG8PO_000344"
     CDS             10710..11159
                     /gene="nusB"
                     /locus_tag="ACG8PO_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFQ0205702.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            11256..12110
                     /locus_tag="ACG8PO_000345"
     CDS             11256..12110
                     /locus_tag="ACG8PO_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HFQ0205703.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            12111..13448
                     /gene="xseA"
                     /locus_tag="ACG8PO_000346"
     CDS             12111..13448
                     /gene="xseA"
                     /locus_tag="ACG8PO_000346"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFQ0205704.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            13453..13677
                     /locus_tag="ACG8PO_000347"
     CDS             13453..13677
                     /locus_tag="ACG8PO_000347"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFQ0205705.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            13679..14569
                     /locus_tag="ACG8PO_000348"
     CDS             13679..14569
                     /locus_tag="ACG8PO_000348"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFQ0205706.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            14578..15396
                     /locus_tag="ACG8PO_000349"
     CDS             14578..15396
                     /locus_tag="ACG8PO_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HFQ0205707.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            15503..15952
                     /locus_tag="ACG8PO_000350"
     CDS             15503..15952
                     /locus_tag="ACG8PO_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFQ0205708.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            15970..17652
                     /gene="recN"
                     /locus_tag="ACG8PO_000351"
     CDS             15970..17652
                     /gene="recN"
                     /locus_tag="ACG8PO_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFQ0205709.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(17707..17823)
                     /locus_tag="ACG8PO_000352"
     CDS             complement(17707..17823)
                     /locus_tag="ACG8PO_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HFQ0205710.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(17896..18852)
                     /locus_tag="ACG8PO_000353"
     CDS             complement(17896..18852)
                     /locus_tag="ACG8PO_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFQ0205711.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            19023..19391
                     /locus_tag="ACG8PO_000354"
     CDS             19023..19391
                     /locus_tag="ACG8PO_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HFQ0205712.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            19561..19992
                     /gene="mraZ"
                     /locus_tag="ACG8PO_000355"
     CDS             19561..19992
                     /gene="mraZ"
                     /locus_tag="ACG8PO_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HFQ0205713.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            20013..20972
                     /gene="rsmH"
                     /locus_tag="ACG8PO_000356"
     CDS             20013..20972
                     /gene="rsmH"
                     /locus_tag="ACG8PO_000356"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFQ0205714.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLRQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            20977..21378
                     /gene="ftsL"
                     /locus_tag="ACG8PO_000357"
     CDS             20977..21378
                     /gene="ftsL"
                     /locus_tag="ACG8PO_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFQ0205715.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            21379..23571
                     /locus_tag="ACG8PO_000358"
     CDS             21379..23571
                     /locus_tag="ACG8PO_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFQ0205716.1"
                     /translation="MSLKNKIWRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKTGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            23597..24559
                     /gene="mraY"
                     /locus_tag="ACG8PO_000359"
     CDS             23597..24559
                     /gene="mraY"
                     /locus_tag="ACG8PO_000359"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFQ0205717.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            24573..25931
                     /gene="murD"
                     /locus_tag="ACG8PO_000360"
     CDS             24573..25931
                     /gene="murD"
                     /locus_tag="ACG8PO_000360"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFQ0205718.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RL"
     gene            25948..27036
                     /gene="murG"
                     /locus_tag="ACG8PO_000361"
     CDS             25948..27036
                     /gene="murG"
                     /locus_tag="ACG8PO_000361"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFQ0205719.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            27051..28271
                     /locus_tag="ACG8PO_000362"
     CDS             27051..28271
                     /locus_tag="ACG8PO_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HFQ0205720.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNRQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            28427..29752
                     /gene="ftsA"
                     /locus_tag="ACG8PO_000363"
     CDS             28427..29752
                     /gene="ftsA"
                     /locus_tag="ACG8PO_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFQ0205721.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            29775..31016
                     /gene="ftsZ"
                     /locus_tag="ACG8PO_000364"
     CDS             29775..31016
                     /gene="ftsZ"
                     /locus_tag="ACG8PO_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFQ0205722.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            31033..31710
                     /locus_tag="ACG8PO_000365"
     CDS             31033..31710
                     /locus_tag="ACG8PO_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HFQ0205723.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            31724..32323
                     /locus_tag="ACG8PO_000366"
     CDS             31724..32323
                     /locus_tag="ACG8PO_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HFQ0205724.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            32404..32625
                     /locus_tag="ACG8PO_000367"
     CDS             32404..32625
                     /locus_tag="ACG8PO_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HFQ0205725.1"
                     /translation="MVYALLSWFPGGYQSGLGRLLAKICEPYISLFDRLPLRIGPLDF
                     TILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            32674..33456
                     /locus_tag="ACG8PO_000368"
     CDS             32674..33456
                     /locus_tag="ACG8PO_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HFQ0205726.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            33477..34184
                     /locus_tag="ACG8PO_000369"
     CDS             33477..34184
                     /locus_tag="ACG8PO_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HFQ0205727.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            34446..37244
                     /gene="ileS"
                     /locus_tag="ACG8PO_000370"
     CDS             34446..37244
                     /gene="ileS"
                     /locus_tag="ACG8PO_000370"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFQ0205728.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKSYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(37293..38819)
                     /gene="zwf"
                     /locus_tag="ACG8PO_000371"
     CDS             complement(37293..38819)
                     /gene="zwf"
                     /locus_tag="ACG8PO_000371"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFQ0205729.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(38834..39481)
                     /locus_tag="ACG8PO_000372"
     CDS             complement(38834..39481)
                     /locus_tag="ACG8PO_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HFQ0205730.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(39665..39994)
                     /locus_tag="ACG8PO_000373"
     CDS             complement(39665..39994)
                     /locus_tag="ACG8PO_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HFQ0205731.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            40171..40899
                     /gene="sfsA"
                     /locus_tag="ACG8PO_000374"
     CDS             40171..40899
                     /gene="sfsA"
                     /locus_tag="ACG8PO_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HFQ0205732.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            40915..41928
                     /locus_tag="ACG8PO_000375"
     CDS             40915..41928
                     /locus_tag="ACG8PO_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFQ0205733.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            41928..43205
                     /locus_tag="ACG8PO_000376"
     CDS             41928..43205
                     /locus_tag="ACG8PO_000376"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFQ0205734.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            43268..45970
                     /locus_tag="ACG8PO_000377"
     CDS             43268..45970
                     /locus_tag="ACG8PO_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFQ0205735.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            46122..46439
                     /locus_tag="ACG8PO_000378"
     CDS             46122..46439
                     /locus_tag="ACG8PO_000378"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0205736.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            46469..46789
                     /locus_tag="ACG8PO_000379"
     CDS             46469..46789
                     /locus_tag="ACG8PO_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFQ0205737.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            46897..48357
                     /locus_tag="ACG8PO_000380"
     CDS             46897..48357
                     /locus_tag="ACG8PO_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFQ0205738.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGTLPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGPNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            48425..48646
                     /locus_tag="ACG8PO_000381"
     CDS             48425..48646
                     /locus_tag="ACG8PO_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0205739.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            48677..48859
                     /locus_tag="ACG8PO_000382"
     CDS             48677..48859
                     /locus_tag="ACG8PO_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HFQ0205740.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            48859..49272
                     /locus_tag="ACG8PO_000383"
     CDS             48859..49272
                     /locus_tag="ACG8PO_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HFQ0205741.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            49395..50576
                     /locus_tag="ACG8PO_000384"
     CDS             49395..50576
                     /locus_tag="ACG8PO_000384"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0205742.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            complement(50647..50811)
                     /locus_tag="ACG8PO_000385"
     CDS             complement(50647..50811)
                     /locus_tag="ACG8PO_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0205743.1"
                     /translation="MNDKPKEKVYAERLDSSKKNREKITKEAIQDHKKENPKTKENKK
                     FKFTKKEDLL"
     gene            50965..51038
                     /locus_tag="ACG8PO_000386"
     tRNA            50965..51038
                     /locus_tag="ACG8PO_000386"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:50999..51001,aa:Arg,seq:cct)
     gene            complement(51107..52246)
                     /locus_tag="ACG8PO_000387"
     CDS             complement(51107..52246)
                     /locus_tag="ACG8PO_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0205744.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTIDLLKKFKINQKELFENFEIK
                     NPHNFVFFNLKNGLVSSNAVSKYLRKKLKELGIEKQFTLHGLRHTHASILLYQGVNIL
                     SVSKRLGHSSLETTMSTYLHIVRELEDQDKEKINTVFDSLYKNDN"
     gene            complement(52545..53180)
                     /locus_tag="ACG8PO_000388"
     CDS             complement(52545..53180)
                     /locus_tag="ACG8PO_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0205745.1"
                     /translation="MDNSNEKLTKKEYLELIVEGGIGAIPYIGGVVQSLYFGSKNEKR
                     FKRIEKFYKNLNESLENIRDQIPEDVFNLENKDQLIGIFEGINDEVEKSKAQNKIDMY
                     KNLYKNCLLRINNASWDNEEYYLLVLNQLTSIEIQLLACLMSRGNENFTGNISMSGYS
                     QELIDGSLNRLSDFGLLEKTISSIVLGDVGKQNMGYKISHLGIQFINSTLT"
     gene            complement(53293..53928)
                     /locus_tag="ACG8PO_000389"
     CDS             complement(53293..53928)
                     /locus_tag="ACG8PO_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3221 domain-containing protein"
                     /protein_id="HFQ0205746.1"
                     /translation="MDGIFGFTGIVLFIIGLIMLIVRFIKKTNKKTPMILLIIGIIFT
                     TVGFSLSSSNEANDNNKQESSSSDTQTTTSNSSNKSLTQFEEFLETNNHDWGAFLDSY
                     YSITPATEQNTAFTKYISGKTYTFEGTVIESMTTRIAIIADKEYDDKSWNDISATPKV
                     SYVIFAKDLNNADTFTKGDKVTFTGEISSRGSNIEKSYAQWDMINSKVSKK"
     gene            complement(53962..54423)
                     /locus_tag="ACG8PO_000390"
     CDS             complement(53962..54423)
                     /locus_tag="ACG8PO_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4429 domain-containing protein"
                     /protein_id="HFQ0205747.1"
                     /translation="MEKEILIKSPGKTLIKVTDDSISIIRKGFINLVNQGIKGEKTIP
                     FKNISAVQLKKPGMSNGYIQFTLLGGNESRGGILAATKDENTVMFTKKYWNEMENLKK
                     YIEKQQSILDNNSKETQISSADEIKKYKELLDEGIINQEEFDFKKKELLGL"
     gene            complement(54553..54984)
                     /locus_tag="ACG8PO_000391"
     CDS             complement(54553..54984)
                     /locus_tag="ACG8PO_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFQ0205748.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFSASHFEFSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            complement(55002..55322)
                     /locus_tag="ACG8PO_000392"
     CDS             complement(55002..55322)
                     /locus_tag="ACG8PO_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0205749.1"
                     /translation="MNTYEIIKDLAKRKKISIRQLEINFGYSNGYLAKWKNNTPNSDE
                     LSRIADYFDVSVDYLLGREERETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            55621..56397
                     /locus_tag="ACG8PO_000393"
     CDS             55621..56397
                     /locus_tag="ACG8PO_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HFQ0205750.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVASVLGYSNTKDALS
                     RHVDLEDKMGSRITTSGQSREMTIINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATQLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMNQGYTKSHTTEIPK
                     ADGGTKIVMNTKWTQKGRLFIYELLKKEGYYPQMDLEEIG"
     gene            56412..56615
                     /locus_tag="ACG8PO_000394"
     CDS             56412..56615
                     /locus_tag="ACG8PO_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0205751.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            56631..56951
                     /locus_tag="ACG8PO_000395"
     CDS             56631..56951
                     /locus_tag="ACG8PO_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HFQ0205752.1"
                     /translation="MQYLEAKIPIPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRR"
     gene            56956..57126
                     /locus_tag="ACG8PO_000396"
     CDS             56956..57126
                     /locus_tag="ACG8PO_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0205753.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEYRAKKPVGRD"
     gene            complement(57133..57915)
                     /locus_tag="ACG8PO_000397"
     CDS             complement(57133..57915)
                     /locus_tag="ACG8PO_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0205754.1"
                     /translation="MDISDTPYYQKLIASSKLIGETIAPSLDVVNAMQPAIEQAKKLI
                     AIQDTFIASQAILNDSLIQIVEKRNKSIANMIPPSLFAIQKQLESPSFNIAKKMAATM
                     PKPYFSDISKITEKFAVDMSKYDFRIGVATQELEKAFSSEPEVSTKNLLFKDTSNPSD
                     EFEAKFFDILNEQRKVLDDSIKLFEEINTAKNKDITNEPSEKQQASPAFYKDKMWYLE
                     QTGAALIGLVVTEIVNITIGVDPNNTISLALFLRYLLQFLIN"
     gene            57981..58673
                     /locus_tag="ACG8PO_000398"
     CDS             57981..58673
                     /locus_tag="ACG8PO_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFQ0205755.1"
                     /translation="MLKNKEAVTTSLQVAETFRKEHRAVLKAIDDLKDGLAQKYADLF
                     WEDVYIHPQNKQAYRIVYMNRDGFTLAVLGFNNTQKVLEFKLKYIEAFNEMEKAIRKN
                     TAFDTIEDMMIYQLEEMKDVKKDVSMLKNTMRISGQQEFEIKQKGNTKVMEVLGGKES
                     RAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEIDQL
                     NRQQRLFGDENE"
     gene            58851..59192
                     /locus_tag="ACG8PO_000399"
     CDS             58851..59192
                     /locus_tag="ACG8PO_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0205756.1"
                     /translation="MEKELSTLDQYLTDPSWGKSNIKETNNRKIRRNLLTDEELACDQ
                     DDLGNFVSIWDHVYLIHLSRKSKKPEYIYVIEDGLIDALEEYDRDNLIDISYYGSGKK
                     YIAEMEAEFDE"
     gene            59185..59856
                     /locus_tag="ACG8PO_000400"
     CDS             59185..59856
                     /locus_tag="ACG8PO_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HFQ0205757.1"
                     /translation="MSENKGTTTFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            59862..60548
                     /locus_tag="ACG8PO_000401"
     CDS             59862..60548
                     /locus_tag="ACG8PO_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative HNHc nuclease"
                     /protein_id="HFQ0205758.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDIGATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHSEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     ETIKKLRIGG"
BASE COUNT        20179 a        10156 c        12549 g        17682 t
ORIGIN      
        1 ccacacctta taaaaatctt tgacaaatct attcgaagga tcgaatttaa accataattg
       61 tgtttttcct tccattaaca tataaagttc acctcaaaaa gagtatacga acaaatgttt
      121 gtattgtaaa tagaaaaaga tgagctaaat taaatgccca tcttctttga tagatattat
      181 aaatctaata attcttgttt ctttttatta aattcttctt ctgtaatgat tccatcatcc
      241 aatagttctt tatattttct tatttctgaa gctgaggaaa ttgttgatgt attggaattt
      301 gtagcagaaa tatttcttct ttttaaatta taaataattt cgtttaaaga atcacttatc
      361 ctatcaacag tatctttatc aactgcaaca ttcaatactt taccaagagt atcaatagtt
      421 aatgtaccca acagcactcc tcttttttta ctaatattat ttaaattgtc taatataata
      481 ctttgtacat tattgcctat cattttttgt tgagctacaa ttaaacgttt attagttaat
      541 gcgtatgcat aattattgtc atgcttagtt gatgagatat aattatgcaa tcctacaaag
      601 cagaagatta cgtattcgtc actgttaagt tgatctgata ttaagttaaa atgatgagtt
      661 gtccattttt tagtcattcc tttaccgtag ccgtatttaa gacaaaattc taccatctct
      721 tcagtggttc tcattctatc attacctata tcatgattgt tttgtgcagt atgatcagat
      781 atgtttaatt ttcttttttc tgatggatcc ttatctaaat cttctaaatt caaagatctt
      841 attattttat aaggagtagt cataccgtaa ccagcttttt tcatacaatt gccgcaaaca
      901 atttgtttat ctttgatttt atatgaagtt aagccaattt tacctccgca ctttgcacat
      961 tgtttactca cattagttcc tcctagatat cagctttcta ctactattaa cattctatca
     1021 atagtgacca tactttgacc atacttttta gaaagaatag aaaattccaa gatatttaat
     1081 aatagaagaa aagcactaac atcatgggtt cagaagaatt gagagcttat caggaaacag
     1141 tataattgaa gtgtctgttt atccagtagc tcaagaagct taattgaatc ttatgattaa
     1201 agaggttgtg acaagacttt tgtcacagcc ttttttgttt ataaaaataa aagttgtagt
     1261 ggaggctacg aagtttactt tgtcttttta cacttaatat ttttttgcca atttggaatc
     1321 aggatgtttt gataactatc gttttcaatt gatggaagct atattgtttg ctatcagtga
     1381 aataattata tggtaaacag tgttcttttt cttgtttcca ttataaaaat cgtatcggga
     1441 tttttcgttc ttttaaattg aacatttcta tctatccgcg tgttatcaac aaaacaaaat
     1501 caggtaaata gttgacgaaa cagaaataaa tacatagtat tatgttttga agcctaagta
     1561 caatagtcta tagaaaagga gcgataccat tggcaaaacg cattgaccga atttatatgt
     1621 atgtcaaaga aaaaaccgct catttaacaa aggcagaata tgatcaagga gtaacaacac
     1681 aggaaattgc tgaagtgtta gggattcagc gaacaaatag cagtaaagat ctgaatcagc
     1741 tagtgcgcga aggaaaactg ataaagacag acggacgacc agtacgttac atatatcaaa
     1801 cactcgttac gcacgtaaag ccagttacaa aacatgtagt cagttacaaa gaaacgccgg
     1861 tctctgaaga aaaaaataca ctccaaatag atactaagga tatctttgca aaaatcattg
     1921 gagctaacgg cagcatgaaa aattcagtgg aacaggcaaa agctgcgatt ttgtatccgc
     1981 caagaggatt aaattgtttg attacaggac ctaccggatc aggaaagact tattttgcac
     2041 acgcgatgta tcattttgca aaagcgcatc atgtaataga tgaggaaacg gaactgattg
     2101 ttttcaattg tgctgattat gccaataacc ctgagctttt gatgagtcat ttatttgggt
     2161 acgcaaaagg tgcttttaca ggcgcagatg aagaaaaaat ggggattatc gatcaagcgg
     2221 atggcggtat gctgtttttg gatgagattc accggttgcc gcctgaaggt caagaaatga
     2281 tcttttactt tatggatcat ggaacttaca gtcgtcttgg ggaaacaacg aaatcccatg
     2341 aagcaaatgt acggatcgta ggtgcgacca cagaagaccc aggttcttct ctattagaaa
     2401 cttttgttcg gcgtatccca atcaatatca aacttccggc ttttgaaaaa cgtcctgcaa
     2461 atgagaaagt cgatttagta aaaatcatga ttgcccacga agcaaaccgc acgcagcgaa
     2521 agatttcttt gacagaagat gtagcaaaag cattgatcgg cagtgtgacc tatggaaata
     2581 taggacaatt gaagtcaaat atccagttag tatgtgcacg aggatttcta aatcatatgc
     2641 aatcacccga aatctcgatc acaatcgatg atttgacaga agggattcgt agcggattga
     2701 ttcaattagc aagcaaccgc agtgcaatgg cagagctttc taaaatattg gagccaaaga
     2761 taactgtagc accaaatgat acgatcatga aaatccaatc ggactcctat gagttacctt
     2821 ataatctata tgatatcatc ggagataaag cagctttact gaagtcggat ggtttggatc
     2881 aagaagcaat caatcatttc atttctacag atatcaacat ccatctgaag tctttctata
     2941 aagaccatgg tttttctttt aatgcagaca gcaaattagc agaatttgta gatccgaaaa
     3001 tcatagaagt gaccaatcag atttatgtaa tggtcaaaag atctttacca tatgagtttc
     3061 agcaaaactt tatttatgct atgagcttgc atatcagttc ttttttgaaa aaaatcaact
     3121 taggagaaga acggcatacg aatgataaca tccgtgagat ggcttttgat tttccagatg
     3181 aatatgctgt tgcgaaagaa gtgcgtagtt atatcgaaac gtattttcaa gtggagattc
     3241 cagaaagtga agactattac ttgaccgttc tactcgtctc gttacgggca aatcaagcaa
     3301 gcggcagaat cggtgtagtc gttgcagctc acggaaacag cacagcaagc agtatggtcc
     3361 aagttgtcca acagttgtta gatgcagaga acgtacgagc agtagacatg cctttggata
     3421 tggatccgaa aacagcattg gaccgaattg aacgcaatgt acaagaagta gatgaaggaa
     3481 gcggggtcat actcttagta gatatggggt cccttgcgtc gtttaattcg caaattcaaa
     3541 gagacactgg aattccagta cgtactgttg atatggtaac aacttctcta gttttggaaa
     3601 ctgtacgaaa ggtctcagta ttagggacag atctagatat gttgcatgaa tcattgaaaa
     3661 attttcgagg ttacgcagaa atatcttctg tagaatctgt tcaggaagca aaacctattc
     3721 ataagaaaaa agctgttttg gctatctgcg catctggcga aggtacagca caaagaatca
     3781 aagaattgat cgaacgagcg gtaaacaagc gtcaagaaac agaattagaa gttctagctt
     3841 tgtcgattgt ggaagtgaaa gaagaactgc cagttatcca aaaagaatat cagatcatag
     3901 ctactactgg gattactgat ttgaatatcc aagccccatt tattccttta gaacgtttta
     3961 ttgatcaaaa tatcgaagtc attttggatc agttgctgat ggaatcagaa ttggaggaaa
     4021 ctgaattcat ctccctagat gaagagtcag ctaaaaatac gtgtgttgag tttatctctg
     4081 ataacttcat ctttattaat ggtagtaagt taatcgatcc aatgtggcaa ttttctacac
     4141 aaatctctca gacgacggga atcggcgatg aagaatatgg attcaaaatc aacctagtga
     4201 tgcacacagc aggaatgatc gaacgtatta ttcggaatga accattgact gtggaagaaa
     4261 acgaattgac aaatacaacg aatgatcctc tttatagcca attggcagcc tcagttgttc
     4321 tgttggaaga tcagataaaa gtaaaagtgc cgatagaaga aatgtattat ctgctacgtc
     4381 tagtccataa tcagttagat aaaaaagaat acactgtgcc atgaatacac aaaaacgata
     4441 cacttttata gtgtatcgtt tttgtgtatt ggttaagaat cctttaaatt caattagtgt
     4501 atcgtaaaaa agttggcacg ctttttgctt atagtagtgt gtgctaggag gtggttgttc
     4561 acacagaaat gataggggtt tctgtgaagt agtgaggaag aaggagtttt gcagatggat
     4621 attcgattgg cacgtataga tgatcgttta atccacgggc aagtcgcaac agtttggtcg
     4681 aagcaaacag gtgttgaacg tatcattgtg atcagcgatg tagtcgctca agataaattg
     4741 agaaagtttc tactaaaaga agctgcaccg ccggggatca cagcaaatgt aataaccgtg
     4801 cagaaaatga ttcaaatcta tcatagtgga ttattatcac gagcaaaagt gatgctttta
     4861 ttcaccaatc cccaagatgt tgaagcggtt gtccgctgcg gtgtacagtt gaagtcgttg
     4921 aatatcggcg gtatgagttt tactgaaggc aaatcaatga ttaccaattt tgtttcagta
     4981 aatcaacaag atattgattc ctttcgttac ttgaacagtc aagaagtgga gttagagatt
     5041 cgaaaagtac ctgctgacag gaaaatcctt ttgatggaac tactagcgaa aatcaaaaag
     5101 gtgtagcaag aatggttgtt ggaaaaggtc aaataattct gttgtaatgg ttagcaatta
     5161 ttaatatttt tgaatttgtt acaacaaaag taaataggag gtataaatat ggtagggatt
     5221 atcctagcaa gtcatggaca atttgctgaa ggaatcttac aatccggttc aatgatcttt
     5281 ggcgaacaag aaaacgtcaa agccgtgatt ttgaaaccaa gtgaaggtcc agatgacttg
     5341 agagccaaat tggaagaagc ggttgcttct tttgataatc aagacgaagt gttattctta
     5401 gttgatttat ggggcggaac accgttcaat caatcaaaca cattattcga agagcacaaa
     5461 gataaatggg caatcgtcag cggtttgaat ttaccaatgt tgattgaagc ctacgcatca
     5521 cgcttttcaa tggaatctgc tcatgaaatt gcagcacaca tcattgaaac agcaaaagac
     5581 ggtgtaaaag taaaaccgga agaattagaa ccggcagaag caccgaaagc agcagttgaa
     5641 gacgcacaac ctaaaggagc acttccagaa ggaactgtgg tcggagacgg aaaaatcaaa
     5701 tatgtattag cacgtgttga ctcacgtctg ttacatggac aagttgcgac agcttggaca
     5761 aaagcagtgc aaccaaaccg tattatcgtt gtttcagatg cagtatcaaa ggatgatctg
     5821 cgtaagagac taatcgaaca agctgctcct ccaggtgtaa aagcaaatgt tattcctatt
     5881 agcaaaatga tcgaagtagc aaaagaccct cgttttggaa ataccaaagc attgctttta
     5941 tttgaaaatc ctgaagacgt attgacagct gttgaaggtg gcgtagacat caaagaatta
     6001 aatgttgggt caatggcgca ttcggtcggt aaagtcgttg taagtaaagt attgtctatg
     6061 ggacaagaag acgttgaagc gtttgagaag cttgaacaaa aaggtgtgaa attcgatgta
     6121 cgtaaagtac ctaatgattc acgcgataac atggatgata ttttgaaaaa agcaaaagct
     6181 gaattagcaa aagcgtaata aataatatta aaatttatag gaggcttatc atgtctatta
     6241 tttcaataat tttagtcgta ttagttgcct ttctagctgg tatggaaggt attcttgatg
     6301 aatttcaatt tcatcaacca ctagtggcat gtacattgat cggtttagtt accggtaacc
     6361 tcgaagctgg tatcgtactt ggcggtacgc ttcagatgat cgctttaggt tgggcaaata
     6421 tcggtgccgc tgtagcacca gatgctgcat tagcatcagt agcatcagca attattttag
     6481 ttttaggtgg acaaggtgtt aaaggtgttc cttctgcgat cgcgattgcc gttcctttgg
     6541 ctgttgcagg gttgttctta acaatgatcg tacgtacaat cgctgttcca atcgttcatt
     6601 tgatggatgc ggctgctgaa gaaggaaaca tccgtaaggt tgaaatgtgg cacatcattg
     6661 ctgtatgttt acaaggggtt cgtatcgcgg tcccagcagc agcgttgcta tttatcccag
     6721 cacaaactgt gcaatcattc cttgaatcaa tgccagcatg gttaacagat ggtatggcta
     6781 tcggtggggg aatggtcgta gctgtaggtt acgctttggt aatcaacatg atggctacaa
     6841 aagaagtatg gccattcttc gttattggtt tcgttgtagc tgcaatttct caattaacat
     6901 tgattgctct aggtgcttta ggtgttgcat tagctcttat ttacttgaac ctttctaaaa
     6961 tgggtggttc atcaaataat ggaggcggaa gcaactctgg tgacccttta ggcgacattt
     7021 tgaatgacta ttaatcttga aggaggaaaa taaaatggca gaagaaaaaa tcaaattatc
     7081 aaaaaaagat cgtttagctg ttgcgtggcg ctctaccttc attcaaggtt catggaacta
     7141 cgaacgtatg caaaacggtg gctgggcatt ttctatgatt ccagcaatca aaaaattata
     7201 caaaacaaaa gaagaacgtt ctgcagcatt aaaacgtcac ttggaattct ttaatacaca
     7261 cccatacatt gcttctccaa ttttaggggt tacattggct ctggaagaag aacgtgcgaa
     7321 tggcgcgcct gttgatgatg tagcgattca aggggttaaa gtaggtatga tgggtccttt
     7381 agctggtgtt ggtgacccag tgttctggtt tacagtacgt ccaatgctag gtgcactagg
     7441 tgcttcatta gctatgggag gaaatatcct tggaccaatc atcttcttct taggttggaa
     7501 cattattcgt tgggcattca tgtggtatac acaagaattt ggctacaaag ctggttctaa
     7561 aattactgat gacctttctg gcggattgtt acaagacgta acaaaaggtg cttcaatttt
     7621 agggatgttc gtattagctg cccttgtaca aagatgggta tcaatcaaat tcttaccagt
     7681 tgtatcaact gtaaaattag ataaaggtgc ttacatcgaa tgggataaat tgccttcagg
     7741 tggtgaagga atcaagagtg catttgaaca agttaataat ggcttagccc tttctcctga
     7801 aaaggtaaca acattacaag acaacttaga ccaattgatt cctggtttag ctgcattagg
     7861 attaacattg ttatgtatgt ggttgcttaa gaaaaaggtt tctccaatcg ttatcatcct
     7921 tggtttattc gtagtcggtg ttgttggaca cgtcatcgga cttttataat ccataaaaat
     7981 atagctcggg cgttgtcccg agcttttttt atatagtata ctgttcataa acaaatatct
     8041 ggagggagaa aatggttcaa tcaatcaata caaaagtaga cttggtaatt gatgctactt
     8101 cgtttacggg tctgacagat tacgggcaaa tcatgatagg tgataatggt tttgagtttt
     8161 accatgcgcg agatagacgc aaatatatcc aaattccatg ggaagaagtg gattatgtac
     8221 tagcatcagt catgctaaag ggaaaatgga ttcctcgata tgccattcaa acgaaaaaaa
     8281 atggtacata cacattttct tccaaagaac ctaaaaaagt gcttcgtgcg atcagagaat
     8341 atgtagatga agatagaatg gtacaatcat tgagtttctt tgatgtagtg aaacgtggta
     8401 ttcgtagtat gtttaaacgc aagtaaaaaa taaagaaaac ttctaagagg aggttttctt
     8461 tattttttta ctttgcagaa gaaaatatga aatagcaaaa ccaattattt gtccccttta
     8521 aatcccatat gctaaaattg attagcaaag aaggaggtta tattatgagt tggttatgga
     8581 cattgattgt tggagccgtg atcggagcaa ttgcaggtgc gatcaccagt aaaggaaatt
     8641 cgatgggctg tgtaacgaat atcattgctg gactagtcgg ttcaagtatt ggacaagcga
     8701 ttttcggcga atggggacca aaagctgcag gtatggcact tattccatct attttaggcg
     8761 cagtgatttt ggtagcagtt gtctcatttt tctttggaaa acgatcataa gtttttacaa
     8821 aatagtaaac taaaaggagt tgcagcgaga ctgtgactcc tttttggctt tatttattta
     8881 aaataaaaag aagttttccg gaaattttta tttaactgtc gtgtacgaag aaaagtttgc
     8941 tataatggaa aaaagcgtgc aaagaaagag ggagtttcat tatgatgcta cgtgtagaaa
     9001 aattgcgcaa aaaaatgcaa gaagaaaatt tggattcatt tttggttacc agtccgtaca
     9061 atcttcgtta cttaacgaac tttacaggta cgacaggatt agctgttatt actttggaaa
     9121 aagcattttt tattactgat tttcgttata ccgagcaggc ggcagcgcag gctcaagggt
     9181 ttgaaattat taaaaatgtc ggacctattt ttgatgaggt agcaaactta gtacgcaaag
     9241 aagaactgca ctcattagct tttgaggaaa caacagtctc atttttagaa tattcggttt
     9301 tagaagaaat catcgatgcg gaattagtac ctgtttcaca gatgatcgaa gaactcagag
     9361 aagtgaaaga tgaagaagaa atcgctatta tcgaaaaagc atgtcatatt gcggatatgg
     9421 catatgatca catcctgaaa atgatccaac cgggcatgac tgagattgaa gtcgcaaatc
     9481 aactagactt ttatatgcgt tctcttggcg cttcaggtgt ttcttttgat acgatcgttg
     9541 ctagcggatt gcgttcggct atgccgcatg gtgtagcaag caaaaaaatc atcgaacaag
     9601 gtgacttgat cacattggac tttggttgct attacgaagg gtacgtttcc gatatgacac
     9661 gtacattcgc tattggtgat cctggggaaa aattaaaaga aatttatcaa atagtcttag
     9721 atgctcaatt agcagttatt gatgctgcaa aaccaggaat gactggtgtc caattagatg
     9781 cagttgctag agattatatt tcaaaacatg gttacgggga agcatttgga cattctacag
     9841 gacatgggat cggtttagag atccacgaag gaccaaacgt ttcttctcgt gcagaaaagc
     9901 aatttgtacc aggaaatgtg atcactgatg aaccagggat ctacttacca ggaatcggtg
     9961 gagtacgtat tgaggatgac ttgttgatca cagaagatgg aaatcgtgtg ttgactcgtt
    10021 caccaaaaga attgatcatc ttatagtttc atcaatagaa gaagtaagcg taaaaatcag
    10081 tgttcacaga ctttgttata gaaaatcact cgatttaatg atacactata gaagaacttt
    10141 taagctgtaa gagataataa agttgggtgt tttctccaat tcatagcagt ttgcagctga
    10201 tcggcggcag ttttcttacc acgtgatcac tatgccgcat catagagaat tcggatgtat
    10261 ctcgtgctta ggcaaaggga ggaataaata aatggcagat gaaaaaaatt tgattttaaa
    10321 cgcaaaccaa gatttaggag agatcgtgat cgctcctgaa gtcatcgaag taattatcgg
    10381 gattgcagct tctaaagtag aaggcgtata cggaatgcgc ggaacttttg ccagcaatgt
    10441 cactgaatta ttaggacgtg cagctcacgg aaaaggtgtt tacttagcaa acgaggaaga
    10501 tggcttgaaa gtagatcttt actgttatct agaatatggt gtttcagttc ctaaagtagc
    10561 aatggaaatg caggaacggg tcagccagca agtccttttc atgacggata tcgatttatc
    10621 tgaagtcaat atccatgtag tagctgttgt tcctgaaaaa ttagaacaac caacaatcga
    10681 agaacttttt gaagacgaag aggaagagaa tgaataaaga attatcaaga cacgagattc
    10741 gggaaatggc attgcaggct ttatttccat tagacttcaa tgcagaccta acaaaagaag
    10801 atgcgatttt caacgcaatc gaattagacc accgcgacat gatcaacgaa gatgaatcag
    10861 agtttgttcc tgtttatctt gatacgctag ttggcggtgt ctgtgcaaaa aaagatgaat
    10921 tggataaagt gattgaaaaa catttaaaaa acaattggca catcaatcgg atctcaaaaa
    10981 tggatttggt gatcttgcgt atcgcgattt ttgaaatgac ttatgtgaca gatgtaccag
    11041 cggctgttgc tttaaatgaa gcaatcgagt tggcaaaaac atttagtgat gaccgttcac
    11101 gtaaattcgt caacggtgtt ttatccaatg tattgaaaga attagaagct ggggcgtaag
    11161 tcctagcttt tttctatgaa tcatcataaa cctgttataa atcgggaaaa ttctatggta
    11221 gaatgaaaac acaaaaaata tctgggagtg gttagatggc agaattgatt aacggaaaag
    11281 aattagctga aaaaatgcag gcagagattg ctgaaaaaat ccaaaaattg aaagataatg
    11341 gtatccatcc cggattagtc gttttattgg taggggaaaa tccagccagc caaatctatg
    11401 taagaaataa agaacgtgta gcaaaagaaa tcggaatcca ttcattggta gagcgttatc
    11461 ctgagacgat ttctgaagac gaactacttg cagaaattga aaaatacaat caagatccta
    11521 gttttcatgg tatattggtc caattaccat tgccaaagca tatagatgaa gaaaagatcc
    11581 tgttagctat ccgccctgaa aaagacgtag acggttttca tccgatgaat ttaggcagac
    11641 tccttgcggg taaaccggac aagattcctt gtactccata cggcatcatg aaaatgtttg
    11701 aagcttattc tatcgatccg gcgggtaaac gagcagtagt tatcggacga agcaatatcg
    11761 tcggtaaacc gatggctcag ctactcttga tggcagatgc aactgttact gttgctcatt
    11821 ctaaaaccga aaatctggct gaactggcac aagaagcaga tattttagtc gtagcaatag
    11881 gacgtggaca ttttgtcaca aaagaattcg taaaaccagg tgctgtagtg atagatgtag
    11941 ggatgaatcg tgatgctaac ggtaagctga tcggtgatgt gaaatttgat gaagtggagc
    12001 ctattgcagg ctggatcact cctgtgccaa agggtgtagg gccaatgact atcacgatgc
    12061 tgatgtatca aaccgtaaaa tctgccgaag cttttggagc aggtgaatag atggcacaag
    12121 aatatttaac tgtcactaca ttaacgaagt atttgaaaag aaaatttgat gccgatcctt
    12181 atttagaacg cgtatattta acaggcgaaa tatcgaattt ccgtttacgt gccaatgccc
    12241 atcaatattt cagcttaaaa gatgaccatg caaaaatatc tgctattatg ttcaaaggag
    12301 cattccaaaa attacgattc cagccaaaag aaggaatgaa agtccttgtc gttgggcgta
    12361 tttcattata tgaagcggga ggttcttatc aaatctatgt agagcacatg gagcctgatg
    12421 gaataggtgc tttatatcaa gaattggaag aacgaaaaga aaagctgatg aaggaaggcc
    12481 tttttcaagg gcctaaaaaa atgctgccac gtttcccaaa gcgaatcgcg gtccttacca
    12541 gtccaagcgg agcggttatc cgcgatatca tcacgacggt gaaaagaaga tatccaattg
    12601 tccaattggt tttgttccca acagttgtac aaggaaacca agcagcggac aatgttgtca
    12661 ggaatatcca gagagtagaa gagatgggca atttcgatac aatgatcatc ggtcgtgggg
    12721 gtggttcgat tgaggattta tggccgttta atgaagagcg ggttgctcgg gcgatttatc
    12781 aagctaatac gccggtgatt tcatctgtag gacatgaaac agataccacg atcgctgatt
    12841 tagtagcaga tgttcgggct gccactccaa cagcagctgc agaattagct gttcctgttt
    12901 tgtcagaaga gttgatgcgc attcaagaac ggcaagctag attgcagcag gcgtttgtac
    12961 gccagatcca gcgcaaacaa gaacggttcg agcgtgctcg acgttcctat gtatttcggc
    13021 agcctgaaag attgtatgaa ggtcagacca tcaaattaga ccagttgaaa cagcgattat
    13081 atcaagctac acagcaaatt tatcatgcga aagaaaaaca aacgatcgca ttaagccacc
    13141 aattggaaca agtggctccg ctttatcgag ttaaagcagc tcgccaagaa actgcttatc
    13201 tagaaaaacg cttagaagag aaaatccaac aatatatgaa acaacagcag cagcgttttg
    13261 ctcaagctgt tcaatctctt gatttgttga gtccattgaa aatcatggga agaggctaca
    13321 gctatacgac gttaaatgat caagtagtaa aaagtgtcac cgagattcat caaggggatt
    13381 tgctgacgat ccattatcaa gatggaatcg ttaaaggaca agtacaagag atagacaaag
    13441 gagaataaaa atatggaaaa tccaactttt gaagagtcac tgcaagaatt agaaaaaatt
    13501 gtcatgcagt tagaacaagg agacgttcca ttagaatcag ctctagattc gtttaaacga
    13561 ggaatggaat taagcaaaca ttgccaagat acattagcta aagcagaaga gaccttgacg
    13621 aaaatgatga cagaatcaaa tgaagaagtt gattttgacg gaaacgagga aagctgagat
    13681 gagattaact gacttttcag ctattcatct acctgctgta gaaaaagaaa tcttgtcttt
    13741 tttagatgag catacaacgg atagaggact ctatgatgcg atgagttatt cagtgaaagc
    13801 aggcgggaaa agaatacgtc ccttattact tttaacggcc gttgcttcct ttgatgagcc
    13861 tattgatgtt cccgtctatc aagtagcagc tgctttagaa atggtgcata cgtattcctt
    13921 gatccacgat gatttgcctg cgatggacaa tgatgatctt cgtagaggaa aacctaccaa
    13981 tcataaagta ttcggcgaag cattagctat attggccgga gatggtttgc ttacaggagc
    14041 attccagcta atcagtatgg cacacttagg taattctcct aagcttctgc tgcttcagca
    14101 attagctgtt tgtgcgggat cacaaggtat ggttgctggg caagctgcag atatcgaggg
    14161 cgaatcgaaa aagctttctt tagaagaatt agcatttatt catgaaagaa agacagggca
    14221 tttgattcgt tatgccttgc tggctggtgg catattagcg aagcaacctg aagaaatctt
    14281 attgctactt caacgattag ccgaacattt aggtttagct tttcagataa gagatgatct
    14341 actcgatgtg attggtacaa cgaaaacatt agggaaaaca gctggtaaag atgaacgtat
    14401 ggaaaaaaat acttacccgc gtttattagg cctagaaaag acacgtgagg cgttggagat
    14461 tgagctgtta tccgccaaca agatcataga caaattagaa gaggacgttt tttcttttga
    14521 tggcgggctg atgcgccaga tgatcaagca atttgatgtg gagaaatgag ggaaagaatg
    14581 gaaaaagaac gcgtagatgt attagcggtc aaacaaggat tatttgaaac aagagaacaa
    14641 gccaaacgtt cagtgatggc tggtttgatt tacaacgaaa aaaacgagcg gtttgataaa
    14701 ccgggagaaa aaattccagt gagtagtgaa ttaagagtca aaggtaaaaa acttccttat
    14761 gtttctcgag ggggattaaa attagaaaaa gcactcaaag catttgatct atctgttgaa
    14821 ggaaaaacat tgttagatat tggtgcatcc actggcggat ttactgatgc agctttgcaa
    14881 aatggcgcaa aaatgagtta tgctttagac gttggctaca atcagctagc ttggaaaata
    14941 agacaagatc ctagagtcgt cgtaatggaa agagtgaatt ttcgttatgc aaaacccgaa
    15001 gactttacct taggaattcc agaagttgct gttatcgatg tttcttttat ttcactaaaa
    15061 ctgatgctgc ctccattaca tgctatttta aaagaagagg gagaagtgat tgctttaatc
    15121 aaaccccaat tcgaagcagg cagagaagct gtagggaaaa acggaattgt tcgtgaccct
    15181 cagacccatc aaaaagtttt agaagacatt ttgtcatttg cggcacaagg tggatatgat
    15241 gtgctggaat taagttattc tccgatcacg gggggagaag gaaatatcga gttcttggct
    15301 cacttgagga aagtacccga aagcggaaca atcaattcag ctattaatat ggcagaagtg
    15361 gtttcaaatg cacacgaaca gtttgaccat aaataaattg taactgtgaa aatcaagtga
    15421 gaagtatgcg gattcttctt tattatacca aggaaataag ctatcattga ttaagaagaa
    15481 tatgctaagg agcgaagacg taatgagaaa aaaagatcgg catcgtttga ttactcgatt
    15541 gttgagtgag caagatatcc gaaagcagga agaatttgtt gaaatcctga aaagcaaagg
    15601 aatatctgtt actcaagcta cgatttccag agatataaag gaactgaaac tgatcaaggt
    15661 accggcttca gatggcggct accgttatag cataccagct gaaacaaacg aagatgtaaa
    15721 tactaaattg gaaaaactat taaaagacgc tttcgtggca gttgaccaaa tggaaaaatt
    15781 tgttattttg aaaacattgc cgggaaatgc ttcggcagca gcaaatttga ttgataaacg
    15841 ttacaagaaa gaattatttt cgattatcaa tgatgacgat aatgttttga tgattacacg
    15901 aactgaagac caggcagttt tattgaagaa agacttcctg cattaccttt aagttgagga
    15961 gcgaatgtga tgctacaaga aatcagtatt actaactttg cgattattcc ggaattgcgg
    16021 ctttcttttc acgaaggaat gaccgcgctg accggagaga ctggtgcggg taaatccatt
    16081 attatcgatg ctttaggtct gttagcagga ggaagaggtt caagcgatta tatacgtcaa
    16141 ggagcagaaa aatgtgtgct tgaagggttg tttgaactac caaaacaaga aggattctct
    16201 gaactgatgg ttgaattagg gatcgaaaca gatgaggaca atctgattgt tcgacgggat
    16261 atgtccttaa ctggaaaaaa tgtttgtcga gtgaatggtc atattatcac attagctaat
    16321 ctgagaagaa tcggcagtta tttagtagat atccaaggac aaaacgaaca tcaggaactg
    16381 ctccagcctg aatcacatct tgctttattg gatcgttttg gagatgtagc tttccaacag
    16441 aagaaaaaga gttatcagca agaatatatc tcttatcgtg agctggaaaa aagggtacga
    16501 aaagtgcagc aaaatgaaaa atcctatgtt cagagaatag atatgctcca tttccagcaa
    16561 gaagaaatag ctgctgctga tttgcaagtc ggagaagaag agaaattaaa agaagaaaga
    16621 gaaaaactta gcaattatca aaaaattgct gatggattag ctgcaggtta cggagcactg
    16681 acgaataatg aacaaaacag tgtagatggt gttggcttgg ccgtttcaga actgcaaggg
    16741 atcgcccatc tagatgtgga gtatgaagca atttatgaaa atatccaaag tgcttactat
    16801 ttgctgcaag atgcaatcgg cgatatgagt cgtcagattg atttattgga gttagatgag
    16861 agtcgtttag aagaagttac gcaacgttta gaattaatcc gacagttgaa acgtaaatac
    16921 ggcgaatcca ttgagtcaat attggcttac tatgacgaaa tcaccgaaga gctagcttct
    16981 tctgattttt cagaaggtca attggacaaa atgaaagagg aactagagca aaaagagctt
    17041 ctacttcagc aacaagccgc agatcttcat gaagcgcgta aaaaaatcgc aaaagaattg
    17101 gaaaaatcca ttctgcatga attgaaaagt ctgtatatgg aaaacactga gtttgaggta
    17161 cgtttcttaa aagaagaaaa tagacaattg aatcatgatg gatttgacca aatcgaattt
    17221 tacattacga ccaatccagg agagccactg aagcctttgg tgaaagtcgc ttctggtggc
    17281 gaactgtctc ggatgttgct tgctttgaaa actatttttt cttctgagca aggtgtgaca
    17341 agtattattt ttgatgaagt ggatacaggt gtgagcggaa gagttgcaca ggccatcgca
    17401 gataaaattt ctcaaatatc gaaatactct caagttttgt gcatcactca tctgcctcaa
    17461 gttgctgcag tcgccgacta tcaatattat attgtgaaag cagtgattga tggacgtacg
    17521 caaacttctg tttcagagtt gaaaacaaaa gaaagagaag aagaaattgc acgtatgctg
    17581 gctggaagtg agattacaaa actcacactc gaacatgcaa aagaactatt gaaactcgca
    17641 aaaaggacct gagccacctc gcagtggttc aggtcctttt cagcagctga tagctatatt
    17701 ttatttttac atgacactca ttacagcagt caatacaaca gatccaatcg ttaaaatcag
    17761 cattgtccaa atgacaaatt ttgttacttt agcaaaagta cttgtttgtt ttttttcacg
    17821 cattgttttc aacctcgtca ttaattagtt gattcaagtg tacaatcttt tttccatttc
    17881 cacaagaggg cttttttata aaaacttccc tttttttaaa taataggtag ttacgataca
    17941 aataaataat gttaatagaa ctagccatat aaaggcatca tccctttcag caataggtaa
    18001 cttaatgttc attccataga tactgccgac gattgtcggt atagttaaaa taatcgttaa
    18061 agaagttaaa attttcataa tattattcag attattcgat acgattgctg aaaaagtctg
    18121 attcatttgt tctactagtt ttaattgaat gcgagaagta gtcattgcct gctctgtttc
    18181 caccaataca tcacgtaaat gatttttcaa agcatgattt tcttggaaaa gtttattttg
    18241 cgctagttct ttcaatgcat ctaaattagc agaagtcgct tctctgaaat aaacgaggct
    18301 tttttggata tccatgattt gataaagttg tttgttttca gtggatactt gaagtttggc
    18361 ttccaaagta tcgcattgag attgaagcgt agctaaataa ctgttgtaag agagtgtgac
    18421 ctgccaaatg aattgaaaga agatattgag attaggcgaa atatcacttt ttggaaaggt
    18481 ctggctaaag actttcttta aaaacagtgg ttcatgattc gtaattgtga taagcttctt
    18541 tttgggcgtc aaaataatag agagcgggta tgtatccact tgaaaataac cgctaggact
    18601 tgttttagca aaaggatatt gtaacaataa taacgctggc tttgtcaact ttgattgatt
    18661 gaattcttct gtacgggaat tttctgcatc gtccaatatg gaagtgatgt aatctgacgg
    18721 aatttcaaat tgatgtgtca gctgatgaat ctcttcttga ttaggacgtt ctacacaata
    18781 ccagatcgtt tggtcagttt cctttccggt attacgcagt ttctcattat ctaatgtaaa
    18841 atatttgatc atgattttcc tcctgttgcc tgtttctttt attataacgg atagggaatt
    18901 ggaatacatg gaatgtgttt gtaagatata gaatataact gcataaattt aaataaaatg
    18961 aaacagttgt gtaaagtata tacagtatac ttgaagcaac gataaaaaag gagtttacgt
    19021 tcatgggcac attcacacca cttatcttgt tttggtatat ttacccggta atcgttttgt
    19081 ttggttgcca atttcttgta aaattatttt cgttaaatcg tcgttttaaa ataaaagccc
    19141 ctgatttggc gattcctttt ttgtggggcg gtatgcatgc attgtctaga aatacaggga
    19201 caatttctat cttaccgttt ttctttattt ctgttttgct aatgggaatt tgtattgctg
    19261 ttttccaagc atattattat gaagaaatta tttatccgcg ttatttcaag atgacttggc
    19321 gtttggtttt tctgttgaca atcgtcttgt acgctgtctt gatcattgtg aacatacttt
    19381 cttacctatg aaaaaaatag aaaagctgta aaaagtcttt taaaaggctt tttgcagctt
    19441 gatttttaaa aaaatgttta tgaataaaaa aagttgttcc cggatttccc agaaaaggtg
    19501 gtagaaagtg ggggattgtg gtagactaat tttagttagt ggaggaatgg ggggctttca
    19561 atgttgatgg gtgaattcca acataatatt gatgcaaaag gacgtttaat cgtgccctca
    19621 aaacttcgtg aagaacttgg cgaaaagttt gttctgacac gcggattgga tggctgtctt
    19681 ttcggctatc cgatgagcga atgggaaaat ctggaagcta aactgaacga aatgccgttg
    19741 gctaaaaaag acgcacgtac ttttgtgcga ttcttctatt cggctgccac tgaatgcgaa
    19801 cttgataaac aaggacgaat caatataccc agcacgttaa gaaattatgc agctttgaca
    19861 aaaggttgcg tagtcatcgg tgtatctaac cgaattgaaa tttgggatga gacacgatgg
    19921 caagattttt ctgcagcagc agaagagaac tttgacgaaa ttgctgaaag tatgattgat
    19981 ttcggactat aagaatagga agaggctgac taatgactga aacatttcaa cactatacag
    20041 tcatgttgaa agaaacggta gatggattga acatcaaacc ggatggcgtt tatgtagact
    20101 gtacactcgg cggtgccgga catagtgaat atttattaag acaactaagt tctaaagggc
    20161 atctgtatgc gtttgaccaa gatcaaaaag caatcgatca cgccaagctg cgtttagctt
    20221 cttatgtcga acaaggtatg gtcacattta tcaaagcgaa cttcagagaa ttagaagaac
    20281 gcttgagtga gcatgtagat aaggtagacg gcattttgta tgatctaggg gtttcctctc
    20341 ctcagctaga tgaagcagaa cgtggattta gttatcatca agatgcaccg ttggatatgc
    20401 gcatggatca gtcagctcct ttatccgctt atcacgtaat taatgaatat tcctaccatg
    20461 aattagtaaa gattttcttc cgctatggcg aggagaaatt ttccaaacaa attgctcgtg
    20521 aaatcgaacg agtacgaaaa acaaaaccaa tccaaacaac tggtgagctt gtcgaaatca
    20581 ttaaacaagt catcccagct cccgcaagaa gaaaaggcgg gcatcctgca aaaagaattt
    20641 ttcaagctgt gcggattgca gtaaatgacg aactcggtgc cgaagaggcc tctttagaac
    20701 aggcaatccg gttattgaaa gtcaatggaa gaatcagcgt aatcacattc cattcattgg
    20761 aagacagaat tgtcaaaagt atgtttaaag aatatagtac ggtacaagat ctaccaccgg
    20821 gacttcctgt catacctgac gaattccaac cagaactgaa agtcattact cgtaaaccaa
    20881 tccttccttc agaggaagag ctaacagaaa acaatcgatc acgcagtgca aagttacgta
    20941 ttgcagaaaa aataagaatg aaagaggagt agaatgatgg cagaattaaa gaaaatgcaa
    21001 gattacgaat atgatttacc tgaattggtg gatcagccgg aacaacaaat gcctgattct
    21061 tcttcgaatc aagaaatcat tcttccacaa tcaccggcaa aacgattaaa gcagatttct
    21121 gctttggaaa aaattgctgt ggcaagcgtg gcgattgcta ttgttgcttt atgtgtttta
    21181 acagttatgc tgcgcacaaa tatcagcgga gctgaaaaag aaatcacgtc aatacaaaca
    21241 gaaatcaatg ataaaaagca agaaaaaacg agtttagaac aagaaaaaaa cgaactttct
    21301 cgtactgaac ggattaaaca aatcgcagaa gaaaaaggat tgtctattaa tgacgacaat
    21361 ttaaggaaag tgaagtaaat gagtcttaaa aataaaattt ggcggtttat ggaaaagaaa
    21421 aatctaaatc cgatgaacaa tcgcaaaaaa gtaggtatca ttttatttgc tacgagtatt
    21481 ggattgttct ttttatttgc cgtccgtttc tcgtatatcg ttattggagg tcatgttgct
    21541 ggtacctcat tagctgaaaa aacaaaacag ttgtaccaag gaagtgaagt agtaaaagca
    21601 aaaagaggaa cgatttatga tcgtaacggt gtggcgctcg cagaagatgc ctcctcttat
    21661 tcaattaaag cgatactctc aaaaacatat acctctgggg ataaaaaact gtatgttgaa
    21721 gaaaagaatt ttgataaaat agcagaaatc ctgcataaga atctttctat agacaaaaaa
    21781 gatgcactga atattttaga ggacggcgca aaaaaagagc tgtatcaagt tgaatttggt
    21841 agctatggga aaaatatctc ccaagaaacc aagcaaaaca tcgaagcgga catgaaaaaa
    21901 gaaggtgtcg ctggtcttta ttttgtcgac catcaggctc ggatgtatcc aaatggcgtc
    21961 ttttcttctc attttattgg atacgcagtt ccagacaaag atgagaatgg cttagttggt
    22021 aaactaggtt tggagtctgc ttacaatgat atcttgagcg gtaaagatgg taagattatt
    22081 tatcaaaaag ataattttca aaatcctctg ccaggtacag tggcagaaga agaaaaagct
    22141 gtcgacggac aagatatcta cacgacttta gacagcagac tacaaagtta tttagaaaca
    22201 ctgatggatc aagtaaatga agaatatcag ccagaagaat tgacagcagt cctgatgaaa
    22261 gcaaaaaccg gagaaatctt ggcaatggga caacgaccta ctttcaatcc tgaaacaatg
    22321 gaaggattga ctggaaaaga tgctatctgg cgtaatttct tggtacaaga tagttatgaa
    22381 ccaggatcaa ccatgaaagt tttcactact gcagctgcta tcgaagaagg agaattcaac
    22441 gagaatgaaa ccttccaatc tggaaaaatc caagtagaag atgctacgat caatgaccat
    22501 gactttggtg aaaaaggtgt gctgaccatg cgtcaagctc tttcatggtc aagtaacgtc
    22561 gggatggtca tactggaaca gcgattaggc ggacgatggt ataactattt acaaaaactg
    22621 ggatttggac aaagtactca ttctggatta gatgatgaag taaacggtgc tttacctaca
    22681 ttaaatattg tcgaccgagc aatgagtgct tatggacaag cggtaggtgt gacgaatttc
    22741 caaatgatga aagctttcac ttctattgcg aataacggta caatgattca acctcgatat
    22801 atcagcaaag tggtggatcc ccaaactgga gaagagcgaa caactcaaac agaagttcta
    22861 gggcaacctt tttcaaaaga aacaacagaa aaagtccgtg aatatatgag agatgttgtc
    22921 gaaagcgaaa actacggtag tgcttatggt gtatatagtg tacctggata caatgtatcg
    22981 gcaaaaaccg gaacagccca aatcgcttct gatacagggg gatatcaaac aggagataca
    23041 gcctacctgt attcgattgt ggagatggtt ccttctgaag atccagacta tgtcttgtat
    23101 ctaacaatga aacaccctaa gacatatgat cgaatggcat tggctaaaat cgcaaatcca
    23161 ttgatgaaac gggcaatgga ttttaaagaa actgaagaag attcagatac agaaacaaaa
    23221 acagaaaaag tatccgtagc cgattatcgt aatcttgaag cggacgttgc agcagctgat
    23281 gctcaaaaaa gtggactgca acctgtagtg atcggtaatg gtaaaaaagt acaaaaacaa
    23341 tctactgcca acggtgatca gttgatctct ggagaaaaac tgatcctata tactggtggt
    23401 gataagctga tgcctgacgt aacaggatgg tcaaaagcag atatcatgaa gctggggaaa
    23461 atcctcggta ttgaagttag ttttgatgga gatggttatt gtgtcaaaca agaacttgct
    23521 ccatatgaaa aaatcactga agacaaactt agttttactt tagaagaata agaagagaaa
    23581 taggagagaa aagagcatgg aatggacaca agcattgatt cccattgtca gcagttgtgc
    23641 aatgacaatt gccgcaatgc cgctatttat tggttatttt caaatgaaaa agcaaggtca
    23701 ggcgattcgt gaggagggac caaagtggca caatagcaaa gccggaactc ctacaatggg
    23761 cggcttagtg tttctgatcg gctctatttt gacaggaatt tgggttggag cttggcaaaa
    23821 acagctaact cctactcttt tcatcttgct ttttgtatta gcgttatacg gagtaatcgg
    23881 ctttcttgat gattttataa aaatattcaa gaaaagaaac atgggactga actcgaagca
    23941 gaaattgatt ggacagattc tagggggaat catcttttat cttgtttacc gttctgaagg
    24001 ttatccaggt gtattgaatt tcttcggact agatctacct ttaggaatca tttatggtat
    24061 tttcgctctc ttctggcttg ttggattttc aaatgcagta aatctgacag atggcatcga
    24121 tggattagtt gctggcttag gaagtatctc ttttgcagct tacggtctaa ttgcttggca
    24181 tcagcatcag tatgatgtat taatcatttg cttaagtgtt ttaggaggtt tactgggttt
    24241 cttcgtctac aacagaaaac ctgccaagat ttttatggga gatgtcggtt cattagcgtt
    24301 gggcggactt ttagcggcaa tttctataat gctaaaccaa gaatggacat tgcttttgat
    24361 tggtttgatc tatgttatgg aaacagcaag tgtgatgctt caagttactt cctttaagct
    24421 tacaggaaaa cgaattttta aaatgtctcc aattcatcat cactttgaaa tgtgcggatg
    24481 gtcggagtgg aagattgata cagttttttg gttgatcagc attgtgactt cgttgatcac
    24541 attatggatc gtttggtagg agatgacggg aaatgaaaaa aatatcaaca tatgaaaata
    24601 agaaagtgct tgtcttagga ctcgcaaaaa gcggagtaag tgcggccaaa ttattacatg
    24661 aattgggcgc acttgtcacc gttaatgatg gaaaaccttt tgacgaaaat ccggaagcac
    24721 aagagctgct ttctttaggg ataaaagtaa taacaggtag tcatccaatc gaattgttgg
    24781 atgaagaatt ttcattgatg gtcaaaaatc cagggattcc ctacagtcat cctttagtag
    24841 caaaagccca agaaatgggt attccggtaa ttacggaagt tgagctagct tacgaagtag
    24901 cggaatgccc aattattggg attacaggaa cgaacggaaa aacaacgaca acaacgatga
    24961 caggactact actgaatgca ggagcagatc aaggaattgc acgtttagcg ggtaatattg
    25021 ggtatccagc aagtggtgta gcgcaagaag caaaatcaga agataaaatt gtgatggaat
    25081 tgtctagctt ccaactcatg ggaatcacag atttccgccc tcatatagcg gtgattacca
    25141 atatctatga agcacatatt gattatcatg gtacaagaaa agaatatgtc aaagctaaat
    25201 ggaaccttca aaaaaacatg acagaaaaag attatctgat ccttaactgg aatcagtcag
    25261 aactacaaga attagcgcaa agaacaaaag cacgcgtact gcctttctcg acaaaagaag
    25321 tgttggaaga cggtgtgtat gctgacgatt actcaatata ctataaaaaa gaaaaaataa
    25381 tggaaatatc tgaacttggc gtaccaggta agcataatgt agaaaatgca ctagctgcta
    25441 tttctgtagc aaaattgtat ggtatatcaa atgaagctat acgagaaacg ttgcattttt
    25501 tccatggcgt accacatcgt acgcaatatg taggagaaat ccaaggcaga aaattttata
    25561 atgattcaaa agcaaccaat attttagcga ctaaaatggc tctaagtgga tttgaaacgt
    25621 ctaaggtcgt gttattggct ggcggattag acagaggaaa tacatttgat gaattaattc
    25681 cttcattaaa aggaatcaaa gcaatggtcg tatttggcca aacaaaagaa aagttgatgg
    25741 atgctggaaa aaaagcaggc atcgagacaa tagtaacagc cgattctgta gaacaagcag
    25801 ttcctttagc attagaaaac agtacagatg gtgatgtagt tttattatca ccagcgaatg
    25861 ccagctggga tcaataccca aattttgaaa ctagaggaaa cagattcatg gaagctgtga
    25921 accgattgta gaagcgagcg ataaaaaatg aaaattttgg ttacaggcgg aggaacaggg
    25981 ggacatattt atccagctct tgcctttgtc aattatgtaa agactcaaga accgaatgct
    26041 gaattcatgt atgtgggagc aaaacgaggt ctagaaaaca aaatcgtccc tgatacagga
    26101 atgcctttcc ataccttgga gattcaagga tttaaacgaa aaatttccat gcataacatc
    26161 aagacggtcc gattgtttct taagagtatc cgtcaagcaa aaaaaatcct cagtgatttt
    26221 caaccagata tcgttatcgg tactggagga tatgtttccg gagcggtagt gtacgctgca
    26281 gccaaaatgg gtattccaac agttatccat gaacaaaaca gtgtaccagg tgttaccaat
    26341 aaatttttaa gccgatatgt ggatcggatc gctctttcat ttaaagatgc ggcttccttt
    26401 tttcctgaaa acaaagcagt tcttgtaggt aatcctcgag cacaagaagt agcagatact
    26461 aaaaaatcag atattttgaa aacatttgat ttggatccag aaaagaaaac agtcttgatt
    26521 tttggcggta gtcaaggagc acttaaaatc aatcaagcag tatcagtttt tttaagtgaa
    26581 tatattcctt cttcttacca agttttgtat gcttcaggag atagatatta tgaagagatc
    26641 ctaaataaaa taaatcctga aatacaaaat attagtgtcc agccttatat caaaaatatg
    26701 gcagaggtca tggcttccag tgatctgctg gtggggcgtg ctggtgcaac ttcgatcgca
    26761 gaattcacag ctctggggtt accggcagtg ttggttccca gcccatatgt tacgaatgat
    26821 catcagacta aaaatgcaat gagtcttgtt cacgcaggag cagcaaaaat gattgcagat
    26881 aatgaactaa caggtgaatc actaagtcag acagtcaacg aaatcatggg tgatgaagaa
    26941 ttgcaaaagc aaatgtgccg agcttctaaa gaacaaggaa tccctgatgc ttcaaaacgt
    27001 ctttatgact tagtaaaaca aataatcaaa aaataaaggc ggtggtctta gtgagtaaga
    27061 aaaaagaacc aaaaaaattg tctcatccgt cttctgatga aaacaatttg actccttggc
    27121 aaaaggctaa ccgaaaatat ctggcagaac aagagatgaa aaaccaagag ccaaaaaaag
    27181 aagacataac attagataca cccctttcag attctgaaga agaaaaaacg gttcaagagg
    27241 aacagcaaga agaaagccag tccgagaaaa aaatcgaatt ttttgaagag atcaagcaaa
    27301 agaaaaacgg tggaccatat aacggttctt ttttgaaccg gctgcccaat ttaaaaagtc
    27361 aacggcacaa agtactattt cgtcggttag ctttgatcat cactgtattg ggtataccgc
    27421 tgattttttt gatctattat gtttctcctt atagcaaatt acaagcagtt gcagtatcag
    27481 gcaataagac agtcaacaga caagaaatca tctctgatac aaaattgtca ttaggagaaa
    27541 atgtttgggg acagtatttt catcgatcta cttatatcga tcgccttaaa aaagcacagc
    27601 caagaatcga aacagcaaat atacatttca aagggatgaa tgaattcgat ttggatgtaa
    27661 cagaatataa agagattgct ttgatcgcaa aaaacaatca atactaccca gtgattgaaa
    27721 atggaacagt attagatgaa aaagtagcaa atcctacaaa aaatctccct atcttggaag
    27781 agttcaaaga ctctgcaaaa atcaaagaat taacaaagca atacaatcag ttatcatcag
    27841 aattacaaaa agcaatttct gaaatcaagt acacgccaag agcaagcaat aaaaatctga
    27901 tccaattgaa tatgaatgac ggcaatcaag ttattgtaaa tatcaataat cttgcgaacc
    27961 aaatgaaata ttattcgcaa gtagccaaag atatggatga aaaaggtgtg atcgacatgg
    28021 aagttggtat cttctcgcat ccttacggta gcactcaaga aacggaagaa tcgggccaat
    28081 cggaagaaac aactgaaagt gaagaaaatg cggatcaaac aaacgaatca agtgaagagg
    28141 cagaaacgca aaaagcacaa acacctggat cgtctagcac aaacggatca tctgattcaa
    28201 atgattcaag tgaaacaagt caaatagaag ataatccaag tacaactccg tcaagtttaa
    28261 atgaaaattg ataaaacaac tttaaaaagt aagaatgtac tttctaaaaa ccagtaattt
    28321 atggtagaat tggaagcagt gaatatatct attaataagt atattttaaa acggacgcta
    28381 gttgttcgct tgtatatgga atagtaagta ggagggaatc ccattcatgg caaaaacagg
    28441 aatgtatgta ggccttgata ttggaacgac atctgttaaa gttgtcgtgg ctgaatatat
    28501 cgacagccaa atgaatatta ttggagtagg aaacgcaaaa tcggaaggga ttaaccgagg
    28561 cattatcgtt gatatcgata aaacggtcca agcgatacaa cgagcagtac gacaagcaga
    28621 agaaaaagca ggtatccaga tcaaaggcgt aagtgtcggt ttgccagcaa acatgcttga
    28681 agtagaaaat tgccagggaa tgattgcagt taacggagat tcaaaggaaa tcaccgatga
    28741 agatgtcaga aatgtggctt cagctgccct tgttcgttca atacctcctg aacgtcaaat
    28801 cgtgtcaata ttgccacaag attttactgt cgatgggttc gagggaatca aagatcctcg
    28861 tggaatgatc ggcgtaagat tagaaatgta cggtctgttg tttacaggac ctaaaacgat
    28921 catccataac atacgtaaat gcgtggaaaa tgccggatta atcgtcaacg aaatggtgat
    28981 tactccttta gcgctgactg aatcaattct ttctgacgga gaaaaagatt ttggtacgat
    29041 cgtcattgat atgggtggcg gccaaacaac aacggctgtt atgcatgata agcaattgaa
    29101 gtttaccaat cttgaccaag aaggtggcga atttgtcact aaagatatct ctattgtatt
    29161 aaatacgtca tttaacaatg cagaagcgtt gaaaataaac tacggggatg catacccaga
    29221 acggacatca ccagatgaag aatttccagt agatgtgatt ggtcagtcag aaccagtgaa
    29281 agtcgatgaa cgctacttgt cagaaattat ttcagctcga atggaacaga ttttcaacaa
    29341 agcaaaagat gcattggacc aaatcgaagc attagagttg ccaggtggcg tcgtattgac
    29401 aggcggagct gctagtcttc ctggtgttgt ggacttggca caagaaatct tcggcgtaaa
    29461 tgtaaaattg tatgttccaa atcacatggg gctgagaaac ccggtattta caaatgtaat
    29521 aagtatcgtg gattattcag caaacttgag tgaagtgtat caattggcaa aaattgctgt
    29581 tacgggtgaa acctcagcag ctcgtcaaat ggtagttgaa caagaaacaa caaacacata
    29641 tgagagctat gaagcacctg aagaaacaat ctacgatgag ccggaaccaa aagagtccgg
    29701 agaaaatgta aaaaataaaa tcaagggttt ctttacaaat atttttgatt aattcatcct
    29761 gggaggaata agatatggaa ttctcaattg ataataatat caatgacggc gcagtaatca
    29821 aagtcatcgg agtcggtggt ggcggtggaa atgctgtaaa ccgaatgatt gaagaaaacg
    29881 ttaaaggtgt tgaattcatt acagccaaca cagacgtgca agcattaaaa aattcaaaag
    29941 ctgaaacagt tatccaatta ggccccaaat atactcgtgg tttaggtgct ggttctcagc
    30001 cagaagttgg acaaaaagcg gcagaagaaa gtgaacaatc cttacgcgaa gctttagacg
    30061 gtgcagatat gattttcatc actgcaggaa tgggtggagg aaccggtaca ggtgctgcac
    30121 caatcgttgc aggtatcgcg aaagaattgg gcgcattgac tgttggtgtc gtgacacgtc
    30181 cattcacttt tgaaggaccg aaacgtggcc gttttgctgc agaaggtatc gctcgtttga
    30241 aagaaaatgt ggatacatta ttaattatct caaataaccg tttattagaa gttgtagata
    30301 aaaagacacc gatgcttgaa gctttccgtg aagcagataa tgttttacga caaggtgtac
    30361 aaggtatctc tgatttgatc acagcgccag gttacgttaa ccttgacttt gctgacgtga
    30421 aaacagtgat ggaaaaccaa ggtacagcat tgatggggat cggtgtggct agtggagaag
    30481 atcgcgtagt agaagcaacg aaaaaagcaa tctcctctcc attgcttgaa acatctattg
    30541 atggagctga acaagtactt cttaacatca caggcggttt ggatatgaca ttgttcgaag
    30601 cacaagatgc ttcagatatc gtagcaaatg cagcaactgg cgatgtaaat atcattctag
    30661 gtacatctat caatgaagaa atgggcgatg aaatccgtgt gacggtcatc gcaacaggta
    30721 tcgatgaatc aaaaaaggaa cgcaagtcta gccgaccagc tagacaagca caaatgcaat
    30781 cacctgctca aaagactgta ttagatatgg atcaagctaa accaacttct tcagaagaag
    30841 aaaacagttt tggcgattgg gatatcagac gtgaacaaaa tgttcgtcct agagttgatg
    30901 attccaattt tgacaatatc gaaaaaaaag aatttgatac attcaaccgc gaagaaacaa
    30961 aatctcatgg cgatgatgaa ttaaatacgc caccgttttt ccgtcgtaaa aaataaagga
    31021 gaggaagagc acatgattgc tgacaacttg cagaaaatca agcaagaaat tcaggattcg
    31081 tgtgctcttg ttcatcgtga tccgaaagaa gttacattag tagctgtaac aaaatctgtt
    31141 gaatccgctc aagctgaaga attggttaga gctggcgtta tgaattgtgc agaaaaccga
    31201 gtagataaat tattagaaaa gaaacaagat ttatttgatt ttcctgaaat aaagtggcat
    31261 ttaattggta atttacagcg cagaaaggta aaattgattg taaatgaaat tgattacttt
    31321 catgcgttgg acagtttaag attagcagaa gaaatccaga agcggttaga gaaagaatta
    31381 tcctgtttta ttgaagtaaa tgtttctggt gaggaatcaa aacatggaat ttcaccagat
    31441 gagcttttac cgtttgtgga atctttagct gcctgcacaa aaatcaaggt tgttgggttg
    31501 atgactatgg cgcctaaaga agcttctgat gagcaaatcc ataaagtgtt tggtacgctg
    31561 aaagaattgc gtgatcttgt tcaagcaaaa ggttatgccc atgcaccatg tagtgaatta
    31621 agtatgggga tgagtcaaga ctttcaaatt gcgattgaag aaggtgcaac atttatccgt
    31681 gtaggaacag cgctattcaa agacgaataa aggaggaaca aaaatgccgc ttttaaacag
    31741 aggagcaatt gctaattttt tcggattagc tgatgaggaa gagtacgaat accaagatta
    31801 tcctgaaaca acaaaaaagg ctgtacagcc aactaaaaaa aatacaacac agaatcaatc
    31861 aactcattct gtatctgctg cgaatactca tgaaaaacaa aaaacggcga cttcacgtcc
    31921 tgtaacgcat gaaagtattc agccggctga aaagaaaaaa gtaattgaac tacatcaatc
    31981 acctacaaaa actgtagcga aagaaagaac agcttcaaga acctctacac aacctaaagc
    32041 aaatcttgct ggaaaaatca ctgtaatgga accaagagct tattctgaag ctatggcgat
    32101 tgctaaacga atcattgctg gagaagcaac cttagtgaat tttcatttag tagaagaaaa
    32161 acaagcgaga agaatcgttg attttctaac aggaacagtt tatgcactag atggagatat
    32221 caaacgtgtt ggagatgaga tttttctttg tacaccgact gggatggaga ttgacagttc
    32281 aaccgctcaa tcttttgcag atgcgaattt gtttgatctg taagaggagg aactacgatt
    32341 tatactattg cactattact tcatttactt caacgggcag cctatttcta tagcattatc
    32401 ttgatggtat atgcattgct ctcttggttt ccaggcgggt atcaatcagg gctaggacga
    32461 ttattagcta agatctgcga accgtatatc agtttgttcg atcgtctgcc tttgaggatt
    32521 ggacccttgg atttcacaat tttaggggca atcctgatct tgaacttagc cgtacaaggt
    32581 ttagcacaga tcgttatatg gataactgtc catttagttt attgattgaa gcaacaaaat
    32641 ctttattctc agaatagata ggaggggaaa attttggatg caaatgtata ccagcacttt
    32701 cgggcagatg aacgtacatt tatcgattcg gtaggcgatt ggattgaaca ggttcaagga
    32761 caatatgcac cctatctgac cgaatttctt gacccaagac aatcctatat tttagaaaca
    32821 ctgattcgac aaagcagtga tttgcgtttc atgttttatg gcggatatga acaggcagaa
    32881 agaaaaagat gtttgatttt ccccgattat tacgaacctg tagaggaaga ttttgaaaca
    32941 gcattgtatg aaatccatta tcctagcaaa tttgccagcc taagtcatgg gaaaatcctt
    33001 ggaactttag tgggaacggg gatcaaacgc tcttactttg gcgatatcat ctcggacggt
    33061 gatcgctggc aagtatttat tgcaaatgag gtggatcatt ttgttgctgc acaagtaaca
    33121 aagattggaa aggttgctgt acgcttggaa ccaatcaaat acacagaaat gattatgccg
    33181 aaagatagct ggacacaaga aagaggaaca gttagttcca tgcgactaga cagtgtgatc
    33241 tctgagattt ataatatttc cagacagcgg tccaagcagc tgatcgaagc tggtaaagta
    33301 aaagtgaact ggacagaaaa tacaagacct gattttgtac ttgaactatt agatattatt
    33361 tcagtcagag gttatggcag agtccagatc caagaacttg aggggaagac gaaaaaagaa
    33421 aagtacaggg tactgttcgg tgtattacgt aaataaaata aaaaggtggt ttactcatgg
    33481 ctttaactcc attagatatc caaaataaga cattcccgac aaaaatgcgg ggatataatc
    33541 aagacgaagt agatgatttc ttagatttag ttgttcgtga ctatgaggaa ttgactcaaa
    33601 gaaatcgaga attagaaaaa gcagtcaaac attctgaaga aaaacttgag tacttcaatg
    33661 aattaaaaga tgctttgaac caatcaatca tcgtagctca agatacggct gataaagtaa
    33721 aaacaagtgc aagcaaggaa tccgaagtga tcgttacttc tgcacaaaac aaagcagatg
    33781 agttagttgc gaacgctgaa aaacgtgcac atcaattaac aactgatgca gaagaaaaag
    33841 caagaaaaat cttgacagat gcaacagaaa aagcaagaca gcttgcgaca gaaactgaag
    33901 atcttaagaa aaaaacacga gtattccatc aacgtatcag cttgatgctt gagtctcaat
    33961 tagaacaagt gaaaagtccg gaatgggatg aaattttaca accattctcc agctacgtga
    34021 ctgacagcca tgaagtgatc aaagaagttt tagcaaaaca gcttgacaat gaaaatgata
    34081 ctgatgtaaa ctcagacaca ggagtcatag aagcacctat gacggcattt gatacacagg
    34141 ctattgatat ctcgatcgtg cctggtgaaa atgaaaacga gtagaacaga aaagcacttt
    34201 gttattctta aagcgagccg atgagagtgg aagttcggta agacactgca tggtgaaaga
    34261 tcctctactt gagacctgct tttccgaaat tagagtaaga agcagcggct gacctcgtta
    34321 ttggtcacag agagtttgca ctagatagat gcaactaaat ttgggtggta ccacggcatg
    34381 ttcgtccctt gcggctgaac atgctttttt gtgtccggaa aacaaataaa ggagtgtatg
    34441 gttgaatgaa aatgaaagaa accttgcaat taggaaaaac aaaattccct atgcgtggta
    34501 atttgccaaa ccgagaaggc gagtggcaaa aagaatggga agaaaatcga atctaccaaa
    34561 aaagacaaga gttgaacgaa ggaaaaccta gctttgttct tcatgatgga cctccatatg
    34621 ccaatggtaa tatccattta ggacattcat tgaataaaat cagtaaagac attatcattc
    34681 gttcaaaatc aatgtctggc tatcgtgcac catatgttcc aggatgggat acacatggat
    34741 tgccgattga acaagtattg gctaataaag gcgtgaaacg taaagaaatg tccatggcag
    34801 aatacttgga aaaatgtcgt gaatatgcac tttcccaagt agataagcaa cgagcagatt
    34861 tcaaacgcct aggagttgca ggagattggg aacatcctta tgttacatta gatccttctt
    34921 atgaagctgc acaagtacgc gtatttggta aaatggctga aaaaagctat atttacaaag
    34981 gattgaaacc aatttactgg tctccgtcaa gtgagtcttc tttagcggaa gctgaaattg
    35041 aatataaaga tgtaaaatct ccatctattt ttgtagcttt caatgtcaaa gacggcaaag
    35101 atattcttga tgaagataca gcttttgtca tttggacgac aacaccatgg acattgccag
    35161 caaaccaagg tattgctgta aatccaaaat ttacgtatgt attggtggaa gccgatggcc
    35221 gtaagtttgt cgtagcgaaa gatttgctag aaacagtcca atcagaaatc ggttgggaaa
    35281 atgtaacagt tctaaaagaa tttgctggac aagaaatgga atatatgact gcaagtcatc
    35341 cattctatga acgtgaatca cttgtgattt taggcgatca tgttacttta gacgctggta
    35401 ctggattagt ccatactgct ccaggacatg gggaagatga ctatatcgct ggtagcaaat
    35461 ataaactatc tgtagtgtca cctgtagact caaaaggatt gtttacagaa gaagcacctg
    35521 gatttgaagg tatcttttat gacaaagcaa atcctatgat cactgatcta ttaaaagaaa
    35581 aaggagcttt gctgaagcta gatttcttta ctcatagtta tcctcacgat tggcggacga
    35641 aaaagcctgt gatctatcgg gcaactccac aatggtttgc atctatcgat aaattccgtc
    35701 agaatatttt ggatgaagta gaaaaagtag agtgggttat cccatggggt aaaacacgtt
    35761 tgtataatat gatccgtgac cgcggcgatt gggtaatttc ccgtcagcgt gcatggggag
    35821 taccacttcc gattttctat gctgaaaatg gagaagcaat catcacacca gaaacaatcg
    35881 aacatgtggc aaaattattt gctgaacatg gatcaattat ctggtttgaa aaagaagcga
    35941 aagaacttct tcctgaagga tttacccacc caggttctcc taatggcgaa tttacaaaag
    36001 aaaaagatat catggatgtc tggtttgatt ctggttcttc tcatgaagct gttttacgtc
    36061 agcgaccaga attatcattc ccagcagata tgtatttaga aggatcagac caatatcgtg
    36121 gctggttcaa ctcaagtatc acgactagtg tggctatcaa tggtgtagca ccatacaaat
    36181 cagttatttc tcaaggattt gctttagatg gtgaaggccg taagatgagt aaatctttag
    36241 gaaacatcat tgcaccagaa aaagtgatca aacaatttgg tgcagatatc cttcgcttat
    36301 gggtatctag tgtagattat gaagcagatg ttcgtgtttc tatggatatc ttaagtcaag
    36361 tggcagaagt atatcgtaaa atcagaaata cgatgcgttt cttattagcg aatacagaag
    36421 atttcgatcc taaaaaagat actgtttcgt acaatgattt acgttctgta gacaaatata
    36481 tgaccgttcg attgaaccaa gtaatcaaag aaatccgaga agaagggtac gacaaatata
    36541 acttcatgca tatttaccgt acagttatga acttcttgac ggttgatcta tcgtcattct
    36601 acctggattt tgctaaagat gtagtttata ttgaagcaga agatgattat caacgtcgtt
    36661 gcatgcaaac tgttttctat caaacagctg tagctttgac aaaattattg actcctatca
    36721 ttccgcatac agcagaagaa atctggtcat tcctgaaaga agaagaggag tacgttcagt
    36781 taagcgagtt tcctggttat gaagagtttg caaaccaaga agaattgatg gatacctgga
    36841 ctgctttcat ggacttccgt gataatgtct taaaagcatt ggaagaagca agaaacagca
    36901 aattgattgg taaatctttg gaagcaaaac taactgttta tccaaaacaa caagttcgtg
    36961 aaatgttaaa agctttggat gcagatattg cacaattatt gatcgtttca ccagactact
    37021 ttgaagttat ggcagcagat gaacaagtgc cagataacgc tatggtattt gatgatgttg
    37081 ctattacagt ggaaaaagca gacggggaaa cttgcgatcg ctgtcgccaa gttcgtaaag
    37141 atgtaggagt agatgaaaaa cttcctcatc tatgcggtcg ttgtgcaaaa atcgttgaag
    37201 acttctatcc tgaagctgta gctgaaggat tcgaagaaaa gtaaaaggga aaagggggtg
    37261 tgacatgttc tttgtcacac ccccttttgt tatcaatcgt ttaattttcc acgttcacga
    37321 taccagttat ctggttgcca gatccattcg aatccgtctt tttcaagtag ttcgaaagct
    37381 gctttaggtc ccattgatcc agctttgtag ttgggaaaat ccggttgtgt ttggtcccaa
    37441 gcttttcgaa tgacatccac gatatgccaa gattgagcga cttcttccca atgagagaag
    37501 ttcgttccat caccattcaa cgcatctaat agtaattttt cgtaagcttc tgggctattt
    37561 tcaaccattt ctgcactatt tctaaattcc aatttgattg gttccgtttc aaatccttgc
    37621 cctacttctt tcccattcaa ggataaagaa aatccttcag ttggctgaat atagatcgtt
    37681 agaacattag gtggaagtgt cgtatcgtca catggttcat cgacagatgt tttgaataca
    37741 ttaacaggaa cttgtttgaa tacgatgttg atccgagtcc ctttttcagt caaacgtttg
    37801 cctgtacgta catagaaagg aacacctgac caacggaagt tatcgatagt gaatttgcct
    37861 gcgacgaatg tttcagtctc tgattgagca tccactttgt cttcttcgcg atagcccttg
    37921 aattgtacac catccaattc accttgagca tattgcccgc gaacgaagtt ttctaacact
    37981 tcttcttcgt tataaatgcg gatagctttc aatgctttga tcttttctgt acggatttct
    38041 ttttcggaaa atgctactgg cggttccatt gcaagtaatg ctacaacctg taaaatatga
    38101 ttttgcacca tgtctttaag tgcgccacta tgatcgtagt agcccccgcg atcttctaca
    38161 ccgaggtttt cgccaaaagt aatttggata ttgtcgatat agcgattgtt ccattgagcc
    38221 tcaaaaatat tattggcaaa acggatagct gagatgtttt ggatcatttc ttttcctaaa
    38281 taatgatcaa tcctgaaaat atcttgttct gggaatactt ctctgatttg ctggttcaat
    38341 tcaaaagcag actggtaatc agaaccgaac ggtttttcta tgatcaaacg attgaagcca
    38401 ttttttgtca taatattttg tgatttcaaa tgagaaacga tcgttccaaa aaattgaggt
    38461 gccatagcca aataatagac acggttaccg tttaattgat agtgtccatc taatcggtca
    38521 gacaattctt ttaatgtctg ataatgcgtt gaatcattta catcatggga ttgatagtaa
    38581 aaatggcttg caaaggtagt tgcttcttca tctgaaggat tcaatgcacg gattgtttct
    38641 ttgacaactt cacggtagtg ctcatcgctc cagggacggc gagcggttcc gattacagca
    38701 aaatgctcgc ctaaatttcc ttttctgtaa aggcggaaca aagacgggta caatttacgc
    38761 tgggctaggt cacctgtacc gccgaatatc gtaaacaata catttttttc attagacatg
    38821 tggatacact cctttatatt ttatcgacaa aaatcgtgct ggccgcctta aagctgacgg
    38881 ggattttctt tctatcattt tggatagtga tcggtccttc ataagctgcg atgtcaacga
    38941 tctcatattc ttcgttgata ttcaggtcaa tagtgactaa gtaatccaac aattcttttt
    39001 catcaagcac tcgtgcgata cgcacttttg taccgacagg ataggactcc aatgtttgtc
    39061 ggcgttcttc atggacaact tcctcttttt taggaatcga tcctccatga ggacaatgtt
    39121 caggatgatt caaatagttt tctaaatgat ctgcaagatg ttccgaagtc acatgttcca
    39181 atacttcagc atcatcatgg acttcattcc agctatagtc taaatgttca actaaaaaaa
    39241 cttcccataa gcgatgtttg cgaatcagac tgctagcttt ttgcagtccg cttgcagtca
    39301 attgtacacc ttgataaggg gaatgttcta caagctcttc tttcacaagt ttagaaatca
    39361 tttcactgac agatgcagct gagacatcta gactagatac gatttgttta ttgttgactt
    39421 tcgttgtatc tccacctagt tctaaaatga ttttcaaata atcttctcga tttggtgtca
    39481 tattcgatca tcctttcatg gtaaaaaacc atcattagta agtgtaacaa aaaaaacgtt
    39541 aattgactat ttttctaaat aaaaaacaaa ggatttttag acgtttttac aacaaaaaac
    39601 gaaagatgat agagaagact cattctttcg tttataaagg atatatatat acattgggag
    39661 ggatctattt ttcagataac aacttatatg aagtgtctgc agattcgtca taaagagttt
    39721 gttcgggata agaaaagata agataaaaac catactgatc acgtacgact aaacgatatt
    39781 catctatttc taccacagat gtgccaagag actgaccgtt gatcaaaaac gtgtaatcag
    39841 atgtaatctg taattgttgt ttttgctggc tatcactaaa gaaccatgta ccttgatact
    39901 ttgagagtcc ttctgacttt tccgcttgtt ttttcttttt caagtaaata aaacttgaga
    39961 gaacggtaat gagtgaacca agaaaataga tcattttttt tatcaccaaa tcaccccttt
    40021 atgagaaaat aatcgcatag acatattctt tcttctttat ttcattgtac cgaataaaag
    40081 ctaaaatttc tctaagactt tcgactggtt tttttactgt ttttctacaa attatgatag
    40141 gatacatagt aggaagaaag gtgacataat ttgaagtatc agcaagtgga aatagctttt
    40201 tttattgacc gcccgaatcg gtttatagca cattgtatga aaaaagacgg agaaataatt
    40261 attgcgcatg tcaaaaatac aggtagagga aaagaggtcc tgataccagg cgctgaagtg
    40321 gctattgtat ttgctcctgg acctaaacgg aaaaccgcct atgatctgat tgctgtaaaa
    40381 aagaagggtg actggtttaa tatcgatagt caattgccta atcggctggc aattgatggt
    40441 attttagacg gcacgatcct tttaccagga ttaactggtc catttacggt atacaagcgt
    40501 gaagtaactt atcttcactc caaatttgat atttatttag agacggtggt cggacaaaaa
    40561 gcatttgttg aagtcaaagg tatgacactt gaaaataaag cgattggtgc ttttcctgat
    40621 gcgcccacac tcagaggatt gaaacatata ggagaattaa cgtatgccgc acaagatggt
    40681 tatgccgctt atgtcttgtt tattgctcag ttcgaacatc ttcatctggc aacgatccat
    40741 gaagaaatgc aaccagcact agctgatatg gttcgacatg cgcagcaaag cggagttcaa
    40801 atcctcgcct ataattgtca agtatcggca gatgaggtaa ctgtcaagca ggccattcca
    40861 tttgatgtag atatcccttt tgaagatcct atacaataga aagaaggtaa aataatgatc
    40921 aacttaggta ttatcggaac aaattggatt actcatcaat ttgtccaagc agctttaagc
    40981 acaaaaaaat atgaattgac agcggtatat tctagacatt tggaaaaagc acaggaattt
    41041 ggttctcatt atgaagggga tatcgaattc gcaacagatt tgaagacttt tttcaatatt
    41101 actcatatgg acaccattta tattgcttct ccgaatagtc ttcactttag tcaagctaag
    41161 caagcaattt tggcaggaaa aaatgtgata gttgaaaagc cagctttttc gacgccaaaa
    41221 gaaatggatg aaatcatcca tttggcaaat gaacacaatg tgtatttttt tgaagcagca
    41281 agaaatatcc atgaatcagg ttttaaaaag attgccgatt acttaccatt aaaagacgag
    41341 ataatcggtg caaatttcag ctatatgaaa tattcttcgc gctatgacca agtattagat
    41401 ggagaagaac cgaatatctt ctcgactcat ttctcaggtg gtgcattgat ggatttaggt
    41461 gtttaccttg tatatgcagc tgttgcttgg tttggaatgc caaaagaagt ccattatttc
    41521 ccaagaaaga tttctactgg tgtagatggt cttggatggg ggatcttacg ttatgagaat
    41581 tttgatgtat cgatccagcc aggtaaaatc ggtgattctt atttaccgtc agagatttat
    41641 tttgactcag gaacgctagt gatgaatgga gtcaatgcaa ttgaacaagc acaatttcat
    41701 gatcgcgcgc ataaagaaat cattgatttg gcggtaacag ctgcagaaaa tccaatggca
    41761 gatgaagcag aggattttgc caatgtcatg gctcatccga aagatccagc ttggggattg
    41821 ttgtatgaag agtggatcga gctagctaga aatgtgaacc aagtgattta tgatttaaga
    41881 aaaaatggcg gcatcaaatt cgatgcagat aatgaggaag atttttaatg aacgaatttg
    41941 tacaagtatt tcccgaaaat tggcagaaaa aatatgagga taaagggttt atctcacctt
    42001 ctcttattca acgagcagtt tttcagccac taagtaataa acaaagtata gtagcggttt
    42061 ctcctacagg ttcaggtaaa acactggctt atctttggcc tctactacta aacgtggaac
    42121 caggtgaagc aagtgcgttg gtcatttttg cttcttctca agaactagct attcaagtag
    42181 cagatgttgc acgtgaatgg ggaaaagaca aagagttgaa agtccaatct ttggtgggcg
    42241 gagccaacgt caaacgtcag attgagggtt tgaaggaaaa accagaaatc ttgattggta
    42301 caccaggaag aatattggaa ttgatgaaag caaaaaaaat aaaagcacat caagtgaaaa
    42361 caatggtatt tgatgaagca gaccagctat ttgacgctgg gaacagccaa atcattgatc
    42421 aaattcttca tcaagctcca acagagtatc agctagcttt tttttcagcg acagcagatc
    42481 gttcccttga aagcattgaa aaaatcactg ggaaaacgtt tgagacaatt gacgtgacag
    42541 cagaggacga ttctcgaaaa ggactgagac attacttttt acgtgtaccg atacgaaaga
    42601 aagatgacta cttgagacgt ctcacgcata tagaagattt tcatggtctg attttcttta
    42661 atcaactgac tgaattaggc gtaatggaag aaaaattgct gtatagaggt gtaccggtag
    42721 gaagtctggc ctctgatcag aacaagctac tccgaaaagc agcgctagaa caattcaaaa
    42781 agaaaatggt cagagcactt ttgaccacag atgtggcggc tagaggttta gatattacag
    42841 gtcttcctta tgtagtgaat gcagaagttc cgttaagtga agaagcttat ttgcatcgtt
    42901 cgggaagaac tggtcgaatg ggtaatgaag gaatagtaat tacactcgta caagataata
    42961 atctaagaga tttaaaacgg cttttacgtc catcaaatat aacacttgaa gagatttttt
    43021 tatacggagg taagctgcaa actgagcagc caaccaaaga agatgcagct cctgcagtaa
    43081 ataaaaagta tgcgaaaact ttttctggaa aggagcagaa cccagaatat aacaaagaaa
    43141 aaataagaaa aaaagttaaa aataaatcaa aaaaagagag aaataaagga gcaaggcgca
    43201 aataagcgcc tttttatgct atgattatga aaacgggaac aaaattggtt gccagaggag
    43261 gggttatgtg aaagaacgtc aagaagagat cttgcagcta ttgacagaaa accaacaagg
    43321 attgacagca agtgatgtag ctgagcgctt aactatcgat cgaagcaatg caagccgata
    43381 tttaagtgaa ctatacaagg cccatcatat tgtaaaaacc gctggccgac cagtcgttta
    43441 ctctttgcca acggaaaagt caaaatctga tgaagtgcat gtcgactctt ctacgcaagt
    43501 gacttttgaa acactcgttg gagaaaatga ttctttaaaa gtcagcatac agcaggcaaa
    43561 agctgcgatt ctttatccac cgcgggggtt gcatacgatt atttttggag aaactggtac
    43621 agggaagtcg atgtttgctg aatgtatgta tcattttgca atcgattcag agatgctttc
    43681 tgcagatgcg ccatttgttt ctttcaattg tgcggattat gctcaaaatc cgcaactgct
    43741 ctttggacac atctttggga tcaaaaaagg ggcttacaca ggagcagctc aagatagtcc
    43801 cggtctaata gccaaagcag acggcggtat attgttttta gatgagatcc accgtttacc
    43861 gccggaaggg caggaaatgc tgtttacttt cattgataaa ggtatttacc gtccgcttgg
    43921 agaaagtgca caagtacatg aagcatctgt tcagatcatt ggggcgacga ctgaatcttc
    43981 agaaagtttc ttatctacgt tcaatcgacg gattcctatg gcaatcacct tgccgaatct
    44041 tgcttcacgt tcactggatg agcgttatga aatcatctct ctttttatca aacaagaagc
    44101 gaatcgtttg aatcaacgta tcgatatcga aaaagaagcg attcttgcct tcatgcttta
    44161 tgatgcggaa ggaaatatcg gtcaagtgaa acgagacttg aaactagtct gtgcaaaatc
    44221 ttttttacat taccgtactc atcatgagaa aaagctaatc attcgaaaag aagaactttc
    44281 ccttcaagta caaaaaggat tactgaaaat caaggaaata ccggaacgtc tcgaccgatt
    44341 catggacagc aaaagtcagt atttgacatt tgaaccagga tttgctgatg ttgtctggtc
    44401 acaagatcca gaacgaaaca tgcaggtcta taacgatatt gaggaaaaag tcacttctct
    44461 ttccgaaacc ggtgtggaga acattgatct agagaccctc atttcaaaag atgtggatgc
    44521 ttatttccag acatatgtca aagaattgac taaaagttcg attcccaaag atcttttgcc
    44581 agatgaaatc tggcggttaa ccaatcaatt gtatgatgtg gctgaaaaac ggttagaccg
    44641 tatttataat gaaaaagctc gttttgcttt tgctttgcat ctgcaaagta cattggatcg
    44701 tgtgagagaa ggtcatatga tcgtccaccc agatttgaat aacgtacgga aaaattttaa
    44761 gagggaattc caggtagcaa tcgatctttc aacgatcatt gaagaagaag aacaagttga
    44821 gattcctttt gatgaaatcg gctttattag tatgtttctt tctatcaatc taggagaaag
    44881 tgagcctgtt aaagaaaaca aagtagatgt tctcatattg atgcacggca gacagactgc
    44941 ttcaagcatg ttggagatgg cacaagaact attaggaaca aaagtaggga aagcttttaa
    45001 tatgccactg gttatggaag ttcaaacaat gtatgaagaa gtgttacaat atgtcaaaaa
    45061 ctatcaagag catttgacga atggcctact cttgttaaca gatatgggat cattaaacac
    45121 cttcagtgat ttgatttatg aagagactgg cattcggacg aaagctatca cgatgacaag
    45181 taccatgatt gtgatagaag cgatacggat ggcaagtgtt ggaagaagct tagaagatat
    45241 ttatcaaaat atccagcttt cctttgaatc gatcgtacgt gatcagttca aagcggaaaa
    45301 gtctgaaact ttacagcgta aaaaagccgt aatcgtgaca tgctttactg gtgaaggagt
    45361 agctgccaaa ttatatcagc gtattatgcc agtgattaat caaaataagg tagaactgat
    45421 ccagatgcag tttttagaac gtgaatcatt caaaaaacac atcgatgcct tattggaaga
    45481 atatgagatc aaagcaattg cgggaacagt tgatgttgat tatcaaaata tcccattttt
    45541 ttcagcctat gacgtttttg atgatgaaaa actaaatatt ttaaagcgta tagctgatga
    45601 tgagatcccg acagctaaaa ttgtcgattc acttgctgga tcattaagcg atgtcttttc
    45661 tattgatgaa ctgatccgtt cattgcaaaa gacagttcag cagatccaga ccgatttgtc
    45721 gattatcgtg gaacctggag tagatactgg aatcgttatc catttagcct tcatgttaga
    45781 cggattgatc aaacaagaaa gccatcggca atttgagaaa ctgtctattt tctcaaaaat
    45841 ttatcgtttg gaaatggata tcgttcgggc aaacttgatg gcactagaac gaaaatatca
    45901 ggtaaaaatt ccagaagatg atatcgcatt tcttacacaa atgtttcttg aaaataaaat
    45961 aaaaaaatga acacactaat catactgttt acacactaaa catctgtgtg cgcactataa
    46021 aaaagagcta aaaagcatga aaacgctatt tcagctcttt tttttattgg catgcttctt
    46081 gcgttatata aaagtgtaaa aaaataaaag gagtgttaaa aatggcaaaa aaaacaatca
    46141 tgttagtttg ttcagcaggg atgagtacga gtttattggt cacaaaaatg caaaaagcag
    46201 cagaagaaaa agggatggaa gccgatattt ttgcggtctc tgcctcagat gcagataata
    46261 atttagaatc taagaatgtc gatgtattat tattaggtcc tcaagtacgt ttcatgaaag
    46321 ctcaatttga acaaaaactt gcgcctaaag gtattccatt agatgtcatc aatatgcaag
    46381 actatggcat gatgaacgga gaaaaagttc ttgcacaagc agaaaatcta atgaaataaa
    46441 tcaagtatgc agttgggggt ataacaacgt ggaagatcaa caaaatctag aagccatcat
    46501 ggggttaatc atgtttggcg gtaatgcaaa gagcgatgca atggaagcga ttgcggctgc
    46561 taaaatcggt gattttgagt tagcggatag gaaaatcgct gatgcagaag aatcacttat
    46621 acaagcgcat catgcacaaa cagacatgtt gacacaagaa gcacaaggaa atcatatgca
    46681 agtgacatta ttgactgttc acagtcaaga tcacttaatg acatctattg cgtttactga
    46741 tttagcaaaa gaaatcatcg aactttatcg tcgcgtatac agcgaataaa tacaacttct
    46801 tttttgaacg gaagaagaaa aatttttgaa cggatttttt attttgttat aaggagaact
    46861 ctctttatat aaaaaactta taggaggaag taaaaaatga acggactatc aagttggatg
    46921 gagaaataca tcctgccggt tgccgcaaaa atcggagcgc aaaaacattt agttgctttg
    46981 cgtgatgctt ttatcggcac actgcccgca acaatggcgg gatcagtcgc tgtaatgatc
    47041 aatgcaatta ttcgtgattt accgcaacag tttaacagta cctatgctga cagtttagca
    47101 gcagacaaag gaatctttgc cgtattgaat gtcatcatcg gaatcaatgg ttttgtctgg
    47161 aacggtacac tagcaatcgc cggattgatc tttgcctttt cttgggggta taatcttgcc
    47221 cgagcttatg gagtaaatga tctagctggg gggatcgttg gtttagctac cttgattcaa
    47281 ggtatttcct ttggtccagc attatcagca gcattagatg tgaatgttcc ttcaaatgtc
    47341 gcagatgcaa tcaatagttc tgagattggt gcaacgattg cagatggcac actgtcagtt
    47401 ggattatggg gttggctaaa actagatcat ttgaatggaa acgcttattt tacagttatg
    47461 atcatgggag ctatttcagt cattattttt tgtaaattga tgcttgccaa cattacgatc
    47521 aaaatgcctg attcagtacc accagcagta tctaaagcat ttgcagccat tttaccagcg
    47581 acgatcgctt tatacgtcat tgctattatc aattatatcg taggtcgtgt agctgatggg
    47641 caactgatca ttgatttggt gcaaaaatat attgcagaac cattcctagg attatctcaa
    47701 gggttgggag cagtattgat cgtcacagta tttgttcaag tattctggtt ctttggtatt
    47761 catggaccaa atgtattggc accagtgcta gagggaatct ggggacaagc acaactaatc
    47821 aatattgata ttttccaaaa aggttataac ggaaaaactg gtacaccagc cgtgttagat
    47881 gcaatcgaag atggaaaagc ttatatgtgg gtccgcggat cgttcgatgc ttttgcatgg
    47941 tttggcggat caggcggaac gatcgtctta ctgatagcta tcttgatttt ctctaaacgt
    48001 gcagattatc taacagtagc taaattatcc attgggcctg gtatcttcaa tatcaacgaa
    48061 ccagtgatgt ttggtctgcc aatcgtattg aatgcaatca tgtttatccc attcttgatt
    48121 gctccagtag tagcaacagc aattggatgg acagctactt atcttggcct agtcgctccg
    48181 gtatcgcagc aagtcacatg ggtagtaccg ccgttcttat tgtcattctt agcaacagga
    48241 gcggattggc gggcaccgat cgtaacatta gtatgtatgg tcgtgacttt cttgatttgg
    48301 gcaccatttg tcatctctgc aaacaaaatg gaccctgccg caggcgaaca acaataagat
    48361 gaacgtgagg agcgggacgc tttaagtttc gttcctcata atgaattttc gaggaggaac
    48421 tgaaatgatt acaattgcca gtgcaatgca acaagatgaa atcaaagaat tgttagaagg
    48481 atacaatgaa gaaggaatga cttttacatt taaagaaaaa caaggaatca agctgttctt
    48541 tgaaacaagt gcagaagatc cggaagttgc ggctaaaaaa gcaaaggaac taatcaaagc
    48601 acagccttgg ggaacagtcc tttattttca atcaacagct gtttagaaaa tggtttaaaa
    48661 agataggatg gttgagatga taaaaaacgg tttattttta tgtagtattg gttctttgat
    48721 cgtgttgtat agtttgaatc cacattcgca aaggtatgat ttgtatagta tggtcacagg
    48781 tctttttcta acaggattag gaatctattt cttcatgaag gggaaaaaaa gagaagaagc
    48841 aaaggagaaa aataactaat ggaaatcacg aaaaaattaa atattccagc atcgtttttt
    48901 tacgataaag ttatggactc tgtccttttc gatgtacgta aagcgaccgg caaatcagta
    48961 actagaaaac agctgaaaaa tttggagtac gtcaaacaat tctcgcaaaa taaccgggct
    49021 cgcataaaaa tcgaagaagt cattgaaaac cagtcttata agtttcgtac atcgacaacg
    49081 aagaatgaat tcgtcgttca ttatcaaatc aaaccattgg acgattcgac ctgtgaggtt
    49141 cattacacag aaaaaatgga gtcttatggc atgcttcaaa agttgaatga tatgctttta
    49201 ggaacaatct tgatgtattt taaacggaga cgtttcaaaa aaatgctaga tatgatcgaa
    49261 gcaacgtatt aaatcaatga aacatggtac tatttagtta ttgaaaaatg aagtaagcta
    49321 gaaggaattt ttcataggaa gatagatctt tcctgcgaat cctcactagc ttttttgtag
    49381 gaaggggcag caaaatgaca aacaaagtaa gagagctagg tgtatcttcg attgaggaaa
    49441 tgtttgattt agccacttat gcatttaatg gggcagatac acctgaaagg cgagaacgtt
    49501 ttcgtacact tgcggagaat tcttggaatt acggattttt tgatgaggag gatcggctaa
    49561 caagtcaagt catggcgacc ccttttccag tgaattttta cggacaagag tacctaatgg
    49621 ccggcattgg ctatgttgct tcttatccag aagcaagggg acaaggcggg atcaatcaga
    49681 tcatggaaaa gatccttgaa gattgccgtg atcgtaaagt aagcttatct tatctggcgc
    49741 cattctccta tcccttctat cggcgctatg gctatgaaca atcatttgat aaaatcagtt
    49801 atcaactaaa cagtcgtgat gtgccgttta tcaaaaaaaa atctggaaaa ataaagcgta
    49861 tggattttga agatgcaaaa gctgcaatca gaagaattta tgaacagatg ccggaaaacc
    49921 aacgagctgg tctgaagcgc gaagactggt ggtatacgta taaattcaag caaaaaggca
    49981 attaccagtt tgctttgcat tttgatgata atgaggaagc aacgggatat atcgtttatc
    50041 agcttgcaac gtcttccttt attattgcgg agtggggatt tttggatcat gatgcttttt
    50101 gcagccttgt tcgcttcgta agttctcaca atggtgcttt tgaaacattt gaatattcat
    50161 gcggctacgg cggtaatgat caaaattatc tattagaaaa tccatttgct tctgtaatga
    50221 tcacacctta tatgatggcg aggatcgtag atatttcttt gtttttggaa aattatccat
    50281 ttaaaaagag acaagctgca tttgccctac aaatagaaga agatcattat gcgaaatgga
    50341 atgaaggaat cgtggaagta acaatcgaaa atggaaaaaa tcatctctca aaagtagaac
    50401 aaacaaaact cccaatcgtt cgaggaagta tccaacatat cacgcagcta ttattagggt
    50461 atcgtaaagt ggaagagttg gtattccaag aaagaataca ggtgacaaaa gaatatgaag
    50521 aaacgttaag ctcaatcttg ccacagcaac agccaattct taatgactat ttttgataaa
    50581 aaacgggtga tgacaatatt tttgtcacca cccttttgtt tttcatttgg catttgaagg
    50641 aaaatgttaa agtaaatcct cttttttcgt aaatttgaat tttttgtttt cttttgtttt
    50701 agggttttct tttttgtggt cttggattgc ttctttcgta atcttttccc gatttttttt
    50761 agatgaatct aagcgttctg cgtatacttt ttcttttggc ttatcattca ttggaaagga
    50821 cctccctaca ttatttcatt ctacctctag tttatcatct gttaaggact agtggaaacg
    50881 ttttgcctaa cttataagct gattttttta gcagataagc ttgcaagtaa aaaacaaaca
    50941 agctacaatt gatttattga aattgttcct gtagctcagt aggatagagc aatcgcctcc
    51001 taagcgatag gtcgctggtt caattccagt caggaacgta taaataaccc ctgtatcctt
    51061 tgcggtacag gggtttgttt tgtatttgtg ccaaaaatgt gccaaactag ttatcatttt
    51121 tatataaact atcgaacaca gtattgattt tttctttatc ctgatcttca agctctcgaa
    51181 caatatgaag ataagtagac attgtagttt ctaaactgct atgtcctaaa cgtttcgata
    51241 cgctaagtat atttactcct tgataaagta aaatagatgc atgtgtatgc cttagtccat
    51301 gcaaagtaaa ttgcttttca atccctaatt cttttaattt tttgcgcaaa tatttgctga
    51361 cggcgtttga tgaaactaat ccatttttta agttgaaaaa aacaaaatta tgagggtttt
    51421 tgatttcaaa attctcgaat aattcttttt gattgatttt aaactttttt aagagatcaa
    51481 tcgtatgttt atcaatggaa attttcctat ttgaagtttc gttttttgtt tttccccatt
    51541 catttaattt ataattccat gttttattaa tagtgatgat ttgatcttcg aaatcgatat
    51601 cttcccatgt taatcctaga agttcagcaa atcgcattcc agtggcacca gcaactaaca
    51661 cgagcatagg agaagagtac tgcgcagaaa ggttttcttc tacgagtttc attaaagatt
    51721 taaattgatc ataatctaaa tatttatctt cttttggctt caatgaatct tttcctttga
    51781 ttactgcttt tctagttgga tcaaacggaa ttaatccttc ttctacggca tctttcaacg
    51841 acgctctaat atggttatta aacttaataa cggtagattt tacatggtct ttagcatact
    51901 tatttaaaaa gcgttgatat cccactctat ctaaatcaga aatcaatacc gctggcatat
    51961 atttctttat gttcattaac gtatcttcgt attttctata agtgatagga gatactgttc
    52021 cttctttata aagttgcatc caatcctcaa agtaatctga taataataga tttttgcgat
    52081 cccctttaag gcctttgttc aagttatatt ctaattcgtt ggcggcatct tttgcttctc
    52141 cttttgtctt aaatcctgat tttcttatct tcgtgtattt tccgtcatct tttttgtatg
    52201 aaatttcgta ttgccaacta tttccacgtt tgactaatct tgccataatt gaatcagctc
    52261 tctttctctg atacaatagg aactataaag aagcccattg tttgggtttt attatttttt
    52321 gcacatcctt tttgcttgcc ggcgcaagga tgtgctattt gtcgttttaa tggtataatt
    52381 ggatgtgtat tagatcatcc aatcagaggt gtttattatg attaaagtga tttttaaaag
    52441 aataactgaa cttccagagt ttagattatt tgttgattat tggtatgtct ggttttttat
    52501 tgttgcattg ctgataggtc tagatgtcat attagaaaga aaacttaagt aagagttgag
    52561 ttaataaatt gaattcctaa atgagatatt ttgtagccca tattttgttt gccgacatca
    52621 cctaatacta tggagctaat cgttttttct aacagaccaa aatcagataa tctatttaag
    52681 ctaccatcta ttagctcttg tgaatagcca gacatgctga tatttccagt gaaattttcg
    52741 tttccacgac tcattaagca tgctaacaat tgaatctcaa tcgatgttaa ttgattcagt
    52801 acaagaagat aatactcctc gttatcccat gaagcgttgt taattcgaag taaacaattc
    52861 ttataaagat ttttatacat atctatttta ttttgtgcct ttgatttttc tacctcgtca
    52921 tttatccctt cgaatattcc aatgagttgg tccttgttct ctaagttgaa aacatcttct
    52981 ggaatttgat cgcgaatgtt ttctaatgat tcgtttaaat ttttgtagaa tttctcgatg
    53041 cgtttgaaac gcttctcgtt tttagaccca aaataaagtg actgcacaac gccaccaata
    53101 taaggaattg ctccaatacc gccttcaact attaattcaa gatattcttt tttggttagt
    53161 ttttcattgc tattatccat agttacctcc tattttgtca tttcctttac aagcattgtg
    53221 actcctgatg gcactctttt gtataggttt tattattgtg cacaatctaa ctcttagtgg
    53281 ggcgtggttc atttattttt tacttacttt actattaatc atatcccatt gagcatagga
    53341 tttttctatg ttcgatcctc ttgaactaat ttctcctgta aaagttactt tatcgccctt
    53401 tgtaaaggta tcagcattgt ttaagtcctt cgcaaaaatt acatatgaaa ctttaggtgt
    53461 tgcagaaata tcattccaac ttttatcatc gtactctttg tctgctataa tggctattct
    53521 agtagtcatt gattcgataa cagttccttc aaaagtatat gttttaccac taatatattt
    53581 agtaaaagct gtattttgct cagtagctgg agtaatagaa taataactat ctaagaaagc
    53641 accccagtca tgattatttg tctctaaaaa ttcttcaaat tgagttaggc ttttattgct
    53701 gctgttagat gtagtagttt gagtatcaga tgatgagctt tcctgtttgt tattgtcatt
    53761 tgcctcgttt gatgaggaaa gcgagaaacc tacggtagta aaaataatcc cgataataag
    53821 gagaatcata ggtgttttct tatttgtttt tttgataaat cttacaatta acatgattaa
    53881 acctataata aataaaacaa taccagtaaa accaaatata ccgtccatta atagatcctc
    53941 tttctctttt gatattgaat attataaacc aagcaattct tttttcttaa aatcgaattc
    54001 ttcttgatta ataatccctt catctaacaa ctctttatac tttttaattt catctgcact
    54061 tgaaatttgt gtttctttgg aattattatc taatatagat tgttgcttct ctatatattt
    54121 ttttaaattt tccatttcat tccaatattt ttttgtaaac ataacggtgt tttcatcttt
    54181 cgtggccgct aatatacctc cgcgactttc gtttccgcct agcaaggtaa attgtatata
    54241 accattactc atacctggtt tttttaattg tacggctgat atatttttaa aggggatagt
    54301 tttttctcct ttaatacctt gattaactaa atttataaat ccttttctaa taattgaaat
    54361 agaatcatca gttactttaa tcaaggtttt tcctggagat tttattaaaa tctctttttc
    54421 cattttttct tcctcatttc tgtgatatga ttttttttgt agaagctcat atatgaggaa
    54481 agagcccgtg ttgcagcacg ggctttttta atgttttgct gaaatcggtt tttttaataa
    54541 ttcttgatgt attcatacat attgccttgc gtaagaatat tttttcttaa aatagcattg
    54601 gcagacagca taacaagtgt atctgagctt attctgaata gaataatact ccataaattt
    54661 ttcgagatta aactgagatt catcagtcag ttcgttctca atatagatat ttaataaaat
    54721 tagaatagct attttatctg cttctgtttc aaattttgag tgaaaagttg tagaagtatc
    54781 gtacaaaact gaaaattcaa aatgagaggc gctgaaatgt gcaagttcat gagataaatg
    54841 aaaggcttct gcagtttcac cgtatagatt ttcattcaaa aaaatgattc tgggttttgg
    54901 atagtagaaa ccaggctctt tcatctccat ataaactact ttcaaattgt attcgctcaa
    54961 catttctttc aatttcaaat acatacaaac catcactcca actattcatt ttcctctaaa
    55021 gctttagcaa ttgcaatcgc tttacgcatt gtctccttag atatttcttt tccatcaaaa
    55081 gaaaaaacag tatcgtcttc tgataaatcc acatgtttag gggtttctct ttcttctcta
    55141 cctagaaggt agtctacaga gacatcgaaa tagtcagcaa tccgacttaa ttcatctgaa
    55201 ttaggagtat tatttttcca ctttgctaga taaccgttag aatatccaaa attaatttct
    55261 aactgccgta tagatatctt tttcctcttc gccaagtctt ttattatttc ataggtattc
    55321 atcgataaaa caacctttct aaatgtttac aaaaaaagtt tagaaaaata cgcagaaata
    55381 atttgactaa ttctgagtaa tgagctatac tatatcttgt aaacagattt aacaactaaa
    55441 aagacaacaa aaaacactat tgatatgtaa atgcagaccg ccaagaaagc tttaaaatca
    55501 atgtttttat gtcttattta attatgctct tagtatagag tattactcag atactgtcaa
    55561 ttgaatttag aaaaaagttg ttaaatttgt ttacgaatat aaaagaaagg aatgagaaat
    55621 atgaacacac cgcaaatttt taatttcgaa caaaacgaag ttcggacaat tttagtaaat
    55681 gatgaaccat attttgtagg caaagacgtt gcgagtgttt tgggttattc aaacactaaa
    55741 gatgctttgt cgcggcatgt agatttggaa gataagatgg ggtcgcgaat cacgacctca
    55801 ggtcaatcaa gagagatgac aatcatcaac gaatctggtt tgtacagttt aatcttaaaa
    55861 tcaaaacttc cctctgccaa aaaatttaaa cgttgggtaa caagtgaagt gttgccagca
    55921 attagaaaac atggaggtta tctaactcca gaaaaagtag aagaagcttt gcttaatcca
    55981 gatacaatca ttcaattagc aactcaacta aaagaagaaa gaactggaag attaatcgca
    56041 gaacaaaaga ttgcagagta cgaacctaaa atctcctatt tagatagcat attatcttct
    56101 acagattcag taacaattag tcagattgca gcagattatg ggatgtctcc acaacagatg
    56161 aataaattac ttcataaact aggtattcag aaaaaagtcg gtaaccaatg gttattgtgc
    56221 aaaaaacaca tgaaccaagg atacacaaaa tctcatacaa ctgagatccc gaaagccgat
    56281 ggtggcacta aaattgtaat gaataccaaa tggacacaga aagggcgtct atttatctac
    56341 gaattactaa aaaaagaagg atattaccct caaatggatt tagaggaaat tggttagaaa
    56401 ggagttttag tatgactgac attgcagaaa tcactcaacg agatagagaa aaaatcaaag
    56461 aatatgtcga aagttcaaag ttcttaactt acaccatgct tgctgaaaga tttggaatta
    56521 gcaaaagcta cttatcttta attttaaacg gtaaaaagac ttctgcagaa gcaaacagaa
    56581 ttatagattc gattatcact atgtacgaat tgtaaaggga ggaacagcta atgcaatatc
    56641 tagaagcaaa aatcccaatt ccagaaggct atgtaattat ctcccaagtg gattatgagg
    56701 agttaaaaaa agctgatgat actggtagat ggatgacgtt gccagaagtg ctagaacgga
    56761 ttaacagaaa atatgattgg tttacttcta gagttttaaa gaacccaaga tatagaaata
    56821 ttatcgatat cgagaaaaac aaaaatggat ttgtctatta tccagttgaa ggaagagaca
    56881 catatctatt tttaagaagt aaaacacttg aatttttaga aacaaatttt tcggaaatct
    56941 taaggaggta agcggatggg taaatttaat agagcactag tattcagcgc accactaatt
    57001 atctacgctt taggtctttg gggaagcaga caagcattaa tcggcacaat tgtatatatg
    57061 gtctggatct ttatggggct tgatgaagct gaatatagag cgaaaaagcc agtcgggagg
    57121 gactgactta acctaattaa ttaaaaattg taacaaataa cgcaaaaata aagctaatga
    57181 aattgtattg ttaggatcta ctccaatagt tatatttaca atttcggtta ctactagtcc
    57241 tatcaaagct gccccagttt gctctaaata ccacatttta tctttgtaga aagcaggtga
    57301 agcttgttgt ttttcagatg gttcattagt tatatctttg ttcttggctg tatttatttc
    57361 ttcaaaaagc tttatagaat catcaaggac tttcctttgc tcattaagga tatcaaaaaa
    57421 cttagcctca aactcatcag atggattaga agtgtcttta aaaagtagat tttttgtact
    57481 aacttccggt tcggaagaaa atgctttttc taattcttgg gtagctaccc ctattctgaa
    57541 gtcgtactta gacatgtcaa cggcaaattt ttctgtgatt ttagagatat cagaaaaata
    57601 aggttttggc atagtagctg ccattttttt agcaatatta aaacttggag attctaattg
    57661 tttctgtatt gcgaacaaag aaggaggaat catattagca atagatttgt tacgtttctc
    57721 gacaatttga atgagagaat cattgagtat tgcttgcgaa gctataaaag tatcttgaat
    57781 tgcaattaat tttttagctt gttctatcgc aggctgcata gcgttaacga catctagaga
    57841 aggagcaatg gtttcaccga ttaattttga agaagctatc aatttttgat aataaggagt
    57901 atctgaaata tccaattata tacaccacct ttttacctaa attataccaa aagggagaga
    57961 aaaaaatgca ataattagta attttgaaaa ataaagaagc tgtgactacg agcttacaag
    58021 tcgcagaaac attccgaaag gaacatagag cagttttaaa ggcgattgac gatctaaaag
    58081 atgggcttgc acaaaagtat gcagacctat tttgggaaga tgtgtacatc catccgcaaa
    58141 acaaacaggc ttatcgaatt gtctatatga atcgtgatgg ctttactctt gctgtattag
    58201 gattcaacaa tactcaaaaa gttttagagt tcaaattgaa atacattgaa gcgttcaacg
    58261 aaatggaaaa ggcaattcgc aaaaatacag cttttgacac aattgaagac atgatgatct
    58321 atcaattaga agaaatgaaa gatgttaaaa aagatgtttc catgcttaaa aatactatgc
    58381 gaattagcgg acaacaagag tttgaaatta agcaaaaagg aaatacgaaa gttatggaag
    58441 ttctaggggg aaaagaaagc cgagcttatg aagaaatcag caaaaaagta ttctcaaaat
    58501 tttggtctga atttaaacgt accttttcaa tcccaagata tggcgagtta cctcgtaaga
    58561 gattcgatga tgctgtttca tttattgaaa tgtggttacc agaaactgcg attcgcatgg
    58621 aaatcgatca actgaacaga caacaaagac tttttggtga tgaaaatgaa tagagctgaa
    58681 gcgctaagaa tcgggacggt aattgctaat cgctggtgga gacacaataa accaatcatc
    58741 ctaagccaac aacatattga caagcaaaaa gcatggcaac aaataaaaaa gtgactccgc
    58801 cggcaagcaa agagtcacaa agaaaacaca tcataaggag attttagcat atggaaaaag
    58861 aactttccac tctagatcaa tatttgactg atcctagttg gggcaaatcg aatatcaagg
    58921 aaacaaataa tcgaaaaatc agacgaaatc ttttgacgga tgaagaacta gcatgtgatc
    58981 aagacgattt gggaaatttt gtgagtattt gggatcatgt ctatcttatc catttatcga
    59041 ggaagtccaa aaaacctgaa tatatctatg tcatcgaaga tggcttgatt gatgcgctag
    59101 aagagtatga cagagataac ttgattgata tctcttatta cggatcaggt aagaaataca
    59161 ttgctgaaat ggaggcagaa tttgatgagt gaaaacaaag ggacaacgac ttttgaaaaa
    59221 ctttttagtc gcaagttaaa taaaatactc aagaaaaaag gaaattttga ttatttatct
    59281 tgggctcacg catgggagat tatgaaaaag aatgatccac aggcaacggt aactattaat
    59341 gagtataaac actacagagt tgtttctgga actcatcaag actttcttgt tgaggaatat
    59401 aaaccttttc ttatggatga aactgggact tatgtatctg tctcagtaac ggttaaagga
    59461 cacacggaaa ccgaattatt tcctgtttta gattatcgaa accaaccagt tgttaaacca
    59521 aatgcaatgc aaatcaataa ctcattgaag cgatgctttg tgaaagcatt ggctctacac
    59581 ggactgggat tatatgtatt tcaaggggaa gatattccaa caccacctag aatcgatacg
    59641 aagaaattaa acatgctaga gacgattcta gaagctttca atgagcagat gggtaaagat
    59701 atgaccaaaa ccttaatcga atatgttaat gagcagacag ataaattagg gctcttagct
    59761 gataacgttg aaactattga acagttaagc tatgagcaat gtgccttgat ggagcgagca
    59821 atagcagcta agagaaaaga attagataag aagtgatatg agtgtttaaa ccattaatcg
    59881 attcatattc agcggttctg aaaaagttca aaggaaaaga cataggtgca accatcaatg
    59941 aggaagtgaa cattgatcga ctaaagacga tgtatgacgg ctacgatggt gatcgagtta
    60001 ttgaaattcg ttttattgat cctagacgtt tcacagtaca gcaacgaaac ttcatctatg
    60061 cactcatagg cgatattttc atcgatacag gcatgccaac ggacttctgg aaggaattct
    60121 tttacttccg ctttgaaggt gtcacagggc gcaaaataag cctcaaagat gaatcgaata
    60181 cgactgtgag tgatgctaac gtcttagcaa atatcatctt agatttcatc tttgaacatc
    60241 atattccttt caaagaaggc tatgagattt taccagcgaa tcaagaatat tacttctaca
    60301 aatgcatcac aaaaagagtc tgttgcatct gtggcaaaac aggagctgat atcgatcact
    60361 ttgacaaagc gctaggaaga cgaaagcgca aagaagttga tcattcagag tacacatttg
    60421 cagcactctg cagaatccat cacacggaga aacacaagat aggtgtgatc aatttcaaaa
    60481 ataaatatca aatcaaaggg atcaagttaa accaggaaac aattaagaaa cttagaatag
    60541 gaggataaaa aatattggct gacaac
//
LOCUS       DAZKKW010000007        59747 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.007, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000007 DAZKKW010000000
VERSION     DAZKKW010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 59747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59747
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.007"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(97..474)
                     /locus_tag="ACG8PO_000402"
     CDS             complement(97..474)
                     /locus_tag="ACG8PO_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0205759.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            730..1458
                     /gene="purC"
                     /locus_tag="ACG8PO_000403"
     CDS             730..1458
                     /gene="purC"
                     /locus_tag="ACG8PO_000403"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFQ0205760.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            1458..1712
                     /gene="purS"
                     /locus_tag="ACG8PO_000404"
     CDS             1458..1712
                     /gene="purS"
                     /locus_tag="ACG8PO_000404"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HFQ0205761.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            1714..2385
                     /gene="purQ"
                     /locus_tag="ACG8PO_000405"
     CDS             1714..2385
                     /gene="purQ"
                     /locus_tag="ACG8PO_000405"
                     /EC_number="3.5.1.2"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HFQ0205762.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            2386..4608
                     /gene="purL"
                     /locus_tag="ACG8PO_000406"
     CDS             2386..4608
                     /gene="purL"
                     /locus_tag="ACG8PO_000406"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HFQ0205763.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            4593..6032
                     /gene="purF"
                     /locus_tag="ACG8PO_000407"
     CDS             4593..6032
                     /gene="purF"
                     /locus_tag="ACG8PO_000407"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFQ0205764.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            6055..7107
                     /gene="purM"
                     /locus_tag="ACG8PO_000408"
     CDS             6055..7107
                     /gene="purM"
                     /locus_tag="ACG8PO_000408"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFQ0205765.1"
                     /translation="MNSMANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGF
                     GGCFDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEP
                     LYFLDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFA
                     VGIAEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKK
                     TLGEELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSW
                     PILPIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVEESKVKEIVHEIEQAGEKVYEIGK
                     VIAKEKHRVVFIGEER"
     gene            7104..7682
                     /gene="purN"
                     /locus_tag="ACG8PO_000409"
     CDS             7104..7682
                     /gene="purN"
                     /locus_tag="ACG8PO_000409"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFQ0205766.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            7679..9214
                     /gene="purH"
                     /locus_tag="ACG8PO_000410"
     CDS             7679..9214
                     /gene="purH"
                     /locus_tag="ACG8PO_000410"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFQ0205767.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            9230..10504
                     /gene="purD"
                     /locus_tag="ACG8PO_000411"
     CDS             9230..10504
                     /gene="purD"
                     /locus_tag="ACG8PO_000411"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFQ0205768.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            10626..11540
                     /locus_tag="ACG8PO_000412"
     CDS             10626..11540
                     /locus_tag="ACG8PO_000412"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HFQ0205769.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(11673..13238)
                     /locus_tag="ACG8PO_000413"
     CDS             complement(11673..13238)
                     /locus_tag="ACG8PO_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0205770.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(13231..14682)
                     /locus_tag="ACG8PO_000414"
     CDS             complement(13231..14682)
                     /locus_tag="ACG8PO_000414"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFQ0205771.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(14696..15712)
                     /locus_tag="ACG8PO_000415"
     CDS             complement(14696..15712)
                     /locus_tag="ACG8PO_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0205772.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            15932..17020
                     /gene="chvE"
                     /locus_tag="ACG8PO_000416"
     CDS             15932..17020
                     /gene="chvE"
                     /locus_tag="ACG8PO_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     substrate-binding protein"
                     /protein_id="HFQ0205773.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMISELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            17074..18600
                     /gene="mmsA"
                     /locus_tag="ACG8PO_000417"
     CDS             17074..18600
                     /gene="mmsA"
                     /locus_tag="ACG8PO_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     ATP-binding protein"
                     /protein_id="HFQ0205774.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            18602..19807
                     /gene="mmsB"
                     /locus_tag="ACG8PO_000418"
     CDS             18602..19807
                     /gene="mmsB"
                     /locus_tag="ACG8PO_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     permease"
                     /protein_id="HFQ0205775.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            20064..21041
                     /gene="guaC"
                     /locus_tag="ACG8PO_000419"
     CDS             20064..21041
                     /gene="guaC"
                     /locus_tag="ACG8PO_000419"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HFQ0205776.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            21305..22729
                     /locus_tag="ACG8PO_000420"
     CDS             21305..22729
                     /locus_tag="ACG8PO_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFQ0205777.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            complement(22771..23028)
                     /locus_tag="ACG8PO_000421"
     CDS             complement(22771..23028)
                     /locus_tag="ACG8PO_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0205778.1"
                     /translation="MPIRILTALVNKITIRKAFYISSAFFLFSNQTYNHLFLFKNVGV
                     IQFCCKHLFYLHIVNGRTNNGFFLVSLYRILFPKKKKQLEI"
     gene            23190..24299
                     /gene="pdhA"
                     /locus_tag="ACG8PO_000422"
     CDS             23190..24299
                     /gene="pdhA"
                     /locus_tag="ACG8PO_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HFQ0205779.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            24302..25279
                     /locus_tag="ACG8PO_000423"
     CDS             24302..25279
                     /locus_tag="ACG8PO_000423"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HFQ0205780.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
     gene            25304..26947
                     /locus_tag="ACG8PO_000424"
     CDS             25304..26947
                     /locus_tag="ACG8PO_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HFQ0205781.1"
                     /translation="MAYQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAAESQTPAKPAA
                     EPTVDTESAGSSSEGVFQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSAEAPKEKVETS
                     GSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKEDIENFLAGGPS
                     SAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPTRKAIAKAMVN
                     SKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALTATVKKYPVLN
                     ASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEINEKAKLAHDG
                     KLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIVNAEGEIVVGR
                     VMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            26955..28361
                     /gene="lpdA"
                     /locus_tag="ACG8PO_000425"
     CDS             26955..28361
                     /gene="lpdA"
                     /locus_tag="ACG8PO_000425"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFQ0205782.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            28523..28798
                     /locus_tag="ACG8PO_000426"
     CDS             28523..28798
                     /locus_tag="ACG8PO_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HFQ0205783.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            28804..29571
                     /locus_tag="ACG8PO_000427"
     CDS             28804..29571
                     /locus_tag="ACG8PO_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HFQ0205784.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            29739..31568
                     /gene="typA"
                     /locus_tag="ACG8PO_000428"
     CDS             29739..31568
                     /gene="typA"
                     /locus_tag="ACG8PO_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HFQ0205785.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            31829..33601
                     /gene="recQ"
                     /locus_tag="ACG8PO_000429"
     CDS             31829..33601
                     /gene="recQ"
                     /locus_tag="ACG8PO_000429"
                     /EC_number="5.6.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HFQ0205786.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            33715..34017
                     /locus_tag="ACG8PO_000430"
     CDS             33715..34017
                     /locus_tag="ACG8PO_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HFQ0205787.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            34020..35183
                     /locus_tag="ACG8PO_000431"
     CDS             34020..35183
                     /locus_tag="ACG8PO_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFQ0205788.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKNFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            35209..38637
                     /locus_tag="ACG8PO_000432"
     CDS             35209..38637
                     /locus_tag="ACG8PO_000432"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004736 - pyruvate carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009374 - biotin binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HFQ0205789.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            38658..39782
                     /locus_tag="ACG8PO_000433"
     CDS             38658..39782
                     /locus_tag="ACG8PO_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP-associated domain-containing protein"
                     /protein_id="HFQ0205790.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            39763..40209
                     /locus_tag="ACG8PO_000434"
     CDS             39763..40209
                     /locus_tag="ACG8PO_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFQ0205791.1"
                     /translation="MIAVIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESA
                     KVCQLKEAFLSKKEAFENVAAYGEYAPDYREKKRVVRKAKRQLDMDEHVAAFRYSETQ
                     LQSLLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            40193..40531
                     /locus_tag="ACG8PO_000435"
     CDS             40193..40531
                     /locus_tag="ACG8PO_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HFQ0205792.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            40650..41210
                     /gene="rsmD"
                     /locus_tag="ACG8PO_000436"
     CDS             40650..41210
                     /gene="rsmD"
                     /locus_tag="ACG8PO_000436"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HFQ0205793.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            41207..41698
                     /gene="coaD"
                     /locus_tag="ACG8PO_000437"
     CDS             41207..41698
                     /gene="coaD"
                     /locus_tag="ACG8PO_000437"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFQ0205794.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            41688..42731
                     /locus_tag="ACG8PO_000438"
     CDS             41688..42731
                     /locus_tag="ACG8PO_000438"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SepM family pheromone-processing serine
                     protease"
                     /protein_id="HFQ0205795.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            42890..43618
                     /locus_tag="ACG8PO_000439"
     CDS             42890..43618
                     /locus_tag="ACG8PO_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFQ0205796.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            43734..43979
                     /locus_tag="ACG8PO_000440"
     CDS             43734..43979
                     /locus_tag="ACG8PO_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFQ0205797.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            43976..44194
                     /locus_tag="ACG8PO_000441"
     CDS             43976..44194
                     /locus_tag="ACG8PO_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFQ0205798.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            44330..44869
                     /locus_tag="ACG8PO_000442"
     CDS             44330..44869
                     /locus_tag="ACG8PO_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HFQ0205799.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            44862..47135
                     /locus_tag="ACG8PO_000443"
     CDS             44862..47135
                     /locus_tag="ACG8PO_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HFQ0205800.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            47173..48219
                     /gene="holA"
                     /locus_tag="ACG8PO_000444"
     CDS             47173..48219
                     /gene="holA"
                     /locus_tag="ACG8PO_000444"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFQ0205801.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(48308..48562)
                     /gene="rpsT"
                     /locus_tag="ACG8PO_000445"
     CDS             complement(48308..48562)
                     /gene="rpsT"
                     /locus_tag="ACG8PO_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFQ0205802.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(48962..49231)
                     /locus_tag="ACG8PO_000446"
     CDS             complement(48962..49231)
                     /locus_tag="ACG8PO_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0205803.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(49493..49621)
                     /locus_tag="ACG8PO_000447"
     CDS             complement(49493..49621)
                     /locus_tag="ACG8PO_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0205804.1"
                     /translation="MFEKLKYFIGTIGWFLLAVLLILGLAYLLFPDLIQNVFDTAF"
     gene            complement(49701..50156)
                     /locus_tag="ACG8PO_000448"
     CDS             complement(49701..50156)
                     /locus_tag="ACG8PO_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HFQ0205805.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(50294..52222)
                     /locus_tag="ACG8PO_000449"
     CDS             complement(50294..52222)
                     /locus_tag="ACG8PO_000449"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFQ0205806.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            complement(52419..53252)
                     /locus_tag="ACG8PO_000450"
     CDS             complement(52419..53252)
                     /locus_tag="ACG8PO_000450"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFQ0205807.1"
                     /translation="MFFANIIKAVILGIIEGITEWVPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            complement(53444..54148)
                     /locus_tag="ACG8PO_000451"
     CDS             complement(53444..54148)
                     /locus_tag="ACG8PO_000451"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFQ0205808.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            complement(54171..54797)
                     /locus_tag="ACG8PO_000452"
     CDS             complement(54171..54797)
                     /locus_tag="ACG8PO_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFQ0205809.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            55123..57498
                     /locus_tag="ACG8PO_000453"
     CDS             55123..57498
                     /locus_tag="ACG8PO_000453"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HFQ0205810.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            complement(57522..58013)
                     /locus_tag="ACG8PO_000454"
     CDS             complement(57522..58013)
                     /locus_tag="ACG8PO_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HFQ0205811.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            58200..59120
                     /locus_tag="ACG8PO_000455"
     CDS             58200..59120
                     /locus_tag="ACG8PO_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFQ0205812.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            59274..59618
                     /locus_tag="ACG8PO_000456"
     CDS             59274..59618
                     /locus_tag="ACG8PO_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HFQ0205813.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
BASE COUNT        19621 a        10324 c        12842 g        16960 t
ORIGIN      
        1 ttttagttta ttatattttc ttatcaataa taggaacctg attttaaaag cggctacctc
       61 tttaccgtgc ctctttacat acaaaaaata gaggaattaa gcgtaaatgg cttggctatt
      121 ataactaaaa tgctggttat ggagatgttg cagcaagttt tcgagaaaag cgtattccat
      181 ctcttgtcca ttgaataaga tctggatacg ataatgattt ttgaaacgct tctgttcatg
      241 aatctgactg attgaaagtt cgcctttgtg ttctgtctga taaagaaaag tccttttacg
      301 gcattcagca cagcgcttgg ttgctcggcg gatttttcgg aatgtataaa aactagtatc
      361 tttattgcat aaataaacgt ctttgatcga cagcaaatca ttatatagac aggaacgttc
      421 ttcagctgtt aattctgata aaaaaagctg accttgtttt attgcatctt ccatcggcaa
      481 gtgcctcctc gaacccatta tccaaatttt tagttaattc taatatacca tcattaagta
      541 taaatgtaaa cgcttgttga tggtataaga ttttcgtttc tgataaaaca cgaacattaa
      601 aagaatctaa tgccttaaat gtacatattt ttgttgattt ataaaaagat gattgctaaa
      661 atttaaagtg aaaaaacgaa caaaaaacga tgtatttgct tttaatgtaa ataaaaagga
      721 gagacgtaca tggagaagaa agaacttttg tatgaaggaa aagccaaaaa aatatatctt
      781 acttctgatg agaacttgat ttggatagaa tatttagatc aagcaactgc gttgaatggc
      841 gcgaggaaag accagataag tggaaaagga gaattgaata atcaaatcac aagtgcaatt
      901 ttccgttatc tgactcaaag aggaataaaa aatcatttta ttgaacaatt gtctaaaacc
      961 gaacaattag caaaaaaagt acaaattatc cctctggaag tggttatccg aaatattgca
     1021 gcaggaagtt tctcaaaacg tctaggtgtt cccgaaggta aagaattcat caaaccaatc
     1081 attgaattct actataagga tgaccagttg gatgatccat ttatcaatga agagcatatc
     1141 tatttcttac aacttgcaaa gccggaagaa atcagtgagc tgaaagccca agcgttggct
     1201 attaatgaaa ttttgaccga attatttgct aaggtcggtt tggttttagt agatttcaag
     1261 ctagaatttg gccggtcgaa tggacaaatt ttgcttgcgg atgaagtaac accggatact
     1321 tgccgattat gggatgaacg tacgaatgaa catatggaca aagacgttta ccgcagagat
     1381 ctaggtgatt tgatcccagt ctatcaagaa gtatataacc gtctatcgga actaactgaa
     1441 gaaaaggagc gagtatgatg tatttcgtaa aagtatatgt aacctataaa gattcagtgt
     1501 tagatcctca aggggaggca gtaaaaaaag cagtccatcg tatgggatat gaaacaatcg
     1561 aagatatccg aataggaaaa tactttgaga tacaacttgc tgatgcagaa gaaccaatcg
     1621 atacagtgat tgaaacgatt tgtgacaagc tgcttgcaaa tgtaaatatg gaaacatacc
     1681 gatatgaaat caaaaaaatg gaggaagtat aacatgaaat ttgcggtcat cgtttttcca
     1741 ggttcaaatt gtgacgcaga tatgttatgg gcagttcgcg agatcatggg cgctgatgcc
     1801 gaatttgtac ggcatgatga gtcctcttta gaaggatttg atggggtttt actgccggga
     1861 ggattctcct atggtgacta tttgagatgc ggtgcgattg ctcggttttc tacaatcatg
     1921 gaagaagtga ttcgttttgc tgaagaaggg aaaccagtat tcggtacatg caatggattc
     1981 cagatattga cagaagcagg acttttgcca ggtgtactga gacgaaatga gtctttgcat
     2041 tttatttgca aaactgtacc attgaaagtc aaccccaaga cggcctttac ttcagagtat
     2101 gaagccgacg aaataatcta tcttccagtt gctcatgggg aaggaaatta tttctgtgac
     2161 gaagaaacac ttcaagaact aaaaaaaaat aatcagattg cctttactta taatgacaac
     2221 atcaacggca gcgtggaaaa cattgcaggg attacgaaca aagcagggaa tgtgttagga
     2281 atgatgcctc atccagaacg tgcagtagaa gacttgctag gatcagaaga tgggaaacgg
     2341 ttctttgctt ccattttaaa aaacttcgga aaggtgaccg tatagatgaa aacattgaaa
     2401 gaaccgacag ctgaagagat caagaaaaaa agaatctatg ccgaatgggg attgactgat
     2461 gaagaatatc gaatgatcga agaagatatc ttaggaagaa tgcctaatta tacggaaact
     2521 ggactgtttt ctgttatgtg gagcgaacat tgttcctata aaaattctaa accggttcta
     2581 cgcaaatttc ctactagtgg acctcatgtg ttgcaaggcc ctggagaagg cgcaggcatc
     2641 gtcgacatcg gtgatggact agcagttgtc ttcaaagcag aaagtcacaa tcatccttca
     2701 gccgtggagc cttatgaagg agctgcgaca ggagttggcg gaatcatcag agatattttc
     2761 agcatggggg ctcgtccaat tgcgttgcta gattcattga ggttcggcga attagataat
     2821 ccaagaacga aatatttact agaagaagta gttgcaggta tcggcggata cggcaattgt
     2881 atcggcatac cgactgtagg cggcgaaatt gcatttgatc cttgttatga aggaaaccca
     2941 ttagtcaatg caatgtgcgt aggtttgatt gaacacaaac atatccagaa agggcaagcc
     3001 gctggcgtcg gtaattcgat catgtatgta ggtgcaaaaa caggtcgcga cggtatccac
     3061 ggagcaactt ttgcctcaga agaatttaat caagaagaag aacagcaacg ttctgctgta
     3121 caagtaggcg atccttttat ggaaaagctg ctgcttgaag cgtgtttgga attaatcttg
     3181 catcattcgg attctctaat aggtatacag gatatgggag cagcaggact tgtttcatct
     3241 agtgcggaaa tggcttcaaa agctggctca ggacttatat tgacattaga tgaagtccca
     3301 caaagagaaa ctgggatgac accttatgaa atgatgttat cggaatcaca agaacggatg
     3361 ttgatttgtg ttaaatcagg agaggaagaa aaaatccaag aactatttca gaagtatgat
     3421 ctagatgcag tcactatcgg taaagtaaca gacgacgggc aatatcggct atatcatcaa
     3481 ggagaagaag tagcaaatct gccagtcgat gcattagctg aagatgcacc agtttatcat
     3541 aaagaaatga aagaaccagc tcgaatcgta gaatttcagc agttagctcc ttatcagcca
     3601 gttatcgaag aacctggaca agtattgctt gatctgctgc agcaaccgac gattgcttca
     3661 aaacgttcaa tctacgaaac ttatgattca caagtacaaa ccaatacagt tgtccgtcca
     3721 ggaagcgatg ctgctgtgat gagggttaga gggacgaata aagcgttagc tatgaccaca
     3781 gactgcaacg cgcgctatct gtatttggat ccaaaaatcg gaggacaaat agcagtggca
     3841 gaagctgcta gaaatattat tgctagcggt ggtaagccct tggcgatcac cgactgcctg
     3901 aactatggtt ctcctgataa accagaagtt ttttgggaat tatcgacttc agctgacgga
     3961 attgctgctg cgtgtgaaac gttaggaact cctgttattt caggaaatgt ctctttatat
     4021 aatgaaacgg atggtcaagc aatctatcca acaccaatga ttggtatggt cggattgatt
     4081 gaggatcata aacatatcac tactcaggaa ttcaaaaaat caggggatct aatctatata
     4141 ttaggtaaga cgtttgcaga ttttgatggc agtgaattac aaaagatgca gcttggaaga
     4201 atcgaaggtg tgatccgaaa ctttgatcta tccattgaaa agagaaacca agagttagta
     4261 ttgacagcca tccaaaacgg attgattgaa agtgctcatg actgttcaga aggtggcttg
     4321 gcaattgcac tggcagaatc agctttcaaa catcagctag ggatatctgt acagtttgaa
     4381 ttgtcctctg ctcaattatt tgcagaaact caatcaagat ttgtgttgac agtcgcaccg
     4441 gaaaataaga cacgattcga agaaatgatg ggagatgctg ctgttttagc aggtaaggtg
     4501 acggatgaag cgataatcga aatttcagca actgacggtc agatcaaaat agaaactgca
     4561 gtagcaagaa agtgctggga ggatgcaatc gtatgtctta tgaagtgaag agtttaaatg
     4621 aagaatgcgg gatcttcggt gtttggggtc atccagacgc cgctagtgtg gcatacttcg
     4681 ggctccatag tcttcagcat cgaggacaag aaggtgcagg tatcgtgtcc aatgatcgag
     4741 gaaagcttaa ggggcatcga gatttggggc tgctttctga ggtattcaaa gatcaaagaa
     4801 aattgcagca gttaacagga gaagctgcaa tcggacatgt acgttatgca acagcaggca
     4861 atggaagtgt ggacaatatt cagccttttc tattcaaatt tcatgatcag tcgatcggct
     4921 tagcgcataa tggtaacttg atcaatgctc gttcccttag aaaagagtta gaggaaaatg
     4981 gggcgatctt ccattctaac tccgatacgg agatattgat gcatttgatc cgacgaagtg
     5041 aagaaacaga ttttttaaat aaattaaaaa gtgctttgaa tgaagtcaaa ggtggctttg
     5101 cctatttatt aatgacagaa acggcaatga ttgccgcttt ggacccaaat ggttttcgtc
     5161 cattagcaat cggacaaatg aaaaacggag catacgtgat tgcctcggaa acttgtgcat
     5221 tagaagttat tggggcacgt tttataagag atgtccagcc tggtgagata gtgatcatca
     5281 atgacgaagg aatccagatt gattgcttta cgaaggacac gcagctttct atttgttcga
     5341 tggaatatat ctattttgcc agaccagatt caaacatcgc agggatcaat gtgcatactg
     5401 cacggaaaaa tatgggacgg aatttagcga aagaatcgcc agtaaaagca gatatggtcg
     5461 ttggcgtacc taattcttct ttgtcagccg caagcggtta tgcggaagct agtggaatcc
     5521 cttatgaaat cggattagta aaaaatcaat acattgcccg aacatttatc cagccaacgc
     5581 ctgagctaag agagcaaggt gttcgaatga aattatctgc tgtacgagga gtagtggaag
     5641 gtaaaaaggt gattatggtc gatgattcaa ttgttcgggg tacgacaagc agacggatcg
     5701 tccatctttt gaaagaagca ggggcaaaag aagtccatgt gaggattgct tctcctcctt
     5761 tgaaatatcc atgtttttac gggattgata ttcaaacgcg taaagaactc attgcggcaa
     5821 atcacacgat tgatgaaatc aaagaatgta tcggtgcaga ttctttaggt tttttaagtg
     5881 aagagggtct gatcgaatcg atcggtctgg aaaaagatgc tccgtattca ggattgtgta
     5941 tggcttattt caacggagat tatccgacgc cgttatatga ctatgaagaa aaatatcagg
     6001 aatcactaaa agaaaaagta tcgttctttt aaaagcagac caagaaggga gtatatgaat
     6061 tcaatggcaa atgcatacgc aaaagctggc gtagatgtcg aagctggata tgaagtagta
     6121 gaacggatca agaagcatgt caaaaaaacg gaacgtttag gtatgatggg cgctatagga
     6181 ggattcggcg gttgttttga tttatcggtc tttcaattaa aagagccagt ccttgtctcg
     6241 gggaccgatg gcgtggggac gaaattagca gtggcaatcg aagcagacaa acacgataca
     6301 atcgggatcg actgcgtagc gatgtgtgtc aatgatatct tagcacaagg cgcagagccg
     6361 ttatatttct tagactactt agcaacgggg aaaaatgatc cgttacgttt ggaaaatgta
     6421 gtaaaaggtg tagcagatgg atgtgttcaa ggaaatatgg cgctgattgg cggagaaaca
     6481 gctgaaatgc ctggtatgta ctcaggtaat gattatgatc tagccggatt tgctgtaggg
     6541 atagctgaaa aatctcaatt attgacagga gaaacgatca acccaggaga tgtgttgctt
     6601 ggaatagcat ctagcgggat tcattcaaat gggtactctc ttgttcgcaa aattttcttt
     6661 gagcagcagc agttttcttt atcatcaaaa ctgccagaat tgaaaaaaac acttggcgaa
     6721 gaattgcttg agccaacgaa aatctatgtt tcttccttgc ttccgctgat caaagaaaag
     6781 aaaataaaag gagctgcaca tatcactggt ggagggtttg tagaaaatat cccgcgaatg
     6841 ttaccagaat ctatggctgc acaaatcaaa cttggctcat ggccgattct tccaatattc
     6901 ggctgtttac aaaaatatgg tgaaattcct gagatggaga tgtatgaaat cttcaatatg
     6961 ggcatcggta tggtcctggc agtcgaagaa tcgaaagtaa aagaaatagt acacgaaatt
     7021 gagcaagctg gcgaaaaagt ttatgaaatc gggaaagtga tcgccaaaga aaaacatcgt
     7081 gtggtgttta taggagaaga acgatgagaa tcgctgtttt tgcttctgga aacggaagca
     7141 actttcaagc gctagccgat tatctatcga aaaaaggatt ggaatcttcg attgactggt
     7201 tgttttgtga tcagcctgaa gcatatgttt tgaaacgtgc aactgcgttg agtgtaccgg
     7261 cagattgttt ttcacctaaa gaatttgatt caaaaaaaga gtatgaagaa gcaattttgc
     7321 ataaattgaa agaaaaaaag atcgatttga tcgtattagc tggatatatg cgaatcattg
     7381 gaccggtatt gctgaaaaat tatgataaaa gaatcatcaa tatccatccg tctcttttac
     7441 ctgccttccc tggtttgcat ggcatacgtg atgcctttga agctggagta aaagaaacgg
     7501 gtgtcacaat tcattatatc gaccaaggtg tagatactgg accaatcatc cggcaggaaa
     7561 aagtgagaat tgaacaggag gacacgttcg attctttaga agaaaaaatc catagagtag
     7621 agcatcgaat ctatccagaa gtgattagtg aaattattga aaatggaggc tatctgaaat
     7681 gacaagagca ttgatcagtg tatcagataa aactgggatt gtagcattcg cccaaggttt
     7741 gattgaacaa ggagtagaaa tcatttcaac aggaggaaca aagcatgcgt tggataaagc
     7801 tgggatcaaa acgctttcga tcgaagaagt gacagggttt cctgaaatga tggatggccg
     7861 tgtcaaaaca cttcatccta aaatccatgg tggtctttta ggaagacgcg atctccctag
     7921 ccatatggag gcaatggaaa cggaaaatat ccaaccaatc gactttgttt gtgtcaattt
     7981 atatcctttc aaagaaacga ttatgaaacc agatgtcgaa atagcagaag ctattgaaaa
     8041 cattgatatc ggtggtccta gtatgctccg tagtgcagca aaaaaccatg aatttgtcac
     8101 ctctgttgtt gatccatctg actatgaaga agtactttca gaattacgtc agtatgggca
     8161 tacttcactt tctttcagac aaaaactagc ggcaaaagtc tttcgtcata ctgcggcata
     8221 tgatgcactg atcgctcaat acttaacgga ttgggtggaa gaaaaagaac cggaaaaatt
     8281 gacacttacg tatgaacgaa aacaaacgct tcgttacggt gaaaatagcc atcaagccgc
     8341 tgccttttat cagtccgctc ttctggaaaa atattcgatc gcagcagcaa aacaactcca
     8401 tgggaaagaa ctttcttata ataatatccg agatgcagat gctgcgctac ggatcatgaa
     8461 ggaatatgac gagccaacgg tagtcgcctt gaaacacatg aatccttgtg gtattgggac
     8521 agcatctacg attttagaag cgtgggaagc agcgtatgaa gcagatcctg tttcaatctt
     8581 tggtgggatc atcgttttga atcgagaagt agatctgcca acagcagaac agatgcataa
     8641 gctgtttcta gaaatcatca ttgccccttc ttatacacaa gaagctttgg aattactgca
     8701 aaaaaagaaa aacatccgat tactgacagt agactttgcg caggaagaaa atcaaaatcg
     8761 agaagaaacg gtctcagtct taggtggact tcttgtacaa gatcaagatc tggcaataga
     8821 aaatgaagaa gagtggaaag tcgtgacaaa acgtaagccg actccagcag aagcaaaagc
     8881 attagcgttt gcttggagag ctgtcaaaca tgtgaaatct aatgcgatcg tactggcaaa
     8941 cgaacgtcag acagtaggaa tcggagccgg gcagatgaat cgagtcggtt cagtaaaaat
     9001 tgcgattgaa caagcgcaag agaaaatgga aggtgcagtt ttagctagcg atgcttattt
     9061 tccaatggat gattcggttg aatatgcagc caaacatggt ataaaagcaa tcattcagcc
     9121 aggaggaagc atcaaagacc aagattctat tgatatggcg aataaatacg atctagcaat
     9181 ggtctttact gatgttcggc attttagaca ttagagagga gtcggataaa tgaacatttt
     9241 ggtgatcggt gcaggtggtc gtgaacatgc aataagtaaa aaacttttag ccagtccgaa
     9301 ggtggatacg gtgtattgtg cgccaggaaa tccggggatg acaaaagatg gtatcaattg
     9361 tgtcgctatc agtgagcgag atcattcagc gttaatccat tttgttaaga aagaggctat
     9421 tgattggagt atcgttggtc ctgaagttcc tttgttaaat gggatcgtcg atgattttat
     9481 ggcggaagga ttgaaaatct tcggtccgac aaaagcagca gctctgatcg aaggttcgaa
     9541 aagttttgct aaacaattaa tggatactta tcaaatcccg acagctaaat ccagaagttt
     9601 tgaatgttac gaaaaagcag cagcgtatat caaagaaaat ggtgcgccaa tcgtagtgaa
     9661 agcggacggc ttagcagctg ggaaaggtgt agtcgtggct accacagtga aggaagcgct
     9721 ggatgcggca gaacaaatgt tgaaccagca tcgatttggg gatagtagca aaaaaatcgt
     9781 cgtagaagag tttttggcag gagaagaatt ctcccttttg gcgtttgtcc gtaataccaa
     9841 ggtgtatccg atggtcattt cgcaagatca taagcgggca tttgataacg ataaagggcc
     9901 aaatacaggc ggaatgggtg cttattcacc agtaccgcaa atttcaaaaa agatgattga
     9961 aaaagctgtg gaagaagttt taaaaccagc ggctttggga atgaaagaac taggacgctc
    10021 tttcacgggt gttttatatg cgggtttgat tgctacgaag gaaggaccaa aagtgatcga
    10081 attcaatgcg cgtttcggtg atcctgaaac gcaagtcgtt ctttctcggt tgaagagtga
    10141 ctttgcgcaa gtaatcgatg atttattaga gaatcgtaca gtagagttaa agtggcagca
    10201 agaaggatac aatgtaggcg ttgtggttgc agctgccggt taccccgaag aatatgagac
    10261 aggaatggtt ctaccagatt ttctggaaga agagctttct gtttactatg ctggagtttc
    10321 ctcaaataac ggcaaactag ttggagcagg aggacgtctt tatttagtag aagcatacgg
    10381 tgagacgatc aaagaagcac agaaaaaaat ttacaccgcg ttaatggata aaacgacaga
    10441 tggaacattt tatcgtacag atattggaga aaaagctgcc aatagcgaac tgatcgtgaa
    10501 gtaaatcagc tgcttccatt tataaaaaat acaacaaaca taatcggaag cgccgatttt
    10561 agtgaagcct tttcataaag agtgtggtac acttttgttg tgtatataaa taaggaggag
    10621 ttcagatgaa aaaaattcaa ttaggtactt ctgatcagaa agcagctgca gtgatcttag
    10681 gctgtatgcg tatcaacaat gcggaagatc cagtaaaagt gattgagaca gcttatgaaa
    10741 acggcattga tttttttgac catgcagata tttatggaaa cggtgaatgt gaagaaatct
    10801 ttgcagatgc tctagcaaaa acatcaatca aacgagaaga tctttttatt caaacaaaat
    10861 gtggaatcgt tccaggtgtg atgtttgatt tttctaaaaa acatatcatt gaatctgtag
    10921 aaaaaagttt gacccgtctg aaaatggatt atgtggatgc tttacttctt catcgtccag
    10981 atacattggt agaaccagag gaagtagctg aagcgtttga tgaattggaa cggtcaggaa
    11041 aagttcgtta ttttggcgtc agcaatcaaa aaccaatgca aatcgaattg ctgaaaaaat
    11101 atgttcgtca accattagta gctaatcagc tgcaatttgg cttgaaacat actggaatga
    11161 tcgatcaagg gattcatgtc aatatgacag atgaagcaag tatcgatcat gacggtagtg
    11221 tacttgatta ttcccgattg catgatatga cgatccaagc atggtcacca taccagtatg
    11281 gattttttga aggtgtattt atcgggaacg aaaaattccc tgaattaaac caaacattag
    11341 aagaactagc agctaaatat caaacgacac caactggctt ggcttctgct tggattttac
    11401 gccatccagc caatatgcaa gtgatcgccg gtacgatgaa cgtagggcga atcaaggaaa
    11461 tcgctcaagc ttcagaaatt aatatgtctc gggaagattg gtacgaattg taccgtgctg
    11521 ctggaaacat tttaccttaa agattgtcat aagcaggtca aaagggctat aaattaaaaa
    11581 gacagaataa cttgaaagct gttttcagct tcgagatatt ctgtcttttt ttattgcaaa
    11641 tggaaagagt atcattgtac ggatttcgtt tattagttat ttttgttttt aaggaaaagc
    11701 tgtgtccgat attcttttgg agaaatccct tgttttttct taaataaata actgaaatag
    11761 ttagggtcat tataaccaat ttcaaaagca atatctgaaa tacgatagtc tgattgctgc
    11821 aataattttt ttgctaattc gattctttgt tctgtcagat aatcgatgaa tgtttggtta
    11881 aacgattgag aaaaaatcgt actgaaatga gagggactta atgcaacttg ttgagcgacc
    11941 caagttaagg acatatctgg atcggtaaaa ttttctttga taaaatccaa tgcatggttc
    12001 aaaattggct gatatttgtt ccttgaaggc tgtgagggca gagcagttaa gaaagaaatg
    12061 aacatataaa ctgtcgaacg gtaaagcgag tcgtcggcgc tgattgctaa gcgttgactg
    12121 gtggatgtgc aatgaaccat ttcttgcggg attttctttt ctttcaggca gacatgctgg
    12181 agtagcttag tcaagacaaa taaacgatat aatcgtgttc gagttggtgt gccttgtggt
    12241 ttcgtcaagt gttcgatgta cttagatact tcgtttttcg ataatgaaga caaatcaagt
    12301 gagaggtcaa agtaaaagac agaatcaact ggaagtttcg cttcttgaat caaatcctca
    12361 tagctgataa tttgttcttt aagggaactg gcggtttcaa gtatcatctg cgtgatctca
    12421 aaagatgcag gtaattcgct gattctttct gcgacaggac caatggcgac agaaaaatta
    12481 tttttttgtc ccttttctaa ttcatggacc aacgtttggg ctacctgata actggtctcg
    12541 atcacatttt cttcaagaga atcaactatt aaaaatttga tgaatcgaga ggaaacagca
    12601 gagaagatca cttgttgatt ctccctaaat aaaaacttca aataatcact gagatgagcg
    12661 taatcttcaa agtctgtact gacttggttt gctgctaata atacggtcat ttttttccct
    12721 gctaaagagc gattgaaaga ttcagcaagt ttcaatgttt cactgatcgt aagtgagcct
    12781 ttgaacaaac cgttcaagaa atgatttttt ttcaattcga aaacaagatc agaagaagtg
    12841 tgcgtttctt tttgctgcaa atctaaagca gttactgctt tttctattgc ttgtttcaat
    12901 tcgctacttt taatcggttt aagcagataa tcatctgcct gtacctggat tgctcctttc
    12961 atataatcga aatcgtcgaa tccactaata aatatgagac gagtccaagg aagtagtttc
    13021 cttgcttctt ttgaaaaagt caacccgtcc atgaaaggca tttggatatc agtcagaatg
    13081 atgtcgggct gtaagtcgat aattgaagac agtgccatct caccatctcc agcttcgccg
    13141 acataagtga ttggataata agaaccaagg acagttattt gttcccgcaa gttattgcga
    13201 atgaggtatt cgtcttcgat gataaaaact ttatacatgt ttgtttcctc caattgtttt
    13261 gggagataga gggatcatca gtgtgacagt tgttccttct cggtatttgc tgtcaatcgt
    13321 gataggtttt tgatcatttc cgtaataaag aaggagacgc ttgtttacgt tgtaaagtcc
    13381 gtagccgata ctgaaatctg aatcgatccc tttggctaac tctttacgta tttctgccag
    13441 tttttctgca gggatgccaa tcccgttgtc tataactttg aattggatca tctcatcaat
    13501 tttgcatgtg gtaatacgaa taaatccttt gtgacggacg tacttcgttc catggtatac
    13561 agcattttca atcaatggct gcaagatcat ttttaaaata gaaacaggaa aaagtgtttc
    13621 tggtatatcg atcttatagt caagcatatg gccgtatctt atttttaaga tgactagata
    13681 atcattgata tggctaattt ctttttcaag aggaatccag tcttgtcctt tgcttaaaga
    13741 cgtacgaaaa aagccggaaa gtgcatgggt catagtcaga gcttcttcaa agcagtcttg
    13801 ttcgatcaaa gaaataatgg catccaaact gttataaata aaatggggag taatctgagc
    13861 ttgcaataca cgaacttcac tttgtgctaa atggtattgt ttcaaagcgt tctcccgtaa
    13921 agactggttt agatcttccg ccattttatt catgctagcg gtaagtgttt gtaattccag
    13981 tgtttccggc agggttaaac gaaagccaag gttccctgaa gaaagttgtt ctgacatctc
    14041 gatcagctga ttgatcggtt cttggacctt tctctctaaa aagcgacgat tctttttcgc
    14101 atagaaaaaa atggtactaa gaactccaag ttccactatt gttaatagaa tgagcgagcg
    14161 gacaagtgtc gtattttttt tgcttgctaa gtcgatctcc acacgaacaa aatcttgtaa
    14221 gatatctgac agcagttgag tgacagaatc tacctgattg atgatctctt cattggtatc
    14281 cataaaatct tttgccaaga tattattgat cagctcgttt tgataatctt ccagtgtatc
    14341 gatcgtacgt agcgatacat ctaacgtact aatttcttta agtgtatttg tattattttt
    14401 gatctctcga atatcattcc taagttcttc taggatattt tcctcattga actcatctac
    14461 ggttacttgg ccagaaatca agtcccacat actttccaaa actttttgat caaccttcaa
    14521 agcaattgca tctgcttttt ggatatttgc gatcgtctgt tgataaatca aaagctgttg
    14581 cgaatataac agtatgctca gtagcaaagg gataacagca aatagaagca tggctttgtt
    14641 tgcttgggca aaaatctgtt ttatgcttaa attttttgtc atgatacttc tccagtcaat
    14701 aatttctggt tgggatgtta gctatattct gctgggaaaa gacagtctcg gaaataaaaa
    14761 tatctttagg taatgttttt cctgtttgaa tatttccaaa ataacgagta acggtattgg
    14821 ctacatacca gccgacatca ggtttacact cgactacaca attgacgatt cctgagcgta
    14881 gcttctcgat catatcttct tgtccatcaa tagttacaat gatcaaatct tctccaggtt
    14941 tgattgatgt ttcttttatc gcagctaaag caccgtgagt catttcatca ctatggctgt
    15001 ataatacatc gatttcggat aaatcatttg tttctaaata tcgtttcatc tggtcttttc
    15061 ctttcatccg aataaaatca ccgacaattc ggtgaacaat agtcatcgga ggtttatggt
    15121 cgatggttcc atcttttttt agggttggtt tacgattgat tgattccaaa aatccttcgg
    15181 agcgtagctt ggtcggtgtg gagttctctg aaccagcaag ttctaggatt ctgatttcct
    15241 gtttctttga atttgtgaag tgattgctga caaataaacc cgcccgattt ccttctgctt
    15301 tgaaactggg gccaatgtgg gtgacgaaaa gctcgggatc attcgtttga atatgtctgt
    15361 cgacaagtat gacagggatc cctgctcgct ttgcttcttt taatacagag tcccacccgg
    15421 attcttgcaa aggagtaaaa atgatcatat ccactttata ggcaatgaac gtccgtatat
    15481 cttggatttg ccgttcctga ttcatgtagc catttcggta gagaacttgg taatcttctt
    15541 tttcaagcgc agtgataatt gattcagtat gctttttccg ccagctactc tccgttcctg
    15601 attgcgaaaa accaacgact agtgggtcag gtgcttcctt tgtattaggt gtacacccca
    15661 tcatttggta agcgattaca acaagggcta aaaaaaataa aacacgtttc actttaaatc
    15721 actccatctg gatttgttag tactatctta tcagataaaa gggaaaatca aagtcaaaag
    15781 gaatcaatcg aaaaaaatcc aagaaactta aaaaaatcca agacaaaaaa agtacaccta
    15841 aaaaatttaa agtaaatccc ataaaaaaat caatgttaac gctttcttta ttagctatac
    15901 tcggtgtatc aattgaaatg ggggataaaa aatgaaaaac aaatttagaa agattgttgg
    15961 gactatcgct attttgaccg caagcattct attaagtgcg tgtggcaatt caggatctgc
    16021 agatgatagt acggggtatg taggaatcgc tatgccgacc aaatcagcag aaagatggat
    16081 cgctgatggg aacaacatga tctcggaatt agaaaaactg ggttataaaa cagatcttca
    16141 atatggagaa gataaagtag aaaatcaagt agcacaaata gaaaatatga ttacaaaagg
    16201 ggtagataca cttgtgattg cttctatcga tggttcggct ttaactgatg tgttagctaa
    16261 agcaaaagaa gcagatatca aggtgatcgc ctatgatcgt ttactgatga attcagaaaa
    16321 tgtcgattat tacgcgactt ttgataattt tggagtaggt gtatcgcaag cagcttatat
    16381 tgaagagcac ttaggattga aagaaggaaa aggaccattc acgattgaat tatttggtgg
    16441 ctcgcctgac gacaacaacg cactaatcaa ttacaacgga gtgatgtctg ttttacaacc
    16501 atatatggac aacggtcagt tggtcgtgcc atctggacag acgagtttca gtcaaatcgc
    16561 tacgcttcgc tgggatggtt ctacggcgca agcaaggatg gataatctgt taagcgcaaa
    16621 ttatactgac caaacattgg atgctgtttt gtcgccatat gatccaataa gtttaggaat
    16681 catttcgtca ttaaaaggcg tggggtatgg gagtgaaagt aaacctttac ctgtgattac
    16741 tggacaagat gcgacagttg ctggcgttaa atcgatcatt gccggggaac aaacacaaac
    16801 gatctttaaa gacacacgta tcttagcaaa aaatacgatt gaaatgatca aagcaatcag
    16861 tgatggagaa gaggttccgg tcaatgatac tgaaacttat gataatgggg tgaaaactgt
    16921 ccctacgtat ttagcaaata cagtatctgt cgataaagat aattatcaag cagagctgat
    16981 cgacaccgat tattataaag aatccgatct gaaaaattaa gaggaaccaa acaaggaact
    17041 aaagagatct aagtcagaga gaggataaga attatggcgg attgtatttt aaaaatggca
    17101 gatatcacca aaaaattttc tggaatccga gcgttagatt gcgtgaatct gatgattgaa
    17161 cgtggggaaa tccatgcatt gtgcggagaa aacggagcgg gaaaatcaac attgatgaat
    17221 gtcttgagcg gcttgtatcc atatggctcc tatgacgggg aaatctttta tgatggaaag
    17281 ctgtgtcaat ttaaagattt gaaagatagt gaagaaaaag gcatcgtcat cattcatcaa
    17341 gaattagcac taagtcctta tctttcaata aaagagaata tttttctcgg aaatgaacaa
    17401 gcagaaagag gcgtgattga ttgggaactg acagaaaaga aaacatatga actgctgaat
    17461 aaagtcggtt tgtctatcga cccgaatctt ctagtatccc agattggcgt agggcaacag
    17521 caactagttg aaatagctaa agcattatca aaatccgtcc gtctgttgat tttggatgag
    17581 cctaccgctg cattgaatga agaagaaagc gcgaatttgc tgagattgat ccgagagttg
    17641 aaagagcaag gtgtaacctc catcattatt tcacataagc tgaatgaaat cgtggatatc
    17701 gcagatcgaa tcaccatttt acgggatgga cagacgattg agacactaga aaatgaaatg
    17761 atcagcgaag agcatattat tcgcggaatg gtgggcagag atttagttga tcgttatcct
    17821 gaaagaaaac cggatattgg agaagtttat tttaaagtag aagactggac ggtctatcac
    17881 cctcttgatc acgagcgaat cataaacgac cacatcaact tgcatttgaa aaaaggagag
    17941 attgtaggaa tagctggact gatgggagcg ggcagaactg aattcgctat gagtctattt
    18001 ggacattctt atggaagcaa gatatccgga cgtatattaa aggaaggggc agagatatca
    18061 gttaagaccg ttccttcagc cattgcaaac ggactcgctt atgtctcgga ggaccgaaaa
    18121 tctttaggac tgaatctgct gatggatatt cgggaaaacg tgacgctgag cagtttggat
    18181 aaaataagtc aaaaaggagt attagataaa gaaaaagaag taactgaggc agaaaatttc
    18241 cggaaaaaga tgcgtataaa aacaaactcc atccatcaaa ttgtcagtag tcttagtggt
    18301 gggaatcaac aaaaagtcgt tttatccaag tggctgatga cacaacccga tattcttttt
    18361 ttggatgagc caacccgtgg gatagatgtt ggggcaaaat atgagatcta cacgattatc
    18421 gaagaaatgg cagcaaatgg caaagctgtc tgtcttatct cttctgaact tcctgaaata
    18481 ttagggatgt gtgatcgaat ctatacgatg aacgaaggac gtattacagg agaagtctcg
    18541 cgagaagatg cagatcagga agtcttgatg aaattaatga cgaaagaaga ggtgggttaa
    18601 gttggaacag gcaaaaaatc ttacggataa aaaagagaaa aaactaaatt tgaaaagtag
    18661 agcagctgat ctttttggtc gctacagcat ggtgatcatt ttagcgggac tacttattgc
    18721 gtttcaattg atgacggatg gtattttctg gcgtccattg aatataacta accttgttct
    18781 gcaaaatagc catatattag tactagctgc agggatgtta ttagttgtgc tcttaggtca
    18841 tgtggatttg tcggtagggt cagtgatggc gtttgttggt gcaattgcgg gcatgatgat
    18901 ggtcaatcgc caaatgaacc cgtggatgac agttatcgca tgtcttgcag taggcgggct
    18961 gatcggcgca tggcaaggtt tttgggtagc ttatgcaggg ataccggcgt ttatcgtgac
    19021 tttagcagga ttgctgatgt ttcgtggact gacacaagtt gttctaggag gccaatctct
    19081 agcacctttt ccagctgttt tccaaaaaat gtctacgggt tatttaccag acttaagcga
    19141 aggaagcagc cttcacttct tgacattgat tttaggaatg atcatcacga tcgtgttagt
    19201 tgttggtcaa tggcgaacaa gaaatcgtcg gaaaaatcat ttatttgaag tggaatctcc
    19261 aacagctttc ttggtaaagg cggtatttac agcagtcgtt gtactaggaa taacttatat
    19321 ctttgcctct tatcaagggt atccgattat tttgatcatt cttggtttga tcgttgcagc
    19381 atatgggttt ttgacaaata aaacagtagc aggtcgacag atttatgcga cgggcggaaa
    19441 tcgaaaagcg gctgaactat cagggatcaa aacaaagcga atcacgttct gggtattcgt
    19501 caacatggga gtcatggcag ctttagctgg attagtttta gctgctcgat tgaatgcggc
    19561 aacacctcaa gcaggtacat cgatggaact ggatgcaatg gcggcagtat attttggtgg
    19621 tgcttcgact tccggcggca ttggaacaat catgggtgcg attgtcggtg gcttggtcat
    19681 gggtgtattg aacaatggca tgtcgatttt aggcgtcggg gtagactggc agcaggcaat
    19741 caaaggattg atcttgcttt tagccgttgt gctagacttg tataataaga aaaaacgaac
    19801 aacgtaggag aacgccggtt attcagaaaa aagactgtga caagacattt gtcacagtct
    19861 ttttttcgct aaaagcgaac attcattgtt tcgtttgtat agactaaaaa taatatatgt
    19921 ttttttacca ttcgtataaa ttctcttttt gctcttgatt tttttgctta attgagttaa
    19981 attacatagg gcgctaaaaa aagccttgat taaattaata atgttcgtgt tttgctggat
    20041 gtgaacatca ggaggataat gtaatgaaag tatttgatta cgaagatatt caattaattc
    20101 ctaataaatg tatcgtaaat agccgttctg aatgtgatac aacggtcaca ttaggtaaac
    20161 atacattcaa aatgccagta gtgcctgcca acatgcagac gattattgat gaaacaatcg
    20221 cagaattctt agcggaaaat ggttatttct atatcatgca tcggtttgat gaagcagctc
    20281 gaattccgtt tatcaaaaaa atgaaaaagc gtggattgat tacctcgatc agtgttggcg
    20341 tcaagaaaga ggaatattcc tttatagaga agttggcaga agaatcgcta aatcctgatt
    20401 atatcactat cgacatcgcg catggacatg ctaactcagt tattgatatg attcagcata
    20461 ttaaaaaata tttgcctgaa acttttgtga ttgccggtaa tgttggtaca ccggaagccg
    20521 tccgtgaatt ggaaaatgcg ggcgcagatg caacaaaagt cgggattggt ccaggaaaag
    20581 tatgtattac aaaaatcaag acaggctttg ggactggagg ctggcaactg gcagctctta
    20641 gatggtgtgc gaaagcagct cgcaagccga tcatcgctga cggtgggatc agaacacatg
    20701 gcgatattgc aaaatctgtc cgttttggtg cgacaatggt catgattggt tcattgtttg
    20761 ccggtcacga ggaatctcct ggcgaaacaa aagtcgaaaa tggaatcgtg tataaagaat
    20821 actttggttc tgcttcagaa ttccaaaaag gcgaaaaacg aaatgttgaa ggaaagaaaa
    20881 tctggatcca acacaaagga tctttgaaag atacactagt ggaaatgcaa caagaccttc
    20941 aatcatctat ttcttatgct ggaggaagag atctggaagc cattcgaaaa gttgactatg
    21001 tcatcgtgaa aaattcgatt tttaatggag acacaatcta aaaaataaat aaaatattag
    21061 gatgttgtga aaaagttgtc ctgcattaag taataagaac agccaaacaa aaatagttct
    21121 ttatatgttt tgtttggctg gaattattat cgcagataca agcttttgaa catcattatt
    21181 cagaataagg gactgtgata agatttttgt cacagtccct tatgttttgc aaaaaaagtt
    21241 tttctttttt ctcgtaaatt cggtaaaatc aaaagaaaaa atagacaata gaaaagagaa
    21301 gcttatgtgg gaaaaagtag agaaaaaaca aggaccgatt tatcgtcaaa ttatggaaca
    21361 gattatgaga gaaatcgaaa acggaaggat caatcctgga agtcaacttg aatcagaacg
    21421 aaaactaggg caactttttg gtgtgaatcg ttccaccgtt gtccatgcat tggaagagct
    21481 tagggagctt ggggtactaa cgagcaaacg tggcagcgga tggtttgtta atcagactga
    21541 atgggggaaa ttcgctgttc caagaatcga ttggcggcaa atgatctctc ctcgttatga
    21601 gcagacggac tggtatgagc aaaaaataag agaagcaaag cagatccaaa aaacatcgtt
    21661 tcttgattta tacgctagcg agatgccaga tgagcttttg ccgaattttg agtttccttc
    21721 ttattctctt gaagaaatct tagcggaaga aaaagaaatg gatcttttag gctatactcc
    21781 gttacgtaac aaaatcaaac ggtatttgga aagtaaattt gaattggcat tttcccccaa
    21841 ccagcttctt gtgactggtg gcggtcagca agcaatcttt ttgattttac aaacgattct
    21901 ttcagctgga gaggggatag ctgtagagtc accttctttt ttttaccgat tacctctttt
    21961 caaagcttca ggaacgcgtt tatttgggat accgatggat gaagaaggga tcgacttaga
    22021 aaaactgaaa gaatcgatcc ataaaaataa attgaaagca gtgcttatca acccgaattt
    22081 tcaaaaccct acaggaaaag tgatgagtca aaaacgacga gaaaagctag tagaattgtg
    22141 tcgacagtat cggcttccaa ttattgaaga cgatgtattt acggatcttt catttctaaa
    22201 ccaagaagaa gtccttccta tacgaacgat tgatccggat aatgtgttat acgtcggttc
    22261 gttatctagg gttttaggaa aaacaacgaa aatcggttgg gtgataggac ctgagacttt
    22321 tatctcgcaa ctagctcaag cgcaagaaat gatggagttt tcgatgaata tccttacaca
    22381 aatcacagtg gctaatgtat ttaatgaagc aattgatttg aaaatgcaag atgtaagaaa
    22441 aaagttgaaa gaaaaaagcc gactgataag cgcttgggga gattcacaat cgtttttcaa
    22501 gcttcattca attttaggtg gttactatgc ctggattact tgggatggga aaaaactaga
    22561 ccccgaacta gcagaaaaag tagtagatat gggtcttgga attgcaccaa gtctactttt
    22621 tgggcaagat ataaatggaa tcagaatcaa ttacagtagg ctggatgaaa accaaatacc
    22681 gttgttttat tcaaagatga aaattctaga aaagtggtta acagaataat ttcacaaagt
    22741 aaatagatta aagaatataa cagcttggat ttaaatttca agctgtttct tttttttcgg
    22801 aaaaagaata cgatataaag aaactaagaa aaaaccatta tttgtacgtc cgttcacgat
    22861 gtgtaaataa aataaatgtt tgcaacaaaa ctgtataaca ccaacgtttt taaatagaaa
    22921 cagatggttg taagtttgat tagaaaatag aaaaaatgca gatgaaatat aaaacgcttt
    22981 ccttattgtg attttattca ctaatgctgt taatatgcgg attggcataa tttcctttca
    23041 tcgtgaaaat ttgtgctttt agcttgtatt tctcataaat ccatgataat ttattaatga
    23101 agacggttaa atagtgatac agttttattc actgtttcaa tcgcagaatg aagagatttt
    23161 cttatagatg agagaggaat gtgtgacaaa tggcaaacaa aaaagcaccg atcgacttcc
    23221 aagcactgct agatgaagtc aatgcagatt tcccagtata tcaagcactt gatcaagatg
    23281 gaaacgtagt taacaaagat ttagtacctg atttatccga tgatgaactc gttgaattaa
    23341 tgactcatat ggtatggtct cgtgtattag accaacgttc aacagcgctg aaccgccaag
    23401 gacgcctagg cttttttgca ccaactgctg gacaagaggc aagtcagctg gctagtgctt
    23461 acgcatttga taaagaagat gttcttcttc caggttatcg ggatgtacct caactagtaa
    23521 aacatggttt acctttatct caagcttttt tatggtctcg aggacatgct gcaggtaatt
    23581 cttatccgga agaattgaaa gctctaccac cacaaattat catcggtgca caatatgtac
    23641 aagcagccgg tgttgcatta ggtttgaaga aacgaaacaa gaaaaatgtc gtcttcactt
    23701 atactggtga tggcggttca tcacaaggtg atttttacga agcaatcaat ttcgctggcg
    23761 cttaccatgc aaatgccgta ttttatatcc aaaacaacgg gtatgcgatc tctacacctc
    23821 gtgaaaaaca aactgcagca aaaacacttg cacaaaaagc tgtcgcagct gggattcctg
    23881 gtattcaagt cgatggaatg gatccgcttg cagtatatac tgtatctaaa atggcacggg
    23941 aatgggcgat cagtggaaat ggaccagtcc taatcgaaac actgacttat cgttatggac
    24001 cacatacact atctggtgac gatccaactc gctaccgtac aaaagacaca gatgacgatt
    24061 ggcagaaaaa agatccattg atccgcttcc gtaaatattt gacggaaaaa ggactttggt
    24121 ctgaagaaaa agaagaacaa gtcatcgaag caactaaaga agaaatcaaa gcagctattg
    24181 ctgaagccga caaagtacca aaacaaaagg tttcagattt cttgaaaaac atgttcgaag
    24241 tatctccaca atcaatcaaa gaacaaattg caatttatga agcgaaggag tcgaaataat
    24301 catggcacaa aaaacaatga tccaagcaat cacagatgcc ttagcacttg aactagaaaa
    24361 tgacgagaat gtcgttgttt ttggtgaaga tgttgggaaa aacggaggag ttttccgggc
    24421 aactgaagga ttgcaggaaa agttcggaga agaccgagta ttcgatactc ctttagctga
    24481 atctggaatc gcaggattga gtttcggtct tgcgttagaa ggatttcgtc cagttcctga
    24541 aatccaattc tttggtttta ttttcgaagc aatggacgaa gtagtagctc aaatggctcg
    24601 tacaagatat cgaatgagcg gcactagaaa tctacccatc acaattcgtt cgccatttgg
    24661 tggcggtgtt catacaccag aattgcattc ggataattta gaaggtttga tcgctcagtc
    24721 tcctggtatt cgagtagtga ttccttccaa cccttatgat gcaaaaggac tattgatttc
    24781 ttctattaga agtaacgatc cggttgtttt cctagaacac atgaaactat atcgctcatt
    24841 ccgagaagaa gtgccggatg aagcttacga agttcctttg gataaagcag cagttactcg
    24901 tgagggaaca gatgtatcta ttatcacata cggagcaatg gtgcgtgaag caatcaaagc
    24961 agcagacaat cttgcaaaag aaaatatatc tgttgaaatc attgatcttc gtacagttgc
    25021 gccattagat gtagaaacga tcattcaatc cgtggaaaaa actggtcgcg tagttgttgt
    25081 ccaagaagca caacgacaag cgggcgttgc cgcgcaagta gtttctgaga tctcagaacg
    25141 cgctatcctt tcattagaag ccccaatcgg acgagtttct gcacctgata cagtcttccc
    25201 atttggacaa gcagaaaatg tctggttgcc aaatgcaaaa gatatcgaag acaaagtaaa
    25261 agaaatcgcg gaattttaag aaaagaaggg acgaaacaaa gtcatggctt atcaatttaa
    25321 attgccggat atcggtgaag gaatcgcaga aggtgaaatc gtcaaatggt tcgtcaaacc
    25381 aggagatacg atcaatgaag atgatacatt actagaagtt caaaatgaca aatcagtaga
    25441 agaaatcccg tcacctgtta caggaacggt taaaaatgtg attgttccag aaggtaccgt
    25501 agcaaatgtt ggggatgtat tagtagaaat cgatgcacca ggacacgaag acaatgaagg
    25561 ggacagtggt gtagccgcag aatcccaaac tccagctaaa ccggctgctg aacccactgt
    25621 tgatacagaa tcagctggat cgtcaagcga aggagtcttc caatttaagt taccggatat
    25681 cggtgaagga atcgcagaag gtgaaatcgt caaatggttc gtcaaaccag gagatacgat
    25741 caatgaagat gatacattac tagaagttca aaatgacaaa tcagtagaag aaattccgtc
    25801 acctgtcaca gggacagtta aaaacgtgat tgttccagaa ggcactgtag caaatgttgg
    25861 ggatgtatta gtagaaatcg atgctcctgg acacaatagt gcaccgtcaa ctagtgcgcc
    25921 atcagctgaa gcaccaaaag aaaaagtgga aacatcaggt tcagcaagtg tcgttgaagc
    25981 tgccgatcca aataaacgtg tattggctat gccatctgtt cgtcaatttg cccgtgaaaa
    26041 agatgtagat atctcacaag taactgcaac tggaaaaggt ggcagagtaa caaaagagga
    26101 catcgaaaac ttcttggcag gtggtccaag cagtgcacca gcgaaatcag aagcgccaga
    26161 agcggcagca ccgaaagaag cagctcctgc agcagaaagc aaaccagctg caccagcaaa
    26221 accattcaag tccaacttag gagacttgga agaacgtgta gcaatgacgc caacacgtaa
    26281 agctatcgct aaagcaatgg tcaacagtaa gcatacagct ccacacgtga cattgcatga
    26341 tgaagtagaa gtgtctaaac tatgggataa tcggaaacga tttaaagaag ttgctgctgc
    26401 aaatggtacg aaacttactt tcttgccata tgtcgtgaaa gctttaacag cgactgttaa
    26461 gaaataccca gtattgaatg catctatcga cgatgcaaac caagaaatcg tttataaaca
    26521 ttactacaat atcggtattg caactgatac ggatcacgga ctatatgtac caaatgtgaa
    26581 agatgcagac cgcaaaggaa tgtttgctat tgcagacgaa atcaatgaga aagcaaaatt
    26641 agctcatgac ggcaaactat ctgcagaaga tatgcgcaat ggaacaatta cgatcagcaa
    26701 tatcggatct gtaggtggcg gttggtttac accagtcatc aactatccag aagtagctat
    26761 tttaggtgta ggaacgattg cacaacaacc aatcgtaaat gctgaaggcg aaatcgttgt
    26821 cggtcgtgtg atgaaactgt cattaagttt cgatcaccgt atcgtagatg gagcaactgc
    26881 gcaacaagca atgaataata tcaaacgtct tttagctgat ccagagctat taatgatgga
    26941 aggatgataa acaaatggta gttggagatt ttgctgtaga attagataca gttgtaattg
    27001 gtgcgggacc tggtggctat gtagctgcca tccgcgctgc tgaaatggga caaaaagtag
    27061 caatcattga aagagagtat attggcggtg tttgtttgaa tgtcggctgt attccttcaa
    27121 aagcactgat agctgcagga caccattatc aagagtcact agattctgaa atgtttggag
    27181 taactagtga aaacgtcaaa ctagattttg caaaaacaca agaatggaaa gaaaacaaag
    27241 ttgtccatac gttgacttct ggtgttggtt tcctattaaa aaaacataag gttgaaacaa
    27301 tcgaaggaga agcgttcttc gtagatgatc acacactacg cgtcattcat ccagattcag
    27361 ctcaaactta ttcgttcaat aacgcaatca tcgcaacagg ttcccgtcca atcgagattc
    27421 caggatttaa attcggagga cgtgtgttag attctactgg cggattagca ttgaaagaag
    27481 tacctaagaa atttgtgatc atcggtggcg gtgtgattgg tgccgaattg ggcggagcgt
    27541 acgcaaactt aggagcagaa gtaacgattc ttgaaggctc accacaaatt ttaccaactt
    27601 acgaaaaaga tctagtaaaa ttagtagaag atgatttcaa gaaaaaaggc gtaacagtcg
    27661 taacgaatgc aatggcgaaa gaagcagtcg ataacggtga cagcgtgaca gtgaaatatg
    27721 cagtggacgg gaaagaagaa tccgttactg ctgattatgt gatggtcact gtaggtcgtc
    27781 gtccgaatac agatgatatg ggcttagaac aagcgggtgt cgaagtagga gaacgaggat
    27841 tgatcacggt ggataaacaa ggacgtacga acgtacctaa catctatgcc atcggtgata
    27901 tcgttccagg ggcggcatta gcacacaaag ccagttatga agcaaaaatc gctgctgaag
    27961 caatctctgg caaaaaagta gctgtagatt ataaagcaat gccggcagta gccttcactg
    28021 atcctgaatt ggcttctgta ggtatgacga tcaaagaagc aaaagatgct ggtatcgaag
    28081 caaaagcata taaattccca ttttcaggga acggtcgggc attatcgctt ggtaaaacag
    28141 aaggattcat ccgtctagtt acgacgatcg aagataacgt gcttattggc gcgcaaatcg
    28201 gtggagtcgg cgcaagtgat atggtctctg aattagcttt agcaatcgaa tcaggaatga
    28261 atgctgaaga tatcgccttg acgattcatc ctcatccatc tcttggcgaa atcgtcatgg
    28321 atgcttcgga attagcatta ggtttaccaa tccatatttg atttgaataa tacaggcgaa
    28381 acgcaactga aaaatcagtt gcgttttgtt ttttagaatt atcaataata ttattatgta
    28441 aacttaaaaa tatgacaact tcttggttta ttaccacttc ctatgctaca ctgaagagga
    28501 atatgaaata ggagagttga agatgcaaga atatcagtat ccacttgatt tagattggac
    28561 aacagacgaa atggtcattg taatgaagat gtgggaagat ctggaacgtg cgaatgaaaa
    28621 agggataaac aatgaagctt ttttgaagac ttatcagcag tttaaaactg ttgtcaaatc
    28681 cattggtgag gaaaaacgat tgggacggga atttgaaaaa gcttccggtt attctttata
    28741 tcgtacagtg cagcaagcaa aaaaaaatcc aaacaaaact ttgagaatga acgggtgaga
    28801 taaatggaac agatgattga tcatatcaag tcctggcttg aagaagctgg tgagaaaatc
    28861 aaatcttctc aacaaacatt aacaatctcg caaaaatcaa atcgaacgga tctagtaaca
    28921 aatgtcgatc gaaacatcca gaattttcta atcgaaaaaa tccattcctt tgatccagag
    28981 gcgaaaatat tggcagaaga agaagggtgt aatcaattat cggatctcga tggaagagtc
    29041 tttatcatcg atcctattga tggcaccttg aattttgtga ttgaagggga aaatttttgt
    29101 attatgctcg ctgtttatga atccggcgtt ggaaagcttg gtttcattta tgacgtgatg
    29161 agaaatgaac ttttttgggg tggaaaagga ataggcgtgt ataaaaatga tgagccattg
    29221 cctagaccag caaataaatc cttatcggaa ggcttacttg gtgtgaatag ttatcttttc
    29281 ggtcataata aatatggaat ccgagaaatt gcggaaagca gcatggggac acgtatgtac
    29341 ggatgcgctg gtcttgaact agtggcaatc ttgaaaggaa accacattgc ttatatttca
    29401 aaccttagcc cttgggatta tgcagcggga aatgtcttat tggaagagtt tggtatggta
    29461 acaagcggtc tttttggtac tcctttgaca tttgaaaaaa gggaatacta tttagcaggg
    29521 acttcttgtg catatgcaga aatcgcagac cttttgaaaa aagaaggctg aaaaaaaggt
    29581 agaaatcatt cacaaaaatg ataaaaacga gcaaaagatt aattttctcg aaaaaatgaa
    29641 aacattttac tatatttcat gaaaaaagtc tgctataatt agcagtcgag taaaatttgc
    29701 cctgtcataa agaaccaata aaaaaggagt tagttacatt gaattataga aacgatatcc
    29761 gtaacgtcgc aattattgcc cacgttgacc acggaaaaac aacattagtt gatgaactat
    29821 taaaacaatc agatacatta gacgcacata cgcaattgca agaacgtgcg atggactcaa
    29881 atgcgctaga aaaagaacgt ggtatcacga ttttagccaa aaacacagct gtagactata
    29941 aaggtattcg cgtaaacatc atggatactc caggacacgc ggacttcggt ggcgaagtag
    30001 aacggatcat gaaaatggtt gacggcgttg tgttggtcgt agatgcgtat gaaggaacaa
    30061 tgcctcaaac acgttttgta ttgaaaaaag cactggaaca acatatcaca ccaatcgttg
    30121 tcgtaaacaa gattgacaaa ccttctgctc gtcctgagca tgtcgtagat gaagttcttg
    30181 aactattcat cgaattagga gcagatgatg atcagttaga ctttcctgtg atttatgcat
    30241 ctgcactaaa tggtacgtca agcttgtcag atgatccagc agatcaagaa ccaaccatgg
    30301 cacctatttt tgatacaatt attgaaaaaa tccctgctcc tgtcgataac agcgacgaac
    30361 cattacaatt ccaagtttca ttacttgact acaacgatta tgtaggtcgt atcggaatcg
    30421 gacgtgtatt tagaggaaca atcaaagtag gagaccaagt agcattaatt aaattagacg
    30481 gaacagttaa gaaattccgt gtaacgaaat tatttggttt ctttggacta aaacgtctag
    30541 agattcaaga agcaaaagct ggagacttga ttgctgtctc aggtatggaa gatatctttg
    30601 tcggtgaaac agttactcct gtggatcatc aagatgcgtt acctatcctt cacattgacg
    30661 aaccaacatt gcaaatgact ttcttagtta ataattcacc atttgccggt cgagaaggta
    30721 aattcgtaac cgctcgtaag attgaagaac gtttgatggc agaacttcaa acagatgtat
    30781 ccttacgagt tgaaccaaca aactctccag atgcatggac agtttctggt cgtggagaat
    30841 tgcatttgtc tatcttgatt gaaaacatgc gccgtgaggg atacgaattg caagtatctc
    30901 gtccagaagt tattgaaaaa gaaatcgacg gtgtaaaatg cgaaccgttt gaacgcgtgc
    30961 aaatcgatac accagaagaa tatatgggta gtgttatcga gtccttgagc ttacgtaaag
    31021 gtgaaatgca agatatggtc catacaggaa acggacaaat ccgtttgaca ttcttaacac
    31081 ctgcccgcgg tttgattggt tattcaactg aattcctttc aatgacacgt ggttacggaa
    31141 tcatgaacca tacttttgac caatatctgc caatgcttcc tggtcaaatt ggcggacgtc
    31201 accaaggggc gcttgtttcg atcgatacag gaaaagcaac gacttattct atcatgagta
    31261 tcgaagaacg cggtacagtc ttcgtagaac caggtacaga agtatatgaa ggaatgatta
    31321 tcggggaaaa cagccgtgat aatgatttga ctgtcaacat cacgaaagca aaacaaatga
    31381 caaacgtacg gtctgctact aaagaccaaa catctgtcat caaaaaacca aaacaattga
    31441 cgttggaaga atctttggaa ttcttgaatg atgatgaata ttgcgaagta acaccagaaa
    31501 gcattcgctt gagaaaacaa attttagaga aaaatgctcg tgaaaaagca tctaaaaaga
    31561 aaaaataatc tgtaaaagga tgaagagaga aaccagcttg ttataaaaaa tagctggttt
    31621 ctttttttgt ccaactcttt tctgtggaga agtcatcttt gtcaatggac ataaattcag
    31681 aaaatacgcc gaaagacgtt taaaagactt ttaaaaaact tatttctaga gagtttttgt
    31741 ttcataataa gataatgttt cacgaaagga gatcaacttg tccttcgaag aaaatcgaag
    31801 taaaatggca agagaaagga gcgggaagat gaacgactta ttaaaacaat actttggcta
    31861 tgatgagttt agacctggtc aaaaagaaat tatccagaaa gtcattgatc aggaaaatgt
    31921 attaggaatc atgcctaccg gaagtgggaa atcaatctgt taccagcttc cagctttact
    31981 attagatggg cttactgtgg ttgtgtcccc tttaatctct ttgatgaagg atcaggtgga
    32041 cgcggcgaat caattaggta tacccgccac ttttatcaat agctcgttag atggttacga
    32101 aacagctaga cgcttccaag aaatcgatag acagcagtac cgtttacttt atatcgctcc
    32161 tgaacgtttt atcatgccgg attttatcca agcaatgaag cgatggaacg tccgtatgat
    32221 cgctattgac gaggcacatt gtatctctca gtgggggcat gatttccgcc caagttattt
    32281 acagatggcg aaccagctag atcaactgcc aaatcgtcct gtaattgttg ctttgacagc
    32341 tacagcgact gtgcaagtag ctgctgacat aaaaagactt ttaaaaattc cagaaaacaa
    32401 ccatattcag actggttttg aacgagaaaa tctacgattt caagtaatta aagatcaaaa
    32461 aaaagaacaa tatctaatcg aatatttaaa gatcaacaaa aatcaatctg gtatcattta
    32521 tgcagcaacg agaaaagaag tagaccgtct ctatcatttg cttaaaaaat tcgatttttc
    32581 cgtgggtaga tatcatggtg gtctaaatga gaatgagcga acagaaatgc aagaagcctt
    32641 tttatacgat cgcttgcagc tgattgtagc gacaaatgca tttggtatgg gtataaataa
    32701 aagcaatgtc cgttttgtta tccattatca aattcctggc tctctagaag catactatca
    32761 ggaagctggt cgggcgggaa gagatggtct ttccagtgaa gccatcctgc ttttctctcc
    32821 tcaagatatc caagtccaaa agttttttat ccagcagtcc caaagagaag aagggcaaaa
    32881 gcaaaaagaa tatgaaaaat taaaagcgat gacagaatac gtgtatattg agtcctgtct
    32941 tcagcaatac atcctaaact attttggcga gacaagcagt ccgtgcaacc gttgtggtaa
    33001 ctgtttagat gacagagaaa tagtagaagt aacgacagaa gcacagatgg ttctttcttg
    33061 cttaaaacgt atgggtgaga actatggtaa acaaatgctg atgaaagtct tagcaggatc
    33121 gaaagaacaa aagctgcaag ctttaggttt tggacattta tctacctatg gactgatgaa
    33181 aaaccaatcg caaaaagaaa cgatgcagtt aattgaatat ttgatatcga atggctatct
    33241 tctgaccatc aatggtgagt atcctgtttt gaaagtaaca gaaagaggga tacaagtatt
    33301 gaaaggacaa gaatctgttt atcgaaagga acccaagaaa gtccagcagc tatcggatga
    33361 agaaaccgat acactatttg aagtgctgcg ggaactgcga acggatctag catcagaagc
    33421 aggcgttccc ccgtatgtcg tattttcaga ttccacattg aaagaaatga gccgaatacg
    33481 tccatcaagc agattggaga tgcttcagat aaaaggagtt ggccaaagca agcttgataa
    33541 atatggcgaa gcctttttat ctcggataaa aaatgcggac tctaacgttc tggataagta
    33601 gaaagaacga atacgtaaaa atgaataaaa acaactggtt aaatctgttt tttttgctcc
    33661 ttatgaaaat caaatatgtt ataattaagg aattagaatg ggaggtaagc acagatggat
    33721 tctttgtcga aaaaacttgc gatccaagta ttaaatgatg aagcagaacg gatcaaacga
    33781 ttaatccgaa atcaaaacaa cagtctctgt atctcacaat gcaaagcctt cgaagaagtg
    33841 gtagatacac agatgtacgg cttttcccgg caagtcggtt ttgctgtccg tataggagta
    33901 atcggaaaga acgagggcca gctgatgtta agtgagctag aacgagaatt gaatcagtta
    33961 tatagtgagg tttaccaaga aatttatgat aaaaaagaag ttggcaagga ggaatagact
    34021 tgcctaagaa agtgaaaaaa aggcatctat tagactatag catcttgatt ccctatttaa
    34081 ttttatgtgt attgggattg atcatggtat atagttctac gtcgtatcta ttattagaaa
    34141 acggacaaaa tccgtccgca tcagtcatta accagagtat tttttgggtg cttagcttga
    34201 ttgctatcgc actactttat aaaatgaaaa cagatgtttt gaaaaaccag cgtttgatca
    34261 tggcagccat cgcagttttg acgattttgc tgctgattgt tgtgtttttt ggtaaggaga
    34321 tcaatggggc gaaaggttgg ctgcaaattg caggattctc gatccaacct gccgaatacc
    34381 taaaaatcat ttctatctgg tacctgtctc tgactttatc taaaagacaa aacagtgtac
    34441 aaaaaaattt tcttgggact gtgaaacgtc cacttgcaat ggttatcgga cttactgcat
    34501 tagtcgctat tcttcccgat tttggtaatg cggcagttat ctttttgatt attttggttc
    34561 tgcttttagc cagcggagtc aattatgtgt atactttgat tgtaggagta ggaggctttt
    34621 gtctaagtac ttttacgatc tggctgatca atatcacaaa cggaaagatc ttacccggac
    34681 gtttacagta tatctataat cgatttgcta tttaccaaaa tcctttctca gacgaattaa
    34741 ataaagggca tcaactagtc aacggctact atgccatgtt caacggagga ctatttggca
    34801 ggggattagg taacagtatc cagaaaaaag gatttttaca agaagcacaa accgatttta
    34861 tttatgcaat cgtagtggaa gaactgggag tgattatggg gattttgata ttagcgttgc
    34921 tattctttat gattgtgcgc attgtccttg taggtatccg ttcaaaagat ccgttcaatt
    34981 cgcttttgtg cattgggatc ggcgcaatgt ttttgatcca agtatttgtc aatcttggag
    35041 gaattactgg cgttattcct ttgacaggga tcacgttccc atttttaagt caaggaggct
    35101 ccagtttgct gatgctttcg atttgtgtcg gctttgtcct caacatcagt gcggatgaaa
    35161 aacgcaagag cttaggttta taataaaaaa agatgtagga gtggttcgat gaaaaaagtt
    35221 ttagttgcta acaggggtga aatcgctgta cgtgtatttc gtgcttgtac agaattagga
    35281 attaaaactg ttggaatcta tgcagaagaa gatgaatatt ccgtacaccg tttcaaagcg
    35341 gatgaagcat atttagtagg acaaggaaaa aaaccgattg atgcgtatct agatattgaa
    35401 gggatcattt ctatcgcgaa agaatgcgga gctgacgcta ttcatccggg ttacggtctt
    35461 ttgtcggaaa acttgaattt tgcgcaacgg tgcgaagaag aaggcatcat ttttgttgga
    35521 cccaaactgc atcatttaga tatttttgga gataaaatca aagcaaaagc tgcagctatc
    35581 gaagcgggga ttgcttcgat tccaggcaca gatggaccaa tcgcttctat agacgatgcc
    35641 ttggagtttg cgaaacaata cggttatccg attatgatca aggcagctct aggcggcggc
    35701 ggacgcggca tgcgtgtagc tcacgatgaa aagagtgcaa gagagggcta cgaacgtgcg
    35761 aaaagcgaag caaaagcagc ctttggtagt gatgaagtgt atgttgaaaa atacatcgct
    35821 aatcctaagc atatcgaagt acagattctc ggggatacgc atggaaacgt catccatctt
    35881 tttgaaagag attgttctgt tcagcgccgt caccaaaaag tagtagaagt agcgccttgt
    35941 gtctctatga atgaacagca acgtcaaaaa atctgccagg cagctgtcca attgatgaaa
    36001 tatgtcgggt atgtcaacgc aggtacagta gaatttcttg ttgaaggaga tgatttttac
    36061 tttatcgaag tcaaccctcg cgtccaagtg gaacatacga tcacagaaat gattacagat
    36121 attgatatcg taacaacgca actgttgatt gctcaaggtt tggatctgca taaagaaatc
    36181 ggattgcctc agcaagaagg aattaaatta aatggttcag ccattcaatg ccgaatcact
    36241 acagaagacc cgttgaataa ctttttacca gacacaggga aaatcgatac gtatcgttca
    36301 cctggtggtt ttggcgtacg tctggatgtc ggaaatgctt acgcgggcta tgtagtgaca
    36361 ccgtattttg attcattgct agtaaaagta tgtacgcatg gtgctacctt tgaaacagcc
    36421 attcagaaaa tggaacgttg cttgaaagaa tttagaatcc gtggagtgaa aacaaacatt
    36481 ccatttatgc tcaatgtcat cacacatcct gaattccaat caggaaatgc aaaaacaacc
    36541 ttcattgata gtacagcaac attatttgaa tttcctcgac tacgagaccg cggaaacaaa
    36601 acgatgaaat atatcggtga aattactgtg aatggttttc caggtatcga aagtggcgaa
    36661 aaaccatttt atgaagaacc acgtatgcct aaagatctaa tcacaagaac tgattacgta
    36721 acggctaaga atgtattaga tgcgaagggt gccgatgctt tggtcgaatg gatcaaagga
    36781 caggaaaatt tgttgttgac ggatacgaca tttagagacg cccatcaaag tctgctagca
    36841 actcgtgttc ggacaaagga tttcaagcag attgcacgtt tgactggaga aggtcttcct
    36901 gaactctttt caagtgagat gtggggggga gcaacatttg atgtggctta ccgctttttg
    36961 aatgaagatc cttggcaacg gttgagaaaa attcgttctt taatgcctaa tacgttgctg
    37021 caaatgcttt tcagaggttc aaatgccgtt gggtattcta attatcctga caatgtattg
    37081 gtggaatttg tcaaagaagc agcagcgcaa ggaatcgatg tatttcgaat ctttgatagt
    37141 ttgaactgga ctccacaaat ggaaaaaagt atccaagctg tacgggatac tggtaaaatc
    37201 gcagaggcag ctatttgcta tactggcgat atcaacgatc caagtcgggc aaaatacaat
    37261 gttcagtatt ataaagacat ggcaaaggaa ctagaacaac tgggtgcgca tatcattgca
    37321 atcaaagata tggctgggct gcttaaaccg caggcagctt atcgtttgat tagtgaattg
    37381 aaagaaacga cagatctgcc aatccatctg catactcatg ataccagcgg gaatggaatc
    37441 atcacttatt ctgctgcttc aaaagcagga gtagacatcg ttgacgtggc aatgagtgca
    37501 atgagtggga atactagtca gcctagcatg agcagcttgt attatgcact ggtcaatggt
    37561 ccacggctgc cagaaatcac gattgaaaat gcacagaaat taaaccatta ttgggaagat
    37621 gtgcgcatgt attacaaacc gtttgaaaac ggcttgaatg ctcctgaaac agaagtatat
    37681 atgcatgaaa tgccaggagg acagtattcg aatctacagc aacaggcaaa agctgtcgga
    37741 ttaggtcatc gctgggatga aatcaaacaa atgtatcata ctgtcaactt gatgtttggc
    37801 gatatcgtta aagtaactcc atcatctaaa gtagttggag atatggcact atttatggtc
    37861 caaaatgatc ttacagaaga agatatttat gaaaaaggcg aaacactaag tttccctgaa
    37921 tctgttgtga ctttcttcca aggagaacta gggcaacccg ttggcggatt tcctaaaaaa
    37981 ttgcaaaaga ttattcttaa aggcagacct gcattgaatg aacgtccagg tcttttggct
    38041 gaatcagtcg acttcaacga agtaaaaaaa gaattggcag aaaaaattgg ctatgaacca
    38101 aaacaagacg aagtattaag ctatttgatg tatccgcaag tctttttgga ctatcaaaaa
    38161 gcatataatc aatttggcga tgttacatta ctggacacac caacattttt ccaaggtata
    38221 cgtttgggtg aaacaatcaa tgtacaaatc gaacgcggaa aaacgcttat cattcgttta
    38281 gatgaaatcg gagaacccga tatcgaaggg aatcgcgtac tgttcttcaa tttgaatgga
    38341 caaagacgtg aaatcactat caaagataat tcgatcatca gtacggtcca aacccgattg
    38401 aaagctgaac cgacaaaccg agaacaaatt ggagcaacga tgtctggttc tgttttggat
    38461 gtattagtga aaaaaggcga taacgttaaa aaaggtgata cgttgatgat tactgaagcg
    38521 atgaaaatgg aaacggccat cgaagcacgt tttgacggag aagtcgcaca tgtatatgtg
    38581 tcttcaggag acactatttc gtctggtgac cttttgattg aagtgactga gaaataataa
    38641 gcgaaaggtg gggaaacatg aaaagactgg gtttgttttg gctgattctg ctattagtta
    38701 ttgtggcatt ctacatggaa ccagtcttgt ttccgccaaa acttgaagtg aaggaaaact
    38761 ctgatcaaca aatatcaaac caaatgacaa cgtggaaata ccaggagctt aaagcaactg
    38821 gtttttctac ttatatcgga aaaccgatcg atcagctgga aaaggatttt ggccaagcct
    38881 atgatcgatt aactagcgga tttggttttg aaacaagata ttatgaaaat aaagctagtc
    38941 atctcacatt tgaagcgaac atccaagaca gcagagtcac aactgttaaa gtactgcaaa
    39001 cagggcctgc tgatatcgcg ccgttttatt tagggatgac gatgcaagat cttacgaaaa
    39061 tcactactat ctatactaat ttcacttttg agtaccatga tacagaagtt ggaatcgaac
    39121 taatggaaga ggatatgaat tatcgtccgc taattgcttt tgataacgac acttttgcta
    39181 ttttattttt tgatcaaaca acaaacggcc tattttcagt tgtgtattta gataaagata
    39241 gtcttttgaa actattgcca tatcaagtgt ttggtgaagg ccttccacat tatcaagcag
    39301 ataaatcggc tgattgggaa tcaatcaatc gcgttaaaga acgaaaaagt acgactttat
    39361 taaatatgct tagaagagaa gacgaacttc ccatatacaa tcaaacactt gcctttcaaa
    39421 accacagtca gctgctacta catgattttt tgacaaggcc tgaggaaata ttgtcagagg
    39481 aacgtatggc tgattggcaa aaatcgctag gcagtgctca aacgacagtg aaattctcac
    39541 tgacgaatga tgaattggag aagttaatca gtaaacaaaa attagaaaat actagtgggc
    39601 tatttattca tccggcgatt gacgctactt tttcgttttt atattggtac agcgatcctt
    39661 acaaccatga ccgtttcatg gacccgggaa cggattctgt aggcattgct ttttctaaag
    39721 aaaatatgtt agtcttattg caagaagaaa acggagaaag tagtgatagc agtgatcgtt
    39781 aacgaagagc tgtttcaact ggaggatcaa tgcctccaag tcgctgaaat gataaaaaag
    39841 agtaaaacga tggcttctta tcttgaacat aaacagtcaa tggaagaatc tgcaaaagtt
    39901 tgccagttga aagaagcgtt cttgtctaag aaagaagcat ttgaaaatgt agcggcatat
    39961 ggtgagtatg cacctgatta tcgtgaaaaa aaacgagttg ttcgaaaagc aaaaagacag
    40021 cttgatatgg atgagcatgt tgcagctttc cgatatagtg aaacacagct gcaaagtctg
    40081 ttggatcaaa ttgtccaagt acttgccgaa gaagtttcta atgaaatcaa agtagatgca
    40141 ggtagcccgt tttttgaaac gaaaaaacac tttggatgcg gaggtaattg tcatgcaatt
    40201 agacaatagt gaatttgaaa taacaaaacg acgcgggctg atcgtatggg tctacagctt
    40261 aaaacaatta aagaatctga aaaagttcgg cttgttgcat tatgtctcaa gaagaatgaa
    40321 atatgtcgtg ctttacttaa atgaagagac attggaagaa acagaagaac gaatccgtaa
    40381 attacatttt gttcgaaacg tagaacgttc ttaccgaccg gatatcgaaa tgaattttgc
    40441 cgaaaaaata ggaaacaaaa cagtattaaa agacgaaggt ttcgaaattg aagagctaaa
    40501 tacagaaatc cgattagctg aacatcttta aaaatctctt atgagcagtt tttaaataaa
    40561 aaataacttg gataacgatc gatttttgat cattgtccaa gtattttttt ggtatactag
    40621 taccagtacc atttctaagg agtgagtaga tgcggataat atcaggaaat tatggcgggc
    40681 gccgtttaaa aagtttagca ggggcgaaca ccagaccaac tacagataaa gtaaaagaat
    40741 ccatattcaa tatgattgga ccatattttg atggagagac agtattggat ctgtttgcag
    40801 gaagcggtgg tttagctatc gaagcagtat ctagaggctg cagtcatgcg gtatgcgtag
    40861 acaaaaatta ccaagcgcta aaaatcatca aagaaaacat cgaaattacg aaagaaccag
    40921 agaaatttac cacaataaaa cttgatgcgg acaaagcaat cgaacggcta gcagcggaga
    40981 aacaacaatt cgactacttg tttcttgatc cgccatatgc aaaacaaaaa ataatcgatc
    41041 aaatccataa aatggaagaa ctgggacttt tttctaaaac cgcagttatc gtatgcgaaa
    41101 cggataaaac agtcgaattg ccagaaactg tggctggatt ccatcaaata agaaaacaga
    41161 tttatggcat aacggccgta actatctata gaaaagaggg aaatcaatga aacgtgcatt
    41221 attcccagga agttttgatc cttttacaaa aggtcactta gatacggtgg aacgtgcggc
    41281 taaattattc gatgaggtag taattggtgt ttttatcaat acttcaaaga aaagtttgtt
    41341 tcccccagaa gaaagaatga cattgatcac aaaagctgtt tctcatcttc ctaatgtaaa
    41401 agtcatgcat caagaaaatc aattgacggt agaaacagca aaagagattg gtgcagatgc
    41461 gttgatcaga ggcatacgaa gtatcaaaga ctttgaatat gaacgagaga ttgctcagat
    41521 gaatcatcat ctatatgatg aattggaaac tgtcttctta ttagcaaaac cagaatatag
    41581 tcacgtgagt tccagtattt taaaagaagt actgcacttt ggcggagatg tgagcagcta
    41641 cttaccgcca gtgatcaacg aagctttagc tggaaagcgt gaatcaaatg aaagctaaac
    41701 aatggttgaa aacagtactg attatcatat ttggaatgtt gatcattgga tttgtagtag
    41761 ttcccttgcc gtattatgta gaatcaccgg gagccacgat cgatctgaaa gaactaatta
    41821 cggtcaatga taaggaagat aaaggttctg ggtctttctc tcttacctct gttggtatca
    41881 ggcaagctac aggtttctta gctttaaaag ctaaaatgac cccttttgaa gaattgatca
    41941 gtgaagaaga attgactggt ggggcgacaa atgaagaata taatcaaatg caacagtatt
    42001 atatggattc ttcccaaaat gcagcaatcg aacaagcgtt aaaacttgca gacaagcctt
    42061 ataaaatgga attcaaaggc gtatatgtca tgagtgtaga gaaaacatcg aatttctttg
    42121 gcaagctttc agttggagat acagtaacaa aagtagacgg gaaaagcttt caaagtacag
    42181 aagcatttat ggattatgta aaaagccaaa aagttggaca aacaatcgaa atcagttatt
    42241 tgcataatgg tgaagaaaaa acagcttctg gtgatctgat cgagttacca acagataaaa
    42301 aggcaggaat aggtatcacg cttactgacc ataccgagat cgaatcagat acagatgttc
    42361 gtattaattc tggctctatc ggtgggccat cagcgggatt gatgttcact ttagaaatct
    42421 atgaacaagt cactggtaaa aacttgcgac atggtaagca aattgcagga actggaacga
    42481 tcaacagtga aggagaagtt ggccgaatcg gaggaatcga taaaaaagtg gcaagtgcgg
    42541 ataaagctgg cgtagagatt ttctttgcac cagatgatac gatttcagac gatgttaaga
    42601 aagaatatcc tgaaataaaa accaactacg aagaagcaaa agctgccgct aaaaaattga
    42661 attcctcaat gaaaatcgta ccagtaaaaa cagtacaaga agcgcttgac tatctagaga
    42721 aaatgaaatg aaactaaata attaaagagg ggttgtgaca cgagttctgt cacaacccct
    42781 ctttttcgtc aaaaatcttc acgataaaaa taaaaacaaa agaaaaaatt gacgtaatta
    42841 gatgttgaag ggacgatttt gccagagctc actgtttctt caattttaaa tgaaaggagt
    42901 aatgaatgat ttttactggt cattctttga aaaaagaaca atgatagaaa aaatcagcaa
    42961 gaaaatacta gcttcaccca aattgatcat ttggatgtct atgatagttc ttatcttttg
    43021 tatcgcaagt ggttttttat tatcaaattt gctattttat agagaaacag aagagactgc
    43081 tgcaaaagaa atactttctt cgtttagttc cagtggagaa gatccatcag ctgaaggaaa
    43141 cgatgtagaa gaggatgctc gtccaaaggt catgtacacc gatatcaaag gaagtgtgaa
    43201 ggagcctggt atctattcct tttcttcaga agaacgggtc tatgatgtac taaagcgtgc
    43261 aggtggatta ttagaagaag cggatagcga tcgaatcaac ttttctgcga agatagaaga
    43321 tcagcaagtc ctatatatcc ctgctgtagg tgaagagctg ccagaacatc ttgatcagtt
    43381 ggcatcctcg gaaggaaaac agcctacagc agattccgag cctcccaaaa taaatatcaa
    43441 tacagccact ccatcagaac ttcagcagat tcctgggatc ggatcagtca aagcgcaaga
    43501 aatcattcga tttagagaag aaaacggatc cttccagaaa gtggaagacc tgcaggagat
    43561 ttctggtatc ggtgaaaaga cagttgaaaa actaaaaaac ttcgttacaa ttaaatagaa
    43621 tgattcaaaa tcgactggca cgagcaataa atggacgcta atggatacta agaataatcc
    43681 aattctaaga gcttggcagg acgaggaggt agtatttttg gataggaaat agcttgattg
    43741 ttcgcaaaga aacattaaaa aaaccaatgc tgaatgtcta tctccaaaat aaaatatctg
    43801 ggattcatat tatgaatacg gctgtcagcg gcaataacag tcaagctcta cgagaacgct
    43861 ttgccaaaga tgtacttagc tatacagcag ataaagtatt tattctcatt gggacgaatg
    43921 atctagcaga gcataaacaa ctttctaaag aaacgtacca aaaaatctgc agtggatgat
    43981 agttcagctg aaaaaagact atagttctga tcatgctttc tttatcacgc caccagcagt
    44041 cgatgagaca aaacaaaaat atcggaataa caagttgatc aaagaatata tctctgttct
    44101 cacagaagta gcaaaacgcc atgattgtga agtatcgatt tttatacgga atttcttgaa
    44161 caaatggatt cgaagaattt ttataatggg ctaatggatg acggacttca tttcggcagt
    44221 gaagggtagg atcttttgtc agacaggatg attcgttata tttaaagaat attttgttta
    44281 agcaatgagt actttttaat caacttgaaa aaacggagga atataaaaca tgttagaaaa
    44341 atcttcaaaa gcatgcgata cgaataaaga aaaagttact gataagtcac ataatgaacg
    44401 cattccttgg gaccagtact ttatggctca ggcagtactg ttatctttac gtagtacgtg
    44461 tacacgttta gaagtaggag cgacactcgt tcgagaaaaa aggatcattg ctggcggcta
    44521 taatggagca gtcagtggag atgtccactg catagatgaa gggtgttacg tcgtggatgg
    44581 acactgcttg cgaacgatcc atgcagagat gaatgcactc ctacagtgtg caaaacttgg
    44641 tatcccgacg gaaggttcag aaatctatgt cacacatttt ccatgtttgg cctgtacaaa
    44701 ggctttgttg caagctggtg tgaaagggat caattatctc catgactatc gaaatgatcc
    44761 ttatgcccaa gcgttgatcg aacaattagg tgtatctgta caccaagttg ctttagactc
    44821 acagcatttt tcaaaacttc aaaatgaatt aacaggaaaa catgtctagc gttttagaaa
    44881 ggcatgttcg aaatcggttg atttttccgg caattacgtc gatgattatc tgtttgttgt
    44941 attacactga caattggggt tatcgtttgc ttcttgccgg aattttttta tggttttcgc
    45001 taaaagaagg acgttttatc gcgtacttgt gtattttgtg tagcataacg gtatccattt
    45061 cttataggct acaagagtat tcatttttag aggaaaaaac aacatctgcg caaattactg
    45121 tatttacaga tacgattcgt gcaaatggta attttttgac aatggaagga aggttatcag
    45181 agaataaaca aaagctccag ctttcttatc aagtaaacac agaagcagaa ttgaaaacat
    45241 ggcttaactt ccgaggaaag ggaatagaat tgactgctga aggcgagttc atctctccag
    45301 caaaaaagcg gaatctttat aattttgacc aagaagctta cttcaggaac catagaattt
    45361 ctggacggtt ccaaattcaa aagattgaac agatcaccat gcaaaatcac tggcgaaact
    45421 ggcttcagag aaaaagagga tcttttatct cctttgtcca atctgaattt ccgcaaaaaa
    45481 caagtgtata tattaattct ttattatggg gctataaaga tgcaggattc agagaaccag
    45541 aagaaatttt tagggagagt ggtttgctgc atctgttcag tctatcgggt ctacacatcc
    45601 aattttattt agggtggctg tattatttat ttcgacgtat cggatggcca ctacacgtca
    45661 gtattattcc gttgagtcta ctaactatct gttatgtttc tattgcagga agttctattt
    45721 ctgttttgcg tgctagtttg ctgtttttat tgcgattgct agtcaaattg ctgaataagc
    45781 attattccac catggactta tttgcagttg tcttttggct tcttttacta tttgatcctc
    45841 gatttctttt tcatgcgggt ggacagctta gttttttcat gagttttttg tttatcctga
    45901 tttcttttga tggagattgg ttgaaaaaaa cggcctctca cgtcctgttt actatgatca
    45961 cgatgccgtt gattgtatgg catttttatg agtggcctat tttaggtagt ctttttacat
    46021 tactgattgc accggtattc aagtttcttt ttcttcctgg tgtgttattt ctcacactct
    46081 tcaatcgctg gctgccggaa actttacttt tgctgataga agagggatta atcttatttg
    46141 agcgaataat cgatttttct tcagggtcgt ctctcattat aggacgtttg ccccttgtgc
    46201 tatctgggat gtatattttc ggattactga tttgtatgga taggttgaaa gaacaacctg
    46261 tgaaacactt gttttatgtt ggtctttttt cttttatttt tttgactttg ccatttttac
    46321 gcttttcttc aacgatcgct tttatcgatg tcggtcaagg agacagtatc tttttgcaaa
    46381 gtccttttcg aaaagaaacg attttgatcg atactggagg tcagttacac tttgaaagag
    46441 aaaagtgggc gcaaggtatg atacgtcctc ctgctgaaag aaatatcgtt ccatatctaa
    46501 aaggtcaggg gataagtgta atcgataaac tcatcttgac acacgacgat acggatcatg
    46561 tgggagaatt acccacgtta tctcagcatt ttactatcaa aaaaatttat atcggttggg
    46621 gcgctggacg aaatgagcca ttacaaaggc atttatctga agctcaaaaa aatggtactg
    46681 agatcattga aatcaaacat ggggacagga tcagcggtta ttatgatctt tatgtattga
    46741 ctccttttga aaagggagag ggaagaaatg aagattcgat tggtctatgg atggaatata
    46801 acaataggcg ctttttattt ttaggagatt tgaatcaagc aatggaaaaa caattgcttt
    46861 tgatttatcc taatctgaaa gccgatgtag tcaaattagg tcatcacggt agccgtacgt
    46921 caagtaacac agattttctt tcccatattg aagcaaaaca tgggattata tcctgcggcg
    46981 taaataatcg ttatggtcac ccgcacagcg aagtattgga gacactagaa gaaaatcaga
    47041 taaaaacatg gcgaacggat caacaaggaa tgatcaccta tcgctggcat ccagtttttc
    47101 atcctcatgg gctagtggaa acaatgatag actagtaatt ctcatctgct catggtagga
    47161 taatttttga aagtggtgag taatttgagt atacaagcag agttacaaaa aatacgtaaa
    47221 gaaaaacttg catcttgtta cttgatttta ggaacagaaa agtttctcca agatcaagtt
    47281 cggacagaaa ttgtcaaaaa aatccaatta gagggcgaag atgacttaaa ttttttaagt
    47341 tttgacatgg aaaatgcaag tttagatgag gtagtagctg aagcagagac gttgccgttt
    47401 ttcggagatc aacggttagt attcgtagag aatccttatt ttttaacagg tgaaaaagta
    47461 aacaatggaa tcgaacaaaa tactgattta ttgacggctt atttgaaaga accattagaa
    47521 tcgactgtat tagtattctt tgcgccatat gaaaagttgg atgaacggaa aaaagtaacg
    47581 aaacaattga aaaaaacagc aattatcatc aatgtccaac agttgaatga aaaagaagta
    47641 cgtcaatatc tgatgaatac cttagagaat tcctcaatca aaatggaacg aagtgcaatt
    47701 gatttattct tgaggttgac cgacttagat ttatccaaaa tgatgagaga gttacagaaa
    47761 ttggtccttt atggacaaaa tcaacagcaa atcacggtaa gagaagtaga agaattagtt
    47821 cctaaaacgt tagaacataa tatttttgac atgacccaat atattttaaa agggaagacg
    47881 gaacaagctt tgcgtttata cgaagattta gttatgcaag gggaagaaac aatcaaaatt
    47941 aatgcgattt tgctttctca attacgttta tttttacaga caaaatttct tgtaaagatt
    48001 ggttatcagc aggcaaatat tgctgaaaca ttgaaaatcc atccatatcg tgtaaaactt
    48061 gccatgcagg aagtaaaaaa attcgatgaa catttgctgg tacagttgtt tgatcaatta
    48121 gttgagatgg attatcaaat caaaacggga caaatcgaaa aagaattaag ttttcaacta
    48181 ttcgttttgc gcacagggca gttgttaaga aatcgataag aagaaagacg agggcaaagc
    48241 atgcgggaag gtcgtttgtt tttgtccttt ttttgacaaa taaaaaaatc ggccgaagcc
    48301 gattctttta tttagcgatt tttttgctta aacgagattt gtcgcggtta gctttgtttt
    48361 tgtggattag accttttgat tcagccatat caatagcttt aacagcttct ttatataacg
    48421 catctacgtt gtcagcacct gaagctacag catcttcaaa ttttttgatt gcagtacgca
    48481 tagcgcttgt ttgagatgaa tttttcacat tagcgttttc gcttgtacga acacgtttga
    48541 ttgcagattc aatgtttggc attagtttca cctccgataa cttaagttct tacgcgtgtt
    48601 accgcataat tcaacattca ttattatacc taaaggcagc gtgctttgca atatgaacat
    48661 gacaagtttt tttccttaac agtcttttat taggattttt tttcagtaaa aacggatagt
    48721 acaagctgaa aaaaatcaaa aagacgctat tgtgggaggc aatagcgtca aaccaaacta
    48781 gatttttatt tatctattta aacaaagttt gaatgggaat gtttggtact aagttattat
    48841 ataacaaaga gaaccgtttg caaagttttt tttgaaaagg gatacgattc cctgttgttc
    48901 tttgtttttc tattaagaaa ctgcagttat tttataacca gtatcctttg atcagtaaag
    48961 gctattctgg tgtaccgatt attttaatgt attcatcgat attttttatc aacatttttt
    49021 ttatgaccaa ctggttccga cggttccatt tgccacttac ttccagcaca tatttattta
    49081 ggggcaaacg taagatatat tttgccaaat agcgatcttt aagcaggcca ttgatcacac
    49141 ggtcctcaag ctgcaagttg atcccagtca gcatcattgg ctgttcttgt aaaacgtacg
    49201 tatcaattaa aagaccttca aaagtttcca caagcgtctt cctttctttt caaagtaacc
    49261 tgaaaacagg cactaattct tccattttga tattcgttga agtggatgcg atcattcttt
    49321 cttttgtcca ctgtaatcta gtataacaaa aaagagcaga agagccaaaa agaaaaaaga
    49381 gcatcgaatt ctataggaag tctcattggc acggcctaaa ttattacaaa acattgatta
    49441 aaaaaagccc ctgcatcgca ggagcatccc ctatatcgtt ttaataggtc aatcaaaaag
    49501 cagtatcaaa cacattttgt atcaaatcag gaaataatag ataagctaag cccagaatca
    49561 aaagtaccgc tagaagaaac cagccgattg ttcctatgaa atactttaat ttttcgaaca
    49621 ttgtatcacc tcgttgtcag tatatcacgg atcagtaaat tgggggtgtt aagaatctcc
    49681 taaaatagac aaaatttgtt ctattttcct aaagcaaata cttgtaagat acagtaagtg
    49741 ataccagaag caatgaccgg tccggcggcg attcctctta agaagacaac acctaaaatc
    49801 gtgccgaaaa caagtgcaac agtgatttgt ggatcagaag caatcaatcc tacacccttg
    49861 gaagataaga cagccactag cccaccgcaa aaaacagcta cccagccaat aggggattta
    49921 aaggcttcta atagatcacg tagaccgatt tctcctgttg cgatagggat tagaatagcc
    49981 gctgtgatga tcgtcacccc aatattgatt cctttgcctt gaagccaagg gaaaaaggaa
    50041 cgggaggctg gtatgagctt cataatcaaa acaaaagctg ccgctatcat cagactttga
    50101 tttttagcta aaattgctac aagtaaaata attcctaaaa aaagccaact ttccatgaaa
    50161 tatattcctc actttcttcg aaacaatcat aacatgtttt tagaaagtta caaaagtagg
    50221 aattcatcct tcaagaccta ttcataagct gtttttttca taaaataaac aaaatcataa
    50281 aaaaacactt tgcttatttg acagaagaca gccgcgcttt gtatagtgga caagacttac
    50341 atttttttga agaatcagaa agatcaggta aaggtcttag taaacgcaag ccattcagaa
    50401 taaccaatat tgtgcttcct tcgtgtccta caactccaag aggtagattt aaaaattgca
    50461 ggaagttaga agcaatcaac aagagaataa caagaatcga aaaaataata ttttgtttga
    50521 tgatccgccg catttttaaa gatagccgat gactgtagac aaatttattt aagtcgcttt
    50581 tcattaagac gatgtctgcg atatccatag cggcatctgt cccttgcccc atagcgacac
    50641 ctaaagaagc ggctgctaaa gcaggcgcat cattgactcc atcaccaacc atcgcattta
    50701 cttggtaaag ttcattctct tttttgacta gttgtgtttt ttcttccgga gtacagcttg
    50761 catagtaatg ttcaatccct acttgttcag caatggcctt tgctgtgcca gaatggtcgc
    50821 cggttatcat acttgtatgg atgttttggg atttaaagta ggaaattact tgttgggtat
    50881 ttgcttttgg aatatctagc agacctaaaa cagcaattaa ctgctgatct ttagaaagat
    50941 aaatcacggt tttgccttga ttttccaaac gttcgatcat ttccgcaatc tctttgctga
    51001 ttatcatcgt ctcaggatca tatgcgtgct tgccaacttt ccattgcgca cctaaatagg
    51061 tagtttgtaa tccaaaacca gtaatttctt ctattggtaa gtattggatt tcttcaggta
    51121 ttgtgcaatc gaaatattgc aaaatcgcat gagcaagtgg atgcgtcgtt ttttgttcca
    51181 tagcaacaag aagttgttgt gccaaatcct tttcatcaaa gaaaaagctg tcagttacaa
    51241 ctggttttcc tttagtgagc gttcctgttt tatcaaaggc aatcgctttt aattcagaaa
    51301 gcttttctaa atggataccg cctttgatta aaatcccatt ttttgcagaa tttgaaagag
    51361 cagctagaga agctggtgta gcagatgcga caagcgcaca aggagaagcc acaactaata
    51421 ataccattcc tcgataaaag ctttcatccc aactccaacc taggaaaaag tgggggagaa
    51481 ggatcattag aggaacagca agcaaaatca attttacata attattttcg attcgttcga
    51541 taaatgaagc tgtttgagtt ggcgtattct gcgcttcttc tactaattgg ataatcttag
    51601 caaagacagt ttcggaactc gtttttgtta ctcgaatcgt gatgggattt ccttgattca
    51661 gcgttcctgc atacatctca tcgcctaaat atttttctac aggcacagat tcaccgctta
    51721 atgcagactc atcgatcgtt gcctgtgtgc tttctaaaaa gccatctatc ggaacagctg
    51781 ctcctttagc gacaaatacc aagttatcca cctttagttg agaaacatct acttcttgca
    51841 tttgtccatt ttcttttaat aattgggctt tttgtggttg aagattcatc aaagcagtaa
    51901 tttctttttg acttttatta gttgtgtatt cctctaatgc accgcttaaa caaaagatga
    51961 atgtcaacat tgctccttca aagtaattac cgatcaaaca ggcaccaata gcagctaaag
    52021 ccatcaataa atccacattc agtgtccgat cattccacaa ttctttcaag ccttccgatg
    52081 tttgtttata tccaccgatg ataatggcac tgatataaaa aagtggggtg atggtaagag
    52141 agaatcggtc gaagataaaa ccgaagatca taaataaaag gcagccgatt gttgctgcaa
    52201 aagctttttt ctgattagtc atcgttatta gacctccttg tcaaaacaag gatagcataa
    52261 taaaaatgta attgataata ataacagcta aatgagaacg ataatggtta tcatttggtg
    52321 attaagcttg atgttgtttt tattatttat aatgattatt attaaagaaa ataaaaaacc
    52381 agaacatttc tcaaataaga gaagtgttct ggttttttct acattgcaat aaaccaataa
    52441 ccaatcaaaa tgataccaag gataatacga taccagccaa aagcggtaaa gtcgtttttc
    52501 ttcaaatagc ttagtaagaa tttgatagct aagacggaaa ccacaaaaga tacgaatgtt
    52561 cctacaagaa gaacaacagt ttcttccatt ttgaatgaat tcccttcagc taaatatttg
    52621 acgatcttca agccgcttgc gccaaacatg acaggaatcc ctaagaaaaa tgaaaactct
    52681 gtagcgacaa aacgcgaagc accaatcaag atagatccta ggatcgttgc gccagaacgg
    52741 gatgttccgg gaacaagtgc caagacttgg aataacccaa caagaagagc tgcttggtac
    52801 gtaaagtcat tcagagaagt ccaacgaggt gttttgtttt tgttgcgttt ttcgacaata
    52861 ataaaggcaa taccgtaaac gatcaaaaca atggcaactg gtaagaactt gtggaaattt
    52921 gcttctaacc aatcatccaa aggcaaacca atgatgatgg ctggtatgca agcaacgatc
    52981 actttaaacc aaagtgtcca cgtatctttt ttttctaccg ttgatttact tggagaaaat
    53041 gggtttaact tgtgaaaata aagaacgacg acagccaaga tcgcccctaa ttggataacg
    53101 acattgaaca tcgacataaa ttgtgaactg gcatccagtt tgatgaattc atctgctagg
    53161 atcaaatgac cagtactact gatgggcacc cattccgtta ttccttcaat aataccgaga
    53221 atcactgctt tgataatatt tgcaaaaaac ataaattcct tccttcccaa ttaagataca
    53281 tcttatagta taccgttatt ttttgagaaa cccaatctgt tttttaatat taagaaaaac
    53341 ttgagaaata aagcagataa gtacacgaag agaaaaaaga agacaaagcc ctcgttcaga
    53401 ctttgccttt tatgattctc tggtaaaaat taaaatatcc ccattataga aacgagtcgc
    53461 gactatgtgt tttcccgtta tcgatataag acgtatcatt gatgccgtgg atagaaaatc
    53521 gaccgtttgc ataagtgacc tttgttacgc tgccatttaa aagcccagca cgaacaggct
    53581 gtttaggatc gatcagatta agcaaaaagg cgatcgtcaa accatgagat acgactaaaa
    53641 catttccacc accatttttt tcgcagtgat gggcgatatc ttcaaatcct gtccatacac
    53701 ggtctcgcaa tgtttcataa gtttccgccc acccagcggt atctaagttg tatacagcat
    53761 tagcaatttg ttcaaaagaa acttctgtgg taaacatttc atcatatgtt ttaaaattca
    53821 atacacgagg gatcacaccc cacatttcca tgtcatatcc accttcaaga gacccgaaac
    53881 accattctct aatccttttt tcaataaaat aaggaatttc ttttccctta gaatgttctc
    53941 ctagaataat acgggcagtt tcgattgcac gaccactgtc actagaataa gcaaatgaaa
    54001 agtcgacatt ttttaacccg cgacctaaat aatgaatgcc ttcttgtcct tgctttgtga
    54061 ggggtgtatc acaccagcct tgtactcgtt caattgtatt gaacatcgtt tttccgtgtc
    54121 gtacgatata aagtgtagtt tctgtcattt tattctcctc ctaagtcgtt ttaacgattg
    54181 aaaaaaggat ttgtcctttt ttcctgttca atcgtcgttg catcgccatg tcctggatag
    54241 actgcaaatt catcaggtaa tacaaacagc tgggtccgaa tactatgaag taattgttct
    54301 aaattccctg tatacagatc ggttctaccg atacttcctc taaacagggc gtcgccagaa
    54361 acgacaaaat catcaaaaat aaagctgacg cttcctatag aatgccctgg ggtagggacg
    54421 acgcgaaaag acatgtttcc taaagtataa gttttcattt caaattcata ttctgcaggc
    54481 gagacaataa catcagcaat atcatcatgt ctaccgagtc cagaaagatt caaaatcgga
    54541 tcacctagcc atttttgctc tagtgggctg acataaactg ggatttgata atacgcgcgt
    54601 aaaggttcca ctgcaccgat atgatcgtag tgagtatggg taagcaaaat cgctaccggc
    54661 tgttggtttg tttttttgat ttcttgtttg attcgttccg catcttctcc agggtcaatg
    54721 atcaaaagtg cttcttcgtt gtagaccaaa taacagtttt cttcaatcat tcctgttttt
    54781 aatctttcga tatgcatgtg cattcctcct tgatagccaa aacttctttc atccattagt
    54841 ataacgcatt cattgctagg aaacaaaaac aggtacttat ttctctttac ttatctgaat
    54901 tatcagacta tatgaaaagt atttggatat tctaaagaga aggaaagtaa gttttcatag
    54961 cattttaata tgtccttgtt acattagatt catccaaaaa tccaaattct atatcctata
    55021 tccctgccta atcagcaggg tgttttttta tgtttggaaa gagagctctt tttgctaaac
    55081 taggtttttt caggtaatct tagagagaaa gaggtggtgg aaatgatcaa acagtcaaaa
    55141 atatctgtgc ggcacttagt agaatttatt ttgcgacgtg gaagtatcga caatcgtaaa
    55201 aaaagtaacc atactgcatt agaaggagcg agaatccacc gcaagctcca aaaagatgcg
    55261 ggagaaaact atgaaaaaga agtcttttta aaaacaacag ttcaattaga tgaatattac
    55321 ctgatcgttg aaggccgtgc agacggtatt ttcaaaagag acggtattta ttatatagat
    55381 gaaatcaaaa cctccgaacc acgatttgaa gatttagaac cagaacaagt ggaacttttc
    55441 tttcatcaag caagggtgta tgcctatatc tatagtcaag aacatgattt acaagaaata
    55501 aatctgcaat tgacttactt ccagacggca gaagaagtga tcacgagaaa ggttgaacat
    55561 cagacaagag agcagttaga aacttttttt aaaaagctga ctgaagatta taaagaatgg
    55621 cttgtttttc aagaaaactg gcgcactgta cgcaatgcct ctttgatggc tttgaaattc
    55681 ccccatgatg agtataggaa aggtcagcgt gaactagcgg tagctgctta taaaacgatc
    55741 cgtacgaaac agaaattgtt tgtagaagca cctaccggaa caggaaaaac aatctctacg
    55801 ttattccccg ctttgaaagc tgtaggggaa gaagagggag aaaagatctt ttatctaacg
    55861 gctaaaacaa taactagaca agtagctgaa gatgctatga cagcattaaa agatacagga
    55921 gctgaagtaa aaagtgtcac tttgacggcg aaagacaaga tctgcttttt agatgagacg
    55981 acctgtaatc cagatcagtg cccatatgcc aatggttatt acaatcggat taatgaaggt
    56041 ttatgggatc ttttgaatca tgagaatcag atcactcgtg aagtcatcga aacttatgca
    56101 agaaagcata cactttgtcc tttcgaacta tcattagatg tcagtatctg gtgtgacgta
    56161 atcattgggg attacaatta tttatttgat ccaactgttt atttaagacg tttttttgaa
    56221 gatgaaaaaa acgaggacta tctgtttttg attgacgaag cacataattt ggtgaatcgt
    56281 tcccgagaga tgtattcggc agaactatct tatgaaaaaa cgaaacgaag taaggaagct
    56341 ataccaaagg aattcaaaaa acttcatcgc cgtttcaaca aactattaaa agaatttgat
    56401 tcgatccgag aaatagcaaa agaagatcat tgggattatc atcatcaaaa agcaccggct
    56461 gaatctttag taaaggcagg ttatcaatta agcgaaaaaa ttaaagaatg gctggcagaa
    56521 tttcctgagc acccatcaca agaacaattg cttccttatt attttgattt gcttcatttt
    56581 ttgaaggtca gtgaatttta tgatgatcac tacgaaacaa cagtcgaaaa aacgtatcgc
    56641 gatctgatag taaaagagtt ttgtattgat ccaagcttgt ttcttgagca gtcgttggat
    56701 aaaggaaaaa gcagtattct tttttctgct agtttttcac cactctctta ttatcaggaa
    56761 acactggggg gacaaaaaag tctggcttac aaactgccga gtcctttccc agaaggaaac
    56821 cagcaagttt tcatcgcaaa ttacctagag acgacttatc gaaaaaggga acacagtctg
    56881 gcaaaattag tagagacgat tcatgctttt gctgatggaa aggtcgggaa ttatttcgtt
    56941 ttctttcctt cttatcaata tttagatctt gcggtggaag cattcagaca gcaatatccc
    57001 gacagcaatg tgctaatcca agaaacggac atgaatgagg aagaacgcga acgattttta
    57061 acaaaatttc aaacaaatcc cgctgaaaca ttgattggat tttgcgtact tggcgggatt
    57121 ttttcagaag gaatcgattt gagaggggac cgattgatcg gcagtatgat cgtaggtgta
    57181 ggtttgccgc agatgaatca tgagcaggaa ctgatcaaat catattatga tgagaaggaa
    57241 caccttggat ttgcttacgc ttatcagctt cctggaatga acaaagtctt gcaagctggt
    57301 ggtcgagtga ttcgtggcat ggcggatcaa ggaatcgttg tattggcaga tcaacgattt
    57361 tctagttttt cttatcgacg tttattccca caacattggc aaacagcaag agaagtacga
    57421 agtgttgggg aattggaaga aggaatacag cgtttttggt tatcaaaaga agcagtaaat
    57481 caaaaaaacg aggaatgaca gacgtcagcc tcgttttttg attaacgata gttagtgaat
    57541 tgcaactcga ttggtaagtc aagtttacgt aataaagcaa tcacagcttg tagatcatca
    57601 cggttcttac ctgtcactct aatctgatct tcttggattt gtgcttttac tttcagtttt
    57661 tcatttttga tagctgtagt gattttcttc gcgttttcac gatcgattcc gttgaccaaa
    57721 tcggcagttt gtcgagcttt gccacctaga gcgtgttctg attcggaaaa atggatattt
    57781 ttgattggca catttctttt gatcagctta ccaaataata catctttgat ctgttcgatt
    57841 ttgaaatcat catcgctcaa tacaactaga ttgctgttct ctaatttgat ttctactgta
    57901 gaatccttga agtcaaagcg attggacaat tcttttgttg tttgctgaat cgcatttttt
    57961 acttcttctg tattgacttc tgatacaatg tcgaagctag catcttttgc cataggaacc
    58021 ctcctgatat acatataaat atttaccata catctatctt atcataggat tagggcagat
    58081 tcttacctgt ttgctaaaat tcttctcatt tgagttcgaa gtattttatt ttaatagata
    58141 aactgttatt cttacatttg gaataccaaa gtaggggaga ggtgcttgtg agtaaaatta
    58201 tgactgcgta tcataaaaat gaaagattaa agaaaatagt ggttattttg ataaccggat
    58261 tgaccgctgc ggtcgcttta aattattttt tgattccagc caatgtcttt tctgcaggga
    58321 tgaatgggat cgctcaaatc atttcctcgc ttttatatga atggtttcat atcaagatag
    58381 acacaggaac atttattttt cttttgaata tccctgtgtt tgtattaggg tttctcaaag
    58441 taggaaaacg agcaacgatc cttagttttg tgaatgttgt atgtgtatcg gtgatgacga
    58501 tgctgcttcc agtcggacag atcactgata atattttgat gaatgcccta gtaggcggtg
    58561 ttttattagg tcttggcgta ggattatctt taaaaatggg ctttacaact ggtgggatgg
    58621 atatcatctc tcttgtactt tcacaaacaa ctggaaaaac agtcggaaac tacatgctga
    58681 ttctaaatgg tatcattgta gtcatcgcag gcttcttctt taactgggaa agtgccttgt
    58741 atacgattat ttccatttat gcaatgactc aagtaattga tgccatccac acgagtcatc
    58801 aaaaagtaac agcaatgatc gttaccgtca agccagaaga ggtaacagaa gaactagcaa
    58861 aacggatggt acgtggaatg acattactgc cgtctgtcgg tggctattca aaaaaacaag
    58921 ggaacatgat catgatggtc atcacccgct acgaaatgta tgatcttgat cagatcgttc
    58981 atgaaatcga cgacgatgca tttatcaata ttttaccaac tcattcagta tttggccggt
    59041 ttgcaaatga aaacgaacaa aaaatgtatc gaacaactgg cgtattgcca gaaataaaaa
    59101 atcgttcaca aaaaaaatga ggaatcaggt ttattttaga ataaatttaa tctattacat
    59161 ctaataagaa catttttata aatttaaacg agaatcttac ttctaaattc tcatttacaa
    59221 gcagctaaaa aaagagtatg ataaataaaa atcgtgttaa ggagtgtcgt acgatgagcg
    59281 aagcaaacca agaacggtct ggacaagaaa tagaactaat caaagaacgt attcttgctg
    59341 ccttggaaat ggtgattgat cctgaattag gaatcgacat cgtcaattta ggcttgattt
    59401 atgatataga attcaatcct gaaaatggag aaacagtcat taagatgact ctaacaacta
    59461 tgggctgtcc attagcagat attttaacag aatctatcca tgacgcatta aaagaagtgc
    59521 cagaagtaac gaaagcagaa gtcaaactag tctggtatcc agcatggacg acagataaga
    59581 tgagccgtta tgcacggatt gcgttgggga tacgatagaa atcttggctg actagctatg
    59641 agaagcaaat aggaaaaaca aaaaagcctt ttatcctttc tagaagaaaa gagataaaag
    59701 gtttctttgt ttataaaatt ttgtctgcta tattataagt taattaa
//
LOCUS       DAZKKW010000008        59417 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.008, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000008 DAZKKW010000000
VERSION     DAZKKW010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 59417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59417
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.008"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1626
                     /locus_tag="ACG8PO_000457"
     CDS             <1..1626
                     /locus_tag="ACG8PO_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0205814.1"
                     /translation="KNQIIDIIFMLFLSFAYVIPLFMSKGIYHSVNQDTYFHLSRIIG
                     LDNVWSSPVNFNNFDHHGTMMNIFYPWLTLYPAFLFYKMMGNLVLSYNIYYFFITFLT
                     MVVSYFSMKQIKNNRYISLLFSIIYTFSAYRAIDIFHRASLGEAVALTFLPLILMGCY
                     EIYIRDYQKWYWLSIGMTLVVYTHLLSVAMVSVFIGGTLFLSFYFWDQKIARLLSLLK
                     ATVLTFFLSAGFLIPFIQQSRAQELKVPLGKELSGMAPSDMLTHILNNNYNNYTIGLF
                     LFLGLIGAFIFIKKLTADDLFIFFLGVFVLFCSTNLFPWQLFNHTPVKSLQFVWRLNG
                     FSSLFIAYTMSIVIYYIFSTKNNSWKIMVFTFLALILHLSGVSNSIHANKDSLTLIAP
                     EDAVAIAENYNHTDYANKESIYHPDMINNDQYLLGNQEINPTTHFMSNKLTIELDNSN
                     NKNTVLTTPIYRYKGQVASINGKLVQTKLSKFGTTELTIPPGINKVVITYQYTKLAIA
                     SRYLSIVTLILFLLYRFTFSKQKQPRREVQHSH"
     gene            complement(1771..2511)
                     /gene="truA"
                     /locus_tag="ACG8PO_000458"
     CDS             complement(1771..2511)
                     /gene="truA"
                     /locus_tag="ACG8PO_000458"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFQ0205815.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(2702..3337)
                     /locus_tag="ACG8PO_000459"
     CDS             complement(2702..3337)
                     /locus_tag="ACG8PO_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HFQ0205816.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            complement(3484..4704)
                     /gene="sstT"
                     /locus_tag="ACG8PO_000460"
     CDS             complement(3484..4704)
                     /gene="sstT"
                     /locus_tag="ACG8PO_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HFQ0205817.1"
                     /translation="MSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSCLKAIAPI
                     LVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPITLQETVSE
                     KAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSESTKELVDQI
                     SIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLFVALVVYP
                     FLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKESYSISIP
                     LGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGVAGGSLLL
                     IPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELGARKRSGE
                     KIKLKDCLDGLSER"
     gene            4923..5921
                     /locus_tag="ACG8PO_000461"
     CDS             4923..5921
                     /locus_tag="ACG8PO_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0205818.1"
                     /translation="MFLSNFFVRCLFLKKIKKESYGFLTIFVACIMMVLQRILISAVT
                     IPTYATNPGRFYIYTFLQTSVVIGANLIPLYMGYQYQKIARLKVLHYLSRFAFIFLIA
                     TLVCNIFFYVQAGSLNIRDYWLILTPISQNYFTYAVSCTLLFCSIPYTVGFLNKLSKT
                     TIKYLLLFLTIGLVGCSTLFNKDPFALQNGQSILWIFYLFLMGYGLKEWNWKEKVRYK
                     LPQVFISLIVLFGLIILMTQISLIIRGNTDTALRFSKPYSLFAMYYTFSSFLFLEMLS
                     DKLGIKGRGNFLAVFLIVTQVICNFPLVSYRVSTFMKREFPNSGKAWLINIGQFFI"
     gene            6117..7952
                     /locus_tag="ACG8PO_000462"
     CDS             6117..7952
                     /locus_tag="ACG8PO_000462"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFQ0205819.1"
                     /translation="MQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFFFLL
                     LLISNRFWYALTATLIINLLLTISTVIKMEMREEPVFPSDLKMLTGLSELLSMVSPVL
                     LIVGGLILLLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHKNSP
                     SFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMKKYN
                     TKADEINQTRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTGGLM
                     LSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIAIHP
                     FTAALYRRKQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQSNT
                     ETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAVKKF
                     IQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAATLNK
                     EVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVFVTE
                     DSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSATWATQKIKD"
     gene            complement(8012..8728)
                     /locus_tag="ACG8PO_000463"
     CDS             complement(8012..8728)
                     /locus_tag="ACG8PO_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HFQ0205820.1"
                     /translation="MKKRILVTIGLAFLLAGCTNGNESKKESTKETAKATVATTTTTS
                     TESKSTAASTETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNAD
                     KSAQLEAFMTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEW
                     SESGTGQADYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFN
                     ETANNELKNGFNQIVYGQAN"
     gene            complement(8955..9497)
                     /locus_tag="ACG8PO_000464"
     CDS             complement(8955..9497)
                     /locus_tag="ACG8PO_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFQ0205821.1"
                     /translation="MNIVEEKFVQETKQADIDHHKPTAGAMVGHVLANFKLEEMKLIQ
                     AQLYVLGTSSQELSTLFSEYADQEARWFNKISHELVIENEIVPSTLEEVMRYGKIYEH
                     GQNKYLPASTMLENFAKDFDFQNVFITRAIKLAEKEEKYGLQQLLVELLSFNKEIIYQ
                     VQRLQGKTVRQDLDEEDDDD"
     gene            complement(9502..9729)
                     /locus_tag="ACG8PO_000465"
     CDS             complement(9502..9729)
                     /locus_tag="ACG8PO_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HFQ0205822.1"
                     /translation="MKKIIMQLDTLACPSCMQKIEHAVSQQPGVSNTKVLFNASKIKA
                     NFDPDEVHPEQLAAVVEKLGYEVQSAKVKEA"
     gene            complement(9808..11688)
                     /locus_tag="ACG8PO_000466"
     CDS             complement(9808..11688)
                     /locus_tag="ACG8PO_000466"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFQ0205823.1"
                     /translation="MKLQKWIQEHRNHITALSAVLIIFGFIGKFTGFVLFYNIALAIA
                     SIIAVIPIMIHAYQAVKVKVISIDLLVTIAVLGAFMIGEYKESAIVTFLFLFGSYLEQ
                     KTLTKTRESIKALTEMAPSTASVVQEDGTTEEVEIDDVEEGDIVLVKTGGAIPVDGRV
                     IDGKGYADEAAVTGESRQVVKISGDEVFSGTILADGYVTIEATKVGDDTTFAKIIELV
                     EEAQDTKSHAEKFIDRFSQYYTPTVLLIALVVGLISRDFRLAITVLVLGCPGALVIGA
                     PVSNVAGIGNGAKRGILVKGGEAMDTFSKIDTFVFDKTGTLTKGTTAVSTIKNYTEDE
                     GDSLALVARLETLSDHPLGRAVIHYAQEKDIDFQQLAVKDNQTIKGQGITAEIDGHTV
                     CAGNTKLIAAHKLSLTPKQVQDLHQLQQTGSSVIIVAIDKKITQIIGVSDVIRPEVAE
                     QLAKLKQAGAKQLVMLTGDNQMTADYVADMLGIDEVHAELLPDEKVQFVKKYQEQGLR
                     VAFIGDGINDSPSLAAADIGIAMGSGTDVAIETSDVVLMQSNFESLVHAYRLAKKTVL
                     NTRENVVIAIGVVLFLLNGLFAGFIYMASGMFVHEASILIVIFNAMRLLYFGHESKEE
                     KS"
     gene            complement(11877..12491)
                     /locus_tag="ACG8PO_000467"
     CDS             complement(11877..12491)
                     /locus_tag="ACG8PO_000467"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0205824.1"
                     /translation="MKLTFKPLEKKDYKKAIYFAVEGMNFERYMENELLLNLYGRYFL
                     YMELNRATQILALYAEDELAGVLLAEIKGEKKKHRSFGQQIYVKIFDFLQNIFAKESK
                     GAYETANEQMLAAYLKKHSPDGEIIFLAANPDLKTKGIGTKLLTEFEKREKGKEIFLF
                     TDSGCTYQFYERRGFERLKEKISKIKILDKEIDLRCMLYRKIIQ"
     gene            12633..13193
                     /locus_tag="ACG8PO_000468"
     CDS             12633..13193
                     /locus_tag="ACG8PO_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0205825.1"
                     /translation="MKKTDRRVKKTEKALAETLSTLLVNKKIQAITIRELTETADVHR
                     STFYTHYKDIYDLYDQLESNFFRDLNEILSYDPAHSYEELYTRLIDYVYANTFLYRLF
                     ILNHGDISFQKKVKQVIEENYLNIWLYEDKKTIITEEMRFFTTYHVQGCLSVLNRWVE
                     SNYKYSKKELLELLQKLNDHIEKIMP"
     gene            complement(13498..13812)
                     /locus_tag="ACG8PO_000469"
                     /pseudo
     CDS             complement(13498..13812)
                     /locus_tag="ACG8PO_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
     gene            complement(14066..15701)
                     /locus_tag="ACG8PO_000470"
                     /pseudo
     CDS             complement(14066..15701)
                     /locus_tag="ACG8PO_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294751.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(16024..16503)
                     /gene="rlmH"
                     /locus_tag="ACG8PO_000471"
     CDS             complement(16024..16503)
                     /gene="rlmH"
                     /locus_tag="ACG8PO_000471"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFQ0205826.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            16931..18220
                     /locus_tag="ACG8PO_000472"
     CDS             16931..18220
                     /locus_tag="ACG8PO_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HFQ0205827.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            complement(18259..18864)
                     /gene="ytpR"
                     /locus_tag="ACG8PO_000473"
     CDS             complement(18259..18864)
                     /gene="ytpR"
                     /locus_tag="ACG8PO_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtpR family tRNA-binding protein"
                     /protein_id="HFQ0205828.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(19069..19533)
                     /locus_tag="ACG8PO_000474"
     CDS             complement(19069..19533)
                     /locus_tag="ACG8PO_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFQ0205829.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            complement(19555..19875)
                     /locus_tag="ACG8PO_000475"
     CDS             complement(19555..19875)
                     /locus_tag="ACG8PO_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFQ0205830.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            complement(20019..21098)
                     /gene="pepA"
                     /locus_tag="ACG8PO_000476"
     CDS             complement(20019..21098)
                     /gene="pepA"
                     /locus_tag="ACG8PO_000476"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HFQ0205831.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            21298..21648
                     /locus_tag="ACG8PO_000477"
     CDS             21298..21648
                     /locus_tag="ACG8PO_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFQ0205832.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            21871..22818
                     /locus_tag="ACG8PO_000478"
     CDS             21871..22818
                     /locus_tag="ACG8PO_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFQ0205833.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(22875..24293)
                     /gene="pepV"
                     /locus_tag="ACG8PO_000479"
     CDS             complement(22875..24293)
                     /gene="pepV"
                     /locus_tag="ACG8PO_000479"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HFQ0205834.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            24442..25287
                     /locus_tag="ACG8PO_000480"
     CDS             24442..25287
                     /locus_tag="ACG8PO_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HFQ0205835.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            25291..25731
                     /locus_tag="ACG8PO_000481"
     CDS             25291..25731
                     /locus_tag="ACG8PO_000481"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HFQ0205836.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            25781..25975
                     /locus_tag="ACG8PO_000482"
     CDS             25781..25975
                     /locus_tag="ACG8PO_000482"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0205837.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            26111..26796
                     /locus_tag="ACG8PO_000483"
                     /pseudo
     CDS             26111..26796
                     /locus_tag="ACG8PO_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
     gene            complement(27204..27893)
                     /locus_tag="ACG8PO_000484"
     CDS             complement(27204..27893)
                     /locus_tag="ACG8PO_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0205838.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(27868..29997)
                     /locus_tag="ACG8PO_000485"
     CDS             complement(27868..29997)
                     /locus_tag="ACG8PO_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HFQ0205839.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLLENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            complement(30045..31382)
                     /locus_tag="ACG8PO_000486"
     CDS             complement(30045..31382)
                     /locus_tag="ACG8PO_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HFQ0205840.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            complement(31634..31993)
                     /gene="rplT"
                     /locus_tag="ACG8PO_000487"
     CDS             complement(31634..31993)
                     /gene="rplT"
                     /locus_tag="ACG8PO_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFQ0205841.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            complement(32046..32246)
                     /gene="rpmI"
                     /locus_tag="ACG8PO_000488"
     CDS             complement(32046..32246)
                     /gene="rpmI"
                     /locus_tag="ACG8PO_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFQ0205842.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            complement(32318..32842)
                     /gene="infC"
                     /locus_tag="ACG8PO_000489"
     CDS             complement(32318..32842)
                     /gene="infC"
                     /locus_tag="ACG8PO_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFQ0205843.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     misc_feature    complement(32886..33011)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            33162..33794
                     /locus_tag="ACG8PO_000490"
     CDS             33162..33794
                     /locus_tag="ACG8PO_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFQ0205844.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            complement(33796..34701)
                     /locus_tag="ACG8PO_000491"
     CDS             complement(33796..34701)
                     /locus_tag="ACG8PO_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFQ0205845.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(34786..35445)
                     /locus_tag="ACG8PO_000492"
     CDS             complement(34786..35445)
                     /locus_tag="ACG8PO_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFQ0205846.1"
                     /translation="MSVIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFY
                     ANPEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEV
                     RVYDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYD
                     YYKELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVA
                     I"
     gene            35761..36705
                     /gene="mvk"
                     /locus_tag="ACG8PO_000493"
     CDS             35761..36705
                     /gene="mvk"
                     /locus_tag="ACG8PO_000493"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004496 - mevalonate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HFQ0205847.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            36698..37675
                     /gene="mvaD"
                     /locus_tag="ACG8PO_000494"
     CDS             36698..37675
                     /gene="mvaD"
                     /locus_tag="ACG8PO_000494"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004163 - diphosphomevalonate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HFQ0205848.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            37688..38773
                     /locus_tag="ACG8PO_000495"
     CDS             37688..38773
                     /locus_tag="ACG8PO_000495"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004631 - phosphomevalonate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HFQ0205849.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            38770..39834
                     /gene="fni"
                     /locus_tag="ACG8PO_000496"
     CDS             38770..39834
                     /gene="fni"
                     /locus_tag="ACG8PO_000496"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HFQ0205850.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(40083..40886)
                     /locus_tag="ACG8PO_000497"
     CDS             complement(40083..40886)
                     /locus_tag="ACG8PO_000497"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HFQ0205851.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(40906..41748)
                     /gene="kduI"
                     /locus_tag="ACG8PO_000498"
     CDS             complement(40906..41748)
                     /gene="kduI"
                     /locus_tag="ACG8PO_000498"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HFQ0205852.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            complement(41764..42771)
                     /locus_tag="ACG8PO_000499"
     CDS             complement(41764..42771)
                     /locus_tag="ACG8PO_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HFQ0205853.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            complement(42778..43422)
                     /locus_tag="ACG8PO_000500"
     CDS             complement(42778..43422)
                     /locus_tag="ACG8PO_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFQ0205854.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            complement(43522..44280)
                     /locus_tag="ACG8PO_000501"
     CDS             complement(43522..44280)
                     /locus_tag="ACG8PO_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HFQ0205855.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIKTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            complement(44407..45558)
                     /gene="fucO"
                     /locus_tag="ACG8PO_000502"
     CDS             complement(44407..45558)
                     /gene="fucO"
                     /locus_tag="ACG8PO_000502"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008912 - lactaldehyde reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HFQ0205856.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEASVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            complement(45640..45951)
                     /gene="rhaM"
                     /locus_tag="ACG8PO_000503"
     CDS             complement(45640..45951)
                     /gene="rhaM"
                     /locus_tag="ACG8PO_000503"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0062192 - L-rhamnose mutarotase activity
                     [Evidence IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HFQ0205857.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            complement(45953..46798)
                     /gene="rhaD"
                     /locus_tag="ACG8PO_000504"
     CDS             complement(45953..46798)
                     /gene="rhaD"
                     /locus_tag="ACG8PO_000504"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008994 - rhamnulose-1-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HFQ0205858.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            complement(46812..48068)
                     /gene="rhaA"
                     /locus_tag="ACG8PO_000505"
     CDS             complement(46812..48068)
                     /gene="rhaA"
                     /locus_tag="ACG8PO_000505"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HFQ0205859.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            complement(48065..49525)
                     /gene="rhaB"
                     /locus_tag="ACG8PO_000506"
     CDS             complement(48065..49525)
                     /gene="rhaB"
                     /locus_tag="ACG8PO_000506"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008993 - rhamnulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HFQ0205860.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            49678..50547
                     /locus_tag="ACG8PO_000507"
     CDS             49678..50547
                     /locus_tag="ACG8PO_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFQ0205861.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            complement(50673..51572)
                     /locus_tag="ACG8PO_000508"
     CDS             complement(50673..51572)
                     /locus_tag="ACG8PO_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0205862.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(51737..52561)
                     /locus_tag="ACG8PO_000509"
     CDS             complement(51737..52561)
                     /locus_tag="ACG8PO_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFQ0205863.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPEIP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            complement(52572..53867)
                     /locus_tag="ACG8PO_000510"
     CDS             complement(52572..53867)
                     /locus_tag="ACG8PO_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HFQ0205864.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            complement(53883..54362)
                     /locus_tag="ACG8PO_000511"
     CDS             complement(53883..54362)
                     /locus_tag="ACG8PO_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HFQ0205865.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            complement(54368..55396)
                     /locus_tag="ACG8PO_000512"
     CDS             complement(54368..55396)
                     /locus_tag="ACG8PO_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HFQ0205866.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLKGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            complement(55413..56519)
                     /locus_tag="ACG8PO_000513"
     CDS             complement(55413..56519)
                     /locus_tag="ACG8PO_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFQ0205867.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            complement(56748..59345)
                     /gene="adhE"
                     /locus_tag="ACG8PO_000514"
                     /gene_synonym="adhC"
     CDS             complement(56748..59345)
                     /gene="adhE"
                     /locus_tag="ACG8PO_000514"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HFQ0205868.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
BASE COUNT        17508 a        12386 c        10446 g        19077 t
ORIGIN      
        1 aaaaaccaaa taatagatat catttttatg ttatttctaa gttttgctta cgtcatccct
       61 ctattcatgt ctaagggaat ctatcatagt gtcaatcagg atacttactt tcatttatct
      121 cgaatcatag gactagataa tgtatggagt tctccagtaa acttcaataa cttcgatcat
      181 catggaacaa tgatgaatat tttttatcca tggcttactc tttatccagc ttttttattt
      241 tataaaatga tgggaaatct tgttttaagt tataatatct actatttttt tatcactttt
      301 ttaacaatgg tcgtttctta tttttctatg aaacaaataa aaaacaatcg ttatatttcg
      361 ctgttatttt cgattattta tacattttcc gcctatcgtg ccattgacat attccatcgt
      421 gcttcattgg gagaagctgt cgctttaaca tttttgcctt tgatactaat ggggtgttac
      481 gaaatttata ttagagatta ccaaaaatgg tactggttgt ctattggcat gacgctagta
      541 gtgtatactc atttactttc tgtcgctatg gtttctgtat ttattggagg aacgttgttt
      601 ctttcttttt atttttggga ccaaaaaatc gctagactct taagtctttt aaaagctacc
      661 gtcctcacct tttttttaag tgcgggattc ttaattcctt ttattcaaca atctagagca
      721 caagaattga aagtcccact gggaaaagaa ttaagtggta tggctcctag tgatatgctg
      781 acacatattt taaataataa ctataacaac tatactattg gattattctt atttctcgga
      841 ttgattggtg cttttatttt tataaaaaaa ttaactgctg atgatttatt tattttcttt
      901 cttggtgtat ttgttctttt ttgttcaacc aatttatttc cttggcaact ttttaaccat
      961 acaccagtta agagtctaca atttgtctgg cggttaaatg gtttttcttc tttatttata
     1021 gcctatacga tgagtatagt gatctattat attttttcta ctaaaaataa ttcatggaaa
     1081 ataatggtct ttacgtttct tgcactaata ttgcatctct caggagtaag caattctatc
     1141 catgctaaca aagattcatt aacacttata gcacctgaag acgctgttgc tatagctgaa
     1201 aattacaatc atactgatta tgcaaacaaa gagtcaatct atcatcctga tatgattaat
     1261 aatgatcaat atttactagg taaccaggaa ataaatccaa caactcattt tatgagtaac
     1321 aaacttacaa ttgaattaga taattcgaat aataaaaata ctgtcttgac gacacctatt
     1381 tatcgttata aagggcaagt ggcttctatt aatggcaaac tagtacaaac aaaattatct
     1441 aagtttggca caactgaatt aactattcct ccaggtataa ataaagtcgt tattacttat
     1501 cagtacacaa aactagcaat tgcttcaaga tatttatcta tcgttacttt gattttattt
     1561 ttactttatc gatttacttt ttctaaacaa aaacaaccga gaagagaagt acaacattct
     1621 cactagattt ttatttaaga aagacttttt agctgtacac ataaacaggc taaaaagtct
     1681 ttttttatat ttctttaatt tgcttcatac attatttaac atcgatattt atactgtgaa
     1741 acatgcgttt catccattat caaagtattt ttatcgataa taaacttcat ccaaaaacag
     1801 accttgtgca gggacggttt ctcctgcatg ttgccgcact ttgctttcaa atatttcatc
     1861 gattgtttct atcggcatgg cacctgtgcc gatttctagt aatgtcccca tgataatccg
     1921 caccatttta tataagaatc cgtctccgac aaaagtaaaa tgaagcattt ctccctttcg
     1981 ttcgatagat aattcatcaa tcgtacgcac tgtggatttt tttgattttt tcaatgcaga
     2041 aaaaccaatg aaatcatgtg tacccactaa tttttcgcaa gctgcattca gcttgtccat
     2101 atccaactct tgagggaaat aaaagctgtg gtttcgctca aatgctgaag gaatcacgtt
     2161 gttccaaaca taatagctgt attttttgcc caccgcatta tatcttgaat ggaagcgctc
     2221 aggcatttct tcgattcttt tgatgacaat atcccgcgga aggtaacgat tcataaagtc
     2281 gatcatttct gccagtccaa tttctgtgtt tgttttaaaa tttgctactt gtcctcgcgc
     2341 atgggtgcca gcatctgttc ttcctgatcc aataatttct atctcttcat ttgtcatctg
     2401 atgcaatact ttttcgattt ttccttgaat ggttttatcg tcatctccaa gtctttgcca
     2461 tcctgaatat ctttttccgt catattcgat tgtcaacttg atgtttctca tattttagtg
     2521 gctcctctgt cttccatttg gtcttctctc taccttttta agattgtagc acaaaatata
     2581 gctccttgct tctctaagac agaagctata caagaaaact gtggaaatat atatttcaga
     2641 aaaacaaaaa acagccaccg gatgaagtat ccgctgactg ttcaatttga cggctcaagt
     2701 tttactgttt gagatgcaag atactcaacc aggcaatggg cacataatta aagaaaaaac
     2761 tgattctttg gcaaaaacct attttattca gtaatggcca agaatttata gaaggaattc
     2821 ttgccagtcc ataaatcgcc gcaaaaagca aactgatcac agacagtcca aggtataagt
     2881 ggcttacgtc catttgccca ttttttcgat agatcaagaa aagaagaact ggaaacaata
     2941 aaaaaccact gtaacctaaa cctgatccga tattatggac ccatgtcgag acattccacg
     3001 aagattcatg ggtattgata ctgaacaaac ctgtaaagat acaatcacct ataccataga
     3061 acgcgatggc tgctgtaact agggtagcta acacacgaga tgtactaaca aatgcagcat
     3121 acaatgcagg caatgacagg atgaaaaaga tccctgaaac cacagaccaa ataagaaacg
     3181 cttttcttac aggactggaa acctctccaa atacactgat cacgctcttc atttgatcta
     3241 attcaggata atacaatcct aatacgtacg gagtcaaaaa atcactcagc acgcctaata
     3301 ataaaaagaa aaaaccaaat ttacgtaaaa aatccatgat acgcctccaa taacaattac
     3361 ctatgctttt aattgttatt ataaacgact ttatcttgat ttaacagaaa cctctattta
     3421 cacgaacatc tatataactc attaaaaact cgaatcattt taaaaatact aaatatctaa
     3481 cgttcaacgt tcacttaatc catctaaaca atctttcaat ttgatttttt ccccacttct
     3541 tttacgcgcg cctagctctc ctattgcagt aaacaacaga tcacttgacg aatttaaggc
     3601 agtttccatt gaatcctgga taacaccgat aataaatcca ataccgacga tttgcatcgc
     3661 gacatcatta gagataccaa acaaactaca tgctaatgga atcaataaca gtgaaccgcc
     3721 cgcaacacct gaagctccac aagcagctaa agcagacaaa atacataaaa ggactttcaa
     3781 aacaaaaggt gcttcaatcc ctaaagaatg tacagcagtc aacgtcatca aggaaatggt
     3841 aatcgcggca ccgcccatat tgatcgtagc acctaacggg atcgatatgg aataagactc
     3901 ttttgtcaat ttcaatcttt cagccaacat catgtttatc gggatatttg cggcagaact
     3961 tcgggtaaag aaagcgggaa gtgcgctttc tttcaaacaa aagaaaatca atggataggg
     4021 attttcttta ataaacaaaa acgtgaggaa tgggtacaca actaatgcga caaacagcat
     4081 cgtaccgatc aaaacaagta aaagctgcag atactctgcc aatccagcga ttccagtcgt
     4141 agcaattgat tgataaacca atcctaaaat ccctatcgga gcaaatgcaa taatcacttg
     4201 tacgacttgt gacaacgcta tagatatctg atccaccaac tcttttgtcg attctgaacg
     4261 tgctcgaaaa gctagtccca acaatacagc ccagaataag acgccaaggt aattcgcatc
     4321 aataagagat tgaacaggat tttgtgtgac attcgtcaac atagtagata aaacactttc
     4381 caaactttgc ggtgcttttt cagatactgt ttcttgtaaa gtaataggaa cagtaaaaat
     4441 tgaacttaca ataactgcta caagtgcaga aagcaatgtt cccactaaat ataagatcaa
     4501 aataggcttc acgaatgtct tattcccaat tttatgcttg gcaattgacg ccatcgtcaa
     4561 gaaaaaaacc agaataggtg cgattgcctt caagcaactg acaaacaacg ttcccaatat
     4621 tgaaataaat gtccatgtgg ggaaaaagat accaaacaat attccgacta aaataccaac
     4681 aacaatacgt tgaactaacg acatatccaa aaacttctct aacaaatgac caccctttca
     4741 caattttaac aatcattgta tcattttatg caaagaaact aaaggtataa ttttctaaaa
     4801 attctaacaa tacaaacaac catttcttgc taattttgta ctttttctgt ctacacgttg
     4861 ttttttgaga tattctaagt taggatgtta ttaatgggca acttatcagg aaacgttttc
     4921 ttatgttttt gtccaatttt tttgtgaggt gtttattctt gaaaaaaata aagaaggaga
     4981 gttatggctt cctgacaatc tttgtcgctt gtataatgat ggttttacag cgtatattga
     5041 tttcagcagt cactattcct acatatgcaa caaaccctgg tcgattctac atctatacat
     5101 ttttacaaac ttccgttgtt attggtgcaa atttgatccc actttacatg ggttaccaat
     5161 atcaaaaaat cgcccgttta aaagttttac attatttaag tcgttttgcc tttatattcc
     5221 taatagccac actagtttgc aatatctttt tttacgtaca agctggcagc ttgaatattc
     5281 gtgattattg gcttatccta acacctatca gtcagaatta ctttacttat gctgtttctt
     5341 gtactttgct tttttgtagt attccttata cagttggatt tttaaataaa ttatcgaaaa
     5401 caacaattaa atatctacta ttatttttaa caattggatt agttggctgc tctactctat
     5461 ttaataaaga cccgtttgca ttacagaatg gacagagtat tttatggatt ttttatttat
     5521 ttttaatggg ttatggtttg aaagaatgga actggaaaga aaaggtccgc tataagcttc
     5581 ctcaagtatt tatcagcttg attgttttat tcggtttgat tattttgatg acacaaattt
     5641 cactcatcat cagaggaaat acagatacag cgctgaggtt ttctaaacca tattctctct
     5701 tcgcaatgta ttatactttc tcttcgtttt tatttttaga aatgctatct gataaattag
     5761 gtataaaagg aagaggaaat tttcttgctg tatttttgat tgtcactcaa gttatatgta
     5821 atttcccttt agtttcttat cgcgtatcta catttatgaa acgtgaattt ccaaactcag
     5881 ggaaggcatg gctgataaat atcgggcagt tttttatttg atatttatta gcagtgtccc
     5941 tcctagttat ctttttattt tatatacaaa aaatccgttt cattcatcgt ttcatcgaat
     6001 acttcgcttt tgattcgatt gaacaattaa ctcagcgttg gaaaaaaaga attcattggc
     6061 tttttgttca taaaagttat tttttagttg cagcattttt ttactgcttt accttcgtgc
     6121 agattttcgt tttagaacca aaagaaggtt ggaaagaaac tatccaggtt gccttaaaaa
     6181 tatttactca gcgacaagca cccatgatcc tgacaatcat cattattatg ggatttttct
     6241 tcttactatt gttgatttcc aatcgttttt ggtacgcctt gactgctacc ttgatcatta
     6301 acttactgct cactatttcg acagtaatca aaatggaaat gcgggaagag cctgtctttc
     6361 cttcagattt aaaaatgctg accggattgt cagaactatt atcgatggtt agcccagtct
     6421 tattaatcgt gggcggattg atccttctcc tattattgat tactagtatt attgtacaac
     6481 gtagactaca aaaacagtat tccttgaaga ttcattggaa acgtcgattt atcagtatcg
     6541 ctgtattatt aggatgtttt tcgggtgtct ttttcattaa tcacaaaaat tcgccttctt
     6601 tcttgctgtt taatttattt aaagtaaaca agactttctt taaccagcaa ggcgccgtaa
     6661 aagataacgg accagtcatt caattcctaa acaatatcga cattaaaatc atggaagaac
     6721 ctgctggcta ttcgaaagaa aaaattaatg gaataatgaa aaagtacaac acaaaggcag
     6781 atgaaatcaa ccaaacaaga agtgactggt taaacaatca gacgatcgtt ctcaatttaa
     6841 gtgaaagctt cagtgatcct gcacgtgtcc ctaatctgac agtacctaca gaccctattc
     6901 cttacatcaa aagcattata acagaaggaa caggaggatt gatgttatcc gtcggttatg
     6961 gtggcggaac agctaacatg gaatgggaag gactgacagg cttgagtatc agtaacctgt
     7021 ctgcttcgtt agttacacct tatacccagc tagtggagcg tcagaaaata agtccaaata
     7081 ttaccgatct attcgatgaa aaaatagcca tccatccttt tacagctgct ctttatcgca
     7141 gaaaacaagt gtttgataaa tttggttttg acaggttcta ttatttagac agtccggata
     7201 aattaactta tacggataaa atagaacaaa gcccacgcat atcggatgat tcagcataca
     7261 aagaaacctt gaaagcttta caaagcaata cggaaacgtc acaattcatc caattatcca
     7321 ctatgcaaaa ccatatgcct tatgatgatt actatagtga attgaactac actgctgaag
     7381 gaactgctgt cattcctaat agaagaaaag aattacaaac ttatatgcaa ggccttcact
     7441 ataccgatac agcagtaaaa aaattcatcc aagaaatcga caaaatcgat aaaccgatta
     7501 cctttgtttt ttatggtgac catttacccg caatctatag tgggaatgat tcgaaaaaat
     7561 atggattaga acagcaccag acagattatt tcatttataa caacaagtat agtcgtgaac
     7621 aagcagctac gttaaataaa gaagttgttg ctcctaataa ctttggtgct atggctcttg
     7681 agcatgcgaa tatcaaagta actccttact atgcgttgtt gacagaagta tctaatcatc
     7741 tacctgcatt aacaattgat cctacagaaa gcttgtctaa tcgttttaat ggaaaacagg
     7801 tctttgttac agaggatagt caaattatcc aagaaaaaaa tttaactgag aaacaaaaag
     7861 aaattctaga agattatcgt ctgatacaat atgatcttgt tgcaggtaac cagtacagtg
     7921 caacatgggc gacacaaaaa ataaaagact aacaaataaa ctgagcaagg ggttgtgata
     7981 aaagacatgt cacaacccct tgtttatctc attaatttgc ttgtccataa acgatttgat
     8041 taaagccatt tttcaattca ttattcgctg tctcgttgaa gtaaagataa ttatcgggat
     8101 tcccctgatt ttgttgtgtc accaatactt ttggctgatt ttgctgaaac ccgaatagat
     8161 agacgtgctt tttcagatat ggttctgtct ctgcatcgga atagacggct gtgatttgat
     8221 agtctgcttg acctgtccca ctttctgacc attctgcaga aacaggcact ccaccaatag
     8281 ccatcttcca ctccccgttc aaaatagctt gagggacttt aagaccatat aagtccacac
     8341 tcatttgatt cgtataactt ttgtattgtt gtcccattgt ttggccccat ttggtcataa
     8401 atgcctctaa ttgtgcggat ttgtctgcat tccacagtgt ttgttcaact tgttcgctgc
     8461 tttctgaaga ggaagatgaa gcctctacag aagaacttga agtagatgtt tgagttgttt
     8521 cagtcgtttt ggaagactct gacgaagtag aggtatctgc tgtttctgta cttgctgcag
     8581 ttgacttgct ctctgtgcta gttgttgtag tagttgcaac tgttgctttc gctgtttctt
     8641 ttgttgattc ttttttcgat tcattcccgt tggtacaacc agctaacaaa aaagctagac
     8701 caattgttac taaaatcctt tttttcaatt aaaaaactcc tttcgattac tcccacttct
     8761 ttattctatc aaataagcaa tgtgatttta ttgatttttc gtattttctc atcaatcttt
     8821 caaatcttct aaaaactgag aagcacccgc tgatgttgtc tgtaatacat cagcgggtgc
     8881 ttcttttatt ttaatcttcg tgattttcca atttacctca cgctttatat ttgtatttga
     8941 agaaacatag acttttagtc atcatcgtct tcctcatcca aatcttgtcg gacagttttt
     9001 ccttggagtc tttgtacttg gtagatgatt tctttattga aacttaaaag ttcaacaagc
     9061 aattgttgca agccatattt ttcttctttt tcagctaatt tgattgcccg tgtgataaag
     9121 acattttgga aatcaaaatc ttttgcaaag ttttccaaca ttgttgaggc tggtaaatat
     9181 ttgttttgac catgttcgta aatttttcca taacgcatga cttcttctaa agtagacgga
     9241 acgatttcat tttcaatcac taactcatga gagattttat tgaaccatct cgcttcttga
     9301 tcagcatatt ctgaaaaaag ggtacttaat tcttgcgaag atgtacccaa aacatacaat
     9361 tgtgcttgga tcaatttcat ttcttctaat ttaaaatttg ctaatacatg ccccaccatg
     9421 gcgccagcag ttggtttatg atggtcgata tctgcttgtt ttgtttcttg aacaaatttt
     9481 tcttccacta tattcattga tttacgcctc ttttactttt gctgattgga cttcataacc
     9541 taatttttcc acaactgcag ctaattgttc agggtggact tcatctggat caaagtttgc
     9601 tttgattttg ctcgcattaa acaaaacttt cgtattgctt accccaggct gttgagaaac
     9661 cgcatgttcg attttttgca tacagcttgg acaagctagt gtatctaatt gcataataat
     9721 ttttttcatc gtagttcttc cttccttttt cttgatagtc ttattctaga ttacagcttc
     9781 cataaaaaaa ttgattctta tcaagtttta cgatttttct tctttgcttt cgtgtccaaa
     9841 atacaataaa cgcatcgcgt taaagataac gatcaagata ctagcttcgt gaacgaacat
     9901 cccactagcc atgtaaataa acccggcaaa taagccgttg agtaaaaata agacgacacc
     9961 aatggcaatc actacatttt cacgggtatt taaaacagtt ttcttagcca agcggtaggc
    10021 atgaaccaac gattcaaaat tagattgcat caagacaacg tcactcgttt cgatagcaac
    10081 atccgttcct gatcccatag caatcccgat atcagccgca gccaaagagg gagaatcatt
    10141 gattccatct ccaataaaag caacgcgcaa tccttgttct tggtattttt tcacaaattg
    10201 aaccttttcg tctggtaata attcagcatg gacttcatca atgcctaaca tatcagctac
    10261 ataatcagca gtcatctgat tatcacctgt cagcatcact aactgttttg ctcctgcttg
    10321 tttcaactta gctaattgtt cagcgacttc tggtcgaatc acatctgaca cacctatgat
    10381 ttgagtgatt tttttgtcga ttgctacaat aatgaccgag ctgcctgttt gttgtaattg
    10441 atgtaaatct tgtacttgct ttggtgtgag agaaagtttg tgagcggcaa tcaatttggt
    10501 gttgcccgca caaactgtat gaccatcaat ctcagctgtg attccttgtc ccttgattgt
    10561 ctgattatct ttgactgcta attgttgaaa atcaatgtct ttctcctgag cataatggat
    10621 cacggcgcgc cccagaggat gatcggataa cgtttctaat cgagccacta aagctaagct
    10681 gtccccttca tcctctgtat agttcttgat agtggataca gcagtagttc ctttggtcaa
    10741 tgtaccagtt ttatcaaaga caaaagtatc gattttggaa aaagtatcca ttgcttcacc
    10801 gcctttgact aagatgcccc gtttcgctcc attaccgatt cctgctacat tcgaaacagg
    10861 cgcaccaatg actaaagcgc caggacaacc aagaaccaaa acagtgattg ctaaacgaaa
    10921 atcacgacta atcagaccaa caaccaacgc aataagtaaa acggttggag tataatactg
    10981 agaaaatcga tcaatgaatt tttccgcatg ggatttggta tcttgtgctt cttctactaa
    11041 ttcgataatt ttggcaaagg tcgtatcatc tccgacctta gttgcttcaa tcgtcacgta
    11101 gccatctgct aaaatcgttc cagagaagac ttcatctcca ctgattttga cgacttgtcg
    11161 tgattcacca gtcactgcgg cttcatccgc ataccctttc ccgtcaatga ctcgtccgtc
    11221 cacaggaata gctccaccag tttttactaa gacgatatcg ccttcttcca cgtcatcgat
    11281 ttccacttct tcagtcgttc catcttcttg gacaacacta gcagtagatg gggccatctc
    11341 ggtcaaagcc ttgattgact cccgcgtttt ggtcaatgtt ttttgttcca gatagctacc
    11401 aaacaaaaac aagaatgtaa cgatagctga ctccttgtat tctccgatca taaaggcacc
    11461 aagcacagca atagtaacga gtaaatcaat tgagataact ttgactttca ctgcctgata
    11521 cgcatggatc ataataggta tgaccgcaat aatagaagct atggctagag caatgttata
    11581 gaacaagaca aaaccagtaa attttccgat aaaaccaaaa ataatcaaca cagctgataa
    11641 ggcggtgata tgatttcgat gctcttggat ccacttttgt aatttcatct ttctccctct
    11701 tttcctttca ttcttacgag actaagtata gatacttatc catcataaaa aattgaccac
    11761 tatcaagttt agtcaaaaag gcggttttct tttggtagaa tacactacaa aaaatagtta
    11821 gcaagaagac tcactaaaag tgatcttgcc aactattgtc gtttctatcg aaattttcat
    11881 tgaatgattt tcctgtacag catacatcgg agatctattt ctttatctaa gatttttatc
    11941 ttgctaattt tttcctttag tctttcaaat ccacgtcgtt catagaattg atacgtacag
    12001 ccactatctg taaaaaggaa tatttctttt cctttctctc ttttttcaaa ttctgttagc
    12061 aacttggtac cgatgccttt tgttttcaaa tcaggatttg cagctaaaaa aatgatctct
    12121 ccatctggcg aatgcttctt taaatatgct gcaagcattt gttcatttgc tgtctcgtaa
    12181 gctcctttac tttctttagc aaatatattt tgtaaaaaat cgaatatctt aacatatatt
    12241 tgctgtccaa aagaacgatg tttctttttt tctcctttta tctccgccag caaaacacct
    12301 gccaactcgt cctctgcata caaagcaaga atttgagttg ctcgattcaa ctccatatac
    12361 aaaaaatatc ttccgtaaag attcagcaga agttcatttt ccatatatct ctcaaagttc
    12421 attccttcaa cagcaaaata gatagctttt ttgtaatctt tcttttctaa gggtttaaaa
    12481 gttagtttca tcatttcgcc tcctcaactt caatgattgt gttatactta cattataata
    12541 gacacagtgt ctactatcta taatcaatca tctataagtt tgtcgattaa gcgacagctt
    12601 tgctttgata tgtcggataa ggagataggt aaatgaaaaa gacagataga cgcgtcaaaa
    12661 aaacagaaaa agctttggct gaaacactat ccacattatt agtaaataaa aagattcaag
    12721 caatcactat acgcgaacta acagaaacgg cagatgtaca tcgttcgact ttttatacac
    12781 attataagga tatctatgat ctatatgatc aattagaaag caactttttt cgggatctaa
    12841 atgaaattct ctcttatgat ccagcccata gctatgagga actatacact cgcttgattg
    12901 actatgttta cgcaaatact tttttatatc gcttatttat cctcaaccat ggtgatattt
    12961 cgtttcagaa gaaagttaaa caagttattg aagaaaacta tctgaacata tggctatatg
    13021 aagacaagaa aacgattatc acagaagaaa tgagattctt tactacgtac cacgtccaag
    13081 gttgtctatc cgttctcaat cggtgggtag aaagcaatta caagtactcc aaaaaagaat
    13141 tattagaact cttacagaaa ctgaatgatc acatagagaa aatcatgcct tgaataaaaa
    13201 catactaagc ttagttaaga agaaccgtaa ccttctcgta taacaactga atcttattta
    13261 gccaaattgt ttactgaaga ttttttgagt aaaatttcta taattcctac ttattgaaaa
    13321 gtagtttgta gtgctgcttc tccctctcta agtgatatat cttgttgtac caatcaaatt
    13381 cacctcatca ttaataaatt acttttaggc taatttatta caacttatag gttgaagctg
    13441 taaccaattt atagtcatat tatattttta tcatccagtt tattgaattc ttctcgttta
    13501 cctcaattca ttttcaaggg gaatcagctc atcatctaac attttacgcc atgctcgctt
    13561 catttcttgt gggtatttaa atggtttcgc ttctggatgt ttggctttat atccttcgta
    13621 atccttactg ttgataattg ctttcatatt aggaattgga tcttcacctg gcatatattg
    13681 cagattagaa gcatgtaatt ttttctcaga aacaaaccac tcattggaaa agacatgaat
    13741 caaatcatca cttacttgtg taaaatagcg acgtttaatg acaaagatat cttcattgct
    13801 atctagtgta ccttcatttc tttgtgctta aattcaaaga taattttctc aataaagatt
    13861 tttgattcca gtaagattcc tttactcctt ttacaacata gcgttgtcct ttgttttgaa
    13921 acatcttctt cccctctttg ccattaaaaa tagcccaatt ctctataaag aagattaagc
    13981 ttcattatta taatataaag tttaaatttg gtttattact tactttttct tattctctag
    14041 taagctaata ttgaaaaaaa tgtaatcaga tcattatttt tatatgttgt aacttcctga
    14101 taagaaaact aattaaaaga aagaggtaac tacataaact aattaaaaaa gctaacttca
    14161 tagttggagg ataaatggaa gaaacttcaa tgattgtttc tccttgtgtt ccttcaatcg
    14221 acactagtcc tattatcaat ctctcttttt tgtccattta cttttgcgac tgttccccaa
    14281 taatcaaata caggaagcgt tattttttgt cctttagatt catgtactgt atagactatt
    14341 ttcgaatcca tcacttgata aggcatagta tgccattcct tgccaattct tacttcatga
    14401 ttctgcaaat gttctttgta ttgtaatgct tccgtgggtg catagtcata aagtcctccg
    14461 ggaaggagtg tcgtgtaacg aataagattt tcttgattca ttaccaagtg atctctctga
    14521 gtgacaactt tatgttcata gtttgctttt gttgcagcag atacagccaa cagtaataaa
    14581 acaattgccg ttatattaac tacttttctt tgcttagctg atgtgctctt catgaaaacg
    14641 aacgataaaa tcaacgagcc aaacagtact tgaaagccta agattcgcca aggaaattga
    14701 atcatttgaa tgggtgtatt ttgtaataaa gaccatggaa aaaatttggt ggataagagc
    14761 caagagacta ccgcactaag gtaaatcact ttttctttgc gattaaatcc tttaaaaaag
    14821 aagagtggac agatcaaaga aataatacca atgattccta ccgtaaaggt tgtcaggtca
    14881 ttattcatcg caccaatcaa actatccgag acatttaatg cttggtgttg taaggtcgtt
    14941 ttatctggct cattaatcga ttgaaccatg atttgtcgga acatcggata ccaaaagtat
    15001 gctgttaaaa gaatcgtcat tcctactgcc tttgacaact ccacaatcaa ccggatgctt
    15061 tctttttttc ttgtttcact tactacaaag ccgcctaaga ctagtaagcc aacggctatt
    15121 acactcataa aagtggaaag aacatgtgta taaatgagta agctcatact gactgctaat
    15181 ggaattgcac atggcttttt ctcataaatc agtgcgtaag cataatacat aactatcggt
    15241 ataaccgtaa gtgcaatcgc ttcagcaata gcagaacgat gataaatatc aatcgtacgg
    15301 tacccataaa atgtatataa acagctaaat ataaaagccg catcgattct ttttaaaaat
    15361 tttagtccat aatgaaacat cagcatgata gtacaaacat tcaatagcca aacatataag
    15421 atataactaa cgattaaatt attacttatc caataaaaaa tgactgccgg aaaaaaggtc
    15481 aagtagggat aaaaataatt cactccgctc ccataatgat taaacgtcgt aaaattgatt
    15541 ggaccagaaa aaattgaaga taaccctttg actctagcaa aatggaaggc aatatccccc
    15601 gatgtagaaa tagtatgaac atttctaaaa taattgattg aataaagtcc agcaataatt
    15661 gctaggagaa tggacaatat tatttttttc tttacttgca ttacttaatt atatactcct
    15721 ttcatggatt gctttatcta gtataactta aattttaaat aaatcgttat ttatttttac
    15781 tctcacttaa aaaagcacta aaattataag agagggaggt aatggtctga acaccaagaa
    15841 ttgttccaac atcgaattgc ttaactaatg agttcaaagc ttaattatgt ggctcttata
    15901 tttaccgagc gtggattgcg atctctttag cgaaacaaac atctttcata aaaaaaacag
    15961 ccatatccgc tttcttggat atgactattt actactactt tactatcttt cattcttctt
    16021 gtctcactta tgataaggcg cccccgcatt gatccggaag gcacggtaga tttgctcgac
    16081 aaggatcaga cgcatcaatt gatgtggata cgtcattttt ccaaaagaga tctgagcatt
    16141 gcttcttttc atgacggctt cgctgagtcc taaagagccg ccgataacaa aaacaaggtg
    16201 gctttttcct tggataccta attggtcgat ttgtttagca aaagattcac ttgtaggatt
    16261 tttcccttca attgctaagg catagacgta ttcttgttct ttgattttcg ctaagatccg
    16321 ttccccttct ttttctttta cttgtctcat ttgcgcttcg cttaaattct ccggggcttt
    16381 ttcgtcagga acttcgatca attcgatttt tgcataggca ttcagccgtt tgacatactc
    16441 attgatacct tggatcaggt atttttcttt taattttcct actgagataa ttttgatatt
    16501 catgttttct ctcctttggt cttcatgctc actttgttta ttataatcaa ttttttcgtc
    16561 tatatgttta ttttggctat ttttgttaaa aagatttccc caacagttat gcacaacatc
    16621 cacaggttta tccacagaaa acagtgttat tttaccttat ttcatcacat tcattaggca
    16681 aacatacgtt ttataagaat ttatccactt tatacacagg ctgtggatat gttttatccc
    16741 ttatttttcc aatcctatcc gcgtagtttt acacagtatc tgtggaaaaa gaaaatcaaa
    16801 tattttcatc tgaaaacaat gatttttcct aaagtttttc ataaaaagtt cacagtctca
    16861 aagtagacta aagtcaacaa ataagactat actaacagta gataaatgaa tgtaattgga
    16921 ggaggaacac atggatagaa aaaatgtgac accaaagatg aaaaaaagta aaaataatag
    16981 cctctggcgt aagttaggtc tggggcttgt tggagggatc gttggaggac tactgacagc
    17041 tggcattttt tatgcagcga tgggcactgg caacaatagt tctactgcta caagcggcaa
    17101 tcagaattct gccggtgaaa cagttgtgga aaatgtcaaa gtcaatgtag attctgatat
    17161 caccaaagcg gtagataaag ttcaagacgc tgtcgtttct gttatcaact tacaaagtca
    17221 aaaccaatca agcggttttg gcggactatt cgggcagcaa gaagaaagta gcagctcaga
    17281 tgatagtaat ttggaagctt atagtgaagg aagcggcgtg atctacaaaa aagacgggaa
    17341 tactgcttat gtagtcacga ataatcacgt agtagatggc cagcaaggtc ttgaagtctt
    17401 gatgaaagac ggtacgaagg taaaagctga attagttgga acagatgctt attcagattt
    17461 agcggtgttg aagatcaatt cagacaaagt cgaaacagtt gcctcttttg gtgactcaag
    17521 tgctctgaag gttggtgagc ctgcgattgc gattggttcc ccactaggat ctgaatatgc
    17581 aaattctgtc acttcaggaa tcatttcttc cttgaaccga caagtgacaa gtacgaatga
    17641 atcgaacgaa acagtcaata tcaatgcgat ccaaacagat gcagcaatca accctggaaa
    17701 ttctggtggt cctttagtta atatcgaagg acaagttatc ggtatcaact caagtaagat
    17761 cgcaagtact tctgaatcta cttctaatgt aagcgtagaa ggaatgggat ttgctattcc
    17821 aagtaacgat gtagtgaata tcatcaatca actggaaaaa gacggaaaag tgacacgtcc
    17881 agctctaggt atcacaatgg tcgacttatc tgccgtttct actcagcaac aagaacaaat
    17941 cttgaagatt ccttcatctg taactaacgg tgtcatcgtg acaaatgtcc aaacagctac
    18001 accggctgaa aaagctggat tgaaacaata cgacgtcatt actaagatcg atgacaaaga
    18061 agtttcttct ggtgtagaac ttcaatctgc tttgtataag aaaaaagtgg gagattctgt
    18121 caaaatcact tactatgaag gctcgaaaga aaaaacaact actgttgaat tgacgatcga
    18181 tcaatcggca ttaaaacaaa atcaaaatag tgaaaattaa taaaaaagag gttgtgacaa
    18241 agacttgtca caacctcttt attttggaaa tgcttctccg actacggcat cctcaggcaa
    18301 tactaagatt cctttttttg ctggagcatt tggcaaatgc aattcacgag cggaacagat
    18361 cattccatag ctatctaccc ctcgcaaagt tcctggccag atcatcattc catctggcat
    18421 catagctcct ggttttgcaa cgacaacttt ttgtccagct tcgatattgg gtgcaccgca
    18481 aacgatttgc aatactgtac cattgtctac ttctgtctca gtaatcgata aatggtcaga
    18541 atcaggatgt gggacgcatg tttttacgta cccgacaacg atcttcggtt catgatcagc
    18601 ttcaagctgt tcttcgaatc ctgcttcttt gatcaatcga ttcaatttcg ctaattcctc
    18661 atctgttaag cttacttggc cgttcccttc gattgttaga tgatcggaga tatggaaaaa
    18721 gttccaagca acaacttgac cttgttcatc tttgatccga gcaacattcc atttgcgttc
    18781 aacagtgttt tctgttcctt tatcgtcggc tataataacc atcaaggtgt ctccgacgtg
    18841 tgctttgtta taagcaaaaa tcatgttctt tcctccatta tcctttgctg agatttatct
    18901 taacaaaaaa ttgcattcac tgcatgaaat ttttttcatg ctttatttcc gaagaagcct
    18961 cttctggaac accatttatt cgtaacaagg gactgtgaca agacttttgt cacagtccct
    19021 ttactgtttt agttaattga ttaaaaaggt cgtacaagtt ccacttgctt aattacttgg
    19081 ctgttcttct tcgtcagaaa cgatcaagac atcacaaggt gaatgacgta tcacgtagct
    19141 ggctacactt cctgtcataa agcgttcgac tgcatttaga cctgactgcc caaccatgat
    19201 tagatcgatc tcatattttt ttggcaattt gactgccatg gattcacgaa cagaaccata
    19261 tgcaataatt ccttctatat ttgtgtaatc aacagatttc ccatattctt tacattcttc
    19321 gatcatttcc ttggctgttt cagtttgctg atcgactaga ttttgatcaa taggtgcata
    19381 gcccataaag ttataaaatt gctgatcgat tacgtttgct acatatactt tcccattatt
    19441 acggcgagcc acttctaccg cttttttgaa agcagcaagt gcttgttcac taccgtcgat
    19501 acctactaaa atattcttat attcttgatt catcttgacc cctcctaaca gatgtcagcc
    19561 ttttgttaca ctttctagga acgattcgat ttcctgtttt gttttacgat ctttattcac
    19621 tagacgacct aattcttttc cgtcttggat cacgacaaaa cttgggatac caaaaatgtt
    19681 ccattcagcg gctacatcta tgtacttgtc acggtctact tcaataaatt gccaatcaga
    19741 aaagtcattt tcaatttcag gcatttgcgg tttgataaaa cggcagtctc cgcaccagcc
    19801 ggcagtaaag aaaaatacac tctttccagt ttctacgtag cctgctaatt cttctaaact
    19861 atttgggata atcattcttt gtcctctact ttctgtttat gtatttttca attaaattat
    19921 acacctttct ttcgaactaa caaacattga tcctcttttt ataaaaaaaa tctggcaaaa
    19981 agcaatcttt tgccagattt tatagtttca ctatcgcatt attttccgta tacgatcgta
    20041 tttactgttg atttatccaa acctttcagt gtttgaatca acatttctcg tgcagcttca
    20101 aaatcgcgaa tactgaacat cgtttgatgc gtatggatat aacgtcccac tacgccaatc
    20161 actgtacttg ggattccttc gttttgtgta tgagcagcac ctgcatctgt accgcctttt
    20221 gaaacaaagt attgataagg gatattgttt gtttcagcga tatccagcaa gtactcgcgc
    20281 aaacgaggaa gcatgatcaa acctggatct tggatacgca tcaatgtacc ttctcctaaa
    20341 tgtccgaatg tgccattttt cgtcactgta tcatctgctg cagagcagtc tactgcaaag
    20401 aacagatctg gcttgaattt attgacagaa gctttagaac cacgcaagcc tacttcttcc
    20461 tggacattcg cgcctgcaat caatgtatga cctaatgttt cattttgcaa cgcttctaaa
    20521 gcttctaata ccattgtaca gccatagcgg ttgtcccatg atttactgat aatattcttt
    20581 ccgttggctg ttttgatcgt ttcagcgtaa ggaacgattg tgtctcctgg aaggacaccg
    20641 taactcattg cctcttctct tgattcaaac ccagcatcga acaaaatatc ggttacttct
    20701 actgattttt gtccgcttgt tcctcgcaat aaatgtgggg gaacggaaga agaaatacat
    20761 ggataattct taccagtaga ggttttaagt gtaaaacgct gagcagaaac aacgtaagga
    20821 ttccagccgc ctagaggtac gactttaaaa agcccgtttt cttggatttg tgtcagcatg
    20881 aatccgactt catccatatg tgctgcgacc attacacggg gtgcatccgc ttcttcatga
    20941 tggcgcagac caaaaatgcc gcccaaacca tctaactgta actcatctac cagcggttcc
    21001 atatgacttt ccatataagc gcgaacatct tgttcaaaac cgcttgttcc ttgtaattct
    21061 gttaactctt tgattcgttg aaatgttttt tcttccatat gctattcgtc taaatttcag
    21121 actaaactcc tctcagactt atattgatct gcggataaaa tatgattttt tctgcagacc
    21181 gatcgtattt attatagctt attttttctg gtagcggtta tttatttcaa agaaaatatg
    21241 agagtgtggt aaaataagca caaggagtaa caagtgagaa gaaggaggta tgtccacatg
    21301 tatgaagaaa gcgaactaag ctattttaaa ggcgggttag cagttggaat cggtctagga
    21361 ttattgagcg gggtagcttc aacgatttgg tatcatcgca aaaaagtatt gtcagcagac
    21421 caagtacttg aaaatatcaa gtcatctttt ctaaaagaag gtgcaatcga aggttcttgg
    21481 atctcttttg aaaaggaacc tattaggaaa ttcgccgttc ggtcgagtgc ttatcgggga
    21541 ggtctttctc gaatagaaga tggcgaaact gtttattatg aatttttagc agatgctcat
    21601 acaggaaccg tattagaaat ttcaagaaaa aaaggtgcaa cagattaata cgtcaaaaaa
    21661 gtgagagaac gaatacgcac ccactattca aaagagaggt atcatgtcct tcttttgtag
    21721 tgggtggttt tttagataga gctttccaaa catatattcc agtattttct ttttaaataa
    21781 atgatattta gcgggaaaaa ctagcatttc ctttgattat gaggtatttt taatttagct
    21841 tttgttttct atagttaagg agagtgaaaa atggaacaac aagaaaaaat tgctgttctg
    21901 attccttgtt acaatgagga agcaacaatt tcaacagtca ttgcagactt taaacgagaa
    21961 ttaccagaag cagatattta tgtctatgat aataattcga ccgatcgaac atacgaattg
    22021 gccgttcaag gtggagcaat cgtaaaaaaa gaacctcgtc aaggaaaagg aaatgtgatc
    22081 cgccaaatgt tctttgatat cgatgccgac tattatttaa tggtagatgg cgatgatact
    22141 tatcctgcag aagctgtaca tggtttgctc gagaaattac gttctggtga agcagatatg
    22201 gtcattggtg atcgcctatc aaatggaacc tactttgatg agaataagag acctttccac
    22261 gattttggaa ataaccttgt tagaaataca attactagaa tgtataaaac aaaaattctt
    22321 gatgtcatga ccggctatcg aggattcaat cgtatctttg tcaaaagttt tcctattatg
    22381 agttctggtt ttcaaatcga aacggaattg acgatccatg ctttagataa aaaattcaag
    22441 ttcgttgaaa tcccaatcga ttatcgtgat cgtcctgaag gcagcgaatc aaaattgaat
    22501 acattctctg acggattcaa agtgatcatg atgattatta aaatgtttaa agattataag
    22561 cctttgcttt tctttgggat ttggaccatt ttcttctttt tatttggatt ggtcacgggg
    22621 attcctgtga ttcgcgaata tatgctgaca agctttatta cacgcatccc ttctgcgatt
    22681 ttaagtactg gattgatgac tttagcactt ctttcattag taacagggtt gattttggat
    22741 acagtcgtca cgaacgctaa gaaagaatat gaattgaatc tatatcatgt ttatcatgaa
    22801 taccacagta gaaagtaaaa aaaggatcaa gactgcaaaa ataatttgca attcttgatc
    22861 ctctttttaa ttaattattt cgccaaacga tagatggcat ctgcatagat tgctgttgct
    22921 ctgaacaagt catcaagact cataaattcg tttgcttgat gcattgtatc agtatatcct
    22981 gggaacatcg ctccataagc aacgccacgt ttcaacaaac gtccgtaagt tccgccaccg
    23041 atgatttgtt cgtagccttt ttctccagtg tggtcttcat agacttgcaa taatgtagcc
    23101 accaatggat catccattgg tacgtaatgt ggttccatta cgcgagcacc tcgagtaact
    23161 gttgcgcctt cttgccccac tgtttgttcg atgtcacttt gcaatccgtc tactgttaca
    23221 ccttttggaa aacggaaatt catattgatg aagttcgctt cctctgtacc attttcttca
    23281 aaagcaaata gtccagcatt cattgtcaat ttgcccattt tttcatcttc ataagcgact
    23341 cctagttttt ctccataaaa atcttcatgg acataagcag ctgctacatg gataaattgt
    23401 ttagcagatc ctaggaattc atagttatct aagaacaatg ccaagaaaga acctgcattg
    23461 ataccagatt gtgggcttgc accatgtgcg cctttaccaa ctaattcgat ttttacatac
    23521 gtattgtccg cttcaatcgt tccgcttact ggatttgctt cgatgaattg gtaaaatgct
    23581 tcttccatcg cgatagctgc atctgcactt ggtacttgaa gtgctgcagt tgctgttcct
    23641 ggcaccatat tttcgcgaag tccggaaaca aaagatttca agacataatc tcctgcattg
    23701 tcgcctttga agcgtaaagc taaagaaaca tttccttttt caccattgat gattgggaat
    23761 tcagcatctg gtgagaaacc aaaatctggt tcttcttcat gttcgaaata ataagccata
    23821 tcgccccagc cgctttcttc atcgctgccg actacgaagc gaactttttt tgatagtttc
    23881 aagtcaagtt ctttaatgat cttcatcgca tagtaagctg ccatacttgg acctttgtca
    23941 tcgcttgatc cgcgtgcaaa gatctttcca tccttgatta ctggttcata aggatctgtg
    24001 tcccagccat ctcctgcagg aacgacatcc atgtgtccaa atatccctag cgtttcgtct
    24061 ccttcgccaa gatcaatatg ccctgcatag ttatcgacat tttttacttt aaatccatca
    24121 cgttcgccat aagctaacat atgtttcaat gcgtcgcgag gtccaggtcc aaatggtgca
    24181 tctggtgtta cttttgagtc atcgcgctcg ctgttgacac gtaaaagatt tttcaagtct
    24241 tcaagcaagt cttctttacg tgcttctact tctttttgcc aatcaattgt cataaagtta
    24301 atcctccact tatttaagtc ttcgtgttca tgataccaca aattacagaa aaaaatagtt
    24361 caaaaaatct gacaattcct tttcttatct gtttttctct aaaaaaaata tacgctataa
    24421 tgagtaaaag gaggttttta gatgctgata aacgaacaac tagtgaaaag agtcatcaca
    24481 cctcgtccaa aaatttcaca taaaggaaca tttggacgta tcgttttgat tggcggcaat
    24541 gaacaatatg gcggagcaat cattatgagt gctttagctg ccgtacacag cggagcgggt
    24601 ctcacgactg tagtaactga tgaaaaaaat cggagtgctc ttcacagtca tctgccagaa
    24661 gcaatgattg tagtttggac ggatactgac cagtttcttt ccgtaataaa gcaagcagat
    24721 gtactcttga ttggtcccgg attgggtact tcttctaaga gtttggaacg tttcaaacaa
    24781 gtactagaaa accaatcaaa agaacaatgg ctggtgattg atggctctgc tttaagttta
    24841 cttgctgcta atcatctttt gctaccctat cccgaacaaa ctgtacttac acctcatcaa
    24901 atggagtggc aacggttgag ttctctaccg atacaagaac aacgggataa agaaaacaaa
    24961 aagaagcaac aggaaatcgg tgctgtgctc gttttgaaaa gtcatgagac aaaaatttat
    25021 ggtcaccagc acttctacac caatccttta ggttctgctg ctatggcaac gggcggcatg
    25081 ggtgacactt tagctgggat gatcacagga tttttagccc agtttcctca aaatgaccaa
    25141 acgattgcag cagctattta tcttcatagt ttcattggag accagctggc aaaagaacaa
    25201 tatattgttt tgccgacaca aatcagcgaa gcacttccga aatggatgaa ttatttttgt
    25261 aaaaaatcaa tggaaaacga ggcatgagtc atggagaaat ttacaggtta tgtgcgagaa
    25321 cctttttatt atgaaacaga tcaaatgggg attattcatc acagcaatta tatccgctgg
    25381 tttgaagagg ctcgggtagc cttattggta catctagggt ttccctataa aaaaatcgaa
    25441 gaatctggga tcatcatccc cgttctggaa gtaaattgca gctataaaga aatgatccat
    25501 tacggagaaa aagtgagcat ccatccaact atccaaaagt atacggggac acggttggat
    25561 tttagctacg aaattgtggg aactgaagac aaaaaattaa aaactactgg cagtagtaaa
    25621 cactgttttt tactttctga ttcgcaacga ttagtgaagc tgagcaaagt caatccggag
    25681 ttagatcagt tattccaagc gtacgcttct ttggatgatg taaattctta ataaagaccg
    25741 tctaagcatt tttcttgtat tgtgtcgagt agttcgtata atgaaaagaa aaaagcaggt
    25801 gatccccatg tatgtcgtta aagtaatgca cggttacatc gataaaactg gctgtcgcac
    25861 tcgggaaaaa aacttagaca atctgctcat tttcaaagac aaaaaagaat ccgaagcttt
    25921 tgctaaaaga atcggcggtc gtgtcaaacc gatccaagag gtacgaccag attgattcaa
    25981 tatttttctt ttcgaggctc taataggcat gcctatcagg gctttttttg cagactgtgt
    26041 ttttatgccc acacgatttg taagcgattt atgttataat agattatatc tgcatagaaa
    26101 ggaaggttaa atggttatca ataatttgca acagcaagct tatgaatcta tacgtaaaca
    26161 aatcatttat tccgagttag cacctggaag taaaatctcc gataaagtgt tagaagaaca
    26221 actagctatc gggcgtacac cgattcgaga agcactgatc caactgcgca atcaagagct
    26281 tgtaaaaacc attccacagt ccggcactta tgtttcaaaa atcgatatcc gatctgcttc
    26341 attagcccgt tatacgcggg aaaagttgga aaaaccgatc ctgcaagaat gcagtgcgaa
    26401 aatgaccaag caagatcaag ctaaactaga aaaaatacta gaacaaaccg atcaggcagt
    26461 aatcgctcaa aataaaaaag cttttttgat ttggatactg attttcaccg aacttgttat
    26521 gaaattgctg gaaaacgaga aatttgggat tggctagaaa gttatagtac gcacttgaac
    26581 cgtttcagat ggttacgttt gacgatttca gaattagact ggggtcgagt attagatgaa
    26641 catcagacaa tgcttcaatc aatgattgat cataactttg atgaagtcgg atttctttgc
    26701 acgatgcatt tacatatgat tatcgaggag caagaatacg tgattcataa ttaccccgat
    26761 tattttgaag atattcagga cagacccatc aaataagagg ttttggaatc agcgttttga
    26821 cctgagtatt agagaaagaa acggcaaaat agttcctcat attttgccgt tttttttgag
    26881 ctaataccga gggtcagctg attgcacacc gtttattgag aggttgtgaa aaagctgccc
    26941 tgcatcaagt aataagaaca gccaagtaaa aatagcttct tatgttttta acttggctgg
    27001 acttattacc gcagatgcaa gcttttgaac accgttttcc aagaggtcgt gagcctgccg
    27061 ttcatctccg agtcacaagg aacaatttca aaaaacagct tctcatgttt gttgaatatt
    27121 ttgacttgtg accgaggagt tgacaggaga acatcgttta ttcggaaaaa aaggactgtg
    27181 acacgacttt tgtcacagtc tctttatttg taatctaaat catactccgt acatcttttt
    27241 tctatttttt ttatttcttc taagatccct tggctacgaa tttcattata tggccaaagt
    27301 aagactgcct ctaatacata aggcgaaaaa tcgctttgat tcatctcttt tgaaaaactc
    27361 atcgctgcta tctcgctcaa tgcacgaatg tttgattgat gtgtataaaa aggcaaccgc
    27421 cacagcacaa ttttttcgct ttgcgtataa attcccttaa cagaagcttc gatgtggtga
    27481 aatagttcat gtccaataat caaagcttgc gctgtttctt tagagatcga agctagtcct
    27541 gaacatttta atttttctaa cggctccgtc atcaatcgaa tcccttcatc ttctgcataa
    27601 gttgcaaaag tgattcgttc gtttatttgc tctgattcac aggaaacgat tggaacagcc
    27661 atttgttcac aaatttctcc tacatcggca aaagggtact tttttttgat gctacatgcc
    27721 gtttcgatac cacaatcgat tgattgttgt ataagaattt tctgcttgga caaaggaatc
    27781 tttttccaaa ataaatctcg ccgaaactgg tagaatcccc aaatttcttc ggggatctgt
    27841 tgtaattcgt ttagtaactc ttctaactta tgtttgtttg atatttccgg caacgacgat
    27901 cacttcttcc tccggttcca tagtagatac ttccagatcc atcaaaagct gtagttgttc
    27961 cagatccgtc gctgtaaaat cagagactct tgctcctaag cagagataaa attctggtcg
    28021 ggcaatcaat tcagtttgat aaactgccat atcttcccaa agttgttcga cagctgtcat
    28081 ataattggct gcagttgttt tcatacatga agcatgtcca cgttggacct tgataaatcc
    28141 tttttgatcg atgatccgaa gacttccagc attttttgaa cgattctgtt cgccgcatac
    28201 ataaaagaaa ggtgtatttt ccaataagca aacttcattt ttatttaatc gcatatcctc
    28261 tgcagcaagc tctagtgctt cttctgttgt gatctccttg gttagatcgg ttgctttcac
    28321 ttccgtagaa ccagtagcga tagccgttac ttttgaagtc tgtggatcga tttctacgtg
    28381 gacttcgatc gattctggtg tagcgcctga ttcaatcgct ttattcatcg cttcattttt
    28441 caacgatcgt atatcttctt tacttggcga agggatgata cgttctacaa catctcggac
    28501 cattgcaagt gctacaccaa tagaagaaat cacttcggcg ttttcaggaa tcgaatattt
    28561 cacacccatt ttattactaa agtaagtaat caatgaggca gcgccgcccc ctacacctac
    28621 aagagaaatc tgatctttct ccaagtgata cttgtctgcc aattctaaaa tcaccggttc
    28681 aatcttagca taggattttt ccatgatctg ttctgcgatg tcttcaaccg tcgtatgaca
    28741 ataatcagct aatgcttgga tggcttttcg tgcagatggg acatttccat aagaaaaatg
    28801 ttcttcttgc actaatccaa ggacattggc tgcacatgta tttgtaatag tcacttcttc
    28861 tccgtcttcc atcactacct tgacgtaatc agcagggtct ccttctttag gagagaaaaa
    28921 ttcaacctta ggacctttga ttttttctgt ctccgtaaat actgcataat ctagaccggc
    28981 aatatgtgca gaacgtggac cgacatcgat gattcctgac tgattagccc gtaccatcga
    29041 accgcctgcc acacctagaa cccgaacatc taatgaagag atatatgttg ggtggccacc
    29101 tacaattgaa taatcgattg ctggacgtcc atttttaatg accccgatat tggttgttgt
    29161 tccgcctact tcaaaataaa caccattaga cgctctcaag tacatcaaag agcccatgac
    29221 tgatgctgct ggaccggaaa gcatggttaa aacaggtcgt tttttcattt cattgatttc
    29281 cattactccg ccgtctccgc gcataatcat caaagaaaca ttgacccctg cttctcgtac
    29341 ggaatcttct gtagaggttg ccgtatctag catttttggt aaaatagatg cattgattgc
    29401 tgctgtccga gttcttcttg tcaatccata taattttgtg atgtcagaag ccatcgttgt
    29461 agggatcgat tttaccgatg ctgcttcata aacaactcgt tctggttccc cattatctac
    29521 tccaaatgcc atagaagaaa ccaagacctc cgctctttct ctttctaaag aagaaatcgt
    29581 ttctgaaaca cggtctacat ttaagtgttt gacaggtaag aatgcattga caatctctat
    29641 ttttttctta ttccctaaat cgatatcgtt caatctagtt tgtcgttttg ctaaaaaacc
    29701 ttctaatcca ccttttgcca tcccgattac gccgactttt gctacgtctc cttcaatcag
    29761 tgcattagtc gcttgtgtgg tagaatgtgc aacaaacaca acatcttctg gactgatatt
    29821 attttccagc aaacaatttt gaaaagactg aacgacacct gcggctactc cacggacatc
    29881 atcgtgggtt gttttcactg aagattttcc gatgatttcg tgcgtcgcat tatcgattgc
    29941 gactgctttc gtatgtgtac cgccaacatc aattcccatt cgcacaaaac gtttcatttt
    30001 cttttcctcc attcatctaa tcgtttttat cgacgttttg tttactaggc gttcccgtac
    30061 aataagaacg tgatcaggca taagatcgct ccgatgatcc aaccagttgg gatggatttc
    30121 ttcatatatt ctcgagaagt tacttttgta taacctaatc cccaagcaac ccaagattgt
    30181 gtaatatcta aatgctgagg agcaatcgtt gtgattgcaa ataacgggaa caaaaaggct
    30241 ggcgacatcg ttggattaac agctaatacg acagctaaaa tagctgagcc tgaaccgact
    30301 agattcattg gaccgcggaa aaatcctagt ggtgtcaata tagcaaatac gattgccaag
    30361 actaatggtg attgcggtat ccaatctcca aaaatagctg aaaaataagg agaagcatac
    30421 gcagctgaat tattgaacat cgctaaggtc atcaagaaac caaccattgg cgcaacatct
    30481 actgcgccat ctgtaaataa tttcgcaaac tgacgacaga tttctttata acctccgcgc
    30541 aattttccag taaacagcaa tgcccagata cctgctagaa taaaacctaa gatgattgga
    30601 agatctaaaa gaacgacccc taaaactggt aataatacgg ctaaccatga gatcattggt
    30661 gcgtcgccac cttcagctgg tacgtttgct gccattgcat agcgtttctt tttgttcatt
    30721 gatatattag cgaccgttag taccacaact aagcttacaa tcatcccaat cataccaatt
    30781 tggaaatagt cattataagt atagctttca gccgctggat tgaaaccagc ataaatcgtt
    30841 tgatattgtt tgaaattgac gatattagca aagattccag ccataatcga gcccataaat
    30901 gagaacagtg caaccggtac tgaaatacct aatgacatca agattggcaa cacaataact
    30961 gcaatagaaa tcactggtcc gattcctgtc atcgacatga atataacagc ggttacaata
    31021 catagtaagc tcatcgtgat tcttggcttg tcccctccaa gttcaactac ctttcgaatc
    31081 agggtagctg cgattccgct atctactaga acacgcccga aaaaggcacc aaagaagaca
    31141 tttaccaaga tcgatttggc gtactcagct ggccccgtcg tatagatatg cgtcaacaca
    31201 tcaattactc cttgcccttc catcgccggg tttggtgaaa acgtgtttcc gactagcgct
    31261 aatcccatcc atacagtagc catgatggca aagccgacca tcaagttgta gccttttaca
    31321 cagtaccaga ccatgaaaaa gaatgagata actaacaaaa taccaataat aatatcactc
    31381 atgttattcc tccgtccatt ttgtttttct gataagcaaa ctaatatatc agtcaaactc
    31441 attctaactt atgtagttag cgctttcatt aaagggtgac cataaatgtt cagttttcaa
    31501 ttaaaaaacc ttaattatgc tattttagac ttatacgtta ttgtcctgtt tcatttgatg
    31561 gaggattgta aataaaaaaa gaggaaagaa cataactttt cgttatcttc tttcctcttt
    31621 ataattgttt agcttattta gccaaagctt cttttgcttg gtcagccaat gcagtaaatg
    31681 ctgctgcatc gttaacagcc aagtcagcta acattttacg gttgatgtcg atttcagcaa
    31741 ctttcaaacc gtgcattaat tttgagtagc tcaagccatt catacgagct gctgcgttga
    31801 tacgagcaat ccacaattta cggaagtcgc gtttcttttg acgacgatcg cgatatgcgt
    31861 agttgtatga attcattact tgttcttttg ctgttttaaa taaagtgtgt tttgaaccgt
    31921 agtaaccttt tgctaatttt agcactcttt tacgacgttt acgagttact gttccacctt
    31981 taacacgtgc catgtataat tcctcctaat aaaaataaat cttcagattc tgttatttgt
    32041 acttcttatt tcatttttgc tagttgttgg cgaatacgtt tgaagtcgcc actagaaacc
    32101 atgcttgctt tacgcaattg acggcgttgt ttttttgttt tgccgtggaa acggtgactt
    32161 gtaaacgcgc ggaagcgttt caatccgcct ttaccagtac gtttgaaacg ttttgctgat
    32221 ccgcggtgtg ttttttgttt tggcatgact atatttcctc ctcaaaatct ttcgttatcc
    32281 gactatcggc tgtccaacag ccagttagtc attcaaatta cttactgtcg tttttcggtg
    32341 ccagcgtcaa gaacatgctg cgtccgtcca ttttcgcttt ttgttccact gttgcaatat
    32401 ccgcagtttc ttcagctaag cgattcaaga ctttttgacc aatttcttta tgggtaatgg
    32461 cacggccttt gaaacggata gaagctttca ctttatcgcc cttttccaag aacttacgtg
    32521 cattacgaag ttttgtattg aagtcattca agtcgatcgt aggacttaag cgaacttctt
    32581 ttacattgat cactttttgt tttttacgcg cttcgcggtc tttcttttgt tgttcgaaac
    32641 ggtatttacc gtagtccatg attcgcgcaa ctggtggttt cgcaccaggg gcaactaaca
    32701 caagatccaa gtttgcttgt tctgcaatat tcaatgcttc tgcttttgtc ttaactccta
    32761 attgctcacc atctgaaccg atcaatcgta actctcgtgc acgaatgccg tcgttaacca
    32821 tcatatcctt tgctatggtc attcacctcc aaatattatg agagaaagaa aagcttaccg
    32881 aataaacggc aataaaaaaa cgagttctct tacagaaccc gcgcgtactt cacctaatag
    32941 aaccagtgaa aactatctag cccagtgaca ttatcaacta aggcgagaag cgggtgcttc
    33001 tgcttttatt tctcaaccat catagtatag caaaaactaa tctttttgtc aatgtttttc
    33061 atacagtttg tcttcctttt ctgaaaatga ttagattagc agactttcta gcagtgattc
    33121 ccgcttcttt gctatactaa agaaaaacga ggtgaaccca catgaaaaaa atcgaaccta
    33181 ttccaccttc tcttcctctt cttacaaagg gagagtttta tttagaagat gaggagatct
    33241 tctctggtct tctgatcaac gggggtgacc aaagctatct cgatgccaaa aatatggtat
    33301 taagagagag tcatatccaa aaacttacga tgcaaaaaac agttttacgt cattttgaat
    33361 gcagtaatgt catttttgaa aaatgtgatt tttccaatct ggagtggatt ggtggcagtt
    33421 tccaccaagt cgtgtttcat caatgtaaat taacaggtac gaattttgcc gaaagttatc
    33481 ttcgagactg tactttcact gattgtattg cttcgatggc ttcctttagt agtacaaact
    33541 tgaaagctgt ctccttcgat cattgtcaat tagaagacag cgaattttat gaggtcactt
    33601 ggaaaaacct ttttttgtct gacaatcagt tgaccggttc gaactggttt cgaacttctc
    33661 tgaagggatt agactttacg actaatttct tcacagcaat cgctttatct caagattctt
    33721 tggcgggact aatcgtcaac caagagcaag cacttgtgat cgcccaagcc cttggtttgc
    33781 agatcaaaga ctagatcaac gaatccgccg ccacagtgca ataagcatcc aaatagataa
    33841 gaccaaactg atgatcaagg cgattcccca tccgaaaacg gaatgggtaa agggtaactg
    33901 gagattcatt ccgaagaatc ctgtcacaat agttggaacg gtcaagatca gcgaccagac
    33961 cgtcaaaaat ttcatcgtgt cattcaagtt gttattcagc aaattattat aagtgccaga
    34021 gagctggtct aaaatctgcg aagctaagtt cgtcatttca actgcttgtc gtgcttcgat
    34081 cagcgcatcg tccaactctt ctttttcctt ttctgacaac ttgcgataaa tagccaatgc
    34141 cttgatttgt tctaaaagta ctacattttg ttttgttcct gtaactaagt aaaccaaacc
    34201 gatctctaaa tctgaaagtt gcagcagatt cttgttcgtt gttttttctc ttaatttcag
    34261 attaagccgc tgacgttcac tattcacttc ctcgattaaa ggaaaaaagg aatcagaaat
    34321 caaaaacaat gtacgaaaca ataaactgta tacactcatg tctggcattc gttccaaaag
    34381 cgatcgaatt agtcgaatca cgtactcggt attcggcgtg gtaaatgtaa acaaaccgtc
    34441 ttcttttaaa ataaaagcga taggactagt ttcataatga ttatcgactt tttgctggtg
    34501 cggaacattt gatacaagca gtaacgcttc atcaaaagaa tcgtactcta cgcgcgcccg
    34561 ttcattttta tctagagaat aagtcagcat ctcgtcacta atgccatatt cttcttttaa
    34621 gtttttaaca gctagcacat cgttttgctg tacattgatc caaaaattac ggtcttgatt
    34681 aaaatagtgt ttttcccgca tactctatcc tcctgacatt tgtttccagt ataaagaaaa
    34741 aagtcgctca aataaaggtt taccctttat ttgagcaact gtaaatcaaa tggctacttt
    34801 ggtttggcga atctctgcta atttttcttc gatcattttc aagacttctt cttttgcttg
    34861 aggatcttcc acaaaattat agcggtctcc atcgatttgg atttttggac tttctttgta
    34921 ttcttcatac cattgatcgt aacgtctgtt caattctttg taataatcgt atagacttgg
    34981 atcaaaggac aattgttcat acgaacgacc acgtttttcg atacgctcca acatcgtatc
    35041 aaaagataca cgaatatgaa ctaataaatc cggatgtttt ttatgggcag catagggaag
    35101 ttcttccatc atgttttcca gcagttcatc atacacacga acttctgttt ctgttgctct
    35161 tcctagatct gcatttagat ggaaaagcag tgaatcttca taaatcgaac ggtccaatac
    35221 attattgtca tctttcattg cttgtttgat gcttgcaaaa cgtttattca aaaaataaat
    35281 ttgcagcaaa aaagcatact gttcagggtt tgcataaaat aagggcaaga cttcattatc
    35341 atcaatcgat tcataaaatg cctgactatc aaagtgttca gccatcattt ctgttaaact
    35401 tgattttccg gcaccaattg tacctgctaa aacgatcacg ctcatttacc tccagctcac
    35461 ttcatcacta aatattgtgt ttaactccaa cctttttaaa tataccccca atatctagta
    35521 gtgtcaattg tatttttttc tagtcaaaga agaaaaaaag tcaaaaaatt cttaagaaaa
    35581 aagtgttttt tctttagaaa acgtattctt agttttactt ctgagatggt tttagtaaaa
    35641 taaaggagga atgttttttg agacccttag tggacaaaaa aattcgtgaa gcagcgcaac
    35701 tcgttttggg cgctttattt tttgcttaaa aatcactttt acgtgagaag gagttagatt
    35761 atggcaaact atggccaagg agaatcaagc ggaaagatca tattgatggg cgagcacgcg
    35821 gttgtttatg gagaaccagc gattgccttt cctttctatg caacaaaagt caccgcattc
    35881 cttgaagagc tggatgcaat ggacgatcaa ctggtttctt cctactattc aggaaattta
    35941 gccgaagctc ctcatgcatt aaaaaatatc aaaaaattat tcattcactt aaaaaaacag
    36001 catgacatcc aaaaaaactt gcaactgacc attgaaagca cgattcctgc tgaacgtgga
    36061 atgggatcaa gcgctgcagt cgccacagca gtcactcgtg ctttttatga ttacttagca
    36121 tttcctttgt ctcgtgaaat actattagaa aatgtccagc tttcggaaaa aatcgcccat
    36181 ggtaatccta gtggaatcga tgcagccgct actagcagct tgcagccgat ttattttaca
    36241 aaagggcatc ctttcgacta cttttctttg aacatcgatg cttttttgat tgtcgctgat
    36301 acaggaatca aaggacaaac aagagaagcc gtcaaagatg tcgctcacct ctttgaaacc
    36361 cagcctcatg aaactggaca aatgatccaa aaattaggat acttgacgaa gcaagcaaaa
    36421 caagcaatca ttgaaaattc accagaaacg ttggcacaga caatggatga atcacaatca
    36481 cttctggaaa agctgacaat cagcaatgat tttcttaatc tattgatcca aacagcaaaa
    36541 gataccgggg cattgggtgc caaattaact ggcggtggac gcggtggctg tatgattgca
    36601 ttagcacaaa caaaaacaaa agcccaagaa atcagtcaag cacttgaaga tgcgggtgct
    36661 gctgaaactt ggatacaagg attaggagta catacctatg tttaaaggca aagcacgcgc
    36721 atataccaac attgctctaa tcaaatattg gggtaagaaa aatgaagaac tcatccttcc
    36781 aatgaacaac agcctttcgt taacattgga tgcattttat acagagacag aagtcatctt
    36841 ttctgatagc tatatggtag atgaatttta tctagatggc accttgcaag acgaaaaagc
    36901 aacaaaaaaa gtcagtcagt ttcttgacct ttttcgtaaa gaagccggcc tctcactaaa
    36961 agcttcagta atcagtcaga atttcgttcc taccgcagct ggattagcct cctctgccag
    37021 cgggctagct gctttagcag gcgcttgcaa tactgctctt aagcttggat tagatgatct
    37081 ctctctttca agatttgctc gacgcgggtc tggttcagct tgccgaagta ttttcggtgg
    37141 tttcgtcgaa tgggaaaaag gccatgacga cttaagttct tacgctaagc cagtcccttc
    37201 cgattctttc gaagacgatt tagcaatggt tttcgttttg atcaacgacc agaaaaaaga
    37261 agtgtccagc agaaatggga tgcgtcggac agtcgaaaca tccagttttt atcaaggctg
    37321 gttagattcc gttgaagggg atctatatca attgaaacaa gcaatcaaaa caaaagattt
    37381 ccaacttctc ggagaaacga tggaaagaaa cggactaaaa atgcatggaa cgactttagc
    37441 tgcccagcct ccattcactt actggtctcc tgacagctta aaagcgatgg atgcggtgcg
    37501 acaattgaga aagcaaggca ttccatgcta ctttacgatg gatgctggcc ctaatgtcaa
    37561 agttttagtg gaaaacagcc atttatctga ggtacaagaa acatttacca aattatttag
    37621 caaagaacaa gtaattactg cacatgctgg tccaggaatt gcgattattg aataaaggga
    37681 gatttttatg attgaagtat ctgcaccagg caaactctat attgccggag aatatgcagt
    37741 tgttgaaaca ggccatccag cagttatcgc tgcagtcgat caattcgtga cagtcactgt
    37801 agaatctgca cgaaaagtcg gaagtatcca atctgcacaa tatagtggga tgcctgtacg
    37861 ttggacaaga cgcaacggag aattagtttt agatattcgg gaaaatcctt ttcattatat
    37921 ccttgctgct attcgcttga ctgaaaagta tgcacaagaa aaaaacattc ttttatcctt
    37981 ttatgatctg aaagtaacaa gtgagttaga cagttcaaat ggccggaaat atggattggg
    38041 ttctagcggt gccgtgaccg ttgcaacggt caaagccttg aatgtttttt acgcgttgaa
    38101 tttatctcag ttggagattt tcaagattgc agcactagcc aatttagcag ttcaagataa
    38161 tggttcctgc ggcgacatcg ctgctagctg ttatggtggc tggatcgctt tctcaacctt
    38221 cgaccatcct tggctccaag aacaagaaac tcagcattct atcagtgagt tacttgccct
    38281 ggattggcca ggtctatcca ttgagccatt gattgctcct gaagatttac gtttattgat
    38341 tggttggacg ggtagccctg cctctacttc tgatttggtc gatcaagttc accgttcgag
    38401 agaagataaa atggtggctt atcagctttt cttaaaaaat agtacagaat gtgtcaacga
    38461 aatgatcaaa gggtttaaag aaaataatgt aacgttgatc caacagatga ttcgaaaaaa
    38521 ccgacaatta ctgcatgatt tatctgcaat cactggggtc gtcatcgaaa cgcctgcttt
    38581 gaacaaattg tgtaatttag ctgaacagta tgaaggagcc gcaaaatctt ctggtgcagg
    38641 tgggggcgat tgcggaatcg taattgttga ccagaaatct ggcattcttc ctttaatgag
    38701 tgcatgggaa aaagcagaaa tcactccact gccgttacat gtctatagcg atcaaagaaa
    38761 ggaaaaccga tgaatcgaaa agacgaacat gtttcactag ctaaagcctt tcatgataaa
    38821 caaaaaaacg aatttgactt cgtacggatc atccataacc ctttgccgca aatagcagtt
    38881 tctgatgtcg atctaagtac acaggcagtt gggtttacac taagcagccc attttatatc
    38941 aatgcgatga ctggcggaag cgaaaaaaca aaaaaaatca accaagatct tgcaatcgtc
    39001 gcaagagaag cagatttgat gattgccact ggttcagtca gcgctgcctt gaaagtccct
    39061 tctttagccg atacttatac gattatgcga caagaatatc ctcacgggaa aatcattgcc
    39121 aacatcggtg cagggacttc tgtcgaaaga gccaaagaag ctatccgatt gtttcacgca
    39181 gatgccttac agattcattt gaatgcacct caagagttgg ttatgcctga gggagaccgt
    39241 gattttacca attggaaagt acttattcaa gaaactcaaa cagccattga cgtccccctc
    39301 atcgtaaaag aagtcggttt tggcatgaca agagaaacgc tcaatgatct agctgcttta
    39361 ggagttcata cagttgatat cagcggtcga agcggaacaa gcttcaccca gatcgaaaac
    39421 gcccgtcgct ccaagcggga actgagttat ttagctgact gggggcaatc gacagtctct
    39481 tcattgcttg aagcaaatga agcagacact tctatggaaa tcctagcctc cggaggtatc
    39541 cgcaatgcct atgacatatt taaagccctt tgtctcggcg caaatgccgt gggtacttca
    39601 ggaaccgtac tgacgcatct gatgaatcac ggcgtagaag aaactattat actgatgaag
    39661 caatggcaag aagagcttcg tctgctttat actatggtcg gcgcaacgaa taccgcggcc
    39721 ctacaccaac aatctctgat cttttccgga ccagtgaagg attggtgcga agcaagaggg
    39781 atcgacttag tgaagtatgg aagacgcaca gaaaagagcg tgggacaaaa gtaaagaaca
    39841 cttttgtctc acgctctaaa cacgtacaaa cggcggaagt aaaagcaact ccttcggaaa
    39901 taagctgaaa ttcacaaaaa tttgaaaagc aattttcgtg aattccttct tatttcttgg
    39961 agttaaatgc ttctgtccca gcctcttttt tcgaataaat gatgtttaat cagctgtcct
    40021 tcggtattcg ttcaatccgc tgatttcaca acctctccac taaacagtgt actaaagctc
    40081 ttttaaggtt gttttccgat ataggcaagt atgcctccgt ccacatacaa gacatggccg
    40141 ttaacgaaat cagatgcgct agaagccaag aagacagatg gtccagccaa atcgattggc
    40201 tcgccccagc gtgctgctgg cgtacgtcca acgataaatt gatcgaatgg atgacgttca
    40261 ccgttttctt gtgtttcgcg taatggagca gtttgcggtg tagcaatata acctggtcca
    40321 attccattac attggatatt gtattgaccg tattcagaag cgatgttttt cgtcagcatt
    40381 tttaatccgc cttttgccgc agcataagca ctgaccgttt cgcgtcctaa ttcactcatc
    40441 atggagcaga tattgatgat tttaccgccg cctttttcga tcatatccgg aataactgct
    40501 tttgaaacga tgaatggggc atttaaatcc acgtcgatta cttggcggaa gtctgccgca
    40561 tccatttcga tcatcggtgt ccgtttgatg atccctgcat tgttgactag aatatcaact
    40621 gaacctacat cttctttgat ctgttgaacc atcgcttgaa ccgcttcttc atcggtcaca
    40681 tcgcagacat aaccttttgc atcgatgcct gcttctttat aatgcgctat accttcatca
    40741 actgattctt gattcaagtt attaaatacg atttttgctc ctgctgtagc taaagagcgt
    40801 gcaatctcaa atccaatacc atagacagca ccggtgacta atgctacttt tccatctaaa
    40861 cgaaacatat ccatactaaa ttccatcatt ttctctccct tatgcttatt ttaattgatc
    40921 catttgcacc atatccatgt ctgtataggt gatattttct ccgcacattg cccagatgaa
    40981 tgagtaattg cttgtaccta cacctgaatg gatcgaccag cttggcgaaa taatggcttg
    41041 ttcattgtct acaactaaat gttttgtttc atctggtttg cccatcatgt ggaacacacg
    41101 tgtatcttca ttggcataat caaagtagac ataagcttcc atacgacgtt catgtgtatg
    41161 acaaggcatc gtattccaag cgcttcctgg ttctaaaatc gtataaccca tttgtaattg
    41221 acagctttcg catacatttg gatggatgta ttggtagatt ttacgttcat tcagtgtcag
    41281 gccttcacca gtctccattg gttttacttg gtcgatactg atttttacat ttggatattt
    41341 atgatgagct ggaacagaac taatatagaa tttagctggg ttttcagcat cttgagaaga
    41401 aaatacaaca tgtcgtgttt cttttccgat atagtatccg tcttgttttt tcatttcttc
    41461 tttttgccca tcgatctcga tgaatcctgg tccaccgata ttgatcacac ctagttcacg
    41521 acgctctaag aagtaatcaa cacctagttc tttgtctaat gtgatttcta attctttttc
    41581 agtaggtgtc actccaccaa agatcattcg atcgttgtgc gtataagtca aactgatttt
    41641 ccctggttca aatacctttt ctactaaaaa ttcttttctc aattgttccg ttgaataatg
    41701 acggatatct tctggactgt gtgtataacg tgtttccatt tcttgcattt tcttctactc
    41761 cttctatcga atgatttttc cagagccgca tgctaaaaat tgtgcgactt cttcttgact
    41821 aaattggttg cagtccccat ggatcgtatg cttcaaggca gaagctgccg tagcaaaatc
    41881 aatgatttct tgcggagaca tattggataa aatcccatgg agcacgccac ctgcaaaagc
    41941 atctccgcca cctactcgat ccacgattgg ggtgattcta tggacttggg attccacata
    42001 ttcatggttc atccacaatg tccccttcaa ttcattttca cttgctgaat gaacggtacg
    42061 cttggtcgaa taaaacacct gaatattcgg gaacttttcc tgcatttttt gataataata
    42121 aattagaggt tgttcttctg cttctggcgc tttctcgata cctaacaaat aaagagcatc
    42181 catttttcca gctgagcaga tatctactaa aggtaaaagc tgatggatta cttcgctggc
    42241 ttcttgctgt gtccataatt ttccacgata gttgatatcg aagctgatca ggcagccagc
    42301 tttttttgca gcttctatca cctgtttcgt cattttttgc cattttttag acaaagctgg
    42361 ggtgattcct gaaacatgaa atatatctat tccttggaat aactcctcta gcgaccattc
    42421 caaatgatcc atttcagcaa aacttgatcc ggcacgatca tagatcactg aggcggcgcg
    42481 ttcgccacta ccagtttcca tataataagt acctagtctt gatccaccag ataatacctg
    42541 tttcgtatct actccataac attgcaagtg ctttttgact gcttttccca atgcgttatc
    42601 aggtactttt gtagcaaaaa tcgcttcatg tccgtaatta gctaaagaaa ttgctacgtt
    42661 tgcttcccct ccgccataat gggcagcgaa tcgttcactt gaatcgatac gggttccctg
    42721 ctctgtggac aatcgcaaca tgatttctcc gagtgtcact acttttccca taagcagcta
    42781 cctcttgatt tctttcattt ttgctgcata ctgttgcgcg acttccgtta ctttgtcaaa
    42841 atctcctgta gctgcgggtg ctaaaaggtt cccacctact ccgactgtta cgacaccagc
    42901 atcgaaccac tctttcatat tgtccagact cacaccgcca gttggcatga tattgacttg
    42961 aggcatcggc gccttgaagg cagaaatgat acttggtcca taagcgcttc caggaaatag
    43021 tttgacgata tctaccccgc ttttcagcgc tgttttgatc tcggtaatag tcatacatcc
    43081 tggtaagtaa ggaatttgat acagattgca catctctgcc gtttcttgat caaaggaagg
    43141 gctaacaata tactctgcgc cagctaagat agccaagcga gctgtgacag cgtctaatac
    43201 tgtgcctgcg ccaatcacta tttctggttg gtctttgtag aatgacaaaa gttctttgat
    43261 tacttgatct gcttgaggga ctgtaaaagt caactcaata ccagttagac cacctttgat
    43321 gattgcgtgg caagcattca aagcttcttc tttgcttttt ccgcgtacca ccgcaatcac
    43381 tccggcgttt ttcaatcgag ataaaatatc aatttttttc atcttctcca cctatttcct
    43441 atttagaaat tttattttat aacttggaaa tctataccca tattatacgg cagaaaagaa
    43501 aaattgcaaa cgcttttttc attatttttg aattctttgc tcgatttcgc tttttgtttg
    43561 cttgagggca ctgattattt gttcttttgt ctcatctgtt aaacgatatt tcggcatact
    43621 tacactaaat gcaccaatga tttgttcgtt tttcacgatt gaggctccta tacaaaaaat
    43681 atcacgttcg atttcttcat cgtcaaatgc gatgtgggtt tcttttaccg ttttgatttc
    43741 ctttttcaac cgtaatgcat tggtgatcgt atattctgtg taagggatca acggatgctt
    43801 ttcaagataa tcagtgtact gctcttcact gaattctgct aatacggctt tgcccatcgc
    43861 agaattgtat aatggtcggg tgattccgat ttttgaagac atgcggatcg tttgatgttt
    43921 tggatctagt ttgttcacgt acaagatctc attgttgtcc aataccccta agtggatagt
    43981 ctcatcgatc ttttgctgca gctgttctaa aaacggtaaa gtcaactcta ccaaatcgat
    44041 ttgttcaatg tttttattcg cgtagcgaat aagctttgca cctaaacgat agtttttctc
    44101 tgaattttta tttacgtagc cgatcatcaa caaagtatct agtattttca agactgtaga
    44161 agaggtcatt cccacaccct ttgcgatcgt ctgcaaagag acatcttggt tttccgctaa
    44221 atagtctagt attttagagg ctttgatcaa tacagttcca taaggctttt tattttccat
    44281 ataatattct cctatttctt tataagaaaa attatagcgg aaataccatc aatttaaaag
    44341 agatatccag atataaacta ccatttctct ttacaggcaa tttaatgttg aagatccgct
    44401 tcgtttttac atcaaacttt gatataaagc aatgatttct tctactgtcg tttctcttgg
    44461 atttcctggt gtgcatacat ctcgcaaagc atcttctgca attgctggaa tatctgcttc
    44521 ttttacacct aattcagaaa tggtttctgg gattccaacc gctttgctca accgatcgat
    44581 ctcaccacaa gccgcttctc ttacatcttc taaagacatt tcagctgctc ctttaatccc
    44641 tagaacaaga gcgatctcac ggtacttttc tccagtatat tctttgttat atttcatgac
    44701 agttggcaaa agtgctgcac acgctacgcc atgaggaatg ttgtaccatg cacttaacgg
    44761 gtgagccatc ccgtgtacca gtcctaaacc gacatttgag aagcccattc ccgcaatata
    44821 ctgtcctaat gccatttttt ctcgcccatt ttgatcgcca tctacagaag cttccaaaga
    44881 atggccgatg atttcgattg cttttaaatg cagcatatca gtcatttccc aagcgccttt
    44941 tgtgataaac ccttcaatag catgcgtcat agcatccata cctgttgatg cagccaattt
    45001 tttcggcatt cccatcatca tatcgctgtc gatgaacgcg acgattggga tatcatgagg
    45061 atcaacacaa acaaattttc ggtgatttac ttcatctgtg atgacgtagt tgatcgttac
    45121 ttctgctgct gttccagaag tcgttggtat agctaatata ggtaaacatg gatttttcgt
    45181 atcagcaaca ccttctaaac taatgacgtc tgaaaactct ggattcgtca aaataatccc
    45241 aatggctttt gccgtatcga ttggcgaacc tccaccgatt gcgatcaaac agtctgcctc
    45301 tgcctcttta acaaaagcca ctccatcttt tacattttgg atcgttggat taggtacgat
    45361 cccgtcgtat aaagcataag cgattgctgc tttatctaga agttctgtta cttttgttgc
    45421 tataccgtgc tcgatcaatc ctttgtcagt aatgacagct gctttgccga atcctcgctg
    45481 ctgaaattca gggacgatcg actcaatcgc tccttttcca aaatacgatg tttcatttaa
    45541 aatcattcga ttactcattt tctattcctc ctaatttgta ttatttttca caataaaaac
    45601 gaaagaaccc caagcaaccg gttgcccagt tgcttgttgc tacagttcaa agactggcat
    45661 caattcccaa ctgactggag aggcatctgg atttgtctcc atgatatctt ccatgtactt
    45721 ccaccatttt tgattgatag atgtatttgc cattttttcc cataactttt catcttcgat
    45781 cactaagtaa ccaaataaca aacttgtact cggatcaaga tagatcgaat agtcgatgac
    45841 cccgtgttca cgaagtgctg tacgcatttc tggccaaagt tcatcatgtc tttttttgta
    45901 ttcttcttgt ttttctgggt aaactttcat gcaaaaagct tttttcacca tgtcagacac
    45961 ctgctttcgt atgaacttcc tccaaataac ctgcttttgg agtgacacca aacgcatctg
    46021 ctaaatgcca caggttttcg tccgtgattg tttgcaagat tggtccttgc gctttgacgt
    46081 acgtatagac ttctgctgcc ttttctgctg tctcgatcaa accaaagact tcatccatgt
    46141 cttttcctga cccgtagatt ccatgttgtg gccataaaac caatcgagtt tctttcattt
    46201 tttcagctgt tgcttcccca atctcatttg ttcccggaac catccacgga atgatggaaa
    46261 ttccttctgg aaaaacaact aagcattccg tacacatttg ccatagcgta cgactaaagg
    46321 aacgttcgtc taattcatgc gtaaaactca tcgctaatag atgactagca tggcaatgca
    46381 taacgatccg attatctggg tctacttcta aacgggcgat atgactcatg aaatgacttg
    46441 gcaactcgct tgtaggaagg cattcgtttt ccagccccca caaaagttcc agtgttttgc
    46501 catcttcagc aactcgaacg attccaagat tctcggcagg ttcgctcgct acatttttaa
    46561 aatatcttcc tgaaccagtt acaataaaat agcatcctgc taaacggctg gcatcaaaaa
    46621 tcatcgagat gcggcggatc actttttcag gatccaagaa cggtgtgatc tcttcttctt
    46681 ttagtaaata agaaatattc ccgccatttc tttcatccca tcccaagcga taaagattcg
    46741 acgtcgtttc gatcatttct ttcacataag gcgcttgtaa aatatctatt tttttcaata
    46801 tagccacact cctattttct taattccaac acgtcttttt gatattcctg gacttcttcc
    46861 agccaatcca aaccaactgg aacttgattt ttgtaacaga aataattcca tacatcggca
    46921 tacgggaaat ctttcaattc ttccgtataa gccattcttg aagtaaagtc aaaagccagc
    46981 tccatttctt tcaatcgctc aactggttcc agcatagctt tcagcagtgc tttttgtgta
    47041 tttctcgtac cgatgaccca agcagcgata cggttgatcg ttgcatcaaa gaaatccaga
    47101 ccgatatttg ttttttccaa caattcgtta cggactaatt ctttagcgat ttcctgcaat
    47161 tcatcatcca tcaaaaccac atggtctgaa tcccaacgca ctggacggga aacatgcaac
    47221 atgattcctt tgctgaataa agctaatgaa gataatttat tggaaatcac ttctgttgga
    47281 tgaaaatgtc cagcatctag acaaacaagt ttgtcacgag tcaacccgta acccatataa
    47341 aattcatggg aaccgactgt ataagcttca gcgcctaaac caaatagttt gctttctact
    47401 gcatcttgta cataccgttc atctaactct tctgtaaaaa tttcatcgag tgattccatt
    47461 aaacgttccc gtggggtaaa acgatcaatc ggattatctt tcatcccatc aggtacccaa
    47521 aagttattga tacttgtttg cccagtttct ttcccaaagt actccgcgat tttgcggctt
    47581 cttttcccgt gttcgatcca gtattcacga acttctttat ctggatgagc caatgtaaag
    47641 ccgtctttca tcattggatg cgaaaagaaa gtcggattga agtcaagacc gatttcttct
    47701 ttttttgccc actctaccca tttttcaaaa tgtttcggct caatttcatt tagatcgaca
    47761 gattcttctg tatccaaata gatagcatga aggttcaatt tgtgtttgcc cggtatcaac
    47821 gaataagcct tctccagatc ttgtctaagc tcttcaggtg ttcgtgcggc tcctggaaaa
    47881 ttccctgtcg ccatgattcc accggtcaat tcgccatccg gtgtcaagaa acctttgaca
    47941 tcatctcctt gccagcaatg catagaaatt ttgatgtcct gtatcttttc tagtacagca
    48001 tctgtatcta caccgatagt tgcatatttt tcttttgctt gttcgtaatt tctttcaatc
    48061 gtgttcatac gattactcct ttgattttat tcacgtacca ttccatcgtg ttttgcttat
    48121 tttcatctgg gtaaaaagtt tcacatggaa acgactgttt gattacattt cttgcttgtt
    48181 ctatattcga aaactgacct gtactgagca tttgcatggc aacattcccg attgctgtgg
    48241 cttcactagg tcctgcttct acaatgattc cggcataatc tgctgtcaat tgattcagta
    48301 gacgattatt cgatccgccc cctacgatgt gcagcttttc caatttcgtt ccagttaagt
    48361 gttctacttt gatcagctct tgtgcgtaac aaagagcaag attatcatac acacaacgag
    48421 cgatagctcc cgatgagaca gggactggct gctctgtttc aaaacagtat tgctgaatct
    48481 cttctatcat atttggggga ttcaaaaatc gagggtcatt gatttccatc acttgctgca
    48541 gcggtttttc ttttgcagca agctctgcca attccgcata actgtactcg tactcttgca
    48601 ttcgggcaac ttcctggatc aaccacatcc ccatgatgtt tttcaaaaaa cgcaccgtgt
    48661 tatgggcacc ccattcattc gtataatttt cagaaaaagc attgcgagaa acattggcga
    48721 tctttgtttc aatccctaaa agcgaccatg tcccactgct gatataaccc caacgttctc
    48781 cttgacctgg cgttcctaat acagctgacg ccgtatcatg acttgctact gtgataaagg
    48841 tcacatctgg caaatcatat tttgaaaatt gttctttttg cagatctcct agtatcgttc
    48901 ccggctctgt caatggtgga aataattcct tcgagacacc gattttttca agaagttcta
    48961 tttcccattc ttttgtccct gcattcagca gctgcatagt agatgcatta gttttctccg
    49021 tcaccatgtt accagtaaag acatacccta aataatcagg tatcagtaac aatttatgtg
    49081 cttttgccaa attttctttt tcttcagcaa acaattggaa caaggtattg aaaggctgga
    49141 tctgaatccc tgtctgctca tacaacttat ctagagaata gatttcagaa aactgttcta
    49201 ctgctccatt ggtacgcttg tcccgataag caatcggatc atttatccga tccccttgtt
    49261 catcaatcag acagtaatcg actgcccatg tatcgatccc cgcaaaaagg cgatcaatcc
    49321 ctgtctgctt taatttttca aatccaatca aaatctcttt gataagataa tcgatcttcc
    49381 agcgatcgtg accgtctttg ttccgaaagc cgtttttgaa ccggtgtact tcttccaaga
    49441 tcaaagaacc ttttgcatcc ctcgtacttt tcattagtcg tccgcttgat gcaccgatat
    49501 cgatggcaac aaatgttgga ttcatttctc cacctctttt actttattgt tgattagcac
    49561 tttattaata aagcgttatc atttattgac aatgctatca taatacagaa tcttatattt
    49621 tgcttcacac tattttggta tataattgca ttatcttgac aagttgggag tgtttacatg
    49681 gattactggg aaaaagccaa ccactcatgg tcggaagact caaaaagagt gatcttgacg
    49741 cctacacaaa gaagcaggaa tttatttttt tatatccaag aaatcggtta cttcaaagca
    49801 ccaatccctt actatactga acgagcacat ttaccttctt acttgataaa gtttactttg
    49861 tctggcaaag gttcgcttac atataatgat aatcagcaac agctaaaagc tggagactta
    49921 ttttttattg actgccaaga atatcagcgt tatcaaacga tcagcgaaga accatgggaa
    49981 atggattgga tccatttgaa tggtcagaac gtccaatcat tttatcagga gttcacaaaa
    50041 gacggccata acaccttcca ttcaacagga gatccatcag aaaacaagat ccaccatttg
    50101 atcacagaat tgatccacat gcaacaaggc acgaatgccc gtaccgattt ccagtcctct
    50161 gtagctatcc acgagttgct gaatgaactg attttgcaaa agtatcagct agatttcgaa
    50221 gaagaggata ttcccgccta cgtttacaaa ctgaagcaat acttagacga acatttcaaa
    50281 gaaacgatta cacttgatca attagaacaa caattcttat tgaataaata tcagctgagc
    50341 aaagattttt ccaaatttat cggttctcca ccaatcgatt atttgatcag caaaaaactt
    50401 tcttacgcta aagaccttct tcggtataca gaatttacgg ttcgaatgat ttccgaagaa
    50461 atagggattg agaattttgc ttactttagc cgcctgttca aagcaaaaac aggactatcg
    50521 ccgagagact ttcgtaaatt cagctgattc atataatatg tacgtcattt tttcaaaata
    50581 ttttgaaata tatagataaa aataaaataa ccgcagtttc tgcttctatg taaacaacag
    50641 cagctactgc ggttattttt tagtgatcat tctcattgtt catcttcaaa atgttcataa
    50701 tgaaccaact gtttctgtgt atcacgtttc aaaaattctt taggcgttat gccgaactct
    50761 ctcttgaatg ctttcagaaa atgcggataa gaagaaaaac cacttttact atatacagca
    50821 ctagagctgc ggtattctcg caacaagtgc ttggcataga tcagcttttt cttcattaca
    50881 tactgatgaa acgtaaatcc agtgtacttt ttaaattctc gtgtgatatg gtatttgctg
    50941 acaaaaaact gcttttccat ttcctctaaa gaaagcggat gagataaatt ttttgagata
    51001 tattgtaaca tttgttccac acgctcatta ggaactacag cattctgttc ttgcgcactc
    51061 tcttcttttt gtacaacttg attgagaaaa atcaagtatt ctaccagagc ttgttcatat
    51121 tttatgtctt caccgtaaat ttttttatca ggaggatggt cgataaatga taatttttgc
    51181 ttcaatactt cctgttctat ttgcaataca cgactcctct tctctcccat tggttgaaaa
    51241 caagccgata gattagacca tggcgtggaa cgactttcta gaaaagaagg ggtgacaaag
    51301 atatagacac gctcgaatac atctgaagat tggcggacga tacgatgcaa atcattggaa
    51361 tggatgagca gcacacttcc tgctttgatc gtaaattgtc gtccgttcaa ataaaaagtc
    51421 gcttctccct ttaatgtagc atggatctca tagaaatcat gatgatgata gatcgtttta
    51481 ttttggctga cgacatcttt gacatgatag atttcatact gatcagaaac catcgtattg
    51541 acttgttcac cgctgatttt cttttggagc atgtgccgca cctccttttg ttcacttatg
    51601 cacaatttta ttataccacg ttttttgtaa tcaaaaagag ctgcttttat atactcttat
    51661 aaaaaagaag agacgtggca tcttctagcc ccgccccctc cattctaaat attagttaac
    51721 caacgtcttc tttacattat tcaagaagac ttcttccata acgaatccca tcatcttttg
    51781 tttcttcaag aaccacatac aggtacggac gattcttcat gatgatttct ttgatttttg
    51841 tgtactcgat cacaccgtct cccaaattta ccggcacgat tttttcctgt tcgacacgaa
    51901 aatctttcaa atgaaccgca catatctttt caccaaaacg ttcaaaagct tcttctacca
    51961 attggacttg ttcttgataa tcactacttg tgatcaaatt ggtcggatct aaaatgattc
    52021 ctagatagtc cgaatctact gcttggatca gctgctctac ccttgctaaa gaatacaccg
    52081 gatgattcaa acctggttct atccccacca tcatttggta tttttctcca gcttttacca
    52141 agcgacggat aacggacaca gcttcagcaa atgcttcatc tgtgaaattt tcttccgtat
    52201 attggatttc cggcaaaaca catccagttt ccgaagcaac catcgacgca ccaaaggaag
    52261 aagcatagcg aagatactgc tcgaactttg tcagcaactc ttgtcgtatc gttaaatctg
    52321 gatgaatcat attgatatag caacttaaaa ttcctacggt cacttcttgc ttttctaaaa
    52381 ctcgtttgac ataatttccc atcccagaat tgatctcatt tgcgccagaa ggtatttcag
    52441 gaaacgaaag acctaaggcg agctgcagtg tatgaatccc ttgttctttt gttttttctg
    52501 caagatcttc tatcgtttgg acttcggaaa gatcatgtcc tctaattgta atgttcaaca
    52561 taactattct cctaactcag catattgggt agccacatga cgatttgcgg gaagtaagta
    52621 atcaaaaaca agacgactaa gataacccca taaaaaggaa gaagcggttt cagcattgcc
    52681 tctgctttta cgcctcctac acttgcaccg acatatagtc ctgtacctac tggcggcgtg
    52741 atggagccta cacacagatt catgattgaa aacaacccgt aatgtacagg atcaacccct
    52801 acacttgtga cgattggtaa aaatattggt gtaaaaatca agatagcagg cgccacatcc
    52861 ataaacatac ccaccagtaa taacacaaga ttgactaata acagaataat aaatttatta
    52921 tccgttacgc ccataatcaa cgaactaatt gcctgaggaa taccggtaaa cgccatggcg
    52981 aaagacatca tggaagaagt agccaataag aacatgatcg tccctgacat tgctactgat
    53041 tgaaacaaca tattaggtat atcttttagt tttaccgttt tatagtaaaa catagcaatc
    53101 aacagggagt aaatgacagc tatcgctgaa gcttctactg ctgtaaatat ccccgtcaag
    53161 ataccaccga taatgatgac aatcaataga aggctaggaa tcgcctcttt tattattttt
    53221 ccagcatgag ccatctgtcc tttttcgaca acagggtatt tattcttttt agcaacgatg
    53281 aatgctacta gcatgattcc tagcgcccac aaagcgccta cgagataacc tcccataaac
    53341 aaagcagaga tcgaagcacc accagcaaca agtgaataca tgataaaagc agtacttgga
    53401 ggaatgacca tacctgtcgg agctgacgcg atattgaccg ctgtcgcaaa cttccggtca
    53461 tatccttctt ccacttgctg agggatcaac actccgccaa tcgcggtaga agcggcaatg
    53521 gccgagctag agatggaacc aaataaagca ttccctaaca cattcgtatg agccaaagcc
    53581 cccggcacgc gcccgacgaa caacatcgca aaatccacta gtttcttagc tatccctcca
    53641 ttattcatga taatgcccga gagaacgaaa aacggaatag cgatcagcga aaaactattg
    53701 atacttccca ccattttttg agctgaacta aaagcagaaa catcaaacgg tataactaac
    53761 aataaagtca tcatcgatga gaatgcaata cttatagcaa tcggcatccc taatacaagc
    53821 aacacaacaa atacaaaaaa taacactaat ccggcttcta ttgccatttt attcctccat
    53881 ttttatattt tttcttgaat tgctggcttg ttgcttaatg tatcgattag attgatcagg
    53941 ctatagataa tgatgagtac ccccgataca ggtactgaca gataaacata tcccatagga
    54001 atacttaaag aaggcgagat ctgccccatt gtcaatgtga cagcattcca tccgccatac
    54061 accatgatca tcgcagcaaa taccagaaat aaactttgaa caacaacttc taaaatttta
    54121 ctttttttgc cagttaattt ttcacttaaa accgttacgg ctaaatgtgc tttttgtccc
    54181 acaacatacg cggaagacaa catggcaaac cagacaagac cgaatcgaac aagctcttcc
    54241 gtgaccgtat ttggatttcc taaaaccgtt ctggaaaata cttgccataa aacaacagcg
    54301 accatcccaa caagcaaaac agctcccagg atctctaata ccttgtttaa aatcattttc
    54361 atcacagcta cgctccttgc tcttggatct tttgataaag gtctttgtag gtaggatcgt
    54421 ttttgaatat atcatgaagt ggggcaactg cctctcgaaa cggtgtatta tctacatcat
    54481 tgaattggac tccatgttct tcttgtgcta aagctttttc ttcttcaatt gcttttgcaa
    54541 aagcttcaat ttcaaaaacc gttgattctt tcgctgcttc ttcaatggct ttttcctgct
    54601 catcagttag ctgttctttg atggcatcat tcatgatgat gatatctggc acacgtgtat
    54661 gctcatcata ggaatagtat ttagccacac caccatgtcc agctgtatac aagacaaatt
    54721 cattgttttc tgatccgttg atcaaattgg attgaagaga cgtgtacact tcatcacttc
    54781 ccataggtac gggcgaaccg cctaaaagct ctaccatttt gatagcggtt tcgctttgca
    54841 tcacacggat cttctttccc ttcaaatctt ccggcgtatg gatcggaccg tctaccatat
    54901 aaaagctgcg ctgaccagaa tcataatagg tcaatccggt aaatcctaaa tttttggtcg
    54961 attggtagat tggctccatt atttcttcat tatccatcac cttaaagaaa tgttcttcat
    55021 cattgaatag ataaggaact gaaaaaatcg aataatcttt agagaaactt tccaacgctg
    55081 aggcacttac ttttgtgaaa tctactgctc ctgactgtgt caattcgatc agttcacgtt
    55141 ctccacccag ttgtccatct gggaaatact ggatctgtac ggaaccatct gttttttctt
    55201 ttactaaacg cccaaattca ttcattgcat caattactgg ctggctgttg cttgcataag
    55261 caaagcgcaa ggtaactatc tcatttacat ctgcatctgc gacagttgcc acactagaat
    55321 tatttccgca gccgcttaga cctagcagac ctaggctaag cgaacttgct gcaaacagca
    55381 gtgatgtttt tttcatatgg gtttcctttc ttttaacaat aagagacgag caattctgtc
    55441 aaacaaagca ccgttaacga ctgtccataa ggcattgctg tgattccgat attccgataa
    55501 aaatctaaac tgtcccccat tcctgtcccg attgaaacgt tttgaacttc tcctttttca
    55561 tcgatttgtt caagcaatcc ttggatagca cgataagcga cttctttata ttcttgtggt
    55621 aaataacgtt tatggaccgc tttcaaaata ccgtaagcaa atcctgcagt tgcagaagat
    55681 tctacgtaag aagaaggatc atccaacaac gtatgccata aaccgctttc agactgataa
    55741 gtttttaatg cacgcacttg tgcttcaagt gtttcgatca ataatttgcg caatgcatct
    55801 tctttcgata attctaagat ttcaatgatc tctggaatag caatcgtcaa ccagcagttt
    55861 ccacgagccc aaaatgcttc ggcatagtta tgatttcctt caaacgtcca gccgtgatac
    55921 cacaatcctg ttcgtttatc catcaagtat ttgatatgga tcataaactg gtaacgagct
    55981 tcttctaaat attctgggcg attcaacagt ttcccgattt ttgccaaagg aagcactgtc
    56041 atcatcaatg tatcgtccca caactgattc ttattttccg gtccatatgt ggcatgctgt
    56101 aacccgtctt catttgtccg aggcatgtca ttcatgaccc attctgccca ttgttctaaa
    56161 taaggcaaat agcgtgaatc tttcgtgtct tcatacaagt aagccatagt caatagcggt
    56221 gccattgtat tgacgttttt gggcggagcg ccttcttcca tacgatcctc aaaccattca
    56281 ttgaccactt gataagcgcg aggatttttt gtcatctggt aatttttata aataccatat
    56341 agaccgacac cttgtggcca attccacact gtccagcttt tgtcatctac tttcagtcca
    56401 tcaaagtcca acaaaaattc accattatcg tctttgatcg ttgtcaggtt ttcaacgagc
    56461 aggtcaattt tttctttcac taattcttta ggtacaaaaa tctcttcttt aataatcatc
    56521 tttacgccac ctttttaaac gctttcattt tatatcctta atatagcagt aataccgtcc
    56581 actataaata tcgctttttg aacaaaaact gtctaaaatt gacctaaatg ttaaataaca
    56641 aaagtaactt tcagttactt ggaaattaaa aaaagaactt ggagaaacaa cgttttccaa
    56701 gttctttttc atctattttc ttatcttttt tctacgcttt cttttgctta tccgttgtaa
    56761 gcatcaatga tgatctgttt caattcagag atcaatggtt ctttcggatt agctgtcgta
    56821 cattggtctt cgtaagctaa ttcagccata cggtctacgg ttgtatctag tgtttcttgt
    56881 gtgactcctt gagcttttag actcatgttg atgccgacag attttcctaa ttcataaaca
    56941 gccgttgcta aagcttcgac taattcctct gttgtttcgc ctttcagacc taagaatttt
    57001 gcgatatctg cgtaatctgt atctgcacgg aaatagtcat atttagggaa catcgcatgt
    57061 ttttgtggat cttttgcatt atagcggata atatgcggta aaagaatcgc atttgtccgt
    57121 ccatgaggga tcccgtattc cccgccgatt ttatgtgcga ctgagtgaca aatacctagg
    57181 aatgcgttag caaacgccat accagccatt gtagaagcgt tgtgcatttt ttcacgtgat
    57241 tccatatcag gtgtttttac tgatttttct aaatagtcaa agactaactt gattgcttgt
    57301 aggctcaatc cacgtgtata atctgaagcc atgactgaaa cataggattc gattgcatgt
    57361 gttaacacat ccatcccggt atctgctgtg acagatgctg gaactgacat gacgaattga
    57421 ggatcgacga ttgcgacatc tggcgtcaat gcataatctg ctaacggata tttcacatgt
    57481 gtttcactgt cagtgatgac cgcaaatggc gttacttcag aaccagtccc tgatgttgtc
    57541 gggatacata cgaattgtgt tttttctggt ttgtcgatct tatatgtccg tttacggata
    57601 tccaagaatt tttgttttgc accaaagaaa gatgtgtctg ggtgttcata gaacatccac
    57661 attcccttag ctgcgtccat tgcagaaccg cctccaagag cgattactgt atctggcttg
    57721 aagtctgcca tcagtttcgt tcctgcgtat acggtatttg tcgatgggtt cggttcaaca
    57781 tctgagaaga cttcgatctt cacatcattt ttacgtcgtg ccaatacttc acgaactgta
    57841 tcgcagtaac cgaattggac cataccaggg tcacaaacga tcatgacacg ttcaacgttt
    57901 tccattttct ctaagtacaa tagagagttc ttttcaaaga agatttttgg cggtaatttg
    57961 aaccattgca tgttatttct ccgtttcgct acagttttga cattgattaa gtttacagca
    58021 gagacgttac gagaaacaga gttcttacca taagaaccgc atcccaaagt caatgacgga
    58081 atcatttcgt tatagatatc accgatgccg ccttctgcag atggtgtatt cactaaaata
    58141 cgacaagctt tcattcgaag accgaatttg acatgcaact cttcgttttc tgaatggata
    58201 actgctgtat gtcctaatcc tccaagattc aacatacctt cacacaaatc aaacgcatgg
    58261 tccgtgttgt tagctttgat catggcaaga actggagaaa gtttttctct tgatagcgga
    58321 taatctgggc ctacgccttc caattcagca atcagcattt tcgttccttt tggtacactg
    58381 attcctgcca attttgcgat ttccatcgca gaatgtccaa cgattgatgg attgaccgca
    58441 tattttcctt cgttcatcac tgcatcttct aatttttgca attcatctgg ttttacgata
    58501 taaacttggt gtgcttggaa ttctgctttt actgctgcat agatttcttt atccacgatc
    58561 acagcttgtt cagaagcaca aatcattcca ttatcaaatg ttttcgaaac gatcaaatcg
    58621 ttcactgcac gtttaatttt tgctgttttt tcaatataag ctggtacgtt acctggacct
    58681 acacctaaag ctggttttcc tgtagagtaa gcagacttga ccattccagc tccacctgtc
    58741 gctaagacga tcgcgatacc tggatggttc attagcatag aagtcgcttc gatcgaagga
    58801 tgttcgatcc attggataca attttcagga gctccggcag cgatagcagc atcacgtacc
    58861 actcgtgctg cttctgcaga agatttttgg gcacttggat ggaaagcaaa aacgattgga
    58921 tttcttgttt tcaacgcgat cattgatttg aaaatcgttg tggaagtcgg attcgttgtt
    58981 ggagttactc cgcaaaccac accgactggt tcagcaattt caatcaaccc tttttggaca
    59041 tcttcattga tcacaccaac tgttttatca tgtttgatat tatgccaaat atattcggac
    59101 gcatacatat ttttgattgc tttatcttcg taaacaccgc ggcctgtttc ctctacagcc
    59161 atcttcgcta atggcatatg ttgatccaaa gctgccattg ccatttcatg cacgatatgg
    59221 tctacttttt cttgatcaaa gctttccatt tctttcaatg ctgcatttgc tttttctgcc
    59281 aaatcatcaa tcattgcttg tacgtcaatt gtcttcgttt tttctttttc tattacttta
    59341 gccatcatta tcctcctaat gggtcatggt tatttgttaa ataattcaca tgtatatcat
    59401 acaatattta tttttct
//
LOCUS       DAZKKW010000009        59390 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.009, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000009 DAZKKW010000000
VERSION     DAZKKW010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 59390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59390
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.009"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(59..187)
                     /locus_tag="ACG8PO_000515"
     CDS             complement(59..187)
                     /locus_tag="ACG8PO_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFQ0205869.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            complement(190..1563)
                     /gene="rlmD"
                     /locus_tag="ACG8PO_000516"
     CDS             complement(190..1563)
                     /gene="rlmD"
                     /locus_tag="ACG8PO_000516"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFQ0205870.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            complement(1674..2729)
                     /locus_tag="ACG8PO_000517"
     CDS             complement(1674..2729)
                     /locus_tag="ACG8PO_000517"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFQ0205871.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            complement(2739..4169)
                     /gene="gatB"
                     /locus_tag="ACG8PO_000518"
     CDS             complement(2739..4169)
                     /gene="gatB"
                     /locus_tag="ACG8PO_000518"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HFQ0205872.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            complement(4166..5638)
                     /gene="gatA"
                     /locus_tag="ACG8PO_000519"
     CDS             complement(4166..5638)
                     /gene="gatA"
                     /locus_tag="ACG8PO_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HFQ0205873.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKATFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            complement(5638..5943)
                     /gene="gatC"
                     /locus_tag="ACG8PO_000520"
     CDS             complement(5638..5943)
                     /gene="gatC"
                     /locus_tag="ACG8PO_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA];
                     GO_process: GO:0006450 - regulation of translational
                     fidelity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HFQ0205874.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            complement(6049..8085)
                     /gene="ligA"
                     /locus_tag="ACG8PO_000521"
     CDS             complement(6049..8085)
                     /gene="ligA"
                     /locus_tag="ACG8PO_000521"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFQ0205875.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTVKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            complement(8102..10336)
                     /gene="pcrA"
                     /locus_tag="ACG8PO_000522"
     CDS             complement(8102..10336)
                     /gene="pcrA"
                     /locus_tag="ACG8PO_000522"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HFQ0205876.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLANPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            10491..12050
                     /locus_tag="ACG8PO_000523"
     CDS             10491..12050
                     /locus_tag="ACG8PO_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HFQ0205877.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQKVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            complement(12161..14092)
                     /locus_tag="ACG8PO_000524"
     CDS             complement(12161..14092)
                     /locus_tag="ACG8PO_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-specific PTS transporter subunit EIIC"
                     /protein_id="HFQ0205878.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAAAEA"
     gene            complement(14110..15024)
                     /gene="pfkB"
                     /locus_tag="ACG8PO_000525"
     CDS             complement(14110..15024)
                     /gene="pfkB"
                     /locus_tag="ACG8PO_000525"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFQ0205879.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(15021..15773)
                     /locus_tag="ACG8PO_000526"
     CDS             complement(15021..15773)
                     /locus_tag="ACG8PO_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFQ0205880.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLFNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(15932..16141)
                     /locus_tag="ACG8PO_000527"
     CDS             complement(15932..16141)
                     /locus_tag="ACG8PO_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0205881.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(16152..17480)
                     /locus_tag="ACG8PO_000528"
     CDS             complement(16152..17480)
                     /locus_tag="ACG8PO_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0205882.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            17616..17771
                     /locus_tag="ACG8PO_000529"
     CDS             17616..17771
                     /locus_tag="ACG8PO_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPJ_0845 family protein"
                     /protein_id="HFQ0205883.1"
                     /translation="MGLKFERTDNLDKLFESFAVDPEKTVAPKEEIDRFLKPQPQNEK
                     KKDKKQK"
     gene            complement(17845..18432)
                     /gene="yihA"
                     /locus_tag="ACG8PO_000530"
     CDS             complement(17845..18432)
                     /gene="yihA"
                     /locus_tag="ACG8PO_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFQ0205884.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(18515..19765)
                     /gene="clpX"
                     /locus_tag="ACG8PO_000531"
     CDS             complement(18515..19765)
                     /gene="clpX"
                     /locus_tag="ACG8PO_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HFQ0205885.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVERAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            complement(20012..21301)
                     /gene="tig"
                     /locus_tag="ACG8PO_000532"
     CDS             complement(20012..21301)
                     /gene="tig"
                     /locus_tag="ACG8PO_000532"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFQ0205886.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            complement(21455..22387)
                     /locus_tag="ACG8PO_000533"
     CDS             complement(21455..22387)
                     /locus_tag="ACG8PO_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0205887.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLNEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            complement(22544..23203)
                     /locus_tag="ACG8PO_000534"
     CDS             complement(22544..23203)
                     /locus_tag="ACG8PO_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HFQ0205888.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSIYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAIFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            complement(23206..24231)
                     /gene="sppA"
                     /locus_tag="ACG8PO_000535"
     CDS             complement(23206..24231)
                     /gene="sppA"
                     /locus_tag="ACG8PO_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFQ0205889.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELASNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAMR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            complement(24244..24534)
                     /locus_tag="ACG8PO_000536"
     CDS             complement(24244..24534)
                     /locus_tag="ACG8PO_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0205890.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAEAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            24944..26749
                     /gene="glmS"
                     /locus_tag="ACG8PO_000537"
     CDS             24944..26749
                     /gene="glmS"
                     /locus_tag="ACG8PO_000537"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFQ0205891.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDSSDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            complement(26821..28176)
                     /gene="glmM"
                     /locus_tag="ACG8PO_000538"
     CDS             complement(26821..28176)
                     /gene="glmM"
                     /locus_tag="ACG8PO_000538"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFQ0205892.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICARYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(28200..29369)
                     /locus_tag="ACG8PO_000539"
     CDS             complement(28200..29369)
                     /locus_tag="ACG8PO_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HFQ0205893.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            complement(29366..30253)
                     /gene="cdaA"
                     /locus_tag="ACG8PO_000540"
     CDS             complement(29366..30253)
                     /gene="cdaA"
                     /locus_tag="ACG8PO_000540"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HFQ0205894.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            complement(30442..34122)
                     /gene="nifJ"
                     /locus_tag="ACG8PO_000541"
     CDS             complement(30442..34122)
                     /gene="nifJ"
                     /locus_tag="ACG8PO_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HFQ0205895.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIVPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPAMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLENANPN
                     LAKALFEKTIQDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARAERRKKMEQATKEKKEK"
     gene            34320..34841
                     /locus_tag="ACG8PO_000542"
     CDS             34320..34841
                     /locus_tag="ACG8PO_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HFQ0205896.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLSCPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            complement(35540..36105)
                     /locus_tag="ACG8PO_000543"
                     /pseudo
     CDS             complement(35540..36105)
                     /locus_tag="ACG8PO_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(36284..36940)
                     /locus_tag="ACG8PO_000544"
     CDS             complement(36284..36940)
                     /locus_tag="ACG8PO_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0205897.1"
                     /translation="MRFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLRAGLITFIS
                     IVGFFLFTVIVFIWRTPAPPLPEITYGEFPFRLEYELNEELHVIEDTLIVEFDGFGMN
                     EGIGRYRRWTSRLASGEDLVLLLEVSDNKQIFYFPGPANYYMGDRLNGYNHTFPSASF
                     IERERGITRRDILHDKELLEQFGPLDQNTINEEELLNQYNIRLVNWEISEPIVNNFGD
                     "
     gene            complement(37508..38443)
                     /gene="whiA"
                     /locus_tag="ACG8PO_000545"
     CDS             complement(37508..38443)
                     /gene="whiA"
                     /locus_tag="ACG8PO_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HFQ0205898.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            complement(38477..39475)
                     /gene="yvcK"
                     /locus_tag="ACG8PO_000546"
     CDS             complement(38477..39475)
                     /gene="yvcK"
                     /locus_tag="ACG8PO_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HFQ0205899.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAIDR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            complement(39472..40356)
                     /gene="rapZ"
                     /locus_tag="ACG8PO_000547"
     CDS             complement(39472..40356)
                     /gene="rapZ"
                     /locus_tag="ACG8PO_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFQ0205900.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            complement(40491..41222)
                     /locus_tag="ACG8PO_000548"
     CDS             complement(40491..41222)
                     /locus_tag="ACG8PO_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HFQ0205901.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLQPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTVLQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSKALDTASEK"
     gene            complement(41226..42491)
                     /locus_tag="ACG8PO_000549"
     CDS             complement(41226..42491)
                     /locus_tag="ACG8PO_000549"
                     /EC_number="6.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HFQ0205902.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            complement(42754..45570)
                     /gene="uvrA"
                     /locus_tag="ACG8PO_000550"
     CDS             complement(42754..45570)
                     /gene="uvrA"
                     /locus_tag="ACG8PO_000550"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFQ0205903.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQDIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            complement(45580..47574)
                     /gene="uvrB"
                     /locus_tag="ACG8PO_000551"
     CDS             complement(45580..47574)
                     /gene="uvrB"
                     /locus_tag="ACG8PO_000551"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFQ0205904.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQVRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKNAAKALDFETAANVRDMILELKASK"
     gene            47833..49335
                     /locus_tag="ACG8PO_000552"
     CDS             47833..49335
                     /locus_tag="ACG8PO_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HFQ0205905.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            49337..50074
                     /locus_tag="ACG8PO_000553"
     CDS             49337..50074
                     /locus_tag="ACG8PO_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFQ0205906.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(50442..51608)
                     /gene="dnaJ"
                     /locus_tag="ACG8PO_000554"
     CDS             complement(50442..51608)
                     /gene="dnaJ"
                     /locus_tag="ACG8PO_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFQ0205907.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(51759..53588)
                     /gene="dnaK"
                     /locus_tag="ACG8PO_000555"
     CDS             complement(51759..53588)
                     /gene="dnaK"
                     /locus_tag="ACG8PO_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFQ0205908.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            complement(53639..54202)
                     /gene="grpE"
                     /locus_tag="ACG8PO_000556"
     CDS             complement(53639..54202)
                     /gene="grpE"
                     /locus_tag="ACG8PO_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFQ0205909.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            complement(54296..55339)
                     /gene="hrcA"
                     /locus_tag="ACG8PO_000557"
     CDS             complement(54296..55339)
                     /gene="hrcA"
                     /locus_tag="ACG8PO_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HFQ0205910.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEEKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            complement(55574..56740)
                     /gene="hemW"
                     /locus_tag="ACG8PO_000558"
     CDS             complement(55574..56740)
                     /gene="hemW"
                     /locus_tag="ACG8PO_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HFQ0205911.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMRLMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYRDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            complement(56756..56983)
                     /locus_tag="ACG8PO_000559"
     CDS             complement(56756..56983)
                     /locus_tag="ACG8PO_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HFQ0205912.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            complement(57303..57626)
                     /gene="relB"
                     /locus_tag="ACG8PO_000560"
     CDS             complement(57303..57626)
                     /gene="relB"
                     /locus_tag="ACG8PO_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB family
                     antitoxin"
                     /protein_id="HFQ0205913.1"
                     /translation="MRFFKRGNYRAMITVRVSDAKKEWLSYMAEFYGITLSELLKNYS
                     MEQLEDEYNRQTAEIAHKHWIRSGKTTVFMEESLMNLVGWNDLPFKIHARNSEAALIG
                     KYVYW"
     gene            complement(57969..58415)
                     /locus_tag="ACG8PO_000561"
     CDS             complement(57969..58415)
                     /locus_tag="ACG8PO_000561"
                     /inference="COORDINATES: protein motif:HMM:NF013964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFQ0205914.1"
                     /translation="MKIKIYNQEENQTRHIRNLSNAKLEEEARKVSNYNKKMAAKYVN
                     EEEFCFSKTKIYKRNPFVSEYVKRLAKGICQLCQEKGPFIKDGVPYLHCHHIEYLSQG
                     GKDVIENCIALCPNCHARIHELELPDDKEKLLKIVEKRENSFSKIP"
     gene            complement(58396..58992)
                     /locus_tag="ACG8PO_000562"
     CDS             complement(58396..58992)
                     /locus_tag="ACG8PO_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HFQ0205915.1"
                     /translation="MNPTLITKKELLKKLDISTGVLANLIRNGMPKEGEMFNLDKIIT
                     WRENWSKNILGELEVGRVYTNKEISEKFKCSKQGGMRRSHQTNTLVLFSDQTGSNVYK
                     DKWLNGILQYTGMGLKGDQVLDKNQNKVLANSKSNFVKIHLFETFKPKEHTYLGEVYL
                     AGQIYTVNEKDSSGNSRKVYKFPLALINQEQLIEDKDI"
BASE COUNT        16402 a        12849 c        10314 g        19825 t
ORIGIN      
        1 taaaaagact aagaaacaat aacaaaagtt aacacttctt agtctttctt tagcacgtct
       61 atttattaaa gattttaggg ccttttctca tgtttttacg atcattctta ggtgtttcca
      121 tttgtttgga tcggcctcca ccttgtttct ttttgatcaa ttctttcatt ttttcttttt
      181 ggtccatttt tattctcctt taacgaatga agcgactaat tcgatatgat gggtttgcgg
      241 gaaaagatct acaggctgaa ctttcttcac ggcataacct gcttctgcaa ataacttagc
      301 atctcttgca aaagttgccg gattacatga gatatagacg attcgttctg gtgacatttc
      361 gattgctgat tcaataaaat gatgatctaa tccttttcgt ggagggtcaa ctacgatcac
      421 agatggtttg atcccttttt cttgccattt tttcataact ttttctgctt ttccgacttc
      481 ataatgaaca ttttcgattt tattatttaa agcattgaac tgtgcatctt caattgcttc
      541 tggaataact tccatcccat acaccttatc cactctgtct gctaaagaca agccaatcgt
      601 acctattcct gaataagcgt cgatcacaat gtctttttta tctaactcgg caaactcgat
      661 ggctgtttgg tacagatact ctgcttgttg agtatttact tgataaaatg actttgctga
      721 gattcgatag gtttttccta atagctgatc ttcgatatag tctctgccat ataaaacttt
      781 ttctttgtct cctaaaatca catttgtttt ttcttcattg atattttgga tgaccgaaac
      841 cacttctggc aattcttcat gaatagcttc agcaatttcc ttggatttaa agaatttttc
      901 tttacgagta actaaaacga ccatcatttc atgggaataa tgaccacgac ggataatgat
      961 gtgacgaagc tgtccttcat ttttttcttc attatatcct cgtacttgga accgttgcaa
     1021 gatatctctg actactttga ttgcttgatc gatgacagga tcttggatat aaaaatcttc
     1081 gatagggact agatcatggc tattcttttt gtaaaaaccg gtggctaact gtccatcgat
     1141 acggcgaaca gggatctgtg ctttattacg gtagccgaat ggttctgcca ttccgaccgt
     1201 tggaagtaca ggaacttctg gcatcttagc aatcttcgcc attacatgct ctacttgttt
     1261 ttgtttgaag acaagctgtt gttcatactt caaatgggct aaaggtgcga tacctatgcg
     1321 aagtaatgca ttgttttcta caggctgacg atcaggtgat tgtgtaataa atgtttcgac
     1381 ttttccatat ccaaacttat tgcctgcttt tactactagt atttctactt cttctccagg
     1441 gagtgcattt tcgataaata atggataacc atcgattttt gctaccccca ttccttcata
     1501 actcaagtcg acgatttcga ctgtatatcg ttcgtttttc tttactattt gctgttctgt
     1561 catattttgc tcctttaagc ctatttttca atcctactga actctactat aaatcagcta
     1621 attacacaac taaaaccggc actgacctta ctacgttcag caccggcttt tgattaattc
     1681 ttttgattag gtgtttcgtc tgctattttt ccgtcaccgt cgatatcttc atccgttaaa
     1741 cgttcgactt cttttacgaa ttctttgctg acaacctcta attcttcttc atctgtacct
     1801 gtgattgctt catcaggaat ctgatcggta ttggcaaaga actcaatgtg ttgatgaaga
     1861 ttttggaaat gcatcggtgc ttccccgccg tattcaccat ctaagttgat cattactggt
     1921 cgattgatat catcttctgt ttcaacgaac aaactgcttg ttttggtata gattattctt
     1981 ggatcatcga catgtcgacc gccattcagc atgagtgctg caatatgcaa gatctcaaag
     2041 atatttgctg tcttcacaat gattaaagaa aacttcccat catccaattt ggcatctggc
     2101 gcgatctgtt cgaatcctcc aacagagttc gtcagcccta ggaaaaacat cgaagcattt
     2161 ccgatatatt ccccttcatc atacttcaag cgcattttga ttggctttac tcgagggagc
     2221 atctctgctc ctttcgcaag ataagcaaga taaccaaaaa tactctttag ttctgatgga
     2281 acttcataag tcaattctgt caaatagcca cctgcagcga tattgatgaa gtaactgtta
     2341 tcagaaaggc cgatgtccat ttttacggtt tggtgttttt ttatcacttc tgctgctgct
     2401 ttaatattat ctctaggaat ttttaatgct cgagcatagt cgttcgttgt accagctggg
     2461 atgatggcca ttttaggacg gtgttcaagc ggagcgattc cattaaccac ttcgttgatt
     2521 gtgccatcgc caccagctgc cactactaga tcaaaaccta gttctgcgac tctacatgct
     2581 tcattcttgg ctgaatctgg ttctggtgta gttgcatagg cgctggcttc aaatcctgct
     2641 tcttctaatg cctgtaggat atccgccaaa ttttttttca ataattcttt tcccgaggtg
     2701 gggttataaa ttacgcgtgc ttttttcatt ttcagcctct atcgtttcgc taattcatct
     2761 ttaagtagct tattcacgac accaggattt gcttgtcctt tagttgcttt catgatttga
     2821 ccgactagga agccaaccgc acgatctttt ccatttttga aatcatcgac agattgttgg
     2881 ttgttatcca aaatttcatt aatgattggc agtagtttgt tcggatcaga taattgcacc
     2941 caaccatttt tttcaactac ttcttgggct tctccgccat ttgtgatcaa ttcacggaag
     3001 acttttttgg cgatttttga gctgatcgtt ccatcggcga tcaaacgaat cattcctgct
     3061 aagttctcag gtgtcaattt tgtctcagct aattccagtt tctcactgtt caagtaagca
     3121 gaaacttcac ccatcaacca gttagatgct tgcttggcat ctgcaccatt ttccaaaaca
     3181 gcttcaaaaa aatcagacat ttcttttgta agtgtcaata ccattgcgtc atactctgga
     3241 agtccaaact cacggatata gcgttcgcga cgagaagctg gcatctcagg caaactagct
     3301 tttacttttt gaatccattc gtcatcgatc acgaatttcg ggatatcagg ctctgggaag
     3361 taacgataat cactagaccc ttctttgaca cgcatcaaaa ctgttttatt cgttgtttcg
     3421 tcaaaacggc gtgtttcttg ttggatttct cctcctgaaa gaagaacttt tgcttgacgt
     3481 ttttcttcaa aagctaaacc ttttttcacg aaagataaag agttcaagtt tttcaattct
     3541 gctttcgtac cgaactcttc ttgtccataa gggcgaagag aaatattggc atcacagcgc
     3601 atagagcctt cttccatttt cacatcgctg acttctgtaa attggataat tgatcgcaat
     3661 gcttctagat aagcgtatgc ttcttctggt gaacgcatat cagcttcaga aacgatttcg
     3721 atcaaaggcg tgccttgtcg gttcaaatca acgtaagaat aaccgccaat accatgcatg
     3781 ttttttccag catcttcttc taaatggaca cgttcgatcc ggattttttt ctttttacct
     3841 tctacttcga tttcgatcca tccgtcatga ccgattggtt gatcaaattg ggaaatttga
     3901 tatgcttttg gattatctgg gtaaaagtaa tttttccggt caaaatgagt atctttagag
     3961 atttcacagt ttaatgccaa agctgctttc atcccgaatt ccagcgcagc tttattcatt
     4021 actggcagca cgcctggata accccagtcg atcacgtttg tattgctgtt tggttctgca
     4081 ccaaaatgag ctggcgctgg agagaaaatt tttgagttgg tttttaactc gacgtggact
     4141 tctagtccaa tgactgtttc aaaattcatt cgtctttccc tcctaaaata gctggttgtt
     4201 gtttatggaa ttctgttgct tgttcaaacg catatgccgc ttgatacatc gttttttcat
     4261 caaaataatt accgatgatc tgcaatccaa ctggtaatcc ttctgataaa cctgccggta
     4321 cagacatacc tggaagtcca gccaagttga cagggatcgt taagatgtca ctcatgtaca
     4381 ttgtgattgg atcgttaatg ttctcaccga ttccgaatgc aactgttggt gaagatggcc
     4441 cgatgatcaa gtcataatct gcaaagactt tttcaaaatc tcgtttgatc aatgtacgga
     4501 cttgtcccgc ttttttgaag taagcgtcat aataaccagc acttaaggag aatgtaccta
     4561 acatgatacg gcgttttact tcttcaccga atccttctga acgtgagttg acatatacat
     4621 catctaatga tttcacattt tctgagcgat aaccgtagcg gataccatcg aatcgttgca
     4681 agttggaact tgcttctgat gaggcaatga tataataaac agccacaccg tattttgaat
     4741 gaggcaagct gacttcttcc acaacagcac ctaatgaacg gaatgtttct gctgctttca
     4801 atacagcttc ttttacgcca ggggccacac cttcgcctaa gtattcttta ggtaaagcaa
     4861 tcttcattcc tttgatgtcg cctgtcagtc cctctgcaaa atcaggaact gaaacgcctg
     4921 aagaagtacc gtctttttcg tcataaccgc tgatagctgt caatgctaat gcattatctt
     4981 tcaccgtacg ggtcattggt ccgatttggt caagagatga agcaaaggca atcaaaccaa
     5041 aacgtgagac acggccataa gtaggtttca tccctacgat gccgttaaaa gctgctggtt
     5101 ggcggatact tcccccagta tcactaccta aagaaactgg aatttgtcca gctgcaactg
     5161 ctgctgcaga accaccagaa gagccaccag gtacttttgt tgggtcccaa gcattttttg
     5221 ttttcttgaa ataagaagtt tctgtacttc cacccatagc aaattcatcc atgtttagct
     5281 taccaacagg gatcatatct gcttgataga ttttatccat gaccgttgca tcataaattg
     5341 gttcaaaatt gtaaaggatc ttagatgcag cggttgtcaa gatatctttt gttacgatat
     5401 tatccttgat accaattgga ataccagcaa ggacgttgct ttcatcaatc cctttttcat
     5461 ctaaagcttt tgcctgttcc aatgcttttt cttcgttcaa tgtaatgaat gcttcgacct
     5521 caggctctgt ttctttgatt cgttcaaatg ttgcttttgt caaatcttgt acagtgattt
     5581 ctttcgatac aagcatatga tgcagttctt ctagagaacg attgtgtaat tctgtcatta
     5641 tgcgccagcc tccccattat cgatgattgc gggtactttg ataaagccgt cttctgtttc
     5701 cggcacattt ttcaataatt catcgcggtt ccatccagct tgcgcccgat cttgtcgcat
     5761 cacgtttact gtttcaacca cattcgacgt aaatggaaca ccttctgtat ctacttcttc
     5821 cagcatttca accatatcga tgattttacc taattggtcg gtaaaacttg caagttcttc
     5881 atctgcgaac gcaagttttg ctaatttggc aacgtgtttc acttgttctt cactaatcgc
     5941 catactaccc cttctttcta aacgagttaa aaactttgct ctctatttta acacaagagt
     6001 gccgtgtctg aaattgttta ttgaaatttg tctgaaaaaa ctcatttttc attcttctac
     6061 tgagcgactt ttagccaatg catctgccat ttgatcttct gtccaaactt cgattcctaa
     6121 ttcttgtgct ttggtcagct tgctgccagc atcttctcct gctaccacga tatccgtttt
     6181 tttcgatacg ctaccagtca ctttcccgcc aagattttcg atcgtctctt ttgcttcttc
     6241 acgagtaaat cgtgttagtt taccagtaag gactactgtt ttttctttaa aaggagactc
     6301 gacttcttgc aattgggcat ttcgaagccc tttgtattcg aaattgacgc ctgcttgttt
     6361 taattcattc atcaattcat ggacttcttc gttactgaaa taagtgacta cactgtcggc
     6421 aatgatatca ccgatcgtat caagcgcaat aatcgactca tagtcacttt tactcaatgt
     6481 ttctagatca ccgaaatgtt ctgctaaaat ttttgctgct ttcgctccga catgtcggat
     6541 tcctaacccg aaaatcaacc gctcgaccga attatctttg ctgttgtcga ttgctgtcaa
     6601 aatattattt gctgattttt cttttatttt gtccaacgtc aataactctt cttctgtcaa
     6661 tttgtaaaga tcagcaacat ctgctactag ttttttatca tacatctgtt ctagtacgcg
     6721 cggccccaac ccatcgatat tcatcgcatt tcttgaaaca aagtggttca aaccttcttt
     6781 catttgagca gggcatttag gattgatgca ccgtaaggct acttcttcat ccaaatggac
     6841 aagttcactt ccacaaactg gacaatgtgt aggcaattgg tattcttcgc tgtctttagg
     6901 tcgtttatct aacacgactt gcgaaacttc tgggatgatg tctcccgctt tataaatcag
     6961 aactgtatct ttcaagcgga tatccttcaa cttaatataa tcaccgttat gcaggctggc
     7021 acggcttact gtcgttcctg ctactcggac tggtgtcatg atagctgttg gcgttactac
     7081 ccctgttcga ccgactgtcc attcgatgtt ttcgattagc gtttcaactt cttctggcgg
     7141 aaacttgtaa gctgctgccc atctcggtgc tttgacagta aaaccaagtt gatcttgtag
     7201 cgagaattca ttcactttga tgacgattcc atcgatttca tagggcaaat ccacacgttt
     7261 gtcatgatat tcttcaatgt atgaccacac ttcatcaatc gaatgacata gacgtttttc
     7321 ccgattcgtg tggaatccaa ttttttctag ctcttctagc gcatcatact gggtttttgc
     7381 ttgcatagga ccgaaatcag ctactgtgta taaaaaagtg tccagatttc ttttcgctgt
     7441 gattttagaa tctaactgac gcaaagaacc agctgcggcg ttccgaggat tcgcaaagat
     7501 atcttttccc tctgcttctc tgtcttggtt caattggaca aatgaccgtt ttggcatgaa
     7561 acattcgcct cttacttcga tgttcattgg ttcttttaat ttgattggga ccgaccggac
     7621 agtttttagg ttttcagtga tgttttctcc tacagttcca tccccgcgag tcgcacctct
     7681 cacgaaattt ccatcttcat aacgcaaaga aacggaaagt ccatctattt ttagttcaca
     7741 gcaatagtca actacacgtc ccactgcttt ttgtacacgt tgatcaaaag ctatcagctc
     7801 ttctttgctg aaaacgtcgt tcaaactgta aagcggaata tcatgagtga ctttttcaaa
     7861 accttctaat acttttccgc ctactcgttg agtgggagaa tcagacgtga tcaaatcagg
     7921 atattgttcc tcaattgcta ctaattctgc gtattcttta tcataaacat agtcttccac
     7981 tgttggttta tcttctacgt agtattcgcg agaccattgg ttcagtcttg tacgaagttc
     8041 ttctgctcgt ttagcagctt cgtcaatcgt catttcatgt tccatcattt accctccact
     8101 actaagcttt ttcaattggc gcaaaggcag ccaaaagacg ttttactcct tgacttggga
     8161 aagccacatc tagttctaga tccttcgacg tcccgcttac tcggacgact gttcctagac
     8221 cccatttttt gtgtttgact ttatctccag gtttccagct gttattttcc cctccagtag
     8281 cacttttaga gggaacaacc gctgtctctg gctgtttata gctatatgct gtttttttag
     8341 cgaatacagg tgttttttta ggcgtaggac gcatgccttc gatttccagt aactcctgat
     8401 cgatttcttc tacaaaacga gaaggtcggt tatattgcgt ccgtccatat aatgttctgg
     8461 agaaagcatt tgttaaatac aacgcttctt ctgctcgcgt gatccccaca tatgcgagac
     8521 ggcgttcttc ttccagttcg ctttcttcca tcaatgctcg agataaagga aaaaccccct
     8581 cttctagacc aatcaggaat acaactggga attctaatcc tttggctgca tgcaaagtca
     8641 tcaatgtcac ttgggaagca tcttcttcta aattgtcaat atcggaaaca agtgccagat
     8701 catttaagaa gaccgttagt ttttcttctg gtgcatcagc gtcttcttca ttttgctgtt
     8761 cgaattgttt gtcgaattct tgtgtgaccg ttaaaaattc ctccaagttt tcgatgcgtg
     8821 cttgtgcttc taatgtattt tggattttta agtcttctag atatccgcta cgatccaaaa
     8881 cctctttcgt caattctgtc actgtcaagt atgggatcat ctgagtgact tcttggatca
     8941 tctctccaaa tgcacctaat tgctgtcctg cttttccacc gatatttgcc agatccacat
     9001 tttgcgctgc ttcaagcagt ggccattcat gaagagaagc aaaactgcgt agtttttcga
     9061 tagatcctgg tccgatgcct cgtttgggcg aattaacgat acgctcaaaa ctgattgaat
     9121 cttgaggatt tgccagcaca ttcaaatacg ctaaaatatc tttgatttct ttacgatcgt
     9181 agaacttgtg ccctccaacc attttataag gaatatttgc tttcaacaga gtttcttcca
     9241 ttacacgaga ttgtgcgttc gtccggtaca agatggcaaa gtcgccgtaa ttccgatgat
     9301 tgcttcgaat ctcttcttgc atccgatcta cgataaaacg ggtttcgtct cgttcattat
     9361 ctgcacggta gtaagtgatc ttgtttcctt ctttgttttc tgtccacaag ttttttggtt
     9421 ttcgattgct attgttttca atgacttggt ttgctgcgct aaggatgttt ttcgtggagc
     9481 gatagttttg ttctagcaaa attactgcag catctggata atctttttca aaatccaaga
     9541 tgttctgcat atctgcaccg cgccagccgt aaatactttg atctgcatct cccacgacgc
     9601 acaggttcct aaatctacct gccaataaat tgactagcgt atactgtgcg tgatttgtat
     9661 cttggtactc atctacatga atataatgga atttgttttg ataatatgtg agtgaatcag
     9721 gatgttcttc aaataatcga atcgtattca tgatcaggtc gtcaaagtcc atacattgat
     9781 tatattgaag ctctttttgg taagcagcat aacattttgc cgcaatctct tcgtaataac
     9841 tgccttgcat ttttgcaaaa tcctgagggg tctgcaggct gttttttgct tgagagattg
     9901 ttcctaatat acttcgagga tcatattttt tcggatcaat gttcagttct tttagtatcc
     9961 gtttcatcaa ggtcagctgt tcagaagaat cgatgattgt aaagttccgg ttatacccga
    10021 taaaatcaac atctcttctt aaaatacgca cacacatcga gtgaaaagtc gatacccaga
    10081 catcttcgcc tccagatgcg agaatcgcat tgacacgttc tttcatttct tttgcagcct
    10141 tattggtaaa agtaatcgct aggatgttcc aaggattgac ttctttttcc tcaatcaaat
    10201 aggcaatgcg gtgtgtcagc actctcgtct ttccacttcc tgcacctgcc attagcagta
    10261 atggtccgtc agtgtgaaga acagcttctt tctgacgggg gttcatcccg tttaataatt
    10321 cagctttgga tgacattaac aacatccttt cttttatcaa tcttgttaat tatagcattt
    10381 tcttggcttt cattaaacta gctagttcaa aatcatttgt ttctttttat ggatttttcc
    10441 ttgtcaattc gttgatttcg cgctaaatta aaagaaagcg aggcgtttga atggaaaaaa
    10501 aaggaaaaat caagaaatta gataagactc agatatattt tattctaaaa ggaatatttg
    10561 ttggactgct gacaggaatc gtcgttagcc tgtttcgatt atctatcgaa aagctaagcg
    10621 atttagttcg aagtatctat gaaatgagtc gggaacagcc gatttactta attggcctag
    10681 ctatttgctg catccttgca gcatttttcg tgggttatct ggtcaaaaat gaaccggata
    10741 tcaagggaag cgggattcca caagtagagg gacagcttcg cggggaatta tcaatgaact
    10801 ggttctctgt tttatggaaa aagttcattg gcggtgtact ttcagttggt gcgggccttt
    10861 ttctaggtag agaaggacct tctatacagc ttggcgcatc cattggacaa ggagctggac
    10921 aattgtttcg gagtccatct tcagaagaaa agattttaat ttctagcggt gcaagtagcg
    10981 gacttgcagc agcattcaat gctcctatcg caggattact ctttgtttta gaagaaatcc
    11041 atcactcctt ttctccactt gtttggctga catcatttgc ttcagcaatc actgctaact
    11101 tcgtctctct ttactttttc ggcttgagac ctgttttata tctaggtgac cttccttctt
    11161 tgccgttgaa atattatggt tccttagtga tcttaggagt acttctaggc atcttaggtt
    11221 ttatttatca gaaggtcttg ctggctttgc caaaatttta caaccgattg cctttaccag
    11281 cttattttca cgggttcgtt cctttcttgc tgattttgcc aattggttat ttatggccta
    11341 gtactctagg cggaggcaat caaatcgttt tagcttttgg acatgcttcc ttgccaatca
    11401 ttatgattat cggtttgttt gttcttcgat tcgttttttc catgatttct tacggtggtg
    11461 gtcttccagg ggggattttc cttccgatat tgacattggg ggctttaatc ggtagctttt
    11521 atggaaacat tatggttgat ttcgggatgg atccaatcta catcaaggat tttgtcgtta
    11581 ttgccatggc tggttatttc acagcgattg gaaaagcacc gctgacagca attattttag
    11641 tgacagaaat ggttggctct cttaatcatc tgatgccttt aggattggct gctcttgttg
    11701 cttatatcgt caatgacctt ctcggcggta atccaattta cgaatcatta ttggaacgtc
    11761 tacttggaaa tactcagacc gcctcactaa aaggtgcaag gacaactttt gattttccag
    11821 taacggcaga gagttcttta gacggattaa tggtacgaga cttcaattgg cctaaaaata
    11881 tgctgctaat ctctatacgc agaggaaatc aggaaatctt gactcacggt gatacgatca
    11941 tgaatgtagg agatattttg gttatcttga ctgatgaagc aaacttgcca agaatcaagc
    12001 aggaaatcga ggctaaatct tctatcacaa atttgaagca atcattttaa cgaacatgga
    12061 aaaactattt tacaacaact attcatctac actacttgat tctatatgat tctataaaaa
    12121 aagaagagac tgggaaaaac cagcctcttc ttttttattt ctatgcttct gctgctgcag
    12181 gagcttttct caataaacca taaatcacac cagatacgac tgctccgata gcaataaaca
    12241 ccaaatacat gaccgggtga ctcagtaaga atacaacgaa aatcccgcca tgaggagcca
    12301 gtaatttgat cccgaaagct ccgactaatc cgccagttaa agcagaacca ataataaaac
    12361 ttggaatggc acgaatcggg tcagctgctg caaatggaat cgatccttcc gtgacaaacg
    12421 atagtcccat cacgatgttc gtcaaaccag catcttgatc tgttttgctg aatttattct
    12481 taaacaaacg tgtagcaacg aaaattgcca atggaggtac cattcctcca gccataacag
    12541 aagccatcac gacacttccg ccagttgcaa cagtcgtagc taaagttgct gtaccaaaca
    12601 cataagcggc tttgttgatt ggaccgccta aatctgccgc catcattcca gctaataatg
    12661 caccaagtaa agcagcattc gaaccactca tacttgtcaa gaaaccgttc aatgcatcat
    12721 tgattgcttt catagggaca ttgaccagca acatcaagaa accagtaatc agtaagccaa
    12781 acactggata aaagaggatc gttttaattc catccaatga ttttggtaag ttcttgaata
    12841 atttacgtaa aaatacgatg acataaccag ctaagaatcc accaatcagt gcacctaaga
    12901 atcctgcacc gcctgtatta gctaatgcac cagcagcaaa tccaacgatc aaaccagggc
    12961 ggtcaccgat acttgaagca ataaatcctg ctaataccgg cagcatgaaa ccaaatgcag
    13021 cttgtccgat ttggttgaac cagctagcag cttgattgta attccctaga tttgataatt
    13081 gatcatgtgg cacgccaata aattggtcga tcatgaaaga aagcgcaata gcgattcctc
    13141 caccaataac gaaaggtaac atgtgtgata cgccgttcat caaatctttg taaattcttt
    13201 gtccgatagt accatccgca tttccttctt ctgaactgct ttcttttcca tcaccatgga
    13261 atactggtgc agtaccacta agggctagat tgatcagctc ttctggttta cggataccat
    13321 cactgacagg acgattgatc agttcttttc cgtcaaagcg attcatctct acttttttgt
    13381 ctgctgcaac gattacacct tctgcacggg cgatgtcttc agcagtcaat cggtttttga
    13441 ttccttctga accgttcgtt tcgactttga tctccacacc catttctttc gcttttttct
    13501 tcagagcatc ttctgccatg taagtgtgag cgatccctgt tggacaagct gtaacggcaa
    13561 caacgaattt tctttctgga tgagcagaag cttctgcagc ttgtgtttct tgttctgctt
    13621 tttcttctgc ttctttttgc tcttcagctg tttggaacaa gtcttggact tcttctggtg
    13681 tatttgcttc ttttaatttt cctacgaaat ctgcatcaat cagcaatcga gatagtgctg
    13741 ctaatgcttg caaatgtgtg tcgtttgcgc cttctggagc tgcgatcatg aaaaacagat
    13801 aagttggctg tccgtctaag gcttcataat ctactccttt ttggcttttt gcgaataaaa
    13861 ctgttgcttc tttaactgca ctgtttttcg cgtgaggcat ggcgattcca tcaccaagac
    13921 ctgtagaagt ttgtgcttcg cgggctagaa ttccttcttt atatgtttcg atatcggaaa
    13981 tccggccggc atcaaacatt ttttggacca tttcatcgat tgcgcctttt ttatcagtcg
    14041 cttgcaaatc catgatcatc gcttctttga ccattaaatc cttgatattc atgattatcc
    14101 tccttgaaac taatcaattt ttgtgatttc aacttctggc agcaactcat caatcaattc
    14161 gcgagctgcc aagtcatcag aaaaagctgt tgcgcttccg caagcaactc cccatttaaa
    14221 ggcttcgatt ggatctttcg tacggctgta agccccaata aagccggcaa tcattgaatc
    14281 ccctgcacct actgaatttt tcaatgggcg aactaataca ttcgttttat aagttccttg
    14341 ttccgtgaag agcaaagcac cgtctccagc cattgagatc agcacattct gtgccccttc
    14401 agctagcaat tttttaccat acggaatgat ttcttctaca gattggaagg ttgtattgta
    14461 aagctcagct aattcgtgat tgtttggttt gatcaacaat ggtttcttaa caagtgcatt
    14521 ttttaggtca tcacctgttg tatcaatcac gaaatctgca cctttttctc gaatgatatc
    14581 aatcagttct tgataaaatc cttttcgcag gctagccggc gcgcttcctg acaatacgac
    14641 gatatctcct tgtcctactg ttcctaatgc ttgcttcaac tcatcgattt cttcatctga
    14701 aatagatggg cccaaaccgt taatttcagt ttcagtatct gattttagtt tgatattgat
    14761 tcttgtatct tcttttacgg ttgtgaacaa agactggatt tgttcttttt caagccactt
    14821 cgaaataaat tcgccagtga accctcctaa aaaccctagt gctgtagatt gagaatcgat
    14881 ccgtttcaaa atacgggaaa cattgatccc ttttcctcca ggtaacttga agtcttcctt
    14941 cattcggttc aaatcgccta gttttaaccc atctacgcgt acgatataat caattgatgg
    15001 attaagtgtt actgtgtaaa tcattgtatt gcctccttaa ttgttgtctt ttcacggaat
    15061 tcattaacga attctaatgg acaacgctcg gttatgatcg tggctgcttc caaaggtgca
    15121 acagaggtga aggacaactg gttgaatttg gtatgatcaa tcaatacaaa tgtctcttcg
    15181 gaatttttca tggctaaacg tttaacagct gcctcttctg ggtccggagt cgtgaatcct
    15241 tgttcaagat gggcaccgtt cattcccata aatgctttat taaaatgaaa attttttagt
    15301 tgttctatcg cattagatcc taaaacagca ttagttgcca gctttatcgt tccacctaaa
    15361 ataatggtag caagctccaa atcaactaaa tttgccgcat gttgaacgga atttgtcact
    15421 actgtgatct gttttccttg cagaaaagga atcatttcta acgtagtaga gccagcgtct
    15481 aaataaatga catcttcgtc attaatggtt tcagctgccg atttggcgat ttgctgtttt
    15541 tcgtgaacgt ttttgagtga tttttcactc atattctgct caaaattcaa taattgctct
    15601 ttcttagctc cgccatgaac tctttctaga aacccactat tctccagctc ttgtaaatca
    15661 cgtcttatcg ttgattcaga agcattaaaa aggtttacaa gatcttgtga tttcacgatt
    15721 ttctgctgtt caagcaagtc taggattttt tgtctacgtt cctctgtaag cattttcacg
    15781 atcacctcaa tacaaataat agcatatttt ttttctactc gcaatataaa tcagtcaaaa
    15841 acgttcataa aaattataaa tcagtcaaaa aaaaataaaa aaccagtcaa aaagactggt
    15901 tttttatttt tttgtctatc atcaagccgt tttatcgttt tttattttcg cttcttgttt
    15961 ttttcatata aacagaagca ggttttcttt ccttatgaag aagataataa taaagtccca
    16021 tcccgccaaa catgaccaaa aaaccgatga ctgatcgaat aggaaaatta ggaacaaaaa
    16081 agagacagac ggataataat gatgccggaa ccattattcg ataatagagt tgtttatcca
    16141 ttttcttcac ctcatatttt ttgtaaagta atcaattgtt ctaattctcg gtaatctaaa
    16201 aattgcacac gcagatgctg ctcacctttc ttgacatcga attggatact tcgccgcttt
    16261 ttctttattc gtttgaaggt aacatcagag agtctatgtt tgatttcctt taacggtaat
    16321 tctgacaggg attctgaagg aacatagatt cttaaggata aaagatactc ttgatatttt
    16381 tcatctgtta cctctacttc tagatattcc ctaatataat aacaataagc ttcgattcca
    16441 ttatccaaag caacagtcgg atcgataact ttatagcaca tcatgcgcga gcgattctcg
    16501 tcaacccaag gaaaatacgc ctgagcattt agtgttccgc gaacacgcct ttctttataa
    16561 tctccttgtt gaatatcata actttttgtt acaaaaaatg aagcattctt tttaggaaca
    16621 ggttgcattt gtgcattctg atactggaca gattggatcg tatggtcagt gtaaatgtat
    16681 tcaacgaatg gcggtttcaa taaaccgaca ccattgtccc aagcaagatg atcaaaccca
    16741 agctggtccc taaaatagga ttgaaacatt tgattgtttg gcttttgcca gttaaagggg
    16801 tctcgataaa gctggataca tatgacttcg ttttctaatt ccttcacttt ttctacttgt
    16861 tggatgtcga gaaaaatcgg tttagggatg atttgatgat aatagcgact ataatacatt
    16921 tcccaacacg aagtgaaaca taaaccttgg taaaaaagat cgtatcctgg aaattggttg
    16981 caatcgacta ctacttcccc gttttgttgt ttcattttag gtaaatgttc gatttcatcc
    17041 tttttctcaa acaatagccg ttgagagatc tgttttgtat tgtgatagat aaactcgcta
    17101 taagctcgca tataggcgaa caccccatga gcaatcattt ttttctgaat gaagtctcga
    17161 atcaaaaatt gcttttcttt gaaaacagag atagacaata cattttttat caataattga
    17221 ttatcttcca agcgaaaact acattcgttc tcgtcatttc ctacaactaa ccaactttcg
    17281 tctcttaccg atgctagcat tattttttct tgaagctttc gaagatcata cggatccgct
    17341 tgttgtgtga aagagcggat aaatgttggc tggatcaaca gacgtgtcaa aatagcccaa
    17401 tcgcttattt cctgtttttt ttcttcttca tttaagtctg tgagctggat agtcatcaat
    17461 aatttatctt cgccaatcat tttctcacct ctttaaaatc tttttataat ttaattataa
    17521 ccaacgataa agaaaaacta aaactttatg tcttagaaac caatttggac gattacatga
    17581 tagactaagt aaaaagaaac aaagaggtgt ttacaatggg gttgaaattt gaacgtacag
    17641 ataatttaga taaacttttc gaatcatttg ctgtcgatcc ggaaaaaaca gttgcaccaa
    17701 aagaagagat cgatcgattt ctaaaaccac agccacaaaa tgaaaagaaa aaagacaaga
    17761 aacaaaagta agttaagctc aaaaaaagac ttaccagcca aacttttatg aaagagtttg
    17821 actggtaagt cttttttatt ctttttatga ttcaattgct tgttcgattg ctgcccacgc
    17881 ttcttctttt ccctgttttg taacggaaga aaagagaata aacgtgtcat tgcgatcaaa
    17941 atccaacgct tttttgattt cgctctcatg cttgttccat ttaccacgag ggatcttatc
    18001 tgccttagtg gcaacaacaa ttacagggat atcataatac ttcaaaaatt gatacatctg
    18061 gatatcttct tgagaaggct tatgacgcaa atctactaaa gaaacgaccg cacgtagctg
    18121 gtctctagtc gttaaatatg tctcgatcat ctttccccat tttgctcgct cagttttgga
    18181 tacttttgca tacccatatc ccggaacatc gacaaaatgt aatgcgttct cgatcaaata
    18241 aaaattcaat gtctgggtct ttcctggctt accggaagta cgggctaaat tctttcgatc
    18301 aatcaatgtg ttgataaaag aagatttccc tacatttgat cgtcctgcca gagctatttc
    18361 tggcaattgt gtatctggat attgttttgg tgccactgca cttatgataa tttcagcttg
    18421 atggactttc atggtctctc tccttaacaa ggtgatgaat aaagcaagcg gctgtcgcaa
    18481 aagccaaaat gacttttacg gcagcctctt ttcatcaacc cgcttttttc tctgaatctt
    18541 tatagattac tgttggctca ccagacaatt cagctgcttc ttttgtgata atcacttttt
    18601 cgatgttttc atttgaaggg atatcaaaca tcacatccat catgatttct tcaatgatgg
    18661 aacgaagtcc tcgagcaccg gtattgcgtt cgattgcttt tttcgcaatg gctctcaacg
    18721 cttcaggttc gaattcaagt tcggtattat ctagagaaag aagtttttga tattgtttga
    18781 ctagcgcatt tttcggctca gtcaagatcc gtaccagatc atctgttgtc aatttctcta
    18841 aagctgccat aacaggtaat cgtccgataa actcagggat caatccgaat tttagtaaat
    18901 cttcaggaat gatatgctgc atgacacttt cgtcttcaga aagtttttgg ttttgtgtac
    18961 caaatccaat cgtcttttcg cccatacgat tcttcacaat cgtttcgatg ccatcaaatg
    19021 caccacctac gatgaacaat atgttcgtcg tatcaatctg aataaattct tgatgtggat
    19081 gtttacgtcc tccctgaggt ggcacgcttg caacagttcc ttctagaatt ttcagcaagg
    19141 cttgttggac accttcgccg gaaacgtcac gagtaataga tacattttcg cttttacgcg
    19201 caattttatc gatctcatca atatagatga tccctttttc tgcacgttcc acattgtaat
    19261 cagcagattg aagcaattta agcaaaatgt tttctacatc ttcccccaca taaccagctt
    19321 ctgtcaaact tgtcgcatct gcgattgcaa aaggtacatt caatgttttt gctaatgttt
    19381 gtgctaaaaa tgttttacca gaccctgtag gtccgatcaa gcagatattg cttttttgca
    19441 gttcaacatc atctgtgttt tcttgtgctt gctgattgac acgtttatag tgattgtata
    19501 cggcaacagc taacgagcgt tttgcgcgat cttgtccaat cacgtagtta ttcaatactt
    19561 caagaatttc tagtggttta gggacttcag tgaattcgcg tacagcttct tcataaaatt
    19621 cttcatcaat gatttcttta cataggtcga tacattcatt acagatgtag acccctggac
    19681 cagctacgat ctttttcact tcttcttggg ttttcccgca aaaagaacag cgtacggtct
    19741 cattgctact tggattgtca tacatggtct tcacccctta acttgcaaaa agtatgcaag
    19801 aaactatcaa agactttaca gtcttttact atcataacat agtttagtaa aaagaaaaag
    19861 tgtttaacaa aggatcaact gacctatacc tttgcatttt ttactcttaa caaaaaataa
    19921 ggaactgtga caaaatcgtg tcacagtccc ttatttcgaa taaacaattt tctagaagta
    19981 gcttcttcag aaatgattta ttacttgatt tctattcttt cgcttctgtc gctgtacctg
    20041 tgatcaattc gattgctttt ttcattgtca catcatgttc cagcatatct tctgtcaaca
    20101 cgcgtttgat ttgttcgatt ggcatgttgt atgtttctga taattcttgg atttctgcat
    20161 tgatttcttc ttctgttgct tgaagatttt cagcagctgc aattgcttcg atcacaagat
    20221 ttgttcttgt gcgcatttct gcttcacctt cgaattgttt atgcaagtct tcttctgtag
    20281 aaccagttaa ttgatagtac atttcaggag agataccttg acgttgcatg ttgttcaaga
    20341 attcgtccat tgaacgatgt acttcatcat gaaccatcac gtgtggcaat tcaacgattt
    20401 ctgcgttttc aacagccata cggatcgctg cttcatcttt tgcatcgtcc gctgcttttt
    20461 cttttgtttc tactaattct ttgcgatatt tttctttcaa ttcgtctaat gttgatactt
    20521 cgtcatcaac atcttttgcg aattcgtcat ctaattcagg cacttctttt gcttttactt
    20581 catggattgt cactttgaag actgcttctt ttccagcaag atcttctgct tgataatctt
    20641 ctgggaaagt tacatttaca tctagacttt cacctgattt atgaccaact aattgatctt
    20701 caaaacctgg aatgaatgag cctgaaccta attcaagtga gtaattttcg ccttttccgc
    20761 catcaaacgc ttcgccatct acaaagcctt cgaagtcgat tactactgta tcgccatttt
    20821 cagctgcagc atcttctttg ataactaatt cagcttgtgc ttcttgttca cgtttgatac
    20881 gtgcatccac gtcagcatct gttacttcac ggtcttgttt ttcaacaact agatctttat
    20941 attgtcctaa ttttacttct ggtttcactg ttacttcagc agtgattacc cagtcttgac
    21001 ctttttccat gctttcaaca tcgatttttg gttgagaaac aggatcgatg ccagcttctt
    21061 tgacagcagc ttcataagct tctggtaata cagcattcaa tgcatcttca tataaagctt
    21121 cttcaccgta catacggttg aatacttgac gagaaacttt ccctttacgg aatccaggta
    21181 cgttcaagct ttttttcact ttattaaacg cagttgttaa tcctttttgg atttcgtctt
    21241 gcgcaatcgt aaattttaaa acaccatcat tcgtgccttt tttttcaaat gttgcagtca
    21301 tttaattccc tccgagttta gaaattcatt ttatacatca aaaacgtttc atgcatacct
    21361 ttaaatagta cactaaccct ttcaaattgt aaacatattg aatggataga atccttgttt
    21421 ttcataacat ttttatcttt ttcactgata aaatctaatg gaagaagatc atttgctgca
    21481 aaattttttc tctttttttt aaaaaatctt gtttttcatc ttctgtacct tcatacatcg
    21541 tttttcctgt acacgcctct acagtcagtt gaaaccagcg cttcgggtct tcgatgtatg
    21601 tatcatagac aggataaaca attgcagatt gcaacgtgaa ttcttcattt aacacggaac
    21661 taagcagcgc atcttcatta ccgtaacgat ctgaaaatac acataattgc tggtaacttg
    21721 cctgctgctg cggctttggc agatcttttg gtatgacttt gactaacttt tcttcaatcg
    21781 tcaaatagaa aacaggatgg cattcgttca attgcctaag tgtctccagt aattttgccc
    21841 ttgctaaaat atggacctct ggcgatacaa taagtttact cgctgtttgg ataaactcat
    21901 gataaggaag ctgttcgatc tcatcaatag acgcaagctg acgtgcaggt tccaacgaag
    21961 gaagttcctc cgatttattg tggagtgcct tgatcttctg cctctccatt tgtccggata
    22021 attcttctgc aaattggatt ttttcaatga cccgctgctc gaaagcttca taaaaatctg
    22081 tacttgctat caattttcta gcatataaaa aatcttgtgt actgattaac aagtcaaaat
    22141 aaaattcagc aaactcctca ttttgaagat actgtttttc atgcaagagt gcttgcttca
    22201 atgcttcttt tttctcgtcc aactcgtaaa gacaaaaaac aagcaggcta ttagcctcaa
    22261 agctttcttt caactcgtaa gctcgttgaa ataattcttt tgcctgctga tattgctggt
    22321 ttgctagtgc ttgttgtgct actcgcatta aattctcata tttttctgga aaatcaatcg
    22381 gcttcacgca gtgtcctcct cttgctcatg ttcagctcat gttcaatagt agcaaaacaa
    22441 aaaaagcatg acaattaaaa ataactgaag caccctctaa aagctagatt ttagatctga
    22501 cttttagaag gtgctttcaa ctgttactgc tttttattgc tcatcagctg ttgacttcct
    22561 ggttgaatgc tttgatcaga ttgatcgtaa caacacttgt attagtgaaa aaatcgccta
    22621 gatgttgctt tttctgatta aagattgctg gaagataccc taacatcagc aaaggaaatt
    22681 ttagaatgaa ccggcaagca ccttctctaa ctaaaacggt tgaccagcta agttcttctt
    22741 catcaaagct gatgactcga ataccaaaga tcattttccc aatcgtttga ccatgattga
    22801 atttcgtcaa taaaataaaa tacgctagat aaataagtaa ggctgtgaat ccgtagatac
    22861 tcagataaga atctgaaaaa ttaagccaac ctagattttt ggcaatacca agtgttaaac
    22921 gcgtaatagc tccgatacac aaaagatcga ccaagaaagc aaacattcgg ataaaaaaac
    22981 ctgcatagaa atacttagga aaatcatttg cttttggcgg ttcatttctt gtgtccgtat
    23041 actttttcca tttacgttgt ttctttttga tttcttcggg cgtcaaaggc tctctttcaa
    23101 aagagctcag taccttttgg gaaaagttgg agtcaggtcc tacagcaggc tgctgttgct
    23161 tatctttttc ttgttcctta ttttcatttg aaagctctga catgcttact cacctccgta
    23221 ataatacatt gcttttggtg attccgctgt ccctagattc tcaagaacgg ataagatctg
    23281 actagtctca gaaggcttca atccctggaa ttctgctagt ttacttccta accatgtact
    23341 agcaaaccca gatggcgtgg aagaatattc tactagttcc gcatcttcaa gctgttgttc
    23401 ctttcgcata gcttttaaag catcttcagg aaatcccaat tcatcgacta aacctacttc
    23461 ttttgcttgg acaccatcat aaatccttcc atcagcaatt tttttgactt cttcttcaga
    23521 tttattccgt ccctcgctga cgattgctac aaaacgacta tatgcgctgt cgatatatgc
    23581 ttgaagcacc gcttcttctt cttttgtctc tggtcgcgtt cctgaaccca tatctttcaa
    23641 cgctccgctt ttaactgttg tatcttccac acccagtttt tctagaagtc ctgaataatt
    23701 caatcctgac atgatcacac caattgagcc ggtcactgtt tcttctgttg caaagatttt
    23761 atctgcttga gcagaaatat agtagccacc gctagctgcc atgtttttca tgctgacata
    23821 cattgggata tcatgttctt tacgaatcgt atccaatttc ttagcgattt ccgcactttc
    23881 gtagacaccg ccaccaggag aatttacttc taaaaagata cctttgactg ttggatcttc
    23941 ttcaatcgcc ttcagttgtt ccataaaatt tttatgattg taaccttcag aagcaaataa
    24001 acctgaagac cctcccgcag caatcgttcc atctactgat aattttacga ttttttgcgt
    24061 actgtctccc tcttccaaca catttgaagc tagttcttca tccccatata accaactgtt
    24121 caatccctca atttgatcgt tttcctgagg cttagtcaaa ctagctgaaa ctaacgaaac
    24181 cacaaataat cctgcagcaa tcagaactgc tgcccaacgt ctcttattca ttctttctcc
    24241 tttctaatac aagtttttta tgacgatcac agtttctgac tgctcagaag tgtcgaaccg
    24301 ctctgctttt tcttcccgcg catacccgga aaacgactct tttgtcaatt catcttctac
    24361 cgtaaactca cgagcttcag caacttccaa ggcatcaggt gcgatttctt ggatgctatc
    24421 ccagccagta tctaaaaata ttttcgtatc atctgataca ccagctgatg cttcgaattt
    24481 ggacaattgt ttttttaaca ttcccagtgt catgatctca atttttgaat ccatttttac
    24541 tcctcctcta tccgattact ttctattgta ccgaactcct gaaaaagatg atagataaaa
    24601 acccattatt ttttgacata aaaagagcat ttgacaaagc atttgatata taccaattta
    24661 ttttttattc tttttttgtt gacaagaaca ttcgcaaaag gttatgattt taaatgtttc
    24721 cagataaaaa acaaaagagg aggatagtta taccttttag gatagcgcca ggtctgtgta
    24781 aacagatgac gaggagaggg tttatcgaaa gattcggcgg atggccctag ggactgcact
    24841 ctacagaaaa tccacaaaaa atatctgcaa agataaaaca aaaggatttt cctgcagctg
    24901 gaagcattca tacgaattca ttatagaaag taggatgttt tttatgtgcg gaattgttgg
    24961 aatgattgga ttaaaaaatg taacaccagg attgattgac ggtctagaaa aattagaata
    25021 ccgtggttat gattcagctg gtatctttgt ctcagatggc acaactgatt atctggtcaa
    25081 agcacaaggc agaattcaaa atttgaaaaa caaaataaat attgatacta caggaaatat
    25141 aggtattggt catacacgct gggcaactca cgggcaacct tctgaagaaa acgcccatcc
    25201 acatacttcc caaagtggac gcttcgtgct cgttcacaat ggcgtaatcg aaaactttga
    25261 agaacttaaa attgcttatt tagcaaatga ccactttatt ggtgagaccg atactgagat
    25321 tatcgctcat ctaattgaaa ctttcgcgaa ggatactaca acgcaagagg cgtttttaaa
    25381 agctttgcgg gtcatcaaag gttcttacgc tttcgctttg attgatcgga cagcacctga
    25441 tgttatttac gtcgctaaaa ataaaagtcc actattaatc ggactcggcg atggattcaa
    25501 tgtgattgcc agcgatgcaa tggctatgct tgctcatacg aaggaattcg ttgagattga
    25561 agatgaagag atggttaccg tcacatcaga aaaagttatt attcaaaatt ttgcaggtga
    25621 catcgtagaa cgttcttcat tcgaagcaca agtagattct tcagatatcg agaaaggtac
    25681 ctatcctttt tatatgctga aagaaatcga cgaacagcct attatcatgc gtcgtatcgc
    25741 tcaaaaatac gtaacagaag acaatcgtgt ctctcttgat caaaagctgg tcgatacatt
    25801 gtctgatagc gatcgaatct atatcgttgc ttgcggaact agttaccatg cggggttagc
    25861 aggtaaacag actctggaaa aattgacaca gatccctgtt gaggttcatt tagccagtga
    25921 atttggctat aacacacctc ttctttcaaa aaaaccattc tttattttct tgagtcaaag
    25981 tggcgaaaca gcagatagcc gccaagttct ggtaaaaatc aaccgtctcg gctacccttc
    26041 acttacgatt acaaatgttg ctggttctac tctttcgcgg gaagcatcct ttacactgct
    26101 attgcatgct ggacccgaaa tcgcagtagc atcaacgaaa gcttatactg cacaaatagc
    26161 tgtactaact ttattagcca aagctatcgg tgataaaaaa gaatttgaaa cttctcttgt
    26221 attcgatatc actcatgaat tgagtttagt tgctaatgcg atggaatcgg tcatcgccca
    26281 aaaagattat ttagaagagt tggcagaagt atatctcagc gatacaagaa atgcctttta
    26341 cattggacgt ggcgctgact acaacgtttc tcttgaagct gctttgaaat tgaaagaaat
    26401 cagttatatc caggcagaag gttttgccgc tggcgaattg aaacatggaa cgattgcttt
    26461 gatcgaagaa ggaacaccag tgattggaat catcagtgaa gaagtgactg gtgcgcatac
    26521 tcgaggaaac ctgaaagaag tcgaaagccg tggcgctaaa acactagtta ttgtctcaga
    26581 gggattagaa aaggaaagtg accagctggt gctaccaact gtccaccctt atctaactac
    26641 tctaaccaca gtcgtaccga ctcaattact tgcatattac gcaacgctat tacggggatt
    26701 cgacgttgac aaaccacgta atttagcgaa atcagtgaca gtagaataat aaaagaacaa
    26761 ggaccagtgt cagacatctg acactggtcc ttgttctttt attttttatg aaaatatctc
    26821 ttaatccagt ccgatttctt ctttgactac tgccgcgatt ttatcgacat agtaattaac
    26881 tttctcatca gtaggcgctt ctgccatcac tcgaagtaat ggttctgtac cggaaggacg
    26941 aactaggatt cttccttctg atcccatttc agcttctgcc tcttcaatca cttttttgat
    27001 tgccggaaca tccatcgcac cgtttttatc acttactcgg atattcacta atttttgcgg
    27061 atagattgtt acctctgctg caagctctga taatttctta cctgtctgtt tcatgatatt
    27121 cagtaactga attcctgaaa gcatcccatc tccagttgtg ttaaaatcta agaaaatcat
    27181 gtgcccagac tgctcgccgc caaagttata atcgtttttg cgcatttctt ctacaacgta
    27241 acgatcacca acttgtgtaa cgacatcttt caaaccgatt ccttccaccg ctttgtggaa
    27301 tcctaagtta ctcatgacag tcgtaacgat cgtatctttt ttcaatcgat ttttttctgc
    27361 caagtatcta gcacagataa acatgatttt atcaccgtcg acaatgtttc caagttcatc
    27421 caccgcaata atacgatcgc catcgccatc gaaagctagt ccagcatctg ctcctttttc
    27481 aacgaccatc tcagctaaac gttctggatg ggtagagcct acgccatcat tgatatttaa
    27541 cccgtctgga tttgtgccca tggtataaaa atctgtttca agatcggcaa acaaacgatt
    27601 aacacttgtt gaagttgctc cgtttgctgc atcgacacaa atggtcaaac cggaaaggtc
    27661 accgttgatc gtttgtacaa ggaattgaga atatttcaat aagccttctg gaaattcttc
    27721 tacagttccc aatccttcag ctgatggacg aggcaattca tccacttcag catctaataa
    27781 tgcttcaatt tctagctctt ggtcatctac tagtttaaaa ccatctgcac cgaaaaattt
    27841 gattccatta tcttgtgctg gattatgaga agcagaaatc atgacccctg cgcttgcttt
    27901 ttgcaaacgt gtcagataag caacacctgg tgtagagatc acacctagct ggaacacttc
    27961 gatacccacc gataaaagtc ctgaaatcag cgcttcttct aacatttgac ctgagattct
    28021 tgtatcccgc cctactaaca cgcgtgggcg ttttccttct tcttcatgct ggcttaggac
    28081 ataacctccg caccgtccca gcttaaatgc aagttctggc gtaagttcct tgttggcttc
    28141 accgcgtacg ccgtctgttc caaaatattt acccatagtt gtaaaattac tcctttaact
    28201 taattttctg aggaatttgt tctgctcatt tgtgttgatt cgcttgtttc tgatgtttct
    28261 tctgtacttg tggtcgagct gcttgtccca gattccgaac tggaagaaat ggttgttgtc
    28321 gatggttccg ttcctgaatc agcagatgaa atacttctac tcgtacttgt attttcttgt
    28381 gtccgactgc tcgtcgaagc agagctactg cttgacgttt gcaacacggg tgtaatctcc
    28441 acactaactg attcgggctt gatacttaac ccctcttcta ctggtatttc aattttacga
    28501 gttgttcgat gattgatacc tgagacattg atcggtacag caatactgtc accgatcgat
    28561 gcaagcaaat cgctagagcc gcttacttct gcttccagtt ttgaaaaact gaaatcatag
    28621 ctttctaccc cggcagctgg tgttccttgc tgtatcccat aaagtcgtac cgacttgctt
    28681 ggcttgatcg aatcagctct cactgttact tgcactggat cagagacgat acttaatact
    28741 tcccctgctg cattcagcgc ctgtattggg acctcactat taatcccatc atctgtgacc
    28801 atcgaagcgt ccacatcagc tactactcgt ttgatttctg ccaatgtttt gtcacctgta
    28861 gtgatagaga cttttttagg actaacggaa gtatcatcaa gttcatatcc agatggtgtg
    28921 aacgttgaag gaatcaacgt ttccacatcg aaatctttta ttaccttttt ctcaaccgtt
    28981 actgtaatag tcgatggttc gatctttgct gtgacagcac tgctcaaatt ttgaattttt
    29041 agcggaacct catgtgttcc ttcgcccagc ttagtgatat ctgctgtgac gcggaacatc
    29101 cgtgtatctt cgtttgcctc tgcattcaac tgaactcggt ttgcactgga aagtttgaca
    29161 gtcactgcat tttcatatcc atggatatag tagcgctttg aatcataaga aatcttgatt
    29221 ggcaccccag taactgtttg attatacgct tcatttccag aaagcgtatt tggaacggac
    29281 tggccatttg cattgaagta cagcgcaata ctgaacagca atgcaataaa actgtaaaga
    29341 atgcttgtcc gtttctcttt tgagatcatt tgcgatcccc tcccttagtc ataccgtcta
    29401 ggaaagattg aatcacgttt ttcttgtttt ttgttttctc ttcagcaatc agttctctac
    29461 gcaaaattgc caaatattct tgctgggata agccagccat cagttcatta tctaatgtaa
    29521 tgctgacatc cccagtttct tcagagacaa cgatggtaat cgcgtcgctg acttcgctta
    29581 taccgacagc tgcccggtgt cgagtcccga attcttttgg aatcaacata ctttctgata
    29641 gaggaagata ggcgcttgct actgcgatct ttccttcttt tacaataact gcaccgtcat
    29701 gcagcggtgt attaggaata aagatattga tcagcagctc tccagtgata tctgcatcta
    29761 atgcaattcc tgtctcaata tactcatcta atccagtatg ccgttcgata gtaattagcg
    29821 caccgatttt tcgcttggac atatattgga tggctttgtc aaaagacaag atcatcttct
    29881 catcttcctg ctgttcgctt tttgattgtt tgaaaagcga actgcgtcca agatgttcca
    29941 gtcctctccg gatttccggc tgaaaaatca cgatagcagc tatcaccccg tacgtaatga
    30001 cttggttcat cagccaagac aaggtgtgca gaccaatgat ttccgctacg attcggataa
    30061 caataaaaat agctacacct ttcagaagct gaatcgcttt tgtgcctcgc agcagttgga
    30121 tcagtttgta tacaagatac cagacaacca gaatatcaag tatgttcacc aaaaaattca
    30181 gagagaacaa gtcaccggaa aacatttgct gccaatagtc caaattaaaa agttccccga
    30241 tttgaaatga cataaaaagc ctctcttctc ttttgagatt tcctctctag tatatcatat
    30301 gttttaattt tagaggctga tctgctcgta tttttcttga gaaaaacgca taattttcag
    30361 gaaaattgca agtatgcgtt ttatttttct ctgcaataaa tatttttaaa cttcagagaa
    30421 ttattcatta tattttttct gttatttttc ctttttttct tttgtagctt gttccatttt
    30481 tttacgtcgt tctgctctgg cagctcttct ttcttctgct tcaaggtccg tcgttttttc
    30541 ttttctagtc ttaacgggta catcgataga ttcctctggt tcaagtcgtt tcttgatttt
    30601 ttctaattct cccgtcagtg cagcatagtt gtaaaaacgt gttttagcgt cttggatagt
    30661 cttttcaaat aaagcttttg caagatttgg atttgcgttc tctagcgagg aaaagcgaac
    30721 ttgtttcctc atgaaatcag gcatagcaga gaaatcaggt tttttgtagt ccaaagtcat
    30781 tgggttcttg tttttttcga ccattgcagg gttatatcga tataatgacc aatagccaga
    30841 agaaacagct tcttttgctt ctttaagcga ttctttcatt ccaccaagca aaccatgtgt
    30901 gatacaaggc gtataggcaa tgattaaaga aggtccagga tacttttctg cttcttcaaa
    30961 agcctttatc gtctgcattt gattggcacc tgaagcaatt tgcgccacat aaacatttcc
    31021 atatgtcatc gccatcattc ctagatcttt ctttgaagca tactttccac tagccgaaaa
    31081 tttagcgatt gccgaagctg gtgttgcttt tgaggtttga ccgccagtat tggaatagac
    31141 ttcgttatcc aaaacaagaa tattcacgtc tgctccgctg gcaagtacat gatcgatccc
    31201 gccaaaacca atgtcgtaag cccatccgtc tccaccaatc atccattggc tgttttttac
    31261 gaatagatcc tttctcttgt atatatcctc cagttcaggg acaattgatc gttcgctttc
    31321 gatcgcagct tgcagttttg ctgctctttg ctgcgttcct tcgctctcaa acatgtgtac
    31381 caaccagtct tccatcaatt ctcgtagttc ttctgatact tttggcaata cttctttgat
    31441 tgaacttgcc aaagcctcgc gacgagtctg tgccgctaga agcataccat aaccaaattc
    31501 agcattatct tccaataaag aatttgacca tgcaggccct tgaccacatt cattggtcgt
    31561 ataaggagaa gcaggagaag caccgcccca aatagaagaa cagcctgtcg cattcgcgat
    31621 catcattcga tcaccgaaca tttgtgtcaa taattttaca taaggtgttt cgccacagcc
    31681 tgagcaagcg cccgagaatt caagaagcgg ttgattgaat tgtgtagaca agacagtatt
    31741 ctttcctttg actggatttg ctttttgtct taatgtcatt gcaaaagccc aattcacagc
    31801 ttgttctttc tgttcttcat aaggcttcat cactaaagct ttttcttttg cgggacaagc
    31861 ctctacacac agaccacaac ctgtacagtc ttccactgaa acttggatac ggtattttac
    31921 gccgtctgcg cctcgcattt ctctcacgat aaatccttct ggcgcttctt ccatttcctc
    31981 ttcatctgct aaaaatggac ggatggcagc atgaggacaa acaaaggcac attcattaca
    32041 catcgtacag cgatcactaa tccactcagg tacttctaaa gcgacgcccc gcttttccat
    32101 agaagccgtt cctaaaggca tttcgccagt tgtcattcca tttgctacaa gtgtaccaac
    32161 agagagttga tttccttcct gtgcattcac tggttcaagt atttcttgta tataaccagt
    32221 gttcctttta ttgatttttt caggcatctc caacgttctc caatggtctg ggatcgcgac
    32281 ttgatgaagc agttcggtcg tccgatcgat tgcttgtatg tttttttcta cgatagccat
    32341 tgatttatgt ccatagcttt tcagggcttc ttcttttagc agcggaagca cctcatcaaa
    32401 aggaatgatg tcagctaatt taaaaaaagc cgtttccatt gccgtattga tccggcgtcc
    32461 caaaccgact tcttgggcca atctcatggc attgatcgta tagaatctaa tgttattttc
    32521 agccaagtaa cgttttaatt ttaagggcag atgcgtttct aattgttcgt cgctccaaag
    32581 agtattcaat aaaaaaatgc cacctggctt caatcctttg accaagtcat agctatgcag
    32641 gtaagctggt gtgtgacagg caacaaggtc cgcatgctcg acaagatacg ctgaacgtat
    32701 cggcgtatct ccaaaacgaa gatgagagac agtcagaccg ccagattttt tcgaatcata
    32761 ataaaaatag ccttgcgcat atttatcggt gtgatctcca ataatcttga tagctgattt
    32821 atttgctcca actgttccgt cagatccaaa tccccaaaac ttggcctgga atgtttgcgg
    32881 ttctgtcaaa tccagacttt cctttggttc caaagattga taagtcacat catccacgat
    32941 ccctatggta aaccgttttt tgcgtatgct tgggtctttt ttcaattcat caaaaactgc
    33001 tgagatttgg tcgggagtca catctttaga ccccaatccg tagcgaccac caataacagc
    33061 cgggcgaagt tcagaatcat ataacgcact ttgaacgtct aataagagag gttctccacc
    33121 tgcccctggt tctttacttc gatcaagaac agcaattgtt tttactgtat gaggcaactt
    33181 ttgcacgaat gtttccactg gaaaaggacg ataaagatgg atattcaaaa aacctgtttt
    33241 ccgtccgttt gcattcaagt aatcaatcgt ttgttcaatc gtctgtgcaa ctgatcccat
    33301 agaaacaatg acttcttccg catcgtctgc tccgtaataa gtcaccaagt cataagaagt
    33361 tcctcgtaat cggttgatct ctttcatata tttttgaacg atttcaggta atttatcata
    33421 ataggggttg atcgtttccc gttgttgaaa atggatgtct ggattctggt tcgtaccact
    33481 aacagtcgga tgattgggat tcatccctcg tttacgaaat gaagctaatt tctcctgatt
    33541 gatcagagga gctaattctt catattccaa aacttcgatt ttttgaatct cgtgactcgt
    33601 acggaatcca tcaaagaaat tcaaaaaagg tacttctcct tcaatagaag caagatgggc
    33661 aacaggtgct aaatccatca cttcttgtac actgctttca gctaacatag caaatcctgt
    33721 ttgtcttgca gccatcacgt ctccgtgatc tccgaaaata ttcaacgcat ttgtcgtcac
    33781 tgcccgacta gcaacatgga aaacacttgg cagtagttcc ccagcaatct tgtacatatt
    33841 cggtatcatc aaaagcagcc cttgagaagc ggtatatgta gttgtcagtg caccagtttt
    33901 caatgatcca tggaccacac cagctgctcc tgcttcggat tgcatttcta ctacttttac
    33961 tggttgaccg aatacatttt cttttcctgc tgctgaccat tgatctacta attcagccat
    34021 ggtcgaactg ggtgttattg gatatattgc agcaatctcg gtaaatgcat aagaaatata
    34081 tgcggctgcc gtattgccat ccattgtttt catttttcgc atgattcatc atccttgttc
    34141 atattctctc ttattatctg ttccattata acgtaaaaca ttccattttt tcttttattt
    34201 ataaaaatat ttcacatatg tctatttgaa ttggtcatct aatttttaga caaacagtct
    34261 aaatcgttgc ctttttttgt cagacatgta taaactataa gcgaaagagg tgttttaaca
    34321 tggatatatc agtgattgat gcaacgaaaa caaatacaca aaaaggaatc ctttatggtt
    34381 catcgaatgc tcctaaaaaa atggtcgaat tcatcaacct atcttgtccg tattgccgac
    34441 aatggtttga agaatcttat gagctattag aagaagcagt ccaaagcggg caattgcagc
    34501 gagtgatcaa attgttcgat aaagaaaaag aaagcttgca gcgaggaaac gtgatgcacc
    34561 ggtatctcac aatcagcgat ggtcaaaaag cgatcaaaga gatcaaacaa atattcgata
    34621 ctcaagacga gtggaaacat ctttctctcc aagaagtagc ggattttgca gtagataaac
    34681 taaagctgac agaacaaaaa gacgaacagc tgagccaagc tgtcatcaac gaagctgagc
    34741 aagcacatat tcgatttgtt ccgactgtca tcttgggtaa agaaatcttt gatgaatcta
    34801 tttcaataga ggaattaaaa gaattgatcc aaacaaaata aaaaaataag gagcgtagga
    34861 caaaagtaaa gaacactttt gtctcacgct ctaaacacct ataaacggcg gaagcagaag
    34921 caactccttc ttatttcttg gagttaaacg cttctgtccc agcctcctta ttccgaataa
    34981 atggtgtcca aaagcttgca tctgccagat tatctcattt cttatgacaa gctgagcaaa
    35041 ttatctccac caaataattt attcatattt ttgacttgcg catactaact aattcttaaa
    35101 ttttcataca aaatttctca aattccctta acgagtaata tttatgtacg gtgattcaca
    35161 ctagtatccc aatcactttt taagttatcc cgatttcaat tctacttcaa aaatagttgc
    35221 ttttatgtat gctgttagtt gattagatgg tcgtttgttg agctaggtcg tttattttaa
    35281 acaagttgtt tctttagctt atacctatct cgtctaccaa acgcaagatg gcaaacacaa
    35341 atgcatacgc atctgtattt gccactttga ttcgtataaa gctaacaact cctaactcct
    35401 ctcatttaat agttgctctc ttttttattc agatacattt ttgctgatca cagcctagta
    35461 tttcaatggt tttgaacact aaaccaaatt gttccattac aacattcact ggctgatgaa
    35521 atggattaat agatttcgat tattcaaagc gattttctat tggtggattg tgttcccaac
    35581 taacaagacg gattccaaag tcattatata actcttcaag agttaaccct ttcctgttcc
    35641 caatagatgt atctctccca cgctcgatat aaaatatgga gtttcgaatg ccaagacctg
    35701 gcaaatcttt ccttgttaca gttacactgc tactaacttc cccatataaa atccagctcc
    35761 tccaaaatga taatatattt ttcgaccatc atctaactca tgtaacaata attgttgtgc
    35821 cctcttgtca ctcgccaaat gcaaacgcca cgtgcgatac catgccatgc tacctgcatc
    35881 tgcagaaaat ccatcaaata atgcaacaat tgtatcttct atcacaatcc tttccccatc
    35941 tacttcatat tctaatctaa aatcaaactc ccctcgggta atcgcaggtc ttggtggctt
    36001 aggacccaat attattctac ttaaaaccgc attcgttccc agatgaaaca cagctataac
    36061 aacaataatg attaatattt tgtttctatt tgataaattt ctcattattc gcaaatcacc
    36121 tctacattct cacatactag ttcgtaatac tcctttaata tcaaaatatc tactgatcgt
    36181 aataatattc gctatgaatt cctactactc gtataagaaa ggccaaatca tcagctttta
    36241 cttgctgatg atttggcttg tttctattta tcgtaattgt tttctaatca ccaaaattgt
    36301 taacaatcgg ttcagatatt tcccaattca ctagccttat attatattga tttaacaatt
    36361 cttcttcatt tattgtattt tgatcaagcg gaccaaattg ttctaataat tctttgtcat
    36421 gtaaaatatc ccttctagta atgcctcttt ccctttcaat gaaagaagca ctaggaaaag
    36481 tatgattata accattcaac ctatctccca tgtaataatt agcaggtcct ggaaaataaa
    36541 atatttgctt attatcacta acttctaaca gcaacactaa atcttctccg ctggctaaac
    36601 gacttgtcca tctacgatat ctccctatgc cttcgttcat tccaaatcca tcaaattcaa
    36661 ctattaacgt gtcttctatc acatgtaatt cttcattcaa ttcatactca agtctaaaag
    36721 gaaattctcc atatgtaatc tctggcaatg gtggtgctgg agttctccaa ataaatacaa
    36781 taactgtgaa tagaaaaaat ccaacgatcg atataaatgt tatgagacct gctcgaagta
    36841 aaacccgata taataaaaat gctgtatttc gctttgctat atctagaaaa ataaaaagta
    36901 ctgcaatcat aatgatagta aaagttgtaa aaaaacgcat ttttgccctc cctttataaa
    36961 catcgattct caaattctaa accaaatctt ggtagttcaa gttcaggtat aacgtattcc
    37021 aaccatacat ctcaattact atttatgaac ttatgattat cactgaataa atcactccat
    37081 ccccttgtta aaacttctac attaattaca cctggttgtt ttcgactagc taatccaatg
    37141 aacttatcaa ccatccttat ccaccaacca cgagaatctt gaccagaagt aagaagagca
    37201 gttctcaagg atgatcgagc agttccttcc agttttttaa ttaatcaggc cagttataga
    37261 taaatacttg atcttatcta tatcaggact tctatattaa gcaaaaattt atgtacaaaa
    37321 ctaaaatctt cattcagttg tctccccgct ttttatacga ttgtattaaa aacaatagaa
    37381 gcctttccaa attatgttac tccagtaaag atggaattat agtttgacgg aagaaaagca
    37441 agtataactt ttaaaaaaaa ctgatacatt cgcatctcct gcttcatgta tcagtttttt
    37501 ctttggatta aactgcttct tcacgcagtt tttccgcaaa ttcatttatt ttgcgaattc
    37561 ggtgattaat tcccgatttt gatatcgcac ctgagggaat catttcccct aattctttca
    37621 agctgacctc tggatgttga agccgaagtt ctgcgatttc ctgaagtttt tcaggaaggg
    37681 catgtagacc gacctttgct tcgatcaatt cgatgttttc gatttgttta gaagccgcat
    37741 caatcgtctt gttcagattt gctgtctcac aattgactag acgattgacc gaatttctca
    37801 tatcccgtac gattcgaaca tcttcaaatc ggagcattga gttggttgca ccaatcaatg
    37861 ttaagaaatc agcaattttt tctgctcctt ttaaataaga aatatagcca ttgcgccgtt
    37921 caagcgttcg agcatgtaga ccgtaataat ttaacatatc gcaaatgtct tggttatgct
    37981 gttcataaat agaaaatatt tccaaatgat aacggctcgt ttccggatta ttgactgaac
    38041 ctgaggccat aaaagcacca cgtaagtaag aacgcatttt ttgcgaattt cccataatct
    38101 cgtccgaaac gtgtccattg aacatcatac catccatgat atcaagatcc atcaacacat
    38161 cttttgtgcc ttgcttcaac cggacgatat agacattgtt cttttttaat ttcatttttt
    38221 tacgaacgag tagctcactt cgtacctgat aatgatcttt caaaagagaa tagatacgtc
    38281 tggcaattgc agcattttct gtttggacgt tcaaaataaa ctgctggttc acaataccta
    38341 aagagccgtt cattcgcagc aaagcagcta attccgcttt cgcatgttct cgatgaactt
    38401 ccaaaccagt tagttctttc ttcacatctg cagcaaatga catcctattc tcctcctttc
    38461 tccaacaata aattgcttaa tattttgaac cgaaaactaa acggaacaat tcttctacta
    38521 ctttgtctcc atcgtggaag acaccaccat ctttcaattc caaaaagtcg gtggaaatca
    38581 ctcgacatcc ttcatttctc aatccgttga agtcgtgtgc gacttgtact agatactcat
    38641 catagatctc tgggtccata taatcttcag gcactttttc cgtattgacc aatactgtat
    38701 cgataaaagg ctgattcaaa tgcttgtgta aaacacgtat atgatcggca tctgtgaaat
    38761 gttccgtttc ccctttttgg gtcatgatat tacagatata gacggtttca gcagttgtct
    38821 tgagcattgc ttcgccgatt tctggtatca ctaaatttgg aaggatactg gtgaataaac
    38881 ttccgggtcc caaaacgacc atgtcggcgt ccataattgc tgaaacgact tttcgagccg
    38941 ctttggcttc acttttatca tttgtgttgc agacatacac acggtcaatg gttttacggt
    39001 cgatcgcgat tttagattct cctactgcag tcgttccgtc tttaaagaca gcatgcaagg
    39061 tcaaaggagt ttctgaagaa ggataaacat gtccatctac atgcatcatt tttgctaata
    39121 attgaatcgc ctcataagtg cttccccgca tctcggaaac tgcggcaatg atcagattac
    39181 cgatcgtatg attggctaaa aactgatctt cctttttgaa acggtattgg aaaatatctt
    39241 catagaattg tggcatatcc gataacgcca ctaagacatt tctcaagtca ccaggaggag
    39301 ccatagcgat cgattctctg attgcaccgc tgcttccccc atcatctgca acagtcacta
    39361 ctgctgtgat atctgctcct tgatttcgca aacttttcag gataactggc agaccagtgc
    39421 caccgccgac cactacgatc tttggttttc ttatccggta ggttttcatt ttcacgaccg
    39481 gttcaccgtc tctttccttt tatccttgtc ccgatgtgtc acattgactt tatagttttt
    39541 ccctaaagca tttgccaaac gttcagtcaa tgccacagaa cggtgttgac cgcctgtaca
    39601 gccgattgca actgtcaagc tgctttttcc ttctttaatg tatccaggca atacagtttc
    39661 cagaagatcc aaaaatttat ggtagaatga ttgcgtttca tcaaaggaca tcacataatc
    39721 atagactggc tgatcttttc cggtcaatgg tcttagttcc gggatataat gagggttggg
    39781 caaaaaacgc acatccatca caatatccga atcgatcggc aacccgtatt taaaaccaaa
    39841 tgatacaaat tcgatccgaa atccttggtc tccctttgat ttgaatgact cattgatttt
    39901 ttcacgtaat tgtcgtggtg tcaatgtcgt cgtatcaatg atcacggaag cttgggcttt
    39961 caagtcgtct agtatcgccc gttctttacg tatcccttca gtgatcaaac cgtccatcgc
    40021 cattggatgt gctcgtcttg tttctttgta gcgagccact agttcttcgt ctgaagcatc
    40081 taaaaataag atagaagtgt cgatgaagtt cgtattttcg atttcgatca gcatgttttg
    40141 gatttcttca aagaaggaac gagaacgtaa atccactcct aaagccattt ttgtgacttt
    40201 gcctgattct ttgattaatt cccagaattt tggaatcaat cgaggcggca tattatcgat
    40261 acaaaaatag cccatatctt caaaactttg gatagctact gttttccctg caccgctcat
    40321 acctgttatg atgactaatt gcaaattatc tgtcataatt acgaactcct ctcacattgt
    40381 gcatagtaaa attatatcat tccgggcgta tcattaccag ttttcttttt cttttacaaa
    40441 aagatttgga ccaaatatct tttaggatat ttgccccaaa tccaattttt ttacttttcc
    40501 gatgctgtat ccaatgcctt gcttctttca gctagcgctc gttcaatcgt ccgatctgct
    40561 aaaatagatt gggcatcgat cactggataa tggttatctt cagcaaattc atgcatcaat
    40621 gacaattctg tgcaacctaa gataactttt tcacagttta ggcgctctac cgcttcttct
    40681 aagatttcgt aatatagctc ttgattcaaa taatccgatt ccttgatctc atgatagatc
    40741 agataattga ttttttcttg aagaacagta tcaggaatca ctgtttcgaa tccaagattt
    40801 ttgacttcgc gttcatagat tccagctttc atacttcctt ctgttcctag aatcgctact
    40861 ctccctgtcg tatgctgacg gacaagttca tttgctgctt ctcttggcat atgaaggata
    40921 ggaatatccg ttgccgcttg taattcttca aaaaagtagt gtgcggtgtt acaagtcaac
    40981 acaatgaagt tcggctgcaa taaattttgt ttctcaatat catccagcag aaacggcatt
    41041 ggattttctt ctgatcgatc taaaatataa gctgtacgat caggaactgt tgcatgattg
    41101 aataagacat aattcaaata ttcttgatct tttgtagctt tcgttcggtg gttgatcaag
    41161 cgaacaaaac tttctgtcgc catcgtgccc attccgccta aaatactgaa aaaattctcc
    41221 atgaactagc cttctttcac atgaaaatac tttttgaatc ttggaatata gttatggaac
    41281 atatatttca tcaacaacca gcgcttgatt gaccggtctt tttcataaaa cacagtagta
    41341 ccgtaacgat tttctttgat catttgctca gctagttttt tgtcttcatt ttcccgagca
    41401 tattctaaaa agattttttt gggcacaccc atccataatc ttgctttatc tgattgattc
    41461 gttccatagg tcgtttcaac aaagggtttg ttaaacactc gatcttctgt gacgaaacga
    41521 gccagattca agccgttcaa tgtcacaaag aaactgctac gaccttgtct caaattgatt
    41581 tcaaataatt tatattctcc atctctagga tcatacttca tatcgaaatt ggcaaagcct
    41641 gtatattcga ttttttccaa aaatgcctta atggtttgat agattttttc gttataatct
    41701 ggcatgatca caacgtaatt accaatcgat gctggtgtag gatcttctaa cagtggatga
    41761 ccaagacaca tcatccgaac ttgatgatct tcatctacat acgcatttag cacacgcata
    41821 ttactatcat cacccgggat aaaatcttgt acaatcattt cacttttata accggcttcg
    41881 tatatccggc ctaaaattaa atcgaactct tctctagtct ctaaaataaa agcttttttt
    41941 cttccttcaa attggacaga aagatactcc acactgtttg caggtttcaa agcaactggg
    42001 aattcaaaag gcaattcctg ttccaaatgt ccatttacta acatttcttc tctgacgatc
    42061 aatgttttag gatacggaag atcatattct tcacaaactt cataaaagct tactttattg
    42121 atcagtcttt caaataaaga ataatcaata tatggacaga taaaatattc tgacagctct
    42181 tgtttatgtt gggagatcaa ttctgcatat ccatcaccac aagcaatcaa aagatatttt
    42241 ttactcttat cagaatatct ttcttttgcc aaacgaagca tcgtctcgat aaacacaggg
    42301 tctttatcaa atcctggaat aacttctaca ttgacaattt tactgtagcg ggtaggggct
    42361 aactgatccg aagcatatgc ttggcagatt ttgccgtatg cttcattaaa agaacgtgcc
    42421 ataccataaa cgttcatgtc gcttcctaat aatataggga taaattcttc attttcaata
    42481 ctgttcatca ttatctctca cttactcata atctcttgtt tttcttttcc aactacacaa
    42541 ggaatatcat accataaaga aaaaagcaat caaaggaaac tcctccgatt gctcaattct
    42601 tttttgaacg gattgaactt ataggaagtt tttgtctata ccaatcagtg tagaaagaac
    42661 taccatgtaa tgattctaga acactgatat taataaaaca agcaaaaatt cattttttta
    42721 tgttttttta actatctcag ctatctatcc tctttatacc attatttttt tcaaatagtg
    42781 acctgtataa ctttctttta cgttaatgat ttcttctggt gttccagtcg caaggatcgt
    42841 tcctccacct tctccacctt ctggtcctaa atcaatgata taatctgctg ttttgattac
    42901 atctagattg tgttcaatca ctaaaactgt gttaccagca tctactaatc tttgtaatac
    42961 atgcaacaag cgggcgatgt catcgctatg aagtcctgtc gttggttcat ctagtatata
    43021 gaaattcttt ccattagaga ttttgtgcaa ttcactggca agtttcatcc gttgtgcctc
    43081 accaccggac aatgtcgttg ctggttgccc catcgtcaca taacctaagc caacatcaac
    43141 aatcgtttgc agtttgcgat gaatctttgg aatgtgcttg aagaattcta cagcatcttc
    43201 tactgtcatt tccaaaatat cagcaatgct ttttcctttg taatgcactt ctaatgtttc
    43261 agagttatat cgtttgccat gacatacttc acaaggaaca tagacatcag gcaaaaagtg
    43321 catttcgatc ttaataattc catccccgcg acaagcttca caacgaccgc cttttacgtt
    43381 gaaactaaaa cgccctttct tataaccccg catttttgcc tcgttcgttt gagcaaataa
    43441 cccacggata tcatcaaata cacttgtata agtcgctgga ttacttctcg gcgtccggcc
    43501 gattgggctt tgatcgatat cgatgatctt ttcgatactt tcgtagccgg aaattgtctt
    43561 gaatttacct ggcttagcag aattcttatt taatttttgc gctaacgatt tctttaagat
    43621 actattgacc aatgtactct ttccagaacc agacacacct gtgactgcta caaattctcc
    43681 taatggaaat tcaacactaa tatttttcag attgttttct gaagcaccag tgatcttgat
    43741 tgctttccca ttacctttac ggcgttcttt tggtacaggg attgattttt tccctgaaag
    43801 atatttccct gtcaaagaat tttcatcatt agctacttct tcaggagtgc ctgcagccac
    43861 gatttgtccg ccttgttctc cggccccagg tccgacgtca atcagataat ctgctgccca
    43921 catcgtatct tcatcatgtt cgactacaac gagggtgttg ccaagatcac gcattttctt
    43981 taatgagcca atcagtcgat cgttgtcgcg ttgatgcaat ccgatcgatg gttcatctaa
    44041 aatatacaaa actccagaaa gattcgatcc aatttgtgta gccaatctga ttcgctgcgc
    44101 ttctccccca gataatgttc cggaagcgcg actcaaagtt agatagtcta gtcccacatt
    44161 ttccaagaat gaaagtctgt catttacttc ttttaaaatc ggtttggcaa ttactttctc
    44221 ttgttcagat aacgttaagt ttgagaagaa atctactgct ttttgaatag acaactcatt
    44281 tgtttgcccg atattcgttc cattgatttg aacagataga gcttggggat tcaatcgata
    44341 acctttacat gtctgacaag ttaattctgt catataaaga cgcatttgct cacgcgtgaa
    44401 atcactgttc gtatcatgat aacgacgctc gatattcttc aatacccctt cgaaagtcgt
    44461 ttctacatcg cgaacgccac caaaatcatt ttcataatgg aaatggaaaa gatcatctgt
    44521 cccattcaag atgatatcct gatgctcttc cggtaattct tcgaatggtg tatctaaatc
    44581 gataccgaaa ctttcacaag cctgcgctaa catttgagga taatactgag agctgattgg
    44641 attccatgga acgatagcac cttgactaag tgtcttcgtt ctatctggaa tcactaaatc
    44701 ttgatcgact tctaatttga cacctaaacc atcacagtct ggacaagctc caaaaggcgc
    44761 attgaatgag aacagtcttg gttctagttc gccaacagta aatccgcaat aaggacaggc
    44821 atagtgttcg ctaaacaaca tttcttcttc accgatcacg tcaaccagag catagccgtc
    44881 tgccaatcgc agagccgcct ctaaagaatc aaatagacga gaacgaatcc cttctttcac
    44941 aatgatccgg tcgatgacga tcgcgatatc atgttttttg tttttctcca gctcaggcac
    45001 ttcacttaaa tcatagatct caccatcgac tcgaacgcgg acgtatccct ctttttggat
    45061 tcgttcgaat atttttttat gttgtccttt tttctttacc acgatcggtg ccaacacttg
    45121 gatctttgtg cgctcaggca aagccattac ttgatctgcc atttgatcta ctgattgact
    45181 ggtaatctcg atatgatcgt tgggacaaat cggatgtcct acacgtgcaa acaacaagcg
    45241 taaatagtca ttgatttccg tcacagtacc tactgtcgac cgaggatttt tacttgttgt
    45301 tttctgatca atagaaatcg ccggactcaa tccgtcgatg ctatctacgt ccggcttatc
    45361 catttgtcct aaaaactgac gagcataagc agaaaggctc tctacgtatc tccgctgccc
    45421 ttcagcgtac agcgtatcaa atgctaggga actttttcca gaaccggaca aacctgtaac
    45481 gaccacgaat ttatctcgtg gaatcgttac atctatattt ttcaagttat gggcacgtgc
    45541 accgtgaatc gtaattttat cgttagccat gttgtcctcc tactttgaag cttttaattc
    45601 taaaatcata tctcgcacat tagcggctgt ttcaaaatcc aatgctttcg ccgcattttt
    45661 catttctttt tctaatttca tcaataacgt atctttttct tctttcgtca tttcttcata
    45721 cgagacttgt accgcttctt ttgtatcatc ttccgattct ttcgtaatag aaatcaaatc
    45781 tcggatttct ttaatgatcg tttttggaac gatcccatgt tcttcattgt acttctgctg
    45841 aatcgtacgg cgtcttgatg tttcatccat cgctagacgc atagaatctg ttactttgtc
    45901 tgcgtacatg attactttac cttcttcatt ccgagcagct cgtccaatcg tttggactaa
    45961 ggaacgttca ctacgtaaaa agccttcttt atccgcatcc aagatagcaa caagggaaac
    46021 ttcaggtacg tctaatcctt cacgaagcaa attgattcca actaacacat caaattcacc
    46081 taatcgaaga tctcgaatga tttctgttcg ttccagcgtt ttgatatcac tatgcaaata
    46141 tttgactttg atccctaatt ctttgaaata atctgtgagg tcttctgcca tttttttcgt
    46201 caatgtcgtt acaaacaccc gctggtcttt ttcgactcgt tcatttattt cgcctactag
    46261 atcatcgatt tgtcccatga ttggacgaac ttcgatcact gggtcaagca gccctgtcgg
    46321 acggatgatt tgttggataa cggtatctgt ttgttcatgt tcatatggtc ctggcgtcgc
    46381 agagacataa atgatctgat tgacatgttt ttcaaactct tctaaccgaa gtggccgatt
    46441 gtctaaagca gatggcaaac ggaaaccgta atctactagc atctgttttc tcgctcggtc
    46501 accattatac attcctctga cttgaggcat ggttacgtgc gattcgtccg ccacgatcaa
    46561 aaaatcttca ggaaagaaat ccaatagcgt ataaggcggt tcaccttctt tacgtccgtc
    46621 catatgccga gaataatttt caattcccga cgtgtagccc atttctaaca tcatttcaat
    46681 atcgtaattg gttcgctgtt ctaaacgttg agcttccaga agtttgtttt catttcgcaa
    46741 gacagttaaa cgctgttcta attccgcttt gatattcgct acggcatgct ccatatgttc
    46801 atcattcgtc acaaagtgag tcgccgggaa gattgccacg tgttccgttt ctcctaaaac
    46861 ttcacctgtc agtgcattga cttctcggat acgttcgacc tcatctccaa aaaactccac
    46921 acgtaaggca cgttcatctc ttgaagccgg aaaaatctcc acgacatcac cgcgcactcg
    46981 aaaacgaccg cgctgaaaat ctatatcatt tctttcgaat tggatactga ctaagtcacg
    47041 aatcaactga ttgcgatcta gttctgctcc ctgtcgaatc gaaactactt gtttttggta
    47101 ctcaaaaggc gaacctaatc caaaaataca tgaaacggat gcaatgacga tcacatcatt
    47161 tctttctaat aatgaactag tagccgaatg tcgtaatttg tcgatctcat catttacact
    47221 tgaatctttt tcgatatacg tatcgcttga aggcacataa gcttcaggct gataataatc
    47281 ataataagaa acaaaatatt caacagcatt gttagggaaa aattccttga attccccgta
    47341 cagctgacca gccaatgttt tgttatgggc aataattagt gttggtttat tcactttctc
    47401 aataagattg gaaatcgtat atgttttccc tgttccagtc gcacctaata agatttgtgc
    47461 ttttttcccg ccgactacgc catccactaa ttggttaatc gcttcaggct gatcaccagc
    47521 cggttggtat ttcgagacaa gctcaaagtg tcgggaggta tctctctcaa tcattcttcg
    47581 tcctcctttt cattcattcc ttttcatttt acgaccaata aaaagaaaag gaaagcaaag
    47641 gcattcctta ctaaaaaatt ataccacacc gaacatactt tcgccacttt tttatcttat
    47701 tatgcatgtt acgttttata acataaaatg ttagaaactc gcattttttt cattttttcg
    47761 agcttacagt cttgtctgat tcagcacttt tcgcctataa taataaaata ttggaaattt
    47821 aggaggttga ttatgaaaag aaagcattta tttcactcat ttgttttctt aatgacagga
    47881 cttgtgacac ttgcgtttgg acatgttgca caagctgaca acactacaac ggcagagtca
    47941 agcacaagcg agacaatgca aaaaatagag cccaaaaaag acgtatacgt aattgccagt
    48001 gactctgctt ttgctccatt tgaatttcaa aacgcaaagg gcgagtacga aggcatcgat
    48061 gtaaaattga tggaaaacat cgccaaactg caaggattta acatccgaat ggactaccgt
    48121 ggtttctctg ctgctttgca agctttggaa tcaggacaag cagacggaat gattgccgga
    48181 atgactgtaa cagatgaacg aaagaaaagc tatgattttt ctgattctta ctttgatagc
    48241 gggatccaat tagctgtcaa aaaaggagat acttcgatca agtcttatga agatttgaaa
    48301 ggaaaaacag tcggagcaaa agttggtacc gaaagtgctg aatttttaag cgaccatgaa
    48361 tctgaatatg gctattctat caaatatttt gatgctgctg atcaattgta cgacgggtta
    48421 aaagttggcg caatcgatgc aatgatggat gattatccgg taatcggtta tgcgatcaaa
    48481 caaggacaag aattggaaac accgatcaaa aaagaatctg gcggaaaata tgcatttgcc
    48541 gttaaaaaag gacaaaatcc tgagcttctt aatatgttca atgaaggatt gaaagaatta
    48601 aaaagaacag gcgaatacga taagattatt aaagaatatg tagcggatgg ttcagaaact
    48661 tccagtgagt cttccactgc tgatgaatca acatttcttg gacttatcca aaacaactat
    48721 caagcattgt tgaaaggact ttggagaaca attctcctaa ctttgatctc ctttgctttg
    48781 gctatagtaa tcggtgtgat ttttggatta ttcagtgtag caccaatacg cgctcttcgc
    48841 acaattgcta gtatttatgt cgatatcatc cgaggaattc caatgatggt tcttgcgttc
    48901 tttattttct ttgggttgcc tggtatcgtt ggatttacga ttcctgactt ccttgctggt
    48961 gttatcacct tgactcttaa cgcaagtgct tatatcgcag agattgttcg aggcgggatc
    49021 aacgccgttc ctgtcggaca aatggaagct tccagaagtt taggtctttc ttataatcgg
    49081 acaatgcaaa aaatcatttt gccgcaagcg atacgcatca tgatcccatc attcgtcaat
    49141 cagttcgtta tctcattaaa agacacaacg atcatttccg caatcggtgt agtcgaattg
    49201 ctgcaaactg gtaagatcat tgttgcacgt acaacacaaa gtacgtatgt ttacttgatc
    49261 attgcgatca tgtacttgat tttgatcaca gcattgacaa aattagcaaa agttttagaa
    49321 aagaaggtga agtaagatgg cagaaaaaat cttagttgag catttagtca aaaaatacgg
    49381 cgataacaca gtattaaacg acatcaatgt gtccatcaac gaaggagatg tcgtctgtat
    49441 cattggccct tcaggttcag gaaaaagtac atttttgcgt tgcttgaatc gtttagaaga
    49501 accttctgac ggtgaaatta ttattgacgg cgcccacttg atggataaaa gcacagatat
    49561 caacttggtg agacaacata tcggaatggt attccagcac tttaacctat tccctcatct
    49621 ttctattttg gaaaacatca ttttagcacc gatggacctg aagaaagaat ctagagaaga
    49681 agcagagaaa aaagcaatca aattgctgca gacagttggt ctagaagata aaaaagatat
    49741 gtatcctgaa atgctttccg gtggacaaaa gcagcgtgtg gctattgctc gtgctctagc
    49801 gatgaaccct gacatcatgc tttttgatga gcctacatct gcactcgacc ccgaaatggt
    49861 cggtgatgtc ttgaacgtca tgaaaaagtt agcagaacaa ggtatgacga tggttatcgt
    49921 tacgcacgaa atgggcttcg ctaaagaagt ggccaatcgt gtcatgttca ttgacgacgg
    49981 aaacttttta gaagacggaa cgcctcaaca aatctttgaa aatccgcaaa atgaacggac
    50041 aaaagatttc ttgaataaag tattaaatat ttaataaaaa ccaaaaatgc ccggtcgctt
    50101 catgacgacc gggcattttt agtttcttgt tttttcactt aactgtacca gccgcatcgc
    50161 ttgttctcct gcacaaacaa gcaatcgctc tacctgttcc atcgcataag aaagtaccat
    50221 cggttcattg acgatatcaa gtacatagtc gataccacct tcagaaacta tgatgattat
    50281 cttattctaa agctggcttt tttgttggta caaacatgac aactacaaaa aaaccaggcg
    50341 aacgtatgat tgttcgtctg gtttttaatt attaatgact tagcgttttt actagaaata
    50401 gaaaatttgg gcaagtctac tttatatttt tactagaaaa actacttccg ctttttcccg
    50461 ccaaaagcat ctttaaactt atcgaagaaa ccgtcttctt gctgttcttc aactttgatg
    50521 ccgccagctt cagcgaatgc acgtaatgct tctctttgtt gttcatttag atttttcggt
    50581 gtgatcagtt ttactttgac atgttggtcg cctgttgcac caccacgtaa cttcggagcg
    50641 ccttttccac gtaaacggaa gttcgtacct gtctgtgttc cagcagggat cttcaatttc
    50701 acatctccat ggacagttgg tacttggact tcatccccca aagctgcttg tacaaaactt
    50761 aaaggcaatt catagtaaat ttcagaacct tcacgatcaa agatatcact ttcttctaca
    50821 cgaaaaacca catacaaatc accaaatggt ccgccgtttt ctccagcttc tccttgacca
    50881 gctaaacgca tttgttgtcc ttcttctaca ccagctggta cattcacttt aactgaatgt
    50941 gctttcttct catgtcctga gccatgacag gttggacaag gttctttgat ttctttccct
    51001 gtgccatgac aaacatcaca agtttgacgg ctcatcactc gtccaagtgg cgtttggcgt
    51061 tctacattga tcgtacctgc accatgacat ttatgacaag tagttggttg tgtgcctggt
    51121 tttgcaccgt ttccaccaca agtatgacag atttcttccc gattatactt gatttctttt
    51181 tctacaccaa aaatcgcttc ttcaaaagac aagtcgaccg tatattgcaa atcagcacct
    51241 tgacgaggcg catttggatc gactgaccga ccgcctcctc caaagaaaga gtcgaagata
    51301 tcttcaaaac caccgaagcc gccaccagag aagccgccga atccaccaaa tccgccacca
    51361 gctccaccag caccgtaatt tggatctgtg cctgcatgcc cgtattggtc gtatgcagcg
    51421 cgtttttgcg ggtcacttaa aatctcatat gcttctgata cttctttgaa tttttcttcc
    51481 gcatcaggtt ctttattgat gtcaggatgg tattttttcg aaagcttacg ataagctttc
    51541 tttatttcat cttctgatgc tccttttgag agccctaaga cttcataata atcacgtttt
    51601 gttgccatag gctttcctcc tattggttct catcattttc acgtcgcttg ttatcatatc
    51661 atattttgtt tgaaacaaaa accgatactt caaactattt ttcaaaagaa aaagccaaag
    51721 tagcaacttt ggccttctct cgaaattcag tgaactgatt atttatcgtc gccatcgact
    51781 tcttcaaaat cagcatcaac gacatcatct gctccgcctt gagctgcttc tggattttct
    51841 tgagcttgtt gttgtgcagc ttgttcgtat agtttaacag tcaagttttg aacgatttcg
    51901 ttcaatgcat cgcgtttagt tttcatttcc tcgatgttgt ttgcttcaac agcggctttt
    51961 aattcgtcac gagctgtttc tgcttttttc acttcttctt cgtccacttt gccttctaat
    52021 tctttcaatg ttttgtccac agagaatagc aatgcgtcta catcattacg taggtcaact
    52081 tcctctttac gagctttatc tgcttcagcg tttgcttctg catctttcac catacgttcg
    52141 atttcttcgt ctgttagacc tgaagaagat ttgatcgtga ttttttgttc tttttgtgta
    52201 cctagatctt tcgcagatac atttacaatc ccgtttttat caatatcgaa tgttacttcg
    52261 atttgtggaa taccacgagg agctgctgga atatctgtca attggaatct tcctagagtt
    52321 ttgttgtcag cagccattgg gcgttcacct tgtaatacat ggatgtctac tgctggttga
    52381 ttatctgccg cagttgagaa tacttgtgat ttacttgttg gaatcgttgt attgcggtca
    52441 atcaatttag tgaacactcc acccattgtt tcgataccta atgacaatgg tgtaacgtca
    52501 agtaaaacaa catctttcac atcaccagtg atcacgccac cttggatagc agcacccata
    52561 gcaactactt catcagggtt aactgatttg tttggttctt ttcctgtttc tttacgtact
    52621 gcttctacaa ctgcagggat acgagtagat ccaccgacta aaataacttc gtcgatttct
    52681 gattgtgaca gacctgcatc ttttaacgct tgacgtactg gtacttttgt acgttctact
    52741 aagtcagctg ttaattcatc aaattttgca cgtgtcaatg tcatttccaa atgcaatggt
    52801 ccagattctc ctgctgtgat aaatggcaag ctgatttgtg tgcttgatac gccagataaa
    52861 tcttttttcg ctttttcagc agcgtctttc aagcgttgta aagccatttt gtcttgagac
    52921 aagtcaatgc tgttttcttt tttgaattct gctaccatgt agtcgatgat tttgttatca
    52981 aagtcgtccc cacctagatg gttgtcgcca gctgttgaca atacatcaaa gacaccgtct
    53041 cctagttcaa ggatagatac gtcaaaagta ccgccaccaa ggtcgaatac taagattttt
    53101 tcgtcgcgat ctgttttatc taaaccatag gcaagagcag ctgcagttgg ttcattgacg
    53161 atacgttcta cttctagacc agcgatctta ccagcatctt ttgttgcttg acgttgtgca
    53221 tcattaaagt aagctggcac agtgataact gctttttcga ctttttcacc taaatagtct
    53281 tctgcgaatc ctttgatata ttgaaggatc attgctgaga tttcttgtgg tgtatatgat
    53341 ttaccttcta catctacttt gtaacctgct tcaccgatat ggcgtttgat tgatgaaatc
    53401 gtatttgggt ttgtcacagc ttgacgtttt gcaacttccc ctacttggat ttctccattt
    53461 ttgaatgata caacagaagg tgttgtacgg tttccttctg ggttagcgat gatttttgct
    53521 tcgtttcctt ccaatactgc tactgcagag ttagtcgtac ctaagtcaat accgataatt
    53581 ttactcataa ttaacgtctc cttctttaat tgaaagtgta agttttatat ccgcacaatt
    53641 attgtgcaac aatgaccata gttggtctta acacgcgatc atgtaatttg tagccttctt
    53701 gtagtacttc tacaatcgta tcggctggtg tatcttcagt cgctggaaca gtttgtactg
    53761 cttgatgaag attaggatca aaggcttctc ctttagcagg gattttttca attccttctt
    53821 cttccagtgc gttacgcaaa ctttccaaga ccatttccac gccttttttc aaacctgccc
    53881 cttgatcatc tgacacttct gtggctaatg cacgttccag gttgtcaatt gatggaagta
    53941 attttttggc taaatcttgt gaacggtatt tttgtaattg ctcacgctca ttttttcctc
    54001 gatttgccat atttgcgatt tcagcacgag cccgcaagta tttatcttcc atttcttcta
    54061 actcagcttt taatttcgct gtttcgaatt cttccgcttc agcttctgaa atacccgaat
    54121 cttctgcagc ttcgacatcg atttccggtt ctggctgagc atctgtcatt tctttatcta
    54181 gttcttcttg attttctttc acgatacagt cttcctttca ttattcagtc agctgtttga
    54241 ttgctcttac taagaaagag agaacgatca gctgcaaata gcttcgttat tcacattacg
    54301 agtttgacaa gtcaagcgag cggtagtaat cagcaagttt agctgctagt tctcttcgat
    54361 aaacatcgac tagaccgaac atccgagaat aaggcatact agtaggccct aacaaggcta
    54421 ttgtacctct tccatgacca tctatctcgt aacttgcttg aatcatactc atattctgca
    54481 ataaatcatt gcctaattca gcaccaatct tgatatgaat attgctatcc atcggagtga
    54541 tcatttgtgt aagtccgtct gagtctttca taaaagaaaa catcgatttg aactgatcaa
    54601 gatcttgacg gggctcataa tccaagagat tcatttgtcc actgacataa accttttctt
    54661 caaaaacatg accaagaacg gtgtcaaata actgagacat cccttctgtc gtctggaaat
    54721 atttatgaag aatcatcgga atctccgtac gaagtttatg gtacaccgtc atcagaggat
    54781 ctccgactag acggtcgttt acaatctgta ccattttttc tagttcctga cttcccatat
    54841 attcgggaag agtgaacact tggctttcta cattccccat atctgttacg acgatagcaa
    54901 taatctgtcg attattcaag ggtacgatac gaaagccagt cagtcggcgg tcttttattt
    54961 caggtcccaa tgaaaaagtc gtatagcttg tcagctcaga taatatctca gcagattgct
    55021 taataatctc attgatttca tgaaattctc tgcctagaga ctgacgaatc agcagcaaat
    55081 catcttctgc tacgcgagca ggttttaaca aatgatccac gtaataacga tagcctaaag
    55141 cagatggaat ccgcccagaa gaagagtgag tcttcaacag caatccttca tcttctaatg
    55201 ctttcatatc attgcgaatc gttgctgagc tggccttgac tccctcagac attaatgttt
    55261 tggaaccaac gggcacgcca ctactcgtat agtgttggat gatcagacgt aaaatgtcgt
    55321 tttgtcgctt tgttaacatc ttcctcacct ctcattagca ctcatatatt ctaagtgcta
    55381 attcacttat aaatatagca agaagccctt ctcctgtcaa gagaaagact ccatttttta
    55441 gcactctaaa cacaagagtg ctaattctaa ataattgatg taaatccagt tatatcaata
    55501 cttcaatacc tcattgtcaa actttctaaa ctttttcttt tcaagtttcg ataaaaaaca
    55561 aatattcttc aaactatttt tcaaataaaa agcgctcaaa cacattgttc cccaaaaaag
    55621 tccctcttgg tgtcaaataa acacggtctt cttcattaac aagtaaccct tcttgctcta
    55681 attcccttag cacatcaccg tacaccgatt caattgtttt ttgatagcga tcatagaaat
    55741 gttttttagt aatccctgtt ttttttctta gtcctaagaa catttcttct tctatttgat
    55801 tttttcgtgt caatttttct tcttccaata ccgggcgttc atttgctcgt aatgggcgta
    55861 aatagtgttg gattggtcct ttgttgcggt aacgaatatc tccaagataa ccgctagcac
    55921 cagctccaaa cccaaaatag tggtcattgt tccagtacat gaggttatgt tgagattcat
    55981 gtccggagag cccaaagtta cttatttcat attggtgacg tccatgcttt tccattgctt
    56041 caatcgtctc ttcaaacatt ctggtttccg tttcctgatc aggcagttcg agtcttcctt
    56101 gtcgtaccca gttcatgaac attgttttgt tttcgaggat caatgagtac aaagaatagt
    56161 gaggcagatc caaagccaaa gctttttcca aagtatcccg atatccttct agtgtttgtc
    56221 ccggtaatgc ataaatcaaa tcgatgctta cattggtaaa attttctgct tctaaaaatt
    56281 gcattgtttg atacacatct tctgcagtat gtttacgtcc aatttttttc aataagcgat
    56341 tgtcaaatgt ctgcactccc atggataaac gattgacgcc atagtttttc atcacttgaa
    56401 gtttttcacg ggtcaaatca ccaggattcg cttctacagt aaactcatta ccattttgaa
    56461 aaggcaggat ctctctagca cctgataata gtcgatcaag ttgtttagca gataaagaag
    56521 ttggtgttcc gcctcctaca taaagtgttt ccaaattcgc tgtaggatga ctttccagca
    56581 ttaatctcat ttcattcagc agcatttcca catactcatc tactggctgc ccttctaaaa
    56641 aaactttatt aaagtcacag taatagcaga tatgctcgca aaatgggata tgaatgtacg
    56701 cagaaacctt gttttctgcg ggatttattg tttctatcat tgaaattctt tctccttacg
    56761 tcgaaaagtc gtgcctgttt tcgaccttct taaaatcgcg ttcaagcacc aactcattga
    56821 aataagccgc atatttagaa tcaaggaaac ctagctctaa aaatgcttgc ttattgtcat
    56881 cggagctaca cggtgctttt acagcctctc gataaacggt aatcgcaaag ctgaccttcg
    56941 gcattaagtc aaaatctgca aaaatatgaa aagctatttt cacagattcc tccttaagtg
    57001 ctcagaccac aacgctttgc ttacaacctc tcatatacgc atactaaacg tatttggcga
    57061 acaaatccac taaaaaatcc aagaatcgac ttcttggatg atttttttac actcatgatt
    57121 cttcctaaac aattttgtcc ttattttatt cataaaaaaa tctagtcaaa tctctcactt
    57181 caagagtttt aactagattc caattattat ttttttgtaa aaataaacag tctcttaatc
    57241 atagacattt cttcggtgcc ctacttgaat agcgataaca acgaattgat tatcttctat
    57301 ctctaccaat acacgtattt gccaatcaga gctgcttctg aatttcgggc gtgaatttta
    57361 aacggcaggt cattccagcc caccaaattc attaaggatt cttccatgaa tactgttgtt
    57421 ttcccacttc ttatccaatg tttatgggca atttcagccg tttgacgatt gtattcatct
    57481 tctagttgtt ccatcgaata gttttttaga agttcagata aagtaatccc ataaaattca
    57541 gccatataag aaagccattc ttttttggcg tcacttacac gaaccgtaat catagcccta
    57601 taattgcctc tcttaaagaa tctcatgcat ttcctgatgt gattttagaa aaatttactt
    57661 gctaaaaaac tgtgaaaatg taggagctag catccttgta gtaaatgatc gagctcttta
    57721 ttatacatta aaaaagttca gaaattttga tgaagtagcc aactactttt tcatgagtaa
    57781 agcaacacta gaaaaagaat cctccagtac ttagaattta ctggaggatg tacttttgaa
    57841 gaagcctata ggttatttaa aaaatattac tacaggaagg aacgagaatt acttcgttac
    57901 ttataataga acttagtctt atatagaagg catagctcac attagcttat ttgtcctata
    57961 aaaataaatt acggtatttt agaaaagcta ttttctcttt tttcaacgat cttcagcaat
    58021 ttttctttat cgtcaggtaa ctcaagttca tgaattctag catgacaatt gggacataac
    58081 gcgatacagt tttcaatcac atcttttcca ccttgcgaca aatattctat atgatgacaa
    58141 tgtaaataag gtacaccgtc ttttataaat ggcccttttt cttggcaaag ttgacaaata
    58201 ccttttgcaa gtcgtttaac atattcagat acgaagggat tacgtttata tatttttgtt
    58261 tttgagaagc aaaactcctc ttcgttaaca tattttgcag ccattttttt attataatta
    58321 ctaacttttc ttgcttcttc ctctaattta gcattagata aattacgaat atgtctagtt
    58381 tgattttctt cctggttata tatctttatc ttcaatcaat tgctcttgat ttattaaagc
    58441 taatggaaat ttatatactt ttctgctgtt gcccgaacta tctttttcat taacagtata
    58501 tatttgtcct gctaaatata cttctcccag atatgtgtgt tcttttggtt taaatgtttc
    58561 aaataaatga atctttacaa aattactttt tgaattggct aaaactttat tttgattctt
    58621 atccaatact tgatcccctt ttagccccat accggtatat tgtaaaatac catttaacca
    58681 cttatcttta taaacattac ttcctgtttg atcactaaac agtacaagag tattagtttg
    58741 atgagatctt ctcattccac cctgtttgga acatttaaac ttctctgaaa tttctttgtt
    58801 agtatatact ctaccaactt ctaactcacc taatatgttt ttagaccaat tttctcgcca
    58861 tgtaataatc ttatcaagat taaacatttc tccctctttt ggcatgccat ttctaattaa
    58921 attagctaat acaccagtag aaatatctaa tttttttagt aattctttct tagtaatcaa
    58981 tgttggattc ataacaatgc agctcctaat gatatcaata taattgcatt agataacttg
    59041 aaaaatcgaa aaaaactcat tttgtgtcaa gttatctgta atatataaaa atatcattat
    59101 tatacctcat ctatgtgaat ttttactatt cttaccttag catattcttt ataggtatat
    59161 cagagctaat caaactaaaa aatacccgaa gtcttcttat gtaaaaacac cggttattac
    59221 ttaaatatga cttaggtaaa acaatgagtg atccaattat aaaattttat ggcgaataat
    59281 ttagtaagcg ctacatattg aatgatttag aaattttgaa aaagtagcta gttcctttta
    59341 taccagaaaa gtaaacctaa taaaaaacac aactaatttt tagctgtgtt
//
LOCUS       DAZKKW010000010        52004 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.010, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000010 DAZKKW010000000
VERSION     DAZKKW010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 52004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52004
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.010"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            25..696
                     /locus_tag="ACG8PO_000563"
     CDS             25..696
                     /locus_tag="ACG8PO_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFQ0205916.1"
                     /translation="MNFLVVLKDTLIERSTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSI
                     QFPQLITMIHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVA
                     MEKAN"
     gene            755..1195
                     /locus_tag="ACG8PO_000564"
     CDS             755..1195
                     /locus_tag="ACG8PO_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0205917.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            1207..1875
                     /locus_tag="ACG8PO_000565"
     CDS             1207..1875
                     /locus_tag="ACG8PO_000565"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFQ0205918.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            1939..2562
                     /locus_tag="ACG8PO_000566"
     CDS             1939..2562
                     /locus_tag="ACG8PO_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HFQ0205919.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            2618..3004
                     /locus_tag="ACG8PO_000567"
     CDS             2618..3004
                     /locus_tag="ACG8PO_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HFQ0205920.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            3217..4608
                     /locus_tag="ACG8PO_000568"
     CDS             3217..4608
                     /locus_tag="ACG8PO_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HFQ0205921.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
     gene            5219..5587
                     /locus_tag="ACG8PO_000569"
     CDS             5219..5587
                     /locus_tag="ACG8PO_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HFQ0205922.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            5767..6708
                     /locus_tag="ACG8PO_000570"
     CDS             5767..6708
                     /locus_tag="ACG8PO_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HFQ0205923.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            6777..7508
                     /locus_tag="ACG8PO_000571"
     CDS             6777..7508
                     /locus_tag="ACG8PO_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFQ0205924.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            7652..9832
                     /locus_tag="ACG8PO_000572"
     CDS             7652..9832
                     /locus_tag="ACG8PO_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFQ0205925.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            9852..10334
                     /locus_tag="ACG8PO_000573"
     CDS             9852..10334
                     /locus_tag="ACG8PO_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HFQ0205926.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            10350..11111
                     /locus_tag="ACG8PO_000574"
     CDS             10350..11111
                     /locus_tag="ACG8PO_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFQ0205927.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            11242..12576
                     /gene="murC"
                     /locus_tag="ACG8PO_000575"
     CDS             11242..12576
                     /gene="murC"
                     /locus_tag="ACG8PO_000575"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFQ0205928.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            12883..13368
                     /locus_tag="ACG8PO_000576"
     CDS             12883..13368
                     /locus_tag="ACG8PO_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HFQ0205929.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            13361..14044
                     /locus_tag="ACG8PO_000577"
     CDS             13361..14044
                     /locus_tag="ACG8PO_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFQ0205930.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            14170..16815
                     /gene="polA"
                     /locus_tag="ACG8PO_000578"
     CDS             14170..16815
                     /gene="polA"
                     /locus_tag="ACG8PO_000578"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFQ0205931.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            16860..17696
                     /gene="mutM"
                     /locus_tag="ACG8PO_000579"
     CDS             16860..17696
                     /gene="mutM"
                     /locus_tag="ACG8PO_000579"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HFQ0205932.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            17660..18289
                     /gene="coaE"
                     /locus_tag="ACG8PO_000580"
     CDS             17660..18289
                     /gene="coaE"
                     /locus_tag="ACG8PO_000580"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFQ0205933.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEKLVGQIENWLFANRFL"
     gene            18341..18673
                     /locus_tag="ACG8PO_000581"
     CDS             18341..18673
                     /locus_tag="ACG8PO_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFQ0205934.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            18985..19476
                     /gene="nrdR"
                     /locus_tag="ACG8PO_000582"
     CDS             18985..19476
                     /gene="nrdR"
                     /locus_tag="ACG8PO_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFQ0205935.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            19499..20872
                     /locus_tag="ACG8PO_000583"
     CDS             19499..20872
                     /locus_tag="ACG8PO_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HFQ0205936.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            20872..21798
                     /gene="dnaI"
                     /locus_tag="ACG8PO_000584"
     CDS             20872..21798
                     /gene="dnaI"
                     /locus_tag="ACG8PO_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HFQ0205937.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            21927..22367
                     /locus_tag="ACG8PO_000585"
     CDS             21927..22367
                     /locus_tag="ACG8PO_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFQ0205938.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            22511..23212
                     /locus_tag="ACG8PO_000586"
     CDS             22511..23212
                     /locus_tag="ACG8PO_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine(22)-N(1))-methyltransferase TrmK"
                     /protein_id="HFQ0205939.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            23209..24330
                     /locus_tag="ACG8PO_000587"
     CDS             23209..24330
                     /locus_tag="ACG8PO_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HFQ0205940.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            24345..25577
                     /gene="pepT"
                     /locus_tag="ACG8PO_000588"
     CDS             24345..25577
                     /gene="pepT"
                     /locus_tag="ACG8PO_000588"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HFQ0205941.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            25709..26617
                     /locus_tag="ACG8PO_000589"
     CDS             25709..26617
                     /locus_tag="ACG8PO_000589"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFQ0205942.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            26652..26924
                     /locus_tag="ACG8PO_000590"
     CDS             26652..26924
                     /locus_tag="ACG8PO_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HFQ0205943.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            26935..27981
                     /locus_tag="ACG8PO_000591"
     CDS             26935..27981
                     /locus_tag="ACG8PO_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhN family protein"
                     /protein_id="HFQ0205944.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            28232..30841
                     /gene="clpB"
                     /locus_tag="ACG8PO_000592"
     CDS             28232..30841
                     /gene="clpB"
                     /locus_tag="ACG8PO_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFQ0205945.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLDSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(30992..31678)
                     /locus_tag="ACG8PO_000593"
     CDS             complement(30992..31678)
                     /locus_tag="ACG8PO_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0205946.1"
                     /translation="MEGLSVIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGIT
                     FIVESLLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWD
                     KILHAVSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLC
                     DQFLGMNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFE
                     SYKLEKVKNT"
     gene            complement(31656..32150)
                     /locus_tag="ACG8PO_000594"
     CDS             complement(31656..32150)
                     /locus_tag="ACG8PO_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HFQ0205947.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(32170..33390)
                     /locus_tag="ACG8PO_000595"
     CDS             complement(32170..33390)
                     /locus_tag="ACG8PO_000595"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0205948.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            33537..35372
                     /gene="lepA"
                     /locus_tag="ACG8PO_000596"
     CDS             33537..35372
                     /gene="lepA"
                     /locus_tag="ACG8PO_000596"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFQ0205949.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEQDSVK"
     gene            35568..36479
                     /locus_tag="ACG8PO_000597"
     CDS             35568..36479
                     /locus_tag="ACG8PO_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0205950.1"
                     /translation="MVDNSSGRVLKSLRKEKKLSQKKLADLAGISQSTLVKYEKGSRK
                     IPKDVDNTLSKILNIETLIKDEEDKIEILIGKLIAYRDMNKLLNKELADNIGISEVLL
                     SYVLNRKRNPSKEMQKKIDIFLLSNEKEILKEINRDSEIFSLSKDDKIVMGKRIREVR
                     KNREETLEKFGKNFTIYTGKNVISRWEKGINIPDIEKLMNIAYLGKVTVPYLMYGEDY
                     KNILPKDERVSDFKKINSFSMGLRMRKIRKDYYLEREEFGKLFSPSISKWSIDRYENG
                     RDIPNTNRIIQYAYIGNLSLEFLIYGI"
     gene            37490..37726
                     /locus_tag="ACG8PO_000598"
     CDS             37490..37726
                     /locus_tag="ACG8PO_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0205951.1"
                     /translation="MFNTRGQSFLGIEGTRVTVDGWCDCFEVGKHVELMAFTELKDKN
                     NVEFFESDIGWDDHEEVYGKFILRKGNSYTNGEA"
     gene            37839..38081
                     /locus_tag="ACG8PO_000599"
                     /pseudo
     CDS             37839..38081
                     /locus_tag="ACG8PO_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010708076.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            38171..38410
                     /locus_tag="ACG8PO_000600"
     CDS             38171..38410
                     /locus_tag="ACG8PO_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0205952.1"
                     /translation="MIDISLPEDLKPDPVTIGNSVMIFDSLDEAMSYGWKEIKDEGSN
                     WYGMRFGDGEVAKKAGIPIGKWTVDFHEAGSFFVL"
     gene            38557..42840
                     /locus_tag="ACG8PO_000601"
     CDS             38557..42840
                     /locus_tag="ACG8PO_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VaFE repeat-containing surface-anchored protein"
                     /protein_id="HFQ0205953.1"
                     /translation="MYAKTKMNWLKALALVAFSQFCFLVGTAQAHAEETTAIQPTMEA
                     IAQVASEVTEVQPAEAGVPAGEATVEQAPVTEAPTVNSKTTLDQTIGETQHPGNSPGT
                     SQPVEETASITTPASETSTLEGEKAAEATPSVQPESSEKQAEEKKRIEETVEQPAATA
                     DKEGKNEENVEKTISSTAASQNQPTKPNKESTASIENQKKKEQNIPKTGTPTLLVTVP
                     TNRSVRVRRATNAAVLGGRKIDWGENIGVTSSPNNLNFTLNYMVRFEINGQVAFCIEP
                     GVSAAEGQVYTATALKNYLKNSNVRKKTSLISYLGYVTNKDKSDEQYIAAQFMIWEAL
                     GTKIPIKNIRVDYDRRKAEIQRLINNFGEKATFNNQTHTVKIGETLKVKNKNDFLKYV
                     KDIQKPKGVNVSIHGNEVWITVTKEAPDKAKIQLNKIKVQGAPLAYRRPGAQTIGVLH
                     PTEPNASVINLQILKQGNIRVFKEDAETGVKAQGAASLENAVYGLYQADGKKIKEITL
                     KKINGKVQAEIKDLDPGKYVLKEIKAPKGYVLSDEAIEIVVEPGKTVNATAKDQVIKG
                     TIDLVKVANKDLVDSTNPDNKPKLAGIEITLTSKTTGKVVKTVVTDNDGYATFGKNTV
                     VFDTYILSETKGKEGYKLFEPLEVTISENGQTFHYVLEDKVIEQRLKVVKVDQETGKV
                     IPLANTEFKIFDTLANRYVTMEIPNDTETTDIFKTNEKGFFLTNGTLTYGKDRYRLEE
                     IKAPVNYVLNQNPLVFSITNDTEPIKVIHFANRQARKSVKLFKYEEWNKKKTPLAGVN
                     FTLYNGKNQAIGEYTTDQNGEILVKGLVADDYYFLENAPLESFQPNTDKQAFTVAFKQ
                     NAPDDGETLVVSVRNYLIPPKLETQASNKADGEKVVDPLETIVIKDSVSYTNLFVGKE
                     YTISGVLMNKATNAPILQDGKEITSTLAFIADKVNGTKDLEFVVNASVLKGQTIVVFE
                     KLFRDGIEVAAHADINDEAQSVKVNNPTVHTTAVFEDGLTVADPTGLFTLNDTVEITE
                     ILPGKAYLVKGILMDKATGEPLLVHGNTVETSVGFIAKAENETVIVPFTFDLTGINGR
                     EIVVFESLFRNDEELANHKDINDKGQTVRVTNPSVRTTATHKPTGLKEVNPLTKVTIT
                     DAVKYHDLVVGKTYTVNGVLMDKVTEKPVLVDGKEVTSTLTFVAETTDGTVNLDFILD
                     ARALRGKEVVVFEDLFREGVLLASHADITDNDQTIKISNPTITTKATNKNGGKEILAL
                     PNQTVLEWVKVDGLAIGQIYRLIVQGYLTPDGTPFEGTHAEKVFAADKEMMEFLFEFL
                     VDGRNLAGKGLSFAEWLQAKTEDEKETFEEVAKHNVDLTNKDQTVQIVEAPKPPQPVK
                     PEQRVKPATVVPMLPKTGEQTSSILMVLGLVVLVIALGITLYKVRKAKD"
     gene            42939..43304
                     /locus_tag="ACG8PO_000602"
     CDS             42939..43304
                     /locus_tag="ACG8PO_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0205954.1"
                     /translation="MAEKALRMKKEFLGQVFLLGSREPNPPKEINGVSGRYVQLQAEC
                     SREEQPAFFVYLTAEEDKRLRLPEKEVRISFEEAYFVFRKERVNSRETNDMGQTERRT
                     TGYQDVGVFLTGVKIGEVL"
     gene            43306..43758
                     /locus_tag="ACG8PO_000603"
     CDS             43306..43758
                     /locus_tag="ACG8PO_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0205955.1"
                     /translation="MGLKFPNNYDITEALDETCLGAVRFGALEESDRVFDNETGDYTG
                     EIRSQRAQLVFGNPDEPKIARRVKFPADVDLTGFKRRDPVKLIDPEYHVVYIPAQDGN
                     REYSDLVIYAKGLEKVEAPTNNPNMKQSQEKGLDQKQPSVDSKEKEKK"
     gene            44207..45682
                     /locus_tag="ACG8PO_000604"
     CDS             44207..45682
                     /locus_tag="ACG8PO_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HFQ0205956.1"
                     /translation="MKAHFFKYRGRRIRYFNRYLWFCYYGFFLFPFLILLGWFSWKVF
                     YPRLSTFSNWQMYLIPGISIVLYLLLVSGLILGLMHWSRLKEGYFASVYWRQLLVRML
                     IDNRFVYTKKQVSEKQTREKMRLPKVYFYQKKQELVVSFPLDGGRFHERFLKMGHLLS
                     EVFLRDLIREEREKARINYVFLSDSGRIPIDQCIAENGRIHLMKTLDWVYDQSPNMLI
                     AGAIGGGKTYLLYSLIQACLSVGTVDICDGKAADLAALGDVDVFKGHVFYKINEEMII
                     CLRNALKEMNQRYAYMKTMKDPKYEPGKNYAYYGLPPHFIFFDEWAAFYGSLDMEARN
                     RVDKLIQPLVLKGRQAGMYIILAMQRPDAEYFPSGVRDNLTFRISVGRLSPIGYLMTF
                     GEDFKNKPFYNKDQPGRGYAFPGFDVPVEFFAPFVYKDYHFIKEFEKFTEMIVYDFTV
                     KEDSQDQTMGDDNQLLQEQLERQMYLAKDPKGTSFSDAHIL"
     gene            45705..46016
                     /locus_tag="ACG8PO_000605"
     CDS             45705..46016
                     /locus_tag="ACG8PO_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0205957.1"
                     /translation="MAESCYYRVATAIRMINPSLSSRTFYDWLNRIEQVTDYRFLRKE
                     RVFTGKVINQVLLTKKDIERLTRLYHYRVDLEEDLTLSIYRVFSPEKYSEITKLDHLI
                     L"
     gene            46377..47633
                     /locus_tag="ACG8PO_000606"
     CDS             46377..47633
                     /locus_tag="ACG8PO_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFQ0205958.1"
                     /translation="MTPIELKSYRKKLSAIHNISGDKKPLSQARFAQLMGCSKDYIRK
                     MEQGKRKIPEDFEQRIKERFRLYMKSGIDLRAMIDYLRLSFFGTDYKTVIQRVLRMDL
                     TEFETRPTGLYLFDCLSVRGNIWVFWHSKRTVTENVLVQFSGQGCREYEEVMREQQTD
                     WRTWLYSIWKNQGKLEAGYTRVQCSRFDIALDELWGLETEEHFDLFELLEKQKAGLLE
                     MDFKRFQNIGGCFLDDGYFRSEGLSLYFGSRKSPLFFNFYEKRFEIANREKISELEAL
                     TKYGIYNRYELRLANEKATQAVEAFILGDSPKRLGEIGVGLINAKLIVWDRLESDARI
                     VDKRWAKLFRSMEELDFTVKPEKFSLDRSERWFLKQVAPTLKLLQEADRILGRDFVQE
                     GIDNAELNDEQQNYLEWMGTSGGKIA"
     gene            47630..48175
                     /locus_tag="ACG8PO_000607"
     CDS             47630..48175
                     /locus_tag="ACG8PO_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0205959.1"
                     /translation="MNAYTTTEWLRLLDLKAAIEALNEKMVDLSYFRFRVPYIEQAVK
                     AGRYQEKENWQEIARLLEVRKGYEQELEELEFSRRKGRLEFIRFYRFSLPIPAILAVK
                     KGCDKMKIYENCVAALSSEKPLMEEISLVTVTWEMSRQPTEEQTYLSLEEIEIELEEI
                     GRYATCSTYCGSVISIAGVIV"
     gene            48176..48676
                     /locus_tag="ACG8PO_000608"
     CDS             48176..48676
                     /locus_tag="ACG8PO_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0205960.1"
                     /translation="MKKIMIYVGAYWSRDPTVLENPEAVHYCLRQLFYLYKERLESLI
                     RQLPYTDRRLDELLLRYPAMYKRRKNRLLPEEYPIEKHELEGRFVAYFYDDVRMRLVE
                     QRMEIENDRYYFINYCKKRKYQVTDDFYDCILQDGEVILSKIAPSFRELVPIDFLKKC
                     HLRILP"
     gene            49035..49313
                     /locus_tag="ACG8PO_000609"
     CDS             49035..49313
                     /locus_tag="ACG8PO_000609"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0205961.1"
                     /translation="MSVEHFCDCYIALLRSQQLLTVERYGAEFGYVMQAERHRATRCY
                     EKEDQVIDGYEELCIRNNRYLQQRLYEQLMMDFSNLYRGYECSMIQER"
     gene            49333..49677
                     /locus_tag="ACG8PO_000610"
     CDS             49333..49677
                     /locus_tag="ACG8PO_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0205962.1"
                     /translation="MWRLLFLVVCGMALFYVYRVYLYPRMERWTSWYRDPLKQRKGLK
                     RYRYELLVEFSDGRQAQYYFFANYPKYNTSELLIQLFKNNPYVGLEENGREFFYRSDN
                     ISAASMNKTRIA"
     gene            49696..50607
                     /locus_tag="ACG8PO_000611"
     CDS             49696..50607
                     /locus_tag="ACG8PO_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFQ0205963.1"
                     /translation="MKKTTLAVVTTLGISLFSFQLGSSLVYADETVETPKSEIVETLP
                     GEGLPDVETELNGGTTEKPAEPEQLQSEKPEKEPVTPEPEFPESTLPTDSIEEEGVHS
                     STTTDSSIVTTPEQSGTTSSSEKNTDSSSTEQPTMSSTSEEQKEEPKQPVEKSEAIPK
                     ENKPIVPAKEVTVSVTPSGEITTNTSQGMSVPIVTSNVEELTHIPTPTTPLKVAGDQT
                     IVGVKDGIPLIQDSEGNLVKDLSIPVKKLPSGNIEIKTADGKTKVLPKTGEEIHVALS
                     VLGGVLTSIAGFVGYKRKRKGIENEGN"
     gene            50686..51678
                     /locus_tag="ACG8PO_000612"
     CDS             50686..51678
                     /locus_tag="ACG8PO_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFQ0205964.1"
                     /translation="MKKRQLRRKEKVYTEKVPKQKKIHSKKAVKWIWCLVLFLCFSGG
                     LAFFQSMKTKVQVSFLKDEIQQLQKKEVQSRSELTYTPEIESFMNRFVALYMNTFDNH
                     EQEQQRQETLKKEFYAQNFIQEPGSVSGVRKLTAASFIGLKEIETIPTASYKVTYDIR
                     SMNPESKKETSVTITQVLNLPFSISNKGCRIIAYPYFTALPKNTEKQRMITYDDTLYE
                     TVDASEAKDVEGFVEDFLDKYAKNNAKDMSYMMKEPEGLNQTAKVDQVEMKVLRYKET
                     KKPFIVKATVVFRDGDTSLLQKEQMTIVITKKTNQYYVEKLTHNWLDRKGEERK"
     gene            51675..51893
                     /locus_tag="ACG8PO_000613"
     CDS             51675..51893
                     /locus_tag="ACG8PO_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcpD family membrane protein"
                     /protein_id="HFQ0205965.1"
                     /translation="MTLKGIVDFILTQGGYFLIMVVVYLGLRNFMKSKVGNALISIAA
                     GAAVYFFLNDPTRVFDAFGKIIEKLFMD"
BASE COUNT        17708 a         8645 c        11146 g        14505 t
ORIGIN      
        1 aatgatgaaa aaagggggag aaaaatgaat tttttagttg ttttaaaaga tactctgatt
       61 gagcgatcta catttctata tgttcaattg caggaaaata aaacattgct gcatttttca
      121 ccagagttcc atctagaggg gctaacatta ggagaaatct atcaagcaga agggctgtcg
      181 gctgttttgc acttttttaa ggtagaattg gaattaccag taaaagatta tatcgagctt
      241 tctcttgaat cttgggatcg agcagtggtt gaactgtttc cagaaggttt aatgatcgat
      301 acaaacgatg ggaagatcca tctgactgca gcagaactgc aaaaacaaat gcgttatcaa
      361 atagatgacg aaaattcttt ttctattttt agacgtcagc aaaagattct aaaaagcttt
      421 ttaaaagtga tcagtaaaaa acggaatctg atcaaaacga cacagctgtt gaaaaaatat
      481 ccaggtctgc ttcacacgtc tattcaattt cctcaattga ttacgatgat ccatcgtttt
      541 atacgtatgc aacagctacc atctaagaag ctgtttcttc cattgacgga cacatttcga
      601 gtagtcaaca gagaagataa aaagaaagtg attgtgattg actttaccag aaatcggaat
      661 gtccttcacc aagttgcaat ggaaaaggcc aattaaggct ttttcttttt ggattttttt
      721 gtcataataa atattagatg atggaggaga taatatgatg caaacatggt tagaaaggct
      781 aatacgattt cagcagaact atcacaaact tatgcaggga agatatgcga aatttgatca
      841 gttgaatcgc ggtctattga tcgctgcgct tgtctttagt ttgctaagtc gttggttgcc
      901 ctatagaatc ggacaattgt tgttcatcgt ctttttcgga tggatgatat ttcgtttcct
      961 atcaaaaaaa atctacccca gactaaatga gaatcaacgt tatttgaaat atcttgatag
     1021 agtcaaagaa aaatatcatc agataaaaaa caaaaagcaa gtcaaaatgg atcgaaaaag
     1081 ttatactttt tttgagtgcc cgaattgtca tcaaaagcag cgtgcaccta aaggaaaagg
     1141 acgaatacgt gtgacatgca aaacatgtgg tataaaattc gaaacgaatg tttaagggag
     1201 aaaagaatga aattagtatt attacgtcat ggcgaaagcg aagcaaattt tgaaaattac
     1261 tggacaggat ggctagatgt cgccttaaca gaaaaaggaa aagaacaagc agcagttgcc
     1321 ggtcaaaaaa tgcttgaagc cggacttcaa ttcgatgccg tttatacctc tgttttgaaa
     1381 cgtgcgattc ttaccggaca agtcgcacta gaagaaatgg gtcagttatg gcttcctata
     1441 ataaaaacat ggcgtctaaa tgaacggcat tatggggatc ttgtgggtaa aaacaaagat
     1501 aagatgaaag aaatatacgg aaaagatcaa gtaaaaacat ggcgcagagg atatacggaa
     1561 gttccgccgt taaccgcaga aaatagattt gaccgcagat atgatcactt ggatccgcat
     1621 ttgatcccat acggagaaag tctggaaatg acagtgaatc ggatcattcc cttatggcaa
     1681 gatcatctag caccaaaact aaaagatcgg caagatttac tagttgtggg tcatggtaat
     1741 agcatccgag cactgacaaa atatttagaa gatgttcctg aagatcaaat ggatacgatc
     1801 gatatcccaa atgcacaacc aatccagtat acattagcag atgatttgac aattctggac
     1861 aaaaaaatac tttaaagaac acctttatct aaaagtgaga gatagaaaaa agaagcaaag
     1921 gaatggaaca aaaggaacat gaaaaattat atatgggact ttgatggtac attgtttgat
     1981 acgtatcccg caatggtaga tggtgcatgg caagcactaa aagattttgg tatcagtatg
     2041 gacaaaaaag aaatctactt caaaatgaaa aagtattcta ctagttattt gatcaatgaa
     2101 tcaaacttga atgccagaga gtttaatgaa ttatttcacc gatatgaaaa agaaagtacg
     2161 gaagtttccc gtccatttcc agaaacgaaa caagtactgg aaatgctaaa agacaacggc
     2221 ggtcgtcatt tcatcctgac ccaccgtttg acagaatcaa catggggcct gttaaaagaa
     2281 catcgattgg cacatctgat tgaggaagta gtagggatcg atcaggactt tcctcgtaaa
     2341 cctgatccag catctttaaa ttatttgatc gatacttttc atttagaaag aacagacacg
     2401 atgatgatcg gcgatcgtcg tttagatatc gaagcaggga aaaatgcagg agtcgctact
     2461 tgtctttacg acatcgatca ttttctgggt gagatcccgg cagattatgt ggtaggaaat
     2521 ctgaatgaaa tccttacctt gcagagcatc attaaaaagt agtatcccct ttcaaaggag
     2581 atatgttata attttcataa aggagtgata aaattatatg gaaatcagaa gacaaaaaga
     2641 agttgtagaa gcatttcatt atgatatgag attgccgaac caagaagtag aaacagattt
     2701 gaaagtaggc ttctcgcctt taaaagaaac gatcgaaaac tatccgaaag aacatagcat
     2761 cgtaggagca cgcttagagt tccgtttagt gtttgataca tatgttttat ctggaagtgt
     2821 gagtcagatc aatcatatcc tcaatcggaa aattgaaggt caaaatgatg tcacacaaga
     2881 agaagtggac gagctagtag caccgttatt cgatatcgta cgccgaatgt cttatgaggt
     2941 gacagagatc gccttagatc agcctggggt aaaattgaat tttcaatcag aaaaacaaga
     3001 atagacagta aaaacatacc tgcgatagat taaagctatc acaggtatgt ttttttatta
     3061 ctaattgtaa gcaaaaagaa tgtcatttcg ataaaaatcg tctaaaatat aaaagtcact
     3121 gaaaaaaatt ttttcaggag gatggttgaa gaatcattct cgggaacctt tacttccttc
     3181 gatggacttg tcattaataa tatgaggagg agaaaaatga caacactcat cacagtttta
     3241 gtggttctag tttttgcggc attattatat gtgttctatc gtatgcaaaa gcgccataca
     3301 aaattttcaa cacgcgtctt tacagcatta ggagttggta tcgtctttgg cggtctgctt
     3361 caattgatct ttggtgtaga cgacaaagtc gttactcaat caatggaatg gatcgggatt
     3421 gtaggtcaag gatatgtatc attcttgcaa atgctagtta tgcctttagt gtttatttcg
     3481 atcattggtg cattcacaaa aatgaaagtt tctgaaaagc tagggaaaat tagtgcgact
     3541 gtattgactt ctttacttgg aacaacagct attgcagcat tgatcggaat tggttcaaca
     3601 atgctgttcg gactccaagg agctaaattc acacaaggag ctgcagaaac agcacgaatc
     3661 tctgaactgg caacacgtca ggaaacagtt gaaaacttat cgattccaca gcagatttta
     3721 tcctttatcc ctaaaaacgt ttttgctgat ttagcaggta gtcgtgcaac aagtacgatt
     3781 ggagtcgtta ttttcgcggc atttgtcgga atcgcttatc taggcgtgaa aagaaaagcg
     3841 ccaaaagaag cagcattctt ttcagattta gtcgacagtc tgtataaaat cattatgcgg
     3901 atcgtgacgc ttgttttgcg tttgacacct tatggagtat ttgctttgat gacaaacgtt
     3961 ttggcaacaa gtgattttgc agctatcgtc aatttaggta aatttgtcgt tgcttcttac
     4021 gtagcattgc ttgctgtttt gattgtccac tcactgattt tgattggtgc aaaagtcaat
     4081 ccaatcaact atttcaaaaa agcctttccg gtattgagct ttgcgtttac ttcacgttca
     4141 agtgctggag ctttgccatt gaatatcgag actcagacaa aagctttagg agtagatgat
     4201 gcaacagcta atttctcagc aagttttggt ttgtctattg gacaaaacgg ctgtgctggc
     4261 gtttatccag ctatgttggc aacgatcgtc gcaccagcag ctggagtgaa tgtctttagt
     4321 ttggaattta ttttgatgct tgtggcaatt gtaacaatca gttcttttgg tgtagctgga
     4381 gtcggtgggg gcgcaacatt tgcttcactg atcgttttag gggcaatgaa cttgccagta
     4441 gcaatcgttg ggttagttat ctctgttgaa ccattaatcg atatggcacg tacagcagta
     4501 aacgtcagcg gtagcatggt agctggtatc gtgacaagtg cacgcatcaa agatttagat
     4561 cgcgatgtca tcaatgatga ttctaaagtg atcgatgtga atgcatagtc aaaaagaggt
     4621 tgtgatagaa gtggtcagct ttaagaaata agaagaagtt tcctaaaaat agtttctcaa
     4681 atttttgtga aatttcggct tatttccgag aagctacttc tgaaacaccg tgaataagag
     4741 gttgtgaaaa agctgccctg catcaagtaa taagaacagc caaacaaaaa tagttcctca
     4801 tattttacgt ttggttgagc ttattaccgc agatgcaagc ttttgaacac cgtttaagaa
     4861 agaggtcgtg agcctgccgt ttcactctga gtcacaaaga gcaatttcaa aaaatagctt
     4921 ctcatgtttg ttgaatattg tgtcttgtga ccgaggagtt gtcaggagaa caccgtttac
     4981 cagaggttgt gacttgtttt tagtcacgac ctcttttttt atttattcca tataaataca
     5041 cattatttat tccgaaaagt gaaagcgcaa ataacaaaaa caaccaactt atttattatt
     5101 aaagaaaagc gtatttaagt aggacgattc tgttgagagc tttcgtagat tttcccattt
     5161 tcctatggta aactgacaaa gttgagaatg ttagaacaga tcgtttagga ggaaacagat
     5221 gtttacgaat gaaataaaaa tcatgcttta tgtggacgat gtagaaaaaa atgcaacgtt
     5281 ttggagagca gttggctttg cggaaaaaga tcgtcaagaa atggatggga cgcttataat
     5341 tgaagtagga gttgaggaca gtcaaacctc atttctgtta tatgatcgag catttgttga
     5401 gtcccattca cctggcacag ctactagtgc cccatctttg atgttttaca gtaaagatat
     5461 ctttggacta tataaaaaaa tgcagacgca atcagtcaca ttaggtgata tggtacagct
     5521 tggagaagaa tatgtcttta actttgctga tcccgatggc aactactttg cggttacagg
     5581 taaataatag aaataaagac tttgtttttc tgtgcgaaac aggaaagcga agtcttttta
     5641 attcaaataa taatattatg taaacaaaaa actttccatg cgtaattata cacaagcaaa
     5701 taaatcgtca aatgcgaacg ccttctttat actgtttagg aacgaaaaga aggaaggaag
     5761 aaatacatgg attatcaagt actgctttat tacaattata caaaaatcga agaaccagag
     5821 gaatttgcca aaaatcattt agccttttgc aaatctttgg atcttaaagg tcgtattcta
     5881 gtagctgaag aaggaataaa tggtactgtg tctggaaccg ttgaaaacac gactgcctat
     5941 atgaaagcaa tgaaagctga tcctcgattt gctgatacga tgttcaagat cgatccaagc
     6001 gaaggacatg cctttaagaa gatgtttgta agataccgtc cagagcttgt aagtttaaag
     6061 ctagaagagg acattgatcc aaaccaagtg acaggtgcct atctttcacc gcaagaattt
     6121 aaagaagcta ttttagatga acagacagtg gtgattgatg cacggaatga ttatgaatat
     6181 gacttgggac attttcgagg ggctgtacgc ccagatatac gcagttttcg agagcttcca
     6241 gactggatcc gcgagcacaa agaagaattt atggaaaaac gggtagtgac ctactgtact
     6301 ggtggcattc gttgcgagaa gttttctggt tggcttgtcc gtgaaggatt caaagatgtc
     6361 ggacagctgc atggcggtat tgctacttat ggtaaggatc ctgaagtcca aggagaatta
     6421 tgggacggca agatgtatgt gtttgatgaa cggatcgctg tcgatatcaa ccaagtagat
     6481 aaacaaatcg tcggtcgcga ctggtttgac ggaacaccat gtgaacgata tatcaattgt
     6541 agcaatcctg agtgcaatcg tcagattctt gcttccattg aaaatgaaga aaaacattta
     6601 ggagcatgta gcatagagtg tgcgaaacat ccgcgcaatc gctatgttca agaccatgga
     6661 atctctccag aagaagcaga actgcggttt acagctttaa ttaaatagaa aacaatagtt
     6721 ttgattgtcg accatttcca atatgcgcta tactagcgtt gaggatggtg atccacatga
     6781 aatcattgat ttatgctatt ggggatgtcc atggcaaaca tgagcttttt gaaaaactta
     6841 ttcactatta tgatccaaag atccatcagc ttgttttatt aggggatctc aatgatcgag
     6901 gtcctcgtac aaaagaatgt tttttattag gaaagcaatt agtcgaagaa acaggagcca
     6961 tttacctccg gggaaaccat gaagaatatt ttttgcaatt tctgcaatca ccggaggact
     7021 ggtatgcgcc ttatatgaga aatggtggaa aagaaacgtt tgaaagcctg attcacccag
     7081 gggcatcgga ggaatactcc ccaactgaaa tctcgatgat gatccaatcg agatattcag
     7141 aactgattaa atttttagtc cagcgcccct tgtattatga atgggaaaat tatctttttg
     7201 tccatgccgg tgtggatttg acaaagaaag actggcgtaa gacaagtcta agagacttta
     7261 tttggataag ggaaccattt catgaaggga aaaacaatac aggtaagaca attgtatttg
     7321 gacatacgat tacaccaatg ctccatggag atatgcagac aacagacctc tggattcagg
     7381 atcataaaat cggaatggac ggtggggctg tattcggagg gtcattgcat ggggttgttt
     7441 ttgacaagga aggtattctt caagatattg aatatcagaa caacgaagga ccttggcatg
     7501 gaatctgact aatggaaaga atgaaggagc tgtgactttg ttgcggctct ttttattttt
     7561 atagagaata ttgtttcttt cagtagttta acccaggata aaaagtatga tagaataaag
     7621 aagcaatttt tagcagaaaa gaggcatttt aatgacggaa aatttgaacc agacgatcat
     7681 tcagcttgta caaaaagaac tttcagacta tcgtcctaag cagttgacta ctgtacttaa
     7741 tttgttaaat gaaggaaaca ctgttccttt tatcgcgcgt taccgaaaag aaatgactgg
     7801 aagtcttgat gaagtccaga ttcgtgaaat cgaagagcgg tatgcctatc ttgaaaactt
     7861 agaaaaacgt aaaactgaag tgattcggct aatcgatgaa caagggaaac tgactcccga
     7921 gttagaagca gaaatcaccc aagccgtgaa aatgcaacaa gtggaagatt tgtatcgtcc
     7981 atacaaacaa aaacgacgga caaaagcaac gattgctaaa gaaaaaggac ttgaaccact
     8041 cgcgaagtgg ctgatgcagc ttacagatgg agaggtgcaa tctgaagcag aaaaatatat
     8101 tgacaaagaa aaagaagtct cttctgccga agaagcgttg cacggtgctc atgagattat
     8161 tgctgaacaa gtcagcgata atgcaaaatt tcggacttgg attcggtctt atacgtataa
     8221 caaaggtatg tatgtcagca atgtcaagga tgagcaagca gatgaaaaag gcgtatatga
     8281 aatgtattat gactttgcag aacccgtcca taaaatggtt tctcatcgaa tattagctac
     8341 taatcgtgga gaaaaagaag atattctgaa agtattcttt caagtagatg aagcagcaat
     8401 tttagcttac ttggatcgcc aaatagtcaa aaatcctgcc tctccttctt cttcattcgt
     8461 cagagaagcc tatcaggata gttataaacg ctttattcag ccagcaatcg agcgagaact
     8521 gcgcaatgaa ttgactgaaa aggcagatga gcaagcaatc gctattttcg gcgaaaacct
     8581 ccgtaatctt ttgttgcaac cgccactaaa aggtaaagtt gtcctgggat ttgatccggc
     8641 ttatcgtact ggatgtaaac tagcagtcgt agatgcgaca gggaaagttt tagcaatcga
     8701 agttatttat cctcataaac ctgcagcgca agcaaaaaga gaagctgctg gaccagcttt
     8761 tatccaatta attaataaat atcaagtaga tatggtagct atcggaaatg gtacagccag
     8821 tcgggaatct gaactttttg tggctgagca gttgaaatcc gccgaccata aaacctatta
     8881 cgctatcgtc aacgaagcag gcgcttctgt ttactccgct agcgaaattg cacgaaaaga
     8941 atttccgcat ctacaagttg aagaaagaag tgctgtttct attgcccgtc gcctgcaaga
     9001 tcctttagct gaattagtca aaatcgatcc taaagctgtc ggagtaggcc agtaccaaca
     9061 tgacgtttct caaaaacgtt tggctgaaca attggatttt gttgtggaaa cagcggttaa
     9121 ccaagtaggt gtggatgtga atacagcaag cccgcagctc ttgcagcata tatcaggatt
     9181 gaataaaaca actgcccaaa acattgtcat ttatcgtgaa gaaaacggag aattcaccgc
     9241 acgaactcaa ttgaaaaaag tgccacgctt aggaccaaaa gcatatgaac aagcaatcgg
     9301 attcttgcga gtccctggtg ggaaaaacat tttggacaac acaggtatcc atcccgaaag
     9361 ttattctatt gcaaaagaaa tattaacttc agtccaactt tcagaaaaag agcttggtac
     9421 agaagaagca gtagaaaaat tgacacgatt atccgtagag aaactggctg aaagcctatc
     9481 agtcggagaa gaaacgttag ccgatatttt agctgggctg acacaacctg gacgagatat
     9541 gcgtgatgag atgccagcgc ccttattacg tacagatgta ttgagtatgg aagacttgaa
     9601 accagggatg gaattgactg gaacggtccg taatgtcatt gacttcggcg catttgttga
     9661 tatcggtgtg aagcaagacg gacttgtcca tatatcgaaa ctcagcaaaa aattcgtcaa
     9721 gcatcctaca gatgtcgtgt cagtaggaga tatcgtaaca gtttggatag aacaagtcga
     9781 tacgaaaaaa ggcagaatca gtttgacgat gctttcccct tatgaagagt aacaatgagg
     9841 agtaacaaaa attgacacaa gaagaactgc agcagttagt agaagagata tcattaaaaa
     9901 gcttccagct tcctttcaag caccaagcaa tgttcaatcc acgcttacgt acaactggcg
     9961 ggcgctatca tttaaacgat catcatttag attttaatgc gaagctattt gaagagtcag
    10021 acgaagagac gattgcagga atcatcaaac atgaactttg ccattatcat cttcatctag
    10081 ctggaagagg gtatcagcat aaagatatgg atttcaagta tcttttgaaa aaaacaggag
    10141 ggttgcgcta tgcaccagcc ttgacgaaga aacagataaa gatggagtat tatcgatgcc
    10201 aagattgttc tgccacgatc tgccgcaaga gaagaatcga tgtacgcaaa tatcgttgtg
    10261 gaagatgtca tggagaaatc aaatggattg agacaaaaag agaagacttt aatgaagcac
    10321 aaaaaatcag atgaaaggat agaaggcctt tggaaaaaag tgagcagagg aatctaaaag
    10381 actggaatca gctattaaat tttttagccg cacctgaaga gggaaatttt gaggaactga
    10441 cggcacctac acttatttta gctggaaact gtcttcctat cttagctgat aaagcagcac
    10501 aaatgtattt aaatggccaa gtagatcaat tgtttttggt cggaggggtg gggcatgcga
    10561 ctagaatcct ttatgagaat tttgaaaagc aaggatttca ttttgaagat ggcatgagtg
    10621 aaagtgaaat ctgtcgtcag tatctaaaag aagtctatga tttgcccgat aaggcttttc
    10681 tgatagaatc gaagtctaca aattctggag agaacgccac tttttcactg gagattcttc
    10741 attccttagg agctgtacca gaaaaaatct tattgatgaa tgatccgaca ctgcaacgca
    10801 ggactcgagc aacttttgag aaagtatggc aaaatgaaca aactattttc gcgaatgctg
    10861 tgccatttgt gccggaaatc cttcactttt ctgaagagat catctttaca gcaaaagaac
    10921 tgaatcatca atggcctaag gaatatttcc atgcgctagt attaggagaa atggaacgtc
    10981 tccatgacga tgagaacggc tatggtccga aaggaaaaga ttttattcct cacattgata
    11041 tatcagagga agtttggtct tcttacacca gaattaaaga gtcaataaaa acggattttt
    11101 ctcgtacttg accattttag caagcgaatc ctcctttcca aataaatttg gttgaaattt
    11161 tcaatcgttt ataagataat gaggagcgtg ttgaatgtaa tagctaaaag aatggacgct
    11221 agataagaga agaggattca aatggaaaat caaaataaat tgtatcattt tgttggtatc
    11281 aaaggttcag gaatgagctc cttggctctt gttttacatg aacaaggtct aaatgtccaa
    11341 ggatcagaca ttgaaaaata tttctttaca caaagagatt tagaaaaagc aaatatcacg
    11401 attctgccat ttaacgcaga taatgtaaaa ccagggatga cgatcattgc aggaaatgca
    11461 tttcctgatt cacatgaaga aatccagcga gcaaaagaat taggtttgga agtgattcgc
    11521 tatcacgact ttattggtca ttttatccaa aattatacga gtatcgctgt aacaggctct
    11581 cacggaaaaa caagtacgac tggactgctc tctcatgtat tgagtggtgt ccgtccaaca
    11641 agttatctaa ttggagatgg aacaggacat ggtgatccgc aagcggaatt cttttcattt
    11701 gaggcctgtg aatatcgccg tcatttcctt gcctattcac cagactatgc aatcatgacc
    11761 aatatcgatt ttgatcatcc ggattattat acaagtatcg acgatgtatt tacagctttc
    11821 caaacaatgg ctggacaagt gaaaaaagct atttttgcgt atggtgacga tgcttattta
    11881 cgcaaattaa aagcaaatgt gccgatttat tattatggtg tcacagaaaa cgatgatatc
    11941 caagcacgaa acatcgaacg cacaacttct ggttctgctt ttgatgtcta tcatggggat
    12001 gaatttgttg ggcacttcac tgtaccagca ttcggtaaac acaatatctt aaatgcttta
    12061 ggtgttattg cagtagctta tttcgaaaaa ctggatctga aagaagttgc agaagaaatg
    12121 ctgactttcc caggagtaaa acgtcgattc agtgaaaaga tcgtagcaga catgaccgta
    12181 gtcgatgatt atgcgcatca ccctgcagag atcaaagcaa cgattgatgg cgcacgccaa
    12241 aaatatccag ataaagaaat aattgcagtt ttccaacccc atacgtttac gcgtacgatt
    12301 gcattgatgg atgaatttgc agaagcactt gatttagctg ataaagttta cttatgcgat
    12361 atttttggtt cggctcggga agaacaagga aacgttaaga ttgaagacct tggagcgaaa
    12421 atcaaaaaag gtggagaagt catcaaagaa aataatgtat ctccattgtt agattatcat
    12481 gatgcagtag taatcttcat gggtgctgga gatgttcaaa aatttgaaca agcgtatgaa
    12541 aaacttttaa gcagcacgac gaaaaatgta ctgtaataca attactgcag tgtccttata
    12601 aaaatagaaa gagggggagt ggcaaaaatc ttgccaagcc cccttttttg caacttatga
    12661 taaacggtgt tcaaaagctt gcatctgcgg taataagccc aatcaaatca aaaacatgag
    12721 aagctgtttt ttcttggctg ttcttattat ttgatgcagg acagcttttt cacgacctct
    12781 gtaaactata ggaatctgtt aaatcaaggg agcagaactt gccttttacg ctacttttgg
    12841 tacaataatg tatgattaaa aataaactgg agggtttcta tcttgaacat aggtaagccg
    12901 agaaaactag gaaaaacaat aaatgaaatc gaggaaggcg actcgttatc cttaacagaa
    12961 tctattgaag ataaccagct tttgctgtat ttgggattga cgaatgatgc aaatcctttg
    13021 tatatccaac atgactttgc acaaaaaaca gaatatcaac agccaatcgt tccatcagtg
    13081 atgctgatgg ggatcatcac aagtgcaatt tcaaagcatc ttcctggacc aggatcacat
    13141 gttgtcaatt tttctgtcaa ttttattgaa ccggttttcc attatgaaac actgactttt
    13201 cagtttgagg tcataaaagt agataagatg aaggatgtgg tgacgatttc tgtagaagcg
    13261 gagaatacga acgaagatcg tgtgttggat gctgtcgtta tggtccaacc gccacaagtt
    13321 cctgaattgg aaatagcaat cgaaagtgaa ggtgaattga atgaataatc caagtacatc
    13381 aatgtcaggt atcaatcgtt tttatgcaaa agtttatgga tttttaggta tgggacttgc
    13441 ccttagtgca gtcacttctt acttggctct ttgggtattt cctttgcaat tagcaacttt
    13501 tatcaataat tttccactag gctttgtggg tctgtggatc gttgaactga ttttagtaat
    13561 ggttctaggc gtaaaagcac aaaagaatcc gagcttagca atcggtggat ttattgccta
    13621 ctctcttttg aatggcataa cattagcggt tactcttgca tactacgata ttggaacagt
    13681 aacgcaagct ttcttgacag ctactttgat gttcatgggg atgtctgttg tcggagtaac
    13741 aacaaaaaga gatcttagcg gggttggaag agcaggacag gcagctttaa tcggagtgat
    13801 cattgcacta gtcttgaaca tcttcatttt gagaagtagc gtagttgagc tgttcatctc
    13861 cctactgtta gtcgtcatct ttgcagggat tacagcttat gataaccaaa gcatccgtaa
    13921 tatctatgtc cagacaaacg gaacagcaca tagtggaatt gctgtattca tggcattgaa
    13981 gctgtatttg gactttatca acttgttctt ggcgtttctg agaatctttg gacggaacaa
    14041 ctaaaaaaga aaaaaattca tcagttgggg ctataccgaa ttatttggta tggtcccgct
    14101 ttttcttttc gcattccgca ggattctttg ttagaatgaa taagaacttt acagtaagga
    14161 gttaagaaga tgacaaaaaa taaattatta ctcgttgatg ggaacagtgt ggcttttcgc
    14221 gcattctttg cgcttcacaa ttctcttgaa cgtttcaaaa atagaaacgg tcttcataca
    14281 aatgcaatct atgcattcaa tacgatgttt gaaaatgtta tgcaaaaaga gcaaccgact
    14341 catgttctgg ttgcgttcga tgcaggaaaa acgacttttc ggacagaaat gtatgcggag
    14401 tacaaaggtg gaagatccaa aacacctggc gaattcaaag aacaaatgcc atatatacgt
    14461 gatttgttga caggactagg ggtacagtat tacgaattgc ccaattatga agcagatgat
    14521 attatcggta ctttggcaga aaaagtagac aaagatcaat ttgatgtcgt cgtgctttca
    14581 ggtgatcggg atttgactca attagctagt aaagaagtaa aagtggatat cactgtaaaa
    14641 ggtgtcagcg atatcgaaag ctatacacca gaacatgtag cggaaaaata tgatgggttg
    14701 acgccaaaac agattattga catgaaggga ctagccggag atgcctctga caatatcccc
    14761 ggtgtgacta aaatcggtga aaaaacagct atcaagctat tgaaacaata tggctcagtc
    14821 gaaggaattt atgaacatat cgatgaaatg aagcaaagca aaatgaaaga aaatctgata
    14881 aatgacaaag agcaggcttt cttatcgaaa aaattggcga ccatcaatac ttcagcgcct
    14941 gtggatgtct ctatcgactc attgaagtat gaagggaaaa atctcgataa actcgttccg
    15001 ttttataaag aaatggattt caatcagttc ctttctaaat tgaatatcgt agaagaagaa
    15061 gtaaaaatgg atgatatttt gtttgaagtc gttcatgaat tcaaagagga aatgtttact
    15121 acggatatgg cgttgtacgt ggaaatgatg ggcgacaatt atcatacaga ggaaatcgtc
    15181 ggagtagcgt gggggacaga aaagaaaatt tatgtcacga acgatctatc tgtattcgaa
    15241 aaccgagcgt tccattcttg gatcactgat ccgactcggc tgaaaaaagt atatgatgcg
    15301 aaacgaactt atgttgcttt aaaccgctat actggaaaaa cgaaaggaat cgattttgat
    15361 gtcttgctag gtgcatacct tctcgatacg aatgaaaaaa gcacagatat cgaaggaata
    15421 gctgctcatt acggatacaa cgatatccaa tcagatgaag ctgtatacgg caaaggggcg
    15481 aaaaaaggtt taccagaaga agaggaactc ttttttgctc atttagcgcg aaaagtagct
    15541 gcaatcaatg cgttgacacc aaaattgagc caagaactgg cagaaaaaaa ccaagaagat
    15601 cttttccgga aaatggaact gccattgtct atcattttgg cagagatgga aataagcggg
    15661 atcaaagtag atgcgactcg cttacaagaa atgaagggcg agttctctgc tcgcttaaga
    15721 gagatcgaac aaaaaatcta cgaagaagcc ggcgaagaat tcaatttgaa ttcaccaaag
    15781 caattaggag tgatcttatt tgaaaaaatg ggattacccg ttatcaaaaa aacaaaaact
    15841 ggatattcca cagccgtaga tgtactggaa cagcttcgtg aacaagcacc aatcgtagag
    15901 gatatcctca cctatcgtca aattgcaaaa attcaatcta cctatgtcga gggactgctg
    15961 aaagtgatcc aatcagatgg aaaagtgcat acccgttatg tccagacatt gacccaaaca
    16021 ggacgattaa gctctgttga tcctaatttg cagaacatcc cgattcgatt agatgaaggg
    16081 agaaagatac gtcaggcatt tgttcctaga gaaaaagact ggctgattta ttcttctgac
    16141 tattcacaaa tcgagttacg cgtacttgcg catatctcaa atgatgaaca tttgaaagct
    16201 gccttcctag aaggtcaaga tatccatgcc agcacagcga tgcgtgtatt tggaatcgaa
    16261 aaggcagagg atgtcacacc aaatatgcgt aggcaggcaa aagcagtgaa ttttggtatc
    16321 gtgtatggca tctctgatta cggactttca caaaatctag gaatcagccg aaaagcagca
    16381 cagcaataca tcgatacgta ctttgaaaaa tacccaggtg tcaaagaata tatggaaaga
    16441 atcgtacgcg aagcaaaaga ccaaggctac gtcgaaacgc tgtatcaccg ccgtcgatat
    16501 ctgccagaca tcaattcaag aaattacaat atccgttctt ttgcggaacg aacagctatc
    16561 aatacaccaa tccaaggaag tgctgctgat attttgaaaa tcgcgatgat cgaattggat
    16621 aaacgattaa aagaaacagg tcttcaagct acgatgctgt tacaagttca tgatgaactt
    16681 gtcttcgagg taccggaaaa agaacttgaa tcattagata aattggtgaa ggaagttatg
    16741 gaacaagctg tttctcttca tgtaccactg atcactgaca gcagttgggg aaaaacgtgg
    16801 tacgaagcaa aataaacatt gtttaagaca gagcaatata atgaaggtga aaagaaaaaa
    16861 tgccagaatt accagaagta gaaaccgttc gtaaaggatt ggaacggtta gtagtaggaa
    16921 aaacgattca aaaagttcag gtcttgtggc ccaagatcat cgagcaacca gagacaccca
    16981 tctttgaggc ttctcttgtt ggcgaaacca tccaatccat tggacgtaga gggaagtttt
    17041 taatttttca tctcgatcat tgtgaactga tctcacactt gcgaatggaa ggaaaatatc
    17101 aatttacgaa agaaaataca ccaatcgata aacatacgca tgtacttttc ttttttgagg
    17161 atggaagcca acttcgctac aacgatgtac gaaaatttgg tcgtatgaca atcgtcgaaa
    17221 aaggagcaag tgcaacttac cgaggaatca tgaaattggg acctgagcct ttgccagata
    17281 gcttcttatt agcagatttc gcaaatggac tgaaaaagtc ccacaaagca atcaagccat
    17341 tgctgctaga ccaacgtcta gtgactggtc taggtaatat ttatgtagat gaagcattat
    17401 gggaagcgaa gatccatcca gaacaaccgg cgaacacact gcgccccaaa gaggtagaac
    17461 aattacgtct atcgatcatt gatgttttgg atcgtgcaat agaagccgga ggtaccacga
    17521 ttcgcagtta tttgaatgca ttaggagaat caggcggatt tcaagtagca ctgcatgtct
    17581 atcagcaaac tggcaaacca tgtatacgtt gtggaacgcc gatcgtgaaa acaaaagtgg
    17641 cacaaagggg gacgcactat tgtccgaatt gtcaaaggct aaaaatcgca aagtaggatt
    17701 tgttttaggt ttgacgggtg gaatagctac cggtaaaagt acggcagcca aagtctttca
    17761 gtctcatgga tttcctttga tcgatggcga tgtgattgcc agagaaactg ttgagcctgg
    17821 aacgcttgct ctgaggaaaa ttgtatcagt gtttggccaa gaggtattgc agcctgatgg
    17881 gcaactagat cgagggaaac ttgggatgat tgtttttcca tcaaaggaaa aacggcaaaa
    17941 acttgatcag ctattagatc cgtttatccg aaaagcgata aaagatcaaa tcgcctctct
    18001 aagttccaat catcctttag taattgtaga tatcccattg ctctacgaag gtcattatga
    18061 ccactatatg gatgcagtag ctgtcgttta tactacgcca gaaactcagc ttcagcgttt
    18121 gatgaaacgt aatcaactga cgctagaaca agcgcagcag agaattagta gtcaacttcc
    18181 aatcgaagaa aaaaagcagc gtgcggatat tttgttcgac aacaatggaa caaaagaaaa
    18241 acttgtggga caaatcgaaa attggctttt tgctaatcgc tttctttgag tatctgtgtc
    18301 aattttgcta tcctgagtat atagaaagtg agggatagcg atgggatcat taaaaaatca
    18361 aaaggataaa gtcgtaggta acgcaaaaga aaaaatcggc gagatgctca ataaagaaaa
    18421 tctaatcgat gaaggtcgta cgcaatcaga aacagcagaa gaaaaagaaa agcagtacca
    18481 agcaagaatc gatgaaatag aagaccagca ggcagaagaa gaagcgaagc ggcaagattt
    18541 gactcaagaa cgaaaaaatc cgacacctga tcgtagtaaa acaccgctcc cagaaagtga
    18601 ccacattccc gatgagcaaa aaatcgagga acagcgaatg aaacactata caggaatcga
    18661 agagaagtta taaaatagta aaaaatcgag atgtgacttg aagcagatga gaaggtgttc
    18721 atctgcttca agttttttgt gctagatgac taaatcagct tttgtgtaag ttatttctca
    18781 gtaatcaagg tgatcagtat tggaaaattg aaatttagta aggacagggg acacaattac
    18841 gcatgaaagt agaaaaaatt gttcttatat ccgtgttttt tgggtaggac agtcgcgttt
    18901 gtgatttgtt tcgtgttata atacaactag ttgtaaagaa agacacatcc tttatataaa
    18961 atgcgatgca agaggtgaga caaaatgaga tgtcccagat gtcaacataa taattcacga
    19021 gtaatcgaca gccgtcaagc agatgatgga cgtgcgatcc gtcgccgtcg cgaatgtgag
    19081 aattgttcgt tccgattcac tacatttgaa cgaattgaag ctgctccgtt attagtaatc
    19141 aagaaaaacg gggaacgcga agagttcaat cgcgataaaa ttttaagagg actcatccgt
    19201 tcagcagaaa aaagacctgt agctatggaa caaatggaac aaatcgtcaa ccatgtcgaa
    19261 aaccgcgtac gcagtctcgg agaaaacgaa gtatcgacga atctaattgg tgaatatgtg
    19321 atggaagatt tagttgattt agacgaaatc gcttatattc gttttgcaag tgtttatcgc
    19381 cagttcaaag atatgtctgt tttcttaaaa gagttgcaag atatcgtgga taaagcaaaa
    19441 gaaaataata aagagaataa taaagaaaac aattaagaaa caaaaaggag gcatttcctt
    19501 ggaaaatgct tggaaagagc ttcaaccaaa aaatatttac caaggaagaa aagcggctcc
    19561 gctgagagaa gacagcaaga acgccttgct gtatctttat cagccactaa tcggcggaga
    19621 agccttgagt atctatttca ccctgttatc agaaatctct caagaatctg gcaaaggtcc
    19681 tgaagggatg catgcagatc tgcttagtag tctaggctgc ggtatcccgc aattttatga
    19741 tgcaagaaaa aagctagaag gaatcggctt actggatgtt tattttaaag aagatagtga
    19801 tctgggacct cgttttattt atgagctgtt agaaccactc attcctaaag catttttcaa
    19861 agactctgtt ctggcttttc ttcttctgga aaaagttggt gaacgacgat tccagaagct
    19921 tacccaattc tttgaaccta aaaggcatac agcagaggga tatcaaaaaa tcacaaaaaa
    19981 atttttagat gtttatcgct tcaatgaagc agtgtacgct gctaatcaag aaaacatcga
    20041 atcgctgaat aaatcttttg aagaatctga atcagtagca ttacttgaag cagattcgac
    20101 attcgattgg cagtttttga atgattggct ggcaaaaaaa catattgcaa gtcttgatga
    20161 tccaatcatc aaacaagtcg acatgtatca tcagctttac ggtttcgatg aattgacact
    20221 tggcgaactg atcagtcaat cttatgactt tagtgaacaa aaagtatctg aaaaagaact
    20281 gcaaaaagcc attttggcaa tgcagagacg tccggtgaag caaacaaaag aaacagttgc
    20341 ggtttctgaa aacagtaatg aaccctcgtc aaacgaacat ttgacaaacg cagcgcttca
    20401 gctgataaaa gaagcacaga gcattccacc gatgaaatat ctggaagcaa tcaagtcaga
    20461 aaaaggcgga tatgtttcta aacaagaaaa ctggttactg caagatcttg tttctcaatc
    20521 aggtctttcc agtagcgtga tcaatgtttt gctgaattat gtactagtga tcaagaataa
    20581 tgctagctta aatgccagtt atgtaaatac agtggcaaat gaatgggcac aaaagaaaat
    20641 cgccacagca gaagaagcga tccagcacat tcggcaaata agtaatcaag caaaacaatc
    20701 aaaacaaaag aagcaaacaa attatcaaac tggcaagcga aatgttcgac gggaaaagct
    20761 tcctgattgg gtcaaccagc caaaagatga aaaacagatc agcagcgaga aaaaagcaga
    20821 gattgaccgc cgttttaaag aatacctggc aaaaaaggag ggtgatatct agtggaagac
    20881 gtaggcaaag aattgaagag gatcatcagc aaacgcaatt atcaagaaaa gtatgctgaa
    20941 atgatcgctg aagtcttaaa tgatgcggat gttcaagctt ttctaagcgc acaccaagaa
    21001 gaattgacac aagcggatat tgaaaaaagt tacgctaaat tgtatgaatt cgtgcaagaa
    21061 aagcgtaaat atcaaaaaaa cgatccaacg atgatcgctc ccggctatga gccgaagctg
    21121 acgttgaatt ttcattatgt ggatgtcact tatgtaccaa cggaagcatt gttagcacgg
    21181 caaagacaag aagaaatcag aagccgagta aaagcaatgg atatgccaaa agatattcaa
    21241 tcggcttctt ttgaaaactt tgaacgtaca gatggacgag gatatgcgag cttagaagcc
    21301 ctagattttg tagagaaata tactgctgat ccaaaagggt atcataaagg actgtatcta
    21361 gtaggaagtt ttggtattgg aaaaacatat ttattaggag caacagcgag agaattagcg
    21421 attgctggat tcaccacgac gctagtccat tttccgacat ttgccgttga gatgaaacag
    21481 gcaatcggaa aagatcaagt ggcagaaaag ttagatgcag tgaaacgttc acctattttg
    21541 atgatcgatg acattggtgc agatgcgatg agtagttgga tacgagacga agtattcggt
    21601 gttatccttc aatatcggat gcaggaacag ttaccaacct tcttctcatc gaattttaca
    21661 atggatgaat tggaacagca tttgtccgtc actcaacgtg gagatgaaga gccgctaaaa
    21721 gcgaaacgga taatggaacg catacgttat ttgacaaaag aaatcgaaat gactggtcgc
    21781 aaccgacgca atggttgaac agttgacaag tccaactgac atttgaccta gtagtaaaag
    21841 caggtcagcg aatcattata tggacagaaa tgaccggaga caatcaccgc aacaagtaat
    21901 gcagaaactt aaacggagga ttttttatga gggctatttt ttctagtata tctgtagttg
    21961 gtctattttt tcttttagca ggctgcggta gcgcagatag caacacaggt tctgttgatt
    22021 cgattgatgc gggtcaaaca gtaaaaacaa gcagtacgaa aacggagaga caaagcatat
    22081 ttgtcggaac gattgaaagt atcactcaag tagaagcaga agaagaaacg cttcagttga
    22141 aactcgtcga tgtcatggcc aaagaagatc cagcgaatat gggaacgtca ttcagcaatg
    22201 acggagtgat cttaaatgct actgctgaaa aaataagcgt ggacataaat gaattgaaaa
    22261 tcggtgacaa agtccaattt acattagttg agatgccgat catgacgatg tctatcccac
    22321 cgcaagttcc tggagattcg attattgatg tttcattgaa caaataacca ataacatttt
    22381 ttcaaaaata agaaaatgtt gttttataga acgaaaaggt atatttttta aggagatatg
    22441 ggaatttttt tattcctagt ctccttattt tatttggaaa atgctataat tatctagaag
    22501 gagtgaagct atgaaccatt tagatttatc tcagcgctta gccgcagtgg gtgactttgt
    22561 ccctgaatcc gctcgtttag cagacattgg atcagatcac gcttacttac ccgttgcatt
    22621 gatattaaga ggaaaaatcg aatatgcagt tgctggtgaa gtcgttcaag gtccttatga
    22681 atcagcacgt aagcaagtca aaaaagatgg attagaagac cgtatcaccg tacgccttgc
    22741 aaatggtcta gatgctatcc agccacaaga tggtattaca gctgtcacga ttgcgggtat
    22801 gggaggttca ctgatccgag atatcctaga agctggcaag ttgaatcaac gcttaagcgg
    22861 acaagaacgt ttgatccttc agccaaacat cggagagcgt acattacgat cttggctgca
    22921 agaaaatcag tataaaatcc aatcagaaaa aatacttgaa gaaaataaaa aaatatatga
    22981 aatcattgta gccgaaaaag aacaaaaacc agttacttac aatgaaatgg agctgatgtt
    23041 cgggccattt ctgcttgaag agaaaagtga gatcttcaaa tcaaaatgga caagagagct
    23101 tcaacaaaga aaagctatcc gtgaacagct caaacaagca gctaatcaag aggcacgtca
    23161 aaaacaaatc gagcaggaaa tccaaatgat tgaggaggtt ttaaagccat gacaatcagc
    23221 ggacgcactt tcatccaaaa gtttgaaagc tattgtcctc agtggctagc tgaagaagga
    23281 gacccagtcg gactgcatat cggaacattg gataagccaa tccaacgagt aatgatgaca
    23341 ttagacgttc gtccagaagt ggtcgaagaa gcaatcaaga aaaaaatcga tttgctgatt
    23401 gctaaacatc ctccaatctt tcgtcctgta aaacggctgg ttactgatca gccacaagaa
    23461 aaaatgtatg cagatttgtt gaaacatgat attgccgtct atgcagcaca cacgaacatg
    23521 gatattattt gggatggact gaatgactgg ttttgtgaac ttctcggtat tgaagtggag
    23581 agttatttag taaagactca tgagattcat tataaaaaac tggccgttta tgtacctgtg
    23641 gatcatgcac agaaaatgcg tgaagtatta gctgcagctg gtgctggtac acaaggtgat
    23701 tataccggta caagttttac ctcgattgga cacggtcgat ttactccgga agctggtgca
    23761 caaccagcga ttgggaaagt agggaagaca gaacaagttc aagaggcaaa agttgaggtt
    23821 attttgccgg aaactatcga aaaacaagtg attcaagcca tgcggtcagc tcatccttat
    23881 gaggaacctg cttatgatct tttcgctata gatgaaccag tcgaaatgtt tgggttagga
    23941 cgagtaggtg aattgccgca agaaatttct atagaggcat ttgtagaaca agtaaaagaa
    24001 gcgttccaac tagatggact gagaatagtc cagccaaaaa atgcaaaatc ctctgtgaag
    24061 cgcattgcga tttgcggtgg aagcggtgaa aaattttatc cgcaagcaat cgcccaaaga
    24121 gcagatgtct atatcacggg agatatctat tatcatacgg ctcatgatat gcaaagcgct
    24181 gggttgattg caattgatcc cggtcattat attgaatcgt tatgcaagca acgatttatc
    24241 gagaaatttg aatcttggaa acaagaagaa aattgggata ttaacttttt cgtttctgaa
    24301 acaaatacaa acccatttca atttaattaa aaggagagaa tactatgtac gaaaatcttt
    24361 tgccacgttt tttacgatat gtcaaaacag aaacacgctc cgatgcaaca agcaaaacga
    24421 ctccatcgac tcagacacaa gttgcttttg cgcaagaatt gaaaaaagaa ttagaagagt
    24481 taggcttagc tgatgttttc tataatgaag ataacggatt cgttatggga acattaccaa
    24541 gcaatgttga tcatgaagtg cgttccattg gatttatttc ccacatggat acagcagatt
    24601 ttaatgcagt aggagtcaat ccacaaatca ttgaaaacta tgatggcgag tctacgatcg
    24661 tactagatgc ggaagaacgc tatacattga atacaaaaga ttttccaaat ttgaaaaatt
    24721 atgaaggcca cacattgatc acaacagatg gcagtacatt actgggagca gatgacaaat
    24781 cagggattgc ggaaatcatg actgcaatgg agatcttgat caataatcct tctatccctc
    24841 acggtaccat caaagtggct tttggtcctg atgaagaaat cggtgtcgga gcggataaat
    24901 tcgatgttga gcaattcgat gtagattttg cttatacagt cgatggtggt cctttaggtg
    24961 agttgcagta tgaaacgttt agtgctgctc aagcaaatat tacgatccaa gggaaaaatg
    25021 tacatccagg gacggcaaaa gatacaatga tcaatgctct acagttagca atcgattttc
    25081 ataatcagct tccagccgat gaagttccag aaaaaacgga aggatacgaa ggtttcttcc
    25141 atttgatggc attggatggg aatcctgaag aagcttctat gagttatatc attcgcgatc
    25201 atgatcgtga aaaattcgaa gcacgcaaag cacaaatcac acatatccaa gaagagttga
    25261 atcagcgatt tgatcaagaa cgaatcaaag tagatatgta tgatcaatat tacaacatga
    25321 aagaaatcat tgaaaaagat atgagcatcg tggaattagc gaaacaagca atgatcgagc
    25381 ttggtatctc tccagtgatc gaaccagttc gtggaggaac agatggttca aaaatctctt
    25441 acatgggtat tccgacgcca aatctatttg ccggaggaga aaatatgcac ggacgattcg
    25501 aatttgtttc tttacaagta atggaaaaag caacagacgt gattgtaaaa attgcagaat
    25561 tgaacacaaa gagttaaaat cgaaaaagaa cagcttatga caaggcgttc gtcccaatga
    25621 tggaatgtca tctgtctaag gctaaagggc aggacaatgg aattggttgt cccgccctct
    25681 tttttgaaga gtaaatagga ggtaaagaat ggaaaagctg ctagtttttt ataacaaaag
    25741 ttcagggaaa gatgagggtg agaaattagc acgctggttt caagattatg taaaagaaaa
    25801 gcagccagaa ttatatgtag aactagctga aactggccca tcgattgatg atgcggaatt
    25861 agtcaagaaa gcagaagcca tagaagctga tactttagta attataggtg gagacggcac
    25921 gattcatcat atcgtccaag cgttccaaaa aacattatct caataccatg tcggattact
    25981 tccaggtgga actgtcaaca accttgcacg tgttcttgag attcccctca aaaaagaaga
    26041 agctgctgac gtgatcctgg aaggcaatgt acggaaaata gactatggtc aagtcaatga
    26101 tgaagtcatt atctctacac tgacaatcgg aatcttagca gatacagcag tttggatcag
    26161 tcaagaagaa aaacagcgtt acggctcgtg gatctttatc aagaagtttt ttaagttatt
    26221 gctgaagaag aaaaaatatc atttggattt agaaacagac gaggagcatt ggaaaggaaa
    26281 atcccaactg ttgacagtca cgatgtctaa ctccgtgggc ggttttacaa attttgacga
    26341 ctcagcgacc ctagatgatg gaaaatttca tgtaacgatc gtaccttcct tagatatttt
    26401 ccgatttatt ttttatttgc ctagaatcat gagaggcaaa ttctatacga tccccggcat
    26461 tacttatttc acagccaaac gcatgaaaat ccaagcgcag gaaaaatctg tgggcactcg
    26521 aacggatgga gacacgacag atgatcttcc agttgaatta tctgttgtat caaaaggatt
    26581 aacagtttat tcaccaaaga aagaagaaaa agcttagaaa tagtagttaa cagaaagttg
    26641 gaagaagagt aatggatgta ttgttacaca atcctttgtg gttttacctt ttcattgtga
    26701 atttttactt attttgtctg atggggtatg ataaacatca ggctagaact agcggctggc
    26761 gtgttcctga gaataatttg atatttgtca gcctgttagg cggtggtttg ggaggattga
    26821 tctctcgaaa agttttccat cataaaactc ggaaaaagaa gtttactata ggattcgctg
    26881 caggtgtact tgttgatttg attctcattt attttttcca ttaaggagca ggagatgtcg
    26941 tctaaaggaa cgaaaactaa aatcattctg aatctgcttt tgctgattgt gatttttgcc
    27001 attatttatt atttaatcaa tcaatcattt gcagatatct ttgccgaatt gctgtctacc
    27061 agtgttgttg ttttggcagc ggctgttata ttagggacag tttaccaaat cgctgaaggg
    27121 cgctcaatca aagaaattgc tgaacctttc gcgccgaatt ttacaacgaa agatgggttc
    27181 ttgacctcta gctatattgc gttttaccga atcgtttcgt ttggtacagg aacgctgatc
    27241 tcggagattt atttttataa caaaaaagga atgaagctat cccagggagc aggggtgaca
    27301 gcactccata tgattatgta caaactggca gtgatcactt atgcattagc tggcttggtc
    27361 atccaattct ctctttttta ttctaatggt ccatcaatga tctggtttat catcgctggt
    27421 atccttttga cgatcgtgat tgttgccttt ttattggcga tgtccgtcag cttgaatgtt
    27481 caaatatttt ttgtcagctt atcgaataag ttatttaaaa gtgagcgttt gcggaaatgg
    27541 gtggatacat gcaatcagca gatctactct ttaagggaaa cggtccaaac cattatccaa
    27601 gacaggactg cgctattgcg gatttatcta tggaatatgt taaaacttgt attttggtat
    27661 gtacttcctt atttgattct gattgaaaac catcctaaaa tagattttct actgaccatt
    27721 tcgtttatca gctttgcagt agtgctttca ggggttattc ctacgcctgc tggtattggt
    27781 tcctttgaat ttgtctattt gttattattc agaccagttg ttggaacagt tgacgcagtt
    27841 tcttcgcttt tactgtatcg tttcagttcc tttatcttgc catttctcta tggatttgtg
    27901 tatgtgattg ctgaacggcg aagagtgatc aaagaagaaa taatagaagt aaaaaaagag
    27961 aataaagaaa atcaagggta ggtcttttat aagatctacc ctttttatca aatcaaagaa
    28021 aaacgttatg gataagataa ttttacgttt gtttggctct ttttttatat acatacaatc
    28081 aattttgttt ttttaagatt tatataagtc gagaaaaaaa gtcaaaaaac tatgtgtata
    28141 ttttgaccaa aattgaccaa agcgatataa tagtttttgt aaagatcaaa gaaataagtg
    28201 atcttacctt taattaggag ggtatacctg tatgaatatt gagaaaatga ctacgacgct
    28261 tcaagaagcg attgctgaag ctcaacagat tgcggtcact cgaaaacatc aagatatcga
    28321 cattgcccat gtatggaaaa tctttttaca atctaatcat tttggtcgaa atttctatac
    28381 ggatgccgga ttggatgtcg aatcttttga acatgaaatt gatcgtctac tggatgagta
    28441 tccagttgtt tctggtggaa acgtccaata tggacaaaac ctcagccaga atttctttcg
    28501 attattgaat gaagcagacc aaatacgtga aagcttcggc gacgagtttc tttcaacgga
    28561 agtcgttatt ttagcgttga tgaaattaaa aaattatccg ttaactgttt atttgaataa
    28621 aaacggatta tctgaaaaag aattgcgtaa aaacatcgaa gagatgagag gaggagaaag
    28681 agtgacctca caaaaccaag aagaacaata caaagcatta gaaaaatacg gcgttgattt
    28741 agtacagcaa gtcaaaagcg gaaaaatgga tccaatcatc ggtcgtgatg aagaaatccg
    28801 tgatgtcatc cgtattcttt cacgaaaaac aaaaaataat cctgttctga taggagaacc
    28861 cggtgtaggg aaaacggcaa tcgttgaagg gcttgctcaa cgtatcgtac gcaaagatgt
    28921 gcctgaaaat ttaaaagata aaacgatttt ttcattagat atgggtgcat tgatcgctgg
    28981 agcaaaattc cgtggtgaat ttgaagaaag actaaaagca gtcttgaaag aagtcaaaaa
    29041 aagtgatgga cgtatcctct tgttcatcga tgaaatccac aatatcgtag gggcaggaaa
    29101 aacagaagga agtatggatg ccggaaatct gttgaaacca atgcttgctc gcggtgaatt
    29161 acatttgatc ggtgccacaa ctttagatga gtaccgtcaa tatatggaaa aagacaaagc
    29221 gttggaacgt cgctttcaaa aggtattagt caaagaacca acagtggaag atacgatcag
    29281 tattctgcgt ggattaaaag aacgttttga gatccaccat ggtgttaaca tccatgacaa
    29341 cgcattagta gcagcagcta ctttatctga ccgttatatt actgatcggt tcttaccgga
    29401 caaagcaatc gatttgatcg atgaagccag tgcaacgatt cgggtagaaa tgaactctat
    29461 gccaacggaa ttagatcaag ttactagacg tttgatgcag ttggaaatcg aagaagctgc
    29521 actgaaaaaa gaatcagatg atgcaagtaa aaaacgcttg aaaaatttgc aagaagaatt
    29581 agctgaactt cgtgaagaag caaatgcgat gaagatgcag tgggaaacag aaaaagaaga
    29641 agtcaattct gtttcagcaa aacgtgctga aatcgataaa gcaaaacacg aattagaaga
    29701 tgcagaaaat aactatgatt tagaacgcgc tgctgtattg cgtcatggta cgatacctca
    29761 attagaaaaa gaattgaagg aattagaagc taaagcaaaa gacagcgaga ttaagatggt
    29821 tcaagagtcc gttaccgaaa atgagattgc tcaagtcgtt ggcagattaa cgggtatccc
    29881 cgtaacgaaa cttgtagaag gtgaacgaga aaaactaatt aaattgaatg aaacacttca
    29941 taaacgagtg attggtcaag atgaagcagt tgatgcggtc agtgacgcgg taattcgttc
    30001 acgggctgga ttgcaagacc caaatcgtcc tctagattct ttccttttcc taggaccaac
    30061 tggtgtcgga aaaaccgaat tagcgaaagc gttagctgag aacttgtttg actcagaaga
    30121 tcatatggta cggatcgata tgagtgaata tatggaaaaa catgctgtgt ctcgtctagt
    30181 cggagctcct ccaggctatg taggttacga agaaggtggt caattaacgg aagctgtcag
    30241 acgaaatcct tatacaatcg tcttgttgga tgaaatcgaa aaagcgcatc ccgatgtctt
    30301 caatatcttg ttacaagtcc tagatgacgg acgcttgact gattcaaaag ggcgagtagt
    30361 agatttcaaa aatacggttc tgatcatgac cagcaatatc ggttcacagt tactgctgga
    30421 aggcgtgact gctgatggaa cgattccaga agcagtggct gaacaagtca acacgctatt
    30481 acgtggaaac ttcaaaccag aattcttgaa ccgaatcgac gacacgattt tatttacacc
    30541 attgagccta gacaacgtga aaggaatcgt tgacaaaatg gtggcgcaat tggcacaacg
    30601 tctggaacat caagaaatcc tgctgacaat cagtgatgaa gccaaaacgt ggattgctga
    30661 aaatgcttat gaaccagcat atggtgcaag accattgaaa cgatttatca ctaaggaagt
    30721 agaaacacca ctagctaaag aaatcgttgc aggacatgtt atgcctaaat caaaagtaac
    30781 gattacttta cttgatggac aacttcattt caaaacagaa gagctagaag aaatcgtcta
    30841 atcttccatt gagactataa aagaaaaaag gggtctgaca caactagtgt cagacctcct
    30901 tttttcaaat aaacagtgtt ccagaagtag tttcttcgaa aaataagccg aaatttcttg
    30961 aagctgcccg cttctgtcac aacctctttt attaggtatt tttcactttt tctaatttat
    31021 aactttcaaa gtaacgctca tcttttttac tttgagacca tgcaaacaat cccattaagg
    31081 cagcacctat cgtatttgtc agcaagtcgc ccatcgtatc catcaatgct gctcgtccga
    31141 caaacagtgt gccatcagaa gcagcaaaac gttgaagatt catgcctaag aactggtcgc
    31201 ataagaattc ccagaactcc caaaacacgc cgcaaagtcc agcaaacgca aagccgaata
    31261 agaggaacaa ccaaggactg gtttttgata tctcagcatc tttcatcaag taaccaatca
    31321 atccatagcc aagagctgtc aggaccattg gactcaccgc atgtaagatt ttgtcccaaa
    31381 aagagatgat actgatcaaa tgcatccctg tgcctaaaaa gacagagatg aacaagaaaa
    31441 accagtaaaa caaaacgatt gctgctggta ttttaagttt gaaaactttc aaaatgactt
    31501 gtggcatgaa aatcaatgca atccctaata aagattccac gataaatgtg attccttgag
    31561 aagtcgaata tttttgtgtg ataaattcat aaaaattata gcagacaacg aatacaccaa
    31621 aagcaagtaa agctatttgc acctttttga tttctttaat cactgaaagt ccctccatct
    31681 aaaaatcttt ttacttctga aagcaactca atttctgaag aaaccacttt tccgttcaat
    31741 ttgatcaaac ctattgtata taagttcagg tagtgaaatt gattttcagc tgtttcttgt
    31801 agtgcagcga tttttttcgg gtgggttccg ccttgctgtc gagtatcagt atataagccg
    31861 atcactggga tgcctttcgc ataagccaca ccgatttccg atgctacccc ggcatcgatc
    31921 gtcagcccat ctagtaaagc aatcattaaa tcactagcca acactttttc tgtgtcagca
    31981 agagcaatca ttcgactgtc tgcataagca gttttgtcat tgattgcttc attttcttga
    32041 ggaagataga tcgaaagctc agaagagagc tctctgattt ctttaactaa ctgttggtta
    32101 tataacaaat cacttttagc aaacattggt gcggcaaaat aaattttcat ctaaacatca
    32161 tcctttttat caaaaataat cgctaaagta agcttccatg tttggaatga tacgctctag
    32221 tgacttcagt gtttcttcat ctgtttctaa ccgttcgatt cttgccaaat aagaagcacg
    32281 gcgataattc atcatcaagc aagtcaatgt ttggatatcc agtcgaacgg attttcctat
    32341 aggttcattt aagacacgga cttgtccctg ttcatcccat gttaaagcga agacaccatt
    32401 attccaagga gcgacaggat cttcaatgat gaaatgaaaa gcatcagctg ttccaacaaa
    32461 cggaaaccgt tgaaggaatt cttttacatc aacgatccga gccataaaat atggttcgat
    32521 ctgctctttg atctgactat cttcaatcaa aaatgctaaa ggctcatttt tataaatatc
    32581 tccatgtacc cagtagatca tggaaaaatg tgcggaaata aagttccata agccatttcg
    32641 agcttcttga tcaagataga acatttcttt tacgtgaaat atttcttcag ctacccagta
    32701 gaacagaaca cctcttggtt catggttgct atcgtagtag acagcggctg tgcgctcatc
    32761 ttcattttcg aaacgccaat actcttccca atgaaaatca ctgcggaata aagcaccatg
    32821 gttttgttgg gcaaatttgt cataaacgga aaatacgtct ggatgatcaa ctggtaatcg
    32881 ctcaaccata ccacttactg ggatttgttt aggtagctgt gtatctctga ttttaaagga
    32941 aagtttatca gacatgattt cccatccttt acgccgatag taggggatac tgtaaggata
    33001 caaataagag atccattgct tattcttgcg catattatcc aatgcaacga taattagatc
    33061 ttgcatcaat ccatggttag catattctgg ataagttcca actccagtca caccacccat
    33121 tttataacgt gcaccgtgaa tattgacttc acaaggatag atagcgattt gagagatcag
    33181 tttattttcg tggaaccatc cgaacacttt cgatacttcc aaaatcggtt ttttggaacg
    33241 gataaaagct cgtttattct caaacccgct ttcctcgatg tctgcttcag tcacttggaa
    33301 cacataggaa agcaattcat tgaattgatc gatatgttct tgagtgaccg gttttaattc
    33361 aagattttct ctaaaatctt tttgatccat tttttcatct ccatttatta gcttgataac
    33421 agtatagcaa aattctacaa aaaaaacttc tatagctgac taattaaaaa ctctttaaat
    33481 tactgctttt cgttggtata atggaaagtg ctgattatat gaaaagagga gacgctatgg
    33541 atataaagaa aatgaaagaa cgccaagagc agattcgtaa tttttcgatc atcgctcata
    33601 tcgaccatgg gaaatctaca ttggccgatc gtattttaga aatgacgcac actgtgacgt
    33661 ctcgcgaaat gcaagatcag ctgttagact cgatggattt ggaacgtgaa cgtggaatca
    33721 cgattaaatt aaatgctgta gaactcaatt acacagcgaa aaatggagaa acatatatct
    33781 ttcacttgat tgacacccca gggcacgtcg acttcaccta tgaagtctct cgaagccttg
    33841 ctgcctgtga gggtgccgtt ttagtggtcg atgcagcaca aggaatcgaa gcacaaacat
    33901 tggccaatgt ttatttagcg ttagacaatg atttggagat cctgccagtc atcaataaaa
    33961 tcgacctacc cgcagcagat cccgaacgtg tccgtcaaga aatcgaagac gtaatcggaa
    34021 tcgatgctag tgaagctgta ttagccagtg ccaaatcagg gattgggatt gaagatattc
    34081 tcgagcagat cgtggaatat gttcctgcac caagtggaga cttagatgca ccgctaaaag
    34141 ctttgatctt tgactctatt tatgatagtt accgcggtgt tgttctaaat gtccggatca
    34201 cggacggtat ggtcaaaccc ggagacaaaa tcaaactgat gagtaatggc aaaacatttg
    34261 atgttacaga ggtcggggtc ttctcgccaa aagcagttgc tagagacttt ttgatggtcg
    34321 gtgatgtcgg ttatatcaca gcaagtatca aaactgtcca agatacacga gtaggggata
    34381 cagtaacgtt agcagacaat cctgccgctg aagctttacc aggttaccga aaaatgaatc
    34441 cgatggttta ttgtggtctt tatccaattg atacttctcg ttacaacgat ttgcgggaag
    34501 ctttagaaaa actgcaattg aatgatgctg ctttacaatt cgaaccagaa acctctcaag
    34561 cattaggttt tgggttccgc tgtggattcc tcggacttct tcatatggat gtcgtccaag
    34621 aacgcttgga acgggaattc aatctagaat tgatcacgac tgcaccatcc gttatttacc
    34681 atgttaataa aacggatggt acgactgcta cagtagataa cccggcagat tttcctgaac
    34741 ctgtaacgat tcaggatgta gaagaaccat tcgtcaaagc acagatcatg gtcccaaatg
    34801 attttgtcgg tgcagtaatg gaactttctc aacgaaaacg gggagaattc attacgatgg
    34861 attatttaga tgattaccga gtaaatgtcg tctataatat tccgctttct gaaattgtct
    34921 ttgacttttt cgacaaattg aaatcaagca cgaaaggcta tgcctcattg gattatgaaa
    34981 tgtctggtta tcaaaaaagc aagttagtga agatggacat cttgctgaat ggagaaaaag
    35041 tcgatgcatt aagctttatc gttcaccgag actttgccta tgaacgtgga aaagcaatcg
    35101 ttgaaaaact gaaaaaacta atcccacgcc aacaatttga ggtgccaatc caagcagcta
    35161 ttggacaaaa aatcgttgcc cgttcagaca tcaaagcctt gcgtaaaaac gtcttggcta
    35221 aatgttatgg tggagatgtt tctcgtaaac gtaaactctt agagaaacaa aaagaaggga
    35281 agaagcggat gaaacaaatc ggctccgtcg aagttccgca agaagccttc atggccgttc
    35341 tgaaaatgga tgagcaagat agtgtgaaat aacttataat caatcgaaaa acctattaaa
    35401 taaacctttt tgagtagatg caacactgag gattatctgt ataaaagatt ttaaactgtc
    35461 atttggttat cacggcgata ctaaaattaa caaaattgga atatttttct tgaagatttt
    35521 tagctgtcag tatatgatga aagcatctca taggaggtgc ttttttcatg gttgataatt
    35581 caagtggaag ggttcttaaa tctctaagaa aagagaaaaa actatctcaa aaaaagttag
    35641 ctgatttagc aggtatttca caaagtactt tagttaaata tgaaaaaggg tcaagaaaaa
    35701 tccctaaaga cgtagataat actctaagca agattctgaa tatagaaact ctaataaaag
    35761 atgaggaaga taagattgag attctcatcg gtaaactaat tgcatacaga gatatgaata
    35821 agttacttaa caaagagtta gctgataata taggaattag tgaagtatta ctcagttatg
    35881 tgttaaatag aaagagaaat ccgtcaaaag aaatgcaaaa aaaaatcgat atttttctat
    35941 taagtaatga aaaagaaatt ttgaaggaga ttaatcgtga tagtgagata tttagtttat
    36001 caaaagacga taaaattgtt atgggaaaaa gaatacgaga agtccgaaag aaccgggaag
    36061 agacgcttga aaaatttggt aaaaacttta ccatctatac aggcaaaaat gttataagta
    36121 gatgggaaaa aggtattaat attccagata ttgaaaaact aatgaatatt gcctatcttg
    36181 gtaaggtcac tgttccttac cttatgtacg gagaagatta taagaatata cttccaaaag
    36241 atgaaagagt tagcgatttt aaaaagataa attcttttag tatgggatta aggatgagga
    36301 aaattaggaa agattattat ctagaaaggg aggagtttgg aaagcttttt tctccttcaa
    36361 tttcaaaatg gtccatagat aggtatgaga atggtagaga tatacccaat actaatcgaa
    36421 ttatccaata tgcttatata ggaaatcttt ctttagaatt tttgatttac gggatataaa
    36481 aatagtttac gtcgatactt attaagttat cgacgttttt tattatagaa aaaatttata
    36541 aaaggaggtt tcttgatatg ataagaaaca aaaagggttt gttgaaagtt gctttactgg
    36601 cagctttttt ctaagtaact ttttgacaag ctcaatggca acacaagcgg aagaagtaat
    36661 acaagaacta actgttatga ataacagcta gataaaatgg gctatgtatg ttatccaaca
    36721 atttagtaat cactagaagc ctagcgaaag ttcgcttgat aagttataga tgagtattga
    36781 tttgtagtgt ttccaaatca aaagggtaca gagccacatg aaatatgttg tcctctgcag
    36841 cgtcaggatg ggaatccata ccatggagat tcggagatct gtgagggaat acttacgtgg
    36901 ttaatatgtc agttgtgttg gacgtagtaa tggtcatagt gggtagattg actgaattct
    36961 tatatgagaa aatgaaacca acaagaagat agggttattt atacagaatt ggggatcttt
    37021 tctggaaaag taatcggata atgcgagatt cgtacggtct tttttaaatt tctctaaaaa
    37081 aaacggcatc gaatgggttg ggtgattgat aagcaatgaa tcatagactg atatagaata
    37141 aaaaatgatt ctatttatct ttttacatct atcgtttttt caagtagccg caaattatat
    37201 cagcattcaa caatttttgt atacaagttc tagtaaaagg atctgaagaa aaagttggtt
    37261 gttgtgaaga taaaaaaaga atcaggtttt actgagtttt tcttcaaaat ttgtgttatt
    37321 aggttaggac aaagataaga agaagtatga agatttaaaa aaggaatgag gaaaagatag
    37381 taatttccga ttattggagt ttttttattt ataaagaaga agcgaataag ttgtgattcc
    37441 tagatttcgc gcgtgattaa ttaatgagca aaaaatgcgg aaagttttag tgtttaatac
    37501 acgaggacaa agtttcttag gtattgaagg cacacgtgta acggttgatg ggtggtgtga
    37561 ttgtttcgag gttggaaaac atgttgaact tatggcattt actgagctaa aagataagaa
    37621 caacgtggaa tttttcgaga gtgatattgg ttgggacgat catgaagaag tttatggaaa
    37681 atttattttg cggaagggaa attcttatac gaatggggaa gcataagtag cgatttgtta
    37741 gagatcattg ataggattga aattgtcggg aatatttatg agaatctaga gttatttgag
    37801 gtgatagaat gagtgatggt tttgaacaag tatcaataat taaaaatcaa gttcgggaga
    37861 ttcttagaaa aaaaggctat ttggtagata gttattttga aggagattac gaaacatggg
    37921 taggtgtcta tgctcgtcca gagaataaac ctacctattt agatcctacg acgtcagaag
    37981 atgcctattt acaaaatagg tatcgagtag atggttttaa gcaagacttt gcggagtggt
    38041 ttgaatggga aattgaaaac ggagaagtaa aagaagaata agagtacttg ttgtatcaaa
    38101 ataagccagt agaacaacca aaccagtagc tagtaatgac aataatactg aggaatcgga
    38161 tcattatggt atgattgata tttctcttcc agaagatctt aagccagatc ctgtaacaat
    38221 tggcaatagt gtcatgattt ttgattcatt agatgaagct atgtcatatg gttggaaaga
    38281 aattaaggat gaaggtagta attggtatgg catgagattt ggtgatggtg aagttgccaa
    38341 aaaagctggt attccaattg ggaaatggac agttgatttc cacgaagcag gttctttttt
    38401 tgttctgtaa ttatagtggg agggtaaaat cattgatgaa atgtgttcag ataagagcgc
    38461 cagataaaaa tagaagagac agttcacgtc gttatcactt ttggtaacgg cgttttgttt
    38521 tattatataa aattctcagg aaaaggagga attcttgtgt atgcaaaaac gaaaatgaat
    38581 tggttgaaag cactcgcatt ggtggctttt tctcaattct gtttcttggt gggaactgct
    38641 caagcacatg cagaagaaac gacggctata caacctacga tggaagccat tgcccaagtc
    38701 gcttctgaag tgacagaagt ccaaccagca gaagccgggg tcccagctgg tgaagccact
    38761 gttgaacagg cccctgtgac agaagcgcca acggtcaatt ccaaaacgac tctcgatcag
    38821 acgattgggg aaacacaaca cccagggaat agtcctggta cttcacaacc agttgaagaa
    38881 actgcctcaa ttacaacccc tgcttcagaa acgtccactc tagaaggaga aaaagctgcc
    38941 gaagcaacgc cttctgtgca gccagagtct tctgagaaac aagcggaaga aaagaaacga
    39001 atagaagaaa cagtggaaca accagcggct actgctgaca aagaaggaaa aaatgaggaa
    39061 aacgttgaaa aaacaatctc ttcaaccgca gctagtcaaa atcaaccaac gaaaccaaac
    39121 aaagaatcta cggctagtat tgaaaatcaa aagaaaaaag agcagaacat tccaaaaaca
    39181 ggtacaccta cactgttagt aacagtacca accaatcgtt cggttcgtgt tcgccgtgca
    39241 accaatgcag ctgttttagg tggtcggaaa attgattggg gcgaaaatat tggtgttact
    39301 tctagtccga acaatctaaa cttcacttta aattatatgg ttcgttttga gattaatgga
    39361 caagtggcgt tttgtattga gcctggtgtc tcagccgcag aaggtcaagt ttatacagct
    39421 actgctttga aaaactattt gaaaaattca aatgtacgga aaaaaacaag cttgatctct
    39481 tatttaggat atgtcacaaa taaagacaaa tccgatgaac aatacattgc ggcacaattt
    39541 atgatttggg aagctttggg cacaaaaatc ccaatcaaaa atatccgtgt tgattatgat
    39601 cgtcgaaaag cagaaattca acgtttgatt aataactttg gtgaaaaagc aacttttaat
    39661 aatcaaacac atacggtaaa aattggcgaa acgttgaaag taaagaataa aaatgatttt
    39721 ttgaaatatg tgaaagacat tcaaaaacca aaaggtgtca atgtatctat tcacggcaat
    39781 gaagtatgga ttacagtcac aaaagaggca ccggataaag caaaaattca attgaataaa
    39841 attaaagttc aaggtgcgcc attagcctat cgtcgtccag gagctcaaac aattggtgtg
    39901 ttacatccaa cagaacctaa tgcgtctgtt attaatttac aaattctaaa acaaggaaac
    39961 attcgtgtgt ttaaagaaga tgcggaaact ggtgtgaaag cacaaggtgc agcttcttta
    40021 gaaaatgcgg tctatggttt gtatcaagca gatgggaaaa agattaaaga aattaccttg
    40081 aaaaaaatta acggcaaagt acaggcagaa attaaagatc ttgatcctgg caaatatgtt
    40141 ttaaaagaaa tcaaagcgcc aaaaggttat gtgctttctg atgaagcaat tgaaattgtt
    40201 gttgaacctg gaaaaactgt caatgcgacc gcaaaagatc aagtgatcaa aggaaccatt
    40261 gatttagtga aagttgctaa taaagattta gtcgattcaa cgaatccaga taacaaacca
    40321 aaattagctg gcattgaaat cactttgacg tcaaaaacaa ctggcaaagt ggtgaaaacc
    40381 gttgtgacag acaatgatgg ttatgctact tttggtaaaa ataccgttgt ctttgatacg
    40441 tacattcttt ctgaaaccaa aggaaaagaa gggtataaac tatttgaacc acttgaagtc
    40501 acaatttctg aaaatgggca aactttccat tatgttttag aagataaagt aattgaacaa
    40561 cgtcttaaag tggtgaaagt ggatcaagaa acaggaaaag tgattccact tgctaataca
    40621 gaattcaaga tttttgatac tttagccaat cgttatgtca caatggagat tccaaatgat
    40681 acagaaacaa cggatatctt taaaaccaat gaaaaaggct ttttcctaac taacggtaca
    40741 cttacttatg gaaaagatcg ctatcgtttg gaagaaatca aagcccccgt aaattacgtt
    40801 ttgaatcaaa acccattagt gttctcgatt accaatgata cagaaccgat caaagtgatc
    40861 cattttgcga accgtcaagc acggaaaagt gtaaaattat tcaagtatga agaatggaac
    40921 aagaagaaaa cgccgctcgc aggtgtgaac tttaccttgt ataacggtaa aaatcaagca
    40981 attggtgaat ataccactga tcaaaacggg gaaattttag tgaaaggtct agttgccgat
    41041 gactattact tcttggaaaa tgccccatta gaaagtttcc aaccaaatac agataaacaa
    41101 gcattcacgg ttgcattcaa acaaaatgcg ccagatgatg gggaaacgtt agtggtttct
    41161 gtacgtaatt acttgattcc accaaaacta gaaacacaag cgtcaaataa agccgatggt
    41221 gaaaaagtgg ttgatccatt agaaacgatc gtcattaaag acagtgtttc ttataccaac
    41281 ttgtttgtcg gaaaagaata caccatttca ggagtattga tgaacaaggc aacgaatgcg
    41341 ccaattttac aagatggcaa agaaatcaca agtaccttgg cctttattgc agacaaagtc
    41401 aatggcacaa aagaccttga atttgtggtt aatgctagtg ttctgaaagg tcaaacgatc
    41461 gttgtttttg aaaaactttt ccgtgatggc attgaagttg cagcacatgc ggatatcaac
    41521 gatgaagccc aaagtgtgaa agtgaataat ccaaccgttc ataccacggc tgtctttgaa
    41581 gacggattga cagtcgcaga cccaacagga ttgtttacct taaacgatac cgttgaaatt
    41641 accgagattc ttccaggaaa agcatacttg gtcaaaggga tcttaatgga taaagcaacc
    41701 ggtgaaccct tgcttgtgca tggaaatacg gtagaaacga gtgttggctt tattgccaaa
    41761 gcagaaaatg aaacggtgat cgtaccattt acctttgatt tgacaggtat aaatggtcgt
    41821 gaaatcgtcg tgtttgaaag tctttttaga aatgatgaag aactagcaaa ccataaagac
    41881 atcaacgaca aagggcaaac ggtacgagta actaacccaa gtgtacgtac aacggctaca
    41941 cataaaccca ctggcttgaa agaggttaat ccattaacga aagtcaccat tacagacgct
    42001 gtgaaatacc acgatttggt cgttggaaaa acatacacag tcaacggtgt tctaatggac
    42061 aaagtaacgg aaaaaccagt gctagtagat ggaaaagaag tcacaagtac cttaacgttt
    42121 gtggcagaaa caacggatgg tacagtcaat ctagacttta ttctagacgc acgtgcgttg
    42181 cgaggcaaag aagtcgtggt ctttgaagat ctgtttagag aaggcgtatt attggcttct
    42241 cacgcagata ttaccgacaa tgaccaaacc atcaaaatca gtaatccaac cattacgacg
    42301 aaagcgacga ataaaaatgg cggaaaagag atcttggcct tgccaaatca aactgtgctt
    42361 gaatgggtca aagtagacgg tcttgcgatt ggtcaaatct atcgcttaat tgttcaaggc
    42421 tacttaacac cagacggcac accatttgaa ggcactcatg cggaaaaagt ctttgcggcc
    42481 gataaagaaa tgatggaatt cttatttgaa ttcttagtgg acggtcgcaa tcttgcagga
    42541 aaaggcttga gttttgcgga atggctccaa gcgaaaacgg aagatgagaa agaaacgttt
    42601 gaagaagtgg cgaaacacaa tgtagatcta acaaacaaag atcaaacggt tcaaattgtg
    42661 gaagcgccaa aaccaccgca accagtgaaa ccagaacaac gggttaaacc agctactgtt
    42721 gtaccaatgt tgccaaaaac aggtgaacaa acaagtagta tacttatggt tcttggatta
    42781 gtagtattag tcattgcttt aggtataaca ctttataaag tacgtaaagc caaagattag
    42841 tcatgaggaa caagcggtta ggatttttaa ccgcttgttt gattgtaatg tagaaatatt
    42901 tagaatttat tttggttttt aagaggagga aaaactttat ggcagaaaaa gcattgcgta
    42961 tgaagaaaga gtttttaggt caagtatttt tattgggttc acgggaacct aatccaccaa
    43021 aggaaattaa tggtgtgtct gggcgatatg ttcaattaca agcagagtgt tcgcgagaag
    43081 aacagcctgc tttttttgtt tatctcacag ctgaagagga taaaagatta cgattaccag
    43141 aaaaagaggt gcgtatttca tttgaagaag cttattttgt ttttcgtaaa gaacgagtga
    43201 atagtcgtga aacaaatgat atgggacaaa cagagcgtcg aacaactggc tatcaagatg
    43261 ttggtgtctt tttaactggt gtaaaaattg gggaggtttt gtaagatggg attaaaattt
    43321 ccaaataatt atgacattac agaagcatta gatgaaactt gtttaggagc cgtacgcttt
    43381 ggcgcattag aagagtctga tcgtgtattt gataatgaaa caggcgatta tacgggagaa
    43441 attcgatcac agcgagcaca attagttttt ggaaatccag atgaaccaaa gattgcacga
    43501 cgagttaaat ttccagcaga tgtagatttg actggtttta aacgacgtga tcctgtgaaa
    43561 ttaattgatc cagaatatca tgtggtatat attccagcac aagatggcaa tcgtgaatat
    43621 tcagatttag tgatttatgc gaaaggatta gagaaagtag aagctccaac aaataatcct
    43681 aatatgaaac aatcacaaga aaaaggttta gatcaaaaac aaccaagtgt tgattctaag
    43741 gaaaaagaga aaaaataagg tttttttgaa agaataggaa ggagtcttcc tattcttttt
    43801 atctaagata gaaggaaggg gagattttga attcagataa aaccaatcaa atactttgag
    43861 agattgtgct tgttatcaaa atatccaaca attgattgag tattttggag acaatccaac
    43921 gatttttgta caaacaaatg catatatttg tgcggttatt gggcagtgta tgtttttacg
    43981 tgatcttgtt cgtttttcat taccgtacca gtggaaacaa gatcatcagg aagtgatttg
    44041 gattcgtata gtacgaatat gtaatatgtg tgcacaccct tataaaaatc gattaaatct
    44101 ttttcgatta tgttttttta ctaaatgtga tattcctgta ttagttgctt tttgtcaaaa
    44161 gtttttagca caggaaaagt aaaaacagaa aggaggaatg tgacaattga aagctcattt
    44221 tttcaagtat cgtggtcggc gaattcgcta tttcaatcgt tatttatggt tttgctatta
    44281 tggtttcttt ttgtttccat ttttgattct gttaggttgg tttagttgga aagtatttta
    44341 tccacgttta agtacgtttt cgaattggca aatgtatctt attcctggaa taagtattgt
    44401 gttgtatttg ttgctggtta gtgggttaat tttagggttg atgcattggt cgcgactaaa
    44461 agaaggatat tttgcctctg tttattggcg tcagttactt gttcgaatgt taattgacaa
    44521 tcggtttgtt tatacaaaga aacaagttag tgagaaacaa acaagggaaa aaatgagatt
    44581 accaaaagtt tatttttatc aaaagaaaca agaattagtg gtttcttttc ctttagatgg
    44641 tgggcgtttt cacgaacgat ttttaaaaat gggtcatttg ttatctgaag tatttttgcg
    44701 tgatttaatt cgtgaagaac gagaaaaagc ccgaatcaat tatgtattcc tttcagattc
    44761 tggtcggatt cctattgatc aatgtatagc agaaaatgga cgtattcatt taatgaaaac
    44821 tttggattgg gtatatgatc aatcaccaaa tatgttgatt gctggtgcaa tcggtggtgg
    44881 gaaaacctat ttattgtatt ctttaattca agcttgttta agtgttggga cagtagatat
    44941 ttgcgatgga aaagcggcag atttagcagc attaggtgac gtcgatgtat tcaaaggaca
    45001 tgtgttttac aaaataaatg aagaaatgat tatttgttta cgaaatgcat taaaagaaat
    45061 gaatcaacgg tatgcctata tgaaaaccat gaaagatcct aaatatgaac cagggaaaaa
    45121 ttatgcttat tatgggttac caccgcattt tatctttttt gatgaatggg cagcttttta
    45181 tggttcgttg gatatggaag cgagaaatag agtcgataaa ttgattcagc cattagtatt
    45241 aaagggtcgt caagcaggga tgtacattat tttagcaatg caacgaccag atgcagaata
    45301 ttttccaagt ggtgttcggg ataatttaac attccgtatt tctgttggtc gattgtctcc
    45361 cattggttat ttaatgactt ttggagaaga ttttaaaaat aagccatttt acaacaaaga
    45421 tcaacctggt cgtggatatg cttttccagg atttgatgta cctgtagagt tttttgcgcc
    45481 atttgtttac aaagattatc actttatcaa agaatttgag aaatttactg aaatgattgt
    45541 ctatgatttt acagtaaaag aggatagtca agatcaaacg atgggagatg ataatcagtt
    45601 gttacaagaa caactagagc gccaaatgta tttagcaaaa gatccaaaag gaacaagttt
    45661 ttctgatgcg catattttgt aatcaactaa ggaggtgaag ttaaatggca gagagttgtt
    45721 attatagagt agctacagca atacgaatga ttaatccttc tctttcttcc agaacgtttt
    45781 atgattggtt gaatcgtatt gaacaagtaa ctgattaccg ctttttgcgc aaagaacgtg
    45841 tttttacagg aaaagttatt aatcaagtat tgctaacgaa aaaagatata gaacgattga
    45901 ctcgattgta tcattatcga gtagatttag aagaggattt aactttgtca atttatcgcg
    45961 ttttttctcc tgaaaaatat agtgaaataa caaaattaga tcatttaatt ttgtagttat
    46021 tatctatcgg gagggctcta gacgtagtct ggtagccgca ctctgcctgg cggtgcgcat
    46081 taaggctgcg aagcatgggc ctataatgcg cactcgctag cagcgtgtgc agctcacaaa
    46141 gaggtcgtgc gcttgcgcac ggcctctttg tgtaaagccc cccgatatat aatatggggg
    46201 gcagaaataa cataacacat agaaaaaagt tttataaacc cttttgtatc aagtgattat
    46261 ctaaggtcag cagttgtact acttttccca aaaaacgagg gggactagtt gatatatcgg
    46321 tgtttctaaa gggttaaaaa aaatcgattt tgttatttta taaaaggagc gagaaaatga
    46381 caccgattga gctaaaaagc tacagaaaga agttaagtgc aattcataat ataagtggag
    46441 ataaaaagcc gctatcacaa gcaagatttg cgcaattgat gggctgctca aaagattaca
    46501 ttagaaagat ggaacaaggg aaaagaaaaa tcccagagga ttttgaacaa cgaatcaagg
    46561 aacgctttcg attgtatatg aaaagcggaa tagatcttcg agcaatgatt gattatttgc
    46621 gtctttcctt ttttgggaca gactacaaaa cagtgattca acgtgtatta cgaatggatc
    46681 tgacagaatt tgaaacacgt ccaacgggct tgtatctttt tgactgtctt tctgttcgtg
    46741 ggaatatctg ggtgttctgg cattcaaaac gaactgtaac agaaaatgta cttgtgcaat
    46801 tttcaggaca aggttgccgg gaatatgagg aagtcatgcg tgaacaacaa acagattggc
    46861 gaacatggct atattccatt tggaaaaatc aaggaaagtt ggaagctggt tatacacgtg
    46921 tgcaatgtag ccgattcgat attgcgttag atgaactttg gggattggaa acggaagaac
    46981 atttcgatct ctttgaatta ctagaaaaac aaaaagcagg tttattagaa atggacttta
    47041 agagattcca gaatattggc ggttgttttt tagatgatgg ctattttcga agtgaaggat
    47101 tatctttgta ttttggatcg cgaaagagtc ctctcttttt taatttttat gagaaacgtt
    47161 ttgaaattgc gaaccgagaa aaaatttcag aattagaagc attaacgaaa tatggtattt
    47221 ataatcgata tgagttacgg ttagccaatg agaaagcaac acaagcagta gaagcattta
    47281 ttttaggtga ttcaccgaaa cgtttaggag agattggcgt tggtttaatt aatgcaaaac
    47341 ttattgtttg ggatagactg gaaagtgatg cacggattgt tgataaacga tgggcaaaat
    47401 tatttcgttc gatggaagaa ttggatttta cagtaaagcc agaaaaattc tcattagatc
    47461 gttcagaacg ctggtttttg aaacaagtag ctccgacatt aaagttgtta caagaagcag
    47521 atcgtatttt aggaagagac tttgtacaag aagggattga taatgccgaa ctaaatgacg
    47581 agcaacaaaa ctatctagaa tggatgggaa cgtcaggagg gaaaattgca tgaatgcata
    47641 tacgacaaca gaatggctgc gcctgttgga tttaaaggct gcaatcgaag cattaaatga
    47701 aaagatggtt gatttgtctt attttcgttt tcgagttcct tatattgaac aagcggtaaa
    47761 agctggacgt tatcaggaaa aagagaactg gcaagaaatt gcacgcttat tagaagttcg
    47821 taagggatac gaacaagaac tcgaagagtt ggaatttagc cgaaggaaag gaaggctaga
    47881 attcattcgc ttttatcgat tttcattgcc aatacctgcg attttagcgg ttaaaaaagg
    47941 ctgcgataaa atgaagatat atgaaaactg tgtggcagca cttagcagcg aaaaacctct
    48001 tatggaagaa atttcattgg tgactgtcac ttgggaaatg agtcgccagc caacagaaga
    48061 acagacatat ctttctttag aagagattga gatagaatta gaggaaattg gtcgctatgc
    48121 cacttgttct acctattgtg gatcggtcat tagtattgct ggtgtgattg tctaaatgaa
    48181 aaaaattatg atttatgttg gtgcgtattg gtcacgtgat ccaacagtat tagaaaatcc
    48241 agaggcggta cactattgct tgcgccaatt attctatctg tataaagaac gattagaatc
    48301 gttgattcgt cagttaccat atactgacag acgtttagat gaacttcttc taaggtatcc
    48361 agcaatgtat aaacgacgaa aaaatagatt gttaccagaa gagtatccca ttgaaaaaca
    48421 tgaacttgaa gggcgatttg tggcttattt ctatgatgat gttcgtatgc gcttagtaga
    48481 acaacgaatg gaaatagaaa atgatcgcta ctattttatc aattattgta agaagcgtaa
    48541 gtatcaagta actgatgatt tttatgattg tattttacaa gatggggaag tgattctatc
    48601 caaaattgct ccaagttttc gtgaacttgt gccgattgat tttttgaaaa aatgtcattt
    48661 gcggattctt ccgtaaaaaa gagctataaa aggaaagaaa agtagaagcc aaatcggttt
    48721 ctgctttttt atttttttta ggaaggagaa gatggtatgt cactggaatt tttaggagag
    48781 ccaattgaaa aggaagagtt cgtttcgcat tatatggatt tgtttattca ggtgataaaa
    48841 aactttgtgc gattgatgaa ttttatccac gcgaacaaga ctatctagcc gctgaacgaa
    48901 taaaaatcag tttattatgg gaaaaattca cagatgaaaa taaccgaccg ccagattatc
    48961 gctatttatc agattgttta acgaataatg tgctttctaa agaataccta tttcacaaac
    49021 gtgagaaaaa tcaaatgtcg gttgaacatt tttgtgattg ttatattgca ttattgcgta
    49081 gtcaacagtt attaacagta gaacgatatg gtgcagagtt tggttatgtc atgcaagctg
    49141 agcgtcatag agctacaaga tgctatgaaa aagaagatca agtgattgat ggctatgaag
    49201 aattatgtat tcgaaacaat cgctatttgc aacaacgttt atatgaacaa ttgatgatgg
    49261 atttttcaaa tctctataga gggtatgaat gttcgatgat tcaagaaagg tagaagaaaa
    49321 gggggaattt ttatgtggcg attattattt ttggtggtat gtggtatggc attgttctat
    49381 gtgtatcgtg tctatctcta tccacgaatg gaacgatgga caagttggta tagagatcct
    49441 ttaaaacaac gaaaaggatt gaagcggtat cgttatgaat tacttgttga attttcggat
    49501 gggcgacaag cacagtatta tttttttgca aactatccaa aatacaacac cagtgaacta
    49561 ttgattcaat tattcaaaaa taatccatat gttgggttag aagaaaacgg acgggagttc
    49621 ttttatcgtt cagataatat tagtgcggcg tctatgaaca aaacacgaat tgcataaaaa
    49681 attggaggaa taatcatgaa aaaaacaaca ttagcagtcg taacaacact aggaatttca
    49741 ttattttcgt ttcaattagg gagttcattg gtttatgcag atgaaactgt agaaacacct
    49801 aagtcagaaa ttgtagaaac attgccaggg gaaggattac cagatgttga gacagagcta
    49861 aatggaggaa caacggaaaa accagcagaa cctgaacaat tgcagtcaga gaaacctgaa
    49921 aaagagccag tgacaccaga accagagttt cctgaatcaa ctttaccgac tgattcgatc
    49981 gaagaagaag gtgtgcatag ttcaacaaca acggattcaa gtatagtcac aactccggaa
    50041 caatcaggaa caaccagttc ttctgaaaag aatactgatt cgtcaagtac ggaacagcca
    50101 acgatgtcgt ctaccagtga agaacaaaaa gaagaaccga aacaacctgt tgaaaaatca
    50161 gaagcaattc caaaagaaaa taaacctatt gttccagcaa aagaagtcac agtatcggta
    50221 accccaagcg gtgaaattac gacgaatact tctcaaggaa tgagtgtacc gattgtcact
    50281 agtaatgtag aggaattgac ccatattcca acaccaacca ccccattaaa agtagcaggt
    50341 gatcaaacaa ttgttggtgt aaaagatggt attccattaa tccaagatag tgaagggaat
    50401 ttggtaaagg atttgtcgat tcctgtaaag aaacttccaa gtggcaatat tgaaatcaaa
    50461 acagcagatg gaaaaacaaa agtcttacca aagactggtg aagaaataca tgttgcactt
    50521 tctgttctag gaggagtgtt gacaagtatt gctggatttg ttggatataa aagaaagaga
    50581 aaaggaatag aaaatgaggg taattgaatg gactattttt cagaaaaaca agcttatcgt
    50641 tgtcaatcgt acgtactagt aaaaacatac tagaaggagt agcttatgaa aaaacgacaa
    50701 ctacggcgga aagaaaaagt ttatacagag aaagtaccga agcaaaagaa aatccattca
    50761 aaaaaagcag tgaaatggat ttggtgtttg gttttatttc tttgtttttc agggggcttg
    50821 gcattttttc aatccatgaa aacgaaggtt caagtgtcgt ttttgaaaga tgaaatccaa
    50881 caattacaaa agaaagaagt acaaagtcgg tcagaattaa cgtacacacc tgaaattgaa
    50941 tcctttatga atcgttttgt tgctttgtat atgaatacat tcgataatca tgaacaagaa
    51001 caacaacgtc aagaaacatt aaaaaaagag ttttatgcac aaaactttat tcaagaacca
    51061 ggaagtgttt caggtgttcg aaaattaact gctgcttcgt ttattggatt aaaagaaatt
    51121 gaaaccattc caactgctag ttataaagta acttatgata ttcgctcgat gaatccagag
    51181 agtaagaaag aaacaagtgt gacaatcaca caagtactca atcttccctt ttctatttca
    51241 aacaagggct gtcgtattat tgcttatcct tattttacgg cactaccaaa gaatacagaa
    51301 aaacaacgaa tgattactta tgatgacaca ttatacgaaa cggttgatgc gtcggaagcg
    51361 aaagatgttg aaggatttgt ggaagatttc ttagataagt atgcgaaaaa caatgcaaaa
    51421 gatatgtcat acatgatgaa agaacctgaa ggactcaatc aaacagctaa agttgatcaa
    51481 gtagagatga aagtattgcg ttataaagaa acgaaaaaac cttttattgt aaaagcaacg
    51541 gtcgtctttc gagatggtga tacgtcactt ttacaaaaag aacaaatgac gattgtcatt
    51601 acgaaaaaaa caaatcagta ttatgttgaa aaattaacac ataattggtt ggataggaaa
    51661 ggagaagaac ggaaatgaca ctgaagggaa ttgtagattt tatattaacg caaggtggct
    51721 attttttaat aatggtggtt gtttatctgg gattgcgaaa ttttatgaaa tcaaaagtag
    51781 gaaatgcatt aatatctatc gcagcaggtg ccgcagttta tttcttctta aatgatccaa
    51841 cacgtgtatt tgatgcgttt ggtaaaatca ttgaaaaatt atttatggat taggaggata
    51901 gtgaagtgga ttgggaacct tatgattatt cgattgttta tgcggaacct tatcgcgtac
    51961 aacagattac aagcaattgg cgtcttccat ttccagttgc atta
//
LOCUS       DAZKKW010000011        49032 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.011, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000011 DAZKKW010000000
VERSION     DAZKKW010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 49032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49032
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.011"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..345)
                     /locus_tag="ACG8PO_000614"
     CDS             complement(<1..345)
                     /locus_tag="ACG8PO_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0205966.1"
                     /translation="MVLNNFLKEGFILSYLIVCLIIIICLHHLFLSKTIPRYSKNKKA
                     EKFCLLLNKFTLIAPILAFIIFSVLLSTTLKGKFMERSSHAMILTFLWLLFTRIYIFL
                     MSLKPPKSISLCL"
     gene            complement(642..3023)
                     /locus_tag="ACG8PO_000615"
     CDS             complement(642..3023)
                     /locus_tag="ACG8PO_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HFQ0205967.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLQQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEETGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(3013..3660)
                     /gene="recU"
                     /locus_tag="ACG8PO_000616"
     CDS             complement(3013..3660)
                     /gene="recU"
                     /locus_tag="ACG8PO_000616"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HFQ0205968.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            3724..4263
                     /locus_tag="ACG8PO_000617"
     CDS             3724..4263
                     /locus_tag="ACG8PO_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HFQ0205969.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            4353..4772
                     /gene="gpsB"
                     /locus_tag="ACG8PO_000618"
     CDS             4353..4772
                     /gene="gpsB"
                     /locus_tag="ACG8PO_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HFQ0205970.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            4794..5161
                     /gene="rnpB"
                     /locus_tag="ACG8PO_000619"
     ncRNA           4794..5161
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACG8PO_000619"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            5370..6536
                     /locus_tag="ACG8PO_000620"
     CDS             5370..6536
                     /locus_tag="ACG8PO_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HFQ0205971.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            6582..8078
                     /locus_tag="ACG8PO_000621"
     CDS             6582..8078
                     /locus_tag="ACG8PO_000621"
                     /EC_number="3.4.17.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HFQ0205972.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKSNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            8159..8842
                     /locus_tag="ACG8PO_000622"
     CDS             8159..8842
                     /locus_tag="ACG8PO_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFQ0205973.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            9487..10677
                     /locus_tag="ACG8PO_000623"
     CDS             9487..10677
                     /locus_tag="ACG8PO_000623"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HFQ0205974.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            10779..11732
                     /locus_tag="ACG8PO_000624"
     CDS             10779..11732
                     /locus_tag="ACG8PO_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HFQ0205975.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSGNVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            11801..13150
                     /locus_tag="ACG8PO_000625"
     CDS             11801..13150
                     /locus_tag="ACG8PO_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFQ0205976.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(13333..13656)
                     /locus_tag="ACG8PO_000626"
     CDS             complement(13333..13656)
                     /locus_tag="ACG8PO_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0205977.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            13785..14948
                     /locus_tag="ACG8PO_000627"
     CDS             13785..14948
                     /locus_tag="ACG8PO_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFQ0205978.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            15018..16367
                     /locus_tag="ACG8PO_000628"
     CDS             15018..16367
                     /locus_tag="ACG8PO_000628"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFQ0205979.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(16412..16843)
                     /locus_tag="ACG8PO_000629"
     CDS             complement(16412..16843)
                     /locus_tag="ACG8PO_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0205980.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            17231..19873
                     /gene="alaS"
                     /locus_tag="ACG8PO_000630"
     CDS             17231..19873
                     /gene="alaS"
                     /locus_tag="ACG8PO_000630"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFQ0205981.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKEHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            20073..20669
                     /locus_tag="ACG8PO_000631"
     CDS             20073..20669
                     /locus_tag="ACG8PO_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFQ0205982.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            20791..21939
                     /locus_tag="ACG8PO_000632"
     CDS             20791..21939
                     /locus_tag="ACG8PO_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFQ0205983.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(21977..22390)
                     /locus_tag="ACG8PO_000633"
     CDS             complement(21977..22390)
                     /locus_tag="ACG8PO_000633"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HFQ0205984.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            22469..22900
                     /locus_tag="ACG8PO_000634"
     CDS             22469..22900
                     /locus_tag="ACG8PO_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HFQ0205985.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            complement(22999..23232)
                     /locus_tag="ACG8PO_000635"
     CDS             complement(22999..23232)
                     /locus_tag="ACG8PO_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFQ0205986.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            23415..25085
                     /locus_tag="ACG8PO_000636"
     CDS             23415..25085
                     /locus_tag="ACG8PO_000636"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HFQ0205987.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            25232..25618
                     /locus_tag="ACG8PO_000637"
     CDS             25232..25618
                     /locus_tag="ACG8PO_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0205988.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            25605..26303
                     /locus_tag="ACG8PO_000638"
     CDS             25605..26303
                     /locus_tag="ACG8PO_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HFQ0205989.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            26308..26772
                     /gene="lspA"
                     /locus_tag="ACG8PO_000639"
     CDS             26308..26772
                     /gene="lspA"
                     /locus_tag="ACG8PO_000639"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFQ0205990.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            26769..27680
                     /locus_tag="ACG8PO_000640"
     CDS             26769..27680
                     /locus_tag="ACG8PO_000640"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFQ0205991.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            27915..28454
                     /gene="pyrR"
                     /locus_tag="ACG8PO_000641"
     CDS             27915..28454
                     /gene="pyrR"
                     /locus_tag="ACG8PO_000641"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HFQ0205992.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            28468..29751
                     /locus_tag="ACG8PO_000642"
     CDS             28468..29751
                     /locus_tag="ACG8PO_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HFQ0205993.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            29764..30690
                     /locus_tag="ACG8PO_000643"
     CDS             29764..30690
                     /locus_tag="ACG8PO_000643"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HFQ0205994.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            30709..31989
                     /locus_tag="ACG8PO_000644"
     CDS             30709..31989
                     /locus_tag="ACG8PO_000644"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFQ0205995.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            31994..33070
                     /locus_tag="ACG8PO_000645"
     CDS             31994..33070
                     /locus_tag="ACG8PO_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase small subunit"
                     /protein_id="HFQ0205996.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            33075..36260
                     /gene="carB"
                     /locus_tag="ACG8PO_000646"
     CDS             33075..36260
                     /gene="carB"
                     /locus_tag="ACG8PO_000646"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFQ0205997.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            36314..37090
                     /locus_tag="ACG8PO_000647"
     CDS             36314..37090
                     /locus_tag="ACG8PO_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HFQ0205998.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            37087..38019
                     /locus_tag="ACG8PO_000648"
     CDS             37087..38019
                     /locus_tag="ACG8PO_000648"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HFQ0205999.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            38016..38732
                     /gene="pyrF"
                     /locus_tag="ACG8PO_000649"
     CDS             38016..38732
                     /gene="pyrF"
                     /locus_tag="ACG8PO_000649"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFQ0206000.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            38739..39368
                     /gene="pyrE"
                     /locus_tag="ACG8PO_000650"
     CDS             38739..39368
                     /gene="pyrE"
                     /locus_tag="ACG8PO_000650"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HFQ0206001.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQI"
     gene            39356..39670
                     /locus_tag="ACG8PO_000651"
     CDS             39356..39670
                     /locus_tag="ACG8PO_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0206002.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYL
                     MI"
     gene            39708..40349
                     /locus_tag="ACG8PO_000652"
     CDS             39708..40349
                     /locus_tag="ACG8PO_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbR-like 5'-deoxynucleotidase"
                     /protein_id="HFQ0206003.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            40392..41084
                     /locus_tag="ACG8PO_000653"
     CDS             40392..41084
                     /locus_tag="ACG8PO_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HFQ0206004.1"
                     /translation="MDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKGEETK
                     IEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSEHIIN
                     GKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVNPDVF
                     FPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPIQPLN
                     GREIYYFEEFEQ"
     gene            41081..41983
                     /locus_tag="ACG8PO_000654"
     CDS             41081..41983
                     /locus_tag="ACG8PO_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFQ0206005.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(42141..43847)
                     /gene="efbA"
                     /locus_tag="ACG8PO_000655"
     CDS             complement(42141..43847)
                     /gene="efbA"
                     /locus_tag="ACG8PO_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein EfbA"
                     /protein_id="HFQ0206006.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            44037..44660
                     /locus_tag="ACG8PO_000656"
     CDS             44037..44660
                     /locus_tag="ACG8PO_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0206007.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            45110..46159
                     /gene="trpX"
                     /locus_tag="ACG8PO_000657"
     CDS             45110..46159
                     /gene="trpX"
                     /locus_tag="ACG8PO_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan ABC transporter substrate-binding
                     protein"
                     /protein_id="HFQ0206008.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            46156..47037
                     /locus_tag="ACG8PO_000658"
     CDS             46156..47037
                     /locus_tag="ACG8PO_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0206009.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            47040..47810
                     /locus_tag="ACG8PO_000659"
     CDS             47040..47810
                     /locus_tag="ACG8PO_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0206010.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            48089..48943
                     /gene="aroE"
                     /locus_tag="ACG8PO_000660"
     CDS             48089..48943
                     /gene="aroE"
                     /locus_tag="ACG8PO_000660"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFQ0206011.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
BASE COUNT        15825 a         8596 c        10617 g        13994 t
ORIGIN      
        1 taaacataaa ctaatggatt tgggcggttt caggctcatc agaaaaatat agattctggt
       61 gaacaacaac cataaaaaag tgagaatcat tgcatgagag cttctttcca taaattttcc
      121 tttaagagta gtgcttaaaa gtacagaaaa aataataaat gcaaggatag gagcaattaa
      181 agtaaattta tttaataata aacaaaattt ttcagctttt ttattcttgg aataacgagg
      241 tattgttttc gataaaaaaa gatggtgcag acaaattatg ataattaagc atactatcag
      301 ataactcaaa atgaagcctt ctttcaaaaa attattcaaa accatatatt gtatttttta
      361 tttgataata acaatgggat tattaagaag tggcactaaa taaaagacta gtgtgtattt
      421 agccgattta attataaaca agtaaatgga taaagtatat gtaatgataa aagaaaaatg
      481 gattttctaa tttttaagct aaatgttaga tcacacatgt atatgctgga tagtcttata
      541 aaaaatcatg taatacagct tggtacatga tttttatttg tagctgtttt ttacatagac
      601 acaaaaaaga aaccacgctt gtagacgtgg tttctttttt actatctgtt tccagaagct
      661 gaggtgctgc tggatggtgt atggtcattt gcagcatccg ctccgctagt tgctggctca
      721 ctggaagaag caggaggagc agatgaagac ggtgtcgagc tttcttcgga tgaagaagaa
      781 ggaaccgttg ttgaagacgc tggctcgctt gtttctgctg tcgtgctttc ttttgaggac
      841 tcagcagttg attcgctttg actagtagag ctagagcttt gagtcgttgt ctctgttgtt
      901 gagcttccag gagtctggac atagctggaa gatggaatag ttgttgatgg tgtaattgca
      961 ttcgatctag ctgtatattg atctttgtag tacagttcac cgcccacacg aattaatccg
     1021 cttggcatct cccagtctgt atttgtcacg ttagctgaaa catactgcat aagctcgcga
     1081 taaacatcag aggctacgtg tgacgattct gaagtgacag gtgtcatttt tttattgtaa
     1141 cctgtccaca cggaaatcga ataatttggc gtatagcccg caaataggat atctggatac
     1201 accccagaag aaataccaag ttttgcatac tcgtcatctg tgtaattgga tgttccggtc
     1261 ttaccggctt gatataaccc agcgatttgc gcattcgtac cagtttcttc agtgatcgta
     1321 tcttttaaaa tgtctgtgat catataagct gtttctggag acatggctgt ttttccatct
     1381 ggctcataag tttcttctgt tccgtcttgg aagacgattt tattaacata ttgaggtttg
     1441 taataagtcc ctccattggc gaaagccgca taggctgctg ccatttttaa ggaagaagct
     1501 ccgtattttg tcccatcttg ttcttccgta ttactagaga tcgcattcga ttgatggatc
     1561 gtactgtatt cgatcccaag attttttaag aaagaagcca ctttatctga accgacttca
     1621 ttgaataatt tgactgctgg aacattacgg gataagtaaa gtgcttggcg taaagtaatg
     1681 gtgcccatat attgattatc ccagtttcct acaggagtcg aagtcccttc atagttatac
     1741 ggtgcgtcag taatagtttt tccagtagaa tatttcaaat attcaaaagc tggtccgtaa
     1801 tctgtgacag gtttcatcgt agaaccaaaa tcacgggaag tattcactgc taagttgttg
     1861 cctaaagtta cgtcttctgc gatgtgacgt ccgccaatct gggcttttac tttcccggta
     1921 ttggtgtcga taagggtgga ggcaacttgc atctcgtcgt ctgggtaaga gacatactga
     1981 tccgtgttga cgatatcata tagtttcttt tgggcgtcca gatccaaatt cgtataaatc
     2041 tctaaaccgt ctgtgtagac attcttatct gttttttctt gtacttcatt gatgacttct
     2101 ttgacataat tatcgacgat cttagtattg tcatcggatt gagttagttc ttgcagacca
     2161 tccgttaccg gtacattaac agcttgatca tattctgttt gagagatttt ttcgttttga
     2221 agcatcgtat acaaaacagt atctcgtctt ttcttcgcct gatctggata aacataagga
     2281 tcataggcgg aaggggcttg aggcatccca gcaagcaatg ctgtttgctg taaagaaagt
     2341 tctgataatt tcttaccaaa gtacatctca gaagctgtct ccatcccgta caagccgttt
     2401 gacatataca ctttatttac gtagtaggtc aagatctctt gtttcgattt tttttgttcc
     2461 aatcttacag ccatccacgc ttcttgggcc ttgcgtttca gagtctgatc ctcggcactg
     2521 gtggagaaga aggaaagttt gatcaattgc tgtgtcaaag tacttccgcc ttgcagtccg
     2581 ccagaggtaa agttagagag ggcagaaccg atgatccgaa tagggtcaac cccgatgtgt
     2641 ttataaaaac ggcgatcttc taccgacacg atcgcatctt ctaatgtttt cggtaattca
     2701 ttcgcagaaa ttttttcacg cttttctgcg cctaaatctt cgaatagttc gccatcttgt
     2761 gtatatagtt tggaagatac ggtagcgtct aattttttat ccgtcaattc gggcgcatct
     2821 tttgcgtaat accagaaaag tcctacacct gctaaaaaga ggatacagaa aaatgagagt
     2881 attcctaaaa ggatcttgat aaataatcct tttttatgtg ttccagagga ctttccagaa
     2941 cctttgccag aattcttctt cacactcttt ttaggagtag gctgtttctg tcgtctagag
     3001 cttctagttt gttcatttgc cattcttgct ttctccttca atagataatg ttgataaata
     3061 gaagtcaacc gcgtccaaat aaggaatccg cggagaaata ccagttttga tctctattcc
     3121 tagttcttcg atcaaagata aaggtagtga tttgcgtcct ttttcctttt gggcgtccca
     3181 gtaagcaatc aaatgaacac tattcaaata aaaacagcgc ttcaaagacg aaaaccataa
     3241 caagacaaag cagaccccat tttgttcgag gcatgctttc atatgttcga tttgatgggc
     3301 atggaagttt ttaaagggaa aagatgtttt gttcttggtt tcttttgctt caaagtccaa
     3361 gtaaaagcct cgatagacgc cattatagtc tgtagttgag gcttgggtaa agtaggcttc
     3421 cttgatcaca gcagcacttc tcttaggata gtctactttc acgatttgga taggggtagg
     3481 ctttttatgg ataaccgcca acccgcggga aagataatat ttattacttt cattgattgc
     3541 ttcttcaaag cgcatccccc gattgccgaa ttcagtgatc tgatttcttt tttcttgaat
     3601 tttttcgacg ggagaggcat tcttataata aggttggcca tttggatagc gcaataccat
     3661 ctttaatcac actcctaggc cacattatag caaaaaagcg gatagaaaga aaaggaatct
     3721 gagatggata agtcaaaagt aatctatgtg tctgggtatc gaagtttcga actcggtgtt
     3781 tttaaagaaa atgatcctaa aatccaagtt gttaaaaatg ttttaaaaaa agaactccat
     3841 cagttagcag aagaagggct ggaatgggta cttgtcagtg ggaatttggg cgtcgaacta
     3901 tgggctgtgc aaatagtagc ggaattgaaa gaagaatatc cggaactatc tcttggtatt
     3961 ttgtatcctt tttctgattt tggtaataac tggaatgaag ggaaccaaga aaaaaaacaa
     4021 ttagcagaac aattagcaga ttatgtcgaa gctgttagcc atcaacccta ccaatcacct
     4081 agtcagctaa agaatcacac tcgttttata cttgagcata cagtgggctg tctgctgatc
     4141 tatgatgaag aattccctgg aaaaacgaag tatttttttg aagatgcaca gaattaccaa
     4201 gcagatcatc cctacgaaat tcggctgatc agtatggacg atcttcaaaa ttttagtgaa
     4261 taatcgatat tgtgtttgat ttatcaactt ttttcctata caatgaaaaa ggtagaaatg
     4321 ttattacgta aatgaaaact agaggtgtaa atatggcgaa tttggtttat agtcctaaag
     4381 acattttaca acaagaattc aaaacaaaaa tgcgcggcta tgatcccgtt gaagttgatg
     4441 aattcttaga caatatcatc aaagattatg aaacttacag caaagaactc cttgctttac
     4501 aagaagaaaa cgatcgattg agtgcaaaag tagctcaatt atctaaaact caaggagcag
     4561 ctcaaacacg cgtgcaacaa acagaggcac caaagagcgc agcagtgacc aattttgata
     4621 tcttaaaacg cttgtcaaat cttgaacgtg aagtatttgg taaaaagctt gatcaacaag
     4681 caagtgcagt caaaccagca caacctaatc caaataacta cacaaatgca gacacaagct
     4741 tagatgataa tgaaaaaaca cgtcaatttt aatttttgat taatgaattt gcggttttcg
     4801 ggtaatcgcg gtctgacttg atcaggctga ggaaagtcca tgctcgcaca ggctgtgatg
     4861 cctgtagtgt tcgtgcttag cgaaaccata agctaaggta ctctttggag taacggcagg
     4921 aaaacaagct aaggcttcgg ctatgcttaa gtatccttga aagtgccaca gtgacgaagc
     4981 ggctggggaa acccatccgg tggaacgcgg taaacccctc gagcgagcaa cccaaacaat
     5041 ggtaggggcg ctttttgtct ggaagaagaa caggacaaaa aggcagtata actgcagata
     5101 gatggttacc gctttgtttt ttctccctgg aaaagcaaag tacagaacat ggcttataga
     5161 aaaccgcaaa catcagggaa ttaggtcgtg acaaaagtaa tgcgttataa gaaacaagaa
     5221 aaacatttga aagctatttt tcaatacttc agatgaatcg ttcttattac aaaacaaact
     5281 tttgtatcac cggttattcg gaatttgagt aaaagtgtgg ggtaggactg atgtcccacc
     5341 tccttttttt ataaataagg agattgtgta tggaaaaaaa caggaagttc aacctcgtcg
     5401 caactgcggc aagcgggcta gaagcactgg ttggtaaaga attaagagat cttggaatcg
     5461 aatgccaagt tgagaacgga cgtgcacgtt ttgaaggaac aatggaaacg atcgctacag
     5521 ctaatttatg gttacgtaca gcagatcgag taaaaatcgt ggttggggaa ttcgatgcgt
     5581 atacgtttga tgaattgttt gaaaacgtaa aagcattgcc ttgggaagat tttcttccac
     5641 tagatgcagc ttttcctgta gctggaaaat cgatcaagtc gaaactatat agcacacctg
     5701 attgccaagc gatcacaaaa aaagcaatcg ttgaacgtct ccgtacctat tatcaccgtc
     5761 cagcttctgt accactgacg gaaactggcg cacattttca gttggaagta gctttgctga
     5821 aagaccatgt gatggtaact ctggatacga caggaccaag tttattcaaa cgtggttatc
     5881 gcttagaaaa aggtggagca ccattgaaag aaaacatggc agcagcactt attatgttga
     5941 caaattggcg aaaagaccgt ccattttatg atcctgtatg tggttcaggt acgctttgta
     6001 ttgaagcagc cttaataggc cataatatcg caccaggatt caatcgtgag tttgcttgcg
     6061 aatcttggga ttggttcagt caagaaatca tggaaaatgt tcgatcagca gccgaagaaa
     6121 aagcagatta tgatattgaa ttagatatta ctggttcaga tatcaatgga cggatgattg
     6181 aaattgccaa agccaatgca gaagaaatcg gattaggctc ttcgattacc tttaagcaac
     6241 aagcagtcaa agacttcaaa acggataaag aatacggggt catcgtggct aatccgcctt
     6301 acggagaacg tctaggagaa gaagaaactg ttcggaaatt atataaagaa atgggcgatg
     6361 tattccgtcc attaaaaaca tggagtaaat atatcttgac tagcgactta gcgtttgagg
     6421 aattttacgg acaaaaagca accaaaaaaa gaaaattgta caatggtgca ttgcgtacgg
     6481 atctattcca gtattgggga actcgtccgc ctagaaaaaa agagaaagac gaataatctt
     6541 actaattgat tcattcaatt ggatgtttgg aggaataggg aatgaaagaa aaagagtttt
     6601 tacaagaagt aaaagaaatc aatcttctac aacaagcttt aggaatcctc gattgggata
     6661 cacaaacagg tatgcctgaa aaagctagtg cataccgaag tgaagtagac agctatcttt
     6721 acagcattta tttttcaaaa aaaatcggtc caaaaatcaa agaagccatt cgctattttt
     6781 ctgaacatcc ggaagaattg tcggaagttg gaactgccgt ttttgccaaa gtaaaagaag
     6841 agtatgattt ggaacatcgc gtaccggaag atcttatgag aaaatataca gctgccgttt
     6901 cttcagcaca cggacaatgg caaaaagcta gagaaacaca acaattttct gattttcaac
     6961 acgcattgaa tcaaaatatt gaattaacaa aacagttgat accttactgg agaaaagatg
     7021 aaaagacgaa ttatgatgtt ttgctgaatc aatacgaacc cgggatgact gtcgaaatcc
     7081 ttgataaggt atttgatcag cttaaagaag gaattctttc gatccgccgt acgctccaag
     7141 aaaaagggac tgaacctgaa acagactttt taaatcggag aatgacgaaa aaacaacaga
     7201 agagatttgt agtgaaagta atcgaacaac ttggttatga ttttagtcgt ggtcgtttag
     7261 atgacactgt ccacccattt atgactggaa ttaatccgaa tgatgcacga atcacgacac
     7321 gatggaacga aacagatttc aaaatggctg tttttggagt gatccacgag gctggtcatg
     7381 gtatgtatga acaagacatc gatgataaat atgcgtatac accggtttat gaaggtacat
     7441 caatgggtat ccatgaatca caatcgttgt ttaacgagat cattattgga agtagtcgca
     7501 gcttctggaa aaagcaatat cctttctttc aagaatgtgc agaaggaaca ttcgatgata
     7561 ttccttttga caaattttat cgttcattga accatacaaa atcaagtttg gttcgtatcg
     7621 aggcagacag tttgacttac cctctccata tcatcattcg ttatgaaatc gaaaaaatga
     7681 ttttcaatga aggggtagat gttgctgaac tacctgaaat atggaatcaa aaatatgaag
     7741 agtatctggg aatacgacca acaaatgatg tggaaggcat cctacaagac gttcattggt
     7801 caggtgcaag ctttggttat tttccctctt atgcattagg gtacatgtat gcggcacaat
     7861 tacttcattc tttgcagcaa gaaatagatg tggaaaatgt tctagcgtca gacgattatt
     7921 ctccaatcaa aaactggatg tcagagcata tccatcaata tggtgcatct agaaaatcaa
     7981 atcaattaat catggatgca acacatgaac cgcttaatcc gcaatacctg atcgactata
     8041 tgaaagacct gtattttaat gtatatcagg taaaataaaa tacaatttac aaagatagct
     8101 gaaattcatc taatctatag atgttttcag ctatttactt aagaaagaag tggaacgaat
     8161 ggataaatta caagtgatcg ctgattatag tatggaccaa ttagcgcatg atcaaacagg
     8221 acatgggagc gatcatacca aaagagttgt caagttagct gaaaaaattt tggagacgga
     8281 accgcaggca gaccgctttg tgacgcttgc agcagcttac ctacatgata cgattgatga
     8341 taaagtcgtt aaaaatgaga acgaggcaaa gcaacagctt cgtgtattcc ttcgctcttt
     8401 gccaatagta gaggaacaga tctcgatgat tttttccatt atcgaaaata tgtccttttc
     8461 taaaaatctc tctgaagcgg ccgaactatc tctggaaggt aagatcgttc aagacgctga
     8521 tcgtatcgaa gcattaggag caatcggcat tttgcgtact gcttatttcg gcggtggaca
     8581 tgggcacccg atttttgatc ctgaactgta tccgcaaaca ttcaaggaca aagaaaatta
     8641 ccgaaaaggc acgacagtga tcaatcattt ttatgaaaaa ctgttcttgc tgtctgataa
     8701 aatgaatact gattacggat atgaagaagc caaacgaaga gaaaccttca tgcgtgaatt
     8761 cttagaagaa ttttttctgg aatggtctat tgacgatgaa aattttactc cagacaattg
     8821 gatcaaaaat aacaaaaaat gatggaggtt gtgacagaag tggtcagctc caagatattt
     8881 cggatcattt tccgtaaaag ttacttctgg aacactgttt accaagaggt cgtgagcctg
     8941 ccgttcagct ccgagtcaca aggaacaatt tcaaaaaata gcttctcgtg tttgatgaat
     9001 attgtaactt gtgattcgga attggcagga gaacaccgtt tattcggaag aataggactg
     9061 tgacaagact tttgtcacag tccttttttc gaattcaaca agtaaaactt ttaagaaata
     9121 ttcagagagt aaaattaaaa attgctgata aacgaagatg gaaattttat gtatttttat
     9181 tggtctatgc aataaataaa ttaaatattc agaatacaat tgattttaac gaaagggctt
     9241 ctatcaatgg ttgtgttgta tttatctgcc caggtagagt aaaatagatc attatctaaa
     9301 aggtaattgt tcatactata taagtcattt gaaagtaaaa agatactgta aataaaaaat
     9361 gtggttaaat aattctcata atcagtgtaa gcgttatcaa aaatatagca tacttgcttt
     9421 gtagatgaat gacacgatat atgtttggta tgttctgcaa gacagatgaa tatggaggtg
     9481 gagacaatgt cttccaaaga actaacaatg tatttagaga aagaattgga tcaactgaaa
     9541 gaaaaggggt tatacaatac gatcgacata ctagaaagtg aaaatggtgc ttgtatcatc
     9601 gtagacggaa aaaagatgat caatctagca tccaacaatt atcttggatt tgccaatcgc
     9661 gaagaattga aaaaagcatg tattgaagca accgaaactt atggtgtagg agcaggagca
     9721 gtccgaacga tcaatggttc gctgaaaatc catcagcaat tagaagaaaa aatcgcagaa
     9781 ttcaaaggga cagaagcagc aatcgccttt caatctggat tcaactgcaa tatgggcgct
     9841 atctctgcag taatgacaaa agaagatgct atcttatcgg atgaattaaa ccatgcatct
     9901 attattgacg gttgccgctt atccggagca aaaatcatcc gaattaaaca tcaagatatg
     9961 aaagaccttg agaaaaaagc aaaggaagcg atagaaagta agaaatataa aaaaatcatg
    10021 tatatcacag atggtgtttt ctccatggat ggagacattg cacgattgcc ggaaattatt
    10081 ccgattgttg aaaaatatgg actgatcacc tacgtcgatg atgcgcacgg ctcaggcgta
    10141 actggaaaag gtgcaggaac cgtcaagcat tttggattaa gcgacaaaat cgatatgcaa
    10201 atgggcacgt tatcgaaagc tataggtgtt gttgggggtt atgtagctgg atcgaaaaca
    10261 ctaatcgact ggttgaaagc tagaagtcgg ccattcctat tttctacttc gctaactcca
    10321 ggtgcagctg cagctgcatt ggcttcgatt actttgatgc aagaacatcc ggaacttgtc
    10381 gaaaaagttt gggaaaatgc taattatttt aaagaagaat tgaaaaaagt aggctataac
    10441 atcggtatgt ctgaaacacc tattactcca gtcattttgg gagacgaaaa agtgactcag
    10501 actttctcta agaagctgat cgaacatggg atttatgcta aaccaatcgt ttatccaact
    10561 gtgcctcttg ggacaggcag gatcagaaat atgccgactg cggaacatac gagggaaatg
    10621 cttgatgaag cagtagcagt ttatcaaaaa ataggaaaag aaatggaaat tatttaacta
    10681 aatttagttt tttcgtctat ttaaaattag agaacatgtt cacgcatata ttgttaatca
    10741 tctgaagata gtagtaaaaa agatcaggga ggatcatcat gaaaaaagta ttggtgacag
    10801 gatgtttagg acaaattggt tcagagttga tcacacggct acgtcaagaa ctaggcagtg
    10861 gaaatgtcat tgcgacagat atacgcatgc ctgaagtcat tgagaaaaac gatatctttg
    10921 agatcctaga tgtaatggat tacgaaaaaa tgcgcagcat agcagaacaa tatcaagtgg
    10981 acacattgat tcatttggct gctcttttgt cagctgttgc agaagcaaaa ccacaattag
    11041 cctggaattt gaacgtgact gggctggtca atgcattgga agtcgctaga gaattccagc
    11101 tgaaattttt tacgccaagt tcaatcggcg cgtttggtcc aaatactcca aaagatcata
    11161 ccccacaaga tactcttcag cgtcctacaa ccatgtatgg ggtaacgaag gtagctggag
    11221 aactactatg tgattattat tatgtgaaat atggcgtaga tactcgtggt gtccgtttcc
    11281 ctggattgat ttcttatgca accttgccag gaggcggtac gacagactac gcagtcgata
    11341 tttattattc agcaatcaaa gaaggaaagt acactagttt cattgatcaa ggtacatcaa
    11401 tggatatgat gtatatgcca gatgcgatcg atgccattat aaaactcatg aatgcagatc
    11461 ctgatcgatt ggttcatcga aatgccttca atatatcggc gatgtctttt gacccggaag
    11521 agatcaaagc atctattcaa aaaatcatac cagattttga aatggattat gatgtggatc
    11581 ctgtacgtca gtcaatagca gaatcttggc caaattcact agatgtttct tgtgcgcaag
    11641 aggaatgggg cttttcgccg aaatacgact tagatgtgat gacaaaggat atgttggaaa
    11701 aactgaaaga aaaattggta gagacggtat aaaacaagaa ccacatccaa aaagtttttt
    11761 atacttttgg gattggctat ggtatgaaag gggacaaggg atggaagaat atcaagccac
    11821 gccagtaaaa gaagtaaaaa tagaaaatga attaaaacgt acgatgggat tttttactgc
    11881 tttatcgact gttatgggca cagtgatcgg tgcaggggta ttttttaaag ccgcaagcgt
    11941 agcagaagtt acaggatccg ccagtttgca tatgttttct tggtttctcg gtggtatgat
    12001 ttctgtctgt gcaggactaa caggagctga actggcagct gctattccag aaacaggagg
    12061 gatgattaaa tatatcgaac ggatttacgg aaatacagca gcttttttat tgggctgggc
    12121 gcaagtcgtg atttattttc cagcaaatgt agcagccttg tcgatcattt tcggtacaca
    12181 gttcgtcaat ctatttggtc tttcccagtc aatgattgtt cccgtagctg ttacagctgc
    12241 tgtatcgatc ctgttgatca attttttagg ttctaaagct ggaggggctt ttcagtcgat
    12301 tacgcttgtc tgcaagctta ttcctttgtt tgtgatcgtc attttcggtt tgttccgaca
    12361 agaaggagta gacttccaac tgtttccgat ccaagcagga gaaaatcttt catttttctc
    12421 agccttggga gcgggcttgc tagcaacgat gttcgcatac gacggttgga tacacgtagg
    12481 aaatatttcg ggagaattga aaaagccggc gaaagatttg ccaaaagcaa tttctttagg
    12541 aatcatcgga atcatgatcg tttatttatt agtaaatgcg gtgttcttaa gaaccgcatc
    12601 tatcgacgga gtcgcaggaa acagcaatgc ggcaagtgac gttgcaaaaa tgatttttgg
    12661 cggcttcggt ggaagactgg tcacagttgg tatcttgatt tctgtttatg gaaccatcaa
    12721 tggctatacc cttactggaa tgcgtctccc ttatgttatg gcaaaagaaa acaacctgcc
    12781 ttttagcaag ctctttgcta aacttcatga taaaactaaa gttcctgtag ccgcaggtat
    12841 cttagagcta gtcatcgcta ttggcatgat gatgatcggt ggttttgata cactaacgga
    12901 tatgctgatc tttgtcattt ggatttttta tacgatggtc tttgttggtg tcattcttct
    12961 tcgtaaaaaa gaacctgatt tgtttcgacc atacaaagtt ccaatgtatc cgtttattcc
    13021 actagtagca atcattggag ggacttttat cgtatcaagc acgcttatca ctcagacctt
    13081 tttagcttcg atggggatag tattgacact tgctggtatc ccgatttatc tctatttaaa
    13141 acgtagatag ctaaaacatt cgaattttat tagaactgca cttctctgcc aaacaaaata
    13201 ttttatttaa atgaggagta cgtggaagaa atgtcaaaag tactcgttaa ttttgatcac
    13261 gtaaatgtcg ttttgtctac gatgaaatag caaagtaaaa gacagaaatc gggctcaacc
    13321 gatttctgtc ttttactttg atttgcgtaa ctgaactatt cggcgtacgc cgcgataaat
    13381 atttaataaa accaagcaac cagaaatgaa aatgggaagc caaatcagat agacagggta
    13441 gccaagcact ttagacaaag catgtggagt cattttatcc atttcaagaa gtgcttgctg
    13501 ccaagtctga agattgaaga ctagcatgaa acccgaaata atcaggagaa taaatcctaa
    13561 ataatgaatt gtagttcttt ttcttacata agcgatcgta gcataaagga acagtaaaaa
    13621 aataggtacg ccaaaaacaa agtacatatt cagcatctcg acaccccctt tgtgtttatc
    13681 ttactttact ttgaaagaaa tgagaaataa tagcctcgtt acttgcttga aacagcaaag
    13741 gactgctaag ataagagaga tacaaagaat caaggaggag tcatatggca agttatatcg
    13801 aaccgattcg ttttgcgctg attgtttttc catttttagc cttagcgatc tcatctgttt
    13861 tttttattta tgaatatcga aagtatggaa catttctagt aacaagggca gttattttgt
    13921 attctttcgt tttttattta ttgtgtgcgt attttttggt gatcttacca cttccggcaa
    13981 aggagacggt tgcccaaatg acagggccaa agatggagct gcatttattt gcttcatggc
    14041 aacatttcag agacaacacg gtattggtct tgtctgaacc tagtacctac ctgccggcaa
    14101 tgaagcagag tgtatttttg gaaccagctt ttaatgtttt attactcata ccatttggca
    14161 tttatttaag atattatttc cggctttctt ttatgaaaac gatgattgct agtttctgcc
    14221 tttctctatt ttttgaattg acgcagctaa gtggacttta ttttatctac ccaagaccat
    14281 accgcttatt tgatgtgaat gatttagtga caaatacatt gggtggaatc attggttttg
    14341 tcgttacgcc gttatttaca tttatgcttc ctacaagaag acgaatggat gaactttctt
    14401 atgaaaaagg aaagactgtc tcattgactc gacgcttggt cgcttatttg atcgactgga
    14461 catttttatc tcttattaat ttggtagcgg cgattttctt taaaattgcg ttcgattcag
    14521 gggcagttac ttctcatatc tggtggcttt tattagaagt aggggtatac tttatgattg
    14581 taccgtttat aaccaatgga tggacacttg gcaaaaaaat tgtgcgtatc cgtttggtgg
    14641 aagaagaccg cgaacgaatt tctttaaaag cattgatgat ccgctacggg tatctgtact
    14701 ttctttttta tggtttaagc tggttggggc agttgcttca gcaaatcatg aattcgacaa
    14761 cgaatacggt gttgcagatg agtgcagtat tcatttctct cggtgtcttg ttactacaag
    14821 gtatttttat tttaaatatc ttgctatcat ttttgagaaa aaaacggatt ctgttttatg
    14881 aaaaagctag tcacacatat actgttagca cgattggcca aacaaaggat gatgattctc
    14941 gaaattagct gagcaaaaac gctttgtctg tgttataatg ccaaagtcgt gataatttaa
    15001 agagaagagg tgcacgaatg aataattttg atatattccg cttcaaaccg tttatcaatg
    15061 aagcgttaac cgcaaagggc ttcaaacagc ccacagaggt ccaggaacgt ttgattcctt
    15121 taattgcaaa aggaaaaaat atcattgggc aatctcaaac tggtacagga aaaacacata
    15181 cgtttttgtt gcctttgttt gaaaaaatca atgcagaaaa agaggaagta caagttgtta
    15241 ttacagctcc aagtagggaa ctagctgcac agatctatga agcagctaca caaatcgcta
    15301 aattttctac accggagatc cgtgtctcga attttgtcgg cggtacagac aaacagcgtc
    15361 aattaaatcg tctgcatcac caacagccac aggttgtagt aggcacgcct ggtagaatat
    15421 tagacatgat gaatgaacaa gcgctgaaga ttcacaccgc atttgcattt gttgtcgatg
    15481 aagcggatat gacattggat atgggttttt taaatgaagt agaccaaatt gcaagtcgat
    15541 taccagaaaa gctgcagttt ttagtttttt ctgccactat tccagaaaaa cttcgtccat
    15601 tcttacgaaa atacatggaa aatccaatca ttgaagaaat caaaccaaaa gcagttattt
    15661 ctgaagacat tgaaaattgg ctgatttcaa caaaaggtaa agatgagaat caaatgattt
    15721 atcaactgct gacaattggt catccttatt tagcaatcgt atttgccaat acaaaacagc
    15781 atgtggatga aattgccgat tacttgaaaa accaaggctt gaaagtagca aagattcatg
    15841 gagacatcac cccaagagag agaaaacgag tgatgcgaca agtacagaat ctggagtatc
    15901 aatatgttgt agcgactgat ttagctgcta gagggatcga tatcgaaggt gtctctcatg
    15961 ttattaatgc agaaattcca gaagatctag acttctttat ccatcgtgtc ggacgtactg
    16021 gacgtaatgg cttaaaagga acggccatta cgttatattc gccaaatgat gagcaagcca
    16081 ttgatgcgat agaaaaattg ggcgtctcat ttgaaccaaa agaaatcaaa aacggggaaa
    16141 tcgtcaaaac atacgatcgt aatagacgtg ccaaacgaga aaaatcaaaa gatgtattag
    16201 atcccacgct gattggtctt gtgaagaaga aaaagaaaaa gatcaagcct ggttataaga
    16261 aaaaaatcga ttgggcaatt gcagaaaaaa ataaaaaaga acgcaaaatc gaacgcagac
    16321 agcaaagccg tactgctcgt aaaaataaga aaaattcaca taaataaaaa acaagaggtt
    16381 gtgacaaaag gcgtgtcaca atctcttgtt tttaagctag actttttttc attccgatac
    16441 aaagtacttc atcttcaata aaaggttctg tgcaagtatg atacccttgt ttttcataaa
    16501 aatctacagc cgtcatttca gctactacat aactagtctg acaaccttct gaaaccgccc
    16561 gtttttcagc agcttcaagc aactgaccgc ctaacccttt tcctcggtat tcttttgcta
    16621 cgcaaaatcg ttccggcttg aagcaagtag gtgttttctt ttcataacgc aaagtagcta
    16681 ctggtctttt ttgatcaaga aatagataat aggtttgttg cgggatatct tttccgtcga
    16741 attctagttc tgcaggaatc ccttgttcta atacaaaaac ttgatatctc aaaaagcagc
    16801 tagctgcgcg tatccattca tccgctccaa ctattttttt catcatattc ctccttgtca
    16861 tgaatagcag agatgtattg attcctttat tcttatcgta ccaaaaatcg tggagtatga
    16921 ccaaaaggaa atgaaaataa aaattgagat attgacagtg atcaatagaa tgcgtatact
    16981 aaataagtac gaaaaggata tgatttgttt tttaagactc acagaaaatt ctttcttggc
    17041 tgaaaaaaga atagtcacta atcaaattgc tccctttttc tattttggta gaaataccga
    17101 cgcagctcag cgttatcgag tttaagaggt aatgattaca catcattgca atcaaggtgg
    17161 taccgcgagt tttcgtcctt ggctgtgatg gtgttttttt gtatttaatt tatagaaagc
    17221 aggtaatgac atgaaacaat taagtagtgc acaagttcga caaatgtttc ttgatttctt
    17281 ccaatctaaa gaacatacga ttgaacctag tgcgtctttg atccccgtga acgatccaac
    17341 cttattgtgg atcaactctg gtgttgcaac attgaaaaaa tattttgatg gttcagttgt
    17401 gccagaaaat ccaagaatca caaatgccca aaaatctatt cgaacaaatg atatcgaaaa
    17461 tgtcggtaaa acagcacggc accatacgat gtttgagatg ctgggtaatt tttcgattgg
    17521 tgactatttc aaaaatgaag ccatccactg ggcatgggaa tttttgactg atccaaaatg
    17581 gatggcattc gatccagaaa aactttatgt aacagtttat ccaaaagata cagaagcaaa
    17641 acgcatctgg catgaagaag ttggcctgcc actagatcac attattgatg tagaagacaa
    17701 cttctgggat atcggtgcag gcccatgtgg tccggatacg gaaattttct atgaccgtgg
    17761 agaagcttat aacgatttag cagaagacga tcctgaaaac tatccaggag gagaaaatga
    17821 acgttattta gaaatctgga acttagtatt ttcagaattc aaccataagc ctgataacac
    17881 atttgaacca cttcctcata aaaacattga tacggggatg ggattggaac gcatggtctc
    17941 aatcgttcaa gatgcgtcaa cgaactttga gacagacttg tttatgccta ttatccacgc
    18001 cgtagaaaag ctaagcaaac aagtaaaata tggtgaaaac ccagtaacag atatttctta
    18061 caaagtaatc gcagatcata ttcgcgccct ttcatttgct attggcgacg gtgcattgcc
    18121 atcgaatgaa gggagagggt atgtcttacg ccgtttgctt cgtcgtgctg tcatgcatgg
    18181 gaaaaaatta ggtattgaac aagcattctt atatcgttta gtccctgttg ttggggaaat
    18241 catggtaagt tattatcctg aagtacttca acaaaaagaa tttattgaaa aagttatccg
    18301 tacagaagaa gaacgtttca atgaaacaat caatgaaggt ttggaaatct taaatcaagt
    18361 gatccaagaa gtaaaaaacg aaaacgggac gactctagca ggaaaagaca tcttcaaact
    18421 atacgacaca tatggcttcc cggtagaatt aacggaagag gtggcagaag atgaaggact
    18481 gaaagttgat catgctggtt ttgaaaaaga aatggatgca caacgcgaac gtgctcgctc
    18541 tgcccgcagt actgaaaaat caatgggggt acaatctgct ttattgacag atatcaaagt
    18601 agaaagtaaa ttcattggat acacagaaca agaatcagat agtcaattat tggtcatcgt
    18661 taaaggtgag tctcttttga atgaagtcgc agaaggtgaa gctcaaatga tctttgatca
    18721 aacacctttc tatgcagaaa tgggtgggca agtagcggat caaggatgga tcatgaatga
    18781 agtaggcgaa atcgtagcag ttgttacaga tgttcaaaaa gcgcctaatg gtcaatttat
    18841 gcacaccgta gaagtaaaag caccaatgag tgaaggacaa acctatcatc tattgatcga
    18901 tcaatcgatg cgcaatcgga tcatcaaaaa ccatacagca acccatctac tgcacaaggc
    18961 attgaaagaa gtacttggcg agcatgcaaa tcaagctggt tctttagtcg ctcctggaca
    19021 tttacgattc gactttaccc actttggtca aatcacttca gatgaattag ctgaaatgga
    19081 aagaatcgtc aacgaaaaaa tctgggaagc tatcccagtt gaaacaattg aaacagatat
    19141 cgatacagcc aaaaacatgg gtgcaatggc tttgtttggt gaaaaatacg gacatgacgt
    19201 tcgagtagta aatattggcg gctggtcaat cgaactgtgc ggaggcacac atgtagcaaa
    19261 tacagaaaac atcggaatct tcaaaatcgt ttctgaatca ggaattggtg caggcgtgcg
    19321 acgaatcgag gctgtaacaa gtaaagaagc gtacgaacta cttgaagaag aagaaaaaca
    19381 actaaaacaa gtagcaacaa ttgttaaatc acctcaattg aaagaagttg tttcaaaagc
    19441 ggaacaattg caacagcaag taagagagct acaaaaggaa aatgaacaat tagctagcaa
    19501 gttagcgaat caacaagctg gtgatgtatt caaagatcca gaaacgatca atggaaaaac
    19561 aatcattgca gcacaagtaa aagtgaaaga tatgaatcag ctgcgtcaat tagctgacca
    19621 atggaaacaa aaaaatattt cagatattct tgttttagca aacgaacaag aaggaaaagt
    19681 cagtctgcta gcggcaatga cgaaagaatc aaatgaagct ggactaaaag ctggcgactt
    19741 gattaaagca atcgcaccga aagtaggcgg tggcggtggt ggacgtccag acatggcaca
    19801 agctggcggg aaaaatcctg ctggtattcc agatgcactt aaagctgcga aagaatgggt
    19861 tgcgcaacaa tagggcgttt acgtttgaca gaaaaacaaa agttcaaaca ttgaacgaaa
    19921 catgattatg ggggcgaaga caaacaagtc ttgccctcat acttttttca attggatggc
    19981 gcatctaatc aaatggtcgg attcggatgg tctttagttg acgaaagtga cagaaatttt
    20041 tacgattagt ataaataaaa aggggtgaaa aaatgaatag tagaaataag acctttcgac
    20101 tagtcttacg tgcgatcttg ttagcaatca tcatcgtaca agcgatggtt ccttggctgg
    20161 ggtttattcc attaggattc attagtttaa caattatcca tatcacagta attattgctg
    20221 cagtagtatt aggcccaaaa gatggcatgg tgattggctt gttttgggga atcgctacaa
    20281 ttgttcgtgc ctatgccatg ccaacaacgc catttgatac attagtgttc acgaatccga
    20341 ttatctcggt agttcccaga gtacttgttg gcttagtagc aggcttggtt ttccactgga
    20401 tttatcaaaa aaataaatca ataacgatca gctcagcctt tgcaggggta ctgggttcat
    20461 tggtcaatac agtcttagtg ttaggattca tgggattatt ttatacaggt gcaacggcag
    20521 atgcctatgg tgtagactct gctcttttat ttaaaacctt agcaggggtt gcagcaatca
    20581 atgggatacc agaagcaata ggtgcgggaa tcataacacc attactagca aaagcgttat
    20641 ttgcagcaac accgttgaaa ccagaataat gaccatcttt taatagtaat aggacgcacg
    20701 atagatagaa aaaggaagag aggagaggaa aacaatcgtc ctctccatct ttttacctga
    20761 gcaacaagca aactagaagg ggtaaaaaac ttggctaaga aacaagaaga aaaaaagaaa
    20821 caaataccgc aacagcggac aaaaaaagga gtactagcgg gtatcttagg attggttacg
    20881 aatctgatct tgtttgtctc taagctaatg atcgggcttt tttcaggaag tgtctccatt
    20941 atggctgatg cagtgaacag tctatcagat actgcttcat ccatattgac tttgatcgga
    21001 tttaaaattg ctgcaaagcc agcagatcag gaacatcctt atggacacga acgcttcgaa
    21061 tatatcagtg ggttatttgt ttcgatcatc attacttatg taggatttca atttttagag
    21121 tcttctgctg aaaaaatcat ccatccagaa aatgtctctt taacacccat cgtctttttt
    21181 gtcctaatca tttccatctt tttgaaattt gcacaaggca aaatgtatca ggttattgcg
    21241 caatccattg attctgaaac attgagagct acagcaacag atagttataa tgatgtcttt
    21301 actacactgg ctgtattgct ttcagcagca atcgaatggg taactggatg gcgtattgat
    21361 ggatatatag gttttgcttt agcattatat atcctttata gtggattgac gatgcttcga
    21421 aattttgtat acgaactatt aggaagtcgc ccaacgccag cagagattga agaaatggaa
    21481 gagcatttaa atgcttgcga tgatatactt ggctatcatg atttattggt gcataactat
    21541 ggacccaata aacgctttgc atcagtccac atcgaagtag atgaatcaat gaatttgaat
    21601 gtggctcata agattattga tctgatagaa aaagacttta aaaagtcttt ggatgtggaa
    21661 cttgtctgtc acttggatcc aatgcctgtt gataacgaaa aatactataa tatcttgcgg
    21721 cagttaaagc aaattcttct tgaaacaggt acagggctta atattcatga ttttagagta
    21781 attcatcaag gaaaaacgct gcaatttgat gtagtggtcc ctgagaaatt ccagtttgtt
    21841 gatacagaac tagaaagaat cttacgtagt caaatagaag aaaaaatcgg agattaccaa
    21901 ttggaaatta cgtttgatca caattatctt ttatattaaa tgagaaacta taaaaagact
    21961 ccttaaagtt tttaatttaa agagtctttt tttcttttaa ttgtttatgg agagcctgac
    22021 gtgcaagatt atctgctcct ttatttttgt tttcgggcaa ccattgcagg atcaataaag
    22081 aaaattcgga tagaagttct tggattcttt gaaggtaggg aaggaagagc tcgttcttcg
    22141 ttgcgttttt gtcgaccgta gaaactagtg ttttgctgtc tgaaaaacag aaaatcgttt
    22201 cattctgtaa gtcgtttttc tttagaaatt ctaaagtttt taaaaagact tcgaactctg
    22261 cttgatggtt tgtccctata gaaagcggga tagatagctg ttcttgttta ccttgataga
    22321 caatcaagat tccacctcca ctaggaccag ggttgccttt tgtagaggca tcaatgtata
    22381 cttttaacat aaaaagagat tctccttttt gttcaaacta atgttatggt aagattatat
    22441 cattactgaa tcggtgagag gttagagaat gaaaaaaaat attcgatggc agcctgaact
    22501 ggcacatacg atcatctatt ggtcattgac cttgatcatt ctgtttttta gtctgattct
    22561 ttctttagaa aacacaggtc cttattggaa aagcaatttt gttttaggta tttttcttgt
    22621 ttttctattt ctaggtttgc ggcgttcttt tattttaaaa tcaaactctg tgattatacg
    22681 ttatgctgct ttttggaaag accgagaaat atctgtcgga cagattcgat ctttgacgcc
    22741 aactaatact ggggtgcaaa tgatgttgca agatgagaaa gccccatctg ttttcatgat
    22801 gcgaaaaaaa gatcaagtag tcctttttga tcattttgaa aaaaacggac ccaaatcaga
    22861 atcaattcct tatccttata cgaaaaaaag aaaaagctaa ttttaaaaat cagaacctaa
    22921 aaaaacgaat ttttacagga aaagaaaaga gtgagacgta cggttcatcc cgtttagcgt
    22981 ctcactctat ttgtttatct attcatgcat actagtatcg gtagtctgaa cgttgccttt
    23041 cttgacatgg gcagcttgtg ttcctcgatt cccttcgatg atctcaaatt cgaccacttg
    23101 attttcttcc aaagttttga atccctcttc ttcaatcgca gtaaaatgta caaaaatttc
    23161 ttctgtgtca tcataactga taaagccgta tccttttttg ttgtcaaacc attttacaat
    23221 tcctgtggtc aaagcactca cgacctttct aaagtctgat catagccttc attataaaga
    23281 gtatttataa gtacgtcaaa taaattaaaa acctgtaatt ttatgttttt tttctgaata
    23341 aaaaaaggta taattggcat atcttaacaa agaagatatt ttgttgaata aagttcgtga
    23401 aaaggggtaa tcaaatgaag aatgatctgc aaatttcaca agaaacagag ctagaaccga
    23461 ttgttgaagt ggctagtaaa ttagatctta aggaagacga tttggaactg tatgggaaat
    23521 ataaggcgaa aatcaatttt tctggaatca atcgatcaaa ggataacgct gaggggcatt
    23581 tgatcttagt tacttccatc aatccaacac cagctggcga aggaaagtca acgatcactg
    23641 tagggttagg agacgcgctc aataaaatca ataaaaaatc tgttattgca ttgcgagaac
    23701 cctcacttgg tcctgtgatg ggaatcaaag gtggtgctgc aggcggagga tatgcacagg
    23761 tattgcctat ggaagatatc aacttgcatt ttactggtga catgcatgct atcacaacag
    23821 ctaataatgc attatctgct cttttagata atcatattca tcaaggaaat gaattgaaca
    23881 tcgatgcccg ccgagtgatt tggaaacggg tagtcgatct caatgatcgt gaactgcgta
    23941 aagtaattgt tggcttgggt gggccaatcc aaggtgttcc aagagaagat gggtttgata
    24001 tcacagttgc cagcgaaatc atggctatct tgtgtcttgc ggcagacctt gaagatttaa
    24061 aggctcgtct tgcaaggatc gttgtcggct atacatttga ccgtcagcct gtgactgctg
    24121 gagacttgaa agcagaaggc gctctggcat tactgctgaa agatgcaatc aagccgaatc
    24181 ttgtacaaac aatttacgga acaccagctt ttgtccacgg gggaccgttt gccaatatcg
    24241 ctcatggctg caatagtatt ttagctacca agacagcatt gagattagca gattacgttg
    24301 tcacagaggc aggatttggt gcagatcttg gcggagagaa atttttagat atcaaagtac
    24361 ctaacttgaa aaaagcgccg gatgctgtgg tgattgtcgc cactgtccgt gcattgaaaa
    24421 tgcatggcgg aatgaaaaaa gatgaactta aaaatgagaa tcttgatgct ttgaaagtag
    24481 gttttgcaaa tttaaagcgc catatacgta atatggaaca atatcagcta cctgtgatcg
    24541 tcgcaatcaa tgaattcgta acagatacag acaatgaact cacactgttg gagcacttgt
    24601 gtgaaaatca ggggattctt gccaagagag cgagtgtatg ggcagatgga gcagaaggcg
    24661 gcgttgattt agcaaaagct gtagtacgat tgattgatag aaaagaagct aattacaagc
    24721 cattataccg cctagaagaa acgatacaag aaaagacaga aacaatcgtc aagaaaattt
    24781 acggaggaaa tggtgttgtt ttcagcaaaa aagcgcaaaa acaaatagaa gagtttacga
    24841 agaatggatg ggacaaactg ccgatttgta tggcaaaaac acagtattca ttttctgatg
    24901 atccttcact gctaggtgca ccggaagatt tcaccatcac gatccgcgaa atcatcccaa
    24961 aattaggggc aggattttta gtagccttga ctggagatgt tatgacgatg cctggtttgc
    25021 ctaaaaaacc tgctgcattg aatatggacg tgacaaatga cggagaagtg ttgggattgt
    25081 tttaagcttg attataatct accatcgaag gagctgtatt ttgactacag cttctttttt
    25141 tagacgatag ggaattaatt ttgtttacac taggatcatt ctttttcttt attgtatttt
    25201 gagctatact aaagcataat aggagggtat gatgaaaacg acatttataa gaaaaaccgg
    25261 tttttacggt atgggtagct cgattcagct aagaatcaac aatgaaaaac gcagtgttct
    25321 cagtcatgaa caaaagataa cgcttgatct ggaagtgccc tttacgatgc aggtcacctt
    25381 ttattggcta aaaagccccg tttatacagt gaaagaagca aagcctgctt atgtgatcac
    25441 aatgaattac ttgattcttc aaatctatcc gtttttattt ctagcaactg ggttgggggc
    25501 cgttttccta caaaatttat ttttcagttt ttttgcagtt attgtgttga ttgcgttctt
    25561 tttctatata aaaaaccggg catatttgat caaggaggaa aattatggca aactctgatc
    25621 tttttttaat cagtttcaat cgaatcgaaa aatggatgag agacgagatg ggaaatgcac
    25681 gaaatatggg atttaccgaa cttgtcagac gcctagctca aagaaaacaa ttaatgatca
    25741 gaaaatacga agatgatctt ttacagcttg ctcagttaag gaatgcgatc gtccatgatc
    25801 ggatcgcagt cgattttgtc attgcagagc caaatgattg gacagtcaag cgaatccagc
    25861 tgatcgaaca agaactgatt cagcctgaaa ccgtgctgcc tcgatttgca aaacgtgtaa
    25921 caggttttga acaagatctt ccgttacttg aactgttaaa gatagtagca gaaaaaaggt
    25981 attcacaatt tccgttatac aataaaggaa aatttgtagc attgattact ttacgtaata
    26041 tcgggttttg gctagcaaaa gaaagccaaa aaggtccggt tgatttaaca aataaacgag
    26101 cgttggattt gatcatccaa aacggaaagt acacaaatta tcattttgtt ccagctaaaa
    26161 cacatattta cgaggttgaa gcgatgtttc gtgagcaagg gactttggaa gccgtactta
    26221 tcactaaaga tgggaatccc gatggtaatc tgctcgggat cgtacgacct cgtgatattt
    26281 ataaacaaat agaaaaggat tgattttttg ttaattattt attggatatt cagcgctttg
    26341 ctagtaggag tggatcagtt ggtcaaatgg tggatcactg ataatctagc gcttggagag
    26401 acgaaaaatc ttataccagg tattttgtca ctgaatcata ttcgaaacac tggagccgca
    26461 tggagtatgc tagaagggaa gatgtggttc tttactatag tgacactcat tgcagtcgtt
    26521 gtgattctca cattgatgat taaaaatcgt gaaaaaggta atcgctggtt catgattgga
    26581 ttgagtttga ttctagctgg tgcgattggc aattttatcg accgtattcg tcttggctat
    26641 gtagtagata tgttccagac agattttatg agctttccga tttttaacgt agcagatgtg
    26701 acacttgtat gtggagtgat ttgtatgtta atttatatta ttttagatga aaaggatcaa
    26761 agaaaaaaat gacagaactt aaaacgacga ttcaccatga atctggtagg atcgataaag
    26821 tattgactaa tttattcacc gattacagtc gttcgcaaat tcagctgtgg ctgaaaaatg
    26881 gagcggtttc tgtcaatgga cagccagtca aagcaaatta caaagtaaaa caaggagacg
    26941 agattactgt cttgatcccc gaaccagaaa cattagatgt ggtcccagaa aatatcccgt
    27001 tgacgatcgt ttacgaagat gaagatgtag cggtgataaa caaacctcaa ggaatggtcg
    27061 ttcatccttc agcaggtcat acgcatggca caatggtcaa tgcgctgatg taccatatca
    27121 aggatctttc tacgatcaac ggcgttattc gtccaggtat tgttcaccga atcgacaaag
    27181 atacttccgg actgctaatg gttgccaaaa atgaccaggc acacgaatca ttagctcagc
    27241 aattaaaaga taagacctct ttgcgcaaat atgttgcttt agtccatgga gagattcctc
    27301 acgaaaaagg aaccatcaat gcgccaatcg gccgttcgaa ggaaaaccgg aaaatgcagg
    27361 cgataagaga agatgggaag tcggctgtca cacattttac tgtattggaa cgtttcgaag
    27421 gatttactct ggttgaatta aaactagaaa ccggaagaac acaccagatc cgtgtgcata
    27481 tgaagtatat cggttatccg ttagctggag atccagtata cggaccgaag aaaactttga
    27541 aaggcaatgg tcagtttctc catgcgcaaa tgttaggttt tacgcacccg actacaaacg
    27601 aaaagatgat ctttgaagca cctcttccag aagtttttga aaaaacttta gaaaaattaa
    27661 gaaaaaacat tgcattttaa tttagataac ggtatagtaa ttaacaacga aagcataaaa
    27721 cttaatagaa ttcctttaag tttagtcccg tgaggctaag aagggtggga gcaaggatac
    27781 gtatggtgcg ccataagtag attgctcatt gacatagtat agctatcagt gtatcagtat
    27841 acgctcagtc taaacattcc tcctgtcctc ttagggcagg agttttttta tgcaaaaaag
    27901 tagggggaat gaaaatgcaa gcaaaagaag tagtagatca ggtaaccatg aagcgagcat
    27961 tgactcgaat tacttatgag attatcgaga gaaaccatag tatccaagat atcgtattgg
    28021 taggtatcaa aacacgagga atctacattg catcaagaat tgccgaacgc ctgaagcagc
    28081 tagaagatat cgatattcct gtaggagaac tggatatcac tttataccga gatgacaaaa
    28141 aagaaaatcc agaagaaccg gaacttcatt cttctgacat cccagtttcc ttagaaggaa
    28201 aagaagttat tttgattgat gatgttttgt atacaggacg aacgattcga gcagcaatgg
    28261 acgcagtaat ggattttggc agaccaagaa aaatttcatt agcagtcctg gtcgatcgtg
    28321 ggcatcgaga attgccaatc cgtgcagatt atgttgggaa gaacattcca acatctcggg
    28381 cggaagagat tttagtagag atgcaagagc ttgatggaca agatcgcatc atgattttga
    28441 aagaggaaga ttagaggaga gagaaacatg tcggagaaaa aaggatttca taatgatgac
    28501 gtggtgttag atatccacga tcgcccaacc ccaggacatt gggtaggatt aagtttacaa
    28561 cacttgttca cgatgttcgg agcaaccgtg ttagtcccca tcttagtagg tatcgatcca
    28621 ggtattgcgc tagtcagttc aggactaggt acattggtgt acttatttac tactaaagga
    28681 aagatacctg cgtacttagg aagcagcttc gctttcattg cagcgatgca gcttttgatg
    28741 aaaacagatg gttatccagc gattgcacaa ggagcagtca caactggatt agtctatttg
    28801 attgttgcgc tgatcatcaa aaagatcggt tcagcatggc tggataaaat cttgcctcca
    28861 atcgtcgtcg gtccagtcat catggttatc gggttgggac ttgcggcaaa tgcagcaaca
    28921 aatgctatgt ataatcaggg cacgtatgat ttcaagtata ttgctgtagc gctgatcaca
    28981 ttaggattaa caattttctt caacatgtgg ctaagaggtt ttctcggatt gattccgatt
    29041 ttactaggta ttattagcgg atacatcgta gccttgctgt ttggaatcgt ggatactaag
    29101 ccaatcgtag acgctgcctg gtttgctgtt cctaactttg agattccctt tgtacaatat
    29161 gaaccaaagc tatatcttgg agcaatcaca acaatggcac ccatcgcttt tgtgacgatg
    29221 acagagcata tcggccatct tatggtattg aataaactaa ctaaacgtaa tttctttgaa
    29281 gaaccgggtc ttcataagac gttgacaggt gatggattag cacaaatcgt ggcagccttt
    29341 gtcggaggtc ctcctgtaac aagttacggt gaaaacattg gtgttctggc tatcacacgt
    29401 gtccatagcg tgtttgtgat cggtggagct gcagtatttg cagtcatttt aggattcgta
    29461 ggaaaattaa gcgctttgat tttaagtatc cctggaccag tcatctcagg aatcagtttt
    29521 atcttatttg gagtaattgc cgcaagtgga atgaagattt taatcgagaa caaaatcgat
    29581 tttgataaaa agaaaaatct gttgattgct tcagttatct tagtagtagg aattggcgga
    29641 ctcgtctttg aagtaggcac atttacattg tcggcaatgg cattagcaac tgtacttggc
    29701 atcattttaa atcttatctt accagaaact tcacgaagcg aagaaaacta aggaggaaac
    29761 attatgatca tcaaatcaga acgtatcagc ttaaagcatt tattgaccgt tgaggcactt
    29821 actgatcaag aagttatggg cttgatccgc agagggcagg aattcaagca aggtgctaat
    29881 tggactccgc aaaagcgcca gtactttgct acaaacctct ttttcgaaaa tagtactcgc
    29941 acacataaaa gcttcgatgt agcggagaaa aaactagggc tagaagtgat tgaatttgaa
    30001 gcaagcacaa gctctgtcca aaaaggggaa acactatacg atacagtact gacgatgtca
    30061 gcattaggcg tagacgtagc ggttatccgt catggtgatg agaactatta tgacgaactg
    30121 atacaaagta aaacaatcca gtgttcgatt atcaatggag gagacggaag cggacagcat
    30181 ccaacccaat gcttgcttga tttgatgacc atttatgaag aatttggtca ttttgaaggt
    30241 ctgaaagttg ccatcgtagg tgatatcact cactctcgcg tagctaaatc caatatgcag
    30301 atgttaaaac gtctaggcgc acaagtattc ttctcaggtc ctagagaatg gtacgacgat
    30361 gaattcgaag tgtacggaca ttatatgcca ttggatgaac tactagatca agttgatgtc
    30421 atgatgatgc ttcgggtgca acacgaacgc catgatggaa aagaaagttt ctcaaaagaa
    30481 ggctaccatc aggaacatgg gttaacagtt gagcgtgcga agaagttgca aaaacatgca
    30541 atcattatgc acccagcacc agttaaccgt gatgtagaat tagctgattc attagtggaa
    30601 ggattacagt cgcggatcgt tcagcaaatg agcaatggag tattcgtcag aatggcaatc
    30661 ttagaagcag tgctaaatgg aaaagcgtaa ttataggagg atgaaaaaat gaaaacactc
    30721 attaaaaacg gtcagatcaa tacacgaaaa aatatgacga caccagcaga aatttggata
    30781 gaagatgggc gaatcaaagc aatcggtaca ggattctcag aagccgaatt tgatgaagtg
    30841 tttgatgcaa agggacaatt gatcacacct ggtttagtgg acgtccatgt tcatttgaga
    30901 gagccaggat ttacatacaa ggagacgatc gaagctggga ctcgctcagc tgctcgtggc
    30961 ggtttcacta ctgtatgcgc gatgccgaat ttaaatcctg tacctgatac ggcagaaaag
    31021 ctgagacaag tatatgacat cattagaaaa gatgcagttg tgaaggtatt gcagtatgcg
    31081 ccaatcacag aaaatctgcg aagtgagaaa ttagttgatc aagaagcact gattgaagaa
    31141 ggtgcttttg ctttcacaaa tgacggtgta ggtgtccaaa cagctgggac gatgtatctg
    31201 gctatgaaag aagctgcaaa aaacaataaa gccctagtag cccatacaga agatgaatct
    31261 ttattatttg gcggcgttat gcacgctggc aagaaggcag aagaacttgg tcttcccggg
    31321 atcttaagcg taaccgagtc ttctcaaatt gctagagatc tgttgctagc tgaagcaact
    31381 ggtgttcatt accatgtttg ccatgtttct actaaagaaa gtgtccgtgt aatcagagac
    31441 gcaaaaaaag caggaatcca cgtaacagca gaagtttcac cccatcatct tattttgatc
    31501 gacgaagata ttcccgaaga tttcggcttt tggaaaatga acccgccgct aagaggacgg
    31561 gaggacagag aagctttgat cgaaggcttg cttgacggta cgatcgattg catcgctaca
    31621 gaccatgccc cccatggatt agaagaaaaa agccaaagtt tcatgaaatc accatttggt
    31681 atcgtaggaa gcgaaacagc gtttcaactg atctatactc attttgtaga aactggtcga
    31741 ttcacgttgg aacaagtgat caattggttg gcagttaaac cggctgagat ttttggtttg
    31801 aatgcaggaa cgctaacggt tggtgccccc gctgatgtag cagtttttga tattacacaa
    31861 acttgcacga tcgataaaga agacttttta tcaaaagggg agaatacacc ttttatcggc
    31921 tggaaagtaa aaggcgaaac acaaatgact tttgtcaacg gaaaacttgt gtggcagaag
    31981 ggagaataaa agtatggaac gtttgcttat attagaagat ggaacagttt tcgaaggaaa
    32041 ggcttttggt gcagatgcta gtgtcttcgg agaaatcgtt tttacaacaa gtatgactgg
    32101 ttatcaagaa actatcactg atcaaagttt taacggtcaa atcattacat ttacctatcc
    32161 tatggtgggt aattatgggg taaatagaga tgattatgaa tcgatttctc caacatgcaa
    32221 aggagtagta gtaaaagaac atgcacgtgt agcttcaaac tggcgaaacc aaatgacgct
    32281 agattcattt ttgaaacaaa aaggaattcc tggaatatca ggtatcgata cgcgcgccct
    32341 tactcgcaaa atccgtgaag tcggaacgat gaaagctagt atagttgatg ctggagattc
    32401 cttcgaacat gcatttgatc agctgaaagc aaccgttcta gcaaccaatc aagtgcagca
    32461 agtctcaact aacaaacctt atccaagtcc aggaacaggt cggaaagtca ttgtcattga
    32521 cttcggcttg aaacatagta ttttacgaga attgagtaaa agaaactgta acctcacagt
    32581 acttccctat aacacagatg ccgagacgat tttagcgcta tcaccagacg gagtcatgct
    32641 gacgaatgga cctggtgatc caaaagatgt gccagaagca atcgaaatga tcaaaaaaat
    32701 ccaaggaaaa gtaccgattt ttggtatttg tctcggtcac cagttatttg ctttagccaa
    32761 cggtgcagat acctataaaa tgaaattcgg acatcgagga ttaaatcacc cggtacgaga
    32821 agtagcgacc ggaagaattg attttacttc tcaaaatcat ggctatgcag tcgatgaagc
    32881 cacgattgat ccagaaaaac tcatcgtaac tcatgtagaa gtaaatgacg gaacagtaga
    32941 aggagtcaaa cacagagatt atcccgcttt cagtgtgcag tttcatccag atgctgcccc
    33001 tggtccgcat gatgcactac atttatttga tgaatttatg gaaatgatgg acgcaggaaa
    33061 ggagcagtaa acagatgccg aaacgtacag atatcaagaa aattatggtg attggatctg
    33121 gtccgatcat cattggtcaa gccgcagaat ttgactacgc aggaacacaa gcctgccttg
    33181 ccttaaaaga agaaggatat gaagtggttt tagtaaattc taatcctgcg acgatcatga
    33241 cggataaaga agtggcagat caagtatata tcgaaccgat cacacttgaa tttgtatcaa
    33301 ggattcttcg aaaagagcgt ccagatgctc tccttccaac tttaggcgga cagacaggcc
    33361 tcaatatggc aatggagctg gcagcatcag gtattttaga agaattgaac atagaacttc
    33421 taggaacaaa gctttctgcg atcgatcaag cagaagatcg tgatctattc aaacagttga
    33481 tgaaagaatt gaatcagcca atccccgagt ctgaaatcgt caatacagta gaagaagctg
    33541 tagattttgc aaataaaatc ggctatcctg tcatcgttcg tccagccttt actttaggcg
    33601 gaactggtgg aggtatgtgt gaaaatgaag aagaattacg tatcgtatca gaaaatggat
    33661 taagtctttc ccccgctacc caatgtttga ttgaacgaag tattgcaggt tttaaagaaa
    33721 ttgaatatga agtgatgaga gattcggctg ataatgcaat cgttgtctgc aatatggaaa
    33781 attttgaccc ggtgggtatc catacaggtg attctatcgt atttgcccct agtcaaacat
    33841 tgtctgacca tgaatatcaa atgcttcgtg atgcttctct ttcgattatt cgagcactta
    33901 agatcgaggg aggctgtaat gtccaactcg ctcttgatcc acatagtttt aattattatg
    33961 tgattgaagt caatccgcgt gtttctcgtt catctgcgtt agcaagtaag gcgactggct
    34021 atccgatcgc taagctagct gctaaaatag cgcttggatt aacattagac gaaatgaaaa
    34081 atcctgttac aggaacgact tttgcagaat ttgaacctgc attagattat gttgtagcta
    34141 aaattcctcg ctggccgttt gataagtttg aaaacggaga acggctttta ggcacgcaga
    34201 tgaaagcaac tggtgaagtg atggctatcg gtcggaatat cgaagagtct ttattgaaag
    34261 ctgtgcgttc tttagaaatc ggtacacacc atatcgaact tcctgagttg gcagacatca
    34321 cggacgatga cctaatccaa aaagtggttc gagcacaaga tgatcgcctg ttttatgtag
    34381 ctgaagctat tagaagaggg tacaccatag aagaattagc ggatttgaca aaaatcgatt
    34441 tattcttttt agataaactg ttgcatatcg tagaaatcga acagcagttg aaacagcagc
    34501 cgttggatga caagatattg aaaacagcga aacaaaacgg ttttacagac aaaaaagtgg
    34561 cggagctgtg ggaaataaac gaagaagccg ttcgccagat gagaaaagaa aatgatttga
    34621 ttcctgtata taagatggtt gacacttgtg cagcggaatt cgaatcaaaa acaccttatt
    34681 tttatagttc ctatgaatat gaaaatgaaa gttcacgatc agaaaagccc tcaattctag
    34741 tactgggatc tggtcctatc cgtatcggac aaggggttga atttgactat gcgactgtgc
    34801 attctgtcaa agcaatccag caggcaggat acgaagcaat catcatgaac agcaatcctg
    34861 aaacagtttc gactgacttt tctatttcgg ataaattata ttttgagcca ttgaccttcg
    34921 aagatgtgat gaatgtgatc gagctagaac agccgatagg agtaatcgtt caatttggtg
    34981 gtcagacagc aatcaactta gccgaacctt tgactgctgc tggtgtaaaa atcttaggga
    35041 caacgatcga agacttggat cgtgcggaaa atcgtgattt attcgagcaa gcgctgaaag
    35101 agttggatat tcctcaacct ttaggggata ctgctacaag taaagaagaa gccgtggtta
    35161 tcgcttcgag aattggttat ccagttcttg ttcgtccaag ctatgtatta ggcggacgtg
    35221 cgatggaaat cgttgaaaac cagcgagatt tagaagatta catggaacat gctgtgaaag
    35281 catctccaga acatccagta ctagtggacc gttacttgct tggtggtgaa tgcgaagtgg
    35341 atgcgatttg tgacggtgaa acagtactca tcccagggat catggagcat attgagcgtg
    35401 ctggtgtcca ttcaggggat tcaatggctg tgtatccacc tcaaacatta tctgaatcga
    35461 tcaagaaaac gatcgaagat tacacgatcc gcttagcatt aggtttgaat tgtgtcggga
    35521 tgatgaatat ccagtttgtc atccatgaaa atgaggttta tgtgatcgaa gtcaatcctc
    35581 gagcaagtcg gactgttcca ttcttaagta aggtgacagg aattccaatg gcacaaattg
    35641 cgacgaaagc tattttggga gagaaactaa agacacttgg ttatgaaaac ggactttatc
    35701 cagaaacaaa agaagttcat gttaaagcac ctgtcttttc ttttacgaaa ttgcagaaag
    35761 ttgatactta cttagggcct gaaatgaaat cgactggaga agtaatggga tctgacagac
    35821 aattagacaa agctttgtac aaggcattcg aagcttctgg attacacttg cctgaatatg
    35881 gagccgtatt atttacgatt gcggacgaaa caaaagaaga agcactagaa ttagctaaac
    35941 gttttacaga aattggctat agtctgatag gaacgaaaca tacagctgct tatttcgaaa
    36001 aaaatggtct ggtcgttgaa acagtagcga aaatttctga ggaagcagca gaaaaaaatg
    36061 tagtcgactt aataagagat ggtaaaacac aagtggttgt caatacgatt gataaagatc
    36121 ggggaaatgc ttctaaagac ggtttcatca tccgaagaga agcagttgaa catggtattc
    36181 cattgtttac ttctttagac acagcagatg cgatcgttcg agtgatggaa tcccgtgctt
    36241 tttcaactaa ccctatttaa cggcgagcac atgttaagac aaaaagacga aaaaccgttg
    36301 caggagggga cagatgaaac aagaaatgat ggaaatcgtt cagcaaaaac agctggcacc
    36361 aaagattttt gaaatgacat tgaaaggtga tctagtaaaa gaaatgcagc aaccaggaca
    36421 gttcttgcat attctcgtgc cgcgtgcaga cttgctatta cgccgaccga ttagcttgaa
    36481 tcatatcgac cacgaaacaa atacttgccg tatcatttat cgggtggaag gtgaggggac
    36541 tagagtcttc tctactcttt ctgccggaga ccatttagat gtcatgggtc ctttaggcaa
    36601 tggttttgat ttgtccaatc ttcatacagg agacgaagta tttataatcg gtggaggaat
    36661 cggtgtccct cctctatatg agttatcaaa acagttagtc gaaaaaggaa ttaagccaac
    36721 gcatttttta ggttttgcca cacatgaagt cgtttattat gaaaaagaat ttaatcgtct
    36781 aggagaaaca agaatcgcta ctgacgatgg aacatacggt atgcacggaa atgtcggtaa
    36841 tttattgtta ggaacgaaga cggaacctac agctgttttt gcatgtggtt caaacgggtt
    36901 attgaagaca gtggaacagc tgttttcttc gcatcaaaat gtccagttat cattagaatc
    36961 ccgcatggca tgtgggatag gggcttgcta tgcttgcgtt tgtcatacgc cggaagatga
    37021 aacaggaaca aaaagtgtga aagtttgtga cgaaggaccg atttttaaaa taggagaggt
    37081 aattatatga atgagaatcc tttagcggtc aaacttcctg gcctagaatt aaaaaatcct
    37141 atcatgccgg caagcggctg tttcggtttt ggcaaagaat acggaaaata ctatgatttg
    37201 aatcagcttg gatcgatcat ggtcaaggca acgactccaa atgcccgctt tggcaatccc
    37261 acgccaagag tggcggaaac accatctggc atgttgaatg caattggttt gcaaaatcct
    37321 ggtctagacg ttgttatgca taaacttctt ccagaattag aggagtacaa tgagttaccc
    37381 atcatcgcaa atgttgctgg tgcttgtgaa gaagactatg tagaagtttg tagcaaaatt
    37441 ggcgaagcac caaatgtgaa agcaatcgag ctgaacattt cttgcccaaa tgtcaaacat
    37501 ggcggaattg cttttggaac agattctgat gttgcttttc aattaacaca agcagtaaag
    37561 aaagtttctt ctgttcctgt ctatgtcaaa ttgtccccaa atgtaacaga catcgttcca
    37621 atcgctcagg caatcgaagc tggaggggca gatgggttta cgatgatcaa tactcttctt
    37681 ggtatgcgga ttgaccttaa gacaagaaaa ccgattttag ccaatcaaac aggtggactc
    37741 tccggaccag caatcaagcc tgtcgctatt cgtttgattc accaagtagc aagtatatcc
    37801 agccttccaa tcattgggat gggcggcgtg cagacagtgg atgatgttct tgagatgttt
    37861 atggctggag caagtgctgt agctataggg accgcgaatt tcaccgatcc gtatatctgt
    37921 cctaaactaa ttaaagagct ccctgtgcga atgagcgaac taggcatcga atcgctggaa
    37981 agattaatca aagaagtgag agaggaacgt gaacgatgaa tcaactagac caacgaccaa
    38041 ttattgcatt agatttttca actaggcaag aggtagaaga ctttcttcga tttttcccga
    38101 aagaagaaaa attgtttgtg aaaatcggaa tggaattgtt ctaccaagaa ggacctgaaa
    38161 tcgtacgtta tctaaaagag gctggtcata atgtattttt agatctgaaa cttcacgata
    38221 ttccgaatac ggtcgaaaaa gcaatgcggg gattggcaaa gcttggtgtt gatctcacct
    38281 gtgttcatgc cgctggcggt attcgtatga tggaggcggc aatgcgagga ttagaagagg
    38341 ggactccaga aggtggaaaa cgtccgctac ttctagctat cactcagctt acttctacaa
    38401 gtgaagaaga aatgcatgcc gatcaactaa tcgaagtttc tttagaaaaa agtgtgatcc
    38461 attatgctag ctgtgccaaa aaagcaggat tagatggtgt ggtttcttct gcttgggaag
    38521 tagaagcgat caaagaaatg gcaggagacg aatttgtttg cttaactccg ggtataagac
    38581 cagaaggaac agtcgcagga gatcagacaa gagtagtcac accttctcag gcaaaaaaga
    38641 tcggctcaac ttttatcgtt ataggtcgac caatcactca gtcaactaat ccttataaag
    38701 catatcaaac gattcaaaca gaatggagct aacctaaaat gaatgttgaa caaagtatcg
    38761 caaaagactt attagaaatt gaagcagtat ttttaaatcc aagtaatcct tttacatggg
    38821 caagtggaat caaaagccca atttactgtg ataaccgaat cacgatgagc tatccaaaag
    38881 tccgaaaaga gatcgcaaaa ggattagcaa gcaaaatcaa agaagcttac cctgaagtac
    38941 aagtgattgc tggaactgca acagcaggaa tccctcatgc cgcatgggta gcggaaatac
    39001 tggatttgcc aatggtctat attcgaagta aggcgaaaga tcatgggaaa ggtaaccaaa
    39061 tcgaaggacg tatatttgaa ggtcagaaga tggttgtgat cgaggatttg atttctacag
    39121 gcggaagtgt cttggaagca gctgaagcag caaaaagaga aggtgcggat atccttggtg
    39181 ttgcggctat ctttacgtat gagttgccaa aaggaaaagc aaatttcgaa aaagccgaaa
    39241 tccctcttat gacgttgacc aattattctg tattgattga agcagcttta gaagatcgtt
    39301 atatcgatga gcaagaatta actctgctaa aagaatggaa aaaagatcct gaaaattggc
    39361 agatttaagc gcgcttgatg aagatagaga aatccgattt gtgtctccag aatcatcaat
    39421 tggaatagca aaaatccaaa aactgattgc gagaagcatc ttttcatcag gtatgaaaag
    39481 aaacttctat cgtgatcagt ttttgatttt tttaaaattc atttttctaa gactggttag
    39541 cagaaagaag agaataatct cttctctttt ttctattata tatagatttt ctctattttt
    39601 tggtaaggga gctgtttcaa aaatggtaaa gagcggaaat gttgcgaata tcagttattt
    39661 aatgatatga tattcggaaa aagaaatctc aaagggaggt aacttttatg gggttgaatg
    39721 aatttatttt aggattgaac aatttagaga cgatcacaag ggcacctgga ttttttaaat
    39781 atacagagca tacagttgca gcacattcgt atcgtgtagc atctattgcg caagttctag
    39841 gagacattga agaagcgaat ggacaaccga tcgattggaa actgctctat gaaaaatcat
    39901 tgaatcatga ctacacagag cgttttattg gtgatatcaa aacgccggtg aaatacgcaa
    39961 gcaaagagtt aagagggatg ctgcaaacag ttgaagaaaa gatgacggac gaattcatca
    40021 accaagagat tccagctgag ttccaagaag tttatcgccg tcggttgttc gaaggaaaag
    40081 atgaaaccgt agaaggagaa ctattagcca tttcagataa ggttgacctt ctttatgaat
    40141 cttttgatga aatcatcaaa aataacccgg aaaaagttta taaggagatg ttcattgagg
    40201 cagtgactac gattaaaagc tttgaacatc ggccttctgt tcagtatttc tttaaagaga
    40261 tatttccgga actattgaac caggaatttt atggaaaaga ggcgttttta gaaaccgtct
    40321 caacgattgt aaagaaagaa gaaaaataaa aatgatatga gatgcagggg gaagtatagt
    40381 tgaaaaagaa tatggatgtc gaatggggct attctggaga caaaggtccg gaaaattggg
    40441 cgagtttatg tgattggttt gcgagaggtg cggaatttcc attgcagtcg cctattcatt
    40501 tgaccaaggg ggaagaaacc aaaatagaag gagacacgct ttcctttcat tatcaaaagc
    40561 aacggtttac tgacaaggaa ttcaaaaaca cgattcattt agtacctttt gaccaactca
    40621 gttatgtaga gttcaagaaa gaacgctatt atttgacaga tattcatttc cacttgcctt
    40681 ctgaacatat catcaacgga aaacaggaag atattgaatt tcattttgtt catatgaaca
    40741 gcaaaaaaga gaatctagtt gtgggtgtca tgtaccagct aatggtacag gaaggttggc
    40801 tgtataatca agaaaatggg gaaagttgga acttagaaaa acatgagcat tgggtaaatc
    40861 cggatgtgtt ctttccggag caaaaaagcc atttccatta tattggctca ttgacaactc
    40921 cgcctaccag cgggccaatc caatggtttg taatggatca tgtaggaaaa ctgaaccaag
    40981 agtttcttct ccccttcgat ggtcagtatg ctcgaccgaa caaccgacca atccagccct
    41041 tgaatggaag agaaatttat tatttcgagg agtttgagca atgaatattc agcagatgaa
    41101 atacgtggtc gctgtagcaa acaatggtag tttcagagaa gcggcaaaga aaatgtttgt
    41161 ctcccagcca agtctttcag ctggtatcaa agaattagaa accgagttag ggatcacgct
    41221 gtttactcga acgaatcgag gggcatattt aacagaagaa gggcaagact tcttaaatcg
    41281 tgccgagaaa atattggtcc agcttgatac attagaaact cactatctaa cgagtgaaaa
    41341 gagagacaat ttttcgatcg ctgcccagca ttatgatttc ttaggtcctt tgaccgcaca
    41401 gctactcgag gaattcaaag acgagatcaa acaatttcgg atcatggaga cgacaacagc
    41461 gagagtgatc gaagaagtca aagagcagca cagtgagatc ggcattattt atatgaatga
    41521 gcataatcaa gctggtatca gaagatattt acaacaagga gagctaagtg gccgcagcat
    41581 tggaaaattc aaaacgcata tttttttaag gcaaggacat ccattagctg aacacgcaga
    41641 gatcaccaaa gaagaattgt tatcttatcc tcaagttcgt tttacacagg atggaaataa
    41701 tttcccgtat ttttatgaag accttatcga gatccctgat caagaaacag tgatttatac
    41761 gagtgatcga ggtactttga tgaatatcgt tcttgaaact gatgcttacg cttcaggatc
    41821 tggtattgtg attggaggga ttcgcgagca cttgcggtta atccctttgg cagaaggcga
    41881 actaaatgaa ttttatatca tttattcggc gaaacgtccg ctcagtgaaa tcgctcaaag
    41941 atttatcaaa gagcttactc ggttactgga ccagcagagc taagaaaagc agatgagaac
    42001 gctgtttatt gaacaccgtt gatcaaaaaa gaggggttgt gacagaattt tgtcacaact
    42061 cctctttttg tagcaaacaa tttataacta attgctgttg tgggaaagta cgcaaatatg
    42121 attctagaaa cactcattga ttacctgttt tgtttcatag ctgtaatttt ttcttcttct
    42181 ggtgtcacga taatcgtctt ttggttttca taaataacat aaccaggctt ggcgccattt
    42241 ggttttcgga tatgctttac ctgtacaaga tcaaccggca cttgggcaga gtatcggtat
    42301 tttgaaaaat aggcagctaa ctcggcagct tctgtaattg tttcatctga gggcttgtca
    42361 cttttaatga tgacgtgcga accgggtata ttttttgcat gaagccaata gtcggttttt
    42421 ttagctgtct tcattgtcag ctggtcattt tgaagattgt ttttaccaac aagaatgagc
    42481 gttccgtcag aagaaaagta ttgatcaggc tgagatggtt tcttacgttt ttgttttttt
    42541 aagttcttct ttttcaggta tccttctgaa gtcaattctt ctttaattgc ttcgatatcc
    42601 attgggccgg caatttccag ttgagataag acagattcaa gatattggat ttcatctttg
    42661 gcttcttgga tttgttcgcc aatcagctta acagcgttct tcagcttttg gtatcgatgg
    42721 aaatatttct gtgcattttg atttggtgtc aatgccggat ctagtgctat tttgattggc
    42781 cgatcttctt cataataatt aggtaaaaca acttcttttg cgccgcgtgg tacttgtgtc
    42841 agaaaagtag taagtagttc gccatttcta cggtaattct cagcgttttc ggattctttg
    42901 agtgtttgtt cacgtttttt caatttattt ttatttcgtt ttagttcatt ttcgattttt
    42961 cgaatcagtt ctccgccttg ttgttttgcc cgatctttct ctgctttttc gtgataatag
    43021 ctatctaata attgactgag tgttggataa gctgttacag cagatgcctg ttcggacaat
    43081 acctgataag caatcggtgt gaaaaaatct ttattttccg tttcgtaaaa agtaggaata
    43141 ggctgatggc tgatggctga gaaaaattcg tcccatacaa ccatcttttc attaggacgt
    43201 tccagtaatc ttttggtcag ctcttgagcg atatcgaaac cgattccctg aaactgacgt
    43261 tggattgctt ttggatctag ttcagtttgg gaaagccgct gaaagatttt ttctttctcg
    43321 gagctaaatg ggttcaagac ctcttgtttt ggcggagcaa catattctac accaggaagc
    43381 aaagaacgat aagtgttttg agagcttcca atgtgtttga tcgcatccag tatttttcca
    43441 gtttctcgat taactaagac gatcgtacta tgacgtccca ttaattcaac gatcaatatg
    43501 atattttgta gatcacccaa ctcgtttcgt ttagcaaagt ggaaatggat cactcgatca
    43561 ttttcgattt gttcgatgtc ttctaaaata gcgccatcca aatattttcg aagcatcatc
    43621 acgaagttag gcggattatc aggattctga taatcgattt gggtaatctg tacacgagca
    43681 taacttgggt gcgcagataa taataatcga tggtttttgc cgcgtgaacg aatcaccagc
    43741 acgatttcat tttcataagg ctgatggatt ttcgagatcc ggccagatag taacgtttca
    43801 cgcaattcgt tgaccatcgc gtgggtaaat acaccatcaa aggacatggt tcattcctca
    43861 tttcttttca taagtatatt cattataacg aaaaaaagaa gaaaaaccaa tttggccgaa
    43921 tagtaaagca aaagtctaga aaaatcttgg actaattaca taaaaaacag tgtttctatc
    43981 gttgaaatcc aaatttttag gtataataaa aaataggaga aaaagtagaa aagaaggtgt
    44041 ttgttatttg ggaagtagtt aaatcgagag tacaagaaat gaatgaacat ttctcagtgt
    44101 cattcgcaga aaatccaagt aagtattttc gacgtcttgt ttttctgctc atcttatcat
    44161 tgctgttaac agttgcaatg tttttattgc cggaaaaaat ccgagaagag caagtaaata
    44221 gtctcgttta ttcgtccata ccaaccagca aattgaaacc ttttgattac agcaaagctg
    44281 atcagaaaat caaacaaagc cgggcgatat cggtgatgtt cagcaaaccg aacgggaaaa
    44341 cctatcagga catgcttgat gtttttaatg atcctgagct tatggaagaa ttgaatcgac
    44401 cgatctttta ttatccgatc gtttatgatg tacatgaact ggaacaaaaa tataacatac
    44461 gaacggatga agtgacattt attttctttg atggaggaaa agaagcaaat cggataactg
    44521 cgggtaaggg aggaataact gattttgata aggagttgat ccctgaactg aatcgacttc
    44581 cgttagccaa tatcaaacaa ttagaagaag aattggatag aacgggagta acttcccaaa
    44641 attccgagat tgctaattga gacattggcg cgatgaaacg gtgggtctat atagcgctat
    44701 ttttaattat cagaacaagt gatgtttgat cttcaaaata acaaataaaa aattaaaaaa
    44761 tatagttgac gaaattcaaa aattcgatta gacttaaatc aattcttaag gaaagggtga
    44821 cctgtaatga acttatcaaa acgtatttca gcagaaaaca aaaaaaattg gcgcagctgg
    44881 cgtagataag ttgtattaat ggtgagactt tcccatagat acaacttttt agagtgcatt
    44941 tctaaagctt gtatctatgc tagacatgaa cattgactgc cgcatagatt agaatctatg
    45001 cggttttttg tgtaaagaca atgaccgctt tggccattgt ctttttttgt agactggata
    45061 cttgcctgat tataataaat aaaaagattg actttgcagg aggaaaaaaa tgaaaaacaa
    45121 acgattaata acagttgtag taattatttt agtttattta gcaggtgcat ttatttatgg
    45181 aaaagcctca ggcgaaaaag aaagtacttc gacgaaccag acaaaaaaaa caacagttgt
    45241 tggggtattg caatacgtga gccatccggc attagatgag atttatcgag gaatcaaaga
    45301 tggtctgaaa gaagaaggat taaaagaagg agaaaatctt gagatcaaat ttcaaaatgg
    45361 acaagccgat cagagcaagc tagccacaat gagtcaacag ctagtacaat ctgatccaga
    45421 tgccttgatt ggaatcgcaa cacctgccgc tcaatcatta gcaaacacga cttcatcgat
    45481 cccagttgtg ttaggtgctg taacggatcc tgtgggggct ggattagtcg cttctttaga
    45541 gaaacctggt ggtaatgtaa caggtgtttc tgatcagccg cctgttactt cacaaatcaa
    45601 gcttgcttct gatttgttgc caaatgcaaa aaaagtcgga atcttgtatg cttcttcaga
    45661 ggataattca aaataccaag tagcacaagc agaagaagct gcaaaaaaag aagggttaga
    45721 atcagtaaaa tatgcggtgc cttccactaa tgagattgca caaacggttc aagtgatgag
    45781 ccgacaagtc gattttatct atgttccatt agataatacc attgccaatg cgatgcaaac
    45841 agtggtaaaa gaagcaaaca aagcaaatat ccccgtcatt ccatctgtgg atacaatggt
    45901 ggaacaaggt ggactagcaa caatcgggat caatcaatat caattagggc ttcaaagcgg
    45961 taagatggcg gcagaacttg cgagtggaaa agaaaaaccg gaaacgacgc ctgtttatac
    46021 atttgatcaa ggggatacag tgatcaatca atctcaagct gatcgcttag gaatcattgt
    46081 tccccaatcg atgaaggaaa aagccaaaat cattacagat gactcgcagc aagaaacatc
    46141 taaggaggat gacaaatgat cgtatcagct atttcacaag gcatgctttg ggcaatatta
    46201 ggattaggga ttttcatgac ttatcggatc ttagactttc ctgatatgac gacagaaggg
    46261 tccttcccat taggtgctgc tgtctgtgtg acagctatta ctcatggggt agcgccgatt
    46321 gtagctacat tattaggcgt aggtgctggc atgatagcag gactcgttac ggggttactt
    46381 tatacaaaag gaaagatacc ggttatttta gcagggattt tagtgatgtc aggattgaac
    46441 tcagttattt tgtttgtgat gcgaacacca aatctttctt tattgaatca tccgattcta
    46501 caagacagct tccatcgctt tgggttacct gattactttg acacagtcct tcttggcttc
    46561 atctctttgg gcatcttgat tgctgtactg ctattcttct tcaatacaga tttgggtcaa
    46621 ggctatattg cgacaggaga caacgaaacg atggctcggt cgttagggat aaaaaccgat
    46681 cgtatgaaaa ttttaggttt aactttgtcg aacggtgtca ttgcattatc cggtgcgctg
    46741 attgcccaga acgacggata tgcagatgta aataaaggga ttggcgtaat cgtcatcggt
    46801 cttgcttcca tcatcatcgg tgaagtgatc ttccaagaat tgacgctagc agaaagattg
    46861 attgctattg ttgtcggcag tatcatttat cagctattga ttttagtagt aatcaaacta
    46921 gggtttgaca cgacttatct gaagttgttc tccgctatca tcttagctat ttgtctgatg
    46981 atcccgcaat tgaagaaagc actgaactta aaaacgggtt ttgagaagga ggcatagtaa
    47041 tgactgatat attgacaatc aaaaatggca gaaaagtcgt aaacaatgga atgaatgaag
    47101 agaaagtact attggatgaa attaacctta caatcagaga aggggacttc atcactgtcc
    47161 ttggcgggaa tggtgctgga aaaagcacac tgttcaatac gatcgctggt acgcttcaac
    47221 tcagtcaagg atcgatctct tttaaagatc agctgatcac aaaagattca gaagaaaaaa
    47281 gagcacgatt cctttctcga gttttccaag accccaagat gggaacggct cctcggatga
    47341 cagtagcaga aaatttgcta cttgcacaaa aaagaggtca aaaacggaaa ttccgtttac
    47401 gtaacttgaa aaatcaaaaa gaagattttt tcagactatg tcaagaagtc ggtaatggcc
    47461 tagagcaaca cttggatact ccagcgggtg aattatctgg tggtcaacgc caagcattaa
    47521 gcttgctgat ggcgacgatc acagctcctg agttactatt attagatgaa catactgcag
    47581 ctttagatcc taaaacagct aaagcattaa tgcgattaac aaatcagcgg atcaatgaac
    47641 aaaaattgac ttgtctcatg atcacgcatc gaatggaaga cgccttgaaa tacggaaatc
    47701 gtctgatcgt actccaaaaa gggaaaatcg ttcaagatat ttctgaaaaa gaaaagcaac
    47761 atttgacaat gggcgacctt ttgcagttct ttgaagaagt agtggattga tttcgacaga
    47821 aaaattcctt tcagtagaag gacttgaaaa ctcattgaca tcttcctttg attcatccta
    47881 tacttaaaac aataaacaat taaacatatt tgggtgtttt gtaggatcac gtagtcaatg
    47941 attttaacaa ttaaatagaa tattctttgc tctgacaagt aaacgatgag aaaagaattt
    48001 gctactttgc cacctagacg tttcgtactt tttcgtatca aacgctaagt ggcttttttt
    48061 attgaagtaa gaacaaagga ggaataagat gatttctgga caaaccaaat tagctgggct
    48121 ctttgctgat ccggcttctc acagtttatc cccattgatg cacaatactg cttttgaagc
    48181 aaacaacatc gatgcggtgt atttagcttt ccaagtaaat ccaatgaatt tgaagcaagc
    48241 agtcgaaagt attcgaactt ttgatatgtt aggtgtaaat ctttcgatgc cgaataaaac
    48301 agcggttatt ccttatctgg atgaggtaag ccaggaagcg caattgatcg gagcagtgaa
    48361 tacgatcgtg cacgaaaagg gtcgtttgat tggctataat actgatggtg cgggcttcat
    48421 gcaatccgtg caagaagctg ggatttcgat caaaaagaaa aaaatgactc tactgggagc
    48481 tggaggtgca gcaaaggcag tcactgttca agcagcgtta gacggcgctg aggaaatcgt
    48541 agtttataaa cgaaacaacc aaacattcgc tcaagtaaaa gaaacattcg aacaactcag
    48601 ctatcgaact tcttgtaaga taaaagttcg tgactttgcc gatacgcatc aattaagaaa
    48661 agacttgaaa gaaagctgtc ttctagtcaa tgcaacagat atcgggatgg gaaagaaaat
    48721 caatcagtca ccgctcttag atataagcat gatgcatgag aatctagcgg tttttgattt
    48781 gatttatgca cctagagaaa cacgtttatt aaaagaagca aaaaaagtag gagcaaaagg
    48841 atacaatgga cttggcatgt tgatctacca aggagcaatc gcgtttgaat tatggacaaa
    48901 ggaaaagatg ccgatccatg tgattgaaaa gctgtttgat taaaaaagga taatagataa
    48961 agatctttta ttctcgcata aacggtctca agaaaagtaa tcctttatgt atcgaagcat
    49021 ttatttcgtt at
//
LOCUS       DAZKKW010000012        47469 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.012, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000012 DAZKKW010000000
VERSION     DAZKKW010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 47469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47469
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.012"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(54..788)
                     /locus_tag="ACG8PO_000661"
     CDS             complement(54..788)
                     /locus_tag="ACG8PO_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HFQ0206012.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1115..1525
                     /locus_tag="ACG8PO_000662"
     CDS             1115..1525
                     /locus_tag="ACG8PO_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFQ0206013.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1518..2219
                     /locus_tag="ACG8PO_000663"
     CDS             1518..2219
                     /locus_tag="ACG8PO_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HFQ0206014.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2219..3805
                     /locus_tag="ACG8PO_000664"
     CDS             2219..3805
                     /locus_tag="ACG8PO_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0206015.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3824..4045
                     /locus_tag="ACG8PO_000665"
     CDS             3824..4045
                     /locus_tag="ACG8PO_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HFQ0206016.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4042..4803
                     /locus_tag="ACG8PO_000666"
     CDS             4042..4803
                     /locus_tag="ACG8PO_000666"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HFQ0206017.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5183..5692
                     /locus_tag="ACG8PO_000667"
     CDS             5183..5692
                     /locus_tag="ACG8PO_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HFQ0206018.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5767..6306)
                     /locus_tag="ACG8PO_000668"
     CDS             complement(5767..6306)
                     /locus_tag="ACG8PO_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HFQ0206019.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6446..7057)
                     /gene="rpsD"
                     /locus_tag="ACG8PO_000669"
     CDS             complement(6446..7057)
                     /gene="rpsD"
                     /locus_tag="ACG8PO_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFQ0206020.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7319..8170
                     /locus_tag="ACG8PO_000670"
     CDS             7319..8170
                     /locus_tag="ACG8PO_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HFQ0206021.1"
                     /translation="MRGYVQMEKSEGSLRRKSMNPVSPLFEKIDASIEKKANLFENSF
                     SRYAVRAVLACLFLTLGTAVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAEL
                     GTSNMLYMTVGVFRQRLALSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNF
                     MLESIAVKLAKPSATILVEGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLG
                     YEHVIANFPAFTLTYFASHGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQ
                     SKSSYVD"
     gene            8198..8833
                     /locus_tag="ACG8PO_000671"
     CDS             8198..8833
                     /locus_tag="ACG8PO_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HFQ0206022.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8853..9626
                     /locus_tag="ACG8PO_000672"
     CDS             8853..9626
                     /locus_tag="ACG8PO_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFQ0206023.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10034..10831
                     /locus_tag="ACG8PO_000673"
     CDS             10034..10831
                     /locus_tag="ACG8PO_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HFQ0206024.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10809..11222
                     /locus_tag="ACG8PO_000674"
     CDS             10809..11222
                     /locus_tag="ACG8PO_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFQ0206025.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11206..13851
                     /gene="mutS"
                     /locus_tag="ACG8PO_000675"
     CDS             11206..13851
                     /gene="mutS"
                     /locus_tag="ACG8PO_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFQ0206026.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13869..15977
                     /gene="mutL"
                     /locus_tag="ACG8PO_000676"
     CDS             13869..15977
                     /gene="mutL"
                     /locus_tag="ACG8PO_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFQ0206027.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            15999..16559
                     /locus_tag="ACG8PO_000677"
     CDS             15999..16559
                     /locus_tag="ACG8PO_000677"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase"
                     /protein_id="HFQ0206028.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16644..18632)
                     /gene="treP"
                     /locus_tag="ACG8PO_000678"
     CDS             complement(16644..18632)
                     /gene="treP"
                     /locus_tag="ACG8PO_000678"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HFQ0206029.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18855..19568
                     /gene="treR"
                     /locus_tag="ACG8PO_000679"
     CDS             18855..19568
                     /gene="treR"
                     /locus_tag="ACG8PO_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HFQ0206030.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19645..20091
                     /gene="msrB"
                     /locus_tag="ACG8PO_000680"
     CDS             19645..20091
                     /gene="msrB"
                     /locus_tag="ACG8PO_000680"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HFQ0206031.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            20261..20863
                     /gene="ruvA"
                     /locus_tag="ACG8PO_000681"
     CDS             20261..20863
                     /gene="ruvA"
                     /locus_tag="ACG8PO_000681"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFQ0206032.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20876..21877
                     /gene="ruvB"
                     /locus_tag="ACG8PO_000682"
     CDS             20876..21877
                     /gene="ruvB"
                     /locus_tag="ACG8PO_000682"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFQ0206033.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21906..22484
                     /locus_tag="ACG8PO_000683"
     CDS             21906..22484
                     /locus_tag="ACG8PO_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0206034.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22536..23018
                     /locus_tag="ACG8PO_000684"
     CDS             22536..23018
                     /locus_tag="ACG8PO_000684"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HFQ0206035.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23135..23509
                     /locus_tag="ACG8PO_000685"
     CDS             23135..23509
                     /locus_tag="ACG8PO_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0206036.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
     gene            complement(24187..25539)
                     /gene="brnQ"
                     /locus_tag="ACG8PO_000686"
     CDS             complement(24187..25539)
                     /gene="brnQ"
                     /locus_tag="ACG8PO_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HFQ0206037.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
     gene            25686..26276
                     /locus_tag="ACG8PO_000687"
     CDS             25686..26276
                     /locus_tag="ACG8PO_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HFQ0206038.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVLVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            complement(26404..27753)
                     /locus_tag="ACG8PO_000688"
     CDS             complement(26404..27753)
                     /locus_tag="ACG8PO_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFQ0206039.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(27921..28661)
                     /locus_tag="ACG8PO_000689"
     CDS             complement(27921..28661)
                     /locus_tag="ACG8PO_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFQ0206040.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            complement(28674..30266)
                     /locus_tag="ACG8PO_000690"
     CDS             complement(28674..30266)
                     /locus_tag="ACG8PO_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327538.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFQ0206041.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     regulatory      complement(30404..30578)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            complement(30834..31130)
                     /locus_tag="ACG8PO_000691"
     CDS             complement(30834..31130)
                     /locus_tag="ACG8PO_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0206042.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     gene            31306..32031
                     /locus_tag="ACG8PO_000692"
     CDS             31306..32031
                     /locus_tag="ACG8PO_000692"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0034979 - NAD-dependent protein lysine
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HFQ0206043.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEMAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            32024..32866
                     /locus_tag="ACG8PO_000693"
     CDS             32024..32866
                     /locus_tag="ACG8PO_000693"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HFQ0206044.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELIALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            32869..33390
                     /locus_tag="ACG8PO_000694"
     CDS             32869..33390
                     /locus_tag="ACG8PO_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HFQ0206045.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(33643..34206)
                     /gene="def"
                     /locus_tag="ACG8PO_000695"
     CDS             complement(33643..34206)
                     /gene="def"
                     /locus_tag="ACG8PO_000695"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFQ0206046.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            34457..34726
                     /gene="rpsO"
                     /locus_tag="ACG8PO_000696"
     CDS             34457..34726
                     /gene="rpsO"
                     /locus_tag="ACG8PO_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFQ0206047.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            34914..37040
                     /gene="pnp"
                     /locus_tag="ACG8PO_000697"
     CDS             34914..37040
                     /gene="pnp"
                     /locus_tag="ACG8PO_000697"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFQ0206048.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            37550..37816
                     /locus_tag="ACG8PO_000698"
     CDS             37550..37816
                     /locus_tag="ACG8PO_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFQ0206049.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            37960..39951
                     /locus_tag="ACG8PO_000699"
     CDS             37960..39951
                     /locus_tag="ACG8PO_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HFQ0206050.1"
                     /translation="MATTHNTKKISMTGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGL
                     SGINPDFVIGSVSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLII
                     PAMIGGAALLADGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFM
                     VQRFGTDIVGKAFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKL
                     GLFILGNIFLATTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQN
                     PEAQQIKNLNPFFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPR
                     LKIMYPGNSIGQMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALL
                     YFYLIQNGHSRWLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWE
                     QGNIIRESSTEDVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSI
                     LDKRPKRARVYWFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQ
                     VIHDLMKQGRLPKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAI
                     KKIATSPETWFGLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            40305..41606
                     /locus_tag="ACG8PO_000700"
     CDS             40305..41606
                     /locus_tag="ACG8PO_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFQ0206051.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYYYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKTE"
     gene            complement(41736..43106)
                     /locus_tag="ACG8PO_000701"
     CDS             complement(41736..43106)
                     /locus_tag="ACG8PO_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HFQ0206052.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVNPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(43288..43611)
                     /locus_tag="ACG8PO_000702"
     CDS             complement(43288..43611)
                     /locus_tag="ACG8PO_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HFQ0206053.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            complement(43936..44544)
                     /locus_tag="ACG8PO_000703"
     CDS             complement(43936..44544)
                     /locus_tag="ACG8PO_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HFQ0206054.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEEESTGEGGEPEVIGSTEE"
     gene            44874..45590
                     /locus_tag="ACG8PO_000704"
     CDS             44874..45590
                     /locus_tag="ACG8PO_000704"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0140098 - catalytic activity, acting on
                     RNA [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
                     /protein_id="HFQ0206055.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            45603..46298
                     /locus_tag="ACG8PO_000705"
     CDS             45603..46298
                     /locus_tag="ACG8PO_000705"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HFQ0206056.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEVQPTYRIDS
                     LEELYSLLEIEKD"
     gene            46382..47011
                     /gene="udk"
                     /locus_tag="ACG8PO_000706"
     CDS             46382..47011
                     /gene="udk"
                     /locus_tag="ACG8PO_000706"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HFQ0206057.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
BASE COUNT        15389 a         8788 c         9879 g        13413 t
ORIGIN      
        1 taaaaaaact tggtacgcaa tcgctgtacc aagttttttt atttagcttt tgattaagcc
       61 ataaataata atttatataa gaatactgct acgacaccag cacagatagg tgctacaact
      121 ggtacccatg catatcccca ttgagaagaa cctttatgtt tcaatggaag aagtgcgtgt
      181 acaagacgtg gagcaaagtc gcgggctggg tttagtccag gacctgtagg accaccaagt
      241 gaagcaacta atgtccatac aaggaaacca agtgctaagt gagctgtacc aagattatct
      301 tggaagaatg gtgcttttgt aatacctagg gcaccaaaga ataaaacgaa tgaaccaaag
      361 aattcattga cgaaaccatt gaatttagaa ccaacgctgt tgattgttga gaatgtacct
      421 aacacatggt ttacgtcttc tgtttggtcg tagtaaggtt tgtaacaagc tactacgatc
      481 aattgtccaa tcgctgcacc aatcaattgt gcaatgatat aaggaacaac ttcagcccaa
      541 gggaatagac cattagatgc taaagcaatt gtaaatgctg ggttgatgtg gttaccgctg
      601 attccaccaa aaatcattgc tggcatcata accccgaatc catacccgac agcaatcagc
      661 atccagtcac tgccatatcc ttttgtacct tttaaatcta cgtttgctac agcaccgtta
      721 cctagaacga ttagtaatgc tgtaccaatc atttccgccg caagacgggt cattagtgag
      781 taatccataa atacctccaa aagttataaa aatgcaaaaa aaaaagaaac atcaacacga
      841 aaagcatcgg tgcagtgttt cgtgtcggaa ggatttctgc agtcttaaca aatgtgaata
      901 aaaaaatgaa ttccattact tataagaaac cagaccaaca gtaaaactcc aactcttttt
      961 aaaagttctc cacaaatcac caagcttaac tataaatata ccaaaaaaaa aacgtttttt
     1021 ttctcaaaaa ttgtatctta gaaacagcat gttttgaaac tttcagaaaa ggtttgttag
     1081 taaaatgaaa gctatacgta aggagtgttg tttgatgaaa ttttttcaag agtttttcat
     1141 tattattttc ttttctgtat taggagaagg catacggatt tttgctcatt tacctattcc
     1201 tggaagtatc ttaggtattc tttttttatt tttagcattt gaaatgcatc ttgttgatcc
     1261 tgaaaaaatc ggagcaactg gtgatttttt attgaataat ctagccattt tatttgttcc
     1321 tgctggtgta ggtttattgg aatattttga tgatattgct tcgatatggc ctgttttact
     1381 gggagcagtc gtcgtgtgtt ccatcgtcac gatggttgca gcgggaaaaa cggcagaagg
     1441 tgtagaagct ttgctgggct atgtccgtaa gaaaaaagca gaaagggtat ctgtaaacga
     1501 aatggaggag aatcaaattg actgagtttt ttgtgaatcc attatttggc ttaagtttga
     1561 ctttaggagt gtacgtatta tctgacaaat ttttgcggaa atataaactg cctgttttga
     1621 atcctctcgt attatcgatt gttgttatta tcttattttt gcatttttct gggatcagct
     1681 ataaagacta taatatcggg ggcaattttt tgactatgct gattactcct gcgacagtgg
     1741 cacttgccat ccctctttat aaaaacttcc gtttactgaa agagaatttt tatcctgtaa
     1801 tggctgcgat tttagcagga atagtcgcaa attgcttggt tagtgtaggg ttcggttact
     1861 tcttctctct tcataaagag atggtgatct cattactacc taaatctgta acgacggcta
     1921 tttctgtcga tttatctcat acgatgggcg gggtgaatgc cgtgacactt gctattgttg
     1981 tctctacagg tatttttggt tcgctgatcg ccactcatat tttccgccta ttcaagatcg
     2041 aaagtccagt tgctcgcggt gtggcgttag gaagtacttc tcatgctatt ggtacagcaa
     2101 aagcaatcga gattggtgaa atagaaggaa tcatttccgg acttgcgata tgcgtcaacg
     2161 gaatattgac cgttttattg ctaccgttat tctttcagcc ttttgctggc ttgttttgat
     2221 gcgaaaacca ctattttatg ctacaataac caaaacgttc gagcataaaa aaggagggaa
     2281 agacgtgtat ttgtcttccg agtatcttaa acaatttgat aaagatctat attataagat
     2341 tctcctccta gaaagttttg aggatcaagc ttggcatacc gctgcacaat tagcgcaagt
     2401 tgttcagctg gatgcccgca gcgtgtcgaa atacctgaac gagctgtcta aaaactatca
     2461 gcagtttagc ggtaagacac accctttgtt tacaaaaaat caccgttctg gttataactt
     2521 ttatgatacg ctcgattcaa ttgaacatga gcgttttttg atctatctcg tgcaaagtac
     2581 gctgaaattc cagttgctgc acgacatttt ttttgaagaa ttccatacaa tgtaccaatt
     2641 tgcgcaaaag cattacatta gtgaatctac tgctcatcga aaaatcaatg aatggaaaca
     2701 gcaactgcaa acttacggaa ttcggctgca acgaggtaca tatatcgctc aaggcgaaga
     2761 agaaatcatt cgactttact tgcatatgac attctggcag ttgtttcgag ggaaaatctg
     2821 gccatttgaa acgatttctc aaatggacgt aaagaatatg gcagaacata tcatggcttt
     2881 ttttaatgtt cgtctgaacg agatcaaaaa aagacgattg gaatatatgc taggtgcctt
     2941 ttttttaaga aaatcacaga agcattatgt tgtattgaat gagaaaaaaa gacggctgat
     3001 ttctgacaat ctgttgttcc agcgattttg ccaggtcatg gagccggtat ttcctaatta
     3061 ttttcaagta gaagacgagt taggcgcatt atttcttgta ctgatgactc gtgaagaata
     3121 ttatagtgat cctaagatac ggaagaaaat ttttgatttt catcaacaag caaaaacacc
     3181 gccctttaca gccctttcag aagcaaaagc cgctctttct ctttatcagg aagagcaggg
     3241 gctgccagca gaaaatctaa ccttcgaggc agaaaactat ttattcagca gtcatttttt
     3301 cgcctatctt tttcctaatg cgaaagaaac gattgatggc aatagtagtg attttattaa
     3361 tcatttagtt attgaaaaca aggaattgaa acaatggctc gtacattttt ttgaaagtag
     3421 acacaaacat cccaatcatc tggcatttaa aaatcatgcg tttttgatgg ggcggtatct
     3481 gactgtgttt aaaacattag gagcctttac tccacagctt ccaaagatca cgatcctttt
     3541 aatgacagat ttcccattat ttgaagagca actgttagag gagggattac ggacattttt
     3601 ccgtaatgag tatcagctga tttttctgcc aacagattat cgggggagag aagttgatct
     3661 tctgatttcg acaagtaaag tccaccgcaa accatgggcg gatcttgact attttattgt
     3721 gactgaagaa ctaaaattaa ttgattacat ccaactatct caaaaatttg aaatgattca
     3781 aaaacaaaaa ctattaaaac aataacaaag aaggagaaaa aagatgacat ttgaagaaat
     3841 tttaccagaa ttgaaaaaag gtgcaaagat tattcgtaaa ggatggagtg gatttgaact
     3901 ttatgttgtt ctcgtatccg atgatgaatt tgatggctgt ccggtgacgc cgtacttatt
     3961 gatcaaaaca agtgacgaag gtttttccag cttcgctcct acagtttgtg atattttagc
     4021 agatgattgg gcgatcgtag aatgaaaaac cagtatccag atctagctaa acaggttgta
     4081 tttatcacag gagctgcttc tggaattggt gctgcgcaag cccgagcgtt tcttgaacaa
     4141 gatgcttttg tatttggatt agatcaaaag ttcggtaaaa tggaagaaat ttctttgcga
     4201 tttcctgaaa cttttgccta cgcactaggt gatgttcgac aaatgaaaga actccaacaa
     4261 gcggtatcga aatgccaaaa tcattttggt gaagttacga ttttactgaa tacagcgggg
     4321 atattggatg cctacaaacc cttgatggaa actgacgaat cgctgtggga tctgatttat
     4381 gagacgaatg tgaaaagcat gtatcagctg acaaaaatca tccttcctga tatgatccgc
     4441 caaaaaagcg gaacgatcat caatatggct tcgattgctg gcttgattgc tggtggaggt
     4501 gggatcgctt atacaagcgc gaaacatgcg attgtcggat ttaccaaaca actcgctctg
     4561 gattacgcag gacagggtat ccgagtaaaa ggaattgctc caggagccat ccaaacacca
     4621 atgaatgcgg ctgattttga agggaaaggt gagatggcag agtgggtggc aaatgaaaca
     4681 ccagtcaagc gatgggcaca gccggaagaa gtagctgagc tgacgttatt tcttgcctct
     4741 cctcaagcaa gctacataca aggaacgatc atcccaatcg acggcggatg gttgttaaaa
     4801 taaccttgtg tttttttcaa gcttctgtat gataaaacta aagagagaaa caacggttga
     4861 tgataaaaaa agtcgatgac tgttcccgac attcgatgcc gaacagccag ttcaaaaaat
     4921 tctttttaat aatcgttaat attcgaaact aagcggcttt tcacgacctc ttgatataaa
     4981 cgatattcaa aagcttgcaa ctgcggtaac aagtccagcc aaatgaaaaa tataaggagc
     5041 tatttttatt tgaccattct tattacttgt tgcagggcag ctttttcact accttctgcg
     5101 acacttcagt tggaattttc ttcccatcct ttggtcgcgg tcattaccaa gataaaatta
     5161 tcttgcaaag gagaaataga acatggaaaa aaataatacc cgttcagcca aacgctggac
     5221 aacgacagat gtgactcgga tggcgctagt cactggttta tatgtggcag tgacgctggt
     5281 tttgtctgtc tttagctttg gagtgattca gattcgtctc tcggagatgt tcaactatct
     5341 gtccttgtat aacaaaagat acatcgtcgc ggtaacagcg ggagtagctt tggctaatat
     5401 tgcttctcct cttggactga tagatgttgc agtaggaagc gtcagcacgc tgcttgtatt
     5461 gcttatcaac tataaaatca ctagtcgcat caaaaacatg aaaatcaaaa tggccgtgac
     5521 agcatgcgta tttgcttttt ccatgtttac agtagctggt cagctaacga ttttatatca
     5581 actacctttt ttcttgaatt ggtgggtgat tgctttagga gaattggcat caatgattgt
     5641 cggtgggata attatttatt ggatcggtaa aaaagtggat ttgtcaaaat aaagatctgt
     5701 ttaatttaaa atagggcagc atggaagaga ctgaatgttg agtcttcttc catgctgctt
     5761 ttttgattaa tttgtatgaa gaaatcgatt tagccgtttt ttcatcaaga taccgatata
     5821 agctgctcca gttgccttga ttgcttccgg cagtaaaaac ggcagtagtc cactggcaaa
     5881 tgcagttgta aaagtaagat caccacttat tttcagccag atcgttccgc agatcaatgc
     5941 agcaagagag cccattagat tggcaaagat tgcatataag taatgagcat ccgttctttc
     6001 gatcatccaa ccagttatcc acgtttgtaa gagaaatcca atcaaatatc cgccagttgg
     6061 cccaaataaa acagctatac ctccagtcat ccctgaaaat acaggaagac caatcaagcc
     6121 aagtaaaaga taaactccaa cagatatcaa tcctagtttt cttccgagaa aagttgctgt
     6181 taatccaata gcaaatgttt gccctgtcaa aggaaccaaa ccaaaaggaa tggtcatctg
     6241 agaaaggaca gaaataatcg cggcaaattc cgcagcaagt atcaattctt tcaatgttcg
     6301 gttcataacg ccctcttttc gttaacttaa tatttttatt tggttaacga aattgtaaaa
     6361 caaagtgatt actttgtaaa tagaaaatat aaaaaaagac cagctatcca atagatagcg
     6421 gtctttttca agaaagcttt tgaaattata agctttggtt gtagtattca acgacaagag
     6481 cttcgtcgat ttctggatat agttcgtcac gttctggtaa acgagtcaat gaaccttcca
     6541 atttttcagc gtcaaagctt acgaatgctg ggcgtccaac agttgcttct acagcttctt
     6601 ttactgttac gatgttttgt gatttttcac gaactgagat cacttgaccc acttctacgt
     6661 ggtatgaagg gatatcaacg cgtttgccat caactaaaat gtgaccatgg ttaactaatt
     6721 gacgtgcttg acgacgagtc gttgcaagtc ctaaacggta aacaacattg tctaaacgtt
     6781 gttctagtaa gatcatgaag ttaacaccat gtttaccttc tttgattttg ctagctttta
     6841 cgaacaagtt acggaattga cgttcgttca ttccgtacat gtgacgaagt ttttgttttt
     6901 cagttaattg catgccgtat tctgattgtt tgccacggct attaggtcca tgttgacctg
     6961 gtgcgtaagg gcgacgtgct aattcttttc ctgtgcctga caatgagatg cctaagcgac
     7021 gagaaatttt ccatgatggt cctgtataac gagacattaa aatttcctcc aatgaatata
     7081 agtttttgga gtaaaataat ccgttgaaaa attcataatc atatagtttg ttcttcaatc
     7141 ttcacctttg cagccgcggt tacacaatcg aacccgagac ggggcaacaa actagggatg
     7201 agttattatt tttctgctgc attattttac acaaattaca gtatatgata gatcatccac
     7261 gaatgtcaag ataattagta cataaaaacg ctttacaaac ctaggctgtc tttataaaat
     7321 gagagggtat gtgcaaatgg aaaaaagcga aggaagttta aggagaaaat caatgaatcc
     7381 ggtatcacca ttatttgaga aaatcgatgc ttcaattgaa aagaaggcga atctttttga
     7441 aaacagcttt tcacgttacg ctgttcgagc ggttctcgcc tgtttgtttt tgactttagg
     7501 aacggctgtg gcatttgcga tcgcgatgaa gggggaagat atcgcgcatg gtctaggaaa
     7561 gattttatac gcatttatgt tcagctgggg tctggtcatg attctataca tgaacgccga
     7621 attaggaaca tcgaatatgc tttatatgac cgtgggtgtt tttcggcaga gacttgcttt
     7681 aagtaaggca gccaagattc tatttgcctg tatttttttc aatatgattg gcggtatatt
     7741 atgtggattt ctaatttcta tgactacgcc atttcaagat ttaacgttgg acaactttat
     7801 gttagaatca atagcagtaa aactagcaaa accttcagca acgattttgg tggaagggat
     7861 gtttgcgaat atcgttgtca ataccgctgt actgatcagc atgcggatga aagaggatgt
     7921 cggaaaagtg atcacagtcg tattcatcat tttcattttt gcttttttag gatatgaaca
     7981 cgtgatagcg aattttcctg cctttacgct tacttacttt gcttctcacg gacagatgga
     8041 tggaatgaca gtcagcaatg tgttacataa tttgtttttt gctttggcag gaaattatat
     8101 tggtggcggt ttagtaatgg gactgggata tgcttggctg aatcagtcaa aatcatccta
     8161 tgtggactaa aaagtcgttc attaaagggg aagtgccatg gaagttttag tttttccaaa
     8221 aaatgaggaa aaagcgttac aaatggaaaa gtacatgaaa aatctttttt cctttgctgg
     8281 agtacctaag ccggaacgcg ctcttttaca acaaccgtta ctggatgaga gcaaaaattt
     8341 atctatttct gaagttttag cttttgttca ttattattat gaacaaatcg aaagagagta
     8401 tcagtatgta gcgatcgatt tagtccagaa aaacatcaaa cggcttacat ttgaagactt
     8461 agtcgtattg ttgccactag ttcagaaaaa agaatggtgg gacagcatcg actcttggag
     8521 aaaagtatac agcacatggt gtaaagccta cccagagcag cgtgaagaag tctttcaatt
     8581 gtttgaaaaa aaggaagatt tttggctacg tcgaatagca atcacgttgc aattaggtta
     8641 taaacagcaa acagacacag gactattgat ccgagcaata gacgaagatc gtcaaacaaa
     8701 ggaattcttt atccaaaaag caattggttg ggcattacgc gattatagta agaccgatgc
     8761 cgattgggta aaaagacagc tgacaacaga tttgagtaag ttagctgttc gagaggcaag
     8821 taagtattta taagaaagcg agacgacaag agatgagacg caaattattt gcttttgata
     8881 ttgatggaac gttgttagat tcaaacaaac aagctttaga tagcacaaga gaagcattgg
     8941 aaaaattacg caaagccggt catctggtca cgattgctac agggcggagc cgatttcatg
     9001 cgcaagagat cattcgcgat cttgatttta cgaactacat tttatgtaat ggagcggccg
     9061 gttttttaga ccatgaacaa gtatataaga atttactaga tcgcgaccag ttggatgcgt
     9121 tcgttcaaga agcgcatcat gagcagatcg atacagcttt tgtcagctta gacaatatca
     9181 agcgttcttc ttccaatgat gtaacggtga tggaagaagc gatgcattct ttcggtgctg
     9241 tcttgcctga attagatcag ttttttgcag aggaacaaga aatataccaa gcgttagcat
     9301 ttttcgatca aacatatgat gggaagttct cttcatatga taaaattcga tttgtccgct
     9361 ggcatgagaa cagtgtagat gtcgtaccta aaaatggttc taaagcagcg acaattctgc
     9421 acttagcaga acaagtaggc atccctcatg aagacatcat cagctttggc gatggacaaa
     9481 acgaccgtga aatgttgcgc atgtcaggaa ttgggatagc tatggggaat gctgtgccag
     9541 aagtacaagc agaagctaag atggtcactg atacaaacga tcaggatgga atttggaagg
     9601 cattgaaaga gttaagcgtg atttaaaaga ggttgtgaaa aagctgttct gcatcaagta
     9661 ataagcccag ccaaacaaaa atagctcctc ctatttttcg tttttctggg cttattaccg
     9721 cagatgcaag ctttcgaaca ccgtttaagg aagaggtcgt gacagaagtg gtcagtttca
     9781 agaaataaga agggatttac gaaaattgct tctcaaattt ttgtaaaatt acggcttatt
     9841 tctgaagaag tagatgaaac aacgtttatt cgggaaatgg gactgtgaca agatgtttgt
     9901 catagtccca tttttttatt ttatttttca aaactaagaa ataccaggaa agagttcctt
     9961 ttgtttgacc ttttctttct tattgaaaac aactataata tagagtgatg ttaaaagaga
    10021 agaggaattt agtgtgcggg tattgtttat tggtgatgta gtaggttcaa tgggacgaga
    10081 gatgattact gaatatcttc ctagattgaa gaagaagtac cgtccgcaag taacgattgt
    10141 aaatggtgag aatgccgctt ctggacgtgg aatcacagaa aaaatctata aaaaattttt
    10201 gcaagacggc gtagatgtcg tgacgatggg gaatcatacg tgggacaacc gcggaatctt
    10261 tgagtttgta aatgaagcta aaaagatggt tcgtccggca aattttccgg aagggacgcc
    10321 tggacaaggg atggtctttg tcaaagtgaa ccaagtcgaa ctggcagtga tcaacatgca
    10381 ggctcgttct tttatggtgg acttggatga tccttttcgt aaaatgaatg agctggtaga
    10441 agaagccaga aaacgtacgc cgatcatttt tgtagatttt catggagaaa caacaagtga
    10501 aaaacaagca atgggctggt ttttagatgg gaaagtttca gcagtggtcg gtacacatac
    10561 acacgttcag acaaatgatg cgcgaatttt gccaagagga acagcgtatc tgacagacgt
    10621 tgggatgaca ggtccctatg atggtatttt gggtatgcgc agagaacctg taatcgaaaa
    10681 gtttctgact gcattaccaa aacgttttga agttgtggaa caaggccgat cgattttaag
    10741 cggctgtatc ttggaattgg atgatacgac aggtcatgca aaagaaatcc agctgattca
    10801 gatcaacgat gatcgaccgt tcatggagta gaaagagagg cagttttttg cagcaggagc
    10861 cggaaattca aaaagaagta aggaaactga caaaactttt aagagagaat gaaacgatca
    10921 ttcgctacaa agaacttgaa gaaaagatcc agcaaaacca gtatttagct gagttgagag
    10981 aaaaaatcaa acaagcgcaa aaagatgctg ttcattttgc ccactatgac aaaccagcag
    11041 cagaaaaaga agcaatcaaa caagcggatc aattcatgca agaatttgat cagcatccgc
    11101 ttgttgtagc ttatagaaaa cagctgttgg aagcagatga tctgcttcat catttaacaa
    11161 ctatgataca agaagaaatc aatggacaaa tagaggagga aaaacatgcc tcaaaaaact
    11221 aaaaatactc ctatgatgga acaatatcta agcatcaagg cccaatacca agatgctttt
    11281 ttgttctatc gtttaggaga cttttatgaa ctattttacg aagatgcgat caaagcgtca
    11341 cagcttttgg aattaacgtt gacgagcaga aatcgaaatg cagaagaacc tattcctatg
    11401 tgcggtgtac ctcaccatgc agcacaagga tatatcgatg ccttgattga aaaaggctat
    11461 aaagtagcga tttgcgagca ggtagaagat ccaaagacga ctaaagggat ggtcaagcgc
    11521 gaagtgatcc agctagtcac tcctggtaca gtcatggaca gcaaaggatt gtcggcaaaa
    11581 gacaacaatt ttttaacagc tgtagttagt caaggaaatg aatttggctt tgcttatgcc
    11641 gatttaagta cgggagaatt gaagacggct cggttgcatg atgaagaagc tgttttaaat
    11701 gaagcctctg cattgcagac aaaagaactc gtattaggca gtgagatcac tgataccctt
    11761 cgtgaacagc taagcgggcg tttagggcta gtcttttctc aacaaaatga gatggaagaa
    11821 aacgcggaat ttagcttttt gacaagcgaa ctagtcgatc cgctggaaaa agaagcaacc
    11881 ggcaaactat tgacttattt atcggtgaca caaaagcgga gtttagctca tatccaaaaa
    11941 gcagtagaat accaaccaga tcattttttg aaaatggatt attattcaaa attcaaccta
    12001 gaattaagcc aatctattcg gactgggaaa aagcacggca ctcttctatg gttgttagat
    12061 gaaacaaaaa cagcaatggg tggccgttta ctaaaacaat ggctagaccg tccgcttatc
    12121 caggcaaaac aaattaaagc aagacaagaa atggtcgctt ctttgctaga cagctatttt
    12181 gaacgtatcg atttgcagtc agcattaacg aaagtctatg atctggaacg cctagcaggt
    12241 cgagtagctt tcggtaatgt caatgggcga gacttgatcc agctgaaaac ttctttagaa
    12301 caagtgccaa tgatcagagg gttgattgaa ggaatcgatc aaggtcaatg gcaaagtctg
    12361 ctttcagaaa tgcagccgat ggaccatctc gtccaattga tcgaccaagc tatccaagat
    12421 gatccgccgt tacaaataac ggaaggaaac atcatcaaag atggattcaa tgagcagctc
    12481 gatatgtacc gttctgctat gagaaacgga aagaaatggt tggcagaact tgaagcaaaa
    12541 gagcgtcaag aaacaggaat caagaactta aaaatcggat ttaaccgcgt tttcggttat
    12601 tatatagaaa ttacaaaagc aaatcttgga aatgcagaac tggagaagta tgaacgaaaa
    12661 caaactttag caaatgcaga gcgtttcatc acaccagaac taaaagaatt ggagacacaa
    12721 atcctcgaag cagaagaaaa atctgttgat ttagaatatc agttgttttt agctgtgaga
    12781 gaagaagtca aaaaggctat ccagccgcta caagtacttg caaaagcaat cagtgcagta
    12841 gatgtgctac aaagttttgc aacgatcagc gaacgctatc agtatgttcg gccagaactt
    12901 gtcagtgaca agcatcagct tctgatcaaa gacggacgac atcctgtagt agaaaaagta
    12961 ttaggacatc aggaatatat ccctaacagc gtagaaatgg cagaagacga aatgatcttg
    13021 ctgattacag gaccgaatat gtctggtaaa agtacgtata tgcgacagtt ggcattaact
    13081 gttctgatgg cgcaaatggg ctgtttcgtt ccggcaaaac aagcagtttt acctattttt
    13141 gatcggattt ttacccgaat tggtgcaagt gacgacttaa ttgctggaca aagtactttt
    13201 atggtagaga tgatggaagc caatcaagcc ctgcgttatg ctacgccaaa cagtttgatt
    13261 ttgtttgacg aactagggcg aggaacagca acctatgacg gaatggcgtt ggcccaagca
    13321 atcatcgaat atatccatcg tcatgtacaa gcaaaaacac tcttttctac tcattatcat
    13381 gaactgacgg tacttgagaa agaacttccg cagcttaaaa atgtccatgt tggagccgtt
    13441 gaaaaagacg gagaagttgt cttcttgcat aaaatgatgg atggacctgc ggataaaagc
    13501 tacggcatac atgtggcaaa gatcgcagga ttgccttcca atctactaga acgagcagca
    13561 gatatcctgc atacgctaga aaatggcgat aacggacacc atactgttcc agctgaagta
    13621 gaagagaaaa ctaaaacagc caaacaggag caaataaaac cacctgcaga aaaagtaaat
    13681 caagaccata tacaagaccg tgtaaaagaa gaaaaccaac agctttcttt attcagtgaa
    13741 ttatccgaaa atgaaacaga agtcattgaa tcactgaagc aaataaattt actagaaatg
    13801 acgccaatgg atgcactaaa cacattatat gaattacaaa aacaactgta ggaagaaggc
    13861 ggatatcgat ggccaaaatc caagaattat ctgaacgttt agctaatcag atcgctgcgg
    13921 gagaagtagt ggagcgacca gcttccgttg tgaaagaatt agtggaaaat gcaattgacg
    13981 ccggcagtac tcagattgat atcttagttg aagaagcggg actgcggacg atccaagtga
    14041 ttgataacgg agaaggcatt ttggcagacg acgtagagaa tgccttcaag cggcatgcga
    14101 caagtaagat ccataatcgc gacgatcttt ttcgcatacg aacgctgggc tttcgagggg
    14161 aagctttgcc aagtattgcc tctgtttctg aaatgactct ggaaacggcg aatcaggaag
    14221 aaaaacaagg cacctatctg tcgctgaaag gcggagaagt tattgagcat cgaccagcac
    14281 cactgcgtca aggaacgaag attaccgtca gcaatctgtt tttcaatacg ccagcccgct
    14341 tgaaatacgt gaaaacactg cacacagaat tagcgaacat cggggacatc gtcaatcgac
    14401 tggcattgag tcatccatcg attgcttttc gtttagtaca tgatggaaac aaaatgctgg
    14461 caactgctgg aaatggcgat ctgaaacaaa caattgcggg aatctacgga ttatctaccg
    14521 caaaaaaaat gctgaaaatc gaaggaaaag atctagattt tgagttattt ggctatgttt
    14581 cattgcctga ggtgactcgt gccagtagga attacttatc gacgatcatc aatggacgtt
    14641 ttatcaagaa cttttccttg aataaagcaa tcgttgcagg ttatgggtca aaattaatgg
    14701 tcggtcgttt ccctattgct gtattggaaa tcaaaatgga cccgctttta gtagatgtaa
    14761 atgttcatcc gacgaagcag gaagtccggc tgtcgaagga aaaagaacta acagagctaa
    14821 ttagtaacgc cattcaaaaa gcattgtctc aagaaaatct gattccaagt gcagcggata
    14881 atctacggtt taagaaaaaa gtacctgatc agcctaaaac agaacaaatc gaaatcgatt
    14941 tgtcctacgc ttttgaagaa gaaaaaccta aaaagccaag tagtttgaat tttgatcgag
    15001 agacaggtac ctttttcgta gaagaactaa agaacacatt tgttgacaga attgttgata
    15061 agtctgtgga aaatgttgaa aactattctg gtttttctga agaaaacacc acttatccag
    15121 aaaaaacagc gaaaacggtg gataagtttg gtgaaaatgt tgataacttt gtagaaaaaa
    15181 cagtggaaaa atctgaaaag aaagaatttc ccattgtgga taacatggag aaaacaaatg
    15241 aagaacaagc agataaatcc gtagaagagg atgaaaagat ttacgaggaa gaattgacac
    15301 tccatcctga atttgatttc actcaaggaa acgacaaaac attagaacaa gccatcaaga
    15361 aattagaaac ggaaaaacca gtacaacgtt ttcctcagtt ggaatatttt ggacagatgc
    15421 acggaacgta tctttttgcc caaagtagcg atgggttgta catcattgat cagcatgctg
    15481 cccaagagcg cattaagtat gaatatttcc gggaaaaaat tggggaagta tctgatgatc
    15541 tgcaagaatt gcttgttcca tttgtgttag actatccaaa cagtgatgct ttgaaattaa
    15601 aagagcaaaa acacttgcta gaagatgcag ggatttattt agaagaattt ggtcaaaaca
    15661 gctttattgt acgggcccat cctacttggt atcccaatgg agaagaagaa tcgattattc
    15721 gggagatgat cgatatgctg ctgacaactg gaagtgtgag tgtaaaaaaa ttccgtgaag
    15781 caacagcaat catgatgagt tgcaaacgat cgattaaagc caaccattat ttgaatgaag
    15841 cacaagcacg tgtgctgctg aaggatctgg cacgatgcga aaatcctttc aattgtcccc
    15901 atggccgtcc agtattgatt cactttacca attcggatat ggaaaggatg ttcaaacgaa
    15961 tccaagaccc gcattaagtc aaagagaaga gggaaaacat gaagattgta ttagcatccc
    16021 aatcaccaag aagaaaagag ttattggctc atatcgtttc agaattcgag attgaacctg
    16081 ctgatatcga tgaaacgcca tttccagacg aacagcccgt cgattatgtt cgtagaatgg
    16141 ctgaagaaaa agcacgaact gtctgggaac aagatgaaaa agaagaccat ttagtgattg
    16201 ccagtgacac gactgttgtt ctcaatcaag agatcatggg taaaccagaa gatctggctg
    16261 aggctgaatt catgctgaaa aaactaagcg gggaaacaca tactgtatat acagctgttg
    16321 ttttaaaaac aaaggaacga gaagaacgga tactagcaga agcacatgtc actttttatc
    16381 ctttgacaga cgaagagatc aaacgctact tggagacggg ggattatgcg gataaggcag
    16441 gggcatacgg catccaaaat gctgcgagtg tattcgtcaa agaaatttct ggagattatt
    16501 acagtatcgt aggatttccg attggtgctg tcaatcaggc actgaaaaca ttcatttaaa
    16561 caaaaaagcg gactatctga ttttcgattg aaaatccatg atagcccgtt ttttttattg
    16621 aaaagattac ttatctactc tgactaagat tttacttcag ctttaccgac tgcttcgccc
    16681 gggcgaacaa gtcctgattg gtcaagagaa agtcctgcaa ctttttcatt attggtcaaa
    16741 gcaacaataa tgtcagtttt cttgccagct tgttctattt ctggcaaatc catttcagca
    16801 atcagggtct ctggtgtgac aaggtatcct tctttcacga atacattgaa aggaacaccc
    16861 tttagttcaa cagtatctaa tcccatgtga acaagtactt ccagcccctc attagaaaga
    16921 ataccaatgg catgttttgt ttcaaagaca ctcgtcactt taccgtttac tggtgcatag
    16981 actttgccgt ttgacggttc gacagcataa ccgtcaccca tcattttttg tgagaaaact
    17041 gggtcattta cttcagttat ttccttgatg gtccctgcag cgactgcgaa taatgtttct
    17101 tttgttgcaa ctgccgtacc agttgctgaa acaggttcga aagactgtgg cgttgctgtt
    17161 gcgccatttg ctgtttgaag ttgtacatca gcagcatttt caggaacagc aggatcaatc
    17221 ttattcaaaa ttccctgacg tctaaagatg atcgtcaagc caaatggaat gatgacagca
    17281 atgatcattg cgatgatgaa aggtacccat gtttctgctt ggatagctag tattcctggc
    17341 aaaccgccta ctccgatcgc gttagctcga acacccatca agttagcaaa catgccagct
    17401 aatccagagc cgaccattgc agcgacgaaa ggatagacgt atttcaaatt gattccgaac
    17461 atagcaggtt cagttacgcc taagtaacaa gaaatcattg ctggaatcga gatttgttct
    17521 tcttttttat tcccacgatg taagaagatg atggctaaga ctgcagagcc ttgggcgata
    17581 tttgataaag caatcatcgg ccataaattc gttgatccga aatctgcaat cagctgcata
    17641 tcgatggcat tggtcatatg gtggagacca gtaatcacta aaggagcata caggaatccg
    17701 aatacggcac taaacaacca gctgatagaa gacgtcaatc cagtatttac gatatttgag
    17761 atccagcttc cgactgtcca gccgatagga cctaaaataa cgtgtgcagc taaaacagtt
    17821 ggtaacaatg aaaacaatgg aacaaaaatc atcgagatcg actgagggat atatttgcgg
    17881 aagaagattt ctagatacgc taacataaac cctgccagca tggctggaat aacttgcgct
    17941 tgataaccaa tcatctggac ttgagcaaaa ccaaaatccc aaaatggaat atctgctgcg
    18001 gctgtggaag ctacactata agcatttaat aattgcggag aaactaacgt aattcctaaa
    18061 acgattccta atatttgtgt agtacccatc ttttttgcaa tactccaagt gatacctact
    18121 ggaagaaaat ggaaaattgc ttcaccgggt aaccacaaaa aggcattgac accattccaa
    18181 aacttcgagt gttcaacgat tgttccacca agactttcaa actggatacc ttcaagtaca
    18241 tttctgaatc caaggatcaa tccaccgaca atgattgcag gaatcagtgg tgtaaagatt
    18301 tctgctaaaa cggcaatagc acgttgaacg ggatgaaggt tttgttttgc agctgctttt
    18361 ccttgttctt tggaaacccc ttcaactcca gatactgcaa cgaagtcgtt ataaaaagta
    18421 gaaacatcgt ttcctatgat gacttggaac tgtcctgcat tcgtgaacat cccttttacg
    18481 ctaggtatat cttcgatcgc tttttcgtcc gcttttcctg gatcattcag gacaaaacgc
    18541 atacgagtcg cacaatgact aactgcagca atattttctt ttccgccgat atcatgtaac
    18601 agcttctcgg catctgcttg atattttgcc atatcaataa acccctttct cttaacttgt
    18661 ctatgcaagt tgtttcttca tttactatac tttttttcgg agagaattgc aagcgtttta
    18721 ttaaaaagta cgagagaaaa tgatatcgtt ttaaaaagct taaaataagc ttttcttaaa
    18781 aataggaaca ttgaaataca aaaagagctg tagtatagtg aagaaatgat ggaagaacga
    18841 gtgagggaaa aaacatgaat aagttcaatg aaattttttt agatttggaa cagaaaattt
    18901 tagcgaaaga atatcctccg cattcgcttt tacccagtga gaaccaattg atcaaaattt
    18961 atggtgtttc acgagaaaca attagaaaag cgctgaatct tttgactacg gaaggttata
    19021 ttcaaaaaaa acaaggaaaa ggttcgatcg tactggatcg gaatcgcttc gactttccta
    19081 tttctggatt gactagctat aaagagctac aggagaccca acgtattcca agtgaaacga
    19141 tcatccatac gttagaagaa acagaagtga caaaagcatt gaacgagatc actggctggg
    19201 agatcggtgc gcctgtttgg catttgatac gcgaaagaaa aatcaatggc gaagtagtta
    19261 tattagacac cgattatcta ttaaaggaaa tcgttcctat tttaactcca gcacaagcac
    19321 aaggatcgat ctatgaatat tttgaaaatg agctttcttt gacgattgct tacgcgcaaa
    19381 aggagatcac agtagaagaa gtaacggaaa tcatgaaaac agcgatggat cttcacgatg
    19441 acacttatgc agtagtagtc agaagcctcg ttcatctgga agatacccgc tgtttcgagt
    19501 attctgagtc ggttcaccga ttagataaat tccgtttcgt tgagtttgcc cgacgccgaa
    19561 aagtgtaaat agttcaaaag aaagcaaatt gttggcatct gtccactttg gcgctatgat
    19621 aggatcacaa agggggcatt tttaatggat aaaccaataa gaccagaaaa agaagaactg
    19681 aaagaaaagt tatcacccat ggcttatgca gtcacacaag aaaatgcaac agaacgacca
    19741 tttagcggga aatatgatga tttttacgaa aaggggatct atgtagatat cgtcagcggt
    19801 gaaccgttat tcagctctgc agaaaaatat gatgcaggtt gtggctggcc atcattcagc
    19861 aaaccaataa cgaaaagaca agtgcgcgaa aaagccgatt tttctcatgg catgcatcga
    19921 atagaagttc gaagcaaaga agcagattct catctcggac atgtgtttac cgatggacca
    19981 gtagatcaag gtggtctacg ttactgcatc aattctgcct cactgaaatt cattccatat
    20041 gaaaagatgg atgaattagg ttatggggaa ttcaaatcac ttgtagaata atggaaatac
    20101 acaaagagcg gtacaatacg gggatttact cgatcgtccg ctcttttttt attcaagcga
    20161 ttgctatttg aaagaaacgc tccctagact ccttttttca caaggtgaaa tgtgctacaa
    20221 taaagggaac gtatgtgtgc aaaagaaaga ggactagaat atgtatgaat atctgacagg
    20281 tgtggttacc tttatcaacc cctattacat agtagtagaa accaatggta tcggctacca
    20341 gatcgcacta ggaaatcctt accgctacag tagcaaacta aatcaaaaaa taaaaattta
    20401 tgtccatcag gtaatacgtg aagatgcgca tcttctgtat ggatttgatt cgctagaaga
    20461 aaagcaactg tttttgcgat tagtaagtgt ttcaggaatc ggtccgaaaa gtgcccttgc
    20521 aatcatggca agcgaagacc actccggttt gattcaagca gtagaatcca gtgatgtcac
    20581 ttatttgacg aaatttccag gagtagggaa aaagacagca caacaaatga tcttggatct
    20641 aaaaggaaaa ttcggtgagc tgagtatcga tacacctttt agtctatttg atgaagcaaa
    20701 cacaaaagac gctacagctc tatctgaagc aatggaagct ctctcggctc ttggttacag
    20761 tgatcgagaa atcaaacgtg tagaaaaaca attaaaagaa gtagataatc agacaacgga
    20821 tgagtatcta cgccaagcac tcaagctaat gatgaaaaaa taaggaggag accaaatgac
    20881 agatgaatac ttgttatcac cagaatctgg cgaaaatgaa ctgagtttgg aaaaatctct
    20941 tcgaccacag ctgctttctc aatatatcgg acagagaaaa gtcaaacaag aacttcagat
    21001 ttacatcgaa gctgccagaa atcgggaaga acctttagat catgttctgt tgtacgggcc
    21061 tcctggatta ggaaaaacga cgatggccat ggtcattgct aatgagatga ccgtaaatat
    21121 tcgtacgacg agtggccctg ctatcgaacg tccgggtgac ttagtagcaa tcttgaatga
    21181 actggaagca ggggatgtcc tgtttatcga tgagatccat cgattaccta gagtggtaga
    21241 agagatgctt tattcagcta tggaagattt ctatgtagat atcatggtag gacaagggcc
    21301 aaccgcacat ccggttcatt tccctcttcc tccctttact ttgattggcg cgacgacacg
    21361 ggcaggtatg ctttctgcac ctttacgaga tcgatttggc atcatttctc atatggaata
    21421 ttatgacgaa caggatctgc aggagatcgt tttacgttct tcggatattt ttcaaacaga
    21481 gatcattgaa gaaggggcat tggaaattgc tcgccgatca cgaggaacgc ctagaatcgc
    21541 taatcgcttg ctcaaacggg ttcgtgattt tgcgcaagtc caaggagacg ggaaaatcga
    21601 tcgtcctata gcggatcagg cacttacttt gctacaggtc gatcaggctg gattggatta
    21661 tgttgaccag aaattgttgc gaacaatgat cgagttatat ggcggcggac cagtagggtt
    21721 aagtacgctt tcagtaaaca taggagaaga gcgtgagacg gtagaagata tgtatgaacc
    21781 gtatctgatc caaaaaggat ttttaaagcg tacacctaga gggcgaattg ctactgctta
    21841 tgcctatgaa cattttggtt acgactatca tatatgattt ttgcttttag attggaggaa
    21901 aatgaatgag tgatacaaca cgtacgaaac aattgatcaa aaacgtattg attgatttgt
    21961 gccaaacgca atcttttcga aaaatcagcg tccaaaaaat cgcaaacgaa gcaaaaatca
    22021 atcgacaaac attttattat cattttacag ataaatacga tttgcttcgt tgggtctatt
    22081 gtgaggatgc attgcattat ttagatccac aatgtctttc acttgataat tgggaagagc
    22141 aagcattgaa aatgctgaaa gcaattgctg agaagaaaga attttatgca aatactgtgc
    22201 tttctgaccc agaaatttta caaaaagagt tttctcaact gatccaaaaa tcatttatca
    22261 agatttttga acaaatggat gaagaaagac aactttctgc aagagacaaa ctattttatg
    22321 ctcgtttttt ttcttacggt tgcagtggtg tattagtcaa ttggatcacg aatgaatatg
    22381 aagaaacacc gatggaaatc gctatgcagc ttttccgttt agccaaggat acggaattat
    22441 attcttatcg tctttatgca aaagaagagt tggaagaaca gtaatagaaa aattttttgt
    22501 tggaaaagca ataatcgcta aaaggagatg gggaaatgac aaaaatttta tttgtttgtt
    22561 tgggaaatat ttgccgttca ccgatggcag aaggcttgct gagaaaacga atagcattag
    22621 aaggaaggga agcagaattt tttgtggatt ctgctgctac aagcacgtat gaagttggaa
    22681 agacaccgca ccctggaacg aaaaaaatat taaaccaaga aaaagtagat atgacgaata
    22741 tggttgctcg gcaaatcaca ccccacgatt ttgaaacttt tgattggatc attggcatgg
    22801 atcaagaaaa tgtagaagaa ctgaaacgaa gagcaccacg ctcagcgcag gggaaaatcc
    22861 atttgttttt aagtagcatt cctggaaaag aaaaagaaaa tgttcccgac ccatactata
    22921 ccaataactt tgatgagaca tatcacttga tcacagaagg cctgaatcat tggtggaata
    22981 tttggattga tgaaaataaa ataacaaaca atttgtaaag ttaacgcttt ctttttaagg
    23041 cgatgttaga gcatttcctt gtttttttgt aaaatgttag gtaaaataaa attaattaag
    23101 taacactttc ttagctaaag gaggagtgca ttcgatgaac gaagagacag tatcgccaat
    23161 gaagaagctt acgaagttag ttactcatac ttcgtatcaa ctttttgatt cttttgtaaa
    23221 tgacactgaa gaacatgcta agaatgtacg attgcaagga aagtttattc ttgagctgca
    23281 aaaagctgct actgaacata gtctagttat cttacaagta agagcaaatc gtttgagtag
    23341 caagtatgaa acgatctcag gctgggtggc gacaaaaacg gtcgaaaaag accgagtgat
    23401 gatccgccta caaaacgaag agcagcagat ccggatggtt cctgtagaac aaattgaaaa
    23461 aataacaatc ctttcaccag caggggaaca ggaacggtta tcaaaataat ttataccaga
    23521 tagaaaagca cgtatgagct tgtaccatca atcattacta atcaaaaata accgaatgct
    23581 ttaagctgat atcagtaaaa cggaacgttt taggatgagt atcacagcat gacagcattg
    23641 ggttgattag ttgaatcgat tggatggtac aagttttttt gataaaaaaa ggtcctgcta
    23701 gccggggagc tagcaggata aggagttaaa aatgaaaaag tgttgttagg gttgtttgtt
    23761 ggtatgctta tataatagtc actagttgtg aatattttgt gacgaaagtt ttcgaaagtt
    23821 gttaagtttt tttgcgaaaa ctatttttat atcatatata tgtctaaaat ctatattata
    23881 aaagaaatgt tgtttgatca gcttctcttc ggtattagtt caaaacaaaa cggcaaagta
    23941 tatggagcta ctttgccgtt ttgtttctct aatactcagg tcagtttata gacgctcaat
    24001 aaacggtgtt caaaagccag tttctcggta ttaagtcaaa tttatgcaaa aatgtgagaa
    24061 gccattttca aaaaatattt cttaatactc gaaactgagc ggctttttca cgacctctca
    24121 ataaacagtg ttcaagagac agcctatcgg caataaggct gaacgtctct ttcacaacct
    24181 cttttatcaa tctgctactt cttctttctt agcgaaactc caaatcagtc cgataacaaa
    24241 accgataact gctggtacga tccaccccat ccccaatttg aaaaacggca agtagctttc
    24301 cgcaacatat aaaagatttt gaacaatagg tgtttgtttg atagtttctg ggcaagcgtt
    24361 caacccatca aaaatcgaag caatcaatgt aaaataagta gtcattcgat aaacagccgt
    24421 acgctgctta aacaaaggac caactaacac tagtaaaatc agtgtcattg ctaatggata
    24481 gatgaacatc aaaacaggca acgaaaattg aatgatattc gtcaagccta cgtttgccgc
    24541 taaacaagga aggatactag caagtgcaac aaagaatgcg taatttcttt gcggaaacaa
    24601 atctgtgaat gtttctgcaa aagctgtaat caaaccaata ccggtcttca agcaagctaa
    24661 tataacgata aatgctaata agatactgcc ataagtacct aaataatgat cagcaatctg
    24721 agccagcgtg atcccgccat tttcacttag agcaaagcta cctaagctca tagcacctaa
    24781 ataagcgagc aaagtataaa tgattcccat caatataatg ctgatcgcac cagatttgat
    24841 cgtgtcttta gcgatttcag caggttttgt tacacccatg ttttggattg ttgtgacaat
    24901 gatgatccca aatgctaaag cagcaagcgc atctaatgtg ttatatcctt gagtaaaccc
    24961 tgtgaaaaaa gcatgctctt gataatttgg ctgaataggc gcttgattta cagaacctag
    25021 tggattcaaa aaagcaagga gaagcaaagc acctaataaa accaaaaatg caggattcag
    25081 gaactttcca acataatcta atatctttgt cggttttctt gaaaaccacc aagccagcac
    25141 aaaaaataca atactgaaaa ttgctagata tagcgtctgt ctgctaggat cgataaatgg
    25201 tgctaaacca atctcgaaag atgtggttgc aaggcgaggt aaagcaaaaa acggtccgat
    25261 aactaagtaa agcaagatgg taaaaaccat cgcatatgtt tgatttatcc gttttgctaa
    25321 ttcatataca cctgagcttt tcgatacacc aatggcaatc acccctaaga agggaagacc
    25381 gataccggta accaagaaac ctagattcgc ccaaaaggta gcagcgcctg cttcttgacc
    25441 caaatgaacg ggaaagataa gatttccagc accaaaaaat agaccgaaaa gcatggagcc
    25501 tataaaaaga tagtctttaa atgataattt ttttgtcata cataactcct ttacggtttt
    25561 tttaaaagta acaaaatttc gaaaaatatg caacaatttt cagaaaattt cctatataaa
    25621 acgacggtta tttaataacg taagtgataa aataaaagcg ctgaatcatc tgaggaggaa
    25681 tcatcgtgac aaagtcgaga ttagaagcat ttactgatgg agtggtagcc attgttttga
    25741 gagttcttgt attagagatc aagatcccta atacatctag ctttcattct ttatgggtca
    25801 tccgaaatac attattagct tataccatca gctttatatt tgttgcggtg atctgggtga
    25861 accatcatcg tatatttcag atggcagaac gaattaatta ccgagtggtc tggtcgaata
    25921 tctttttgct cttttggctg actttatgtc cttctgttac tagttgggtc gggcgtaacc
    25981 ctgaagcttt ttggcctgag atggcttatg tcttagtata cacgatgtgg agtttttctt
    26041 ttggtatctt gatgaggcaa atcatgaaag caaataaacc agattcccat gtcgtgagag
    26101 ttttgtcaaa ggaccgtcga agtcttatct ccatgatgat taacttatgc ttgattgccg
    26161 gtgtcttttt tgtaccgttg atcgggttat tcggacgttt ttttgtatct ggtatttgga
    26221 ttttttccta taaaaaagca gatgcctatt atcagcggtt gtttccgggg aaatagaaaa
    26281 gaggctggga cagaagcttt taactccaag aaataagccg aaatttcaca aaaatttaag
    26341 aagcaatttt cgtaaattcc ttcttatttc tcgaagctga ccacttctgt cacaactact
    26401 ttcttacttc ttacgttcat atttcatatg atagattagt aatagaagca agaaccacac
    26461 aggtgttagt aataaagcga ttctcgtatc ttttcctagg aacaagacga cacacacaaa
    26521 ggccatgtaa agaaatacca gataattact aaatgggtac aacggcattt taaatgaatg
    26581 agcttttttg ccgaggatac ttttacgata tttcatgtgc gtgtagacaa gcatactcca
    26641 gataaacaag aaacaagtag tggcaatact gcttactagt gtgaatactt cactaggcat
    26701 cacgtaattc aagataacag aaatgaaaat gacgatagta gaaaatgtca aagcattccc
    26761 tggcacatga tgtgttgtta actttttgaa ttttttaggg gcactgcctt cttgcgctaa
    26821 agaacgaagc atacgtcctg tactatatat tgcactgttg caggcagaag ctgctgcact
    26881 taatacgact aaattgatca aggaagcagc aatcgtgatc ccaatttccg aaaatacttc
    26941 tacaaatgga ctgttatctg gattcaagct gttccatgga tagattgcca taatgacaaa
    27001 aagagatcct aagtaaaata agatgatcct gataggaata ttattgatag cttccggcaa
    27061 cactttttca ggattttctg tttctccagc gactaatcca actaattcta tcccagcaaa
    27121 agcaaaggtt accatttgga aagctaagat aaatcctttt gcaccagttg gaaagaatcc
    27181 gccatgagac catagattcg tgatactggc tgtgccaata tctgtcgtat agtgggtgag
    27241 gatcatataa gctcctacga ggataaaggc aatgatcgct acaactttga tcaatgcaaa
    27301 ccaaaactct aactcaccaa acaatgagac agccaccata ttcaagcaaa gaagaagacc
    27361 taatgcaatc agttcaggaa gccactgagg aacaccaggc gcccagtagc gtacatacat
    27421 tccgatggct gttaaatcgg ccattgcaat cgatacccag cagaaccaat acgtccagcc
    27481 agtgatgaaa gccgcttttt tgccaagata ttctgcaaca aaatccagaa aagaatggtt
    27541 attggtgttt gataacagaa gttcgcctaa cgcacgcata atgaaaaaac agatgcatcc
    27601 agtaataaga taggcgagca atatcgaagg gccagctagc tggatagact ttccggaacc
    27661 taaaaacaac cccgttccaa tcgctccgcc aatagaaatc agttggacat gtctattttt
    27721 cagtccacgc tgcagttctt gctcttcatt catttttaaa tcatcctaac taaaaaagtt
    27781 taattacttc catattaaaa gagttttctg aaaaacacaa ctaagattcc aaaattttca
    27841 gaaatttacc ttttatgagt aattcataaa aaagaactgc ggtaaacacc gcagctcttt
    27901 gtcccaatca gctattgatc ctaagcattt aaaatacgtg aaaggaattc ttttgtacgt
    27961 tcttctttcg gatggacaaa gatatcttct ggtgtacctt cttctgcaat cacaccttta
    28021 tccataaaaa tgacacggtc agatacatct tttgcaaatt ccatctcatg cgtcacgata
    28081 agcattgtca agccagtatg tgctaagtct ttgatcgtat taagcacttc gccaaccatt
    28141 tcagggtcta acgccgaagt aggttcgtcg aatagcaata catctggatt cattgataat
    28201 gcccgagcaa ttgcgacacg ctgtttctgt ccaccagata attgtgctgg tttggcttcg
    28261 atataacgat ccataccaac tttttccaga ttttcgattg ctactttttt ggcagtttct
    28321 ttatctcgtt tcaggacagt tgtttgacca gacatacagt tttccaagac attcatattg
    28381 ttgaacaggt tgaaggattg gaaaaccata cctagattcg ttcgatattt aggtaaatta
    28441 tatttaggag ctaatacatt ttctccatta taaatgatct cgccgcctgt tggtttttcc
    28501 agtaaattga tacagcgcaa gaaagtagat ttaccagaac cggaagaacc gatgatcgtc
    28561 acaacttcac ctttattgac tgttacgctg atatctttca atacttcatt ttcaccgaag
    28621 ctttttctta gatgtttgat ttcaataatt ttttcattca tgattcttcc tccttattcc
    28681 tgcaaatttg aattgtcgat ttcttcaagc ttggtataag ctgaaggacc atcgattttc
    28741 ttttctaccg cccgcaggat tctggtgatc gtaaaggtca tgactaagta gatgatcccg
    28801 acgatcgtaa atgtttggaa gaattggaaa ttcgcaccgg aagcagagtt tccttggaag
    28861 aacaaatcag ctacgccgat tacacttaaa actgctgtat ctttgatatt gataacaaac
    28921 tcatttcctg ttgctggcaa gatattccgt aaaacttgtg gaataacaac ttttctcatc
    28981 gtttggccat gggtcatacc aatggcttgc gcagcttcga actgcccttc atctactgca
    29041 aaaatccccc cacgaacgat ctctgtcatg taagctccag tattgagaga gacaataaac
    29101 aaagcggcaa tcgttcggtt caatgaaatg ccaaaagcga gtgccagacc gtagaagatg
    29161 accattgctt gtaccatcat cggtgttccg cggaatactt cgacatagat agaaagaagt
    29221 gcatttccga ttttttggaa gaagcgagtc agtgcatttc ctgattcagg taccgaacgg
    29281 aagacaccaa tcagtaatcc aagtgtagta ccgacaactg tcccgatcaa tgcgatgaac
    29341 aaggtaagtc cagcacctcg taagaacata ttgccgtatt gtttccaaat cgtagtgaaa
    29401 tcagaaatga tgccaggttt ttcttcacta tcagttgcag caggctgatc tttgactgct
    29461 tggtccataa ttttggttcg ttcatcgtta gagatgccag aaagaatctg gtttactttt
    29521 tggatatccg ggtcgccttt acgcatacca acagaaatct gaacatcttc cgggtttgtc
    29581 tggaagccat ctgcaccgct gaattcaagc atttccaagt ctttgttcac actagttgcc
    29641 gttacacctt ccggacgttc gctgacataa ccatcgatca ttccagaagc taaagctgtt
    29701 ctcattgctg agaaattatc cattgcttgt tgtttctgca catctggtat ctgatcgatc
    29761 acagtatagt ggaatgtatt taactgagca gtaatctttg ctccagaaag atctgctaaa
    29821 ctttttgctt ttgcatattt gctatctttt tgaactacta ctactaattg tgattcgtaa
    29881 taaggatcgg taaaatcaat ttcttttcgt cgttcagcag tagggctcat tccggcgatg
    29941 atcgcgtcga tcttgcctga ttgaagggca ggagccagac catcccattt cgtttggacg
    30001 atgactaatt ttttccctaa accatctgct acttttttag cgatttgaac atcataaccg
    30061 ccggcgtaac tattttggcc ttgaattggc aaggcattat tctcatctgt ttgttgagac
    30121 cagttgaagg gtgcataccc agcctccata cctacgcgaa actcatcgct cggtgttttt
    30181 tcatctgcat ttgccagaga tcccacactg aatagtgaga agacaaaagc aaatagtacg
    30241 gcaactgaaa ccatattttt tttcattttt ttctcccttt ctgattatat gatacacgag
    30301 cggtctagat gaaatgcggt aaagctttat ctgaaaaaat aaaaagcgac cgatttatag
    30361 atataaaaag gccgctaaaa ttccataagg tgataaacct ctcacaaaac atagcgcaac
    30421 ttagcatcac tgctaagaca gtccgcaagc tcttaacttg cgtcccaaca tacactccaa
    30481 gcaaaaaagt gcacatttcg gcgatcctcc ctattcctgc ttctccatgt ttgcttcact
    30541 ccacagggtt aatgattctc gcgacctcta cctcattgat tgaggcatct cgtattcagt
    30601 tatttataca aaaaaagttt acgatactcg agaggctttg tcaatctata actgctgttt
    30661 tttgaaaaaa tactgataaa tatagaaatc tttactttta ccgaaaaaac aatcaaaatg
    30721 tgaatcatct atccattgca aacgatagta taattataca caaaaatgta tgtaatacaa
    30781 taaaaaaaga actagaacag aaaatttcct gtgtccagtt ccatctcgat catttaagct
    30841 gccgctttct ttggctcacg tcctaggatc gcttgcaaaa gaacaacgac gccataaatg
    30901 gctaatatta gataaatcaa acttgagtat tgggtgattc cccagcccca tgttcgatat
    30961 acggctaatg taatggctaa aacaattgcg gcaatcacgt tgaataaagc aaaagcccaa
    31021 agatttccat tttctttgcg tacaagataa aaaacagaga aataaacgct taacgctaac
    31081 caggcgatac aagccagaat aacgccccaa taaataaaga atggtaacat atcgattcac
    31141 ctcactttta tacagcagta ttgtaacacg ttttcattct tttgtcagtg aacattgtga
    31201 taaattgttc aaatgcccat aacacttttt tattcccatt tttctgactt ttcaacaata
    31261 aatttgttcg agtatgggct ataatggaaa gtgaggtgac aaaacatgaa acaattaaca
    31321 gtagacgaag cagcaaaaga gatattagct tatcagcaaa ttacattttt gaccggtgca
    31381 ggggtatcca caccttctgg agtgccagac tatcgatctt tgtcaggaat ctaccacggg
    31441 atggagcagc cggaatactt gctgagtcat caagcaatgg tgaatgaacc ggataaattc
    31501 tatcaatttg tcaaaaagat ttatcatcct gaagcaaaac ccaatatcat tcatttggaa
    31561 atggcacatc tagcgaaaga aaaaaatgtt tgggttgttt cccaaaacat cgacggtttg
    31621 cacgcaaagg ctaaaagccc gcaactcgtc aattttcatg gaagtttgta tcaatgtcat
    31681 tgtcgaaaat gtcaacaaac ggtagactgg aaagaatatc ttcacagcga caaacatcaa
    31741 gcatgcggag gacaaatacg cccagacatc gtgttatatg gtgaagggtt tcaagatgga
    31801 gtcatggagc aggcggctta tgccgtcagc caagcagagc tgatcgtcat tgttggaacg
    31861 agttttcaag ttcatccgtt ttgtgatctg gtacagtttc gccagccgtc agccaaaatc
    31921 ttagtaatca atcaaacgcc tgtttattta gcggagcctt atacgtttat tcaagaagac
    31981 ggagcggttg ttttcaaaaa aatacaagaa ttaggagcag attatgactg aattcaaaac
    32041 cgaaaaagaa aaaatgattg caggcgagct ttattttgcc aatgatcctg agctggttgc
    32101 cgaccgtatg tttgcaagaa gtcaaagcca aatcatcaac caagcagaat cagcggaact
    32161 gcgatcccaa ttgttgaaag agacttttgg ccgtacaggg aaaaaaatct atatggagcc
    32221 agttatcaat tttgattacg gatataatat atttgttggg gagaattttt atgcaaattt
    32281 caattgtaca tttttagatg tcagcacgat tgaaatcggt gataactgca tgttcgctcc
    32341 gaatgtccag ctttacacgg cgacgcatcc actacatcca gtgaaacgta acagcggtct
    32401 ggagtacgca aaacccatta aaatcggtaa taatgtttgg ttaggcggag gagttatcgt
    32461 cacgccgggt gtcactctag gggacaacgt agtagtcggc gcaggttcgg ttgtgaccaa
    32521 atcttttccc gacaatgtag tgattgcagg aaatcctgct cgtatcatca agacagtaga
    32581 ggaagaacta acagaagagt ctcttgaaac attacgtcat aagatcgata tgatcgatcg
    32641 agagttgatt gctttattag aaaaaagaat ggatacggta actaaaatag gacaagtgaa
    32701 aaaagaagaa aaccaagcag tttatgatgc aaaaagagag cagcaagtat tggataaagt
    32761 tgttagtctg ctgaaaaata agggatataa ggaaacaata acagatacgt atgttgattt
    32821 gatgaaacac tcgcgcgaat accaaaacaa aatgaaggag gaataagtgt gatcgaagga
    32881 gagacacggc gaaaacaaat catgcaaacc ttggcaaata cagaaaaacc agtgagtgct
    32941 agcaagtttg cgaaaacttt tggagtcagc cgtcagatca ttgtaggcga tattgcttta
    33001 ctacgagctg caggtaaaag tatcgtggca acagcaagag gttatatcct cgaaacagaa
    33061 gaaaccaata ctgggcacat cagtaaaatc gctgtccagc atggaaaaga tcaaacagaa
    33121 gaagaactga gactaatcgt tgaaaatggt ggtgaaatca ttgatgtaat tgtggaacat
    33181 cccctttacg gtgagttagc aggaactttg cacatcaaaa cgccacaaga tatcgattcc
    33241 tttatgagac gttatcaaaa aagtaatgct actttgttat ccgaattaac aggcggcatc
    33301 catctgcata cgatccgcta cccagaaaag catatgctga aaaacatcaa acaaaaacta
    33361 gctgaagcag gaatcctcta cgaaggataa catcgtagct gctacttttt atcctagaaa
    33421 ctattctatc atctctgaga taaacaccca tatggaagaa ataagaaaaa ataagaaaag
    33481 atgcgtatcc aatacacaga attttttgga attgcgattt ctagatgtct atctaaattt
    33541 cgattagtgg agaaacataa aaaagggaag tgcagctttt gagcactccc cttttttata
    33601 aagctttctt gtagcatagt atcgaaggaa cctgccaatt ctctactcga tcaccaatac
    33661 gccttctttt agtgcaaatg gattttgatc gttgatgtgg tcatagaaca tcacaccgtt
    33721 gatatggtcg atttcatgtt gaacgacgat cgattcataa ttcttcaaac gaatcttgtg
    33781 tttttcacca ttcatatcat aataagacac agtgattttg gcatggcgaa caacatatcc
    33841 tggtacttct cgatcaacag ataaacagcc ttcaccttcc cctaaacaag catcttgaac
    33901 agaatggctc aaaattttag gattatacat aacagtactt agagaaggtc catctgcctc
    33961 tgggtcagga cttggtacgt gaacagcaat gattctttta gaaatatcta attgaggagc
    34021 agctaagcca acaccgcctc gtaagtgtag ttcttcagct ttgattggat cttggctgtt
    34081 ttttaagaac tcaagcatct cttttcctaa agagatatct tcttcgctta atggaagaga
    34141 aacttctttt gcaacttcgc gtaaggtcgg atttccttca cgaatgatat catccattgt
    34201 aatcatggaa tatgtacccc tttcttaaaa gactgtgttt caagtctatt ttcgcattat
    34261 agtttaccac aaaaatggaa aagtagtagt aagattgttt gaaaactacc ttttcttaat
    34321 gattaactac ttgattttca acccggtttc aggtaaagta tagctgtgtg ctctaggcac
    34381 atgacctgcc acttggttaa gcgaatcacc aacgttttac tcagtcgcag gggaataatc
    34441 gaaagaggtg aaagaaatgg caatttcaaa agaacgcaag aatgaaatca tcaacgaata
    34501 cgctcgccat gaaggggata ctggttctcc agaagtacaa atcgctgtat taactgaaga
    34561 aatcaaccac ttgaatgaac atgcacgcgt gcacaaaaaa gaccatcatt cataccgcgg
    34621 cctaatgaaa aaagttggtc accgccgtaa cttgttagct tacttacgta aaactgacgt
    34681 tcaacgttac cgcgaattga tcaaacgttt aggcttgcgt cgttaatttt agtcaaaaag
    34741 agtgggattc acatgaatct cacttttttt actcgtatat caattaagta aaattccgtg
    34801 atcaagctag atggactcct actaaccaaa tgaaagctgc tcaaaagacg gctttcggca
    34861 gttttcattt gtttagtagc gtcttgcttt cacgagaagg aaaaggagaa gttatgtcag
    34921 aaaaacaagt attcaaaacg acatggggcg gccgtccctt acaaatcgaa gtcggtcagc
    34981 tagcaaaaca agcaaacgga gccgttttag tccgttatgg agatacagta gtcttaagtg
    35041 cagctgtggc ctctaaagaa gcaaaagata cagacttttt cccattaacg atcaattatg
    35101 aagaaaaaat gtatgcagca gggaaaatcc ctggaggttt catcaaacga gaaggccgtc
    35161 caagtacgga agcaacattg actgcccgtt tgatcgaccg tccaattcgc ccgatgtttg
    35221 cagaaggctt ccgtaatgaa gtccaagtaa cgaacatcgt gatgagtgta gaaacagact
    35281 gctcgccagc tatggcagca atgctaggtt cttctttagc actatcaatc tcagatatcc
    35341 catttgacgg cccaatcgca ggcgtagaag ttggtcgtgt aaatggcgag tacgtcttga
    35401 atccaacagt agaacaagca gaacaaacag atatcgaatt gactgttgcc ggaacgaaac
    35461 aagccatcaa tatggttgaa agcggcgcga aagaagtttc agaagaagac atgcttggtg
    35521 cattgctgtt tggttttgac gctatcaaag agttagttgc tttccaagaa gaaatcgttc
    35581 aagcagtagg gaaagaaaaa atggaagtga ctttactgca agttgatgaa gtattgaaaa
    35641 aagaaatctt tgatgcttct tacgctacaa tgaaagcagc agtaatgact gaagaaaaac
    35701 ttgctcgcga agataatatc gagcaggtga agatcgatat ccgcgaagct tatgctgaga
    35761 aatttgcagg tcatgaagat gaagatcact tgttgaaaga agttaaacaa atcacagaag
    35821 atcttgaaaa agatgtcgta cgtgaattga tcaccatcga taagatccgt cctgacgggc
    35881 gtaaattgga tgaaatccgt ccattgtcat cagaggtcag cttattgcca cgtgtacatg
    35941 gatcaggttt gttcacacga ggacaaacac aagctttatc agcttgtaca ctcgcacctc
    36001 taggagagca ccaaatcatt gatggtttag gggtggaagt cagcaaacga ttcatccacc
    36061 actacaattt ccctcaattt tctgttggtt ctacaggacg tgcaggctct ccagggcgtc
    36121 gggaaatcgg acatggcgca ttaggtgaac gtgcgttagc acaagtaatc ccttcagaag
    36181 aagaatttcc ttatacgatc cgtttagtag cagaagtatt ggaatcaaat ggttcttctt
    36241 cccaagcaag tatctgtgct ggcacattag cattgatgga tgcaggtgta ccaatcaaag
    36301 cgccagtcgc cggtattgcg atgggacttg tttctgatgg tgaaaactat acgatcctaa
    36361 ctgatatcca agggctggaa gatcatcttg gcgatatgga ctttaaagtt gccggtacaa
    36421 aagatggtat cactgcttta cagatggata tcaagattca aggaatcaca gaacaaatcc
    36481 taactgaagc attaactcaa gcaaaacaag cacgtatgga aatcttagaa gaattgactt
    36541 caacgattgc tgctccacgt gaagaattaa gccaatatgc accaaaaatc gaaatgatcc
    36601 agatcgaacc tgctaaaatc aaagatgtta tcggtaaagg cggcgatacg atcaacggaa
    36661 tcatcgatga aactggtgtg aaaatcgata tcgatcaaga tggaaaagta agtattgctt
    36721 cagctgatgc agaaatgatc aaaaaagcta tcaagatcat cgaagatttg accaaagaag
    36781 tcaaagttgg tgaagtttat ctagggaaag ttgtccgtat cgaaaaattc ggtgcattcg
    36841 tcaacttgat caaaggaaaa gatggacttg tccatatttc ccaattagca aacgatcgtg
    36901 tcaataaagt cgaagatgtt gttaaactag gtgatgagat ccttgtgaaa gtaactgaaa
    36961 tcgataaaca aggacgcgta aatctgtcaa gaaaagcaat gttgaacgaa gatggttcaa
    37021 cgaaaaatga agaaaaataa tttaattcat aggaataatg tacttgtgat cgtgaaggtg
    37081 acagggggac aataattatt cgaaaaaagg ggactgtgac aaaagccgtg tcacagtcct
    37141 tttttttcga ataaacggtg ttcaaaagct tgcatctgcg gtaataagtc cagccaagtt
    37201 aaaaacatta ggagctgttt ttatttggct gttcttatta cttgatgcag aacagctttt
    37261 ttacaacctc tccaataaac gatgttcaat cagctgtccc tcgatattag ctcaaaaaac
    37321 ggcaaaatat gaggaactat ttttccgttt gtttctctaa tactcaggtc aaaccgctga
    37381 tttcacaacc tctgattcac ggtgttccag aagcagcttc tacggaaata agctgaacac
    37441 ttttgttaca acttcttttt ttctggagtt tcttggcgca tatttttgga gtccttctga
    37501 ataaagcgta aaataaaagt gagaaatatg gataaaggag ggaaaggaga tgctgaattt
    37561 tttatggtcg ttatttatcg gcggtatatt aggtttgata gctggcgcaa ttttaggaaa
    37621 ggaagtcccg ggaggcgtat ctggaaatat cattatcggt tttttaggta gttggctcgg
    37681 cgaattttta ctaggaccac tgggaccgga actcggcggt ttttatttga taccagcatt
    37741 attgggtgca attttttgcc ttgctcttta ttcatttgtg gcaagacgta tcagagaacg
    37801 tccaaagaaa gaataatttt attcaaataa gttgacactt cagtgtgtta cttggataat
    37861 tagaagcaat ttaggaaagc gggtgtcatg gataagaata tcatctatca ttcaagctta
    37921 cctaaaggat atccatataa caagaaatga gggaaaaacg tggcaacaac gcacaatacc
    37981 aaaaagatat caatgactgg cctgcttgta gcgatgggag tcgtctacgg agacatcggg
    38041 acaagtccgc tttacgttat gaaatcgatt gttgaagata atggcggatt gtcaggaatc
    38101 aatcctgatt ttgttatcgg atcagtctca ttgatttttt ggacattgac tctactgaca
    38161 acaatcaaat atgtcgtgat tgcattgaat gcagataacc atggtgaagg tggaattttc
    38221 tcactctaca cacttgtccg aaaaggtggg aaatatttga tcattcctgc aatgatcgga
    38281 ggagctgcct tactagcaga tggggtattg acccctgctg tgacggtaac gactgcgatt
    38341 gaaggcttgc gaggaattcc gcagtttttt gaccggttcg gtaataacca gaacgtgatc
    38401 gtcattatca ctttagttat tatttttgtt ttattcatgg tccaacgctt tggtacagat
    38461 attgtcggaa aagcattcgg accaatcatg ttcgcttggt ttactttttt aggcgtaatg
    38521 ggattaatga acttcagtca agactggaca gttatccgtg cactgaatcc ttattatgct
    38581 atccagctgc tttttagtgc agataacaaa ttaggcttgt tcattcttgg aaatattttc
    38641 ctggctacga caggtgcaga ggcgctttat tccgatcttg gacatgtcgg caaacgaaat
    38701 attcgtctga gctggcctta cgtcaaaatt tgtttggtta tcaattattt tgggcaggct
    38761 gcgtggttgt tgaatgttta tcaaaaccct gaagcacagc aaatcaagaa cctgaatcca
    38821 ttcttccaaa tgatgcctca aagttggatc gtgatcggcg ttgcttttgc aacagtcgct
    38881 gcagtcattg cttcgcaagc attgattaca ggttcgttta cgctagtttc agaagcaatc
    38941 aagttgaaac tgctgcctcg gttaaagatc atgtatcctg ggaacagtat cgggcaaatg
    39001 tatatcccag cagtcaatct gattttatgg atcgcctgtt ctctagtggt tgtcacattc
    39061 cgaacgtctc atcatatgga agctgcttac ggtctatcga tcactgtgac gatgttgatg
    39121 acaacagcac tgctttactt ttatcttatc caaaatgggc attctcgttg gcttgcatat
    39181 ttagttacct ttttcttcgg tgcgatagaa gtcgttttct ttatctcaag tgttgtcaaa
    39241 ttcttccgag gcggttttgt agccgttctg attggtttga tcatcttagt cgtgatgttt
    39301 atttgggaac aagggaacat tattcgcgaa tcgtcaaccg aggatgtgtc tttgagagat
    39361 tatatcccgc agcttagaga attacgtgat gatacttctt taccaatcta tcagacaaat
    39421 gtggtcttct tggttcctga tatggctgaa gataaagttg gccgacagtt catctattcg
    39481 attttagata aacggcctaa aagagcacga gtctattggt ttgttcatgt ggaagtgacg
    39541 gatgagccat atacgaaaga atatcaagta gatatgatgg acactgattt tattgtccaa
    39601 gtgaatctgt tcttaggttt cagggtccaa caggaaatca acgtatatgt tcgccaagtc
    39661 atccatgatt tgatgaaaca aggccgtttg ccaaaacagc cacaaagata ctctctgaca
    39721 ccagggcgag aagttggcga tttccaattt gtcctgattg atgaagtagt ttccaatgtg
    39781 acaactctag gaaaatggca acgtcaaatc atgcaggcaa aactggcaat caaaaaaatc
    39841 gctacctcgc cagaaacatg gtttgggcta gagtacagcg aagtaaaaca tgaaactgtt
    39901 ccgcttatca ttggaaatac tcggaaaaca tggttgaaag agcggttgtg aaagtagcgc
    39961 tttgacctga actaatgtcg aatgtcagct acttgaacac cgtttatcag aggtcatgaa
    40021 aaagctgtac tgcaacaagc agtaagaacg agcagataaa aacagcttct catgtttttc
    40081 atctgtttgg gcttaatgcc gtagatgcaa gcttttgaat accgttttta tcagaggtca
    40141 tgaaaaagct gtttagcaac aagtaacgca atgattttga agtgataagg agatgtggct
    40201 gaaaaatgcc gcatctcctt ttttattcct aaatatgaat acgcattatt ttcaatcttt
    40261 ttgtagaaaa agggtacaat agaaaaagag aaaggcggga gaatatggtt atcaatttca
    40321 ttattttatt tttgatgatt gcaattactt cattttttgt agcaagtgaa tttgctttag
    40381 tgaaaatcag acgatcaaga ttggaacaac tagaaaagga aaatgtaaag aacgccaagc
    40441 tagctcttca tgttacacac catttagata actatttatc agctagtcaa ttaggaatta
    40501 cgctaaccgg tttgatcatc gggtgggtcg gtgaaggatc tgtcgcggct ttactagaac
    40561 cagtgatcgg taaactacct tttagttcgg ctattagcag tacaatctct gtggcgctgg
    40621 gatttatttt agttacgtat atcgatgtgg ttattggcga actgcttcct aagagctata
    40681 gtatcgtcaa tacggagaag gtcgtcttgt tcgtagttaa accacttcat tatttttata
    40741 aagtaatgtt tccttttatt tgggtattga atcattcagc tgcagggcta gggaagctat
    40801 taggtgtgcg tctcgtatct gaaggagaag agacattgtc tcaagaagag ctgacattag
    40861 tcgcattgaa ttcttatgaa aaaggagagt tgaccaaaga agaatattat tatttggaaa
    40921 acgtgtttga atttgatgat accttggcaa aagatataca agtagaccgt acgtccatgc
    40981 aagtattcga agcttcagaa accgtcaaag aagcgataaa agcttcgatt gaacaaggac
    41041 atactcgcta tccggttatc ttagaatcaa aagacaatgt tttaggttat gtcaccttgc
    41101 cagatttgat caagcaatct tataaagatg accagctgac agtagaacag ttgattgaag
    41161 agccgatcgt aacaactgaa acgatcccta taaaaaaatt attgacgatc atgcgaaaaa
    41221 agggtaagca tattgctatc ttaaaagatg aatacggagg gacaagcggc ttagtgacga
    41281 ttgaagatat tttagaagaa atcgtcgggg aaatccgaga tgagacagat ttggacgaag
    41341 cattgatagc ggaacagtca gatggctctt atatcatctc cggcaaacta acattagatg
    41401 atttccagcg ttattttcat gtagagattc cagagtttga agaaacgaat tttactacac
    41461 ttgcgggctt tgcttctagc cgatataaag aaataaaagc aggaacaatc attgagattg
    41521 catcattccg tttcacggta ttagaatacc agcatgcaca tatcgattat ttcaaagtag
    41581 agtccacgga aagaaaaaca gaataaaaga aaaaaggctg tgacattttt tgtcacagcc
    41641 tttttgttat ctaaaaaacc tgttttcggt aaacgaagaa gatattttca ataaaagttt
    41701 tcatttttag ccgcaacgat ttcttttaat caaggctatc ttgcttctgc atgttctaat
    41761 gtgaaatgtt cctcttcacc catgtgtttc aattgtttca tcgaacgcat ataagagtaa
    41821 agcaacacgg caacagaacc gatccaagct aaaggagaag ctgctgctgc tcctgggtat
    41881 ccccacattc ctgtaagaac gatagctgca aaagaacgca taagcaattc catcataccg
    41941 gcgatcgtag ggatcttgct ttgtccaagc ccttgaagtg tgtagcgtaa aatgaacagg
    42001 actgctaaga tccagtaaag agacccattg atattgaagt aggtttgtgc taattcaaat
    42061 acacgtgttt ctgatgggtt aacaaacaat tgaacaaaga aatgaccgaa gaaaatgacg
    42121 atagcacctg ctaagagact aaatccgaca ctcatcagta aagtttgacg taccccttgc
    42181 aagattcggc catattcttt cgcaccatag ttttgtgcag tgaaagttgc cattgcaata
    42241 ccaaatgaca tcatcggctg atttgcaaat tggtcgatac gaccagcagc ggcttgagca
    42301 gctacgacat ctgtccctag actatttagt gctgcttgta acacgatagc accaacagcg
    42361 atgatcgaag attgaaacgc cattggcaaa gcagcattta ggtgtacagc aaactcttct
    42421 ttttcaaatt tgaaatcttt tttgcgtaac tgtaacaaag gcattttttt cttgatatac
    42481 actagacaca gtacgctgga agaaacttgt gcgattactg tagcaatagc tgcaccttcc
    42541 acacccatgc caaaatagat aataaagatc aaatcaagta cgacgttgat gatgaccgca
    42601 atgatcagaa agaatagtgg tgtgcggctg tcaccaagtg cgcgaatgac attagaaagc
    42661 aaattgaaac tgactgatgc aaagatccct aataaaataa ttgaaataaa tgtttgtgct
    42721 tgatcaagta tttcaggagg agtttgcatc aattgaagca tcggacgaat aaacaggaga
    42781 cttaggacag ttaaaatcac agtgacgaca agactaatga caacactagc agcaaaactt
    42841 ttcttcagac cccgataatc ttttgcaccg taacgttgtg cggtaataat ggctaaacca
    42901 gctgttaaac cttgggcaaa gccaatgatc aaaaatgtca ggcttccagt tgcaccaacc
    42961 gctgccagcg catttttacc tagcgtctga cccacgatga tcatatcgat catattgtag
    43021 aactgctgaa aaacatttcc gatcagtaaa ggaacagtga acatcaaaat cagtttagcg
    43081 gggcttcctt ttgttaaatc gcgcatcttt taaactcctt atatctttaa aatttagtta
    43141 gtagtatata gcgtttatcc tgagaatacc aattagaaat caatgataat ttatgaaact
    43201 ccataaaaag aagccaagaa ttgagtcaaa gaatcaacaa cttggcctgg tctgtaaaat
    43261 aagggacatc cacgtttatc tatcacctta atattgtcta atctcacttg gtaaaaggat
    43321 cgtttctctc gtaccgtcat cataggcgta tacttgagca ggaaattggt tcgaataggc
    43381 aaacggcaaa tcaatatcca tttccacatt gcttcctgct tgagaaaaat gcatcgtcac
    43441 ttttccacga ttttcgatca aatcaaaggt taatatattt tctaatggaa tgactccctt
    43501 aagatctagg tcaataataa accatatact gtcaatcaat tcatctggca aacttgatac
    43561 gacaccaact gaagcgtaac ggttgcggct gctatcaaaa ctttcaaaca tctgtttaac
    43621 ctccttcgaa tcaacgaatg ttttattttt ataattaatc ctaggataaa ctagaaatac
    43681 ttacttcaac gagggttcat tctaataaag aatcaggaaa aatgcaacaa taattgaata
    43741 ctttttttca aagtataaat ttttataaag gtacgataaa agtatgcgag gaaaagcttg
    43801 gtaaattagg atttttcgtt tgtaaagcca tttacaaaag tggaagcccc atatgaaaat
    43861 aaacgattag aaataaaaaa ggcgtgttaa aagctgctga cccgccgctt ctaacaccac
    43921 ctgattgttt cagtattatt cttctgttga accgatcact tctggttcgc caccttcacc
    43981 tgtgctttct tcttcttcac tttgagcgac tacagcagcg atttgctctt cactgtcggt
    44041 cacgaatttg taatcgtcgt tttttggaag atcggctact gtaatagaat cacctaattc
    44101 caagtttgtc acgtcaactt caattcgttc aggcaatttg tctggtgtag cagatacgac
    44161 tactgtatac aagttttgag ctaacactcc accagattta gcacccactg attcgccaac
    44221 aagaacgact tctgtttcga cttctgtttc ttccgacatg tttacagata gaaattctgc
    44281 atgttcgaaa tgattggtaa aagtgttcgt ttggatcttg tgaaccaatg tattgacttt
    44341 ttgtccatcg atgtccatcg tgatgactgt gttcgctcca tgttcacgca acaatttgct
    44401 tagtgtcaaa ccatcgaatg agataggggt actttctact ttatacccat aaacaacagc
    44461 tggaactttc ccttcgtgac gaagtttgtt tcttaatgaa cggggacgta cttctctttt
    44521 acttacttct aatgatactg acataaccaa aattcctcct taaaatgtga ctgtctgctt
    44581 tggtaacgga cagattttat ttaagtgaat tctaaaggct aatctcgtgc acatcatcga
    44641 aggcagggcg cttaaaaaag cgcaaaagga gagatgcgcg catctcccct tagaaagtca
    44701 ggattcaact tttctccacc aggtcattac gctgtgtatg agaaatccaa ttcactttac
    44761 tatttaaccg taacgtgaac tgttaattaa gtcaaggaat aactatgatg atgtatttgc
    44821 aagcaagaat attcaaggta taattaatta gtagtaataa gaaaggaaac agcatgcgtt
    44881 tagataaatt aatagaagaa aaattagaaa cgacacgcaa ggaaatgaag cggctgtttg
    44941 ctttgaagca agtgatgata gatggcacag tcgaatttcg acaaaatcga aatgtagata
    45001 gtttactgca tcaaatcgaa gtagcaggtg aacacttaga aacaaatgag tcctattatc
    45061 ttctcaataa acctgaaaaa gctgttacag ctgtatcaga tccagaaaag acgacagtaa
    45121 tcgatctgat tgcaagtgcc gatcgtttcc aaccattata tccagtggga agactagata
    45181 gagatacaac aggactattg ctgatcactt cgaatggaca attgggatat gaaatgctac
    45241 ttccagaaaa aaaggtttca aagacttatc aagtagttgt gaacgaaagg gtgacaccgg
    45301 cagacgtaac agcatttgaa aatggaatcg tattcgctga tgggtatcaa tgtaagccgg
    45361 ctgatttaaa aatactcaaa gcagacaagc accaaagtac ggttttattg acgatccaag
    45421 aaggaaaatt ccatcaagtc aaaaagatgt tcttagccca aggaaagaaa gtgaccgcat
    45481 tgaaacggct aagcatggga ccattggaat tagatgaaca gctaggagta ggcgagtatc
    45541 gatcgttgac attagcagag atgaaaaagt taagtattta ttttaaatag gaggtcaaaa
    45601 agatgaaata taaaacttta ttgtttgatg tagatgatac attgttagat tttcaattaa
    45661 ctgaagccaa tgctttaagt gctttgtttg cagaacaagg aatcacattg acaccagaaa
    45721 tcgaaaccag ctataaggct ttgaatcatc atttatggca ggagtttgaa aaaggcagac
    45781 tatcacgaga agaaataata ggcagtcgtt ttgggttact tttccagcaa tttgggaaaa
    45841 cagtagatag tgatcaaatg gaaaaacgct accgccacta cttgaaccaa ggtcatgatc
    45901 ttgttccagg aagtctagaa atcctgaaaa aggtccatcc gcatgctgat ctttatattg
    45961 tgacgaacgg tgtgtcgcac acgcaacatc ggcgattaac agatgcagga atgcttgatt
    46021 acttcaagga tatctttgtc tcagaagctg tcggagcgca aaagccgatg aaagaatttt
    46081 ttgatcatgt cttttcccac attcctaatt ttgataaaaa gcaaactgtg atcattggtg
    46141 attctttgac ttctgatatc aaaggaggaa atatggcagg gatcgatacg atttggttca
    46201 atagaaataa attaccagaa atcgaagaag tccagcctac ttatcgaatc gattcattag
    46261 aggaacttta ttcattattg gaaattgaaa aggattaatt ttcagaaaaa tatagcattt
    46321 tcgtgttttt tcgctataat agattagaat gaaaaaacgg atacgaaaga ggttttagtc
    46381 aatgacaaaa agcaagccaa ttatcattgg tgtgacgggt ggatccggaa gcggaaagac
    46441 aagtgtcagc cgtgcgattt tcaacaactt cccggaccat tccatcatga tgcttgaaca
    46501 agattcttat tacaaggatc agagccattt gagttttgaa gaacgattga acacaaatta
    46561 cgatcaccca tttgcctttg ataatgatct gctgattcag catgtaggcg acttactgaa
    46621 ctataaagcc attgaaaaac ccgtgtatga ctatgtagcg catacaagaa gtcaggcaac
    46681 gatcattcag gaaccaaaag aagtgatcat tctagaagga atcctgattt tagaagacga
    46741 acgtctgcgt gacttgatgg atatcaaagt atacgtagat acagatgatg acattcgtat
    46801 cattcgtcgt atcaaacggg atatggaaga acggggacgc acattggact ccgtgatcga
    46861 acaatatttg acagttgtta aaccgatgta tcatcaattt atcgaaccaa cgaagcgcta
    46921 tgcggatatc attgttccag aaggcggaga aaaccacgtt gctattgatt tgattacaac
    46981 aaaagtagct agtttcttaa accataaata gtgcgcagcg aaacagttga acggactgcg
    47041 accgtttgaa aagcaaggat tctgcttttt gaacagtcac cagccaactg tttttttatt
    47101 tataacaaga ggttgtgaaa acagcatctt gacctaagta ttaaaaagaa gaaacggaaa
    47161 atagctgttt atattttacc gttttttgag ctaataccga aggtcagctg attgagcacc
    47221 gtgaagtaga ggttgtaaaa aagctgtctt tgcatcaagc gataagagca tccaaataaa
    47281 acagctcctc acgtttttcg ttgggttggg cttattatcg tagatgcaag cttttgaaca
    47341 ccgtttaacg agaggttgta acagaagtgg tcagatgcaa taaataagaa gaaacttacg
    47401 aaaattattt ctcgaatttg tgtgagattt cgacttattt ttttgaagaa gctcttttgt
    47461 cgcaccccc
//
LOCUS       DAZKKW010000013        45752 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.013, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000013 DAZKKW010000000
VERSION     DAZKKW010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 45752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45752
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.013"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(204..1700)
                     /gene="lysS"
                     /locus_tag="ACG8PO_000707"
     CDS             complement(204..1700)
                     /gene="lysS"
                     /locus_tag="ACG8PO_000707"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFQ0206058.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKAGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1811..2815)
                     /gene="dusB"
                     /locus_tag="ACG8PO_000708"
     CDS             complement(1811..2815)
                     /gene="dusB"
                     /locus_tag="ACG8PO_000708"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFQ0206059.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2816..3703)
                     /gene="hslO"
                     /locus_tag="ACG8PO_000709"
     CDS             complement(2816..3703)
                     /gene="hslO"
                     /locus_tag="ACG8PO_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFQ0206060.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3920..6031)
                     /gene="ftsH"
                     /locus_tag="ACG8PO_000710"
     CDS             complement(3920..6031)
                     /gene="ftsH"
                     /locus_tag="ACG8PO_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFQ0206061.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(6121..6666)
                     /gene="hpt"
                     /locus_tag="ACG8PO_000711"
     CDS             complement(6121..6666)
                     /gene="hpt"
                     /locus_tag="ACG8PO_000711"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HFQ0206062.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6666..8111)
                     /gene="tilS"
                     /locus_tag="ACG8PO_000712"
     CDS             complement(6666..8111)
                     /gene="tilS"
                     /locus_tag="ACG8PO_000712"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFQ0206063.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8231..8701)
                     /locus_tag="ACG8PO_000713"
     CDS             complement(8231..8701)
                     /locus_tag="ACG8PO_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 domain-containing RNA-binding protein"
                     /protein_id="HFQ0206064.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8747..9172)
                     /locus_tag="ACG8PO_000714"
     CDS             complement(8747..9172)
                     /locus_tag="ACG8PO_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HFQ0206065.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9239..9508)
                     /locus_tag="ACG8PO_000715"
     CDS             complement(9239..9508)
                     /locus_tag="ACG8PO_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HFQ0206066.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9519..11114)
                     /locus_tag="ACG8PO_000716"
     CDS             complement(9519..11114)
                     /locus_tag="ACG8PO_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFQ0206067.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(11129..14650)
                     /gene="mfd"
                     /locus_tag="ACG8PO_000717"
     CDS             complement(11129..14650)
                     /gene="mfd"
                     /locus_tag="ACG8PO_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFQ0206068.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14667..15227)
                     /gene="pth"
                     /locus_tag="ACG8PO_000718"
     CDS             complement(14667..15227)
                     /gene="pth"
                     /locus_tag="ACG8PO_000718"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFQ0206069.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15580..16554
                     /locus_tag="ACG8PO_000719"
     CDS             15580..16554
                     /locus_tag="ACG8PO_000719"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFQ0206070.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            16724..18124
                     /locus_tag="ACG8PO_000720"
     CDS             16724..18124
                     /locus_tag="ACG8PO_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HFQ0206071.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18202..18924)
                     /locus_tag="ACG8PO_000721"
     CDS             complement(18202..18924)
                     /locus_tag="ACG8PO_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0206072.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(19003..19509)
                     /locus_tag="ACG8PO_000722"
     CDS             complement(19003..19509)
                     /locus_tag="ACG8PO_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFQ0206073.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSINHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19632..21431
                     /locus_tag="ACG8PO_000723"
     CDS             19632..21431
                     /locus_tag="ACG8PO_000723"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HFQ0206074.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            complement(21499..23073)
                     /locus_tag="ACG8PO_000724"
     CDS             complement(21499..23073)
                     /locus_tag="ACG8PO_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFQ0206075.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
     gene            complement(23328..23837)
                     /gene="mreD"
                     /locus_tag="ACG8PO_000725"
     CDS             complement(23328..23837)
                     /gene="mreD"
                     /locus_tag="ACG8PO_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HFQ0206076.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            complement(23841..24695)
                     /gene="mreC"
                     /locus_tag="ACG8PO_000726"
     CDS             complement(23841..24695)
                     /gene="mreC"
                     /locus_tag="ACG8PO_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HFQ0206077.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            complement(24850..25488)
                     /locus_tag="ACG8PO_000727"
     CDS             complement(24850..25488)
                     /locus_tag="ACG8PO_000727"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HFQ0206078.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            complement(25489..26139)
                     /gene="rpe"
                     /locus_tag="ACG8PO_000728"
     CDS             complement(25489..26139)
                     /gene="rpe"
                     /locus_tag="ACG8PO_000728"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFQ0206079.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGRFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            complement(26152..27051)
                     /gene="rsgA"
                     /locus_tag="ACG8PO_000729"
     CDS             complement(26152..27051)
                     /gene="rsgA"
                     /locus_tag="ACG8PO_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HFQ0206080.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            complement(27216..29285)
                     /gene="pknB"
                     /locus_tag="ACG8PO_000730"
     CDS             complement(27216..29285)
                     /gene="pknB"
                     /locus_tag="ACG8PO_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HFQ0206081.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            complement(29282..30022)
                     /locus_tag="ACG8PO_000731"
     CDS             complement(29282..30022)
                     /locus_tag="ACG8PO_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HFQ0206082.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            complement(30035..31393)
                     /gene="rsmB"
                     /locus_tag="ACG8PO_000732"
     CDS             complement(30035..31393)
                     /gene="rsmB"
                     /locus_tag="ACG8PO_000732"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFQ0206083.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKMLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            complement(31393..32331)
                     /gene="fmt"
                     /locus_tag="ACG8PO_000733"
     CDS             complement(31393..32331)
                     /gene="fmt"
                     /locus_tag="ACG8PO_000733"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFQ0206084.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKKAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            complement(32324..32815)
                     /gene="def"
                     /locus_tag="ACG8PO_000734"
     CDS             complement(32324..32815)
                     /gene="def"
                     /locus_tag="ACG8PO_000734"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFQ0206085.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            complement(32830..35238)
                     /gene="priA"
                     /locus_tag="ACG8PO_000735"
     CDS             complement(32830..35238)
                     /gene="priA"
                     /locus_tag="ACG8PO_000735"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFQ0206086.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            complement(35491..35796)
                     /gene="rpoZ"
                     /locus_tag="ACG8PO_000736"
     CDS             complement(35491..35796)
                     /gene="rpoZ"
                     /locus_tag="ACG8PO_000736"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFQ0206087.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            complement(35801..36415)
                     /gene="gmk"
                     /locus_tag="ACG8PO_000737"
     CDS             complement(35801..36415)
                     /gene="gmk"
                     /locus_tag="ACG8PO_000737"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFQ0206088.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEKRMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            complement(36902..37186)
                     /locus_tag="ACG8PO_000738"
     CDS             complement(36902..37186)
                     /locus_tag="ACG8PO_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0206089.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            complement(37204..38094)
                     /locus_tag="ACG8PO_000739"
     CDS             complement(37204..38094)
                     /locus_tag="ACG8PO_000739"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HFQ0206090.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFVLDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            complement(38114..38332)
                     /locus_tag="ACG8PO_000740"
     CDS             complement(38114..38332)
                     /locus_tag="ACG8PO_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HFQ0206091.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            complement(38302..40002)
                     /locus_tag="ACG8PO_000741"
     CDS             complement(38302..40002)
                     /locus_tag="ACG8PO_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HFQ0206092.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            40269..41042
                     /locus_tag="ACG8PO_000742"
     CDS             40269..41042
                     /locus_tag="ACG8PO_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0206093.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            complement(41123..41692)
                     /gene="thiT"
                     /locus_tag="ACG8PO_000743"
     CDS             complement(41123..41692)
                     /gene="thiT"
                     /locus_tag="ACG8PO_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015234 - thiamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HFQ0206094.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     regulatory      complement(41758..41860)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(41866..42837)
                     /locus_tag="ACG8PO_000744"
     CDS             complement(41866..42837)
                     /locus_tag="ACG8PO_000744"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFQ0206095.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     gene            complement(42931..44304)
                     /gene="glmU"
                     /locus_tag="ACG8PO_000745"
     CDS             complement(42931..44304)
                     /gene="glmU"
                     /locus_tag="ACG8PO_000745"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFQ0206096.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            complement(44807..45625)
                     /gene="purR"
                     /locus_tag="ACG8PO_000746"
     CDS             complement(44807..45625)
                     /gene="purR"
                     /locus_tag="ACG8PO_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0045982 - negative regulation of purine
                     nucleobase metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HFQ0206097.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
BASE COUNT        12469 a         9909 c         8266 g        15108 t
ORIGIN      
        1 tttagcttgg tcaacgaatt ttttgtattt tttcaagttt ttttcaataa acgattattc
       61 ataacttttc tttctttatc gttcgttttc gtcttcttta atgtctagaa tagtcggcat
      121 tagcttttga actttcgtta ttatttattc ctttctacac agcaaaagct gcctaaggaa
      181 ttccttagac agcttttatt cgattatctc attgttggga atagcaaaac atcccgaatt
      241 gattgtgcat cagttagcaa cattactaat ctgtcgatcc cgatgcctag accaccagtt
      301 ggcggcatac cgtattctaa ggcttccaag aagtcttcgt ctacaccgtg agcttcgtca
      361 tttcctagtt cacgttcttt ttcttgcgct tcgaatcttt ctctttgatc aattggatcg
      421 ttcagttctg taaaggcatt agcaaattcg cgccccacta tgaatagttc gaatcgatct
      481 gtgaatcgag gatcttctgg atttttcttc gctaaaggcg aaacttcgac tggatgtccg
      541 tatacaaacg tcggttgtgt caatgtttct tcaacaaatg tttcaaagaa ttcattgata
      601 acatgtccga cacccatagc ttcggttact tctacattgt gtttttcggc taaagctaat
      661 gcttcttcca atgtcatttc ttgccagaag tctacaccag tttgttcttt gattgcatct
      721 accatatgga tgcgtttaaa gtcactttct aagtcgacag cttgtccatc atatgtgatt
      781 tttgctgttc ctagtacttt ttcagctgcg ttacggataa taccttctgt cagattcatt
      841 acatcattga aatcagtata tgctgtgtat gcttctaaca tcgtaaattc tggattatgt
      901 gtagtatcaa ttccttcatt acggaaaacg cggccgattt cataaacttt ttccattccg
      961 ccaacaatta gtcgttttaa atgaagttct agcgcaatac gcaaatataa atcaatgtcc
     1021 agtgcattat gatgcgtgat aaatgggcgt gcggcagctc cacctgcctc attgtgcaat
     1081 actggtgttt ctacttcaat atagccattg ccatctaaat agcgacggat ttcgctgata
     1141 atttggcttc gtttcataaa tcggtcaaag ctttcgcgat tactgattaa atccaagtag
     1201 cgttgtctgt agcgttgttc aatattcgtt aagccatggt atttatctgg cagtggacga
     1261 agtgcttttg acaaaatcgt gatttctgaa gctttgatag aaacttcgcc tgtgtctgtt
     1321 ttcatgattt gtccagttac accaaagaaa tctccaaggt ctgcatgttt gaatacttca
     1381 taggcctcat cacctacttg gtcttttctt acataaattt gaatttggcc ttcacgatct
     1441 tgtagatgcg caaatccagc tttccctttt cctcggcgag tcatcatacg accagctaca
     1501 ctagcggtca atcccatttc tgctagttct tcttttgttc ttggatcaaa tagttcatgc
     1561 aattcttctg agttgtgcgt gcgttcaaat cgttttccaa atggatcgat tccatcttca
     1621 cgcaattgtt ccattttttg acgacgtaca agcatttgat catttaaatc ttctacttga
     1681 tgttgttgtt cctgtgtcac aaaagttcct ccattctcat gttaatctct ttcctataat
     1741 gccaatgaaa cacaaaaaaa gcaagccgaa cttggaattt cggcttgaaa attcacgaaa
     1801 aacttagaaa tcacaacgtt tgtttcaata gtactttttc cgctttttct acaaattgat
     1861 ccagcaggtc attgatttct tgctgttttt ctgtctgatt gattgctact ttcactttcg
     1921 ctgctcgagg aatccctttt aaataataag ccgcatgtgt tctgaattct ctacaagcta
     1981 ctttttctcc tttgagatct gctaagcgtt gtaaatgaag tttagctgta gcaatttttt
     2041 cggctggtgt tggttcagga atcagttccc ctgtttcgag ataatgctgt gtgcggtgaa
     2101 tcatccatgg attgcccagc gctgcacgac caatcatcac accgtctgca ccgacatagt
     2161 caagcatcca ttttgcatct tctggcgttt tgacatcccc gttccccata aatggaatcg
     2221 tcaaatgttt tttcacttct ttcaatactt cccagtccgc ttttccttca tacatctgaa
     2281 cccgtgtacg tccatgcata gcgatggcac ttgctccagc tgattgtgct gctaatgcat
     2341 tttctacagc aaagacgtgc tcttcatccc aaccaatacg catcttgatt gttactggga
     2401 tatttactgc tgaagaaaca gcttggacca tttcgtatac tttctcagga tctaaaagcc
     2461 attttgctcc tgcttctgct ttgattactt tatttactgg acagcccata ttgatatcta
     2521 ttatatcggc agctgtgttt tcttcgacaa attgagcagc ttcaactaga ctatctttat
     2581 ttccgccaaa tatctgtaaa cttaatggat gttctgtttc ttcaatgtgg agcatctcta
     2641 aagttttttt gtttctgaaa tgaatacctt ggtcactgat catttcacat acgactaagc
     2701 ccgcaccaaa ttctttgaca gtgactcgaa atgctgaatt tgttattcca gccatcggtg
     2761 caacaacgac acgattaggg atttccacat tgccaatttt ccatgtgtgt tccacttaaa
     2821 tccctcctaa ggcttctttc ttcaatccat ctaattcttc ttcgctatac ttgtatttgt
     2881 tattacagaa agaacaaacc gcttctgcac cgtgatcttc gtctatcatt gcttggattt
     2941 cttctgcacc taacgcgata attgcttctg caaatttttc ttttgaacag tcacatttga
     3001 attgaacagg cattttttcc aaaatgttca attcttctgt accaagcaat cctttcaata
     3061 tttcttctgg tgtctgtcct tcatctaaga gggtagagat tcttggtgtc gtttttaatt
     3121 gttcttcgat cttgctgatt gtttcttcat cggcacctgg catgatttgg atcatgaatc
     3181 ctcccgccgt cttgatgctg tcgtcagtat tgaccaatac actcagtcca acagctgaag
     3241 ggatttgttc tgagatagca agatagtaag taaaatcttc agcgatttct ccagaaacaa
     3301 tcggcgtttg cccagaaaaa ggttctttta atcctaagtc ttttgtgaca gtaaacattc
     3361 cttgtgttcc aactgctccc cgcacatcga ttttaccttc actatttaga ggaagactga
     3421 gatgcggatt tttgatgtaa cctttcacat ctccctttcc atttgcatct actacaattc
     3481 ctgttgctgg accatcgcct tggattcgaa cagttagttt gtcttctcct ttgagcgtcg
     3541 caccaagtag cagactaccg accaatgtcc gtcccaaagc agctgaggag gtgtgccaag
     3601 tatcatgacg tctttgtgcc tccgctacgg tatttgttgc ttgaacggcg tatgctcgga
     3661 cgaatccgtc gtgagcaagt gccttaacca aataatcttt catttgttat gtactccttt
     3721 ttccgttttt aaactcatag tgaatcatac tagcaactac tgcttttttc aactttggaa
     3781 tgcttctttt aaaaataatt cagaatcatc tagaaaacag gacatcaccc tatttacaac
     3841 ttaaataaag aaatctcccg aaaaccggtt tttgttccgg ttttcgggag tcatatcaga
     3901 tcaattgacc tatatttttt tatttgtatt gatcgtctga atgattatct tctgattgtt
     3961 gagaatctga atcatgttct gctttatttt cctcattcag ttcatgtttt gcttcttcaa
     4021 aatcacgttt ttcttccgct tgtttttcag cgtctttttc ttccaaagca cgttttgctt
     4081 cttcaaaagt tttcgcttct ttttcgcttg gataatcatt tgactcagta ccttcaggca
     4141 tcacaccgtg ttcaaacaat gacttgattg ctttggcatc caatgtttcg aattccaaaa
     4201 gtttttcagc aatcagttta tgctgtgcac gatgagcttc gatgatttct cgagctttat
     4261 catgagcttc catcaagatt tttcgtactt cttgatcaat ctcgaaagcg acttgttccg
     4321 agtaagcttt tgtttgacca tagtcgcggc caacaaacac ttgatggttt ccttcatatt
     4381 gaacaggacc taaacgatca ctcattccat attctgttac catgcttcgt gctagagctg
     4441 tcgcttgctc aaagtcattg gaagcaccag ttgattggac attgaagatg atttcttcag
     4501 ctgtacgtcc accaagtaag cctacgattt gttcaaacat atcttctttc gtcatcaaga
     4561 actgatcttc ttttggtaaa gcgatcatat atccgccggc gcgtccgcga gggatgatcg
     4621 ttactttgtg gacgacccga gcgcgactca atactaatcc gacaatcgta tgaccagctt
     4681 cgtgataagc aaccatttcg cgttcttttt tgctgattac gcgatctttt ttagctggac
     4741 ctgcaatcac gcgatcttcc gcttcatcga tatcagaagc atcgattttc tttttatttc
     4801 ttctagcagc tactaacgct gcttcattca agacattttc taaatctgct ccggcaaaac
     4861 ctggtgtctg ttgagccaca actttcaagt caacgtcatc tgccatcggt ttattccgtg
     4921 catggactcg caagattgct tcacgacctt ttacatctgg acgtccaact aaaatctggc
     4981 ggtcaaaacg gcctggacgt agtaacgctg ggtctagtac atcagaacgg ttggttgcag
     5041 caatgacgat tactccttca tttccgtcga agccatccat ttctacaagc aattggttca
     5101 atgtttgttc gcgttcatca tgtccgccgc ccataccggc accacgttga cgacctaccg
     5161 catcaatttc atcaatgaaa atgatagctg gtgcgttttt cttcgctgtt tcaaacaaat
     5221 cacgaacacg acttgctcca acaccgacaa acatttctac gaaatcagaa cccgagatcg
     5281 agtaaaacgg tactccagct tctccggcaa cagcttttgc taataaggtt ttacctgttc
     5341 ctggaggtcc ttctaaaagt acacctgccg ggatacgggc acctaattca acaaatcggc
     5401 gaggatcttt cagaaattca acaacttcaa ctagttcttg tttttcttct tccgcacccg
     5461 ctacatcaga aaaacgtacg cgattagctt ttttgtctgc ttctttggct tttgatttac
     5521 caaagttcat gacacggccg ccaccgccac cgccgccgcc ttgttgtccc atcatcatgt
     5581 aaaagaaaaa gatgatgatt acgattggca agaagctaag caatagggaa acccatatac
     5641 cgctgcttga ttcttctttt actgttgtag caactttgtt ttcttttgcc agttctgtta
     5701 cttggctcaa tgttgtatca tttggcaaaa tgattgttgt gaaatgggta gtagtcgtat
     5761 ctgttgaacc taagatagct aacccaccgc tgttagcgac ggtctgctgt tctttgtatt
     5821 caccagtgat cttatataca ccatttgcag gctgcacttc aaattctttg atcttgcctg
     5881 cttgtagttg ttctgtgaat tttgagtact cgatatctga cgatcgctgg ccgttattac
     5941 caaaaatgaa ataaacgacc attaccatcg ctaagattac gaggacataa tacaaggcat
     6001 ttttcatcat gccgttgttc tttttgttca tagttgccct ccctaagcgt caatttaatt
     6061 taacagtttt ttatcaatcc tgaccattct attgtaccat aatttaattg agatacagca
     6121 ttaatttgat tgataaacac taggtttcaa tacaccgatg tacggaaggt tacgatatgc
     6181 ttctgcataa tccaagccat aaccgaccac aaattcattt ggtacgttga agccaacata
     6241 atctgcttcg atatttacta ctcttccttc aggtttgtca agtagtgtaa caattttgac
     6301 tgattttgct ttgcggtatt tgaaaagatc cactaaataa gcaagtgttc ttccgctgtc
     6361 aatgatatct tcaacgatca acagatcgcg accttctaca ttcgtatcta agtctttgac
     6421 gatttttact tcccctgacg agactgttgc attcccgtag cttgaaacat ccatgaaatc
     6481 caattccaaa tacgtatcga tagaacgaac gatatctgcc atgaatggta ccgctccttt
     6541 taagacacct actactaggg gattggtgtt ttggtaaatt tctgttaact ctttgcctag
     6601 ttctttgcaa cgaacttgta tctcttcttg tgagatcaaa attctctcaa tatccttttc
     6661 caacatcagg ttctccttcc aattgcttct ttttgatatt tgtaaagaag tatgtagtgt
     6721 attttatcag tttcttcacg aatactcaaa tatgaaaagg caaaaggtat caagctatat
     6781 atatttcttt tggcatctgt aatgatccaa gcatgctgcc tttgtgaatt aggtactttc
     6841 tgatcgataa aatatctaga tacttttttt gtgagattct ctgttaattt aatgcggtct
     6901 cctgcttttc ttttttcgac atacagttgc acatcagcag gaatccaaat gtcatgagaa
     6961 aatagagacc atgaatctgt tgctttgatg tcccctgcat tttcaggttt gaccagacca
     7021 atccattctg tttcattcaa atatattcct tgttctggaa tcagcaacaa gcgctggtct
     7081 tctgtctggc tagttgcttg tttttctttt ttagagagta caacaaaatg atactctctt
     7141 ttcagtatcc aattattgcc aatactgagt tgccattggc tttttttctg ttgccactgg
     7201 tctaaaatcg actccatttg ttgttctttc aaaataattc ctgtttttga gaatgctttg
     7261 tcgaaaaagg agtaaaaaaa atagtatcgt tcagcatctg tcatttcttc aatcatttct
     7321 gcagaaaatc gaaattgttc ctcccactgt tcgacttcct tttcgatcaa ctgtcccata
     7381 tatttttgaa tgacttggtc tgcccattgg atctgttgcg aaaattgctg gaaatgtctc
     7441 aacgcctgtg tattttcttg tttcaattga ggaactacca aatgacgaag ccgatttctc
     7501 tggatatcta acaattggtt tgtctgatcc tcaaagtaaa caagctgcga ttccgctgca
     7561 taagtatata ggtcttcttt gctaaaactc agcaacggac gaatcagtct gcccgtagca
     7621 aatggttgta cttctttgat tccagaaaaa gtatttaact gtcccccacg aacgattttc
     7681 attaacaccg tctcgatttg atcatctccg tgatgagcag tcatcaacgt gtcatattgt
     7741 ccttcttcca tcacgcgagt gaattcttga taacgaaagt tacgagcttc tgtttctatg
     7801 gcggatttgg ctggatcttc ccaaatactt acatataaag ggagattctt ctcttcacaa
     7861 tatgagcgaa gataggcagc ttcttctgct gattcttctc ttaattggtg attgatgtgg
     7921 acgataccta aagaaaaacc gatttggcgt tgcacttgta aaagtaaatg gagcaaaacc
     7981 atcgaatcaa caccacctga taccgctgct aaaattctcg atccttgttg ccagaatcct
     8041 tgttcattcc cgtgccgaat aaatgactgc tgtaatagcg gtgctttttt cccgcctttt
     8101 tcaacgttca tcctttccac accctttctt cctaaaaaaa agagagcggg aaaacgtcta
     8161 aaacgacaat taacctgccc tctgttgata ctatgaccca aatagtcgca ctatcattat
     8221 ttgcggcttt ttagctgcga cgtccgccac ggccaccgcg ctttccttct gtgtttcgct
     8281 tcaatgaaga taagcggtca tcgctatctt tcaaaaatga actcatcaat gaatcgaagt
     8341 cttctttttg agctgaaggt tgactgcgag aaaaagattt tttctgtgct ggacgatttc
     8401 ggttgtcacg gggatctttt tgttgatatt ttttaggacg gctttcttct ccagattcag
     8461 caggttgttc ctgcgctttc cggatagata gtccgatttt tccatcatca tttactgaaa
     8521 taactttaac tgttacttca tctcctacac ttaaaacatc atggatgtct tttacatagc
     8581 catttgacac ttcgctaata tgaactagac ccgtttttcc agcacctaga tcaataaacg
     8641 caccaaaatt tgtgattcct gatacttttc cttgcagttt tgctcctacc tcgattgaca
     8701 taaaaaaaat gttcctccta attatttccc ttttttatat tcctgcttat tttgcagttg
     8761 tagaactagt tgattgatct gtttcatttg tcgagctgtc tgtatttgat tctaatatag
     8821 gataaacttt ttcaccgtct tttgagtaaa agaatttgct tctggcaagt ttagctacat
     8881 actcatcatc tttcagcaat gaaacatctt ggtgcagctg atcaacgtct gcgttgactt
     8941 ctttcatttc agtatttgct tcgactctca attcgttcaa ttgatgcatc ctttgcatgt
     9001 catggaaaat ctgcacacct attatcgcga aaatgaccaa agcaccaaca aaaattgcag
     9061 ccagtctgcg tcttcgaaaa atcagttgac gttgttgttg ctgaaattca gcatactttt
     9121 tctttgcata ttccgtatct agcgcagcga ttttattgct ttttttcgtc acttggattc
     9181 gctccaatct ttttgattgt ctttttcatt ataccaatat cgtgaaagct tgtctaatct
     9241 atttctctag tgtttctgtg cgattttcag aaataatttc gtacattttt tgtgcatctt
     9301 cttttttggt tgattcatgt aattccgtaa ttttcacttc caacgttttg ttgccaaact
     9361 ggactttcaa aatatcgcct actttgacat cactggaaga tttcgccaat ttaccgttga
     9421 tttgaattct tcctttatca gctacttctt ttgctactga tcgtcttttg atgattcggg
     9481 aaatttttaa aaatttatct aatcgcatat cttattcctc actttttaat ttttttattt
     9541 aatcgcagga ttttcttacc aaaaggcaac attagccatt ctcgtactgt aaacagacga
     9601 agacgaatca ctgcataaat gaacacagaa gccccgatcc caactcctat cacgctgaca
     9661 aaaaaagcag tggaacgatg agttacagga ccaaatacta tattcaatac accataatac
     9721 agcagcagta ctactgtcat acaaagaagg ctgaacatca acttccgtcc aaaatgccgt
     9781 tctttccaaa aagcatttag ctcttttgtt tcaattcgaa caaaatacca aagcgtcaca
     9841 cctaatccta aaagggtaga caggcttgca cctgctgttc ctaataaacc ggtcagaaaa
     9901 gaagtagtca atccttttac caatagtccc catcctgcgc cttttaaaga agggcgaaac
     9961 gagttcttgc tttgagcaat actttgataa gcttggatga ctgcagtgaa agcaatggaa
    10021 aaaacaaata atacaagcgc tgcatttccc gcataatctt taaacaacgc ataattgata
    10081 taagggagca gcaaggctaa tcccaaagac gcagctaatg ctaaagcggt cgtcaatcta
    10141 agatagattt tggctgtctg taaaaactga cgatagatcc ggttggtcaa atgacgagtc
    10201 aacgcaggca ggaatgtcgc acttaatgca gtggcaatca ctaaacctaa ttgtactaaa
    10261 ggctgacctc gatcatagac cccttttgct atcttcgcac cgtattctga cagtccgtaa
    10321 acttccaaag cattttttac aaagaaagag tcgatcagct ggaaaaagat aagcaatcca
    10381 ctataaatcg aaaccagtcc gccttctacc agaaaacgcc ggatcaaggg ctttgacggc
    10441 tgtttgaaca atggaaaccg tcggaagctt aacgctcctc catgaatctt ttgttcatag
    10501 tagtgaagga tcccatatgc gcataaaccg ccaaaaacag ctccactcat tgctgccgtc
    10561 cctgtttggt acaccgtcca tccaagcttt ctaaaagcaa acgcactaca gacaatcaca
    10621 aaaacacgaa cgaactgctc aactacttga gaaacagcag ttggttccat caagaatctt
    10681 ccttggaaat tccctcgata aaaagataga ccaggcatca gcaaaaaggt aaaggaaact
    10741 acttggatca gcggtttcaa ttgtatgtct cccatcatcc aagcaatcca gctgctaaat
    10801 gcaaagacaa acaaccacaa aaagacacct gtccaaaata ctaagggata aagattttgc
    10861 aaagcttgct ttcgctcgaa aggattcttt ctttctgcaa cgtactttga tatgaactgc
    10921 ggaagaccgg ataaagctaa agtcattgct atcccataaa ttggataaac ttgctgataa
    10981 acataaaaac cttcgtcgcc gactaagttt tggaggggaa ttcggtaaag ggcacttaat
    11041 acttttgcta tgaaagaagc aatcgtcaat acaaatgctc cttgcatgac gcgccgcatt
    11101 tctttatgat ccaaatcatt gctccttcct acgctgacag atatttttgc tcacgcaacg
    11161 ctttgacaaa cttttggatt tcctgaagcc atactgcttc ttccatttgg ttggggatcg
    11221 tcaagcgtat caccatctgt tctttttcaa cgcctagact ggcttttagt tttgtcgctg
    11281 tcagtgcttc gaataactgt tccacactat agcgttttgt tccaacttta cttagagtaa
    11341 agactatatg cgcctcctgt ttccttatcg tttctaggag cgctcgatcg ccatccattt
    11401 tgatctgccc aattgtcaag agatgtgcta cttcttctgg atattctcca aaacggtcga
    11461 gcagatcatc ttctagttca tctagcattt cttgcgaatc aagttcacga attcgtttat
    11521 agatctcgat tttctgccgt tgatctgcca cgtatgtttc aggtagatac gcatcgacac
    11581 cgaggtcgat ctcaacagtg gtcttctcca cttgactatt ctttccttgt ttacgagaaa
    11641 cagcttcgct tagcatttga gaatacatat cgaaaccgac tgcatcaata aatccatgct
    11701 gctgagcacc taagagatta cctgcaccgc gaatggaaag atcccgcatc gcaatcttga
    11761 atccagatcc cagttctgta aaatctttga ttgcctgaag acgtttttca ctgacttcgt
    11821 taaggatctt ctgctgctca tacataaagt aagcatatgc gacacggctg cttcttccta
    11881 cccgccctcg caattggtat aacgtggata atcccatata atcagcgttc tcaacgaaca
    11941 acgtatttgc gttcgggata tctactcctg tttcaatgat ggttgtagtg acaagaacat
    12001 catattgacc ttcaataaat tcaaaaagcg tattttctaa ttgcgcctct gtcatttgcc
    12061 catgagcata accgatccgt gcttccggaa cgagttcttg gatttcttct actttttgtt
    12121 cgatcgtttc tacccgatta tataaataga atacttgtcc acctcgtccc atttctcgat
    12181 ggattgcttc gcgaatagcc ccaggatttt tttccataac atacgtttgg atcggataac
    12241 gattcgcagg cggtgtttcg attactgaaa gatcccgcac gcctaacatc gacatatgca
    12301 aagttctcgg aatcggtgtt gcagtcagag tcaaaacatc cacttgtgca cgtaactgtt
    12361 tcaatcgttc cttatgtttt acaccaaaac gttgttcttc gtcaatgatc agtagaccca
    12421 aatcgctaaa ttcaatatct tttgacaaaa ttcggtgggt tcctacaaca atatcaactt
    12481 gacctgtacg cagttgttcg atggtttctt tttgttgctt tcttgttctg aaacgactta
    12541 atagcccgat atttacgggg aaaccttcaa atctttccag catcgtttca tagtgctgct
    12601 gtgccaagat cgttgttgga actaaaaagg ctacttgttt gctttccttt atcgctttaa
    12661 aggccgctcg aagcgctact tctgttttcc cataaccaac atcacctacg agtagtcgat
    12721 ccattggttt ttctttttcc atatcccgtt tgatctccgc tgcacttctc aactggtcat
    12781 cagtttcaga gtaggggaag gcattttcga attctttttg gtaggcgtca tcaggtccaa
    12841 aagcatatcc tttttctgac tctcttgcgg catataattt gattagatcg tcagcgatat
    12901 cctcgatttt tgaagagact tttcgcttgg ttttcgtcca ttcacttccg cctagcttat
    12961 tgatgcgagg cgctttggct tcagaggcga catatttttg gatcagattc agttgagtaa
    13021 caggaataaa cagtttgtcg tcgttttgat acaaaatagt catatagtct tggtgaacgc
    13081 catctacttc tagcgtttcc atcccaatgt atttcccgat accatgattg gcatggacaa
    13141 cgtaatctcc tgcttttaac tcattatagc ttttcaatcg ctctgcgttt gttactgttt
    13201 gacgtctcgt ttgttttttc gtcgtttttt ggaaaatttc tttttctgtg atagctacta
    13261 ttttttcttg aggaagttca aaaccagcct gcaaagctcc ttcaactaat tgtacctgtc
    13321 ctctgatcaa tgcatttttt tctgtttcta ctactaatat gtcttcatct cgaagcattt
    13381 gttctgcttt gcgtattctg tcctttgtcg gaataaagat cacaacagtc tgtttttgct
    13441 tgcgccaacg gtccatttct gttttcagca atggcatttg tccgaagaac tgttgcatcg
    13501 tccgatattg aaaattatgg attgcttgga aacgaagatt tcccatccct ttttggaaga
    13561 gtgagaaaaa ggttgtggtg aacttttcct tctgcacgat tttttgtata tctgctgaaa
    13621 atgtctgctc agaaaacacc cgcaattcgc tgattttttg agtctgccat tctgccgctt
    13681 ccctttcgat ttcccgattt gtttccatga ttcgctgata atcatcaacg actagtaaac
    13741 tatttgaagg gaaataatcc aagatcgtca cagattcttg gtaaagaaga tctgagtaga
    13801 attttgcttc ttctgtcggg acacctgctt gccagcttga agtcagctga ccgaagtaat
    13861 cttgcaaaaa ctcgcggtct gcttcttctg tggtcgcagc aagccgtttt tctagcattt
    13921 tttgcatttg ctctattcca tgttctagat cctctggtcc gtagacttgt tcactcgttg
    13981 gggaaaccca gatttcttcc gttttttcga ttgagcgttg tgtttctgct tcaaaatacc
    14041 gcatagaatc tatctctaca tcaaatagtt ctacacgaac aggataggtt gtcgttaacg
    14101 ggtaaatatc gataatgctt cctcgaatac tgaattctcc cggttttgcg accatctctt
    14161 ctcgtacata gcccatcaaa gtcaattggc gaggaagatt ttccacgtct atctcatcac
    14221 caatatgcca attcaattgc gcctgtagcc aagttttctt attcggcagt cgtttgtgca
    14281 aagatgcgac tggcaacaaa tagatacctg cttttttttc ttgaagtgcg ttaagcgttg
    14341 caactctctc gcctcgcgct tctggagaag aaaaggccat ttcagcagaa agcacttcat
    14401 ccactggaaa taaatggatc tgatcttgcg gaatcacatg ttgcagatct tctgctaact
    14461 gattcgcata atacaaattt ggcatcagaa taatcatgtt tttatttgtt ttcttaaacg
    14521 ctcgagcaaa tagaagtgtt ttcgctgagc ccgctaatcc tgtgattagt tgtctaggca
    14581 ttttttcttc taaattgctg acccattctt ttacttgcgg atcttttccg actaattgaa
    14641 taatatccat tccttttcct ccttcctcaa ttgaatcgat tcatcgcatc cacgaacgaa
    14701 tgcccttcta tgagatattc gactgcttta acagattgac acatgctttc ttctatcaaa
    14761 ggctgatttt ctttgctgaa aggactaagc acatgctgga ctactgtttt tttcccttct
    14821 ggcctgccga ttcccacctt gattcgatca aagacattcg tattcaaatg ggaaataata
    14881 cttttgatac cgttatgccc gccggcactt cccttttgac ggagccggat ttttcccact
    14941 gccagatcca aatcatcgta gattacgact aattcctctg gataaatccc aaaataagtc
    15001 attagtgggc cgactgcacg accagattca ttcatgaatg tttgtggttt gactaaaagg
    15061 atcttttctc cattatgaaa aaattcggca acttctgctt cgaacggatt ttttttgaaa
    15121 gtagcttgat gctcgcgtgc aattttatcc acgaccataa agccgatatt gtgttttgta
    15181 tattggtatt tggtacctgg gtttcctaac ccgacgatca ttttcatttt tgttttccac
    15241 ctaattctaa aaagcagcac aaaaggctgg actgctaatt agcggtccag tccatcgagc
    15301 ttttttcttt tttacacttg tcaatgcata gtataacaca aaacaaggcg tttcggctaa
    15361 aagagaatcg ttaataaatg ataaagaaat taaaaatttc ttcaaagttt aatgaaataa
    15421 ttgatatata aaaatagtga aataacgcac atgtaagctt ttacatataa tacaaccttg
    15481 ttatctgtta tattttatag ccaaatagcg tgacatgggg cttgagaaat gttagcataa
    15541 gcatgggaaa aaacaaatca atggaaggat tgatactaaa tgactgcagc tgcagaaaaa
    15601 aaagatcatc aaaaagttat tcttgttgga gacggcgccg taggttctag ctatgccttt
    15661 gctctagtta ctcaaaatat cgctcaagaa gtaggtattg tggatatcaa tactgctaaa
    15721 actgaaggag acgcaattga cttgtctcac gctttggcat ttacttcacc taaaaaaatc
    15781 tattctgcta cttacgcaga cgcacatgat gctgacttag ttgttattac agctggcgca
    15841 ccacaaaaac caggtgaaac tcgtttagac ttagtaaaca aaaacctaaa aatcaaccgc
    15901 gatgttgtca cacaaatcgt tgaatcagga tttaacggta tctttttagt tgccgctaac
    15961 ccagtagata ttttgactta ctcaacttgg aaattctcag gtttccctaa agaacgcgta
    16021 atcggttcag gtacctctct tgactcagct cgtttccgtc aagcacttgc ggaattagtt
    16081 gacgttgatg cacgtaacgt ccatgcttac atcttaggtg aacacggaga ttcagaattc
    16141 ccagtttggt cacatgcaaa tgttgccggc ttgcaaatct atgaatgggt aaaaaacaac
    16201 ccagaaatcg atgaagaagc tatggttaac ctattctttg gcgtacgtga cgctgcttac
    16261 acaattatcg agaaaaaagg cgctactttc tatggtatcg cagtagcatt agcacgtatc
    16321 actcgtgcca tcttagatga tgaaaatgct gttcttcctt tatcagtatt catgaatggc
    16381 gaatatggat tgaacgacat ctacattggt gcacctgctg ttatcaaccg tcaaggtatc
    16441 caaaaagtaa tcgaaattcc tttaagtgac agcgagcaag accgtatggc tgcttctgct
    16501 aagcaattaa aagaaattct agatgaagct tttgctaaac tagacgctga ataatctttg
    16561 aacatgacgg aaaacatcta ttcggtgttt tccgtttttt tattttatca cccttatggt
    16621 ttctaaattt tttcataaag tagagataac cttcgtcttt tcctctaaat tatgctatca
    16681 tgagaccgtt ccaccctaaa cttttactta aggagttgtc gatatgacaa gatcttcgca
    16741 tcgaaaaaaa tcgaaacatg ttgctttgct ttcagttctt ggtgttctct tagtgctgat
    16801 cagcttttat acctatcgca gtacatacta cacaaagcat tttttgccga atacagaaat
    16861 aaatggaata aatgtcagca atctaacagt cgaaaaagca aatgaaaaat taaaagaagc
    16921 ttattctgat aaaaaactgg ctattaaaga agatggaaaa gtctggcagg aagtaccaaa
    16981 atccgagctc ggctacaaag atgatttcac ttcggagctt tctccgatcc taaatgaaca
    17041 aaacggatgg acttggggaa tgacttatgt ctcagcggca gaaaaacaag aaatagatcc
    17101 tgtcagtcaa aaccaccaaa aactagacac taccgttcag acattgacaa ctaagctgac
    17161 tgatttgaac aaagaccgga cacctactac tgatgcaacg atcgaaaaag caggtgactc
    17221 tttcgttatc aagcctgaag tcaatggaaa tacgatcgac gtcgatgctg cggtcaaaca
    17281 attaaaaagt gcggtaaact ctggtaaaga tacaattgaa ttgactgaat tcaaagaaaa
    17341 accaaaagta acatctgaag acagctcact gaaagaacaa ctcgcatcca tgaatgctat
    17401 cgcaaatgta aaagctactt acagtataaa cggtgagact ttccagatcc catcaagtga
    17461 tattatgagt tggctgacct acaatgatgg aaaagtcgat ttagatacag aacaagtacg
    17521 tcaatatgta acagatttag gcacaaagta taacacaagt acgaatgaca caaaatttaa
    17581 aagtacaaaa agaggagaag ttacggtccc agttggcact tacagttgga cgatccaaac
    17641 agacagtgag acagaagcat tgaaaaaagc gattctggca ggccaagatt ttactcgttc
    17701 acccattgtg caaggtggca caaccgctga tcatccattg atcgaagata cgtatatcga
    17761 agttgatctg gagaaccagc atatgtggta ttacaaagac ggaaaagtgg cccttgaaac
    17821 agatatcgtt tccggaaagc caactacacc tacacctgct ggagtcttct atgtttggaa
    17881 taaagaagaa gatgcaactt taaaaggaac aaatgacgat ggaacacctt acgagagtcc
    17941 ggtaaactac tggatgccga ttgactggac aggtgttggt atccacgatt cagactggca
    18001 acctgaatac ggtggagact tatggaaaac ccgcggttca cacggctgca tcaacacccc
    18061 accaagtgta atgaaggaat tattcgggat ggtcgaaaaa ggaacgcctg tattggtctt
    18121 ctaacaaaaa taatatataa aaagaaagca cccgtacgat tggtacaggt gctttctttt
    18181 tattatctaa caatgtgcta cttacatttc agcagcagta aagacttctg aaacatcatc
    18241 atcgtcttct agcttatcta ctagacgttc caattgctct ttttgttcct catttaaatc
    18301 aatcgtcgtt tgtggaatca tcgtcaattc tgcttgcgcc aaagtgaact ctttttccaa
    18361 ctcatctcgt acagcagcaa aatcttctgg tgcagtataa atctcgaata cttcaggaga
    18421 agtttgcaga tcttctgccc ccgcctctaa tacatcctca aacatcgtct cttcatcaac
    18481 agtcaaccca tcgcgttcaa tagcgatata tcctttgcgg tcaaacaagt aactgactga
    18541 acccgtttct cccagtgatc caccgtttcg agtaaaagct actcggacat ttgtcgctgt
    18601 ccgattacgg ttatctgtca atgcatggac taagattcct actccacccg gaccatatcc
    18661 ttcatacgtg atttcatcgt aattcgtttc atctacggca ctcgtagcct ttttaatcgc
    18721 ccgttccaca ttgtcgttag gcatatttgc agcttttgct ttgtccacaa caagccgcaa
    18781 agctggattc atagatggat caggaccgcc tgctttagct gccatataaa tttcacgaga
    18841 aagtttctga aagactttcc ctcgttttgc atcttgggca ttttttcttc cttggatatt
    18901 atgccattta ctatgtcctg acatgatttt tcctcccttg ttcttttgag aatacacttc
    18961 tattctagta ctatttcaca aaaaaacaaa gccacagcca atctataaac gcttcttgaa
    19021 tgccgcaaaa agcacacata atatcacagc agtcagagca ccgatcgaat ccaacatgac
    19081 atcttggaac ataggtgttc gtccaccggt catcatctga tggaattcgt ccaaacctgc
    19141 gtaccctgtt gctgaaagcc aagcaaggat cgctgtcagc caccaaattt ttagcttggg
    19201 gaaaacacct aaaaacaagc tgcctcccaa aataaaatag gtaccgaaat gtgcgccctt
    19261 ccttaaaaag aactctaaaa atttggcgta gcccagatgg ttgatactca ccatacttcc
    19321 accataatta aaacgtatac ccgctaatgt gtctttaaat ggctgattag gcagccagct
    19381 ttctaatcgc gaaacttgtg attgctgtcc gtaagtttga gaagaactgt aaaataagat
    19441 tataacgact atcagtgcga ttactaaata aatatttccg tttttcattt gtttttttga
    19501 agaattcata agtcacctct gtattttatc ttagactttt tttgaagaaa caacaatcat
    19561 tttcatagag acttcttcca taaaaaagtg ctatacttaa ttttgtatta caatatgaag
    19621 ggagtttttt catgagttat tcattaaatt ggaatctaga ttctattttt tccggaggca
    19681 gtcattctga tgctttgaac caacggatga agcaattgga agaccaaatg aacgagtatt
    19741 atcaccgcgt aacaaaatgg tcacctagtt ctgacaatgc agagcagtta aacgctattc
    19801 ttcaattgca agaaacgatt acgaatggtt ttacccaatg cagcagctat attactgcat
    19861 tgctttctgc caatgtcaat gattcagatg caaaggtctt atcaggcaag ctttacgcaa
    19921 tgctgcctcg tctgcaatca gccgaaactg tattgtcaaa gaaatttgca gaaatatctg
    19981 acaatgattg gaatcagatg ctttctcaat cttcgtttga aacaattgct tttcgtttaa
    20041 acgagattcg tcgtgatggc tcacaattat tatcagaagc tgaagaaaac attataaata
    20101 cgctttcttt agatggtcta aatgcttgga gcagtcatta tgatacgatt gtagccagca
    20161 tttcgattcc cttcgaacaa gatggacaag tagtcgaatt atctgctgga caagccttca
    20221 ataagatgat gggcgatcca gatccaaaag tcagagaaac acttttcgca gcatgggaag
    20281 aagcctggaa agaaaaggca cccctcttca cggatacttt gaatcattta gatggtttta
    20341 gactttctga ctatgaactt catggtgtca cagattttct acaaaaacca ttagagtaca
    20401 atcggttgaa gaaagaaaca ttgtctgtca tgtgggatac aatccagaaa aacaaacaac
    20461 cccttgttaa ttacttgact agaaaagcta atcttttcgg taaagaaaaa atggaatggc
    20521 aagatcaaga tgcacctatt attttaggcg atttgaaaga aaaaaccttt agttttgacg
    20581 aagcagcagc atttattatc gaaaacttta acaaattcag tccaaaaatg gctaaatttg
    20641 cccaatctgc ttttgaaaag agctggatcg aagcagaaga tcgtcctggg aaacgtcctg
    20701 gtggttattg tactgagtta ccagaaacaa aagaatcaag gatctttatg acctacagta
    20761 attctgttaa tgaggttgcg acacttgctc atgaactagg acatgctttc catagcagca
    20821 cgatgtggga tttaccttct ttaaatcgcg aatatgccat gaatgtcgca gaaacagcaa
    20881 gtacattcgc agaactgatc gtagccgatg ctacattaaa agtcgcaaaa acaaaagaag
    20941 aaaaaatcaa tttgctagat acaaaactgc aaaatgcttt agctatgttc atgaatatcc
    21001 attcccgatt catttttgaa aatcgattct atgaagcacg gcaagaagga ttagtctcag
    21061 aagataagat cacagagatg atggttgagg cgcaaaaaga aggctatcaa ggagctttag
    21121 ctacttacca tccatatttc tgggctgcaa aattgcattt ctttattgat gacgtaccgt
    21181 tttataactt cccatacaca tttggctacc tctttagttt gggcatctat gcttacgcca
    21241 accaaaaggg agcaaatttt gaagaagaat atatccattt actacgcgat actgcctcaa
    21301 tgacgacgga agatcttgcg aagaaacatt tgaatgtcga tcttacccaa ccagattttt
    21361 ggcaagcagg tatcgacatg gtgactgaag atatccaaac gttcatggaa ttgacagaag
    21421 aatttatctg atttctattt tatcacaata aaaaaagagt tagtccagaa tgggctaact
    21481 ctttttgatt aatcattctt acatgctgac agcaaagtca ggggcaaacc attgaactga
    21541 accaactttt acgctttcgc ctggttgagg agcatggata tattgtcctc cgcctaaagc
    21601 aatcgctacg tggtaagttc cgccttgtga cccccagaat aacaagtctc cagcttttgc
    21661 ttgagaaact gaaatttttg ttccagctga ttcttgagga actgtccaac caccgatgtc
    21721 acgaccagtt acttgcatgt aaacatagcg tgtgaaccct gagcagtcaa agccacttgg
    21781 atctttaccg ccccaaacat atggagtacc aatatatttg taagcttctg ctacgattgc
    21841 tgcaccattc acacttccag aaggagctgg agcaggtgtt acaggttttg gttgttcagg
    21901 tgttggtgtt ggcgttggag ccggagtact acttcctgtg ccgtttcctg tatcaacact
    21961 ttgatctgtt gatggtgttg taggcgcagg tgttgttgat tcttctgttg ttgaagattc
    22021 aggcactgtt gttgattctt ctgttgctga agattcaggc actgttgttg attcttctgt
    22081 tgctgaagat tcaggcgctg ttgttgattc ttctgttgct gaagattcag gcgctgttga
    22141 ttcttctgtt gaagattcag gtgttgttga ttcttctgtt gctgaagatt gtgtagctga
    22201 tgatgattct tcagtcattg aagattgtgt agctgatgat tcttctgttg ctgagctttg
    22261 tgcagtagat gaagcttgag tagcttgtgc ttcagcctct gcttgttcgc gtgcttcttt
    22321 ttcagctttt tcttgtgcag cttgttgacg agcttgttct gctagacgtt gttgttcacg
    22381 aatacgagct tgctctgctt cagcttctgc tttttgacgg ttcaagtcag ctttcttgtc
    22441 ttcagcagtt gcttgttcag cagctaatga agttttcagt acgttcaaat cagcttgttt
    22501 agataaaaga tcgccttttt gagattccaa agcagcttgg ttttcagcta attcttttag
    22561 tttagcatcg ttttcagctt ttttgtcttc tacagctttt ttatcttgtt tttgttgttc
    22621 catcaagtcg ttgtttgctt ttaccatcgt tgtcattgct tgaacgcgtc caatagcatc
    22681 tgctaatgaa tcagcattca atacagcatc gatgtagttt gagcttgtgt tgcttacttg
    22741 tgcttcgcgt gcttgttttt ggattgtatc ttcacgtttt tcaatacgtt cttgtaaatc
    22801 tgcaatgtct tttactaatt gtgcagattc ttgacgcaat gtatcttgtt ttgctaatag
    22861 gtcttgtgct tgtgtgttga tttcagaaac ttggctttct aaggcatcga tttgtgattg
    22921 tgcatctgct tgttgatttt tcaagtctgc aatcttttga tcttgctgtt gtatctgaga
    22981 atcaaaatcg tccgccgcgg cagcgatcgg tgatgctacg gcagtcaagg tcattgaaca
    23041 taccattact gctgatatta aactcttttt cactcttcat tcctccgact ggcttaattt
    23101 aataaatatc taaatatatt taagtctttc ttaacgatat aaaaattgta ccatagcagt
    23161 gtgtcaacga tataatagtt atgttacaaa aatgtttcaa aacaactttt aaaaatgaca
    23221 aaactgttcg tttcagaaaa tgatcatgaa aaagccgcgt taagtctagc atttcttaag
    23281 ataacaacga ttctttttcg ctttcgacct aaaaataaaa aaagaaatta ttcatcactg
    23341 aataatttct taaagggaaa aatgaataat acaaatataa tcatgttcaa cagcagtgta
    23401 ggtcctaaaa accttgtaat aaaatacgtg ttatttacta ctgctaattt aaaaatcaac
    23461 tggaccacca tcgtaaacaa ttcataaccc gtgacaaaga tgatcatact gaaaaattca
    23521 gtaaagatgt tgacatggat aacgtctttc attacataca tcaaccatac aattaaaggg
    23581 agagctactg catagatacc gataacgcca atatagtaag cgtcatagat agccccgaga
    23641 acaatcgtgg taatcagtaa ataacgtttt tcaaacgcca tactgcaaca taataatgcc
    23701 aatatcagaa agtgggcatt tgacatatag gtgcctttcg accactcccc tagcattctt
    23761 gttaaatgac catctatcaa catcaaaaag aacaaaacga ttggcagata gtacttcatt
    23821 gtttctttct tcaacatgga ctaatcctcc gctagccgtt ggacaatcgt cacaactgac
    23881 acgtcataca tttctgcata aggtttcaca taaacttccc gatccagccc ataactatct
    23941 ggtttcgttt taataaccgt accgatcggc aagttagctg gagaattccc tccaagaccg
    24001 gatgtctgga caacatcccc ttctttgata tccgtatcac cagttaattg ggtcaccact
    24061 aaggcatgga gcttttcatc atagttttta agcaaaccaa acgcttcgcc attagccgaa
    24121 gatacccgta ctggaaaatg attggagctt tcattagagg atgacagtaa ttcgatttta
    24181 gacgaagccg tattgacctc gatcactcgg ccaatcaatc ctttttgcga catgactgcc
    24241 atatttgctt cgataccgtc tttagaaccc ttgtctacga tcagcatgtc ctgccaactg
    24301 tcaggcgaac gagtaatgac atttgccgtt actttttcgt aactagtcag cgtctgattc
    24361 aactcaagtt cttttttcaa cgcttcgatt tctctctcgt aattcttgtt ttgctgtgaa
    24421 acgtcatcat agctatcgat tttttctttt agccgctcat tttcggcata agttgaaaaa
    24481 agattatgga cagaatctac tccatttcgt acccaccgta ctggggcaga gattgtttta
    24541 tcaaccattg caacactgtc attaacgatg gattggaaga aattgggctt tccttcattc
    24601 gctcgctgcg cagcggtcac acttagtatc gtcaccacga tgataacaat aaccaaggta
    24661 ataataatat ttttattagg attgaacttc ttcacaaaga cacctcgaat taattagact
    24721 cttgtgttca attttatcac taaatgccgt gaaacaaaag gtaagttgct cctcttaagc
    24781 ttttttttag caaataatag attctgtcat ttttagcctg aaagacaaat ttttttattt
    24841 gtttatggat caatctttgc tttgaataac tgcaatcata ccatctgaaa aagaaaattc
    24901 tgcggtatct gaaagaaatt cgttacttgc ataagatgtc ggatattcta cttctttatt
    24961 ttccaataca tacttgctgc cttttaatgt aagattcgag acgggagtca agcagcaata
    25021 agctaaatac ttcatatctg gtatctgtct tactatatgc tctccaggca aataataaga
    25081 caaataattt tgacaatctc tgataataag ctgatgggca aagggtttga agcgaggttc
    25141 catacctaac cagagattgg ctaaaagatg atccaatctt ccgcccgtag ctccaatcaa
    25201 agtgacttcc gcttgcgggt aacgttcaaa tgtttttaag atacctaact gtgtatctgt
    25261 atcgtctttt tctgcagggg aacgggtata ctctttggct tttgcaaata cttccttctg
    25321 ctcttcgttc gacaatgaat caaaatcacc cactgccaga tctactgggt agcctgcttg
    25381 aagcaggtac agcgcaccac ggtctatccc gacataggca tcgtatttgg acagcagaga
    25441 ctgcggccag ttttgcggat tacctccagc agctaataaa attttcattt agtctaatgc
    25501 ctcttttaat cgttcgattt gtccgactgg attttcggct ccatagatat atgatcctgc
    25561 aacgaagaca tccgcccctg catctttaca gatttttgct gtttctggaa cgattccgcc
    25621 atctacttcg attgcataag tccaattgtt ctctctacgt agatcagcga gttccgtaat
    25681 tttttctact gtttcttcaa taaagctttg tccgccgaac ccaggattga cagtcatgac
    25741 tagtacttga tcagccaaag gcaaaacatg tttgatcatg gagactggtg ttcctggatt
    25801 gataactact ccagctttaa ctcctaagct ttttgtcatt tgaagtgccc gatgaatatg
    25861 aggagttgct tcgacgtgga cagtaatgat atctgctcca gcttttgcaa atgctggaat
    25921 gtagttttct ggatcaacga tcatcagatg gacgtctaac ggtaatttag taacaggacg
    25981 gattgcctga acaatatttg gccctaatgt gatatttggg acaaatcggc catccattac
    26041 atctacgtgg atgtaatctg cgcctccttt ttccactaac tcaatgtctc tttgtaaatt
    26101 ggcaaaatct gcactcaaaa tggatggtgc tatcttcatt cttgtttcct cctattttcg
    26161 tttaccttct ttttttgcat acattggttt tcttttttcg atttcttgta agaattgcaa
    26221 ataattgtcg taacgtgtct gtgcgatacc acctgtttct actctatgtt ttacttcgca
    26281 gccaggttct ttagcatgca tgcactctct aaatttgcaa tggacagaag cttctacaaa
    26341 ttcaggaaat tgtctcggca gttctcctgc ttcaattgtc aaaaagtcaa tcgagctaaa
    26401 tcctggtgta tctgctacta acccgccata gataggaatc agttccacat gacgtgttgt
    26461 atgccgtcct cttccaagtg catcagaaat ctctcctgtt gctaaattca gttccggtga
    26521 tatttgattc agtagcgtcg attttcctgc tccagactga cccataaaga ccgatagccg
    26581 ttctgggaag taacggatca gttctgtcgt atcacccgta tgattgggta caatcaccgg
    26641 atagcctatt ttagtatagg tttgcttgat ctcttcgatt ttttctgggt cgtctgacaa
    26701 gtcggtcttt gtcaaaaaaa taatcggttc gatcgactcg tattccaagg taactagaaa
    26761 gcggtcaagt agattataag aaaatgcagg ctctacgata cttgtgacaa tgattccaag
    26821 atcaacattt gtgactggtg ggcgaactaa ttgatttttt ctcggcaaga tttccagaag
    26881 atagccgtct gtttgatttt cactctcaaa gatcacctga tctccgacaa gcggcgtgat
    26941 tttccgattc cggaaatttc ctcttcctct tgtttgatag gtctctccgt ttgcgtatac
    27001 atagtaaaaa ccgctgattg cttttctgat ttgtcctttg ataagtgcca cctccgtttt
    27061 tgtttatttt accatacgaa gaggaaagta ggtgttattc catgcaaact tctcaaagta
    27121 tatggcttga aaattgatca aaagagatag aaacacaaaa aagagaatgc aacaaatcaa
    27181 tgtcgcattc ccttctgata tattttattc agcacttatt ttgaactttc tgaggtgcta
    27241 gctgatgaac ttgtggagct ggaagattct tcttttgaag agttgcttgt gctactggag
    27301 ctacttgagc tgctagaact gtctgaagag tctttttcag aactagaaga cgctggatct
    27361 ggtcctttag agtaaattac gttcacaacc attcctttgg taattgtcgt tcctgcccct
    27421 ggtactgtac gaatcacttt atctttatcc accgtgtttg aatattcgga agtctcatgc
    27481 ccgatatatt ctgcaccgac actagctaaa taactttgtg cttcgctttt tgtatagccg
    27541 gagatatcat tcaatttagg ctctgttcct tggcttacga caaaattgat gtaagtttct
    27601 tctgctacaa cttcacccga ggctggatcc tgactgatga tcgaacctga tggtacagtg
    27661 tcgctatagt cataagtagc actaatatta ttgatattga atccttgtgt aacaagccaa
    27721 ttacgaacag tttcgtagtt ttctcctaca tgactactca aagtcactgt ttctggtcct
    27781 ttgctgactg tgaaagtaat cgtgtctgtt ttaggatcga cttctgtatc ttctgatgga
    27841 gattgcgaaa tgatcattcc gctttctacc tctgaactga attcctcttc tttttgaatc
    27901 aagtcagcag aaaaccctaa ttctttcaaa cttgcttcgg catcttcata acttttacca
    27961 ctgtaatcat ctaatttgat ttttttcgtg ccgctactaa tatacagagt gacttcgcgt
    28021 ttttgcttga ctgtcgttcc cgctgccggg tctgtcttca ctacattacc ttcttcgatt
    28081 ttgtcatctg caatttcttc tgtttttgtt gctacttcta atttcgcttt tgcgagtgtt
    28141 tctctagcag aagcttcaga aagatcagaa acatcaggaa caacaacttc tcctcgcccg
    28201 ccagaagcaa agaatacagc tgttccgatt cctaaaacag caagaatagc taataggatc
    28261 agccaccatt ttttctgtcc tttcttttta ggatcaggca gaacttcttc ctcctctggt
    28321 aaggtttcgt tttctggctt gtcttttggc agaggcatag atttgaatga atcaggcatt
    28381 ggtgcatctt ccgtaagggg tgtgataact tttgtttcat tatccatcac atgaggctgc
    28441 catttttctt cgtttgcacg agctggtgat aatactgtag aaagatcatc agacatttcc
    28501 tcggctgact tataacggtc tgcaggttct ttagccgttg cccgtaaaat cacgttttcc
    28561 agtgcttgtg gcacatttgg atcaagtgct tttatagatg gaagatcatc ttgaaaatgt
    28621 tttaaagcaa ttgttacagc agattcacca tcaaacggta cgctacccgt caacatctca
    28681 taaaggatga tccctaaagc ataaatatct gactgcttcg ttgccatact tcctctggcc
    28741 tgctcagggg acagataatg aacagatcct agcatcgtat tcgtctgcgt gatcgatgtt
    28801 tcagataaag cgatagcaat cccgaaatcc gtgattttta caacaccttc attatcgatc
    28861 agtacgtttt ggggtttcaa atctcggtgg atgatttgat ggttatgagc taaagaaata
    28921 gcagaaagga tctgctgcat aatatttact gctgtttcat aaggaacagg ataatgtgtt
    28981 tggatatatc gtttcaagtc cattcctttg acgtattcca tcaccagata ctgcatgttg
    29041 tcttcttcac caacatcgta aacactaaca atgtttggat gaacgagttc tgttgctgct
    29101 aaagcttctc tctgaaatcg tcgaatcgca gtctggtcat tttgaaagtc aaatcgtaag
    29161 actttaactg ctacatcacg atccaagatt aaatcgtgcg ccaaaaatac atttgccata
    29221 ccaccgctac cgatatttcc agtgatttga taacggccat ttaatttttt gcccaactcg
    29281 atcatacact gtcctcctta tattcgataa gaagaacggt gatattatcc gctccgcctg
    29341 cttcattggc acgatcaatc agctcagtta ctttatgaga caatgggcct tcttgattga
    29401 tgatcgttcc tatttcttct tcagacagca tgttcgtcaa gccatctgaa cataacagca
    29461 gtcgatcttt tagttgccaa agatgagtag aaacatctac ttcgactgtt ccaggcatgc
    29521 cgacagaacg tgttaaaata tttttccttg gatggttcac tgccatttct tcgctgattt
    29581 ctcctgattt gactagttca ttgactaaag aatgatcctc cgtcaattgc atgattttat
    29641 cttcacgaat caaataagct ctgctatccc caacattggc aatcgtgaac cagtcatctg
    29701 acagagcggc cgctacgatt gttgttccca taccgttata ttccggctgt tcttgtccac
    29761 gctgataaat cagcatattt tcttcttgaa tcgattgaat gaaccattgg gcgatttttt
    29821 catcgtcaac agcttcttgt ttttcccaag cttcgcctag attcgttacc gccatctgac
    29881 tggcaatgtc tccggcacga tgaccaccca tcccgtctgc taatacagca agcttgagac
    29941 ctgaacggct ggtaaataca ctggcatagt cttgatttgt gttacgtcgt cttcctacat
    30001 ctgattgata ttcaatctgc attttttcac ctcattactt tttgcgcaaa caacaaatga
    30061 aaaatccatc tgtgtaaaac tgatgcggat acaaagtcaa cattttctct tcgattgatg
    30121 attgtagcat aggattggtc aaaacatcaa tcctctcaaa atcagggtgt ctttccaaga
    30181 aagcagaaac aacgtcttgg ttttcttctt ttaagatcgt acaagtgcta taagtcagaa
    30241 taccggaaga ctttaaagtt ggtgcgcagc tttctaaaat ttccaattgg atttttggta
    30301 aattcgctaa ttcatccggc tgttttttat aacgaatatc tggttttcgc cgaagcagcc
    30361 caagacctga acaaggcgcg tctactaaaa tccgatcaaa ctgctctgct tcgaactgtt
    30421 tactgacttc tctagcatcc atcttttgag cgtagacagc agaagagaca cctagacgtg
    30481 ctgcattatc ttcaatgagt ttgactttgt gttcgtgtac atctaaagcg accacttctc
    30541 caccgcatga cggatctaaa aaagaagcaa tatgcgtcgt cttacctccg ggtgctgcac
    30601 aggcatctaa gacatgatgt tcaggctcga tttgcataga cggcgctaca agcatcgaac
    30661 tttcatcttg gatcgtcatg acgccatcgt ggaacaatga gctagaagct aaatgccctt
    30721 ttttcgctac tataccgtat ggactgactt ggcttttttc agctgtgatc tcttcttctt
    30781 gcaaaacagt gatggcctga tctctggaaa gtcggcgtgt atctactcgg gcactagcat
    30841 ggctaggctg atatagcgat aaaccgagct tttcagtttc ttcgtatccg atttgatcca
    30901 ccagtcgttt cgtcatccat agaggcaggc tgattttgac agaaagtctt tcgataggat
    30961 cagcaatgcc ttgcagatct ggtgctcctt gacgctgata attcctcaag acaccattta
    31021 caaatttccc tgtcccagga tttcctttta cttttgcgat ttcgaccgcc tcgtttaaaa
    31081 tagcatgtgc agggacttta tctaaaaatt ctaattggta tacagaaaga taaagaagca
    31141 tcttgaccca gtcatccact ttttttgctt tagcgatcaa tggtgcaacg taaaattcca
    31201 atagtcgctg atggctaatc gtaccataga tcaattctgt aaataaaccc acatcttttt
    31261 gatttagctc gctttgtttg atcatctcat tcaacaaaag attcgagtag gcgccaccct
    31321 tgttcactcg ctctaatgtc agtaatgcca tatatcgtac tgattttttc atttttttag
    31381 ggattttttt cattatttca cctgctgacc aatctgaact tgctgaccgc ttccgttaag
    31441 gaaatcatgt acggcctgtt ttcctttacc agctggctgt aattcattga tggctaagac
    31501 ggtctgttta ccgcaagcaa tccatagatt ttttttgtct ttccggatga tcgtaccagg
    31561 tgctttgtct gtagtttctg ccaatacttc aaccgataat aatttccagc gtatgccctc
    31621 ataggtcgtg aatgcaattg gccaaggacg cattccgcgt acttggttgt caatttcttc
    31681 tgcagtttta tgccaatcga ttgcttcttg ttcccgtgtg atgtttggcg aaaaagttgc
    31741 ttctgactcg tcttgaggta ctggtttttg tccatccaaa atattgggca atgtttctag
    31801 caatagcttt cttcctaaaa ggctaagctt ttcaaacatc gtccctacat catcttgttt
    31861 agtgattggt atgctttctt gcgcatagat tccgcctgca tccatttttt tgatcatttc
    31921 catgatcgtt acaccagttt ctttctcccc attcatgatg gcataatgaa caggcgcacc
    31981 tccccgatat ttaggaagta aagaagcatg gacattgatt gcccctaatt ttggtacttg
    32041 caataacttt tcaggcaaga attggccaaa tgccgcagtg acgatcacat caggcaccaa
    32101 ttcaatgatt tcttccattt ctggcgaacc ggagattttc tctggctgca gcacacggat
    32161 accgtgcttc agagccgctt tttttaccgg tgtcggcgta attactcgtt tccgcccaac
    32221 tggacggtcg ggctgtgtta ccactgcctg gatctcgtaa cctgattcta tcagcccttc
    32281 aagaatcggc acagaaaaag caggtgtgcc cataaatact agcttagtca tcaagatgtt
    32341 cctccatata ttcttcaagt tcttcttccg ggatctgctg tatcattttc tcaatgaaca
    32401 agataccgtc taagtgatcg atttcatgct ggaaggcacg ggctaaatag ccaaaagccg
    32461 tcacttctat ctcttctccg tctcgatcat aataacgcac ggtcacttca tctgctcgct
    32521 tgaccgttcc atagacatgc ggaatactga gacaaccttc tacgtcaagg ctttcacctt
    32581 ttgcttcgat gatttctggg ttaatcagtt caaatttctc gccttcatct acttcgatca
    32641 cagcaattct cttgttttgt cctacttgag gagcagcgat ccctattccg tcattcgcaa
    32701 tcattgtctc atataaattg tctaataaag cgattgtctc gtccgtgatt acatcgattg
    32761 gctgagcagt tctttttaat ttatcattag ggtggatcaa tataggatag cgcataattt
    32821 ggccctttct taaataaagt ttaatggttc gttgtcaatt gccacaaaaa gaccttgtcg
    32881 ctgttctttc tggcttccat tcaaaatttc atccaaaaca tgaggaagat tcggttcatg
    32941 tttatatttg atgattagtt gataataata tcggtttttc actctcaaaa tcatgctggg
    33001 cgttggccct aatagcaagc tttgagggct taacacttct tttagctgat tggcaatctg
    33061 aaagattttc tttgccgcta cttgttcttc aggatggctg gcggttattt tcacagtaaa
    33121 gtaataagga ggatagcctc cacgatgccg tacatgcatt tcctgctgat aaaactgctc
    33181 ataattttgt tttttagcca attctatggc gtaatgttga ggattgaatg tttggatcac
    33241 gacttcgcct gctttttccg ctcgccctgc tcgtcctgaa acttgggtaa gcaattggaa
    33301 tgtatgctca ctagaacgaa aatccggtaa attcagggag gtatcagcat tcaacacccc
    33361 aactaatgtc acttctggaa agtccaaacc tttagcaatc atttgtgtcc ctaataatat
    33421 atctgcttct tttgcaccga atttttgtaa aatctgttcg tgtgcccctt ttcttcgagt
    33481 ggtatctaca tccatacgca aaatcctagc ttgcggataa agttctcgga gttcctcttc
    33541 tactttttga gtacctgttc catagtagcg gattttattg ccgccacaat taggacagcg
    33601 attgggaata ggttcttcat gtccgcaata atggcagcgc attgaacgtg tatccatatg
    33661 cagtgtaaga gagatatcgc aattaggaca tggaagaaca aatccacagt ccctacacat
    33721 cacaaaagac gaatatcctc gacgattgag cagtaggaca gtttgttctt ttttgtctaa
    33781 tcgattttgt attttttcct gcaaattagc agaaaaagta gaggtccgat gattctgaaa
    33841 ctcttcacgc atatctacga tctcaatagc tggaagctgt gcagctgctt tggctcggtg
    33901 gtttaactgc aataactgat aaacgccttt ttgagctcgt gctcttgatt ctaaagaggg
    33961 cgtcgcgctt cctagaacta ttgggcaatg atgatattta ctgcgccaaa ttgctagatc
    34021 tctcgcatga taacgcggtg tctcatcttg tttgtaactt gattcatgtt cttcatcgat
    34081 aatgatcagc ccgatatttt tcagcggggc aaaaatagca gatcgtgcgc ctactactac
    34141 ttgcgcttct tcccgctcaa tcttgcgcca ttcatcatat ttttcacctt gggaaagtcc
    34201 gctatgtaag accgcaactg attctccgaa gcgtcctttg aatcgctcga ccatttgcgg
    34261 agtcaatgcg atttcaggca ctaacataat agccgtttta ttttctgaca aaacatcagc
    34321 gattgcctga agatatactt ctgttttccc gctgccagtg attccctcca gcaaaaagac
    34381 ctgatctttt tgctgctgac ctgctgtgat gatttgttcg acagcatttt tttgttctgc
    34441 attcagttcc aatgccgtcg tttggtcaaa tactcggtct ttataagggt cgcgataacg
    34501 ttctgattct ataaactcca gccagccctt ttcttttcct tgattcaaga cagcagtact
    34561 gattccttta tttttgaaat aagcaattgg ttcttcttgg ctcagttcag ataaacacaa
    34621 aagaagttct ttttgtttct tggcattttg ccgtaatttt gcccattctt cttcgatttg
    34681 cttttcagtt aacaaagaac ggaccaaacg aatcgttttt actttatttc gtgtatggac
    34741 ttcatatttg atatctacta attgctcttg gcgccagcga atcaattgag aaaggctgcc
    34801 ttcttcttct gcttccttcc aggaaatcac ttctcgctga tcaaacaata cctgaacttc
    34861 tggtgtgtct gccaaaggat agatcaattt atcatattct gctttcatca cactcggaag
    34921 catcgtttgt agacaagtga ttttgaatgc gtaagtaatt tttttcatat aatcagctaa
    34981 tgaaagtaat tcttcattga ccaccggagc aaggtctagc aaacgaataa ctgccttcaa
    35041 agatggatta gctgattctt ctgatttttg gatagccata acaaatcctt ggacatgacg
    35101 attgccatta ccaaaaggga cttctactcg catcccgact tgcaaagctg tttccatttc
    35161 agagggaacc aaataagtga atggctgatc ggtctgcata gtaggtacgt ccacaatgac
    35221 ttcagctgtt aatgtcatgg ttcgtttcct cctttttttg tcttttctta tcaagcgaca
    35281 aaaagttcct tgcctatttt actagagatc gatggatttg tcttatcttc tgccaaatcc
    35341 taacaaaaaa ttaacagcag ataagaaaaa aaagaactgt gtcaaaaagt tcagacacag
    35401 ccttttctac cagacaataa gtggcgaatg tttgaaaagg aaacagctgg gtttgctttt
    35461 ctttttagat gttcgctcta tttttcttta ttacattttt tgctcttcac ggatacgtga
    35521 ttccaattcg tgttgttcac gctctttttg tgcttgacgt tcctcttctt ccatcttcaa
    35581 acgttcacgt tttaattcag gatgtggatc attgactaca tttcctgcat cgatttcttc
    35641 taaagcttga ccgacgcttt ttactgaatc aaaagaatct agagtaggct gtgcacttgc
    35701 atccaattca tgcgcgcgtt tacttgctaa aatcactaga gaatattttg aatttacacg
    35761 atctaatagt gagtcaattg aaggttttaa catcataaga ctacatctcc tttaacattt
    35821 taatatattt accgattacg cgatctactc gaaaatgttc actggcaata atgtttttga
    35881 ttcgttcaac tgctagcggc acttcatcat ttacaacagc atagtcgtaa agagccatca
    35941 tttcgatttc ttcttttgca actttcattc gtttttcgat tacttcatct gcatctgttc
    36001 ctcggcctac gatccgtgat tttaattccg ctaaatctgg cggagtcaaa aagatgaata
    36061 caccatcggg cactttttct ttgacttgtt ttgctccttg gacttcaatc tccaagaaaa
    36121 catcttttcc ttcatccaat gtcttgttga catatgacaa aggagttcca taataattcc
    36181 cgacatattc tgcgtattcc agcatctctc cagcttcgat cattgattca aactcttctt
    36241 tgcttcggaa gaaataatca acgccttcga cctccccttc acgcatttta cgggtagtca
    36301 ttgagatcga atactgaaaa tcattatcct cactctcaaa aattgctttt cgtactgtgc
    36361 cttttcctac accggatgga ccagacaaca cgattaataa tccacgctct gacatgacga
    36421 cgtcctttca actagtcttt ttgtacttat cgtttatttt gcactttttt tctgcaactt
    36481 tcaaggttat ctttaattat agctagaaat tggtaaaaaa gctaccctgt accaagaggt
    36541 cgtaaaaaag ctgtcctgca tcaagtaata agaacagcca aacataaaca gcttctcatg
    36601 tttttcgttt ggttggggtt attatcgcag atgcaagctt ttgaacgccg tgtataagag
    36661 gtcgtgaaga agccgattag tttcgagtat gaagaaaaat tttgtgaaaa tagtttctca
    36721 catttttgca taaatttggc ttaataccga gaaaatggtt tttgaacatc acttattcaa
    36781 aataaaaaaa gattgtgaca cgaattttgt cacaaccttt aaatgtattc ctagagcaaa
    36841 gaagctaagc gaagaaatcc aattctgttt atttattgta tttctttgtt cagcatcctt
    36901 gctaaaagcc catattggac aaagtattca ctacacccat gatctcatct tttcgagaac
    36961 gtgctttttc tgtcggatct tctacctctt ctgtgtcaat tcgaacaata aatttccgat
    37021 tcatcgtcac gtaagcaccg tcgttgatca aaataaacag ttcgttctca ttatagtctg
    37081 cacaggcatc tttccatacg tcaaagccgt ttttttcaga agggatatcc cgtttaacca
    37141 cagtttttcc acttaattcg tggaacgtta cgtcataata tttcattatc tctacctctt
    37201 tcgtcattca atattttgta cttgttctct gattttttct aagatcgtct tagactggac
    37261 gacttgattt ttaatttcga ttgcatttga tttggaaccg atcgtgttga tttcccgatt
    37321 catttcttgg atcaggaaat ctaattcccg acctacagga cattgtgctt cgataagttc
    37381 atctagtttt tgtacatgga tcgccagacg atccagttcc tcgtgaatat ctccgcgttc
    37441 caacaagatg gctagctccg tcaacaaccg ttgctcatcg actttcgctc caagaaattc
    37501 ctccagcttt ttttgatacc ttatttgaaa ttccttttca tagatttcta caaaactatc
    37561 gagttttttt agtacttctt tgaattcttc agtattcttt ttcaacacta aagctaaagc
    37621 ttgaccttct ttttctcgac tcgcctcaat agaatcaagt gcgcttttca atgtatctag
    37681 agctaactgt gccaattctt ccatgctctc ttccggtttt tcttctaccg ttacgaagtt
    37741 ttcgtccagc acgaagcgtt ccgccatccg actaattgac acttgatttt gactaccgta
    37801 tcgttgttga attccgtctg tcaattgagt gataagtgaa tcaatcaatt cccagttgat
    37861 gacgatctgt ttttcgtttt tgccaatata agctagattc acaaatactt cgattcgacc
    37921 gcggcttaac tgctgtttaa taagctgacg aaaatcgttt tcaacaaagt tcaggatttt
    37981 aggacttcgt atttgaacat ctagaaaacg ctggttgaca ctttttattt caatatctaa
    38041 ctgatacgct tttgtttcgc ggatggcttt gccaaaacca gtcatacttt tcattcgaca
    38101 gggtccttcc tcattacata ttttctattg ctttttgcag ttgttcaaac tcttcgttcg
    38161 tcaatgtcag gcctttcccc attttttcat ggtctggcgc ccaatctcga agatcgaatt
    38221 ttggcgggcg accattccag ctgactaggt tcagttcttt acgccacccc ttgttgtttt
    38281 ctgacaatat agcgatttct tctacgattt catatgaaaa ttcttgtgcc atggttgttc
    38341 ctccttgaga taatataaat ataggatcag taatgtgtca actggttgct taagcaattc
    38401 atgctgcaaa cgatccttgc gttttccttg tagcagataa tcaaaataaa cagttttcgt
    38461 gcgctcattt tgtatttttt gctggtattt tcgatacctt cgccattctt cagtacttaa
    38521 ttgaccaatt tgtgccaata aatcatccgg catacttttt atatcttgtt ggcagatcat
    38581 ttgaatcatc atgtctactt gatagatttc attgggcagt aaaaaatgtt tttttgctaa
    38641 aagttctagc atcgccaatc cgcatgtctc tggtacgacc ctgttcttat ataaattacg
    38701 cagtttttgc caaaatttta tgcttttgaa aaaagataat tctaactgaa tttcttttgt
    38761 taagtaagac aaaaatcctc gccagtatgt tcttgcaggt ttaacagaat gaaatgtata
    38821 ccagttccca tagtaatagc ctaatcgttt tttcatttcc agatagccta attgtaaatt
    38881 taattggttc ctttggctat caaaaagcag aaagctgcct agtgtccaca atgtttgaca
    38941 tttccaatgg ataaaaatgt ctggcatacg aatttttttc gctgggcctg gcccttgcac
    39001 atctacgaca aatgcctctg ttgctccttt gttaatcgca atatcaatag gtattttgtt
    39061 gcgataccca ccgtctgcat atttattttt tccgatcttt cgataagcca tagcgggata
    39121 aaatgaagca gaggcgagta tccaatctgg tatttcttct gtttttgtct gttggatatc
    39181 agtcactact tccgctagtt ctggcagttt agtagacacc gtatataaac gtattttggg
    39241 attatgtttg agtaaggaca aatccaaatt gtctttgacc atcttttcca atggagcaga
    39301 agaaaccccg ccttcaatta gtgctgtttt cgtcatttgg cgtctttctc gatagaagcg
    39361 ttcacgaagg tcttcaactg cagccatttc tggtaacgca agcacttgtc ttgtcgacag
    39421 ctttttccat aaattcacag cttttttcat atctccttgt aaaatcaatg cgccgttcaa
    39481 cgcacctacc gaggtacctg tgataatctc aaagttgata tcatgttcct tcagcgcctg
    39541 ccacacacca atctgataag caccgtgcgc tcctcctcca cctaaaacaa gtgctgtatg
    39601 atagaccagc tttttattta attgtgtggt tccttgatag ccgtttttat gaaaaatacg
    39661 caaccattct tctgatagag gagattgaaa taaaaaacaa cagttttctt taataaaaaa
    39721 acgagcgcaa gcttccatat accgaaaaaa ttttttccat tcaaaagttt caccttcaat
    39781 caacagatta ttaaaaataa catctcctct acttttagca atagtaaaat agacggtata
    39841 ttttttcgat actgctttaa aagcacattg cttttttgca gaacgccaaa aagcgtctct
    39901 actttcttga tgactttgaa aaaatggaag gggttgtttt gcttgctgga taggcgaata
    39961 cagcgacatt ttctgtgaaa agtaaacttt ttttatctcc atcgcgctct cctttttctt
    40021 caagtatacc agtacgtcat aaaaaggtca tgagtctcgt attcgacttt gtagattcca
    40081 taagaaaaca attcataaaa cctcctttaa ataaaaaaat acaaaaatca caaaaattaa
    40141 ttgaaaatga gagtgttttc aatgaaaacg tgattttgta atggaatttt aaaataaaag
    40201 aaacaagctt ttttaatgca aatgttgtaa aataaaagag attaagagga aataagagga
    40261 ggaacatgat gactgagtca gtttatgtac atattgatac cacaagtaat gctgttttaa
    40321 caaaagggct gactgctact gactttgcaa acagtatcgt gcattttcct caaaacttgc
    40381 tcttgcttga tccatctgct tctgctggag aatatgaatc ccatacaggg ctgaaagtaa
    40441 tccgtggtac agaaaatatt cagcgtttct tttcctcttc tcgtaacagg cgtggattcg
    40501 atgaattgaa gtggatcgac tttactgatt taacgatgct gaaagaattg acacctttag
    40561 aaatttcaga attattatat tttggtcata tgaaaacaca tcttcattcg ccgttttttt
    40621 ataaactaca gaataatttt gtatacttcg atcttaatga tcagctgaat cggatttatt
    40681 atcgctattt agatgaattt tataggattt ttgctagtaa attaagttcg atcttgtcag
    40741 ataagatcaa tgtaaaaaaa tcttttttca aaaaagaaac acctgttgaa aaaatcagtg
    40801 tggaattatt gaaagaaatg cgtaatttga tgcaggaagg gatcattttt tcttttgatc
    40861 aagttggttt gatcgatggt aaatatcgta tcccaatcta tgtgattgaa gattatattt
    40921 ggaaattaaa aggcacccgg tatcgagaag aggaaatcat cgctacttta atctactctg
    40981 tcaccgatcg acactggcaa ttggtagaag atgccgatct ttcttatctg ccattgaagt
    41041 agatctattt cttatcaaaa aataaaaagg aaacaagaaa ctaggacatg attctgtctc
    41101 aagtttcttg cttctttttt atttacagct cctgtcgttt atctggcaca aaaatcgaag
    41161 gagtcgtttg ttggatccct aataaaacga tgatcgttac aatagctgta ctcaacccgc
    41221 tggcaccatt cattacgaaa gaaaagagcc aaggattcat cccccatagt gcatacgctc
    41281 cccagaaaac cactcctgca ataaaatgcc aaaaatatct ggaaagtgcg ccaatcaatg
    41341 caccaaacac gatgcttttt ctaatttgtt tcgtattatc gtctacatag cctttttgga
    41401 catttgaagc catgagccct gctgcgcctg caaaagtata ggcaatcggg tactcaatca
    41461 acacttgaac gacacttaaa taataaactt gtcccaacgg aaaatgtagg atgccccaaa
    41521 tgaaacctgc cagcatccct gcctttgtcc ctcttcttaa cgcaaaaaga gtcaatggaa
    41581 tctgccctaa tgaaatagaa aaactgctgc cgatctggat cggaatcaac gacaacacca
    41641 tcgctaacgc tgcaacaatc gttccttcta cccaaactct attttttccc ataaaaaaac
    41701 ttcctccttt gaataaaaaa gtcacaaagg aggaagcagc tttcgaaaat agtcaaacag
    41761 ctgtttattc acaattccta cgctcgcata accgatacag gtacgaaggg tttagaaata
    41821 gattcaatct cagcccaatt ggactcccct ttgtgagttt atttattatt tattttttgt
    41881 ttcaaacaat ggactaactg gtttgttttc atggatacgt ttgattgcat ctcccattag
    41941 tccgccaaca cttacttcat cgattttgtc gattttacga tcttctggaa gatagatcga
    42001 gtctgtaaca actagacgct cgatagctga atcttctatt ctttgtaaag cagggccgga
    42061 taatactgga tgtgtacatg aagcatacac actagttgca ccagcttctt tcaaagcatt
    42121 ggcagccaat gtgatcgtac ctgctgtatc gatcatatca tcgatcagta cacaagtttt
    42181 gcctttgact tgtccaatga tattcattac ttctgcaaca tttgcttttg gacggcgttt
    42241 gtctatgatc gcaatcggag atttcaaaaa ttccgctaat ttacgtgccc gtgtgacacc
    42301 accatggtct ggtgaaacaa cgacaacatc atcgcctttg attccttttt ctaagaaata
    42361 attggcaata agtggcgcac ccatcaaatg atcgactgga atatcaaaga aaccttggat
    42421 ttggactgca tgaaggtcca aagtcagcat tcttgttgca ccagcttttt gaatcatgtt
    42481 tgctactaat ttcgctgtga ttggttcccg tgcacgtgct ttacggtctt gacgcgcata
    42541 gccataataa ggcatgacta cgttgattgt ttttgcactt gctcgtttca gtgcgtcaat
    42601 catgatcagc aactccatca agttatcatt gactgggctg ctcgttgatt gaataacata
    42661 gacatgcgat ccacgaatac tttcttcaat gtttacttga atttctccat cactaaattg
    42721 agtaacagat gattttccta actcgacgcc gactgctgct gcaattttct ctgctaatgg
    42781 gcgattggaa tttaacgcaa aaatttttaa tcttgggtca aagtaatgtt ttgacatggg
    42841 gacctccact ttctttttca caactctaga attctaccta ataaatagta gtcatatttt
    42901 ttaaataaat caagtgattt tgcaaaagtc ttagtttaga taagggagtt tttttgcata
    42961 tccttcttta ttgacttgtc ttgctctcgc aattgccagc gcatattctg gaatattgtc
    43021 tgtaattgtt gacccggcag caatcgaact gtttttagct acctcgacag gtccgatgat
    43081 attcgtgcta gaaccaataa agctgtggtc gccgacagtc gtatgatgtt tgttctttcc
    43141 atcatagttg acgaagacaa caccacatcc gacattgatt tcttctccca gcgtagcatc
    43201 accaacataa gtcaagtgac cgactttagt tcctttgccg atttgagcat ttttcacttc
    43261 cacaaagttt ccgatatgga cgttttcgcc aatttcagct tttggtcgta aatgtgcata
    43321 aggtcccaca tctgcatgtt tcttgacatg gctgctttca atgactgagt tttcaatgac
    43381 gacgtgatcc tcaatcctac tgtctacgat ttttgaatgt gacccgatga cacaatcaga
    43441 accaatgact gtatttcctt ggatttgtac acctgcttcg atcaatgtgt ctggaccgat
    43501 ttctacgcct gcatcgatgt atgtcgttgc tgaatcgaca aagcttacgc cattgaccat
    43561 atgcatctgg ttaattcgct ttctcatgat ctcatttgct ttagctaacg cgatccgatc
    43621 attgactccc attgattctt caaaatcatc cgtttgataa gcagcaactg tatggccttc
    43681 ttcttttaag atttcgatga tatctgttag atagtattca ccttgagcat tgtttgttcc
    43741 aactttgctt aacgcatcga acaaagcttc gttgtcaaaa cagtaagttc ctgtattgat
    43801 ttcttgtaca agagcttctt ctggtgttgc atctttttgt tccacgattt tttctacgat
    43861 cccaatatgg tcacggatga ttcgtccata tcctgttggg ttttctgctt gtgccgtcaa
    43921 gatcgttgca cttgcatttt ttccttggtg atattcaaaa agattgttca gcgtttcagt
    43981 agtcaataat ggtgtatcgc cactgataac taacgttgtt cctttttttc cttccaagaa
    44041 agaagcagct tgcaaaacag catgtcctgt tcccaattgt tctgcttgta acgcatattt
    44101 actgcgttct cctagttgtg ctttaacttg ttctgctccg tgtccaacga tcgtaatcac
    44161 ttcatctggt ttcgtttcac tgacccgatt aatgatatgt tctaccatag gtttaccaga
    44221 aacgggatgt aaaactttat ataattttga cttcatgcgg gtacctttcc ctgccgccaa
    44281 aataattgca taacgtgcgt ccaactgcgt cactcctaaa tatagtttgc tgatttcagt
    44341 ttatcccagt atacccattt tttcaacaaa acaagtgatt ttcttttatc ccagcgtttt
    44401 ctggataaaa aaatagatag accataacgg gcctatctat ttggtaaccg gtgctctcct
    44461 gccaactcct cggtcacaag tcacactact cataaaacat gaaaagctgt tttttgaaat
    44521 cgtttcttgt gacttagagt taaacggtag gcttacaacc tcttcattaa acgtttttca
    44581 atcagctgac cttcggtatt agctcaataa acggcaaaat atgaggagca atttttccgt
    44641 ttattgagct aatactcagg acaggacgct gatttcacaa cctctagata aacggtgttc
    44701 aaaagccagt ttctcggtat taagccaaat ttatgaaaaa atgtggaaag ccattttcac
    44761 aaaatttttc ttactactcg aaactaagcg gctttttcac aacctcttat tctgaaaaat
    44821 aatttcctgg cacaaccgta atatttttcg tttgagtatc tacatctttg acataaagca
    44881 gtgacgtata atcatcgatg gcgcgatgac cgttgaattt tgattcggca aatacagtga
    44941 tgccaacaag ttctgattcg aattcttcaa tcaagctctt cattccgttg actgttccgc
    45001 cgcctttcat gaaatcatcc actacaagga cacgtgaacc acgtttcaaa cttctcttag
    45061 acagttccat cttttcaatt ctttcagaag atccagacac atagttgaca cttactgttg
    45121 atccttctgt tatttttgag tcccggcgta caataacgaa tggcgcattc agataatagg
    45181 acacagcttg ggcaataggt acgccttttg tggctacagt cattacagca tcgatttttt
    45241 caccaaggta tttagaagca atgattcttc caacttgacg cagcagttca ggttctccta
    45301 acaaatcaga gagataaaca tatcctcctg gaagcagacg gtcttgttct gatagacgtt
    45361 ctgctagaga ttcaatatat tttttcgcct cttcatatga aatttcaggg atgaacaata
    45421 caccacctgc agcacccggg attgtttcca aggttccata ccctcgttct ttaaatgttt
    45481 tcttgatgat tcccaaatct tcactgatag aagatttagc agattcataa cgttcggcaa
    45541 agcttgttaa ggaaatcaat tcatgcgggt gattcaataa atactgtgtc atgtcaacta
    45601 gacgttcact gcgacgtact ttcacctatt ccaactcctt ttaagaaatt ttatcgtttt
    45661 acattatata agtttttctt caaaaattcg agcattttat gaaaaaataa gaaaatagtt
    45721 cggtatttat taaaataaaa agaagcctat aa
//
LOCUS       DAZKKW010000014        44940 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.014, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000014 DAZKKW010000000
VERSION     DAZKKW010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 44940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44940
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.014"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            212..604
                     /locus_tag="ACG8PO_000747"
     CDS             212..604
                     /locus_tag="ACG8PO_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFQ0206098.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
     gene            632..1972
                     /gene="glnA"
                     /locus_tag="ACG8PO_000748"
     CDS             632..1972
                     /gene="glnA"
                     /locus_tag="ACG8PO_000748"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HFQ0206099.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            2698..2943
                     /locus_tag="ACG8PO_000749"
     CDS             2698..2943
                     /locus_tag="ACG8PO_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0206100.1"
                     /translation="MKEKDCFYDKLYKLLLQVGKSSSLLGGILSIIMFVLFFYLLFHL
                     KFFSNDFLICLGLTLAMFISSLLCGLNFFCTHKGLKK"
     gene            3165..4775
                     /locus_tag="ACG8PO_000750"
     CDS             3165..4775
                     /locus_tag="ACG8PO_000750"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HFQ0206101.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            complement(4752..4964)
                     /locus_tag="ACG8PO_000751"
     CDS             complement(4752..4964)
                     /locus_tag="ACG8PO_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0206102.1"
                     /translation="MKMKIFKKFFAKNKISNSALIEKSLLLSIFFPTNNNKSEKEKSD
                     KKIAYPLLLLLFSFYQVQKLFCFVIC"
     gene            5067..5936
                     /locus_tag="ACG8PO_000752"
     CDS             5067..5936
                     /locus_tag="ACG8PO_000752"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFQ0206103.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            6101..7372
                     /locus_tag="ACG8PO_000753"
     CDS             6101..7372
                     /locus_tag="ACG8PO_000753"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFQ0206104.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLITTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            7369..8664
                     /gene="rho"
                     /locus_tag="ACG8PO_000754"
     CDS             7369..8664
                     /gene="rho"
                     /locus_tag="ACG8PO_000754"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFQ0206105.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            8763..9026
                     /locus_tag="ACG8PO_000755"
     CDS             8763..9026
                     /locus_tag="ACG8PO_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFQ0206106.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            9471..9920
                     /locus_tag="ACG8PO_000756"
     CDS             9471..9920
                     /locus_tag="ACG8PO_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFQ0206107.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            9977..11395
                     /locus_tag="ACG8PO_000757"
     CDS             9977..11395
                     /locus_tag="ACG8PO_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFQ0206108.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            11395..12255
                     /locus_tag="ACG8PO_000758"
     CDS             11395..12255
                     /locus_tag="ACG8PO_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFQ0206109.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            12429..13202
                     /locus_tag="ACG8PO_000759"
     CDS             12429..13202
                     /locus_tag="ACG8PO_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFQ0206110.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            13349..13714
                     /locus_tag="ACG8PO_000760"
     CDS             13349..13714
                     /locus_tag="ACG8PO_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HFQ0206111.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(13862..15070)
                     /locus_tag="ACG8PO_000761"
     CDS             complement(13862..15070)
                     /locus_tag="ACG8PO_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0206112.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(15089..15367)
                     /locus_tag="ACG8PO_000762"
     CDS             complement(15089..15367)
                     /locus_tag="ACG8PO_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0206113.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            15560..16216
                     /locus_tag="ACG8PO_000763"
     CDS             15560..16216
                     /locus_tag="ACG8PO_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFQ0206114.1"
                     /translation="MDSRVLKEYLDKNDFPIIRKGYHKYLTFEGMEDSHTYILKEGTV
                     KASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESEYAEFYRINRVRFW
                     QNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLYELYDLFGVRTEEG
                     MLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIVILNMDLLEDNIIF
                     "
     gene            complement(16658..17455)
                     /locus_tag="ACG8PO_000764"
     CDS             complement(16658..17455)
                     /locus_tag="ACG8PO_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HFQ0206115.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(17452..18636)
                     /locus_tag="ACG8PO_000765"
     CDS             complement(17452..18636)
                     /locus_tag="ACG8PO_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HFQ0206116.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(18661..19908)
                     /locus_tag="ACG8PO_000766"
     CDS             complement(18661..19908)
                     /locus_tag="ACG8PO_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFQ0206117.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            20123..21868
                     /gene="dnaX"
                     /locus_tag="ACG8PO_000767"
     CDS             20123..21868
                     /gene="dnaX"
                     /locus_tag="ACG8PO_000767"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFQ0206118.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            21888..22202
                     /locus_tag="ACG8PO_000768"
     CDS             21888..22202
                     /locus_tag="ACG8PO_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFQ0206119.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            22227..22823
                     /gene="recR"
                     /locus_tag="ACG8PO_000769"
     CDS             22227..22823
                     /gene="recR"
                     /locus_tag="ACG8PO_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFQ0206120.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            22887..23531
                     /gene="tmk"
                     /locus_tag="ACG8PO_000770"
     CDS             22887..23531
                     /gene="tmk"
                     /locus_tag="ACG8PO_000770"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFQ0206121.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            23546..23875
                     /locus_tag="ACG8PO_000771"
     CDS             23546..23875
                     /locus_tag="ACG8PO_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HFQ0206122.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            23889..24827
                     /gene="holB"
                     /locus_tag="ACG8PO_000772"
     CDS             23889..24827
                     /gene="holB"
                     /locus_tag="ACG8PO_000772"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFQ0206123.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            24863..25687
                     /locus_tag="ACG8PO_000773"
     CDS             24863..25687
                     /locus_tag="ACG8PO_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HFQ0206124.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            25680..26027
                     /locus_tag="ACG8PO_000774"
     CDS             25680..26027
                     /locus_tag="ACG8PO_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HFQ0206125.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            26096..26968
                     /gene="rsmI"
                     /locus_tag="ACG8PO_000775"
     CDS             26096..26968
                     /gene="rsmI"
                     /locus_tag="ACG8PO_000775"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFQ0206126.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            27077..28180
                     /gene="dinB"
                     /locus_tag="ACG8PO_000776"
     CDS             27077..28180
                     /gene="dinB"
                     /locus_tag="ACG8PO_000776"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFQ0206127.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPL"
     gene            complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="ACG8PO_000777"
     CDS             complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="ACG8PO_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HFQ0206128.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="ACG8PO_000778"
     CDS             complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="ACG8PO_000778"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HFQ0206129.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            31684..32451
                     /locus_tag="ACG8PO_000779"
     CDS             31684..32451
                     /locus_tag="ACG8PO_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0206130.1"
                     /translation="MEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGEFVGIMGPSG
                     AGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQEFNLIDTLN
                     AKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQRIAAARALV
                     VQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTASYCDRILFIK
                     DGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            32441..34483
                     /locus_tag="ACG8PO_000780"
     CDS             32441..34483
                     /locus_tag="ACG8PO_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0206131.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
     gene            34764..34913
                     /locus_tag="ACG8PO_000781"
     CDS             34764..34913
                     /locus_tag="ACG8PO_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HFQ0206132.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            34924..36438
                     /gene="dltA"
                     /locus_tag="ACG8PO_000782"
     CDS             34924..36438
                     /gene="dltA"
                     /locus_tag="ACG8PO_000782"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047473 - D-alanine [D-alanyl carrier
                     protein] ligase activity [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HFQ0206133.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            36435..37640
                     /gene="dltB"
                     /locus_tag="ACG8PO_000783"
     CDS             36435..37640
                     /gene="dltB"
                     /locus_tag="ACG8PO_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070395 - lipoteichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HFQ0206134.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            37685..37918
                     /gene="dltC"
                     /locus_tag="ACG8PO_000784"
     CDS             37685..37918
                     /gene="dltC"
                     /locus_tag="ACG8PO_000784"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036370 - D-alanyl carrier activity
                     [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HFQ0206135.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            37921..39189
                     /gene="dltD"
                     /locus_tag="ACG8PO_000785"
     CDS             37921..39189
                     /gene="dltD"
                     /locus_tag="ACG8PO_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070394 - lipoteichoic acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HFQ0206136.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            39330..40412
                     /locus_tag="ACG8PO_000786"
     CDS             39330..40412
                     /locus_tag="ACG8PO_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HFQ0206137.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            40412..41215
                     /locus_tag="ACG8PO_000787"
     CDS             40412..41215
                     /locus_tag="ACG8PO_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HFQ0206138.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            41285..42025
                     /locus_tag="ACG8PO_000788"
     CDS             41285..42025
                     /locus_tag="ACG8PO_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFQ0206139.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            42036..43040
                     /locus_tag="ACG8PO_000789"
     CDS             42036..43040
                     /locus_tag="ACG8PO_000789"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017118 - lipoyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HFQ0206140.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(43085..44929)
                     /locus_tag="ACG8PO_000790"
     CDS             complement(43085..44929)
                     /locus_tag="ACG8PO_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0206141.1"
                     /translation="MSFFNNIYNKKESFYLRIKWSIIIILSLLFLTGIAYFFYFIFSP
                     ADSNQMKEIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLEKPLDEANLL
                     IKGNHQWITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTLTVSSPYQNY
                     AGLPPKVYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMYKQKRLEYSL
                     ILLSCFALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYYWSRMFYYKK
                     WYGIFCTFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLACIGEAYKNNR
                     FYVACTPWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISYNLIDHILNT
                     DKDRRQVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLMHTDQTKAKN
                     YVEKILEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLPEELPINDDD
                     LAQLLIHALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYDTNLFSRGIT
                     EELDDQERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
BASE COUNT        14453 a         7837 c         9348 g        13302 t
ORIGIN      
        1 aataaaaaca ctaataaagg atagtacagt ataataaaaa ataaaaggta tctggtacga
       61 aaatcaacta gatacctttt ttgtgttctt atttttttgt tattttataa aaaatagaga
      121 tctcatgtta gaaaacctaa catatgcagt tgacaatatt ttttttaatt ggtacacttt
      181 agccaactta taaaacgaaa ggggacgtct gatgggagaa aaggaattgc gccgatctat
      241 gtccgtattt ccaattggta cagttatgaa gctgactgat ttgtctgctc ggcaaattcg
      301 ctattatgaa gaacaagatt tagttcatcc ggaacgaagc gaaggaaacc gacgtatgta
      361 ttcgctgaat gatattgata tgttgcttga gattaaagac tatctttcag agggattgaa
      421 tatggctggg atcaaacgtg tatacgaaat ggcacaggcg aaaaaacaag aagcaaaaaa
      481 caagaagccg ttgacagatc atgacgttcg acaaattttt cgtgatgaga ttttggcgca
      541 aggtgggtta agcaaaacag gtcattatca gtcgcaagga ttgcaaagac aattctttga
      601 ctgaataaat taaaaaagga agagggatac catggtaaag aaacagataa caggtgaaga
      661 aatcaaacga atcattgagg aagaaaacgt acgattttta cgattgatgt tcacagacat
      721 cttaggtacg atcaaaaatg tagaagttcc agtgagccag atcgacaaag tattagaaaa
      781 taaaatgatg tttgacggtt cttcgattga aggatttgtt cgaattgaag aaagcgatat
      841 gtatttgtat ccagatctct ccacttggat gatcttcccg tgggaaagtg ctcatggaaa
      901 agttgctcgt ttaatttgtg atatctataa tccggatggg acaccatttg ccggcgatcc
      961 tcgcgggaat ttgaaacgag cattgaaaga tatgcgcgac cttggtttta cctctttcaa
     1021 tttaggacct gaaccagaat tcttcttatt taaattagac gaagacggtg ggatcacaac
     1081 gaccttgaat gataaaggtg gttattttga ttttgcacca acagatttag gtgaaaattg
     1141 ccgccgtgac atcgtgttgg aattagaaag tctaggcttt gaagtggaag cttcacatca
     1201 tgaagtagcg ccaggacagc atgaaattga ctttaaatat gcagatgtgg tagatgcttg
     1261 tgataatatc caaaccttca agctcgttgt caaaacgatt gcacgcaagc atggacttca
     1321 cgcgactttc atgccgaaac cgttatttgg gatcaatggt tctggtatgc attgcaatat
     1381 gtctttgttc aatgatgaag gaaatgtttt ctatgataaa gacggtgaat tggaactaag
     1441 tgaaacagct tatcatttct taggtggttt gctgaaacat gctcgtgcct acacagccgt
     1501 ttgcaatcca acagtcaatt cttataaacg tctcgttcca ggttatgaag cgcctgttta
     1561 tgtcgcatgg agtggccgta atcgttcgcc attagtacgt gtaccagaat ctcgcggttt
     1621 atccacacgt ttggaattgc ggtcagttga tccatctgca aacccatact tggcaatggc
     1681 agtattgttg caagcaggac tagacggtat ccgtaatcag atcacaccgc cagcagctgt
     1741 tgaccgtaac atttatgtaa tggatgagga agaaagagaa gctgcacata tccaggactt
     1801 accttcaact atccataatg ctatcaaaga attgcgtaag gataatgtaa tgattgacgc
     1861 attaggacaa catatcttct caaattttgt tgaagcaaaa cgtctcgaat gggcggcatt
     1921 cagacaaact gtttccgagt gggaaagaga acaatatcta gaactttatt aaaacaggaa
     1981 taccgtgaat tagaggctgt gaaatcagcg ttttgacctg agtattagag aaactaacgg
     2041 aaaaatagtt cctcatattt tgccgttttt tgagctaata ccgagggtca gctgattgaa
     2101 caccgtttag tggagaggtt gtgaaaaagc tgccctgcaa caagtaataa gaatagccaa
     2161 gcaaaaacag cttttcttgt ttttgaattg gctgaactta ttaccgcaga tgcaagcttt
     2221 tgaacaccgt ttaccaagag gttttgaaaa agacgtttag tttcgagtat taagaaaaat
     2281 tttgtgaaaa tggctttcta catttttgca taaatttggc ttaataccga gaaactggct
     2341 tttgaatacc gtttatccag aggttgtgag cctaccattt aactccgaat cacaagaaat
     2401 aatttcaaaa acagcttctc atgtttgatg gatattgtga cttgtgaccg aggagttggc
     2461 aggagaacac cgtttattta gaaataagga ggcacgactt ttgtcgcatc tccttatttt
     2521 ttacttatgt gattcgttac cttcaatgaa tccctacaag gcacttttgt tgaaaaaaag
     2581 gtcatctttg aaaaaattag tggaattgtt ttgtttttga tattatttaa tagaaaagat
     2641 atgccaaaaa tccgagaact gtggactacg ttttttacgt aaaggaggta aataagggtg
     2701 aaagaaaaag attgtttcta cgataagcta tataaactgt tgctgcaagt aggaaaaagt
     2761 agttctttgt taggtgggat attatctatc ataatgtttg ttttattttt ttatctatta
     2821 tttcacttaa aatttttcag taacgatttt ctgatttgct taggtttaac gttagctatg
     2881 tttatatcaa gcttattatg tgggttaaat tttttttgca ctcataaagg acttaagaaa
     2941 taatttctca aaggatatcg aaaaagaaaa aattgtaagc ggatagtatt catttgagta
     3001 cttgactttc ccttagtcag cttgtattat acaccttggg caccctttta gagggtcaga
     3061 agtttacaga aatttcggct ccctattctt attgaagaat agggagcttt gtttattgtc
     3121 tgagactttt cctgcacata aattaaaaag gagtttttga acttatgaca aaatatattt
     3181 ttgtgaccgg cggtgtcgtt tcatcaatcg gaaaagggat tgttgcagct tctttaggac
     3241 gcttattgaa aaaccgcggc ttaaaagtaa ctatccaaaa atttgatcca tacatcaacg
     3301 tggatccggg gacgatgagc ccatatcagc acggagaagt attcgtaaca gatgatggtg
     3361 ctgaaacaga tttggatctg ggccactacg aacgttttat tgacatcaat ttgaacaaat
     3421 actccaacgt gacgacagga aaaatctatt ctgaagtttt gcgtaaagaa cgtaagggcg
     3481 aatacttggg agcaactgtt caagttattc ctcatattac aaatgagatc aaagaaaaaa
     3541 tcatgcgagc agctaaaatg actgattcag atgtgatcat tacagaagta gggggaacag
     3601 taggggatat agaatctttg ccattccttg aagcacttcg tcaaatgaaa gcagatgtcg
     3661 gtacagataa tgttatgtat atccatacaa cattgattcc ttacttgaaa gctgctggag
     3721 aaatgaaaac taaaccaacg cagcacagtg taaaagagct acgtggttta ggtatccaac
     3781 cgaatatttt ggtggtacgt acagagcaac ctgtttctca aagcgtgaaa aacaaattgg
     3841 cacaattttg tgatgtcgaa ccagaagcag ttatcgagtc gccagatgta gatacacttt
     3901 actccatccc attagtttta caagcacaag gaatggatca aattgtttgc gaccatttaa
     3961 aactagatac accagaagca gacatgacag aatggaaaga attagaagaa cgtgtcttga
     4021 acttgaagaa aaaagtacgg attgcgcttg tcggtaaata tgtcgagtta ccagatgcat
     4081 atatctcagt agttgaagca ttgaaacatt ctggtttcgc ctataattca gacatcgaga
     4141 tcgattggat caaagcacaa gaattaacga gagaaaatgt agaagaacgt ttgaaagacg
     4201 cagatggaat cttggttcct ggtggatttg gtgatcgcgg agtagaaggg aaaatcgaag
     4261 cgattcgtta tgctcgcgaa aatgacgttc cgtttttagg tatttgtctt ggtatgcaga
     4321 tggcttgtgt cgaatttgca cgtaacgtcg ttggattaga agatgcgtca tcagctgaaa
     4381 cgaatccaga tacagcaaac aatattattg atttaatggc tgatcaggaa aacatcgaga
     4441 atttgggtgg aactttacgc cttggattat acccatgtaa aatcaaaaaa ggaacgaaga
     4501 cggctgaagc atatgatgga gccgatgttg tgcaagaacg tcaccgtcac cgctatgagt
     4561 tcaataacaa atatcgtcaa ttgtttgaag aacaagggct agtcttttca ggtgtctcac
     4621 cagataaccg tttagtagaa atcgtagagc tgccagagaa aaaattcttt gtgggatgcc
     4681 aattccaccc agaattgatt tctcgtccaa atcgcccgca aaaattaatc aaagcatttg
     4741 tcggagcatc attagcagat cacgaaacaa aataattttt gaacttgata gaaagagaaa
     4801 agtagcaaga gaaggggata agcaattttc ttgtcacttt tctctttttc tgatttgtta
     4861 ttatttgtag gaaaaaagat agaaagcaaa agactttttt cgattaaagc gctattggat
     4921 attttatttt ttgcaaaaaa cttcttgaaa attttcattt tcattgattt tagtagaaaa
     4981 ctaccgaaat gtttggtaaa atagggtcgt tggttaagaa ttgtcttaac aaatttagtt
     5041 ttacaaaact taggaggaat tttattatgc cagtagtatc aggagctgaa tttttaaaag
     5101 ctgctcgtaa aggcggttac gctgtcggcg gattcaacac aaacaacttg gaatggactc
     5161 aagcaatcct tgaagcggct gaagcaaaac aagcaccagt acttatccaa acatcaatgg
     5221 gtgcagctaa atacatgggt ggttacaaaa tctgtaaaga tttaatcact gatttagttg
     5281 aagcaatgaa catcactgtt ccagttgcta tccacttaga ccatggtgat tacgaagctg
     5341 ctttggaatg tatcgaatta ggctatactt cagtaatgtt cgatggttca catctaccat
     5401 ttgaagaaaa cttgaaatta gctaaagacg ttgttgaaaa agcacatgca aaaggtatct
     5461 cagttgaatg tgaagttggt tcaatcggcg gagaagaaga cggaatcatc ggtaacggcg
     5521 aattagctga tattgaagaa tgcaaacaaa tggttgctac aggtatcgac tacttagctt
     5581 gcggtatcgg taacatccat ggtcaatatc cagaaaactg gaaaggatta gcttttgacc
     5641 acttacaagc aattgctgat gctgtaggtc cagacatgcc tttagtatta catggcggat
     5701 caggtattcc tcaagatcaa atcgaaaaag ctatctcaat gggtatctca aaagtaaatg
     5761 ttaacactga attccaatta tcatttgctg cagctactcg taaatatatc gaagctggta
     5821 aagataaaga aggtaaagga ttcgaccctc gtaaattatt agctccaggt aaagcagcaa
     5881 tcgttgaaga tgctaaatct catatcgact ggttcggttc agctaacaaa gcgtaagcat
     5941 ccacataaca gatacgatat ataggaatgg agaacgactt ttgaagtcgc tctccattct
     6001 tttttactta aaacggcaga ccatgctcgt ttcttttttt tatctatgat aaaatacaag
     6061 catatccatg aaaaaagatt aataaacaag gtggaaacag atgaaaaaaa tcgtaatcaa
     6121 aggaaaccga cctttagctg gtgaagtaac gatcagcggt gcaaaaaata gtgcagtcgc
     6181 attgattcca gctgcaatct tagctgattc cccagtgatc ttggaaggtg tgccggacat
     6241 acaagatgtt cattcattaa ttgaaatttt agagatcatg ggcgcaactg tccatttttc
     6301 caacaacata ctagaaatcg atcctcgaga agtcgtatcg attccgatgc cgcacggaaa
     6361 aatcaatagt ttgcgtgctt cttattattt tatgggaaca ctgcttggta aatttggcga
     6421 agcggtagta ggcctgccag gtggctgtta tttaggccct agaccgattg acctacacgt
     6481 caaaggattt gaagctctag gtgctgaagt gacaaatgaa catggtgcca tgtatttacg
     6541 gacagaaaat aaaggattgc gtggcaatcg gattttcatg gacgtggttt cagtcggagc
     6601 aacgatcaat gtgatgttag ctgctgtaaa agcaaaagga cagacagtaa ttgaaaacgc
     6661 tgcacgagaa ccggaaatca tagatgtggc aactttactg aataatatgg gggcaaaagt
     6721 tcgaggagcg ggtacagatg tgatccgtat cgaaggagtc gaaacattac atggctgtcg
     6781 gcatttcatg attcctgacc gcatcgaagc aggaacctat ctagcattag cagctgcagt
     6841 aggaaatggt atcaaagtga aaaatgtgat ctttgaacat ttggaaagct ttatagcaaa
     6901 attgcaggaa attggtgtcc atatgaaaat cagcgaagat gaaatcgaag tttacccctc
     6961 gaaagaatta aaacccgcaa acatcatgac ctatccttat ccaggatttg ccacagatct
     7021 gcagcagcca cttactgcgt tactattgat tactacaggc acttcagaaa tcatcgatac
     7081 gatctatgcg aaacgtgtga accatgtgcc ggaactagca cgaatgggcg ctgatattac
     7141 cgtggaagga aatatgatca tcgtgaatgg tccaaataaa cttcacggca cagaagtcgt
     7201 ggcttctgat cttcgggcag gtgcttgttt agtgattgct ggactattgg cagaagggac
     7261 aacgacgatt tacaatgtag attatatctt acgcggttat gatcatatta tcgagaaact
     7321 aacagcctta ggggcatcaa ttgaaatgat agaggaagtg ggagctgaat gagcgattat
     7381 ttgacaatgg cagaactaga gaacaaaacg ttaaaagaag tttatagtta tgccaaagaa
     7441 tttaaaattc cttattacag taagatgaat aaaaaggaat tatctctagc tgtgattcgt
     7501 gcacaagcag agaaacaagg attttattat atggaaggaa tattggatat cgttggtcaa
     7561 gatggttacg gttttttacg gccaatcaat tatggaccaa gtgcagaaga tatctatatt
     7621 tctgcctcac aaatcagacg attcagtttg agaaatgggg acaaagtggc aggaaaagcc
     7681 cgtccaccta aagaatcgga acgttattat ggtttgatgc acgtggaaag cgttaatgga
     7741 aaagatccgg aagaagcaaa agaacgtccg cattttccag ccttaacacc tctttatccg
     7801 caaagacagc ttcaactgga aacaacaccg caaagactat ctacccgaat gatcgatatc
     7861 ttttctccaa tcggttttgg tcagcgtgga ttgatcgtcg caccgccaaa agcagggaaa
     7921 acttctgtat taaaagaaat cgcaaatggt atcaccgaaa actatccgga agtagaatta
     7981 atcgttctac tgattgatga acgaccggaa gaagtaacgg atctagaacg aagcgtgaaa
     8041 ggcgacgtgg tatcttctac atttgatctg cagccgcaaa accatactcg agtttctgaa
     8101 ttagtattgg aacgcgcaat gcgtttagtc gaagataaac gagatgtcgt tatcttgatg
     8161 gatagtatta ctagacttgc tcgggcctat aacttagtcg ttccaccaag cggacgtaca
     8221 ttgagtggcg ggattgaccc agccgcattg tataaaccta agaaattttt cggggcagca
     8281 agaaacatcg aagaaggtgg cagtttgacg attttagcga ccgctttagt cgatacagga
     8341 agccgtatgg atgacgtgat ctatgaagaa ttcaaaggaa ctggaaatct agaattacaa
     8401 ctttcacgtg aattatctga acgtcggatc ttcccagcca tcgatatcaa aaaatcaggt
     8461 acccgaaaag aagaattatt gatgccaagt gaagaattag aagaaatttg gaaacttcga
     8521 aaaaatatga ctggagattc attagaatat acggaacaac tgatccgctt tttgcgtaaa
     8581 acaaaaaaca atcagcaatt tttcaaagaa tttcaagatg tctcctttgg taaaaaaaat
     8641 ccgaccagaa atctgaaaag ataattgcaa ccttgactga aacatgttaa gatgttaatg
     8701 ttgtaaatcg acaaatcaac tctggttcag caaatggagc agggcaaagg agcgaaaaaa
     8761 tcatgaaaga aaacatccat ccagattacc acccagtagt attcatggat tcaacaactg
     8821 gtttcaaatt cttgtcaggt tcaacaaaaa cttcacaaga aacagttgaa tgggaagacg
     8881 gtaatacata cccagtcatc cgtgtcgaag taacttctga ctcacatcca ttctacactg
     8941 gacgtcaaaa attcacacaa gcagacggac gtgtggaccg tttcaacaaa aaatacggtc
     9001 tcaaagacgc aaacgctgcg gaataataaa aacgctgata actcaacgtt tagcagacta
     9061 tctgataggg atagtctgtt tttttatgtt ttttgccaat ttaaaacttc gtttgatgct
     9121 gaaaaagtcg taggatcata atgcacaaaa caatgaaaaa ctagtatata gttttaatta
     9181 tccacttgat aaaaacacct tatattaacg aaatgaagag gggttatttc gaagtaaaaa
     9241 accatttacc taaaattaga agctaagctt tcctttcaag gaaggctttt tgttttgtta
     9301 tagacttgcg attgtttgtg agctagatcg ttcattttaa tgacttatac acgcggatga
     9361 tgggtctttt tcctctgttc attcataata ttgaacggta cagctagcga tgctatactg
     9421 acctcagttc caagaaaacg ttaacatttt ttgtggggga gaggtttttt atgaggtttg
     9481 atcaattgat cgcagaagaa ctgattgttt tcgatccatc gatagattca aaaggatcat
     9541 tatttgagcg aataggggat gtgttgaaaa aaacaaataa ggtaagaaat gttcaaaaag
     9601 tcgttaaggc actattcaaa cgagaggagg agatctccac aggaatagaa gatggatttg
     9661 ggattcccca tgcaaaaagc aaatatgtga caaaaccagc actgtgtttt tttcatacag
     9721 ccaaattaac ggactatagc gggatagatg aaatgccaat tgaatatatt tttgccatcg
     9781 tagttcctga aaaatcagca gatatgcatc tagagattct cagcacactc tccagacgat
     9841 tgatgaatgc agattttaga aagggaataa aagaagccac tacgccagca gatatcatca
     9901 gacagattag tcaagaatga aaatagtaga gtctttgtcc gagtatcgat agtgaagaaa
     9961 aaggaggaaa ataatcatga aaatcgttgg tgtaactgct tgcccgactg gaattgctca
    10021 tacttatatg tcggcagaaa aattagaagt aacagcaaga aatctaaacc atcaagcaaa
    10081 attcgaaaca caaggtgtaa aaacagaaaa tcagttatct gaacaagaaa tcaaagaggc
    10141 agatgcaatc attcttgcag tagataaaga aatcgaattg gaccgatttg ccgggaaaaa
    10201 agtaaagcgt gtctctacaa gccgagcaat caaagaaccg caagtagtaa tagacgaagc
    10261 attgagagat attggcgtat tcgttgtgtc taatgaaaaa gaaccagctg caaatgagaa
    10321 aaagccgaca atctataatc atttcatgaa tggtgtgaat tacatgcttc catttgtgat
    10381 cgcaggcgga atcatcatcg cactgagttt tgcctttggg attacagcgg ctgatcccaa
    10441 ctcagcagac tataatgtgt tagcagctgc attttcaagg atcggaggag atacagcgtt
    10501 tgccatgatg gttcctgcgt taggcgcggg gattgctaca tcgattgctg gaaaagcagg
    10561 ttttgcccca ggaattgttg ccggattact ggcctctaca ggtggttcag gttttcttgg
    10621 cggaatgatt ggtggcgtgc tagcagggta tatctgtgat tttctggcaa ataaaattag
    10681 cgtaaaaaaa gaattttctg ctatttatca gctgattgtc gtcccctttt tatcgatctt
    10741 aattattggg cttttgatgg tatttgtgat tgaacaaccg atcacatggg tgttggatgc
    10801 tttgacaaat tggctgaata gcttgggaaa tacatccggg ctattgttcg gattacttat
    10861 cgggatcatg atggcagctg atatgggtgg gccgatcaat aaatcgactt cgactttctc
    10921 gatcggtttg atgtcagctg gagtaaatgc accgattgct gcttgtatgg ctgcaggaat
    10981 gacacctccc ctaggtattg ctttagccac tttattattc aaacataaat ttaccaaaga
    11041 agaacggact tcaggtcaat catgctggat tttaggcgct tcgtatatta cagaaggtgc
    11101 tataccgttt gcggtatctg acccgatccg cgtgatacct agtttgatga caggttcagc
    11161 agtcgctgca gggatttcca tggcagtagg agttacttca atggcacctc atggagggat
    11221 ttgggtgatg ctaattccaa atgttatgaa caatttacct atgtatgtct tggcaattgc
    11281 atcaggtaca gtcactacag cctttatgct tggactattg aaaaaaacag tgaaagagaa
    11341 aaaacaagac aagtggacga aaaatgaaaa tctaacaaat gaggtggaag cgtaatgtta
    11401 tatacaatga aagagttatt gaaagtggct aaagagaatc aatttgcagt accagccttc
    11461 aatatttgca gctatgatat gatgaaagca atcatcgaag aagcagagag attagaagca
    11521 ccagtaatct tagaaattca tcctgatgaa atcgcctatt tgagtgatga gtttgttgcg
    11581 gcagtaagag cctatgctta tacaagtaaa ctgccgatcg ttatccactt ggatcatggc
    11641 ggtacgatag aagatgtctt acgggcgatt cgaaacggct attcttccgt gatgatcgat
    11701 gcgtctttga aatcattcga cgacaatgta cagattacga aaaaagtggt tgcgttagct
    11761 catgcggtaa atgtctcagt ggaggctgag cttggaacga ttgggaataa tggttctgct
    11821 gaaggaggtg ctgaaacaat catttataca gatcctgatc aagcgaagat ttttgtgaaa
    11881 gaaacaggaa tcgatacact tgctgtagca attggaacag cgcatggctt gtatccgaaa
    11941 aacagaactc caaaattaaa tatcgactta ctgaaagaat tgaaccaaca aatcgatctt
    12001 ccatttgttt tacatggcgg ttcaggtaat ccggacgcag aagtcagcga agcagtaaaa
    12061 tacggagttg ccaaagtgaa tttgagctca gatcttaaaa gcgtcttttt caatgctttg
    12121 agagaaatat tgagtgaaaa tccatcgatg tacgagccga atgcaattta tccagcagct
    12181 aataaaaagg tacaagaagt ggtcaaacat aaaatggaaa ttttgaacac agtaggcaag
    12241 gctagtttat actaaaaata aaaatgtgtg gtcaagaggg tatgacaata gtcttgtcat
    12301 accctcttaa ccacacacga tgttcagaag tagttttttc gaaaaatttc ttcttatttc
    12361 tcgaagccaa tcacttctgt cacaacctct tttttcctgt tactatataa agtaaatggg
    12421 gtgattttat gttgaatgaa gtggagaata gacagcagga gattgtgaat tatctaaaaa
    12481 acaaccaatt tgcaagagtc accgatttga tcgagttagt aaattatagc gaagccacga
    12541 ttaaacgtga ccttgttttt ctggaaaaaa gtggattgat ccgtagagtt cgcggtggcg
    12601 ccatgcttat tgacaatcga aaaattgacg tgccttacct gatgaagatc accaaattag
    12661 aagaagaaac agaaaaacgt aagattgcag atacagcttc ttctttgatt cgagatgata
    12721 tggtcttatt cttggattcc agtacaacct ctttacactt agtcagaaac ctagcaagat
    12781 ttgatggttt gcagatcatt acgaatggca tcgtcacagc tgctatgttg agtgaatata
    12841 caacggcaaa agtatctgta ttgggagggg caattgttcc gaaacgagca acaatcaacg
    12901 gttcaaaagc ctataacgat gcattgtcat attatgcgga tttagcgata atgagttgtc
    12961 gcgggctaga tttcagccaa ggagtaacag aaactcatga aggagaggca atggtcaaaa
    13021 aagcttttag aaaacaatca gaccggcttg ccgttttagt tacacagcaa aaaattgcac
    13081 ataaattcat gcatcaagcc attgcttttc atgaaatcga ttatctggtg acaagtaaag
    13141 aactgacaaa agaacagtat gcgaaagtaa aggaacacca tatcgaatgt ctgtacggat
    13201 aaattattac gtgaatgttc taagtggctt cctcattttt agataggacc ttatctttgc
    13261 tcgcttctct cagccgatgg tataataatg aaaaggttat cttgttagat aatcttactt
    13321 ttttatccag gagaaaggag tgcagaaaat gaaacatgtg aaggcgttgg tagtgaaggc
    13381 aatcatgatc tgggcgattc tgtgggtcgt gctgacaggt ttatacggtg ttagctttat
    13441 ggattcaacg attgttgggg taattattgt agtcatgatc tatgtgctag gggatctgtt
    13501 gatcttacgt aaagtaggga acatcgcagc gacgatcgca gatgctggtt cagctgcagt
    13561 tatcttgtgg ctctatctat attttatgaa tgatacagtc gatatttgga tgaaagtatt
    13621 gattcctgct ctattgatcg gtgtggcaga atggttcttc cacaagtggt tgctaagtca
    13681 aggcatcgta ccagatgaac ggaaaatgga ataaatcaac agttaaacta ttcttatcga
    13741 taaatgaaag ctttaaaaat aaatagatgt ttaattaaga ataatcataa actattttta
    13801 ctcaaaaaac cgctcagatt attttcagat ctgaacggtt ttttttatct attcatcaag
    13861 atcaaatttc atttttcaat gattcaatca attcgccctc tgcttcatga ctatgttttc
    13921 ggccattaac aagatagagt gctgtacata tcaatggaat gactccggca atcacataca
    13981 atcctctaaa tgaagtaaga gattttagca tccccatcaa ataaggacca acgcctaagc
    14041 ctaggtctaa accaataaag taggtagata aagcaatact gattcgatgt tcgcggacga
    14101 tttttaaaca aacagcctgt ccatttgaca taaacgtacc ataacctagg ccgactaatg
    14161 ctccagagat aagcaatgtc acactattcg tcgttacact cagtaaaaat aatcctgctg
    14221 ttaagaataa atagctagga tacatcacgt attgttctcc tttgacatcg aaaatccttc
    14281 cagtcaatgg acgtgtaaat gtgatgacca aagcatatac gacgaagaaa aatgacccag
    14341 cagcaactaa gtggattact tttgtataag aagataaaaa tgataaaaca cttgaataag
    14401 aaagacccat caaaaaggca attcctgtaa tgaaaagtgc tttcttttca ataaaactat
    14461 tgatattcca tgagcgaaga gatatttttt gttcttcaga taatggaata ttttttacag
    14521 gaaagacaag gcaggcaatc gtaacaaaga agaccagtac aattgaaaac cagataatga
    14581 aacgaaaatc agtcatattc agcaaaatca ttcctaaaaa tggcccaaca gcggcagcta
    14641 aactagtact taatccataa taattgatgc cttccccttt tttagatgca gggatatatg
    14701 cagtaacgat agcatttgta gctgtagaaa ctgtaccgta gccgaatcca ttcaagaatc
    14761 gaactaaata caatgccccg atagttggcg tataaagata tgctaatgtt gaaaggagat
    14821 agaagatcgc gccgcctctt aaggtaaact tccgaccaaa tagttctaac tgtttcccca
    14881 tgaataagcg tgccagcaat gttccgatga tatagatccc agaagcaaaa cctgcttggc
    14941 tcaagctggc gtgtaaggtg tcttgcgcaa tgactgccat gatgaccatc aacagataat
    15001 aaatcaggta gacaataaaa ttgatcaatg tgattccaat aaaatattta ttaaataatt
    15061 tcttttccat gtatgttata cattcccctt aagctgaata tgcttttaat ttttgtattt
    15121 gaacttctac atcgtgataa ttggatggat agcgatcaac aagttggcga ataaataaat
    15181 ctcttgtaga cataaacaga tttaaaaatt cttcgcattc gtttgttgga ttctcatcca
    15241 ctgctttgat caattcgatc gagttttgat acgcatcatt taaaaagact aaagaagctt
    15301 tcatatcaaa tcttgtttga tatgctgttt ctaatcgatt aatgattgtg ttcatccagt
    15361 aattcatgtt ttttccctct attcttaaaa aattttactt ctgcagtata acgaataaaa
    15421 aaaaggaaaa aaatcgcata agtgatggaa aaaggtactt aacaaatgtt aatatcatta
    15481 gtaaaatacc tttggacttt aaaaagtttt aaagtactgt atgatctttt tagatgaaag
    15541 tttggatggg ggagcatcat tggatagtag agtcttaaag gaatatttgg ataaaaatga
    15601 ttttccgatt atacgcaaag gatatcataa atatttgaca ttcgaaggga tggaagattc
    15661 ccatacgtat atcttgaaag agggaacggt aaaagcaagt gttatttcaa atgatgggag
    15721 agaatttaac cttcgctata tcaaagatct tgaaatcgtc tctcttttaa aagacgaata
    15781 ttcacaattc atcgattcgc cgtataatat tcgagttgaa tcagaatatg cagagtttta
    15841 tcgaatcaat cgtgtgcgct tttggcaaaa tatcaaccaa gacccaaagc tacaacacta
    15901 tgtaaaagag tattaccgct atcgtctgct ttattcgatg aaaaaaatgc agcaaatgct
    15961 attaaacgga aagctgggag ctgtttgtac gcaactttac gaattatatg atttattcgg
    16021 tgtgcggaca gaagaaggga tgctgatcga tttttcgatc acgaatgaag aaatcgccaa
    16081 attttgtggc atctcaactg ctagcagtgt aaacagaatt ttaaaacaac tgaaaaaaga
    16141 tggtattatc accgttaaag accatcatat cgttattttg aatatggact tgttagaaga
    16201 caatattatc ttttgatgaa caaagaggtt gtgaaatcag cgttttgacc tgagtattag
    16261 agaaacaaac ggaaaaatag ttcctcatat tttgccgttt tttgagctaa taccgagggt
    16321 cagctgattg aacaccgttt accaagaggt tgtgagcctg ccgtttaact ctgagtcaca
    16381 aggaacaatt tcaaaaaaca gcttttcatg tttgatgaat attgtgactt gtgaccgagg
    16441 agttggtagg agaacaccgt ttaccaagag gttgtgaaaa agctgttctg catcaagtaa
    16501 taagaagagc caagtaaaaa cagtctctta tgtttttaac ttggctggac ttatcaccgt
    16561 agatgtaagc ttttgaacgc cgtttatttg caacaaaagg ggctgtgaca cgatttttgt
    16621 cacagccctt tttttgcgaa cattcattta gcaattttta gaaacttctg ccgccaccgc
    16681 caaaagtccc gccaccgcta gaatgggtag tacttccgcc accgccgctt ccaggaggtg
    16741 ggcttttggg gattcttctt gtcgtgacaa aagaatttgt cagacgatcg gttttttcag
    16801 ttaatttgat actggatttt tctcggaatg gatatttgta gatgcctgat ttcaattgat
    16861 aacgcgaaac agtcacaaaa taaaagacaa gactaaggat aagagccaaa acaacagcaa
    16921 tgatgatttc tactgtcgtc aaaaccttat agcgtgtaat tttgccagtt tcttcgtcta
    16981 cacgataatg tcctcctgga actccgtctt ccacatatgt tgctgattta gacaggtaag
    17041 cttttgcagc attgtaatag gcagcatttg tcatctgatc ataaacatca tctaagatcg
    17101 aatcgattct gctatcagtc aaataatcga tcatattacc agaagtcgaa atgtagattt
    17161 cacgctgtcc catatctaat agcaatactg agccattttg atcatttccg actgcgtctc
    17221 ttaagtaatt atcagcaaag tctcttggtt cttctgaatt agatgtagtc gttacgatca
    17281 agactcgtcc tttgattttt tcattcagct tttctgcctg ttgttccaaa gattggattt
    17341 gatctgttgt gaataaccca gcctcatcat ttaccgtagc tgtatctgca tgagtagcca
    17401 ccggccagcc aacacttact agcaaaagaa agaataaaaa aaattttttc atcatatcaa
    17461 gtaacctccg atcagaaaga ggatcgctag cccagcgaaa atcccaagag cggtcagtcc
    17521 caattttttc tggctgatcg gcaggacacc gctgaccttt cctgtttgtc cattcattgc
    17581 gtagtagtag acttttttac ttgagtcatt gcttcgataa gtaacaagcc ataccggcag
    17641 taatacgtaa ttatttactt cgtttgtgat tgaagcagaa gttcgcacac cggtcaatgt
    17701 tgtatatcca tttgcggtat cttttaacag cttctctgaa tagtctttta attcagattg
    17761 aatctgtgat ttgattgcct catattcgat atctcttttt tctgcttgga acccggctaa
    17821 atactggctt ttaaaatcaa ttgctttatc taacgggaaa ggttgcacgc cttcgaccat
    17881 cttttgttgc gtattttttg ataaggcatt ttttactagt tctttaaaag aaagcttgcc
    17941 tgctcttgaa acagcaaatt gcttcgtttc tgtgtattcg atgtcaccta tccgccatat
    18001 gcggataact gtaccattcg cctgcatggc accatctact tcagcatctg tagcccaata
    18061 agggaaatac acacctgtca acttatcgat ttgatcctta ttgaaaaaag ctttgggaac
    18121 aaaccatttt ttcttggtcc aagcgagaaa tctgttgatt gcttcttctt tctcgacagc
    18181 aaaaggaagt actttatttg gcaagaattc tccgcttaaa cgtcccgata gtacgacggg
    18241 attatggcag tagtaacaat aagtcgcagc agttgtcgca tcagtcacga tctctgcacc
    18301 acagttggga caaagaaaaa gatctatcgc tgcggtatta tctgcagatt cgtccgctgt
    18361 atctgaaaaa ccgcctgcct cgtcaaccgc tttgcgctcg gcttcattca tatcggcaag
    18421 ctgttcttgg gctgtaaaag taaaatcttc ggtaggtgaa ggttcttctt tgctttcttc
    18481 gccagccata cgcgcttctt tttgcttttg ctcatattgg ctgacttctt cttctgtata
    18541 gatattcagg cagtattcgc aatgaaattt ttggtctttg ggatcaaaag tcaacggacc
    18601 gccgcaatta ggacatttat gtgtaagaac atccatcagc tgcacacctt tctaaataaa
    18661 ttaattaacg gggattcctt taaacggttt accacagttt ggacagaatt tagggatgcc
    18721 gttggaaaga tccacgatct cattgcattc actgcatcgc atttgtagct taggctgaac
    18781 gttttctaca ggtttggcag ctccgcagtt ggaacagaat tttccggaat tttctgtccc
    18841 gcaaactgga catgtccaag tatccgcatt tccttgtgca gtttgttgat tttgtttttc
    18901 tgcttgttgt ttgatttgtt cttggttatt ttgagctgct tgagaaagat aagaaccatt
    18961 tgcattcatt cccataccta cacccataaa gccggtcatt gctcctgctt cattttttcc
    19021 ggctgcttcc ataccacgtg caatggaacc ttgcacgtat ccttcacgta cgctaggatc
    19081 acctagcatc gccccttcgt tacgcatatt gatcagtttc acagaatcat cggtgtaaga
    19141 aatgctggct acagccacag aaacaatctc catcccacgt aattcacgcc aagaatcatc
    19201 caatgcagta tccatgtatt tgcttaattc caaactctta gatggtacat aagaaatccg
    19261 ttgaccatct gcagacatct gattgattgc tgattgtagc gctgttagga attcagctaa
    19321 atactgctca ttgatatcat tgatttccac ttgtgttttg tttttaggga ttgcgtttgt
    19381 ataaaataaa atcgggtcaa cgacatgaat agaataagtg ccatgtgccc gtaagaataa
    19441 ttctgcgtta tagaaattgt caaagtagtt caacggcgcg cttgtaccga atttgattcc
    19501 ctttatttct tggagattga tataaaaaac ttgttgtttc tgcggtgtga cgccgccaaa
    19561 tttgaatcgg tcaaaagttt cagcaattgc ttctttcagt gttccattga acatggaagg
    19621 agctgcgtca tttttaatgg tgtaataacc ttcctcagcg gtataatcga tgatttttcc
    19681 accatcgacc aaaagcatca tcatgtttgg atagacatgg acgactgttc cgtctgttaa
    19741 aacatcttct gttcctttac gattggagcc acgtttgtca tctttgcgga ctttgacacc
    19801 ttttgtcatg accgttgtat cactcatatt gtctggttcg atgacttcta gccattgatc
    19861 tgccagaccg ccgccaacca tacttgttgc tgctttgata agacccataa aaaattcctc
    19921 ctctgattca gctagtgcca aacaattatt gtttatttat tataccatag gaagatgtga
    19981 aacttctcgg actagagacg gaattgaaat ggaaaattgc gtattgttta aatacctgaa
    20041 aaagccgaga aggatagtag tttttttacc taatatcacg tataataaga gatagtgaat
    20101 tttttaaaaa agggggatcc ttatggcata tcaagcactt tatcgagtct ggcgttcgca
    20161 gcgtttcgaa gacatcgttg gacaaaaagc tgtgacacag acactaaaaa atgctattgt
    20221 ttcccacaaa acctcacatg cctacttgtt tacaggaccc agaggaaccg gaaagacgag
    20281 tgcagcaaag attttcgcaa aagcaatcaa ctgtccgaac agtaaagacg gtgagccatg
    20341 caacgaatgc gaaatgtgtc gatcaattac tgccggtacg caagaagatg tgatcgaaat
    20401 cgatgctgcc agcaataacg gagtcgaaga aatccgattt atccgtgatc gggcaaatta
    20461 tgcgcctaca aaggcagcgt acaaagtgta cattattgat gaggtacata tgctttcaac
    20521 aggagctttc aatgccttgt tgaaaacatt ggaagagcca aaagaaagtg tcatattcat
    20581 cttagcaacg accgaacctc acaaaatacc agctacgatc atttcacgga ctcagcgatt
    20641 tgattttaaa cggatcaata cggcagatat tgtcgaacat cttgaattta tcttgaaaga
    20701 atcgactatc agctatgaac cagctgcatt gaatgtgatt gcccgttctg cagaaggcgg
    20761 gatgcgggat gcattgagta ttcttgatca ggcaatctcg tttagtgaag gaacaattac
    20821 tttagatgat gccatgcaag tgacaggtag cttaactgac gaaatgatgg acagttactt
    20881 gagtgcttgc gtgaaaaaag aagtaccaga agcattggaa acattgaacc aaattctcgc
    20941 agccggcaaa gaatcacgtc gctttctaga ggatatgtta caatactgcc gtgacttgct
    21001 gatgtatcag caagcaccta acctagttga ggaacaatcg agtacgatca gaagtacatt
    21061 tacagaattg gcacaaactg tttcagcgga tcagctgttt tcaatgataa agattttgag
    21121 cgatactcaa aatgaaattc gttttaccaa tagtgccgct atttatttgg aagtcgctac
    21181 agtcaaactt gcacagctac cgtctgcttc tgttccttca gcagatgtag catttgcaga
    21241 tgatcagata gtaaaacagc tacaacaaga gattaaggaa ctaagaaacg aaatcaagga
    21301 actacgcagt aatacaggaa ttgagaatac aaaagtacag ccggtacagc aaacaaatca
    21361 taaaaaacag acaagtacct atcgtgtacc gacggaacga gtatataaag ttctgcggga
    21421 agcgacaaga cagcatctga accaagtaaa agaagtatgg gaagacttgc tgatgagtct
    21481 atctgtcact cagcgagcaa tgcttaaagc aagcgaacca gttgcagctg gacctacagg
    21541 actagtgatt gcgtttgatt atgagattgt ttgtcaaaga gcagcaaatg acgaagaatt
    21601 gcagctttcg gtacacaaca acttaagccg aatgatcaaa gattatgcgc ctgatcctgt
    21661 ttatattaca agagaaagct ggccagaact tcgaagaaat tatttgacgc aggcacaaga
    21721 agagggaaca gaaaactctt ttgcttctga agactcagaa ggagaaatcg agttgcttcc
    21781 tcaagaacaa gaagtaccag aagaaacact tgtagtaaaa gcagaggaac tgtttggctc
    21841 cgaagctgtt acagtggtag atgattaata aaaaggagta gataattatg atgcgtggaa
    21901 tgggcaatat gcaaggcatg atgaaacaag tacaaaaaat gcaaaaagaa atggcagaag
    21961 cacaagagct gctaaataaa acagagttta caggtaaagc caccaatgaa ttggtcaaag
    22021 cggtttttac aggcgatcgt cgcatgaaag atatccaaat caatgaagaa gtcgttgatc
    22081 ctgatgatat cgagatgctt caggatcttg tcgtaatggc agtcaatgat gcgatcgcac
    22141 aaattgatgt ggaaactgag aaaaaattag ggaaatacgc aaaaggtttg ccaggatttt
    22201 agttttaaac tgaaggagta atgaatatgc aatatccaga accaattgcg aaactgatcg
    22261 acagctacat gaaacttcca ggaatcggtc agaaaacagc gacaagactc gccttttata
    22321 cgatcgatat gaaagatgaa gtagtaaatg aatttgctaa atctttgtta agtgtaaaac
    22381 gcgatttgca tttttgtagt atctgcggaa acatcacaga agaagatccc tgcgaaatat
    22441 gtagaaatcc ttcacgtgat cgaagcgtta tcctagtggt cgaagagccg aaagatgtaa
    22501 tggcaatgga aaaaatgcga gaatatcacg gactttatca tgttctccat ggtgtgttat
    22561 caccgatgga agggacaggt ccagaagaca tcaatatttc ttctctgttg cagcggctgc
    22621 atgatgcaga tgtaaaagaa gtgattatcg caacaaatgc aacaacagag ggagaagcaa
    22681 cagctatgta tctctcacgt ctgatcaaac ccgcaggtat caaagtgact cgtctagcac
    22741 atggtctgtc tgtaggctct gatatcgagt atgcagatga agttacttta ttgaaagctg
    22801 tcgaaggaag acgtgagtta taaatttcta attaagtgtt ttttcagata caatataaaa
    22861 gcagcacaag agatgggggc aacaatgtga acggactatt tattacaatc gaggggcctg
    22921 atggtgcagg aaaaaccagc atcttgaatg aactttaccc tcttttgaaa gaagtagcaa
    22981 aaacagaaat cattcaaaca agagaaccag gaggcattcc gattgctgaa gagatccgag
    23041 cagtgatcct tgatccaaaa aatgatcgga tggatgaacg tacggaagca ctgctttatg
    23101 cagcagcaag aagacaacat ctagtcgaaa aggttctgcc tgctctggca aaagggaaaa
    23161 tcgttttatg cgatagattt gtcgacagtt ctcttgctta tcaaggtgca ggccgacgca
    23221 tcggcgttac cgagattgct cggttgaatg agtttgctac agaaggtact acgccggatt
    23281 ttacccttta tcttgacgta gattcagaca ccggattaag aagaatcaag aaaaacagac
    23341 aaaatcaaat cgaccgttta gattcggaag gtttggaatt ccaccagcgt gtacgtcatg
    23401 cttacttaaa gctggcagaa gagaatccgg aaagaatcca caaagttgat gcaagaaaaa
    23461 gctttgaaga agtgctgcaa acaagttatc atacaattat tgaacagtat ccgcaatttt
    23521 ttgaaaacta ggaaggtgaa cgtttatgaa aattatttta gcgattatcc aagacaaaga
    23581 cagcaaccgt ttatcaaatg aattgattga tgcaaatatc cgtgccacaa aactatcatc
    23641 gacaggcggc ttccttaagg ctggaaacag tacgtttatc gtgggtattg aagatgaccg
    23701 tgtagaagaa gcattggaaa tcatcaaaaa aacgtgtgaa tcaagaaagc aatttgtatc
    23761 tacacctgtt acactggata tttcgatgga tgggggcgtc ccttatccag tagaagtaga
    23821 agtaggcggt gccacagtct tcgtcttacc tgttgaagga ttccatcaat tttaggagga
    23881 gctgagctat gaatcaagaa ataaacttgg ctcaaatgca accgatcgtg tatcagcagc
    23941 ttcaacggag ttttgagcat gggcgtatcg ctcatgctta tctttttgaa ggggagaaag
    24001 gaacaggaaa acatgaagta ggtatatggc ttgcacagca cctgttctgt acccaaatga
    24061 aagacaatct gccttgtgga aaatgcaaca attgtcagcg gatcgaaaag aaagaacatc
    24121 cagatgtttt ggttattgaa ccggaaggac aaaccatcaa agtcgatcag atcagacgtt
    24181 tgcagacgga attcagtcgc agcggttacg aatcaagaaa aaaagttttt ttgatcaaag
    24241 aagctgaaaa aatgaatagc agtgcggcaa acagtttgtt gaaattcttg gaagagcccc
    24301 ctggagattt tttagcggtg ttagaaacag atgcagtagg aagaattctc cctactattc
    24361 aatctcgttg tcaagttctg cattttcaag aactttcgaa agaagcactg atccatcgat
    24421 tgcaagaaga gcagattcca ttggaaaaag caaaactttt ggctttctta acaaacagtt
    24481 taggaaaagc agttgaaatc tctcaagatg aatggtttaa tgatgctaag gacttgattt
    24541 atcaatggtt cgtttacttg caaaagcaag atactcaagc ctttatctat gtccaaaaaa
    24601 aattggtaaa atcatttaaa gaaaaaacac aacagtttac aggattatct gtcttgatgt
    24661 tttattatca ggaagcacag cgtcaagcag tagcaaacgg cgaatatggc aaagtgaaac
    24721 agataaacca gataattgaa cggatattga tcgcagagca gaaactacgc agcaatgtca
    24781 gttttcaggc tgttgcggaa cagttcgttt tgcatacgat tttttagctt gccgcaaagg
    24841 atgatgatca aggaggatgg aaatggtaga agtagtaggt gtccgatata gagatgcagg
    24901 tcatatctat tattttgcac caggagactc tgaatatgtt tatagtgaca aggtattagt
    24961 cgaatctcag caatccttac atattgctac tgttgtcctt cctaaaaaag agattgatcc
    25021 caatgacctt cctgaagcta tccgtccgat cttgcataaa gcgtcacaag ctgaattaga
    25081 aaaagacaaa aaaaacaaaa aagatgctta tgatgcattg aaaatcgcaa aagaaaaagt
    25141 gcagttgcgt gagttggaaa tgaaaatggt caaagccgaa tacacatttg atcgaaataa
    25201 attgattttt tctttcacag cggacgggcg aattgacttt agagaattgg taaaagatct
    25261 ggccgctgta tttcgtactc gtattgagct tcgacaaatt ggtgtccgcg atgaagcaaa
    25321 gataataggc ggaattggtc catgtgggcg tccattatgc tgttcttctt ttttaggcga
    25381 tttcgtgcct gtatcaatca aaatggcaaa agatcaaagt ttatctttga atccaacaaa
    25441 aatttccggt ttatgcggac gcttgatgtg ctgtctgcaa tatgagagcg aagaatacga
    25501 gacagcgaaa aaagaactcc ctgactttgg aaaagtaatt gctacacctg atggtaaagg
    25561 gaaagtcgta gggttgaatc tgcttagccg tatcgttagt attcggctga ctggacggga
    25621 aaatcctgtc gaatatgaat gggatgaatt gaaagaattt atccaagtag gtgagatgaa
    25681 tggataagag atcattatac gacggattga atcaattaga atcagagttg agaaatacat
    25741 taacagaact tgttgaaatg aagcatgctt tgcatgagat cgtagaaaaa aatacaactt
    25801 tggagatgga aaatcaacgt ctgcgggaac acctgcggga attgaaccaa gttgcacaag
    25861 ctccagctga aaatgtgaag caggaacttt ctaagtctcg tatgaatctt gaaaaaattt
    25921 atgaagaagg attccatgtc tgctacgatt tatatggatc aagaagggac aatgacgagc
    25981 cttgtgcgtt ttgtctagaa gtgatctatc gcgaaagcgg tcgatgacca ataacaagag
    26041 gagactggag cggagaaata ctgctccagt ctttttatta aggagattgt gacagatgta
    26101 taatcaaaaa agtttcaagg gaaatccggt atatggggat ttatatcttg taccgacgcc
    26161 aattggaaat ttagaagata tgagctacag aagtgttcgt atgctgcaag aatccgatct
    26221 tatcgcaagt gaggatacgc gtaatacaca aaagttattg aatcattttg aaatccatac
    26281 accccaaaaa agtttacatg aacataatta taaagaacgt gtccctcaat tgattgcttt
    26341 gttaaaagaa ggaaaaacga ttgctcaagt cagtgacgca ggaatgcctt ccatcagcga
    26401 tcctggacat gagctagttt tagcctgcat aaaagaaggg atttctgtga tctctatccc
    26461 aggacctaca gctgggatga cggctttgat tgcttcaggg ctagtgcctc aaccattttt
    26521 attttatggc ttccttccac gtaagaaaaa agaacagaaa gaaacattga aaaagctaaa
    26581 agaacagaca gttacgctga ttttttatga gtcaccttat cggatcactg ctacattgac
    26641 gaatttctta gaaatttttg gaaatcgcca agtcgttttg tgtcgggaat tgacaaaggt
    26701 ccatgaagaa taccttagag gaagtacgga agagctactc gcttatatcg aagaacatcc
    26761 agtaaagggt gaatgttgtc tgcttgtaga agggaatttg ttcgaaatgc cagtgacgga
    26821 agttgatgaa gagttgggaa cgttaaagga acaagtagaa gcaaagatcg cttcaggcga
    26881 aaaaccaaat gctgctatca aagcagtagc gctgaaaaat gggctgaaaa aacaagaagt
    26941 ctaccgtcag taccatgaat tagactgaga tagtataagt tgtctataat gaaagactaa
    27001 tgaggaaatc agctactaac ttttcaaatc tcttgaacgc gaacgaatgt tcttgtataa
    27061 tgaataagag gtgaggatga tggagctgca atttccttta cataatgata catcaagaaa
    27121 aatcattcat attgatatgg atgctttttt cgcttcggtt gaagaacgag atcatcctga
    27181 gtttcgcgga catccattag taattgccag acaccccagt gacactggtg ggcgtggagt
    27241 agtgactaca gcaaattatg aagcaagaaa attcggaatt cattcagcaa tgagtgcaca
    27301 aaaagcgtat gagctttgtc cgcaagccat ctttaagcct ggcgaccatc ataaatacag
    27361 cgatatttcc aaacaagtac gggaagtatt ctatcgttat acagatattg tggaaccagt
    27421 tagtatcgat gaagcgtatc ttgatgtgac cgaaaataaa atcaattgca agtcagccat
    27481 caaaatcgcc cgaatgatcc aagcagatat ttgggagagt gtccagctaa catgttctgc
    27541 gggagtaagt tacaataagt ttttggccaa attagcatca gactatcaaa aacctagagg
    27601 aatcacggta gttgcccctc aagacgctgt gccattttta aaagctttgc caattgaaaa
    27661 atttcatggg gtagggaaga aaactgtacc tagaatgcat gagctaggga tttatacagg
    27721 aaaagaccta tatgaatgca cagaaatgat gttgattcga aattttggaa aaatgggcta
    27781 ttctctttat cgtaaagtca gaggaatcca tgattctcca gtaaacgtta caagagagcg
    27841 aaaatctgtg ggaaaagagc atacttatgg gcagccgttg caaacggaag aagcagtgct
    27901 gactcagttg cgccaactag ctgaaaaagt agaagaagca ctgagaagag tccaaaaaca
    27961 tgggaaaaca gtagttttga aggtcaggta cacggactat tctactgtga ccaaaagagt
    28021 gacattgcca gaatatatct ataaaaaaga agcactcttt tatcaagcaa gccttatatg
    28081 ggaggaaatc ttgggagtag aaaaaggaat ccgattgtta gggattactt tgacgaatct
    28141 ggacccgatg acttatgaaa acatcgtcct tccattatga gaaaatcaag aaatataaaa
    28201 acagctttga gagttgaaac cggaaatccg ttttctctcc caaagctgtt tttatatcaa
    28261 gtcttgcttt ttgatttgtt cataggtttc tgttgcatcc gtacattttt cccaaatcac
    28321 aacttgattg gattctaaag atttttggac gtcaatgatc cgctggttgc tacttccgcg
    28381 aaattgtagt tttaggtcac gtttgcttaa ttcaaagcgt ccgtcaacaa ggatatcgat
    28441 ttgcgaaagg agttctaatt tatcttctga atcttgtagc aattcatcaa aggtatagcc
    28501 tgtccatgac cagatatctt ttgtcgtacc gaactctttt cggatacgtc gtacgacttg
    28561 cagacaaaca tctgtattca aaaaaggttc gccaccaagt aaagttaatc cttgcacgta
    28621 atcgttgcgc aagtcagcga tgatttgttc ttctaattca ggtgtgaatg gtttgccata
    28681 tcggaaattt tgtgcagctt tgttgaagca gccttcgcag gcaaataagc agccactaac
    28741 gtaaagactg ttgcggacac cttcaccatc gacaaaatta aacggtttgt agtcagcaat
    28801 atagttttgg ctgtagtctt ttgctaacca ttcttttggt tttggatttc tcataaaaaa
    28861 acttctttca tttaaggttc aaagatatag ctattgaaga taaaatctac tcgaccacat
    28921 aaataagaac aaactgacct aaatccattg ttcaagaaag cttgttctta ttttgataga
    28981 gggcgttcaa agatacaaag ccatcgaaag agggtggtga aaaaagactt gtcatagcct
    29041 cttattctga ataaacggtg ttcaaaagct tgcatctgca gtaataagcc caactaaacg
    29101 aaaaatatga ggaactattt ttgtttagct attcttatta cttgatgcag gacagctttt
    29161 tcacaacctc atttcatgtg tttcacacgt gaggaaattt ctttatggcg gccatgtacc
    29221 atcggacgag cttgcggatt tcctaaatag ccacaagtac gtttgactac atcacatgtt
    29281 tttggatcat gatttccaca ttctggacat tcgaaccctc gttctgtcgg tttgaaatca
    29341 ccttcgaaac cacattgata gcaatgatcg attggcgtat tcgtaccaag atacccgatt
    29401 ttgtcatatg caaaatccca tacagcttct aaagctttcg gattttgttg gagcataggg
    29461 tattcacagt agtggatgaa tccacctgaa caatattttg gataatcctt ctcaaattcc
    29521 aatttttcaa atggcgttgg gtttttacgc acatcatagt ggaaactgtt tgtataatat
    29581 tctttatctg tgatattttc gatgatgccg aatttttctg tatccaaacg gcagaaacgg
    29641 tcagttagac tttcacttgg agttgaatag acactgaaat gataaccata ttcatccccc
    29701 catgcatctg cattagcttt taattctttg acgatatcaa gtgtgaaagc ttttgcttct
    29761 gggtttgttt cccattcgcc accgaagaaa gctgaagcaa cttcatacaa tccaatgtag
    29821 cctaacgata cagtagctcg acggtttttg aataattcat tgacagaatc ggtacgtgaa
    29881 agacgtttcc cgaaagcacc atacatatat aaaattggcg catttgctgg aatggcttct
    29941 ttacaacgtt ctacacgata gagaagcgca tctttcatga tttctaagcg gtcagacagt
    30001 aactgccaga atttattctg atctcctttt gattcgatcg cgattcttgg caaattcaat
    30061 gtaacaacac ctaaattcat tcgtccgaca tttacttctt gaccattttc gtctttccac
    30121 ccttgcaaga atgaacgaca gcccataggt actttgaaac ttccagttaa ttcaacgatt
    30181 ttatcgtaat tcagaatatc aggatacatt cttttcgttg cacattccaa tgctagttgt
    30241 ttgatgtcgt aattaggatc ttcagggttt aagttcacac cttttttcaa agtgaagatc
    30301 agtttaggga agatagctgt ccgcttttca ctccctaatc cattgattcg aatctgtaaa
    30361 attgcttttt gaatttctct ttcaaaccag ttttctccta gtccaaatcc taatgaagtg
    30421 aaaggagttt gtccatttga agtaaataag gtattgattt catattccaa actttgcata
    30481 gcatcgaaga tatctttttt ggtttttgct tttgcatatt ctttttggcg ttctggcgaa
    30541 tcgatccatt cttccgcgtc ttttagatgt tttttatagt tcaattctgc aaaaggagca
    30601 agcaattcat ctgttcggtc ggcagaacag ccaccatatt gacttgacgc tacattggca
    30661 ataatctgtg agatttgtgc tgtggcggtc tggatagatt ttggcgattc gacttcggca
    30721 ttgccgattt tgaacccatt gttcagcatg cctttgaaat caatcaagca gcaattcgtc
    30781 atcggtgtat aaggatgata atccaaatca tggtaatgga tatcgccttt ttgatgcgca
    30841 tttgctatat gtggtggcaa cattttcagt ccgattgatt ttccaacgat cccggctgtc
    30901 agatctcgct gtgtattgaa aacattactg tctttatttg cgttttcgtt cacgacagtt
    30961 tggtccttgt tgattagctt ttcaatcgaa aagttgatat ctgttgcttt actgcgagca
    31021 aaatctcgtt tggtacgata attgatgtac tcttctgcta attcatattc gtttttttct
    31081 agtaatatat gttccacgat gttttgaatc tcatagattt tgacattttc agcaaaccgg
    31141 cttttgattt cttggacaac caagtctgca atcatttgaa ttttttcata agtaagtggt
    31201 gataatgagc cgtgaatttt cttttctgct ttgatcagtg cttcattgat cttttgttca
    31261 tcgaaatcaa catggcggcc atcgcgtttg atgactttca tctgggatgt ctctggctgc
    31321 tgagtaacga cagttttttc ttcataattt actttcatta tcttcaactc ctgctaaatt
    31381 gttcttgtct atcataaaac aaaatcgggg aaggagcaac taaatatcgg ctatatatag
    31441 tattcaatgt gctgtataat tggatttcta tactatatgt agtgtatatt ttattgtgaa
    31501 aaaaggtgaa aacttgtagc tggtggattt gttttttgca tatttaattt tgctgaataa
    31561 aataattgaa taaaattttt tattcagtcc aagtaaaagt gacaaaaaca taagagcaaa
    31621 ttattcgtag atatgctact atgcaaataa atagaaatga aaaatgaaag aggaggagaa
    31681 gaattggaaa agatgatcga agtgaaacat ctaagtaaaa gctacggcag caaactttct
    31741 cccgtcaaag ccttagataa tatctcattc acagtagaag aaggcgagtt tgttgggatt
    31801 atgggcccaa gtggtgcagg aaagacaacg ctgatgaata tcttgtccac gatcaatttg
    31861 cctacgatgg gcagtgtctc gatccaagga aaagaaatca caaaaatgaa aaacagtgag
    31921 ctcagtgact ttcgtcgaaa aaaactagga ttcatttttc aagagttcaa tttgatcgat
    31981 accctaaatg caaaagacaa tatcctattg cccctagcag ttgaacggat gactaaagag
    32041 gaaatggaaa agagagtaag acatgtagct caattgttga atatagaaga actgctcaaa
    32101 cggtatcccg atgaactttc tgtcggacag agacaacgaa ttgcagctgc tagagcactt
    32161 gttgttcagc cacaagtgat ttttgcagat gaaccaactg gctctttgga ctcgaagtcg
    32221 gctacagaat tgttgaacta cttgaagacc atgaatcaaa aagaaaaagc aacgatactg
    32281 ttagtcaccc atgatccata tacagcaagc tactgcgata gaattttatt tattaaagat
    32341 ggtgtgattt tttctgaagt tgtcagaaga gggacaagaa gagagttctt tgagaaagta
    32401 atagacatgc aggcgacgat tggcggagga ggaaaaatga atgctgtata aactagccgt
    32461 taaaagtttt tttcggcagt cacgaggcta tcttgtttat tttttcagtt tgactttatc
    32521 cgtaatgatc tattattcct tcagcgcaat gacctacgat caatctttag tcagaagagc
    32581 aagtcaagat acttcgatag atggtggatt gagttttggt ggatttatca ttactgttgt
    32641 tttgcttttt ttcgtattca gtgccaaccg atttttcttg aatagaaggc aaaaggaaat
    32701 aggcatctat cagctttttg gtatgaataa actgcagata tcaggtatat atgtgttaga
    32761 aatcatgata attggattgt tcgcctgtat ttctgggata ttactcggta ttattttttc
    32821 caaactcttt tcaatgattt tggttcgaat gatggatatg gatcttacta gtccattttt
    32881 tatctccatt ccatcagtag tagatacttt atttgctttt ttcatcatcc ttttagcagt
    32941 atctgtatat tcaatatgga aaatatggcg ctatccaatg attcgaagtt ttggagaaaa
    33001 agaacaagca gatagttcaa caatgcgctt caggacaagg caccgattac tcgcagtcat
    33061 cgggttacta cttttaacga gcggatattt tggagctagt cattttaggg aaatcacttc
    33121 atggatgatc agttatcatt atcttgatac attagttttc ttttttcctt ttttaatttt
    33181 tttcgtttgt gtggtcggta cttatttgtt tttctgttat acatttcggt tatttttgat
    33241 catattgagt aaatggaaga tttattacta tggtatcaat tttttgataa tgggtaacac
    33301 gcaagttcat ttgctcaaaa gttggcgtac aaattctctg attacagtca ttctcggaat
    33361 ttctttaatg acaattggcg gcacggctag tatctctact attttatcct accaggagaa
    33421 agtttcctcc ccgatggact atcagtttga tgttgagaca gagaaaaaaa tcaagccgat
    33481 tctagcagag gaagggcaga agataactaa agaaatcacc ttgcattata aagtcattgg
    33541 gagtatctat agtcaaaatc tcgaagaacc tatgtttcaa actgctaatc tgatcacgga
    33601 aacacaatat caagccttca gaaaagttaa tccagatttg ccgaacattt ctttgaaatc
    33661 acctggtcat actgttttgc ttgatcaaat gcaaactctt tttcggaata tttcattata
    33721 tggatctagt attggactgc cagataatca agaattgaag attcaacaaa tgcttccaag
    33781 tgtattaggt gacgaaacaa tggtatatct aagtcctgta ctgattgtag atgataccgt
    33841 ttttgaaaaa gcaaaaggtg tggattatca agtagtcaat gtagatgtat caggtggaaa
    33901 taatgaaaaa atagatgaac gactgagcaa tgacctgtcg ataaagtggc tcaatgctgt
    33961 ttattattct tatgatatcg taaatggaag tctacaagga gaaattagca caaagaaact
    34021 tccggatgac caaatgaagg aagcacaatt tagccaagaa agctctcgtt tgaatttctc
    34081 ttccagctat tcgaaattgc gtgcagttaa tcggcgaatc ggtatcaata tttttgtcac
    34141 actatttgtc gggatgatcg tgattattgc tactggtagt attttgacag tccggcaatt
    34201 agcagaagca gaggaagaaa aagaaaatta tcacctattg actaagctgg ggattccaca
    34261 aagaaggatc agacggatga tcttcgagca aaacgcactt accttctttc cacccatgat
    34321 attagggatc actcacgcag tatttgcaat caatgtcttc actcagtata taaaaactgc
    34381 cgattactgg ttggcttatc ttgtgagtgg cttattgatc cttatctatc ttttgcttta
    34441 tgtcattacg agtcagttat attgtcggat tatcgaggaa tgatatacgg aataagacaa
    34501 gataataaat agaactaaat tttgaatacc atttatttgg aataagaggt tgtgacacga
    34561 tttttgtcac aacctcttta ttttttatat tttttaaaag tacgagaaat tataaagaaa
    34621 aaagcagtga tttgatgtgc tatttttata aaagctatgt gacaaaactg ttagtaaaaa
    34681 aacggtttct gtaagctaga cacatgtttc tttttgtcaa aattagttac actttgtgtg
    34741 aggttatatg aggagggaat ccaatgaaca aatggcgttc taatccaaaa ttgaattatt
    34801 ggggaaaatt catcattcga acaatttttt atagtgcagt actgctgttc ctgatttatc
    34861 tgtatcacta taaaaatatt caaggaggaa cttttatcta taacgaattt taggaggaag
    34921 agtatggaaa tcaaaacgat tattgaagca atcgatgatt ggggaataaa tgaacctgat
    34981 cgagttgctt atcaggcgga tgagacgcat acttttggtg aattgaaaca agcatcagac
    35041 gcacttgctt attatttaga aaaaaaggta gaaggcgatg gaccaattgt cgtttttggc
    35101 aatttggagt ttgaaatgat tgtctcattt ctaggggttg tcaaatctgg ccatgcctat
    35161 ttgccgattg aagagcatac acccaaggag cgtatcctat cgatctttcg ggtggctaaa
    35221 ccatctatgg tcatcagtat cggggactgg atagaagata ttccttcggt tcctgtcata
    35281 acaaaaaaag aatttcaaga aatcaagaag ataccagtag gattttatcc cagaaatagc
    35341 gtaaaaggta cagaaaattt ttatattatt tttacttctg gtactactgg agaaccaaaa
    35401 ggcgtacaaa tcagtcatga taatctagtc agctttgttc aatggacgat ggaagacttt
    35461 gggatcaagc ctggtatgca tttcttagcc caagcgccat tttcttttga tttatcggta
    35521 ttttctattt atcctgcatt agtttctggc ggaatgctga agccgttaaa taaagcagtt
    35581 gttcaagact ttcgtcaatt attcgcgaca ttgccagcct tgaagttgaa tgtgtgggtt
    35641 tctactccat catttatgga tatttgcttg atggaaccta cattcaatgg agaaaatgtg
    35701 ccggaattgg acatgttttt attttgcgga gaagaattga cgaaaaaaac tgcagaaagt
    35761 ctacttgatc gattcccaaa tgcacggata tataatactt acggtccaac agaagcaact
    35821 gtagctattt caagtattca gattgaccag catgtactag atagttatga ccgactaccg
    35881 attggctacg taaaaaaaga cacgaaagtt tctattgtcc aagaaggtaa acctgtttca
    35941 aaaggagaaa cgggtgaaat cattattgca ggaccaagcg tatcaaaagg ttacctgaac
    36001 aatcccgaaa agacagctgc tgcgttcttt tgtcaaaatg acttagcaag ttaccataca
    36061 ggggatgcag gtagactaga tgaatatggg cttttatttt atgaaggacg tatggatcag
    36121 caggtcaaat tacatggata ccggattgaa ctcggggata tcgaacatta tctgctccaa
    36181 gacaaccgaa tcaaacaagc tgtcgtcgta ccaaaatacc aaggaacaaa agtacagcag
    36241 ctggttgctt ttgtcgtatt agaaaacaaa acgaaacagc cggattttca gttgactaaa
    36301 tctatcaaag aacaattact agaagttgta atggattata tgattcctca gaagttccat
    36361 tatgtcgatc tgctgcctca gacagttaat ggaaaaatcg atcgaaaaaa attaacagct
    36421 gaggtgaatc ctgaatgatg ttacctcata tgatccctta tgcagagccg ttttattttt
    36481 ttcttttagc cattgcgctt gtaccgatcg ttctatcttt attgatatgg gaaaaaagat
    36541 taccagttta tcaatcagca gtgacacttt tctttttatt tgtcagcttt ggaggtaatt
    36601 attggcaaca gggagcggcg ctgattgggt atgttttgtg gcagacgata cttgtatggg
    36661 gctattttgt ttatcggcaa aggaaaaatc agacaggatg gttttttgcc gccgttattt
    36721 tagcgataat tccactgttt ttaacgaaag tgactccttt ttttgaacat ggaaaagcat
    36781 cgttacttgg ttttttagga atctcttatt taacctttaa atctgtacaa atgatcatgg
    36841 aaataagaga tggcatgatc aaaacatata agccatttcg ttatatccag tttctgctgt
    36901 tctttccaac aatctcttct ggaccaatcg atcgctatag gagatttgaa aaagatcagc
    36961 tccatccgcc tgttcgtgat aaatatgttg atcttttggg aaaaggcgtc caaaatattt
    37021 ttgtcggttt tttatataaa ttcattatcg gttattattt tggtcaagta ctgcttcctg
    37081 tagtgggacg cattgcctta cagcatggtg gtatttcatg ggcattagta ggttatatgt
    37141 acgtgtacag tatgtatttg ttttttgatt ttgctggcta tagcttattt gcagtcggta
    37201 caagctacat gatgggatat cagacaccaa tcaatttcaa taaacctttt ttatcatgga
    37261 atatcaaaga attctggaat cgttggcata tgacgctgtc cttttggttc cgtgattatg
    37321 tatatatgcg attgatgttc ttcatgatga agaaaaaatt ttttaaaagc aagatcgtaa
    37381 tgtcaaatat cggttatttt gctttgtttc ttctaatggg tatatggcat ggattgacgt
    37441 ggtactacat cgtatatgga ctgtatcatg cttgtctgat ctgtctgaca gatgcatgga
    37501 tccgatttaa gaagaagcat aaagagcagt tgccttctaa caaatttacg catggcttag
    37561 ccgtattttt gacatttcaa gcagtatgtt ttagcttcct gattttttca ggatttttag
    37621 ataaactttt ttttagctaa cgaatagtga catattaatt tatataacaa aggagaaaag
    37681 gaaaatggaa gaacaaattt tatcaatctt agaagaaata acaggaacag atgaagtaag
    37741 aaaaaatcgt gatattgatc tatttgatga agggttgctt gattctttag catctgttca
    37801 attattagtc gagctagacg gagagctgga tatccaagtc cctgtatctg aattcgagcg
    37861 agaggattgg agcacaccga ataaaatcat tcaacaagca actgcattga agggttgagt
    37921 atgaaaaaga aactttttgc tattttcggc ccggttgttt tagctgttgg cttactgttt
    37981 gtgtttttta cctcctccta tcaagtagat atctctaatc cagaaacgat tagacgtgcc
    38041 tcttcttcca tgtcagtgaa cgtgttgaaa ggagatgcga taaaaaatta tgcattatct
    38101 gaaaaacaat atattccttt ttttggctca agtgaattaa gcagaatcag tccatttcat
    38161 ccgtctgttc tggcagaaaa ataccagcga aactatcgtc catttttgct gggagcaccc
    38221 gggacacaat cgttaagcca atatatgatg atgcgttctg caggagatgc tatgaaaaac
    38281 aagaaagtcg tctttattat ttctcctcag tggttcgtta aaaatggtgt aaaaactgat
    38341 tactttaata cgtattattc tgaacttcaa acatacgact ggttgttttc aatgaagaaa
    38401 gtaacgcctg ctgatcgcta tttagctaga agattattaa ctttctcaaa agtaaaagag
    38461 aatgatacgt tgacggcgat tctgcaaacg atcaaaaaag ggaagctacc tttacctgaa
    38521 tctttgaatc agctgcgcag ccaatggaac atgctgaaga gagaggatga agtattcagc
    38581 aatattgggt tgaaagatcg acagcaaaaa atcgatcatg agtcaaaacg attaccaaaa
    38641 caataccaag aaacagaatt atccatttta gcaaatcaaa ttggagaacg agaaacgacg
    38701 aataatccat tcgggttgaa aaacgatttt tatacgcatc gtatccgagc ccacgagcca
    38761 gagctcaaac agtcacaaaa aaattgggat tatcgttttt cgccagaatt ttctgatttt
    38821 cagctggtct tggatcaatt agcaaaaaac cataacgaag tattgttcat catcccaccg
    38881 gttaacgaga aatggagcga ttataccgga ctatctcaag aaatgttaca aggatttgcc
    38941 aaaaagatca aatttcaatt aaatagccaa ggattcaatc gaatagctga ttttgtcaat
    39001 caagcgggta ccaactattt tatggaagac acgatccatc taggctggaa aggctggttg
    39061 gctgcagatc aacagatcag accgttttta gaagaaaatc atattactgc tagtaagtat
    39121 catcttgacg atgctttttt tagcaaatct tggcaacatc aaataccaga taaactacaa
    39181 ttaaaataaa gaaaacaggc gagtgttgct cgcttgtttt ctttattttt gaatattctg
    39241 aataaaagat aaaaatacca agtttgcctg aacaaaaatc ataagcaatg gtataatcac
    39301 aatatgaata tagaaatggg ggaaaacatt tggatacaaa agaagaacag ctacttattg
    39361 atttaacttc tgcaaaagga gtacctggaa atgaagaaga ggtaagagaa gtattcagag
    39421 aatatgccaa accattcgct gatgatattt tttatgacgg tttaggcagt gtgattgcca
    39481 aacatggtgc tggcggaggc cccaaggtct ttatttctgg acatatggac gaagttggtt
    39541 ttatggtaac aaaaataacc gaaaaaggat tcttggaatt ccaaactctt ggaggttggt
    39601 gggggcaagt catgcttgct cagcaagttg aaatcaaaac acgtgaaggg aaaatcgtcc
    39661 acggtgtgat tggttccaaa ccaccacatg ttttaacacc tcaagtaaga aataaacctt
    39721 atgaaataaa ggatatgttc attgatattg gcgcatcaag ccaagaagaa gccaaagagt
    39781 ggggcatacg tcctggagat atggtcactc catatattga atacaaacga atgaatggca
    39841 gtaaatattt actggcaaaa gcatgggata atcgtatcgg gacagctgtt tctctgcgag
    39901 tattagagaa tctatctaaa gaagcacacc caaatgtttt attcgcagga agcgatgtgc
    39961 aagaagaagt aggcttgcga ggtgctcgaa caagtacaca ccttgtaaat cccgatattg
    40021 cttttgcttt agatactggt acagcaggag acactccagg aatgacacca aaagaagcag
    40081 attcgatttt aggaaaaggc ccacagattt tgatttttga tgcatctatg atcccgcata
    40141 aaaaattatt gaattttgtt attgatgtag cagaggaaat ggatattcca ttccaatata
    40201 cagtgatttc tggcggagga actgatgcag gacaaatgca tttgacaaga gatggtgttc
    40261 cgtctgtagc cattaccgtt cctgttcgtt atctgcattc ccatacatca gtgattcatg
    40321 aagatgatta tttgaataca gtgaaactag tcacagaatt agtgaaacgg cttgatagtg
    40381 aaacagttga aaaaattcgg agttatgaat aatgaataat agaataccta tttctgaata
    40441 tcggaaaaaa gcacgagttt ctttggaaaa tcaatgggga atcaatactt ggcttgtttt
    40501 cttatctata atatttacga gcgtcatcca gaactttttt gacggtttca gtccttttcc
    40561 ggaagaaagc atgcagcagc acttattgag ttttctagtc gataatttac ttttattcgc
    40621 attcaactat gcattgtttt acattgcgct agtagttgta cggggtggca gaccaaaaag
    40681 taacctttta ttttcagttt tccaaagaaa gtattttggt tcgatacttg gcatcaattt
    40741 gatcaattca gtcatcaact ggctactcaa cagtttaatc tttctgccca tgtttttaat
    40801 cagcggagtg agtacttata tgcagctgct tttcagttca aatgcggtac ctacgggtgt
    40861 catgccagaa gattttcttg atatcagctt tgttttatcg atgataggga tgatttttgt
    40921 ttcagcactc atcatgagta ttgttggcgg tttattccaa tttgctgcat ggacaaagtt
    40981 cgactatcct gatttgtcgg ttattcaatg tttgaaatat ggctggtttt tattgaaaga
    41041 tagaattggt acgtatattt tgttgcagct gtcttttatc ggctggtatt tgctaggagc
    41101 aatcgctttg ttcatcggtc ttttgtgggt cattgtttat gtgaatgtgt cagttgcaga
    41161 attttatgaa caagcacgga ttgaaaaagg aggtccagac gcctatttta cttagcatgt
    41221 aagcagttta ttcatcaaaa aaataacttt tgtggtagga taaatctttg agggggaatg
    41281 tacgatgaaa ttaactgttt taggatgctt aggagcgtat ccttataaaa atcaagggac
    41341 aacaggttat ttgcttcaat ctgacgggtt caatttgttg atcgatgcag gtagtgctac
    41401 gctgatcaaa ttacaagaac accttgatcc acttgatcta gatgctgtga tccttagcca
    41461 ttatcatcat gatcatattg ctgaccttgg tgtattacag tattactggc aacttcatcc
    41521 taagagaaat ccggagacat tgctttcaat ttatggacac acaaaagatt ccattcactt
    41581 tgaagaattg acgttggaaa atgtctcaaa aggacatcct tattttgaag ctgaagaatt
    41641 aaaaattggt ccttttctga ttacttttat ggaaacaatc catccagtag tatgttacgc
    41701 catgcgtatc gtagaagaaa caacggggaa agtattcgta tttacaggtg attcaggtta
    41761 tttggaaagt ttcgctgatt ttgcgaaaga tgctgatttg tttctagcag atacctatct
    41821 ttttgaagga aatgaacatc atcatgccca tttcacttca aaagaagctg gagaacttgc
    41881 gaaatcagct aacgtaaaga aacttgtact tacacaccta cctcagtttg gtgatttaga
    41941 acaattgaaa aatgaagcgc aaaaggcagc aggcaaaggg attgatatag aacttgctgc
    42001 agttgacaaa gtgtttaaca tttaggagga agaacatgat ttttgtacca aatgaaaacc
    42061 acgacccaag aatcaattta gcaattgaac aatatttgct gcaagaaatg cctttagatg
    42121 aacctatttt gctcttttat atcaatgaac cttcaatcat catcggcaga aaccaaaata
    42181 cgcttgaaga aatcaatcga gattatgtgg aagaaaatgg cattcatgtt gtccgtcgct
    42241 taagtggagg cggagctgtc taccatgatt ttggaaacct gaactttagt tttatcatgc
    42301 ctgatgatgg ggattcattc cgtgactttg cgaaagtaac acagccaatc atccaagcac
    42361 ttcatgaatt gggtgttgct ggggcggaat tgaaaggccg aaatgatcta gtcattgatg
    42421 gcatgaaatt ttctggcaac gcgatgtatg ctaccaacgg tcggatgttt gctcatggaa
    42481 caattatgtt cgactctgat atcaatgaag tagtcaatgc attaaaagta aaaaaagaca
    42541 aaatcgaatc aaaaggaatc aaatcgatcc gttctcgtgt aacgaatatc aaaccatttc
    42601 ttccagaaga aaagcaaaaa atgacaacag aagaatttcg tcaagaaatc ttgttgaaaa
    42661 ttttcggcgt agagtcagta gaagaagtaa aaacgtacga attgactgat gaagattgga
    42721 aaaaaatcaa tcgaatctct gaggaatatt atcgaaattg ggattggaat tacgggaagt
    42781 ctccagattt caatttctct cgccaaaaac gtttttctat cggttcgatt gaagtccatt
    42841 tgaatgtctc agaaggaatg atcaaagaag caaaaatctt tggcgatttc ttcggactag
    42901 gagatatctc tgacgtggaa aaaaaattgg ttggacagaa atatgataaa gcatctctat
    42961 caaaaatagt cagccaaatt gatgtgaaga aatattttgg tgcgatagaa ccagaagaat
    43021 tgcttacctt actttattga aaataaaaaa tagcggaatc cacatatatg gattccgcta
    43081 ttttttatac aggattcggg taatttcgag agagttgaac agtgatccga tcaatgaaag
    43141 tatctttttc ctgcgttagt gttcctgtgt aacgatccgc tacgtctttc atcatcatta
    43201 agtcaaaacg ttcctgatca tctaattctt ctgtgatgcc tcgactaaat aggtttgtat
    43261 cgtaatgtgc actatgttcg caacaaatga cgaaatgttc ctcttgttcg tgtatcgaca
    43321 aataaatttt tcgatacagt ggattttcga tggcatgagt ttctctaaag gaatgctcta
    43381 aagcgtggat caaaagttga gccagatcgt catcgttgat aggaagttct tcgggaagat
    43441 ctgcttggaa ggttatctga atattccgtt tggcagctaa cttctggtaa cgcgctaaga
    43501 taagatttgt cacctggtgg ttgcaaaaag aacacagcat ttcgaaattt tttgcttctt
    43561 ctaatatctt ttccacatag tttttagctt ttgtttggtc cgtatgcatt aaatcttgca
    43621 gattctccat ctcttggaca aattccaggc gaagtgtatt tacttcttgg atatgctgtc
    43681 gaagctgctc ataatgttgc tccaacaaat catttttgat ttttaagaaa tctacttgtc
    43741 tccgatcttt gtcggtattt aagatgtgat cgattagatt gtagctgata ataacgatta
    43801 gaatgataat atataaaatc gtagaccaat tgatcgttgt ttgatagata cctaatgccg
    43861 tttggatgta aatcaagcaa tggataatgg ctaccaagac gatccaaggt gtacaagcta
    43921 cataaaaacg gttattctta tatgcttcgc caatgcatgc aagtgaagtg actaaagtgc
    43981 ttataacact agcaatatta gcaatcggca ttaattcagg aagttctgca ggagtaaaag
    44041 ttgcataaag cagaacgccg ataaaaatca aaagttggaa cgtacaaaaa attccgtacc
    44101 attttttata gtaaaacatt cggctccaat agtagcagat gatgaaagct gacgttaaga
    44161 taataaataa atgagataaa aaagaatgaa cgaatggttc aaaaagaata ccacttaaaa
    44221 tatcttctga tacagcttca aatcctaatg ccaaagcaaa gcaacttaag agaataagtg
    44281 agtattcaag acgtttttgt ttatacatca agtagatcgt atagaaaaag gtccctatag
    44341 cgataaaaac agcgattatc atcgtcaata cttgcggcac tgattgtgaa taaataaaac
    44401 taatcaatgg tccagttgtt cctagataaa ctttcggtgg cagtccagca tagttctgat
    44461 aaggagatga gactgtaagg gtcaattttt ttccgatata atttgctggt aaatctatca
    44521 cagccaatga gagtccggga ttgctttctt cctgataatc tttccgttgg tataaaatct
    44581 tttcgcctag atatacagta atccattgat ggtttccttt gattagtaaa tttgcttcgt
    44641 ccaacggttt ttcaagtgtc gtttccataa tgaatgtttc gtctttatcc acggtaggaa
    44701 tcgtacgagt gtacttgctg tcaaacttaa aatcaggatc atcttttgta tagaaaaccc
    44761 acgaatcttt tatttctttc atttgattgc tgtccgcagg agaaaaaata aaataaaaga
    44821 aataagcaat tcctgttaaa aataacaatg ataatataat gattatgctc cactttatac
    44881 gtaaataaaa agattctttt ttattatata tgttattaaa aaaagacata aaggtattcc
//
LOCUS       DAZKKW010000015        44822 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.015, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000015 DAZKKW010000000
VERSION     DAZKKW010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 44822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44822
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.015"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..610
                     /locus_tag="ACG8PO_000791"
     CDS             <1..610
                     /locus_tag="ACG8PO_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFQ0206142.1"
                     /translation="KRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWIQLIL
                     DEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSKITVF
                     PKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAYTIQN
                     ATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMD"
     gene            723..1001
                     /locus_tag="ACG8PO_000792"
     CDS             723..1001
                     /locus_tag="ACG8PO_000792"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0206143.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            1119..1355
                     /gene="secG"
                     /locus_tag="ACG8PO_000793"
     CDS             1119..1355
                     /gene="secG"
                     /locus_tag="ACG8PO_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFQ0206144.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            1458..2213
                     /locus_tag="ACG8PO_000794"
     CDS             1458..2213
                     /locus_tag="ACG8PO_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFQ0206145.1"
                     /translation="MRRVRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKAD
                     YTVYAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFA
                     VRALEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSL
                     AEQQLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQW
                     YPESGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            2216..4573
                     /gene="rnr"
                     /locus_tag="ACG8PO_000795"
     CDS             2216..4573
                     /gene="rnr"
                     /locus_tag="ACG8PO_000795"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFQ0206146.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILIEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSEEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDITGRRDLRNQLIVTIDGEDA
                     KDLDDAVTVKKLDNGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHDIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILETMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKARNGKSGKRE
                     GKQAGKRSEKNGKKNKRYSDSKQTFGKKKGKKPFYKDVKKKKKKK"
     gene            4595..5059
                     /gene="smpB"
                     /locus_tag="ACG8PO_000796"
     CDS             4595..5059
                     /gene="smpB"
                     /locus_tag="ACG8PO_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFQ0206147.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIVRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            5361..6080
                     /gene="atpB"
                     /locus_tag="ACG8PO_000797"
     CDS             5361..6080
                     /gene="atpB"
                     /locus_tag="ACG8PO_000797"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFQ0206148.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMMTSLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            6133..6348
                     /gene="atpE"
                     /locus_tag="ACG8PO_000798"
     CDS             6133..6348
                     /gene="atpE"
                     /locus_tag="ACG8PO_000798"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HFQ0206149.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVIALILVFAV"
     gene            6439..6963
                     /gene="atpF"
                     /locus_tag="ACG8PO_000799"
     CDS             6439..6963
                     /gene="atpF"
                     /locus_tag="ACG8PO_000799"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFQ0206150.1"
                     /translation="MLNQLAIAEVGNAMLGNIIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            6950..7492
                     /locus_tag="ACG8PO_000800"
     CDS             6950..7492
                     /locus_tag="ACG8PO_000800"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFQ0206151.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYEYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMDYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLEKMRNQLNR"
     gene            7515..9071
                     /gene="atpA"
                     /locus_tag="ACG8PO_000801"
     CDS             7515..9071
                     /gene="atpA"
                     /locus_tag="ACG8PO_000801"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFQ0206152.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGNNSST
                     EDKLKSIQNA"
     gene            9085..9987
                     /locus_tag="ACG8PO_000802"
     CDS             9085..9987
                     /locus_tag="ACG8PO_000802"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFQ0206153.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMYQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            10010..11416
                     /gene="atpD"
                     /locus_tag="ACG8PO_000803"
     CDS             10010..11416
                     /gene="atpD"
                     /locus_tag="ACG8PO_000803"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFQ0206154.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            11430..11852
                     /locus_tag="ACG8PO_000804"
     CDS             11430..11852
                     /locus_tag="ACG8PO_000804"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFQ0206155.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
     gene            12321..12551
                     /locus_tag="ACG8PO_000805"
     CDS             12321..12551
                     /locus_tag="ACG8PO_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 family protein"
                     /protein_id="HFQ0206156.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            12648..13949
                     /gene="murA"
                     /locus_tag="ACG8PO_000806"
     CDS             12648..13949
                     /gene="murA"
                     /locus_tag="ACG8PO_000806"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFQ0206157.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            13962..14138
                     /locus_tag="ACG8PO_000807"
     CDS             13962..14138
                     /locus_tag="ACG8PO_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFQ0206158.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            14273..14569
                     /gene="yidD"
                     /locus_tag="ACG8PO_000808"
     CDS             14273..14569
                     /gene="yidD"
                     /locus_tag="ACG8PO_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFQ0206159.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFQLTRNKKDKKEPDYKKVVKNTSSTER"
     gene            14753..15219
                     /locus_tag="ACG8PO_000809"
                     /pseudo
     CDS             14753..15219
                     /locus_tag="ACG8PO_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            15304..16188
                     /locus_tag="ACG8PO_000810"
     CDS             15304..16188
                     /locus_tag="ACG8PO_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFQ0206160.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKAQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLN
                     GDKTFSKLGTAVQMAWRYCERKGLDKNSYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            16302..16538
                     /locus_tag="ACG8PO_000811"
     CDS             16302..16538
                     /locus_tag="ACG8PO_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HFQ0206161.1"
                     /translation="MMSKKNKDVEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYG
                     PKEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            16626..16706
                     /locus_tag="ACG8PO_000812"
     tRNA            16626..16706
                     /locus_tag="ACG8PO_000812"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:16660..16662,aa:Tyr,seq:gta)
     gene            16713..16784
                     /locus_tag="ACG8PO_000813"
     tRNA            16713..16784
                     /locus_tag="ACG8PO_000813"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:16745..16747,aa:Gln,seq:ttg)
     gene            16909..17079
                     /locus_tag="ACG8PO_000814"
     CDS             16909..17079
                     /locus_tag="ACG8PO_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0206162.1"
                     /translation="MKWKEKVDEAAEKLYDLVKSEKYNIEVNIPKKGGKAVQVKSKRP
                     TNHTKKWMAKNR"
     gene            17299..18006
                     /locus_tag="ACG8PO_000815"
     CDS             17299..18006
                     /locus_tag="ACG8PO_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0206163.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(18066..18710)
                     /locus_tag="ACG8PO_000816"
     CDS             complement(18066..18710)
                     /locus_tag="ACG8PO_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HFQ0206164.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGPVSYHEQLVTDHI"
     gene            18837..19124
                     /locus_tag="ACG8PO_000817"
     CDS             18837..19124
                     /locus_tag="ACG8PO_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0206165.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            19232..19552
                     /locus_tag="ACG8PO_000818"
     CDS             19232..19552
                     /locus_tag="ACG8PO_000818"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0206166.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            19633..20946
                     /locus_tag="ACG8PO_000819"
     CDS             19633..20946
                     /locus_tag="ACG8PO_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFQ0206167.1"
                     /translation="MNHFWGRRLLTRDIKAIDCEEVNLPNYEKIAAVERLGNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEIAKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKML"
     gene            20943..21662
                     /locus_tag="ACG8PO_000820"
     CDS             20943..21662
                     /locus_tag="ACG8PO_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HFQ0206168.1"
                     /translation="MRCSCCSKDIVQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVKSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQVAQPKIIRTISLAR"
     gene            21790..22341
                     /gene="raiA"
                     /locus_tag="ACG8PO_000821"
     CDS             21790..22341
                     /gene="raiA"
                     /locus_tag="ACG8PO_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HFQ0206169.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADATALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            22563..25097
                     /gene="secA"
                     /locus_tag="ACG8PO_000822"
     CDS             22563..25097
                     /gene="secA"
                     /locus_tag="ACG8PO_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFQ0206170.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPHHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKSFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            25223..26324
                     /gene="prfB"
                     /locus_tag="ACG8PO_000823"
     CDS             join(25223..25294,25296..26324)
                     /gene="prfB"
                     /locus_tag="ACG8PO_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139124933.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFQ0206171.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRRSLDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            26429..27115
                     /gene="ftsE"
                     /locus_tag="ACG8PO_000824"
     CDS             26429..27115
                     /gene="ftsE"
                     /locus_tag="ACG8PO_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFQ0206172.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            27108..27992
                     /gene="ftsX"
                     /locus_tag="ACG8PO_000825"
     CDS             27108..27992
                     /gene="ftsX"
                     /locus_tag="ACG8PO_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HFQ0206173.1"
                     /translation="MIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFMAVI
                     MNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELKKIQ
                     NEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSDRIF
                     QIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRWPFF
                     LEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMVGIG
                     VIIGSLGSIISMRRFLKF"
     gene            28144..29592
                     /gene="cls"
                     /locus_tag="ACG8PO_000826"
     CDS             28144..29592
                     /gene="cls"
                     /locus_tag="ACG8PO_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFQ0206174.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWVLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHSTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            29929..30786
                     /locus_tag="ACG8PO_000827"
     CDS             29929..30786
                     /locus_tag="ACG8PO_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HFQ0206175.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVLQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            30901..31821
                     /gene="pstC"
                     /locus_tag="ACG8PO_000828"
     CDS             30901..31821
                     /gene="pstC"
                     /locus_tag="ACG8PO_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HFQ0206176.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            31821..32705
                     /gene="pstA"
                     /locus_tag="ACG8PO_000829"
     CDS             31821..32705
                     /gene="pstA"
                     /locus_tag="ACG8PO_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HFQ0206177.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            32720..33526
                     /gene="pstB"
                     /locus_tag="ACG8PO_000830"
     CDS             32720..33526
                     /gene="pstB"
                     /locus_tag="ACG8PO_000830"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002408906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFQ0206178.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            33542..34300
                     /gene="pstB"
                     /locus_tag="ACG8PO_000831"
     CDS             33542..34300
                     /gene="pstB"
                     /locus_tag="ACG8PO_000831"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFQ0206179.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            34313..34990
                     /gene="phoU"
                     /locus_tag="ACG8PO_000832"
     CDS             34313..34990
                     /gene="phoU"
                     /locus_tag="ACG8PO_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0045936 - negative regulation of phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HFQ0206180.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            35567..36259
                     /locus_tag="ACG8PO_000833"
     CDS             35567..36259
                     /locus_tag="ACG8PO_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HFQ0206181.1"
                     /translation="MLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLLNISIL
                     FVVIGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYL
                     TRPYRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVI
                     GSMTNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPIS
                     VLFFLTVRRLKR"
     gene            36256..37023
                     /locus_tag="ACG8PO_000834"
     CDS             36256..37023
                     /locus_tag="ACG8PO_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HFQ0206182.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            37123..38691
                     /gene="liaX"
                     /locus_tag="ACG8PO_000835"
     CDS             37123..38691
                     /gene="liaX"
                     /locus_tag="ACG8PO_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HFQ0206183.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEIAQVQL
                     STTTGSIYLKDTDK"
     gene            38988..39308
                     /locus_tag="ACG8PO_000836"
     CDS             38988..39308
                     /locus_tag="ACG8PO_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HFQ0206184.1"
                     /translation="MMKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVA
                     FGSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDE
                     WSDF"
     gene            39308..39658
                     /locus_tag="ACG8PO_000837"
     CDS             39308..39658
                     /locus_tag="ACG8PO_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFQ0206185.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            39825..40763
                     /gene="hprK"
                     /locus_tag="ACG8PO_000838"
     CDS             39825..40763
                     /gene="hprK"
                     /locus_tag="ACG8PO_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HFQ0206186.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            40778..41608
                     /gene="lgt"
                     /locus_tag="ACG8PO_000839"
     CDS             40778..41608
                     /gene="lgt"
                     /locus_tag="ACG8PO_000839"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFQ0206187.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            41652..42677
                     /locus_tag="ACG8PO_000840"
     CDS             41652..42677
                     /locus_tag="ACG8PO_000840"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFQ0206188.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGIGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            42692..43630
                     /gene="galU"
                     /locus_tag="ACG8PO_000841"
     CDS             42692..43630
                     /gene="galU"
                     /locus_tag="ACG8PO_000841"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFQ0206189.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYILSLGKTLEAEGSKIDHNPKGITEIKEEKKK"
     gene            43755..44195
                     /locus_tag="ACG8PO_000842"
     CDS             43755..44195
                     /locus_tag="ACG8PO_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HFQ0206190.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIATKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            44198..44800
                     /locus_tag="ACG8PO_000843"
     CDS             44198..44800
                     /locus_tag="ACG8PO_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HFQ0206191.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSTELTGIAKEKAQDLSQQAGDLAGSVKQKVSESADEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDMKKPADELGSIAKDTSEDIMIDVKDAAGKAGDTVNEG
                     MSEAKPVADDSKEMMKMAKEDAKEAKKEIKDTMTDSSTNH"
BASE COUNT        14887 a         7698 c         9764 g        12473 t
ORIGIN      
        1 taaaagatat gcagagaaag gattcaacgt tcttgtacct gatttacgtg cgcacggaga
       61 aagtgaagga gaaatcatcg gaatgggatg gctagatcgg ctagatttaa ttgcctggat
      121 ccaactcatc ttagatgaac agccgaatgc gtccattatt ttacacggcg gatcaatggg
      181 agcttctacg atcatgatgg caagtggtga aaaacttcca tcagcggtca aaggatttat
      241 tttagacagc ggatatgttt ctgtttatgc agaattccga tatatgttaa gtaaaatcac
      301 tgtctttcct aaaaaaatgg tcatgagata tgcgaatcat tatgcccaga aatacgccgg
      361 ttactcctta aagcaagcat cagctacgag acagcttggc agcaaccatc tgcctttatt
      421 agtcatccat ggtgaaagag atcattttgt ccctacagaa gcagcgtaca cgattcaaaa
      481 tgctacggca ggggataaag cactgctgct tgttccagaa gcagaacact tggaagcatc
      541 gatgaaagat ccgaaaacct attggacagt gattttttca tttattgaac aaagaataac
      601 gatggattaa ttttctcttc ttttttgtaa gtaattcgag gataaataat tgtttataga
      661 tctggagact gctaaaataa ggtaggaata aaaaaatatc agttatttga ggagtgcggc
      721 atatggaaat caaagaagaa aaaaatcgtc ttgctttatt caatgatgag caacaagaga
      781 ttggagaaat gacatggtca gatgcgggcc cagacatcat gatcattgac catacatttg
      841 tagatccagc atatcgtggt caaaagttag ctgaaaagct agtgtatact ggagtagaat
      901 tagcaagacg agacgggaaa aagatcattc ctttatgccc ttatgcaaaa aaagagtttg
      961 aaaagaaacc agaatatcaa gacgtcttac gtcagtcata agaataacag ctgaaagttt
     1021 tgaactttca gctgttattc catttatata gagttgtcac ggacttttat ctatgataaa
     1081 gttaaagaga cgatatcttt tgggaaggag gctgcgatat gtataattta attttgggaa
     1141 tcgtgatcgt tttatcgatt gttatggtca ttgcgattat gatgcagcca agcaagcaaa
     1201 atagtgcagc gagtgcattt accggcggtg cggatcaact attcggcaaa caaaaagctc
     1261 gcggctttga agctgtcatg cagcgatcaa ctgcagttat gggagctatc tggatgatct
     1321 tattatttgt cctagcatat ttatcttcga agtagttgaa taagcctctc gcaaaacggc
     1381 gggcggcttt ttttattgca aagagaacgc ttaagaagaa gttatgaaag aaactatggt
     1441 aaaatgaggc gaaagaggtg agacgagtga gacaattacc aaaacccctt tatgcaaaac
     1501 atggaaagcg agcggttttg ctgcttcacg cttactcagg aagtccaaac gatgttcgaa
     1561 tgctcgcacg atttttggaa aaagcagatt atacagttta tgcgccgctt tttaaaggac
     1621 acggaacaat ggagccatat gatatcttgc aagaaaaagc agaaagctgg tgggcagaca
     1681 ctaaaaaagc catccatttt ttacagtctg agcagttttc tgatattgcc gtattaggat
     1741 tatcaatggg cggcatcttt gctgtacgag cactagaaga agaatcagtg attggcggcg
     1801 gttttttctg ttcgcctttg tctcctgtaa aaacaaacgt gcctgaaaac tttgaaaaat
     1861 acgtacgtca agtgctgaag acagcaggta aatcagagaa agaaatcaat gaaaaagcag
     1921 ttgcttaccg ctcgttagct gaacaacaat taatggatat acaagatcag gcagcaatcg
     1981 tagaaagtag gttgtcagat atccaacagc cgatattttt agctcaagct gggaaagatg
     2041 aaatgatcga ccctaatggc gtatttgaaa ctgcacgcaa gttgagccgt cagcgagtga
     2101 cacttcaatg gtatcctgaa agtggtcatg tgattacagt tggtacagca agacgcgaat
     2161 tagaaaaaga cgtcttggaa tttttagaaa aattaccttg gaatgaggaa taaatatgac
     2221 aaaagaaacg ataaaagaga aaattctctt tttcatggaa agcagtcgca aacgcagttt
     2281 ttctatggaa gaaatcgctg aaggtttagg gttccaaaaa agcgatgatt ttaaattact
     2341 agtacaaacg attgctcaaa tggaaagaga acagagtata gttttcaata aaaaaggaaa
     2401 agtgaaactt cctttaaaac caatcttgat cgaaggaaca tttcgagcaa atgaacgagg
     2461 atttggattt gtaacgattg atcccgaaga agatgacatc tatatcccaa aagaagcgac
     2521 tggttttgca atggatggag acaccgtagc aatcgatatc atgaagcgag caaatacaga
     2581 tatggatcgc ggagcagagg ggaaagtagt cgagattcga gaccgggcga ctactcagtt
     2641 agcaggggaa ttcgttgctt attcagaaga agaaatacaa gaaacagatc tttacggcgt
     2701 tgttatcccg aaagatagaa agctcaacca atttaaagta tatgcagcag cagagggagt
     2761 tcgtccagtt gacggaagta ttgtgctgat cgaactgacc cattaccctg aaaaagggta
     2821 tgcgactagt ttagaaggga taatcaagca agtgattggt cataagaatg accctggcat
     2881 ggatatcctc tcaattgtcg tggctaatgg tatccctacg aaatttcctg atgatgtatt
     2941 gtctgaagca gatgaagttc cagatcaaat cagcgaaaag gatataactg gacgtagaga
     3001 tttaagaaat cagctcattg ttacgattga tggagaagat gcaaaagatc tagatgatgc
     3061 agttacggtt aaaaaattag ataacggtaa ttattttctt ggagtacaca ttgcagatgt
     3121 ttcttactat gtgacacaag gcagtcaatt ggacagagaa gcatatgaaa gagggacaag
     3181 tgtatatttg actgatcgag tgattccgat gatcccgcaa cgtttatcga atggtatctg
     3241 ttcactgaat ccccatgttc caaggctgac catgagttgc gaaatggaga ttgatccaaa
     3301 tggacaagta gtctctcatg atattttccc aagtgtgatc caaacaacag aacggatgac
     3361 ctacacagct gtcaatcaaa tcttagaaga tcaagacgaa gaaacgatgc aacgctatgc
     3421 tcagctcgta cctatgttcc aagaaatggg tgaattgcat cgaatccttg aaacgatgcg
     3481 aatcaaacgt ggcgcgattt cttttgagga tcgcgaagcg aaaatccttg ttgatgctga
     3541 aggacaccca aatgacatcc agctaagaag cagaggaatt ggtgaacgct tgatcgaatc
     3601 tttcatgctt gcggccaatg aaacggtagc tgaacattat aataaattaa atctgccgtt
     3661 tatttaccga atccatgaac agccaaaaga agaaaaaatg cagcgcttct ttgattttgc
     3721 ttcagctttg ggcattctgg tccgaggaac gaaaggaacg atcacaccaa aagatttaca
     3781 gcatgtgatc gaaggagtag aagacaaacc agaagctgca gttatcaata ctatgttgct
     3841 aagaagcatg caacaagcac gctattcaga agacaatttt gggcattatg gcttagcggc
     3901 agattactac acacacttta catctcctat tcgacgctat cccgatctaa tcgtccatcg
     3961 tctgatccgt tcttacagtg aagataaaag tgaaagcaat caaagttcgt gggctgcgga
     4021 acttcctgaa attgcagatc atagttctaa gatggaacgt cgtgcagtag aaacagaacg
     4081 tgaagtagat tccatgaaga aagcagaatt catgatggac aaagtgggag aagaattcga
     4141 tgggatcatc agctctgttg tgaaattcgg cctgtttatc gaactgccga atacagtaga
     4201 aggattgatc catatcaatg aattgaaaca ggactacttc cattttgttg aaaatcattt
     4261 agctcttgta ggtgaaagaa ctggtctgac ttttaaaatc ggacaaaaag tgcgcgtaaa
     4321 agtagtcaaa gctgatccag atgaacgagc agtggatttt gaactcgttt ccgcagaaga
     4381 ggtcactgcg ttggaacgtc caaaagcacg caatggaaaa tctggaaaac gagaaggaaa
     4441 acaagcaggc aaacgctcag aaaaaaatgg caagaagaac aaacgatata gtgatagtaa
     4501 gcaaacgttc ggtaagaaaa aagggaaaaa gccattttac aaagatgtca aaaagaagaa
     4561 aaagaaaaaa taaaatgagt tggaggaaac agctatgcca aaaggcgagg gaaaactaat
     4621 tgcacaaaac aagaaagctc gccatgatta ttcgatcatc gacacgatgg aagcagggat
     4681 ggtcttgcaa ggaacggaga tcaagtcgat acgaaacagc cggatcaatc tgaaagatgg
     4741 atttattcgc gtccgcaacg gggaagcttt cttgcataat gttcatatca gtccttatga
     4801 acaaggaaat atttttaatc atgatccgtt gcgcacgaga aagttattat tacacaagaa
     4861 acaaatcgtt cgtcttgaaa acgaattgaa aaacactgga atcactgttg ttcctttaaa
     4921 agtctatatt cgtaacggct atgccaaggt attgattggt ctggcaaaag ggaaaaaatc
     4981 gtatgataaa cgggaagatt tgaaacgaaa agatatcgat cgacaaattg atcgaacatt
     5041 aaaaaatttc tctagataat ttcattatcc tttttcgcat attcggtgct tttgctttta
     5101 cttaatgata aaatttgtta gcatttaatc tgataaaaca tgattatcac aggttttttc
     5161 ttagatcagg agcaccgtgc atggggaaaa attcgcttcg tttttgactg aaataaaaaa
     5221 gtgtctaatt ttcatgaaaa tgttttgaat tttctgtttc atggtggtat gataccgtgt
     5281 gggtagaaac cgattggaaa aaaccagata cgcacatgaa ttgattttat gtgaatttcg
     5341 gggaaaagag gtgaaatgga ttggatgaac gctcgctaac gtttcacatt ggaccaattt
     5401 ggtttgatgg tacagtttgt ataatggtgt tactgacctg tttgattgtt ttcttcttag
     5461 cttacttttt tacaagaaat cttaagttga aaccaacagg caaacaaaat gcgatagaat
     5521 gggtcattga ttttagtcga ggaatcgtaa cagataatct gcctagaaaa gaattgagca
     5581 atttccattt gttggcattt acattgtttt tatttgtttt tgttgcaaac aacataggtt
     5641 tagtaacaaa aatcgtctta cctaacgaaa caacactttg gaaaagcccg acagcagacc
     5701 cgtttgttac tttaactttg gcatttataa tgatcttact tactcactta ttcggggtga
     5761 aaaagttagg cttcaaagga tatttcgtaa attcgttctt gaaaccctat agttttatgt
     5821 ttccgatgaa actgattgaa gaatttacaa atttattaac acttgcttta cgtctttatg
     5881 ggaatattta tgcgggggaa gttttgctaa ccttgattgc aaaaatgatg acaagcttag
     5941 gctggttctc attaccatta gcaattccat tagagatggt ctggatcgcg ttctccttgt
     6001 ttattgggag catccaagca tttgtctttg tcactttgtc aatggtttat atgagccata
     6061 agattgaagt agaagaataa tactcggtta aaaaacaaac tactatttat ctattttagg
     6121 aggaaaacaa ttatgaatta tatcgcagca gcaatcgcaa tcatgggagc agcaatcgga
     6181 gccggttatg gtaacggtca agttatctct aaaacaatcg aatcaatggc tcgccaacct
     6241 gaaatgtctg gtcaattccg tacaacaatg tttatcgggg tagctctagt tgaagctgtt
     6301 cctatcttag gggtagttat cgccttgatt ctagtttttg ccgtttaatt gaatcagtat
     6361 taaggcagaa acaacatcgt ttgttactgc ttttttacat ggaaactaga aatactccaa
     6421 agaaaggaag gctcgcctat gctgaatcaa ttggcaattg cagaagttgg caatgcgatg
     6481 ttaggtaata tcatcgttgt cagcggctca tttttgatac tactggctct cttgaagcac
     6541 tttgcttggg gaccaatcag cgatattttg aaaaaacgtg aagacaagat cgccaatgat
     6601 ttagattctg cagaacaatc tcgcatcaac tcagcgaaaa tggaacaaga acgcgaacaa
     6661 caattgttag cctctcgttc tgatgcagct gatatcatca aaaatgcgaa agaaagtgga
     6721 gaattaagcc gccaaaatat tttgaaggat gctcaagaag aagcagctcg tctaaaaagc
     6781 aaagcccaag ctgatatcac tgtagaacgt gattcagcgc tgaactctgt aaaagacgac
     6841 gttgcagaac tctctcttca aatcgcggaa aaaatcttga acaaagaatt atctccagaa
     6901 atgcatgaat cattaatcaa tcaatacatt gaaggtctag gttcttcaaa tgaaactaga
     6961 taaatataca gtaggaagac gttacgggaa agctttattt gaactggcga tcgattcaaa
     7021 tcaagcagaa gaaatctatc aagatctcgt aagtttacgt caaatctatg aacaagtacc
     7081 tggattaggg aatatgttaa gtgatgttcg tctagaacct catgaaaaac gagaaatcat
     7141 ggacaagctt gtcagcggtt atgatgggat cgttaaaaac tttttagaag ttgtttatga
     7201 gtataaccga atggatgacc ttttgttcat gatcgaagaa tatgaacgtc gatatgatga
     7261 acatcaaggg ttactattgg gaagtgtcac aacagcagtc ccactttcag acgaacaacg
     7321 aatacaatta gaaaagaatg ttgcaaaaac aatggactat caatcagttg aattaaaaca
     7381 aatcgttgat tcttctatta ttggtggcgc gattgttgaa gccaaccatc gagtaatcga
     7441 cggaagcatt cgcacgcaat tagaaaaaat gcgtaatcaa ttaaacagat agtacaaaga
     7501 ggtaggtgaa tcgaatggcc atcaaagcag aagaaattag tgccttgatt aaagaacaaa
     7561 ttgaaaatta tcaaaacgtg ctttcagtcg aagaaatcgg taccgtaact tatgtaggtg
     7621 acgggatcgc tcgtgcacat ggtttagaaa acgcgatgag tggtgagttg cttgaatttt
     7681 caaacggctc atacggaatg gcgcaaaact tagagtcaaa tgatgtaggt atcattatcc
     7741 ttggcgattt cgagtccatt cgtgaaggag ataaagtaaa acgaacaggt aagatcatgg
     7801 aagttccagt tggagaggcc ttgatcggtc gggtagtcaa tccgctaggt caaccaatcg
     7861 acggactagg tgaaatcgtt actgacaaag ctcgtccagt agaagcgatg gcaccaggtg
     7921 ttatgcaacg taaatctgtt aacgaaccaa tgcaaacagg gctaaaagcg atcgacgccc
     7981 ttgtgccaat cggacgcgga caacgtgaat tagtcatcgg tgaccgtaaa acagggaaaa
     8041 cttctattgc gatcgatacg atcatcaacc aaaaaggtca agatatgatc tgtatctatg
     8101 tagcaatcgg acaaaaagat tctacagttc gtacacaagt tgaaacattg aaaaaatatg
     8161 gcgcaatgga ttacacaatc gttgtgaatg ccggtgcgtc tcaaccagca ccattgcttt
     8221 atatcgcacc atatgctggt actgcaatgg gtgaagaatt catgtacaac gggaaacatg
     8281 tattgatcat ttttgatgat ctttcaaaac aagctgttgc ttatcgtgaa ctttccttgc
     8341 tacttcgccg tccaccaggt cgtgaagctt atcctgggga tgttttctac ttgcactcac
     8401 gtttattaga acgtgcagca aaattaagtg atgatttagg tggcggttca atgactgcct
     8461 taccatttgt tgaaacacaa gctggagata tctctgctta tatcccaaca aacgttattt
     8521 cgatcactga tggacaaatc ttcttagaaa gcgacttgtt ctacgcaggt actcgtccag
     8581 cggttgatgc cggactatct gtatctcgtg tagggggttc tgcgcaaatc aaagcaatga
     8641 aaaaagttgc tgggacttta cgcctagact tagcaagtta ccgtgaatta gaagcattta
     8701 cacaattcgg ttctgacttg gatgcagcaa cacaagcaaa actaaatcgt ggacgccgta
     8761 cagtagaaat cttgaaacaa aaattgcatg caccgctagc tgttgaaaaa caagtagtga
     8821 ttttgtatgc tttgacacat ggattcttag atagtatccc tgtagacagt attttagact
     8881 tcgaacatga attattcgaa tatctggata ccaatcattc agatatcttt gaaacaatcc
     8941 gtacaacaaa agatcttcca gaagaagaag cgttgaacag tgcgattcag gaatataaag
     9001 acatgttctt agctacaaaa ggaaataatt cctctacaga agataaattg aaatccattc
     9061 aaaatgcata gtgaggtgag atgaatgggt gcttcattaa atgaaatcaa gacacgtatc
     9121 gcttcaacga aaaaaacgag ccaaatcacg cgggcaatgc agatggtgtc tgcttctaag
     9181 ttgacaaaat ctgaagcttc ttcacagaag tttcaggttt atgcaacaaa agtaagagaa
     9241 atcgttacgc atttgactgc tactcagtta aatgatattg cctcagaaaa tcctagaggg
     9301 gatatcaatt acaacagcat gttgctcagc cgtcctgtta agaaaacggg ttatatcgtc
     9361 atcactgcag atggcggttt agtcggtgga tataacagct cgatattgaa acaaacgatg
     9421 tctattttag aagaagacca ccaatcaccc gatgattacg tgatgatcgc aattggcgga
     9481 actggtgcag acttctttaa agcacgcggt atcaatctag catatgagct gcgcaatcta
     9541 tctgatcagc caagctttga tgaagtccgc aaaatcgtaa atatggcaac aactatgtac
     9601 caaaacgaag tattcgatga attatacgta tgttataacc atcatatcaa ttcgctaact
     9661 agtcagtttc gtgtagaaaa aatgctgcca atctcagact tagatccaga agaagcaact
     9721 actttcgaac aagaatatat ttttgaacct tcaaaagaag aaatcttgtc tcagttattg
     9781 cctcagtatg ctgaaagttt gatctacggt gcgatcgttg acgccaaaac agcagaacat
     9841 gctgctggta tgacagcaat gaaaacagca actgataatg ctgctaatat cattgatgag
     9901 ctgacggttt cttacaaccg tgcaagacaa ggggctatta cccaagaaat taccgaaatt
     9961 gttgccggag cttcggcact agaatagtat tgagattgga ggaaaaaaga tgagttcagg
    10021 caagattgtt caagtaatcg gtcccgttgt cgacgtggaa ttttctttag accaatcctt
    10081 accagatatc aacaacgcat tagttgttta taaaaatgac gaaaataaat caaaagttgt
    10141 tcttgaagca gcgttagaat taggggacgg agtgatccgg accattgcga tggaatcaac
    10201 agatggttta caaagaggaa tggaagtcat tgatacaggc aaagcaatct ctgttcctgt
    10261 aggtaaagaa acattaggtc gcgtattcaa cgtactagga gatacgatcg atttagaaac
    10321 acctttccca gaagatgcgg aaagaagcga aattcataaa aaagcaccag cctttgacga
    10381 attaagtaca agtacagaaa ttttggaaac agggatcaaa gttatcgatt tgcttgcccc
    10441 atatttaaaa ggtgggaaag tcggactatt cggtggtgcc ggtgttggta aaaccgtact
    10501 gatccaagaa ctgatccata atatcgccca agaacatggt ggtatttctg tatttaccgg
    10561 tgtaggtgaa cgtactcgtg aaggtaatga cttgtattat gaaatgaaag attcaggagt
    10621 tatcgaaaaa acagccatgg tgttcggaca aatgaacgaa ccaccaggtg cacgtatgcg
    10681 tgttgctttg actggtttga cgattgcgga atatttccgt gatgtagaag gtcaagatgt
    10741 actgttgttt atcgacaaca ttttccgttt cactcaagct ggatctgaag tatcagcctt
    10801 gttaggacgg atgccttctg cggttggtta tcaaccaaca ttggcaacag aaatgggtca
    10861 attgcaagaa cgtatcacat ctacgaaaaa aggttcgatc acatcgatcc aagcaatcta
    10921 tgtgccagcc gatgactaca ctgacccggc gccggcaaca gcattcgccc atttggatgc
    10981 gacaaccaac ttggaacgta aattaacaga acaagggatt tatccagcgg tagatccatt
    11041 ggcttcttct tcaagtgctt tagcacctga aatcgtggga gaagaacatt ataaagtagc
    11101 aacagaagtc caacacgtat tgcaacgata tcgtgaatta caagatatca ttgctatctt
    11161 aggtatggat gaattatccg atcaagaaaa agtgttagtt tctcgtgccc gcagagtcca
    11221 attcttcttg tcacaaaact ttaacgtggc tgaacaattt actggtcttc caggttcata
    11281 tgtccctgta gaagaaacag tgaaaggatt ccgcgaaatc ttggaaggca aatacgatga
    11341 tcttcctgaa gaagctttcc gaagtgttgg ccgaatcgaa gacgtcgtag aaaaagcgaa
    11401 aacactaggc tactagaaag gggtgcccca tggatcaata tttaacagta aatgtggtaa
    11461 ctcctgccgg tttggtctat gatcaccatg cagctatcgt cgttgcacgt acgacagctg
    11521 gtgagcttgg gattttgcct aaacatgcac caatcatcgt tccattgacg attgatgaag
    11581 ttcgtgtaaa acgaacagat tcagataccc acgtcgattg gatcgctgtt aatggcggaa
    11641 tcatggaagt tcgtgataat gttgtttcaa tcgttgctga tagtgcggaa cgtgaacgtg
    11701 atatcgatgt atctcgtgcg gaacgtgcaa aacaacgcgc agaacgccaa atcgaagaag
    11761 caaaagcaaa acaagatacg aacgaattga aacgggcaac tgttgcgttg caccgagcaa
    11821 tcaatcgtat caaagtatct aagcactcat aaaatgagat gcggatgttt atccgtgttt
    11881 aagaacagta aaaagaggtt atgacagaag tggtcagctt caagacataa gaaggaattt
    11941 acgaaaattg cttctcaaat ttttgtgaaa tctcagctta tttttcgaag aagctacttc
    12001 tggaacaccg tatatcggag gttgtgaaat cagcgttttg tcctgagtat tagagaaaca
    12061 aatggaaaat agttcctcat attttgccgt tttttgagct aataccgagg gtcagatgat
    12121 tgaacaccgt ttatttggaa taagggactg cgacaagact tttgtctcag tccctttttt
    12181 tgcatatttt tagcatttat ttcctaaaaa atgtaagggg attcctatat ttttaagtat
    12241 acttgttttt ccttttatac attttatggt attatcattt tattagttat tataaaacaa
    12301 tcgttagaaa ggacgtcatt atgcaagtat acggaattga tgcggtgctt cgaatcgtga
    12361 gtcatttggc gtttatctac ttagccttct ggtctttacg ttcgttacgc attgaaactt
    12421 ttttcagacc aatgcataat acgcaaatta gaatggcaat cgtacttttt tcaatttttt
    12481 tagggtacac tgcaagtaat tttttcttag aagttatcgc tttatgccga aatcttttcg
    12541 ttagcctgta aattttaaaa aaaggtacta ttttaaaagc aaggatgtta taatagaaaa
    12601 gattttgagt aagtgtatgc tcattggaat tttttggagg gaacaacatg gaagagatca
    12661 tcgtaagagg tggcaatcaa ttaaatggaa cagtaagaat tgaaggagct aaaaatgctg
    12721 ttttgcctat tttagctgct agtttgttag cagaagaagg tattacgaca ttagacaacg
    12781 ttccgattct ttctgatgtc ttcactatga atcaagtgat tcgtcactta aacgtagatg
    12841 tagattttga tgaacaaaag aatcaagtaa caatcgatgc ttcaagacaa ttagaaatcg
    12901 aagcgcctta tgagtatgtt agtcaaatgc gtgcttcaat cgttgtgatg ggtccgctac
    12961 ttgccagaaa tggacatgca aaagtagcta tgcctggtgg atgtgcgatt ggtaaacgac
    13021 caatcgattt gcatttaaaa ggatttcaag ctttaggagc aaaaattatt caaaaaaacg
    13081 gctatatcga agcaattgcg gatgaattga tcggtaacac gatttattta gatttcccta
    13141 gtgtaggtgc tactcaaaat atcatgatgg ctgccgtaaa agcaaaagga acaaccatca
    13201 ttgaaaacgt agctcgcgaa ccagaaatcg ttgatttagc aaacattttg aataaaatgg
    13261 gcgctcaagt ctatggagct ggtactgaaa caatgcgtat tgaaggcgtg gatcatttac
    13321 atgcagtcaa ccactcaatc gtacaagatc gtatcgaagc aggtaccttt atggttgctg
    13381 ctgcaatgac acaaggcaat gtgctgattg cagatgctat ctctgaacat aaccgcccat
    13441 tgatttctaa attgatcgaa atgggtgcag aaatcattga agaagaaggc ggagtccgtg
    13501 tcatcggacc aaaacatatc ttacctacag atgtaaaaac gatgcctcat cctggtttcc
    13561 ctacagatat gcaagctcaa atgacagcta tccaattagt tgcagaaggt acaagtgtag
    13621 tgactgaaac agtgtttgaa aatcgtttcc aacatttaga agagatgcgc cgtatgaacg
    13681 ctcatgtcaa aatcgacgga aatgtagcaa tcatggatgg aaaccatgaa ttgcaaggtg
    13741 cagaagtata tgcaacagac ttgcgggcag cagcagcatt agttttagct ggcttgaaag
    13801 caaatggaat cacacgtgtg cgtaacttga attatctaga ccgtggatat tataatttcc
    13861 atatcaaact acagcaatta ggtgcagacg ttgaacgtgt agacatggat caaacttctg
    13921 ctgaaaaaac agcacaaacg attgcttaag gaagtggctg aatgagtaac caacgatata
    13981 tcttattaac tttgataaag attttagtgg tgatcttatt gcttatcctt ctttttgtgg
    14041 ccggaacgat gatcggatat ggtgtcatcg gtggcggcaa tccatttaaa gtattccagc
    14101 caagtttgtg gattcatatt cgagactttt ttcattgata aataagaatc acagtggttc
    14161 tagtcgatat gaagtgagcc atgaagcaga acatgacaaa agaaaacaga agggaacgtt
    14221 gacgttagtc gcgatccctt tttttagtaa aagaaaagaa ggaatctaat tgatgaagaa
    14281 gatattgatt tatttagtta gaggatatca gcgctttatt tcgcctgcat ttccacctag
    14341 ctgccgttat tacccaacct gttccaatta tatgataaaa gcaattcaaa cccatggtgc
    14401 actaaaaggg acggtaatgg ggactgcacg gatcttacgc tgtcatcctt ttgtcaaagg
    14461 cggtatcgat tatgtaccat tgacattcca attaacgcga aataagaaag ataaaaaaga
    14521 accagattat aaaaaggttg tgaaaaatac gagtagcact gagcgataaa gaaacaattt
    14581 ggaaaatagc ttcttatatt ttgcaaaatt gtgagttatt gccgaagtgt tggattttga
    14641 acaccgtcaa aaaaaggttg tgaaaaatac gggtagcact gagcgataaa gaaaaagagg
    14701 aaaattattg tcaaagtaac aactccgcaa ctacttatat gggggacaaa aaatgatcga
    14761 agaactaaac aagaaagatt taccatggtc cttgttatta gatgcagatc ctgataaaga
    14821 aaaagttcaa gtatatgttt ccagagggag tggctggata tggaaagaaa agaaaaaaca
    14881 ataggtgtac tgatctatgt ggcaagggaa aaagaatacg aaatcgtcaa tgtagcagta
    14941 gccccaacgt atcaaggaaa ggggattggc ggaaaattac ttgagacagc ttttcagaag
    15001 ctttcacaac tcgcttccag tcaaactcag atcatcatac gaaccggcag tactagcagt
    15061 gcggcgttac atctctatca aaaaatgggc tatgtggaga ttggtcgagt caaagactat
    15121 tttatccatc attactctga gcccattttt gaaaatggag atcaattaag ggatcaagta
    15181 attttagcaa ttcacttacc taaaaatagc tttaactaat aaactttcaa ttcgagaaaa
    15241 tgctttcaaa tgctaaatag atacattaca atagctttag caaacaaaaa ggagaatatt
    15301 tttatgggaa tattagtttt tgattttggt ggatcggctg tcaaatatgg ttgttgggat
    15361 ggcaaagaga ttaaaggtaa gggacaattt gcaacgcctg aatcttggga agaaatgaaa
    15421 gcgcaattat ttcaagttta taaaaaaata ctcgtttctt ttgaagtcga aggaattgga
    15481 atcagctctc ctggagtagt gaataatgag aaacaggtga ttgagggaat cagtgcgatt
    15541 ccttatattc atggatttaa tattttccga gatttggaaa ctctgtttca actcccagta
    15601 acgattgaaa atgacgccaa ctgtgccgga atggctgaat tttatgaagg tgctgcccgt
    15661 gattatcaag atgttgcttt tgttgtggca ggaactggga ttggcggggc gttgttcacg
    15721 aaaggacaaa tcaataaagg ggcacatctt tatggtggag aatttgggtt gatgttctta
    15781 aatggcgata agacattcag taaacttggt acagcggtac agatggcttg gcgttattgt
    15841 gaacgcaaag gtctcgataa aaattcttat accggaaaag atgtctttga attagcagaa
    15901 actggagatg caatagcaaa agaagaagtt gagagttttt atacgtatct tacaaaggga
    15961 ttattcagta ttcaattttc gtttgatcca gaagtgatcg tattaggcgg cggtgtttct
    16021 gcaaaagaag ggctcattga tgagatcaaa agccgaatga agaagctgac agaacaattc
    16081 gatctgcatg atttcgaacc gcagatttta ttatgtgaat acagaaacga tgctaatctt
    16141 gtaggtgcag cagcaaacta tctcgctgta cgtcaagggg agaattaaga gctttagaga
    16201 aggtgcagct tgcataaaag aagtgaatga aatgaatagg agatttttac ctaaggaatg
    16261 ttttatttgc tgcagtatga taaaatatct gagaggtgaa aatgatgtca aaaaaaaata
    16321 aagatgttga agtgcgtata gaagaagtga aaaaagatat caaaggtaag aattatgaag
    16381 tcactcagtt atttataggg aaaaaaatga ttggtgagat cttagcctat gggccaaaag
    16441 aatatgaaat tttctttggt gaagaagatt ttggaaaaga aaaaagtctt gaaaatgcga
    16501 tagaaacagt gattcgacac tggaatctgc acgaataaaa aaatatcaaa aaaactttca
    16561 ataaagggtt gcaaaaatct ggaaaattag gtattataac taagtcaggt tgatacaaac
    16621 ctttcggagg agtagcgaag tggctaaacg cgacggactg taaatccgtt ccttagggtt
    16681 cagtggttcg aatccactct cctccatttc attggggtat agccaagcgg taaggcaagg
    16741 gactttgact ccctcatgcg ttggttcgaa tccagctacc ccagtatacc acaatcgtaa
    16801 gctctcgtta gttcgagagc tctttttttt gctttgctac cttgttcctt gaagattctg
    16861 ctgatttaag atagagttaa gttaactaca agaattggag ggatccacat gaaatggaaa
    16921 gaaaaagtag acgaagcagc tgaaaagtta tatgatttgg tcaaatcaga aaaatacaat
    16981 attgaagtga atattccgaa aaaaggcgga aaagcggtcc aggtaaaatc aaaacgccca
    17041 acgaatcata caaaaaaatg gatggctaaa aaccgctaat aataacaaaa gatccataag
    17101 tcggcatttt catcgatttt agggtctttt gttattttct aggttgaaca tatctaaaaa
    17161 attgtttctg acaataggag taggaagaac gatgtgtgtc taaatcctct gttgatggat
    17221 agaaaccttt atctttttca gttgtgaatc aggtatatac cagttataat agaagaaacg
    17281 aaaggacggg tgtacgttat gtcgaatcaa ttacctgtat atatacagat tcatgaccaa
    17341 ttaaaaaatg aaatcgaaca aggtatatgg aaagtcggag accgtcttcc ttcagaaaga
    17401 gaattagcag tgaaattcaa tgtcagccgt atgacgttga gacaagcaat tcaaaacttg
    17461 gcagatgaag gaatcttaga aagaaagatc ggttcgggta catatgttgc gcgagaaaaa
    17521 gtacaagaaa aaatgtctgg tgcaacaagt tttacagaaa ttatggaatc gcaaaatcga
    17581 gtaccgtcta gtcggacaat ctcttatttc ttgacttcgc caagctcaag tgaaatggaa
    17641 aaactaaact tgagtaaaga tgacacgatc ttgcgaatgg aacgtattcg tttcgcagat
    17701 gatgtcccta tttgttttga agttgccagt gtgccacaaa agctgatcga tggctacagc
    17761 aaagcagaga ttactgaatc gttgtatcgc acgctagaag aaaaaggcgg gaaaaagatc
    17821 ggtccagcga atcaaacgat tactgctgtg ttggcttctg agaaaattgc tgactattta
    17881 gaggtaaaac gaggagatgc agttcttcgt ttgcgccaga tttcttattt tgaagacggt
    17941 acgccgtttg agtatgtgcg tacgcaatac gtaggaaatc gttttgagtt ttatttggaa
    18001 aaataaaaaa caggtgatcc ctgattcctt acttagaaat aaaagagaat cagaatcacc
    18061 tgtttttata tatgatcagt taccagttgc tcgtgataag aaacaggacc ctcttcaaat
    18121 tggacttgtc cgttcagcag ctcaatgatg gattctttaa agtggtcagc acttttttca
    18181 tctacagcac agatgacgct aacttgatca gtatattgga tttcttttat aaaaaaatgt
    18241 tggagttcaa gataattctg taattttcct agttgcggat acgtgatcat cacagagatc
    18301 tcctgttgca aggtaccttc tacgattcca atgtcagaca atgcttgaga gacagcatga
    18361 gtgtatgctc gtattaatcc gcctgtgccg agtttcgttc ctccaaaata tcgagtgact
    18421 actgcagcta cattgatcag ttgatttttt ttgaggactt ccaacatcgg tacgccagcc
    18481 gttccacttg gttctccatc atcgctgctt cgctggattt catttttttc tccaatgatg
    18541 aaggcgctgc agttatggtt cgctttccaa tgttcttttt ttatttcttg aatgaaactt
    18601 tttgcttggt cctctgattc tattcgtcta agagaacaga taaagcgtga ttttttgatc
    18661 tcaatctcac tttgtccatt ttcttttatt gtacgatagc gctcgatcat cttgtcacct
    18721 ctttttttca ttttctttat tcagtataaa taaagttcta ggttgtttca acactgtaac
    18781 ggttgcgcaa tcgctgtagt atgcgataat aaagacaaag gtaggtggat ctggggatgc
    18841 aggaacgaat gaaaaagtac gatgcaatta cgcattattt gaagaacaat ggcggctctc
    18901 aagtaacgtt gacctttacc caatttgacg aactgctgtt tcccagtaac ggacttccca
    18961 aaacagcaag ggaaagtaca gattggtggg caaatgatta taaacaccct gaaaaaggtg
    19021 cctatggctg gataaatgca ggctatgaag tagttgtcat caatttggac aaagaatatg
    19081 tcgtgttcaa taaattagtg aaatctagtt ggctatttga ttaaataaac cgcgtttaga
    19141 aaacgttgac aaatttcttt ctttttatat aatagagtaa cagacgcgaa gaaaacgttt
    19201 gcgtagtttg ttttaaaaag gagagaaaag tatggctaaa aaaacaatca tgcttgtatg
    19261 ttcggctggt atgagcacaa gtttactagt aacaaaaatg caaaaagctg ctgaagcaaa
    19321 aggaatcgac tcggatattt tcgctgtgtc cgcttctgac gcagataaca atcttgcaaa
    19381 taaagatgta gatgtactgt tattaggacc tcaagttcgc tttatgaaag ctgattttga
    19441 aaaacgtctg gaaccaaaag gcattccttt agacgtcatc aatatggctg attatggtat
    19501 gatgaatggc gaaaaagtgt tagatcaagc aatcacgctc ataggtgaat aaatagaaca
    19561 gtataactat cgaacaagcg atccaaaaaa tagatcgctt gttttttcgt atattttttt
    19621 agggcgaata atatgaatca tttttggggt agaaggcttc tgacgagaga tattaaagca
    19681 atagactgtg aggaggtaaa tttaccaaat tatgagaaga tcgcagcagt ggaacgtttg
    19741 ggcaactgga ttctttgtca tcgctgtgga atgaggacgt ccgtcattga tggacagctt
    19801 gcagattacg gttatttttg tacacattgt ctatatttag ggcgatgcga cactcaacaa
    19861 gatttatatt tgtttgatca gcctgaagca aaacctcgag aagtcgtctt tttttggaca
    19921 ggacagctta ctgaaaaaca aacggaaatc gcaaagagaa tactttgcca ttcagaaaaa
    19981 agacatcatc ttatttgggc tgtaacaggt gcagggaaaa cagagatgct ttatcctatt
    20041 ttagtggaaa ctttaaaagc tggaggaaga gtagcgattt gtacaccgcg agtcgatgtt
    20101 tgtaatgaat tgtttttacg ctatcgttat gtttttccag aagaaaaaat cacgttgctc
    20161 catgggaata cagcaacggc ttataccttt acttcttttg ttatctgtac gattcatcag
    20221 ctataccgtt tttatcggtc ctttgattta atagtgatcg atgagatcga tgcgtttcct
    20281 tataacgggg atgcaggatt agctcatgct gtgcaaacag cgattaaacc agacggacga
    20341 ttcatctact taagtgccac acctgataaa aagttactaa aggaaatcaa gcagacgttt
    20401 gacatccaca aacttgcttt gcgctttcat cggcgtttac ttcctgaacc cgtcttggtt
    20461 ttcatgaata attggcgaca aaaatgcaag aacaagaaga agataaaatc tttggtgcgg
    20521 atcattcatc aattaattaa agaaaaccat atccttttat tttgtccaag tatcacgatg
    20581 atgcatcgtt tgaaagccac acttcaagag gctcttccga gatatgagat cacagatgtt
    20641 tcttcaagag acaatcagcg ggcggaaaaa gtccagaaaa tgcgagaagc aaaatatgac
    20701 atactgttaa ccacgatgat ccttgaacgt ggagtcacat ttgaaagaat atccgttatc
    20761 gttttaggag cggatcatcc ggtttttact aaatcttctt tggtacaaat cgcaggaaga
    20821 gccgatcgta aagggcctta tacgaacagt caagtatact ttttctatga agaaaaaact
    20881 gcagctattc gaaaagcttg tcaggaaatc aaagaaatga atgaagaagg gaagaagatg
    20941 ctgtgaggtg cagttgctgc agtaaagata tcgtccaaaa tttaacattc agcgagatta
    21001 ttggtgtttc gaaatacaga ttacctcaga cttgttcagt ttgtcgcgca caatttcatt
    21061 tattggaaac aagaggctgt tctggctgtc aaaaaccaat ccatttcaat ctagagagta
    21121 cgaaaaagaa agtaaagtct attcccgttt actgcagtga ctgtcaagaa tggaagaaga
    21181 aatatccgac ctatgatttt cagcaccgtg ccttttttgt gtacgatgaa aatatgaagg
    21241 cctggctcaa tcaatacaag tttatgggag atatccgact agcaggcacc tttgcatata
    21301 aatggacgca gataaaaaaa gaatacccga catacatcta ttgtccaata cctttatctg
    21361 aaaaaaggcg aacagaaaga ggattcaatc aagtaagcga gatgttgaaa gcagcaagaa
    21421 ttccttttca gatgctactc aaaagagaga agcatctgct ggctcaagca aaaaacacgc
    21481 gaaaggaaag attggagatg cctcaacctt ttgaacttgc agtcaccttt tctaagatac
    21541 aaaataaaaa aatattgatt attgatgatg tctatacaac aggacagact cttttccacg
    21601 cagcagattg tttgcaggtc gcacagccga aaatcatccg aaccatttct ttagcaagat
    21661 gaattagatg tggaaatttg atagcgattt aatttgcaaa taacctagag ttcgatataa
    21721 tagaattaag aagagggaaa gagtggtcct tctccttctt gattgtggta gacgaaaggg
    21781 gtaattttta tgtttagata taatgtacgc ggcgaaaata tcgaagtaac tgaagcgatt
    21841 cgcgactacg ttgagaaaaa agtaggcaaa ctagaacgct atttcagtga cgcacccgat
    21901 gcaacggcgc atgtgaactt aaaagtttat acagaaaaaa cagcaaaagt ggaagtaacg
    21961 atcccattac cttatcttgt attacgtgcc gaagaaactt ctccagattt atacgcaagt
    22021 atcgatttgg ttgttgacaa attggaacgc caaatccgca aattcaaaac aaaaattaat
    22081 cgtaaatccc gtgaaacggg catgaatact gcagatgcta ctgctttatt caatgaagag
    22141 gacgaaaacg aaaatgattc tgaattagat atcgtacgta cgaaacgcct ttcattgaaa
    22201 ccaatggata gtgaagaagc agtcttacaa atgaacatgc tgggacataa cttctttatt
    22261 tttgaagatg cagaaacaaa tggtacaagt atcgtttacc gccgtaaaga tggaaaatac
    22321 ggactgattg aaacagactg atcagcctta cttaaataac aacgaaaact tgcagcagct
    22381 actgacagct gccgcaagtt tttttgagtt tcctcctctt ttagatgaaa tatgaataaa
    22441 ttatgaagtt cttactttaa tacgcttaag atatttcaat tatctataac taatgataaa
    22501 atgaaaagta aagtctacat agtctggata atacacagaa ggaaaaggag attacacaac
    22561 gcatggccaa tttgattcgt tcacttatcg aaaacgataa gaaagaacta aaacgtttag
    22621 atagcattgc aaaaaaagtt gaagcatacg ctgaccaaat ggcagcatta actgatgaac
    22681 aattacaggc aaaaactgct gaattcaagg gcagatacca aaaaggagaa acattagatc
    22741 aattacttcc ggaagctttt gcagtcgtcc gagaagcagc aaaacgtgta ttggggttat
    22801 atccacacca cgtacaattg atgggtggaa tcgtgcttca cgatggaaat atccctgaaa
    22861 tgcgtactgg tgaaggtaaa accttgaccg caacgatgcc agtatatcta aatgctctga
    22921 ctggcgaagg ggtacacgta gttaccgtca atgaatattt agcaacccgt gactcgacgg
    22981 aaatgggcga actttataat ttcttaggat taacagtggg gttgaatatc aactcaaaat
    23041 cttcggatga aaaacgcgag gcttataatt gtgatatcac ttatagtacg aataatgaat
    23101 taggatttga ctacttgaga gacaatatgg tcgtttaccg ccatcaaatg gtacaacgtc
    23161 cgctgaatta tgccattgtc gatgaagttg actcaatctt gattgatgaa gcgagaacgc
    23221 cgctgatcat ttcagggcaa gcagaaaaat ccacagcttt atatacaaga gcagacaact
    23281 tcgtgaaacg tttgaaagaa gaagaagatt ataaaatcga tgtacaatca aaaacaatca
    23341 gtttaaccga agcagggatc gaaaaagcag aagaaacatt cggtttggat aacttatacg
    23401 acattgaaaa tactgcattg acccatcact tggatcaagc attgagagcc aactatatca
    23461 tgcttttaga tatcgattat gttgtccaag acggaaaagt actgattgtt gaccagttca
    23521 ctggtcggat catggatgga cgccgttatt cagatggact tcaccaagca atcgaagcta
    23581 aagaaggcgt agagatcgaa gacgaaacga aaaccatggc gacgatcact ttccagaact
    23641 atttccgtat gtacaagaaa cttgcgggaa tgactggtac agctaaaaca gaagaagaag
    23701 aattcagaga aatttataac atccaagtat accagattcc gacaaatcgt ccggttatcc
    23761 gtgatgatcg tccagatttg ctttacccaa cattacaaag caagttccac gctgtcgtgc
    23821 aagatatcaa agatcgctac cgcaaaggtc aacctgtgtt agtcggaact gttgcggttg
    23881 aaacatctga gttattatca gatatgctga atcaagaacg tattcctcat gaagtgctga
    23941 atgcgaaaaa tcactttaaa gaagcagaga tcatcatgaa tgctggtcaa aaaggtgctg
    24001 tgacgattgc taccaacatg gctggtcggg gaacagatat caagttagga cttggggtaa
    24061 gagaacttgg tggtctagca gttatcggga cagaacgaca tgaatctcgt cgtatcgata
    24121 accagcttcg aggacgtgca ggacgtcaag gagaccctgg tatgtcacag ttctacttgt
    24181 cattggaaga tgatttgatg aaacgattcg gttcagaacg aatcaaatct ttcttagatc
    24241 ggatgaagat cggtgaggaa gatgctgtta tccaaagcaa gatgctgacg aaacaagtag
    24301 aatctgcaca aaaacgtgta gaaggaaata actatgacac acgtaaaaac gtcttacaat
    24361 acgatgatgt gatgcgtgaa caacgtgaag taatctataa acagcgacaa gaagtaatca
    24421 tggaagaaaa aagcttatcc aagccactaa tgaacatggt caaacggaca atcagtcgtg
    24481 tagtagatgc gcatacacaa ttagagaaag aaaattggaa tttggaaagt atcatcgatt
    24541 ttgccggcag tactcttgtc catgaagata gtattactct tgcagatatc gaaggaaaaa
    24601 cagctcagga gatcaaagag tatctaaacg aacgagcaca agaagtattt aataccaaag
    24661 cggctcaact aaatggtcca gagcaactgt tggaatttga aaaagtcgtg atcttgcgtg
    24721 ttgtggactc taaatggaca gaccatattg atgcaatgga tcaattacgt cagtcaatcg
    24781 gcttacgtgc ttatggacag aacaatccat tagtcgaata tcaaacagaa ggctataaga
    24841 tgtttgaaga tatggtaggc gccatcgagt atgaagtcac tcgaatcttt atgaaagctg
    24901 agatccgtca aaacgtacag cgtgaacagg tttctcaagg aacagcttct cactcagaag
    24961 acggagatgc caaagaaaat agtaatacta gcgcgaaaaa acagccaatc catgtgaaca
    25021 aggtcggaag aaatgatcct tgtccttgtg gaagcgggaa aaaatataag aactgtcatg
    25081 gaaaaaatgc aaactaattt ctgacagcag gattggtgag acaaattctt tttttaagaa
    25141 ttcgtctcac cttctttctg ttatttgctg attctgttag actagatata atgaaaaaag
    25201 tatagtgaaa gaaggaaaaa acatggaaat atccgaaatt cgaaatctcc tgacagaaat
    25261 gcaggaaaag gtcacaagct tcaggaggtc tctttgactt agaccagctg gaagaagaca
    25321 ttgcggaagc agaagcaaaa atggctgaac ctggtttttg ggatgatagc gaagctgctc
    25381 aaaaagtgat cagtgaaaac aatgcaaata aagaaactta tgatcaattc cattctcttg
    25441 ctgaagaact ggaagaaatc gaattgatgg tggagatgtt gcaggaagaa cctgatgcag
    25501 ccatgcaaga agaagcagaa acacgtatca atgcgctaga tgaaaaaatg aagacatatg
    25561 aattatctat gttgttagat ggtccttatg atcgaaacaa cgccatcgtt gaattgcatc
    25621 caggggctgg cggaacggag tcccaagatt ggggcagtat gcttttacgt atgtatacac
    25681 gctgggcaga gcagcatggg tatcaagtag agacactaga ttatcaagca ggtgatgaag
    25741 caggaatcaa gagtgtgacc ctactgatca aaggacacaa cgcatttggt tatctgaaat
    25801 cagaaaaagg tgttcatcgc cttgtacgta tctcgccttt tgactcagcg aaacgacgtc
    25861 atacttcttt ttgttcggta gatgtgatgc cagagcttga tgaaacgatc gatatagaaa
    25921 tcaatccaga tgatttgaaa atcgatacgt accgagcaag tggtgcaggt ggtcagcaca
    25981 tcaataaaac agaatctgct gtacggatca ctcacttgcc tacaggaaca gtagtagcta
    26041 gtcaggcaca acgttcacaa ttgaaaaacc gtgagcaagc gatggggatg ttaaaagcta
    26101 agctttatca attagaagtg gaaaagaaag aacaagaagc agctgcactt cgaggagaac
    26161 aaaaagaaat cggctggggt tcacagatcc gttcttatgt tttccatcca tattctatgg
    26221 taaaagacca tcggacaaac gtagagacag gaaatgtcca agcagttatg gacggtgacc
    26281 ttgatttatt tattgacggc tatttaaaaa tgcatctaca ataagaaccc attgttaaat
    26341 tgaaataaaa ttgtaagatt ttgcaagcat gttgaaacat tgccatgcta taatagctaa
    26401 agactgaaaa agatatggag agaacgccat gattgaaatg aaggatgtaa tgaagaagta
    26461 ctcaaatggt acgaccgcta tccgcaatct ctcaattgtc atcgaacaag gagagtttgt
    26521 ctatgtggtt ggtccgtctg gagccggtaa atctacattc atcaaattga tgtatcgaga
    26581 agaaaaagct tcaaaaggaa ctttgaacgt cgcaggtcat gatctaatag caatcaaaaa
    26641 ccgcgaagtt ccttatctac gcagagaaat cggcgtggtg ttccaagact ataaattatt
    26701 accaagaaaa acagtatatg aaaatgttgc gtacgctatg caagttatcg gcagaaaacc
    26761 tcgggatatc aaaaaacgag taatggaagt gctggacctt gttggtttga aacacaaagt
    26821 tcgagtcttt cctagcgaat tatccggtgg ggaacagcaa cgtgtctcta ttgctcgggc
    26881 aatagtgaat acgcctaaag tacttatcgc ggatgaaccg acaggaaatc tggatccaga
    26941 aaactcatgg gaaatcatga aattattaga tcggatcaat gcccaaggaa ctactgtcgt
    27001 gatggctaca cataacagta cgatcgtaaa tacgattcgg caccgagtaa ttgctatcga
    27061 aaacggccgc attattcgag atcaagcgga aggggaatat ggatacgatg attagaacct
    27121 ttttccgcca tttattagaa agtatcaaga gtttgaaacg taacggctgg atgacagttg
    27181 cttcagtcag tgcggtaacg atcacattga ctttagtcgg tgtattcatg gcagttatca
    27241 tgaacgcaac taaattagct caagatatag aaggaaacgt tgatgtctct gtattcgtag
    27301 atatcggtac aaaagacgaa gagatgaaaa agctagaaaa agaattgaat gacttagatc
    27361 atgttaaaaa agttgaattt tcaagcaaag aacaggaatt gaaaaaaatc caaaacgaaa
    27421 tgggtgattc ctggaaattg tttgaaggtg acaataaccc gttatatgat gtgtatgtag
    27481 taagtgctac tgatccttct tatacaaaac aagtagcaga agaagcagca gatttgaaga
    27541 atgtttctcg ttctgattac ggtggagctt cctcagatcg gattttccaa atcgctggtg
    27601 cagttcggac atggggatta gttgcagcgg cattgctgct gttcgtagcg atgtttctca
    27661 tttcgaacac gattcggatc acgattttgt ctagacagcg tgaaattcaa atcatgcgtt
    27721 tagtcggtgc aaaaaatgga tatattcgct ggccgttttt ccttgaaggt ggctggatcg
    27781 gtctgatcgg tgcgattctt ccgattttga tcatcacatt tggttatgca tggtttttcc
    27841 aattgatcaa tccatcatta ttacgttccc attattcatt gattcatcca caaaatatcg
    27901 tttggaaaat caatctttta atggtcggca ttggtgtgat cattggttca cttggctcga
    27961 tcatttctat gagacgtttc ttgaaattct aataccacaa tataaataaa atgagaaagt
    28021 tggacagttt cgttccagct ttcttttttt attggctagg agaatagaag gaagcggctt
    28081 ttttcccttt actttaaaaa acaacttggg tattcttaag ttggtagaag aagaggtgaa
    28141 gccgtgaaaa ttatcgttga caattttttt acgatccttt tgattatgaa tattttgctt
    28201 tcctttatca tcgtttttcg agaaagaaag gaaactgctc aaacatgggc atgggtgctc
    28261 gttttgatgt tcattccagt tgtaggattt attttatata tttttttagg aagagggatt
    28321 tccaaagaga aaatctttga tttgaagatc caagggaaaa tcggtaagaa cctggaaatt
    28381 gaagaagaca aacaggcatt gatgcgaggg ctttatcctc atccgcctac tggacaagta
    28441 gatgtgaaac aattgatcta tatgctcacg gtatttgaaa gtacgctcta tacgactaaa
    28501 aatgaaatca ctttatttac agatggtcgt gaaaaattcg atgcgctgat tgaagatatc
    28561 aagcaggcag aagaccatat ccacttccag tattatattt atcgcagtga tgcattaggt
    28621 gaagaagttc gagatgcatt gacagaggcg gcaagacgag gggtaaaagt cagagtcctt
    28681 cttgatgcat ggggttccac acaagtttct tccagctttt ttcagaattt gaaaaaagca
    28741 ggcggagaag tcgcattttt ctttcctcta tttgttccgt atattaatcc tcggatcaat
    28801 tatcgcaatc atcgtaaaat cgtagttatt gatgggaaaa tcggatatac aggtgggttc
    28861 aatgttggaa atgaatatct aggattggta aagaaatttg gttattggcg agacaatcat
    28921 ttgcgtattt acggagaagc ggtgtatacc ttgcaaaacc gttttttgat ggactggaat
    28981 tctcagcata caaaagaagt aggctattct cccaaatttt ttccgtccat tcactctact
    29041 ggtgatatag ctgttcaaat cgtgacaagc ggaccagata ccgaacatga acaaatcaaa
    29101 atgacttatt tgaaaatgat ttcgttagcc aaacgggaga tattgatcca aacgccttat
    29161 tacataccgg atggatcgat tcatgaagca ctgaagttgg ctcttctttc aggcgtaaaa
    29221 gttcatatcc aaatacctaa caaaccagat catcttctcg tttactgggc aacgtattct
    29281 ttcgctgcag agctaatcga gtatggagca aggatcgaaa catatgagaa tggatttatt
    29341 catgcaaaga cgatgattat cgatggtggc attgtttcag ttggctcagc aaatatcgat
    29401 gttcgttcat ttcgcttgga ctttgaggtc aatacactgg tttatgatga acgaatggct
    29461 tcacgtgtaa gaaaagcttt ttttgaagac tcgaaaattt ctacgcatct cacaaaagaa
    29521 atgtatgaaa accgaggaat aatcatcaag atgaaagaag gattagcaag attgatttcg
    29581 ccgctccttt aaaacataaa aaggacatga gaaccatcaa cagatggaga tctcgtgtcc
    29641 ttttctaacg gggccatttc tgagagtacg tcagaaatgg agaaagcgtc tcgcatgagc
    29701 gaaattaact ttggggtatc tcttgttttt tggggaaagc aagaaataag ctaatatatg
    29761 ctattaaaag agacacattt caaagccagt atagcataga aacttttaat gagatagttt
    29821 tttttacaat tatgagaatt tattttgtta tttaatgatt ttgattcgtt ttcatcaagt
    29881 aatgaaaatg ataaaataaa catactttag gattaaagga gatttctaat gaaaaaactt
    29941 aaattattct taccaataat aggattagct attcttttat caggctgttc aaagtggatt
    30001 gatcgaggag aatccattac ggctgttggc tcttctgctt tacagccttt agtagaaaca
    30061 gttgctgaaa cttaccagac agaaaatcct ggaaaattca tcaatgtaca agggggcgga
    30121 agcggaactg gtttgtctca agtacagtca ggagcagtcg acatcggaaa ctctgatttg
    30181 tttgcggaag aaaaatcggg gatcgatgcc tcagcattag tcgatcatcg agtagcagtt
    30241 gttgggatca ctcctattgt gaacaaggat attggcgtaa aagacatcag tatggaaaac
    30301 ctgaaaaaac tatttacagg cgagatcaaa aactggagtg aagtcggtgg aaaagatcag
    30361 ccagtagtta tattgaaccg cgcttcagga agcggaaccc gtagcacgtt tgaaaaatgg
    30421 gtattggacg gagaaacagc tgttcaggca caagaacaag attcaagcgg aatggtgctc
    30481 cagatcgttg ccgacacacc cggtgcaatc agctatgttg ctttttctta tgtttccgat
    30541 gatgtagata cgttgaatgt taacggtgta gaaccgacag atgaaaatgt gacaacgaac
    30601 aaatggacga tttgggcata tgagcatatg tacacaagag atcatccaaa aaaactgaca
    30661 aaagaatttt tagactatat cttatctgat gaagtacaag ataacatcgt tggagaacta
    30721 ggttatatcc ctgtttctca tatgaaagtc gaacgtgatt ggcaaggaaa tatcgttaaa
    30781 gagtaatctt tacacaaaat ttacaaactt agctaaactt ttacatgtta ttaaaaccaa
    30841 attgatgttc aatttgtaca ctgagtgagt atgttaaacc tgattaaggg ggagtcatct
    30901 gtggaagatg tgaaaaaagt attattaaca aaatcaaaac gttcaaaaat ggaacagcgt
    30961 ggaaaattca tcagtttttt atgtatcgcg ctgatcgtgt tggttgtatt atctattttt
    31021 tattttgtag ctagtaaagg attggccacc ttttttactg ataagatcaa tgtatttgat
    31081 tttctgtttg gtacagattg gaatccaagc caaatcggag caaatgggaa accaatggta
    31141 ggggcattgc ctatgatcac aggttcgttt attgtgacct ttttatctgc tatcgttgcc
    31201 acgccatttg ccattggtgc agcagtattc atgacggaga tctctcctaa aaacggaacg
    31261 aagatcttac agccggtcat cgagctgctt gtaggtattc cttctgtcgt ttatggtttt
    31321 atcggtttat cagttatcgt gccttttgtc agaagtattt ttggcgggac tgggtttggt
    31381 atcttagctg gtacatttgt gttgttcgtt atgatcttgc cgactgtcac aacgatgact
    31441 gttgatgcat taaaggcagt gccacggcac tatcgggaag cttcattagc gcttggtgcc
    31501 acaagatggc aaacaatcta caaagtcgtg ctacgagcag ccgtgcccgg gattttaaca
    31561 gcagtcgttt ttggtatggc tcgtgcgttt ggtgaagcct tagctatcca aatggtcatc
    31621 ggtaatgcag cattgatgcc aacaggtctt actactcctg cttctacatt gacgtccatc
    31681 ttaacaatgg gaatcggaaa tacaatcatg ggaacagttg aaaataatgt gttatggtca
    31741 ttagcgctga tcttgctatt gatgtcatta ttctttaata tcgtgatccg tatgattggg
    31801 aagaaaggag agctgaaata atgaacgcaa aacgctcaga taaaattgct acaggtgttt
    31861 tatatatagt ttcggggatc atcgtattga ttttagcagc tttactactt tatatcttag
    31921 ttcggggaat accgcacatt tcatggtcct tcttgaccga accttccaaa gcctaccaag
    31981 aaggcggtgg aatcgggatc cagctattta actcgattta tttgcttttg atcacgatga
    32041 tcatcagttt tcctatttct ttaggtgcgg gtatctatct ttcagaatat gctggaaaaa
    32101 actggctaac agatgtgatt cgcacgtcga ttgaaatctt gagctcgttg ccttctgtcg
    32161 ttgtcggttt attcggtttc ttggttttcg ttattcaatt ccagtatggt ttctcgatta
    32221 tttctggtgc attggctttg actgtattca acttgcctct tttgactaga acagttgagg
    32281 agtctttaca agcggtccat tacacacaac gagaagcagg attggcctta ggtttgtcac
    32341 gctgggaaac agtgataaaa gtggtggttc cagaagcgac accagggatt ttgactggtg
    32401 tgatcctaag ttctggaaga atttttggtg aagcagctgc attgatctat acggctggac
    32461 aaagtgcacc tgcgttagat tttacgaact ggaatccatt aagtgtttca agcccgatca
    32521 gcattttccg tcaagctgaa acattagctg tccatatttg gaagatcaat acagaaggca
    32581 cgatgcctga tggtgtttct gtttcggctg gcgcttctgc cgtgttgatt attgctgttt
    32641 tactgttcaa cttcggcgct cgtgcgattg gcaaacgatt ataccgaaaa atgacttcag
    32701 cctaaggaga agaagcaaaa tgaaaaaata taactggaat gaaacaaatg tcatcaccat
    32761 gccaaaagag aaagaagtag cactatatac ggatgatcta catgtctggt atggcgacaa
    32821 tgaagcaatc aaaggagtgg atctggaatt tgaaaaaaat aagatcactg cactgattgg
    32881 tccttccggc tgcggaaaat ctacgtatct tcgttcgttg aatcgaatga atgatgggat
    32941 cgctaatacg aaaatcactg ggaaaatcat gtatcaaggc gtagatatta atgcgcctca
    33001 agtggatgtt tatgagatgc gcaaacggat tggcatggtt tttcaacgtc ctaatccgtt
    33061 cagcaagtcg atttatgaca atattacatt tgctttgaaa cagcacggcg aaaaaaacaa
    33121 aaagaaatta gatgaaatcg ttgaaacaag cttgaaacaa gctgccttgt gggaccaagt
    33181 aaaagacagc ttggacaaaa gtgccttggc tttatctggc gggcaacagc aacgtttatg
    33241 tattgctcgt gcgatcgcga tgaaacctga tatcttgctg ctagacgagc cggcaagtgc
    33301 tttggaccct atttcgacag gaacggttga agaaacactt gtaaacttga aagaagacta
    33361 tacgatcatc atcgtgacgc ataacatgca acaagcagct cgtatcagcg actatactgc
    33421 ctttttctat ttaggaaaag taatggaata tgacaaaact aacaagatat ttaccagacc
    33481 aaaaattcag gcaaccgaag attatgtgtc cggtcatttt ggttaagacg gaggcctcaa
    33541 catgacaaaa gagattattt catctaaaga tcttcatctg tattatggaa agaaagaagc
    33601 actaaaaggg atcgatctaa gcttcaatca aggggaaatc actgcgatga tcggtccttc
    33661 tggttgcgga aaatccactt atctgcgttc tttgaaccgg atgaatgacc tgattccgaa
    33721 tgtaacgatc accggaagtg ttttatataa agacaaagac atctatggtc caaaaacaga
    33781 tgttgttgaa ctaagaaaag aaatcggcat ggtattccag cagccaaacc catttccttt
    33841 ttctatctat gaaaatgtca tctatggatt gaaattaaaa ggggaaaaag ataaaaaagt
    33901 ccttgatcaa gtagttgaag aaagcctgaa ggcagcgtct gtttgggatg atgtgaaaga
    33961 caaattgcat aaaagtgctt tatctttatc cggtgggcag cagcagcgtg tatgtatcgc
    34021 acgtgttttg gcagttaatc cggaaatcat cttattggat gaaccaacaa gtgcgcttga
    34081 tcctgtttct actgggaaaa tcgaaagtat gctgttagaa ttaagagagc ggtatacgat
    34141 gatcatcgtg acccataata tgtctcaagc ttcacgtatt tcagataaga cggcattctt
    34201 tttagatggt cacttgatcg aatacaatga tacgaagaag atattcatga atccagaaaa
    34261 acaagagaca gaagattata tttcaggtcg atttggataa ggaggaaacg gaatgctacg
    34321 tacacaattt gaagaagaat tgctgaatct ccataatcag ttctacgaaa tggggatgat
    34381 ggtgagcagc gccgtgcaca agtcagttcg tgcatacatc aaacacgata aaatcttagc
    34441 ccaagaagtt atcgaaaacg atataaatat caataacatg gaaacacgct tggagaagaa
    34501 aagttttgaa atgatcgcat tgcaacagcc agttacaact gatttgcgta tgatcattac
    34561 ggtcatgaaa gctagctcgg atttagaacg tatggccgat catgcggtgt ctgttgctaa
    34621 atccacgatt cgcgtaaaag ggcagacgag aataccggaa atcgaaaaag agatctccga
    34681 tatgtccgat tatgtgaaaa aaatggtgga caacgtccta gttgcttatg tcaaaacaga
    34741 cgaaaaagac gcacgtacga tcgccaatat ggatcaaaga gtcaatgact atttcaatcg
    34801 aatctataat gcaacaatca aaaatatgca ggaaaatcct gagacagtca tcagtggaac
    34861 agactacttg aatgttgcca gttatctaga acggatcgga gactatgtga caaacatctg
    34921 tgaatggatc gtctacttgg ctacagggaa aatcactgag ttaaatacga accacaatga
    34981 gattttctaa actgaagctg tgattatgcc gtttgtttag gaaaagaggt tgtgaaatca
    35041 gcggttgtga aaaagctgtt ctgcatcaag taataagaac agtcaaacaa aaaatagctc
    35101 ctcatatttt tcgtttggct ggacttatta ccgcagatgc aagcttttga acaccgttta
    35161 ttcgggaaag ggtttgtgac aaaacatgtc acaaaccctt ttcatcattt catagaaagg
    35221 ataaagaatg caaaaaaaaa ttagcatcat cactatattt tttatttgtg tgcttacttc
    35281 atgggttatt cagcatcaag ccgctttata tgaagtacct gtcatcgaga tagaaaaagt
    35341 acgaataaaa gaggatcatc aattgattac aggaaaactg atgaacacgg atcaaaaagg
    35401 agaatcgatt tatttatcgg ttccttatca aaaaaatcaa atagaaacag caaatataaa
    35461 agcgagacaa atgtatctag tccaaatgca tggagatgaa tggcagctag tagcaaaaaa
    35521 aaatgatggc tggcttttct tcttcacaag tatcttcatt ggcgccatgt tgcttattgg
    35581 tggaaaacag ggtctatttg ctttaggaag tctcatcatc aatatcgttt tacttttact
    35641 tttgttgctc atctttacta aaacagaagg gatttctcta ttaaatatat ctatcctgtt
    35701 cgtcgttata gggatgatta ttagcatatt ggctttagat ggatggaata gaagcagtat
    35761 aatcaaaatt tcagctgcag ttacgagtgt ttttttagca ttttttattt gtcttttgac
    35821 aatgaatcat tggaaagacc aaggactgcg ctatgaggaa ctgaattatc tgacacgacc
    35881 gtatcgttct gtttttcttt ctagcgtact gattggaaca gctggaggaa cactcgatac
    35941 cgttatcaca gtcattgcga cattagaaga attgactaag cgacaaccag aaataacagc
    36001 gaaacagtta tggctttcag gaaggcaagt gggtcgagat gtgattggct cgatgaccaa
    36061 tatccttttg ttcgtctata tcagtgggtc agttccatcg cttcttttat atttaggaaa
    36121 tcaatggtcg ttcaaagaaa cgatagaaca gcatttatca ttggaatttt tacgcgtcat
    36181 tgctggcagc ttaggaatag tattatcgat ccctatctcg gtcctctttt ttctgactgt
    36241 aaggaggctg aaacgatgaa tgtacttatt atacttgttg ggattttagc aatcagcgtg
    36301 ctacttattg gcggtacgca aggtatgtat attcttttag gcttattcat caatttaggg
    36361 atctttttcc tgttgttatt cggttatcat cagaagtggc cgatactggc attgtcggtc
    36421 ataggatttt tattgattgc tgtggttatc ttatttttta tcaacggtta taatctaaaa
    36481 atgcgtgcag cttttgtgag tatactgatc tttttatttt gtttcctact attgcttcct
    36541 atcactgatt ttttagcaat ccaaggtttt acatcggtag aactagaaga attatctggc
    36601 ttagataaga ctttagcgat tgattttaga ttacttacga gatcgttgct tctgattagt
    36661 cttagtggtg ctgttttaga tgcgagtgta gcaatttcta gtgggacatt cgaagtatat
    36721 caggcaaatc cgcaattatc tttttaccaa ttgcgccatt ctagtttcgc tattgctaaa
    36781 aaaatattgg cgtcaacagt aatgacactt ctttttgcat tcatgggaag ttctttagcg
    36841 ttgatcctct ggtttattga tttagacatt ccttttcagc aagtgatcaa tgaaaaatca
    36901 tttgttctag aatatacgat ggctattctt accacattgt ccgcactact tgttcttcct
    36961 ttaacctgcg tgactacagc atatctcttt acaaaaaaga aagaacaact caagatggag
    37021 taaaagttac aacttaagtc ctatgacatt tttactgaat cagtgcataa tatggatgta
    37081 aggaaaagct tgcaaacaat tttgcttaag gaggcaattt ccatgaaaga aagagaacgt
    37141 attttagatt tagtgaaaaa aggaatcctt tcgactgaag aaggcttaga tttattagaa
    37201 agcatggcaa ctgaaaaaga cgaaaagcaa atcaaaaaag aagcagacaa ggtaactgct
    37261 agtcataaag aaaaagatca ggcaagtcaa ttgatcgata aacttgaaaa cggcgaagaa
    37321 gaaatttctg agccagttga tccaaaggaa agagaaagac aagatcaaga aaacttagaa
    37381 aagatcttag atgaactagc aactgaagcg aataaaactt cggcgcgtct agatgaagta
    37441 aatgctcaat tcgctgacaa taaggcagca cgcaatgaga aacaggaagc attgatgcag
    37501 ctgaatacaa aagaagaatt aggcgagttg acagaagaag aattagcaca gcgtcagacg
    37561 ttagaagcag aaatcaaaga attagaagct agtggcgata cactgttaga agaacaattg
    37621 aaattagaag cagaattaaa agatatccgt aagaatcaat ggtctgagaa aaaagaaacg
    37681 tttactgata agtttgaatt gccagatgat tggaaagatc aagcaacaga tacactgaat
    37741 caagtaggcg aaaaaatgtc agaagctgga tcacagttag gcaaattctt gaagaaaaca
    37801 ttccaaactg tttcagaaac agtgaatgac aacatggaat ggaaagatgt cagcttacgt
    37861 gtccctggga ttgcgacaac gaaatttgag catgaatttt actatgaagc tcctgcagca
    37921 agtatccttg atatcaaggc agcaaacgga aacgtgacgt taaaaacgtg ggacagcaac
    37981 gatgtgaaag ttgaagctaa gatcaaactt tacggaaaaa tgggtgctga accatttgaa
    38041 gcattctctg aacggagcca gattgaggtt aatgaagatc atatctcctt ccaaattcca
    38101 aataaacgtg tgcgtgcaga ccttgttttc tacttaccta aacgagtata cgaccacgca
    38161 gctattaaac ttctaaatgg caacatcatg attgaaacat tggaagctaa agatatttat
    38221 acaaaatcta cgaatggtaa catcatcgtg gaccaattaa ctgcaacgat gctggaagta
    38281 gaaggtgtaa acggcaatat cgatattcga aacgggaaca tcttagattc tattatcgaa
    38341 acagtaaatg gtacagtgac atttggtgcg acagcagaaa acctttctgt gtctttggta
    38401 aatggtgatg tacgtcttac gattaaagaa gataatttga aaaaagtaga agcaagttca
    38461 gtgaacggaa atgtcaaagt cgctttacca gatggcatcg gtttggaagg acatgctaaa
    38521 acaagtcttg gaagtatcaa cagccgtctg tccaattacg aagtagttcg tgagaaaaaa
    38581 gaacggacaa accaaatgtt gcaattccgt cgtgtttcag atggtgaaat cgcgcaagtg
    38641 caactttcaa cgacaacagg aagtatctat ttaaaagata cagataaata aagaggttgt
    38701 gaaagagatg ttcagccttg agcaataaga agcgattttg taaaatagct tcccctattt
    38761 tacaaaattg tgccttattg ccgatagtct atctcttgaa caccgtttac caagagatta
    38821 tgaacctgcc aattatcgcc gagtcacaag gaataatttc agaaaaagct cgtcacgttt
    38881 tatgaatatg gtaatttgtg accgagaatt ttgcagaaag acaccgatta agaagaaggt
    38941 tgtcacaacg attttcttca tttaaaatag tagaacagag gtgagacatg atgaaaaaaa
    39001 agttgacaaa atctaataaa aatgttgttt tgacagggac tttagcagga ataggagaat
    39061 acatcgggat tgatccgact gttatccgtg tgctttatgt attcctgagc ttagttgctt
    39121 ttggaagtcc aattatttta tacattttat tagctttgat tatcccatct gcgcgttctg
    39181 cacgaacaaa ctatggacat aacaatgcat attatgaaaa aaacaattat cgtgaacaaa
    39241 ctcgtgaaca aacaaaaaaa cgcaaagaag cggaaaaaat cgatgatgac gaatggagtg
    39301 acttttaatg tcgtactttc aacgtatttt agttaatacg ttaacattca tatcattggc
    39361 tgttcttttt cctgcgatgg tccatgtgag cagtatttgg acggccgttc ttgccagctt
    39421 cgtattgtct gtgttgaata tgtttgtcaa accgattttg acgatactgt ccctgccctt
    39481 tacattagtc acattgggat tgtttacgtt tgttatcaat gcagcgattc ttaaaatgac
    39541 ctcctttttt gtgggagcag aacattttag tttttcttca ttcggtgcag cgttgttagt
    39601 agctatcttg atgtcgcttg tcaatgtaat tgtcagcgaa cataacagta gaaaataaaa
    39661 gaaagctgtg ccgaactatc ggcgcagttt ttttatttag aatcaggaat gcgttttcta
    39721 ttacattggt aattaaataa aaacgttttt tttaattggt agtacttttg gctagacaat
    39781 gataaaatga tagagaacac tctaatagaa atgaggtagc atgaatgtca gaagttgtaa
    39841 aagttagcca attagtgaaa aaattatctt tggaaatcgt gacaggcgac gagaaaagct
    39901 tagaccgtgt catcgtaact ggggacatct cacgcccagg actagagttg actggttatt
    39961 ttaattatta ttcccataat cgtttacagc tatttggcag taaagaaatc agttttgcgg
    40021 aacggatgat cccagaagag cgtttgatca tcatgagaag attatgtagt gaagatatgc
    40081 ccgcatttat tatttcaaga ggattaccag ctccagaaga aatgatccag gcagcaaatg
    40141 aaaatgggat agctgtactg cgttctccca tctcaacttc tcgtttatta ggagaattat
    40201 ccagttattt agatggtcgc cttgcccctc gaacaagtgt ccacggtgtt ttagtggatg
    40261 tttacggatt aggtgtactg attcaaggtg acagtgggat aggaaaaagt gaaacagcgt
    40321 tggagttgat caaacgcggc catcgattga tcgcagacga tcgagtagat gtttatcagc
    40381 aagatgaatt aacggtgatc ggagaaccgc ctaaaatcct ggaacatctg attgaaatcc
    40441 gaggaatcgg gatcattgat gtaatgaatc tttttggtgc gagtgcggta cgtggctcta
    40501 tgcaagtcca gttggctgtt tatctggaag catgggcaaa agataagaaa tacgaccgtc
    40561 tgggctctga agacacgacc gttgaaatcg cggaagtggg cattccacaa ataaaaatcc
    40621 ctgtcaaaac aggacgaaac gttgcaatca tcattgaagt agccgcgatg aatttccgtg
    40681 cacgtacaat gggctttgac gcaacgaaaa catttgaaga aagattaagt cgcttgattg
    40741 aagaaaactc aggcaacgac tagcaaggag tgacaatatg ttagcacaaa tcaatcgagt
    40801 tgcttttgag cttgtaggga ttcctatcta ctggtatgca ttgatcatcg tatcaggtat
    40861 cattatcgct atgtggctta gcagcagaga ggctgttcgg gtaggattaa aagcagatga
    40921 tgtgacagat tttatgttgt ggggattgcc tatctccatt attggcgctc gcttatatta
    40981 tgttttattt gatcttcccc agtatatagc tgatccaatc caaatattca atatccgttc
    41041 aggaggctta gctatttatg gcggactgat tgcaggcgcg atcacgctgt atttttttac
    41101 taaatatcat tttatttctt tgtggacttt tttagatatc gctgcaccaa gtgtattatt
    41161 agcacaggca atcggtcgtt ggggaaattt catgaaccac gaagcatatg gaccagacac
    41221 gacgcgaagc ttcttagaaa gtctgcattt accgcaattc atcattgata atatgtatat
    41281 cgaagggatc tatcgtcaac caacatttct ttatgaatca ctttggagtt tgattggatt
    41341 tattttgctt ttgtttttac gaaagaaacc acaccttttg aaaaaaggcg aagtcctttt
    41401 atgttatcta atgtggtata gtttcgggcg tttctttatt gaaggattac gtacagacag
    41461 tctttacttt ttaggtgtga tccgtatttc tcaattatta tctgctgtgt tatttgtcgg
    41521 tacattgatt ctatttattt ggcgcagaaa aaaaggaaat ttaccggatt atgaccgctc
    41581 attaggaaaa aatcaacaaa ttatctgatt tttagagcgt taaaaatgaa aaaaatttca
    41641 ggagtggtaa aatgaattat agagtagcgg ttttaggacc aggctcttgg ggtactgctt
    41701 tggcacaagt cctcgccgaa aacggacatg acgttcggat ttggggacat cgtgcttcac
    41761 aagtagaaga aatcaacacc caacacacga atcaacgtta tttaccagat cattatctac
    41821 ccgattccat ccaagcattc accaatatgg aagatgcagt aaaagacgca gatgcggtgc
    41881 tattcgtcat tccaacgaaa gcaatacgtt ctgttgcaaa agaactagtg cctaaattga
    41941 atacaaagcc agtgatcatc catgcaagta aaggattaga acagggaaca cataaaagga
    42001 tctctgacat cttgttagag gaaatcccag cagaaaaaag aaaagctgtt gttgttcttt
    42061 ctggaccaag ccatgcagaa gaagtagcaa aacatgacat cactacgatc acagctgcaa
    42121 gtacagatgc aaaagcagca aaatatgtac aagagctgtt catgaataat tattttcgga
    42181 tctataccaa tcctgatgtg atcggtgtag agatgggggc agctttgaaa aatattattg
    42241 ctattggtgc tggcgctatt cacggcttag ggtttggcga taatgcaaag gcagcaatca
    42301 tgacgcgcgg attagctgag atcagccgtt taggtgtagc aatgggtgcc aacccgctga
    42361 cctttatcgg attaagcgga ataggcgatc tagtcgttac ttgcacaagt gttcattcta
    42421 ggaactggcg cgcaggcaac ttgttaggac aagggcagcc cctagaagag gtgctagcca
    42481 atatgggaat ggtcgttgaa ggtgtgaata ctacccaagc agcgatggaa ctagcccaaa
    42541 tgatggaaat cgaaatgccc atcactcaaa caatctatga agtgctgtat gaacaaaaag
    42601 aaatcaaaac agccgcaaaa gaaatcatgt tacgtgatgg aaaaaaagaa aatgaattct
    42661 cattaacaat tttttgaagg agcgaatgta tatgaaagtt aaaaaagcag ttattccagc
    42721 agcaggatta ggaacaagat ttttaccagc aacaaaagct atggcaaagg aaatgttgcc
    42781 aattgttgat aaaccaacaa tccagttcat cgttgaagaa gcgttgaaat caggtatcga
    42841 agacatcttg atcgttacag gaaaagctaa acgaccaatc gaagatcact ttgattcaaa
    42901 tattgaatta gaaacaaacc taagtgaaaa aggaaagacc gaattattga aattagtaga
    42961 agaaacgaca gatgtcaact tgcatttcat ccgtcaatct catccaaagg gtttaggaca
    43021 tgccgttctt caagcaagag cctttattgg aaatgaacca tttgtcgtta tgctaggcga
    43081 tgatataatg gaagacgaag taccattgac acagcaatta atcgatgatt acgaacgtac
    43141 tcatgcttct acgatcgccg ttatgcaagt tcctcatgat gaaacttcaa aatacggaat
    43201 tatcaatcca ggagaagtat tggaagatgg gttatacaat gtcaaaaact ttgttgaaaa
    43261 accagatcca agcaaagcac caagtgattt agctattatt ggacgttact tattgactcc
    43321 agaaattttt gatgtattgt ccgagcaaca accaggagcc ggaaatgaaa tccaactaac
    43381 tgacgcaatc gatacattga ataaaacaca acgcgtattt gcccgtaaat tcacaggtaa
    43441 acgttacgat gtcggtgaca aatttggttt tatgaaaaca agtattgaat atggtctaac
    43501 acatccagaa gtaggtgcac ctttacgtga gtacatcctg tctttaggta aaacattaga
    43561 agcagaagga agcaagatcg atcataatcc aaaaggaata acagaaatca aagaagaaaa
    43621 gaaaaaataa cccttttttc taacgagctt cagctgctaa ttgaataaaa agtagttgaa
    43681 gctcgttttt atttgtttaa aaaacacgaa aatgctatcc ttatagaaaa gagagacaag
    43741 aagggagttt gatcatgtca ggaggagaaa ttgcaggatt gatcgcagca atcgcctttg
    43801 cgatacttgt cgtttttatc gttcgtgtgt tgttgcaagt ttctaaaact ttagaaaaag
    43861 tagatcagac tgtagcagaa gcaaatacga cgatcgaaat cgtcacaaaa gacgtagatc
    43921 ttttgtctag acaagtagaa ggcttactgg tcaaaagtaa tgaactgctc ttggatatca
    43981 atggaaaagt cgcaacgatc gatccattgt tcacagcagt tgctgattta agtgaaagtg
    44041 tatcagaact taatcattct agtaaaaaca tcgcgacgaa agtcggcgga atcggtaaaa
    44101 cgacagctaa agcgacggtt gccggaaaag tcggtgggac agcgatgaaa ttttttaaaa
    44161 acaaaaaaca tactgaaacg acaggaggaa aataaaaatg gctaaaaaag gtggattttt
    44221 attaggtgca gtagtcggag gcacagccgc agcaatcgcc gcgttattgt tagcaccaaa
    44281 atctggtaaa gaacttcggg atgaattagc tgcccaagcg gatgatttga tggatatggc
    44341 ttcagattac acagatatgg cgaaagaaaa atcgactgag ttaacaggta ttgcaaaaga
    44401 aaaagcgcaa gatctttctc aacaagcagg tgatctagca ggtagtgtca aacaaaaagt
    44461 aagtgaatca gcagatgaag caggaagtga atctgacgat atcttgttaa atcttaaaga
    44521 acaatcagct gacttatcta atcgtttcaa aaaaacggca gaagatatga aaaaacctgc
    44581 agatgaatta ggaagtattg ctaaagatac ttcagaagat atcatgattg atgtaaaaga
    44641 cgctgctggt aaagcaggag atactgtcaa tgaaggaatg tcagaagcaa aaccagttgc
    44701 agatgactca aaagaaatga tgaaaatggc aaaagaagat gcgaaagaag ctaaaaaaga
    44761 aataaaagat acaatgacag attcatctac aaatcactaa aatcacaaat ttatagaata
    44821 aa
//
LOCUS       DAZKKW010000016        39598 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.016, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000016 DAZKKW010000000
VERSION     DAZKKW010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 39598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39598
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.016"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(194..1960)
                     /locus_tag="ACG8PO_000844"
     CDS             complement(194..1960)
                     /locus_tag="ACG8PO_000844"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0206192.1"
                     /translation="MQMIGGLTMKSFYKNKFALSDQGAEALTKASISSFFVYCINMVP
                     AFIIMMLIDELVLENTKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLRVQ
                     IADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVMLL
                     VGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEINS
                     FNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLLTG
                     EVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSPLK
                     SFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDYDE
                     GCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAARL
                     ANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLDAE
                     NEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTHNELYRRSEIY
                     KNLIKKTKLSEKFVYEKEAQSR"
     gene            complement(1920..3674)
                     /locus_tag="ACG8PO_000845"
     CDS             complement(1920..3674)
                     /locus_tag="ACG8PO_000845"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0206193.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLQ
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQHLIKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHQVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            complement(3784..4353)
                     /locus_tag="ACG8PO_000846"
     CDS             complement(3784..4353)
                     /locus_tag="ACG8PO_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HFQ0206194.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVASWALMQLGSAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
     gene            complement(4371..5786)
                     /locus_tag="ACG8PO_000847"
     CDS             complement(4371..5786)
                     /locus_tag="ACG8PO_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0206195.1"
                     /translation="MFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSGKST
                     LLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTNTTS
                     ELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLSLNS
                     KLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELIDQVLFVKNRT
                     VKTTTIQLLKTENNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILKDINFDV
                     KVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDYQFFTE
                     SVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSIASLSKTNL
                     FLFDEPTSGLDYINMERIADLIKDLKEKGAVVIATHDPEFLYKTCNRIIYLKNGGIKK
                     DIKLRVEDSTLIEQIFNDITK"
     gene            complement(5797..6465)
                     /locus_tag="ACG8PO_000848"
     CDS             complement(5797..6465)
                     /locus_tag="ACG8PO_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HFQ0206196.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFATSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            complement(6595..7179)
                     /locus_tag="ACG8PO_000849"
     CDS             complement(6595..7179)
                     /locus_tag="ACG8PO_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0206197.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLI
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            complement(7510..7743)
                     /locus_tag="ACG8PO_000850"
     CDS             complement(7510..7743)
                     /locus_tag="ACG8PO_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HFQ0206198.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            complement(7758..8708)
                     /locus_tag="ACG8PO_000851"
     CDS             complement(7758..8708)
                     /locus_tag="ACG8PO_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding
                     lipoprotein"
                     /protein_id="HFQ0206199.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSINSKDKDKVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            complement(8705..9547)
                     /locus_tag="ACG8PO_000852"
     CDS             complement(8705..9547)
                     /locus_tag="ACG8PO_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease subunit"
                     /protein_id="HFQ0206200.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVIVVLFFKELLIT
                     SFDPLMAKAIGMNVSFYHYLLMVLLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGASSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERSL"
     gene            complement(9549..10268)
                     /locus_tag="ACG8PO_000853"
     CDS             complement(9549..10268)
                     /locus_tag="ACG8PO_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFQ0206201.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKKLKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIGEGE"
     gene            10816..11118
                     /locus_tag="ACG8PO_000854"
     CDS             10816..11118
                     /locus_tag="ACG8PO_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003013637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0206202.1"
                     /translation="MTIAANNDTSVRLAVNWGEVKFLCAYPKQFKNFITTILGDDFIE
                     SRVYLNKKQTCAFLGIDNNTLDKWIRIYNFPVIRVGGVVRFDVEDIKDFMNTFKIT"
     gene            11190..12416
                     /locus_tag="ACG8PO_000855"
     CDS             11190..12416
                     /locus_tag="ACG8PO_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0206203.1"
                     /translation="MPTKLSNGKYKTNLRYPKRFKEITGIASEKYQKTFPNRQLAIKA
                     ENDMKKKIEKVLREENANSLELKGKITFKKFYESKWLPRYELGQTIRSNRPPSDITIS
                     NTKDIFRLHILPMFGEYAMNYLNINTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILNYIEFNRTTKIIQSITAPKKNALEEKRIQEGKQALSSKELTNWIEAVNDDFNNHLL
                     TFHDYTLFMITLYLGDRKSETYALQWKYIDFEKQTVRLKHTLDKYQRKKFTKGRKDTV
                     IQVPEVVMTLLSEWKSVQADQLLKLKIKQTLDQYLFTYTKPSGEVNCPVHADYLNYRI
                     NSIKRRHPDLVHLSPHKLRHTYATIARQGGADMNQISNALTHSDISTTKIYVNTPDIV
                     DKAVFEAFQRGLNKCD"
     gene            complement(12504..12896)
                     /gene="rpsI"
                     /locus_tag="ACG8PO_000856"
     CDS             complement(12504..12896)
                     /gene="rpsI"
                     /locus_tag="ACG8PO_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFQ0206204.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(12912..13340)
                     /gene="rplM"
                     /locus_tag="ACG8PO_000857"
     CDS             complement(12912..13340)
                     /gene="rplM"
                     /locus_tag="ACG8PO_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFQ0206205.1"
                     /translation="MAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPTFTPHL
                     DTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLIETSVK
                     GMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            complement(13874..14341)
                     /locus_tag="ACG8PO_000858"
     CDS             complement(13874..14341)
                     /locus_tag="ACG8PO_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HFQ0206206.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(14461..15999)
                     /locus_tag="ACG8PO_000859"
     CDS             complement(14461..15999)
                     /locus_tag="ACG8PO_000859"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0046177 - D-gluconate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFQ0206207.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            16079..16765
                     /locus_tag="ACG8PO_000860"
     CDS             16079..16765
                     /locus_tag="ACG8PO_000860"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HFQ0206208.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(16966..17493)
                     /gene="msrA"
                     /locus_tag="ACG8PO_000861"
     CDS             complement(16966..17493)
                     /gene="msrA"
                     /locus_tag="ACG8PO_000861"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFQ0206209.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(17577..18254)
                     /gene="pgmB"
                     /locus_tag="ACG8PO_000862"
     CDS             complement(17577..18254)
                     /gene="pgmB"
                     /locus_tag="ACG8PO_000862"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFQ0206210.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(18251..19381)
                     /locus_tag="ACG8PO_000863"
     CDS             complement(18251..19381)
                     /locus_tag="ACG8PO_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFQ0206211.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            complement(19381..21564)
                     /locus_tag="ACG8PO_000864"
     CDS             complement(19381..21564)
                     /locus_tag="ACG8PO_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HFQ0206212.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(21561..22586)
                     /locus_tag="ACG8PO_000865"
     CDS             complement(21561..22586)
                     /locus_tag="ACG8PO_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFQ0206213.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(22631..23458)
                     /locus_tag="ACG8PO_000866"
     CDS             complement(22631..23458)
                     /locus_tag="ACG8PO_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFQ0206214.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(23455..24522)
                     /locus_tag="ACG8PO_000867"
     CDS             complement(23455..24522)
                     /locus_tag="ACG8PO_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HFQ0206215.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKYGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLRGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(24548..25384)
                     /locus_tag="ACG8PO_000868"
     CDS             complement(24548..25384)
                     /locus_tag="ACG8PO_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0206216.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(25381..26250)
                     /locus_tag="ACG8PO_000869"
     CDS             complement(25381..26250)
                     /locus_tag="ACG8PO_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0206217.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(26320..27603)
                     /locus_tag="ACG8PO_000870"
     CDS             complement(26320..27603)
                     /locus_tag="ACG8PO_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0206218.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(27617..29302)
                     /locus_tag="ACG8PO_000871"
     CDS             complement(27617..29302)
                     /locus_tag="ACG8PO_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFQ0206219.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGARVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            29577..30602
                     /locus_tag="ACG8PO_000872"
     CDS             29577..30602
                     /locus_tag="ACG8PO_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0206220.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            complement(31197..34850)
                     /gene="rpoC"
                     /locus_tag="ACG8PO_000873"
     CDS             complement(31197..34850)
                     /gene="rpoC"
                     /locus_tag="ACG8PO_000873"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFQ0206221.1"
                     /translation="MIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYRTLKPERE
                     GLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHIELAAPVS
                     HIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTEREYREKRE
                     QYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLDILEAFRA
                     SGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLKRLLDLNA
                     PGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKNAKRKIER
                     QEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVCEAYNADF
                     DGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTMEEEG
                     REGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMITTVGKII
                     FNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKKKNLGNII
                     AEVFKRFKVKETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIENAHKQVET
                     ITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSGARGNISN
                     FTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTRKEVRHPE
                     TNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNLATGSDVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNPKGQAVIT
                     EVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGAPLTEGSI
                     DPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRVMDPGDTD
                     ILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAASFQETTRV
                     LTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVYSISDIEA
                     QMAAEDAMNNQTK"
     gene            complement(34929..38546)
                     /gene="rpoB"
                     /locus_tag="ACG8PO_000874"
     CDS             complement(34929..38546)
                     /gene="rpoB"
                     /locus_tag="ACG8PO_000874"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFQ0206222.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLFALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            39274..>39598
                     /locus_tag="ACG8PO_000875"
     CDS             39274..>39598
                     /locus_tag="ACG8PO_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HFQ0206223.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQS"
BASE COUNT        11752 a         8094 c         6721 g        13031 t
ORIGIN      
        1 aactggttta tcgccaattt tttaaaactt tgattattcc ttatattata agaatagaca
       61 ttaagtgaac taattcaatc atactgattc tttcgtgtat tcaaaattag atgctgttta
      121 ataaaattat tctttttgaa aaagaattat aactatatcc caaaaattgt aggttaattt
      181 aaagcctatt tcatcatcgt gattgagctt ctttttcata gacgaatttc tcagatagtt
      241 tagttttctt tatgagattt ttgtatattt ctgaccttcg atacaactca ttatgagtac
      301 cttgatcaac tagttttcct tgatccatca ctacaatttt atttaccttt tcaatagact
      361 tcaagcgatg cgatataatt agtactgtct tatcttgtat taaacgattt aaactttcct
      421 gaatacgttt ttcattttcc gcatctaaac tggcagtaat ttcatctaaa attaaaattg
      481 gcgcattttt caaaaaagcc cgtgctatgg ataggcgttg tcgctctcct ccagaaagtg
      541 aacttccgtt ttctccgacc aatgtatgat aaccgtcagg tagtttttca ataaagtctt
      601 cgcagttagc taaacgagct gcttttttca cttcctcatc tgacgcttga ctatttccaa
      661 tacgtatatt ttccaaaata cttgtattaa acaaagttac ctcttgaaaa acaatagcga
      721 ttttgctaaa caaagaagct ggagatacac gtttaatatc atacccatca attaaaatac
      781 agccttcatc ataatcatac agtctagaca ctaatttcaa gatactagtt ttcccggaac
      841 cactagcacc tactaacgca gtcacttctc cttgtttagc cgtaaaagag atatgatcta
      901 aaattggggt attgttatca tatgagaaag agacatctcg caactcaatg tcaaaagatt
      961 tcagaggact atctgatccc tcttggatac tggtttcctt catagctcta attcgctgta
     1021 ttttaggagc taaataaaat atttcaagca ctgcttcttt catagaatcg aaggaatcct
     1081 ttatttttat tgcggctagt aagtaaccaa cgacgtagag tatagtcact tcacccgtta
     1141 acagtaagtg aacacctact agaatgacta ctgctaaact aacatatgag aaaatagagg
     1201 ataaggccat gactgaaaaa gtacttaatt ctacctttaa atgaatcctt tcactctctt
     1261 ccattttttt gaaaagtctg tcttgaactt ttttagatag attaaagcta ttaatctctt
     1321 gctgcaattc tatagtttct tgaaattctt ccgagttttc tctcaaagta tcgtaatatt
     1381 tagtattggc tttcgtttgg gatttctttg ataacaagat taagacaaaa ctaaataacg
     1441 ttggcaaaat aacagctaat cccattttga cattaccaac caaaagcatc actgaaataa
     1501 aagggaaaaa cagagccata ccgccggctt taggtattgc atgactcatc gcatgctcaa
     1561 taccttcaac gtcagacatg atagtttgag ataaatctga taaattatgt tttgaaaaat
     1621 aggataatgg taaatttgac aaatcgtctg caatttgcac tcttaaatgc gcactttctt
     1681 tataagtact attatataag ttttcatact ccctatctaa caaccaatac atgaaaagca
     1741 aagtaacaaa cgatacggca aaataaagcc aacggggctt tgtattttct aaaactaatt
     1801 cgtctatcag catcattata atgaatgctg gtaccatatt aatgcagtat acaaaaaaac
     1861 tagaaataga agctttagtt aaagcttctg cgccctgatc acttaaagca aatttgtttt
     1921 tataaaaact tttcattgtc aagcctccaa tcatttgcat tctcataatt agtgaccaat
     1981 tgtttataca cttgatgttt agccattaat tcattatgat ttccacgttc tactattttc
     2041 ccttctgaca agacaagaat ttcatctaca gatttaatgc tggataatcg atgagctatc
     2101 ataataactg ttttttcttt tatcagatgt tgaaaagcct tttgtagttc atactcatta
     2161 tctggatcaa tagatgcact ggcttcatcc ataataacaa tctttgcatc ttttaaaatt
     2221 gctctggcaa tagctatacg ttgtttttct cctcctgaca gataaactcc tttactacca
     2281 attaaggtat gctctctttc gggaaatttt tctaaaatcg attcgcaacc ggctaatctt
     2341 agcgcgtcaa atactttact tgctggcgct tgaggatttg ctatagaaac gttatcgtaa
     2401 atactctgat tgaatagttt cgtatcttga aagacaaaag caattgtgtt aataattgtg
     2461 tcttttgaat agtgatttaa aggcttacca ccaattttaa tctgtccctt atccacattg
     2521 tagaaacctg aaattagttt agctatcgta ctctttccgc ttcctgattt tcctactaat
     2581 gcataaattc ttttttgttc taaaactaag ttgaaatttt ctaaaacaca ctcttctttg
     2641 tatgaaaagc tgacattttc aaatgcaatg tcacaatttt taatctgtgt ctcgttccca
     2701 tattttagtt ggtcttgctt catttctaaa taaagggagt ctagtgtttc gactgcatat
     2761 cttgcttcaa acaagtgcat acttacatac attattctca tgaaagaaac aaaaataatt
     2821 ccacttaaaa aaaacaacat caacaagtca acagctaata tcttgggagt ggtaagagaa
     2881 gttataaaaa aggttatagg aactatcaaa atgcaaacta tgccaaaaaa tagccattga
     2941 aaaagaacaa aaggtgtttt cccactcaaa gaatacttat atgcaaactt agcataatcg
     3001 ttaattgctt tattcaaggc tttaaatgat gttactttag caccaaatat ctttataact
     3061 tgaattccgc ggatatattc taccgtttca gagcttaaca catctaacga ttcttgatat
     3121 ttttgaataa aatttgtatt ctctgtcatt ttctttaaca agaatacacc aattactaac
     3181 atggcgatta gaaccaatcc aattttaaag ttaatgaaaa agcctaaggc aactgataaa
     3241 acaggtgtta ctatggcttg agcactatct ggaatcaagt gagcaattgc catatgtgtt
     3301 ttagccgcgt tgtcatcaat tgtctttcga atatatcccg aagaatttaa atcaaaaaaa
     3361 cgaaaacttg cttctgttaa accgctaatt cccttttttc tcaaattagt ctctaagcga
     3421 aacccaagga tatgagaaaa taagccagaa aaaatataaa ataaagctcc taaagttaac
     3481 caagcaacag tttgaaccgc ataactctta gctaaatatt catctcctac aataattaga
     3541 gactgaagaa atttgtaaat actgtaatat ccatagactg ttaatacgac agaaaaatca
     3601 gaaaataaag tggctaacca tccgtagtat ttcattttag ggacataact aaataatttt
     3661 ttgtatactt tcattttttc ctcccccgca ttcaatttct tctcttattc gtttacaact
     3721 gttaatgctt tccgtaatag cgtaaattag agatgtacaa acagataaaa ggtaaactac
     3781 gttctatttt ttgtattttt ttaagacttt tcttccaatc aatacaccaa tcatagataa
     3841 acttgctaca actaacactg aaatgattcc ccatatacct accgcatagt ggatcattgc
     3901 atccattgtt gcagcagatt gtcctttatc tatccaatct tgacgaaaac gatctgtaaa
     3961 gaaccatata ggaatccact gtccggcagc actgcctaat tgcattagag cccaagacgc
     4021 aacttgagga attgctcgat catatccaaa tacagataga ataatatcag ctaagatcgc
     4081 catactaatc gaactgataa accaaggtaa gtacgccccg cccattaacg taaaaaataa
     4141 caataaaaca attgtgtaaa tagtaaaaat tccttttcta gggtagttat agctaatata
     4201 aacaaatatg attccagata ataatccaaa aatcccgggg gatatgctgt gaccaaacgc
     4261 cccaccaata ctactaacca ttattgaaaa aaagtaaatt actagggaaa taactgtagc
     4321 taaaccaata aaaatataat cttttgtttt catatatatt ctcctcaatt ctatttcgtt
     4381 atgtcattaa aaatctgttc tatcaaagtt gaatcttcta ctctaagttt tatatctttt
     4441 tttattccac cattttttaa ataaataatt ctattgcatg ttttataaag aaattccgga
     4501 tcatgagtag ctataactac tgcacccttt tcttttaagt ctttaataag gtcagcaatc
     4561 ctttccatat taatataatc caacccactt gtaggttcat caaatagaaa taaattagtt
     4621 tttgacaatg aagctataga aaccaataat ctttgttttt ctccaccgga taaatcaaac
     4681 ggtaattgat ctttcatttt ataaagttcc atttttttta aaattttttc ttgctgatct
     4741 ttactaacta tttcattccc cagggctaac tcgctcctca ctgattctgt gaaaaattgg
     4801 taatccattt cttgcattac taaaaacggc gatgacaaat tttttttatt ttcgattatc
     4861 tttcctttgg tagggcgaat aatcttcatg agtattctta gtaaggttgt tttccccaca
     4921 ccgtttttcc ctaccaaacc aataacatct ccaactttga catcaaaatt tatgtctttt
     4981 agaatatttt cataagtaat ttcttcaact tttaatacat ctgcacttac cttttcgata
     5041 tagggaactt caatcgaact attaaaaatg tcaaatttcc ttaatgcgtt attgttttca
     5101 gtttttaaca actgaatagt tgtagttttt acagttctat ttttaacaaa tagaacttga
     5161 tcaattaatt ctttcaaata gtaaaaacga tgttcagcta taacaatagt aaatccttta
     5221 ttttttaaat cacaaagtaa gtcagcaagt ttcttagtcg ctttgaagtc caaatttgaa
     5281 gacggttcat ccaatataat taatttagag tttagcgata aactacatcc aatagaaatt
     5341 ttctgccgtt caccacttga tagcgaaaaa atgtttttat ctaatatatt tgaaagatct
     5401 aataattcag ctaattcatt gattctttta tccatatgct cttttgtaaa tccaaagttt
     5461 tccatcgaaa atactaactc actcgttgta tttgttgtaa agaattgtcc tctaggatct
     5521 tgacttacgt agccaatatt ttttgatata ctttcttgtg ttaaggattg tagcgagtta
     5581 ttttcaaata aaatttcccc aaccaatttt ccgtcataaa gtcttggaat aagtccattc
     5641 attaacttta atagtgtgga tttcccactc ccagaagaac cagtaatcaa tgtaagactg
     5701 ccttttttta ttgaaaaatt aaggtcaaca agcgcttgtt ccttaacatc cttatatgaa
     5761 aaagaaacat tgttaaattc aaacaattct ttcacctcaa atcaaaatac ttaagaaaaa
     5821 aatagtaacg gaaaacagga tgatatagtc taataagctc atgtttgttt tatagtaaga
     5881 acttcttttc acaggagatt ctatcccctt tgttattgca ttagcggtca tttcttcagc
     5941 aatcatagtt gcacgtataa gaacgggaac taacagaaat tccaaatatt gcagaggctg
     6001 aaaaaaagac aagcgaatgt ttctcatttt taaagattct ttaatcattc ttatttcaga
     6061 acgataagtc ggaaagaatc tgatcactac tgtaatactt aacattattt ttttcggtaa
     6121 atgtattttt tctaaagagg ataataattc attgctagta tagacactaa ccaacaacca
     6181 tgagatcatt acgaccggca acattcgaaa tacaatatat aaggatgtcg caaaaaatat
     6241 agtaaaaaac tctatcttga tagcatcaat aattttcatt actaaaaaca gaactgaata
     6301 aatcaagatg aaataaatac atgtctttat tttacgaaac atcaatacta aaactgtagc
     6361 aataatgaaa caccccactt gaaatatatt tccctttcct aaaaaaataa tgaccggaaa
     6421 aaaaatattt aaagtaatca aggttaacgg attgattttt gacactcttt cacctccaaa
     6481 aaataagatc gcactgttat cttaagctga taaaaatgca ccgttattca gataacagtg
     6541 cgatcttatc aaaaagcata actgagaaca attctcaatg caagcttttt ttaattaact
     6601 cagtaattca atccacccag gaacagaaaa ttgatacaat cttctggtta atagcatagt
     6661 cttttctttt gaatagctat gcttcaatat atcatagaat gtagcgtaga atgagtaaat
     6721 gtagaagtgg atttcttctt cattaaagac taaattttga ttgatcattc cgatagtctt
     6781 taacttatct agacaacaga ctgtcttttt cgtcacaaat gatgtaattt tttcaacaat
     6841 atcttgatat ttggttccag ctgattggaa taaaatcaaa tcgaatatac gtttattctc
     6901 aaaaatataa ttaataaatg gatctaattc attgaagttc tctctccaac ttttctctat
     6961 atctatattt tctaagttta agaggtattt ggtaaactct tcattataca tattaataag
     7021 cgcattaata tgcggctgaa taatttcttc aaataacgct tccttagaag taaaatgttt
     7081 ataaaaagca cccgtagtta cgcctgcggt caggcacagt tgccttatct tagtttgctg
     7141 aaatccttct ttttcaaaat ggcttagtcc actttctatt aacttttggt gagttataat
     7201 ataatctctg gtttctatgg gtacttcctc ctaataagat gaattaactt tattatctta
     7261 cacaggactt gaagtttcaa tcgttaacgt ttattactga actcattcct tagagctaaa
     7321 ctaatgagaa agagcagaat aactgataca aaaagcaatc ctacaagaat taatagactc
     7381 atctaaatca ttcacctttt ctaaaataat ccgagaaaga tttaaaaaaa ttatatccag
     7441 ctattaggat aagtcctatc ataatcactc caatagccat tttcttatcc tccctaaaaa
     7501 taaagtcttt tacttaaaac cttttttgta atcgtcaata aattttttcc ctttatcagt
     7561 taattgaaat cctatatcta aatagtcctc atatctaagt ttagccagca tctcacttac
     7621 agatgctgga ctaacactta gaacttttgc taaatcatta ttttttaatt tcttttttct
     7681 ttttgataat tcctgaattg ctagtaaata aattagtata gtagtcgttt taatttgtac
     7741 catacctacc ctccaaatta tttcgaaagg ccttcagcaa ttttatccag gttccatttc
     7801 atcatcgcat agtaactatc accatcttgt cctttttctg caattgaatc agtaaaaatc
     7861 gttgaataga taggaatatt ggtatctttt gatactgttt tcattggtct atcatccaca
     7921 ctgctttcta cgaataagga gggaactttt gttgtgcgta atttttcaac taagtgtttt
     7981 atttgatctg gtgttccttc ttcttcagtg ttgatttccc aaatgtaagc agaaggcaca
     8041 ttatacgctt tcgagaaata tttaaagcat ccttcacttg taacaatcat ttttttatca
     8101 ttcggaattg aagcaaattt agatttagct tctttgtcta gagaatccaa tttttcaata
     8161 tacttatcta gattttcttt atagaatttt ttattatcag gatctttttc cgctaactgt
     8221 ttttcaatat ttttagcgta aataataccg ttttctaaat ttaaccaagc atgaggatct
     8281 tccttccctt tctcactctg accctctaag taaataacat ctatgccatc acttgctgca
     8341 aaatagtctt tgttttcctc tttgttcgca tttttaacta attttgtaaa ccaagcattt
     8401 cctccagttt ccaagttaac accgttataa aaaatcaaat ctgcttttga agttttttga
     8461 acatcttcag gcaaaggttc atattcgtgg ggatctttcc caataggtac gatactgtgt
     8521 aaatcaattt tatcttttgc tatattttcg gtaatatctg ctaaaatcga attagtaact
     8581 actactttta atttttcgtt cgaagccact ttatctttgt ctttactatt tattgaacat
     8641 gccccgaata taaaaagagc aatcaaaatc cccagacttc ccagtactgc tttccatttt
     8701 ctcatcataa cgatctctcc ttctttctta aaaatttttg ttttggtgag attagaaaac
     8761 taattacaaa taagaatgcc gctgttaaaa caatacttga tcctgctgct atgttaaaac
     8821 tataaccaat aaacaatcca agcaatgatg aacttgctcc gactgttgca gaaataaata
     8881 tcatagtttt caagctattt gcatataaat atgctgtagc tgctggtgta attaacattg
     8941 caacaattaa tatagttccc acactttgca tagctgttac tgctactaaa gttaatagaa
     9001 ccatcaatag ataatgataa aaactgacat tcatccctat tgcttttgcc attaatggat
     9061 caaaagaagt gattaataat tctttaaaaa atagaactac tataaccaac actaaaaccg
     9121 aaacgccaat tgtaatccac ttatcaatat cttgtacagc taaaatattt ccaaataaaa
     9181 tatgaaataa atccgttgag ctatttgcaa ctcctataag gataactcct agtgctaaaa
     9241 aagaactgaa agtaatacca attgctgtat ctccttttat tacactattg ctttttataa
     9301 aagtaataat gatagaagct aatagaccaa atacaatcgc accaataaaa aaattaatac
     9361 ctaaaatata agaaattgct actcctggca aaacagcatg agaaatagca tcacccataa
     9421 gagacattcc tcttaaaata ataaaacaac ctactgttcc agaaacaatc cctataacta
     9481 cagatgtaat tagagcattc tgtaaaaagt gaaagtcata cagaccatta ataaaatttt
     9541 gaagcatatt attccccctc tccaataaat atagtatctc cataggcttt ttttaaattt
     9601 tcttcattaa atacagattc tacacttccg tgagcaatta gatttcgatt caaaagaata
     9661 atattatcaa aatacttctt taccttgctt aaatcatgat gcacaattag cactgttttt
     9721 ccctgatgtt tcaatttttt taacgtatcc ataattattc gttcactaac taagtctatc
     9781 cctacaaatg gttcatctaa aaatatgaaa tccgcatttt gagcaagaca tcttgctaaa
     9841 agtacacgtt gaaattgtcc tccagataat tctcctattt gatgattaga atattttccc
     9901 atatcgacct tttccaaagc ctttgaaact ctttgccatt ctgcattttt aattctttga
     9961 aaaactttca ttccggcata tgttcccata gatacacatt cttttacttt tattggaaaa
    10021 gtaaaatcaa tatcagcttt ttgctctaca tatgcaatct tcaccagttt tttattgata
    10081 tcttcattat taatggagac ctctccctga tgagggataa tatttaacat tgctttaagc
    10141 aatgtggatt ttccagcacc atttggacca ataatacctg ttaaagcacc ttcttgtatt
    10201 tttaaagaaa tctcgttcaa tgctaaaaaa tcgttataac aaacattaag ctttttcact
    10261 tctaacataa ctttctcctt ctaaatgata atgattatca cgatcatatg gtatacaaaa
    10321 attatatttt agtcaaccct aaattttata gtttagattt ttttctgttg ctgaagatta
    10381 gaatgacaaa actatttgaa actacgcgta tagtaagtag ctttcaaaat atataaaaaa
    10441 ggagcgttct caaatgagaa catgctccta aaactacttt attggcgtat ggaaaaatta
    10501 tacttgaagt ggatagcggg ttgaataagt tgataagcat caaaacattt ataaaaatta
    10561 ttcaagacta tgtctgttcc atatacaaat atgaaaatca gtaaactcca tttaactttt
    10621 ttcgtagtgt gtctttttat acatgtttat cgttattaat agtgaatagg aaaaaaacta
    10681 tttttgatca ttgacaatta aatacaattt ttgaataacg atccctgcta ttcgagctag
    10741 aaactcaagt acgccatgac ctgattagcg acaaatactt agagatttct ttttgttctg
    10801 ggaaaacaaa atgccatgac aatagcagca aataatgata cttccgtaag gctcgccgta
    10861 aactggggag aggtgaaatt cctatgtgct tacccaaagc aattcaaaaa tttcataact
    10921 actattttag gagatgattt tatagaatcc agagtctacc tgaataaaaa acaaacctgt
    10981 gcatttctag gaatcgataa taatactcta gataagtgga ttcgtatata taatttccca
    11041 gtaataagag ttggtggtgt agttcgcttt gacgtagaag atattaaaga ctttatgaat
    11101 acatttaaaa taacttgaaa ttttcttctc gccgcatata ctaaaattag tgaaatatac
    11161 tgccttactt tttttaagga gtgaggattt tgcctacaaa gctttcaaat gggaaatata
    11221 aaactaatct gagatatcca aaaaggttta aagaaataac aggaattgca tcagaaaagt
    11281 atcaaaaaac atttccaaat aggcaattgg cgattaaagc cgaaaatgat atgaagaaga
    11341 aaatagaaaa agttttgaga gaggagaatg caaactcatt agaattaaaa ggaaaaataa
    11401 cgtttaaaaa attttacgaa tctaaatggc ttcctagata tgaattaggt caaacaatta
    11461 gatcaaacag gcctcctagt gatataacca ttagtaatac aaaagatata tttcggttgc
    11521 atattttacc tatgtttggt gaatacgcaa tgaattatct aaatatcaat actgagataa
    11581 tatcagatga attaaccaaa aaatcgaaag aatatgcaaa tataaaaatc ataaaaggat
    11641 atgttcgttc catgttcgat attgcagaaa tactaaatta tatcgaattt aatcgaacta
    11701 ctaaaattat tcaaagtatc actgcaccta aaaaaaatgc tttagaagaa aaaagaatac
    11761 aagaaggtaa gcaagcacta tcatcaaaag aacttactaa ttggatagaa gcagtaaatg
    11821 atgattttaa taatcattta cttacttttc atgattacac actttttatg attaccctgt
    11881 atcttggtga tagaaaaagt gagacttacg ctttgcaatg gaaatatatt gattttgaaa
    11941 aacaaacggt ccgtcttaaa catactttag ataaatatca aagaaaaaag tttacaaaag
    12001 gtcgaaaaga taccgtcatt caagtaccag aagttgtaat gacattgcta agtgaatgga
    12061 aatcggtaca agctgatcaa ttgttgaaat taaaaattaa gcaaacgtta gatcagtatc
    12121 tctttactta tactaaacca tctggagaag taaattgtcc tgttcatgcc gattacctaa
    12181 actatcgaat caattcaatt aaaagaaggc atcctgactt agttcattta tctccacaca
    12241 aactccgtca tacttatgca actatagcta gacaaggtgg agcagatatg aatcaaattt
    12301 caaatgcttt aacacactca gatatttcta caacaaagat atatgtgaac acaccagata
    12361 tagttgataa ggctgttttt gaagcttttc agagaggatt gaataagtgt gattgatatg
    12421 tgttcttaaa tgtgttctta aaaacaaaag aagcactttc caaataattg aaaagtgcca
    12481 gaaacgttga tatataaagg atattaacgt tttgagaatt gtgaagcttt acgtgctttc
    12541 ttaagacctg gttttttacg ttcgaccata cgtgcgtcac gagtaagtaa gccagcacgt
    12601 ttaagagcta aacggaagtc aggatcaact tctagcaatg cacgagcgat accatgacgg
    12661 atagctcctg attgtccagc gtatcctccg ccgtttacgt taacaaatac gtcatatgcg
    12721 ccttttgttt cagtaacagc gaaaggttga ttgattactt cacgcaagtc agcatgtgga
    12781 atgtattctt caacatcttt tttatttaca gtaattttgc cagttcctgg tactagacgt
    12841 acgcgagcaa ctgcgttttt acgacggcct gtgccgatat attgaacttg tgccaatgaa
    12901 attccctcct attagattaa gtttgtgatg tctaaaactt ctggttgttg tgctgcatgt
    12961 ggatgttctg ctccaccgta tacgttcaat tttgtaaatt gtttgcggcc taaagtgttt
    13021 ttaggaagca taccttttac agaagtttcg atcaaacgac gagaattttt agcacgcaat
    13081 tcgccagcag tgattgattt caatcctcct gggtagttgc tgtgacggta ataaattttg
    13141 tcagtcgctt ttttacctgt taatttcact ttgtctgcat tgatgacgat gacgaaatcg
    13201 ccagtatcca aatgaggtgt gaaagttggt ttatttttac cacgtagtac agatgcaaca
    13261 actgttgaaa gacgtcccaa tggaacatca gttgcgtcta ctacatacca tttacgttct
    13321 acttcgcctg gtttggccat atatgttgta cgcacgttat tttcctccaa tttaagttct
    13381 gatgtttgac atacacttcg agtttccggg gctctttgtg gggcaaacaa taccatttaa
    13441 catattaccc gctaatctag tcaatgtcaa cgtttttctg cttgattgaa aaatttatat
    13501 gaaactttac ttcttttctt tcttttatat aaaagggtcg tttcctgtca aaatgaaaaa
    13561 caaaaagagg ctgtgacaaa agtcttgtca caacccctta ttttgaataa acggtgttct
    13621 cctgccaact cctcggtcac aagtcacaat atccataaaa cataagaagc tgttttttaa
    13681 aattgtttct tgtgactcgg agctgaacgg caggcttaca acctcttggt aaacggtgtt
    13741 caatcagctg accctcggta ttagctcaaa aaacggcaaa atatgaggaa ctattttgac
    13801 gtttgtttct ctaatactca ggtcaaaacg ctgatttcac aacctctttt tcggtcaagt
    13861 tcaacaacag tttttacgcg tcgatctctg gcgctttgcc caaatgagct tggatcatcc
    13921 agatacgttt ttctgtggct gttttgaatt cgataaagat atcttgtgtt ggatcgtcgc
    13981 cttcttctcc agaaacttcg attccttttt ggtaaagatc tgctagataa cgatagcctg
    14041 caacaagtgt ttccaaacgt tctggaatag tacgatccca gcggccaatt tcatcaggga
    14101 tttttgtgtt atctgcaaat tcacgcaatg tagaataagg tgaaccgtct aatgtgatca
    14161 agcgttcgga tacttcatct aactgagcgt tcaaatcatc catgaactca tccatcattg
    14221 ggtgcaatgt aaggaaacct ggtcctctca tgtaccaatg tgtttggtga acaacaacag
    14281 acatttggct tagatctgca actaattgat ttaatacttc tttggtttgt tggaatttca
    14341 tctttcttgc ctcctaacat tttcttacac ttaaaataat agtagacttt caatgaaaag
    14401 tacaacaaaa tcatttacct gagaacgatt ttcagttcgc ttttttatcc gtaaagccaa
    14461 tcatttttca gaatcagcta attcttctcg cagttgagca aattgctgat aagtctgtgc
    14521 taaatgcttt tgtgtcgaaa caaataatgg atagattttc tgatattttt tatgatcagt
    14581 ttcttgtact ccaacttgct ctttcaattc tatcaattcc tgtgcttcac tgatactttg
    14641 gatctttccg atgcttctcc agcctaaaat cacagctcct aatgcggatg cttctgtgac
    14701 agtagggaaa acaagcgggt aacccagtac atcagccatc atctgacgaa aaatcggaga
    14761 ttgagcaaac ccgcctgtag cacggatctc tgtgagctga ttttcctggc aaactccttt
    14821 taaaatccga taaaggttga gacagatccc ttctagaatc gccttcatca tttctttttc
    14881 tgtatgttgc cgctgtaaac cgataaaact gccaaacgca tctgcgtccc aaagcggtgc
    14941 tcgctcacca agtaaatagg gatgaaaaat cagaccattg gcacccgctg gcacaaaagc
    15001 gatagccgaa agcagtgcat catatggatt ttctcctttt tctttagcag attgaacgac
    15061 ttcctgtaaa aatgtatgcc ctgcccaatc aaagatgcca gcaccattgc tagtcgctcc
    15121 tcccactacc caatgtttct gatccagaac ataacaaaac gtctccattt ctggatggaa
    15181 ctgaggctga gtagtaataa aacgaagtgc tccgctcgtt cctacggtca aagcggcgat
    15241 cgtatcacca aaagcaccta accctagatt agaaagcggc ccgtctgccc ctcccaaaac
    15301 aaatgatata tcatttggca aacctagttc tgctgctgtt tttgatggta gacctgttac
    15361 ttgataggtg gaaggaaaga cttgcggcaa ttgctctatt cgaatgttca agtagtcaag
    15421 cacttcttct gcccattgga attcatggat atcaaaatat ccagttcctg aagctgttcc
    15481 gtagtcgctg atcagttcgc caaacaatct ataaaagata tagtctttga taccgacaaa
    15541 ataagcagtt tttttgaata attcaggttg gctgttttgc agccacttga ttttgttcaa
    15601 tggtgtcatt ggatgaatcg gcgtcccagt ctgccgataa aattctttgc caagcacggt
    15661 atatttcagt ttttctgctt cgtccgctgc tcggttatct gcccaagtga tcattcgaga
    15721 taacggtgtt ttctcttcat ccaacaatat caggccatgc atcgcagagg agaaggagac
    15781 aaattgaatg attgtgtctg aaggcaactg tttaattact tcttttataa cggtgcagac
    15841 accttgaaag atcaattcct gatcttgttc tgccatccct tgtgtttctt ggatcaaagg
    15901 gtagcgaaaa taagcggatg ctgcttcttg ggcttcatct gtgaaagcaa ctgctttggt
    15961 ttgtgtggtt ccgatatcta atccgattga aacgttcatt ttttcacctc aaattttatg
    16021 tatgtacaaa aatttagttt aagcttgcgt atcttggttt tgaaaagggg gagatggcgt
    16081 gttactttat tttatcattg gttgctgtat cggatcattt ttatgtttaa ccgcccagcg
    16141 gattccgtta ggtcactcta ttatttatcc tagatctcat tgtgtcaaat gctgtcgttc
    16201 gctttcttgg tatgaattga ttccgattct ctcgatcatc gtccaacgat ttcgctgccg
    16261 ctattgcagg tgtcggttgc ccttctacta tttgttggca gaagccttat gtggtggtct
    16321 ttttgcctgg ttctttactt tttcttccgc aagaaatttt tcgacgttca tatggatgct
    16381 ttcggctttg ctattttcgt tgatggattt attttacttc atggtccatt ctcacacgct
    16441 attctgttct tgggcaattt tatggatttt ttggttgcag gccggcgtct tccaatggca
    16501 aagtgtgctg ttaacgttta tcgtaggcgg tgtgtgcctt cgctatggtc aatcctatct
    16561 cggagctggc gatactctgt tgatgctttc ttggagtggt ggcttgtctc tagaagagtt
    16621 gcttcagatc ttgtttcttg ctagtctgtt tggaatatcc gttttttctg tttactttat
    16681 ctgtcataaa aaaaagttgg aaaagctgcc tttcattcct tttttgagca gtgcgttgct
    16741 gattgtcctt catcttaggt attgaaaaaa ggttgtgaca aacgacttgt cacaacctct
    16801 gatatatagt gttctcttac caaatccttg tgacttggag ctaggcgaca ggttcacaac
    16861 ctctccacta aatggtgttc aatcagctga ccctcagtat tagctcaaaa tgctggtttt
    16921 acaacctctg atatacggtg ttccataagt agctttttcg gcttcttatt tcttgacgcc
    16981 ccaatgtcga cgaaggaaac ctgcacgtcc agaaccttct cgataaagat tgtaatggag
    17041 cttgtttttc ttgtaataat cctgatggta atcttctgca ggataaaatt cgcttcttgg
    17101 ttcgattttt gtcacgattg gcttatcgaa tcgcccgctg tcttgcaatg cttgttttga
    17161 acgctctgca atatttcgtt cttcttcatc attatagtag atcactggac gataagaatc
    17221 accgcgatca gcgaactgtc ctaatgcgtc agtcggatcg gtttgctgcc aatagatttc
    17281 caccaactga tcataagaaa taacttctgg atcatattcg atctctactg cttctgtatg
    17341 ccctgttgtg cctgttgtta cttgttcata agttggattt ggtacatgcc cgccagtata
    17401 accggatgtc acagaaatga tccctggctg ctcttcaaat ggtttgacca tacaccaaaa
    17461 gcatccgcct gcgaaaactg ctttttttgt catataaaat cccccttttt tctcatcata
    17521 ccttgatttc caaaaggagg ataacgtttt gcttgtgaca gaaaaaacgg aaactattat
    17581 atacgtgata cggcttctga taaatgccaa aacgaacgta cttgatcaaa aacggttaaa
    17641 tcaggaatca catattcagc agaaggaaga tttatgtttt cacctattcc caatacatgc
    17701 atgttaccag ccaaagctgc ttgacaacca gcttctgaat cttcaatgac caagcatgca
    17761 tttggcggta cattcaattg ctgtgctcct ttgagaaaaa cttctggatc aggttttgct
    17821 tttgatactt gcgtgccgtc aataaggaca tcaaaataac ttgtcaaatt cgttttttct
    17881 aaaatgaggc gtgcattttt gcttgctgat cccaatccga tcttgatgtt tgtttttttc
    17941 aagtactcaa gaacagctat tgcaccaggc aaaacggatg tttcatccat tttttgaatc
    18001 gcttgaagat atagattgtt tttttgctca gcaaaggctg atttctcttt ttctgagaat
    18061 ttctgttcca tttttccaaa tgacagcaat cgctctagtg actcggttct ggatattcct
    18121 ttcagttgct cgttttcttt ctcagtgaat ggaatggaca gctggttagc taaaaccagc
    18181 cacgcttcgt aatgataacg agctgtgtct acaagaacgc catctaaatc gaatagtgct
    18241 gctttgattt tcatcgatta cctccttgaa aatgaacgac ttgaagtaaa gatgcgatgt
    18301 ttttagctgt gcgatgaaga ttatcagtgg cttgctgtaa agcttcttgt aaaggcacag
    18361 ctgcaggaag gatcggaaaa acggccgaaa tgccaacatc tgctgctttt tcgcttcctt
    18421 taccaacact tccgcaaata gctatcacag gaacgccctc tggtactctt ctagcaatcc
    18481 caactggcgc ttttcctttt agactttgtc catctagacg tccttcgcct acgattacga
    18541 ggtctgcttg ctgtacccgt ttatcgaact ccagtaaatc caatacgtag tcgatgccag
    18601 agactatttt ccctgaagca aatgctaaca gtcctgcacc cattccacca ccagcactac
    18661 tgcccgatgc ctcgattact tgaggggcaa atctcatata aaaatcatgc atggcttggt
    18721 cagccactgc taattcattt tctttcaagc ctttttgtgg tccgaaaaca aaagaagcgc
    18781 cttcttttcc gcataaaaga ttagtaacat cagtaatgac agtgattttg gctttaatca
    18841 gctcaatagg cacgttcgtt ttatcaactg tagcaatttt acctagattt tctcccagtg
    18901 cttctacttt ttgattatct tgatcgtaaa aagcataacc tagaccttct gccatgccga
    18961 tgccgccatc gttggtacta cttccgccaa cgccgatgat gatgtgtcga acaccttgtt
    19021 gcaacaattg tcgtatcagt tctcctactc ctcgcgtgga taacctaagt ggctgttgtt
    19081 ggtttagtgg gacatctttc aatccgacga tttctgccat ttcaaaaatc gcagtttgac
    19141 cgtctgaagc gtactggatt tctttgtttt tgccaaatgc attagtaacg actgctgttt
    19201 gccgcttcaa ccttaaactg tctgctaatg atgaaaccgt tccttctcct ccatcgccta
    19261 taggtagcag atcatattca gcctgaacaa aaacttcttg aaaacctgat tgaatggctt
    19321 gtgctgcctc taaggctgtc agactttctt tgaaagaatc tggcgcaatc agaatacgca
    19381 ttattttccc ctccgttcca agatcagttc tttttccagc tggtattggt ctccattgac
    19441 aaaaataggt aatgtttctt tcgtctcttt ttttattact atttgttttt ttgtcaaaac
    19501 aaatagcaac ctgtgatttt tccaaataaa aggaaaagaa attttttccc atgctgaagg
    19561 aagtcttgga gaaatagaaa gagttccatt cgttactgta atacctgaaa aaccaaatat
    19621 aactgctagc catagtgctc ctaatgaagc agcatgaatt ccgtcatctg atgaatgcgg
    19681 atctggtcct aaatcaatca ggcacgcttc ttggaataaa tgatacgccc attccatttc
    19741 ttggatacgg caagcctcta tggcgtgaat cgctttactc aacgatgagt cgtggattgt
    19801 tttgttttca tagtaatgca aattcttaga aacgattggt tgagaaaaat actcaggtaa
    19861 taagtataac agcatgacaa gatctgcttg cttgctgact tggagttcat ttacttcgct
    19921 acgggcataa tcaagtaaaa tagactggct gccaggacgt tctttgtaag tggtcaaatc
    19981 aataactggc ttactgagat acgtgtcatc ttgcggaatc agctgttctt tgttaggcac
    20041 tggcaaatat aacttatcta aaaaattttg acattctttc aaaaaaagcg gatcgccagc
    20101 ttgatattta acagctaagg ccacattata atgcgccaga tagttcgtat atgcattatt
    20161 gtcgatatat tctgtgtatt catctggacc aatgactctg tgtatctcta aatgatcatc
    20221 gacttttaca gctctgctca gccaaaaagc tgctgtttca tgaatgagcg tttttccctt
    20281 tgtttctaaa aacgattgat cctctgttgc ttggacatac tcttgaacgg cataagcaag
    20341 gtctgcactg atatgatgtt ctgcggcagc tgaagcaact tcttgtcttg ttccggtttt
    20401 gatattcaat gcagcaaatt tgggtgtctc ttcctgacca gtgtgtgcgc tttcccatgg
    20461 gaaaagtgca ccttgatagt gataatctct tgctttttgc cgtgcgcctt ctaatcgatc
    20521 ataacggtac agcagcaaat tctttgctac ttcaggaaac tgatataaaa agaaaggcag
    20581 gataaaaatt tccgtatccc agaaaacatg ccctttataa ccttcgccag ttagtccctt
    20641 cgcccctaca ctaacacttg gatcattgcg gggagtcata atctgtaaat ggtaagcagc
    20701 gaagtccatc gctaactgat cgaaagaaga atcgccttgg atcgttatcc gatgctgatg
    20761 ccaaaaatct gtccaataag acatcgactg ctctaaaatt tctgtataac taacgctttt
    20821 ctgggcttgg gcaatacttt tcttaaacgg gtctgtttgc tgaagagagg tagagagcac
    20881 gatgatcttc tccagcgtaa aggacgtatt ttttctgatc ttttctgaaa aggaagcggt
    20941 gatccggcga ttttttgctt ggaatacacc tgctttattg aatttagctg caatcccaat
    21001 atcaatttta ctttctgttg tctggtagga tgcactgatc aaatcgtcac caaatactcg
    21061 cagttctttt tcaaccaact gctgtgtacc aaaattggtt tgctgtccat cgatacccgt
    21121 tttcacatct acttggcagt cttcggtcaa cggcttgatg accactttca gggctgcctg
    21181 atggagatta ttttgatcag caacacgaca agtaatgact tccacttttt ttccttgagc
    21241 agtttcaatg agcgcatagc gtttaagctc tcccatttct aaatctagtg cccgctcgta
    21301 tgcaagaact ttcatcgtct caagagaaaa atgttcaccg tcgattttta tttcccatct
    21361 gatgacatct gggagattca ctaactcggc tgattctgct cctaaaggtc ggttatatac
    21421 accagctgca aacattcccc ttacttgatt ccgatagtct tcctcctggc ttcctctgat
    21481 accaaaaaat ccatttccaa tggtcatctg actcgcatac ttattgatat actctaaatc
    21541 aaactgtttt tcttctactt tcataaggta ataacttctc ctgtttctgc cgaacgatat
    21601 aaattttcca ccagattctg gatgacatat ccttgccagc catctgcaat catctgagtc
    21661 tggtcaccga gcgcccgatt cacaaaatga gccatactta gttcatgtgg gtcattaact
    21721 ggcgtggtct tttgtaagga aaccagcttt ccatctacat cttcgaatag ttcgcctgga
    21781 tacagattag cacctgcctg atcgccacaa aaagccacat tcagtttgtc ttctctgata
    21841 ttcaaagcaa acgaagtatt cagttggatg atcttgccgt tttctaattc cagtgtgcca
    21901 aaaattgcat cttctactgt gaatttgctt ggatcccatt tcccgaattt ccctgaattt
    21961 tttttcgtac caattttttg gaagctgtac gcccgaacgc tttttacttt aggaaatcca
    22021 gtaatataca tcgctgtatc aagcatgtga atgccataat cgatcaaagg cccgcctcct
    22081 tgaagttctt tgtcgataaa cgttccccag cctggaacac cagaccgacg aagcgcattc
    22141 gcttctacgt aataaatatc cccgagaaca tcaagatgat tcttgatcat tgttgcttcg
    22201 tcagaaaaac gatgctggaa tccataagcc aaaatctgcc cgtttttatc ggctgtctct
    22261 gccatctttc ttgcttgatc agctgtcata gctggtggtt tctcgcacat cactgaagca
    22321 ttagcttcga gggcagccaa tacatgttcg aagtgaaagc gattgggtgt acagacactc
    22381 acgatatcgg gagaaatctc attcagcatt tgtgtaactg atgtatacgc atgagaaaat
    22441 cctgtacgtc ggcaaaagtc ttctgctcgc tggctgactg gatcaaccac agcggccaat
    22501 tctaagacat ctgatctttt ttgataataa gcagcatgta ctttttctgc tacttgtcca
    22561 gcgcctatga ttgctacttt cttcatttat ttctccctcc tttgcttatt tacttattgt
    22621 tattctttct ctattctttc tctaaatcat ttaaatgacg cgctgtttcc agacagtttt
    22681 tcaagtattg caatgattcc tgataaattt tcacttcatt ttctcctcgc aaacgacatt
    22741 catataccat ataaccttca tactcatttt gaagtagcgt ttgaaaatgg cgagtaaaat
    22801 caattgcgcc agatcctggt tggtagcgat gattatctgc taaatggata tgacccacat
    22861 actctttttg cgtagtcaat gcttttgaaa tatcatcttc ttcaatattc atatgataaa
    22921 aatcagcgat gatctgcaca tgtttcaatt gattagagtc aatgtaatga cgcgcatccg
    22981 ctaacaaatt gatcatatgg tcttggtatc ggttcaaagg ctccaaataa ataaccgtac
    23041 ctgtttcttc ggctacttta tctaaataac gaagtgattc gctgactgct ttatagtctc
    23101 cttctttgct tcggggtgaa accatcggcg gtaatcgata agtgaacatt ccccaagctg
    23161 ctgggataac gattcctttg cctccaacct cgcttagtgc acgcaaaatc tcagcaattt
    23221 gacgtacacc attaagacgc cgttcttcga taaagtcacc gatccaccca tcgtagccgt
    23281 tacaagctgt cactactggg atccctgttt ctttgattgc ctgtttgact gtgtcgatat
    23341 tttctattaa caacttaccg tcgatttcgt attcttcaaa tccacagtct ttgataaatc
    23401 taaatttttc taaaatgtct cttgggaaaa atgcttgatc ttgcgtaccg aatttcattg
    23461 ttctcctcct cggacttgaa cgcccatttt gatactttct tctgggtgct ggtctacata
    23521 acgcatgtaa gcttctgcgc tttctgcaaa tggcacaact ggatcaatga tttcttcaca
    23581 atccaagtaa ccgttcatca ataattccca acatgtgtcc tcgattcgtt tacggttcca
    23641 tcttgggtaa tctgggtttg gttcactaga tgctcgagca aagacgattt ttgcattatt
    23701 gaaatgtgct tcgcgaccaa gattaaaccc agctggaaaa ggtttagcaa aggcaacata
    23761 agaaatgatc ccgccatagc cgatccctct taaagaagct tgtaatgcgg cagcactgcc
    23821 actcgtttca ataactgaat ctactccacg tttatctgtc aattccttta gacgaaaacc
    23881 gacatctgct ttcataggat ctagcgtgta atcggcaccg tatttttctg ctaattctct
    23941 acgctgcgct atgggatcga tcccaacgat ggtagtagca cctgcttttt tagctaattg
    24001 aatcgcaatt tgcccgatag cgcctaaacc gaagattgct acagcatcac ctgctcgaac
    24061 atgtgcttcc cggataccac ctaaagcgaa ttgagcagga tcgtaacata ctgcattttt
    24121 ccattgatcc aaccgatcaa gtttacgtag tttataatta tcaacaccgt ttgtaatcag
    24181 cgtttcacga atgggtccat aagtgcagac taactctcct ttttgatact ctgtcaccaa
    24241 actgcccgtt tcttcgatcg taccgacaat catatttcct aattgaaatt cgccaaatac
    24301 cacaccgcgt tggcttcctt cttctcttgg caagaatagg cgccattctt ctgaaaattc
    24361 ttcatcgata aatggactta atcctctaaa gtcgacaatt tctgttcctg gttttggagt
    24421 agcgaaaatt actcggatac gtacttcgtt cttttcgatc tcacggtctt cgtaatcttt
    24481 gaatccggct attcttggtt ctaatgcaac taattttttc atcttttctt tcctctttct
    24541 tttcttttta tccttttacg ccgccttcga ctaaatcacc tttaataaat cgttcagata
    24601 acgcatacat aatgatgact ggtaaagcgg tcaccaatga agcggccatc attcgtcccc
    24661 aaatgtagtc tggtgtactg aataatgtgt tcaaaccgat tggtaaagta aatttttcgg
    24721 aggtcgacag gaaaatcgaa gcaaataaat aatcattcca cgaaaccata aagcaataaa
    24781 cgaagacaga aatgattcca gaaatagcca atggtataat gatccgaata atgatctgga
    24841 tccggctaag accatcgatc attgctgctt cttcaatatc agtcggaatc gtgtcaaaat
    24901 aactttttag catgaatatc gcagtaggaa gtgtttgaac gaccatcgtg ataatcagtg
    24961 ctgtattggt atcatacaaa cctagaccgg aaatgatttt aaataatgga acaacgatca
    25021 aaataccgga aaacatataa acggtgtaaa agctagcatt gattttcgag cgagctttga
    25081 agcgtagttt tgacaaagcg tatgccccgc tgatcccaat cactactgca gttacggagg
    25141 ctatcaagga gatcactaaa ctatttttga aatacatgat gaatggaaaa atctcagcat
    25201 tgaaaatatc ctgataatgt tcaaaagtcc attctttcgg aaaaatagtt tgtgtcgttg
    25261 caattgcttc ttgtgaatcc ttgaatgatg tcatcagcat gatcaaaaat ggaaacaaat
    25321 tgacagctaa tagcaacaaa atcgtcgcgt aaaataagat tttcctgctc tttttatttt
    25381 tcatttcaag tccaccttct ttcgtgagag caagacgacc gctgcaataa tgatgaacaa
    25441 tagaatcgat atagctgctg cttttccaag atcattgaac gcaaaggctg tctgatacaa
    25501 ataaacgcct aagatattga ctttattcgt cagtagatag acatctgtaa acatatagaa
    25561 catccagata gatcgtaaaa caatcacagt tgctagtact ggtttgattg ccggcagtgt
    25621 cacgatcttg aacttctgcc aagcatttgc accgtccatt tcagctgctt cataaagcgt
    25681 tttgtcgact gtttgcaaaa tagccaaaaa tgaaataaac gcatacggat agtatctcca
    25741 gatagcaaac aaaacgacca taaagaagct agcaactggc tggtcgaacc acagcggtgc
    25801 ttcgttgaaa agctgcaacc tatctactaa cagatcattg acgattccat aactattatt
    25861 gaacatatat ttccaagcaa aaatcaaaga aatagatggc gtaacataac ttaataaaat
    25921 cagtgaacgc accgttttcc taaatctgaa cttgcggtta agcaaaatag ctacggctaa
    25981 tcctaaagct gtactgcctg ctacaactaa gaatgtgtaa gcaactgtta aacctaaaga
    26041 acgataaaat tcactatcac ttaaaatttc aagataattg cttaatccga taaaaacggc
    26101 gtctgagtta ggattcagcg gcatatcgaa aaaactgatc tgaatattgg aaagcattgg
    26161 aatcagcact aaaacagcta ataaaagcat gcttggtgct aaaagtccca tggctaattt
    26221 taagtcagaa tgtttccttg gacgattcat tgaaacctcc ccctgataat ctgccaatag
    26281 ctatctactt ttttcgaaca ccattggcag aacttcttgt tatttcgtat ttttttgtgc
    26341 gtcagctaaa gcttgatcga catcttcgtt tccgacagtt acttggttca ctgcttttcc
    26401 gatcacacct gagcttgtga tatctcccat tttcgtgaaa ttcttttccc cgactagtcc
    26461 aaagacttgt acttcatcaa aagcactagc aatctcagat ggcagctcgc caaaagactt
    26521 gatgacttca ttttctttgt aaccatccag ttcgactact tgtttattca ctggttgagc
    26581 accaccagga gacatcaaga cccacttttc catgtttttc ggttgagata agaaagctgt
    26641 gaatgcttca gcagcctctt tttgtttctc gtctagacca gaagaaatcg tcaatccaga
    26701 tactgtgcca tagacagctt gatctttttg tgttgggata gcaaaaccga tattttctga
    26761 gttccccgct tcataaacag aaggcaaaat gtaagttgaa tagattgcca ttggtgctgt
    26821 tccattcatg aaagcatctt tgatttctgt cacatcatta gagcctggca tcgtgtattg
    26881 ggataagttt tgataaaacg acaatgcttc tctcatcttt tccgtatcga tcgttacctc
    26941 gccttcatca tccaaaacat ttgctccatt tgacaaagca aattgagaga acgcttgctc
    27001 actcattgtt ccttcagcag taggcatagc aattccatat tgtttattct ctttatctgt
    27061 gaaatgttga gcaattttta aaatatcatc ccaattttct ggttctgaaa aaccagcttc
    27121 tgataatttt tctttgtcat accaaatacc ttgaacccag ccgctaattg gtgcagcaat
    27181 atagcttttt ccatcttctg aacgaacaag gtttttggct ccgtcataat aattatcttc
    27241 tccagcttcc tcgataacgt tttggactgc cgtctgatca atcagttcat ctttatccat
    27301 cactttagca aaatcctgac tgacttccat tacttctggt aatttaccgg aacgagctaa
    27361 cgtaacgatt tttgtattga acgctgattc ttctaccggc acttgcttga ttttgatgtc
    27421 tggattttct ttttcaaaat cagcaaccag ttcattgatc acacttaatc gttcctgttc
    27481 gacagaagag tgcatgaatt gaatcgttgt ctgttgtgat tcgttagagc tttgactttc
    27541 accacaagca gtaagaagaa atccagtagc tagcagacag gtagtaacaa taattttttt
    27601 catttttttc cacctcttat tttattattt tcagccacat atattcatac ggctggaata
    27661 tcaactttcg ttcttgactg caatggataa tttgtccaga aaacaggtct aggtaatctc
    27721 cttttggtaa aagataacct gctgattgat tggaaaaatt atggataact aagactttct
    27781 cttccttcgc ttcgcgaata aaactgataa aagaatctgc tgagtccagt aagtaaatca
    27841 aactattcgg atggaaggct ttttcttttt ttcgaataga tagcatctgg gtcaattcac
    27901 catatactgc tgttcttaat ggatctgtct gcaactcttt ctcaatatct gctaacaaaa
    27961 atttttgccg attgattttt cgattctctc ctgttttttc tacgcctttg taatcatttc
    28021 gacttcccag cacactttga atataaattg ccggcacgcc tggtaacgtt accaaaaatg
    28081 aatgagcagc taaaaagcgt ttgcttctcc attccgtcgt atctgttgga gaacttaacg
    28141 catctagata cgtagcgttt atctcatatg gactggtcgt tccatctggg tttttcttgt
    28201 agtttactcg ggcaccctct ttcttcagct gttccactaa ttcaaggatc gttttttccg
    28261 gcagaatacc tcgtaccgga ttcaatccga taccgtcatg ggaggctaaa aaattaaaaa
    28321 aagttgtttt tttcgatgcg aaatcgatgg tttttaacca ttctttcaac ttacttgtat
    28381 tacccgaacg aatcgcttga agggtcaaag gtggtagcga aaactgatag accatctgtg
    28441 cttcattctc gccattccca aaataactga tattatcttt gtgtggcaca tttgtctcag
    28501 tgatcaatac cgttcctgga gctgcttcat cagcgattgc acggaataat tggatgattt
    28561 tatgcgtttc tggcaggtgt atacaagaag ttcctagttc tttccataaa aatcccacag
    28621 cgtccaagcg aataaatgaa gcgccttttt ctaagtaaaa caaaagaata tcgatcattt
    28681 tcaataaaac ttctggattt ttaaaattca agtctacttg atcttcgcta aaagtcgtcc
    28741 atacgtgttt ttctttcccg tttttgtcaa tgaacgttgt aagtaacggg ctggtccttg
    28801 gtctagtcac gctgcttgta tctgtatcca gtggcaaatc aataaaaaaa tctttgtacg
    28861 tatcattcaa gtttaaatat tcctgaaacc aagtactttc ggcagacata tgattgcaga
    28921 caaagtcgaa cattaaccgt gtttcgcttc tcatttcttc tatatcttcc catgtgccca
    28981 aacggtcgtt gattgcctga taatttacaa cggagaaacc atcatcagat gtatatggga
    29041 aaaatggcaa gaaatgtaca actctaaatg tatctgccaa atacttttga taaaatttct
    29101 taaatgtacg caaagttggt tgctgctctt caaaaaattg atccccataa gtaatcaaga
    29161 aaatatcttc ttcgtcccaa tgcagtttgc ctgcctggcc tttcttctgc tgataaacac
    29221 tgactttttg ttcaatcata gaggcggttt tctcagacaa atctgagtcg cgataaatcg
    29281 cttgtaaaag ttttgtatac atcggattcc cctttctttt ttgggagcgt tcccaacgat
    29341 ttgatcatat tatggaaaac gcttactgtc aatagcattt ttttaaataa ttttgatata
    29401 cacaaaaata caaataagaa agcttatgta tcaataatta atagcttctt aaaaaaatat
    29461 ggtctctcat gatcatgcaa gctattccta aattttcaaa atcatagaca aaaaagaatg
    29521 tctattgtac tatggaatta ttctaaaacg ctcccaaaaa ggaggtgcat ataaaattga
    29581 aacctactat ttatgacatt gcgaaaaaag ctaatgtctc aaaatcaact gtctccagag
    29641 tattaaataa ccagccaaat atctctgata aagcaaaagc aagtgtactt caagcaatca
    29701 aagaattgaa ttatgaaccg agcaaaattg caagaggact gtcaggaaat ggttttgacg
    29761 cgattctagc attatctcat cgtacttctc atacgaccta tggcaatcct tttttttcag
    29821 atgttattca atccatttct gccgtttctg aacaaaataa cttcgacttg attctacaaa
    29881 cggcaaaaac accagaagaa gagctagaaa aatgcgcgaa aaagatccaa gaaaaaatga
    29941 tccgaggaat tattatttta agctctacga tggatgaaac acttcttttt gaattggatc
    30001 aatacaatat tcctattgtg gtaatcggaa aagtcggaaa cgactacacg aacatctact
    30061 cagtggacac ggataatttc caggatagtt acgatttggt ggaaacactg atcaagaatg
    30121 gacaccgtca tatcggctgt ctccatgctc cgacagaagt ttatgtatcc aatgatcgtc
    30181 tcaatggtta ccgtcaatgc ttatttgatc accacctaga tatccgtaat gaatggatta
    30241 tagacggcgg ctttcgctta gaagatagca tggcagcaat gaatgattta atgaagctac
    30301 gagaaagacc aactgcgatt ttttctaccg atgcattaaa atcattgagt atttacaaag
    30361 tggcagaaca atctggaaag cagatacctg atgatctttc gatcgcagga ttcagtgatc
    30421 ccttattgtc ctccctattt tcgccacagt tgactcggat agacattccg acaaatcagt
    30481 taggaatcaa agccacagaa cttttattcc aactaatcca ccaccaatac cctgaagaaa
    30541 aaagaatcat tctttcaacc gagattactc tcacagattc gatccaacaa caagaaatgt
    30601 agaacagaat tataaaagaa cagaaacact gaggataagt cagttactct gttctttttt
    30661 caatcactca tccctcgtta ccccctcccg gtattttagc gaatttttct tattttccat
    30721 ctgttttaga tagctgtgga taactataat gtttgtgtta aactattttt tccacaggga
    30781 agaaaaaact atttttaacc acgttgtgta taagttttta tagttcgttt ttttaaacac
    30841 atttaccctt atttcaatcc atgttatttg gggatactta tctctaaaac ataaatagaa
    30901 taacagacat gtctactggt tttgttttat tcgaggaatt attcaataat agctagcaaa
    30961 atattaaaaa taaccgctct atcttcccat caatttattt tgcattcaaa aaatgatcta
    31021 tctttacttt tgttgattga cttctcttgg aagatgatcg ttccgatacc ttaattggat
    31081 acgaaccgat gttgttaaga tcctttccca cttttcttct agttagacaa attattttag
    31141 acaaaccaaa aaagaggttg tgacaataat ttgtcacaac ctctttttta aaatatctat
    31201 tttgtttgat tgttcattgc gtcttctgca gccatttgcg cttcaatatc tgaaatactg
    31261 tagacatttt cgcttgctac accgacttct tttggttcca tgtcacgata acgttgcata
    31321 ccagtaccag ctgggatgat cttacctata ataacatttt ctttcaaacc aagaagagga
    31381 tctttcttac cgcggatcgc agcatccgtc aagacacgag ttgtttcttg gaatgatgca
    31441 gcagataaga aactatttgt ttctaatgat gcttttgtga tacctagtaa gacaggtcga
    31501 gaagttgcag gaacgcctcc tgagactagc gtattgtagt tttgatcttt aaagtctgcg
    31561 atgtctaata atgtacctgg taagatatct gtatcacctg gatccattac acgaacttta
    31621 cgcagcattt gacgaaccat tacttcgata tgcttgtcgc cgatttctac cccttgcata
    31681 cggtaaacac gttgtacttc acgaagcaag tagttttcaa cagataatac atcgcgaact
    31741 tgcaacaatt gttttggatc gattgatcct tctgtcaatg gtgcaccacg gtggatcata
    31801 tcgccttctg ctactttcat acgtgctgta taaggaacag tatacgtacg tgtatctgtt
    31861 tttcctttga ttgttacttc tttggtacgt gttgcaggat cttctgagat atcgatgaca
    31921 tcccctgtta cttctgtgat cactgcttgc cctttcggat tacgtgcttc aaagatttct
    31981 tggacacgag gaagaccttg agtgatatcg tctccggcaa ccccacctgt atggaatgta
    32041 cgcatcgtca actgagtacc aggttcaccg attgattgag cggcgatcgt accaactgct
    32101 tctccgactt caacgtcaga gccagttgca aggttgcgtc cgtaacaatg tttgcagaca
    32161 ccatgttttg tattacatgt gaagacagaa cggattgtca ctgtttcgac acctgcatca
    32221 acgatttgtt ttgcgatgtc ttcggaaatc aattggtttc ctgcaatgat caattcattt
    32281 gtttcaggat gacgtacttc tttacgggtg taacgtccca ctaaacgatc ttctaatgat
    32341 tcgatgattt catttccctc gcggatggct tggatttcaa gacctcggtc agtaccgcaa
    32401 tcttcttcac ggatgattac atcttgggcc acgtcaacca gacgacgagt caagtaacct
    32461 gaatcggcag tcttcaaggc tgtatcggtc atccctttac gagccccgtg agttgagata
    32521 aacatttcca agacggatag tccttcacgg aagttcgaaa tgattggcaa ttccatgatt
    32581 cgaccattag gagcggccat caaaccacgc attccggcaa gctgtgtaaa gttggaaatg
    32641 ttaccacgag ctccagaatc agacatcatg aagattggat ttttcgcttc caaaccttcc
    32701 atcagttttt gttggatgct gtcttttgca gcattccaaa cagcaatgac acgttcataa
    32761 cgttcgtcat ctgtgatcag accacgacgg aattgttttg taatagtttc aacttgttta
    32821 tgggcatttt caatgatttc ttgtttttca ttcagtacac tgatgtcagc gatccctact
    32881 gtgataccgg catgtgtcga atgtttgtac cctaagtctt tcatacggtc aagcatctta
    32941 gaagtttctt ttactttgaa tcgtttgaag acttctgcga tgatgtttcc taagtttttc
    33001 ttcttgaacg gtaatactaa ttcttgctct ttgatatgtg caggaatatc agtaccagct
    33061 tcaacaaagt atttgtccgg tgtttggacg gtcaagttga agtctgttgg ttcattcaga
    33121 taagggaatt caggaggcat gatctcgttg aagatgatct taccaactgt tgtgatcatc
    33181 gtacgttctt tttgccattc agtaaatggt ttttcaccta atgagttagg gttcacaccg
    33241 atacgtgaat gtaaatgaac atatccgttg cgccatgcga taacggcttc gttttgatct
    33301 ctgaagacca ttccttcgcc ttcgcgtcct tcttcttcca ttgtcaagta gtagttacct
    33361 agaaccatat cttgagaagg agtaacaact ggtttaccat ctttagggtt caagatgttt
    33421 tgagcagcta acatcaacat acgtgcttct gcttgtgctt cttcattcaa tggaacgtgg
    33481 acagccattt ggtctccatc gaaatcggca ttataagctt cacatacaag tggatgcaaa
    33541 cggattgcgc gtccttgtac taaaactggt tcgaatgctt ggataccaag tcggtgaagc
    33601 gtaggtgccc ggttcagcaa tactgggtgt tccttgatta cgtcttctaa aacatcccat
    33661 acttcgtctt cttgacgttc gattttacgt ttcgcatttt tgatgtttga agctagttcg
    33721 cgttgaacaa gctcacgcat cacgaatggt ttgaataatt caatggccat ttcttttgga
    33781 agtccacatt ggtacatctt caagaaaggt ccaacaacga taacggaacg acctgagtag
    33841 tcgacacgtt tacctagcaa gttttgacgg aaacgacctt gtttcccttt caacatatga
    33901 gaaagagatt tcaatgggcg gttacctggt ccagtaactg gacgtccgcg acgaccgtta
    33961 tcgatcaatg catctactgc ttcttgcagc atacgttttt cattttgaac gatgattcca
    34021 ggtgcattca agtctaacaa acgtttcaaa cggttattac ggttgattac acgacggtaa
    34081 agatcattca agtcacttgt tgcaaaacgt ccaccttcta attgaaccat tgggcgtaaa
    34141 tctggtggaa taacagggat aacgtccata accatccagc ttggttgatt tcctgaagcg
    34201 cggaatgctt ctaaaatatc caaacggcgg attgcacgtg tacgtttttg tccttgtgca
    34261 gatttcaatt cttctttcaa ttcggctgct tctgcatcta gatcaacatt atcaagtaat
    34321 tgtttaatcg cttctgcacc catagcagct tggaattctt gtccatattg ttcacgtttt
    34381 tcgcgatatt cgcgttctgt caacaattgt tttttctcta aattcgtatt tcctggttcg
    34441 atgactacat atgaagcaaa ataaatgatt tcttccaaag cgcgtgggct catgtctaat
    34501 acaagaccca tacgtgaagg aatcccttta aagtaccaga tatgtgaaac aggagctgct
    34561 aattcaatat gtcccatgcg ttcacggcga actttcgaac gagtaacttc cactccgcat
    34621 cgatcacaaa cgattccttt ataacggata cgtttgtatt ttccgcaggc acattcccag
    34681 tcttttgtag gaccaaaaat ccgctcacag aacaaccctt cgcgttcagg ttttaaggta
    34741 cgatagttaa tcgtctcagg tttttttact tcaccgtaag accagcttct gattttttcg
    34801 ggagaagcca aacctatttg catactttcg aatttattta catcgatcaa aaggggttcc
    34861 ctccatttca tttaatggca aaccgcaaga cggctgatct tcgaccgcct tgcagctcat
    34921 agaccggatt agtctaaatt atcttctgcg gtttcaaagt tttggatgac atcgtctttt
    34981 tcatcttcca cttgttcagc agctttcttt tctaattctt tggcagtttg ctgttcggca
    35041 aattttgtca aagcatcaac agtgatcaaa tcgtcgtctt catcgtccat gtcgcgaagt
    35101 tcgatctctg tttcttcgat atccaagaca cgcatgtcca gacctagtga ttgcaattct
    35161 ttcaccaata cgcggaatga ttccggtaca cctggttttg gaattggttc acccttgacg
    35221 attgcttcgt atgttttcac acgtccgact acgtcatctg atttgtatgt caagatttct
    35281 tgtaaagtat aagccgcacc gtaagcttcc agtgcccata cttccatttc accaaaacgt
    35341 tgtccaccaa attgagcttt acctcccaat ggttgttgtg taaccaatga gtaaggtccg
    35401 attgaacgtg catgcaattt gtcatcgacc atgtgagcaa gtttgatcat gtacatcaca
    35461 ccgacagaca cacgtccatc aaatggttca cctgtacgtc cgtcgtacag aacagttttc
    35521 gcatcgctgg ccataccggc ttcacgaact gtttcccata cgtcttcatc acttgcacca
    35581 tcaaatactg gcgttgctac gtggatacct aactgacgtg cagccatacc caagtgtaat
    35641 tccaatactt gcccgatgtt catacgtgaa ggcaccccta aaggattcag catgatatcg
    35701 attggtgttc catcaggtag gaatggcata tcttcttccg gcataatacg ggatacaacc
    35761 cctttatttc cgtgacgacc ggccatttta tccccttcat ggattttacg tttttgaacg
    35821 atataaacac gaactaacat gtttacgcct ggtgacaatt catctccagc ttcacgagta
    35881 aagattttca catcgtgaac gataccgccg ccgccatgtg gtacacgtaa tgatgtatcg
    35941 cggacttcac gagctttttc gccaaagatc gcatgcaata gacgttcttc tgcagataat
    36001 tctgttaccc cttttggcgt gactttccct acaagcaaat cgccatcttg gacttctgca
    36061 ccaatacgga tgatacccat ttcgtctaaa tcttttaatg cgtcttcccc gacattaggg
    36121 atttcgcgtg tgatttcttc aggtcctaat tttgtatcgc gggcttctga ttcatattct
    36181 tcaatatgga tagaagtgta tacgtcatct tttaccaaac gacggctcat gatgatcgca
    36241 tcttcatagt tgtacccttc ccaagtcatg aaaccaacta aaacgttttg gcctagagcc
    36301 atttcgcctt gttccataga tggtccgtct gccagagtat cccctttttc gactttctca
    36361 cccaaatgaa cgattggacg ttggttgtag cttgttccgg agtttgaacg acggaatttt
    36421 gttacatcgt atttatctaa tgcaccgttg tcgcggcgta cgcggatttc agaagcatca
    36481 acgtattcaa cgacaccatc atgtttacat aacaaagcag ctcctgagtc atgcgctgat
    36541 ttgtattcca taccagtacc aacccatggt gcttgaggat tgatcaacgg cactgcttga
    36601 cgctgcatgt tggcacccat caaggcacgg ttggagtcat cgttttccaa gaaaggaata
    36661 catgctgtcg caacggcaac tacttgttta ggtgatacgt ccatgtagtc tactttatca
    36721 atagatactt ctaagttttc actttgagca cgagccataa caacgtcttg agcaaatgtt
    36781 ccatcttcat tcaatgggct gtttgcttgg gctacgatgt aatggtcttc gatatcagct
    36841 gtcaagtagt cgatttggtc agtaacacga cctgtttcac ggtctacacg acgataaggt
    36901 gtttcgataa atccgaattt atttactttc gcataactgg acaaactatt gatcaacccg
    36961 atgtttgggc cttcaggagt ttcgatcgga cacatacggc catagtggga gtagtgaacg
    37021 tcacgaactt cataaccggc gcggtcacga gtcaatccgc caggtcctaa ggcgaaaaga
    37081 cgacgtttgt gtgtcaattc gccaagaggg tttgtttgat ccatgaactg tgacaactga
    37141 gaagaaccaa agaattcttt gatacttgcc acaactggac ggatattgat caattgttga
    37201 ggtgtcaatg tttcagtatc ttggatcgac attctttcac gaaccacacg ttccatacgt
    37261 gctagcccga tacggaattg gttttgtaaa agttcaccga ctgaacggat acgacgattt
    37321 cctaagtggt cgatatcatc gacattgccg ataccttcca tcaagttcaa gaaatagctc
    37381 attgaagcaa tgatgtcggc tggacgaacc gtttttacag ctgcttctgg atagccgttt
    37441 ccgatcacgt ttacttcacg ttcagggtct cttggtgaaa atactttgat gacttggaca
    37501 gtcattggat ctgtgaccac accgtcttct gatggatagt aagtcactga atttaggcca
    37561 ttatcaatga aaggagctag tgtttccatg acttggtgag tcagaactgt tcctttttca
    37621 acgatgatct cgcctgtttc tggatcaact aatgtttcag ctaatgtcag attcaataga
    37681 cgtgttttca aatctaattt tttgtttact ttgtaacgac caacgtttgc taagtcgtaa
    37741 cgttttggat cgaagaaacg agcattcagc aagttacgtg agctgtctgc tgtttttggt
    37801 tcgcctggac gcaaacgttc gtaaacgtct ttcaaacctt cttctgttct tgagtcgctt
    37861 gcgtttttat gcaagtcttt ttcgactgta ttgcgtaatg tttcgctgtc gccgaagatt
    37921 tcaaagatcg tatcatcgga accaaatcct aacgcacgaa ctaatacggt taaaggaatc
    37981 ttacgtgtac ggtcgatacg tacataagag atgtcttttg catcagtttc catttctagc
    38041 caagctccac ggttagggat aacagtagaa ccgaatcctt cttttccgtt tttgtcgact
    38101 tttccatgga aatagacacc tggtgaacgg actaactgag aaacgattac tcgctctgcc
    38161 ccgttgatga tgaatgttcc ttgttctgtc attaatggga aatcaccgaa gaatacttct
    38221 tgtgctttga tttcgccagt ctcgcggttt gtcaaacgca atgttacatg taatggtgca
    38281 gagtagtttg catcatgcgc acgagcttct gctactgtat actttggttc tttcaattca
    38341 taatctacaa attccaaaga tagattgcca ttgaagtcat cgattggcaa aatgtcttca
    38401 aacatttctc taagtccctc atctaagaac cattggtaag agtctgtttg aatttcgatc
    38461 aaatttggta attccaacac ttcactgatt cttgcgaaac ttctacgttc gcgatgtttt
    38521 ccgtatttta ctacgtgtcc agccaagctc ttcacccctc taaaatgtca ttcaaaaaga
    38581 ctacccgatc aattgttaca ataatatttc aaatattatt tttaggtaac aacagacatt
    38641 ttttgcggct tttaggcaaa aaaaaaccaa aaatagaaca ttctcttgaa accttacttc
    38701 gtgagcttct acttttgttt ccttatatta aagccgaaac ggacaatatt tcgtttctgc
    38761 cttttcttca tcagatagtt tttgcaactt catatactac acttattttc tataaaagtc
    38821 aacacttcct ggcgtttctt ctgctttttc gcaacatttt cttaatgttt tcaattttat
    38881 gttttcaatt atctgacaat tcttaattga cagacctttt tatctcggtt atactttaga
    38941 aaattcattt tgtggaggaa cttctatgcg aaccaacatc aaccaaatcg caccaacaca
    39001 actgaataac ttttacttta gctactttag ataggtttgt attcatcatg atggatacaa
    39061 acgttaaacg acagtttgta tctatgatgg ctaaagaact ttgtattgca gcaaaaagcc
    39121 acgtagacaa aatgctaagt ggctgagcag tgaaaataca aagtaaaagt atcaagcatt
    39181 ctactttttc aatttatttt ccgacttttc aatccatttg gacattttgt ctgaatggat
    39241 tttttgtttc ccaaaattaa ggaggaaaaa gaaatgaaaa aaaatataat catcggatta
    39301 ggcgctgccc tgctactagc tttaggaggt tgcgcacaaa aagacaattc cgcagaggca
    39361 tcagatggac aaaaaacaat tggcgtattg caacttgtac aacataactc gttagatgct
    39421 gcctataaag gattcaaaga aggattagca gaagaagggt ataaagaagg cgataatcta
    39481 acaatcgatt atcaaaatgc ccaaaacaat caagacaacc tgaaaagtat gagtgagaaa
    39541 ctcgtcaaag aagatcctga tttgctactt ggaatcgcca ctccagcagc acaatctt
//
LOCUS       DAZKKW010000017        38638 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.017, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000017 DAZKKW010000000
VERSION     DAZKKW010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 38638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38638
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.017"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(58..1197)
                     /locus_tag="ACG8PO_000876"
     CDS             complement(58..1197)
                     /locus_tag="ACG8PO_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFQ0206224.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAV
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(1506..2219)
                     /locus_tag="ACG8PO_000877"
     CDS             complement(1506..2219)
                     /locus_tag="ACG8PO_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HFQ0206225.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(2473..3174)
                     /gene="nagB"
                     /locus_tag="ACG8PO_000878"
     CDS             complement(2473..3174)
                     /gene="nagB"
                     /locus_tag="ACG8PO_000878"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFQ0206226.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            3408..4940
                     /locus_tag="ACG8PO_000879"
     CDS             3408..4940
                     /locus_tag="ACG8PO_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFQ0206227.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(5131..6333)
                     /locus_tag="ACG8PO_000880"
     CDS             complement(5131..6333)
                     /locus_tag="ACG8PO_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0206228.1"
                     /translation="MKSNEKKPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMAALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTVFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQTVVFKG
                     KRNLENKK"
     gene            complement(6415..7104)
                     /locus_tag="ACG8PO_000881"
     CDS             complement(6415..7104)
                     /locus_tag="ACG8PO_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFQ0206229.1"
                     /translation="MKKKIIRLILISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(7210..7578)
                     /gene="rplL"
                     /locus_tag="ACG8PO_000882"
     CDS             complement(7210..7578)
                     /gene="rplL"
                     /locus_tag="ACG8PO_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFQ0206230.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(7631..8134)
                     /gene="rplJ"
                     /locus_tag="ACG8PO_000883"
     CDS             complement(7631..8134)
                     /gene="rplJ"
                     /locus_tag="ACG8PO_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFQ0206231.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     misc_feature    complement(8338..8465)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(8544..9233)
                     /gene="rplA"
                     /locus_tag="ACG8PO_000884"
     CDS             complement(8544..9233)
                     /gene="rplA"
                     /locus_tag="ACG8PO_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFQ0206232.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(9337..9759)
                     /gene="rplK"
                     /locus_tag="ACG8PO_000885"
     CDS             complement(9337..9759)
                     /gene="rplK"
                     /locus_tag="ACG8PO_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFQ0206233.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            10102..10770
                     /gene="sdaAB"
                     /locus_tag="ACG8PO_000886"
     CDS             10102..10770
                     /gene="sdaAB"
                     /locus_tag="ACG8PO_000886"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HFQ0206234.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            10783..11655
                     /gene="sdaAA"
                     /locus_tag="ACG8PO_000887"
     CDS             10783..11655
                     /gene="sdaAA"
                     /locus_tag="ACG8PO_000887"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HFQ0206235.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(11746..12846)
                     /locus_tag="ACG8PO_000888"
                     /pseudo
     CDS             complement(11746..12846)
                     /locus_tag="ACG8PO_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324221.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
     gene            complement(12938..13825)
                     /locus_tag="ACG8PO_000889"
     CDS             complement(12938..13825)
                     /locus_tag="ACG8PO_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFQ0206236.1"
                     /translation="MTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEVSDKEKT
                     AESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDGQIQATG
                     DLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSMGGVSAL
                     RYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQDYQQMI
                     GNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTGENASHS
                     MLHENQEVDQLVSRFIWKE"
     gene            complement(13902..14447)
                     /gene="nusG"
                     /locus_tag="ACG8PO_000890"
     CDS             complement(13902..14447)
                     /gene="nusG"
                     /locus_tag="ACG8PO_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFQ0206237.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(14548..14718)
                     /gene="secE"
                     /locus_tag="ACG8PO_000891"
     CDS             complement(14548..14718)
                     /gene="secE"
                     /locus_tag="ACG8PO_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFQ0206238.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(14742..14894)
                     /gene="rpmG"
                     /locus_tag="ACG8PO_000892"
     CDS             complement(14742..14894)
                     /gene="rpmG"
                     /locus_tag="ACG8PO_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFQ0206239.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            15056..15700
                     /gene="cbpA"
                     /locus_tag="ACG8PO_000893"
     CDS             15056..15700
                     /gene="cbpA"
                     /locus_tag="ACG8PO_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-AMP binding protein CbpA"
                     /protein_id="HFQ0206240.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(15756..16259)
                     /locus_tag="ACG8PO_000894"
     CDS             complement(15756..16259)
                     /locus_tag="ACG8PO_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HFQ0206241.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(16272..16318)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            16856..17821
                     /locus_tag="ACG8PO_000895"
     CDS             16856..17821
                     /locus_tag="ACG8PO_000895"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFQ0206242.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGDDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(17920..19092)
                     /locus_tag="ACG8PO_000896"
     CDS             complement(17920..19092)
                     /locus_tag="ACG8PO_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0206243.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMPTIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(19079..20224)
                     /locus_tag="ACG8PO_000897"
     CDS             complement(19079..20224)
                     /locus_tag="ACG8PO_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0206244.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(20238..21162)
                     /locus_tag="ACG8PO_000898"
                     /pseudo
     CDS             complement(20238..21162)
                     /locus_tag="ACG8PO_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            21279..22358
                     /locus_tag="ACG8PO_000899"
     CDS             21279..22358
                     /locus_tag="ACG8PO_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFQ0206245.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            22355..22983
                     /locus_tag="ACG8PO_000900"
                     /pseudo
     CDS             22355..22983
                     /locus_tag="ACG8PO_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325326.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(23093..23320)
                     /locus_tag="ACG8PO_000901"
     CDS             complement(23093..23320)
                     /locus_tag="ACG8PO_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFQ0206246.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(23423..26011)
                     /locus_tag="ACG8PO_000902"
     CDS             complement(23423..26011)
                     /locus_tag="ACG8PO_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0043139 - 5'-3' DNA helicase activity
                     [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HFQ0206247.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(26031..26690)
                     /locus_tag="ACG8PO_000903"
     CDS             complement(26031..26690)
                     /locus_tag="ACG8PO_000903"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFQ0206248.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVAQHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(26739..27242)
                     /gene="trmL"
                     /locus_tag="ACG8PO_000904"
     CDS             complement(26739..27242)
                     /gene="trmL"
                     /locus_tag="ACG8PO_000904"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFQ0206249.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(27463..28329)
                     /locus_tag="ACG8PO_000905"
     CDS             complement(27463..28329)
                     /locus_tag="ACG8PO_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFQ0206250.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            28438..29079
                     /locus_tag="ACG8PO_000906"
     CDS             28438..29079
                     /locus_tag="ACG8PO_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HFQ0206251.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(29141..30502)
                     /gene="mgtE"
                     /locus_tag="ACG8PO_000907"
     CDS             complement(29141..30502)
                     /gene="mgtE"
                     /locus_tag="ACG8PO_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HFQ0206252.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(30520..31419)
                     /locus_tag="ACG8PO_000908"
     CDS             complement(30520..31419)
                     /locus_tag="ACG8PO_000908"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFQ0206253.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(31421..32218)
                     /locus_tag="ACG8PO_000909"
     CDS             complement(31421..32218)
                     /locus_tag="ACG8PO_000909"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HFQ0206254.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(32196..32900)
                     /locus_tag="ACG8PO_000910"
     CDS             complement(32196..32900)
                     /locus_tag="ACG8PO_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HFQ0206255.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            33031..33606
                     /locus_tag="ACG8PO_000911"
     CDS             33031..33606
                     /locus_tag="ACG8PO_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HFQ0206256.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            33724..34401
                     /locus_tag="ACG8PO_000912"
     CDS             33724..34401
                     /locus_tag="ACG8PO_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP adapter SpxH family protein"
                     /protein_id="HFQ0206257.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNLLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
     gene            complement(34480..36288)
                     /gene="pepF"
                     /locus_tag="ACG8PO_000913"
     CDS             complement(34480..36288)
                     /gene="pepF"
                     /locus_tag="ACG8PO_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HFQ0206258.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSDAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(36464..37462)
                     /locus_tag="ACG8PO_000914"
     CDS             complement(36464..37462)
                     /locus_tag="ACG8PO_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA family protein"
                     /protein_id="HFQ0206259.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(37688..38341)
                     /locus_tag="ACG8PO_000915"
     CDS             complement(37688..38341)
                     /locus_tag="ACG8PO_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HFQ0206260.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
BASE COUNT        11541 a         8139 c         6783 g        12175 t
ORIGIN      
        1 aataaaaaga gtttagcgta cagatatttt aaatgtacac tgaactcttt tttatgctta
       61 ttcacttgtt tcttccagca cttttggact gtctaacttt tccgaaacat gttccattct
      121 tctcaataaa tagattttcc ggatatttaa tattatcgtc ttaatcactg catagacagg
      181 tatgcaaatc agcatgccta atattcctgc gacacttcct gctccaatca ataccaagat
      241 gatcgtcaaa ggatggatat tcattgattt cccaaataac aacggcttga tcaaatttcc
      301 atctaattgt tggatcacta aaatcgacaa cgccatatac aaagcttgaa atggagagat
      361 aaacaaaccg acaatgattg ctggtaccgc tccaataaaa gggccgacat acgggataat
      421 attcgtgatt ccgcaaatga ccgcaagcaa caagctataa ggctgatcga atattctcat
      481 tgccaaaaag ctcattacac caataatcaa agcatctaag atcgtactgc tgatataggc
      541 agataatgtt tcgtttaatt cttttaccgt ttggcgtaac tctttacgaa tcgctttcgg
      601 aaagaagtga gacaacgcat caataaattt gtgcccatcc ttgaacataa atagcaaaat
      661 aaatggaacg gtaaataaca aaataaaaaa ttgcatcata aatgaaatga tttttgagac
      721 acttgcagat aaactgacta atatgatatt gaacaaatta ctaagagata aatttgctga
      781 tgctaattgt tcttccaaat tgaaattttt aaactcttcc cgctgcgcca attcatttaa
      841 ccatttgctt gtttcatctg catacattgg caagctctgg gtcaattgaa ttgtctgttc
      901 cattaactga ggcactacat tcatgacagt taaaacaact aaaatgatta cgaccaaaaa
      961 cgtcaataaa aaaccgaaca ctctcgggat ccgatgcttt tcgagaaaaa caacgattgg
     1021 atcgaacata taatagataa aaccagcaac aagcaatggt aaaaaaactg ccgaaagcat
     1081 tttcaatatt ggactaaaaa tattcggcat ttgtaaaata aagtacacgc cgatgatcgt
     1141 gagtactagc tccactgtcc agaaaaataa ttttgattgt ttaaaacgtg attgcataca
     1201 tctcctccaa ggtcctttga tacttctatt ctatcattta acaaagagtt acgcgctgag
     1261 attttcattt gcttaaaaaa aacaaaaaaa ataaacatat tttaatgagt agacgagtac
     1321 ttaaacaaaa acacggtttg gagtagtgac aaaaattata ctgcagtccc tttttccaaa
     1381 taaacggtgt tcaaaagctt gcatcttcgt aataagccca gccaaacgaa aaatataagg
     1441 aactattttt atttggctgg gcttattact tgatgtagga catctatttt acaacctcag
     1501 atcatttatt gcgcaatact gactaaatgc aactttgtga tatttttgtg tacggacaac
     1561 tcctcgatca catcggcata agtgagccct tttggaagat caatcgtata gatatttttg
     1621 tacactcggt agtcatcaac gaattttacg tcaaaattca catcttcgat tacgatattt
     1681 ttctcttcaa aataatcggt aagaaaatct ttggtttctt ttctatggat aaattggatt
     1741 tctaactttt tagtcgtagg tacatggatc acgcgtttca ctactgtgag tgcaatagca
     1801 atcccgataa aactgatgat cgcaatggaa tagtaaccca tgccgatggc gatccctaat
     1861 ccagcaattg cccataaaga agcggcagtc gtaagtcccg tcaccgattg tttcgtgacg
     1921 atgatcgttc ccgcgcctaa gaaaccgaca ccactcacca cttgagcaat cagccttgct
     1981 tcatctgaac gaatcgttcc agcaagctgt ggatactcta aagcatctct taatgcacca
     2041 ctagcgatct caacttggat caatgcaatg atacatgctc ccatacaaac taaaatatgc
     2101 gtacgcatac ctgctggacg atttttatat tgtcgttcaa aaccgattgc tccgccaaat
     2161 agcatagcga tccctaaacg taaaataatc tctggaattg tcaataccat atcattcatc
     2221 taaacaccct ccagtccaat atatcatgtc tgaaaggagc agacaaaaaa agtgcaattt
     2281 ttacctctta cgaaaaaagg actgtgacaa aatttttgtc atagtccttt ttatgaataa
     2341 acggtgttca atcagctgac cctcggtatt actcaacaaa cggcaaaata tggggagcta
     2401 tttttccttt gtcttctcta atactcaggt caaaacgctg atttcacaac ctctttagcc
     2461 tactattgaa aattacaact tacttgctgc ttcttcatcg atgatcacaa caacattgtc
     2521 gtgattttgc aatacacttg ctggcaaatg attcgttaca ggtccctcaa tcatcccttt
     2581 gattgcatca gctttgcttt cgccaaatgc catcaagacg atttctttac ttttcataat
     2641 tgaaccgatt cccattgaaa cagcttttgt tggtacatct tctgctttat cgaagaatcg
     2701 tttgtttgca ttgattgttg attctgttaa gtcaactact gaagtcgttc cgtcaaatga
     2761 agcaccaggt tcattgaaac cgatatgtcc attttgcccg attcctaaga tttggatatc
     2821 gattggatgt gcatcgatga tttgatcata ctttttgcat gctgcttcca gatcttctgc
     2881 ttttccatct ggtacaaacg tttctttgaa tggtttttta tcaaataatt ggacatccat
     2941 gaaatgacgg tagctttgtt catctgaacc gcctagtcca acgtactcat ctaagtttac
     3001 agaagtcatt tcactgaaat ccaaatcgct tgaagtcatt tctttatata aagtaactgg
     3061 tgtgcttcct gttgccaatc ctaatacttt tgcacctttg ttcatacctt ctttgatgat
     3121 ttcaaatgct ttttttccgc cttcttcagc gttttttaca cgaatgattt ccatagtgat
     3181 tgctccttta tattttggta tagcccaatt atagcattct ttttttcttt ttacaataat
     3241 tagtatagac caaattgcat ttttttcatt ttcttgacaa taaataggaa tccgtgttag
     3301 gtttcataag atggttcaat caaacaatcc atgttgcatg aaaaccaagc aagtgattca
     3361 ctgtggacgc tttatgtctg cagttttttt atgaaaaagg agttattatg agtatactga
     3421 cattaaacaa cattacccag caatttggcg acaaaatcct ctatgaaggt gtcgatctgc
     3481 aattaaatgc tggcgaacat cttgggctga ttggacaaaa cggagccggt aaaagtacat
     3541 tgattaaaat catcactggt gagatcttac cagatgacgg acagatccat tggcaaaaaa
     3601 atattcacaa aggttacctc gatcaatatg tggaagtcga tgaaacatta acaatcgaag
     3661 aatttctaaa aactgcgtac gcaaaagaat acgaaaagga agaaaaaatt gcttttcttt
     3721 accaaacata tagcgagacc ttcgatgatt ccttgctgga aaaagctggg aaactccaaa
     3781 cggaattaga ccaaggagtg ttttatcaaa tcgacacctt agttgctgag atgagcagcg
     3841 ggttggggat cgatgtctta ggtttagatt cactgttgaa aaatctaagt ggtggacaac
     3901 gttcaaaggt gattttagca aaactacttt tagaaaaccc agatgttctg atcttagatg
     3961 aaccaaccaa tcacttagat gatcagcata tccaatggct gacgcagttt cttcaggatt
     4021 ttgacggtgc gtatatcgtg atttcccatg accaagaatt tcttgatcag atcaccaccc
     4081 atatcgcaga catcgagttt ggaaagatta ctaagtatac tggccatcta aaacaagcat
     4141 tgaaacaaaa ggaacaaaat cgcgaaagct atttgcgcca atatcatgct caacaaaaac
     4201 aaatcgaaaa gaccgaagct tatattcgaa aatacaaagc cggttcccga tcaacgatgg
     4261 caaaaagccg acaaaaacaa ttagataaaa tcgaacggct gacgcctcca gcttcagcaa
     4321 gtaaagctgt ctttgatttt ccattctcgc ctattgtcac gacattagca gtggaaacaa
     4381 ctgatttagt catcggatat gatcgaccgc tacttgcacc catcaatttg accatccgct
     4441 ttggtgaaaa tattgccatt cgaggcttca acgggattgg aaaatctact ttattaaaaa
     4501 cactgatcgg tgagattgaa aaaataagtg gcgatttcca cttcccagat aacacaaaaa
     4561 tcaattactt ttcacaagat ctgtactggg aaaatccatt agaaacacca ctacaatatt
     4621 taagcagtca atttccaaaa gcgactatca aagaattacg tcgccaacta gctaaagcag
     4681 ggttagttaa tcaattagca agtgaacctc tcactacatt aagtggtggg gaacaaacta
     4741 aggtgaaact tgcacagttg acaatgaacc aaggtaattt actgattctc gatgaaccga
     4801 cgaaccatat cgatcaagaa acgaaagaaa gcttacaaga cgggatacag aaatttccag
     4861 gaacagtaat catcgtctct catgaacaag aattttatca agatcttgtg caacgagtca
     4921 ttgagattga accaaaataa acgtttgatt gaatagttga aaaaaaggag ctgtgacaaa
     4981 agttttgtca caactccttc ttctgaataa atggtggcca attagctgac cttcggtatt
     5041 agctcaaaaa acggcaaaat atgaggagct atttttattt ggctattctt attacttgat
     5101 gcagaacagc ttttcacaac ctcttttcgt ctatttttta ttttcgagat ttctctttcc
     5161 tttaaaaaca actgtttgaa agaagaacgc acatccttga aaaacaagcc cgctaaccgt
     5221 tacacctaac cagccatact gctgccacat tgtcgtccct agcaatgaac cagccgcacc
     5281 tccaataaag tacataaaca taaatacagt attatttcgg ttgcttgctg cttcacctag
     5341 cccttgtact cgtgtttgat tggcgacttg accaaattga ttcccgacgt ctaataaaat
     5401 aatagctaaa agcaaaatgg ccaaatggct tccgccaacc aataaagcaa taaagcttat
     5461 tgtttgcata acaagtccca tatacacgat ttttctttca gaataagaat ctgccagtcg
     5521 tcccacgata ggtgcagcaa ctgctccaga caaaccaaaa atcgctaata tccctgcttc
     5581 gaatgttccc caattgtagg cgggactgct gatataaaaa atcaacgtag accaaaagat
     5641 cgaaaatgtt ccaaacatga aaaatccact gactgccgct tctcttaata gacgttgaga
     5701 agcaactaaa tgcggcaaac ttttaagtga atcaaggtat gaaacagctg tactactttg
     5761 ctggacagaa cgcggcattt tccagtaaag cagaagtgta agaattccga ctgcacataa
     5821 agcaaataaa tagatcatcc gccaagaaaa aagactggca acaataccag ataccgtccg
     5881 agaaagcaaa atccccacga gtaaaccgct aagcaatgta cccatgacct tccctcttgc
     5941 ttttggtccc gctagtaccg caccgtaagg aatgatgatt tgcggcacga tcgaaagtaa
     6001 tcctattaaa aaagaagcgc atgcaaataa cgtaaacgaa ggcgcaaaaa aagcggccaa
     6061 cagagagacg gcagataacg ctgccattcg aatgatcaat tttggacgat ccaccacgtc
     6121 ccctaaagga actaaaaaca gtaaccctaa cgcatagcct aattgggtca acatcgtcaa
     6181 tatcccgact gcacttgtgc taacagataa actagctgct acttttgttc caatcggctg
     6241 gatgtaatac atattggcaa caacaacgcc acaagtgatt gataatagta aaataagcga
     6301 agttgttaaa ggtttttttt cattactctt cattgatttc ccccatttcc tatcttagtt
     6361 tacaacttat ggaaaagatt ggaaatagaa aaatacaact attcgccaca cttattattt
     6421 gtgcttttgt tttccttgtt ttttcttgcg atctttataa atgaagacaa ctaaccctac
     6481 tagcacagta acacttgcaa ccaacgtgac tgcagttttg ttatatccgt gaaacagagc
     6541 ttctccacca cacgcataca acacagacga agggatgact ccaatcacga tcgacaatat
     6601 gatttgctgt agaggcaatt tcatcgcatc tgcagcaaaa ttgaccatag aagatggaat
     6661 aaccggtatc atatagccta acattacacc tatttttgga tgtttcattt gtcggattgc
     6721 ttgcacccaa tgattcgttt cggttttctt atctaaaaat tttaagcgat gcataaggaa
     6781 aattgcagtg atgttaccaa gagcattacc caaaatgttg atgatcgcac ctgtcagtgg
     6841 tccaaagctc aaaccagcaa tgactccaac tactgaagta ggaatacctg gaacagcaca
     6901 catcaccgct gtcaatacga tcaagagtat cgatgcaaaa gtcccatgcg aacgaatatg
     6961 tgcgagtagc tctgccttac tcgctctcgg atttaaaaat aattggatgt ctgtctgata
     7021 ttctaaatac aaccgatagc ccactaccag tatcagaatg attcccaacg agatcaatat
     7081 caatcggatt attttctttt tcatatttcc tcctgaaaaa aatctatatt cattaaataa
     7141 tagtttaaca taaaaacaaa aaagcaccga aatccaacat aggatctcag tgcttttaaa
     7201 tagtaaaaat tatttaacag ttacagatgc gccaacttct tcaagtttag ctttcaattc
     7261 ttcagcttct tctttagata cgccttcttt aactggtgca ggagcgccat caactacagc
     7321 tttagcttct ttcaagccta aaccagttgc ttcacgaact gctttgataa ctttaacttt
     7381 ttggtctcca gctgcagtta attctacagt gaattctgtt tgttcttcag cagcagcagc
     7441 gccaccagca gcagctacag ctacaggagc agcagcagat acgccaaatt cttcttcaat
     7501 agctttaact aggtcgttaa gttctaggat agttgcttct tttaactcag caacgatgtt
     7561 ttcaatgttc aatgccatta taatttcctc cgtttaattg ttctttttta tttaatgctt
     7621 tatgcggcaa ttaagctgca tctccatctt ctttgtctgc cactgctttg acagcgtaag
     7681 ccacgttgcg gactggcgct tgtagtacag atagcaacat agatagaagt ccttcgcgat
     7741 ttggcaattt tgccaatgct gtgatttctt ccaatgaaga gactttacct tcgatgatac
     7801 cgcctttgat ttctaatgct tttgcttctt tagcaaattc gtccatgatt ttcgctggtg
     7861 cgactacatc ttcgttactg aatgcaacag ctgtaggtcc agtgaatact tcgtccatgc
     7921 cttcaaggcc agctttttta gctgcacgtg aaagaataga atttttgata actttcattt
     7981 ctacgttagc atcacgaagt tgtttacgta aacgtgttac ttcatcaact gttaatccgc
     8041 ggtagtcaac gactactact gatgcagctg ctgtaaattt ttcgcttact tcatcaacga
     8101 ttgctgcttt ttttgcgata attgcttcac tcatttttga gtttcacctc ctgaattttg
     8161 atggaagagt tttttgcact gaaaaggcaa gtcactttta tagttacttt gctcaaccag
     8221 tattattcat cacctattca cttcgttcat agtgattcta ctcgctttgc tcgaaaggaa
     8281 actcctttcg ctttgctcaa ccagtattgt tcatcatctc ttcacttcgt tcatagtgat
     8341 tcacaataaa aaactctatg ccaccgcaga catagaggga ctattgaata tattcaataa
     8401 acgtcctcgg taggaaatta aggcttgatg ccacctactg tcttcggtac aagttaatta
     8461 ttaacctcaa ctagcttagc atgctaaact agttgagtca agaattattt ttcagttgga
     8521 tcaatcaaca ttctaatatt taattagaaa gaagcttggt caacatggat accaggtcca
     8581 aatgttgttg tgatcgttac atttttcatg tattgacctt tagctgctga tggtttagct
     8641 tttagcaata catcatggat tgctgcaaag ttttcaacta atttttcatc gtcaaatgaa
     8701 acttttccga ttggtacatg gatatttcca gctttgtcca cacggtaagt tactttacca
     8761 gctttcactt cgtttactgc ttttgttacg tccattgtaa ctgtaccagt tttagggttt
     8821 ggcattaagc cttttggtcc taatactcgt cctagacgac ctacagtagc catcatatca
     8881 ggtgttgcaa caactacatc aaagtcaaac catccgtttt ggattttttg aaccatgtcg
     8941 tcgtcaccta cgaaatctgc accagctgct tcagcttctt ttgctttgtc tcctttagca
     9001 aaaactaaaa cagtttgtgt tttaccagtt ccgtttggta atacaacagc accacggatt
     9061 tgttggtctg cttttttagg gtctacgttc aaacggtatg caacttcaac tgttgcatca
     9121 aattttgcaa tgtttgtttc ttttgctaaa gctactgctt cggcaacagg ataaacttta
     9181 cttgaatcaa ctttttttaa tgcatcttgc atttttttgc tctttttagc cattttggtt
     9241 cctccttgat tgtggttata acggttgtac ctcccacgtg ttctatatct ttcaatatag
     9301 agccaactga gaagctgctg tcttagggtg tatagattat tctacagtga tccccatgct
     9361 tcgtgcagtt ccttcaacca tgcgcattgc tgcttcaacg ttagctgcgt ttaggtcttc
     9421 catttttaat tcagcgattt ctcttacttg atcgctagaa actttcgcaa ctttattttt
     9481 gtttggttcg cctgaacctt tttcgatttt agctgctttt ttcaatagaa cagcagctgg
     9541 cggtgttttt gtaacgaatg tgaatgaacg gtcttcgtat acagaaataa caaccggaat
     9601 gatcaaacct gcttgatcag ctgtacgagc gttgaattct tttgtgaatc ccatgatatt
     9661 gatacccgct tgacctagtg caggacctac tggtggagct ggagttgctt tacctgcagg
     9721 aatttgcaat ttaacgattt tttctacttt ctttgccacg agacatacct ccttaagtcc
     9781 gtgatgtggt taattggaga tgcatatttc tcctcccacg ttttatctga tctgaaaaaa
     9841 tccagacaaa taatgtgcaa aacaatgcac actggtacag tatagcaaat ttatttgttt
     9901 tttcaagatt tttttctgct atttttacat tttgcttgaa taaacgctaa atttattcta
     9961 aaaaaacaac aatttttcgt tctcatttct tcttttatgt tacacttgcg ctagcatggt
    10021 tcaacagatg aaagcaaaca ataacacggt tttttagacc cttgtacaaa gattcctaca
    10081 taacaaaatg aggtggagaa aatgaaagat ttaaggtata gaagtgtctt tgatattatt
    10141 ggacctgtga tgattggtcc aagtagctct cacactgccg gagctgctag aatcgggaaa
    10201 atcatacgta gtatatttgg cgaacagcct gattctgtag atatttatct atatgaatca
    10261 tttgcaaaaa cctatcgggg gcacggaaca gatattgcat tagttggcgg cctgttaggg
    10321 atggaacccg atgatgaacg tttagctgat tctttgaaat tggcatatga agccggtatg
    10381 gaagtcttgt ttgttcctaa gagtgaaaaa gccgaacatc caaactctgt aaagatgctt
    10441 ctaaaaaaag gaacacgaaa gctttcagtc actggcattt cgatcggcgg tggcaatatc
    10501 cagatttctg aattgaatgg attcaaaatt tctttaagca tgggtactcc taccttgatc
    10561 gttgtccacc aagatgttcc aggtatgatt gctaaagtga ctagtatact ttctgagacc
    10621 aatacgaata tcggcactat gacagtgaca cgggaatcaa aaggtgaaaa tgccattatg
    10681 ataattgaag tagatgatcc tcaagtagaa gaaacggtaa aaagactcaa acagctgcca
    10741 aatatcgaca gcgtaaatta tttcgattaa ggagctaaaa aaatgttttt atcgattgaa
    10801 gaattagtag atcaagcaaa agcttatcca agtgtagcag aattgatgat tgcagttgaa
    10861 atggaaatga gtggccgttc ccgtagtcac atcatcgaga tcatggaaaa aaacctctca
    10921 gtaatggaac aatcaatcaa cgagggaaaa gaaggtgtta cctccgtcac aggaattacc
    10981 ggtggtgatg caaaaaaatt agatgattat ttaaatgagg cagacttttt aagtggagaa
    11041 acaatcttaa aagctgtacg caatgctgta gctgttaatg aagtcaatgc aaaaatgggg
    11101 ttgatttgtg ctacaccgac tgccggaagc gcgggtgttg ttcctggtgt gctgatggct
    11161 gtccgagatc ggcttgattt gacacatgaa cagcaattgg aattcttatt taccgctggc
    11221 gctttcgggt tagtcatcgc aaacaacgct tctatcagtg gtgcagaagg gggatgtcaa
    11281 gcagaagttg gttctgcaag tgcaatggca gcagcagctt tggtttgtgc aaaaaatggg
    11341 actcctcatc aagcagcaca agcagtagcc attacattga aaaatatgat gggactgatt
    11401 tgcgatcccg ttgcaggctt agttgaagtc ccgtgtgtca aacgtaatgc tttaggctct
    11461 tctcaagctt ttatctctgc tgatatggca ttagcgggta tcgaaagtgt cattccgccg
    11521 gatgaagtcg ttgctgcaat gtaccaagtc ggacgtcaaa tgccgtcgat tttcaaagaa
    11581 acagcagaag gtggcttagc tgttacgcca actgctaaac gacttgcgga agagatttta
    11641 aataagaggg cgtgataaag ctccttaatt tcgagtatta agagattagt tatcttcata
    11701 acaaaaaacg aagagaggac taattgccct tctcttcgtt ttttattaat ccatcaattt
    11761 cgtatttttt acttcatcga tcgtttttgt tcctgcaagt tgcatcgtga tcgacaattc
    11821 tttgttcagg tgttcaaaaa cagatgttac tccttctgcc ccacctagat tcaacccata
    11881 aatcactggt cggccaatcg caactaagtc agcaccactt gccagagctt tgaagacatg
    11941 ctctcctcga cggacaccag agtcgaaaat cactggtaca cgtttgttga ctacttcggc
    12001 aatttttggc aatacttcaa aggacgcagg acctccatct agttgacgtc caccatgatt
    12061 ggatacccag ataccatctg cccctgcaga gatagcgatt actgcatctt ctggcgattg
    12121 tattcctttt acgataactg gaagatgggt gatttctttg attgttttga tgtcatctgg
    12181 tgtcaagcct tgttttgcag ctgcataaat ttctgcgatt ccttttcctt cgccattacc
    12241 acttgcactt tgttcgctat acgcagctaa gttaggcatt ggtaatggaa attggaattg
    12301 attgatgacg tcttcttcac ggtaaccacc gagcgttgaa tctgccgtta aaatgatagc
    12361 tttagcccct gcttctactg ctttgttcaa aatgaactca ttgaatctgt cgtctttact
    12421 catatataat tggaaaaact gcggtgcgcc tggcgctgca tcggctgcgt ctttgattgt
    12481 cgtatttgca taagtgctga tggaaaagat tgatccagca gcagctactc ctttagctgt
    12541 atcagcttcc cctttttcat gtgccaatcc ttgggccgct gatggcgctt ggataatcgg
    12601 tgtatctaat tcaattccga aaacagatgt atgcaaatcg gctgagtcga ttccgcgtaa
    12661 tatccgtggc atgattttct ttgtattaaa agaagtcgta ttttctttca tcgtccactc
    12721 atcttcagaa ccaccgcgaa tatagccaaa agctcctgct tccatgcgct cttttacacg
    12781 accttctagt taggcaatgt ttacaatatc gattggatgt tcttcattac ttgtatgata
    12841 agtcatagta gatccttctt tcatcttctt gattacatat aaatcatact aaacttacaa
    12901 aaagtaagca aacatttact ctcagatact tttatcgcta ttctttccaa ataaatcgac
    12961 ttacaagctg atctacttct tggttttcat gaagcatact atgagaggca ttttcacctg
    13021 tcactacttt ttcctctatt tcatttccgc gttgcttcaa caacgcatag accgctaatg
    13081 cactgtttaa cggtactgtt ccatcactca agtctgtttc atctagttga ccagcaatca
    13141 agaaaaatcg agtggttata ggaatattac cgatcatttg ctggtaatct tgatagcggt
    13201 tagaaacgac agcaggcccc tttgctaact catctgtcag agactgcgca ctatcatcga
    13261 caaaatcatt aaaaggcgcg ccaattgctg ttaatgtctg aacttgtggc aatgacgtat
    13321 cctgtccata cgttcctaaa tagcgtagtg cactaactcc tcccatggaa tgccccacta
    13381 tgttgacttt atctatgccg tagttttctt tcagatacaa aagacaagtt ttgatccatt
    13441 ctgcttgatt ccactcgtta tttttattat cttcaaacaa tacttggacc atcggattgt
    13501 ctgcttttcc agacaaatct cctgttgctt gtatttgtcc atctgttcct actgttaaaa
    13561 ccaattcttt tactgcttgc tgattttttt ccaaacgccg caacatccct ttaaaggaat
    13621 tttctgtccc gctgtagccg tggaaaaaaa gtgttggaat agtggttttt tctatcactt
    13681 tactggattc tgccgttttt tctttatcag aaacttcagt agtatttatc ttcgtcgtct
    13741 ggcaaccact taacgcaaac aaactgataa ggactaaagc tgctgcagtt gcacgtttgc
    13801 ataaattttt gattggttct gtcatacacc tcttcctttc catatagaaa aaagaaagac
    13861 ttgaaaggcc aatcatatga tcgacatttc aagtctcttt attaatcgat tttatctact
    13921 tgatcgaaat caagttctgt actggtttca cgtccaaaca tatcgatgtt gactttcaat
    13981 ttttcttttt ctccgtcaac ttctgttact tgtccttcta atccagcaaa tgcgccttcg
    14041 atgattttca cgacttcgcc aacttctact tctagatcat tttgtcgcag gctcataccg
    14101 atagaacgca agatatgatt gacttcttct ggaagtaatg gtgctggttt gcttccggct
    14161 ccgtgtgaac caacgaatcc tgtcacacca ggtgtgttgc ggacaacata ccatgaagcg
    14221 tctgtcatga tcatttcaac aagtacgtat cctgggaatg ttttatgaac gatttctttt
    14281 tctttcccat ttttgacttc agtttctttt tcttcaggaa caacgacacg gaaaataaag
    14341 tcggccattt tcatactttg cgcacgagat tcgatatttg cttttacttt gttttcatat
    14401 cctgaatatg tatgcaagac ataccagttt ttttcaaaag attccattga aggctcctca
    14461 tttcattgta aaataaaaaa acctcagtac acccgaagtt tttctttcgc ttcattatag
    14521 cactaaaagt acgttgtggc tagcgagtta ttttaaaatc cagccaaata agctttggat
    14581 ggctgtatcc attacgtaaa acatcaatcc gaaaataata gaagtttcga tgactactaa
    14641 cgtatcttta cggagttgtt ttctgcttgg ccaagttact tgtttgattt catcttttac
    14701 gctttttaga aatttcatga gacaacctcc cttgctactg tttatttcgt ttctttatgt
    14761 aatgtatatt gattacaata tttacaaaac ttattgattt ccaaacgttc gccgcttttt
    14821 ccttcactga ctgattttgt gtagttgcga gacccacaaa cggaacaagc gagtgctgct
    14881 tttttctttg ccatttactt ctgccccctt gctgagaatc agacttatac ttcataacag
    14941 tatcacgtaa ctttttactt gtcaatttgt gaaataaact atctattgtt ttggataaaa
    15001 ttatgatatg attaatttga aattaagcta aaatgagaca attggaggta tagcgatgct
    15061 attaaaaaca cttgtatata agaaaaaaga tctgacaacc gttacagaga ctagtacatt
    15121 agaagaagca ttaaaaattt tagaaacttc cggataccgt tgtgtgccga tcttagattc
    15181 ttcaggtaat attttccgtg gaaacattta caaaatgcat atctatcgtc ataaagcaaa
    15241 tggcggtgat atgacgcttc ctgtcactca tcttttgaaa aacgctacaa agttcattta
    15301 cctggatact tctttcttca aagtattctt tacgatcaaa gagcttcctt atattgctgt
    15361 ccttgacagt gaaaatcatt tttatggtat tttgacacat agtacgttgc ttaatatgct
    15421 ctctcaatct tggagcgtag aacaaggaag ctatgtactg acaattgctt ccacaggaaa
    15481 gcaaggagat ttggctgcta ttgcaaaaat catttcaaaa cacagcagca ttgccagctg
    15541 tattactctt gatatcgaaa aagacgaata cattcgacgt acattgatca cgctaccggc
    15601 taacactgaa gcgaaagtat gtgaaacgat cgtcaaccac ttagagaaaa agaattataa
    15661 agtggtagaa attgaaaatt tgcaaaacca agaagcataa tcaaaaagcg aaaaaagaaa
    15721 acagctaatc tgctttcttt tttcgctttt tataattaga cacgcttttc aaaatcaagc
    15781 gcgcgatcta aataatacat caatggtgct gaaacagcca tgacgataaa ctcacttaat
    15841 gcaagcgtgc cataggttgt ccaaaatgct tggccacctg ttggaactaa catataagca
    15901 atcagtccca tgctgatggt aaagaagatc gtgttcaata ctaaacgcat ctttacattt
    15961 ttcactttgt tttgcaataa agctgtcagg cctaaagaaa gcaatgtttg gccgccacca
    16021 aaaaccacat ccatgatacc aaaaccaaag aaggcattat agacgatgac cccgcctaaa
    16081 actccccaaa gcaatttacg gttaaagaca actaaatgat tcaaactttc tgaaatacga
    16141 aactggatcg gccctgagga tacaggcgca actaaaatag ttaacgctag ataaagagcc
    16201 atgatgatgc catttacaac gattacggat aaccgttgtc tgcttttcgt tttttgcatt
    16261 ttctctcctc ttttctccta gtttttttaa cgcgggatgg ttgatgaacc gcggttatgt
    16321 gaaacaaaca ataattataa cagacttttc atttatttca atccgtctta tctgctttta
    16381 aaaaagcaaa agcttaaaca atgtaaacgt ttgctaaaaa tcgtgggata tggtataaag
    16441 taagtgtaag gggtcataag aattcccagt aaagcagacg aagtaggatc tgcaagaaca
    16501 gaattgttgt gaaacttaaa taaataaaaa aggatgtggg aatttgagca acctcttact
    16561 agagaacggt ctagcaagca gctgaagatc taagtggaca tgccgaacct tggaacttct
    16621 ccttacgtaa gtaatggatc atgcgcttgc taggcttttt tgtgtttttc ggtcttcgga
    16681 cctttttcat tttcctagac ttctgctata ataaccaaga attaattttg taacagattt
    16741 ttttgaaaaa acaagtttct ttcattcaaa aaatagaaaa agcttgctaa ttattggtta
    16801 accagcaaac aggagtatta aaatatcttg ttttattgaa tagcaggagg aaatcatgaa
    16861 attccattat catatattga tcaatccatc ggcaggcagc ggaaatggct ataaagtagc
    16921 agaaagaatt ttaccggtat taaaaaataa gcatattgac tatacacttc attatagtga
    16981 atacaaagga cacgatgctg agattgctga aactttggca aaagaaacat tgattccttg
    17041 gatagaagaa gaacagaaaa caatcgatac tttccccctt ttgatcatcg taggaggtga
    17101 cggcacctta catcaggtac ttgatacttt ctatcagatg gaagtagaat tcccagttgc
    17161 ttatattcca gcaggttctg gagatgattt tgctcgaggg gcaggccttc caaaaaatcc
    17221 caaaaaagga cttcagctga ttttagcagc tcaatcacca gaaaaagtcc acataatggc
    17281 ttatgaagaa aaaataagtg aaaaaaaagg aatagctgtt aataatttcg gtataggatt
    17341 agatgcagct atcgtccatg cgacaaatca ttcttccaca aaaaagcgat tgaacaaata
    17401 caacttagga tcattttcgt atttattctc tattctccgt gctttattca ctcaaaaagg
    17461 atttccaatt ctggtagatg caggaggaaa acaattaagt ttttcaaacg catttttatg
    17521 tacagcaacg aagcatcctt tttttggcgg aggaatcgcg attgttccaa ctgctgatgc
    17581 gtctaaacca gtcattgatt ttgtcgtggt cgaacgaatc aatttcttta aaatcttgtg
    17641 gctcattctt ttacttatac agaaaaaaca attgaaatcg aaatatttcc atcactacac
    17701 aacaaacaga ttacgtatcg tttcaacaac accgcaacat ggtcaagaag atggagaaga
    17761 aatggaaaaa cgaccttttg atatcacaat aaccacaact aaacagttgt tttggtgtta
    17821 ataaaagagg ttttgtcaga agctacatct ggaacaccgt ttattcggaa aaaaggactg
    17881 tgacacgact tttgtcacag tccttttttt actatgcagt taattctttg cccaattggg
    17941 ttgccatcga tagtttcttt ctttacccaa aataaattgg atcattgaaa acaccgcagc
    18001 aaaagcaact aagacacaac cattgaacca aaaagattct gcaaatgttt gattgtaact
    18061 gagtgtttca ctgatcaagt tattattttg aacaaaccaa tatgttggtg tgaatgccgc
    18121 tattttgcta accatttctg gcaagtattc tgctggtaca aacaccccac cgataaagca
    18181 actgcctgtg ataaaaatat ctttgatccc accgctggtt tctgagtttt ttaccaatga
    18241 agtgatacaa gccgaaaagc taatgatcgg caataaaaac aagaaagtat ttaaaatatg
    18301 ataccatacc gttatatccc acgaggtgtg gatcactaac agaaccatta gaagtaagaa
    18361 gccactgaac aacagcagcg agtaactaat caaactaaag gttactttgc gattcaagtt
    18421 tcgtttggta atgggcgcac tctggtttcg tttactgatc tctttgcgat taaatgctag
    18481 attgatgacg ctgaaaccgc taaaaatgga tagaaacaac ccataagaca agaaattaaa
    18541 ggttgtgccc gtcaacagct ttttcatccg ctgactatat cccgcactta gctcaacttc
    18601 tccagtaacc gagagtgcca tttgtgtctg ttccatagct gcatcgattg ttttatcttc
    18661 ttcttgaaac agaatcagtg tttgcaaaaa ggtgttcact tgctgtgtaa caactgtttt
    18721 agaaaaggca tctgggcgac tctgtatctt gatcgtcggc atctttccta cttcgacttg
    18781 ttcggtaaag tctgcaggca gatgtaagat aatgtttaca cgattaaaat aaagagcatc
    18841 atcgatttct ttttgtgagg ttccatccaa tgtaacgact gtttccttgt cattcaaata
    18901 tttacgaagg ccatcgacta caggcgactc ttcttcacta atcaaggcga ttttcgcatc
    18961 ttgcagagag gtattttcaa catcaatatt ttgcgcaaaa aagaatgtgg caacaacagt
    19021 gataattaca cctatcagta aattcgattt atttgcttta agcaatgtcc aaatcgtttt
    19081 ataaagcgtc ataacgcacc cctctttcca tccaagcatt tcctaacgca aaaaggaaaa
    19141 taaatccgat caaccataga agattggtat aaaaactagc caaatcagtg tagaaataaa
    19201 gtttatacaa actttcgctg atcaagttga cgagatttaa ttgaccaaca atcggaatat
    19261 gaagatcaat ccaatacttc acttgttccg accccatcat accagcaaaa aagctcatcg
    19321 caatcgtcac tgaaatcaag atgctatcct tttgcaccag attcattttt ggtatcaaat
    19381 taccaaccaa tgtacctaac aaaatcgcac acaacgaccc cagagcggta acaatcatca
    19441 accattgcca acgttcacca aagtctactt gatagataaa acgaaagaca gctaacataa
    19501 tataaatctc tgtcaaaaca atcgtaaagc ttgctgccaa attagcgatt gaaactaacg
    19561 attgctttct aggaatcaag gacaagcgaa taccatttga cgattgattt gcttgctgat
    19621 cctgtacatt gcggaccccc cacatcgtcc cgtagagaat cgccattcca atcagagtga
    19681 agaaataaaa acttttgaaa ctcatattac gacctgcatg aacttcttga aaagatactt
    19741 cttgttcaaa aacagtcatg atgggcgcga gatcaacgtt ctccacttct gccactttac
    19801 tcgaaattat accaatacgc tgtaaaaatt gatccatgac gtttttcatc aacgtctgac
    19861 tcattcctgc ttgacccaca tggagcgaaa cagatgaacc atcaatgaga taaaaaccag
    19921 ccaaatcatc tgcatttagc ttttcattcg cttctacttc cgatagttcc tgtacttcaa
    19981 ataatggttt atcattgact tcgacttgtt ctaagactgt ggtaaaattt tccgaaagcg
    20041 ctgtttgatc cgctttgatt acaattccag tattaatcgt ttcgagattg ccaccttgat
    20101 caatctcgcc aaaggccgcc ataaacatca atcctaaaag aattggaaag gccaatgtcc
    20161 aaaacaacat ggatttattt cgcagcagta tttttgtgcg atatttataa aaatgccaaa
    20221 acattatttc ccctcctcta gtctctcaaa tccttgccag taatctctaa aaagacatca
    20281 ttcaaggacg cttctttggt tgctattttt ccgtaactga tcttttcttc ttgtaagatt
    20341 gtaagaatcg gcaacaatac tgtatttagt tcagtagttg ttacttggag ctgacctttt
    20401 ttataagcga ttccatcaga agctattgtt cgctgacaac acgttttctg ggagtaatgg
    20461 gatgtcgatc gtcaaacgat ccgccgtccg taccatatct tttagagatt ctttggttcc
    20521 tttagcaatc acctgtcctt gatccatgat cacgatctgg tcgcaaagaa tttctacttc
    20581 ttccatgtaa tgtgttgtat agacaatggt tgctccttgt cgattcagct ccttgatact
    20641 ttctaaaatt ttattccgac tttgggggtc tacagctact gtcggctcat ctaaaaaaat
    20701 taaccgcggc ttgtgggcaa tcccacatgc gatgttcaac cgacgtttca agccaccact
    20761 aagttctcct ggacgaaacg aaacatattt ttctaatccc actaattgaa tcacctcttc
    20821 aatcaaggct ttccttttct ctttttcgga aacatataaa ccgcaaaaat aatcgatatt
    20881 ttcttgcaca cttagttcgt caaagaccgc aacgtcttga ggaacgaccc caatcattgc
    20941 cttgacagca taattttcag gagtcattgt tgtcccgaac aatttaatgg ttcctttgtc
    21001 ataatttaac aaagaaagca tacagttgat ggcagtcgat ttcccactgc cgtttggtcc
    21061 taacagacct aagacttctc cttcttgtac ttgcagatca aaatgattca atgctacaaa
    21121 atctccatag cgtttgacta atttatcaat ttcgataatc atcatgaaaa actccttcca
    21181 aatttcttgt tgatctgact ataataaagt gcaagaaaag aaactagtga cgattggcta
    21241 aaccacacat gacatttgtc acaatgaagg agtctgctat gcataaatct cggctactca
    21301 acgaaactag ctttctactg gttatctcat tgcttttgct ggtaagtcaa ggattcgttg
    21361 ctatgcaagt tatctattta ttgatcgccg ttttattcac tggtgtcagt tatttactgc
    21421 ctacaaagcc aaattggatt ttccaagtta ttctatttct tttatgtgcc tttttacctg
    21481 atcttgtcct gtttctgcca gcgacgatgc gagtgatcgc ccctgaagaa agccaccttg
    21541 attacttgtt tttgttaggt tttttgttga tttcgacggt tggtaatcat ccccccatca
    21601 aggtcgcctt tgtcgtaatt ttgctgtttt tttcctttta cttacgaaaa aaagatcaac
    21661 gaacgaccga tcttgagaaa aagtacattg tctccaaaga tactcattgg gaaaaacaac
    21721 aagagttatc ggaacaaaat aaagcattgg ccctgtcaca agaaacacaa ttagccttgc
    21781 aaattgtgga agaacggaac cgaattgccc gagacatcca tgacaatgtc ggtcatctgc
    21841 tgtcaagtgc gattttacaa attggtgcgc ttgaaatgat caatcaagaa ccaaaactaa
    21901 aagaaccatt agaaaattta agtgccaccg ttcacactgg gatggatcgg attcgtcaaa
    21961 gtgtccatga cctccatgag acatcgatct cttttcaaca ctccttagag tttctgatcg
    22021 ctgattttcc ttcttctgtt acgattgaag gaaacttgtt tgaaggattg aatgaaacgc
    22081 agcaaaactg cttcttaatg gtcatcaaag aagccttaac caacagtatc aaacatagtc
    22141 atgccaaaac cgttgtttta cattttcgaa cgttgccggc cttttatcgc ttgcaaatca
    22201 cgaatgatgg caccagccca caaaaaaatt ctgctcccgg tattggtttg aaaacgatgc
    22261 gtcaacgtgt tcaagaacaa aagggacaag tccatatttt ccaagaaaac cagcaatttc
    22321 agttaacgat cattttacca agggagggac accaatgata acagtagtca ttattgagat
    22381 gatccatttg tgacgaaatc gttacaaacc attttagaaa gcacgaaaga gatccgtgtg
    22441 ttaggcattg gtcattgtgc caaggatgcg ctagtcttat acgaaaccca tcgtcccgat
    22501 gttttgctga cggacatccg catgcctaaa caaacgggca tcgatgctgc caaagaaatt
    22561 ttggctaaat ttcccaaagc catccttttg cttttgacga cattcaaaga tgaagaatac
    22621 atccgcgaag cattttccat cggtgtcaaa gggtatttaa tcaaacagaa cttgcaagcg
    22681 attattcctt ctgtcaaatc cgcttataat ggacaggccg tatttggtaa tgaaatcatc
    22741 gagactttca ctcaaatgat gaagaataaa ccgacgattg atagtcatgc ttatgcttct
    22801 tttagtgaac gtgagcttgc aatcatcaaa gaagtagctg ccgggaaaaa caataaagaa
    22861 attgcagatg ccttgtactt gagtgacggc actgttcgca attacatcag ccaattacta
    22921 gaaaagctag atttacggga tcgcacgcaa ttggctatct atttttatca gcatttgcaa
    22981 taagtcttat ctaacacaaa aaaacctgaa tgatgctcct tcgcatcatt caggtttttt
    23041 tcacatcttc tattcaattg tcccgcaaac catttcctca cgattgaaag aatcaaggat
    23101 tcgcagggct gccaggattc gttggattgt tcggcgtgtt aggcggaaaa ataggcgcct
    23161 tgtctaacgt tttttctagt tttgattctt cacgatcgtc tcgttctaca tcaggacttt
    23221 cattcaattt gaattctttt tccatctcat ctaacttttt aaagtcattg ccagattctt
    23281 ttgctaattt cgctaattta tcttcggttt ctttagccat aaagtaacgc cccccttttg
    23341 atttcagctt agcatatcac actgaaaaaa caaaagaaaa cgctaaccaa cgatcatttt
    23401 tagctatttc tcatctttaa aatcaagcat tttttgccgg tataaaatcg tagggagaac
    23461 tgttttccat accaatcatc ggatctacta actggttatc gatcacttct gctgtaagaa
    23521 cgaaatcttt agaaacagca ggttctacct cttctttctt ttcttctcca acaaatgaag
    23581 aaaacagatc tggttccact gtttccatta cttggttttt cgtttcattc gtcccactgt
    23641 catcttctac aggtagcttt tcttttacaa cccgtggctc ttttgtatag ttcccatctt
    23701 caaatggatc atttccgacc aaagaatctt cataagctgc tagtttggtc cggagagtga
    23761 tattcatttt atcattatcc atgatccttg atttcaacgt agtcatccgg cttgctgatt
    23821 cattcgctac acaagtgaca taggcttgct cttcccctaa caaaatcaac agttccttac
    23881 tgcgagtcac tgccgtgtag agcaaattac gctgaagcat ccgctgatat tgatggacca
    23941 tgggcaaaat aaccatcttg aattcactgc cttgtgattt atggattgag caacaataag
    24001 aaagagtgat tttattccat tcgtttcttt tataagtgac ctcatttgca tcaaactgga
    24061 taactaattc atccacttta tcttcagaat cttttgccaa gatgataccc gtgatttctc
    24121 ccatatctcc attgaataca ttcaattcgg gagtattgac taattggagg actttgtctc
    24181 ctatccgata aacggtgtcg ttccaatgta cttccttttt tccaggtttc cctggattga
    24241 atatctcctg catcatttta tttaacgcat cgatccctgc aggtccccga tacattggtg
    24301 ctaatacttg gatatcttgt gcacggaatc ccttattttt cgctttttcc acaattttag
    24361 agatcaaggg ttcaatttga taagcatcac ttcggaaata ggaacgatct ttttgattct
    24421 ttgtgaaatc ttttggcaga cgtccttctt tgatctcatg ggcaagagga ataatactag
    24481 aaccatcacc ttgccgataa atctgattga gttccatttt gggaatttgt tcgatttcca
    24541 aaagatcatg gagtacttga ccaggaccga cagagggaag ctgatctttg tctcctacta
    24601 agataacttg catattcgtc ggaatcgctt tgaataatgt gttagccagc catgtatcga
    24661 ccatagacat ctcatcaaca atcaaaagtc cgccttctaa ttctttcgta ctgactgaag
    24721 gtgttttttc ccgtccattc agccccagaa gacgatggat cgtactggca ggcagacctg
    24781 tcgtttcgtt cattcgttta gctgctcggc cagttggtgc tgcaagtaga ataggaaaaa
    24841 tttcttgggt atattttgaa ggatccaatt ccagtccgtt caactctgca aaaagattga
    24901 cgattccgtt gatcacagta gttttccccg tacctggtcc tcctgttaaa ataaatagag
    24961 gggaacggat tgcttcagcg atagcttgtt cttgcgaagg tccatactgt atgccgaata
    25021 ttttttcgat acgccgaata ttcttctgca cttctttttc aggataatgg atttcttttt
    25081 tacgactgag aaggcgctgg atcgaattac cgatccccca ttcagaaaag tacaaactgt
    25141 tttcgtaaag attagtctct tcttgttgta tctttccttc ttcaacaagt tggataatta
    25201 cagtagcaac ctgatcaggc tggatttcaa ttgggcgact tgtttctaaa atacggatcg
    25261 tttgctgaag caatgtctcc gccggtacgt atgtatctcc tgtttgaact gcatgctgaa
    25321 aaatctgatg caagatagct gctcgaattc ttctcggtga atctgctgca ataccgattt
    25381 gttcggctag attatctgca cgtttgaacc cgattccttc tatgtcttcg actagctggt
    25441 acggattttc ttcgatgata tccaaagact cgttcttata ggcctggtag atactgaaag
    25501 ctaactgact accgaacccg taacgattca gtcccatgat cacttgatcc atcccgtggt
    25561 ttagccgaat cgtctcagca agcatttctc tttttggctt cgtcaaaccg gagatttgtt
    25621 ttaataccga aggatcagca atgatttttt cgatagcatc ttctcccagc aattctacaa
    25681 ttttttctgc tgttttcttc ccgattcctg ggaatttttc gctggaaaga tacgctacga
    25741 tcccatttgc agttgttggc ttttcttgct catatctttc gagtttaagc tgccttccgt
    25801 atttaggatg atccactaat tctccataaa aacgataagt ctcattttct tgaatatcgc
    25861 cgaaacttcc ggtgactaca atctcttttt cacggtaatc agctgtggtt tcactgatgt
    25921 gtaccaaaag cactttgtaa aaattactag gattttgaaa aaaaatcgcg gcaactgtac
    25981 caacgaaata aggcatttct tctggctgca tcggaagccg cctccttctt ctatagtaaa
    26041 ggttctggtg ctgtttcagt tcctaaaaaa tctgtttggt tccatacttt cagttcatat
    26101 ggcaataatc tgcaaggctc ttcagtctct aaaatcgtca aactactatt gaacaatcca
    26161 cccatttcac gcaactcact tagctctttt cctgtcatcc attgaatggc tgcagttaaa
    26221 gaagctccat gtcctacgaa taaaagtggg ccatctttat gttgggcaac cgcatctgct
    26281 acaacagttt cgatacgtgt gattgccgat tcaatcgttt ctccgtgaaa aggagaagga
    26341 tcatacagat ctaaatgatt acgcaaattc ggcagattat ccggatattt ctggtacatt
    26401 tcatcgatcg actgaccttc taaagtaccc agccctagtt ctcttagctt atctgtataa
    26461 aaaatctcta ccggacgagc aagctctttg ttgatacctt ctgctgtatc acgtgctcgc
    26521 tttgctgtac tggaataaat tgctttaaaa ggaacatatt ttaccttttt cccaaatgct
    26581 ttgatttctt cgtaactttg tggcaaaagc ggagaatcgc cattgctccc ttgaaaacgt
    26641 ctttcctgat tccattgtgt cttcccatgt cgggtaaaat atagttccat acgcagcaaa
    26701 ctcctgtctt tcttttattt tactctataa taaactgtct aatccagctt gtcattttca
    26761 tagtgatgcg tcaattctaa attagggaat ccttgctggc gaagtgcttc atacactatc
    26821 attgcagctg tattcgataa attcaatgag cggacatgct catcattcat agggatgcgt
    26881 aaacactttt cttcgttttc acgcataaat gtttctggca aaccggttgt ttcttttcca
    26941 aataaaaaga agtgctcttc tccatcagca aaattttcgt cactatatac tttatgggca
    27001 aattttgaga tcaaatgtag tttacgatta cctaaaaatg ttaaaaaagc ttctaaatta
    27061 ctgtggtaag taatatttac atcattccaa taatccaacc ctgcccgttt tagatgtttg
    27121 tcatcagttg aaaaacctag tggctcgatt aaatgaagcg gagtattcgt ggctgcacac
    27181 gtacgggcaa tatttccagt gtttgcaggg attaatggtt caaataaaac gatatgattc
    27241 atcaggatca ttcctctcat atttatgttc gtttctattt tctgacaatt tgatttttgc
    27301 ataaaaaaaa acgtatcaac tcggtgtcag tcactgcgag tcgttacgtt agtgaagctc
    27361 ttctcactaa cttttatcag aacaggaacc tgataaaaac caatgaaaaa acaacttgtt
    27421 gctaatataa caccctcaac ttttttttgc aacctctact cattaaaaac tgctagaaaa
    27481 acgttttctc ttagttcctt ttaataattc tatgtcaatt gtaatcttct tcaaaggatt
    27541 cttatataag tctgctttga ttcgctcttc tacctgccat tctaaaggtg acatggccaa
    27601 aagatctttt tgattttcct ctggtatctc aaattcatag gttacacgct gacgttcagt
    27661 taatttcatt gcttcagcaa acttggctac tacacgttca ttggaataac tctgtttttc
    27721 aggttgattc ggataaaaag cctgacgtag ttcctttaaa taattttctt gtggcaccac
    27781 cttcaacact tctccatctt ctgagagtac acgttggaat tcttggtaat gagaaggcga
    27841 aaagatattt aaaaccgcgt tgaatgtttg atcagcaaac ggcaaattgg tcaaatcagc
    27901 tacacaccaa aatgcttcgg cagattgttc tgtagctgcg tagactcctt cttttgaaat
    27961 atcaaatcca acagcatttt gaatcttttt caattttgaa agttcattca agaagctacc
    28021 ttctccgcag ccaacatcta atagcgagac ttcttcaggt aaatgctgcg caatagtctc
    28081 aataacgggc tgatacatgc cagattggat cattctacgc cttggaacga acattccttg
    28141 atcgtattct gttttaaccg catgggataa aaaataaagt gttccttttt tggaaaggtc
    28201 aaaacggtga ttatttttgc atatcaagcc attatcagac ttttttactt cttcttggca
    28261 aaccggacag cggaacagct gatgattttc tgccaaaaac tggcgtcctc ggtcaatttt
    28321 ttttaacata gttccccctc atttctgttt tatcttaccg ttagatgctc actttggcaa
    28381 ctgctaaaaa tatgatacaa tgcctagaag aaagtgaaag ataaaggaga agattagatg
    28441 atcaaagaat tttgcgctga aaactacaca tctattcctc tagcaattgc tagaggagcc
    28501 gatcggatcg aattgtgtga caacttagcg gtcggtggca caaccccgag tactggtgtg
    28561 atcgaagaaa cgttaagtta tgctggggaa aaagatatcc ccgtaatgac gattattcgt
    28621 cctagagggg gcgacttcgt ctataacgat atcgaattga aaatcatgca tactgatttg
    28681 attgaagcaa agaaattagg gacggatggc gtagtatttg gttgtcttac tccttcaggc
    28741 tggctagacg aagaggcctt agaactattg atcgaaacag cagaagggtt gcagatcacc
    28801 ttccatatgg cttttgacag tattcctgaa gatcggcaat tcgaagcaat cgattggctg
    28861 gtagaacatg gtgttgaccg tatcttgact catggcggtc ctgctgacaa aactattgta
    28921 gaaaatttcc cacggcttaa agaattgatt gcctatacag atggccgctt aatcattttg
    28981 cctggtggtg gtgtcactgc agagaatgca gcacatgtag ctaaagaatt gaacgtaagc
    29041 gaagtacacg gaaccaaaat cgtggatctt cagttataat cttataaaca aaaaaacatt
    29101 tgcaacaggc ataatgctta ttgcaaatgt ttttgttttt ttacataaaa tagcccataa
    29161 ataaagaagc aatattgaaa taaatcagta cactcgtcaa atcgctcaaa gtcgtgataa
    29221 agggaccgct tgctactgct ggatcaaatc ctagacgatc cattagcatc gggattaaac
    29281 tacctgcaag attcgccact gtgattgcaa atagcatagc catccctatg acaaacccca
    29341 agataaaatt atgtttccaa aaacctacga ttagaaaaat cgtgatcccc gtaacagctc
    29401 ctgtgacaac gccggtcaag acttcagcca aaacaagtcg aacaaatcca tgatctttct
    29461 catcagaaat cgcaagtcga cgaacagcca cagctagcga ctgggttcca gcattaccgg
    29521 cagttcctgt aatcagcgaa atgaagactg ctaaaatact cgcttcactt accaagtctt
    29581 catagtgact gattaaagaa gcggtcgaca tacctaaaaa caacagagtg atcagccaag
    29641 gcaaacgtcg agacgctgcc tttactggat tttcattaat ttcttccaca ttgaccccgg
    29701 caagtcctga ataatcgcta gcagcttcat catcaataac gtcgatgata tcatcgactg
    29761 tgacgattcc taacaaatga tcatcataat cggtaacagg aagagctaag aagtcataat
    29821 ctctaaatgt ttgtgccaca tcttcttggt cgtcgcctac atgaactgaa agcactcgtt
    29881 cgctcatcag atcagaaatc attgcgtcgt catcattcac gattaggtcg cgcagagaaa
    29941 taacccctac tagctgacct tcttgattaa cgacatagat gtaatagatt gtttcagcta
    30001 catctgcttc gttcttcagc acgtacatcg cagagcgaac agtttgattg gctacgatcg
    30061 aaacaaattc agttgtcatg atcgcgccag ccgtttcatc ctcgtaatgg agaagttcct
    30121 tgatttcact ggcatcgtct gccgacatca aacttaaata tttagcaatt tgctttttat
    30181 ccagcgtatt aagcaagtca acagcattat ctgtatacat ctcagaaagc atatcagctg
    30241 catagctagg acgcatttca tctaggtatt ctttcatatg ttcgttgtct tcttcaatca
    30301 catcgaacat atccgctaat tctttaggcg acagatagga atagatcagc tgcctgttct
    30361 cttctgtaag agattggtag aattgcccct gctcatagat atgcatctct aaaaaactgt
    30421 ttctgaatgc ctgaatatta ttttccctta aatcttgagt caactgggca aatctttcct
    30481 ctaattcttg attttcatcc aaaccaatct cacctcgcct tattcttcaa accgtctcac
    30541 aactgcctcc atgtctgcgg ctaaaggctg cttgaactcc agcaactctt ctgtaaatgg
    30601 atggtaaaaa cgcagatcat aacaatgaag ggcttgtctt tttattccca gatccattcg
    30661 cccgccatac atatcatcac cgagcagggc acagccaatt gcggaaaaat gaactcttat
    30721 ttgatgtgtc cttcctgtat gcagctggat atcaactaac gcgatttctg ttagtcgctt
    30781 ctctatccag tactcagtct gtgccgaacg tccttcatca gagaccattc gcttcaatag
    30841 cgaagaataa tctcgggcga tcggcaaatc gatatgtcca tgtgccgata aatctgaaag
    30901 ccttccactg actaaggcct gatatttttt cacaaacttc ttttcacgaa gttgcatatc
    30961 taattttgcg tgcgcaaatc catgctttgc aaaaagcatc aaaccagatg tatcccgatc
    31021 tagtcgcgtc accacatgga ttacttggtt ttcgtaacct tgttttttat aataagcctt
    31081 tacacgatta gccattgtcc catttggatg gtattgggca ggaatagacg aaacgcctgc
    31141 tggtttattt actaccagaa cgtgttcatc ttcatacacg atatcgatcg gtgtttcatc
    31201 caaaagtacg gtttcatgtt ctccttcagc aggaatcaca atggttacct tatcatcctt
    31261 agctagagaa aacaagacat tttctacttg atcattaact ttgatttgac cgccttgaaa
    31321 ttttattttc gctaacagac cctttgagat ccctttttct tttaaaaaat attttacttg
    31381 ctgagaatga tctttctcat aatcccatgt aaactgcata tcaatcctca ctaataaagg
    31441 catctttcac acgatgccaa aaatgcatat gtcgataaga agcaaaatgg atccgttctt
    31501 ctgctattcg ataataaatt gagcggatat ttgcttttgc gacattcaat tgatcaaccg
    31561 ttacaagata atcttctgta ttttccaatt ttatttctag ccattcatca tgggcaatca
    31621 cgatcggaga acccaaagta cgaaacactc gattattcag tgaagcaatc tctgccaact
    31681 gaaaggcatt gatactagga tggatcacag caccgccgac acttttatta tatgctgtcg
    31741 aaccagtagg cgtagaaatc gtcaatccat ctcctctaaa actttcaaac agctcgtttt
    31801 tgatgtatac atcggctacc atcgtacgat tggcccgttt aatcgttgat tcattcagtg
    31861 ctaaaaaatg ctggtccggt gtttcatcca aataactgat ccgaacatcc agcaagggat
    31921 aactaacact ttgttcccgg ttcgttcgta agctgtctac tagttcttcc agctcgtaat
    31981 cccgccaatc cgtgtaaaac cctaaatgac ctgtatgcac gcccaagaaa cgcacatcat
    32041 tcagacaatg actgtagtga tggaacgccg acaataatgt gccgtctccg ccgactgaga
    32101 tgaccaattc tggctcatga tcatctcgtt gaatccctgc ttgttctaaa agtgcgttca
    32161 aacgttctgt cacttctata gtctttgctt ctttattatg aacgatcgct actttcatcc
    32221 cctgcctcct ctctttcgct ttctttcttc aaaagcgttc gatagtagac atcatctgat
    32281 tttcctcttc catgggagaa taaccgctgt gcttcttgga tttcttcacg gatactggac
    32341 atctcttcat ccaactgata ggcagcttct gccgcacgct gaagtcgttc actcatttct
    32401 tctggaaaaa cgccttgata tttataattc agcgaatgct caatcgttgc ccagaaattc
    32461 atcgcaagag tccgaatctg gatttccgcc aaaattttct tctcaccgct gatcagctgt
    32521 acaggatact cgatcaccag atgataagaa cggtagccac ttgctttttt atttgtgatg
    32581 taatctcgtt cttggagaac tttcatgtct tttcttttac ggatgatctc aacaacttga
    32641 tggatatctt ctacaaactg acacatgatc cttaacccgg caatatcaga catttcttct
    32701 tctaaacgtc ccattggaat atgccgtaat tgtgattttg ttacaatact atcgactggc
    32761 ttgactctcc cagtgacaaa ttcaatcggg acatgtcgat tttgttcacg gaactgtttg
    32821 cggatccccc gcagtttcac tttcaattct gatacagttt gttcatatgg tgctaaaaat
    32881 gcttcccaat ctcgttccat agctgatacc tctacttcat taacattcat tgtttattct
    32941 accatatatg caaagttttt tcgtactctt gatttatgca ttgaccaaaa aaagaaaatt
    33001 aggcaaaata gacaaaaagg agtcgatcaa atgacacaaa tgcttgaaat cgaatataaa
    33061 tctatgctga caaagcaaga gtacgagata ttcatcgctc actatcaatt aacagataaa
    33121 gatttccgtg tacagactaa tatttatttc gataccgttg atgaacagct gaagaaacaa
    33181 aattgtggcc tacggatccg tttcgttggt catcaagctg aatatacatt aaaaacacct
    33241 gctgaaaaag ggcgtctcga aacaacggat acctttataa cagaagaagc agaagcattt
    33301 atcttcgaaa aacgtctgcc tcgttcaggc gcagtttttc aaaaactttc tgatttgaat
    33361 atcgatcctg cttcattaat ccaaaccgga aagcttacaa caaaacgggc tgaattccct
    33421 attgaagaag gacttttagc tattgatgaa agctggggag acaatctaca cgattttgaa
    33481 ctggaactag aggtagggga tgcatctgtg ggaaaaattg cttttataaa ttttttaaaa
    33541 cgtttttctt taccttatcg tcctgctaag aacaagatcc aacgtatgct tgaagctaaa
    33601 aattagaaac ctttcatttt cacaaggcac ttgagttcat ttttttactt ttttctgtta
    33661 agttatagga tgtgaaagaa tctagtcact agttgtatag aatctatttt gggggagatt
    33721 catatgatcg aaatctattt atttgttaat cctttagggg aatactgttt cgaaatggaa
    33781 caacagttat tacaatttat cgaagaagag tacgggacac catcaaaaga aaagatgcaa
    33841 tttcgctttc ttcctttggt caatttgcag acaattgggg atgtcatgca acaaaaagga
    33901 atttctcaga acaatcttga ggaacgtaat ctcttattct caatgactta ttctgcagca
    33961 ttggactgca aagcggcgca gtttcaagga aagaaaaaag gccgccgttt tttgatcaaa
    34021 ttgcaagaag cagtcggctg taatgacata ccatattctc aagaacttgc tgaatccata
    34081 tttgaagaaa tcggcggcga tttgaatatg tttaaagaag atcgtcagtc tgacttcgtt
    34141 aaagaagtat tcttttctga tcaaaaaatt gctcgggaaa tgggcatcac aaaacaccct
    34201 tctgcagtcg tttacaatta tgcctgcgac cgtgattttg gtgttttagt agaagtaaaa
    34261 ggtacaagta gcctttggag aatcacagag ctgtgtaaaa caacagataa ctcatatcaa
    34321 gtttttaatg gtgaacattt acatacaaat gattctcagt tccattcaag aactagccat
    34381 ttacacttgc tttctaatta aaaaaatacg tcgactccct tacaagagag ccgacgtatt
    34441 tttttcgttt ctattcgaga aaatcaaaaa tagaaagcct tataaacgat cgatcaattc
    34501 ttctaattca tttagacgtt gttcaaacat cgacattgca tcttcaatat atgcagcctg
    34561 tgtcatatca actcctgctt ttttcattac ttccacagga taatcgctgt tacctgcttt
    34621 taggtaagcc aagtagttct ctagtgcctc tggttcctgg tttagtattt tcttcgccag
    34681 tgctgaagca gcagaaaagc cagtcgagta ttggaaaaca tagtaattat aataaaaatg
    34741 cggtatccgt gaccattcga atttaatttc tgggtcttct tcgactgctg gaccataata
    34801 ttttgcattc aatttaccat aactatcact taggtattca ctagtcaatg gcacaccttt
    34861 ttcatcttcc gtatgcatga aatgctcgaa ttcagcgaat tgtgtctggc ggaaaactgt
    34921 tcctttaaac ccatccaagt aatggttgag tacataagcc cgtacacgag ggtctttttc
    34981 tgtttccaat aaatactccg ttaggatatt ttcattcgtt gtcgaagcaa tttctgctaa
    35041 aaagatggag tagtcgccgt acacataagg ctggttcgaa cgagtgaaat aactatgaac
    35101 actatgtccc atttcgtgga caagcgtaaa tagctgatcc agcgtatcat gccaattcaa
    35161 taaaatatat ggatttgtgt catagcttcc cgaagaataa gcatcgcttc gtttcccttt
    35221 attttcgaca acatcgatcc aacgttcaga gaatgctttt tctacgatgg ccatgtattc
    35281 ttcacccatt ggtttcagtg cttctaaagc tttttctttt gcttcttcgt acgtaaaggt
    35341 aattggagct tcccctaaga ccggtgtata aagatcatac atgtgcagtt tttccacttc
    35401 taataaacgt ttccgtaatt ccatgtatcg atgtaacaaa ggcaaatgct tgtttaccac
    35461 gtctaccaaa gtatcgtata cactttcagg aatatgatta ttgctcaaag acgcttctct
    35521 ggcagagctg taattacgga ctttcgcttt aaaattatgt ccttttatat gtgtgcctaa
    35581 agtagaagca aatgtatttc taaattgttc gtaaacactg tacaatccct taaatgctgc
    35641 ttcgcgcacc cttcgatccg tgctttctag caactgacca tacacgccat gagataattg
    35701 gactatttca ccattttctc cttcaatcgt tggaaaaact agatctgcat tattcaaaac
    35761 agcaaatgta tcacttgaag catcaaagat ttcacctgct ccagcaagaa gagattcctg
    35821 ctcagcagat aagacatggg ctcgattatc tactatttgc tggatataat ggcgatagac
    35881 ttccaatttt ggttcttctt tgaaatattg ccaaatctgg tcatctgaca attgcaatat
    35941 ttctggttca aaccaggaaa cggcttcact aactttcgaa aacagactgc ttgctcttgc
    36001 ataaagcgct tggtaatctg tatttccagt gtcttgatcg tttttaaggt gcgcatatac
    36061 ataaatgact tcagtttggc gataaaccct caatacgaat tcaatcgcat ttaaaaattg
    36121 agaagcgcct tgatcaagtg ttcctttgtg tttttcagat tgttttaact cttctgataa
    36181 ttccaaatat ttctcatcaa actcttggtc gcttgaaaag atcttggtca agtcccaagt
    36241 taaattttca ggcaattctt ctcgttttgg caactgcttt acttccatga aatccactcc
    36301 ttttcttccc ttagctctat ctatcaaaat agatcatagt ctaacatcca catgtcacag
    36361 catctaactt ttctactcat tcatgtgtct gcttccatca attttagcat aactaaatct
    36421 tgttttacat agaaaaccga ttcctagcct atttttatac ttactatttt ttcaaagaag
    36481 acaagtgtat gcattcgtta aaaaactgct gatagaccat ctcttcatca atcataggaa
    36541 aaagccattc ccttttatgt tcttttattt ggcaaagcca tgagttgaaa tcaggaactt
    36601 tctggttttc caaggcttct tcgtacaaca ttcgataaag aaaaacctgt tcttgtaaat
    36661 aaaagaaaaa ccgacttttt tgatagatcc acgatgatag atgcaaaaga tgtttatgac
    36721 gcaagtagca ttgttcttgt atggatactg tattcttttg cttgtgaata agttggcgtg
    36781 tcagccattt cttcccatct tcactcactt tttctatttt tttcatttgg ttcattttaa
    36841 tttcgttact agtgaatatt tctttaaggt ccctactaga aaaagtccag cagctactgc
    36901 aaaaggaaat atgccctgat acagtctctg taatatgatg atacaaataa agcttttgtt
    36961 tcttgagatc agctttccag caatgcactc ctctttttct attatagtag cagcaacttt
    37021 tttctatcaa tgagaattgc gattgtccaa gaaaagaatt accaagtatc caccatactt
    37081 ggtaactgtt attcaaataa ttttgggttc tttcaataaa tctctggtga gacagtctag
    37141 aacattggat ttctattgct agattcgcca acaaaatatc cggcctttgc cgcaacctcg
    37201 gcaaataggc ttccaaaaat actggttcta ctgactgtcc cagccaatcc atgaagcatt
    37261 cctttaactg taagtgctct tttgattctc tttcactaaa gaccgaacag ttatctgatt
    37321 tttgatgggc aaaatgagca cattttttct ttcccttttt caaaatcact ctttttcgac
    37381 agccacgaca ataatatttc tttccttttt ctgcatgttt agcaaaacag cacttattat
    37441 attcattcat tgcaagcagc atttttctcc ctctgaactt atttacgtct aagaatttat
    37501 ttacacgaaa aaagagggcg tgaaaaagct attctgcatc aagtaataag aacagctaaa
    37561 caaaaatagt tcccaatatt tttcgtttgg ttgggcttgt taccgcagat gcaagctttt
    37621 gaacacattt atacgaaaaa aagactgcga cacaattttt gtcacagtct cttactataa
    37681 tgctgattta tacaaaataa aatttcgttc gctcgatagc attacgcgcc atcagacatt
    37741 gtccatattc attcaatgtt tctggtgtac gatcgctctt ttcagcaaac tccaaaattc
    37801 gagcaatctg attctctaca tccgcttggt tgagattttc catccagaag tgaacgacta
    37861 ggtaatagcg ctcttcgaat aaatacaaat cagtccaagc agactctagt tcgacatctg
    37921 ttgcaagttg gatcacttgt tcaaaatctt caaaagcaaa agttgcttgt ctttcctgtt
    37981 cttctagggc gcttccagat aattcttcct ccggatcgct gccaataatc tgctgacgaa
    38041 gaaagtcact cacatcttct gaatcaacag tagaatcttc tgattgagaa atttcttcat
    38101 ccggaagatt tttactgata aacagttcta atccgtctcc ttttggcaaa acttggaaag
    38161 tgacagcttc gcttcctcgg aactcctctt cgatatcaac ttcttccaaa atactataaa
    38221 agaaattttc gatttctttg tgattgccaa gtaaatccaa aaatgaaacg ccccgttcct
    38281 ctaaatcttc atgaccaatt agtacacgga tcgtattttc attaatatgt tccatttcca
    38341 ttttgctaca cctcactttg ccacaatgtt taatatagat tcattgtagc gaaatcagct
    38401 taaatgtaaa gaaaaaaaat ctcgctgtta agcgaattta ataaaaccag cggatttttc
    38461 aacctttcaa gctatcttat ttaactataa catttttggc agacgaatac tatatagccg
    38521 acttttcagc taattttccc tctctatttt tcgtgcaatc atcatcttct actacctttt
    38581 tatacatcta atcaagaata ctttttgtaa aaacaaaaaa acttggaaaa actatctt
//
LOCUS       DAZKKW010000018        36792 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.018, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000018 DAZKKW010000000
VERSION     DAZKKW010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36792
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.018"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..386
                     /locus_tag="ACG8PO_000916"
     CDS             <1..386
                     /locus_tag="ACG8PO_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFQ0206261.1"
                     /translation="GAVFELQDANGTVLQSGLTTGADGKLAIDGLAPGAYQLVETQAP
                     TGYELDATPVTFKIEKEQEVAIFLTKENRKVADSSDIRGNNPTSSGNKHLPKTGETII
                     SQFILSILGVLLVFISIKFRKKRNI"
     gene            complement(739..1587)
                     /locus_tag="ACG8PO_000917"
                     /pseudo
     CDS             complement(739..1587)
                     /locus_tag="ACG8PO_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1740..2852
                     /locus_tag="ACG8PO_000918"
     CDS             1740..2852
                     /locus_tag="ACG8PO_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFQ0206262.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            complement(2930..3223)
                     /locus_tag="ACG8PO_000919"
     CDS             complement(2930..3223)
                     /locus_tag="ACG8PO_000919"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HFQ0206263.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            3391..3705
                     /locus_tag="ACG8PO_000920"
     CDS             3391..3705
                     /locus_tag="ACG8PO_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HFQ0206264.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            3726..5705
                     /locus_tag="ACG8PO_000921"
     CDS             3726..5705
                     /locus_tag="ACG8PO_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFQ0206265.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            5718..6044
                     /locus_tag="ACG8PO_000922"
     CDS             5718..6044
                     /locus_tag="ACG8PO_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HFQ0206266.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            6060..6578
                     /locus_tag="ACG8PO_000923"
     CDS             6060..6578
                     /locus_tag="ACG8PO_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0206267.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            6578..7930
                     /gene="celB"
                     /locus_tag="ACG8PO_000924"
     CDS             6578..7930
                     /gene="celB"
                     /locus_tag="ACG8PO_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFQ0206268.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(8014..9519)
                     /locus_tag="ACG8PO_000925"
     CDS             complement(8014..9519)
                     /locus_tag="ACG8PO_000925"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HFQ0206269.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLEFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(9611..10201)
                     /locus_tag="ACG8PO_000926"
     CDS             complement(9611..10201)
                     /locus_tag="ACG8PO_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFQ0206270.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            10380..12026
                     /locus_tag="ACG8PO_000927"
     CDS             10380..12026
                     /locus_tag="ACG8PO_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFQ0206271.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYFQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            12075..12794
                     /locus_tag="ACG8PO_000928"
     CDS             12075..12794
                     /locus_tag="ACG8PO_000928"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFQ0206272.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            13273..14220
                     /locus_tag="ACG8PO_000929"
     CDS             13273..14220
                     /locus_tag="ACG8PO_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0206273.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            14232..15152
                     /locus_tag="ACG8PO_000930"
     CDS             14232..15152
                     /locus_tag="ACG8PO_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0206274.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            15193..16650
                     /locus_tag="ACG8PO_000931"
     CDS             15193..16650
                     /locus_tag="ACG8PO_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0206275.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            16995..17609
                     /locus_tag="ACG8PO_000932"
     CDS             16995..17609
                     /locus_tag="ACG8PO_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFQ0206276.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            17602..19326
                     /locus_tag="ACG8PO_000933"
     CDS             17602..19326
                     /locus_tag="ACG8PO_000933"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFQ0206277.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            19323..20774
                     /locus_tag="ACG8PO_000934"
     CDS             19323..20774
                     /locus_tag="ACG8PO_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFQ0206278.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
     gene            complement(21247..23400)
                     /locus_tag="ACG8PO_000935"
     CDS             complement(21247..23400)
                     /locus_tag="ACG8PO_000935"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HFQ0206279.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDREYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
     gene            complement(23402..24448)
                     /locus_tag="ACG8PO_000936"
     CDS             complement(23402..24448)
                     /locus_tag="ACG8PO_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0206280.1"
                     /translation="MTQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            24598..25887
                     /locus_tag="ACG8PO_000937"
     CDS             24598..25887
                     /locus_tag="ACG8PO_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HFQ0206281.1"
                     /translation="MVYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDACRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            25900..28602
                     /locus_tag="ACG8PO_000938"
     CDS             25900..28602
                     /locus_tag="ACG8PO_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HFQ0206282.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            28587..29444
                     /locus_tag="ACG8PO_000939"
     CDS             28587..29444
                     /locus_tag="ACG8PO_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFQ0206283.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(29515..30372)
                     /locus_tag="ACG8PO_000940"
     CDS             complement(29515..30372)
                     /locus_tag="ACG8PO_000940"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFQ0206284.1"
                     /translation="MKITITLPMNHPETTIRDLLEKEWLVPRKVRHFLRIRKNVWINQ
                     QPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDEHLIIVDKPAGMKTH
                     PNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKNPFVLPILGRMLEQK
                     MIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSALTHVTVNKVLSEQT
                     QVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHALELHMIHPFTQEKIV
                     GKALPGLWE"
     gene            30583..32754
                     /locus_tag="ACG8PO_000941"
     CDS             30583..32754
                     /locus_tag="ACG8PO_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HFQ0206285.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPAVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            32822..33163
                     /locus_tag="ACG8PO_000942"
     CDS             32822..33163
                     /locus_tag="ACG8PO_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFQ0206286.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(33439..35193)
                     /locus_tag="ACG8PO_000943"
     CDS             complement(33439..35193)
                     /locus_tag="ACG8PO_000943"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0206287.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLSFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQCISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(35204..36040)
                     /locus_tag="ACG8PO_000944"
     CDS             complement(35204..36040)
                     /locus_tag="ACG8PO_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFQ0206288.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            complement(36042..36725)
                     /locus_tag="ACG8PO_000945"
     CDS             complement(36042..36725)
                     /locus_tag="ACG8PO_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HFQ0206289.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLMPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
BASE COUNT        11868 a         6463 c         7507 g        10954 t
ORIGIN      
        1 aaggagctgt ttttgagtta caagatgcca atggaaccgt tctgcagtca ggtctaacca
       61 caggagcaga tggaaaactg gcgatagatg gcttagcacc aggagcatat cagctggtag
      121 aaactcaagc gccaacgggg tatgaattgg atgcgacacc agtaactttc aagatcgaaa
      181 aagaacaaga agtggcgatc tttttaacaa aagagaatag aaaagttgct gatagctcag
      241 atataagagg aaacaatcca acaagcagtg gaaataagca tcttccaaaa actggagaaa
      301 ccatcattag ccagtttata ttgtcaatat tgggtgtgct acttgttttc atcagtataa
      361 aatttagaaa gaaacgaaat atatgagcta aaataaaaaa ttgaataagc atagttttta
      421 aaatcaacta gtacccatca attgaatgga aactaccgat tttagaaggg gtatacaaac
      481 aaatattagt atcggagtag agaaggttga acccaaccag taaataagta aagacaattt
      541 tgtgctgcgt tgagaagtga aaaataaacc tttgtagtaa tactagttaa taaaaaatat
      601 acaaaaacac tgcgaaaatc attcttgagt ttcgcagtgt tttttgccta atgactcaag
      661 tttattgggt aatactttga ttttctttct acaaataatc agcgatcagt tgtaattttt
      721 tctctagttt taatggctct ttgtcaataa ggagaaaaac aagtcgggtc ttttgtctcg
      781 gaaagcttca accaactcgt gaataaaggt tacgcctcct tgaaaggcgt ggaaaaacca
      841 agtaatcgat caatcaccaa tttcgcattt ggtagaacat gtttggtgag ctgaaagtag
      901 gtggcgttca tgtctgtaac taagaattct atttctttag gattagtgca gctaaggaaa
      961 tagtttgtta atcgaggaat tttttgtgta ggtaaatttt ctcgattaat ttttctgtct
     1021 cgccatccgc ggaaataaaa ctcagtttat cctcaacgga aacgtgagaa cgaaattcac
     1081 aaccctcaac actcaagaca gaattctttt ggaagacttt ggtaaatagc ttctcaactc
     1141 tttcaatgtg cgaataacgg ttgtcaaaga tacctaacaa cttttcgaaa taaaaaataa
     1201 cgacactttt tcagtcagta aagaagtgat tttatgtctg acatgatttg aaatcgagtg
     1261 ccttggacgg ataaaataac tttgagcgtt ccaatggctt cgacagtttt tacaggtata
     1321 gcgctgcttt tttaggcgta taaccattgg catatgatta tattattcaa agtggacaat
     1381 ggtttccttc ttcttgtttt tcaccaccat taatttccta tttccatcta ccacagcaga
     1441 accacaattt ctacagacac atggagcagg gaagagaact gcagcaacga tcaatgtttt
     1501 tttcttctgt aaggtttcgt aagagacctc tgtaatcatc aaatttttgt ctattgatct
     1561 cagaattttt ttgatagaac cattcataaa gcatatcgtt ctctcagttg tttattttgt
     1621 ggtgatttaa tcatacaaga aaacgatatg tttttttata aaaacatgaa taggaaaaat
     1681 agttgacaag aaaatgacaa cgccatatga tgttccatag tatttacaac ctgtcataga
     1741 tgactgtaaa aaataaaagt aaaaagaaag gacgacaaca ggtggatttt tatcagtctc
     1801 tacaactaga tccgtttatt ttaaaacaaa aaatacgaga agcagcttct aaaaaagaaa
     1861 agtgcatgta tatttgttca ttgtttttac gatcattatt catcgtattg tttgccattt
     1921 gttttatcat tataattaca acattatttg aatcgaagca taaaccatac gctgtggttt
     1981 tattctgtat gttgatgagt attcgttttg ttgactttgg ttataagatt tcccattcga
     2041 tcataggttt agcaatcgtc atgttctcac tattagtagc accgtatgtc cagctgatca
     2101 aatggtcagt tatgggcatg ttgattcatt ttattttact aagcagcata ttaatggcaa
     2161 ccgctagtga tcccaaaatg gggaatgctt ctttgtacgg cttttcatat ctctttattg
     2221 tttattcgtt acctaaagat tcgttgaaca agaatttctt tacacaaaca agtagcttat
     2281 tatttttgtt tttttgttgg ttttcagttc tcctttacag aaaacatcga gaaaagaaca
     2341 aagacaaaag cttgtttaaa gagatttttt tgaaaggcat gtattcgcag gaaaaaattt
     2401 ggatgctcat ctatgcattt ggcattagtc ttttgatcgt tgcaggagag tatgtccctt
     2461 ttcagcgatt gatgtgggca gggtttgcat tttcttctat tgtatctagc tatggattga
     2521 tgtcgattgg tttcaaagaa cgagcagtag accgaatcat tggctcattg attggatgtg
     2581 ttttatttat tggtatcagc caatttattc cttttaactg ggtcggtata ttaggtggtc
     2641 tagctttagg tgtttgttct acctatagat ataaaacagt ttttaattct tttggtgctt
     2701 tggcaatcac tgccagttta ttcggggttc caggagcagt aacgatccgc atttttgaga
     2761 atatccttgg ggtatgttta ggaatcatgt atattggggt gactgaaata cttatccgaa
     2821 aaataagaga aaaacacggt ttgaatcatt agatgagatc gaataaaaaa taaggaaaaa
     2881 gtgaagatac acatggatga taaattgcta tcattccatg tgtatctttt tacggtaaat
     2941 cagtgaaacg ttcgactttc atttttaatt tatacttttt acggagttcg tttatttgag
     3001 ccatcatttc ggatttgtga tgcttttcga ttgcttcttc atctttccaa cggtcgatca
     3061 gtaaaagaga ttctggatct tctagtggaa agtaatagtc atatttctcg tttccttctt
     3121 ctgcacgaat cgctttgaca atgtttcttt cgaccatttc ttctgcaaat tttctggctt
     3181 gtccatttgt acttgtatac aaaatattca ctgtgatact cactaaatcc cctcctgaaa
     3241 aaattctatc actagtatat cacggtgcca gaaattatta ttcaagatat gatacacatt
     3301 ttttccgcaa tgctacggaa atatatattt tgaatagaaa acgcttacaa gatatgcttg
     3361 gtttataaaa ataaaaggag tgattgattt atgaaaaaag cattgattat ttgtgcggca
     3421 ggaatgtcat cctcgatgat ggcctcaaag acaacggatt tctttaagca aaaaagagaa
     3481 gaggtactcg tagatgcagt ttcagctaca gaaggcgata atatgattaa gaatagtgat
     3541 tttgatttat ttctgatcag cccgcaaacg acgatgtttt tggataaatt caaaaaatta
     3601 ggagaatctg ttggaaagcc tgttgttagt atccctttcc aagcgtatgt cccaatccct
     3661 acaggcatcc agaagttagc agaggtgatc gaagagaaca tatgatggaa taggagtgga
     3721 tgataatgaa acagaaggaa aaattgctgt tgagatattt gattgatcac aagaaggagt
     3781 atgtcaccag tcaacgttta gcttctgaat tgttattatc tgatcggaca atccgtaatt
     3841 acttgcagcg gatcaaagag ttagtagaaa aaaacggagg taagatcatt gctaaaccag
     3901 gttatggcta ccaactgcat attttacaaa gactgacatt tgacttgttt ttgagtcggc
     3961 aagaaatagt acctggatat gctagagaac cgcaaggatt ttatgagtcc gaagatcgac
     4021 aaaagtatat attgaaccaa ttgcttttgg aagatgccat tctttttatg gatgatttgg
     4081 cagaagagct ttatatcagt cgttccagtt tgacaaagga catgcaagaa atcaaggagc
     4141 gattgattcc atattcacta aaaatcgttt ctaagcatgg tcaagggaca tggatcgaag
     4201 gggaagaaag aaatcgacgg cattttatca tggatacttt ttttggaaac cattatggaa
     4261 attcattgaa agaatattta gggcaaagcc gttttttccc agacatcagt tttgaagagc
     4321 tagtcattat cattctcgat gaaacaagag aagctaagtt aaaagtttcg gattttatca
     4381 tacagaatct gactttacac ttggctttgg ggatcaaacg gctccgagaa gggtttgaaa
     4441 tcaaggaatt aggcatcgaa caagaagttt ctaagcgagt agagtatcaa gtggcgcagc
     4501 ggatcgttca acgtattgag gtaatcgcag gtgtatcgtt tcccgaagaa gaaattgctt
     4561 atttgacatt acatctcatg gctaagtcga atcacaaaga aaatcaaaat gatcaggaac
     4621 tatcaaagga attagcgaaa gttctgaaaa gattgtctca ggaatttcat ttttcattag
     4681 tagaggatct ccagcttttc aatggactac tcgatcattt gtcaccgatg atgattcgat
     4741 tgagccgagg tattcgcatt gagaacccat tgactgagga gataaagaaa gaaaatccca
     4801 aagtctttga cgcagtaaaa aggcatttca gcaacatgcc ggctttgaaa aactatacca
     4861 tcaatgaaga tgaatgggcc tatttggctt tgcatctcat ggcggcattg gaaaaagaaa
     4921 gagctgcgca taaactacat gcattgatta tctgtgcaac gggttacggc agtgcccaac
     4981 tattaaagaa tcgcgtagtc agtgaattcg gtaaaaatat tacagtggta agtgtaaaag
     5041 gctattatga gatcaatgaa acaactttaa aaggaatcga cctgattatt tcttctattg
     5101 atctttcgac aatgtttttc aaaattccag ttttacatgt cagtattttt ttgaatgaag
     5161 atgatgttcg taaaatacga aaggtagttg gagaatctat accctatcat tcctcagata
     5221 gaccgatacc agcattttct ctacagcaaa aaaagcagat ttacaccgaa cagttgtctg
     5281 aaaaattttt caagacttat acgtacgaac cgaaaaagga agaaattctt catgatttgc
     5341 tgcaaaagct gagcataaat gaagaagaag gatatataag agaaatgcga aagcagatcc
     5401 agcaacgtga aaatatggga caaatcatat tcagtgatac agtggcagtc ccacatcccg
     5461 ctgttccagt tggaatcgaa acaaaggtag ccgttgcatt gattccttct gggatgcagt
     5521 gggatcaata tcaagaaatt cattttgttt ttttagtttc gccttcttat gtcgaaaatg
     5581 aaggaattac ggtcgtgact aaagcaatcg ttaggttagt tgaccagcta aaagtacagc
     5641 aggaaatctt agctgaacct atttttgaga atttcagcag taaatttatc aaactgatcg
     5701 aataaggagg aaaacagatg gctgaaaaaa taaacagtga aacactacaa gtgacttctt
     5761 ttgatatcat tttccatagc ggaaatgcac ggacactgat acatgaagca tttcgctcga
     5821 tgcgtaaaga gatgtttgaa gaagtagaag agaagttgcg acaagcaaat gaagagattc
     5881 ttcatgcgca tcagtcacaa actcaacttc tgaaagaata tgccagcggg acaaaaatag
     5941 aaatggaaat tatcatggtc catgcacaag atcatctgat gacgacgatg acattattgg
     6001 aagttgcaca ggaaatgaat tatttgtatc aaaaaaataa atagaaagga aggatcattg
     6061 tgcaggatac agtaaaaagg aaaatcgaat tagaaaaaaa gcaacttagt ttgaaaaaca
     6121 tgtatttcaa tcgttatcta atcgtacgtt acctgactgc ctttttcttt ttcatgaaca
     6181 tgcaatggat gattctgctt ctatcagcta aaagtttagg aagtagttta ccgatgattc
     6241 tgattttagc tattttgcct gcagtaggag aacaagtaaa gctttatcga aaacaccaaa
     6301 cgaacgttcc atggacaaag tggtattttt tgttccagag tgtctttaat ttattagtga
     6361 ttccagtgtt gtttacctca ggatttacat tcttgtatcc ttttatggca aataacaatg
     6421 gaggacagtt atttgttttc attcttgttg taagcggtat ttttgccagt gcatttatac
     6481 agtatcgttt aaagaaaatt tcactcaacc aagaccaaca gtatatccgt attaaacaat
     6541 atgaaaaagc gttatattta ggaaaggaga ataactaatg tttgattttt tgcagaaata
     6601 cctgatggga cctatgggga aaattgcgca acttaaaatc gttcgggctg tcatggcagc
     6661 ggggatggct tctattcctt tcacgattgt aggctcgatg tttttggttt taaatatttt
     6721 gccattaccg ttcccatttt tagaaggatt ttttaatgca acttttttca aagttagtga
     6781 cttgtacatg atcgtcaata caatgacgat ggggatttta tctatttact ttgccattgt
     6841 gtttgcttat gaattaacgt caattgaacg agatgaacaa aaattaaatg ttaatccgct
     6901 cacgggggca ttactatctg tttttgcatt ctttatgtgt atccctgaat tgatcatgac
     6961 tgatggtaaa atcagcttga ttgcttctat caatgagaca gaaactgtag taaatggtat
     7021 taggatgggc tcttttgtcg aacgtttagg aacttcagga atctttacag ctattattat
     7081 gtcgattatt gcggtcgaat tgtattgtat gtgtgtcaag agaaattggg tcataaaaat
     7141 gccagatgtt gttccgccag gagtttctcg ttctttcact gctctgatcc caacatttgt
     7201 cattgcgttt gtggtgatga tcatcaatgg attattagtt gcactaggaa cggatatctt
     7261 caaaatgatt gctatcccat ttgggtttgt tacgcagttg acgaatacat ggcttggtgt
     7321 catggtgatt tatttcttga tccatgcatt gtggatcgta gggatccatg gagcgaatat
     7381 catcacttct ttcttgaccc cgatcgtatt agctaatatg gcagctaacg cacaaggcgc
     7441 aaattatccg ttggcaggag aattcaacaa ttcttatgta accgttggtg gatcaggtgc
     7501 gactttagga ttgatcatct ttattgcatt cctcgcgaaa tctgatcagc tgaaagtgtt
     7561 aggaaaagcc tcacttgtac caggattatt caatatcaat gaaccaatca ttttcgggat
     7621 gccaatcgtc tacaatccct atttagcggt tccgtttttc ttagctccga tggcttctgc
     7681 tacactcgct tattttgcca ttcggctgga aatcgtcaaa ccgatgcttg cacagatgcc
     7741 gtggccttca ccagtcggga tcggagcatt tgttggtagt ggtggtgatt ggaaagctgc
     7801 tgtgttagca gtcatttgtg caatcatcgc attcattatc tggttcccat ttatcaaatt
     7861 ctatgataat aaattattaa atgaagaaag agaaaaggca gcagaattag ctagtgaaaa
     7921 gttaaaatga tttctaagaa aaaaggaact gtgacaaaaa atcacagtcc ctttttttct
     7981 tagaaatggg aaaccactag tatataaata ccattattct tcaatcaacc gcttcacgat
     8041 tgcataatcc ccttcataag gttcatctac accattgatt ttttgatagg gatccacgcc
     8101 ttttcccgca ataacgactg aatcttgcgg agcgctttct gctaaagcgc gtcgaatcgc
     8161 ttcaggacga tccatttcat aatgaacgat caaatctgga ttagtaattg cttcattgat
     8221 ttcttccgcg atttttttcg gatcttcaaa tgctgggtca tctgccgtca aaaaggcgat
     8281 gtcagctgtt tctgatagca cttttccaaa atcatgtcga cgggagattg ctttgtttcc
     8341 tgggctgcct aatacaacaa ttacacggcc atctggatgt tcatttttgg caaattccag
     8401 caatgttttc aagcttagat aattatgtgc atagtccaca tagacatgcg ctccattgga
     8461 ttggatcaat tgatccattc ggcctggtac acgagcttct tttaatccgt cttgtgcatc
     8521 ttgactggag gcacctgtta gtgctgcggc aatgatcgca ctggctgcat tttcttggtt
     8581 gaatcctcca gccagcaaaa tatcataagc agtatcgcag atagctaagc gatcttcatg
     8641 agaagataaa gtaaaaccgt gagtaccttt atctgaacgg atcacttgat aattgctttc
     8701 ttccgaacga ccataagtga tcgttggaat ctggaataga tcggctgttt ccctaagtaa
     8761 atcaaaatag tcactttcat gatttaaaac aactgttttt gaattcaaga tcaattgtcg
     8821 cttgcaatag aaatagtcat caaatgtcgg atgttcgatc ggactgatat ggtcaggcga
     8881 gatattcaag aagattccga catctaaagt caagccgtat acgcgttgtg ttttgtaagc
     8941 ttgtgaagaa acttccatca ctagatgagt cataccattt tcaactgctt ctgacatcat
     9001 acgatataaa tctaatgact caggcgttgt caaatgggat ttgaaatatg ttttgccatc
     9061 taacgttgta ttcatagtag aaagcaatgc tgttttttta tttgttgtgt gatccaagat
     9121 tgcttttgta aagtacgcag cagttgtttt accttttgtt ccagtgaatc cgatcacttt
     9181 caatttattt tgcggatagt cgtaaaaagc catagcaaga agcgccattg ctttacggat
     9241 atccgttacg atgattccta ctgctgaatt ttcgtatggt tgttctgata cataatattg
     9301 aataccctgt tcgattgctg aatccaaata ttcttctttg aaattcattc ctttacagaa
     9361 aaacaaagta gaagcatcag cttttcttga atcataactt aactgaagaa attcagcttt
     9421 ttctggtaca tcaagatgcc agcctcttgt tgaaacaaat tcttttaata gattttcttt
     9481 taatagtaaa tgacggatgt cttctaaaga gaagctcatt gattgatccc tcgctttatt
     9541 caatttcttg gtttattata gcacactctt tttaatatat cgtttgtttc cgctatgaca
     9601 aaatccagct ttattcaaat tttcttgaca caggggacaa gcggatgagt attttcataa
     9661 acaatgaact gactgtaata gataggagaa cgaatagtgt ctccaacaat ccaaaaacat
     9721 aataattcaa aaagacgata agactattgg taagtaaaag tccttgcggc attggaagcc
     9781 gaaaaaattt gtggaaaata atcggcggta tcgttatacc gccatttgag gccccgactg
     9841 tgtacaatgt gtaaacccca agactgaaca aaagacttcc tgaaatcagt gaaaagaaat
     9901 gtgaggagat catttccact tttggaacaa tctctaaaac aacaggaaaa agcagacttc
     9961 ccgctacagt cttaaaaaat actgattttc ctaataggac agcggctaga agcagcatca
    10021 taagattgat gctccaaaca gtaactgagc gttggataga aaaggccgtt tcaactaaat
    10081 gtccgatggc gcccactccg gcggaagcta tatcgtgagg gagcaagaac caattcaatc
    10141 caactgtgat aaagaaaaga cctaaaaata taacgggtag tttttttatt tcttgtgtca
    10201 ttgatatctc ctataaatgt tagccataaa aggctgtata tttctttact ttaccaaaaa
    10261 aacgaaaccg aattcaaaaa aacaattgtt cttataaaag ttatataaaa tcgattgcca
    10321 gtttctgttt taccaattaa aatgatagta atgttttgta gaaagttgga atctgaccga
    10381 tggataatca aagaaaacca gcattagatc aattaacacc gcaagaaaaa atggcacgag
    10441 gctctgcttg gatgaccgcc agtaatatca tttcaagatt gctaggcgct gtctatatca
    10501 ttccctggta tgcttggatg ggcgaaaatg ctaaggctgc caatggatta ttcaatatgg
    10561 gatacaatat atatgcattg tttctactga tctctacagc aggaatacct gctgcgattg
    10621 caaaacaaac tgcacgctat aattcattga atgaatatgg cacttcacga cgattattta
    10681 tccgtgcatt acagatgatg ggcgggctag gcctgttgtt tgctttgttc atgtacatcg
    10741 cctctccttg gctagctcat gcttctggtg gcggagaaga actgattcca acgatgcgtt
    10801 ctttgagtat cgctgtattg gtttttcctt gtatgagtgt gatacgaggg tattttcaag
    10861 gcaatcaaga aatgatgcct tatgcgctgt cgcagattgt agaacaggtg gcacgtattt
    10921 tttatatgct attggctaca ttcatcatca tgaaagtcat ggatggtgac tatgtgacgg
    10981 cggtcacgca atcaactttc gcggcattta tcggtatgct agcaagtatt cttgtccttt
    11041 tgtatttttt gaagaaacat caagcgtata catcggcgtt tattcactac agcgaaaaca
    11101 aaataaatat tgcaacgaag gaattgttgt tagataccgt taaagaagcg attccgttta
    11161 ttattgtggg ttcaggggta acgatcttca aattagtcga tcagttcact tttatgaaga
    11221 tcatgtctgc atccaccgag tattccaatg cacagttact ggatcttttt tcgattttta
    11281 gtgccaatcc agataagttg acgatggtag ttattgcatt ggcaacatcg atcgcgtcta
    11341 ctgggttacc gttgattaca gaagctgtta cgttgaaaga tcgagtaggt ctggcaaaat
    11401 tgacaagcgg aaatttacag ttgttttcat tttttatgtt tccggcgaca tttggtgtta
    11461 tgttacttgc ctatccgcta aatacattat tttatacacc ggattctcta ggaagtaatg
    11521 tactgatcca agcaagtttt gtaggactct tcctaggact atatatgcta gtttccaaca
    11581 tgctgcaagg aatgtttgaa aacaaggcgg caattaatta tttggttatt gggtttctag
    11641 tgaaactcct tcttcaatac ccagccattc gtttatttga agtatacggc cctttattgg
    11701 cgacaatgat cggatttgct gtgtcttgta ccttgatctt aaagagaatc catgaagtag
    11761 cccgctttaa accaagtttt gtctggcgca ggacattgct gatctttatt cttaccttga
    11821 ttatgctttt ggcttcgtgg atcacaaaga tgatttttgg tgctttttta aacgaagata
    11881 gcaaattcca gtctctattg ttgattcttg tagtagcagg tataggggct ttagtgtatg
    11941 gttatttagc tttgaaaatc cgcttggcag aaagactact aggaccagga atagcgcgtt
    12001 tacgacataa attaaaaata aaataagtga atcagaagag ctagattgat gctcttttga
    12061 aatgggggaa acgcatgcgt ttagataagt ttttagcaga tatgggactt ggcagccgca
    12121 aagaagtcaa aggattgatc aaaaaaggaa cgatcgaagt caatggacaa gtggtgaaaa
    12181 gcgataaagc gcaagtaaat gaattcgaag atgcggttac ttatttagga gaaatcatta
    12241 cgtatcaaaa agatttttac tacattttgc ataaacctgc aggagtcatt tctgctactc
    12301 aggataatca tgatcaaacc gtaatcgatt tattggaaga ccaggattat cgggaagatc
    12361 tttttccagt cggccgatta gataaagaca cagagggcct cttgatttta acgaatgacg
    12421 gggtgtttgc ccatcagtta ctatcaccta aaaaacatgt ggagaaagaa tatctagctg
    12481 aggttagcgg tataatgaca gaagaagata tccgtttatt tgcagaagga ctgattattg
    12541 atggcgatga gcagacattg ccggcaaaac tccaaattga tcaagtagat caagagacag
    12601 aaacttcaat tatccgtctg attttgcatg aaggaaagtt tcaccaagtt aagcgaatgg
    12661 tcaaagcagt aggaaaagaa gtcttatatt taaaaagaat ccgtatggga aaattttggc
    12721 tgcctaaaga tttgaataaa gggcaatatc gacccatgac caaagaagag ctagaacaag
    12781 tgagagaaaa ataattgaat ggatagctaa aagaataagc agagtagttg tagcgccatc
    12841 tgaaaagcag acttgtccgt ctgactttcg gatggctttt ttgtgaaatt tcggcttatt
    12901 tccgtagaag ctacttctgg aacaccgttt gttcggaata agggactgtg acaagacttt
    12961 tgttacagtc cccattgttc cttgtgactc agagttagat agtaaggaca cgatctccct
    13021 aatactcagg ccaaaacgct gaattcacaa cctctgataa acggtgttca atcagctgac
    13081 cctcggtatt agctcaaaaa acggcaaaat atgaggaact attttcccgt ttttttctct
    13141 aatactcagg ccaaaacgct gatttcacaa cctctcctat actttgtttc attttcccta
    13201 tactttgttt atttattctc attattgaaa gcgctataat tacgttgcga atgaatcgaa
    13261 aggggttttt agatgaaaaa acgaaagggg ttcaatcatt tttttatgaa tctatggcgt
    13321 tataaagcac ttgtcctgat ggctgtaccg ggtatgcttt ggatgatttt tttcttttac
    13381 atacctgtaa tggcgaatgt cgtagcattc aaaaattttc atatttcagc agatgggttc
    13441 ttggctagct tgagggaaag tccttgggtg ggttgggaaa atttcaaatt tttattttct
    13501 tcggatcaag catttctcat cactaaaaat acaattttat ataatgtagc atttatcctt
    13561 ttgaatctgc tgatctcagt cgtatttgca atcattatga gcgagttgag aaataaacga
    13621 ctcgttaaag tttatcaaac gatgtcctta ctaccttatt ttctatcttg ggtcattatc
    13681 agttactttg tttacgcttt tttaagtcct gacaaaggaa tattcaatca gtggatcact
    13741 ggacatggag gagaagctgt caattggtat aatgaaccga aatattggcc gtttatcttg
    13801 gtgttcattg gtacatggaa agggattggt tataacagca ttatttactt tgcatctgtt
    13861 atgggaatcg atccgactta ttatgaagct gctatggttg atggagctag caaatggcag
    13921 cagatcaaac atgtgacgat accacaactt attccactaa tgacgatttt aacgatttta
    13981 gccgtaggaa acattttccg agctgatttt ggcctgtttt ataacattcc taggaattcc
    14041 ggtgcattat atgatgtgac tcaagttttg gatacgtata tctacaatgg cctgacatcc
    14101 acaggagatt ttggtatgac ggctgctgca ggactttacc aatcagttgt cggctttgtc
    14161 ttgttgatga tcacaaatac gatcgcccga cgttttgata gcgattctgc attattctaa
    14221 ggaggaaaaa aatgaaaaaa aagaaagtgg agagagtcca agtccgctct ttcaataaga
    14281 cggctaattt tttctctaat ataatgattg cactctttgc tatttcctgt attttgccat
    14341 ttatatttgt tatcgccatc tcttttaccg atgagtccat attgtcgact cacgggtaca
    14401 gtttttggcc agaaaagttc agtatatttg gttatacgta cttgttcgac cagatgcagg
    14461 ataaaatttt ccaagcgctt ttcgtcactg tacttgttac tgttttaggt acactgatca
    14521 atagcacggc tacttcttta tatgcttatg cgatttcacg gtcaaacttt ccattccgtc
    14581 gtttttttac cgttttttgt ttgattacga tgttgttctc accagggatg gtggccaatt
    14641 atcttgttat gacaaatctt cttcagttaa aagatacgat ttgggcattg atcttgccaa
    14701 tggctgtcag tcccttcaat attattgtga tgcgcacatt ctttaaacgt tcggtctctg
    14761 attcgattat tgaatcggca cggatcgatg gagctagcga actgcgaata tttgttcaga
    14821 tcgttttacc gttagctgtt ccaggtattg cgacaatcag tttatttgcc gcattgggtt
    14881 actggaatga ctggttcaat gctttactat acatccaaga tgataagcta gttccactac
    14941 agtatctttt gatgaaaatc caaagtaata ttcaatatct gacacaaaat tcaggagcag
    15001 gaagccaatt gacaggcgga ttagcctcag ttccaggaga atctgccaga atggcgatcg
    15061 tagtcatctc gacgttgcct attgcgatca gctatccatt tttccagaag cattttgtca
    15121 aaggtctaac aatcggcgga gtaaaagaat aaagaaacaa aaatataaat cataaaatta
    15181 ggaggaagaa aaatgaagtt atggaagaaa ctagccttta caggtgtttc agcgttgttg
    15241 ttgggaacat tagcagcatg cggaggttct ggttctaaag accaggcaga agctagcaat
    15301 tcggacactt tgcagatgta tcaaatcgga gataaaccag ataatttcga tcaattgatg
    15361 gaagtagcaa acaaacgaat cgaaaaagag atcggtgtaa aagtcaatat caattacatt
    15421 ggctggggcg attacgaaaa gaaaatgaac gtcatcattt cttccgggga aaattatgat
    15481 atcgcctttg cgaacaacta tgtttccaat gcccaaaaag gagcctttgc tgatttgact
    15541 gaattagcgc ctaagtacgc tgaaaaagct tataatgatt tagatgaagc atatattaaa
    15601 ggaaatctgg tcaatggaaa attatatgct ttcccagtca atggaaatgt ctttgctcaa
    15661 caagtactta cttttaacaa agctttacta gataaatacg atctttcaat tgatggaatc
    15721 caatcttacg ctgatgcaga aaaagtcttg aaggaattcc atgaaaaaga accaaacacg
    15781 gcagcttttg ccattggaca aggcttcaaa gtccaaggga actttgatta tcctttaggg
    15841 aatacgttac catttgctat tgatctggat ggagatgatt cgaaaatcat caaccaatac
    15901 gataatgaaa cgtatatcgg actgttacgt acaatgcata gctggtacaa acaaggactc
    15961 attccaagtg acgctgcaac gagcaataag gattatccac tagaaggaaa tacttggcta
    16021 atgcgtggag aaacacaagg accgtatgat tatggcgata caattttgac aaatgcagca
    16081 aaacaagaac ttgtctcaaa agcaattact gttccactaa aatctacagc tcaagcacaa
    16141 atggcaaatt ttgtcgtatc aaatacttca aaaaataaag aaaaatcagt agaacttcta
    16201 gggctgctta acagtgatcc agaattattg aatgggcttg tttgggggat cgagggagaa
    16261 gcatgggaaa aagtcgatgg ttcagatgat aaaatcaaat tgctagatgg ttatcagcca
    16321 aatacacata tgtctgcttg gaatacagga aacaacaaga ttctatatac acaagagtct
    16381 attacggatg acatgattgc caaacgtgat caatcgattg ctgatgcaga aacttcaccg
    16441 attttaggat tcagcttcaa cactgacagt gtcaaaacag aattaagtaa tatttctaat
    16501 gtcatgaatc aatatttaga tggattgaat acagggactg ttgatccaga tgaaacatta
    16561 ccgaaattga aagacgcttt ggacaaggct ggctatgata aggtattgaa agaaatgcaa
    16621 aaacaatatg atgagttccg acaagaataa taatgaataa agacaaaggg gttgtgacaa
    16681 aagacgtgtc acaacctctt gttccgaata aacggtgttc aaaagccagt ttctcggtat
    16741 taagctaaat atatgtaaaa atgtggaaag ccatattcac aaaatttttc ttactactcg
    16801 aaactaagcg gctatttcac aaactcttgg taaacggtgt tcaatcagct gaccctcggt
    16861 attcgctcac aaaacgacaa aatatgagga actatttttc cgtttgtttc tctaataatc
    16921 aggtcaaggt gctgattgcc caaccttttt ttgtataatg agaaagtata aacttaatgt
    16981 ttggaggaga agatatgtta gggaaggttt tggaagagtt atttatccgg atctgggtca
    17041 ttataaaact aaccttgtat ttctggatat atacatttgc cggcgggatc atctttggat
    17101 taggtgcggc atggaaaaca gtcaatgaac tattttattt gtacggattc gagtataaag
    17161 aaatcacaat aaaaagaggc tggaatatat ataaaagaaa ttttttaaga ggcaatctgc
    17221 tatttagttt atttttatct ggtactgctt tattgagtta caatttatat ttatctgttc
    17281 aaatcaaagg gctcattttt ttagctattg attttatttt actttttgcc ttgctctgcc
    17341 tatttgctac ttatcaatac agtatgattt tagatagtga atatacgatc tctgttccta
    17401 atctgctaaa gctaagtttt atttcggttt tttccagctt ttcgtctttt ctaaaaataa
    17461 ttattggtag cggtataatt ttaggcgtga cttggcaatt caaaggattg atcctgtttg
    17521 gtgtcattgg tcttttgacg gtttggaatg gtacgatgac aacacattgg cgtgaagaat
    17581 tagataaaca gttggaaagt tatgaataag tttcggattc tattttcaaa aggaacctta
    17641 ctaaatcgtc tgttgcggaa atacgcggtt atcatgatcg gtgtaacgat gactgctacc
    17701 gttattttta gtatccatac ttgggaacaa aatcaaaaac aggcagaaaa catgacaagt
    17761 gatgccgttc aaagcacaag cagaatgctg aatgataaaa caaccttgag taggatcatc
    17821 aaaaaccagc ttgtagggaa ttccgaaaag atcgaaaatg tgacgaccta cttaacaaaa
    17881 cccatagatc agtatctgat gtatgtatac gaacagcaaa acagtaccga tgaattagtt
    17941 tctttcccaa atcaaatcaa agatctctac gcaaattatg aagaactaag tgcgatatat
    18001 atcgtgttga atcaactacc ggagtattat gaatcttcta gagaaaacaa aggtggggaa
    18061 ataaaaaagg ggacaccaaa attactagac gccttttata tcaagttgcc tatcacacaa
    18121 ggaggagctg agtcaggaag tatttttatt ggttttcaaa agaaagaact agatatgatt
    18181 ttagaaaatc taacgtcatt caatggactc tctttataca tgatttctgg aacgacaaat
    18241 cgactataca cattccatga taataaaata gctaaagaaa cgtttgaaag acaagaaatg
    18301 atcatcacag agagtctgaa gaaaaattca caattgccga ttgacgatct ggaaaaaaac
    18361 aattttgttc aacaccaaga actgtctgat gattatcgta tattagctgt actagataaa
    18421 aaaagtgtaa aaatcgaaac gggaaaaaat cttgccccgc tgatcattgg tatcattatt
    18481 ttagacaata tactgcttat ctttctttat aaaacattca aacgatattc acagcaagta
    18541 tctatcgtca tgcaggccat ggatcaaaca gcagcaggga atttagagac gcggatcgac
    18601 ataacaaaca cggaatatga actgaaggaa ctttcaatcg gtatcaacga aatgttggat
    18661 agcatcaatc agtttgtaga agacatctac aaattagaaa tcaaacagca agacgcacat
    18721 atgcgggcgc ttcaagcaca gatcaatcca cactttttat ataatacatt agaatatatc
    18781 cgaatgtatg caatcagcga aggaagtgaa gaattagcag atgttgtgta tgcgttttcc
    18841 gctttgcttc gaaacaatac gaatcaagag aaaacgatca ctttaaaaga agaactggac
    18901 ttttgcgaaa aatatgtcta tttgtatcaa atgcgttatc ctaatcgagt agcctatcat
    18961 tttatgatcg atcctgattt ggaaaagatc gaagtaccta aatttgtgat ccagccatta
    19021 gttgaaaatt attttaaaca cggtattgat tttactcgat tcgataatgc gctaagtgtc
    19081 aaagtcttgc aggaaggcaa acgggtaagg atcatcatca aagataatgg aaaagggatg
    19141 actgaaaaac ggctaaaaca gattgaagaa aaactgtctc atcctaaagt ggaacttcac
    19201 ggttctatcg gtctgcaaaa cgtgaatgaa cgtttacgtg ccagcttcgg cagcagttat
    19261 tatatgtcgc ttgagaataa tgagacaggt ggactgaccg tttctattac attcaaggag
    19321 ggatgagcgt gtatcaagtg ttattggtag atgatgaata catgattttg aatgggttaa
    19381 aaaagataat tgattggcaa agtttaggct ttcaaatagt agcaacagca gagaatgcca
    19441 aagaaggact agctgtactt gaacagcgac agattgattt agtagtaacg gatgtgacaa
    19501 tgccagaaat aaatggactg gaatttattg aagcggctca aaaagaacgt cataattttg
    19561 aatttatgat tttatcagga taccaagaat tcgattattt gaaaggaggt atgcaattag
    19621 gcgccgtgaa ttatttgatg aaacccgtga ataagttcga attggttgaa agcctcaaga
    19681 aaatcaaaac acggttagat cagcaaaatg aacaaaaaaa tcaacaagaa atctatcaag
    19741 aactgttatt cagtcaatgg ttgaatgaag aattggatga aataagtgaa gaagaaataa
    19801 taggacaaat aagtgagaag cagcatagga tagtattgat tcaactggca agaactcatg
    19861 atactcttat caatagatgg ttgcaagaac ggcaacaaat cttttactat caacgaaatt
    19921 acggagaccg tgtattgatc acgttgttgt tggaggcaga tgaagttgat ggcttctgtt
    19981 gttttgttca agaaaacttt cctgatcagg aatggctgat cagtatcggg gaagagacag
    20041 attctttgga caaaatccct gaaacattcc aacaagcgaa agacaatcta cagttgcatc
    20101 aattttatgg agataaagac gagcatgttt tttacgcagt acaagcagaa acaaaagatc
    20161 aaaccatcga tttttcaagt ttcagacgcg tgctgcaaaa taaacaattg gaagtcgctc
    20221 aaaaaatgat cactgatttt tttgaacagt ttcagttagc agccatgatg ccggaagata
    20281 tacgatattc cagtttttta ctgtttatgg agattcaacg ggaattgatt gatctggaag
    20341 atgaggaata tttacaagga atcgaaaaaa tccatcaggc aaaaacggtg caagaacttc
    20401 agcagctact tctgtcattc atcaaaaagc atcagagaca gaaaaagtac agtgacaatg
    20461 tggaaaaagt aatcgtaatc ctgcaccagc attatcaaga accacttaca ctcaaagaag
    20521 tgtcagaaaa cctgcattta aatgtcatgt atctaggaca gctgttcaaa aaagaaacga
    20581 aaaaaagttt ttcagcttat ttgaaccatt tacgtatgga aaaagcgaaa caactgcttt
    20641 tgcacagtaa tcaaaacatc aatgagatag caagtgagat tggctacaac aacaccacgt
    20701 atttctctaa attattcaag aaaatcgtcg gcagatcccc taaagagtac cgagaaaaca
    20761 gtggagaatc ataaaaaaaa aagccaatgt aatgaaataa cttataaaaa attgtctaga
    20821 aatctaattt tgggctgttc ttattacttg aggcaaggca gctttttcat gacctctcga
    20881 taaacggagt tcaaaagcca gtttctcggt attaagccaa atttatgcaa aaatgtggaa
    20941 agccattttc acaaaatttt tcttaatact cgaaactaaa cagctttttc acgacctctc
    21001 cactaaatgg tgttcaatca gctgaccctc ggtattagct caaaaaatgg caaaatatga
    21061 ggaactattt tgccatttgt ctctctaata ctcaggtcag aacgctgatt tcacaacctc
    21121 tcaataaacg gtgttcaaaa gcttgcatct gcggtaataa gaccaaccaa acgaaaaata
    21181 tgaggatcta tttttgcttg gctgttctta ttacttgatg caggacagct ttttcacaac
    21241 ctcttgttaa ttatagtttg ataaggaaaa aggcaaatct tttgcttgat atttcttagg
    21301 gtgagggatg atccctaatt ggtaagagat cgtgccgcct ttcagcaaat cttgatgggt
    21361 aaaatataat ttcgtatatt ctctgtccat ccgtttgatt ttatggataa attgttgctg
    21421 cggccgatta ggcgttgctt caatcgtcaa ttcctttcca tttgacaaaa gcaaaacagc
    21481 cttgtcaatt aatggcatac cgataacata ttcaccggtt ccaggcgtga caggataaaa
    21541 tcccagacta ttgaagatat accaagcggc catacttcca ttgtcctcat ctccagggta
    21601 acccattgga gaatcgtcga aggtctgagt catcaactgt ttaagcagtg gctgtgccat
    21661 ttcaggttgt cctatataac tgaacaagta aggatagtga aaacttggct ggtttgaaat
    21721 agctaattgt ccaaattcta tcgcggccat ctcactcatt tcgtggatct caaaaccata
    21781 tcctcctggg tcaaacgttg ggggctgatt gcacagctca atgagttttt ctttatatct
    21841 ctcactgcca ccatattttc cgatcagtcc tgaaaaatcg tgataaacag caaagctgct
    21901 ttgccaagca cttccttctg cataatcttg tccccaacga ttagctgaaa atggttctct
    21961 gaacttgcca tctttatctt ttgaacgcat aaatcctgac tcatagtcaa agatattttg
    22021 atagttcaat gattgctgct gatatttctt cgtcatttcc tcttcttcta gtaccttggc
    22081 aacttggcta atacaaaaat cgctataagc atagtctaat gtgtgattga ctgattcgtg
    22141 gtagtgatta ggaacatagc catatgtcaa gtaatcttct gttccttggc gtccataatt
    22201 tggatgtttg ctttgaaccg tagcaccttt ttccattgcc gttagaaatt caggcatcaa
    22261 gtctcgtcgg atatctttta cagcagcatc tgcaatgacc gcatcgatca atgtcccagg
    22321 cataagtcca cgttcatcag gagacagcca ttttggcaaa tagccacttt ctctatagct
    22381 gttcaaaaaa ccttccagca tttcttcgta tttttctaca gcaatcaaag aataaagggg
    22441 ataaaccgtt ttatacgtat cccaaaaccc attatttgta tagagaacac caggttttat
    22501 agatcgacta gtcgtatcat agtggatttt ttgcccacta ggatcaattt cataaaatgt
    22561 ttgtggaaat aaaaacaccc gatataaatt atgataaaac gtccgcaatt gttgttgatc
    22621 atggtgtgtg agctgtatac gatcaaaata ggactgccat tgttcttgac tgtgagcaag
    22681 gtaagtctct ttgctttggt tttgttctag ttccaggtta taagctgctt gttccgagct
    22741 gataaaagat gtcccaaatc ggatagtttg tttttttgtc tgttcaaaat acaacgggat
    22801 cgagtcgcta tttttgttgg aaacagctaa ctcatcaatt gttaaaggat attcaaatct
    22861 caatataaaa taaaaagaaa aatcaggatc ttctgaacca gcaaaatgtg tgatttttcc
    22921 ttccactgtg taaggatcta taacaaagag ctgatggcgt cccggaaagg ataaaagtaa
    22981 accgctgtca tttttgctgt aatcaataga taagattccg ccatacatgc taggtatcaa
    23041 tgtagaacag atgccataac gtaattgaca gatcgacaga tgagtcgggc aaaatatact
    23101 ttcttctggg cggtaagaac tttgagcatg gaaaagggtg ttctcctgta gttttccatt
    23161 gatcggcgta aacagcatat gactgaaatc acccatccaa ggacttggtt gatgggtaag
    23221 acggtagcct tgaaaaacgc ggtcatcagg atgaaaccac cagctgccgt tttgatccgt
    23281 agtttgcggt gcaaaataat tcatcccaaa aggaacgcca gtataaggct ggcagttccc
    23341 attagagaaa tttggctgat tcgctgttgc atgtctagta tctatttgac cgatattcat
    23401 tttatttctc ctttgttgat tgtcttttga tgaatgaaac tggaaattta ctatcggatg
    23461 gtcgatcttt tgtctctaac caagtgacca aagcatctcc cgcaagctta cctaactgtt
    23521 caaaatcttg tgcgatggtt gtcagtggcg ggtcaacaag agctgctgct tggatatcat
    23581 caaatccgat aattgaaaaa tcttcaggga tatgataacc atgctgtctc aagagattca
    23641 tggtctggat agcgacaaca tcgttttcac aaacaaaagc agttacttca tacttagtga
    23701 tataagaaag caattcatct aatgtcgcgc ttttgtcatc cagtgaagtg tggaaagtca
    23761 aaccagcttt gtccaatgct tcaaggtaac caaggtaacg ctgtcgaacg gattgtggat
    23821 gagtctgcgt accaaacaaa taagcgattt ttgaatgtcc ctgttcgatc aaataagagg
    23881 cagcaagcgc accgccttgg aaattatcgg aaagaacggt aggaaaaggt aactcataga
    23941 tttttttatc taatatgacg acagggaatt ctttcaatga aagctcaaaa agtaagtcaa
    24001 gatgttgttc tgtatcaaga gcataataaa tcaatccgtc aaattcattg attagctctt
    24061 ttgctgtttt attttgtaga aagtcaagcg tcgtcatcat gacatcaatc tgtttatctt
    24121 gcatcacagg gttcaatccc ttgctaaaat caccaacaga caaatcagcg acatatggaa
    24181 gaagaaacag aatccgtcgt gtttttgtag gagaagcaag aatctgttcc ggagaagcgg
    24241 atttaacgaa actgcctttc ccgcgaacgc gataaataag tcctaattgc tctaattcag
    24301 tcaatgcacg tttggaagtg attctgctta ctttataggt acgtgaaagt tctttttcag
    24361 ttggtagttg gctgtctaca ggtaattcac cagttaaaat agccgtttgc aaatcattaa
    24421 atattgtctg gtacaatggt tgagtcatat aaacacctct taatgatata tcatattcaa
    24481 ataataccat aatggatgaa aaagtaaata aaaagatatt ttttgaaagc gtagacataa
    24541 ttggctgtat gaagtataat ctttaaagat atatcaaata gaaagaaggt tttattagtg
    24601 gtttataaag atataccaaa atcggtgcag aaatttatgg aagatatcac acaaaaatgt
    24661 ggggatatcc atgcggactg ggcaaagaat tttacagcag catttgccaa tactctgttg
    24721 acaacagtca aaaagcaaaa tgacggaacc acctttttat tgacgggaga cattccagcg
    24781 atgtggttaa gagactctac tgcacaagtt cgtccttatt tagtgattgc aaaagaagat
    24841 gaggaattag ctgacatgat cagcgggtta gtcaaaaagc aattttttta tatcaatatc
    24901 gatccatatg cgaatgcctt caacgaagaa gctaacggtg ctggccacca aacagatcat
    24961 acaaaaatga acgattggat ctgggaaaga aaatatgaaa ttgattcttt atgctaccca
    25021 atacagttgg cctatcttct ttacaaaaat acaggaaaaa ctgatcaatt caatcaatct
    25081 tttattgaag gtatcaaaaa aatcttggac gtgttcaaaa cagagcaaga tcatgaacac
    25141 tctccttata catttgtacg agacacgaca aggaaagaag atacgctgat caatgacggg
    25201 cgaggtgcaa aaacagcttt tacaggaatg acatggtctg gtttccgtcc tagcgatgat
    25261 gcttgccgtt atggatatct ggtaccatcc aatatgtttg cggtggtcgt gttaggttat
    25321 gtggaagaaa tttttcaaac gatactcgat gataaagaaa ttgtacagca aacacagaaa
    25381 ttgaagaaag agatccaaga ggggattcag aatcacggac gaacggttaa tcaaaaaggg
    25441 caaacgattt atgcatatga agtagatgga atgggaaacg ccacagtcat ggatgacagt
    25501 aatgtgccca atctgatcgc cgcaccttac ttgggatatt gttctgaaca agaggaagat
    25561 tatctgcaaa caagaagaac attgttgagt aaagaaaatc catatttcta tgaaggaaag
    25621 tacgcaaaag gaattggatc ctcccatacg ccagaaaaat acgtttggcc gattgcatta
    25681 gctatggaag gaatgacaac gtcagacaaa tctgaaaaag agcggatatt gaatttactt
    25741 gtagcaactg acgcaggaac acaccttatg cacgaaggat tcgatgtgaa caatcctgaa
    25801 aattatacga gagaatggtt ttcttgggca aatatgatgt tttgcgaatt agtcatggat
    25861 tactttgata ttagagtaga aaagtaggag gtatcgtaaa tgaaaaagaa agtatatatc
    25921 atttctcata gccactggga tcgtgaatgg tacatggctt acgaacagca tcacatgcga
    25981 ctgattgaat tgatagacga tctactagag ctatttgaag ttgatccgtc cttcaacagt
    26041 tttcatttgg atgggcaaac gattatttta gatgactatc ttcaagtaag gccagaaaaa
    26101 agacaggcaa tccaacaagc aatcaatgaa ggaaaattga gaattggacc attttatatc
    26161 ttgcaagatg attttcttat cagctcggaa tcgaacgtga gaaatatgct gattggcatg
    26221 gaagaaagcc gtaaatgggg aacccctgtc atgttaggct attttcccga tacatttgga
    26281 aatatgggac agacgcctca attaatgaaa caagctggta tttcagcagc agcgtttggc
    26341 cgtggtgtaa aaccaattgg ctttgataat caagttttgg aagcagaaaa ctattcttcc
    26401 caatactcag agatgtggtg gaaaggccct gatcaaacag caatctttgg tctgctattt
    26461 gccaattggt atagcaacgg gaatgagatt ccagtagaaa aagaagcagc tcttgccttt
    26521 tggaagcaaa aattagcgga tgcagaacaa tatgcctcta cgaaccactt attgatgatg
    26581 aatggtgttg atcatcaacc ggtacaaaaa gatatatcaa aagcaatcca tctcgcaaat
    26641 gaactgtttc cagattacga gtttattcat tctaatttta ctgactatct ggaagcagtt
    26701 caaaaagatg tgccagaaga cttaggctca gtagaaggag aattgacaag tcaagaaacg
    26761 gatggctggt atactttagc caatacagca tctgctcggg tttacttaaa acaatggaat
    26821 accaaggtcc agcgacagct agaaaatatt acagaaccac ttgcgacgat ggcttatgaa
    26881 gttagtggaa actatcccca tgatcaatta gattatgctt ggaagacatt aatgcaaaat
    26941 catcctcacg acagtatttg cggttgttca gtcgattctg tccatcggga aatgatacct
    27001 cgttttgaaa aagcagatga agttggaaag tatcttgcac aagattcctt agaacagctg
    27061 accgcagcaa tcgatacgac cggattccca aaagacagct ttccatttgt cattgtaaat
    27121 actgcaggaa tggataaaac tggtgaagca gaaataacca tcgaattaga aagaaaaaga
    27181 ttcgctgaag gaatacctga acaactatat caagagttag aaaatttgcc taaacgtaag
    27241 tatcatgtag aaacaaaaag tggggccact ataccagcta ttttatctga agaaactgtc
    27301 caatttggtt atgatttgcc gaaagaccgt ttccgtgtgc catatatggc aagaatgatc
    27361 aaagtgactt taccattaga aaacatgcca gcattttctt gggaaacatt cgcgcttgta
    27421 gaaggggaag cgaaagcaga agaaaaggaa acaatgattc atcagagtgg acgaatcatc
    27481 gaaaacggcc ctctacattt gacgattgaa aaaaatggta ccattacaat ggaagatcgc
    27541 aaaaacaaac gaaagttgaa taatctgcat atttttgaag atatcggcga tataggaaat
    27601 gagtatatct ttaaacaacc tttctgtgat cagccaatcc tatccagtaa caaagaaaac
    27661 tctgaagtaa aagtattagt agatacaccg gaaatagcaa aaataagtat tctacaggaa
    27721 atggaaatcc ctgtttctgc agacgagcgt ttagaaaaag aacagcagat ggttgtggaa
    27781 tttcgttatc gaaaagcaga acgatccaaa gaaaaacgaa tccttcaaat aaaaacaatc
    27841 atgacaatca gaaaagatag taaaaaaatc gactttgaaa cgactctcga caatcagatg
    27901 aaagatcatc gcttacgtgt cttgtttcct acaaagttac atgtggaaca tcacgaagca
    27961 gatagtattt ttgaagtggt gaagcgccca aaccatgtgt ctaaaagttg ggaaaacccc
    28021 acgaatccgc aacatcagca agcatttgtc aatattcatg atgaagagta cggtgtaaca
    28081 gtcggaaact ttggtttgaa tgaatatgaa gtaacagaag acggacaaat cgcagtgacg
    28141 ctacttcgca gtgtgggaga actaggcgat tggggatatt ttccaacacc agaagcacag
    28201 tgtctaggtg agcatcggtt caattacagc attgaactac atggaccaga agaaaaattc
    28261 tctacttatt tgcatgcata cgcagcacag attccttttt ccactcaaca aatcaaacat
    28321 catgaaggaa cattgatttc aaaacaacaa tatttaacga taaaaagtga aacgtttgca
    28381 attacagctt tgaaacgcag caagtttagt gataaagtag tagtaagagg tttcaatatg
    28441 tctagtcatt tagaaaaatt ggaaataact aaagacaatg gaaaaactgt catattgaat
    28501 ctgcttgaag aaccaacgaa gcaagcggtt gtcccaataa tccaacctta tgagattcgg
    28561 acaatcggat tcgaggagga aaacgaatgt atggcggaat agaagcaggc ggaacaaaat
    28621 ttgtgtgtgc ggtttcagat ggtggagaaa ttttagaaaa attaagtatc cctaccacaa
    28681 caccagaaga aacattggat caggtatttg aattttttga tcgttatgaa ttgaagggaa
    28741 tcgggattgg ctcattcggc ccgattggta tcgacaaaac aaaagataat tacggatacg
    28801 ttttggcaac tcccaaaaaa gggtgggcac aattcgattt tcttggcaca atcaaggaac
    28861 gctacaatat tcctgctgct tggacaacgg atgtcaatgc tgctgcatat ggggaattga
    28921 agcaaggagc agcacaagga aaaaatagtt gtatttacct taccgtggga acaggaatcg
    28981 gtgcaggtgt cgtgatcaat gaagaaattt tttcagggat cgcacatcct gaaatgggac
    29041 atatttgggt gaaacgccat ccagatgacc attatgaagg tacttgtcct tatcataaag
    29101 attgtttaga aggattagca gcaggaccgt ctattgaggc acgcacaggg atcaaagggc
    29161 aagatctgcc gcaagatcat cctgtttggg ctattcaggc ttattatatt gctcaagcac
    29221 tgatcaacta tacgttgacg ttagcacctg aaaagattat tttaggcggt ggggtaatga
    29281 atcaggatca tctccttcaa aaaatccgtc agcaatttgt tgaattgatg ggtggttata
    29341 tggaaacacc tcaagtaagt gaatatatcg ttcgttgggg actgccgaat gaaagtggga
    29401 ttatcggtag tttgctgtta gcagaacacg aacatcaaaa gtaagcagac aaattacaca
    29461 aaaagaacag agtcttatca ttatctgctg ataagactct gttctttttt atttctattc
    29521 ccataatcca ggtaaagctt taccgactat tttttcttga gtaaatggat ggatcatgtg
    29581 cagttctaat gcatggagca tcagtcgttc agctgttcta ttttggtaga gagggtctcc
    29641 aacgatcgga tgtcccttat gagctaaatg aactctgatc tggtgtgtac gtccggtttc
    29701 caaaacacaa tagacttgtg tttgttcaga aagtactttg ttgacagtga catgtgtcaa
    29761 ggcgctctga ccatttttat catctacgac cctttttctc cgatcatggc gatctcgtcc
    29821 aattttattg gtgatggtca tatctttggc aatttctccc catacgatgg cttgatatct
    29881 ccgatagatc attttttgtt ccaacattct tccaagtatc ggcaagacaa atggattttt
    29941 tgcaaataaa acggcaccgc ttgtttcttt gtccaagcgg tggacaacgt aaggtcgttg
    30001 ctgtttagtg tcgagataag cagctaaatg attgagtaag gtactgtttt ctgtcggttc
    30061 atttggatga gtcttcattc ctgcaggctt atccactata atcaaatgct catcttcata
    30121 taaaacacgg atgtgcttcg catctcctaa ttgaacttct tgataattat aatctgtttc
    30181 ttcgaataac aatgtgatct ggtcattgcc ctttacttca tgatggaaca aagcaggttg
    30241 ttgattgatc caaacgtttt tacgtatacg caaaaagtgt cgaacctttc taggaactaa
    30301 ccattccttt tcaagcaagt cgcgaattgt tgtttcagga tggttcatag gtaaagtgat
    30361 cgtgattttc acaagtgtac tcctttcagt gcaattgcag actaattgta ccatttttaa
    30421 aacatattta agaaaaaact tacatttttc tgttcattct tatgttactc tacttgcact
    30481 aacgtaaaaa aacatattga actacaactt aagcaggaag aaaacactct ttcactatgg
    30541 taaaataaga cgattgaatc aaatagacag gagaagaagc aaatggattt tcaaagcatt
    30601 ttagaaaaga taaaagctgc tttgatcagt ttttggcgtt ggatcagacc atatctcgga
    30661 caattccatc gatggcgaaa gcgcatctgg aaaaaatatc atgtgaacaa attgattttg
    30721 ctgattggtt tgatctgtgt cttagtgaca agtatctacc tctttatttt agcaaaacaa
    30781 gcaaatgtcg aaacgttaaa atcaggatta agccaatcga cagtcattta tgataagaaa
    30841 aacgaagaag ctggcacact gtacgcccaa aaaggaacat atgtagaatt aaacgcgatt
    30901 tctccctata tccaagatgc agtaatctcg acagaagata ggaattttta cgagcatcac
    30961 gggtttgata tcaaagggat tgcccgtgca gcagtccgaa tggtcttgtc tggcggaaca
    31021 tcagggggcg gaggaagtac gatcactcag cagcttgcca aaaatgcgta tctgacatta
    31081 gaccaaacgt ttgatcgaaa agcgaaggaa ttatttttgg caatcgagat cgaaaagaag
    31141 tacagcaaag aagaaatctt gaccatgtat cttaacaatg cctattttgg caacggggta
    31201 tggggtgtcc aagacgcttc cagaaagtat tttggagtag atgcaacgaa tgttacgctg
    31261 tctgaggctg ctactttggc aggaatgctg aaaggtcctg gtatttataa tccgatcgat
    31321 cacccaaaaa atgcgaatga tcggaaaaac acagtactaa gtgtaatgga agaaaacgga
    31381 aaaatcacaa aagaacaaga acaagcagaa agccagacag atatcagcgc gtatcttaat
    31441 gacacatacg ataattcaga ttctggttat cgttatcctt attattttga tgcagtgatc
    31501 gatgaagcag ttaacgagta tggtcttgat gaagaagatg tcatgaataa aggctataaa
    31561 atctatacag ctttagatca ggattatcaa aagcaattag aagctactta tcaaaacagt
    31621 gcattattcc cagcaaatgc ttcagatggt gccatggtcc aatctgcttc agtagcttta
    31681 gatccgaata caggtggtgt acaagcacta gtaggacgaa gaggagatca tgtctttaga
    31741 ggattcagct ttgctacaca gatgcgtcgt tcaccaggat caacgatcaa accattaagc
    31801 gtatatacac cagcactgga agctggatac aagccttcca gtatcttgaa agacgaaccg
    31861 caaagttatt acgatgcgca taattttgac gggacatacc aaggagaagt accgatgtat
    31921 caagctgttg cacaaagttt gaatttgcct gctgtatggc ttttgcatga gatcggttta
    31981 caaaaaggat atgataaagc agaagaattc ggcctgcctt tagcaaaatc tgataagtat
    32041 tatggcttag cccttggtgg actggaaaaa ggagtatctc cattgatcat ggcgggtgct
    32101 tacagtgcat ttgccaatga tggtcagatg tatacgccgc atcttatcac gaaaatcgtt
    32161 gattcgactg gagcggtgat cgtcgataac acaaacaaaa aaccaaaaca ggtcatatca
    32221 aaggaaacag ccgatgaaat gaccagcatg cttcttggta cattttcaaa tggaacagcg
    32281 gctgctgcga atcctgcagg ttatacgata gcagggaaaa caggaaccac agaaactaat
    32341 ttcgatgcca caaaagcaaa tgaccaatgg atgatcggtt acacgccaga cgtagtgatt
    32401 tcgacatgga tgggttacga agaatccagc aagctgcact atttggaagg gacaagtgga
    32461 aatgttgtcg ggaaagtctt caaatcagcg gcagaaggca tccttccgta tacttcaaaa
    32521 acaggattta gtgtagcaga tgcctatgcc acaggaggac aagtggtagc tgcagatcaa
    32581 gttggaaaca caggaaattc taatggagag tctggaaatt ggcaagataa cttgaatcaa
    32641 tatggtgaac aagcaaaaga agggctgaaa aacttcggcg atatggtgaa agaaggcgtt
    32701 cagggaattg gtgaagcggc taaagacctc tggcggaaat atcaaggaga gtagcccctt
    32761 tgaaaacgaa taaccccatg ttacaataga gagtaactac taaaagaaaa gagggcataa
    32821 catgtcaaac atctacgata gcgcgaatca aatcgaacgt gagatccgtg aattaccaga
    32881 attcaaagcg ttgaaagaag cgtacgagaa agtacaagca aacgaagaag cacacaaact
    32941 gttcaaagac ttccaagcga tgcaaatcga attacaagaa aaacaaatga gtggacaaga
    33001 gttcagcgac gaagatgcaa tgaaagcaca agaaatggct gtgaaaatcc aatcagaaga
    33061 agtaatcaat gacttgatgc aaaaagagca aggcttcagt acaatcatca atgacttgaa
    33121 ccgtatcatc atgacaccag ttcgcgacct ttacagcgaa taaaactatt ttatgaaaca
    33181 acagagggtg tgaaagagac gttcggcctt gagcgatgac aaaattgctt cttattgccg
    33241 ataggctgtc tcttgaacac catttaccaa gaggtcgtga gcctaccgtt taactctgag
    33301 ttacaaggaa caatttcaaa aaacagcttc tcatgtttga tgactattgt gacttgtgac
    33361 cgaggagttg gcaggagaac accgtttatt cagaaatagg agctacgaca aaagtcttgt
    33421 catagctcct attttttgtc attcattttt caagcggtag aaatagcctt tttgagctaa
    33481 cagtttttcg taagtaccta tttcaacgat ttttccttca tcgatcacaa cgattttatc
    33541 tgcctctttg atcgttgaaa gccgatgagc cacaataaaa gtagtgggtg tttctgtcag
    33601 gtaattcagg gcttgctgga tttttttctc tgactggtta tctaaagctg aggttgcttc
    33661 atctagcaaa atgatttttg gctgacgtat caacgctcgt gcaatggaga tacattgctt
    33721 ttggccacca gacagcttgt tgccatgttc gcctactaag gtatccagac catcaggaag
    33781 ctggtcaatc agtgaagaaa gttgcgcagc ttcgatgact tcatctaaac gttcttttga
    33841 tacgtttttc aagccataag tgatgttctc tttgatcgaa gcggagaaca acaaagtggt
    33901 ttgaggaaca acggctaaat aatttcgcac acttcgcata tccagttcat ccaaaggctg
    33961 atcatctaga aaaatttttc ctgaagtcgg ctggataaat ccaatcaata agttaaggag
    34021 tgtagatttt ccagagccag atggcccaac aaaagcaatt gtttctccag ctttcacctc
    34081 tagagaaaaa tgatcgataa tcggtttgag tgagtccgga taaagaaaag aaacatcctc
    34141 aaaacgaaaa tttccatcca aatgttggat ttttttctta ccgtgataag tttctacatc
    34201 gccagcaatc aacagttcgc caatagaagt gatagattcc atgccttttg aaatcgtagg
    34261 aatcagcgtg atcattccag aaacggaatt gacgatcgtc gtgaaatacg tctgatacat
    34321 cacgacatcg ccgggttgga tgcgcccttg taaagctaat atcccagtga aactcaaaca
    34381 aatcagctga aatatctgaa aactagccca gctgactgat ccgaaaacag attgcaggat
    34441 atctaagtgg aatccttttt catagatttg agtaaaatgc ttatttaagc gagttgtttc
    34501 gtgtttttct aacccatgag ctcgagcaat cggtgctaat tcgatcactt cgatcacttt
    34561 tgcagaagtc tcttccattt cttgcctgaa gtctgagttg gactgtctga tccttttacg
    34621 gaaaaagaga gtaacgaatg cggcgagagg aacagtcaat aagaaaaaca tgaaaacaac
    34681 aggactttta taaagcgtga tacctaaagc tacgaataaa ttgacagcga tatttaatcc
    34741 gctgacaaat acttgagagg acaacgtttg gatagcttct acatcacgga tgatctttga
    34801 ctggattcgt ccagattgca attctttttg ataaggaata gatagttctt ggattttctt
    34861 gataagggtc gctcttagtc cagcttctac ttgacggatc gaagtgctat ggtattttac
    34921 atgaaaataa ttcgtcaata tattttgacc aatcagacaa gccatcaaaa aagcgttgaa
    34981 ataaatggcc tgcaactgag aagaatcttt cgatgaagct gcattgatta cattggcaat
    35041 agcgatcggc attaaccaag atggaagatg tttgataata aaaaagagtc ctgacatggc
    35101 aaaacgccaa taatttccct gataaagatg cagcaatgtc tgaaaagcat ttgcatgata
    35161 tttttgataa cttttctgta ttcgttcctc cataatcttc tccttaacaa tcttggtaaa
    35221 taaactgcaa gtgttctatt gcttcaggaa cataaatctc aggagtagct tcaagagtgg
    35281 cgtacattag aggacgctca tatttgatat aatgcagtac cgggcggaaa tctaaacagc
    35341 ccttgccaac aggtaccatg cggatctttc cattttccat gatatagtct ttgagatgaa
    35401 gcgctgtaat ataaccatgt aaattgtcca aggcacctcg gatcactttt tcctgatcag
    35461 catgattttc tttatgcatc aaattcgcac aatctaaaat gatttttaaa tttggtgaag
    35521 ctacttcatc gattaaacgt ttagctaact gatagttata gattggatga ttgatccctg
    35581 cttcaacagc aacagttacg ccaaaatttt ctgcatgagc gactaattca atgatagaat
    35641 ttctggcgat ctgataagct tcttcagtaa aattttcttt tgtgtaaccg acagagacac
    35701 taccggtttc cgtagcaatc atcttggctt gataggcaga gcacagagca atttgctgtt
    35761 tgaaagttgc caatgcttgt gcacgaactt cttgattttt gctggatagg ttgatatagc
    35821 atcctaaaat ggaaagttcg atattgtttt tttggaagta ttcaccaaaa taggcagcac
    35881 tcccaagtgt catgtcagtt aatttaagtt gttcaggaaa tgctttttgt ggtgcaaatt
    35941 ggatatggga caagtgaagc tgtgatactt tttgaacgat ttcttcacga tttcgaacat
    36001 ctaaatcgtg ggcacggatg cctaaattta atggaaacat ctcaaactcc tttttgtaaa
    36061 taattagcaa gaggcagacg ggattctttt atcaattcaa caattattcg ggcaacttta
    36121 tcagcgcctt ctggactaaa gtgggtattg tcttttagtc cttctgggta atttttacaa
    36181 gtatcaggca taagatgcag gtagaatttt tttgtctctt cttttgagaa ggtctggaac
    36241 aattcttgtg atcttgagaa cacatctaaa agaggaatat ggtgtttctc agcaaatgca
    36301 cgcatcgctt ttggatagtc tccaagtata tctggattca atgttccgtt ttctaagtaa
    36361 tctcgtcgcg tcacagatgt cagtaagacc atttggacgt tcgctttttt tgcgcattca
    36421 aaaaatatct gcagattttt taaatagcta ccgaagggaa gcgtacctct ttcttcttgg
    36481 attttctggt cgttatgtcc aaattgtaca aataagaaat cattcggctg aaactgtgaa
    36541 gctagacggg ctaaccttcc ttcctggata aatgatttgg tgctgcgtcc attctttgct
    36601 tggttattga tctggatctc gggtgaaaaa taagcagaga ttttttctcc ccaacctgtc
    36661 tcgggacgct tgttttcatc tttttctgcc gcagtagaat caccggcaat atggatagta
    36721 ggcatgttat ctcttctttc ataaaggttt ataggcaatg aagcagaaca gcaagagaag
    36781 aagacggagc aa
//
LOCUS       DAZKKW010000019        35456 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.019, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000019 DAZKKW010000000
VERSION     DAZKKW010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35456
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.019"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            83..1849
                     /locus_tag="ACG8PO_000946"
     CDS             83..1849
                     /locus_tag="ACG8PO_000946"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HFQ0206290.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            1951..3210
                     /locus_tag="ACG8PO_000947"
     CDS             1951..3210
                     /locus_tag="ACG8PO_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFQ0206291.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            3357..4658
                     /locus_tag="ACG8PO_000948"
     CDS             3357..4658
                     /locus_tag="ACG8PO_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0206292.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            4661..5512
                     /locus_tag="ACG8PO_000949"
     CDS             4661..5512
                     /locus_tag="ACG8PO_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HFQ0206293.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            5525..6340
                     /locus_tag="ACG8PO_000950"
     CDS             5525..6340
                     /locus_tag="ACG8PO_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HFQ0206294.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            6491..7657
                     /locus_tag="ACG8PO_000951"
     CDS             6491..7657
                     /locus_tag="ACG8PO_000951"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFQ0206295.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            7887..8534
                     /locus_tag="ACG8PO_000952"
     CDS             7887..8534
                     /locus_tag="ACG8PO_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFQ0206296.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            8618..9562
                     /locus_tag="ACG8PO_000953"
     CDS             8618..9562
                     /locus_tag="ACG8PO_000953"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HFQ0206297.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            9697..11148
                     /locus_tag="ACG8PO_000954"
     CDS             9697..11148
                     /locus_tag="ACG8PO_000954"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HFQ0206298.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            11169..12005
                     /locus_tag="ACG8PO_000955"
     CDS             11169..12005
                     /locus_tag="ACG8PO_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFQ0206299.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            12005..12664
                     /locus_tag="ACG8PO_000956"
     CDS             12005..12664
                     /locus_tag="ACG8PO_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFQ0206300.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSMHQS
                     I"
     gene            12790..13479
                     /locus_tag="ACG8PO_000957"
     CDS             12790..13479
                     /locus_tag="ACG8PO_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0206301.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PTDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            13485..14441
                     /locus_tag="ACG8PO_000958"
     CDS             13485..14441
                     /locus_tag="ACG8PO_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HFQ0206302.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHPIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            14485..14997
                     /locus_tag="ACG8PO_000959"
     CDS             14485..14997
                     /locus_tag="ACG8PO_000959"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0206303.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSSVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(15253..15801)
                     /locus_tag="ACG8PO_000960"
     CDS             complement(15253..15801)
                     /locus_tag="ACG8PO_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFQ0206304.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(15890..17071)
                     /locus_tag="ACG8PO_000961"
     CDS             complement(15890..17071)
                     /locus_tag="ACG8PO_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HFQ0206305.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(17307..18815)
                     /locus_tag="ACG8PO_000962"
     CDS             complement(17307..18815)
                     /locus_tag="ACG8PO_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HFQ0206306.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATSLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVEAFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            18974..19165
                     /locus_tag="ACG8PO_000963"
     CDS             18974..19165
                     /locus_tag="ACG8PO_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0206307.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            19368..20285
                     /locus_tag="ACG8PO_000964"
     CDS             19368..20285
                     /locus_tag="ACG8PO_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coiled-coil domain-containing protein"
                     /protein_id="HFQ0206308.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            20278..21351
                     /locus_tag="ACG8PO_000965"
     CDS             20278..21351
                     /locus_tag="ACG8PO_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0206309.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            21499..22017
                     /locus_tag="ACG8PO_000966"
     CDS             21499..22017
                     /locus_tag="ACG8PO_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0206310.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            21998..22390
                     /locus_tag="ACG8PO_000967"
     CDS             21998..22390
                     /locus_tag="ACG8PO_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HFQ0206311.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVFFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            22466..23401
                     /locus_tag="ACG8PO_000968"
     CDS             22466..23401
                     /locus_tag="ACG8PO_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFQ0206312.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            23534..24313
                     /locus_tag="ACG8PO_000969"
     CDS             23534..24313
                     /locus_tag="ACG8PO_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HFQ0206313.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            24306..24623
                     /locus_tag="ACG8PO_000970"
     CDS             24306..24623
                     /locus_tag="ACG8PO_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HFQ0206314.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            24867..26477
                     /locus_tag="ACG8PO_000971"
     CDS             24867..26477
                     /locus_tag="ACG8PO_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFQ0206315.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            26634..27482
                     /locus_tag="ACG8PO_000972"
     CDS             26634..27482
                     /locus_tag="ACG8PO_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFQ0206316.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
     gene            27713..28192
                     /locus_tag="ACG8PO_000973"
     CDS             27713..28192
                     /locus_tag="ACG8PO_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6530 family protein"
                     /protein_id="HFQ0206317.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNTDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            28225..30453
                     /locus_tag="ACG8PO_000974"
     CDS             28225..30453
                     /locus_tag="ACG8PO_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0206318.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKGRTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            30582..32225
                     /locus_tag="ACG8PO_000975"
     CDS             30582..32225
                     /locus_tag="ACG8PO_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFQ0206319.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKLALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(32298..32843)
                     /locus_tag="ACG8PO_000976"
     CDS             complement(32298..32843)
                     /locus_tag="ACG8PO_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HFQ0206320.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(32866..33351)
                     /locus_tag="ACG8PO_000977"
     CDS             complement(32866..33351)
                     /locus_tag="ACG8PO_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFQ0206321.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(33341..34153)
                     /locus_tag="ACG8PO_000978"
     CDS             complement(33341..34153)
                     /locus_tag="ACG8PO_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HFQ0206322.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(34292..>35456)
                     /locus_tag="ACG8PO_000979"
     CDS             complement(34292..>35456)
                     /locus_tag="ACG8PO_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HFQ0206323.1"
                     /translation="RNFEIFFRILKLTSTTGIPILYLMEQPALSVSQSFLDKNEISYM
                     THTSGTTGIPKLICHSAHSMGWRTKWQKTVFTKISEKKLIAFHISPVHSRFNIGISSL
                     MSMGFPMMPLANAQSSKVVHMLEAHRPIALETHPNNFVQWRFTAKEHPEAFSGIRYYH
                     STFDTINNQTMKTFLRTSMAQDAVFLQVYGQSECGPMILKAHTLESLKTSDARDMGVG
                     LEDMTSARITDSVGNVLPENTDGHIQLLSKGRALTYYKEDARFQENVYGDWWDSGDYG
                     FMDKHGHLFLKDRQVDLIETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSPQPIIALA
                     PDKEMDWNRWWEQVHELPRLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQKS"
BASE COUNT        11584 a         6390 c         7095 g        10387 t
ORIGIN      
        1 gataacattt ttgaaagcgt tgtcttttgc ttctctatca cttatagtga tgacgtcata
       61 ttaaaacaca aggaggaaat caatggatac agctgcaatt tatcatcgac cggaaagtga
      121 atatgcattt ctatatacga atacaagatt tcgtattcgg cttcgtacta ggaaaaatga
      181 tatcaaaaaa gtatatgtgc tatgtggaga tccatatacc ataacaactg aaaaatggta
      241 tcaaaaacaa cgaccaatga aaaaatgtct aagtaccaat gttcatgatt attgggaaat
      301 tgaagtgacg agcgaaacaa gtcgcttaca gtatgcgttc catgttgttg gggaagataa
      361 cacggactgt ttttacggtg atcaaggaat ttttccttat cgagaaaatg tgattactga
      421 accaaatttt tattttcgaa taccttattt tcatcaaatc gatcgattca ctattccaga
      481 atgggtaaaa gaaacagtat ggtatcaaat ttttcccgaa agatttgcaa acggggacaa
      541 agcaaatgac cccgaacaca ctttaccttg gggaagtaaa gatccagacc gagaagactt
      601 tttcggggga gatttgcaag gtgtgatgga tcatttggat tacttgacgg accttggcat
      661 caatggcatc tatttttgtc ccatatttaa agccacatcc aatcataaat acgatacgat
      721 tgattattat gaggtggatc ccgcttttgg cgataaaaaa cttttaaaaa acttgattga
      781 tgaagcacat aaaagaggga tacgtatcat gttcgatgct gtattcaatc atatgggtgt
      841 ccattcacca cagtggcaag acgttctcaa aaacggagaa aaatcgatct ataaagactg
      901 gttccacatc cgttttttcc cagtagattc ttatcagatg actgacttac cagaaactgc
      961 tgaaaatatc ccatatgata cttttgcttt tactccattc atgccgaaac taaatacagc
     1021 aaatccagaa gtgcaaaaat atttgctgga tatcgcaact tattggataa aagaattcga
     1081 tattgatgga tggcgtcttg atgttgctaa tgaagtcgat catcattttt ggaaaaaatt
     1141 cagagaagca gttacagaaa tcaaaccaga catctatatc cttggagaga tttggcattc
     1201 ctctcaagct tggctgcaag gggatgaatt ccatgcagtg atgaattatg catttacaga
     1261 ttcgattaaa gattattttg caaaaaagaa aatcacagct tcacaaatgg tcagtggaat
     1321 gaatcatcag caaatgcttt acagagatca ggtgaacgaa ggaacattca acttattaga
     1381 ttctcatgat actgccagga tcttgaccct ttgccaaggt aacaaagaat tgatgaagtc
     1441 tgtgatggcc tttatgtttc ttcaaaaagg gtctccttgt atctattacg ggacagaaat
     1501 agggatgact ggtgaagatg acccggattg ccgcaaatgt atgatatggg agaaggagga
     1561 acaagatcta gagctattcg gttttataaa agaacttgtt tctctaagga aacagttgtc
     1621 taagattatt tctgaaggtt ctactcagtg gctaatagta aatgatcgtg aagataagct
     1681 ctatttcaca cggaaactag agggacagat catctatgtt tactttaatc agtcaaaaga
     1741 accttgggta gtagaacagg aaaatgaggt gattcttagt caaaattgtc agttgttaga
     1801 agacgggaaa gcagagatac gacaatacgg atttttgatc atgtgttgaa aactgttacc
     1861 ggtaacaaca agaaagcgtt tgcaaatgaa gatttgtgcc cattataata agggtgtaac
     1921 agcacgttga atacaaagga ggaaatgaag atgaaaaaaa gaagtgtgaa acagttgggt
     1981 ttagggttaa tctctttagg aatcttaggc ggattagcgg cttgtggcaa tggtaactct
     2041 tcagcagaag gggaagccga tgaaggaaaa acgttaacag tttccgttga ttctggatat
     2101 aaagattata taaacgaaat aaaaaacact ttcgaaaaag aaaacgatgt gaaaatcaag
     2161 ctagtagaaa aggacatgtt cgatcagttg gaatcattag cgttagatgg gcctgctgga
     2221 aagggacctg acgttatgat ggcagcatat gaccggatcg gtgcgttagg acagcaagga
     2281 catctggcgg aagtaaaact gggaaatgaa gcagcttacg atgaaacaga caaagcccaa
     2341 gtgacttatg atggaaaaat ctacggagaa cctgcagtaa tcgagaccct tgttttgtac
     2401 tataacaaag atttagtcga tacagcacca tcaacgttta aagatttgga gaatctatcc
     2461 aaagacagtc gttttgcttt tgagtcagaa gcaggaaaaa atacaggatt tttagctaaa
     2521 tggactgact tttattattc ctatggttta attgcaggtt acggtggcta tgtatttggt
     2581 gatgatggca caaatccttc agatatcggt ttgaataatg caggtgctgt agaaggaatc
     2641 tcttatgcaa cagattggtt caaaaatgtt tggccaaagg gaatgcaaga cataaaaagt
     2701 gcaggagact tcgccagcca gcaattcatg tccaataaaa ctgcagcaat cattgacggt
     2761 ccatggcaag cgcaaacata caaagaaaac aatatcaatt atggtgtagc caaaattcct
     2821 actctaaata atggacagcc ttatcaaccg tttggcggag gaaaaggatg ggtagtcagc
     2881 aattatgcta aaaacaaaga cctctctcaa aaatggcttg attatgtaac gaatcaggaa
     2941 aatcaagaaa aattctttga aatggtaaat gaaatccctg caaaccaaca agctagggaa
     3001 acagcaaagg ctaaaaacga tgaattaaca acagctgtga ttgaacaata tgaaacagct
     3061 caagcgatgc caaacatccc agaaatgggc gaagtttgga caggtgcaga aaacttgatg
     3121 ttcgatgcag catcaggttc aaaaacacca aaagaatccg cagatgaagc agtaaaaaca
     3181 atcagtgaag ccatagaaca aaaatactga ttaaaatgat aaccgtccat ttttataaaa
     3241 aatggaacag gtgcttcaga taaaagtggc aagagcgaga tggaaaatta tcttgctctt
     3301 gttgccatta tcttaaaagc agtttgcggt ttagcaagtt tagagggggc attcagatgc
     3361 agcaagataa aacaagcaat atcaacgaaa aaaaaatcac gctttgttca cttattccag
     3421 ggatggggca gtttgtaaat ggccaagctt ttaaaggtat cgtttttttc gccatattcg
     3481 tcctattcat tggtcagatg cttctgggcg gatttcatgc attggtagga ttggttactt
     3541 tgggaagtat accaatggaa gatcattcat tatttatttt gattgaaggc actttgcaat
     3601 tcatcattac aatcattttc ttgatttttt acggtttgaa tcttttagac gcttatcgag
     3661 tagcgaagat gcgtcaagag ggcaaaaaaa tcaatcggac gatgaaggaa gtcatacata
     3721 atataggtga tgaaggtttt ccttatctat tgacacttcc cgcttatctt ctcatgattt
     3781 ttacgatcat tttcccagtc ctagttacat tgttcactgc atttactaat tatgacttca
     3841 atcatattcc accagccagt ttaattgatt gggtaggggc taaaaacttt tttagtatct
     3901 tctttttaag ctcatatcgt aatacttttt ttgcagtctt ttcgtggacc gtgatttgga
     3961 ctttatgtgc aacaactttg caaatcaccc ttggtattct cacagcggtt gttgctcacc
     4021 agaaatttat taaattcaaa cgtattttcg gtatcatttt cttattacca tgggcggtcc
     4081 cagcttttat tactatcatg agtttttcga atatattcaa cgatagcgta ggtgccatca
     4141 atactcaagt gattcctttc ataaaccagc ttccttttgt agatatcagt gcaatctctt
     4201 ggaaaacgga cccgaactgg acgaaagtag caattatctt agtgcagggc tggttaggat
     4261 ttccctatat ctatgtgatg gttacaggaa tcttacaatc gatttcagaa gacttatatg
     4321 aagcagcaaa aatcgacgga gcaaatgcat ggcagcgctt ttcaaacatc acgcttcctg
     4381 ctgtcttcct agtggcagcg ccaacattta ttacacagta tacggggaat ttcaataatt
     4441 tctccatgat ctatctgttt aacaatggcg gtcccggaag cgtaggcgga ggagctggtt
     4501 ctactgatat tcttatttcc tggatctata aacttacgac tggaacttct ccgcagtatt
     4561 ctatggcttc agcaatcacg ttgattatct ccgtcatcgt tatctgtgtg tcattgctgg
     4621 tatttaaaaa gacaaatgcg ttcaatatgg gggattaaca atgaaaaaat ttcattcgag
     4681 tgctcgattc caaaaacgac tttctcactt ttttacttac ttttttcttt taatactgtc
     4741 agtcattatc atctatccgc ttttgatcac agcaagtact gccttcaagt caggaaatat
     4801 tgcagctttt agtttagatc tttcaagtga ctggacttta ggaaacttta ctcggctatt
     4861 cagtgaaaca ctttacggga cttggtataa aaatacattg atcatttctg tcctaacaat
     4921 gatcgtacaa gtgactgtag taactttggc gggctatact tatagtcgct atcgttttat
     4981 cggccgcaaa tcaagtttga tgttcttttt agtgattcag atggtaccga ctatggcagc
     5041 attgactgcc ttttatgtca tggctttctt attagaagcg ctcgataagt actggttctt
     5101 gacaatgatc tatataggcg gtggaattcc aatgaatact tggctgatga aaggatactt
     5161 tgacactgta ccaatagatt tagatgaatc tgctaaactt gatggggcag ggcatttcag
     5221 gatttttttc cagattgtat taccgcttgt caaaccgatg ctcgccgttc aagcattatg
     5281 ggcttttatg ggaccttttg gggatttcat gttagctaag ttcctgcttc gaacacctga
     5341 aaatcagact gttgctgtcg gcttacaaac atttatttct gatgtgacaa accaaagagt
     5401 ctctcttttt gctgctgggg caattttgat tgcactaccg atttgtctat tattcttctt
     5461 tttacaaaag aactttgtta ccggcctgac atctggcgga acaaaaggat aggagcagag
     5521 tatcatgcaa acatttccgt taaactattt ttccgcatta cgtacgccaa ctcaagtgtt
     5581 tgcgggacgc aaactattat catggcctaa atttttcttg atatttgtat ttttagtttc
     5641 cttgatggtc atgcctgtga ctctttttta tgccaatcag atccaagcga ttccattaga
     5701 acaatttcta tccgttcatt cgctgatcga tgaacaagga acccaaaaat tttctgaact
     5761 tgaattatct gaaactgggt tacaagcatc gcaacaaacg atagctgtga cccctgaaat
     5821 tttggtaggt gtgtctttat ctgagaaaca acaatccgat catggaactt ttattgattt
     5881 tgagaaggaa caatgggtga tccaacaaaa ggataaatct ggtatacgcc gctatacgat
     5941 gaattatagc ccatctttcc aaccagattc tgtacgtacg cccgaagatt tccaacgatt
     6001 tttagaaaga gaattttatg ccagtaatcg tccaacaatc attttatctt atagtttaag
     6061 tcttggtctt gtcctatttg tcatgacgag tctcatttta ttcggggctt cctttttctt
     6121 gtggatgaca agaaaaagcc aactttcatc cattcataca ttcaaagaga gtgcaaattt
     6181 gatgctgaat gttatgggaa taggttccat ggcagcagca gttgtagggt ttatccactt
     6241 tgactttatc ttgatgctaa gtgtccaaac aacgataact gtcttgctgc ttttatgggt
     6301 ttttgtcaaa acaagattca aagataagcg tgtcgaatga caagtttaag cgatttatgc
     6361 gacaatgaat gtgaaaaaaa tgaaaaataa agtagttttt gaaaaatttc tgaaaaaatt
     6421 aaacttctta gttgattctt ttctttttta gtattaacgt aatagggtag tttttttaag
     6481 gaggaaaatc atgttagaga aaactttata caatcaatta ttttcacgtt ctttctcgct
     6541 ccctgtcgaa gtaaattatt gggacggtac aacgaaacaa tacggagata cggacactcc
     6601 tccccaaatc aaaatcaata tccatgaaga aatcccaatg aaagagatta caagtaacgc
     6661 ttctttggca ctaggagaag cctatatgga tcatcgaatc gagatcgagg gaagtatcca
     6721 agcattgatc aacgatgctt atcagcaagc aaacagcttt ttacgaggaa aagattatct
     6781 aaaatggctg ccaaagaaag aaaaacatac gaaacaaaaa aacaaagaag atatccatgc
     6841 gcattatgac ttaggtaacg acttttataa actttggcta gatccaactt tgacttattc
     6901 ttgtgcttat ttcaatacac ctgaagatac gttggaacaa gcacaaatca ataaagtcca
     6961 tcatatctta gacaaactct ttatcaaaga aggcgaaacg ttgcttgata tcggttgtgg
     7021 ctggggaaca ttgatgttca ctgctaccaa agagtataat gtcaaggcaa ctgggatcac
     7081 actaagtgaa gaacaatacg atttgattac caaaagaata aaagaagaac atttagaaga
     7141 taaatgccgt gtattattaa tggattaccg cgaactaaaa ggcgaaacgt ttgatcatat
     7201 cacaagtgta ggtatgttcg aacatgtagg ttctgaaaat cttgaaggtt atttcaaagt
     7261 ggtaaaagat ttattgaaac caaaaggaac agctttgatc catggaatca gccgacaaca
     7321 aggcggtgcg acaaatgctt ggatcaataa atatattttc cctggcggtt atgtccctgg
     7381 tgttactgaa ttagttgata atattacaaa aaacgatttg caactgattg atttggaaag
     7441 cttacgtcgt gactaccaat tgacattaga acattggaca aagaattttc atcaagtcca
     7501 agatcaagtc acgaaagaaa tgggtgattc cttctatcga atgtgggatc tatatttaca
     7561 agcttgtgca gcttcatttg aatcaagtaa tatcgatgtg attcaatatt tgcttgttca
     7621 acctggaaac aatgacatcc caatgcatcg tgcataattg aggcaaaagt actgtttaaa
     7681 gactatttaa aaagaagcta agttttatga tgctccagaa gtgaactttt ttgtctaact
     7741 tctaggattg tctctaaagc ttagtttctt tttctttata ttttcataat aaaagtttgg
     7801 ttctttaatt tttaaacatg acagaaaatt ttttcttcgt tatacttgta atagaatact
     7861 attttataac aaaggggatc gaatcaatga aaaaaactgt tatttatact agtttatttg
     7921 cagctttgtt tttagctgct tgtcaatcaa ataccaatca aggcacatct gggaatacgt
     7981 cttccagtag cgaacagcgc tcttcacagg tatcggactc ctcaacaaaa gcaagcacat
     8041 cttcgtctag ccagacaaaa agttctagtg aagcaagtac tcagtcagat atcaaagtgt
     8101 ctgcagctcg tgcgatcgaa ttgttccaag ataaataccc agatactgca attactagtt
     8161 tggaattaga tagtgattgg ggatcttact tctacaagat agaaggtgtc gatgatcaaa
     8221 aagaatacga agcaagaatt gatgctcaaa atgaaaaaat ggagacagag aatccagaaa
     8281 ctctagatcg agatgaacaa aatggtcaaa agaaaaaaga agatggtctg gatgttgata
     8341 aattgatcac gattgaggaa gcagcaaaaa tcgccgtcga caaagtagga agcggtacgg
     8401 caacagattg ggatttggat aaagatttag gtactactta ttgggaagtc aaagtaaaag
     8461 acggcaatca aagtacatca gtcaagatca atagccaaac aggcgaagta ctgtcaactg
     8521 aacaagatga ttaagaattt cttcggaaaa caagagttga tttttctttt agtcatgata
     8581 cactagacaa aataacttgt ttgaaggagc gatttacttg agtttagaaa cgacctttgc
     8641 caatcatatc tttgccaatc ctttgatgaa tgcatctggt gtccactgca tgactaccca
     8701 ggaattagat gaactggctc attcagaagc aggagcattt atcactaaga gctgtacgat
     8761 caatgaacga aaaggaaatc ccgaaccacg ctattttgat gttcctttag ggagtatcaa
     8821 ttctatggga cttccaaact taggtttttc ttattatcta gagtatgctc ttgcgtatga
     8881 aaaagtgcag gaaaaccaaa atcaaccgtt atttttttcc attgccggga tgagtgtcca
     8941 ggaaaacttg gaaatgcttg agaaaataga aaaaagtggg tttaatggga tcactgaatt
     9001 aaatttatct tgtcccaatg tgcctggaaa accacaacta gcttacgatt ttgaggccac
     9061 ttatgaaaca ctgaaagaag tattcagcat cttttctaag ccgctaggaa tcaaattgcc
     9121 tccttatttt gattttgctc attttgatca gatggctgat atcttgaatc agtttccatt
     9181 gacctatgtc aatgctatca acagtgtggg caatggtttg tatatcgata cagaacaaga
     9241 agcagtagtc atcaaaccaa aagaagggtt cggcggcatt ggcggagagt atatcaagcc
     9301 gactgctttg gcgaatgttc gcgcttttta tacacgtttg aaaccagaaa tccaaatcat
     9361 tggtacaggt gggattcgga caggacaaga tgcatttgaa catctgctat gtggcgcaag
     9421 catgttacag attggtaccg agcttcataa agaagggccg gaaatttttt ctagaatcat
     9481 aaaagaatta acgcagatta tgtcagaaaa aggatatacg tcaattgacg aatttaaagg
     9541 caaattacgc accatttcat agcaaagaga cgaaggtcat gattgatctt cgtctttttt
     9601 tataaaaaac actcgtctgt tcattcctgt gcaagcgtac tcattgtatg ttagacgaac
     9661 ttgtgggaaa ataacagagg caaaaaggag gataatatga atgatgcaac agaattagca
     9721 aaagagctta aagaaaaaga atttagtttt caagaactag ttacggatat acagaaaaaa
     9781 ataaagaagc aaaatcatga attgaatgcg tttgtcgcgc ttgaaccatt cgagagtaat
     9841 tcccacgagc tattgattcc ccagtcagaa aaagggccat tggctggtat cccatttcct
     9901 ttaaaaatgc tcggtcaaga aaaaaaggga tggctagcta cttcaggatc tcgtttattt
     9961 gaaacacacc gagcttctca tacatctaat tatgtgcggc aagcagaagc gataggtcta
    10021 gttccttttg gacaaaccaa tgcgccagag tttggtttca aaaatattac agacccagtc
    10081 atctatggcc cagcaagaaa cccttggaac cttgaatgta caccgggcgg atcaagtgga
    10141 ggagctgctg ctgcagtttc atcaggaatc gttcctttag ctggtgctag tgatggcggt
    10201 ggttctatta gaatacccgc ttcgttttgc ggattgattg ggctgaaacc ttcgcgaggg
    10261 acgatgccag ttggtccata tgcttggaga ggatggcaag gagctgctat tgatttcggc
    10321 ttgacagttt ctatgagaga cacggaggct ttattttatg ggatgagaag tatccattcc
    10381 ggtgcgccat atcaagtatc gccagttgag tggcaaacac atccaagaaa aaaacggttg
    10441 aagatagctt tttgtgttga ttcaccgatc cactcacaga ttagtgaaga ggcagtactt
    10501 gctgtaaaaa aagctgtaga tttccttgga aaagaagggc atgatttgac agagattcct
    10561 tacccactag atggtcgtaa actgatcacg tcttattatc agatgaatgg cgcagaaaca
    10621 gcagcaatga tccgctcgat tgaaacaggc atccagcgag ctgtcagaaa agaagaactt
    10681 gaacccattt cttggacttt gttccaatat ggtcagaagc tctctgccgc tacttacatc
    10741 gattctctgc atgtatggga tgaagcagca attgtcatgg aaaacctaca tcaagaatac
    10801 gatctattcc tttcaccaac gactgcattt accgccccta aaatcagcga agatttgcaa
    10861 agtgatgcga taagagcaca gatggaacac atcagtgatt gttctgaagt agaaggttta
    10921 gacgttgttt cttccatgtt tgaggagagc ttgaagatca caccttatac acagttggct
    10981 aatctgactg gacaaccagc aatcagcttg cctactcatt tgacaaaaga aaacatgcct
    11041 ctaggaatcc aatttatggc tgccagagga agagaagatt tgctatttca aatcggctat
    11101 ctatttgaag caaatcatca gttccattta ccaaatccat ggaaatagca gaaacaagga
    11161 gaagaagaat gaagaaaaaa agattgtttg ctctaataac tagtgccttt tttattttaa
    11221 gcgcttgcgg tacagctgcc gctaatcagg aagataacag ctggagtaat gtagaaaaaa
    11281 agaaagaatt agtagtagcg acttctggca cactgtttcc cgcatcttat tataatgatc
    11341 aaaatgaatt agttggttat gatgtagatt tggcaaaaga ggtggctaaa cggttaaatg
    11401 tcaaaatctc ctttaaagaa tacaacgtgg atggacaaat cacttctgtt tcaaaaagag
    11461 aatcagattt tgctgcgaac gattttggta tcacaaaaga aagagaaaaa aaattctccc
    11521 tttcagagac tttcaaatat tcatttgaca gtatgatcgt acgaaaaaaa gatgattcag
    11581 gaatccactc acttgaagat cttaaaggga aaaaagcagc tggcgaaccc aatactagct
    11641 atatgcgttt agcggaaaaa cacggagcta aattagtcac atatgacaat gccacgaatg
    11701 atcagtattt aagcgatgta gcaaatggtc gaacagatgt tattctaaat gattattatc
    11761 tgcagaaaat ggccgtatct gctttaccag atatccctgt aaaaatactg gagaacgttt
    11821 atttcaatga aaacgaaagt ggttttttat tgaaagaagg aaatgatgct ctgaaaaaga
    11881 aaatcgatgg cgtattaaaa gagatgaaag aagatgggac gacaaagaaa atatctgaaa
    11941 aatattttca aactgatgtt tcagtcaaac cgaaagaaac gatggctgag tcggtgagtg
    12001 agtgatgtac tttcctctaa ttgatttttc cctcatgtgg acttcacttc ctttcgtact
    12061 gcaaggcttg gtatacacat tagggattgg cttcgtgtcg tttcttttag gaaatctggt
    12121 agggttgctt ttgacagttt taggtctttt ggattggctg cctctgaatg tatttattcg
    12181 tttttattta tcgttcttta gaggcattcc cgcactagtc ctgctgtttc tgttatattt
    12241 tgggttacct tatcaattaa gtgccttaac ggcttcagtc atctgcttta cgattaccag
    12301 cagtgcattt attggtgaaa tctatcgagg atcactagca ggcgtttctt caggtcagtg
    12361 ggacgctggt ttagcttctg gtttttcttt ctttggggta ttgaaatata tcgttttgcc
    12421 gcaagctttt cgaatctcag taccggcact cagtaacgta gctatggatc tagtgaaagg
    12481 tacttcgtta gcagcaacaa tcacagtgcc agaaatattt caaaaagcaa agatcatagg
    12541 cggtcggaca ttcgattatt tttctctcta tattttagtt gcactgattt attgggtcat
    12601 ttgtatcggg atcgaaaaag tgcaacagta tttagaaaaa cgttctatgc atcagtcaat
    12661 ctgaaaaata gaaatggtgc tagcgtggct ggcaccattt ctattttttg tttcttgtat
    12721 attctgccta gtattttaaa accatttcat gtaaaatgta aatcgaaaat atgttcggag
    12781 gtagaaaaca tgaagacccc gctaaccatc gaaatagaat cctcgttata ccaatattgc
    12841 atcgagcaag gcggcttggt tgtagaagaa gtcacgatgc ctgatgacca aggaattgta
    12901 gatacgctta gctgcttgac aaaatttgat ggaagccgag aatggcgatg ctatgagctg
    12961 aaagtgacaa aagcagattt tcgctctaca gccaaactgt catttgttgg acattataat
    13021 tattttgttt taccaagtaa actatatgaa gaagtaaaaa ctgaaatccc tacaggaata
    13081 ggtgttttag tgtatcgtcc gtacacggtt gaagaggaga tgcctgcctc tggtacattt
    13141 ctgattgcga aaaaagcagc aaagaaagag cttgctgtac cggaagaagc tttgacaaac
    13201 cgcttcatgg cttctttatt cagggaagta agaaaagcaa agcaaatgga gcgaggcgta
    13261 aaatattttc caacagatca attgtacaaa gaattgaaaa gacgttcaga aaatcaagat
    13321 ccattttcta acgaacgata ttatgaacgg tttttagagg aaatagctag tcaggcaatc
    13381 aatgagttgc aagaagaagt tatcgcgctt cagcatgatt atgaatattt gaggcaacaa
    13441 cagcagatcc gtcggttgcc tacagagcca ttggagtgaa aaatatgtac tatccttatt
    13501 taagaggaaa acaatttgac ctattagcat taaaagaagc actgtcgcga ggattgctct
    13561 ccaataaaat ccaaccagtg atcgaaccag tacgtgattc cgctacgctt aaaaatgtga
    13621 tcgaattatt tcaaaaaaag caccatccaa tcgctgtgat ccaaaatccc caagtaggac
    13681 aattcaaatt attcgatcag catgtgcaca gttggacagt caaagaaaat agctctgtcg
    13741 tccctgcaca gatcttaacg ccagaaaatc tcagtgaagt actggattca cctccagcgt
    13801 ttcttgtttt cgatggacag cactacccaa aagatacaga ggtgtggaaa cagttggcag
    13861 gagttgattc gaaatttttg ataccagata ctagtcgctt tcgcatctgg ctgcccgaaa
    13921 acaaaattgt gatccgtgac tcttttcaaa cgagaaagca tgttgaaagt tatgcagata
    13981 aaacagatga cttttttagt gatgactatc tttttttcca cgcagacgga tatattggat
    14041 tttctgattt cacgatcgaa ggtagccgtt atttcgataa agggttccct agccgagcgc
    14101 ttgctatcca tctaacgtat attgatgctt acggcaatat acgcgtgaaa cattttgtct
    14161 ctgacagtaa cgactctgct aaagaccaag cattgaagtt tcttgaagca ggagataaaa
    14221 tgaaaaaatg gatcatgcgt caccatcatc aactcttgat tacttcagga atgattgaat
    14281 tattggctct ttaccagcaa caaaaattcc ctggactggg agtgttgaaa aagtggtcac
    14341 ttatgcatca tttggaactt attagtcacc tgctagatca tccaaaagat tggcttaaat
    14401 attattcaaa agttcaggaa ctatatgaag tgatccaatg attagataat gtaaatgaaa
    14461 aaggaataga aaagaggaaa ctggatgtat attcggcaaa caaggaaaga agaagtagaa
    14521 gaagtggtag aaatcattaa tgccgcagcg gatttcattg cttcgcaagg ctctcctcaa
    14581 tggcaaaacg ggcagaaacc aacaaaagaa acgattttga acgatattga aagaaaagaa
    14641 agctatgtat taatagtgga agatcaaatt gctggaacag ctgcattagt ctcaagcgtg
    14701 gatcccgttt acacaaaaat agatggtagt tggaaagaat caactggtcc ttatttatcg
    14761 attcatcgtg tcgctttagc agaaagtact cgaggaaaaa aactagcaaa accgttctta
    14821 gaacatttgt tgacgattgc agccagcaaa aatatccaag atatccgtat cgatacttat
    14881 ccagctaatg aaccgatgga aaagaccatc tattctgctg gattcactta tcaagggatg
    14941 attgaatttc cttttgaaca cggcgaaaga aaagcatatc aaataatcat caattgacta
    15001 taaaatcatc tgagcaaaga taaagacatt aacactactg aaaaaaacaa tcatatgata
    15061 gccaagaggt tgtaacagaa gtggccagct tcaaaaaaca agaaagaatt tccgaaaatg
    15121 cttctcaaat ttttgtggaa ttttagcgta ttttacgaaa tagctgcttt ctgaaactcc
    15181 cttcatcgag aggtcgtgac atagcttttg tcacgacctc tcggcttatc ctacgagtat
    15241 tattcgatac atttatatca ataatccttt ttttatttta tctgcagctt cttggaacgc
    15301 tccatcgttt gacacgacta aaagtgaggg gatagctttc aaacgtttca actgtgctaa
    15361 atcttctaca taaaccgcca gtctggctga aatctgttca gatgaatctc cacgtttttt
    15421 caggcgcgat tgcaatgttt ctttagagat cgtaataaag ataggacgga ctaccgtccc
    15481 gaaatactca tataaatgcc aaaaaccatt tgctgtcaaa acggcgtaac aatttcctac
    15541 tgtcagtttc tttttaattt ctgctttact ggaaccatat ttatgttgag catattggtc
    15601 ccactctgca aactcatttc ccttgatcat ctgattgaat gtattgtcag aaacaaaata
    15661 ataatcctct tgatcttttt ccccaggtct tggtgatcga ttcgtgtaag taatgatctt
    15721 ttgttccggc tgaaaaatag cttctgcgag tgaagttttt ccagaaccag aaggaccgat
    15781 aaatacatag caataattca ttgtcatttc tcctttgatg attgaacaag tgaaaaagct
    15841 ttggaaaata tttccaaagc ttttaaaagt aactttgctt ttttgtatat tataccagca
    15901 agccagtcac aatagcactt aaaaagctga ctaaagtcgc tgttagcaaa attttcaatc
    15961 cgtgttttgc gacaagattt cctttttctt catttaatcc cttcatcgca ccagaaatga
    16021 tcccgataga agagaagttg gcaaatgaaa cgaggaagac agatataatt gctgttgtac
    16081 gctcagtaaa atggaaatta ccatttgcta attctgtcat agcaacaaat tcatttgtaa
    16141 ccagtttagt cgccatgata ctaccggcat ccactgcttc tttccatggg attccgctga
    16201 tgaaggcaag aggggcaaag atgtaaccta ggatctcttg gaaagtgatg ccgaatatcc
    16261 cgtggaagat agcattgatc atggcaatca aagcaacaaa accgataagc atagcagcaa
    16321 ccgtgatcgc cacatggaaa ccatcaagaa tgtattctcc caacatttgg aaaaatgtct
    16381 gttctttgtt ttcttcaatg atcagctcat cttctttttc ttctaataca tagggattta
    16441 caattgaagc cagaatataa ccgccaaata agttcaaaac aagggcagtg atcacatatt
    16501 tagaatcgat cagtgccata taagatccga cgatcgacat agaaactgta gacatagccg
    16561 atacactcat ggtgaacaaa cgtttctcag ataggaaggg taattccttt tttatggaaa
    16621 taaaaacttc cgattgtcct aaaatagcag aagctactcc attataagat tccaatttgc
    16681 ccatcccgtt gattttactt aatcctagac caacagcttt cataaataat ggaagaatct
    16741 tgatatagcg tagaataccg ataatagcag aaataaatac aattggcatc aatacggaaa
    16801 gaaagaaagg tgcacttcct ttatttgcta caccggcaaa aacaaagtca acaccttgcc
    16861 cagcaaaagc aagaagatga tcaaacattt ttgccaatac agaaacaacc gctgtaccaa
    16921 aagttgtacg taataatacg aaaccaagaa caagctgtaa aacgaacatc acaattagtg
    16981 gtttcaattt gattgctttt ttgttactgc tgatcaaaaa agcaagccct aatgtgacaa
    17041 ataaaccaat aatacctaat aaataactca aataatcact cctattccaa attccgtttt
    17101 attttacctg ttttgcaaat aaaatactaa tctttagtga aattttcatt acttttttag
    17161 agtgaaacgc attcattttc cattagaaaa taaagcgaac aaaataaaag gtttaaaaag
    17221 tgtaacgtct tagtaaagtc tataaaaaaa cgaaactatc gattaagcta atagtttcgt
    17281 tttcaacatt tataagtaaa ctttttctat tggtaatata gattttcgga agggtagaca
    17341 ggtgcgcagg taatatcgat tcctagtttt ttcaatgttt gttcatcatt tttattgacc
    17401 atcacagtag agtgggcttg tgctccctca agttcagcta gtttatcata agcaacttga
    17461 gcgattggat tcgttactgc actgattgat aaagcgatca agacttcatt gatgcttagt
    17521 gcatccaaag aggaatgaag tgtctttgat ttcaatcctt gtattgtttc aagaactaat
    17581 ggtgacaata aaggaatgtc atctgcgata tgggctaaat gttttactgc atttaagata
    17641 actgccgctg acgcatccat caatgaactt gtacgcccgg taatgatgac ttcatctggt
    17701 aattcgattg caatcacagc agaaggatct tttgtaggac tttgcgcttg aatcttatct
    17761 gcatagtcat gggcagggac taccacgttt cggtcttcag gacgtaaacc gacttcttcc
    17821 ataatcagtt tgattcgatt aactgcttct tcgtcgatca atcctttttt gaagtcgcat
    17881 tctgtttgga aataacgacg gatgatttct tgtttagagg cttcttggac aacggtatca
    17941 tccacgatcc caaatcctac acgattcaca cccatgtcag taggagattg ataaacagat
    18001 tctttaccag taattttttc aataatacgt ttgattacag gaaatgcctc aacatcccga
    18061 ttatagttca cagctacttt attgtaggca tcaaagtgga aggaatcgat catattgaca
    18121 tcttttagat cgactgtggc tgcttcataa gcaatgttca gcggatgttt caacggaaca
    18181 ttccatactg ggaaagtttc gaacttggag taaccagctg catgtccttt ttggctttca
    18241 tgatacagtt ggttcaagga agtagcgagc tttccacttc ctgggccagg agctgtcact
    18301 acaacgattg gtttagttgt ttcaatatac tcattttttc caaaaccatc ttctgagacg
    18361 attttttcta cgttgactgg atagtcttcg atttctgagt gtttatagac cttgatgttt
    18421 cgtctttcta gtttattgat aaagactttc gtggcaggct gtccactata gcgagtgata
    18481 acgacactgt tgattaataa gccataacca cgtaattcat cgattaaccg caagatatcc
    18541 atatcataag taatcccata atcaccacgg atcttattac gttcaatatc tccagcatag
    18601 acgcaaatca gtatttcagc ttgatctttc aatttttgca gcaatttgat tttcgcatct
    18661 tcttcaaatc ctggaagtac acgtttcgca tgcttgtcgt caactagctt cccaccaaat
    18721 tctaaatata atttgtcata gtggttcact cgttccaaaa tatacgctga ctgctcttca
    18781 atatattttt ctgaatcaaa accaattttt ttcactaggt tccctcctta agtactaaag
    18841 cattttttca tttttttgag gattcgtcaa ctggaagagc gaaattcctc aaaaaaacaa
    18901 agaaaatggt ttaaaccaac ttgtctagat agttattcat tggtatgata gtttttgata
    18961 aggaaggtgc tacatgaaaa aaagacagaa aaagaaaaac gcatataaac actatatccg
    19021 ttcgattttt accggttatg aaagaatgct tgaagatccc gaattggaac aattgacgtt
    19081 tacttatcta aacgaagaaa cgcagctaac tcgtgatgag catcagcgca ttcatttcac
    19141 aacaagagat ctgcctagta aataaaaaat aagataaagg tcatcggatt cgattctgtc
    19201 ctatcagatg gaaagactag ttgatcgaaa aaaaggctgt accaaatttt ggtgcagcct
    19261 gttttatgga gcattgcccg ctctttttta taacaatttt ttacagaaaa tggtactatt
    19321 gaagtattaa taagtgaaaa acagatgagt aagaggaggc aagtagtatg aaagagccag
    19381 atacaaatgc aaattatgag ccagacgaac tagaggagac gaatgcggat caggatacag
    19441 gacaagaatc gtcttctttt tacgatatca aagaaaaagc aagcatgaat ctttcccaat
    19501 tattagaaga cttacaggct tttgaaaaag ctgtccgcga agaagatatc cctgagatct
    19561 accgtttata caatggccga ttgaatgaag aattaaaaag aagctcaaat gaaaatcacg
    19621 agatcgatca attgctgatg cgtaagatcc atgatcgttt tttacaggaa tttcctttta
    19681 tggaacaagt agaaaacatc tcgccaacga tgagctatta caaaatcggc acttattacc
    19741 atgatcggcc cacggtcggt attgatgcaa gtctgccaga aatttttgtt ttgcctaaga
    19801 tcgatgaaga gtgggagaaa tattcccaac cggagtcaac ggactatgac aaaaaaatca
    19861 atgagctaga cgcaaaggtg atcactgctc agacagaaat tgaacgttta gatgaacaaa
    19921 taaaagaaat caatcgacaa atgactgatt tgaacgacaa taaaggattc ttgaatcgta
    19981 aaaaaatcga ggaagaaatt caagaattag agaagaagaa gcaagtacta tctaatgaaa
    20041 aattaggctg gcttccttat attgagacgc ctgaaacaat ccagcagcaa aaagaagcat
    20101 tgaagcaaga agcaagagca gaccagcttc gtgcagccat cgtagaaaaa gaacaaaggc
    20161 aaatcagacg ctatttcggt agcaaagagg gattcggtca agcaatccat gaattcttga
    20221 tgaattatct tggcaatgag aatcctaata atcaatctaa tgaaggaggt tccgagtatg
    20281 aatgaacacg atgaagcaga atggacaaaa aatgaagaac aaacagaaga tcatgaagaa
    20341 acgaacactt cattggaaga acaaacaagt aatcctctgg atgacacaga agaaatccct
    20401 atgctagagg aaacaaaaat acacgaaaaa gaaaaagtaa ctccgacgaa tacgaatact
    20461 gcagaaaaca cccatgctta cgaaataact gaaaatcccg ggtatttaga aggaaaagag
    20521 caagatcatc aagatatcgt cagtttgatt gaaacagcgg aagcagaatt agcggattta
    20581 cgtatgagaa aagatttgat cgaatcagat tttccacaat tattagaatc atatgatcgt
    20641 tttatattga atgaagaacc aatctccacg atcggaaaga caagcctgat cgaatacttc
    20701 acgtatgaat tactgaaacc gttgaatgat attggtctgg aacggtcaaa ttcagcttat
    20761 gacacatgga aaacaagaca tttttcgatc aattatacgt tgaatggaca aaacgaatta
    20821 gtcttttcct ttgtattacc gaccatcgat caacgatacc aagtggaatc gatgagtctc
    20881 ctaaacgtat caccagagac aatggaaatc aatgtccaag atgaccgggt gctttctttg
    20941 atccgacact ggtcagtgga tcgagtattc tctgcagggc agatcacgat attcaatcac
    21001 aaattgaatc aattattagc ccatgcaaga acacttggtt ttaccgttaa tcagacatta
    21061 ctagacaata cgaagccact tcgtttgaat cttcagagcg aatttgaatt gacgaatgaa
    21121 gtattggatg atatttttat cgtggcaatg aaaaatccat cctacgatat ggaaaagatc
    21181 aacgaaacta cttatcaagt acgattagat aaaggacagt ctttaacgat aacaacaaac
    21241 gaaaaagatc aaacagtctt gtcgatctca tctggagact acccacgatc tgtcattgat
    21301 ttctttatca attacgaatt cttggtccca ttaatggtga gaaagtcctg attggataag
    21361 ttggattacc agatgtcatt cagataattc tgttccaaag gacttgcggc agttatccag
    21421 aataattgtc acaagtcttt tctgtatgta ctaaatggac gtgaataact tgctgatgaa
    21481 ggagggattt cattaggaat gtccacaaat aaaatagcac agcttagctt attgtctgct
    21541 gcttgtgtag caggtaggat cgcttttcaa tttattccga atttccagcc aatgactgct
    21601 atttttttat ttatagtact ttacttaagc ttaaaagatg cgttaattgt catgagtctt
    21661 tctattgcga tcagcagttt ttattttggt gttggaccat gggtcatcgg ccaatggatc
    21721 agctatactg ttgttctgag tttgttttca atcgtctgct tgagaaaaac agtgcaaagg
    21781 aatctatggt taaagggagt ctgttttttt ctggcgggca tggtttacgg aataggtatg
    21841 accgtttttg atacgctgtt atatcgtttg cctcaacctt ggatctatta ccttcaaggg
    21901 gtgtcgtttg atttgatgca cagtataggc aatgttgtgt tctttcttgt ttttctgcca
    21961 gtcgtcaaac gtttttacaa tcattcagga ggaaaaaatg aaaaaaataa tttatagttt
    22021 gcttgcaatg agtgtcagta tactcatttt gacaagttgc agtacaacct atacacaatc
    22081 ggcttccaac caaaaggagg aagtaaaaac atcgcaaaaa gcaacaatca cgttacaaga
    22141 aaatggtcaa aacttttcgg aaaaagaagt gttcttcaaa aaaggcgatg atttactgag
    22201 tattttgaaa cgaaattttg aagtcaaaga agataacggc ttcatcactt cgcttgaagg
    22261 gcactcacag gatgagaaaa atcaactcta ttggatgttt actgtggacg ggaaaagtgc
    22321 tactactggt gccaaagaga tcaaattgaa tgatggacaa tcggtcattt tcaatttgtc
    22381 taaactataa gtttcataaa aaactgttaa agggcgaata ttgccataat tcagtgagtt
    22441 cagttgatga aagaaggaaa ataacatgga aaattttaaa aaaagcggaa tgttttccgt
    22501 aattgctgct ctaggggcta atattctagt tgctatcagc aaatttgtcg gctatgcaat
    22561 cagtggcagt gccgccatgc taaacgaaag catccacagt atcgttgatt gcagcaatca
    22621 gattttgctg ttattcggtg ataaacaagc caataaagga cagagcgagt tgcatcaatt
    22681 tggtgaagga cgagcaaaat actttttcag tacaatcgtc gcaatgatgc ttttcttcgg
    22741 cggtggggca cttggggtga tggaagcttt tgaaaagttg acgcatccat cccatgaagt
    22801 gggtaatccg atcattgtta tagcaatact gttatttggt ttggtaatcg aaggaagttc
    22861 gttacgcgtg gcaatgaaag aaatcaaaga attgaatact gaaaaacttt ccacgatgcg
    22921 ctttttgcgc gaaagtcgac acagtgaaat actgattatt tttacggaag acttgtgtgc
    22981 tgtaatcggt ttggtcttgg ctttagcagg aacaatattg acttatgcaa ccgggattgc
    23041 tgcatttgat gcgatcagcg gattgctgat cggtttcttg ctgatgggag ccgccatttt
    23101 cctagcaaaa gaattttata gtctgatcat cggtgaaagc gtgacagcaa gcgatttatc
    23161 aaaaatcaag ttagcttttg aacgtcctga gatcagtcgg ctgatcgata taaaaacagt
    23221 tcatttaagt ccaacggaga ttttagttgc agcaaaagca gatatcgttg gtaacaaaga
    23281 gaatgaagct tactcggtca tcaatgatat tgaaaaaaat atgcgtcagt ctcttcctga
    23341 caagaagatg tatatataca ttgaaacaga taaatatgac ccaaattatt ctcgtaaata
    23401 aaacgtttat tcatgaattg aagcaaaaat gagaaaaata taatcttgtt tttaaataaa
    23461 acggctacaa tttagcgaat ttatgttaca atgagtccgc tgtttatttg ttgtaattaa
    23521 aggagatatt ttaatgaaca aaaagaaaag tgcgccatgg cttcaagctt tgcatgcagt
    23581 gatgccactt tgtataagct atataccagt aggattagct tgcggagtat tgttgcaaaa
    23641 agttggattt gatcctctct tatcaggact gttatcgatt ctggtctttt ccggtggcgc
    23701 gcagttttta gttgcatcta tgcttacgac gcaagcatca tttgcaacga cattattgat
    23761 ggttttcttt ttagaattac gctatacatt attaggagca agtcttgccg gttttatgaa
    23821 aaaagaaaag cggacgtttt tggctgtctt ttctcaatca ctgaatgatg aaaactatgc
    23881 ggtgaactac ttaaagtttt caactgaccc ttcctggaat aaaagaaagg ctttatatgt
    23941 caattggttc tcaatgactg catgggccgg tagcaatatg atcggtaatt ttttcggctc
    24001 ggtgattcga gtagatgctg accttgtcca ttttgctttg acggccatgt tcattttcat
    24061 gtttatcatg cagatgaaaa gcgcactact gatctttaca ggtttattct caggcgtatt
    24121 aggggtcgta ttcatggtat tattccaaaa tactttcgga ttgattgcag caacagtcat
    24181 tgcttcattt gccggattta tgctggagaa ggcattcaaa aaagaaaaag ctaagaaact
    24241 aagagaccgc ggtaaagatg tcaatgagcc gctcttcaac ttcccggaac aggaggccaa
    24301 tcaccatgac taaactatat ttgctacttt tagttgcttg tctgttcatc gtggcatata
    24361 tcccgcgctt gttcccgatg ctttatttta ctcatcgaaa agtgccatcc tggttcagtg
    24421 actggatgaa atatgtaccg gtggcattat ttgcggcgtt agcttttaaa gatgttttca
    24481 ttactcatga acatctggat attgcttgga atatcaaaat agctgcaatg attctagtag
    24541 caggcgtggc ttacaaaaca cgttcgatgg cgttgtcagt tttgacagga cttgcgtctg
    24601 tctttttgct gtctatgctt taaactgttt tattttatta aaaacagaga ttgcgacata
    24661 agttgttcaa actgatctat ccgaactgca tttgcaacag tcaattctgg atgcaagttt
    24721 attcgtaggg ataaaaaaga ttagtgttgg cttatgatgc aagctctttt ttattttagg
    24781 aattagagag ggatttgtcc ttctatgatt ttttgctata atagggaaga tgtttatttt
    24841 atgaaagaat agttgaggga atttaaatga ataaaaaatt gatgcaagga acattttggc
    24901 taacctttgc taatcttcta tgtaaagttt taggggtagt ctatttgatt ccctggctaa
    24961 gtatgatggg gaacaatcaa gatggtatgc ttgctacgac attgtataat gttggctatc
    25021 ttccttatgg attgttcctg atgcttggta cagtcggatt tccaaatgcg attgcaaaaa
    25081 aagtagctgt agccacaaaa gaaggagata acgctgcttg tcggctgatt tttagaagta
    25141 cgatcaatat catgtttgtc atcggtatcg cctctgctgc actgatgtat ttatttgctc
    25201 ctttactagc cggaatcagt ccgattgcta acgttaataa tgggatacta gctatcagaa
    25261 gtttatgccc atctttgatt gctatcccaa ttcttagtgc aatgagagga tatttccaag
    25321 gaaaaaatga tctgcggcct tatggaactt ctttgattat cgaacaggct gtgcgagtga
    25381 tcgttatctt ggctggtact tattacttgc gcgtattgac tgacggaacc atattagaag
    25441 ctgtattgat cagtacagtc gcttctttct tcggtgggtt agctgcgatt atccatatgt
    25501 atatcgtcgg acgcagaaaa gacttcttcg aacttaaaga ttttttgatt tccagccgtt
    25561 atttcgaaag agaaaaccga tctgcctctg tctctatcat tcgggaaaca ctaccgttta
    25621 tttttgtcgg ctcagtcatt actatcgttc aaatgatcga ccaagtaacg atgaaaccac
    25681 tgcttcactt tttccgtcca gagatcgcag atcagcaact ggaattctta ttcagccgtg
    25741 cttctgtcaa tccaaataaa ttgacattga tcctgatctc catggtagga acagtagcta
    25801 taagcagctt gccaatatta agcacgatga agaaaaaaga tcggatacaa atcgaaaaaa
    25861 cagtaggaga tagtttttcg attgcgttat tgattcttct tccttcattg actggaatgt
    25921 ctttattagc tggaccacta tacacattgt tttttggcta tgatcctgaa agtgtcggat
    25981 atttccaaat ggctttgcta gcttctttgt ttttctcttt attcacgatc ctatctacga
    26041 tgcttcaatc tttgaatcac catcgttttg cgatccgatt aactgtagaa gcaattatcc
    26101 tgaaagtcat cttccaagta atcggattag gattagttgg cggatacggg atgagcttat
    26161 caaatggctt agcattcggt attgtctttg ttcgcggcta tcactttatg tgcaaagaat
    26221 accgaatagc acctttagca aagatcagca attttttctt aaaaactttc cgctccaccc
    26281 tgattatgct tgcagtatgt tttgccgttt tctttttgct gaatcagcga ttgtcaatgg
    26341 aatcaaaaag ctatgcaatg atttattgtg tggctgtagg aagtattggc ggcttagtat
    26401 ttttattttc tcaattcgga aaaaatgggt ttcgaatgtt gaaaaatgtt aaacatcata
    26461 gcagaagcaa agaataggtt aactaatcgt tttttgctgt cacttgaaaa acggcaactc
    26521 gtatgattac gcttgtcgtt tttctatttt ccaattttag tttcatatct aatcttactt
    26581 atgatatagt tataaggtct ggaaaaattc tttgaaacga ggaaattaaa cacatgaaac
    26641 ttgcagtagt tacagatagt tcggcatatc tgccggaacg tatcaagaac catcccgact
    26701 tattcgttat tccaatccct ttgattatgg atgggaaaat ttataacgaa ggaatcgata
    26761 tcgaagcgga tgaatattat ggacttttaa aaaacagtaa agattttcct actacttcac
    26821 aacctgtcgt aggggaagtt ttgggtttat atgaggaatt gaaagagaaa gggtatgaca
    26881 cgattttaag tatccatctt tcttcaggga tttctggctt tgtcaacaca ttatttgcga
    26941 tgaaagatga catcagcgga gtaacggtca ttccttatga ctctaagatc actagtatgc
    27001 cgatgggaca catggtagaa gctgccttag acttaaatga tgaaggtcag tcccttgatg
    27061 aaatacttgc acatatcgat cgaatccgag ataataccta cgcttatctg attgtagatg
    27121 atttgaacaa tttagtacga ggaggacgtt tgacaaatgg tgcagcgctg attggcagct
    27181 tattaaaaat caaaccgatc ctgacatttt ctgaagggaa aattgtttta ttcgaaaaaa
    27241 tccgttcaag taaaaaagca tttgctcgag ctgaagaaat tattggtaga agaaataacg
    27301 aaatacaacc gccagtcaaa ctttacgtga tacatgcaaa taatttagaa gtagcgattg
    27361 cggagaaaga gaaattgcag aaaaaatatc caaatgcaga aatcgaaatt ggtcatttcg
    27421 gaccagtcat tggtacacat ttaggtgaaa aagccatcgg cattgccatt tcagcgcaat
    27481 aattcctttt attcaaaaaa cataccaaaa atattccaac atcatcttgg actttctaca
    27541 tgttactaat gagcgtgtaa aagaacgaga tggtgttttt atttctatca atgaagctct
    27601 ttttctttgc aatcctcact tcaatcctct ataatgtcgc atatagttta gtaaatgagt
    27661 aaactactaa tttacttatt tactaaaaat aaaaggatga ggagttaaaa taatggaaat
    27721 accaacaaat ttaaaacata aaccagtaat cgttgtagaa gactatgata aagttgatgg
    27781 aagaaatgca ttaaatacgg atgcaaaagg gctttcactg ggccttgctc agtggaatga
    27841 tcgtggaaaa gtcgatatct caggcaaggt atggcgacat actggagaaa aatggtcacg
    27901 acagtcggaa gaattaccaa tcacacgtat tttagattta gcgatcttag ctgcacaagg
    27961 aagtttatac ttccaagatg cttatcgtta tgaaaaattt tatgatccag aaaatccagt
    28021 cgtagacatc attggattac aagggaaccg aatgacggta gaagtagata ccaaaaatcc
    28081 aaagatcgat gaagacatca ttttgtatta cgatacacta caaaaagacg gagaactgat
    28141 tggggaacga ttccgtattt taaaacgtct tttagatgag ctaggatatt aatagacaga
    28201 gatgatcaaa aggggaagag aagcatggag aatttctcac taacagaaaa ccgaaccttt
    28261 tcacggctag aaaagcaaat gatctggaag aaaccgaaaa gccatcggac aagtgcagaa
    28321 gaatggcgga tcgcctccga aatcaaaggg aattggcaag atcctgaaat gaaaatcagt
    28381 aacgtacttt tggaaggaga tgccgggtcg gggaagacac aattagccaa agcactatct
    28441 gctgatctgc aacttcccta tacaaaagtg acctgttttg cagatatgga caaatcagat
    28501 gttttcggag ccttattacc tgtagtagat tctaataagg aacaagacca agaattacta
    28561 gaagctattt accaaacaga tacacttgaa gctgtactga cactgatcca gaaccattac
    28621 ggcattgatc ctttttctgc taaagaaaaa ttagcagagc tagttcaaag gatcgaagct
    28681 gattatccag atgcaatcca ttacagctat tacccttctg aaatcgttcg agcgattgaa
    28741 aaaggatatt tattagagat tcaagagccg actgtcatcc gagatgcctc tgttcttgtg
    28801 gcacttaact cagcgctcga accaaatggg atgttgaatt taccgacagg aatcattcgt
    28861 cgccatccag attgcgtcat tgtgatcact acaaatcgaa attaccaagg aaatcgccca
    28921 ttaaatgaat ctttaagaga tcggatgcaa catgcggaaa aaatggatct tcctgcctta
    28981 gaagtcatgg cagaacgtgc gatggcgaaa actatgatcc aaaaacaaga acttctctta
    29041 aagatggcag agattatccg tttgttggat gaaactgcaa aagctaacgc catcaaaggt
    29101 gtagcaggaa tgcgttctta tttttattgg gcaaatacct ataagcaagg acaagattta
    29161 ctccagtcga tttatccaaa agttttgtat aaattatcca ctgattcaga tgaattggca
    29221 attcttgaac aagctttgtc tgacagtgga ttgctactgg aacttcagga catattgaga
    29281 aaagaaaaat ggggtcatcc tcaatctgag catacaaaag gaagaaccat tagcgccgaa
    29341 gaagcaaatg aacgaaacat cactgaaaat gctgaagaaa ttctgcgctt agatgaaaag
    29401 aaatcgttgt ctgctgaaga accaaatgag ggagaagaga aacaagaaga aacaatagaa
    29461 aaaaacgaga ttcctcctca aatgcaaaaa gaacgcagta gcgatatcga aggaaaaaat
    29521 caaaaagaac aacaaacagg agatgattct caagaaatgt ctgcttctga catggaagca
    29581 catgataagg aaatcaagaa gcaactgaat aaagaagcgc gacaaatcat gaaggaaacg
    29641 attcatgaaa aagaaggtct catcgttcac cgaccagctt tccaaggaaa aacgccggaa
    29701 gcacgtgagc tacacatgca agtaatgcca atagtagaat cacttagccg gcaaatttta
    29761 gagttactgg ataatgaaca aacagagacg taccaaaaag gaaaatacga aggtcagcgg
    29821 ttcaatgcaa gcaaagtagc atatggtgat ttgagaaatt ttgacaaaaa gaacccgcca
    29881 catgagcaac cttctttagc ggtagctgta cggatcgatg aatctgggtc aatgatacgg
    29941 gatgatcgga tcgaagcggc aaaaaaagca gcgattgcca ttgccgaatt ttctaagaaa
    30001 gtcgatatcc cattgttgat ttacggagat acagcagacc gttcgtcaag agaaaagact
    30061 tctttgtttt cttataaaga atttgaagac ggatttcaat ggttaaatga aaaattagtc
    30121 actatgaaac caaggcaaaa caatcgtgat ggtgctgcac ttcgtttagc agcagaaaaa
    30181 ttaaacagac aatccgcaac aaccaaactg ttgcttaata ttagtgacgg tcagccaaaa
    30241 gcgttgccgg attatacagg caaaaaagca aaagaagata tccagtctgt tttgaaagaa
    30301 tatgaacgtc aaggaatcct atttgtctcc gcagctattg gccaagacaa agaagaaatc
    30361 aagtcgatct acggtgaaag tcgttttgta gatattactg acttgaatga atttcccaag
    30421 caaatgattc aactgattgc ccgctatcta tgataaaata aagctcaacg aacgcactta
    30481 gcagattcgt tgagcttttc gtttctagtt accggtaaca aggatacttc taaaatagaa
    30541 tcggttataa tcaagaggga ccgttaaagg agtgaaacga tatgatacaa gaacaaaaat
    30601 ggtggaaaga agcagtaggc tatcaaatct atcctgctag tttcaaagat agcaatggcg
    30661 atggtcaagg agacatcaat gggatccgtg aaaaacttaa ttatttgaaa gatcttggta
    30721 tcggctttat ctggatcaat ccgatctatc aatctccatt tgtcgataat ggttatgacg
    30781 taagtgacta tcaagatata gaagagagat tcggaacgat ggaagatttt gatttattgc
    30841 taaaagaagc acatgaatta gggatcaaag tgattatgga tcttgtcatc aaccatagtt
    30901 cggatcagca tcaatggttt gaagaatcga aaaaatctaa agaaagcccg tatcgcgatt
    30961 attacatttg ggtcgacggg gtagatggca aagaaccgaa taattggaca tcgattttcg
    31021 gcggttctgc ttgggagtat tctcatgaaa ctggacagta ttatctgcat gtttttgcaa
    31081 aagaacaacc agacttaaat tgggaaagcg agaaattgaa agaagaacta ttcaatatga
    31141 tccgatggtg gttggataaa gggatagatg gatttcgttt agatgcgatt tctcatgtaa
    31201 aaaaagatga atattcagta aaagccacag agaacccttt ctcacccttt caaaatgtct
    31261 caggaatcga agaacatttg accgatttga aacatgtttt tgaagagtac gatatcatga
    31321 ctgtcggaga agcaagtggt gtcactgcag aagaaggacc acaatgggta gggaaagacg
    31381 gttatttcga tatgatattc gaatttgatc atatccatat ctggcagcaa gaaaaagaag
    31441 ggcaattaga tgtccttaaa ttgaaacttg cattaagcgc ttggcaaact tcattggatg
    31501 gtatcggatg gaacgcttta tatatggaaa accatgatgt tcctcgtgcc gtttcagtgt
    31561 ttggagatac tcgtcccgat ttttgggcta tgtctgcaaa agcaatcgct atgatgtatt
    31621 tctttttgca aggtacgcca tttatttatc aaggacagga aatcggaatg acgaatatgc
    31681 cctttgaatc gatcgatcaa gttgatgcag tagattcgaa aagactttat aaaaggctgc
    31741 ttgcggaagg taagacaaga gaagaagcat tggatatcat tcgtgaaaca acgagagaca
    31801 acagcaggac acccatgcaa tggacttcgg aacaatatgc tggtttttct acacatgaac
    31861 cttggctgat cactaatcca aataccaaaa cgatcaatgt agaacaacaa gaatatgaac
    31921 cagagtcggt cctgcaattc tataagaaca tgatccgcat tagacaaacc aataaaggtc
    31981 tgatatacgg ttcttataaa gaatatcttc atgaacatcc acaactttat gtctatgaaa
    32041 gatatttaga agatgaacaa tatcttatta tggtaaactt aacagaatca cttgctgact
    32101 atgaacttcc aaaagaagca gatcagtcct ggacattact cttgtcaaat tcctctagcg
    32161 gagaatttga agctaaaggc atactagcac cttatgaagc tcggttatat aaaacaaaca
    32221 agtaaaactt cctcgttaaa gttcattagt tggactttgc gaggaagttt ttcagattgc
    32281 tctgtcaatt actcatttca ataattcgct ctaggaccac cgatatattt aggacgagag
    32341 cgaagggctg tcacgtgagc ttgtccaagg gttttcaaac gaggtctgac aggtacttgg
    32401 acatgtttga tatgcatacc gattgatgtg tcaccgatat cgataccggc ttgtccaact
    32461 acgtgttcca cttcgacagg atcattgaac tggtcaaaag cagcaacaga acaagcacct
    32521 ccagcatgta gtgcaggaac aacagagaca atttcgaaat tttttgcttc tgccactgtt
    32581 cgttctacta ctaatgctcg gttcaaatgt tcacaacctt ggacagctag cgcaatttct
    32641 ttaggatcca acaactcttt taacgtacgg atgatccatt gaccaacttc cgcactggaa
    32701 ttttttccaa tatgtcctcc aacgacttcg cttgtgctgc atcctaaaac aaaaagatca
    32761 ccttttttca agtgtgcctc agccaatatg tcattaacta tttcagtcaa ttggtctttt
    32821 aattcttttt ctgacaactt cattttctaa aaccttcttt ttcaattaat attgcttccg
    32881 tgcttgaata cgttgaagac ttgcagttat aggaaaggca agtcccattc caatcagatt
    32941 ttggaagatg tttcctggta tagaagccac tccagccggc caatcatata aaatccaacc
    33001 ggcgatacca tagccgatga tcataactaa gcttcctaat aaaagcccta atccccacat
    33061 acgagtagaa gatttgctga aagaaccagc aacatatcct tgtaacccgt ggatcactaa
    33121 tgaaaaaact gcccactgcg gatatcctga aatcaaatcg atcaatagac cactggaagc
    33181 accaacaact aatccacccg ttggaccata aagaaaagca gcggtataga tcccggcctc
    33241 gcataatgtc acaaatccat tcgtagccgg tacagggatc aaaattgtta atgaaagggc
    33301 aactgtcaac gcaatcagca tagcatagat cgttaattgc ttagttttca tttctcattc
    33361 ctcctttttg aataccgcca gaccagacac ttccggtatg atcgttcatc agcacgccat
    33421 tagaaataca atgggcaaca aattttttac tttctgctac tgctttggta aaatctgccc
    33481 ctaacacaag attcgctgtt attgcagaag aaaaggcaca accggccccg tttactgttg
    33541 tagtgtcgat ttttttatga gaaaatacct cataatgatc aattgaatag agaagatcca
    33601 cagcttcatc tccagccatc cttgtgccgc ctttgatcac aatgtttttt gttcctgaag
    33661 cagtgagttc tttagcgaga tggatcattt ctgttaacga agagggaaga gcttgaccag
    33721 aaagtaaagc agcttctgcc aaattaggcg taatcatcgt aacaagtgga aaaagttctt
    33781 cgatgagctg cgaaatatac aattgattga tcacagcttc agtttctttg aatgccaaaa
    33841 cagggtctaa aatgattggg atattttgtt ttgtttttat gaagtctcga acaaccatga
    33901 ttgcttcact tgaattcaat aacccaattt tgataccatc taatgaagtc attttatcaa
    33961 tcgaaactaa ctgtcgttta agcaattcca aagatataga ttccagcata aatcgctcat
    34021 taatatctag tgcccctata gaagtcaagg cgcttagtcc gaatagacca aaattctcaa
    34081 aagtttttaa atctgtttgg attcctccac cagcaaatgg atcagaaccg ccaattgtca
    34141 agattctttt cataacattt tcctccatga ataagagtat aacgaaattt tcaaaaaaga
    34201 aaaacagcca attggcatat ttttatctca tccaatttaa aaaaacaaaa agaaaagaaa
    34261 gaccaaaatg gatctttctt tccttaaata gttaggactt ttgtgatttt aattctttct
    34321 cgatttgaag ccgttgcact ttcattgtag ctgtccgcgg gattgcatca aaatcacgga
    34381 tgatagggac gttcaagcga ggaagttcat ggacttgctc ccaccatcga ttccaatcca
    34441 tttctttatc aggagctaaa gcaataatcg gttgtggaga atgattttta tcccttacaa
    34501 tcactacttc tgccaaaaaa tctaacgaat ctaataaaaa gtcttcaata gctaatgtac
    34561 tattgatcgt ttcgatcaaa tccacttgcc gatctttcaa aaataggtgg ccatgcttat
    34621 ccataaaacc gtaatctccg ctatcccacc aatcgccata aacattttcc tggaaccgtg
    34681 catcttcctt gtaataagtc aaagcccgtc ctttagacag taattgtata tgaccatctg
    34741 tgttttcagg taggacattc ccaactgaat ctgtaatccg agcgcttgtc atgtcttcca
    34801 gtccaacgcc catatctctt gcatcacttg ttttcaaact ctctagtgta tgagccttaa
    34861 gaatcattgg tccgcattca ctttggccat atacttgaag aaataccgca tcctgtgcca
    34921 tcgacgttcg caaaaatgtc ttcattgtct ggttgtttat agtatcaaaa gtggagtgat
    34981 agtatcggat acccgaaaaa gcttctggat gctccttagc agtaaaacgc cactgaacaa
    35041 agttattcgg atgggtctct agtgcaattg gccggtgagc ttccaacata tgaacaactt
    35101 ttgaactttg tgcgtttgct aaaggcatca taggaaatcc catggacatc aaagaagaga
    35161 taccaatatt gaatctggaa tgcactggag agatgtgaaa agcgattaat ttcttttccg
    35221 aaatttttgt gaaaactgtt ttttgccatt tcgtccgcca tcccatagaa tgagcagaat
    35281 gacaaataag tttagggata ccagttgtac cagaagtatg ggtcatataa gaaatttcat
    35341 ttttgtctaa aaatgattga gaaacagaca aagcaggttg ttccattaaa taaagaatag
    35401 gaatacccgt ggtgctagtt aattttaata tcctaaagaa tatctcaaag ttcctt
//
LOCUS       DAZKKW010000020        34687 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.020, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000020 DAZKKW010000000
VERSION     DAZKKW010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34687
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.020"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            591..1280
                     /locus_tag="ACG8PO_000980"
     CDS             591..1280
                     /locus_tag="ACG8PO_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFQ0206324.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1267..2475
                     /locus_tag="ACG8PO_000981"
     CDS             1267..2475
                     /locus_tag="ACG8PO_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFQ0206325.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2769..4040
                     /gene="serS"
                     /locus_tag="ACG8PO_000982"
     CDS             2769..4040
                     /gene="serS"
                     /locus_tag="ACG8PO_000982"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFQ0206326.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4589..6073)
                     /gene="guaB"
                     /locus_tag="ACG8PO_000983"
     CDS             complement(4589..6073)
                     /gene="guaB"
                     /locus_tag="ACG8PO_000983"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFQ0206327.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6578..7273)
                     /locus_tag="ACG8PO_000984"
     CDS             complement(6578..7273)
                     /locus_tag="ACG8PO_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HFQ0206328.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7363..8463)
                     /gene="ychF"
                     /locus_tag="ACG8PO_000985"
     CDS             complement(7363..8463)
                     /gene="ychF"
                     /locus_tag="ACG8PO_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002269933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFQ0206329.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8472..8663)
                     /locus_tag="ACG8PO_000986"
     CDS             complement(8472..8663)
                     /locus_tag="ACG8PO_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HFQ0206330.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8684..9574)
                     /locus_tag="ACG8PO_000987"
     CDS             complement(8684..9574)
                     /locus_tag="ACG8PO_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFQ0206331.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9561..10328)
                     /locus_tag="ACG8PO_000988"
     CDS             complement(9561..10328)
                     /locus_tag="ACG8PO_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFQ0206332.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10340..11053)
                     /gene="rsmG"
                     /locus_tag="ACG8PO_000989"
     CDS             complement(10340..11053)
                     /gene="rsmG"
                     /locus_tag="ACG8PO_000989"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFQ0206333.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11285..13186)
                     /gene="mnmG"
                     /locus_tag="ACG8PO_000990"
     CDS             complement(11285..13186)
                     /gene="mnmG"
                     /locus_tag="ACG8PO_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFQ0206334.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(13203..14600)
                     /gene="mnmE"
                     /locus_tag="ACG8PO_000991"
     CDS             complement(13203..14600)
                     /gene="mnmE"
                     /locus_tag="ACG8PO_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFQ0206335.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14899..15282
                     /locus_tag="ACG8PO_000992"
     CDS             14899..15282
                     /locus_tag="ACG8PO_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0206336.1"
                     /translation="MVALSKLYTFERRKYVSPFRSAALEEESDPRINDVVLSTGNINQ
                     KYIVRDIVFVVECLEADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQK
                     KRDGKYFLELFAYGTVIQFIQTTIG"
     gene            complement(15450..16208)
                     /gene="jag"
                     /locus_tag="ACG8PO_000993"
     CDS             complement(15450..16208)
                     /gene="jag"
                     /locus_tag="ACG8PO_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HFQ0206337.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(16226..17044)
                     /locus_tag="ACG8PO_000994"
     CDS             complement(16226..17044)
                     /locus_tag="ACG8PO_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HFQ0206338.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(17041..17397)
                     /gene="rnpA"
                     /locus_tag="ACG8PO_000995"
     CDS             complement(17041..17397)
                     /gene="rnpA"
                     /locus_tag="ACG8PO_000995"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFQ0206339.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17515..17649)
                     /gene="rpmH"
                     /locus_tag="ACG8PO_000996"
     CDS             complement(17515..17649)
                     /gene="rpmH"
                     /locus_tag="ACG8PO_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFQ0206340.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            18172..19506
                     /gene="dnaA"
                     /locus_tag="ACG8PO_000997"
     CDS             18172..19506
                     /gene="dnaA"
                     /locus_tag="ACG8PO_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFQ0206341.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19704..20834
                     /gene="dnaN"
                     /locus_tag="ACG8PO_000998"
     CDS             19704..20834
                     /gene="dnaN"
                     /locus_tag="ACG8PO_000998"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFQ0206342.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            21063..21305
                     /gene="yaaA"
                     /locus_tag="ACG8PO_000999"
     CDS             21063..21305
                     /gene="yaaA"
                     /locus_tag="ACG8PO_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HFQ0206343.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            21292..22416
                     /gene="recF"
                     /locus_tag="ACG8PO_001000"
     CDS             21292..22416
                     /gene="recF"
                     /locus_tag="ACG8PO_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HFQ0206344.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQIRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            22413..24359
                     /gene="gyrB"
                     /locus_tag="ACG8PO_001001"
     CDS             22413..24359
                     /gene="gyrB"
                     /locus_tag="ACG8PO_001001"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFQ0206345.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            24379..26850
                     /gene="gyrA"
                     /locus_tag="ACG8PO_001002"
     CDS             24379..26850
                     /gene="gyrA"
                     /locus_tag="ACG8PO_001002"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HFQ0206346.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDIAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKENQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            27086..27385
                     /gene="rpsF"
                     /locus_tag="ACG8PO_001003"
     CDS             27086..27385
                     /gene="rpsF"
                     /locus_tag="ACG8PO_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005226363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFQ0206347.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            27435..27947
                     /gene="ssb"
                     /locus_tag="ACG8PO_001004"
     CDS             27435..27947
                     /gene="ssb"
                     /locus_tag="ACG8PO_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFQ0206348.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            27973..28209
                     /gene="rpsR"
                     /locus_tag="ACG8PO_001005"
     CDS             27973..28209
                     /gene="rpsR"
                     /locus_tag="ACG8PO_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFQ0206349.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            28352..30325
                     /locus_tag="ACG8PO_001006"
     CDS             28352..30325
                     /locus_tag="ACG8PO_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HFQ0206350.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            30331..30783
                     /gene="rplI"
                     /locus_tag="ACG8PO_001007"
     CDS             30331..30783
                     /gene="rplI"
                     /locus_tag="ACG8PO_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFQ0206351.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            31057..32424
                     /gene="dnaB"
                     /locus_tag="ACG8PO_001008"
     CDS             31057..32424
                     /gene="dnaB"
                     /locus_tag="ACG8PO_001008"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFQ0206352.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            33204..34496
                     /locus_tag="ACG8PO_001009"
     CDS             33204..34496
                     /locus_tag="ACG8PO_001009"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFQ0206353.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT        10569 a         6744 c         6858 g        10516 t
ORIGIN      
        1 ggagtgtttc ccgattcgtt tttggctgcc gtagcaactt ttatatcata tcagagagac
       61 aagtaattgt caactctttt ttaaaagttt tttcgatttc gtttttcgaa atcttccagc
      121 aactcactta tcataactta tcgtttgtta tttgtcaaga acttttttga agtttttttc
      181 gatagaatca agcttcgcaa gtggttcttt tttaacgact cagttatctt atcatgtcat
      241 ttccaacatg tcaacaactt attttttatt atttaataag ttattggtgt tcttttgaac
      301 aaataataat atatcaacat cacatgtgaa agtcaataag aaaatgctta tttatcaaca
      361 tttctagctt acatgatcat tggttagttc tggtcctttt tcgttcgtct ttataactcc
      421 gagagacgta aaatcctttt gttttcagtt taatgttcat gttcttgtct tattcacacc
      481 aactagatgt ctcaccttct aaaattttcc ttaacaaagt gctgctttta tctatttctg
      541 cttttttttg ctagaataat agtaacaata tgactctgga ggatttattt atgaaaattt
      601 tgattgctga cgatgataaa gaaatcgttg aattattaag tatctatgtt cacaatgagg
      661 ggtatgaagc agtaaaggct tatgatggga aagaagcatt atctaaaatc agaacaatcc
      721 ctgatattga attacttatt ttagatatca tgatgcctga gatggatggg atgcaagtag
      781 tgaaagagct acgaaaagaa tcccaaatac caatcattat gctgacagca aaaactactg
      841 atatggataa gatcaaagga ctggtcgctg gtgcagatga ttatgtgacc aagccattta
      901 atccgttaga agtaatggct agagtaaagt ctttattacg gcgtacaaaa atgcagattg
      961 ccccagatca gcctgatgta ttagaagtaa atgcattaat catcaataaa gactctcatg
     1021 aagttaaaac aatcgatggg aaagaaatcc agttaacagc tttggaattc ggtatcctct
     1081 acttgcttgc tagtcatcct aatcgagtat ttagcgcgga tgaaattttt gagcgtgttt
     1141 ggaaacaaga aagtattgtc tctgctaaaa ctgtcatggt tcacgttagt catttaagag
     1201 ataagattga agaagcaact ggtggtgaaa aagtcattca aaccgtttgg ggagtgggct
     1261 ataaaattga cgcaagatag taaacgagca actgaaaaac gccgtcggat caatttgact
     1321 tcaaaagaaa tcagcgaatt gttagcggaa ggaatcatca cgattatcct tttgcttcta
     1381 ttaaatgttt ctattctggt tgttatcagt tcggtgatca acagttcacc ttctttgacg
     1441 aatgcgattt gggattcaaa gaatattttt gcagaacgat tgaacactga tttgttctgg
     1501 aacggccgga attttattat tccctttttc tttctattag atattggtgt tttgtattgg
     1561 cgcttgattc gaagatatcg ccaaatgcaa ttgcggcata tcatcagcga acttcactac
     1621 atcgcaaacg gaaattatga tcatcgtatc ccttttgaac tgagtggtga cttaagccga
     1681 gtggtaacaa gtatcaatgg attggtggat agtaccgtag cagcgattga agacgaacgt
     1741 aaaatcgaaa aatcaaaaga tgaattgatc acgaatgtca gtcatgatat ccgtacccca
     1801 ctcacgtcca tcattggata cctaggactg atagaagatg gccagtatca ctcagaagaa
     1861 gatcttttaa agtatacgca tactgcttat atcaaagcaa agcagatgaa atctttagtg
     1921 gacgacttat ttgaatacac taaggtacgc cagcctgctg tcccggtgaa tttttctgcc
     1981 tttgacatga tccagttgat cgaacagtta gcagctgatt ttgagttgga agcttctaaa
     2041 aaaaatatcc aaatacttgt tcaatcaaaa gtagactccc ttatcatgga tggcgatacc
     2101 gaaaaacttg tacgtgtctt caataatctt ttgacgaatg cgttgaaata cggaaaagga
     2161 gcaacgaaga tcgtaatcga agtagaacgt atcggttcag aagttgttgc aacagtcaaa
     2221 aataacggag caatgatccc acaacaggca atcgataatc tattcgaccg tttttatcgt
     2281 gtagaagaat ctcgttcaca agcaactgga ggaactggac ttggtttagc aatcgctcaa
     2341 agcattgtgg ctttgcacgg cggatacatt tatgcaaaat cagataagca atggacttct
     2401 ttcatcatgc accttccaat caagaaaaat gaaaaattac ctattgatac acaggaagtt
     2461 attgacgaaa gctgaaatat tcgctattct tattacaatt attagaataa atgaaaaaca
     2521 cagaacccag tacactttat tctttttcag agagctgatg gcaggtgtaa atcagcaaag
     2581 gataatttgg aagttccagt tcgtttacgt gccctttcga aataagaaag gtcggttcaa
     2641 cagccgttat gtgttcaaca agtgcagctg acgctgaact tgggtggtac cgcgaatttc
     2701 agactgattc gtcccaagaa taatgggatg gatcagtctt tttttatttt caaaaaggag
     2761 gaaatcatat gttagatgta aaaatgatgc gacaaaatat tgaagaagta caagaaaaat
     2821 tgatgactcg gggcgtaaaa aaagaaacat tagctgaatt catggaacta gatgaaaatc
     2881 gtcgtaagct actagtcaaa agtgaagaat taaaaaaata tcgaaatgat gtttctgctg
     2941 agatcgccca attgaagcgt gcaaaagaag atgcttctgc taaaatcgct gaaatgaaag
     3001 aagtcggtgg caacatcaaa gcattggata ctgaaattgc tgaagtggat gaaaaattga
     3061 aacaaattgc aactactttg ccaaaccttc cccatgattc tgtgccagtt ggaacagatg
     3121 aagaggacaa tgtagaaatc cgtcgttgga gcgaaccacg tacatttgct tttgaaccaa
     3181 aaccacactg ggaaattgca gaaaatcttg atattttaga ttttgaacgt ggcgcaaaag
     3241 tagccggtag ccgttttgtc tattacaaag gattaggtgc tcgattagaa cgtgctgtct
     3301 acaacttcat gcttgatcag catatttacg aacacggcta tactgaaatg atcactcctt
     3361 atatcgtcaa cagtaaagcg atgtttggta cgggacaatt tccaaaattc aaagaagatg
     3421 ttttccaatt ggagaatact gatctaacat taatcccaac agctgaagta ccattaacta
     3481 actattataa tgatgaaatt ttagatggtg cagatttacc tatttatttc actgcactaa
     3541 gccctgcttt tcgttctgaa gccggaagtg ctggtcggga tacacgagga ctgattcgtc
     3601 ttcaccaatt caataaagta gaaatggtca aattttcgga tgtagagcat tcttacgaag
     3661 aattggaaaa aatgacagct gatgcagaag atatcttgaa aaaattgcat ttgccttatc
     3721 gtgtgatggc cttatcaact ggagacatgg gcttttctgc agctaaaact tatgacttag
     3781 aagtatggat acctgcacaa gatacttatc gtgaaatcag ttcttgttct aactgtgaag
     3841 atttccaagc acgccgcgca atgatccgtt atcgcgataa tgatggaaaa atccactata
     3901 ctcatactct gaatggttca ggacttgcag ttggccgtac agtagctgcc attttggaaa
     3961 actaccaaaa tgaagatggt tctgtcacag ttccagaagc gctcgttcct tatatgggga
     4021 atctgaaagt tattaaataa agaggctgtg aaaaagccgc ttagtttcga gtattaagaa
     4081 aaattttgtg aaaatggctt tccacatttt tgcataaatt ggacttaata ccgagaaact
     4141 ggcttttgaa caccatttat cgagaggttg tgaaatcagc gaattaacct gagtattaga
     4201 gaaaaaaatg gaaaaatagt ccctcatatt ttaccgtttg ttgaactaat accgagggtc
     4261 agctgattga acaccgttta gtggagaggt cgtaagccta ccccccagct cctagttaca
     4321 aggacaaatt caaaaaacag ctcttcatat tttatgaatg ttgtacttgt gattcggagg
     4381 tggcaaggga acactgttta ttcaaaaaga ggctgggaca gaagcgttta actccaagaa
     4441 ataagaagaa attcacgaaa attgcttttc aaatttttgt gaatttcttc ttatttccga
     4501 aggagttgct tctacttccg ccgtttatac gtgtttagag cgtgagacaa aagtgttctt
     4561 tacttttgtc ccacgctctt taatgttttt aagattcaac tgaatagttt ggtgcttctt
     4621 ttgtgatttg tacatcatga gggtgtgatt cttttaatcc actgccgctc atttggataa
     4681 attgcgcttc atcacgtaat tgctgtagat tggctgcccc aacataaccc attcctgaac
     4741 gcaaaccgcc gatcatttgg aaaataatat ctgcaacact tcctttataa gcaacccgac
     4801 cttcgattcc ttctggtact aatttatttg cttcattgac actaccttgg aagtaacggt
     4861 cttttgaacc tttttccatc gcgcctaatg agcccatacc tcggtatgtt ttgaatcgac
     4921 gtccttggaa gatttcgaat tcacctggtg attcatctgt ccctgccaac atacttccta
     4981 gcatcactgc atgtccgccg gcagctaatg ctttgacgat gtcaccagaa tatttgatcc
     5041 ctccgtctgc aataatcgct ttgccatatt gacgagcaac tgatgctgca tcataaatag
     5101 ctgtgagctg aggtacacct acaccggcta ccacacgtgt cgtacaaatc gatcctggtc
     5161 cgataccaac ttttacgaca tctacacctg catcatataa agcttttgtc gcttcagccg
     5221 tcgcaacgtt tcctgcaatc aatgttgcat ctgcaaaagt tgaacggatt tcttgaattt
     5281 ttcggatcac acctgcacta tgtccatgag ctgtatcgat gatgattgca tctgcccctg
     5341 cttctaataa agcattcgca cgctcaaatg tatcgcttgt tacaccaacg gctgctgcta
     5401 caagcaatcg accatgttca tcttttgctg catttggaaa ttcgatcact ttctcgatgt
     5461 ctttgatcgt gatcaatccg cttaaacgac cttcgttatc tacgattggt aatttttcga
     5521 ttttatgttt ttgcaaaatc ttttctgcat cttttagaga ggtacctact ggggcagtca
     5581 ctaagttgtc ttttgtcatc acatcagcaa tcggcatgtg ataatctgtt acaaaacgca
     5641 tgtcgcggtt tgtaattatc cctaccaatt ttctgttttc aagtgtttca acgattggca
     5701 caccactgat acgatatttg ctcatcaatt cttctgcgtc tgctacaaga tgctgtggcg
     5761 ttaagaagaa cggatcaatg attacgccac tttcagaacg tttgactttt cttacttcat
     5821 ccgcttgctg gctgatcgtc atgtttttat ggatcacacc tagtccgcct tgtcttgcca
     5881 tagcaatggc catcttgctg tcggtaacag tatccatact tgcactgatg attggaatat
     5941 tcaacttaat atttttagct aattgtacgc tcatatccac ttcgtttggt aacacgtgac
     6001 tttctgctgg tatcaataag acatcatcaa aagtaaatcc ttttttcgtg aactttgttt
     6061 cccagttgga catttttagt accactcctc taatttagtt tatctattaa aataattgat
     6121 tttttcattt aagtcaatga tctacttgga atcaagttag ctttctcata gaataccaat
     6181 ataaagcgtt tacatctgtg tgtattaaaa gagattgtaa aaaagctgtc ctgcatcaag
     6241 taataagaac agccaatcaa aaacagcttc tcatgttttt gaattggttg ggtttattac
     6301 cgcagatgca aacttttgaa caccgtttag tggagaggct gtgaaaaagc tgcttagttt
     6361 cgagtagtaa gaaaaatttt gtgaaaatgg ctttccacat ttttgcataa attggactta
     6421 ataccgagaa actggctttt gaacaccgtt tatcgtaaaa agggactgtg acaagacttt
     6481 tgtcacagtc ccttactatt atttaactat ggataaacaa aatgaggttc tttactaccc
     6541 ctagatatct agcaaaatag ctgatctttt tgctagttta tcttcttggg acggataaac
     6601 ttccaataat cccgtatttt ttcttcaagt agtaacggat tgctaaagca gttgccccga
     6661 taatgataac tactactgga tctaatactg gattgataac tggtggcaat agtgttgtcg
     6721 cagaaaacag tacgacccaa agaagtgtcg ttccacctag gataagcaat gtcttaccca
     6781 ttcctggttt cttgcttcga tctgctccgg gatattcata ttgatagatg tatttgtaca
     6841 tcatataaaa gacccagcca ccgattgcag aacctagaac gagtgtagaa aagccataag
     6901 ctggcgttgt tccacgtgaa aacaatccgg taatcgcaat catgatactt aatagaccaa
     6961 gcaacagcaa cgtattatct aaccacatca ataaaggggt ggatgaattt tttacttctg
     7021 ctggcttatt gatgattgct tccgttcttt cagagactgt cccaaacaat tgacgggctg
     7081 ttcttccgcc tttttgttct gcaactaaag taggcagaat gtcatgaagg gcttttactt
     7141 tttcttcttc agacaaattc gctgcttcta aagatttttt caaatcaaaa atgtactgtt
     7201 gatttctttt ggttaatttt gtttctaaac tattgttttc ttttacgatt gctcgtaatt
     7261 gttctggttc cattatctaa tagtcctcct tagccgttcc ttttctcaac ggtcgtcaca
     7321 aaagacattc taccatattc tacagctagt ttgatagccc ttttacacat taaagcggaa
     7381 taacattacg tcgccgtctt gtacgacata atcttttcct tctaatcgaa cacgtccagc
     7441 ttcttttgct gcatgcatgt ttccgtaatg atccaagtct tcaaaagaga cagtttctgc
     7501 acggataaat ccgcgttcga aatctgaatg gatgattcct gctgcttgtg gtgctttgat
     7561 tccttttttg aatgtccaag cacgtacttc ttgttcccca gcagtaaagt aagtagctaa
     7621 gcctaacaaa tcataagcag cacggatcaa ctgatccaaa cctgattctt cgattcctaa
     7681 agcttctaaa aattccgctt tgtcttcttc atctaattct gcgatttctt cttctgcacg
     7741 agcactgacc acgatcactt ctgcattttc ttctgcagca aagttgcgca cttcttgtac
     7801 ataatgattc gcgtctgcat ctgctacgtc atcttctgag acattggcta cataaagaat
     7861 tggttttgtc gtcaataaaa atagtgattt aacgattttt tgttcttctt cagtaaattc
     7921 gatcgttcgg gcagattttc cttcttccag tacaggtttg attttatcca gtacagctag
     7981 ttctgccact gcatctttat ctttggtttt agcaactttt gctacacgag tatagcgttt
     8041 gttgatagat tctaagtctg ctaacactag ttccaagttg atcgtatcaa tatcttctag
     8101 tggatcaaca cgtccttcta cgtgagtaat attatcatca tcaaaacagc gaacaacgtg
     8161 acaaatagcg tctacttggc ggatatggct taagaattga tttcccaaac cttctccttt
     8221 acttgcgcct tttacgattc cagcaatatc tgtaaattca aaagtcgtcg gaacagtttt
     8281 tttcggatgt acaagttctg ttaagcgttg caaacgccag tcaggtactt ctaccatccc
     8341 tacgttgggg tcgatcgtcg caaagggata gtttgctgct tctgctcctg cttttgtgat
     8401 cgcattaaac aaagttgatt ttcctacgtt gggcaaccca acaattccag cagtcaatgc
     8461 cataaattta ttcactcttt ctctgcattt ttttcaatta ctttttttaa tttcttttca
     8521 aaatctcgac gggtcatcat gacgatatgt ccgcaattca tacatctgat tttgatatct
     8581 gcgcccattc gaatgacttc ccagcgattc gtctggcaag cgtgaggttt tttcatttca
     8641 acgatatcgt gcaagtcgta cataaaaata gcctcctttt tgtttattct tcatcaaatt
     8701 gaatattcaa gatatcaaga attcgagtca aatctttttg agaaagatat tcaatctcga
     8761 tcttaccttt cccatttttt tcttggatag ctacacttgt accaaattta tccatcaaac
     8821 gatcttcgct ttctcggata taatatggtt tttcctttgc tagccttggg atttttttct
     8881 tttcttttcc atcattttga tttaactcag aaacaagctg ttccagttgg cggacagtta
     8941 aattttcagt tactgcacga ttggctaatt tcaagatcaa tgttttattt tttaaaccta
     9001 acagcgtacg ggcttgtccc atagataaac gctgatcttg taccatttcc ttgacaagat
     9061 ccggtaaaga aagcaagcgc aaataattgg cgatataagg tctgctcttt cctaaacgac
     9121 tagctacttc tgcttgtgtt agtttcaggt ttttcatcag catctcatat gcttctgctt
     9181 cttctaacgg attcagatct tctcgctgca agttttccag cactgctact tgcatcatcg
     9241 cttcttcgtc aaagtctcta acgatagcag ggatcgtttc tttttcagct agtttggacg
     9301 ctcgaaaacg tcgctcccct gcaataatct catatccttt tactgcggaa cggcgtacga
     9361 tgatgggttg aaagacacct gattgttcaa tagaattagc tagttcctgc aaagatactt
     9421 cttcaaatgt tttacggggc tgatacggat ttggtcgcag ctcattcaat ggtagctgga
     9481 caacggtatc atcttttaca tccaccgtct ctaagttttc taaatcttga aacagtgcat
     9541 cgattcctcg gcctaatcct ttacttttct tcacgactta ccacttcctt tgccaatgct
     9601 tggtagactt cagctcctcg agaacgtgga tcgtagtcga tgatcggttt tccatgacta
     9661 ggtgcttctg ataaacgaat atttcttgga atgatcgttt cgtaaacttt ttcgcggaag
     9721 tatttacgta cttcttctac gacttcattt cccagatttg ttcgtgcatc atacattgtc
     9781 agtaagactc cctcgatctc taattctgga ttaaagtgtt tttggactaa acgaaccgta
     9841 ttcagtaatt ggcttaatcc ttccaatgcg taatactcac actgaacagg tatcaaaatc
     9901 gaatcacttg ctgtaaatga attgattgtc agatggccaa gagaaggcgg gcaatcgatc
     9961 aaaatataat catactgact gcttacttct gctaaagatc cttttaatct tgattctctt
    10021 gccatcattg acgtcaattc gatctctgct cctgctaatt ggagagtcgc aggcacaata
    10081 ctcaaatttt cgtgttcagt gatcaaagtt gcctcatcaa ttggcaattc attgactaga
    10141 acatcataga tgtctcttgt cacatcaggt tttctgatcc cgacaccact ggtagcattt
    10201 ccttgtgcat ccatatccac tagcaatact tttttgccaa ggctagcaag acaagcacct
    10261 agattcaccg tcgtcgttgt tttgccgacg ccaccttttt ggttcgcaac agatattatt
    10321 tgtgccatct cttctcctcc tatttgattg gctgcttatt aggcagtccg ggctttcttg
    10381 ggtatttctt tggggttggt ttcttttttt gaataacgat gatatgccgt tgatcagaga
    10441 cattcggcaa ggtaaccgcc tcttctttga ggaatttacc acctaataaa gcaatcgctt
    10501 gttttgcttc tgtcaattcc tcttcacttt ttgctgcttt caatgcataa aaatagcctt
    10561 cttgtttagc caagggcaaa cacaattccg ctaacacatt caaacgagca accgcacgag
    10621 cagtgacaag gtcaaatgat gcacgaaagt gaggattttg cccaaatgtt tctgcccggt
    10681 catgatataa ggaagcatcg attcctaata atcgggttaa ctcagataag aaggttattc
    10741 gtttattcag agaatcaacg atcgttatct gcagttctgg aaaggcaatt tttaaaggta
    10801 tgctaggaaa ccctgccccc gaaccaacat cgcataaaga taaaggttgc gtaaagtcta
    10861 cttcaaatgc taatgtaagt gaatcgtaaa aatgcttcag atatacgtct ttttcatccg
    10921 taatagctgt aaggttcatc ttttcgttcc attcgactaa tgtccgataa taaatagcga
    10981 attgattcat ttgatgatcg gtaagctcta tccctttttc tgccaaggat tggcgaaatt
    11041 cttctggtat cataaatatt cctttcattg tgaaacagca attgtttcac agattctctc
    11101 cctactttaa cgcaaaatct ctagacatgc aatccttcca tatggattct taaaaatttg
    11161 aaaagtaaga aaagccgata gactttttct ttctagtatg actcataaaa tatacacaga
    11221 agagagaact agctttgagc aatttctgca aaaactagtt ctctcttcta tttattatta
    11281 cgtattacgc tggttcactg actttcgcaa tcttcccttg ttcgatatag accattagga
    11341 tacttaggtc tgcagggttt acgccactga ttcggctagc ttgggcaatt gtttctggtt
    11401 ggattttctg caatttttgt tttgcttctg ttgctaatcc gttgattgct gcgtagtcga
    11461 tgttttctgg aatctttttc gcttccatgc gttttaattt ttctactttt tccatggctt
    11521 ttttgatgta gccttcatat ttgatttgga tttctacttg ttcgatttct tttcgtgtca
    11581 attcttcatt ttctggaata aaccgtagaa gttctgcata agtaatctca ggacgtctta
    11641 aaaagtcgct tgctagtaca ccatctttta atggtgccgc attaatcgtt gctaaaaagt
    11701 cttggacttc ttctgttggt ttgattcgta cattcatcaa acgtttgatt tcttgttcaa
    11761 ctgcttcttt tttcttcaaa taattcttgt attgatcttc ttttactaag cctacacggt
    11821 gacccatttc tgttaatcgc aaatctgcat tatcatgacg taaaagcaga cggtattcag
    11881 cacgtgaagt cagcaaacga taaggttcgt tcgttccttt tgttaccaaa tcatcgatca
    11941 tgacaccgat gtaaccgtca cttcgtttga tcactaaagg ttcttttccc tgaactttca
    12001 aagctgcatt gataccggca atcagccctt gtccagccgc ttcttcataa ccgcttgtac
    12061 catttgtttg tccagctgta taaagatttt caacgacttt agtttctaat gtcggacgga
    12121 gctggtgtgg ctctactacg tcatattcga tagcatatcc tgtacgcatc atttctactt
    12181 tttctaatcc agcaatagaa tgaagcatgt tgacttgaac atcctctggt aaagaagtag
    12241 ataaaccttg aacatagact tcttccgtat ttaagccttc tggttccaaa aataattgat
    12301 gacgtggttt gtctgcaaaa cgcacgatct tatcttcgat ggatggacaa tagcgagcgc
    12361 ctaccccttc aacgatccct gtaaacattg gggcacgatg taagttgtct cgaataattt
    12421 ggtgagtcgt ctcacctgta taggtcagcc agcaagattc ctgatctagt ttataggcac
    12481 tatctggtgt gctgaagcta aaatgattcg gctctttgtc accaggctgc tcttctgtaa
    12541 cagaatagtc gatcgtactt gattttactc gaggcggtgt tcctgttttg aaccgttcga
    12601 tagcaaagcc aagttctttt agatgattcg ctagtccaat agaaggttga gagttattcg
    12661 gacctgatga atatttcaat tcaccgataa tgatttctcc gcgaagtgct gttccggctg
    12721 taataacaac tgctttggcc cggtagctcg ctcctgtgga cgtcactact ccatgacaaa
    12781 caccatcttc gacgatcagt tcttcgacga ttccttgtct tagcgtcaaa tttggttctt
    12841 tttcgatcgt atgtttcatt tctgttgaat aagcatgttt gtctgcttgt gctcgcaatg
    12901 ctcggacagc tgggcctttt cctgtgttca acatacgcat ttggatatag gttttatcga
    12961 tattcttacc catttctccg cctaacgcat cgatttctcg aacgacaacg cctttagctg
    13021 gtccgcctac agaaggatta catggcatga atgctaccat gtccaaattt attgtcagta
    13081 ataatgtttt tgatcccata cgtgcagcag caagtgcagc ttctgaaccg gcgtgtcctg
    13141 cgccaacaac gatcacgtca tacgcatctg cttcataatg ttccaatcgt ttgttcctcc
    13201 ttttattttc ctaaacaaaa ttgactgaat aattgagtga ttagctcatc ttgtacactg
    13261 tcgccgacaa cttctcctag atagtcccag caacgcgtca tatctatttg aacgagatcc
    13321 acaggcattc ctgaatcaat acctgcaatg acttcttcaa gtgagaggga agctttttct
    13381 aacaacgcga tatggcgggt attagaaaca taagtagcgt ctctttctcc tgtttctccg
    13441 ctgaagaata aatcagaaat agctgattcc aaacgatcta gtccgtcgtt tttagctacg
    13501 gaaattgaaa aaactggttc attttcgatc aatttcttca atttttcttg ttccagctgt
    13561 gctggcaaat ctgttttatt tagaagaatg atccgtttca atccacttgt cgcttccaat
    13621 aattgttcat cttctgctgt cagtggttcg ctttggttta gaacaagtaa gatcaagtct
    13681 gcttctgcta atgcttttcg gcttctttcg accccgatcc gttccaccac atcttctgtt
    13741 tcccgaattc cagctgtatc gattagcttg agcggtaccc cccgaacatt gacatactct
    13801 tcgattacat ctcgagtcgt tcctgcaata tccgtaacga ttgctttttc ttctcttaac
    13861 aaatggttta acaaacttga tttgccgaca tttggccggc cgatgattgc cgtacttaac
    13921 ccttcccgca gaaccttccc ttgcttggat gtagcaagta gcgcttggat tctttgttgg
    13981 atcatctgcg ctttttcaag caataacttc gtagtcaatt cttcgacatc atcatattct
    14041 ggataatcaa tatttacttc tacttgtgct aatgtctcta aaatctcttg tctcaatgag
    14101 cggatcaaag cagacagatt gccgtctaac tgattaagag ctagtcccat tgctttgtct
    14161 gtttttgccc gaatcaaatc catcacagct tctgcctgtg aaaggtcaac acgaccattc
    14221 aagaatgctc gttttgtgaa ttcgcctggc tcagctaatc ttgcaccttc tcttaacaat
    14281 agctgcaaaa tttgattgac tacaacgatc ccaccgtgac aattgatttc tactacatct
    14341 tcccgcgtaa atgtctttgg tgctctcatc acggagacca tgacttcatc gacaagctga
    14401 ttgctcttgg gatcaacgat atgtccataa tggatcgtat gacttggtac ttctgataat
    14461 cgtttgtttc cagattgata aactttatct gctagttgca cagcttgatc gccacttaaa
    14521 cgaacgatac tgattgcacc ttctccaggt ggggtagaaa ttgccgcaat cgtatcaaat
    14581 tctaaagtaa tttcagccat tttttcactt cctttttcat tttgcgcaca aaaaaagtgc
    14641 ccactccacc ctttatgcac aataaacggt gaacttcgca ctttgactgc ttgttcttga
    14701 ttaaattctg gtcatagatt atcacacttg ctataaggaa tcaagtgttt aaaacaatta
    14761 gatgaagttg agcctgttct gcttccatta ccttatattt aattgtcctt ggatgtctct
    14821 ttgcgttctt tggtttattc gaagagaaga tactgtataa acaaggcttt atctttctta
    14881 aaaaaaacgg tacactaaat ggtagcgcta tctaaactat acacttttga aaggagaaaa
    14941 tatgtgagcc cgttcagatc tgctgcattg gaagaagaaa gtgaccctcg gatcaacgat
    15001 gtcgttttat ctacagggaa tatcaaccag aaatatatcg tccgcgatat cgtttttgtt
    15061 gtggaatgct tagaagctga tttgtttgac ccatcttttc ccaaagatga ccttttctta
    15121 acaacgaaga ggaaattaaa gacgaaggta cttgaatatg gtgcgaatgc agtaatcaac
    15181 tgtcattttg agtatcagca aaaaaaacgc gacggaaagt attttctgga actttttgct
    15241 tatggaacag tgatccaatt tatccaaaca acgattggtt aaatgaaaca gacaaaaaaa
    15301 gcggatcaaa actgaagaca gtttcgatcc gcttttttct atcaaagtta gtgactactt
    15361 catcgtatac tctgataaga atgatttcta gtaaaatata cttggtttga aaaactttgt
    15421 caacgtgatt tattcaaaaa gtaatggaat caataatatt ttttagcagg ttctactact
    15481 aaataacgat aaggttcctc accttccgaa tgtgttttta catgttcatc tttgcttaat
    15541 gcagcatgga tttgtttacg ttcaaatgca ggcattggtt ctaagaaaac aggctgccct
    15601 gtccgtttta ctttttcagc cgtgcgatca gctaaacgct gaatgatctc ttggcgcttt
    15661 tcacggtaat tgcctacatt gactactaca gacagcttat ttcttgctaa acgatggatg
    15721 aagacttgtg ccagatattg caatgcattc agtgtttttc catgtttccc aatcagtatg
    15781 ccttgctttt gtgtttctaa atggaaaaca accatattat ttgattctct ttccattttg
    15841 acaagagctg gagcatcaag ctgttttgaa atttcagtaa gataaactgc caattccgtt
    15901 aaagctgctt catcacctag ctcacttact tcttctgttt tttcttcaag aatcgtttcg
    15961 ttgactacct cgatctcagc tgttggttct tcttccaaaa tttcttctac agtctgttct
    16021 actgtttcag aaactttttc gctgatactt ggctggatcg aaagccgtgc attctttcga
    16081 cccattccta ggaaaccttt ttttccttct tccagtattt cgatttcgac ttggtctttg
    16141 gccaaaccca aatcagccag acctagttgg acagcttcgt ctattgttgc agcttcataa
    16201 atcggcatta gcattacctc ccctattatt tccgtttttt cttaggattc attgctttct
    16261 taagagcacg ttctctttcg cggattctcc gtgcttcttc ttcacgctct tttcgaattt
    16321 tgaaagggtt gttgatcagc agcgtttgta caacttggaa ggcattggaa actacccagt
    16381 ataatgaaag accgctggct aaattgacac ccatcactaa aatcattaca ggcatcacga
    16441 aattcatgat cttcattgat gcattcgatt caatttgact catgctagat aaatacgtac
    16501 ttgcaaaagt aaagattgct gctaaaatcg gcaaaataaa tgttggatcc gcgttcccta
    16561 agttcaacca taagaaatgc ccttgtttca actctggtac acgtgaaata gattgccata
    16621 acgccatcaa gattggcatt tgtactaata aaggaagaca accagcatat ggattgacgc
    16681 cattttctgc atataaacgc tgctgctctt ctcttaactt actttgtgtt tccggatctc
    16741 ttgaactata ttgttgctgc agtgctttta atttcggttg taattcttgc gttttacgca
    16801 tgctctttgt ttggaaatgc atcaacggca ataggatcac gcggatgatc agtgtaaata
    16861 aaatgatccc tatccctgca ttaccaaatg acaacgcctt gatcgcctca gcaaagtaat
    16921 aaacaatata acgatcccaa attcctgtac tctgtgcgct gacttcccca gttccgcaac
    16981 cggataagac gatgaccaaa ctaacgatac cggccatcaa aagtaaacgt ttgtatttct
    17041 tcacttactc ttttcctctc tgtcgattat ttttgcaagt tttaagacgt ggactaaatt
    17101 ggagcgaacc tcatcataag tcaacccctt cacacttggt ctagcaatca caataaaatc
    17161 caactccgga tcaatttctt cttttataga ataaactgct gctcggattt ttcgtttgac
    17221 ttcattgcgg tttaccgcgt tgccgacctt ttttcctaca gataaaccaa ctcgaaaatg
    17281 aggctgtccc ggtttttcca aacggtaaac gacgaatttg cggttagcaa aagaatctcc
    17341 cgtatggaac acggcttgaa actctcgttc tttttttact cgatacgctt ttctcattag
    17401 ttttctttcc ttttctatct ctcacttacg ctcataatca taccataatt acgattgtcc
    17461 tttcatcatt tccctaaaaa agagtataaa aaaaaccact gaaaccccag tggcctaagc
    17521 tgaaagaact tttcttcctt tgcgacgacg gctagctaac acacgacgtc catttttagt
    17581 actcatacgt ttacggaatc cgtgaacttt ttgacgttta cgtttatttg gttggtatgt
    17641 tcttttcata tattccacct ccaaaatgtt cttatctatt ttataaacac agacatactt
    17701 tgatagtata acgacaaagt agtgattttg tcaatataaa atagaagatc gattgttaaa
    17761 acttatccac aagattattt ttttctatgg gaatcctatg tggaaaaagt tatgcacagg
    17821 gattatccac agataataaa aggctggttt tacacagaaa acacacgtgt aaaaaaagta
    17881 atccacaatc aggcgatttc ctgtgtattc cgtctcctaa ctattggtat atctgtattt
    17941 tttatccaca gacgcaagag gaaatctatt ggtttttacg agttttccac ctgtgcatta
    18001 gtggtgtgga aaactttttt tgacaactgt gtttttctct tttttctttt tgtcgattgt
    18061 gtggataatt gtaattaaaa aatttttttc ttcggtttct gtggaaaact attgaaaaaa
    18121 atgctaaatt agtactagtt attcactttt taatgaagga ggaggcctta tatggtatcc
    18181 ctcgatgctt tatggaatga attaaaagca acataccaaa aggatttatc gcctgctagc
    18241 tataatacat ggatcgaaac agcgaaccct cgaacccttg atcaaaacca gctcgttgtt
    18301 gaggttccta gcaagattca taaagaatat tgggaaaaaa acctggcgac aaaaatcgtt
    18361 gaagtcggtt atatgttatc aggaaatgag attatccctc gctttattac tggcgaagaa
    18421 gcagagcaag aagaagttat agaagaaaaa aatccaaaag ttgtggcacc tagtccgcta
    18481 aaaaaagcca tgctgaaccc taaatatacc tttgatacgt ttgtcattgg taaaggaaac
    18541 cagatggctc atgcagcagc gctcgttgta gcagaagatc caggttctat ttataacccg
    18601 ctcttctttt atggaggcgt gggacttggt aagactcact tgatgcatgc aattggtcat
    18661 caaatgctgc aaagtcagcc taatgccaaa gtaaaatatg taagcagtga aacttttgct
    18721 aatgatttca tcaattcgat ccaaaacaaa acagcagaag agttccgtca agaatatcga
    18781 aatgttgatt tactgctagt ggatgacatt caattttttg cagaaaaaga agctacgcaa
    18841 gaagagtttt tccatacttt caatgccttg tacaatgaag ggaaacaaat cgtactaacc
    18901 agtgatcggt tgccaaacga gattccgaaa ctgcaagaac gccttgtttc tcgctttgca
    18961 tgggggctgt ctgttgatat tacaccgccg gatcttgaaa caagaacagc gattttacgt
    19021 aaaaaagcgg cggctgaacg tctagagatc ccagatgaca cactaagcta catcgcagga
    19081 caaattgatt ctaacattcg tgaattagaa ggtgcgcttg ttcgagtgca agcttttgct
    19141 gcgatgaata gtgaagatat ttcgactagt ctagctgcag acgcactgaa aacgttaaaa
    19201 tcaggaaaag gacatcctca attgtcaatc ctgcagatcc aagaagaagt ggcaaagtat
    19261 tatcacatcc aattgaaaga tctcaaagga aagaaacgcg tcaaatctat tgttgttcct
    19321 agacaaatcg ctatgtattt ggcacgtgag ctcacagata attctttacc gaaaatcgga
    19381 gctgaattcg gtggcaagga tcatacaacg gttatccatg ctcatgaaaa aatccagcag
    19441 ctgatggatt ctagtgtcag tatgcaaaat gaagtatcag aaatcaaaaa ctttttacta
    19501 aattaatttg tcgattctat caactgaaaa tagcatgtgg ataaaagaaa aaaacgttca
    19561 aaagttatac acactttttc cacagctgtt ttttcgtttc tctcttacgt tttttgagtt
    19621 ttccacagaa tcaacatgcc ctattacttt tattattctt tttaactaat aataaataaa
    19681 caaacattca aaggagatac atcatgaaag ttactttaaa ccgagctagc tttatgcagg
    19741 aattgcaaac tgttcaacga gctatttcaa gcaaaaccac gatccctatt ttgacaggtg
    19801 taaaaatcac actgacacaa gaaggtttga ctttgacggg gagcaacgct gatatatcaa
    19861 ttgaaacttt tttgtctgtt gaaaacgaaa aagcaaatat gcaaatcgaa tctactggtt
    19921 ccattgtttt acaagcacgt ttctttagcg aaatcattcg gagacttcct gaagaaacat
    19981 ttactttaga agttttagaa aataaacaag tagcgatcac ttctggaaaa gcgaatttta
    20041 tcgtaaatgg attagatgca gataactatc ctcatcttcc tgttgtcgaa agccataacc
    20101 agatgaaatt acctgtacac gtattgacta aactaatcaa cgaaacagtt tttgctgtct
    20161 cacaacatga gagtcgtcca atcttgacag gtgtgcattt cattttatct gataattctt
    20221 tattagctgt agctactgat tctcaccgtc taagtcaacg cgtgattcca gtagaacaag
    20281 cggctgatca ttttgatatt gttattcctg gaaaaagttt gatcgaatta tctcgctcat
    20341 taacaaatga agaagaaatc gtcgaaatca gcattatgga aaaccaagtg ttgttcaaaa
    20401 cagaaacgat gtatttctat tcccgtttgc tagaaggaaa ctatccagat accaatcgtt
    20461 tgattccttc aagttttaat acagaggttg aattttctgt tcctagcttt ttagcggcga
    20521 tcgaacgggc ttctttactt tctcatgaag gccgtaacaa catcgttcgt ttatctattc
    20581 gtccagatgc ggttgtttta tacggaaatt cgccagaaat cgggaaagta gaagaaagct
    20641 tgagttatac agcaagcagt ggcgatccat tagatatttc tttcaatccg gattatatga
    20701 aagcagcgtt gcgtgcattt ggagatatga gcatcaaagt gaaatttatc tctgctattc
    20761 gtccatttac attggaacca acggaagatg gcgttcaatt tattcagctg attactcctg
    20821 tacgtacaaa ttaacaatga ttttcaatgt tcttgaaaat ttaattgaaa agaaatagaa
    20881 aaaataaaat tttaaacaga aggccctaaa atctctttta aggctttctg tttatttttg
    20941 tttaaatcac cttttttaga ttaaatcgtc ttatatggaa aaatatatgg ataaatttgt
    21001 tttattgcga aaaaaaaggt ataatagagt atacttttaa taatgaaaat gagggatggg
    21061 cattgaaaca acaagtcatt ctaaatacgg aattcatgac gttgggccaa atgttgaaag
    21121 aagtcacagt tatcggtagt ggcggacaag caaaatggta tttggcagaa aacactgtgt
    21181 tagtcgatgg cgaaccagaa aatcgtcgtg gacgcaaact ttatcccggc atgatggttg
    21241 aagttccaga agtaggaact ttttttatgg taaaaaaagg aaacgacgat aatgaggctg
    21301 aataagctgt atttaaaaaa ttatcgaaat tacgaagagc tgaacatcga attttccaag
    21361 aacctcgtca tctttttagg agaaaatgcg caaggaaaaa cgaatctttt ggaaagtatc
    21421 tacgtattag ctatgacgcg aagtcatcgg acaagtagtg agcaggaatt gattggctgg
    21481 actgacgatc aggcgatgat tcaaggagag atcacaaaag gttcatccac tcttccatta
    21541 gaaatcttat tatctaaaaa aggccgaaaa accaagatca accatatcga acagaaaaag
    21601 ttaagtactt acgtgggaca aatgaatgtc attttatttg caccagaaga tctttcttta
    21661 gtaaaaggca gcccgcagat caggcggaag tttttagata tggaattagg ccagatcaat
    21721 ccaatctatc tttatgatct tgtccaatac caagcaattt tgaaacaaag aaaccagtac
    21781 ttgaagcaat tgaatgaaaa aaaacaaact gatttgcttt atttggatat tttgtccgaa
    21841 caactagcag cgtttggcag caaagtattg aaagcaagag cacagttcgt caagcgattg
    21901 gagttttggg ctaattcttt gcatcagcaa atcacccatc aaaaagagca gctggaaatc
    21961 gagtatctca ccgcagtcga ttctttagaa acacatacgc aagagcaaat ccaagaacaa
    22021 tttctcgcac ttttgaacca aaataaaaag aaagaccttt ttcggggaac aacgacagtt
    22081 ggtcctcatc gtgacgatct ttcctttttt attaatcaga aaaatgtgca gacttatgga
    22141 tcgcaaggac agcaaagaac aactgcacta agtgtcaaat tagcagaaat cgatttgatt
    22201 aaagaagaga caggggaata tccaatcctt ttgttagatg atgtgatgag cgagctggat
    22261 gataatcgtc aactccatct gctagaaaca atcgaaggaa aagttcaaac gttcttaaca
    22321 acaacgacac ttgaccatgt aaaaaataaa atgactgttg aacctgagat tttttatgtt
    22381 caacaaggga aactaaaagg agaagaaact gaatgacaga agaaagaagt ttagtagaac
    22441 gcgctaaaga gtatgatgcc agtcagattc aagtacttga aggacttgaa gccgttcgta
    22501 aacgacctgg catgtatatc ggttcgacta gttctgaggg tctgcatcac cttgtttggg
    22561 aaattgtcga taattcgatc gatgaagtat tagcaggttt tgcaacgaaa atccatgtga
    22621 ttattgaaaa agacaacagt atcaccgtta ttgacgacgg tcgggggata cctgtagata
    22681 tccaggcaaa aactggacgt cctgcagttg aaacggtctt tactgtttta catgctggag
    22741 gaaaattcgg cggtggtggg tacaaagttt ctggtggact tcacggagta ggatcttctg
    22801 ttgtaaacgc gctttctaca cagcttgatg tcaaagtata caaagaagga aatgtgtact
    22861 atcaagaata tcgtcgtgga gctgtagtgg acgacttaaa gattatcgaa caaacggatc
    22921 gccatggaac aacggttcac tttacgccag accctgaaat ttttacagag acaactgagt
    22981 ttgatttttc aaaattggct acccggatcc gtgaacttgc tttcttgaac cgcgggatgc
    23041 gtatatcgat tgaagacaaa cgagaagaag aaccagtcat caatgaatac cattatgacg
    23101 gcgggatcaa aagttatgtc gaatacttga atgcaaacaa aaccgtgatt tttccagaac
    23161 ctgtatattt ggaaggtgaa cagcaagata tcgcagttga agtctctatg caatatacag
    23221 acggctacca ttccaatatc atgagttttg ccaacaatat ccatacgtat gaaggtggaa
    23281 cacatgaatc aggattcaag actgctttaa ctcgtgtgat aaatgattat gcccgaaaac
    23341 aaaagctgat gaaagaaaat gacgacaact tgaccggtga agacgtgcga gaaggattga
    23401 cagctgttat ttctatcaag catcctgacc ctcaattcga aggacaaaca aaaacaaaat
    23461 tgggcaattc cgaagtacgg acagtcactg atcggttatt tagcgaacat ttcatgaaat
    23521 tcttgctgga aaacccaagt gtgggccggc aaattgttga aaaaggatta ctagcttcca
    23581 gagctcgtct agcagcgaaa cgtgcgcgtg aagtaacgag acgtaaaggt gctttagaga
    23641 ttagtaatct gccaggaaaa ttagcggact gctctagcaa cgatcctgaa aaatgcgaat
    23701 tgttcatcgt cgaaggagat tcggccggcg gatcagctaa gcaaggtcgt aatcgagagt
    23761 ttcaagccat cttgcctatt cgtgggaaaa tcttgaatgt tgaaaaagca agcatggaca
    23821 agatcttggc aaatgaagag atccggtctt tatttactgc tatgggtaca gggtttggag
    23881 aagattttga tgtatcgaaa gctcgctacc acaaattagt tatcatgact gatgccgatg
    23941 tcgacggcgc gcatatccgt acattgctgc tgactctttt ttatcgttac atgcgtccta
    24001 ttgttgaagc tggatacgtc tatattgctc agccaccttt atacggggta aaacaaggga
    24061 agaatattac gtatgtacaa ccaggaaaaa atgcagaaga agagttgaaa caagtcgttg
    24121 cttcacttcc tgcctcacca aaaccaagcg ttcagcgtta taaaggactt ggagaaatgg
    24181 atgatcatca actatgggaa acaactatgg acccaagcaa tcggatgatg gcccgcgtca
    24241 gtgtggacga tgctattgca gcagatcaga tatttgaaat gttgatgggg gatcgagtag
    24301 aaccccgtcg tgcattcatc gaggaaaatg cacactatgt gaagaactta gatatttaat
    24361 catgaaggag gaaaaagaat gagtgaagaa atcagagaaa acatccatga tgtcaatcta
    24421 accagcgaaa tgaaagactc cttcatcgat tatgcgatga gtgttattgt cgcacgtgca
    24481 cttcctgatg tcagagatgg tctgaaaccc gttcatcgcc gtattttata cggcatgaac
    24541 gaattaggtg taacaccgga taaagcccat aaaaaatctg ccagaatcgt cggagatgtc
    24601 atgggtaaat accatcctca tggagacatt gcgatctatg agtcaatggt acggatggca
    24661 cagccattca gctaccgtta tatgctagtg gatggtcatg gaaactttgg ttctgttgac
    24721 ggtgacggtg ccgccgctat gcgttatacc gaagcacgga tgagcaaaat cgctacagaa
    24781 atgctgcgtg atatcaataa gaacactgtt gatttccaaa gcaactatga tgatacagaa
    24841 aaagaaccag ttgtactacc tgctcgcttc ccgaatcttt tagtgaacgg aacaacgggg
    24901 atcgccgtag gtatggccac gaatatccca cctcataact tgtctgaggt agtagcggct
    24961 atcgatttgc tgatggataa tcctgatgtg acgaccaatg aattgatgga agtattacct
    25021 ggaccagatt ttccaacagg tggtttggtt atggggaaat caggaatccg aagagcgtat
    25081 gaaacaggta agggttctat taccgtacgt gcaaaagttg agattactga gatgccaaat
    25141 ggtaaagaac ggatcatcgt gacagaattg ccttatatgg tcaataaagc aaaattgatc
    25201 gaacggattt ctgaactgca cagagacaag cgtatcgaag ggatcacaga tttgcgggac
    25261 gaatcttccc gtgaaggtat gcggatcgtc attgacatcc gtcgtgacgc cagcgcatcc
    25321 gttatcttga ataacttata taaattgaca tccttgcaaa attcatttgg attcaatatg
    25381 ttggcgatcg aaaaaggtgt gccgaaagtt ttgagcttga aacaaatcct tgaaaactat
    25441 atcgaacatc aacgcgaagt catcacacga cggacggtct ttgaaaaaga aaaagcagaa
    25501 gcaagagcac acatcttaga aggtttgcgt attgctttgg atcatatcga tgaaatcatt
    25561 gcgattatcc gcgggtctaa atcagatgac gaggcaaaag caacaatgat cgaacggttc
    25621 gatttatccg atcgccagtc tcaagctatt ttagatatgc gtttacgccg attgaccggt
    25681 ttggaacggg aaaaaatcga aaatgaatac caagaactct taaaactgat tgaagattta
    25741 actgacattc tggcaagacc agaacgtgtt acagaaatca tcaagaatga attagcagac
    25801 ttgaatcaac gatttggtga taaacgacgc acagaattac tagtcggtga ggtcttaagt
    25861 ctggaagatg aagatttaat cgaagaagaa gaaatcgtta ttacattgac aaacaatggt
    25921 tacatcaaac gtttggcaaa taatgaattc agagctcaac gaaggggtgg acgtggtgtc
    25981 caaggaatgg gtatccacaa tgacgacttc gtcaaaaatc tggtttcttg ttctacccat
    26041 gatacattgt tattctttac gaataacgga aaagtgtatc gggcaaaagg atacgaaatt
    26101 ccagaatacg ggcgtacagc caaaggaatc ccaatcatca atttactagg tatcgattcg
    26161 agtgaaagca tccaggcaat cattgcggtg acgggagaag cagaagaagg ccactatctc
    26221 ttctttacga cgcgtcaagg tacagtaaaa cgtacttctg tcactgcttt tgctaacatc
    26281 cgaagtaatg gtctgatcgc aattggtttg aaagaagacg atgagctcgt caatgtgcta
    26341 ctgacagatg gtcaatctaa tatcattatt ggaacacata acggctactc tgtcactttc
    26401 aaagaagaag cagtccgcga tatgggacgt acagcttccg gggtcagagg gatacgcctg
    26461 cgtgaggaag attatgtcgt tggggcagcc ttaatgaagg aaaatcaaga agtattagtg
    26521 atcacagaaa aaggttacgg aaaacggaca aaagtatcgg aatatccagt gaaaggccgc
    26581 ggaggtaaag ggatcaaaac ggcgaacatc actgaaaaaa atggtccact agctggattg
    26641 accactgttt ctggagatga agacatcatg ctgattacag ataaaggtgt cattatccgt
    26701 ttcaacgtat cgactgtttc tcaaactgga cgttcaacat taggcgtacg tttgatgaag
    26761 atggaagctg atacgaaagt tgtcacgatg gcagcagtcg aaccagaagc tgcagaacaa
    26821 acagttgaag aacaaggatc agaagaataa ccatcaagac taagtgtgta agctagcaag
    26881 aataaccgat taaaaaatct ggttattcct gctggctttt ttgattagtt gaatttctct
    26941 taaaatattc gaaactgttc attctgtatt gcttttgacc aaaatagagt gtataattag
    27001 agcttgtgag tagtctaatt aattagataa ctctccttgc tcttaggaac ctaagagccg
    27061 aaagtccaca gggaggtgaa tatcaatgga aaacacgaaa tatgaaatca tgtacattat
    27121 tcgtccaaac attgatgaag aagcaaaaac tgctttaatc gaacgtttcg atgcaatctt
    27181 gaaagataac ggagcagaag ttatcgaatc aaaagattgg gaaaaacgcc gtttcgctta
    27241 tgaaatgaac ggttaccgtg aaggtatcta tcacatcgtt aaagtatctt ctccatcatc
    27301 agcaaatgct gtcaatgaat ttgaccgtct tgctaaaatc aacgacgata tcattcgtca
    27361 catgattgtt aaagaagaag cttaattgtt gtttcatgtg aaacaataga aaacgatgag
    27421 aggagatgac tattttgatt aataatgttg tattagttgg acgtctgacg aaagatccag
    27481 atttgcgtta cacagcaagt ggtacggcag tcgcaacatt tactttggct gtgaatcgta
    27541 attttacaaa ccaaagcggc aatcgcgagg ctgattttat caactgtgtg atttggcgca
    27601 aatcagcaga aactttagca aactatgctc gtaaaggaac acttttaggt gttactggac
    27661 gtatccaaac tcgttcttat gataatcagc aaggacaacg tgtatacgtg acagaagtag
    27721 tagctgacaa cttccaattg ctggaaagtc gttctgcatc tgaaaatcgc caacaaagcg
    27781 gcggattcca aagttctggt caatcagctg gcggatttgg cggaaacaat aactctaatc
    27841 aaacatctca atcatccaac ggtatgcctg atttcgaccg tgatacttct gacccattcg
    27901 gctcaagttc aacgatcgac atttcagacg atgatttacc attctaaacg aatttgatag
    27961 gagggaaata gaatggcaca acaaagaaga ggcggacgta aacgtcgtaa agtagactac
    28021 attgctgcta accatattga atatatcgat tataaagata tcgaattact aaaaagattt
    28081 atttcagaac gcggtaaaat cttgccacgt cgtgtaactg gtacaggcgc taaaaaccaa
    28141 cgtaaaatta ctgttgcaat caaacgcgct cgtatcatgg gcttgctacc tttcgttagc
    28201 gaagactaat aaatcctagg aattgaatga ctgatccttt tgatcagcta ttcaattcct
    28261 ttttttctgt aaaaataacc tctattcgtt tagtcgctcg aatgacgaaa gttatggtaa
    28321 aatcaaatta gtcatatatg ggaggaaaat tatggaaaat aaaaacaatc atttatcccc
    28381 gaaacctctc ttccttatcc tgtttatatt aatcgaagta ttggtagtct taattttacc
    28441 atttcgtttt gttagcgctt cggtcattct attgatgaat ttatggggtg ttcgctattg
    28501 gttgaaaatg aaacgttggc ttattttaaa taaacgtgaa aaaatccagc aggcttcgac
    28561 tgtagctgaa accaatctag cttatgtctc agaagtcatg cctgtaggag tgattgctta
    28621 tcagccatca agccaaaaaa tcgaatggct caatccatta gcattgaaaa tcaaagatca
    28681 aagtggatta acagaggaag aattgctaga tacttttttt actgcaaaag aaaaaaagaa
    28741 aaaccaagtc tatctagcag ataaaacatt tcagtttgat atggatacag taaagggtgt
    28801 tgtttatttt aatgatatta cccaaaacca gttgttaaaa ctccatcaga cgaacagtca
    28861 gccggtgatt ggtattttat ctattgataa ctatgcagat gccattgata aattagatga
    28921 caaagagatt tcttatttga actctttcgt cactacacta gtttctgact ggatgaatga
    28981 ataccatgtc tttttcaaaa gaatcaacgc agaacggttt ttcttcgtag cacagatgat
    29041 cgatctgaaa aagatgatgg ataaaaaatt tgatttgctg gatcgctttc gagcagaagc
    29101 tgaaaaacag agctcgccta tcacccttag tatggggatt gcttatggct caagaaccct
    29161 cgatgaaatc ggtggagaag ctcagaataa tttagatgtt gctttagtcc gtggaggcga
    29221 ccaagttgtc gtgaaagagg taaaagaagg agcaaaacct gtttattatg gggggaaatc
    29281 tgctaagaat gtcaaacgca cgcgtgttcg ttctcgagcg atgagtaccg ctttgcgtcg
    29341 gatttttgat gaaacaggcg atgtgtttat catggggcac aagtttccgg atatggatgc
    29401 tttaggctct gcttttggtg tcagttgtct ggcacgcttc aaccagaaaa aagcttacat
    29461 cgttatcaat gagaacgaga tcattccaga tgtagaacgg tgtcttgaag aaatccataa
    29521 gcatcctgaa ttagaaatgc aactaatctc tccagaacgt gccatagagc tgaaaaaaga
    29581 agaggacctc ctcgtgatgg tggattacca taaaccttct ctatcgatct ctcagggcct
    29641 atatgagcaa tttgataaga ttgtgatcat tgatcaccat cgtcgtgggg aagagtttcc
    29701 aagtaaacct ctgctgactt atattgaatc ttcagcttct tcagcttcag agctagtttc
    29761 tgagctgatc caataccaaa gcagcaaaac aaatcgctta gatcgttttg aggccacatt
    29821 attgcttgca gggatggttg tagatacaaa tagtttcaat gtccgtacaa ctgcacgaac
    29881 ttttgacgtt gccagctatt taaggtcttg tggagcagac gcatccttag tacaatattt
    29941 attgagctcg gatttagcga catacttgga gatcagcaat ttgattgctg gaagcgacta
    30001 tgtgacttct gatattgttg tggcggcagg gacagacgaa aaagagtata atagtgttat
    30061 agcagcaaaa acagcagata cattgctttc gatgatcaat atcaatgcag ctttcgtcat
    30121 tacaaagcgg acagacggct tgattgggat cagtgccaga agtaccggca cgatcaacgt
    30181 ccaaatcatc atggaatcct taggtggcgg aggacacttc acaaacgcag ctactcaact
    30241 ggaaaatacg acagttgagg aaacaaaaca gaagctattt gctgcaatca aagaaaatat
    30301 gaatcaaata tatgataagg agtaatccaa atgaaagtaa tttttttaca agatgtaaaa
    30361 ggaaaaggta aaaaagggga catcaaagaa gttccagcag gttatgcaca aaattatcta
    30421 atcaaaaata acttggcaaa agaagcaacg aaaagcagta tcagtcagtt agcaggacaa
    30481 aagaaagcac aagaaaaaca cgaagctgaa atcttggaag aagctaaaaa gttaaaagaa
    30541 ttcttggaaa aagaagatac tgtagtcgaa ttgaaggcga aagcagggga agacagccgt
    30601 ttgtttggtt cgatcccttc aaaacaaatt gctgaagggt taaacaaaca atacaaaatc
    30661 aaattagata aacgaaaaat cgaattagaa aatccgatcc gttcactagg ctacacaaac
    30721 gtacctgtta agcttcatca tgaggtgacg gctacgatca aagttcatgt cgtacaagaa
    30781 taacaaatct tgatagcgat gttcagctgg taagtataac cagaaggaaa aaagacaaac
    30841 cgaaatttat ctgagaaaac agctgcagca atgaaagcaa aagctgcaaa tcgatttcag
    30901 attgatttcg gtttcttttt tgggaacagc aaaatagcat aaagaaaacg agtataataa
    30961 aagagaacta tttttttatt acactttcgg tacgaatgat gacttttctc tcattttctt
    31021 gtaaaatgaa gcaattgaag acaaggagcg atacgtatga atgaagtgtg gcaggatcga
    31081 atcccaccgc aaaatattga agctgaacaa gcggttttag gctctgtttt tcttgacgca
    31141 gaaacgatca ttgatgccat ggagtacata gagccaaaag acttttatcg tcgtggccat
    31201 caaattattt ttgagacaat gatcgagtta aatgatcgta atgaagcgat tgacgtggtt
    31261 actgtcaaag accggctgga acaagccaat ttgttagaag atgccggtgg tttaagttat
    31321 ctctcagact tggcattagc agttccgaca gcagcaaata tcgtctacta tgcgaaaatc
    31381 gttgaagaaa aatcattgct gcgtacattg atccagacag caacaggaat cgttacgaaa
    31441 ggattcgagc aaggagaaga agttcaaaca attttagatg atgccgaacg cagtatcttg
    31501 gaggtttcgg aaaaaagaaa tcgaagcggc tttttatcta ttgctgaagt actgaactcc
    31561 acgattgaaa atatcgatcg actagctcaa aacaatgaag aaatcacagg tctaccaact
    31621 ggctatcaag gtctggataa aatgacagca ggtttccagc ccgaagaact aatcattctt
    31681 gctgcacgtc cagcggtagg taaaacagcg tttgcattga atattgctca aaatgtagga
    31741 acaaaaacag atcgttctgt tgcgatattt agtttggaaa tgggggcaga atctcttgta
    31801 aatcggatgc tttgtgcaga aggttcgatt gaggccagcc atttacgtac aggtcagctt
    31861 tcggaagaag aatggtcgaa tctgatcgtt gcgatgggga gtctttctcg agcaaatatt
    31921 tttatcgatg atacaccagg gatcaaaatc tctgaaatcc gtgcgaaatg tcggaaacta
    31981 gcgcaagaaa aaggcaatct aggcttgata ttgatcgatt atttgcagtt gattgaaggt
    32041 aacggtaaag agaaccgtca acaagaagta tcagatatct ctcgccaact gaaaaaactg
    32101 gcgaaagagc tgaaagtacc tgtcattgct ctttcacagt tatctcgtgg agtagaacag
    32161 cgacaagaca aacgaccagt gctaagtgat attcgtgaat ccggatcaat tgaacaagat
    32221 gcggacatcg tagccttctt gtatcgagat gattattatg aaagagaagg cggagatgac
    32281 gatgatggaa acgagccaaa aaatgacaac gtcatcgaag tcatcatcga gaagaaccga
    32341 agcggtgcac gaggaacagt tgaactactg tttatcaaag aatacaataa gttttcgtcg
    32401 ctttcgccaa gagaagaatt ttaaagaaga ggttgtgagc cttgagcaat cccgagagct
    32461 ctcgtaagaa gatgcaagga gtaaccgcag gaacgactag agtacttaat ttaaacagat
    32521 ctccaaaggg attgtgacaa aagtcttgtc acagtccctt tttccgaata aacggtgttc
    32581 aatcagctga cccttggtat tagctcagca aacgtcaaaa tatgaggaac tattttgacg
    32641 tttgtttctc taatactcag gtcaaaacgc tgatcttacg acctcttttc cttaaacggt
    32701 gttctcctgc caactcctcg gtcacaagtc acaatatcat caaacatgag aagcagtttt
    32761 ttgaaattgc tccttgtgac tcagagttaa acggcaggct cacaacctct ttcttaaacg
    32821 gtgttcaaaa gcttgcatct gcggtaatac gcccaatcaa acgaaaaata tgagaagcta
    32881 tttttgtttg gctgttctta ttacttgatg caggacagct ttttcacaac ctctgattca
    32941 cggtgttcca aaagtagctt cttcgaaaaa taagtcgaaa tttcacaaaa atttgagaag
    33001 caattttcgt acattccttc ttatttcttg aagctgacca cttctgttac aacctctttt
    33061 ttaataacca ttctaaaaag ataaagttcg ttttttaagc gtttttataa aataaaaata
    33121 aaaatcattc gattttattt gaaatatgta tctattattg atacaatggt tcaggattaa
    33181 ataatataga acgaggtgtt cacatgtcat ctgtagtagt agtaggtacg caatggggcg
    33241 atgaagggaa aggaaaaatc acagactttc tcagtgaaaa tgctgaagtg attgcacggt
    33301 accaaggtgg cgacaatgca gggcacacaa ttaagtttaa cggtgtgact tataaactgc
    33361 atttgattcc ttctgggatt ttttataaag acaagatcag tgtgatcgga aacggcgttg
    33421 tagtcaatcc taaatcattg gttaaagaat tagcttattt acatgaaaaa ggtgtagaaa
    33481 caagtaattt gcgtatttct gaccgcgcgc acgtaatctt gccttatcat atcaaattgg
    33541 accaattaca agaagatgct aagggcgaaa ataagattgg tacaacgatt aaaggaatcg
    33601 gacctgccta tatggataaa gcagctcgtg taggtatccg tattgcagac ttattagata
    33661 aagaaatttt tgaagaacgt cttcgtttga atctagatga caaaaaccgt caatttgtaa
    33721 aaatgtttga ttcagaacca cttgattttg acgatatttt tgaagaatat tatgaatacg
    33781 gacaacaaat caaacaatac gtcacagata cttccgttat cttgaatgat gctttggatg
    33841 caggcaaacg cgtgttgttt gaaggcgcac aaggtgtaat gctggatatc gaccagggaa
    33901 cttatccttt tgttacttct tctaacccag tagccggtgg ggtaacaatc ggaagcggtg
    33961 tagggccttc aaaaatcaat aaagttgtcg gcgtctgcaa agcctatacg tcacgtgtcg
    34021 gtgatggacc atttccaact gaattatttg atgaaacagg agaacaaatc cgcgaagtag
    34081 gacgagaata cggtacgaca actggtcgtc cgcgtcgtgt aggctggttt gattctgtag
    34141 ttatgcgcca ttctaaacga gtttcaggta ttaccaacct atctttaaac tcgatcgatg
    34201 tattaagtgg attggaaaca gtaaaaattt gtacggctta tgaattagat ggcgaactaa
    34261 tctaccacta tccagcaagc ttgaaagagt tgaaccgctg caaaccaatc tatgaagaat
    34321 tgccaggctg gtcagaagac attacaggat gcaaaacact tgcagaactg ccagaaaacg
    34381 cacgtaacta tgtacgtcgt atctcagagc ttgttggtgt acgtatctca acattttctg
    34441 tcggacctga tagaacacaa acaaacatct tagaaagcgt ctgggcgcaa atctaaggat
    34501 aaaaacgacg actgaatcag gacgttctta ccagagcgag tcgaagtgtt tatctatacg
    34561 ataataataa cttgaagagg tagtgaaaga gacgtttagc cttattgccg ataggctgtc
    34621 tcttgaacac tgtttattca gaataaggga ctgtgacaaa aaccgtgtca cagtcccttt
    34681 ttccgaa
//
LOCUS       DAZKKW010000021        34649 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.021, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000021 DAZKKW010000000
VERSION     DAZKKW010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34649
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.021"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(92..940)
                     /locus_tag="ACG8PO_001010"
     CDS             complement(92..940)
                     /locus_tag="ACG8PO_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFQ0206354.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(1164..3104)
                     /gene="thrS"
                     /locus_tag="ACG8PO_001011"
     CDS             complement(1164..3104)
                     /gene="thrS"
                     /locus_tag="ACG8PO_001011"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFQ0206355.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(3441..3620)
                     /locus_tag="ACG8PO_001012"
     CDS             complement(3441..3620)
                     /locus_tag="ACG8PO_001012"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016862 - intramolecular oxidoreductase
                     activity, interconverting keto- and enol-groups [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxymuconate tautomerase"
                     /protein_id="HFQ0206356.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(3667..5490)
                     /locus_tag="ACG8PO_001013"
     CDS             complement(3667..5490)
                     /locus_tag="ACG8PO_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFQ0206357.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            5768..8047
                     /gene="helD"
                     /locus_tag="ACG8PO_001014"
     CDS             5768..8047
                     /gene="helD"
                     /locus_tag="ACG8PO_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase recycling motor HelD"
                     /protein_id="HFQ0206358.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(8140..8787)
                     /locus_tag="ACG8PO_001015"
     CDS             complement(8140..8787)
                     /locus_tag="ACG8PO_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0206359.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(8966..9685)
                     /locus_tag="ACG8PO_001016"
     CDS             complement(8966..9685)
                     /locus_tag="ACG8PO_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0206360.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(9816..10967)
                     /locus_tag="ACG8PO_001017"
     CDS             complement(9816..10967)
                     /locus_tag="ACG8PO_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HFQ0206361.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(11184..11924)
                     /locus_tag="ACG8PO_001018"
     CDS             complement(11184..11924)
                     /locus_tag="ACG8PO_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFQ0206362.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(11928..12251)
                     /gene="rsfS"
                     /locus_tag="ACG8PO_001019"
     CDS             complement(11928..12251)
                     /gene="rsfS"
                     /locus_tag="ACG8PO_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFQ0206363.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(12308..12901)
                     /gene="yqeK"
                     /locus_tag="ACG8PO_001020"
     CDS             complement(12308..12901)
                     /gene="yqeK"
                     /locus_tag="ACG8PO_001020"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HFQ0206364.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(12885..13535)
                     /locus_tag="ACG8PO_001021"
     CDS             complement(12885..13535)
                     /locus_tag="ACG8PO_001021"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFQ0206365.1"
                     /translation="MMNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHL
                     VMAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIEL
                     ARGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPG
                     YTTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(13545..13856)
                     /gene="yhbY"
                     /locus_tag="ACG8PO_001022"
     CDS             complement(13545..13856)
                     /gene="yhbY"
                     /locus_tag="ACG8PO_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFQ0206366.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(13872..14981)
                     /gene="yqeH"
                     /locus_tag="ACG8PO_001023"
     CDS             complement(13872..14981)
                     /gene="yqeH"
                     /locus_tag="ACG8PO_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HFQ0206367.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(14978..15505)
                     /locus_tag="ACG8PO_001024"
     CDS             complement(14978..15505)
                     /locus_tag="ACG8PO_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HFQ0206368.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(15685..16470)
                     /locus_tag="ACG8PO_001025"
     CDS             complement(15685..16470)
                     /locus_tag="ACG8PO_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HFQ0206369.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(16463..17332)
                     /gene="accD"
                     /locus_tag="ACG8PO_001026"
     CDS             complement(16463..17332)
                     /gene="accD"
                     /locus_tag="ACG8PO_001026"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFQ0206370.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(17333..18712)
                     /locus_tag="ACG8PO_001027"
     CDS             complement(17333..18712)
                     /locus_tag="ACG8PO_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFQ0206371.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(18720..19139)
                     /gene="fabZ"
                     /locus_tag="ACG8PO_001028"
     CDS             complement(18720..19139)
                     /gene="fabZ"
                     /locus_tag="ACG8PO_001028"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFQ0206372.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(19164..19640)
                     /gene="accB"
                     /locus_tag="ACG8PO_001029"
     CDS             complement(19164..19640)
                     /gene="accB"
                     /locus_tag="ACG8PO_001029"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFQ0206373.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(19643..20878)
                     /gene="fabF"
                     /locus_tag="ACG8PO_001030"
     CDS             complement(19643..20878)
                     /gene="fabF"
                     /locus_tag="ACG8PO_001030"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFQ0206374.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(20897..21634)
                     /gene="fabG"
                     /locus_tag="ACG8PO_001031"
     CDS             complement(20897..21634)
                     /gene="fabG"
                     /locus_tag="ACG8PO_001031"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HFQ0206375.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(21646..22563)
                     /gene="fabD"
                     /locus_tag="ACG8PO_001032"
     CDS             complement(21646..22563)
                     /gene="fabD"
                     /locus_tag="ACG8PO_001032"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFQ0206376.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(22670..22894)
                     /locus_tag="ACG8PO_001033"
     CDS             complement(22670..22894)
                     /locus_tag="ACG8PO_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFQ0206377.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(22947..23909)
                     /locus_tag="ACG8PO_001034"
     CDS             complement(22947..23909)
                     /locus_tag="ACG8PO_001034"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFQ0206378.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(23919..24359)
                     /locus_tag="ACG8PO_001035"
     CDS             complement(23919..24359)
                     /locus_tag="ACG8PO_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005471259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFQ0206379.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
     gene            24665..25630
                     /locus_tag="ACG8PO_001036"
     CDS             24665..25630
                     /locus_tag="ACG8PO_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFQ0206380.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
     gene            complement(25687..25917)
                     /locus_tag="ACG8PO_001037"
     CDS             complement(25687..25917)
                     /locus_tag="ACG8PO_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HFQ0206381.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(26003..26890)
                     /locus_tag="ACG8PO_001038"
     CDS             complement(26003..26890)
                     /locus_tag="ACG8PO_001038"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFQ0206382.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            complement(26979..28202)
                     /locus_tag="ACG8PO_001039"
     CDS             complement(26979..28202)
                     /locus_tag="ACG8PO_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010706866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFQ0206383.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(28219..29265)
                     /locus_tag="ACG8PO_001040"
     CDS             complement(28219..29265)
                     /locus_tag="ACG8PO_001040"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFQ0206384.1"
                     /translation="MKVLLYFESEKMLAKSGIGRALDHQKRALTEVGISYTLDEKEDY
                     DILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQLSPIVKKYLVGL
                     YQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQKFREYFKLSPD
                     QKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIRKIVKEDHPENV
                     LFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVRDIPVYNGWLED
                     SKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEELKNVYESVLNE
                     KVSSKLKKVNQVKD"
     gene            complement(29429..31156)
                     /gene="ptsP"
                     /locus_tag="ACG8PO_001041"
     CDS             complement(29429..31156)
                     /gene="ptsP"
                     /locus_tag="ACG8PO_001041"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFQ0206385.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRGLMKRLDTAKMAELADRALKECDTMEEV
                     VELVHEYVK"
     gene            complement(31156..31422)
                     /locus_tag="ACG8PO_001042"
     CDS             complement(31156..31422)
                     /locus_tag="ACG8PO_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HFQ0206386.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            31901..34135
                     /locus_tag="ACG8PO_001043"
     CDS             31901..34135
                     /locus_tag="ACG8PO_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0206387.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            34283..34603
                     /locus_tag="ACG8PO_001044"
     CDS             34283..34603
                     /locus_tag="ACG8PO_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HFQ0206388.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPQH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
BASE COUNT         9788 a         7491 c         6050 g        11320 t
ORIGIN      
        1 cttaagtcgt gaaacacttt gatggatcat ttatttgtgg aaaactacgt tttgttattc
       61 taaaaaactt atataaaaaa tagtaaggtt tttatttcga atccataaaa tattcttgga
      121 ttgcttctcg tgtttttttc actttttcgc taagcttttc gtttttagcc atatagaaat
      181 aaaaaatgat atcgatcgta gcaaattggg ccaaaagtga attagttgcc gcacttcgat
      241 tcgtgatttc ttttggtcta gatgtttgta cgagtacatc ggctatcttg ctaagcgggt
      301 tatttccaaa ttgcgtcaac gcaatgattt cgatattcat gcttttcgcc agctcggcta
      361 atgcgactac atctgcactt ctacctgagt ttgaaacaag ccaaagcact ttctttttct
      421 cattgctgac aagttgcggg agcaagacgt ggatatcttt ttcaaaaatg attggcttac
      481 cgacacgaga ccatttttgc agaatatctt ctactactaa agaagaggcg ccaaccccat
      541 ataaaaaaac agtctctgcc gattccaaac actctacgac tttttggatc gtttcttctt
      601 ctagaatatc tactgtttct tttatcgcta cttgtgcatt gttaccaagt ttgtttttga
      661 tagagtaaaa ggactcattt gcttctatat cactaaaacc ttcatcgatt ccagtctgta
      721 gttctgttga aatcgtgatt ttgaagtccg tcagactttg aaaaccgatt tttttggaaa
      781 aacgtacaac tgtcggcgca ctcaacccgg aagcttctgc taattcatta gctgtcattg
      841 tagggacctt ctcaatatta ccgataatgt agttggccaa ctttttttct gctgaagaat
      901 aatcttccat catactttca atacgactaa ttacgctcat aagttcctcc acccttttaa
      961 tgatagtcta tgtaagtata ccatatcaga cccttattca cggtcgtata ctggataaaa
     1021 aacaaaaaaa caatccgccc aacagaatat cttatcccat caatcccaca gattctattg
     1081 agcatctgtg tctttgagga atacctgtta tctattgttc ggattgcttt ctatctacta
     1141 cttttttaca ggaaatttac ctattagttt tctctgctgt aatttttgac ttctgcttcc
     1201 atagcatcaa taaagatatt caaatcttct acagctgttt ctttacttcc ataacgacgt
     1261 acgttcactg ttgcatcttc caattccttg tctccaacaa ctaattggta agggatcttt
     1321 tgtgtttgag aggcacgtat cttatagccc attttttcat tacgatcatc aacttctaca
     1381 cgcaacccct tcatttgcaa acgttctttg atttcataag cataatcgcc atgtgcatca
     1441 acagaaacag ggatgattgt tgcttgtata ggtgccaacc atgttggaaa agcgcctttg
     1501 tatacttctg ttaaataagc gacaaaacgt tccattgtag aaacaattcc gcggtggata
     1561 actactggac gatgcgtatt ttccccgtct tctccaacat atgtcaaatc aaagcgctct
     1621 ggcaataaga agtctaattg tatagttgat aatgtttctt ctgtaccaag agctgttttg
     1681 acttgaacat ctagttttgg tccgtaaaag gctgcctcgc cttctgcttc aaagtaatct
     1741 agttctaatt catccatagc ggctttcagc attgcttggg ctttttccca cattgcgtca
     1801 tcatcaaaat atttatctgt attgtttgga tcgcgatagc tcaagcggaa acgataatct
     1861 gtaatattga agtcagcata tactgccacc atcaattcaa gcgtacgttt aaattcatct
     1921 ttgatttgat caggacgaac aaatgtatga ccatcattca acgtcatttc tcgtacacgt
     1981 tgtaaacctg acaaagcacc tgatttttca tagcgatgca tcattcctaa ttcagcgata
     2041 cgaattggta attcacgata agaatggatc gtgtttttgt agaccatcat gtgatgaggg
     2101 cagttcattg ggcgaagaac aagcatttcg ccatctccca tatccattgg cgggaacatg
     2161 tcttcttgat aatgatccca gtgtccagat gttttgtaca actcaacatc tcccattatt
     2221 ggtgtatata catgttggta acctagacta acttctttgt ctacgatata acgttcgatc
     2281 gtgcgtcgga ttgttgcccc ttttggaagc cagaatggca aaccagaacc tacttctggg
     2341 gaaaccatga acaagtcaag ttcttttcct agtttacgat ggtctctttc ttttgcttct
     2401 tcacggagac ggatatattc tttcaaagct ttcttgtcaa aaaaggctgt cccgtataca
     2461 cgttgcatca tttgattgtt tgagtttcca cgccaataag cacctgccac tgataataat
     2521 ttaaatactt gtatacggcc agtggatgga acatgtggtc cacgacacaa atcgatgaat
     2581 tcgccttgtt gataagcagt aatcacttct tcttctggca attcactaat cagttctact
     2641 ttgtacggat cacttgcaaa aatttctaat gcttcctgtt ttgaaagaac acggcgttcg
     2701 atcggcagat tttctttgac gattttcatc atttctgctt cgattgccgg caaatcttct
     2761 gcagttactt ggttttgtcc attatctgta tcgtagtaaa accctgaatc gatagctggt
     2821 ccaacgccaa agtggatatt tggatagagg cgacgcatgg cttgggccat caaatgagct
     2881 gctgaatgac ggactaagcc aagtgcatct tcatggtctg gtgtgatgat ctcaatactt
     2941 gcatcttcct cgattggacg agtcaaatca acgagttttc cgttcacttt accagctaaa
     3001 gctttttttg ctaagctttt tgaaatgctt tcagcaattg ccaatgttgt tgttccagac
     3061 tcaaattctt tgactgcacc atctggaaaa gtaattttta tcataatagt ttcctccttg
     3121 aaatgttttg attgatcaga tcatcaagct gaaaaacaaa aaaatcctag catctcttga
     3181 aaagagatac taggacgaaa agttcgtggt tccacctaat gttcaaacgc aaatgcgttc
     3241 ctcaagcgtt gtaacggtcc gaccgccctt gcttcaacaa gggtttcgaa agtggtcatc
     3301 ctttggtttt ctcctggaaa agtttcagcc tagcttttcc tctcttatcg aagactctca
     3361 aattcagttg gctttctcat caatcaatta tgtgtttatt taaccactaa taaatagaaa
     3421 aatcaagtgg gaaactaaac ttattttttc cattccccat ttacaccgta tgttcctttt
     3481 ttcatctcat tgatgatgac atggatgtgt tcttttggtg cctgcgtgtt tttatgaaca
     3541 gcttccgtga tatctttcat catattttct aattgttccg gactgcgacc ttctactaat
     3601 tcgacatgta cgaatggcat aatgctctct cctctttctg atcgttcttt tttcaactcg
     3661 attctatcat actatgtttg tcttgggtac tgaatcagga aaaatggctg gatctttttt
     3721 tgctctcaag gcactcaaaa tagagaccgt atccacgact tcttgcaaaa cagctccgaa
     3781 taatgcgggg attagtcccg tacttgcaat taacatcaaa aaaatacaaa taaagatgcc
     3841 aatcagtaca gactgtttcg ctattctcat cgtatctctt gaaatcacta ctgcttttgc
     3901 tacacgcaag agatcatctt tcaagatgac cgcatctgca ctttcactag cagctgtaga
     3961 accatgagcc cccattgcaa taccgatatc tgcagcagca agtgctggtg cgtcattcac
     4021 gccgtctcct accatcgcaa caggtctttg atctttaggg atactttcta aaatggtcag
     4081 tttatcttga ggcaagctac gggcatgtac agaatctaat tggacttttt ctgcaatcgt
     4141 ttgagcaatg gcagatccat ccccggtgat catcatcaaa tttttcacat caagttttct
     4201 tagctccttg attgtctttg ctgcttctgg gcgaatctga tccgaaaata ggatcgttcc
     4261 tgcaaaagat cgatcaattg ttacatagat tgctgtattg gacgtttcta tttcttcagc
     4321 tgaaacaaaa gaggcacgtc caatctttac ttcatgatta ttgactactg cttggattcc
     4381 ttgaccggtc acttctttca gttgactaac aggatacagt tggctgtttt tcacgttgtt
     4441 cactaacgaa cgagctaaga tatggctaga ttcctgttct gcactagctg catacagaag
     4501 tagctcttct ttttttattc cattgactgg ctgaatcgcc tctacttcca atatcccttt
     4561 ggtaagcgtt cctgttttat caaaagcaat cgtttttgta cgtgcgagct tttcaatcgt
     4621 tgttccagtt tttacaacga tcccgttttt acttgaacgg ctcattcctg caactaaagc
     4681 aatgggcgca gctagaatca gtggacaagg tgatgccact accagtacct cagcaaatcg
     4741 taaagggtct tttgagaacc accaagaaat accaccgatc aaataagcaa tcaacgtgaa
     4801 agggactgca taacgatcag ctaaacggac aaatcgggca ggcttttctt ttgattcttc
     4861 gactagttta ataatcattt gatactggct gtctgcggca cgtttttcag cgatcatttg
     4921 gatcgatccg tctccattta aagaccctga cataatctga tctccaggtt tcttggtttc
     4981 cgggcgggac tcacctgtca aagaagattc atcgaccgaa gattctcctt tggtcactcg
     5041 accatctgcg ggtacaagtt cgccaggctt gactagtaac tggtcatcaa tatttatttc
     5101 atcgattact atatcctgca attttccttc tttgaatcga tgggcaattc ttggggtgtt
     5161 atctaacaaa gactgcagtt ctttacccgc tcgtttattc gcgtaatcct ctagactatc
     5221 tccgcccgta agcatgatca atatcatcaa actagcccag tattctttga cggctaatgt
     5281 tgcaaggatt gcggtaatcg ctaatacatc tacgccatat cttcctgaac gtaacgtctt
     5341 gatcatttcc aaaaacatca agaaggcgag aatccctcca gtaaatgcga caatcgcaaa
     5401 tgcaagggcc ggctggtgaa aaacaaattc acttaataaa gctatcccac caataagcaa
     5461 agtaagtaaa aattttttga aatgtgtcat tgttttttct cctcttttat gtgacttaga
     5521 gaggttagta ataaatttcc agctgcctct ttcatcttaa gcatagtcct tgactagcac
     5581 tttttaaaac aataggcctt aattgaaaac aactatcact atcagcgttt ccaaaattca
     5641 tcagttctac aaacaaaaat attcagcaag gattactttt tcgattcatc agttaaaaag
     5701 tacctttttt cgaaaaacta tgctatactt ttaattcgtt atttacggcg gaaggagcaa
     5761 acatgcgatg aatgagaaac aactagaaca aacgcacgtg gatgaaacga tcgaactaat
     5821 ccagatcgaa caaaatatgc tggatacaaa acaacaagaa ttgactcagg aaatgaatca
     5881 ttcgacaaaa gacacagcta atcacaagat tcgagctggt tccaatgaag ctttttatga
     5941 atctgctgtt gaatatcgcc aacatgagca ggaattgctt ttgaaatacc atactcttga
     6001 agcacagcaa aaacgcctgc aaacgttgtc tgtcatgaaa gatagtcctt attttgcccg
     6061 tatcgacttt aaagaagacc gtgaaacaga aacattatat ctaggtatcg cttctttaag
     6121 agatctggca gaagaaacaa tcgttattga ctggcgcgca ccaattgcca atctttatta
     6181 tgaaggtgag attggtcccg cattttatga aactgaaaca gatagaatag atgtcgatct
     6241 gcagttgaaa cgacaattta aaatccaaga aggacgtatt ttatctatgg tagatacgtc
     6301 ggaagtaatc aatgatgact ttttgctaga gatattggat gaatcatcaa gcaaccatat
     6361 gaaaaacatc gtctctacga tacaaaaagc acaaaatgca atcattcggg acacaacaag
     6421 taaagtaatg ctgattgaag gaatcgccgg aagtggaaag acatccgctt tgcttcaacg
     6481 tattgctttt ctgttgtatc ataatcgcaa gtggctcgag gctgaaaacg ttcttctatt
     6541 ttcacccaac catttgtttt ctgattacat ctcgactgta ttgccttcat taggtgaaag
     6601 cggcgtgcct acacagacat tcaaaaatta tttggtgcag ctgctgccag agttttcttt
     6661 actagatgaa cagcaacaag aaagtggttt tttgctaggg gaaaaagatc cgatacaagt
     6721 gatgaaaagc ggattgacct tagtcgatca gattgatcgc tatatccaag gcatcacaaa
     6781 ctacggtccc ttgttccgtg atatgaaaat aaatggccgt acgatcctat caaaggaatc
     6841 gattcgccaa tggtataaag aaacaaatga acaattacct ttacatcata gactttctct
     6901 attacaaacg aagctcctga aaaaattagg cggtctgcaa aaagacgaaa cacgtcagca
     6961 atgggtcaaa gatctggcgg aagaacagct ccaagaacta tacgcaacag atccaaatct
     7021 tgaatataca gaacaaaagg agcgttcatt acgtaaaaaa ctggccaacc aaatcgtacg
     7081 taaaagattc cgtcgaatcc atcgagggat catgaattat caatttgtca atataacgaa
     7141 acaatatctc cactttctac aaactgtgcc actttctctt ctaacacgac ataaaatcag
     7201 cgagaccagg tggcaggaac atttgacaac tgttcgtaca cacttgcgtc aaagagaatt
     7261 gcgtcaagag gatgctgtac tattcttttt actgatgcgc cgaacatatc cagtgacggt
     7321 tacacacaaa gctcgctata tctttattga tgaaatgcag gatttcgcac catcacaagt
     7381 ggcattgctg caagccttgt tcccaaaagc aaatttgact ttctgtggag atctaaatca
     7441 aaatgtcttt ggaaatgaaa cgatcgttgg atcattggaa gaccttttcg tggaccgtga
     7501 agtcactcgt ttccaattaa cgacaagcta tcgctccaca aaagaaatca ctgattttgc
     7561 taatcatttt attgtgaaag aacatcaagt agaaacaaca gcaagaaaag gagaccttcc
     7621 tttgctcatc caaggaacaa cagatgatat caaattgaat tggttagcag ataccattcg
     7681 aacgactcag ggaaaagccc ggtattggcg tacagcaatc atagctaaaa cacaagaaga
     7741 atgcgaagct ctttatgctc aactgccaga cgagttgaaa caacaagtcc agctgatttt
     7801 aaatgagagt gactttatga agcgttccat tattttactt ccagcctttt tagcaaaagg
     7861 tctggagttt gaccgagttt tcttgtggga tgtagatgca gaacattttt cttctcagca
     7921 agacaaattg atcctttaca ccatgtgtac acgtgcgatg catgaattga ccctttttac
     7981 ctccaataat gaaagtcctt tgttaaacaa tatggatatt tcatcttatg agaaaaaagt
     8041 actgtaagta aaaagacaac tgctatcatt aatgttagca gttgtctttt tatttacaat
     8101 aataattgca gaaaaagtaa ttcaatcatt taaggtcatt tattctatat ataaatccaa
     8161 atctgtttca ggatatttta atttgacaag cgaaataatc attttcagtt cttccatgtt
     8221 ttctggatat tctgggtcaa ataccttgct atgacggcca atcccgccat tgtcgatcaa
     8281 tcggtaaatt cgattccatt caatgaattg gtcaattgat tggaaattgc tgattggata
     8341 gccgccacaa tccaaaatct ctgatacacg aaatgctagg tatgcactat cgatcacttt
     8401 attttttttc gtgtctatct tctcatttct ttcgataaga attttttcag gacgatgttt
     8461 caagctccac tctaatagta gaaagaaagc ctccatccga tactcttctt caaataaccg
     8521 aaaggctgaa tcagaacgac gaaaaagcag cggaatcaga taattttgtc tataaatcag
     8581 cattggtgcg cggtccacca tacactctaa ataacggttc agataccctg taccgacata
     8641 gtttcctaat cgatagcctt caacgccgaa tcttgcgtat ttttgcgcta attttgaaca
     8701 atcagttacg ttcacagcta actcttctac tttgcggtaa ccgcttaaga tacgttcaaa
     8761 aggttctaaa gtgatttttg cttccatatt cttccccgtc catcttccac atacttcata
     8821 tcatttcaca tgatacttat gctctcatca taccacaggt tgaattagaa aagaaaataa
     8881 atcacacaaa aaagcaagac aatctgttag tgagattgtc ttgcttttcg aaaaagactg
     8941 aatacttttc gttaaaactg gttgcttagt cttctaatgc cacattatgg aacacatgct
     9001 gtacatcttc tagatcttcc agtgcatcaa tcattttttc aaatgtttct tgatcttctg
     9061 gagatagtgt tacctcgttt tgagggatca tttggatctc agcgattgaa aaatcagtga
     9121 ttcctttttc ttttaatgct tcttgtatac tatggaaatc cgctacttca ccgtaaacga
     9181 tgatttgtcc ttcttcttcc attacatcgc gaacatcgat gtctttttcc attaggtatt
     9241 caaggatctc gtcagcgtct tctcctgcaa aagcaaagat tgctgtattg tcaaacatgt
     9301 aagaaaccgc tccggaaaca cccatgttcc cgccattttt cccaaatgct gcccgtacgt
     9361 ctgctgctgt tcgattcaca ttgttcgtca atgtatcaac aatcaccatt gagccgcttg
     9421 gtccaaatcc ttcatagcgc aattcagagt atgtttcatc tcctgcacct tttgcttttt
     9481 cgattgctcg gtcaataata tgttttggta cattataagt tttagcgcga tcaatgacga
     9541 aacgtagttt ttggtttgca tgcggatcag gatcacctga ttttgctgcc gcgtagattt
     9601 caatcccaaa ttttgcataa atccggctat tattagcatc tttagcagtt ttctttgcta
     9661 cgatatttgc ccatttacgt cccatatttt cacttcgctt tctgatttga tagtttcata
     9721 acgcaaaaac gtcctgttcc attatacttg tgaatcacga ttctgtgaag ctttttttct
     9781 ttcttccttc attcttctca tttttagtca attatctata ctcggattgg gatttgcccg
     9841 aaattctgtt ccggaatatt gcctcccaat tgatagattt gatcacttct aactaacagt
     9901 tccgcttctt gtgcctcttt ttttccgatt cgggaataaa tagaaatctc ttttttttct
     9961 tttaagaatc ttcttccagc atcggtaaat ccaagtagtc gaagataatt tttattttgt
    10021 tggacaggaa tctcttgctt tttgatattc agcaagacat aacaagataa tcgttggatc
    10081 cgtgtccatg tatatctttt tgtttttacc tttgacacaa aatcctcaaa actatcagca
    10141 gttgcagctg ctgttttcat gcgcgattct attccttcgg tcatctgata gatatctgtc
    10201 agttccagca aagtagaaga taacaaccga taacgcaaat acggccaata agattcccaa
    10261 gtctgcagcg aacaagttga aagatcttct tctgtctgag gcggcacaag tccagctatt
    10321 ctttttcctt cttctaacgc atggcgaatg gctgttgcac tggctatcct ctgatctgtg
    10381 atgatttttt catgatagcc tgcactttta cgtcggatcg gatgtaattc catagggtgg
    10441 acatacttgg cattttcctt tgcataactt aatcccaaga tatgatttgg cgaagaaaaa
    10501 tcaagcgtaa tatcgggata cagactgcga aaaacctcag tcattttttg cggataacta
    10561 tacgtttgat cagacagttc ttgaaatgcc tcttctatcc tatcttgatt ctcttggaca
    10621 aagcgtccga aactgctgta atcaaacgaa gtctcttctt ctgttccaaa acacaaactg
    10681 tcacagtcca gtgtttgcaa gatttttact gcacctttag caaaatagtc agctgactga
    10741 accgcccatt cgacgggaag ctccactact aaatccacac cattttgtaa agcttcattc
    10801 gcgcgatgcc atttatcgat gatagccggt tcgcctcttt gcaaaaaatt cccgctcatt
    10861 acagcaatga cgacctctgc tcctgttatt tttcgtgcca tttccacatg atagcgatgt
    10921 ccattgtgaa aaggattgta ctctacgata atcccgcagc ttttcatgcg catcctcctt
    10981 catagttatc gataaaaaag aaatagagac aagtaaaaaa aggcaaaaat cggaaaaaat
    11041 acccgaacta tataaactat tctcgcaaac acaatacttc tgcgacgaaa agctgcactg
    11101 caagagcaat gcctatatcg ttcggatata taatttatgt tgaagattta ttcttttctt
    11161 gttaactcga gcaggcaaaa ccgctagtct ttgtatgcta caaagaacca gcgctttgat
    11221 ttctctgatg ctgcttcgtc tgtaaaatca gaaaatgctt cgactttcga aaagccgctg
    11281 ttttccagca ttcttaaata actttccaat ggataggtac gctcttgatg tagttcatct
    11341 tcacgaataa atcgaccaga cccttcttgt tcttcaacga aaaaggtaag aaaatgttcg
    11401 atgctgtgtt tctcgcttcc tggataacta tcccacaaaa aagcaaaatt atccccttga
    11461 taatgatagc tatattctgg aaaaacctca tctacttgat aggtggagtg tacgtcaaat
    11521 aaaaatcggc cattttcttc taacgcttga tagacttcat caaacacttg ctgtacctct
    11581 tgtctatttt tcatatagca cagcgaatca gaaaaacagg taattgcctg atattgtccg
    11641 atttctgata aatcgagcat attgccttcc acaaattgga tcgatgcgtc ttcttcatat
    11701 gctcgctggc tggcaatcat caacatctct tctgaaaggt ctaaccctgt cactttatat
    11761 ccatcttttg caaaggcaac cgctaatgct ccagtaccgc atgccagttc caaaatattt
    11821 tttgtgtctt cttgtaaatg acgtttcgaa aaagctaacc attgctgata aagtgtttcg
    11881 tccatcactt catcgtaaat gaaagcaaat gtttcataag ccatatgtta atccaaccat
    11941 tcactaagat cgactagcgg agcatctgac catagttttt ccagattata gaaatttcgt
    12001 tctggcgcat ggaaaacatg tacgatcaca tctccaagat cgatcaatac ccattttccg
    12061 ccttcttttc cttcaatacg ttttacttca tatttgtttt cttcttcttt atcaatgatt
    12121 tcttctgtaa ttgcgttgat ttgacgttcg ctatttgctg aacagatcat gaaataatct
    12181 gccaacaagg aaacatttcg aacgtctaaa gcgataatgt cttctgcccg ttttgaatct
    12241 gctgcttcaa tcgcaatttt taattgttct ttactatcta tgatgatttc ctcctaatat
    12301 gagcagctta tttttttgct acccagtaat tatatgtttc gattgttttt ggataaatct
    12361 gttgttcttg cgcaagcaaa tgggccaatg tatgttttgt ttcaaatgct acagcttcgt
    12421 ctagatcagc ccatgcaatg gctcgtgcat cttgtacccc aggaaaatct cgacctggtt
    12481 caatatagtc tgctacataa atgattttgt caaggagact catcttagca gccccagtcg
    12541 tatgcacccg gattgcatga agaatctctg catcggtgat tcctaattcc cgctctacaa
    12601 acgaagcgcc aacaagacca tgccagattg cattattata attcaataac tccggatcaa
    12661 aaccgtctct tacgattacc attttgaact cctcatctgg tcgttctttt gcataatcat
    12721 gtgtcaatgc agcaatacta gctttttcag gagaagcacc gtattttttg gcaagagcca
    12781 cggctgtttc ttctactcca agcacatgct tgaagcgttg ttcgctcatt ctcatctgta
    12841 ttttttgcat caggttttct ctagaataag cagtatattc attactatag tccatattca
    12901 tacaaccctt cattttgaat ataatcaatt actttatctg gaacaaggta gcggatagaa
    12961 cagccttctt tgatcttttg acgtattttc gtggaactga tgtcgatctc aggtacatct
    13021 acccaaatta caggatatgg agtatccgtt gtgtatcctg gtcgacgtat acccacaaag
    13081 ttgaccatag aagtcagctc atcgatttta taccattttg gcaaatattc taccatatcc
    13141 ccaccgataa taaagtaata atctgtatcc ggattgtttt gcgtcaattc cttcatagta
    13201 tcataggtgt aacttttccc accacgagct aattcaatcg tttcaatctg caaaaaagga
    13261 ttgtcttcca ccgctaattc caacatgttg agtcgatgtt ttgcatcaat cgtctgtttc
    13321 tcatcaacat gaggtggctg ataagaaggc atcaaaaata cgcggtcaag acctaaattc
    13381 cggccagctt gttcagccat cactaaatgt gcaaggtgaa cgggattgaa tgttccgccc
    13441 aagatcccta cttgcttttt cttctctaag aattgaggca tttcttcagg aaataattgt
    13501 gaacgaacaa atgtcttagc ttgcgtattc atcatggtca ctccttatat tgcttttact
    13561 tcttttgata gcctttgata cttttctttt gtggaaggtt tgtataatac aataacacgt
    13621 ccaatgattt ggacgatttc gcaatctatt tctttttcta atacttgagc tgcttcttct
    13681 gcaatctcat ctgtattttg aagtaacgat actttgatca gttctctttt ttctaaagct
    13741 tcttcaattt gaacgatcat tgctgaattg atcccacctt ttccgatttg aaaaatcggc
    13801 tgaagatgat gcgctttgct acgtaaaaaa cgcttttgtt ttcctcttaa actcataatt
    13861 ttccttcttc cttaaatcaa tgctttgcgt ttcaatacat ctacaccctt tggcgcccag
    13921 cccgcaatta ctactggcgc tgttacagtg atccagccta gaccggcaaa aacgatgtct
    13981 gttttttctt tcacagaaaa ctcaaaacga acaagctccg gaaatgcctc gacttcatca
    14041 ggacgaggag gctgtaataa tccacctaca tgtttttgat aaaattcgtc tgctttttcc
    14101 agtttcgttc gatgaatatt tacatcattt gacacataag caataaatga agcacgattg
    14161 ccgctgataa aatcaaatct tgcaagtccg cccaagaaaa gtgtttgccc ttcattcagc
    14221 tgataaactt taggtttgat ttcttttaac ggtgcaatga tccgcaaatc ttttttacct
    14281 aagtagtgcg ccatttgatg gcgatggata attcccggtg tatcaatcaa aaaatgcccg
    14341 tcatctaaag gaatctcgat tttatccaag gtcgttcctg gaaactgcga agttgtgatt
    14401 acatcttgta ctcccgctgt atttttgatg atttgattga taagcgtcga ttttccgaca
    14461 tttgttactc cgacgatata gacatcccgt ccatcgcggt attcttcgat tttttctaat
    14521 agttccgtca tctcatgaga tttcttagca ctagttaata atacatctac tggacgaatt
    14581 cctgcttcgt aagcacgttc cttcatccat tgtgttaatt tagttttttt caatgattta
    14641 ggtaatatat ccactttgtt cccaactaaa agtactggat tgtcaccgac aaaacggtgt
    14701 agtcctggga ttagtgaacc attgaagtca aaaatatcca ccacattgac gatcaatgca
    14761 tctttttgcc ctaattcatt cagtaagcgc aaaaagtcgt catccgtcaa tgagacatct
    14821 tgggtctcat tataatggcg taatctgaaa caacgctgac aatagacttc cccggaagct
    14881 aaaccttttt ccaaagccgt ttgcggcgtg tatcctaatt caccgggttt gtttgtttgt
    14941 ataacggcac cacagccgat acattgtaat tcttctgtca ttcgattcca cctcgccagc
    15001 ccatatcagg gtgttttgct aatagatagg ccataattct tttttcccaa aaacgattaa
    15061 attgtgtctt ccaactatct gttgcgataa tgggttttac aaggatgcta cgtatccctg
    15121 cacgattggc tccacgtata tcagtcataa tctgatcgcc taccattact atctcatcat
    15181 ctgctaaatc caattgtttc tttgctatgt tgatccctct tgctaaaggt ttcattgctc
    15241 gtgcaacata caaaagatca aatttttcaa tcgctcgcgc gacacgtttt gaattattgt
    15301 ttgaaacaac aacaacaggg attcctgcat ttttcatttc taaaatccaa gtaagcaact
    15361 cttctgttcc atctggatta ttccatgcaa tcaacgtatt gtctaaatcg gtcaatactg
    15421 ctttgatgcc tagattcttt aattgggcag gtgtaatctg atagatggct tctaccatcc
    15481 atgttggttt gtaatttgaa aacatgctgt ctcctcatct gcaaaaaatg aaggagcaca
    15541 taaagtactc ctcaaccccg tattatactg catatcagca aaaaaaacta cctctatact
    15601 cgttcgtctt tcagaaaaag catttcttta caaaatgacc aaaaataaag attgatcaca
    15661 gaaaaattta gaaacaaaaa acaactaata ttggcggaac ctcttgtatc gtttttccag
    15721 taatttatgc tgatctaatc gaacaagttc cgtcaatctt tcaatcaacg cttttttcat
    15781 aatgtgtgcg actttatatc gtggcaactg ctcgccgttg aacgtttcag gaataactcg
    15841 atcaacgatt cctaactcgc gtagttctgg cgcagtaatt ttcatcaact cagctgcttc
    15901 tgcggcgcga ctaccgtctt tccacaaaat cgacgcaaat ccttctggag aaagcacagc
    15961 ataaatcgtt tcttccagca tccagacttc atccgctaaa cctagagcaa gggcaccgcc
    16021 acttccacct tcaccaatga tgatggcgat gatcggtacc tttaaatcag acatttcgag
    16081 cagatttctg gcaatagcct ctccttcgcc gcgctcttct gctcctacac cgcaatatgc
    16141 acctgctgta ttgatcaagg taatcaccgg tctattgaac ttttccgctt gtttcatcag
    16201 tcgtaaagct tttcgatagc cttcaggatg gggagaacca aaatttctgc ggatattttc
    16261 ctgcagatcc cgtccttttt gaataccgat cactgttacc ggtttgccat ctaatcgagc
    16321 gattccacca acaatcgctt cgtcatctgc aaataaacga tcaccatggc attcctcgaa
    16381 agaatcgaag atctcttcaa aaaaatccaa gcttgtccat cgttctttag ctcttgctaa
    16441 ctgaacgaca tcatgtgctg tcttatccac gatctatcca tcctttctgt gtatgcaatt
    16501 caagaagcgt atggattttc tgtttcaatt ccatgcgggg aacaatctga tcgacaaaac
    16561 catgagataa taagaattca gccttttgga aatcttctgg aagttcctgc ttgatcgtct
    16621 gttcgatgac gcgtcttcca gcaaaaccga tcaagctttg gggttctgct agaataatat
    16681 cgccttccat tgcaaaactg gcagttactc ctccggtcgt cgggtccgtt aaaacagtta
    16741 gataaaaaag accttcattg ctatgccgtt tgacagcagc agagattttt gccatctgca
    16801 ttagagaaaa gattccttct tgcatccgtg cgccacctga agctgtgaac aagacgactg
    16861 gaagactttc ctttgttgct cgttcgaata atctggtgat cttttctccg acaatcgttc
    16921 ccatacttcc catgataaaa ttaggatcca tcactccaat aatgaaggga atcccctgaa
    16981 tggttgcttt accagtgagg actgcttcat gaagtcctgt tttatcttgc atttttttaa
    17041 ttttttccgt atattcggga aatgctagcg gatctttggt tacaagatcc gtatcccatt
    17101 cttcaaaact tttttcatca ataatcaaag ctagacgctg ccatgcaccg attcgaaaag
    17161 cgtatccgca atgagggcat actttttctt caccgatgtc tttcgtataa aggatatgtt
    17221 tgcaatttgg acactttgcc cacatgttat caggaacaga tggcgagtta gcaggtttgc
    17281 tctctctatt aggattgatc cgaatatatt tctttttctt aaacagagcc atctatgcgc
    17341 ctcctatctc cgtttctggt gtccaattcg gtaaaaattc ttcttgtaaa aatgctgtgc
    17401 tgtaatcacc tgctatgact gcaggatgac taatcaaatc catctgaaat tctgcgttcg
    17461 tgatgattcc ttctgtgata aattcactta aggctcgctg catcttcatt aatgcttcaa
    17521 aacgcgtatt tccatggacg attatttttg caatcattga atcataatat ggcgggatcg
    17581 tatatcccgg gtaaactgca ctgtctactc gcagtcccat cccacctgct ggaagcagta
    17641 agttttggat cttccctgga gaaggagcaa aatggaaagc aggattttct gcattaatcc
    17701 ggcattcgat tgcgtgtcct tggaatacta cttcttcttg acacacattc aaaggctcac
    17761 cggctgctat ctccacctgt ttttttacta aatcgattcc tgtgaccatt tctgttactg
    17821 gatgttccac ttggattcga gtattcattt ccatgaaata aaaatcgcct tcttcatcca
    17881 tcagaaactc gatcgttccg gcattttcat aattgactgc tttagctgct cgaacagcag
    17941 cgtctcctag tgcttctctt tttgtttgtg agatcactac agaaggagat tcttccagta
    18001 ctttttgatt gttgcgttgt aaagaacaat ctcgctcgcc taaatgaatg acatgaccaa
    18061 atcgatcacc taagatctga acttctatat ggcgagcagg ataaatgatt ttttccaaat
    18121 acatatcatc atttccaaat gccgctgctg cttcttgctg agctgaagaa aaatgctgtg
    18181 ggagctcttc ttttgattga actttccgaa tgcctttccc gccaccacct gcagctgctt
    18241 tcagcatcac tggataacca attctgtctg ctatttccaa tgcttcttct actgtagata
    18301 atacaccagt acttcctgga atgactggga catttgcttc ttgcatcaat tgacgcgcat
    18361 tgatcttatt tcccattgca tcgatcgttg cacttttagg accaataaat gtgatattgc
    18421 actcttcaca catggaggca aatcggctat tttcagataa aaaaccaaat cctggatgaa
    18481 ttgcttcggc ttttgtcacg attgcagcgc tcagtacttc ctgaacattc agatatgaat
    18541 caacggcttt tgccgggcca atgcagattg cttcatctgc taactgtgta tgaagtgctt
    18601 cggcatctgc ttctgaatat atcgctacgg tttggatccc tagttcgcgg catgcacgaa
    18661 tgatacgtac agcgatttca ccgcggttag ctatcaatac tttagaaaac atatttcacc
    18721 tatccaatca taaaggttaa ttccgcttcg gctacttttt taccgtctac tttagcgatt
    18781 cctttgccta ttccggctga agctttcact ttcaatatct caacttcaag gtataaggta
    18841 tctcccggtg taactttctt acggaatttt gctttgtcca atccgccgaa ataagccgtc
    18901 tttcccttga attgttcaag agaaagtaat gcaactgctc ctgcttgagc cattgcttca
    18961 acaatcaata cccctggcat aacaggttct tctggaaaat gtccttgaaa aaagggctca
    19021 ttgatcgtta catttttctt agcaacgatc cgttctcctt caaccatttc ttccactcga
    19081 tcaatcaaaa gcattggata acgatgtgga atgatttctt ttatttcttg tatgttcatt
    19141 gttttccctc tttcctgctt tgcttatttt tgctgaatcc taaagagtgg ctgtccatat
    19201 tcgacaactg actcgttttc tacaaggatt tctgtgatca ctccgtcggc ctcactagtt
    19261 atctcattca tcagtttcat cgcttcaata atgcagataa cttctccttt ggcaacttga
    19321 tcacctactt ttttgaaagc tggtttgtcg ggcgctggtt gcagataaac gattcccacg
    19381 attggcgaaa caacctcgat tccatcaaat actgccgatt ctttttcttt cattatttct
    19441 ggagcagttg ttgcttgttc aggtatgaca ttgactggct ccgaaactgg caacgtttcg
    19501 tttgtttgaa ccatattcat gcctcgactt gttttatttt tattcatata caattcaaat
    19561 tgaccttcac gcaaatcgaa ttccgttagc gaggattgat caaattgact gactaattct
    19621 ctgatttcat tgatatccaa aattattcct cccagcgttt catgcacaga accgcattat
    19681 gtccgccaaa ccctaatgaa ttactcattg cataagtaaa acttgcaggt ttgctttcgt
    19741 ttttgacaac tgtcaagtca atctcaggat cttgtttttc tacattgatc gtaggtggga
    19801 taaattgatg ttctaatgcc agcaaagttg ccaaggcttc gatcccgccg gctgctccca
    19861 ataaatgtcc agtcatcgat ttcgtgctgc tgacgtaagt atctctgaaa ttttcaccta
    19921 atgcgatttg gattgctttt gcttctgcct ggtcattggc tggtgtactt gttccgtgag
    19981 cattgatata tccgacttgt tccggctgta caccagcttc ttccatcgca agcttcattg
    20041 ctttagatgc accagaacca tctggagtag gagctgtcat atggtaagcg tcacagtttg
    20101 caccgtatcc aacgatttct ccatagattt tggcaccgcg agcttgtgca tgttctaaag
    20161 attcaagaac tagaacgcct gctccttcac ccatcacaaa accattccga ttttcgtcaa
    20221 acggaatcga accacgatta ggatcagttg attctgagag tgctgttaaa gctgcaaatc
    20281 cagcaatccc catctcacaa atcgttgctt ctgttcctcc agcaagaatg acatcagaat
    20341 agccatgttt gatgttacgg aatgcttccc caattgaatt cgttccgcta gcacaagctg
    20401 ttacgatagc tgtacaaata ccttttgcac ctacacgcaa agcaatattt ccagctgcca
    20461 tgttggcaat cgccattggt acgaacattg gagaaactcg ttttgcacct ttttcatgta
    20521 gacgaataac ttggttttcg attgttggaa gaccaccgat cccagatccg atgatgacgc
    20581 caaaccggtc ttgattcatt ttttctttat ctaaaccgct catctccatt gcttctaaag
    20641 cagcgtagat tccatagatc gaaaacatat ccattcgttt gctatctttt ttaataaaat
    20701 atttatcgta agggaaatct ttgacttcac ccgctaaggt aatccctgtt tctgaagcat
    20761 caaatttagt gatcggtgcg atgccattcg tcccctcttt taagctattc aaaaagctgt
    20821 ctgcgtcatt tccaataggt gaagtgactc catagccagt aattaccact cgattcattt
    20881 gtttgctctc cttttcttat ccgtgcatga ccaatccgcc atcgacattg atgacttgtc
    20941 cggtgatata tggattttgt gctaagaaaa ctgctgtttg tgcaatatct tctacttgtc
    21001 cgaaacgctg catcggaatt tgcttttccg cttgttcctt gactttgtct gctaaaactt
    21061 ccgtcatatc agttgtgatg aatcctggcg caatggcatt acatgtaatg cctctagttg
    21121 cagcttctcg tgccacagat tttgttaatc ctactacgcc agctttacta gctgcataat
    21181 ttgcttgacc aatatttcca atcaacccag atacactaga gagattaatg atggcccctt
    21241 ctcgctgctt catcattttt ttcaatacgt gctgcgtcat attgaaggtt ccaatcaaat
    21301 tgatatccag acattttttg aaatcttctg catccattcg catcacaagt ttgtcatttg
    21361 tgattccggc attgtttact aacacatgga tcgatccaag cttttcttct gcttctttga
    21421 tcatttgtcc cgctttctca taatcagaga tgtcaccgga aactcctacg cattttacac
    21481 cgaatgcttc gatttcttct atcttttctt ttggaatctc tccgcgacca ttcaaaatga
    21541 tatttgctcc agcttttgca aaagctaagg ccatcgcttt gccgattcct cttgtactac
    21601 ctgtgataaa tacatttttt ccttttacat ccataacgtt cctccttata gtattgctat
    21661 tgtttcatca aatgtctgct tattctcaac acgcagaact ggtattgttt tatcgatttt
    21721 tttcataaat ccgcttaata cttttccggg gccgacttcg atgacttgtt cgatgccgat
    21781 tgtttttaac ttatggatac tctcatagaa acgaacaggt tgcatgactt gctgttcaag
    21841 tagtcccgcg attgtttctt tcttcattat ttccgttgtt gtgttgctta tgataggaaa
    21901 agaaggttca gagaattgaa tctgacttag gtccttcgcc agtttttttg ctgccggttc
    21961 taaaatagct gtatgaaaag gcccgctgac atttagaggg atcattcttt tgaacccttt
    22021 ttcttttaat agtaaaacag cttcgtctac tgctttttct tcgccaccaa tcacgatctg
    22081 ctgaggtgta ttataatttg caggagatac gatgccgtac ttgctagctt catgacagct
    22141 ttcctcgatc gtttcaatcg gcgcattcat cactgcaacc attttcccgc ttccagctgg
    22201 ggcagcttct gtcatataag cgcctctttt tgctactaac gcaactgctt cagtgaagga
    22261 tagcgcatta ctagctacta atgccgaata ttctcctaaa ctcagtccta aagctgcatc
    22321 tgggatgatt ccatgctcct tcaataagcg ataatacgcg atgctgactg ttagaatagc
    22381 tggctgtgta tattgcgtct gatccaaacg ttcattttct gtaaagcaaa gttcagccat
    22441 atcgtagccg agaatctcac ttgcttcatc aaatgtctgc tttacaacgg cttcttcata
    22501 taaatctttt cccatacctt gatattgtgc accttggcca ctaaataaga atgctgtttt
    22561 catgtttttc tccaatatat ccagaatata tgtttgattc ttcctattca aaaaggccga
    22621 gacattccta tttcaggaaa tgtcccagcc cccaacctaa ttttaatgat tatgcagttt
    22681 gtttttctac gtaagtcact aaatcttgaa ccgttttgat tccgtcttct gtttcgattt
    22741 tcacgtcaaa ttcatcttca atttcattga tgatttggaa taaatccaag ctgtcagctt
    22801 ctaagtcttc ttggatgtca gtagtcaatt tgatttcttc agtttctttt cctaattctt
    22861 ctgcgatgat ttcttgaatt ttttcgaata ccataataat atattcctcc gatattgttt
    22921 gctttttatt tttataattt aacagtctac aactccatga gaatacttcc ccatgttaaa
    22981 cctccgccat agcctgtaaa tattactttc tgctgttttc ctaaggtgat ctttccacta
    23041 gaaactgctt catccaataa gatagggatg cttgcagcag atgtattccc atatttatcc
    23101 atattggtca ggaacttttc ttgcggcatc tttacttttc gggcaatttt ctcaataatt
    23161 ctgaggtttg cctgatgcaa aagcagataa tcaactggtt tgtctttcat gacttcttgg
    23221 atattgtttg caacgtctcg tacggcaaaa tcaaaaatat ctctgccagt catttgcaaa
    23281 taatacgaac cttctgaatc ttctgaatag aacggatttt tattttgctg gtagccagcg
    23341 gtcaatgagc tacttctggt accgtcagct gctaattttt ctttcagcaa ttttttctgt
    23401 ggattcgctt ctaagagaat cccacctgct ccatcgccaa acaacacagc agtcgtacga
    23461 tcagaccaat cgattagctt agatagtgtt tcaccaccaa tcaccagtcc cttagttgag
    23521 ccgcagcgga tcattttttc agccatgctt aacgcataaa caaatcctga gcacgcagca
    23581 ctgatatcaa aagcgatagc atgagttgct cccaatttcc cttgtacttg tgctgctaca
    23641 gatggtgtcg tgtaatcggg cgacattgtt gctactaaaa taaaatccaa atctttaggg
    23701 tttagatttt tctgttttat caatttttcc gccactaaaa tgcataaatc agaagtctcc
    23761 tgatttgtta cacatcttcg ttcacgaata cccgtccttg acgaaatcca ttcatcagag
    23821 gtatccatca tcttggaaag atcattatta gtaataatct tttcaggaag gacgctggct
    23881 gttgcagtaa tcattccaaa ttgttccatt aaatatcctc acttatagtc ttgcaagaag
    23941 tcgtgcaagt ttttcagtgc atccagcaag gcatgctgtt cctccgtagc cattccgtct
    24001 aagatgttct tgaccatttc acgatgaaaa tgctgatgga cccgaaatag taactttcct
    24061 tttttcgtta gtcccaactt tacaacgcgg cggtcatctt cactgcggat acgttcgaca
    24121 tagccttttt ttactaactt gttaatagca gtggtcaagg tcccaacagt aattgatagt
    24181 tctttggcta cttctgaagt tgtttttttc ttgtacatcc cgattgcctc gatcgtgtgc
    24241 atctctgtga ttgacagatc tttgaattga gattttttca gctcagactc ttcgattgtc
    24301 aaaatatcat tgaagacact aacaagataa tcatttactg tttttaaatc tggttccatg
    24361 ttccaccgtc cactaattag tttgattatc aaatcttttg atgttcaaac tatatcttaa
    24421 cttttttcaa aatgcaacct taaaagatca tttttttact atatgagctt tttttaagat
    24481 tttactcatt taaaacaact gataaatcag catttataat ttcttttatt agaaaaatca
    24541 tctatctcac atattttgat tatcaaagta ttttctgtga acagaactat agaattccct
    24601 aaaacttgtc cattatttca aaatttgtta tagtcttttt attcaaagcg taaaaggaga
    24661 tcctatgtta aaagaaaaaa tgaaaactct ccccatgctg ccgggtgtct atcttatgaa
    24721 agaccgagat ggtaaggtca tctatgttgg taaagcaaaa cggctcaaaa atagagtaag
    24781 cagctatttt catcagaata aacagcattc aaaaaaagta ttacgtatga ttcatcatat
    24841 aacagatttt gatttcgtcg tagtagacac ggaattagat gctttattgc tcgagtgtca
    24901 attgatccaa cattaccgtc ctttttataa ccgacaaatg aattatttca gtaattataa
    24961 ttatgtacat attaccaata agggatttgt attaacagat actcctacgg ctcgaacata
    25021 cgggcctttt cgtctctata aaaaaatgcc gtctattttg cgtataatgg aagaaactta
    25081 tcaaatgcct tggttgtctg aaattagttt gttagctttg cgagtccagc ttcctgacct
    25141 acaggaaatg acttttgaac agaaaaaaaa ggaactccaa gggctgtttc aaggccgaaa
    25201 taaaaagcta ctgacttact tgaaaaaaag acaacaacat ttcatctatc agttgaattt
    25261 cgaaaaagcc ggtatgcttc aaaaagatat cgaattagtt acttatttta ttagacggat
    25321 ccaggaacaa aagcagtttt taagaacacc atcgctcacc ttcagcatgc cattagcagc
    25381 agatgagagt cagaaaaagc actacctcgt ttgttatggt caacttgcag aaacaatgat
    25441 tacctctggt gacgtttctc ctgacttcta ttatgaaaag aaagaagctc atttatcttt
    25501 gaaacgtcaa ttgtcaaaag aagaaatcga tcctgtacaa atcttgatca gttaccgcaa
    25561 aaaattagaa aaagaacagt ttgaaattga actgttaaac aaaaaagaag ctgaaaagca
    25621 actaaattag ttgcttctcg gcttctttta ggcatcttca acttcaaatg tcaattatcc
    25681 taaaatttat tggattaagt catagatttc catcgctacg atatcgatat tatcaaattc
    25741 gtattgctga tgagtatctg tttcagttaa ttcaaatgta tcggtcgctt tatcataagt
    25801 tactgtacac ttctctacgc cttctttttc aaaacggcga acttgcagtt cattatctgc
    25861 tgactcgacc atagcctcca gtcgttttat gattgcgact aattgtgaat gtgtcatcgg
    25921 aggtcctcct tttcttattt ctgttatatt gtatcaaaag catagcctac ttgcattgat
    25981 tttctctaaa aaaactcgtt ttttatgatt ttttttctgg tatttttcct tcaggccacc
    26041 aaagtttgat caaagcttgg gttccgtcat tctcaaaacc tttatctgcc aactcttcat
    26101 atagtttcaa tgcttgggta gttgctggca atacaagatc cattttttct gcttcttcta
    26161 atgcaatctt taaatctttg ataaaatgtt tgacaaaaaa accaggtgtg taatcttctt
    26221 ttaaaatacg aggagaataa ttgcttaaag accagttagc tgcacttcca cctgaaagcg
    26281 tctcaatcac tttctttaaa tcaagtccgt ttttttgtgc atagacaagc atttccgtca
    26341 ttcctgtcat cgtacctgca atcatgatct gattagccat ttttgtatgc tgacctttcc
    26401 cagcagaacc atgcaatgta aatgtcgtac caaattcttt gaataaaggc agtacttgat
    26461 cataaacggc ttcttctcca ccgaccatga tcgtcagtgt accattcttt gctcctaagt
    26521 caccaccgga taccggtgca tctaacgcat ctgcgccttt ttcttttgct gtttttgcaa
    26581 ttttttctgc caggcttggt gtacttgtag tcaaatcaac taaaatatgt cctgagatat
    26641 ctgcagaaaa aatccccgac tgtccatagt agatttcttc tacatctttg gggtaaccta
    26701 ccattgtaaa gagaatatta ctttgttctg ctaccgcttt tggtgtatct gcccagatag
    26761 ctccttctgt tactaaagga tcagcttttg cttttgtccg attatagaca gctacttcgt
    26821 gtcctgcttc tagtagatgg cgtgcgacag cacttcccat gacgcctgtt ccgataaaac
    26881 caagtttcat tttttcgacc tcctaaaaat caaaaatcct tgcttctatg ctagtatacg
    26941 ctagaaacaa ggattttttc ataaaaaatg tttgaatttt atcttttaaa tgaatttaat
    27001 tttattttga ttcgttcaat cgagtcttct tcttcttttc ttcgcatctc ttcttcatga
    27061 taaacaacca tgtcttgata aaattcgatc atagcttcgc cgaaatgtgt agatgaaata
    27121 tcataaagtt tagcttctaa aacttttgga tctttaggaa cttcactgga aatataatca
    27181 ataagtgctg gtgcaaagtc ttcgtcggtt tcaaaagttt tccctaatga aggatgatcg
    27241 aacaatcggt tcaaataagc atttccttcc acgacacaag gtgttccggc tgccatcgct
    27301 tctgtataag taagcccttg tgtttctgaa gtagaagcac tgacaaaata atctgccgct
    27361 ttataataaa gtgctacatc attgttgtcc acttctcctg tgaactgcac aaattcttct
    27421 aactgcagtt ctgagatcaa ttcctctaac tcttctacat aaggtccttt tcctacgata
    27481 actaatctgg cgttaggata agcggagatg acctgaggaa acccattaat caaggcttgg
    27541 atatttttct cataagaaat cctgcttaaa gaaagaatca tcaaatggtt ttcctgaaga
    27601 cctagtgact cccttaattt gctgatatcc tgctgagtga tatcagaacg tttgaatttt
    27661 tcaatgtcga taccagtagg aatcacgcga agcggtgcta ttacaccata gctgcgtaat
    27721 gtatcaataa cacgttcact tggacaaact acacctgtcg tatgatttgc aaataatcta
    27781 gacaagtatt ttacatggga ttgcctgatc acttttcctt taccgatata atgaaggtag
    27841 tcttcataca tcgtatggta agtatggatc agaggtattt tcaacttctt agctaccatc
    27901 ttcccaagta tccctgctcc gaattctgta tgggtatgaa taagatctag ttctagttct
    27961 ttggcaacca aataagcata ccacatgcct ctaacaacaa ctcttcgatc cttgaaagaa
    28021 ataaatggta cacttggcat tcgaatcaca tctttttcca acttatccgc attaggatcg
    28081 gtcgttgtaa aaatatagac ttcatgccca tgtctttcta attcttgttt taatgttttg
    28141 atcgatgtag caactccgct gacttgcgga aagtaggtat cggtaaaaaa gccgattttc
    28201 atgtttttcg cctccaaact agtccttgac ctgatttact ttttttaatt tgctagagac
    28261 tttttcattc agtacacttt cgtatacatt tttaagttct tcaccgattt tattgatact
    28321 tcgctctttt gctgtttgat aaccggcttg tgtcgtactc ggtagttctt gattggtaat
    28381 ctgttggatc agtcggataa attcttcgtt tgtcttcccc atatagcagt tcttgctatc
    28441 ttctaaccag ccattataca ctggaatatc tcgaacaagt acgttttgtt tgcttgctaa
    28501 agcttctaaa acaacgatcc cttctgtctc ttcataagaa gggaaaaaga atagatctgc
    28561 acctgaatac gcaccttcta tgatctctcc acgaatgtaa ccaggaaaca ggacattttc
    28621 aggatgatct tcttttacaa ttttccggat gttccgagga atcgagtaca tgggaacatg
    28681 accaaaccaa ataaatgtgt attcgggcat cttcttagca atttctacga aatcaatgat
    28741 tcctttacgt tcgaaaaaca aaccgacaca aatgattact ttttgatccg gagacagttt
    28801 gaaatattct ctgaactttt gttctttcat tggatcaggg tgatatttct ctaagtcgat
    28861 cccattggaa atagcttgaa tcggcacacg gataccatag ctttccaaca actgttttga
    28921 ataaggtgtt ggcgtgatca gatagtccgc tttttggtac aatcctacca gatatttttt
    28981 tacgatagga gaaagctggt tcgatccgat aaaggaattt cggaaatctt cctctgtaga
    29041 atgcgcatga tagacaactt tttttccttc tcttctggct ttattgacca tattatgact
    29101 attgattccg tatgtattga tgtgcaggat gtcataatct tctttttcat ctagtgtata
    29161 agagataccg acttccgtca gagcacgttt ttgatgatca agcgcacgcc cgattccaga
    29221 ttttgcaagc atcttttcac tttcaaaata taataatacc ttcaccttac gtcccccttc
    29281 acttatatag tataacatag cctacaaact gtcactcaaa cttaagccct atttttgtct
    29341 attcttactg acaaaaacgt tttcttaaaa aagaacaaaa aaactggcgt agctctttta
    29401 caagccacac cagcttttga tacgttgttt attttacata ttcatgaaca agttcaacga
    29461 cttcttccat cgtatcgcat tctttcaatg cacgatcagc aagttcagcc attttcgctg
    29521 tgtccaagcg tttcattaaa ccacgcgttt taagaataga tgtcgcactc attgagaact
    29581 catctagacc catacctact agcaatggaa cagctgtttg atctccagcc atttctccac
    29641 acatacctgc ccattttcct tcagcgtgtg ctgcatcaat tacatttttg atcaaacgta
    29701 aaattgatgg gttatatggt tggtacaagt aagaaacgcg ttcgttcata cggtctgctg
    29761 ccattgtgta ttgaatcaaa tcgttcgtac ctacagagaa gaaatctact tcttttgcaa
    29821 acttgtctgc taagactgct gctgctggaa tctcgatcat gatgccgact tggatcgtat
    29881 cagatacttc cttgccttca cttatcaatt tttgtttttc ttcttcaaag atcgcttttg
    29941 ctgcgcggaa ttctttgagt gtagctacca tagggaacat gatacgtaaa ttaccatgaa
    30001 cagatgcacg aagcaatgca cgcatttgtg tacggaacat ttcgtcgcct tgttcagata
    30061 aactgatacg caatgcacga tatcctaaga atggattcat ttcatgaggt aattgcagat
    30121 atggtagttc tttatctcca ccgatgtcca ttgtacgaac aactacaggt tttccttcca
    30181 taccttctaa tacagctttg taagcttcat attggtcatc ttctgttggg aagtctggag
    30241 aatccatata caggaattca gtacggtaca gaccaactgc ttctccacca ttattatgga
    30301 caccaactag atcttttggt gttccgatgt ttgcagctaa ttcaaaatgc ttgccgtcag
    30361 ctgtgacagt ttctgcattt tttaattttt cccattctgc tttttgtgct gcgtaatctg
    30421 cacctgcttt ttcaaattca gctttttgtt catctgtagg atcgatgatt acatcgcctt
    30481 cgatcccgtt gacagccaaa atatcgcctt ctttgacttt agcagtaatt tcttttgtcc
    30541 caacgattgc tggaatttct aaagaacgtg ccatgatagc agaatgtgaa gtacgtccac
    30601 caatgtctgt tacaaacgct tttacaaaat tgcgatctaa ttgtgctgta tcacttggag
    30661 tcaagtcatg tgcaacaacg accacttctt cgttgatcat tgatggattt ggtaaagtaa
    30721 cgcctaataa gtgcgctaaa atacgtttag caacgtcacg aatatctgca gcacgctctt
    30781 gcatataagc attatcttcc attgcttcga acataccgat atacatatcc gttacttctt
    30841 ttagcgctga ttcagcattt acgctgttgt ctttaatatt ttgcttgatt tgacctacca
    30901 tttctggatc tgaaagaacc atcaaatggg catcaaatac ttgcgcttct gcttcaccta
    30961 agctttgcgc tgctttttca cgaatttgct gcaattcttc agtagattta gctaatgcag
    31021 catccaaacg agcttcttca gccgcagtat cttctactgt tgccttactg aatgttaaat
    31081 ccggttgaac tagcaggtaa gcttttgcaa cggctacacc gtcactagcg gcaatccctt
    31141 ttagcatttc aaccattatt cagataagcc ttctttcttc attgtttcaa cgattgctgc
    31201 cattgcatca gcttcgtcag caccttctgc agtaatagta acatcagaac cttgacctac
    31261 acctaaagac ataacgccca tgattgattt caagtttact gatttacctt tatactctaa
    31321 gtttacatca gagttaaatt tgcttgctgt ttgtactaat agtgtagctg gacgagcgtg
    31381 gatcccagtt tctgctacta cgtgaaattc tttcttttcc atattaatcg gtctccttta
    31441 agtgagtgtt gtttttatgt gagggttttc ttttttagta attcagagaa tcctaacaaa
    31501 tgccaccctt tcatctgtaa agattaacat tttttttcgt tagatacaac agttttcatt
    31561 caatttcaga taaaagtatt caaattattc cggattcttt ttcatccttt tcttcacatt
    31621 ttataacaaa cgtgtttatt tcctttttct tgtcgctttc tcttctcctg acgtttatca
    31681 ttctttcctt tgaccctctt tgttttcata tgaaacgaat tacttgcttc cctttttatt
    31741 tttagtataa taagagtcaa aggtcaggaa tggtcaatag agatttcacc ttttctacct
    31801 cataaatctc ttaaaaaact aggggttatt cttttacctc atgaaaaacg agatgtaaaa
    31861 tatggatgta cccatttttg tgaaaggacg tgaatgattc atgctttgtc aaaactgcgg
    31921 caagaatgag gcaacgattc acttgtatgc cactgtcaat ggtcagcgga cacaactcga
    31981 ttactgtcag agctgttatc aaaaaatcaa aaatcaacaa aatggaggtc tgactaatat
    32041 ggcccaaaat gatccatttg gatttggcag tttggacgat cttttccgtt ccatgtctcg
    32101 ccaaatgcaa cagcaaggaa attatgagca aacaccacca acacaatttg gcggaaacaa
    32161 caacttcaat ggtggtcagc cacctcaagg caatacagaa ggtttattgg gagaatacgg
    32221 aatcaatatc acagaacaag cacgacaagg tgatatcgat cctgtgatcg gacgcgatga
    32281 cgaaatcaaa cgagtcattg aaattttaaa tcgccgaacc aagaacaatc ctgtcttgat
    32341 tggagaacca ggtgttggta aaacagcagt tgttgaaggt ttagcacaaa aaatcgtaga
    32401 tggtgatgta ccgcaaaaat taatggacaa agaagtcatt cgtttagatg tcgtatcttt
    32461 agtccaaggt actggtatac gaggacaatt tgaagaacgt atgcaaaaat tgattgaaga
    32521 gatccgtcaa gcagaaaatg tgatcctatt catagatgaa gttcacgaaa tagtcggtgc
    32581 tggttctgct ggtgatggaa atatggatgc tggtaatatt ttaaaaccag ctttagctcg
    32641 aggagaattg caaatggttg gtgctactac cttaaatgag taccgcatca tcgaaaaaga
    32701 cgctgcttta gaacgtcgta tgcaacctgt acgggtagat gaaccaactg tcgaagaaac
    32761 gatcagtatt ttgaaaggtt tgcaaaaacg ttatgaagac tatcatcatg taaaatatac
    32821 tgatgaggcc atcgaagcag ccgcaacttt atccaatcgt tacgtccaag atcgcttctt
    32881 gccggataaa gccatcgatt tgctagatga aacaggttct aagaaaaact tgacgatcca
    32941 aatcgttgat cctaaaacaa tcgagaaaaa actgcaagaa gcagaagaac aaaaagtttt
    33001 agcttctcga gaagaagatt tcgaaaaagc agcttattat cgcgatcaga tcaataaact
    33061 tcaaaaaatg aaagaacgtc agctcactga agaggaaaca cctgtgatta ccgaaaaaga
    33121 tatggaaaaa atcgttgaac aacggacagg tattccagtg ggtgaattaa aagaaaaaga
    33181 acagacacaa ctgaaaaact tagcagatga cttgaaagct catgtcatcg gacaagataa
    33241 cgctgtagat cgagtagcga aagcgattcg ccgaaaccgt gttggattaa acaaacagaa
    33301 tcgtccaatc ggctccttct tatttgttgg accaactggt gtaggtaaaa cagagcttgc
    33361 gaaacaactt gcctacgaac tattcggttc acaagattca atgattcgtt tcgatatgtc
    33421 tgaatatatg gaaaaacata gtgtctctaa attgattggt tctcctccag gttatgtcgg
    33481 atacgaagaa gctggtcaat taacagaaaa agtacggaga aatccttaca gtcttgtctt
    33541 gttagatgaa gtagaaaaag ctcatccaga tgttttgcat atgttcttgc aaatcttaga
    33601 tgatggtcga ttgacagatg cacaaggaag aacggtcagc tttaaagata cgatcatcat
    33661 tatgacaagt aatgccggaa ctggtaaagt agaagcaaac gttggttttg gtgcggcacg
    33721 cgaaggtgtt actcgttctg tattgaacca attaaacaac tactttacac cagaatttct
    33781 gaaccgtttt gacgggatca tcgaattcag tgcattatca aaagaaaact tgatgacaat
    33841 cgtcacacta atgctagacg atgtcaacca aatgctggca gcacagcaac ttcacatcga
    33901 agttcctacg aatgtcaaag aaaaattagt tgacttaggc tatgatccat ctatgggtgc
    33961 tcgtcctctt cgacgcacga tccaagaaca aatcgaagat ggtatcgctg aattttatct
    34021 tgatcaccct tctatccatg agttgaaagc aaaactggat aaagatggaa aaatcatcgt
    34081 tacttctaaa ccagaacgtt tggctaaaga atcagccgaa gaaacggctg aataaatcca
    34141 gttttatagc gtgagacggt atttctttga gaaatgccgt ctcattcttt tttcagaaaa
    34201 gtcaaaatac tgttcatttt ataaaaaaga aattataata agagaaacaa gcaattaaaa
    34261 atacataaaa gaggtgagcg acatgaaatt ggtaaatgta acaaacagcc attcccgtct
    34321 tgtattaaac cagcttgaaa acactgatgc ccatctagtc aaggtctaca cagccggaaa
    34381 taccactatt gtctacactg aagcccctca acataatgaa attctgctga tcaatgacaa
    34441 acgtaaaatc cagccgaaag aaatcgaaga tgctaaaaat tatttcttag ataaaatcaa
    34501 agacgccgtt taccacgagg atgaaatcaa agtaatcgaa ttggcaggcc ttgttgaaat
    34561 ctcgattcca aaacatgaat taggcgctac attagccgtg taaacaaatt ttgaacataa
    34621 taattaaaga gcgtgggaca aaagtaaag
//
LOCUS       DAZKKW010000022        34624 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.022, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000022 DAZKKW010000000
VERSION     DAZKKW010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34624
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.022"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..86
                     /locus_tag="ACG8PO_001045"
     CDS             <1..86
                     /locus_tag="ACG8PO_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HFQ0206389.1"
                     /translation="QFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            58..405
                     /locus_tag="ACG8PO_001046"
     CDS             58..405
                     /locus_tag="ACG8PO_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0206390.1"
                     /translation="MVAIQKELNKTGFVLLESLTALMIASVCVYLVSFGQLFLLQAEK
                     KSGEELLFIRMIYEDIKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAI
                     SIEKTSNSSIYAD"
     gene            368..793
                     /gene="comGF"
                     /locus_tag="ACG8PO_001047"
     CDS             368..793
                     /gene="comGF"
                     /locus_tag="ACG8PO_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGF"
                     /protein_id="HFQ0206391.1"
                     /translation="MKKQVIPAFTLIECLIGLMILSSLLLTFNLLIQQTIQVSEFLQR
                     KDQKEWLIFLAQLEEELKSSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQ
                     PMLTSITELQLLEKEHTISFYVHFENGEDGYAIWTKNDQ"
     gene            765..1124
                     /gene="comGG"
                     /locus_tag="ACG8PO_001048"
     CDS             765..1124
                     /gene="comGG"
                     /locus_tag="ACG8PO_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGG"
                     /protein_id="HFQ0206392.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            1296..2303
                     /locus_tag="ACG8PO_001049"
     CDS             1296..2303
                     /locus_tag="ACG8PO_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFQ0206393.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            2328..3512
                     /locus_tag="ACG8PO_001050"
     CDS             2328..3512
                     /locus_tag="ACG8PO_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HFQ0206394.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(3949..5532)
                     /gene="abc-f"
                     /locus_tag="ACG8PO_001051"
     CDS             complement(3949..5532)
                     /gene="abc-f"
                     /locus_tag="ACG8PO_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protection-like ABC-F family protein"
                     /protein_id="HFQ0206395.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(5637..5765)
                     /locus_tag="ACG8PO_001052"
     CDS             complement(5637..5765)
                     /locus_tag="ACG8PO_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0206396.1"
                     /translation="MMRMTKITSNENYQRYHIAFSHAIGFTVAVDGTSLSKTVEFT"
     gene            complement(6110..6253)
                     /locus_tag="ACG8PO_001053"
     CDS             complement(6110..6253)
                     /locus_tag="ACG8PO_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HFQ0206397.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(6475..6954)
                     /locus_tag="ACG8PO_001054"
     CDS             complement(6475..6954)
                     /locus_tag="ACG8PO_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFQ0206398.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(7027..7311)
                     /locus_tag="ACG8PO_001055"
     CDS             complement(7027..7311)
                     /locus_tag="ACG8PO_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0206399.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(7389..7583)
                     /gene="ssrS"
                     /locus_tag="ACG8PO_001056"
     ncRNA           complement(7389..7583)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACG8PO_001056"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(7655..8941)
                     /locus_tag="ACG8PO_001057"
     CDS             complement(7655..8941)
                     /locus_tag="ACG8PO_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HFQ0206400.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            9079..9750
                     /locus_tag="ACG8PO_001058"
     CDS             9079..9750
                     /locus_tag="ACG8PO_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HFQ0206401.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            9716..10204
                     /locus_tag="ACG8PO_001059"
     CDS             9716..10204
                     /locus_tag="ACG8PO_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HFQ0206402.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            10217..11164
                     /gene="prmA"
                     /locus_tag="ACG8PO_001060"
     CDS             10217..11164
                     /gene="prmA"
                     /locus_tag="ACG8PO_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFQ0206403.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            11165..11929
                     /locus_tag="ACG8PO_001061"
     CDS             11165..11929
                     /locus_tag="ACG8PO_001061"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFQ0206404.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            12162..14375
                     /locus_tag="ACG8PO_001062"
     CDS             12162..14375
                     /locus_tag="ACG8PO_001062"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HFQ0206405.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRQHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            14386..14832
                     /gene="dtd"
                     /locus_tag="ACG8PO_001063"
     CDS             14386..14832
                     /gene="dtd"
                     /locus_tag="ACG8PO_001063"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFQ0206406.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            15003..16262
                     /locus_tag="ACG8PO_001064"
     CDS             15003..16262
                     /locus_tag="ACG8PO_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HFQ0206407.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(16351..17298)
                     /locus_tag="ACG8PO_001065"
     CDS             complement(16351..17298)
                     /locus_tag="ACG8PO_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFQ0206408.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            17692..18984
                     /gene="hisS"
                     /locus_tag="ACG8PO_001066"
     CDS             17692..18984
                     /gene="hisS"
                     /locus_tag="ACG8PO_001066"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFQ0206409.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            18999..20768
                     /gene="aspS"
                     /locus_tag="ACG8PO_001067"
     CDS             18999..20768
                     /gene="aspS"
                     /locus_tag="ACG8PO_001067"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFQ0206410.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            20877..21752
                     /locus_tag="ACG8PO_001068"
     CDS             20877..21752
                     /locus_tag="ACG8PO_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFQ0206411.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            22018..23901
                     /locus_tag="ACG8PO_001069"
     CDS             22018..23901
                     /locus_tag="ACG8PO_001069"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFQ0206412.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(23976..24260)
                     /locus_tag="ACG8PO_001070"
     CDS             complement(23976..24260)
                     /locus_tag="ACG8PO_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFQ0206413.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(24250..24984)
                     /locus_tag="ACG8PO_001071"
     CDS             complement(24250..24984)
                     /locus_tag="ACG8PO_001071"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016430 - tRNA
                     (adenine-N6)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HFQ0206414.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            25113..25736
                     /locus_tag="ACG8PO_001072"
     CDS             25113..25736
                     /locus_tag="ACG8PO_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HFQ0206415.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            25928..27049
                     /locus_tag="ACG8PO_001073"
     CDS             25928..27049
                     /locus_tag="ACG8PO_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HFQ0206416.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            27056..30184
                     /locus_tag="ACG8PO_001074"
     CDS             27056..30184
                     /locus_tag="ACG8PO_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0206417.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            30203..33397
                     /locus_tag="ACG8PO_001075"
     CDS             30203..33397
                     /locus_tag="ACG8PO_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HFQ0206418.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(33543..34250)
                     /locus_tag="ACG8PO_001076"
     CDS             complement(33543..34250)
                     /locus_tag="ACG8PO_001076"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFQ0206419.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT        11354 a         6194 c         7332 g         9744 t
ORIGIN      
        1 atcaatttta ttgggaagaa aaagagcgga cgattacgta tcaattccag atagggagtg
       61 gtcgctatac aaaaagaatt gaataaaaca ggatttgttt tgttggagtc ccttacagcg
      121 cttatgatcg cttctgtctg tgtctactta gtgagctttg gccagctgtt tttgcttcag
      181 gcagaaaaga aaagtgggga ggaactgcta tttattcgaa tgatttatga agatatcaag
      241 acgcagcgga tgtatcacaa aagaccagag cgaagggaag aaatgatccc atacgatatc
      301 caaattgtcg ataataatgg agtaatcgaa aaagtgtttg tcaatatgga gaaagaagcg
      361 atcagtattg aaaaaacaag taattccagc atttacgctg attgaatgtc taattggact
      421 gatgatattg agcagtctat tgctcacatt caatcttttg attcagcaaa ccatccaagt
      481 aagtgaattt ttgcagcgta aagatcaaaa agagtggcta atatttttag cacaattaga
      541 agaagaactc aagagcagtc acaatgtctc tgtgaaaaat cagcaactgc actacacgat
      601 cgaggataaa cagtatatca tcgaaagata tcaggcaatg atacgtaaaa gaagaacttc
      661 cggcggacat caacctatgt taacaagtat cactgagctt caattattgg aaaaagaaca
      721 cacaatttct ttttatgtac actttgaaaa tggagaagat ggatatgcca tatggacaaa
      781 aaacgatcag tagaaaatgt tataagggct ctattttatt ttctgtcttg cttttatttc
      841 ttttatgtac gtttttattg atggcagcat tagaaagcta ccgtttgtca atggatctta
      901 atatacgaat caaaaattat tatatagcaa aaataatgaa gcagatggct ttatcggaac
      961 agtctacgct tgccgaaagt tcggaaggag ttttttacta cacaactgga tctgtaacat
     1021 atcagtgggt acaacagtcc ctattcttag aggtggaagt ctcaccgttt atctttcggt
     1081 tcacagaaga agtaaaaaat gatacagata ctagtacaga atgatggaat gttcgtgaaa
     1141 acgccctctt ttctgttata acactgtttt taataaaaaa atgtttgccg tttcacaaat
     1201 aaaaaacagt gaaaacgatt gaaattttgt caattgttcg atagaattaa acaggtgaaa
     1261 aaattgttat aatatatcta gaaatgaggt gcaccatgtt accagaaaaa atggaacaag
     1321 ggtttaatca aaacctggaa gctattcagc ttttacagaa tgcattggga acttcctttt
     1381 tagaagccta tgtcgaaaat gcagaaaatt taatagatga ttatcaagta cgtgtggtgg
     1441 atggagtgcc gacaaaagag acaacgcaac ggatcacagc tctttatgaa gagttgaaga
     1501 aactttcttt cgagccagaa gaatggcgca gactgtctca attactttta ttgaagggca
     1561 gccagacaga acatttgcag gcgaaccatc aactcactcc tgatagtatc ggttttttat
     1621 ttgtttttct tattgaacaa ctatacacaa ataaaaaagc accggttaag attctggata
     1681 ttgcggcagg aatgggcaac ttgttattaa cagttctttt aaacctatcg aatgcggggt
     1741 accaaacaga aggaattggt gtggatattg atgatacatt gttggctgtc gcagcttcta
     1801 caagtgattt gacacaagca aatgttcaat actttcatca agacgggttg caggaattat
     1861 taatagatcc tgtcgatttt gcaatcagcg atttgccaat cggttattat ccgaatgacg
     1921 aaaaagcaaa agagttttta acaagtaccg aagaaggcca tagttatgca catcatttat
     1981 tgctagagca atcaatgaaa tatgtgaagc cggatggttt tggtttgttt ttgatgccga
     2041 gtggattttt agaaacggat caaagtgaag aaatcaaaaa atggtttaaa gaagaaggtt
     2101 atcttcaagg aatgattcaa ttgccagatg aattgttccg taataaacaa tcgcaaaaaa
     2161 gtattttgat cttacagaaa aaaggaccac aagcacaaca agtaaaagaa gtcttgcttg
     2221 tgaaacttgc ttcattaaaa gaaccagaga aggtgacgga atttttcaac gagtttaaaa
     2281 actggaaatc atctgctctg taatcattca acgagaagga gagtcatatg tctaaaacaa
     2341 ttgcaatcaa tgctgggagt tcaagtttga aatggcaatt atatcaaatg ccagaagaaa
     2401 ccgtagttgc gaaagggatc gtcgaacgta tcggtttaca agattctatt tttaccatca
     2461 agtacggcga taatcaaaaa ttcgaacaaa tcatggatat cgaaaaccac gaagtagcag
     2521 taaaaatgct gttagatcag ttgattgaac tgcaaatttt aagctcttat gatgaaatca
     2581 ccggagtagg gcaccgtgtt gttcacggag gagaaactta tggggattca gtagtgatcg
     2641 atgagaaagt aatggaccgc attgctgaat tagcagaatt tgctcctttg cataatcctg
     2701 caaacttaat ggggatcaaa gcgtttaaaa agattttacc tgacattttg agtgtggctg
     2761 tattcgatac atctttccac tcaacaatgc caaagcaaaa ttatttatat agcttaccgg
     2821 tgtcttatta tgaagatttc aaagtaagaa aatatggctt ccatggcaca agtcatcgtt
     2881 atgtgtcgga acgtgcggca aaattattga acaaaccgat cgaagaacta aaaatcatca
     2941 cttgccactt aggaaatggt gtatcgatca cagcagtcga tggaggtaaa tccatggata
     3001 cttctatggg attcacacca ttagctgggg tgacaatggg tacacgttca ggtgatatcg
     3061 atccagctgt tctcccatat ttaatggaaa aattagagat tgatattgat gaaatgatca
     3121 atgttttgaa caagaaatct ggtctgctag gcctaacagg attgtctagt gatatgcgtg
     3181 atcttgaaaa gaactatgaa aaagaacata ttcgtttagc ttatgacatt ttcgtggacc
     3241 gcatccgaaa atacatcggc ggttatgtga caacgatgaa tggtgttgat gcaattgtct
     3301 ttactgctgg tatcggcgag aatgacggac atgtacgtag tgaaatcatc aaaggtatgt
     3361 cttggtttgg ttgtgaaatc gatccagcgt tgaatgaatt gcgtggtgaa gaagtagata
     3421 tctctacaaa agaatcaaaa gtaagagtat tagtgatccc aacagatgag gaactaatga
     3481 ttgcccgtga tgtggagcgt ctgcgcggct aagccaaagc attcgttagg ataaaagatg
     3541 aaacgcatgc aagagggaat tcttgcatgt gttttttttg cggagaagac cgcaaaatgg
     3601 taatttaact tcaatttccc ttctttttta caggatcttc ttagggaatt ttgcttggaa
     3661 aagtggtcaa aagggaaaag actggcattt tatttattga ttttcaggaa aatcccaata
     3721 ttttattttc attcatcttt ataagggaca ttacatatgt aaaaagaaaa aacattttta
     3781 gatcagcctc gtgggctgtc gactttattc ttcgctttat gtttagctag aaaaccgatc
     3841 cgaaacattc attgatcgaa taataaaaac ttgattttcc acctgtactt tgcctgaatc
     3901 gtgaaagcac aggtatgaaa atcaagtttt ttgttgcgaa aaaacgtcct agtcaatcag
     3961 ttggccgagc ggctggcggc tgatctgctc ctgatcaata acgagtgctt cttggaagtg
     4021 ttgctgcaaa aagtagcggt catggctgac tgctaggagc gttccttgat agtcttgcag
     4081 catttcttca atttcttctc gagcatagct gtcgaggtga ttggttggtt catccaaaat
     4141 caggacgttg attttttcta aaaagagttt cagcaacgcc aaacggattt tttcaccacc
     4201 ggataaatct ttgatccgtt tacccacatc gctttgataa aagccacttt tggctaattg
     4261 ccgtcgagcg acttcttcgt ttccagtcaa tgtgatggcg taagccagca aacggctatc
     4321 gggatcggca aactgcagct gctgcggcaa ataaccgatt tgcaagctac tacctaattt
     4381 gatggtgcca ccgtaaggct tttcctcacc gagtaataat ttgagtaagg ttgattttcc
     4441 tgcaccattg tcgccgatga tggctaaatg atctccacga tagactgcaa aactgctgtc
     4501 tttgaatagt acttgatcag cgtactgctg gtgcaagttt tgaatgatga aaacttcttt
     4561 gccagatttt ccaccattag acaagctttg cagccgattt ttcgggggag gtggcggttt
     4621 gacgagtgtc agtttcgcca ggcggcgttc cagttctttg gcttttttga agaaggactc
     4681 attgtctcct tcatgtgccc actgacgaaa acgacgaatc atgactttca accgctggac
     4741 ttctttttgc tgcagttcgt aattttttgt cagctcagcc aagcgagctt ctttcaagac
     4801 tcgaaaacgg ctgtagtttc cgggatactc aatcaatgca ccttcttcca gttcccatgt
     4861 ttttgtggtg acttgatcga gaaattgccg atcatgagaa acgaccagaa ccgatttttt
     4921 tgtattctgt aaatagtttt ccaaccagcg aatactgtta agatctaaat gattggtcgg
     4981 ttcatcgagt aaaagtaaat cggcatcggc tgctaaaatt ttggctaaag cgactttaac
     5041 tcgttcgcct ccgctaagtt ctgttaaccg tgcttcctga cggtgtggaa tccctaagcc
     5101 ttgcaagatc gctgcaatcc ggtcttctaa tacgtaaccg cctagttctt caaagcgatg
     5161 ctgcagctca ccgtaacggg ccagacactt ctctaagttt gtatctggtt ctgtcatcaa
     5221 tgcttcgtag cgactcattt gctgtttgag gtcctgtatc tcagcggcag aatggagcaa
     5281 gtagtggttg acggtcgctt cggaaactgt aagttcttgt tcgaccgtcc cgatctttaa
     5341 gccttttttg cgactgataa caccgctgtc gacaccttct ctgccggtca agatttgtaa
     5401 aaaggttgat ttgccagttc cgttgatacc gactaaggca attttttcac cagcttcaat
     5461 ttgacaggat aaatcctcaa ataatggatt cccgccgatt gtttttttaa tagaaattgc
     5521 ttgtatgatc atgatctctc tccttgagac cactcaaaaa ggccatggtt atccctggcc
     5581 tgtgttattt aaactcagat tatcatgaga aaaatacctt gagatggcca ttgattttac
     5641 gtgaattcaa ctgttttgga caaacttgtc ccgtcaacag caactgtaaa accaattgca
     5701 tggctaaatg cgatatggta tcgttggtaa ttttcattac ttgttatttt agtcatgcgc
     5761 atcatagtat tttccctcct tcgtgtagtt gtcttttcca gtgtacctga aagtgtcaga
     5821 aagttcaact agcgctgaca tttacttaat atttatcgat gtgtaattct gtcagagagc
     5881 tttttttatc tgactgaggg taatgtctcg tggaccacat cattgtattc tgagctaaac
     5941 cagcgttcag gcgaaatcat atggtgtttg ataaaaaact ttgtctacat gacttccttt
     6001 tgccatttgc ttaataccgt tgcattaaac agctcttctc tttggaaatc tcaccaacat
     6061 cgcgttcagg agtatcgttg tggttgcaat ggcaagcggt agcctttcat caattcttga
     6121 atgatttacc gaatgtagca cttgccttct cgcggtaccc gaacccgaaa taaaatttta
     6181 gtttggcgat aaaatcggta tccgccatcg atacttcata ttttttcgta tcagattcat
     6241 cggaaacttc cacattgacg aaccaataag ctttggcatg cttaggtcgt tttaaaccac
     6301 accagggcga ctaatcgccc tggtgttcac tttttttatt ttacttgaac tcattgactg
     6361 ttttcccttt attttaaaca ttataaagac cgatgagtgc ttgccgcgtc ttttgttttt
     6421 atcacggttt tttgtgcaaa aaaacctgcc gatagttggc aggtcgtcta cgtgttaagc
     6481 tttggtgtgg tctttagctg gcaattggtt ttctaaatca gttcggacaa caagcacatc
     6541 gcaagctgcg tttctaataa catattcaga aacagaacca ataaataaac gttctacagc
     6601 attcaatcct gtggctccta acataatcaa atctacttga ttatcttctg ggatttctct
     6661 ggcaataatt tgttttggtg agccatattc tacgacagag gttacattat tcaagccagc
     6721 tttcttcgca ttgctttcat aatctgccaa tgtttgcttt gccatttcag tagcttgttc
     6781 tgcaagcatt ccatcaaagg atgaaacggt ttggaaagaa cgtgtgtcaa tgacatgtgc
     6841 taatagaagt tctccgttat ttcggatagc tacattgacc gcctttttaa aagctaattc
     6901 agcttcatca gaaccatcta cggcaaccat gatttttttg tattgttgta acataagcat
     6961 tcactcctca agtcaatcgt attgtactct tttattgtag aacactttag ggaaaaagag
     7021 aattatttac tgctttggtt cagcaatttt ttttgcaaat cggatactga aggtggcaga
     7081 aacaataatt acgatcagta aaaagaccaa ggcaataaag cgttgattga aaaaagctgt
     7141 taaaataccc attacgccta aaacagcata tgcagcaccg taaaattgta ggaatccttg
     7201 atttttatca tttttgttga tcaatacaaa aaagatatcc tgtttattaa ataaataata
     7261 gctaacgaat aataatacta aagtggcgat aatcattaaa atcctaatca tgaacaaatc
     7321 ctccaaaata agcaacaaat agaaaaagca atttgccaag agcaaattgc tttattcttt
     7381 aaaagatggt aatctccgtt aatgccgttg tttgtcctgt agtattgatc ccgcgaattc
     7441 aagcaggtgg gtcccatggt cgtagcttcg aactaccaaa tgataaggca tgttaccagc
     7501 agtgacacaa gacgggtccc aagatatcaa taaaaatgtt cggtcaacac acaaagagga
     7561 caattcgaac atcacagatt tcccacctct atagtagcat atgaatttaa aaagagcaat
     7621 gagagagctc gcagaaacag aaccgttttc ttgtctagtt ttttggagga tgctttttcc
     7681 actcttttat acggtaatac tgttgaccca gagcctgttc atatttccct gtatcttttg
     7741 gctgataata ctgagcattc ttcagtttat ctggtaaata ttgctgattg acccaagcct
     7801 gatcaaaatg atgaggatac tgataaccca cgccacggtt gagtgatttc gcacctttgt
     7861 aatggctatc tctcaaatga tcaggaacat ctccagcttt gccctctcga atatcagcaa
     7921 gtgctgcatc taatgccatg taggcagaat tggattttgg cgacaagcaa agatcgacga
     7981 cgacgtctgc tagtgggata cgtgcctcag gcaatcccaa tttttcagca gctaatacag
     8041 cgttcactgt acgagccgca gcagcaggat tacctaaacc tatatcttca taaccgataa
     8101 ccatcagacg ccggcaaata cttgccagat cacctgcctc aactaaacgg gcgagataat
     8161 gaagtgcagc atccacatca cttccacgaa tcgatttttg aaaagccgaa ataacgtcat
     8221 aatgagcatc tccatttttg tcatgggtaa gagcttttct ttggacacat tcttcaatga
     8281 tcgacagtgt aagatgaatc cttccttctt tatctgaagg agtcgatagt gtagccagtt
     8341 ccagcccatt caatgcactc cgcaaatcgc cgttcgttgc acgcgaaagg tgaagaagag
     8401 catcttcgtc taactggatt gcttgttgtc caaggccgcg ttctttatcc cttaaagcat
     8461 gttcgactgc caactggatg tcttgttctg ttaatggttt gacttcaaag atctgtgtac
     8521 ggctgcggat cgctggattg atggtaatat aaggattctc agtagtcgct ccgatcaaga
     8581 tgattcggcc actttctaaa tgaggaagca gaaaatcctg ttttgtttta tctaatcggt
     8641 gtacttcatc caataagagg atcactgtac cactcatttt tgcttcttcc gctacaactt
     8701 gtagatcctt ttttgaatct gtagcggcgt ttagcatgcg aaatgcataa ttagtagagc
     8761 cggcgatcgc acttgcgata ctcgtttttc cagtaccagg aggtccatat agaatcatgg
     8821 aagagagcat gcgagcatcg accatgcggc gtatgatttt tccttccccg actagatgct
     8881 gctgaccaac gacttcatcg atcgttcgag gtctcatgcg ataagccaat ggctgttgca
     8941 taggattttc ctccttcttg tttttacgtc ccttaattat atcatattta gcaagatgcc
     9001 tagaaagaac agccttctgt tgttttgttt tcatagctta tccaactaat attgtgtatg
     9061 ataaagtagg tgagaaaaat gacggataaa caaaagatag atgaattttt ttggaataaa
     9121 acgacgccag aagtggcacg ggatttattg gggatgtatt tggagcatga cacaccagaa
     9181 ggaagattag ctggatatat tgtagacgcg gaagcatacc taggtccaga agacgaggcc
     9241 gcccatagct atggtttaag aagaacaccg cgtgtgcggg caatgtatga aaaaccaggt
     9301 acgatctacc tttacacgat gcatactcat cggattttga acatcattac tcagccagaa
     9361 gggattcctc aaggagtaat gatacgtgca atcgaacccg ccgcaatgat tgatcaaatg
     9421 agtaaaaatc gtggcggaaa aactggtcca gatatcagca acggtccggg aaaattagtg
     9481 gaagcattag caattcctca agaactttat ggtcagtcta tagcagatag ttcgttacgc
     9541 ttagtttttg agaaaaaaaa gacaccaaaa aagatcaatg ctttgcctag aatcggtatc
     9601 ccaaacaaag gtgtatggac agaaaagccg ttgcgttttg ttgtgtcggg aaatccttat
     9661 atctctttac aaagaaaaaa ccaaatcgag aaagattggg gttggaggaa agaaaatgaa
     9721 aaagaaggat ctattaacat atttagatga aataattgaa aaacaagtga cagactatga
     9781 tgtagcttta gattgggata caaaaaatca tacgatcgag atcgttgttc gcttgtttgc
     9841 agaaaataaa gcacaatttg agattgatga tgcagaaggg atcgtttcgc aagaaccaat
     9901 tatcgaattt gaagacggcg tacttctttt taatcctcag aaatctgttt ttgatgaacg
     9961 caattatcta gcagtaatcc cttatgaagg aaaaaaaggg ttggcaaaat cagtagctga
    10021 ttctttagtg gaatatctaa atgaagtatt agctcaaggg caaagcgatt tgcttgattt
    10081 tttggatgaa gacgatgacg aagctgtatt cgagcttcac tgggataatc aacaactagc
    10141 agaattagta gaagtaaaac agcaaaatga agcagatact tatttaccgt acccaagtta
    10201 ttaaggagtg acagatatga aatggaatga agtaaaagta gaaactgaaa gtgaagcagt
    10261 cgaagcagtt gctaatattt tgatggaggc aggggcgagt ggtgtcgcga ttgaagattc
    10321 tttagatgtt gaacattttc agtcggatca atatggtgag attttagata aaaatgattt
    10381 tacatcgctg aaagaaggcg cactagtcat ggcttatttt ccggaaacga ctttcttacc
    10441 ggaaatcttg ccttttatca aagatcgtat cacacaatta cctgagtttg gtctagctat
    10501 tggaaaaaat aaagtaacag ttagtgaagt agaggaaagc aactgggcaa cagcctggaa
    10561 gaaatactat catccggtgc gtattacgcg aatgctgacg gttgcgccaa gctgggaaaa
    10621 ctaccagaca gaagatcctc tagaaaaaat tatcacacta gatcctggta tggcatttgg
    10681 cacaggcact catcccacga cttccttgac attgacagca ctagaaacag tcttgcgtgg
    10741 tggtgaaaca ttgctagatg tgggaactgg gtcgggcgtg ctaagtattg cagcaaaata
    10801 tcttggtgca aaagatgtct atgcctttga tttagacgaa gtagccgttc gttcggcgaa
    10861 agaaaatatg gatatgaatg aaatagccaa agatgtccat gtatcagcta acgatctttt
    10921 gacaggaatc gagattgaaa gtgatgtaat cgttgcgaac attttagcag atatcatctt
    10981 attgatgatc ccagatgctt ggcgtttact gaagcaaaca ggcacgctga tcgtttcagg
    11041 aatcattgaa gagaagaagc aaatggttct ggatgccatg ttagaacaag ggtttatcgt
    11101 ggatcaaatt ttccagcaaa aagactggta tgcaattatt ttgaaaaaac cagaggtgga
    11161 atagatgcag cggtattttt taaaagaatc atatgaatca aaagactatt ttgttttaga
    11221 aggggaaaac tatcaccaca ttgtacgggt tatgaggatg gaacccgagg atgaagtttt
    11281 tttggcattc aatgatcaaa tagccattgt tgcagaaatc acggagatta ctgaagaggc
    11341 tgtttatctg aaagaaatta aaaaagaaga aaatcaaaag gaactgccaa gttcgattac
    11401 catagcgagt ggctatccta aaggggaaaa gctggattgg atcgttcaga aatccactga
    11461 attaggagcg caagcttttc ttggtttccc ggcatcagcc tctgtcgtta aatgggatgc
    11521 aaaaaaacgt atgaaacgac aacaacggct tgaaaagatt gctcaagagg cagcggaaca
    11581 atcccatcgg cagatcgtgc catcagtaga gttattggaa acgaaacaag atttatttga
    11641 gcggctagat gattatgacg tggtcttggt tgcttatgag gaatcagcaa agcaaggcga
    11701 aaaatcaaat gcagccaaaa ttcttagtga aacaaaacca gatagtcgaa tcttagctgt
    11761 ctttggacca gaaggtggat tatcccgcga tgaagtcgat tattttgtag aacacaaagg
    11821 gatactttgt ggattaggac caagaatcct tcgtacagaa acagcacctc tttatttatt
    11881 aagtgctgct agctatcaat gggaattaat gaatccttct ttcagctaac gttcttcatt
    11941 aataaaagag tatcgacaga aagaattgaa aaaatagtca acaatttgta taatagaaac
    12001 aattatttat acaatggata cgaaaaatcg tttgaaaaag cttttaaaaa gcgaacattt
    12061 ccgtaaaaaa acgtataact gcacggattt tgggacaatt atcaaacaga tatttgtccc
    12121 gattcccgtt ttaccgaaaa aagaaagggg gatcgattcc tgtgacaaaa gaagaaattt
    12181 taacgggacc cggtgtcata aagctcgtta gtcaatacat gggtcccgaa catgtagcat
    12241 ttgttgagaa agcatgcgaa tacgcgactg ctgcacatga cggacagttt agaaaatcag
    12301 gcgaacccta cattattcat cctatccaag tcgcaggtat attagcagat ttaaaaatgg
    12361 atccccatac agtggctaca ggcttcttac atgatgttgt tgaagataca gaaatcactt
    12421 tagaagatct gagagaagaa tttggcgatg acgttgctat gttagtggac ggcgtaacca
    12481 aattagggaa aataaaatat aaatcccacg aagagcagct agcagaaaac caccgaaaga
    12541 tgctgctagc aatggctcaa gatttacgag ttatcatggt caaattagct gatagacaac
    12601 ataacatgcg tacgttgaag cacctgcgag aagataagca aagaaggatc gctcaggaaa
    12661 ctttagaaat ctatgcgcct cttgcacatc gtctagggat cagccggatc aaatgggaat
    12721 tagaagatac agcacttcgt tatctaaatc caaaacagta ttaccgtatc gtccacttga
    12781 tgcagacgaa gagagaagaa cgtgaaaaat acgtaagtgg tactgttgaa gatattcgaa
    12841 tagcgacaga agagctgggg atttttgcag aaatctatgg acggccaaaa cacatttatt
    12901 caatctatcg taaaatgaaa gatcagaaaa agcagttcaa cgaaatttat gacttgctag
    12961 ctattcgagt gatcgtagat tcaatcaagg attgttatgc tgtattagga gcaatccaca
    13021 caaaatggaa accaatgccc ggcagattta aggattatat tgccatgcca aaagctaata
    13081 tgtaccaatc tttgcatact accgtgatcg gtccggcagg aaatccggta gaaattcaaa
    13141 tccgaacaca ggaaatgcat gaaatcgctg aattcggggt tgctgcacac tgggcctata
    13201 aagaaggaaa aaatgaaaaa gtagaaccag atggtatgac gaaacaatta agctggttcc
    13261 atgagatact cgaacttcaa gacgaaagct atgatgcttc tgaatttatg gaaggcgtaa
    13321 aaggagatat ctttagtgat aaagtctacg tcttcacacc aaaaggagac gttactgagt
    13381 taccaaaagg atccggacca ttggactttg catacagtat ccatacagat atcggtaaca
    13441 aaaccactgg tgcaaaagta aatggcaaaa tggtgcagct tgattacaaa ttgaaaaacg
    13501 gagatatcat tgagatcatg acttctccaa attcatttgg cccaagtcgc gactggttga
    13561 aattagttgc tactagcaaa gcaagaaata agatcaaacg tttcttcaaa gcccaagatc
    13621 gagaagaaaa tgtgatcaaa ggccacgaat ccgtggtcaa atgtattaca gatctaggat
    13681 ttacgcctaa agatattttg acgaagaaca aactgcaaga agcactcgat cgttttaatt
    13741 atcaaacaga agatgatctc tatgcagctg tagggtatgg agaagttagc cccttgacga
    13801 tggccaatcg tctgactgaa aaagaacgta aagaacaaaa aatcgagcag caaaagcaag
    13861 aagcagaaga aatcatgaat cagccgaaaa aagaacctga caaaatgaaa gtacgtcatg
    13921 aaggtggcgt tgtcattcaa ggggtagaaa acttactaat tcgtatcagt cgctgctgta
    13981 atccgattcc tggtgatgat atcgttggtt atatcactaa aggcagaggg atatccattc
    14041 atcgtcgaga ttgtccgaat gttcagcctg acaaaccaaa tgtagcagaa cgtttgattg
    14101 aagtcgaatg ggaagataca tcgaatacac gaaaagagta tgatgcagat ttggaaattt
    14161 acggctataa tcgttcaggc ttattgaatg atgtacttca aacagtcaat gcgctaacga
    14221 aaaatctcaa cagcgttgaa gcacggacga ataaagataa aatggcgacg atccatttga
    14281 cggttggtat ccagaattta tcccacctaa agagtatcgt ggataaaatc aaagcagtac
    14341 ctgatgtcta cagtgtacgc cggacgaatg gataggagga cagatatgag agcagttgtt
    14401 caacgggtaa gtaaagctgc tgtgtcagtc gatcagcaag aaattggaaa aattggctac
    14461 ggcctacttg ttttattagg cattcatgaa accgatacgc aaaaagatgt ggactatctg
    14521 gtaaaaaaaa ttgcacaaat gcgtattttt gaagatgaac aaggaaaaat gaatattagt
    14581 gtagaagatg tgggaggaca aattttaagt atctctcaat tcacactgtt tgcggatacg
    14641 aaaaaaggaa atcgaccaag ctttgtggaa gccgcacgac cagaaacagc tattccttta
    14701 tacgaatcat tcaatgaagg aatcagaagt cgcggcatca cagtcgctac cggacaattc
    14761 ggagcagata tgtcggtttc tctcgtcaat gatggacctg taacgatcat tattgattct
    14821 aaaaacaaat aaagtaatct ggttttcact agtaaaaact atgaaacacg tcgcttaagt
    14881 tagactgata aactctaact taagtggctt ttttgtctgc aaaaagcgct tcttttgtca
    14941 gcagtcggaa aaaaagttaa gcttaaatta tacaagttat tttttatagg aggttgctga
    15001 agatgaaaga agtaccagta gtagatatca aaatcacaga tttaaaaaaa ttgtatcaaa
    15061 aaggatacaa catgttggag gagttacgcc atgaagcgga cgctcccgtc gtcaaagcaa
    15121 agattttcaa taaagaagct atcacgatct acggatcatc agcagcaaaa gtattttatg
    15181 atccaaggaa tttcaaacga aaaggtgcta tgccaaaatt agtattgaag acactttttg
    15241 gtcaaggtgg agtacagact ttggatggag ctgctcatca ccatcggaaa aatattttta
    15301 tggatctaat gacaccggaa agaatggaag attatcatcg tatcttagat aagaatctga
    15361 cacaagcact tgaagcacag catggtcaat ttgaactatt tgatttaagt aagatggttt
    15421 tctttacatc tatttgcgag tgggcaggta tcaatttatc agcgatttct aaagatgagg
    15481 tcgaaaaatt agcagaatat caaatctcta tgatcagcgg aacgtttact tctccgattg
    15541 atcacataaa aggtgtcgag aatcgcaaaa aatcagaaaa atgggcgcaa ggcttgattg
    15601 aagaagctcg acaaaatcct gtggctggaa aagaaaatgt ggcattatat gcttttgcca
    15661 atgccacaga tttagatggt cagcttttac ctctggaagt ggcagctgtt gaattattga
    15721 acatcatccg tccaacggtt gctctaactg tgtgggcggc attgatggga catgcacttt
    15781 tcagtagacc agatttgtat cagcaattaa aaaatgactt ttcgacgtta caagatccgt
    15841 ttattcaaga aatgagacgc tattatcctt tcttcccaat gctacctgcg atttcgctga
    15901 aagaagtaga agtagatggt tatcgtatac cagaaggcag ttgggttatt ttagatttat
    15961 atggaacaga ccatgatgaa cgaacagttg aagcgccaga ttcctttatg atcaaacggt
    16021 atgtagggaa agcaaaagat atttcctata aagaagaata tgagatgatt gcacaaggcg
    16081 ggggaaattt tcgacaaatg catcgctgtg caggagaatg gatcactctt catagtctac
    16141 gtgtgttttc cgatcagcta gtaaataagt ttgagttctc tgtacctgaa caagattgga
    16201 cgattccatt caatcagttt ccgacttatc cgaatagccg agcgctactt tacaagaatt
    16261 agattagcat aaacttatcg aaaaaaagga agatcaaaca gtgctgatct tccttttttc
    16321 atattactat aaattgataa ggtcaattga tcaaaatcca cgaaatgctt catcaatggt
    16381 ttgataatct tcattggata aatgcaaatc taaagcttgg acattgtcag atacttgctc
    16441 tggacgctta gctccaggta taactacaga cactcgtgga ttcttcatgt accaagctaa
    16501 aacaagttgc gcaggagtgg cttggtatct tttagcaatg gggcgaagta catcgacagc
    16561 ggttaaaatt tctctgaaac gttctccttg gaaatctgga tttctttttc gaggatctcc
    16621 ttcaccaaat tgtttttcct caccaagttc atattttcct gtcagcaaac cggaagcaag
    16681 aggaaaatag ggaacaaaac tgattttgtt tttttcaaga taagggaata attctttttc
    16741 agcctgccga tggatcaaac tgtatttatc ttctactaca tcgacatatc catctgcatt
    16801 cgcttctttc aattgttcta acgtgaagtt tgacacaccg actgctcgga tttttcctgc
    16861 ttcttttaat tcgtgcaatg tagcaactga ttcatttttt ggtgtgcttt catccggaaa
    16921 gtggatataa aaaatgtcga tgtaatcagt ttgcagacgt tttagtgcat cttccaccga
    16981 ttgtttgaga aactcaggag aattatcaaa tgttcgccct ttatttggta catgagcagc
    17041 ttttgtagcg atgacgacac gtgagcgatc atattctttt tccttcagta cctcgccaat
    17101 cagttcttcg gaacggccgt ttccgtaagc ataagcggta tcgatcaaat tgatcccgct
    17161 attcaaagct gtacgaacta cttgttttcc tgtctcatct tccaaaccgt caaaaagatt
    17221 atgtcctcca acggcatttg ctcctaatcc aagctgttct gcgtagactt gtgaatgtcc
    17281 gattcttatt tctgccatag ctgattcctc ctttttgtct agtctaaaac aaaataccaa
    17341 aaagctgaac taaaacgttt ttgtgagggg aaatagtcag atatctcttg actttgagac
    17401 tggtttttag taggttataa gtaatatcga atgtcaatga aagagcgagt aagtttttga
    17461 aactttacaa gagaggatca tcgaggctga aaatgatcta aagtttcatt agcctaaaga
    17521 cactctggag atggacgagt gaaaagccgt cccgcgtacg agcgttattc gtaagaggaa
    17581 agagaaatct ttcaaaatta ggtggtaccg tgtatgaatt tcattcaccc ttgttcatga
    17641 tgaacaaggg tgattttttt atttacatag ctaagaaagg gagagaaaac tatgagtttc
    17701 caaagaccaa aaggaacaaa tgatattttg ccaggcgaat cagaaaaatg gcagtttgtc
    17761 gaagaaacag cacgtttatt attcagagat tatcagtata atgaaatgcg tacaccgatt
    17821 tttgaacact atgaagtgat atcccgaagt gtaggggata caacggatat cgtttcaaaa
    17881 gagatgtatg atttttatga caaaggggaa cgccatgtga cgcttcgacc agaaggtaca
    17941 gcaccaatcg ttcgttcttt tgttgagcat aaattgttcg gcccagagta tgcgaaaccg
    18001 ttcaaaactt tttatatggg accaatgttt cgctacgaac gccctcaaaa aggacggtta
    18061 cgtcaattcc atcaaatcgg agtagaagca tttggatcag aaaaccctgc aactgatgtg
    18121 gagagcatgg tgatggcact tgatttcttc aaacagttgg ggatcacgca aatccggcta
    18181 gtcatcaatt cattaggtga taaagaaacg cgtcaagcct atcgtcaagc attgatcgac
    18241 tatcttttac cttttgaatc agagctgagt gaagactcta aacgccgcct tcatgaaaat
    18301 ccattacgcg tattggacag taaagacaaa cgagatcaaa aatttgtggc agaagctcca
    18361 tctatcttag attatctaag cgagcctgct caaaaacatt ttgataccgt tgtttccatg
    18421 ctggatgcat tggctgttcc ttatgaaatc gacagtaata tggttcgcgg actagattat
    18481 tacacacata cgatttttga aatcatgagt gacgcaccaa aaatgggggc acaagcaacg
    18541 atttgcgcag gcggacgtta tgacaatcta gtggaagaac taggtggtcc ttcaacacct
    18601 ggcttcggat ttgcaatggg tatcgagcga ttactcttga ctatggaggc agaggaagta
    18661 gttatccctg catttaatga gttggatgct tacgtggtcg cactcggaga cgaaacaaat
    18721 atcgaagcat tgaaagtggt ccaagcgatt cgaaactttg gtttttcagc tgaccgcgac
    18781 ttcatgaacc gtaaagcaaa agcgcaattt aagacagcag ataaagcgaa tgccaaatta
    18841 gtattggtga tcggtgaaga tgagcttgct agtcaaacga ttaatgtcaa agcaatggca
    18901 agccgaaaag aaaaaagttt tccattagaa gagatatatg aacattttga tgaagtctat
    18961 gatgaaatga ctttattcga gtaggaggaa aaaaataaat ggcacaaaga acgatctatt
    19021 gcggattagt ttccgaacaa caattagaac aaacagttac attaaaagga tgggtacaaa
    19081 aaagacgaga ccttggggga gtaatcttta tcgacctacg tgatcgtgaa ggaatcgttc
    19141 aaatcgtctt taatcctgaa atcaacaaag aagcatggga aatcgcggat aaatgtcgca
    19201 gtgaatacgt gatcgaagtg actggggaag ttagaagacg cggagaaaat gcagtaaatc
    19261 ctaaaatggc cactggtgaa ttcgaagtga ttgcaagtga aatcgtgatt ttgaatcaag
    19321 caaaaacccc gccatttcaa attgaagatg atcaagcaat cagtgacgaa atacgtttga
    19381 aatatcgtta tttggatctg cgtcgtccaa aaatgactaa aaacatcaaa ttaagaaatg
    19441 cgacaacaaa agcgattcgt cattatttag atgataatga ctttctcgat atcgaaacac
    19501 cttacctagg gaaatctaca ccagaaggcg caagagacta cctagttcct tcacgtgtcc
    19561 atgctggaca tttctatgcg ttgccgcaat ctcctcaatt gtttaaacaa ttgctgatga
    19621 atgctggatt cgaccgttat tatcaaatcg ttcgttgttt ccgcgatgaa gatttacgtg
    19681 gagaccgtca gcctgaattt acgcaagtag atattgaaac aacgttctta acagcagaag
    19741 aaatccaaac ttacacagag ggattgattg cgaaggtaat gaaagaagtt cgcggtatcg
    19801 aagtcacatt gccatttcct cgtatgacct atgatgaagc aatggctcgc tacggcagcg
    19861 acaaaccgga tacacgtttt gatatggaat tgatcgatct aagcgataca gtcaaagaag
    19921 tagaattcaa agtcttccaa atggctcttg aaaacggcgg tgtcgtgaaa gcattgaatg
    19981 ctaaaggcgc tgcagatcgc tactcacgaa aagacatgga tcagttaggt caatatgtag
    20041 gacaattcgg agctaagggg cttgcatggc tgaaagtaga agaagacgga ttgaaaggtc
    20101 caattgctaa attcatggga gaagcaacag aagcaatcat taaagcaaca gatgcaaaac
    20161 caggtgactt gctgatgttt ggcgcagata aaagtgaaat cgtcgctgct gcattaggtg
    20221 ctatccggac acgtttagga aaagaattag gactaattga tgaatcgaaa ttcaatttct
    20281 tatgggtaac tgattggcca caatttgaat tttcagaaga agaaggtcga tatgtctctg
    20341 ctcaccatcc attcacgatg ccaaaagaag aagatatccc gttattggct gaagaccctg
    20401 caaaagtcta tgcagaagct tatgatatcg tcttgaacgg ttatgaatta ggcggaggtt
    20461 ctatccgtat tcatactcgt gagcttcaag aaaaagtact agaaactcta ggctttacaa
    20521 aagaacaaat ggaagaacaa tttggtttct tgttaacagc tttagattac ggattccctc
    20581 cacatggcgg tattgcatta ggattggacc gttttgcaat gttattagcc ggagaagata
    20641 atattcgtga agtcattgcc tttcctaaaa acggaaaagc agcagatgtg atgagtgatg
    20701 caccaagtat ggtttcgcct ttacaattgt tcgaattgaa tattgatgta acgaatatag
    20761 aagaatagcc gataaaaagg taaaaaggat ttgtcgaata cgcttctttt gacaaccttg
    20821 atttaattga aaaacacttt ataatgaagc cataacatat aaagcggggc ttttccatga
    20881 aaaaaacatt cgaaaattgg gcttatcatg attacacgac caagctttcg gtatctatcg
    20941 tttatgcgtt gatcgcatca gtcgcattga atttctttta tcagccagga catatttatt
    21001 caagtggtat tactggtcta gctcagattt tgacgacttt gtcaaaatca ctgataggat
    21061 tcgaagtacc ggtatcaaca actttgtatt tactgaatat acctttgttt gtattggcgt
    21121 ggttaaaaat cgggaagaag tttacaattt tcacttttat cacagtcact ttgacctccc
    21181 tttgtatcca tattttaccg gaaacagtcc tatctaccga tccaatcatc tgtgcgttgt
    21241 ttgggggggc tgtgatggga ctaggtattg ggctagcgtt gagaaacggc ctatcatcgg
    21301 gcggtctaga tatcgtgagt atcacgattc gtaaaaaaac aggaaagtcg attggttcta
    21361 tttcgatcta cttcaatgca ttgatcgttt ttgttgccgg aattctattt ggctggcaat
    21421 atatgtttta cagtacgttg tctattttcg tcagtggaaa agtaactgat gtcgtttata
    21481 ctaaacaaaa gaagatgcag gtaatgatcg taacgaaaca tccagaaaaa gtagtaaatg
    21541 aaatccaaaa ctgtatgcgt agagggatca cgatcatcaa tgaagcagaa ggtgcttata
    21601 agcatgataa gcagacagta ttgatgacga tcgttacacg atacgaactg cctctactta
    21661 gagaagcaat gaaagcttct gatccacaag cttttgtaag tattgcagaa aacgtccaga
    21721 tacttggtcg tttctatgag gatggcattt agatcgagtt tgacaaaaat tcatgaaata
    21781 ctgctgtaga cagacgttcg tttgtctgca gtttttttct accatagtag aatcagcaaa
    21841 gaaaaactgc ttttttattt gaaattttct taaagttgaa aaaaagcatg tttatttttt
    21901 taaatttatg ggatgataga aaaaaatagt agagaaagga cacgattcta cttgctattt
    21961 tcacttaata cccgtatatt acgaatgaaa aaagtttatg aaaagttgga atattgaatg
    22021 gaaaaacaaa aaaggttaaa aaacagccgc tacatcactg ggtttgatgg gctccgttca
    22081 ttagctgtgg tgggtgttat cttctatcac cttctgccaa ctagtatgaa aggcggctat
    22141 ttgggcgttc cgattttttt cgtcgtatct ggttatttga tcacggatct gctgcggcag
    22201 gaatgggaac aaaatggaaa aatcaatatt tggcaatttt atgtcagaag aatgaagaga
    22261 ctctatccgg cacttgtttt tatgcttgtg acagcttcgg cttatatcac tttgtttcag
    22321 cgagggctgt taaacaatct tcgaggagtt gtggtcagca gcttactgta tgtcaacaac
    22381 tggtggcaaa tcaataacgg tttatcttat ttcgatcgat ttgctaatga gtcaccgttt
    22441 acgcatatct ggtctttagc agtagaaggt caaaactatt tggtttggcc gatcttgttt
    22501 gtattgttga tggtatttgt tcgtaaaaga aaatggatct tttatactgt gttaggtaca
    22561 tccttcctct ccgccatctt gatgatgatc ttgtatacac caggaggcga tccaacccgt
    22621 gtttattacg gtaccgacac tcgtctgttt tctatatggt tgggaagtgc gcttgcgttt
    22681 gtttggccaa gtacgcgttt aaagaaaaac atcccgaaac aagcgaaaaa tattctgaat
    22741 atcgctggag gaatctcgct tttggcactg attctttgct tcttcttttt agatgatcat
    22801 tacaatttca tctattacgg aggaatgctc ctagtgagtc tgttatgcgt agtgctagtt
    22861 gcagtgacag cacaccccgg agcaagtttg aatcgatggc tgacaaatcc agtatttact
    22921 tggattggca aacgaagcta tggcatttat ctgtatcagt ttccagttat gattttttat
    22981 gaagcaaagg tgactgatat tgccgatcat gttttgttgc atactttagt ggaaatcaca
    23041 ttgattctaa tcatcagtga actttcttat cgatatgtgg agcgtccgct agcacgtttt
    23101 gattatggtc gtacattcca agtgatgaaa ggatggttca gcaaaccgat catcagtaag
    23161 agcaaaccat gggttgttcc aggcacatta cttgtagcaa ttgcactcgt tggtttcgtt
    23221 acggcaccga aaaacgctgt aacagccgat caaaaacaac tgcaggcaaa aatcgctgaa
    23281 agtaaaaaat tagcagaaga aaccagaaac aattcagaaa acaccactag ttcggttgac
    23341 caagcgatcc ttgataagta cgagttgtcc aaggcacaag ggaataaggc acaaaaatta
    23401 gaactaacgg catttggtga ttcagttatg ttggatgctg caacggatct acaagaactt
    23461 tatccaaaag tagtggtaga tggagatgtc ggacgacaat tatatgccag tgtaccgtac
    23521 attgaaaaat taaaggaaca agatttgttg aaagacactg ttctgattgg actaggaacg
    23581 aatggctcat tctcggaagc tcagtttgat agtgtgatgg atgcaatcgg tgatcgaaaa
    23641 gtatactggg tcaatgtccg agtaccgacg aagcgctggc aaaatgacgt gaatgcgatg
    23701 cttgaacaga tgacgaaaaa atacgacaat atgacgctga ttgattggta cagctacagt
    23761 aatgaccata gtgactggtt ttatgatgat caagtccatc caaatcctga agggatgaca
    23821 cattacattc acttagtcag cgaagccttg ctaggaggaa gttcaagtac gaacagcagt
    23881 gagacaaacg tgacagaatg atagctacaa aaaacttcag gcaaaaacat ttgtttttgc
    23941 ctgaagtttt tttatattta ccgtgttttt aacattcata tagattcagg aagcaaaacg
    24001 gttgagtgtg tctgtaaata acgttctttt tgttttcggg tgagtttttt gaatgcagct
    24061 tctgcttttg ttgcttcgct tcttgaagaa aatacttctg catggatcat cttaacaggt
    24121 cttctttttt gaagacgagt atatttagca ccagtaccat tgttatgttc ctctaatcgt
    24181 ctagaaagat cggttgtata tccaccataa aatgtctgat ctttacagaa taatacgtaa
    24241 aaataatgtt catttgccat agagcatctc acgaatctct gcagtgtatt ctccatccat
    24301 attgtaagtg atcaatggtg gagcgacttt gaatccgtct aatttgcctt ctttgattcc
    24361 ttcaactaac aaaatatttg cctcttttcc aggttttggg taaacaaatt ggatcctttt
    24421 aggtgcaatt cgatagtctt ccatgacctg aatgatttct aagaaccgat caggacgatg
    24481 gaccatagta aagcgtccat ttgtttttag tagcttactt gaaatatcga tcacttcttc
    24541 taacgtcgta tgaatctcat gacgggcgat tgccaggtgt ttattagggt tcgttttatt
    24601 tgttggcagc cctttgaaat aaggtggatt acagacgact aagtcacaag aattatgtcg
    24661 aacgacagat aagctgtctt tcagatcgat ggtgtggaca gtcatttgtt catttaaatg
    24721 gttaagcgcg atactacgtc ttgccatgtc tgctagacga ggttgaagtt cgatagcgtc
    24781 gatagccgcc tgggttcgtt tacttaaaaa aagtccaact gcgccatttc ctgcacaaag
    24841 gtcaacaatc ttcccttttt taggtacacg ggaaaagtga gcaagcagca cagcatctaa
    24901 agaaaaagaa aacactttgg aactttggat aatcttgata tcttctgcgt ataattggtc
    24961 gattcgttcg tcttcgtgaa gcatggattc cctccaaaat gaaaaaaata gtcgtctgta
    25021 ttatactatt tttattaaaa agaaccaagg tggcttgtca aacctctatt ttttatgcat
    25081 actatgacca taagaaggga aaggaagaga ctatgtttta tcgatttatg cgcggggtag
    25141 ttcgagtact attagcctta gttaatggaa atgcccgcta tgagaataaa gaagttttac
    25201 cgcaagatga gaattacata ctagttgctc cccatcgtac ttggtgggac ccattatatc
    25261 tggcggtagc tgcagcaccc aaggaattca gctttatggc aaaagaagaa ctgttcaaaa
    25321 atccagttct gcgatttata ttaactcgat ccaatgcttt tccagttaaa agagaaaatc
    25381 caggaccaag cgtcattaaa acacctgtaa agattttgaa atcgacaaat ttgagtttga
    25441 tcatgtttcc cagtgggaca agacatgcta cagaacttaa aggcggaatg gcattgattt
    25501 ccagaatgtc taaagcgaaa atcgtgccag ctgtatatca aggtccgttg acacttgggg
    25561 atctgttcaa gcgtcagaga gtgacagtgc gtttcggcga accaatcgac ttatcagata
    25621 tcaagaaaac ggataaagaa ggtatggcgg agatcgaacg acgaacacaa gcagcttttg
    25681 acaaattgga caaagaagtg aaccctgatt tcaaatatga agtcaaaaaa aaataaaatg
    25741 ataagtttat gcgtgggcgg tcttttgaat ctactgtcct gttcagttca gactttttca
    25801 atgaaaacga gggagagacg tcaagtctgc cctctttttg cgttccaata tgtaaaatag
    25861 aagacaggtg tcatgtaatg gatcaaagtg ttataataat tagaaggaca ttttttgagg
    25921 tgattgaatg cgttttttac atacagccga ttggcatatc ggaaaaaaac tacaaggtta
    25981 tgacttgatt gaagaccagc gtcatgtact atcttctata ctagatatcg cgaaagaaga
    26041 aaaagtagag gcaatcgtga ttgccggaga cttatatgat aggagtgtcc cagctgttga
    26101 tgccgtagaa ttatacaatg aattgatggt cgattggaat ttaaaggaaa agatcccgat
    26161 ttttgctata tcaggaaatc acgatagcag tacgagactt tcagcgggta cgccatggtt
    26221 cagttattcg aactattttt tgcgaacaca gctagcagaa gcatttgaac cagtcgagtt
    26281 taaaaacgta caatttttct ttcttcctta ctttgaacca attgcagcga gattatattt
    26341 tgaagatgac aatatccgaa cgatcaagca agcggtagcc aaggtgatag ataaaatgca
    26401 ggagctattt gattcagaaa aaaaacaagt attgattagc catttttttg taatgggaag
    26461 tgtaaaatct gattcggaaa cgaaaatcga agtaggcggg ttggattcga tccctggtga
    26521 actacttaca ccgtttgatt atgtagcact aggacatttg cataatcaag ctgctttgag
    26581 acaggataat gctcgctata gcggttcccc attgaaattc tctttatctg agatcaacca
    26641 aaaaaaaggc gtatggatcg ttgacctgac aacaggcctg gagttgaatt ttagggagat
    26701 cgaacccctg catgatattg aagaaataac agcgagtttc aaagaattgc tggatcctgc
    26761 cttttatcag tccgtcaatc gggagaatta catctatatc tatctcactg atagaacgat
    26821 catcccgaat atgatgaacc agcttcggca aatatatcct cgtattttgg ggattgaacg
    26881 ggcaaacggt cgagcagaac aaacaaagcg caaacgaaaa gtactagaaa tgaaagatcc
    26941 tcaaaaatta gcagaagcat tttttagtga aatgacggaa gagcagctaa ctgcaaacca
    27001 agaaaaatgg ctcggagaaa cgttgcaaga aatcatggaa atggaatgat caagaatgaa
    27061 accgaaaaaa ttacaactta aaaattttgg tccttttatc aatgagaccg tagatttttc
    27121 acgtttgaca gaagcaccgc tttttttgat cagcgggaag acaggagctg gcaaaacgac
    27181 gatttttgat gggatcacct tcgcactttt tggtgaaact tccggaagac ttcgctctgg
    27241 aaaagagatg cgttctttgt ttgctacacc agacgaagaa acaagcgtga ctttttcttt
    27301 tgaacatcag cagatgagct atgaaatcga acgaaaacca gaacaagttt tagcaaaacg
    27361 aaaaggaaac ggaacaaaaa aacaggcagc caaagtaacg ttgaccattt ttgatgggaa
    27421 aggaaaagaa ataaaacaaa tcacaaaacg tacagaagca gatcagttga tcagagaact
    27481 gatgaatctc gatgcgaaac agttttctca aatcgtttta ctaccccaag gagaattccg
    27541 taattttttg gtttcatcaa gcagtgataa agagactgtt ttacgtcatt tattcggtac
    27601 tcaatttttc caacagttca atgagcgatt aaaagaaaaa gcaaaacaac agcaacaaaa
    27661 attggatcat ttagagcaag agctgcatct tctgcaaaaa aggtttgttt tagtcaaaga
    27721 agaggaacca gcagcagggt ttgaagccgt tttagcccaa tggacaaacc atcaggcaat
    27781 actcgaaaaa caaatcacgg aagaaaaaga agctctaacg gaacttcagg aaaaacaaaa
    27841 gcaactagaa aattcctact atcgttttga aagcttgaaa aaaagttatg aagaaaaaac
    27901 gatattaata aacaagcaaa aggaattagc tgctcaaaaa gacgaaatag aggaaaagaa
    27961 acgatggatt gggtattatg aatttacgca acgattagtc gaaccaatcc agcgcctaca
    28021 agaaataaaa gttgaaaaaa tagaactgtc acaaaaaagt agtgagcaaa caaagaaact
    28081 agcgatgact acgcagaatt atgaagagtg gcaaaaaaat gaagcaaacc gcgtacgccg
    28141 acaaacaaaa atcaaagaag ctgctcaaaa actgcaaaac aaacagatga tggtgccaat
    28201 tgtcgaatcc ttacgtagta aaaaacaaga cgcagaacaa ttaacaattt cattggataa
    28261 agaagaattg aatgccgaga agctgtatca aaatctcgct atctatcagg aaagaactcg
    28321 aattcttcaa gaaaatttac tggatcagga aaaactgcaa gaagatcgtc ttgcttttgg
    28381 acagttaaaa tatctagatg aaaagcagca agaaatagaa aaacaaaaag caaaagacac
    28441 tgggtcgctc gcacagataa gagaagaact caaaaaagca actgaagaaa taacaaaaaa
    28501 tagtcagcta ttgaaagaac agacagaaca agcagaacag ctgaaaagcc aatgggcaaa
    28561 gcagcaaatt gctcgtcttc agcttttttt gcgtccaggg gagccatgtc ttgtttgtgg
    28621 atcaacggaa caccccatac aacaattaaa acacgaacag ccaaataagg aagatatctt
    28681 aaaagtagaa caacaactgg cagaagctga gtctgctgta gaaagaactc agcaaatcat
    28741 tgccaaaagt gaagcacatg aaaataatct tcaacaacaa ttatcagatt tagaacaaaa
    28801 aatcatggca acgaatatac aattggctga acaagaagaa cacttgcaac aggctttgaa
    28861 aaaggcattt cctgagataa cagaacaaaa aacaaaaaca actcttgtag taatcgaaca
    28921 atctttagat agacgaaaaa gcaagatggc caatgcaaga atagagctgg aagaaataag
    28981 acaacaagca gaaaagctcg aacaacaatt agctgtctac aaacagcagc agacagaaaa
    29041 aaataatcag ctccagcaac tgcaaggaga aatcaaaagt ttagaacaac aattgcaaga
    29101 tacggatagt aactgtttag aagaggagat acgacgactt gaaaaacagt tagaagaaga
    29161 ctcgaatcaa ctagctgttg agcagaaagc aggagtgcaa ttgttgcaag atcttcatac
    29221 gcttcggaca cagcttgatc ttcaacatga acaaatagaa aaaatagatg aaaaaataga
    29281 aaaggaacaa gaaagaatag ctaaaaagtt agaaagccag tcgtattttt caacaattga
    29341 agaaatctgt gcatttgcac aacagcaagc acaatataaa gaaaaccaac gtacagttac
    29401 ttcttatcat gaagagcaat tagtgacagc tgaccggctg cgtcaattag aaaatatcca
    29461 gctaactgag acattcccag atacagaaga gatcagccaa cagttagaag aaataagacg
    29521 tatgatcgaa ataaagcaag aaaagatata cggtttacaa gaacaaaaaa ttagtaatca
    29581 aaaaatctac gatgaatgtc tttcgattta tcacacaagc cagacgaaat tagatgagct
    29641 tacgcaatta cagcagcttt cgcaaacatt caacggagaa aatcctaaga agactagttt
    29701 ggaacggtat gtgcttcagg tttatcttca agaagtgtta caagtagcaa atgatcatct
    29761 ccaacgttta acgaaaaatc gttatcaatt tgagctagcg gataccattg gcagttatcg
    29821 agggaaaacc ggattagaaa tcaatatcta tgatgatcag gcaggtatgt ctcgaagtgc
    29881 gcataccctg tctggcggtg agagttttat tgcagcactt tctttagcat tagctcttgc
    29941 cgatgtgatc cagacacaag ctggtggtgt agcaatcgat gcattgttta ttgatgaagg
    30001 atttgggtca ttagatgaag aagctttgga aatggcaatg gaagctttag aaacgatcga
    30061 aagtgaagga agaatgatcg gcatcattag tcatgtaaga gaattgaaag aacgagtatt
    30121 acaacaaata agaattgata cagaaggtag cggacaaagc aaaatacgct atcaacttgg
    30181 ataaaaaggg agattggtaa ctatgaaatg gagtattcca gaaaaagtca ttgcacgtgg
    30241 acgtcaatac ttgcaagaag gccgagtgtt atcggttgtc cctgaccaag aagaaaacgt
    30301 gtggcatgca gaagtcttgg gcagtgaatt gtatatggtt gatttggatg gtacagcaaa
    30361 agagatagat tactgccaat gtccctattg ggaggaacat aaatattgta agcatacagt
    30421 tgctgtggaa ctttatttac ggtcgaaaaa tctatctcgt atcatgaaaa aagaccagcc
    30481 aatgctagaa ccggtaaaag caacaagtga gggagaaatc attgcaaaag gtttttctcg
    30541 tctaaaaaca caaaacaatc ctcaagcggt gcaacctttg attattgaat accaagtaga
    30601 aacgattgag actaatcagt atcacccaga gctttctatt ttggcacttt acttgcgtat
    30661 cggttcattg gcatcaccga aaaaaacata tatcgtaaaa aacatctatg attttttaca
    30721 agcttacaca gaaaaagaaa cttacactgt taataaacag taccagtttc gtctagataa
    30781 acgggcgttt caaaaagaag atcaaaaaat actcacgcat cttgcaggaa gtgctcagac
    30841 gcatcaatta ttaggtacga acggcttaca agtcaaagga aaactagata aaagatatct
    30901 gcttctccca gtagaacaag cccgcttcct gttagagcag atgaatcaga ctacacgttt
    30961 gcatctgcag ataaatgaac aaaacgatca aggaatacaa ttttccgatc aaaacaaacc
    31021 tttatcattc aaagtggaaa aatttggaga agattatttg ctgcaagcat tgaatgattt
    31081 tgattttttc tttatgcatt atcagtgggg agttatccaa gggacatttt attctctgac
    31141 gaaatatcaa caaacggttt atcaaacttg gaaacaattg attcgccgat tggagaaacc
    31201 agaagtagtg ttcaagaaaa aagaattacc gtctttgttt aaagagatta tccctctgct
    31261 gtcagagatc ggcgacgtag ctattgcccc tgcagtagaa gaggaagtag cagatatccc
    31321 cgtcgaattt attttctttt tccgaaaagc aaaaggaaaa atccaagctc gagtcgattt
    31381 cgtctatggt gaagtcatct attcgactga cccgaagcat gaggtctcga cagaacaggc
    31441 ttttcaggtg ttacgtgaca cagtagaaga gaaacgagta cttgatctat tcagaatgta
    31501 tcgctaccag aaaaatgaaa ctggttatga acgtccattg ccaagcgggg aagaactata
    31561 tgcctttttc cgcacagaac tatctgtctt ccgtcagttt ggtgaagtca gacttggaag
    31621 aaaattgcga gagttgtatt tagatgcgca taaacatcaa ccgaaaatcg aagtaaccga
    31681 gaccggttca tggctcgata ttcgattcga tgtgacaggg atagaggagc aagaaatcga
    31741 tcatgtcctg caaagcttgc tgagaaatga tgctttttat acattagaaa atggtcaggt
    31801 tctttctttt gattcagaag aatttcaaca aacaagccaa gtgttgcaac aattacggga
    31861 atcgatacgt acagaagaag gaacgatcca tgtacctaaa aatcaaggat tgatcattca
    31921 aaatcaattg gaaaaaagca atgctacttt tagtgaatcg ttccaaacga tggtacaaga
    31981 tctcattcat ccagaaagat accaggcaca gcttcccaaa ggattgaatg caacgatgag
    32041 agattatcaa aagcaaggtt ttcgatggtt gaagatgtta ggtcattatc agtttggcgg
    32101 tattttagcc gatgaaatgg gacttgggaa aacactacag acaattgcct ttttactttc
    32161 ggaaaaagaa gaacggaaaa gcttctctgc tttgatcgta gcgcctgcga gcttaattta
    32221 caactggcag gcagaggtga gaaaattcgc cccttcttta agtatccaag taatcaatgg
    32281 gaataaaaaa gaacgtgaag aattactggc taaagacaca gatatccgag tgacttctta
    32341 tgccagctta agacaggatc tagctaatta tcagtcgcaa aaaattgatt atttgattct
    32401 tgatgaggca caaatggtca aaaatagcag tacgaaaact gcacaagcat tgagagaatt
    32461 agctgtacct cagcggtttg cactgagtgg gacaccaatc gagaataatt tagaagaatt
    32521 gtggtcattg tttgcaacga ttatgccagg atttttcccg actaaaacaa aattcaggga
    32581 acttgccgca gaagaaattg cgcaaatgat tcgaccgttt attttacggc gagataaaca
    32641 aactgtgttg aaagacttgc cggaaaaaac agagatgaat ctatattctg cacttacaga
    32701 agaacaaaaa actgtttatt tagcttactt gcgtcaaatg cgtgaagaaa tcacatcaat
    32761 ggattctgaa gcattcaaga aaaaccggat cgggatactt gctggtctga caagactgcg
    32821 acaaatttgc tgcgatcctc gtctgtttat tgaagattac caaggcggtt ccggcaagct
    32881 cgaacaagta aaagatctac tggtagctgc caaagagaac aaacgaagag ttttgctgtt
    32941 ttctcaattt acagggatgc tgactatctt gcaagaagag ttagccgagc tagggatctc
    33001 gacattttat ttaagaggca gtacgaaacc acaagatcgg ttaagtatgg tagacgcatt
    33061 taatgctggc gaaaaagatg tttttctgat ttccttaaag gctgggggaa ctggattgaa
    33121 tctcacggga gcagatacgg tcatcttgta tgacctttgg tggaatcctg ccattgaaga
    33181 acaagctgct ggacgtgcac atcggatcgg acaaaaaaat gtggtagaag tgtggcgtat
    33241 gatctctgaa ggaacgattg aggaacgcat ggattcactc cagcaagaaa aacgcgaact
    33301 gttccaaaaa gtgatccaag gtaatgaaga acagttgacg aaactgactg aagaagacat
    33361 ccgcatgatt ttaagtgtag gagaaatcga ggagtaagag actcgaaaaa gcgttcagta
    33421 atgggtaaga aagcctttct aaagaagcga ttgtttattt gatttaaaaa atagcaagga
    33481 ggctgggatt ccattttatc ccagcttcct tgctatcatt tttttatttc tatttttaaa
    33541 ggttatacaa agattgtctt cattgtctct aacagctgat caaaagaaga aatctcgata
    33601 tcgcaaattg gagtctgttc aattttgcgt tctcgatggt tgaaccaaag cgcttgccag
    33661 ttagcacttt ttgcacctag aacatcattg tcgtaattat cgccaacgta taaggtctct
    33721 tctggaagca tatgaaattc tttctcagcc agctgaaaaa tctctttttc tggtttttgg
    33781 aaacctgttg cttgagaaat gagcatattg cgggaaggaa tccaattgtt caattgaagt
    33841 tgcttcagtt tttttgtctg atggtcagtc ggcccatttg tgataattcc taatgggaca
    33901 ttcttttcct tcaagaaatc gagggttttt ttgacctcct catgcaagca gatattatca
    33961 agttcttcct cgtaaatttt ttgaaataag aggccatctt cttccgtaat atgagggtag
    34021 tctaaatctt ttagtgattg gctgatccga tgtgctcgca tatattctag tgtccaatct
    34081 cctgccatta cttttgggaa gttttcatca ctatgatggc ggaaccggat atatagtgga
    34141 tgcatatctg catctgaaac aagcggaacg actcttttga ccgcgtttcg aaatggctgc
    34201 tgctgatcat acatcgtatc atcaacatca aacacaactg atttaatcaa aattttacac
    34261 gcctttcggt tttcattgat tttttcacaa atccttgact attgtaacag aaaaaattgt
    34321 ctaaaaaagc tgataaataa aggtttttct tactatattt ctatttcctt gacagaataa
    34381 ggtttttata tttcagtttt tttcactaaa aatcgtgaaa cacgtcaata agtttgatga
    34441 acaaattaat cattatggta gaagtcgatt gatttatttt aatgcaatct ctgtggaatt
    34501 ttcgaaagat ttattttttt aatacagtct caatataact attcaaacat aaaataacgt
    34561 tatttgattt atttgtttat agtaaatttt atttagtaat cattgcttta aaatactacg
    34621 ctta
//
LOCUS       DAZKKW010000023        34602 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.023, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000023 DAZKKW010000000
VERSION     DAZKKW010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34602
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.023"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            214..498
                     /locus_tag="ACG8PO_001077"
     CDS             214..498
                     /locus_tag="ACG8PO_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HFQ0206420.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(779..1198)
                     /locus_tag="ACG8PO_001078"
     CDS             complement(779..1198)
                     /locus_tag="ACG8PO_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HFQ0206421.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKPL"
     gene            complement(1200..1784)
                     /locus_tag="ACG8PO_001079"
     CDS             complement(1200..1784)
                     /locus_tag="ACG8PO_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HFQ0206422.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            complement(1831..3306)
                     /locus_tag="ACG8PO_001080"
     CDS             complement(1831..3306)
                     /locus_tag="ACG8PO_001080"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HFQ0206423.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            3487..3798
                     /locus_tag="ACG8PO_001081"
     CDS             3487..3798
                     /locus_tag="ACG8PO_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0206424.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            complement(3913..6249)
                     /locus_tag="ACG8PO_001082"
     CDS             complement(3913..6249)
                     /locus_tag="ACG8PO_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HFQ0206425.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            6391..7059
                     /locus_tag="ACG8PO_001083"
     CDS             6391..7059
                     /locus_tag="ACG8PO_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFQ0206426.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            7062..8105
                     /locus_tag="ACG8PO_001084"
     CDS             7062..8105
                     /locus_tag="ACG8PO_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFQ0206427.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(8342..9223)
                     /locus_tag="ACG8PO_001085"
     CDS             complement(8342..9223)
                     /locus_tag="ACG8PO_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFQ0206428.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERALRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            9382..10110
                     /locus_tag="ACG8PO_001086"
     CDS             9382..10110
                     /locus_tag="ACG8PO_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HFQ0206429.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKSCGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            10266..10583
                     /locus_tag="ACG8PO_001087"
     CDS             10266..10583
                     /locus_tag="ACG8PO_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFQ0206430.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            10576..11184
                     /locus_tag="ACG8PO_001088"
     CDS             10576..11184
                     /locus_tag="ACG8PO_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HFQ0206431.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            11239..12030
                     /locus_tag="ACG8PO_001089"
     CDS             11239..12030
                     /locus_tag="ACG8PO_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HFQ0206432.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            12168..12656
                     /locus_tag="ACG8PO_001090"
     CDS             12168..12656
                     /locus_tag="ACG8PO_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HFQ0206433.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            12707..13579
                     /locus_tag="ACG8PO_001091"
     CDS             12707..13579
                     /locus_tag="ACG8PO_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFQ0206434.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            13755..14117
                     /locus_tag="ACG8PO_001092"
     CDS             13755..14117
                     /locus_tag="ACG8PO_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFQ0206435.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYIQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            14126..14878
                     /locus_tag="ACG8PO_001093"
     CDS             14126..14878
                     /locus_tag="ACG8PO_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0206436.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            14892..16694
                     /locus_tag="ACG8PO_001094"
     CDS             14892..16694
                     /locus_tag="ACG8PO_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0206437.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            16971..18011
                     /locus_tag="ACG8PO_001095"
     CDS             16971..18011
                     /locus_tag="ACG8PO_001095"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFQ0206438.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(18063..20207)
                     /locus_tag="ACG8PO_001096"
     CDS             complement(18063..20207)
                     /locus_tag="ACG8PO_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFQ0206439.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(20564..21937)
                     /locus_tag="ACG8PO_001097"
     CDS             complement(20564..21937)
                     /locus_tag="ACG8PO_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFQ0206440.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            complement(21937..22332)
                     /gene="rbsD"
                     /locus_tag="ACG8PO_001098"
     CDS             complement(21937..22332)
                     /gene="rbsD"
                     /locus_tag="ACG8PO_001098"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HFQ0206441.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            complement(22360..23277)
                     /gene="rbsK"
                     /locus_tag="ACG8PO_001099"
     CDS             complement(22360..23277)
                     /gene="rbsK"
                     /locus_tag="ACG8PO_001099"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFQ0206442.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            complement(23385..24398)
                     /locus_tag="ACG8PO_001100"
     CDS             complement(23385..24398)
                     /locus_tag="ACG8PO_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFQ0206443.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            complement(24479..25078)
                     /locus_tag="ACG8PO_001101"
     CDS             complement(24479..25078)
                     /locus_tag="ACG8PO_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HFQ0206444.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            25264..25965
                     /locus_tag="ACG8PO_001102"
     CDS             25264..25965
                     /locus_tag="ACG8PO_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0206445.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            25975..28686
                     /locus_tag="ACG8PO_001103"
     CDS             25975..28686
                     /locus_tag="ACG8PO_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HFQ0206446.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            29137..30765
                     /gene="treC"
                     /locus_tag="ACG8PO_001104"
     CDS             29137..30765
                     /gene="treC"
                     /locus_tag="ACG8PO_001104"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HFQ0206447.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(30825..32006)
                     /locus_tag="ACG8PO_001105"
     CDS             complement(30825..32006)
                     /locus_tag="ACG8PO_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFQ0206448.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            32258..33781
                     /locus_tag="ACG8PO_001106"
     CDS             32258..33781
                     /locus_tag="ACG8PO_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HFQ0206449.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKNGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            complement(33850..34578)
                     /locus_tag="ACG8PO_001107"
     CDS             complement(33850..34578)
                     /locus_tag="ACG8PO_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFQ0206450.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
BASE COUNT        10936 a         6190 c         7022 g        10454 t
ORIGIN      
        1 aggaagaggt tgtgagcctg ccgtttaact ctgagtcaca aggagcaatt tcaaaaaaca
       61 gcttctcatg tttgttgact attgtgactt gtgaccgagg agttggcagg agaacaccgt
      121 ttattcgaaa aaaggaactg cgacaagact tttgtcacag ttccttcttt tttttgctat
      181 gctatactag atgtaaaaaa taggagaaat aaaatgaggg aaatttattc ttttgaatca
      241 aaaatctatg cttccgaagt cggcaagggc ggggcatata ttatttttcc gtatgatata
      301 agaaaagaat tcggtgctgg aagagttaaa gtccaagcaa cttttgacaa tgtgccgtat
      361 gaaggcagta tcgtgaatat gggcgtgaaa aatccagatg gttcagtctg ctacatcctt
      421 ggactacgaa aagatattcg taaaaacatc ggaaaagaaa tcggtgatat tgtagcagtt
      481 acagtgaagc aaaaataaaa gaggttgtga caaaagacgt gtcacaacct cttgataaac
      541 ggtgttcaaa agccagtttc tcggtagtaa gtcaaattta tgcaaaaatg tggaaaccca
      601 tgtttacaaa atttttctta ctactcgaaa cgaagcggct ttatcacaac ctcttgataa
      661 acggcgttca atcagctgtc cctcggtatt agctcaaaaa cggcaaaata tgagaagcta
      721 tttttccatt tttttctctc atactcaggt cataacgctg atttcacaac ctcttctttc
      781 ataatggttt ttcaccatct ttataataaa ctggttcttt gatatggaaa tctatttttt
      841 ccaaccaatc taaaatcttg taacctaact cttctgacat cggaaaagag gcgttgattt
      901 gtgcattgga gatatggtca acatggatac cggaagtgat cgtacaactt cccggaacgg
      961 cagttttgat gatcacagct attttttcgg ctaagacatc gtctttatgg aaccgaccat
     1021 cgtggctagg gaaacgtaca gtttgcatct ctgtatcacg agaaaatgta gtgactgttc
     1081 cgatatgtgg tgtatctccg cctgttaaag tgatcaataa gtctttaccg attactttaa
     1141 cagcagcttg gatcgtgaaa cctagtttgg ttacttcaac tatcgtttct ttttccatat
     1201 taatcaccct gccaacgttt tgaatgctca tttacaattt caaattgatc caaaagtttc
     1261 attgtatggt gattaacaag atcttggatc gtttgaggct gattataaaa agcaggaatt
     1321 ggtggtatga tttgcacacc tagtcgtgct aatttagtca agttttccaa gtgaatggca
     1381 tttaatggcg tttcacgagg aacaatgact aactttcgtt gctccttcat tgttacatca
     1441 gcagcacgtg caatcaggtt gtcgcctaaa ccaacagaga tacctgcgac tgttttcata
     1501 cttgccggta cgatgatcat tccatcggtc aaaaatgatc cgctggcgac agcagcacct
     1561 aaatctttgt ttgaataatg gtaatcaaac aggctttcga attcagttag tgataagtcg
     1621 gattccagct ttaagttctg ttttgcccat ggactgatga ttccgtgtgt ttctatcatc
     1681 ggatattctt ttagtttctt cactaggttg atggcataaa tggtgcctga agcgccactt
     1741 acaccaatca cgattttttt cttccgttgt gccaattgtt ccattttccg cactcctttt
     1801 tatgatgtca aaacagttgc agatcattca ttatttcaaa tatttttccc aatcttctac
     1861 ttcttggaat tttgcacgta caaaatgttc tttcatatcg aaaggaactg taccgtcaaa
     1921 gatagttttt gcacttatcc cacggaaacg aatagagcca ggattatatt ctggtaattc
     1981 tgatgggtcc aatggatggt tcctcattcc tgggatcacg atgatatctt tatctccttg
     2041 gaaacgagtg ttgaaagtcc aaactacatc gttcatgtcg aaaatatcaa catcttcatc
     2101 gactaaaatg actgtcttca attctttgaa ggctgataaa gcaagaatag ctgcttgacg
     2161 ctgaatacct tcatccgctt cgttttcttt acggatttgc atgatggtca tcaatttacc
     2221 accacctgca ggaggattgt agacatttac tacttttcct ggaatagctt tatcgaccaa
     2281 ggcaagaata cttgcttctg taggaatacc ggccatagat acgtgttctt cactgggacc
     2341 aatcgttgtt tgcatgattg gattgtcttt tcggtgagta actgctttga ctttcactac
     2401 ttgtacagct ggattagcat ccccagtata gccaggaaat tctggcattg cttttcctga
     2461 gttcgtgttg atatcttctt gcattgtttc atttggcatg atttctgctt caatgatgaa
     2521 ttcagcacga gcaataccca cttcatcgac tgctatacca tcaacgattt gaataggttc
     2581 gttacgcaaa gctcctgcac accatagttc attgtagcca agtggagtgg taggtggttc
     2641 gaaagtcgtg ccaatggcaa ttgctggatc taaaccaata ttgatagtga taggcattgg
     2701 tttattcaag gcttcgaatt gtttttggaa gtgaccaatg tggcgtccgc caggcatgat
     2761 gtacataccg atcgtatctt tatcttctag aaccatacga tggatcgtta catcgctcat
     2821 tgttttttct ggatctgatc ctaagactac acccattgtg atgtatggac cagcatcttc
     2881 aggagtattt gtaggtgcag gcaaaatgtt gcgaatatcg aagtctgcat cggtagctag
     2941 atgaacgact tcttgtgctg gagcgtctgc tttgtctact ttcacaggtt tgattggatt
     3001 ttctacagcg tctttcaaga aacgaccaag tgttcgataa tcatggtgca aaatttcacc
     3061 tacacgttta cgactagcca ttgttccgat gttcacacgt gtgccgggga aacctttgat
     3121 attgttgaaa gtcatagctg gtccttcttg agtcggacgt tttactgtac cgccagcacc
     3181 gatataacgg taaacaccag ataattctgc ttcaggatcg atttcaatat ctgtttcgtg
     3241 atattgacct ggaagtgttt ttagttcttc gatgactttt cttaaatcat acggttgatc
     3301 tgtcatgaga gattcctcct tagttgtctt tacctatcta tagtaaaagg tgaaaaagag
     3361 gaaaacaatt caacttgttc ggtaaagtat tctacaatgg aatggatgcg tttttttgat
     3421 aatagaataa actatgatat acttatcact aaaagaaagc gcatacttag tgttggagga
     3481 aggaaagtga aaaaaatgga ggagtcattt cctaaagcag taaaagtgga aaatatagcg
     3541 aatattttga aagttacatt tgaaaacgga gaagtaaaat acgtgaaatc tcattggaca
     3601 gaagagataa ccgatgcctt gcaatttgga aaaaaaggaa gaggaaaaag aaaaaatctt
     3661 ctcgcacttt ctcgtaatat gtggattggg actgaagtaa cgattgaagc agatggaaca
     3721 gtattcataa atggaaaaga caggtatacg ccagaagagt tgtggtacaa aggaaagaaa
     3781 agtataccag aattataaaa aataattaaa aaaaacagcg cgaaacaagg aacatattac
     3841 ttcctcgttt cttgctgttt tctactattt cagcgctctt atgactcaaa tctaaatgtg
     3901 gttgtcacaa ccttattctg cacgcaaagc gatagcggcg tcttttttcg ctgccattcg
     3961 tgcaggaaga tgaccgccaa gcatcgtcaa tatagtagag acgatcacta gaataatcgc
     4021 atggacagga tttagttgag caacattttt tagatccgtc atgttgtata agatgctatt
     4081 gatagggaac gtagctagcc atgcaataaa cacacctaat atcccagaag aaatacctaa
     4141 aatacacgtt tctgcgtcaa atacgcgagt gatgtctttt ttacgtgcac ctaaagcttt
     4201 tagtacaccg atttcttttg tccgttcaat cacagatgta taagtaatga tactgatcat
     4261 gatcatactt gttactaaag agatccctgc aaaagcaata aggacataag tgatagcatc
     4321 catcaatccg ccagtcagtt cagtcatcgt tccagctaaa tcagtataaa tgattttatc
     4381 ttcatctgat ttgcctttat tgtaatcatc tagataatcc aagatctttt ctttgtcttc
     4441 aaaattgttc ggataaatca tgatacttga aggcaagctg tctccaccta aataactaag
     4501 caatgtttgt ttggcatttt cgtcaacttt ttcagtagtt aagacattta caccactgtc
     4561 tctttgagct ttgacgattt ctgattcttt gttttcgttg acgatttgag tcgttaattg
     4621 atcgctataa gcgattcctg gagataagag attcattgta gaagaagatt tcacgcgtag
     4681 gatccccgag atcttcaatt catcagaagc attctgatac atcgcatcat aatctgtatt
     4741 agggataaaa ttcccagttg gaagttttgt atagtaagta ttgttgttta ctaatttgaa
     4801 tgttgtacca acgatatcat caaaatcaag tttttcgtct tctttcacat caaatccaag
     4861 attcttcaat gcgttgatat tagtattatt attgccgtct acgatcagca cgacatctgt
     4921 tgctgaggct ggataacttc cggcaagtaa agaataattg tcttttaaaa agttttcttt
     4981 gctagtgtca agctgtgtag ggaaacttga cactcccaca cctgtcattg ctgacatcgt
     5041 actagataaa gaaagagatt ccgtatcagg attttgattc gagaagctga caggctgtac
     5101 tttgccattt acatcacgta aaagattgat tcctgtagta cgggtaaatc cgatattatt
     5161 acttaagtta ggatcgatgt ctgttacgta atcgacgtat gtttgatcaa tcttattcgt
     5221 gtgctgtgct ttatcttctt cgctgacttt tgcagtaatc gttttgctgt ctggataatc
     5281 cgctttgctt gcacccaatc cggcatcgtc acgagtctgg ttagtcgtta ccttagaaat
     5341 agtaatcgga aatttcgcca tcgtttcagc ttgcgtgtta tcgatttgtt tttggaatcc
     5401 agaagaaagg gacagaacga tcgcgattcc gatgatccca atactggaag caaaggaggt
     5461 caagaaagta cgtccttttt ttgttcggat attgttgaag gaaagcttta acgctgtcca
     5521 aaaactcatc tttgttctgc gtaagttaaa ttgatcatct tttggtcgtt cgatgtgagg
     5581 atgggaatct gttaagatct taccatcaga aaattcaatg attcgatctg cgtattgatg
     5641 ggccagttca ggattatgtg tgaccatgat cactaatttt tctttcgata actcttggat
     5701 caattccatg atctggatac ttgtttctgt atctaacgct ccggttggtt catcgcacaa
     5761 aaggatatct gggtcattgg ctagtgctcg agcgatggcc actcgctgca tttgtccacc
     5821 agataactga tttggctttt tatgcatatg atttgtcaag ccgactcggt gcaatgcatc
     5881 ttctgcccgt tttcgttttt cgtctttaga aacaccactt aatgtcatcc caagttcaac
     5941 gttttcaata atgcctagat gaccaatcaa gttgtaactt tgaaaaatga aaccgataga
     6001 gttgtttcgg taggcatccc agtcactgtc cttaaagtct ttagtagact ttccgttgat
     6061 taccatatca ccggaatcat aattatctag tccaccaatc acattcagca tagtcgtttt
     6121 accagaacca ctaggaccta aaatcgctac gaattctttt tgtctgaaag cgacagacac
     6181 accatccaat gctttggtgg tggtttcgcc gactttataa tattttttta tatcttttaa
     6241 ttgaagcata atcacccttc tcccgtattg aaatattcgg tatactaata agtatatcag
     6301 aacaatgatc gtagtatacg aaataaatgt taactgaaga tgaactacaa gaaaaaatct
     6361 agaaattttt caaaaaacag agggagatca atgaatcgta ttttagtagt agaagatgat
     6421 ccgcaattac agttgcttta ccgttctgtg ttggaacgag cgggcttttc agtcatcacc
     6481 gcattgaatg gagaggatgc gctgaaaaaa ctagaagcga cacgtataga attaatcatt
     6541 acagatatca tgatgccgaa aatggatggc tatgacttgc tagactcatt acgtagcacc
     6601 agatttgaca cacctgttct aatgattacg gcaaaggccg attttgagga taagaaacgc
     6661 gggttcaaat taggagcaga tgattatatg acaaaaccca tcgatgtcaa tgagatgatc
     6721 ctacgtgtgg aagcattgct tcgacgttca aagatcaatt acagcagtag cttgataatc
     6781 ggacaaacaa aattagagct agatgcttat acagtgttac aagatcagca ttcgttgatt
     6841 ctgcctcaaa aagaatttct gcttctgtat aaattactgt cttatcccaa caagatcttc
     6901 actcgccaac agttgatgga tgatatttgg ggattagata cgaatacaga agaacgaacg
     6961 atcgatgtac atatcaaacg tttaaggacc cgttttgaaa agacggaaga ttttcaaatc
     7021 atcacagtac ggggactagg ctataaggcg gttatttaaa gatgaaaaaa aagctatttt
     7081 cacaattatg gatctatttt gctagtattg tcttcatttc gatcttagtg acactgctgt
     7141 gttttctcgg ttttattttc ttattgtctc gacattctat ctcgcctgct gaatcggctg
     7201 gaaaaccaac gttatacccg ttattcgtct ttgcggggtt cagtatggtc gtgggtacag
     7261 gaatatcgat ttttgttggg cggcgtatcc tgcatccaat cagtgcgcta gggacaaata
     7321 tgagtctggt agcaacggga gatttttcga ttcgaatgga tgaacagcag aaagtagcag
     7381 aagtacagca actgtataaa gatttcaacg ttatggtcca agaactcaat agtatcgaga
     7441 cactgaggaa tgactttgtg tccagtgtat cccatgaatt caaaacacca ctagcaacaa
     7501 tccaaggata tgttcaattg ttacaagccc caaatctctc tgacgaagaa cgccaaatat
     7561 ttttatatcg gattatcgaa agtatcacgc agctttcaca attaacagag aacatcttaa
     7621 aattgaacaa actggaaaac cagcgtatcc aactggaaaa gaaagagtat cgattagatg
     7681 aacaaatcag agaagtaatc gttttttttc agcctaaatg ggagaaagaa cagctagaat
     7741 tggatatcga acttgctgca gtaaattaca caggcaatga agagtttttg taccaagtgt
     7801 ggctgaatat catggataac gccataaagt acaatcaaat caatggacag attcatatca
     7861 aactatttga gacagccaca gagattgtac tggaagtgac cgattcaggt gtaggaatga
     7921 atgaagagac cagagatcga atgtttgaaa aattttatca aggagataca agtcgtcaaa
     7981 tttctgggaa tgggctagga ctttcactgg ttaaaaaaat tctagaactg catgatggac
     8041 gtattgatta tagcagtatc gaaggagtag gaacgacagc aatgatacgg ttaagtaaac
     8101 aataggaaaa agaataaaac ccctaaaaat tctattgaag gtttttaggg gtttttagtc
     8161 gtttttttgt gattagggaa atgttcttac ggttcttttg gcataaaaat cgctgctttc
     8221 ttattgaact acgagaaatg ttagaggtgt cattgaaaca aggacatgat gcaagaaaga
     8281 gagcgaaagt catgcgtcgc tctctttttt tgggtggata gatggacaga aaatgtaggg
     8341 cttagttttc ttcttgattt aaatactccc aaaatgtatc tgaagccaaa gaatgctcac
     8401 cgactttacg agtaaatgca agataactct ctgttgttgg agaaataggg agaatcacag
     8461 tattttcttt taatagattc ttgactgttt tttccataac aatagcaatc cccatgtcat
     8521 ttgcaatcat attgatgatc aagtcgattc ttgctccttt ataagagatt tttggttcaa
     8581 atccagcttc gcgacacaga tctagtacag gttgatacaa gttcgtagtg gggccaagga
     8641 gcaaaaagtt ctcctttttc agttcgctta gatccaattt ctttttacta gctaaaggat
     8701 gatttttagg tagaactgct acaaaatcgt ctgtttcagt aggcaaccac tcaatcgaac
     8761 ttggtggggc ctggaaactt ctagcaaagt ggatcgtgtc actatgttca ttggcgtcat
     8821 tgtaagttaa ttcgacactt tccacttctt tcaactggac tgtaaattca ggatggcgtt
     8881 tcaaaaactc ggtgattttg gaaaaacctt tataattgat cattgtcggt atcgttaaga
     8941 tatttaatga aagattgttg tctttcgtat aggtttcaag cacttcttgc atagcatggt
     9001 attgttctac gacatttttt acatatggga gcaataagct tccagcttcg gtcaattcga
     9061 tttttctaag ggcgcgttcg aataatttga cgtctaattc tgtttctaac gaaagaatct
     9121 gtttagaaat atttccttgg gtggtgaatt gtcgatcagc agttttggtg aaattcattg
     9181 tttcggctaa atcaagaaaa acttcgaatt tatggatatc catcatattc ctcctactta
     9241 ttccaataaa gaatacttta acacaaaaaa tgaattggtg taatcttctc tgtctgttac
     9301 aatctttctt gcagcgaaag caataattac agaaattttt ctgatttttt tcgcttcgta
     9361 gaaaaagaga ggagtttttt gatgccaact ggagttttga tcaatgtagg ttccgttttg
     9421 tttggcggac taattggcgg attagtagga aataaattaa gtgaacattt taaagcacag
     9481 ctgacgatgg tttttggtgt gtgttcgatg gggatgggaa tctattccat tgcgccgatg
     9541 aaaaatatgc cggctgttat ttttgcttta gtgataggaa cagcgattgg tttgattgtt
     9601 catttaggta atgggataaa taagggggct gcgctgatgc aagtacctat ttctaaaatc
     9661 ttccctagtg aaaagcttgg tatgacccac gatgaattta tcagcacatt agtgacggtc
     9721 attgttctat tttgtgcaag tggtacgggg atctatggaa gtttagattc tgggatgaca
     9781 ggagacagta cgattttgat ttctaaatct gtccttgatt ttttcacagc agctattttt
     9841 gcgtgtaatt tagggtatgt cgtttctgtg atagcaattc cccaattcat tattttttat
     9901 attttgttct tattggcaaa atttatttat ccattgacta caccagacat gatcttagat
     9961 tttaaatctt gcggcggatt cttgatggtc gctaccggtt ttagaatgat caacgtgaaa
    10021 atgttcccag tagctgacat gatacctgca atgatcgtta tcatgccact gagctggatg
    10081 tggacaaatt ggattatgcc actgttataa taaaaatcaa agagaagcca ttttttgctg
    10141 actaaaaagg atcaaatctt ttagtcagtt tttttgtatt tgacaaactg attttgaaac
    10201 tatattatat gttgtataat atagattgaa ataaaatatt acacttgaga atagaaggtg
    10261 acgtaatgca catccagatt ccaactgttc ttcttgatgg tactgtgcta gcagcattaa
    10321 gcaaggaaga tatgtatggg tatgccttaa caaaaatcgt acaagaaagt ctctctgtca
    10381 gtgaatctac actatatccg gttttgagaa gattgaaaaa agaaggatat cttgagacct
    10441 atgatgaacc ttttgaaggc agaaaccgtc gatattactc gttgacacca atgggaagtc
    10501 gtcatttaga agaaattatt gatgaatgga ttgtttttcg agattcttta gaccggttat
    10561 tggaggagag cacaaatgaa tgaatatttg aatgaagttg tggatcatct tcaagaaatt
    10621 cctgaagacg atcgttttga tttgatccag tattatgagg aatatttttt agattctgga
    10681 aaaacattag atcagatcat tgatgaatat ggtacaccaa agcagtttgc cttaaaatta
    10741 aaaatcagtt acttttcaga agaagaacaa gcagaaaacg agttagtgaa aaatccaaaa
    10801 agacaaattc gcctgatttg gttaatcatt atcggtttat ttgcttctcc tgtcctcatt
    10861 ccactagcac tggggtttat attggttatt gggggactat tggttgcttt actagcgctc
    10921 atttttagta tctatgtttt atgtatcagt atcttaggtg ttggcctatt catgatcatc
    10981 tcaggaatca gtgtgtttgg acaatcgatt ctgagtggat tattctttat cggtgtcggt
    11041 ctgttcgcca caggaagcgc agtatttatt tcgccactgt tgctgaaagg aacaaaaggg
    11101 cttttttatg tcatgatgaa ctttgttaaa tggatgggac gacgttttat tacaaaaaga
    11161 gatctgcatt cagcgggcgt ttgaaggagg acacataaat tgagattgaa acattttttg
    11221 attaccggta ttggtctgat gcttactggt ggcctcctag caggtgcaac ttatacatta
    11281 ggcgctcaaa agagtctgac ttgggataat ggtcctcggt tgatcgatat ggttactgag
    11341 acaaaaaaaa tcaaggatgc aaacaaaatc gtcatccatg cagaaaatca aacagtttcg
    11401 gtcatacgcg gattggattt tgagataaaa acgacttatg ataagacaga aaagccagaa
    11461 ataaaggaga aaaatggcgt gttgactgtg aatgcagtca aaaaatcaac aggtgcaatc
    11521 attagtttag aagacacaag tgcacaaatc actattgttg tccctgcaga tcatgaatta
    11581 tctgaattga caatcgaagg gaataatagt gcaattgata tcaacgaagt atcaatcaaa
    11641 aaagtgaata ctaagacaca tgatggctgg gtatcgttca atgaatttac aggaggaaca
    11701 ataactgcga aagatgaatt aggctcactt cgattagatg aggccaatgt ggataacttg
    11761 atcgtcacag gtgaaaacag caacttccat ctagaagaca gtttgatcag caaagaagga
    11821 aaagtcatcc taaacggtgg caatttatct ttgcatgaga tggaaaacag tggctatact
    11881 atccaaacgt ctggtgaatc ttatattgaa aaagactacc agccaataac aaatactttg
    11941 ttagaacaag gacagaaaaa gctaaagatc actgctaaag acacatcgat caatattacg
    12001 acggatcaag aggacaatga aacagattga tcctgtactc aactggatat tttaagaaaa
    12061 aacagcaaag atccttccaa tctttgctgt ttttcatcgc ttaacgcaca taactatagt
    12121 aaaataagaa agaaaatatc taaaaagaaa acagggtgaa agagtatatg aaggtagaag
    12181 aaggtcaaag agaaaagtta aaaacagaaa tggaaagatt acatacttat atcactcaac
    12241 taagccaaac cttctatgat ccagacaaag aaaaagtgat ggttaattac ccgaacaatt
    12301 cagaaggtcg ccaattagag caagtgtacc atgaagtttt caaacattta ttgacggtga
    12361 agaaagagct ggattattat tctttaccga ttatcgatac aggaatattg aagtatgatg
    12421 ggaagaagga acgatttatc tttaaatctg ttcgggaaga tttgcctctt tcagcaggga
    12481 tggatctaga ggtcttggta gaagactatt ttacagaaga aaagcactgg gttcgaacaa
    12541 aattggatta tttaccgcag gcagccggag ggacacaggc aagcggttgg tacatcactg
    12601 aagacaaaga gttagaacta gaaggtgtaa tggcgcgtat tcgaaaaaaa aattaaaaaa
    12661 gtaacattag atatttattg aaggataaag gagcgcagga agtagcatga gagaaaaaag
    12721 agcattttat atcaatggtt atgcagggat tgtcggattg gtcgtattag ctttagttgg
    12781 attgtttttc ttctatttag gtatgtggca agcgaaagtt tgggcacttt ttttaagtat
    12841 ttttctttgg ttgatcacgt tattgttgtt aagttcggca actgtagtca gtcctaatca
    12901 agcaaaagta attttgtttt ttggacaata tttaggtacg attcgagaaa atggcttttt
    12961 ccttacgata cctttggcac aaaaaatgac tgtttcgttg aaagttcgga atttcaatag
    13021 ttctgtttta aaagtcaatg atttggatgg aaaccccatc gaaatctcag cagtagtagt
    13081 ctttaaagtc gttgatacag caaaggcatt attcgatgta gcgtattatc aagattttgt
    13141 agaaatccaa agtgagacag ccattcggca tatcgctagc caatacccgt atgatacatt
    13201 caatgagaat gatttgacgc ttcgaggaaa cacgacggca gtgtctgacg agttgcaaaa
    13261 agagctgcaa gagcgtttag cagtagcagg tgtcgaagta atcgaaacac gcttgaacca
    13321 tttagcctat gcaacagaaa tcgctagtgc catgctccaa cgtcaacaag caaaagccat
    13381 tttgtctgct cgacaaacca ttgttgaagg agctgtcaca atcactcaaa tggccttaga
    13441 gcaaatcgaa gatggacaag aaatcaattt caccgatgat cggaaagtcc aattgatcaa
    13501 taatttactt gtctcaatca ttacagacaa aggaacgcaa cctgttatca atacaggcga
    13561 catgaaagac cgtaagtgac atactttttg taagatagga agaaggagtt taggacggat
    13621 tattgtcttg aactcttttt ttagttgttt taaagaaaat gacaaaattg aaagaagaat
    13681 gatagttgat tcgaaggcag aattgttcgc ttttttctat aatcagtata taaagtaaga
    13741 gagaagggga aaaagtgaaa aaacttctga tcggtctact tgttttcatc gttgtggtac
    13801 ttgcaggggt gaaaatcgca gattacgtag ttatgggtgg agaagaatac tatatacaaa
    13861 ttacgacaga tggtacaaaa acagtttcaa aatcagactc tggacaagaa acagtcaact
    13921 atagatatat cctgcctggc tatacgaaag agggtaagga aaaagaactg gacttcaagg
    13981 gacaattgac tcgaccgctt cgtaaaggag catatttaaa aatcacctgg aacaaaaata
    14041 aaggtgtcac ttcgtatgaa gaagtcgaaa aaaaagacgt gccgaaaaca gcactggaaa
    14101 aactaattaa agggtaggga aagaaatgaa caaaatagtc gaagtacaga atgtaacaaa
    14161 agtatacgga aaaaacaatg agaaaaagac gcaagcatta agcggcatca gcttcgatgt
    14221 agaaaaaggc gaatttattg ggatcatggg cgcttctggt tctggtaagt cgacactttt
    14281 gaatatttta tccacattag ataaaccaac agatgggcat atccgaatca atcaaaaaga
    14341 tgtgaccaca ttaaaaggaa atcaactagc agattttcgt gcgaatgaga ttggctttat
    14401 tttccaagat ttcaatttat tagaaagctt gacagcacaa gaaaatatcg ctgttcctct
    14461 ttctttacaa ggcgtcagac caaaagaaat caagcaacgt gtccaaaaaa ttgcagaacg
    14521 tttatccatc tctcatattt tggaaagcta tccttctgag atttccggtg gacaaaaaca
    14581 acgggtcgcc gccgcacgtg cattgatcac acaaccaacg attctattag gggatgagcc
    14641 tacaggtgcg ctggattcca aaagtgcccg ggatctttta gatacgatgg acgaattgaa
    14701 tacaaaagat catgtatcga ttcttcttgt gacgcatgat ccgttttctg caagttattg
    14761 ccaacgtatt ttattcatca aggatggcgg tatccatcaa gaagtgaaac gtggagatca
    14821 aagccgtgat tccttctatc gagaaatact atctattctt ggaaacttgg aacagtaggg
    14881 ggagattgac tatgctttgg aaattatcac ttactgggat caagagccgt ttaagagact
    14941 acttggtgtt attctcagga ttagttatgg catctgctat tttttacatg tttgaatcaa
    15001 tggcaagcaa cgaagctttt ttgaaaagta atacgatcgt tgcggctaca gttatcattt
    15061 tccgattcgg ttctgtttta ctagggatca tcacattcgt ttatatttta tatgcaaatt
    15121 cctttttgat gacgatgcgg caaaaagact atgcaatgtt tatgatgctg ggagcaaaag
    15181 gcagaaaaat cgcacagatg attttttctg aaacattctt agtagggatc gcagctactt
    15241 tagtgggttc agctgtcggt gtaggattga caagtatcgt ccaccgatta ttagtggatc
    15301 agttagatct caatatttcg cattttacac cttttagtat tcacgggttg ttgatcactg
    15361 ttttgttttt tgctgttttg tttttattag ctgctgttgt caatgctttt tcgattgtca
    15421 aaaaaccgat tttagcattg attcgtgcgg atcagacacc aacaagaatc aagcagcata
    15481 aattcttgtt tttccttgaa gtagtactgg gaattgcttt tctaggctgc ggttactata
    15541 tgatgaaaca cgtggggacc tatcaagtct ttggtatagg ggtagcctta gttacgatcg
    15601 tattaggaac ttatttcatt ttccattctg tgatcatttt ctttttatca ttactaaaga
    15661 aatcagaaca gatttcattg aaaaaattga acaatttcac attgtcacag ttaaactttc
    15721 gtattcgtga atatacacaa atgctctcaa tggtcgctat gctgtttgcc ttggcactgg
    15781 gcgctttgac ggtaggatta ggttttaaaa atgaaattgc agacttgaca gaaaacaatt
    15841 ctggatatga tctagtgatg aataatcctc gggcagaaga tcagcaaaaa atcaaagaac
    15901 tttcaccaac actttctgct tcttatactc aaaaagaaga tgcgggaacg atttactata
    15961 atgcaagtga atttgcggct caaccattga tcatgataaa acatgaagat ggaacaccgc
    16021 cgaagatcac gaaagaaaaa gttcctgcta aaaaaatgaa tgaactgact gtacaagagg
    16081 aacttcgaag ctatgaattg ccggaacagc aagaaaaaga aatcaaggta gtcagtcaat
    16141 cagaatttga tcaactgaac ttgccacaat ccactttaca gctagttcag gtaaaagact
    16201 tccaagcaaa cctgaaacca atcgaaacac ttgttgctca aaataaaatg aacaatccat
    16261 ctttgcaaaa agtagagtac agcaaccaga aatatgaaat gtatgaagtg tataatggtc
    16321 tattctctgg ttttgaattt atgggattct tcctaggttt agctttcttg accatgcttg
    16381 ctagctgttt gatgttcaag atcttgtcag gaagcaagag tgacattgct cgttatgaaa
    16441 tgcttgaaaa gataggaaca cgacgcgggt tgcttaaaca atcgatccgt agagaagtag
    16501 gcgtgttatt cttagcacca ggaatcttag gtgtagtcca tgtcttattc ggattacaaa
    16561 tgtttggtct attcatgcaa aatccataca aagatatttg ggttccgttt acgatattct
    16621 tcgtattgta tttcatctac tatgtactga cgacatggtt atatactgga atcgtattga
    16681 aaaagagatt gtgaaaaagc tgtcctgcat caagtaataa gaacagccaa gcaaaaatag
    16741 ctcctcatat ttttcgtttg gttgggctta ttaccgcaga tgcaagcttt tgaacaccgt
    16801 ttgttcggaa aaagaggttg tgacaatatt tttgtcacaa cctttttttc acggtctttt
    16861 attagaatat agtaaatttt ttctcgttac tcacggaata agctgttaag aaaacggttt
    16921 ccatgtatac tggaatcagt gcaaatgaaa gaaaagaggg tgcaaggaac atggaaatca
    16981 atgaacaaaa ttttggcgaa aactatcact tgattacgat cacgaatcaa aatgggacat
    17041 ctttgtctgt cagtgatctt ggggcacggg ttgttcattt tcaaacaata atcgataatg
    17101 aaaaacgaga gttgatctta ggttttgatg aagcaaaaga atatctagaa aaagaccctt
    17161 atatcggtgc ttcaatcgga cgtatggctg gacggattac gaatggcaca ttcaaattag
    17221 atgatgaaac gttcgaagta aaaacagacc cggacactgg tcattgttta catggtggtg
    17281 cacctagctt tgaagccaag cagtggcatt atacagtcat caatggtgaa aacgaagcat
    17341 cggtcatttt ctatcagact agcccagata tggaaaacgg atttcctgga actttagatg
    17401 tagaagtacg gtatacgctg acgaacgatg atacatggcg agtgacggtc caagggatca
    17461 gtgaccgcaa aacagtattc aatcctacaa atcatgtcta tttcaattta accggtgatc
    17521 cagcacagtc aatcgacgaa catatacttt ggttgaatag ccgattattt gctacgctca
    17581 ataaagatac aacgcctaca ggagaagtag tttcagtaag tggcacagca tttgacttcc
    17641 aacgtcctaa aaaattaggt gaggtctttg ccagtgattt tcgccaatca atgatagtgg
    17701 gcggcatcga tcatccattt ttgttgaaaa aacaatctgg tttactaaat gctgcggaat
    17761 tgacaagccc agatgaaaaa gtatcgatcc aagtaaaaac agatgcttca ggaatcgtga
    17821 tttttacagc aaattttgga gaagatggac cggaaatgcg cgggaataaa ttagtaaatc
    17881 atggcggtat cacatttgag actcaagaac tgccaggagc ggagcgactt gaaagttttg
    17941 gagatgtcac tctggaacct aaccaagtac gcgagtttgt aacggaatac aaaataaata
    18001 taaagaaata gaataaatta aaaagggagt gtgacagtgt cacatcccct ttttaattta
    18061 ttctatttct ttaaaggcag atcaagccag aatgatttgt ctaaacttaa caaggttagt
    18121 acaggaacag ttgcagcttt aactgtacga gtagttgttt ctggtatctg tcttgccgta
    18181 aaattgttcc acaaatccca gattgaagcg acacggaaga tggaagaagc gttgttgacg
    18241 cgttttatcg ttgaaaattc taatcctgtg attggcacgc catctgttgt ccatttgatt
    18301 ggcacatcga atcgcttaga catattgagg atttcagcgt ttgcttcttc agttgttgct
    18361 gcttcttttt tcggttcatc atattttgtc aggtcgtacg cttcgatgat cgcgttcaat
    18421 ttttgtgaat attggcgatc tgtagcataa cgtccttcca aatatttggt tgcttctttg
    18481 tagtcttttg tttccgactt ccaagttggt ttgtagaaat caggattgcc agtcagtcca
    18541 tttttgatca agtcggcata gtcttccaaa ctttccttgt aagaaggata tttacggaat
    18601 tgtgcgcgga tttggtagct ttgtccggaa ctatcttgtt caagtgtatt gaaggtcacg
    18661 ctgcttcctt tatatgatcc tttgatacca aaaaggttat gatttggttc gcttgataat
    18721 aagctgtttc cagatccact ttcaaggata gcttgagcca tcatgactga tgcgtagatt
    18781 ccttcttttt gcccgatttc ctgtgcatca tctgcaatct ttttcaagaa ctgatcagtt
    18841 gtttggtttt gactgaaatg gaacgagcca tctaatccat ctaaaataga tgagccgcct
    18901 gtcagcgaac ctgaagcagc aggtaaacct gctaagttta ctcggacagc aaaatcaggc
    18961 gtgaaatctg aaatagaagt tactcgaact ttatctcctg gttgaggcgc atggataaat
    19021 tgtccgttcc caatatagat acctacatgg tgagtaacac cggaagaagg tccccagaag
    19081 acgaggtcgc caggttgggc ctgacttact ggaatctgcg tacctgcata ttgctgttcg
    19141 cctgtccagc caccgatata atgaccggtt gctttcatat atacgtaata agtcaaacca
    19201 gaacagtcaa aagtatttgg accttttgct ccccatacat atggttttcc aagatgcttg
    19261 aatgcttctc gaacgattgc ttgttggaca ttcgttgtat ttgtaggcaa tacagcatca
    19321 attggtgatt ttacaacaaa atcagaagaa gcagaagatg gattctctat accgctttga
    19381 tttgctgtat ttggacgaga tgaagcattg ttcgttccgc cattcgatga accattgttt
    19441 ggatggtttg gttctctctg agtatttcca ttgttattcg atccagatgg gtttgtttga
    19501 ttcgaagaat tcccgctgtt cgaattattg ttattattgt ttgaattatt tgaaccatta
    19561 ttattgttgt tattacccac atctggtttg tttggcgttt ccggatctgg tgtactaggg
    19621 acttcttcac taggatcagt tggtttctct gttgaagtat ccggtccagc tttaaattct
    19681 gcagtaatag ataaattgtt cgtacctact gtaaaggtct ttgcatcata atttacttct
    19741 ttggcaccat taacagcgac tgaagcaatt tgttcacctt cagctggcgt aatagtgtaa
    19801 gttaccttcg tttccgctaa taaaccttct accgttccac tttccgtaat atccaaaggt
    19861 tgctgttcag gggaatccgc tgtcgaagca gtcagttgga tcttaccaat caaagaagga
    19921 gcaattgtaa gggaatatgt ctcggctggc ttttcatttt ctgatggatc agccggatca
    19981 atttcagttg gctcggtcga tcctgttgtt gagttagttg ttccagttgg gtcttctgta
    20041 aaagaatccg ttgtttcact agtagctgag ctggatgtag tcgtatctgt tgtgactgtt
    20101 tgatcagcta tttgattatc tacggcaaat acggtattgg tcgacaaaac agacggagca
    20161 atcagccaag tagcagctag caaacgtatc gtttttttat ttttcataat aaactagtcc
    20221 ttttcctttc attgattttc tgtcttattc agcattcatt agacattttt tggaacagat
    20281 aatcgtgtcg aaaagctttt tcttttcata aatctgtctg tgtattctaa ataaaacaat
    20341 ttgtaaataa gcaacgagca ttcattcgtt tttctttgac gttcatttta ttgcatagac
    20401 cacctttgtt tggggataaa caagtgggga taaaaatggt tatgcatatg taaattatac
    20461 caaagtttgg aaagtatcac aaaaatttat aaaaataatg atcctaaaaa gaaaaagact
    20521 gaaatatgtg ataaacatct catacttcag tctttttcat taactattca taagacatta
    20581 ggtcttcatc aaacccgcta acgagagtag tgatcattgc tacgactaga ctaacagcca
    20641 gtcctgctaa gtaccagaaa aaagtaccgg aaatgaatgc aggcaatgtg gtcagactac
    20701 caaatacaaa agcgatcgct ttcacttggg caaatcccat tacagctcca ccagcagctg
    20761 caccaatgca agcagcaaca aatggttttt tcaatgggat cgcaattccg taaacagttg
    20821 gttcggtcac tcccgcaagt aaaatcgaag caaaagcaga accggagatc gaacgggttt
    20881 ttgaacgttt acttcgcaga aatacaccta aagctgcacc agccattcca aaattggctg
    20941 ctcctgtcaa cggaacgatt cggtcgaacc cataagcaga gatattactg atcatgacag
    21001 ggtttacagc ccattggata ccaaatatta ccaaaatatt ccaaccacca gcgatgattg
    21061 cgccactgat cggtccgtta atatccatca tccaagtaat tgcggcagct aaaccttcgc
    21121 caaggtaaac acccgcaggc ccgaatacca ttaacgtcaa aggaaccgtg ataactaatg
    21181 aaagtaaagg tacaaatact aattgcatat tttcgcgaat atattttttg aggaattttt
    21241 caacaaaaga ataaaagaaa atacctaaaa tcgccggcat gacagtagac gtatactgca
    21301 tcagtactac aggaatcccc agaaaatcag tagctgcttt gccactgtct gaaagtaaag
    21361 aggtcagatt cggttcaatc agtgctgcag caatgacagc cgcaatatat ccattcactt
    21421 taaacgtttt ggcagcagaa aaagcaagaa agattggcag aaaataaaag acggcattcg
    21481 acgcagcaga caaaatatga taagttccgc ttgtttcact aagccagcca atcgtagtcg
    21541 ctaaaacaac cagacctttt aatataccag aaccggcaaa cagcggaatg atcggcgtaa
    21601 agatttccga tatcaaacca aatagttttt cgatcggatt tagcttttct ttcggagctg
    21661 aatgatcgat ttgacgcgtc aattccttgt aagcaggagc caatccctga tccatgatta
    21721 tttctgcata tctatcgagt aaaatgaaat acttaatgga agggatttgc tgttttaatt
    21781 tttctgcatt ggctactggc tgttttttca atttgatacg aagccgagtc gcacatggaa
    21841 ggacagaatc aatattgtct tttcctccca actgttcaat cagcatatga atctgctcgg
    21901 acagtaccat ctgttcagga ttcattgtcg tttccattag aagaccactc cactcactaa
    21961 tataatattg gcgtaaggtg tcatttcgcc tgtacgaata aatgcatgac agttattcaa
    22021 ctcttttttc atttcttcat gtgggataaa gctaatcggt gtatctggta acaataattt
    22081 gattttttcc aatatttccg ggttcattgt ctttatttct tccgccaatt caatcgattg
    22141 tacctcaagt tcttctaaaa cgttttctaa aacagataaa aaacttggaa gaccgtttga
    22201 gacagccaaa tctatttttt cagtaccaaa ggggacaggc atgcctgcat ccccgatact
    22261 taatttatca aagtgcccca tttgtgcgat tactcgtgaa atatctgaat tgattacttt
    22321 tccttttttc atgtttgttt cttcctctca ttgttaattt tagactgctg attgttctaa
    22381 agcttcgcgg taagggatag aaggttgcgc accataagac tgcacagcaa cagaagaggc
    22441 tagatttcca taaaggatcg cttctttcaa gttactaagg tctggctgaa gtgtgctgct
    22501 taatgcgcca ataaatgtat cacctgcggc tgttgtgtca acggcatcca ctttaaaagc
    22561 agggacgata ccgtgttctt tttctgtatg atagaaagcg ccggcactgc ctagtgtaat
    22621 gattactgtt ccaataccta attcatggag tttttcggct gctgcgacca aactgttatg
    22681 atctgtcact ctgattcctg taatgatttc tgtttctgtt tcatttggta taatgatatc
    22741 tgtcttttca agtagttcag taggaatcgt ttcacgagct ggtgcaggat tcaggatcgt
    22801 tgtttttcct gcgtcttttg caatagaaaa agctaggatc gtcgcatcta agctactttc
    22861 aaactgagca ataataaagt cactgtttgt aatcagttgc ttgtttttta ggacttcttg
    22921 ttcatggaaa gcattatttg cccctgcatg gatcaagata ctattttctc ctgctgcgtc
    22981 taccataatc attgcttgtc cggttgtggc gccttggatt tcagctatcc cagatgtatc
    23041 gatgttttct tttgtcagta gatctaataa ctgttgtcct tcgctgtcag ccccaacgcc
    23101 tccaataaaa gatgtccgtg cgcctgaacg tttggctgcc actgcttggt tggcaccttt
    23161 acctccgcca gcgtgaaaaa tctcatgcgc atgcatcgtt tcgcctggct tgggcatgtt
    23221 ggttaaacga agggtagtgt ccatattaat acttccaatt actgtaaccg tgttcatttt
    23281 acaaacctcc aactatctca tttattttga tgggaaaatt attttttaaa acgttttagt
    23341 aaaacgtttt aatagttcga taaaaatata gcacaccttg aaagctattt caagggtgca
    23401 gttgaaaatc ttttttctaa tcgtacaggt aatctttttt cttcccattc tttttctggg
    23461 aattggatcc gatctaacaa taacttggca gatgtttgtc ccaattcgaa gattggttgt
    23521 gcgatcgttg tcagtttcgg cttgatatac tcgcacatat ctatattatc atagccaata
    23581 atgctgtagt cttcagggat gctttttcca gcttcctcta atccgcgata taatccaaaa
    23641 gctaattcat catttaaagc aaacacacca gttgcagcag aatcaagaag ttctgctgtg
    23701 atctggtagc caccttgttt tgagaactgt gtaggtaaga ggatcagttg gtcgtgaggg
    23761 atttggtaaa catcaagagc agatttaaac ccctcaatcc gagcgtggac attttccggt
    23821 ggattttcag gatagacaag ggcgatcgtt tgatgtccta gactaagcaa gtgcatgccc
    23881 gctaggtaac caccgcgaaa atcgtctgtt cgtacggcat cactaaatcc ttcagatttt
    23941 ttctgatcca aaacaatgaa gggccggcct tgttttttca aattttcgtt gattgcatct
    24001 gtagagacag cagaagtcgc gataatgaat ccatccacac ctcgacgtgt gagctcggct
    24061 agatattcga tttctttttg atgatcagaa tcagcattac ataatatcgt gacgaaattc
    24121 tgcttataca agatatcctc aattcctcgc atcagtgtac tgaagaatgg attcgtgata
    24181 tcaggaacga gtacaccaat tgttttcgtt tccttggtaa tcatttgtct agcaaaatag
    24241 tcaggctgat agcctaattc ttctttggca gctaaaactt tctgaacagt tttcggactg
    24301 aatttctccg tatttccatt taatatcaag gagacagttg tgattgaaac gccggaaagt
    24361 ttagcgactt ctttgattgt gatctttttt ttcaccatgt tcatactcct atctaaatcc
    24421 tgtggttatt ttcccatgaa atcaaaaaga agtaaatgaa aatcacgtgt gccagtactt
    24481 atttgctttg ttggatagct tcgattattt gtttcgttga gcgtactcgt gcagttttcg
    24541 gaaatatctt ttttatgaac aattgatggg attctttgtc acgatcactg caagcatctt
    24601 cagcaagcac gatatggtac ccgtgttgaa aggcatctaa tgcagtagca tatacaccgt
    24661 ttgttgtcgc tacaccgctt aaaatgatag tttcgattcc acgccggcga agttgaagat
    24721 caagagaagt accaaaaaaa gcacttggat tgtattttgt gatagtcaac atattatctg
    24781 tatctttcaa agaatccttc aaaagtaaat cagtgaattc tggaggtaca gaagcagtca
    24841 ttccacccat atcagtttgt tgactcaaat aagggaaagt ggagatgtct acgttcacta
    24901 aaactttcaa ggcagcggta cgtttcaatg ttcttactaa caaatcattg tttgtcagaa
    24961 tagatgctgc agtgtgtgga gagagtgtac cgttatttac gatctctttt tgcatgtcga
    25021 tcgcgacaaa agcagttttt gataaatcaa aaatcatatt ttcctttaaa tcattcatga
    25081 tgttccttct ttcaaataac aatgtttttt atgtaagtaa atcataaatc gtttatttgt
    25141 caactaaatt aaaggattcc tttctttagt ctagagaaat tcagtggtaa aataaataag
    25201 tgtgagaaaa cgcattcgat gcatagatat agcgagaaat atcgtgaaaa tggaggaatc
    25261 agagtgaagt atgtcgaagt aaaaaatgaa tacaaacgat acaaaatggg agaaacgacc
    25321 attacagcaa atgacgatat ttctttttca attgaaaaag gcgaattagt gattatactt
    25381 ggtccaagtg gagcaggaaa gtcaacgatt cttaatattt taggtgggat ggatacacct
    25441 gatgaaggtc agatcattat tgatgacaca gatattgcgc aattttcaga taaacagctt
    25501 actgcttacc gacgaacaga tgtagggttc gttttccaat tctacaatct cgttcctaat
    25561 ctaacagcaa aagaaaatgt ggaattggca acggaagttt ctcctgatgc gcttgatcct
    25621 gtagaggtat tgaggcaagt gggcctagct catcgattga ataactttcc gtcccaatta
    25681 tcaggaggcg aacagcagag ggtttcaatt gctcgtgcac tagctaaaaa tcccaaactt
    25741 cttttatgtg atgaaccgac aggcgcgctg gattttgaaa cgggcaagca agtattgaaa
    25801 ttattgcaaa acgctagcag acaacacggc aataccgttt tgatcattac acataattct
    25861 gctttagcgc ccatcgctga tagagtcatt cacattaatg atgctaaagt aaggtctgtt
    25921 gaattaaatg atcatccttc atccattgat gagattgtat ggtaggggga ttttatgaaa
    25981 acaagaacat atcttaaagc aggttttcgc gaaattcggc aatctaaagg acgattcatt
    26041 gcaatcatcc ttattgtcat gctaggaaca cttttatttg taggtgtcaa aactgctgga
    26101 cctgtcatgc aaaaaacgat ggatcactat gttggtacag caggactttc tgatttgcaa
    26161 atcgtttcta ctggtggctt gacacaaaaa gatattgctc aagcagaaaa gatcccagat
    26221 gcaaaagtag aaacaggaaa acagatctat tattccaata ctggcaaaaa cgaagtgatt
    26281 caaatatttt cttataacaa aaataacaag cagaacaagc tacaattaac tgatggaaaa
    26341 ctcccggaaa aaccaaacca gcttgtattg gatgaaaaag cggaagatga agggtataaa
    26401 atcggtgata tttatcagat tgattcagat gaattgaaag aaaaagagta taccatcacg
    26461 ggctttgtcc gttctccatt gttcatcaac aatttggaga gaggatacgc gaacgtcgga
    26521 aatggtagcg tcgattattt tgtctattta cctgaatccg tttttaaaac agatatttat
    26581 tctgtcttat atcttgattt tacaaacgta aagaatttga atacgtacag caaagcttac
    26641 aaagataaaa tggagcaaaa tcaagaaaag gcagaaaagt atttacaaga tcgacccgaa
    26701 gaacgcttag aagagctgaa aaaatcggcc aacgaagaat tagaaccggc acagcaaaaa
    26761 atagcggatg gcaaagcaca aacaacgcaa gcaagaacac ggttagaaga tgctaaaaaa
    26821 cagttagcgc aacagtcagc aactatccag cagcttcctg aagcgcaacg aacaatcgct
    26881 gttcaaacat tatctcaaca agaagctcaa cttgctgaac aagaaaggca attagttgag
    26941 aaagaacgtg aactggcctc tgcagaggaa gaattgaatc gaaatgcaga aaaaattgaa
    27001 aatttaacta aaccatcata cctatacaat gaacgttcag aaaatgtcgg ttttcaagaa
    27061 tttggtgatc tgtctgatcg tatcgctgcg attgccaatg tttttcctgt tttctttttc
    27121 tttattgccg cattgatcac ctttacgaca atgacacgta tggtagaaga aaaccgcagg
    27181 gaaatcggga cattaaaggc gttaggttat acgaaagttg aaatcgcaaa aaaatatgca
    27241 atctatgcaa gtctagcctc agggataggt attgttttag gaacgatatt aggaacgaat
    27301 cttttgccaa ggatcatttt tgagctgtct aacgagcggt atgatattgg gagtgcgctt
    27361 attttttatg actggccgcc gattttacaa gcagctgccg cattcttcat tgccgcattt
    27421 ggcgctgcaa tgatcgtttt attcaaagat ttaagagaac ggcctgctgc attgcttcaa
    27481 ccgaaagcac cgaagccagg gaaacgcatt tttcttgaat acatcactcc gctgtggtca
    27541 cgccttagct ttaatcaaaa aatcagttac cgaaatctct tccgatataa gtcaagaatg
    27601 cttatggcga ttatcggaat tgctggatgt gccgggctga tggttgcagg cgtgggattg
    27661 aaagattctc taagttctgt ttcagccaaa caatttggac cgatcattga ctatcaagca
    27721 atcgttacat tggattcatc cacggaagat agtcgtcaaa aagcagcgga tatgctgcag
    27781 aagcaagaaa aagtaaatga gatcctagct attcatactc aaaccgtaga attgagaaaa
    27841 aaaggacatg cttcgcaaag tctgacagtg atcgtaccaa gtaaaacagc tgatttggat
    27901 cgttttatcc atttaactga tcttgaagag aacccacgct ccttgcctaa aacaggaatc
    27961 gctattacgc aaaaagtagc agaactattt gacttatcta caggagatca tatttcgctt
    28021 tatgatgaag atcaaaaaga atggaaagtc gaagtaaaag aaatcgtgaa aaactatcta
    28081 ggtagcttta tttatatgtc ccctgaatat tatgcttcag ttactgatca atcgatgatg
    28141 gataatgcat ttttgctgcg tacagataag atgacaacaa aagaagaaga aatgctttcc
    28201 gagaaactgc ttgccacaga cgctgtgaca aatacgtctt tcatgtccaa acaaatcgaa
    28261 acgcaagaag aatcaatggc gaacttagac tcggttgtct taatctttgt cgtattatca
    28321 ggactgctgg cttttatcgt cttgtataat ctaactaata tcaatatttc cgagcgtgta
    28381 cgcgaattat ctacaataaa agtccttgga ttttttgata aagaagtcac gatgtacatt
    28441 gttcgagaaa atatcatctt tactctttta ggaatcatcg gaggatttgg tgtaggatat
    28501 atattgactg acttcatctt gcaacaagcg tctatggaga atgtcatctt tccattagtg
    28561 atcacatggt gggcttatgt cttatcagca ggattaacta tcgtatttac ggttattgtg
    28621 atgattgtta cccatttcaa attaaagcat atcgatatga ttgatgcgtt gaaatcgaat
    28681 gaataaaaac agaggttgtg aaatcagcgg tttgacctga gtattagaga aacaaacggc
    28741 aaaatagttt ttcatatttt gccgtttttt gagctaatac cgaaggacag ctgattgaac
    28801 accatgaatc agaggttgta aaaaagccgt cctgcatcga gcaaaaagaa tactcaaata
    28861 aaattagctc ctcatgtttt acatttgaga agactgattg ccgtagataa aagcttttga
    28921 acaccattta tagaagaaac aagaatattt tgctatttta aacaagagga tagacaaata
    28981 catgtctgat ctcttgtttt ttattgggca acagacctct tagaatgtat aaagataaat
    29041 tttacaaacg ctttcggttc agttataata ataaatgata acttgtatat ataagttgtg
    29101 gtaggtaata aatcagttga atggaggtaa atatccatga catttaaaga caaagtcatt
    29161 taccaaatct atcccaaatc ttacaaagac agtaatggtg atggaattgg agatttaaaa
    29221 ggaatcaaag aaaaactccc gtatctgaat gatctaggca tcgacatgat ttggctaaac
    29281 cctatttatc caagccctca aaaagacaac ggttatgata tctctgatta cacagccatc
    29341 gatcctatct ttggtacgat ggaggaattc gaacagctaa tagccgaagc aaagaaatac
    29401 cggatagaaa tcatgctcga tatggtgctg aatcacgttt cgacagaaca tgaatggttc
    29461 aaaaaagcac tagcagggga ccagcattat caggacttct tcattttaag agaagagcca
    29521 acggattggg tatcaaaatt tggtggaagt gcttggtcgc catttggaga tacggggaaa
    29581 tattatttgc acttatatga tcggacacaa gctgacttga actggcggaa ccaagcagta
    29641 agagaggaac tattcaaagt tgttcgtttt tggatggaaa aaggggtaaa aggattccgt
    29701 ttcgatgtta tcaatgttat cggtaaagac gaacaattga aaaataataa agaaaatgat
    29761 ggcaaacctg aatatacgga caaaccaatc acccatacgt atctaaaaga attgaaccaa
    29821 gcaacgtttg ggacggattc cactatcatc acagtcggtg aaatgagctc tacgacgatc
    29881 gaaaattgta tcttgtatac agaacctagt agagaagaat tatcaatggt ctttaatttc
    29941 catcatttga aagtcgatta tgaaaatggg aataaatggt ctactcctcc atttgatttt
    30001 gcagaattaa agcgactatt tcatacatgg ggagaaaaga tgagtgaggg taacggctgg
    30061 aatgctctat tttttaataa tcatgatcag ccacgtgcgc tcaatcgatt tgtagctatc
    30121 gatcagtatc gaaaacaagg agcagagatg ctagcaacca tgatccattt gaaccgtggg
    30181 acgccctata tttatatggg agaagaaatc ggcatgatcg acccagacta ttcatcgatt
    30241 tccgactata ttgacgtaga aagtttgaat gcttataagt tactccttga acaaggattc
    30301 acagaaaaag aagcgtttcg tcggatcaaa gcaaaatctc gcgataactc tcgaacaccc
    30361 atgcaatggt cagacacaga acaagcagga tttactacag ggaaaccttg gttaaaagtt
    30421 ggaggcaagc tagaagagat taatgtagaa aaagaactat cttctgaaga ttctattttg
    30481 tcctattaca agaaattgat tcggctgaga aaaacatatc caatcgttgc acaaggagat
    30541 tatcatgctt atgaagcaaa tcatccggaa gtttatggct ttcttcgcca atttgaagga
    30601 cagcagctgc ttgtattaac aaatttctat gataaagata caaggatcgc catcccagaa
    30661 ggattcacag aagccaatat tcttatcgat aattattccg atactgttct tcatcaaagt
    30721 ctggagctaa aaccatacca ggcaattgct ttgctgaaaa attaaaacaa aaacacaaaa
    30781 cgtcgttcct acgagctatt ctcgtaagat cgacgttttg tgttttattt aaaataatct
    30841 tcttcagaaa atgaagagtt ttcatactct cctttatcgc cataaaattt attgtagcgt
    30901 tgtttgaatt tacggaaaat aaaagtaacg agtaatttga ctaacgtata gataggaata
    30961 ccaaaaataa cgcccacaat tcccattaga tcacccatga caagtaaaac gattaagatc
    31021 gtaatcggat ggatctgtaa acgatcaccc attactcgag gaatcaccaa gtcaccgtgg
    31081 agaagttgta cggcaaacca gacaatgaca aatttgacag ccataaaagt agagtcttgg
    31141 aatgcaatga tcaaacctgg aaagaaggcg ataaacggcc caatatatgg aatgatacat
    31201 aacaacccag cagaaatagc taaaacacta gcaaattcta atccgatcag taagaaacaa
    31261 acccagtaga ctgctccaag gataaatgaa gcaatgattt gcccctttag aaatgaaccc
    31321 aattgttgat tagcaatctt tgtcaaattt ttaaaatctg tacgaaatgc tggtgggaca
    31381 atcgctaaaa ctgactgata aaatttttga ggatctctca aaaggaaaaa agcgacaatt
    31441 ggaccagtaa ataaagtaat aaaaatagct gtaacagttg aaaaaatgtt gctgatccgt
    31501 tgtgcgccac tttcccaata atttccaata aatccagcta tatcagacgt cacgtcgtca
    31561 atagaagcga aaaattgatt aaatgaatcg gcaaaaggtg ttttctccaa aaattgtcgg
    31621 agactctctt gaaaatcttg aaacaaatct ggcaaactac tgatcaatgt ctttgcctgt
    31681 tcttcaagaa aaggaataag ccatatgctt ccaagcccca ataaaaataa aataatcaca
    31741 taaacgataa tgactgacaa gttcctcgaa aggtgcttgc tcaaacgttt gatgactggt
    31801 tccaaaagat aatacaagat cagtgcaaac aataatgggg gcaagatagt agcgataatg
    31861 attccgacag gcttgaatat aaacgagatc tgatggaaaa aataaatgat cagcgcaagt
    31921 aagataacta ttcctaaaac atagtaagag gtttttcctc ctaaaaagcg gatcaaacga
    31981 ttcgttgtat ctgtttcttt tttcactctt tttcctcctt tatctataac ttcattatag
    32041 acaaaagcgg gtataaaagg aaaatgatag aggttgattg caaaaatatc gtcaatcatt
    32101 catataaact ttctcatcaa ttaaaataag aaaacgcttg acagttcttt taaccatatg
    32161 ataaattaaa tcctgttgct aaaacgtaac tattacggtt tgcgaaaagc gaatcggaac
    32221 attcaaatga aagaagatga gaagaggagt gtcataaatg aaacgattga taggtatatt
    32281 ggccatgctg gttttagcag gtatgttaac tgcttgcggg gcaagtggga aagccgagga
    32341 cagtaaagaa aaattgagcg tgatgacgac cttttatccc atgtatgatt ttacaaaagc
    32401 aatcgtcggg gatgaaggag aagtagagtt attgattcca gcagggactg attcccacga
    32461 ttatgaacct tctgctaaag acatggcaaa gatccaagat acagatatct ttgtttataa
    32521 tgatgaaaac atggaaacat gggtccccgc aatccaaaaa acattacaag aaggcaatgt
    32581 ccatacgata aaagcgacag aaggtatgct tttacttccg ggaagtgaag aaggacatga
    32641 tcatgaccat gaacacgggg aagaaggcca tacacatgag ctagacccac atgtatggct
    32701 agctccaagt ttagcaatca agcaggtagc aaacatacgt gatcagctga ttgaagctta
    32761 cccagaaaaa caggaagtat ggacaaaaaa tgcagcagcc tataccgaga aacttcaagc
    32821 actccatcag ctttatcaag aaacctttaa gcaagcaaaa caaagatctt ttgtcacgca
    32881 acatgctgca ttcaactatc tagcgttaga atatggattg aatcaagtat cgattgctgg
    32941 tctttcttct agtgaagaac cgtctgcggc gagaattgct gaactgaaac actttgttaa
    33001 agaacatgga atcaactata tttattttga agaaaacgcc aaagatagta ttgccagaac
    33061 gttagcaaac gaagcaggtg tatctttaga agtattgaat cctttggaag gattgacgaa
    33121 tgaacaaata gaaaatggag aaaactatct ttctatcatg gaagcaaacc tcgaagcgtt
    33181 gaaaaaaacg acagagacag aaaacccgct agaagatact ttgaccccca aaaaagaaaa
    33241 gagcgtctac aacggttatt ttgaagacga ggatgtacaa gatcgctctc tttcagattg
    33301 ggcaggaact tggcaaacag tagatacgta tgtagaagat gggacttttg atcctgtttt
    33361 tgaatataaa gaaaaattga atcaggataa gacagcacaa gaatataaag aatattatac
    33421 gaagggctat caaacagata ttgcttcaat caagatcagt gagaaagaga tggcatttac
    33481 gtttaagaat ggaacaacga aacgttcaaa ttatcgttat gtagggaaag aaatcttgac
    33541 ctacgaggca ggaaaccgtg gcgtaaggta tctattcgaa gcagaagata aggaaagtgg
    33601 cgcttttaga tatattcaat tcagtgatca cacgattgca ccggctgact ccgaacattt
    33661 tcatttatat gcaggaaatg aaagtcaaga agtattgctg aaagaaatgg agaattggcc
    33721 aacattttat ccagatacaa tggatggacg tgaaattgca caggaaatgc ttttgcactg
    33781 ataggcaaaa aagaaccgaa gggtttggat cggctggtca tttagtcggt ccaaactatt
    33841 cggttctttt tatgaaatca atttctttaa tccacggata gaggctggga tggtctcgcc
    33901 gtttgccaac gtgacttctc tggttttttt attaatcgac cgcacatttt gtgtattgat
    33961 aacataggat ttatgacaac ggataaaatt ccgatgcatt ttctcgattt ctttgatctt
    34021 ctcatagaat tgtaattggg catgagtcaa atggacttcc agtttatgtg gcgctcctag
    34081 cgaagtagaa aaatattgga tgtctttgat cggtaaaaat ttgatctcat cattaaactc
    34141 tattttgaag atatcttttt ggatttggga catttgaaaa tcttcgaaaa ttttgctcag
    34201 gatatgctcg acattttctt ttaaatcata aagatcttct ttgacgatat aatcagtcgg
    34261 ttcgatgttg ctttttaaga ttttcatcga catttccgta tgggaagtca catagattat
    34321 ttttcctaaa ggatctaatt cacggatcaa tttaccgatc gctacaccat ccatttcact
    34381 gtcttctagt tcgatatcta aaaagtaaat accaaaagaa gaagttctga tcttggctgc
    34441 attgagaagt tcttccggat cagaggtaga aatatggaca taactgtcta agttttcaat
    34501 catgattcta tttttgatca ccgttgataa aacttctaat tgttttggat cgtcttcaca
    34561 aatataaatg ggaatcatat cattactcct ttattttatt tg
//
LOCUS       DAZKKW010000024        34579 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.024, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000024 DAZKKW010000000
VERSION     DAZKKW010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34579
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.024"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(243..2135)
                     /locus_tag="ACG8PO_001108"
     CDS             complement(243..2135)
                     /locus_tag="ACG8PO_001108"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0206451.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(2132..3859)
                     /locus_tag="ACG8PO_001109"
     CDS             complement(2132..3859)
                     /locus_tag="ACG8PO_001109"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0206452.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(3875..4524)
                     /locus_tag="ACG8PO_001110"
                     /pseudo
     CDS             complement(3875..4524)
                     /locus_tag="ACG8PO_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
     gene            4728..5024
                     /locus_tag="ACG8PO_001111"
     CDS             4728..5024
                     /locus_tag="ACG8PO_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFQ0206453.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            5196..5816
                     /locus_tag="ACG8PO_001112"
     CDS             5196..5816
                     /locus_tag="ACG8PO_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFQ0206454.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(5908..7074)
                     /locus_tag="ACG8PO_001113"
     CDS             complement(5908..7074)
                     /locus_tag="ACG8PO_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFQ0206455.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(7086..7439)
                     /locus_tag="ACG8PO_001114"
     CDS             complement(7086..7439)
                     /locus_tag="ACG8PO_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFQ0206456.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLETVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            7567..8589
                     /locus_tag="ACG8PO_001115"
     CDS             7567..8589
                     /locus_tag="ACG8PO_001115"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HFQ0206457.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            8608..8769
                     /locus_tag="ACG8PO_001116"
     CDS             8608..8769
                     /locus_tag="ACG8PO_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0206458.1"
                     /translation="MNQQDVLDRLREELNIPFFDGKIEDKEYSEEEYQKLKADLQNYF
                     EQYVQNVEN"
     gene            complement(9028..9462)
                     /locus_tag="ACG8PO_001117"
     CDS             complement(9028..9462)
                     /locus_tag="ACG8PO_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0206459.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLKKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(9572..11431)
                     /locus_tag="ACG8PO_001118"
     CDS             complement(9572..11431)
                     /locus_tag="ACG8PO_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFQ0206460.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            11432..11572
                     /locus_tag="ACG8PO_001119"
     CDS             11432..11572
                     /locus_tag="ACG8PO_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoglycerate dehydrogenase"
                     /protein_id="HFQ0206461.1"
                     /translation="MLAPSFLSIKECCFSYFSCTKFESKERKEELMKSKYSLSLWPFW
                     FL"
     gene            complement(11549..12709)
                     /locus_tag="ACG8PO_001120"
     CDS             complement(11549..12709)
                     /locus_tag="ACG8PO_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFQ0206462.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(13016..14440)
                     /gene="araA"
                     /locus_tag="ACG8PO_001121"
     CDS             complement(13016..14440)
                     /gene="araA"
                     /locus_tag="ACG8PO_001121"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HFQ0206463.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(14456..15154)
                     /locus_tag="ACG8PO_001122"
     CDS             complement(14456..15154)
                     /locus_tag="ACG8PO_001122"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFQ0206464.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(15111..16772)
                     /locus_tag="ACG8PO_001123"
     CDS             complement(15111..16772)
                     /locus_tag="ACG8PO_001123"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0005998 - xylulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HFQ0206465.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKR
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            16990..18390
                     /locus_tag="ACG8PO_001124"
     CDS             16990..18390
                     /locus_tag="ACG8PO_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051119 - sugar transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HFQ0206466.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKVLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIIGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(18642..19721)
                     /locus_tag="ACG8PO_001125"
     CDS             complement(18642..19721)
                     /locus_tag="ACG8PO_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0206467.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(19807..20766)
                     /locus_tag="ACG8PO_001126"
     CDS             complement(19807..20766)
                     /locus_tag="ACG8PO_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HFQ0206468.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSKPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(20778..22289)
                     /locus_tag="ACG8PO_001127"
     CDS             complement(20778..22289)
                     /locus_tag="ACG8PO_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HFQ0206469.1"
                     /translation="MKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHETADG
                     DGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIEPNSF
                     GLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQKKPF
                     GVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPRLPSY
                     PEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFDRQIE
                     EIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMEDTLLF
                     GSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAKGQVL
                     KQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLLDQGE
                     HYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(22311..22874)
                     /locus_tag="ACG8PO_001128"
     CDS             complement(22311..22874)
                     /locus_tag="ACG8PO_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFQ0206470.1"
                     /translation="MKIVETKFYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(22880..23743)
                     /locus_tag="ACG8PO_001129"
     CDS             complement(22880..23743)
                     /locus_tag="ACG8PO_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0206471.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(23757..24653)
                     /locus_tag="ACG8PO_001130"
     CDS             complement(23757..24653)
                     /locus_tag="ACG8PO_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0206472.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(24828..26117)
                     /locus_tag="ACG8PO_001131"
     CDS             complement(24828..26117)
                     /locus_tag="ACG8PO_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0206473.1"
                     /translation="MKEEQKMKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTL
                     TFWNGFTASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFI
                     ALNYPDFAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMY
                     MYWNNDLFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLA
                     NGGKLVNDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIE
                     INGPWLNNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFL
                     KYWNTTEIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQIS
                     AINNDIINPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(26089..27606)
                     /locus_tag="ACG8PO_001132"
     CDS             complement(26089..27606)
                     /locus_tag="ACG8PO_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HFQ0206474.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            27804..28694
                     /locus_tag="ACG8PO_001133"
     CDS             27804..28694
                     /locus_tag="ACG8PO_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
                     /protein_id="HFQ0206475.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGNFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(28767..29591)
                     /gene="nadE"
                     /locus_tag="ACG8PO_001134"
     CDS             complement(28767..29591)
                     /gene="nadE"
                     /locus_tag="ACG8PO_001134"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HFQ0206476.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(29603..31075)
                     /locus_tag="ACG8PO_001135"
     CDS             complement(29603..31075)
                     /locus_tag="ACG8PO_001135"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFQ0206477.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(31712..32074)
                     /locus_tag="ACG8PO_001136"
     CDS             complement(31712..32074)
                     /locus_tag="ACG8PO_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HFQ0206478.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            32297..33049
                     /locus_tag="ACG8PO_001137"
     CDS             32297..33049
                     /locus_tag="ACG8PO_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFQ0206479.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWLSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(33189..34112)
                     /locus_tag="ACG8PO_001138"
     CDS             complement(33189..34112)
                     /locus_tag="ACG8PO_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0206480.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYQKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(34127..34399)
                     /locus_tag="ACG8PO_001139"
     CDS             complement(34127..34399)
                     /locus_tag="ACG8PO_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0206481.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
BASE COUNT        10205 a         7199 c         5954 g        11221 t
ORIGIN      
        1 ttcgtttggt tgggcttatt accgcagatg caagcttttg aacaccgttt aaggaagagg
       61 ttgtaaaatc agcggtttga cctgagtatt agagatacaa aacggaaaaa tagcttctca
      121 tattttgccg ttttttgagc taataccgaa ggacagctga ttgaacaccg tttattcgga
      181 aaaagggact gtgacacgac ttttgtcaca gcccctaaaa aaaataataa atttgtacta
      241 ttctatgaag cttcttcttc agaaaactga ctgttataaa gatctgcata gaatccgtct
      301 ttttccatca atgtatcgtg gtttcctgtt tctacgatcg aaccatgatt catcacaata
      361 atgttgtctg catcgcgtat tgtagacaag cggtgagcaa caacgaaact tgtccggttt
      421 tccaataggc ggttcatcgc tttttgaatc agtatttctg ttcgtgtatc tacactagat
      481 gttgcttcat caaggatcag gacatctgga tttgctaaga atgcccgcgc aattgtgatt
      541 agctgacgct gcccttgcga aatattgctt gcatcctcgt ttaatacagt gttatatcct
      601 tctggcaatt tacgaacaaa atcgtctacg tgagcagctt tagcagcctc aatgatttgt
      661 tcgtctgttg catcgtcgtt cccatatttg atattgtcat agatacttcc tgtaaacagc
      721 caagtatctt gcagtaccat tgaaaactgt gcgcgcaatt catcacgtga aagatcacgg
      781 gtatctacac catcgtaacg aatactacct ccagaaacat cgtagaaccg ttcaagaagg
      841 ttgatcagtg tcgtcttccc tgcaccagtc ggacctacaa tagcgaccat ctcaccaggt
      901 ttgacatcca agttaaaatc agtcattagc aagttatctg gtgtgtagcc gaattgaaca
      961 ttttcaaaac gaactttgta tggtgtatct ttttcttctg gaaggccaga tttttcattg
     1021 gacatctctt cttcatccaa cacttcaaat acccgttcgg ctgaagcaac tgttgattgg
     1081 atagtgttca tcaaacttgc aagttgagtg attggttgag agaactgtgt tgtatattgc
     1141 aagaacgctt gaacatcccc taatgtcatg ttcccatttg caacttttac tccgcctagt
     1201 acagcgacga acacatagcc tagattttta acgaaattca tcaacggcat gatgattgcc
     1261 gaaataaatt gagctttcca accagcggca tacagattct gattttcttt ttcaaatact
     1321 tcctggtctt tttcagtatg attgaatgtt ttaacaatgg tatgaccacc atatgtttct
     1381 tctacttggt tattcaataa acctaaactt ttttgctgat ctgcgaaaaa cacttgtgat
     1441 ttcggcgcta cgatcatcac gatgatcaag cttaaaggga tcatcaataa cgcgactaac
     1501 gtcaattgcc agctgattga aagcatcatc acgatgaccc caatcaatgt gactgcacta
     1561 gttacaaatt gagtcaaatt ttgctgcagc gtgcttgcga tgttatccat atcattgatt
     1621 gctcgagaca tgatatcccc atttgtatgt gtatcataat aggaaatcgg tacacgattc
     1681 attttggctt ccaaatcatg acgtaattca taaactgttc gttgggaaac acgtgtcata
     1741 ataaattgct gtaaaaagtt gaatacagca gaaatcaaat acatcacgat tactgtcgca
     1801 atgatctgcg cgattttatc aaagtctatc ggaaattttg ttacttgttg gccttgttgc
     1861 atctgttgag cgccagccat tacacctttg aatatttctg tggttgcttt ccccaagacc
     1921 tttggtgttt ggatttggaa gatcacggct gctattgcta gtacaaatac agcgatgatc
     1981 gcaaccatac gtttagacat atagccaaac aatcggcgta ccgttcccca gaaattttta
     2041 ggcttttcac cttttacacc gagatttctg ccaggtccac catgtcctgg accgcctgct
     2101 ggtccatgag agggacgtgt gtttgtttta ctcatgctaa gtcctcctct cttagttgtg
     2161 aatgaacgat ttcttgataa gtttcgtttg ttgctaatag ttcttcatgt gttcctttgc
     2221 caaccagttt tccttcatct agcacaagga tcatatctgc atccattact gtacttactc
     2281 gttgagcaac taaaacggtg atactttctt tcatattcgt tttcaatgct tggcgaagat
     2341 tggcatccgt tttgaaatcc aacgctgaga aggaatcatc aaacacatag atatctgctg
     2401 ttttgactaa tgcccgtgca attgccagac gctgtctttg tccaccagag aagtttcctc
     2461 ctccttgttc cacatggcta tctaagccat cttccatctc tgaaacaaaa tctttagctt
     2521 gtgcgatttc taatgctttc caaatcattt catctgttgc atccggctta ccatactgca
     2581 tattgtctcg gatcgttcca gagaacagaa ctgctttttg aggaacaaag cccattaatt
     2641 ctcgcaaatt atattgagtc atgtcactaa tgtccgttcc atttatttgg atcgaaccag
     2701 attcgatgtc atataatcga gggatcaaat tgactaaggt cgttttacct gaaccagtcc
     2761 cccctatgat tgcgacgatc tcgcccgact tggcttggaa atcaatatct tccaatgcaa
     2821 gtttttctgc tccatgatat cgataattga catgattgaa ggccaatgtt gcgttttttc
     2881 cagcaaaaga aactgtttta ggattttcag ggtcatggat accgattttt tcatccaaca
     2941 cttcattaat acggtcagct gaagcttgtg cacgaggcac catgataaaa atagcggaaa
     3001 gcatcataaa gctgatcaag atctgcatcg cataagtcat aaatgcaatc agattaccga
     3061 cttccaaagt catatctgct atgtagtgtc caccaaacca agtgattgct acgtttgtac
     3121 cgctcatgat caatgtcatc attggcatca tcaatgcgac gatcgtatta actttgattg
     3181 ccgtatttgt ataatcttta ttcgctaaat caaaacgatt ttcttcaaag cgtgtcttat
     3241 cgaatgctcg aatcactctg acccctgtca atccttctcg aaataccagg tttaaacggt
     3301 cagttttctt ttgcatactt ttgaataatg gaacagcaaa gtacatgatt ccgccgatca
     3361 aaataatcat tactgggatg acaactaaga agatttttgt caactgatga tctttttgat
     3421 aagcaagtat gctcgcgcca atcaacgtga ttggtgcatt gatcatcata cgtaaaaaca
     3481 tttgtgtaac catttggatt tgagtcacat cattggtcgt tcttgtgatc aaagaggccg
     3541 ttccatattt atcaaacgca tcttttgtca gactctctga ttttttatag acatccgtac
     3601 gcaattgctt acccagtttc tgtgattcac gagtagcaaa aaatgtattt gcgatagcag
     3661 ctaagatact gatcaaagaa aagccgatca tcacaaaacc tgtatgccaa atataatcga
     3721 catctccttg cgctacccct ttatcaataa tattggaggt aagtgtcggc aaatataaat
     3781 ctgccagtac ttggatcacc atgaaaactg cagctgcaat tgctgaagtc aaggagatcc
     3841 gttttacgat ttttagcatc tagttccctt ctttctattt atatactccg tttttcagcc
     3901 aagaaagttt caaattgaat tcactgattg cttgctctac tgagtaatct tcagatgttg
     3961 aaataagcga tacgaatgcg caattgttgt aaatacagta ttcatcacta attggatcag
     4021 taattccaat tcatgatcat tttttacttt cagcttcgtc cgatcgatca attcaagcaa
     4081 ctcttttgca tcttgtttgt gttctttatg tggatcgtga ggcgtttttt ggcgactcgt
     4141 atgaggagcg acttttcttg aggcacggta atccatgttc ataaacaagt tacgataaaa
     4201 agaagcattc tcgccttcca aaatctcata aatcatttta gaaaaatagg attcgaatcc
     4261 tttgaataga tcaccatcgg attctttcat tagttgtaac atatctttag agctgtcacg
     4321 tctcattgtc tcaaagtaat aaaaataaag atcctcttta tcttcaaaat actgatagaa
     4381 actgcctcta gggatatccg ctaatttgac gatattagca atcgaagctt cttttaaggg
     4441 tactctagaa aactcgattc tcgcagcctc gattagtctt ttttgttttt ctttaggcaa
     4501 atggaaaaat gtctgtgtcg gcatatggat cctcctttct ttcaagatga cacattgtca
     4561 tatgacgctg tgtcattata acacctccta cctgttttgc aagacatttg catagttttt
     4621 ttcattatca tccgatacat taaaaaagtg ataagaggaa tccttccatt tgaattaatg
     4681 agagttgccc attaaaatag ccacatacta aacaaaggat gagataaatg gcccaatcaa
     4741 ttagtgtcca agacttcaat aaattgcctc tcgatgctga cagcatcgtt ttagatacaa
     4801 gaaatacctt agattacaca aaaaaccact taagccactc gctttctatc cccgctgcta
     4861 tcttgccaag acaattaaaa aatctagatc caaagaaact ttattatatt ctaagctata
     4921 caggcaggcg atcagaagca ttagccgatc aacttactgc taatggtttt cgtgctgttt
     4981 atatcattgg tgggatggaa gctcttcagt cgagtgttgc ttgaatattg cttgttagat
     5041 tcaaaagagg ctgtgacaaa aaatcgtgtc acagcctctt ttataccctg tcttttttta
     5101 gttatttatt cttcaacctt atcaatatga cttgatagcc ttttcttttt ctcatagtta
     5161 taacaatcat agcaataaaa ggagaaggta agcatatggc atctgtttta gtagtcaaag
     5221 gtcatcccct aactgcagaa gaatcgcgca cagttaaggc attgacgtct tttttaacaa
     5281 gttataaaga aaatcatcca gatgatgaag tgactgtttt ggaactttat cgcgatgata
     5341 ttccggaaat cgatgaagaa ttattatcag gttgggaagc attacgtgct ggtgcagaat
     5401 tcacttcctt atcagaaagt caacaacaaa aaattgctcg tttcaatgaa ttgactgatc
     5461 agttccttgc tgctgataaa atcgttatcg caaacgcctt atggaatcta aatatcccaa
     5521 caaaattaaa agcttggttc gataccgtca atgtagctgg aaaaacgttc cgctatacag
     5581 aaaacgggcc agaaggatta gtgacaggta aaaaagcact gcacatccaa tctaatggcg
     5641 gtgtatacaa cggacaagac tttgcctctc aatatgtaaa aggtatttta aactttgtag
     5701 gcatcgatca agtagatcag ctattcatcg aaggaatcga ttatgaccct gaccgtgcag
     5761 atgagttgat gcaaaatgca ttagataaag ctgctgcttt aggtaaatca ttttaaaaat
     5821 tgagataaat gcatacaaaa gagacagagg aaaaaatctt caaagcagca tttgtacctt
     5881 ggatattttc ccatctgtct ctttttgtta ttctttctgc tttttcaacg gattttcttc
     5941 ataaagagaa gagattgttg tgtaccattc aaaaacatga gtaatcacta ctttagctgc
     6001 tgcataaaca ggaatcccca agatcacacc gatgattccg aacaatttcc cagaagtcag
     6061 cagaactaat aaaatcgtta ctggatggat tgctaactga ttccctagta ctaatggaga
     6121 gatcaagcgt ccttctattg tctgctctac tgcaaaaacg atcagtactt tgatgatcat
     6181 gaatggacct gctacaatgg ctaaaaagat agccggaatc atcgctaaaa atgatcctaa
     6241 gtaagggatc aggttcaaaa aacccgcaat aatccccaac gtgacagcat actctaaacc
     6301 aatgaccgca aaaccaatca taaacatgat tgccacagca aaagccacag ttagttgtcc
     6361 tcttatataa gaagaaactt gactattcat ttcactcagc acattcaacg tcggtttttg
     6421 catgcgtgtc ggtaaaaatt tgacaaaata aggtgctaat tgtttaccgt ctttcaatag
     6481 ataaaataag ataaacggca tcgtcagcag agccactaaa atagtcgcta ccgcgccgac
     6541 aaaacttcca agactctgaa ctgtactctt agatatatct tggatcatat cgcctgccga
     6601 attcatgaac ttatcacctg ctgtttccaa ctgttcgcga aattggttga acagcgggtc
     6661 ccgcaaaatc tcggttaatt tattatcaat cgtatctaca tattgaggga agttactgat
     6721 gaagctcact gtttgttcct gaatcttagg gatgatcact acgctccccc atgcgattag
     6781 tgcaacaacc aaaacaaaca gcccaatgat actgtaaagc cttggtactt ttcgcttttc
     6841 aaagtaatca actaccggat tcatcaagta atacaagatt cctgctaaaa tgataggcag
     6901 acctacgatt gctaaaaatt gccagattgg tgcaaacaaa tatgaaactt ttgtaaataa
     6961 aaagacgatc agcaatatta acaatacaac aagtaaggct gtgactactt gattatttaa
     7021 aaaccagcgc caaaaccacg ataccggtct gttcttctta ttttttcctt ccataattcc
     7081 tcctgttagt tataaaaaat ttggcttccc aaaccaatag caggcagtct atgcggatga
     7141 agatagattc catttgcaat ttgtccttct tctggacaag catcaaaaat caatgatacg
     7201 tgagctactg tctctaaatt cccattggcc atcggaccga cataatcgat cgtatatacc
     7261 tgatcatcaa aagcaattgt tccgcctgct ttcaaagaaa aaggttcgtc ttttgatact
     7321 tcttgaatca ccgaatattt tcgtaaggca gctgttgctg ttttatcgaa caaaatcagc
     7381 atgggttctt ccttgtttat tgcttctgtc ccaatttctg taatctttgc ctgaatcatt
     7441 gcactctctc ctttaattgc tttcaaaaac agtataacac attctcattc tttagccgtg
     7501 tggaataatt atgtgataga ataaaaacga aaactatttc attacaatta ggaggaatca
     7561 gctcatatga tcgaaaagat aattttagga acctatacgc gtcgcgaaag taaaggaatc
     7621 tatacgatcg accttgatac agaaaaagaa gaattagcca acctttcatt agctgttgaa
     7681 gaaaatagcc ctacctatgt agcaaaaagt aaagctggca atctttacac agttactagt
     7741 gtggatggat taggtggtgc cggtgcgtac gataaggatt ttcatttttt gaatgcggta
     7801 acagagtctg gcgctccttt gtgttatgta gcggttgatg aagcacgtca attactatac
     7861 ggggcgaact atcataaagg tgaagtaaat gtctaccata tattaggaga tggcgaactg
     7921 aaagcagcag attcaatatt ccatcaagaa gcgacaggtc ctcataaaaa ccaagatcat
     7981 gcgcatgtcc actatacgga tctgactcct gaccaacgat tagtcgtatg tgatcttggt
     8041 acagatcgtg tgtatactta tgatgtgtcc gaaaacggca aattgaatct tgtaactacc
     8101 ttcactgcag agccaggaac aggccctcgc catcttgtct ttcatccaaa tggacaattc
     8161 gcttatttat ttggtgaatt agacagcaca gtccgcgtgc tttcttataa taaagaaaca
     8221 ggtgcttttg aagaattaca gaaaatatcg accttgccag aagaattcga cggtgaaaat
     8281 ggaggggcag ctatccgcat ttcaaatgat ggcaagttcc tttatgcttc taaccgagga
     8341 cataactcga ttgctgtatt cgcagttttt gaagatggtt cccacattca aaacaagcaa
     8401 atcatctcaa cagaagggga ttttccccgc gatttcgcac tagatccaag caataatttt
     8461 gtcgtgtgtg ccaatcagaa caccgataat ctgacattat atcgccgtca tcctgaaaca
     8521 ggattactgg aattactgaa aaaagatgtc tatgtaccag aatgtgtctg tgtttatttt
     8581 gatcgttagt aagaaaagga gaatgaaatg aatcagcaag acgtattaga cagactacgg
     8641 gaagaactaa atattccttt ctttgatggg aaaatcgaag ataaagagta tagcgaagag
     8701 gaatatcaga aactcaaagc cgatttacag aactactttg aacaatatgt acagaatgta
     8761 gaaaactaat cagctttcca tactatctga aaagaacatc aggacaagac ttttccgtct
     8821 atcctgatgt tcttttctac aaataaaaaa ccgccgagat ggttagactc gacggaaaag
     8881 gagtatttac tcgattttga gtaagtacaa ggttgttgtt ggttataggt atgtcattat
     8941 cataaaggat tttgatcact ctatcataaa ggtaatatta catttatatt agataactct
     9001 agtttttatt gttatcaaag atttctttta ttttttcaac aacaaattag tcattttttg
     9061 gatggcagct gtcaattggt ctgtcgcttg gtcttgtttt gcttcagatg tattgaatag
     9121 atcttcatct acccctgatt caagatcgat actttggatc atttcttcac agcctgctac
     9181 atatgaaaga taagcttttt caaaattttt atgaatgccc attgcttttg ctggtggacg
     9241 caaagtactg actttcttaa gcattgcccg atattttttt gttccttctt caaataacag
     9301 gatgatctct tcaagagtcg atttgtccag ttcacttgtt tttccttcat caattgcgtt
     9361 tctgatcact tcatatttcg gatgcattcc ttctccgatt gcttctgttt ccttgacgat
     9421 ttgatcgatg gactgcacat aatagccaat attcggtaac atgttcgttt tcctactttc
     9481 tccattgttg ttattattta ttctacttgt ttttatcctc aaatgacaag tacttgaaaa
     9541 aatttaatgg tttctataca gctcgccatt cttattcgtg ctcaaagaaa ccatctgcta
     9601 aactatattg tttccctgcg acaatgtcat attggatcaa ttgatattcg tcgtaaatct
     9661 cttgttgcga gcgattgaat tgcgcttctt tataccactg tccgttttga tagtacagtt
     9721 ctcgttcaaa cgccggaagt tcctgttcta atgataaaag cagctgataa aagccatttg
     9781 ttgtctgatt cgtctgttcc attagattcg ctgcaaaata aaacggactg gtgattgcat
     9841 cgtgagtcgt ttgcttttct aattctcctt tactgtcgaa cataagaaat tgtgtttcat
     9901 gcaatgtttg tttgtcattt tttgcttgga tcgtatctga atagatccct ggaagatgat
     9961 ccccccaaaa aacaactaaa gtccttctag aaagattctt caattcttcc gtgaactgct
    10021 tcagtgccgt gctgctatag gaaatatctt gcagataatt ctctaagtca agtgtcccac
    10081 ttccatctga agtttttgca ggatagtcta gattatcata ctttccatcg tagggcatat
    10141 gtgtctgcat cgtgactaaa tgaatgaact gaggtgtttt ttcttccttg agaagtgtca
    10201 ttacttcttt gtaagctgac tcatcagaaa tatatggatt attctctatc gtatccgtat
    10261 aggtcatcgt acgctcgctg atgaactggt caaatcctaa tgtctgataa acatcctctc
    10321 ttttatacat cgaagtattg tatggatgga tcgctgtcgt ctggtatgat tgtgcgttta
    10381 acgctgaaac gatagaagga agctgatcca tttttggtac aagcatcgtg taaggggtgg
    10441 tgagttgcgc attaaataac gccatagaaa aaccagtcaa tgcttcaaat tcgatatttg
    10501 ctgttccacc gccatagttt tgagaaagca tattgccact atatgtttga tcagctactt
    10561 cataataatc tgccaatggc tcaccagata cttctacgcc gtttaatcga ctaggatctg
    10621 agaaactttc actcatcacg aacacgatgt tcggcgactc ttcctctaca gttttatttt
    10681 tttcatcggc tagcttctgg tatttttcag tgatttcttt gatcttttct ttcgaatatc
    10741 cttcaggttc atccattggt tctaccttca gattgtacaa aaacccaccg ataaaaccag
    10801 tgttatagta gttcattttc tggctgtaag gtatccatag tgccgtttta ttatatgctt
    10861 ttcgcaacaa attatcggga ttgttgaaat tactgatata ccctagcagt ccaattgcca
    10921 caagcagtgt cgttaatcgg atcaattgga atcgccttcc ttttttcagg cttcgaaaca
    10981 tgtaccagca aaacaatcct atcacggttc ctgcggcagc taagatcact gtaaacagca
    11041 tagtgccggt catatctttc agcaatccga tttcagtgat catcttcaaa tcgtctggat
    11101 aaatcggttc ttgccgataa ctcattttca tataatttgc aaaacctaat acaccgatgg
    11161 ctacgacata cgtaagggaa cctaaaggga cagatcctgc tagtgcaatc agaaaaataa
    11221 gaataatcag caagacgaga caggctaaga agaatttttc cgtatgccaa gaaaaagcaa
    11281 atttcaacgc aaggtctacg gatagatcat tttgacacca ctgcaaaaat aaattactaa
    11341 aaacaacaag aaaaaagata ctgatgattg ctgctgttcc ttttaaaatc tttttctgtt
    11401 tggtcgaaag tctgcttata tatcttttca aatgcttgct ccttcatttc tatcaataaa
    11461 agaatgttgc ttctcttatt ttagttgtac gaaattcgaa agtaaagaac ggaaagaaga
    11521 attaatgaaa tccaaatatt ctctttcttt atggcctttc tggtttcttt aataagcgga
    11581 cctttcgtac tccagaaatt ttttctagct gttgtttcac ttgattgagc tgttcaaaat
    11641 cagtactgtc tatgtcaatc aatatgtaga catgctcatt cttatggtta cgcgatatat
    11701 ctgcgatcgc tagctcgttc tcattcagca gagaaaatat ctctgctaat atattggttt
    11761 tcttttgata aaatatagtg aatcgaaacg gcgcttggaa aaacattcgt gcggatggat
    11821 aattgaccgc ttctctaacc gtaccgaaca aaagataatc ttttaaggct tcaactgcca
    11881 aacgtccgct gtctgccaaa gcttcctgag tcgatccccc tagatgaggc aacataagta
    11941 tacggtcatt ttctaagaac tcttcttctg gaaaatcagt caagtattta gccagatgtt
    12001 gtttttccaa tgcttgcata acagcatatt tatcaacaat ttcacttctt cctacattga
    12061 taagtacagc atcttttttc attttttcta tattcgcctg atcgatcaat ccttttgtat
    12121 cttctgtcaa cggcagcatg actacgatgt aatccgaggt agaaagaact gtttctaaat
    12181 ctgcttgccg aacataatcc aactgggcat cgcggataga ataacccaag acatccatcc
    12241 ctaagctata gcaacttagc gctacttttg tgcctatcgc tccaagacca agaagaccga
    12301 tggttttgcc ttgcaattct cgtcccacat aagcacttct tttgttttct gcctgttcta
    12361 aaatattagg acctgttagt gtttgtacca tacgacttgc ttcgattatc ggacgggaag
    12421 ataagagcaa acagcacaaa acaagctctt ttactgcatt agcatttaca cctggtgtat
    12481 tcatgacgat cgtcccgttt tcagaagctt tttcaacgtt gatcgtgttc accccaacac
    12541 cagctcttga aattgctagc aaatctggtg taagccattc atctttcact ttcgtactac
    12601 gaataactat tccttcggct ggcaaatcat ctattatttt gaaatattcc ccttgtaact
    12661 tttctatctc ttgttctgga atagcatcaa tcaaatgaac gtgatacata aaaccccctc
    12721 cttactaaca agtatctcga aatttctgca attagacaac tgtttaccat ttgacttcct
    12781 agaagcaaaa agagcagtta gagcagagta ggctgagtga taaaaataag gctatgacaa
    12841 gttactgcca taagccttat tttcgagagc aaaatattga gggtgagata tcttgataaa
    12901 tggtgttccg ttagctgaca ttgataataa gactaaattt caaaaaagtt gaaaagcaaa
    12961 tttcgtcaat ctcttcttat ttcttaaaac taagtggctt tttcacaacc tctttttatc
    13021 taataaccac tgtttccaac tcaactaatt ttgcccacgt ttcaatttga tctgttgtta
    13081 gatttagcga gacaacggta tgatgaccgc cacctgcctg aatccatgaa tggacacctt
    13141 catggaaatt cggtttagtt ttccataaca cacgtgcaac aggtaaatta ggtgcttcag
    13201 tcgttggttc aaaggcttct acttcattga tcagcagttt ataatgcgta ccgaaatctg
    13261 ccatcgatac gactacccct tctcctgctt tcccatcaaa tacaagacgc gccggatctt
    13321 ctctatctcc cattgataaa ggagaaacaa cgattcttgg tttcgtcgca gcaagcgtcg
    13381 gatctacttc catcatatgg gattccaaga ttgcttcttg gccactagct agctcgtaag
    13441 tataatcttc catgaatcct gtatctttcc ctcgagccat gattttcagc aaccgatcaa
    13501 tcgctgcagt tttccagtcg ccttccccag caaaaccgta tccttctgcc atcaaacgtt
    13561 gtacagctag tccaggtagt tgtttcattc catacaggtc ttcaaaatta gtcgtaaaag
    13621 cgttatagcc tccttcttct aagaaacggc gaataccgat ttcctgctga gcttgtactt
    13681 ttacatgttc ctcccatgtc ttttcttcat aatcaccata gtcgaaggta tacaattcct
    13741 tgtactcttc aaataattga tcgacttctt cgtctgatac gcgatcgatt acttgtacaa
    13801 gatctccgat accaaaataa tcaacagtcc aaccaaattg aatctgtgct tccactttgt
    13861 ctccttctgt gacacccacg tttctcatgt tgtcaccaaa acgagccact tttattccaa
    13921 aactttcatt ataggctact gcaacatcca tccaatcagc aatctctttt tggatctcag
    13981 gtcgtttcca gtagccaacg acgattttat tttgtttatt taatcgagca ttgatgaacc
    14041 catattcgcg gtcaccatga gcggattgat ttaaattcat gaaatccata tcgatcgttt
    14101 tccaaggaat gctttcgtta tattgcgtag ctaaatgaag caatggtttt tgtagaagct
    14161 ttgtcccgcg gatccacatt tttgctggtg agaaagtatg catccaagtg atcacacctg
    14221 ctacttcttc tcgataattg acctctttca tgatttttgt gatcgtatca gcagtaactg
    14281 ctaattcttg aaggcggatt ggataaggca attggccatt ttcattcagt tcatcgacca
    14341 tttcttgtgc atgtttcttt acttctcgca gtgcttcttc cccatataaa tgctgtgatc
    14401 ctacaacaaa ccaaaattct ttttctccaa tattcaacat aattagccac tccttttatt
    14461 tgttttgtcc gtaatacgca ttttttccgt gttttcgtaa ataatgttta tctaaaatcc
    14521 gttgaggcag ctcttctgaa aatgaattga gctgtctggt aaataaattc atcttgcata
    14581 cttcatctag tacgactgcg ttcattaccg cactttgcgc atctttcccc caagtaaatg
    14641 gaccgtgtcc gtgtaaaagt acccctggaa ttgctaaggg atcaattttg cgttctttga
    14701 atgtttctat gataactttt cctgtttcat attcgtaccc gctgtcgatt tcctgttgtg
    14761 tcaaaaatcg agcacaaggt accgatccat aaaaagtatc tgcatgcgtc gtccccatcg
    14821 caggaacatc caatcctgct tgtgcccaga tcgtcgccca tgtcgagtga gtatggacga
    14881 ttcctccgat ttgaggaaat gaacgatata aaacagcatg agtaggcgta tccgaagagg
    14941 gattgagttt accttcaatg atatgattgt ccaagtccac aacgaccatg tcgtctgctg
    15001 tcatctcttc ataactgact ccgctaggtt tgataacgaa atatcgtgtt tcaggatcaa
    15061 aagcgctcac atttccccat gtatatttga cgagtccgtg tttaggcaag tcaagatttg
    15121 cttggtatac ttcttctttc agttgttcca gcattttcta tcactccttt ttcttgcaat
    15181 tgatgaatcg ctgttttttc aatgtcgatc ccctcacggt aacgtctcat aaatatctga
    15241 tagcctgcta tttcttctgc tgaaggtgtg atggtgattc cttggtcttg tccaaacacg
    15301 tcttctgcca agaagttttc taaagacttc ccatgtcgat ccatcatata ggctgctaat
    15361 aacgcgattc cccacgctcc accttctccg gctgtgtcca tcactgtaac tggtgcctcc
    15421 atagcagatg ctagaatccg ctgtccaact tctggtgttt taaaaatccc cccatgcccc
    15481 actaaacgat caatttggac attctcagat ttcaatatat ccattccgat tctcattgca
    15541 ccaaaagcac tggctaaatg gatccgcatg aaattcgcta aatcaaaacg gctacctggt
    15601 gtacgtacaa ataatggcct tccttcattt actcctgtaa tgttttcacc ggaaaaataa
    15661 ccataagaaa gtaatccccc gcaatccgta tcgccttcta gcgctttgtt gaataatacc
    15721 gtgaataatt tatctctgct tatctccata cccaaagaat tggtaaattc ttcaaaaata
    15781 ctgacccaag cattgatatc cgaagagcag ttattcgtat gaaccatgcc tactagttct
    15841 cctgaaggtg tagtgaccat gtcgatttcc ggatggacat tttctagttc tttttctaat
    15901 acgatcatag cgaaggcgga agtaccagcc gaaatgttcc ctgtccgttt ggctacactg
    15961 ttcgtagcga ccataccggt tcctgcatcc ccttctggcg gacaaaccgg gattcctggt
    16021 ctcaaatttc ccgtagggtc gatcagataa gcccctgatt cgcttaactt gccagcttct
    16081 tctcctgcta ctagaacttt aggcaaaatg tgatccaagt caatcgctac gtgcgctttt
    16141 tcgattaact gaccaaaaat atttttcatt tcttgatggt aggttttcgt tgaaggatcg
    16201 ataggaaaca tgcctgatgc atctcctacg cctagtactt tctcaccagt aagctgccaa
    16261 tggacataac ctgcaagcgt tgtcaaaaaa ttaatttctt tcacatgttc ttctttattc
    16321 aaaatagatt gatacaaatg agcgatactc catctctgcg gaatattgta ttgaaatttt
    16381 tccgttaata ttcgttctgc ttcttcagtg attgtatttc gccaagtacg aaatggtact
    16441 agtaactgtt ccttgctatc aaatgctaga taaccgtgca tcattgcact gaacccaatc
    16501 gacccgattg tcgtaagatt ctcgccatag atttcgaata cttctgctgc catttttcgg
    16561 taactgacgc gcagtccttt ccaaatatct tctaatgaat aggtccagat accattttct
    16621 agtttgtttt cccactcata actaccagcg gcaataggag taaaagatga atcaatcaat
    16681 acagctttga tcctagttga tccaaactca atccctaaag atgtacgacc atctatgaca
    16741 tctaacatac tttgcgcatt tacttccttc ataatgattt aactccctcc ttctcgtcaa
    16801 acctgatcaa accgtctttt tagtagcgct tacaatgatt attgtatact tttgtacgta
    16861 catgtcaagc ggttatttac aagaatacaa taaaaaacac ttgttttgta cgtacataag
    16921 ttataaaatg aaattgctta caaaatctga tcaaccgcct tctcgaaatt ctagaaggag
    16981 agaatactta tgaaaaaaga aaaaaaagtt tcctcatcgt ttatttattt ttttggcgca
    17041 tttggcggca ttctatttgg atatgatatt ggtgttatga ccggcgcctt acctttttta
    17101 cagcatgatt ggggtttggc aggtaaagct tctttgatag gatggatcac gtcttcggtc
    17161 atgttaggtg cgatccttgg cggctcttta tctggtcttc tttctgataa gcttggacga
    17221 cgaaaaatga ttcttctttc tgcacttatt tttatggctg gttctgtcct ttctgcatct
    17281 gcccctcaca atggttctta ttttctgatt gcagcaagga ttcttctagg tttagctgtt
    17341 ggcgctgcat ctgccctagt tcctgcttat atgtctgaaa tggctcctgc tagactaagg
    17401 ggaagattgt cagggatcaa tcaagtgatg attgcttctg gcatgctcct ttcctatgtt
    17461 gcagattatt tgctaaaagt gttgcctgaa acgatggctt ggcgcgtgat gttagggctc
    17521 gctgctgtcc ccgcgctgat tttatttttc ggtgttttag ctctaccaga gtcaccgcgt
    17581 tttcttatgc aatctggccg cttggaagag gcaaaaagag tattgaacta tattcggaca
    17641 ccaaaagaag ctgaacagga attcgaacaa atccagctga atgtcaaaca agaaaagaca
    17701 accggaactt cttggcatac tttgttttta gaaaaatatc gttccttagt cattgccggt
    17761 attggtgtag cagtattcca gcaatttcaa ggagctaatg ctatttttta ctatattccg
    17821 ctgatcgtag aaaaagcgac tgggcatgca gcgagcgatg cattgatgtg gccgatcatc
    17881 caaggaatca ttttagttgc tggttctttg ctctttttgg ttattgcaga caagttcaat
    17941 cggcgaactt tattgaaaat aggcggtagc gtgatgggac tttcattcat cctaccagct
    18001 gttttaggaa ctgtactgga tgctcataca aattcgttgt tgattctctt gtttttatgt
    18061 atttatgtcg cattttattc ttgcacatgg gctccactga catgggtaat cataggcgaa
    18121 atattcccac tagctgtccg cgggcgagca tctggcctag cctcttcttt taattggatt
    18181 ggttctttcc tagttggctt gctttttcct gtgatgactg cttctatgtc tcaagaaatc
    18241 gttttcggca tttttgggat catctgcttt ttaggggttt tatttattca agagatcgta
    18301 ccagaaactc gtgggaaatc tttagaggaa atcgaacaaa gtgcatctaa aaaaacttat
    18361 cctaaacgta tttctatcca tcattcttaa agaggttgtg acagaagtgg tcagcttcaa
    18421 gaaataagaa ggaaccgctt agtttccagt cacaaggaac aatttcaaaa aacagcttct
    18481 catgtttgat gaatattgtg acttgtgacc agttggtagg agaacaccgt ttagtggaga
    18541 ggtggtgaaa gaaacgttca gccttgagcg atgacaaaag ctctcacaaa cattgcgacg
    18601 agtaaccgta ggagcaataa gaggcggtaa aaacccgtct cttatttttc gttttctttc
    18661 actgtttccc gttcaactag tacaggttca tagagaatat ttggctgtgc ctgtcctttt
    18721 tcaatcgttg tgacgatcca ccgtgcggca tcttgtccca acttttcttt tggatgcgtc
    18781 aatgttgtca attttttatt ccctaaatgg cttaaagaag aatcgtctgt tcctacgatc
    18841 gataggtcat ctggaatatg atagccattt tctgtcaact tatctagcat taaactagct
    18901 atttgatcgt tatagcaaat gatgccatct aaagcttgtt cttctttcaa ccgttgtact
    18961 gttttttcag cgattgtttc tcgtgtttcc gtggtgaaag taatgatatc ttccgtcaaa
    19021 aaacgcacat tatactgttc acacgcgctg ataaaacctt tcatcctgta ttttccttga
    19081 agatcgtcga tttttgtgat gaacaacaga cgctgatgtc ctttttttat caaatattct
    19141 gctgccataa aaccgacttt cacatcatct aaacaaatat aagaggcatt taactcttca
    19201 taagcagcat tgatcatcac catcggaatg ccttgttctc tcaaagcaac ataaagtgct
    19261 aagtttgggt tatactgatt acttttcgta ggctcaacga tcaaaccatc taccccataa
    19321 gcaatcattt tcttcaaaca ctctttctcc aattcgtgat cgttattcgt actggctaaa
    19381 agcaatgaat acccagattc tcgtaattca ttttcgattc ctcggataat cgacggaaaa
    19441 atataatcag agagatatgt ggtgatgaca ccgatcgttt ttgttctgtt caatggtgtt
    19501 ttttctttct tatagctgtc atctacatat gtgcccgacc ctttttcttt acgcagatag
    19561 ccctcattta ccaacacagc gattgcttgc cgaatcgtat gtctgctgac gtcatatatt
    19621 tcttgcagct tgaattctga gggaatggtt gtatttactt cgtattcctc acgaaggatc
    19681 ttttgtttta tctcttctgc aatatcttga tattttgcca tttgccttgc ccctttttat
    19741 ttgtacgtac atatatcata acgagtctgc aaggtttatt caagcaaaaa tcaaggtttc
    19801 ttgtgctcat aaggcatacg gaacaggttc accaaattct ggaaatcctt gctcattcca
    19861 cgtaaagcat tgcgcgttcg cgtgccgatt aggattgtct aacggatccc ctttttggtt
    19921 cttctctggt cgagcatgat agatcaaaat atcaaagcac ccgtcttctg aaaccgtaaa
    19981 cgagttgtga ccaggaccaa attgcttatt tttttcagaa gaaacgaata ctggcttcga
    20041 taatttatgc catgattctt ttaataaaag attcttgtgc tcatcagccc acagcaggcc
    20101 catggcatag ttttcatctg ttgcactacc agaatacgta atgaatattt tcccattgcg
    20161 agtaaggact gctggacctt catttactga aaaaccccgt ttttcccatt catactcagg
    20221 tatagaaagg aggatctgct tcccctttaa cgtccaagga ttatccattt cggatagata
    20281 aagattagaa ttcccagtaa tttcaggatc ttgttgggcc cacacataat atagctttcc
    20341 ttgagaagag aaagttgttc cgtctaaact gaaagtttcc cttttcgttt ttatttgtcc
    20401 cttttctatc caatctgtat tcattggatc tatatgctga ttttcaagta cgaacatgcg
    20461 gtgatgatga gattcatcac gtatagtttt atgattgctt gcagcaaagt aaatatacca
    20521 ttttccattg atataatgga cttcaggtgc ccagatcaat tcactcatcg gtccttcttc
    20581 atgagcctgc catacaatca ggcttgcagc atgttctaat tcattcagtg actttgccct
    20641 tcttaattca atcacttggt agcctggaac cgatcctgta aaataataat aaccatctgt
    20701 atgtttatat acccaaggat ctgcccgttg ccgaacgata ggattattat aaagtgtctt
    20761 catcataaca aatttcctca cattttcttt attctaatta cattccaaga gtatttttct
    20821 atccctacat ttaattcgtc atgtttttct attgaaaaat agtttatttc aggttttatt
    20881 ttttctggtt cttcacgtgt atttttgtcc aacagatcgc tactggttaa tgtgtagtgc
    20941 tctccttgat ctagcaaatt gaaatcatgt tcaatcgtta tcttttgttg ttcatttgtg
    21001 cgattgatca agaaaatagt cagctgattg ccgttatcta cgatcacact gtccacataa
    21061 ggaacatttt ttatcttttc acaatcataa acagggcaaa tgatttgttg tttcaatact
    21121 tgacctttag catatttcga taagtgttgc atcacataat aagttggatt cctccatgct
    21181 tgtcctttag catctgtcag tattaaaggt attgtgttta ctaacaatga ttgacaacta
    21241 atctttacgc gatctgcgtg tcgtaaaata gccagcccta atgaaccaaa taataatgta
    21301 tcttccatag taaagtaaca ccaatcgatg ggactaccga cttcaaaatc cctatgtctt
    21361 ttttccggtt gggcatgtac attccattca tcaaacgcta gatacattgt tttctcactt
    21421 ctcaaatatc cttttacata atcacaagta gcaacgattt cttcaatttg acgatcgaat
    21481 cgataagctt ttgcaaggta tgtcgctgta tcatcacgct catttttagg aggtttagtc
    21541 aagtcttctt catcatactt atcaatatag ttatgcaaag ctaaataatc tacattctca
    21601 taagcttctt ctaatgcgat acgatcccat tcagggtacg atggtaaacg tggcgtagag
    21661 cttccaacta agattgtttc aattgaatca tccactaact tcatgacttt tgaggcttca
    21721 tttgctaacc taccatattc ataagcactc ttagccccca cttgccatgg accgtctagc
    21781 tcattcccta aacaccataa tttaacgcca aaaggctttt tttgcccatt ttttattctt
    21841 aaatcactcc agaaggttcc ttttggaaaa ttacaatatt ctactaaatt ttttgcttca
    21901 tcaattcctt ttgttcctaa attcaccgtg aatatcggtt tcgcttgtac atgactgatc
    21961 catttaaaaa attcgtggag tccaaaagaa tttggttcta tacctcgcca tgctaaatca
    22021 attttttttg gtcgttgttc tttcctacct acagtatcca cccaatcata accagatgta
    22081 aagtttcctc caggatagcg aatgacacct aattctaatt cctttacata agagaggaca
    22141 tcttgtctaa acccgtcacc atcagctgtt tcatggcccg gttcataaat ccctgtatag
    22201 acgacacttc ccatatgctc aacaaatgag ccaaacagcc gagaactgat ctctcctacc
    22261 gtaaaatcct ctctgatttt tactttcact tcttcgtcca acagctttcc ctacctttca
    22321 tttcctatag tcaagataat agatacaagt ttccatacgc caccagcaaa gataaaaata
    22381 aaaggaggga aaagaaatat gagcagtaca aatcctaata acaatacgca acaaagaata
    22441 tttctcaaaa aatcaaaaaa tactttccat gcactttgtt caaaaatttg taaaaatgtg
    22501 atatctttgt tttcagaaat cacatatatc caggataaaa gaaacatgga ataaataagt
    22561 aaaactacat atccactaat tgaaagtacg attgtctgtt cttctaataa ttgattaaca
    22621 agaaaataaa taatgatcgt taacagacta aggaacaagt tcttaccaaa atgttgcttt
    22681 attccttgaa agaaaaaata aatgatattt cctgatccct cacctttcca ttgcgccatt
    22741 acataaaaaa cagcacatgt agcaggcacg atagtaataa caggcaaaca cacgattacc
    22801 cagagaatac ctaataagat gtaatcagaa accacaatca actgtccata aaatttagtc
    22861 tcaactattt tcatcttcct cactctttca ttccagacat agctgttcct tgaacaaaat
    22921 atttctgtgc aaaaatataa accactagta atggcagcat tgaaataacc gttccagcca
    22981 ttacaggtcc ataaaaagaa atgttctgcc ctgtaaataa agctaaccct gctggtaaag
    23041 ttctcatttt tgaactactt gtcaatatca atggataaag caagtcattc caactattca
    23101 tcagcgtgaa aatccctaat gataaaagag taggtttgct caatggcaac atgattttca
    23161 aaaaaatatt aaactcactt aatccatcaa tgcgtgctgc ctcttccaaa gaatctggta
    23221 aagtaataaa aaaggaacgc atcatgaaga taccaaatgc agttgtcata cgaggaatga
    23281 tcaaacctgc ataagtatca agcatgccaa gcaaattgac ttcaataaat agaggaatca
    23341 tgaagacttg gaatgggatc atcattgtca gcaaaacgaa atagaacaaa actgattttc
    23401 ctttgaaacg catacgagca aatgcatacc ccgcaagaga atcaaagaat acagaagtga
    23461 tcactgtccc tccagcaaaa atcactgtat ttttgatgta atctaataaa gggatcgttg
    23521 tccaaacctt aatatagtta tctagggtat agctatctgc aaatattcta ggcggaaatg
    23581 agataatgtc tttttcgtgt ttaaaagaag aaataatcag ccacaaaaaa ggaaacgcaa
    23641 taaccaacaa tataactagc atactaatcg ttcgaatgat ttttttacca ctaactgctt
    23701 ttcgactatt cacagtaact gtaatcgagc tttctggtgc catacaggtt tccctcctat
    23761 ttcatttgtt tttctttccg tgacatgtac acattagtgg agatagctat agttgcaaca
    23821 atgataaaca aaaccgaaga cagtgctgat gcatagccca attcataagg tgctgtaaat
    23881 cctcgactgt aaatatattg aacagctgtt tctgtcttaa attgtgggcc accagcggtt
    23941 gcaacatata cttggtcaaa cacttgcatc gaaccaatca gattcgttaa aatacaaaaa
    24001 cctaaggagg gaacgatctg aggtagcgta atgttaaaaa actgctgtcg atttgttgca
    24061 ccatccattt gagcagattc atacaaagag ggagagattc cctgaatagc agtcaagaga
    24121 atcgtcatag taacaccaaa attcttccaa acagtcatga tggctaccgt cggcatagca
    24181 agggttggat cgcgaaaaaa ttgagggatc ggcaagccga cttgtgaaaa caagtaagga
    24241 atcgccccta cattcggatc aagcaacata gaaaaaataa tcgcaattga cgtcaatgaa
    24301 caaataactg gaatgtaaaa tgtcgaacga caaaaacgat tgaatctggt atttttagat
    24361 aatgctactg ctgctaataa accaacgata atttgtgttg gcgtttcgag tacggtgaaa
    24421 tataaagaat gaatcaacga attaactgct cgttcatcac taaatatacg tgtgaaattt
    24481 tcaaaactta caaaatttct tgatgtaaag aaaatattca aatctgtaaa actaatagca
    24541 aatgttccta aaagcggtat gacagtaaat aataataaaa ctaaaaaaga tggagttaaa
    24601 aacaaatacg cagcacgatc aggacgattc caatatttgg tgaatccgtt catatagtcc
    24661 acctcatctt tcattttgat aaaatataca atcttggttt aaacgaatct gaataagata
    24721 tccgaacaat cgctgtgatt tttgcaataa cacctatctg caaatactag gtcgatcatt
    24781 cagatatctg tatcttgcca acagtctatc ctgtataaag ttctgtacta tttttgcagt
    24841 aattgattga tggcatcatc tgcatcgttc atgagtgtct caggatcttc tcctgctaat
    24901 aatttttcaa tcagtggatt gatgatatcg ttattgatcg cagagatttg ggtaaaacct
    24961 ggtaaatatg gctctgcata gtccatttgt ttcgataatt cagatacaat aggatcattt
    25021 tttacttctt catcttttgc tactgaatcc aagtaggcag gaaaaccatt ttcgatactc
    25081 cattttttac caatttcagt tgtattccaa tatttcaaaa actcatagac agcttctttt
    25141 ttattattat ctgtacttgc cggaatggca tatcctacgc catctaaaat agctgttttt
    25201 ttgtctgaat cactcccttg tggtaaggta gtcacattat aatcaatctc gtttgctttc
    25261 aatccgttat ttaaccatgg cccattgatt tcaatggcta gttgaccagc attcattaaa
    25321 ttgtccattt cggcgccaga aatcgattct ggaccagtct tgtctacatg gatcattttt
    25381 tggatcgctt ttaacgtttt aaggttctca ggagacgcaa attcagactt agtataatca
    25441 tcgttcacta atttaccgcc atttgctaac atccagttat ataacggtgc agttccatcc
    25501 ttattgaaga caaaacctga tacattttta ctactatcag ttaattttgg tgccatttct
    25561 gtcaattctt cccaagtagt aggtggttgt tcagtatcta atccagcttg tttaaacaaa
    25621 tcattattcc aatacatata catgccttgt acctgcatgg ggacaaaata ctgtttacct
    25681 tctacttcac ctaattcaat agccgtttct ttaaaatcag atttatctac tccatcatat
    25741 tcccagaaat cgtctaatgg ttgtaccgct ccgttttcta catactgtgc aaaatctgga
    25801 taatttaatg caataaagtc aggtgctttt ttagaagaaa tcgctgtagg aagtttttca
    25861 ttcaaattgg cccaagtcat gatatccatt tcaattttga tgtttttatc atttgtttta
    25921 ttaaattcat taacgatttc ttgcaatatt tcaccatcag atgcagtaaa cccattccaa
    25981 aaagtcaaag tcacttcttt atcagaatca gattctgctg aacttccgcc accgcaagct
    26041 cctaaaatac ctgttgcagc taccaacaaa gagccaatta accatttttt acctttcatt
    26101 ttttgttcct ccttcatctg ataaacgaca tctgaccaca ttccaagata gcggtttcaa
    26161 taaacaattg attttcccat cttcagcttt tacatttgtt gaatgacttg gttttacttg
    26221 ttcactttta ggaagattag ttgcttttgt atcaaatcca ctcatttcag aaaaatcaat
    26281 cactccctct aatgataatt cagacaagtg aacagttaga ttcatttctt cttctccacg
    26341 gttaactgca aatatgacaa gttctttgtc atttattacg gctatacttt ctaaatacgg
    26401 cacttcttca aaatcttccg tcgcatagtt tgctgaacga atttgtgggc ttaacacctc
    26461 tccttgtcca tacacagcag cttgcataaa tggataatag atcgtctgtc tccaagcttc
    26521 tccgtttgtt tctgtcctga ttggtgcaat aacgtttacc aattgagcaa gacaagcaat
    26581 ctttactcta tctgcatgtt tcaataaagt gattaataga cagcctaata gtaatgcatc
    26641 ttcaaaatta tagttatctt ctagtaaagg aggagcaatc tgccaaggcg ctgcttgctc
    26701 atcttgttcc gctgaatgat accaaatatt ccattcatca aaggacaggt tgatctgctt
    26761 cttactatgt ttcttcgcct tgactgtatc gcacatcgct gtcacttcag aaatgaaccg
    26821 atccatgtct aaggaacgtg caagataatg agaaagatca tttttttggt tgccataata
    26881 ttgatgcaga gacaaataat ctatttgatc gtacgcttga tcaagaactg ttaactccca
    26941 atcaccaaat gtaggcattt gccgattcga acttccgcat aaaaccacct caattgaatc
    27001 atctacgagt ttcatcgcct tagccgtttc ctctgctaac cgtccatatt cttccgctgt
    27061 tttatgtcca atctgccaag gaccgtccat ttcattccct aaacaccatg tcttaattcc
    27121 aaaagggctc tcttgaccat tcgaacgcct taaatcactc caataagttc ctttaggaaa
    27181 attacaatat tcgactaaat tacgagctgc atctactcca cgagtaccca gattgacagc
    27241 catattgatt tcggccccca catgctgaca ccagttataa aattcatgaa tcccaacttc
    27301 attcgtttca attgtccgcc aagctaaatc caaccttcta ggtctatttg ctatgggccc
    27361 aatactgtct tcccaacgaa atcctgacac aaaatttcca ccaggatatc taatcatggg
    27421 aatgttcaat tcttttacca agtgaatgac gtcttggcga aatccacttt tattagctga
    27481 tggatgatct ggctgataaa tcccttcata tactgctcgt cccatatgtt caataaatga
    27541 actgtacaac cgcggatcaa ctttgcctac acgcttgaca ctactaatca acattgtggc
    27601 ttccataact gacctccttt aatttataca tgaataatac acctcgttga aagcgttgtc
    27661 aataatataa atgttaaact tacttatttt ttatcgattt taattaacca aaatattaat
    27721 aatgattaaa acaaataatt taatataact tttattaaca aatatgttaa tatataagaa
    27781 aaacctttta agaggtgata tatatgcaac tacaaataaa cgaagaatcc ttaccggtat
    27841 atgaagcatt agctagtaaa acaaggatca agatcatcca gctactttcc aaaaaaaaga
    27901 tgaatgtcaa agatttggca aaagaacttg gagtgagcag tgctatcacg acgatgcacg
    27961 tgaaaaagct agaggaagca aacatcatca aaacagaaaa agtaggacaa caaaaaattt
    28021 cgagccttcg tgtcgataaa atcgatatta gttttccaga aaaaatattc aatgcttttg
    28081 atacgaaaga aacatcgata cctattggtc actatacaaa ttatgcaatt gaaccaacat
    28141 gcggtttagc tacgatccat gattttatcg ggaaagtgga tgaaccacgc tattttatgg
    28201 atcctcgaag gatggatgcc cgcattctct ggtttaccag cggatttgtc gagtatcagg
    28261 cacctaattt tttaaataca gaggatacat tggagatgtt ggaagtttct gtggaaattt
    28321 cttctgaatt tcctttttca aatgacaatt ggccttcaga tatcactttc agcctaaatg
    28381 gtgtggagct aggaacatgg acgagccctg gaaattttgc tgatatccgc ggtaaatata
    28441 cgcctgattg gtatccagat aatcttaatc aatacggctt gctgaagacg atccgaatta
    28501 ccaaacactt gacgaatatg aatggagaac ccttgtcaaa catcacgatc aacgacatcc
    28561 ctaaagagca agacacttgg catttgcgaa tcgaagtgaa agacgatgcc aaacatgtgg
    28621 ggggctgtac tttatttggt aaaggattcg gtaattacga tcaggatatc aaattaaaag
    28681 tttactacag ctgatatttt tcttttctat tttataaaac aaaaacagcc aatccctatg
    28741 aagggacggc tgtttttgct ttattgttat ttccagaaat catccaaaac ggaaattggc
    28801 aaatgtcgtt tatgttgtgt cttgttccac catgcttcga ttttttcttg tgcttgagcg
    28861 ggaacttctt ttccttctag ataatcatca atttcattat atgtgacacc tagtgcgact
    28921 tcatctgcaa tcattggttt cccatcttca agatctgcag tcggaatttt tgtgtataat
    28981 ttttccggtg cattcaatgc ttgaagcaat tgtttccctt gtcgtttgtc caaacggaat
    29041 aatggcaaaa tgtcggctcc accatcacca aatttcgtga agaatccagt aatgttttct
    29101 gcagcatgat ctgtccctaa tacggctcct gctctttctc cagctacggc atattgcgta
    29161 atcatacgtt gacgtgcttt gatatttcct ttgttaaaat cgctgattcc cacacctgct
    29221 tcgcttaata cttgtacttg tgcatcaaca gctggtttga tatttactcg cagactaaca
    29281 tcgggctgga taaagtcaag agcagctttt gcatcttctt catctgcttg ttcaccataa
    29341 ggaagacgaa cagcgataaa ttgatagttt tcatcttttg tttcttcacg catttcttcc
    29401 atcgttagtt gcgccaaacg gcctgctaaa gtagaatctt ggccgccgct gatacctagt
    29461 acaaaagttt taagaaacgg atattttttc aaatatgctt tcataaagtc aatactttta
    29521 cgtatttctt cttttggatc aattgttggt ttgacaccta attcattgat aatttctgtc
    29581 tgcaggctca tttattctcc tcctatgctt ctgtatgaag ttctgctacg ctttttttca
    29641 ttcgttccat gatagccatt ttatggttcc agcattcagt agacagatcg actgggtatt
    29701 tttgcggatt caagtctcgt ttatactctt cccatagtga atctagattt tcttttgtat
    29761 attctttgat ttcgtttagt gcaggtaatt cgtaaactct ttttccttcg ataaagatat
    29821 cttgtaaaac tgggcgagct gtaaagtctc gaacagtttt attgataaac gtatgcactg
    29881 gatggaacat gaagatagct tcttcttctc gagggtcttc atcccataat gtcacgtagt
    29941 ctccttcaga tttcccatca aaatttcgag tgatgcgcca cacttgtttt ttccctggag
    30001 tagttacttt ttctgcattg cttgataact tgatcgtatc catcattttt ccttcatcat
    30061 cttcgatcga tactagcttg aatactgcac caagggctgg ctgatcatag gctgtgatca
    30121 attttgtccc aacaccccat acatcaattt tagctttttg catctttaaa ttcaaaatcg
    30181 ttgcttcatc caagtcattc gaagcataga tttttgcttc cgtaaatccg gctgcatcta
    30241 attgttcccg aacacgctta gatatatagg ccatatctcc actgtcgatg cgtacgcctt
    30301 ggaagttgat tttgtcacct aattctttcg ccacgcgaat cgcacttggc acacctgatt
    30361 tcaacgtatc ataagtgtcg actaagaaca cgcagtcttt atgggtttta gcgtaggcca
    30421 taaatgcttc ataatcattt ccataagatt ggactaacga atgagcatgg gtcccgctag
    30481 ctggtatccc aaatatcttc ccagcacgta cattgcttgt tgcatctgct ccgccgatat
    30541 atgccgctct agttccccag actgctgcat ccagttcttg tgcccgtcta gttccaaatt
    30601 ccaatagtgg atcttctccg atgacagatt tgatacgagc tgcttttgtc gcgatcaacg
    30661 tttggaaatt gaccatattc aacagtgctg tttctactaa ttggcagtgc gctaaaggcc
    30721 cttccacttg gatcaatggt tcatttgcaa aaactagttc tccttcacga gcagaacgaa
    30781 ctgttgcttt aaattcaaaa ttttgtaaat actccaaaaa accttcagga tagacttcta
    30841 atccacgcaa gtaagcaata tcggaatcat tgaacgtcag attctctaaa taattgacta
    30901 accgttccaa tcctgcaaaa accgcgtatc cgtggttaaa aggcatttct cgaaagtaac
    30961 attcaaaaac agcatgtaaa tcagctcgac ctaattccca gtatgtctgc atcatattga
    31021 tttgatatag atccgtatgc agcgtcaaac tgtcgtctgg gtaagtatta ttcatcttgt
    31081 attactcctc ttggcaagtt ttcatttcct acccattata acaaaaaaaa ttaataggaa
    31141 aagaaatctt ccattttttc tgaaaaagca catgttttac ggatatttct tgctaaaaat
    31201 gaactccatc tgctaaaact atcctttctt attggtattt aacctaaacg agaaagaata
    31261 cttattttat tcaatgtatc cgagacacga agaaattagc gttttctatt tttcttactt
    31321 gataaaataa gtagattttc ctactacaat cgtagccgtt aagataccga tgattgaaca
    31381 aatataacca aatgtctcta caacataatc aatggcaaaa gtttttgaag caataatacg
    31441 tctttccttt tttgcttctt atccccaaag aaacggatat aacttattat gtattgaatt
    31501 tttttggaaa ggcgaattat gacaagatat accttgcgtt gaaaaaactc gcggaaaaaa
    31561 tatcacacgt ttttaaaaag atacaaagta agaaagcagc tgtcaataga atggaaacaa
    31621 aaatggcgag acatcaatca gtctcgcctt gttttttatt gtatacatta gatatggagc
    31681 aggttaccat aaagatgatt attagtcgtt attaaataga atccttcgta tttttttgtt
    31741 ctatcactac aatatcttta tttggacgtt tgaattgtat tccggcacgt tctagctcct
    31801 gtttcactcc tacatggact ttgacatctt ctcgtgtcat ttttaaaacg tgatgaatcg
    31861 catagtcgat ttcttgcctt gtgatttttt tcttcgtatt gatcatcacg acttgctttt
    31921 tatcatatgt gtctaccaat agcagttgtg ttcgatctaa cgaaaataat ttgtaataat
    31981 taatagccgt attcccaaaa aagaattcca ctactttagg ataaaatgtc ttaatattta
    32041 aattactatt cacaggtgtt tcgattagct tcatctcatt cctcttttct atatagtcga
    32101 tttagatcca atcttaagtt atgttaccat ctttcctagc attaaggaat cggttacaat
    32161 tgaaccttta aagacaaaag gatttacctt ttatcactta atagtataac ataatttcat
    32221 gagaaaaaaa tacaattttt tcttgaccat ctcgttacgt aaaggattat gcttacattt
    32281 gtcaggagga aaaataatgg agtacacaat aaagaaaatg tcagagatat caggagtcag
    32341 tccgagaacc ttgcgctttt atgatgagat tggactgcta aaaccagcta ggatcaattc
    32401 ttcaggttat cggatttatg gcaaaaaaga agtcgatcgt ctgcaacaca tcttatttta
    32461 tcgaacaatg gcattcaagt tggatgatat ccaagaggtc ttagacaatc catcttttga
    32521 ccaccagaaa gctttaatca agcatcagca aatgttgctg gaaaaacggg cacaaatcga
    32581 taccctcttg acaactgtcc aacagacact tgatatgtac gaaggaggaa gaaaaatgag
    32641 tgacaaagaa aaatttgaag ggtttaaaca acaaaagttg aaagaaaatg aagaatccta
    32701 tggacaagag attcgacaaa aatacggaaa aaaaaaggtt acagcatcca atgaaaaatg
    32761 gatgaatatg gataaagaaa cgtatgaaga aatgcaaact gttgaaaagc agatgttatt
    32821 tgatctcact ctttacttaa aagaaccaga agatgaaagc ttagcagacc gtatcttcca
    32881 agctcataag aagtggttat cttacagctg gcctgaatat cagccaaaag cgcataaagg
    32941 actaggaatg atgtacgaag ccgatgaacg gttcactgca tattatgacg agcgaagtgg
    33001 tcatggtgca gcaaaagctt taaatgaaat tatccaaaaa aaggcgtaat cttttacatg
    33061 atccaacaaa aaagtacacg taaaatgacc taataacaaa acagcagaca cccaagttag
    33121 atttttatgc taacttttgg tgtctgctcc attctttcac aagttttttc atcctacttt
    33181 aaatatattc atcgttgaat aaacgtataa ggtattttca ctttatcctt tttttctgta
    33241 agaaacaatt gaaatgccat ttctccgcaa gcttccagat ggtggtcgat ggttgggaaa
    33301 ccaagaagct cgctgatcag caaattatcg cgtccaatca ccactggtgt cttatcttgc
    33361 agatagcttt gcaagatgcc tgctgcaacc atatccgaat ttgtaaagac accatcaact
    33421 ccaagctctt taaaaaagaa gcctgcttgt tttcctttct ctgcatctat acaatcccaa
    33481 aaaacaagtg attcatcaaa agatggaaaa agttgatgac atagctgcaa agtgattttt
    33541 gagttataac ttagcctgcc acttcgcccc aacgtgatgc ccaattgctt tataccttgc
    33601 tgcttcaaat aagtaagacc ttctgtaatt gattgttctc gatcaataaa gacacagctt
    33661 gcctgttttc ccttgatttc ttcacaaaaa actagaggtc catatttttg atatgccaat
    33721 aaatcatcta gtgtattggc acgagacgta atgatcaatc catcgaaagc tttagctgca
    33781 aattcttcaa gataccttcg ctctttttct ttctgatagc ccgtcggtaa aagtgtcact
    33841 ttgtaatcat aatcaaacgc tgcttcaatg atcccactga tcaactgatc aaaatacgga
    33901 tgattggtat aaggcagaat cactccaatg cttttcgttt ttccataact taactgtctg
    33961 gcatttgcat tagggatata atcgacttct ttcatgaccg caagtatttc tttccttttt
    34021 tcctcatcta catatggatg tccattgatt acacgtgaaa tagtagaaac tgaataacct
    34081 gtgagctttg cgatatctcg aatgtttgcc actgctgctt cgccacctat acaaaaagat
    34141 tttcttgtaa atagtctacc acagctggac cgcattcaaa aaactgatac atttttcctt
    34201 catgcgaaaa actgatccgc tgcccgcccc aatatatatc ttttgtcaaa ttttcgattt
    34261 cattcattgg tatttctttt actatattcc tattcaaccc ttgaaaactg tacaacgtta
    34321 atacggatcc cttgaactcg acaaacgcat aaaaataatt gttcgttgtg ccatctgtta
    34381 tctctacata agtattcatt gcaagccctc cttttgatac tttgagtata gtgtatgaaa
    34441 cgcgtctcat agcaaggagt ttgttttgcc aaagaaaagt tttattcctc tttcattatt
    34501 accaaagtct cttattttaa attgaataca ttataatgat actgtaaata tatctttatt
    34561 attagtgaat ggatattta
//
LOCUS       DAZKKW010000025        32168 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.025, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000025 DAZKKW010000000
VERSION     DAZKKW010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32168
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.025"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(105..485)
                     /gene="yajC"
                     /locus_tag="ACG8PO_001140"
     CDS             complement(105..485)
                     /gene="yajC"
                     /locus_tag="ACG8PO_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFQ0206482.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(582..1727)
                     /gene="tgt"
                     /locus_tag="ACG8PO_001141"
     CDS             complement(582..1727)
                     /gene="tgt"
                     /locus_tag="ACG8PO_001141"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFQ0206483.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            1945..2643
                     /locus_tag="ACG8PO_001142"
     CDS             1945..2643
                     /locus_tag="ACG8PO_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HFQ0206484.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(2811..5450)
                     /locus_tag="ACG8PO_001143"
     CDS             complement(2811..5450)
                     /locus_tag="ACG8PO_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFQ0206485.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(6069..7100)
                     /gene="queA"
                     /locus_tag="ACG8PO_001144"
     CDS             complement(6069..7100)
                     /gene="queA"
                     /locus_tag="ACG8PO_001144"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFQ0206486.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            7293..8468
                     /locus_tag="ACG8PO_001145"
     CDS             7293..8468
                     /locus_tag="ACG8PO_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HFQ0206487.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            8472..9113
                     /locus_tag="ACG8PO_001146"
     CDS             8472..9113
                     /locus_tag="ACG8PO_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0206488.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            9110..10027
                     /locus_tag="ACG8PO_001147"
     CDS             9110..10027
                     /locus_tag="ACG8PO_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HFQ0206489.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYSFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            10024..10695
                     /locus_tag="ACG8PO_001148"
     CDS             10024..10695
                     /locus_tag="ACG8PO_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0206490.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(11236..11724)
                     /locus_tag="ACG8PO_001149"
     CDS             complement(11236..11724)
                     /locus_tag="ACG8PO_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HFQ0206491.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(11925..12191)
                     /locus_tag="ACG8PO_001150"
     CDS             complement(11925..12191)
                     /locus_tag="ACG8PO_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFQ0206492.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(12191..12454)
                     /locus_tag="ACG8PO_001151"
     CDS             complement(12191..12454)
                     /locus_tag="ACG8PO_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFQ0206493.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            12975..13115
                     /locus_tag="ACG8PO_001152"
                     /pseudo
     CDS             12975..13115
                     /locus_tag="ACG8PO_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            complement(13257..15080)
                     /locus_tag="ACG8PO_001153"
     CDS             complement(13257..15080)
                     /locus_tag="ACG8PO_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFQ0206494.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(15678..18383)
                     /locus_tag="ACG8PO_001154"
     CDS             complement(15678..18383)
                     /locus_tag="ACG8PO_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip family protein"
                     /protein_id="HFQ0206495.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(18689..19636)
                     /locus_tag="ACG8PO_001155"
     CDS             complement(18689..19636)
                     /locus_tag="ACG8PO_001155"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HFQ0206496.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            19826..20095
                     /gene="rpsN"
                     /locus_tag="ACG8PO_001156"
     CDS             19826..20095
                     /gene="rpsN"
                     /locus_tag="ACG8PO_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFQ0206497.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(20271..20633)
                     /locus_tag="ACG8PO_001157"
     CDS             complement(20271..20633)
                     /locus_tag="ACG8PO_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFQ0206498.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(20644..21765)
                     /gene="alr"
                     /locus_tag="ACG8PO_001158"
     CDS             complement(20644..21765)
                     /gene="alr"
                     /locus_tag="ACG8PO_001158"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFQ0206499.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(21781..22131)
                     /gene="acpS"
                     /locus_tag="ACG8PO_001159"
     CDS             complement(21781..22131)
                     /gene="acpS"
                     /locus_tag="ACG8PO_001159"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFQ0206500.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(22261..23772)
                     /locus_tag="ACG8PO_001160"
     CDS             complement(22261..23772)
                     /locus_tag="ACG8PO_001160"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFQ0206501.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(24094..25446)
                     /gene="murF"
                     /locus_tag="ACG8PO_001161"
     CDS             complement(24094..25446)
                     /gene="murF"
                     /locus_tag="ACG8PO_001161"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFQ0206502.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(25599..26675)
                     /locus_tag="ACG8PO_001162"
     CDS             complement(25599..26675)
                     /locus_tag="ACG8PO_001162"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFQ0206503.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            26871..28616
                     /locus_tag="ACG8PO_001163"
     CDS             26871..28616
                     /locus_tag="ACG8PO_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HFQ0206504.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(28896..29333)
                     /locus_tag="ACG8PO_001164"
     CDS             complement(28896..29333)
                     /locus_tag="ACG8PO_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HFQ0206505.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(29317..30690)
                     /locus_tag="ACG8PO_001165"
     CDS             complement(29317..30690)
                     /locus_tag="ACG8PO_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0206506.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(31012..31500)
                     /locus_tag="ACG8PO_001166"
     CDS             complement(31012..31500)
                     /locus_tag="ACG8PO_001166"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0206507.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEVFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(31522..32124)
                     /locus_tag="ACG8PO_001167"
     CDS             complement(31522..32124)
                     /locus_tag="ACG8PO_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFQ0206508.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT         9195 a         6878 c         5940 g        10155 t
ORIGIN      
        1 aatattgagc aatatatccc atgctcttat ctctttcttt tttgaataaa aaatgaggta
       61 ggacatcttt ctgccctacc tcgtctttaa ataagctttg atacttattt ttcttccttc
      121 tcttctgtct cttccagcgt ttctgttgtt tcagttttta cttcaactgt ctcagctggt
      181 tcttctgttg tttcaacagt agtcgttgca tcatttgtta cgacagtccc tggtttgacc
      241 gttttgattg ctgcacgatc gtactcaagg aagatccctt cgcagtctaa aacaactgtc
      301 cgtttgtcat tgtcgatttc tgaaacaact ccatgtaatc cgccgatcgt tacgactgaa
      361 tcacctggtt tcatgctatc taacaagttt tgacgttctt gctgttgttt cttttgcgaa
      421 cgtgacataa aaaaccacat tgctaatagc gcgataaaca tcacaagcat tggtaatgtt
      481 cccatttttt tcacctcact aattctgtat ctacaaaaaa cactttagca gaaaaatgct
      541 gataacacta gttttttact taatttttaa aagattttcc tttagaagct ttttgcgttt
      601 tctttattga acccgtattc ttcaaaaaag gcttctctaa attcaagcaa attatcatcc
      661 atgattgctt gacgtacttg tttcattaga ttcaacaaga agtataaatt gtggtaagaa
      721 gtcaaacgaa tcccaaatgt ttcatcacat ttgatcaaat ggcggatata ggcacgtgta
      781 tagtttttac atgtataaca atcacatttc tcatcaattg gacggaaatc atgggcatat
      841 ttcgcatttt tcactactag acggccttga gaagtcatac atgtgccatt tcgagcgatt
      901 cgagtaggca agacacagtc aaacatgtct actccacgga taacaccatc gatcaatgaa
      961 tcagcagcac cgacacccat caagtaacgt ggtttgtttt cggggatcaa cggtgtagtg
     1021 aattctaata cacgattcat ttctgattta ggttccccga cagataaccc gccgattgag
     1081 taaccaggga aatccatccc gactagatct ttcgcgcttt gacgacgaag atcttcaaac
     1141 cctgctcctt ggatgattcc aaataatcct tgacggtctg gattagcatg agctttcaat
     1201 cctcgttctg cccatcgaga cgtacgttct actgattttt tcacgtagtc gtaactttca
     1261 tcgaatggtg gacattcgtc aaaactcatc atgatatcag agcccaattt attttggatg
     1321 ttgattgctt tttctggtga taaaaacata cgcgaaccgt tcaagtgatt tttgaaatgc
     1381 acgccttctt cttcgatatt ccgcatatca cttaatgaaa aaacttgaaa gccgcctgaa
     1441 tctgttaaaa tcggttgatc ccagttcata aatttatgca gtccgccagc ttcttctact
     1501 aagtcttcac cagggcgtag ccataaatga tatgtattac ttaaaataac gccagcaccc
     1561 atttctttca actcttctgg ggacattgtt tttactgtag ctaatgttcc tactggcatg
     1621 aacattggtg tggggaaagt tccgtgtggc gtgatcaatt cccctaaacg cgcgccagta
     1681 tgtttttctt tcttgattaa acgatagcga atggcaggtt gcgtcattca tttccctact
     1741 ttctatataa aatatcttga caactcccac catcataatc gtttcaagtg ctttttgcaa
     1801 gttccctcgg cttccttcta ctttatttaa tattcttttt tttatccttt ttttatgaat
     1861 aacaaaaaaa agaaaaacat tatgacagaa atattttttg atacatggta tattggtgtt
     1921 attaaagaaa aggagggaaa aatcatgcaa tcttctgcag aattaaaacg ccaagcaaaa
     1981 gagagtctaa aaggacgttg gggacaagct gttttactaa atctaatccc tacattgatt
     2041 ggtattgcta ctattcttct tattatgatt cctattatct tgtttgccac caccatatat
     2101 gatggatcag cgagcgtctc agaagcgaca acttcaggag gcggtagttc aggaggcggt
     2161 ggcggaatta tttccagcat cattagcgct ttgttcatga gcggtatttc ttggacttat
     2221 cttgatattt tacgtggaca aaaagatcag atcgaaccat tcaaagatgc atttcgtgga
     2281 ttcaaaggtt tgtttatcgg cggagtgatc agcttagctg tgctgatctc gatctttaca
     2341 acattatggg gtttgctatt agttattcct ggaatcatta aaagctattc ttattctcaa
     2401 agctatttta tttactacga tatcgtccaa gaaacaggac aaacacctaa agtactggat
     2461 acgatcactg caagtcgaaa attgatggat ggttataaag gacaactttt ctggttagat
     2521 ctttcattta tcggctggca tatcttagca ttagcaactg ctggtattgg ttatctatgg
     2581 ctcagcccat acatttctgc aacaaaagca gcattttatg aacaattgcc aaaagatatt
     2641 taaaagagag tgtgacttgt gaccgagggg gtggcaggag aacaccgttt atcgagaggc
     2701 agtaaaagaa acattcagcc cattagcaaa aaggaggtgt gacaacattc atgtcacacc
     2761 tcctttttgt atagttccag taaaaagaaa atcaacttag aaacagttaa tcattcctct
     2821 tcttctgctt ccggtaatcg atggaacttg atcagttcat tcaccgcgat tggtacgata
     2881 gataaaatcc ctaccaagaa ccagtgattc aaactgatgt gtactaggcc gaatagttct
     2941 tgcgtaaatg gcagcaaagc aacagccgtt gtaatagcta atgccagtaa cgccatttct
     3001 accaaagctt tattggtcgc taaattgact ttgaagatcg attggctgct gcgtacattg
     3061 aacacatgca gaatcgatga ataagctaag atcaagaatg caacagtttg accgacatgc
     3121 tgacttgcgt ctacccatgg gctgatagta tcgacataac tacctaaata ataaccaaac
     3181 aaggttacga tggtgaagac ccctgcattt atggcaattt tttgcaataa acctcgagca
     3241 aagatccctt catttttcgg aatgggtggc tgatccatga tgccgtgttc cgctggctct
     3301 cttcctaaag caaatcctgg aagtccatca gcaactacat tgacaaacag tagttgcaca
     3361 gctgtaaatg gtgctcccca tcctaacagc atcgcaatca aaacaataaa gatctccgaa
     3421 atattacagc ttagcaaaaa gttgatagct ttacgaatat tctgataaac cgaacgtcct
     3481 tgactgacag cgtctacgat cgtcgcaaag ttgtcatccg tcaaaatcat atctgcggca
     3541 ctttgcgcca catctgttcc tgtgatcccc atggcacacc caacgttact tgctttcaac
     3601 gctggtgcat cattcacacc atccccagtc atcgctacga ctgcacccgt acgttgccaa
     3661 gatttgacga tccggatctt gtcttctggc gttacccgag catagacaga gagagctttg
     3721 acacgcgcat ctaattcttc gtctgataat tgatgcagtt ctgatcctga aagtgcttct
     3781 gacggatcat tcaagatacc taattctttc gctatggcac tggcagtcac cacatgatct
     3841 ccagtgatca tgactgtttt gatgccagcc tttttcgctc gcttgattgc tcctttactt
     3901 tctggacgtg gtggatcgat cattccgatc agaccaatca aacgtaagtc tttttccagt
     3961 gcttcagaag aaatatcttg cggttcttcg tcataagtag cataaccaac agcaatcact
     4021 ctcagtgccc gtttaccaaa gcgatcatta acgatcgccg cttgttctac atcgcctgaa
     4081 ctaaagcgcg gagcaagaac atcgaatgct cctttggtga ttgagatgta tttttcgccc
     4141 atttggtgaa ctgttgtcat catttttcgt tcagaatcaa aaggaagctc gtttactcgt
     4201 ggatattttt cttctaattc tgcctttgta tgataattct cttcaacagc ccttacgatg
     4261 gctacttctg ttggattacc tttgataaca aggtcgtcat cttcttgctc gaccgtcaca
     4321 tctgtacata gtgcagccat tttcaatatt tccatcgcgc tgttcgtcat tgaatcttcg
     4381 atattcgtta cttcatcttc tttgctccat acccggcgga cgcgcatttc attttgtgtc
     4441 agcgttcctg ttttgtctga gcagataaca ttagcggttc ctagcgtttc aactgctggc
     4501 aactgtcgga tgatcgcatg tttcctcgcc atcttctgca caccgtatgc aagtgtcaac
     4561 gtaacaataa cagtcaaggt ttccggtaca gctgcaacag ccaaggaaat cgaagtcatg
     4621 aacatctcaa ggagtggttc tccttgcagt tcaccaatca caaatactaa agccgcagcc
     4681 gctaaagcga tcatactgat tcgtttgcct agctgattca atcggacctg taaaggtgtt
     4741 ttttgcatag tattttcgtt caaaagatcc gcgatcttac ccatttctgt agccatgcca
     4801 atggcagtga cgatcgcttt cccacgacct gcagcaaccg tacatccttt aaataccata
     4861 ttaagttggt cacctaatga ctcatcttct tcagcaataa atgctgcatc tttttcaact
     4921 gcttcacttt ctccagtcaa cgccgattct tctacacgta attgtgatgc ttcgatcaaa
     4981 cgggcatcgg cagaaatagc atcaccggac tctaaaacaa gaatatcacc cactaccagt
     5041 tgctctgact caacagtggt cactttgccg tctcgtatga cagttgccgt ttgtttattc
     5101 atatcttgca aggattccag tgctttttct gcgtttcctt cttggacaat cgccaatacc
     5161 gagttgatca caacgatagc gatgatcagc agtccttcaa ataaatcccc atgttctgta
     5221 gcaaatgcgg tataaaagga aatcaccgct gctacaagaa ggatgatcgt tgtaaaatcg
     5281 gacaagcttc gaaagaattt tttgatcatc gattctttcg gcgcctcatc gaatttattc
     5341 aatccctgct tttttcgccg ttcatccact tcttgtgaag acaacccagt atctggatct
     5401 gcttctgtta ccttctttac ttcttctatt gtctgcttgt attcttccat cctattcctc
     5461 ctttaaaatc ttcgtacaaa aaaaggtact tattttacta taacatcctt tttttattta
     5521 cttcaaataa agggcttatc gattatttgt taagaaagcg taaaatttaa agtgcttata
     5581 tttgcaaacg ctaccaaagg atgctattat atagatgtaa ggaaggagga taccatcaaa
     5641 tagacagaag aatttctcga tagaacagtg taagcaaaca aagttgttag aagtacaggt
     5701 ttcacccaga catgcgtgta gagtacaaac ctataaatag tgaatgcgta tggatgtttg
     5761 gatcacgaag tatccgaaac aagaagtaat gcgatacgta acaattgaat aacgtaaacg
     5821 tctgaaaatt gaataggaac aagagatgtt tcaaatgttg aagatgtaac gaaaacaaaa
     5881 tttttgttta acatgaagaa attcaacacc gtgctttgca tgtatgatgg aaagttacaa
     5941 tcatttctgt ttatttgaat ccttccgagg tacaagaaag ccgagcttat cgagagctcg
     6001 gctttttctt tgtcataaaa tctttgtaaa gcttttttgt tattcaaaac tagcggcttt
     6061 tcataacctt atttcacgaa catcgcatct ccaaaactaa agaaacgata gcgctcttca
     6121 atcgcatgct ggtaagcaga taaagtcaat tctcttcctg caaaggcact aactaacatg
     6181 actaaagtcg attttggcaa atggaaatta gttgagaaag cttctaccac tttgaattca
     6241 tagccaggcg taatgaaaat atcagtccat ccgctgtctg ctttgatttc tccattaaat
     6301 ttcgttccaa tcgtttctaa tgttcgaatt gaagttgttc cgacagccac gattttcccg
     6361 ccatttttac gtacttggtt caatcgctca gccgcttctt ccgtcaaacg gtagaattca
     6421 ctatgcatat ggtgttcttc aattttatct acgctgacag gtcggaatgt acctaaccct
     6481 acatgtagtg tcagatagac taattcaaca ccctttgctt cgatctcatc aagtaattct
     6541 ttcgtaaaat gcaatccagc tgttggcgct gcagcagaac cattttcttt cgcatagaca
     6601 gtttgatagc gatctgggtc ttccagacgt tctttgatat atggaggcaa tggcatttct
     6661 cccaatgatt caagaatctc tagaaaaatt ccttcatact caaagtcaat gatccgtccg
     6721 ccgtgttcca attcttcttt cactactgct tgcaatctgc catcaccaaa agagattctt
     6781 gtcccaactt tggcacgttt cgcaggtttg atcaatgtct cccacgtatc tccttgtgta
     6841 ttcgttagaa gaaggacttc taaatgcgca ccagtatctg gtttttctcc atataaacga
     6901 gcaggcagaa ctcgtgtatt attcataact agcgcatcac caggctgtaa ctcttcaatg
     6961 atttcatgga aatgtttatc ttccatctct ccagttttcc gatccaaaac aagtaaacga
     7021 gaatgatcgc ggtcttttaa tggtgtttgt gcgattaatt cttccggcaa gtcaaaatca
     7081 aaatcttctg ttgttaacat atcgtcactc actttctatc atgtgcttca tcattctatc
     7141 atgtttttcg ttctgttggc aaaatcagca ctttttcact tctatttttt gttttcctaa
     7201 ataaaaaaac taccctattt gtattttttc attgttttag gcatactagt aatagatagt
     7261 tcttcattga aaatagaaaa agaggtggca ccatgattga atttcaaaat gtgtcaaaaa
     7321 tttataaagg tggaaaagtc gctgtagagg acgtcaatct ttcatttgaa aagggtgagt
     7381 tcatttgttt tatcggtacg agcggaagcg gaaaaacgac gacgatgcgg atgatcaacc
     7441 gaatgactga tccaacacaa gggaaaattt tgattgatgg aaaatccatc caagaaattg
     7501 atccagtgga attgcgacgt cagatcggct atgttatcca aagtattgga ttgatgcctc
     7561 acatgacgat ccgtgaaaat atcactcttg tccaaaaatt gttgaaagtc agtcaagaag
     7621 aacggaataa aacagctgaa aaaatgattg atcttgtgga attgccccgt gagatgttag
     7681 atcgctaccc acatgaatta tctggtggtc agcagcagcg tattggagtc gttcgtgccc
     7741 ttgcagcgaa tcaagatatc atcttgatgg acgagccatt cggtgcgctt gatccaatca
     7801 ctcgtgattc cttgcaagac cttgtcaaag atttacaaga gcgtttggga aaaacgatcg
     7861 tatttgtaac ccatgacatg gatgaagcga ttaaactagc aagccgtatc gccatcatga
     7921 gtgaaggccg tgtgatccaa tttgatacac cagaaaatat tttacgtcat cctgcaaacg
     7981 atttcgtaga agagttgatt ggagaagatc gcctactaca agccaagcca aatgcgacaa
     8041 gagtcggcga agttatgcta aatacagcaa tcacgattac accggaaaaa tcattacaag
     8101 aagcaatcaa actgatgagg gaaaaacgtg tagatacgtt gttagttacc gataattcga
     8161 atgtattaaa aggatttatc gatgtagaaa cgttgaataa aaaacgagga aaagtatcca
     8221 gtgtcggcga cattttgaat aaacatgttt tttacgtaaa agaatccgcc tatcttcgtg
     8281 ataccttgca acgcatttta aaacgcgggc tgaaatacgt gccggtcgtt gatgaacaga
     8341 acaaagtcgt aggtatcctg actcgtgcat cattagtgga tatcgtctac gatgtgattt
     8401 ggggagaaga tcccgcaact actgatgttg cagagtcagc tactcctgaa gaatctaagg
     8461 aggtctgacc tatgcagcag ttttttcatc agcacggttc agaaattctc tcaaaaggtt
     8521 ttgaacatct ttatatttca ggacttgctt tgatcattgg tatcatcatt gctgtaccgc
     8581 ttggtgtctt actgacacgt tttccaaaag ctgccacgat tgtgattggc ttaaccagtg
     8641 ccttgcagac aatcccttct cttgctttat tggcattgat gatcccgcta tttggcgtag
     8701 gtaaattacc tgcgattatc gcactcttta tttattcatt attgccgatt ttacgtaata
     8761 cgtacattgg gatgaaaaat gtcgattgga attatcggga tgtggcaaaa ggcatgggga
     8821 tgactgagtt ccaatccatt ttttccgttg aattgcccat tgctatgcca acgatcatgg
     8881 ctggtattcg tttagctgcc gtttacgtga ttgcatgggc tacacttgcc tcttatatcg
     8941 gcgctggtgg tttaggcgat ttgattttta gtggactgaa taattaccaa ccagatctca
     9001 ttttggcagg tacgatccct gtaacaattt tagcactatt ggctgattgg cttctaggtc
     9061 tcttggaaca ccggctgaca ccgatcgcac ttagagagga gaatgaagaa tgaaacgtat
     9121 actaaaaatg accatggttc ttttctctct tcttatactt gcgagttgtt ccttccctgg
     9181 tcttgccagc aatacagatg aagacaccat ttcgatcaca ggtggaatca cttcagaagc
     9241 acagatctta gcaagtctag ttgccggaat ggttgaacat tatacagata aaaataccgc
     9301 aatcatcaac aatctggcta ctacaactat caaccatcaa gcgatgatga acggagatgc
     9361 ttcaatttca gcggctcgct acacaggaac agatttaacg actacattga accttcctcc
     9421 tgaaaaagat ccgaaaaaag cgtttgctac cgtgaaagat gaatttgaga aaagatacgg
     9481 tcaaacgtgg ttcccgtcct atagttttga aaatacgtat gtctttcttg ttcgaaaaga
     9541 caccgcacaa aaataccatc tatctaaagt cagcgatctg aaaaatgtag cggatgaatt
     9601 agtagctggt gtagatactt cttggatcaa tcgtaaagga gacggttatg acggcttcca
     9661 agaaacttat ggcttctcct tcaattcgat attacctatg cagattggac ttgtttatga
     9721 cgcagtcgaa gcaggaaaaa tggatattgt tcttggctac tctacggatg gacggatcgc
     9781 cagttatgat ctagtcatgt tggaagatga tcgtcaattc ttcccaccat atgatgctgc
     9841 tccagttgta gacaacaaga tattagctga tacgcctcat ttaaaagaag ccttaaataa
     9901 gttaggtcat accatcagta cggaaaaaat gcaggaattg aactacgaag cagataataa
     9961 tctagtggaa ccatcagtag tagcacaacg tttcttacaa gaaaaccact attttgaagg
    10021 gaagtgaagc aattggataa aatgaatgtc tttcaacaat ttttctatta tttccaggaa
    10081 aatggctcct atatctttgc acaatttatc cgtcacttcc tgatttctat ctatggtgtc
    10141 ctctttgcag cagttgtcgg gattccggtt gggatcatga tttctaggag acgtaaatta
    10201 gcaaactggg tgattcgttt ggcgaatatc atccagacga tcccttctct agccatgatc
    10261 tccattttaa tcattggatt aggcctggga gtcaatgtcg tgattgttac tgtatttttg
    10321 tattccctct taccgatcat caagaatacg tataccggga tgatccaagt agacaaaaac
    10381 atccttgatg tcggcaaagg catggggatg actgctagac aacgactatt catggtagag
    10441 ttaccactct ctgtttctgt tatcatggca ggcataagaa acgcgttagt tgttgccatc
    10501 gggataaccg cgatcggtgc attcgtcggt gctggtggtc ttggagatat cattatccga
    10561 ggaaccaatg caacagaggg cacatcgatt attttagcag gtgcattgcc aactgcatta
    10621 atggcgatca tcacggactg gcttttaggt attttggaaa gacggctaga tccagccagc
    10681 cgtacatcac gctgatgtta ttgtaaaaaa caaaagcttc tgcgaccatt tgttggtcgc
    10741 aaaggctttt ttagttctgt catttttaag gcaataatcc actctgattc ttccctcctt
    10801 atgggcaaag agttgctttt cttagctatt tatccattcc gtatactcca acttcatact
    10861 gccaacgggt tcatatctgc atccaccgac taagtttttt tcttatccgc taaggcattt
    10921 tgccaatcca ttttcccatc actcgtcatg gaattcatac cacatgatct ctaaatactc
    10981 gtctgcttgt tctttgaatt gggcatcaat tcttacatcc tttggcgtgg ctttcattta
    11041 agccttttcc atcagtcctc gattgttcga tgccttcagc tgcatcatca aaactggtga
    11101 ctgccgtatc ggtaacttcg atcatctttt atacaatcgg gaacttgcca tcctcatcct
    11161 tggctaaaaa catatctagc cattccagct tctcgccatg ggaggtttgc ggttcctaaa
    11221 accggtacac gattcttatg gactgcatcc gtcgcaacta gacttggcga tacgatgatc
    11281 cttcaccaga agagcacccc cgtaaaccaa ttgatcgata tattgccaat aagaaaagac
    11341 attggcgtaa aagatatcga tccctcgtga tgcattgccg ttgatactgc tattcatggc
    11401 cgaaagcgcc acgactttaa cttcttccgt ttgagtgaca ttcatgttcg gcaatgtcgc
    11461 tttggatacc cgctctgcta aaggaacgac actggtattg tatttcgtat ctgtatcttt
    11521 tccaaaagac catgccaacg aatcctctgg gaaccaataa gacgactccg gttgattgtc
    11581 cattgctttt ttcaacatac ttttggctgt ttctcgataa acgacatttc caattgtctt
    11641 cgcctcactt tggcgataat cgatggtcgt tgccactgcc aacactaaca acgatgctcc
    11701 aattttactg attttccttg tcacacttct tcctccttgt tttacataaa cccgtcaaca
    11761 ttccattcct aaactcaaac aatgaccctt caaaggtgga gatataggaa caaattaaag
    11821 aaatcatgca ataaatccaa gaaggttggt tttagaacaa aaaaagagtc tcctttttgg
    11881 ggagactctt tgttaactat ctaaaaaggt tggctcttct cacttcactc ataatgtgtc
    11941 caggcagaat aaatttctac aactttattt tcctcatcaa ctttgtaaac cactcgatgc
    12001 tgtctattca gcctgcgtga gtatctgcct tcatgtgtag gtctcagctt ttcaaaagac
    12061 tgcgtgggca tatatggatc ttccttcaat gtctgaatca cttcttcaaa ttgtttcttt
    12121 aaattcgttt gcttgatttt tcttaaatcc actttcgcag agtttttgat tgcgactgta
    12181 tagttactca tcaaagagct tcccaatcaa tatcatcgat attcgtaaag ctactgtcgt
    12241 ctttttcgcg gtctcgtact ttatctaaag tacctgtttg ctcaagaaaa agggtttctt
    12301 gaatcgctct ccagtcttcc agcccaatca atacagcatt gtttccacta cgatcactaa
    12361 taatctcgat ttcagtatga ttttcattta cctctttcaa taactgataa aaatttttac
    12421 gagcagtgct aggattcatc gttgtattag ccattgtctc tcctcctttt tctgatataa
    12481 ttatagcgta cttgattaag tacgtcaaaa aaatacaata aagcacttaa attcagcaaa
    12541 cttttcctct tttttacata gagaaaaaga ccccttttta gggaggatct ttgtccacgc
    12601 tcacttctta ctcctttacg actagcggta gccaaacgta aagtaattgg taacaacgta
    12661 tcatctttag caaaggtcga atccaaagcc tgtgagtcta tgcaacaaca ccaatacacg
    12721 aaggttcctc gttgcagtgt gacttgattg atatcttgaa taacctgagg atacgcacga
    12781 aagatttgta acggttattc aaaactgatt ttggccgtca agtcttctac tgcctcaaaa
    12841 atgattgcct ctttcgtctc ctgtcattca tgttcactat cgatttgata cttgcttaga
    12901 ccgttgggtt tgtgaatcac caattaaaaa tggcactgag ttatggtaac aaaaaatggt
    12961 cacttgacga ggctgagatt cctacttatg aagacacatg gcacttaaga gtcgaggtaa
    13021 aggaagaagc tgaaaatata ggaggctgta cattatttgg caaaggattc ggaaattatg
    13081 atcaagacat caaaatcaaa atgttttaca gctaaatagg aagaataata acatttcata
    13141 cgctgttccc atctttaatg gcttatagag ggcaccttta gctgagaata agacaaaaca
    13201 gaccctctag cttcaattaa gagctagagg gtctgttttt gattttaaaa cttggtttat
    13261 ttcttcaaat ggtagctata ggtgctgaca acgatatttt cacgccaaga caatcgcaat
    13321 cttaaccgca aacttgtttg gttatggaga atattctgcc agcgtctgtt tggtacaaat
    13381 tgaggaatca acacagtagt tgtatagttt tgtttcgctg catttttact tacgatatct
    13441 acataacgga taattggatt ttcaatcgag cgataagaag agtggacgat tgaaaaacgg
    13501 acatctggga aatgcttttt aaattctgct tcgatttctt tttctttttc gacatcttcg
    13561 tctagcgaaa catgcatcgc aacgacataa tcaccgatcg aacgtgcgta attcaacgca
    13621 ccgatattta cccgtgtcac attcccgact aatacaagaa cggtattccc gtcataatca
    13681 tgtagttttg catcattttc taaacgaagc tgttctgcta cttttttata atgatcatgg
    13741 atcttataaa aaatgaacat aacgattggc ataatgatga agaaaggcca aatgtcccct
    13801 aaccgataga caaacaaaat cgcaatgatt gcatatgaga tgaacgcacc cgtaatattg
    13861 gcaatcgatt tgcttaacca tctttttcct tcttttttcc atttgacgac catccctgtt
    13921 tgagataaag caaatggaat gaacacgccg atagagtata acggaatcaa gcgttctgtt
    13981 gagccatgga aaataaacag caatacgatc gaacctagcg ctagtgtgat aatcccattg
    14041 gaatagccta gtcgatcccc acgatcttga tacatgtgag gcatgaattt atcttttgcc
    14101 agattgtatg caagaactgg aaaagcagaa aaaccggtgt tagcagcaac tgctaaaatc
    14161 agcgctgtag caaattgaag gacgtaatac aaaataccat gtccaaagac agctttacca
    14221 atttgagaca aaacagtgac ttctttttca ggaacgatcc cataccaata gttgataaat
    14281 gtgatcccca cgaagaagaa acctaagatc aatgccatca tcgtcaatgt agcagcggca
    14341 ttttttgctc ttggcttctt aaagaatggt acagcattac taatcgcctc caccccagtt
    14401 aaagaggaag aaccagaaga aaatgctcgt aaaatcaaag cgatagaaac acccggcaca
    14461 gccgctcctg gaagtgcagt agcttgcaat ggcacagcac ctgtcacgat attatataaa
    14521 ccaacaacga tcagtaacga gatcacaagg ataaatgtgt acaccggaaa taataggaac
    14581 gatgcagatt ccctcagccc acgaagattc agcatcatca gtagcaaaac gattgtcaca
    14641 gaaatagcga cttgatgacc gtagagtgct ggcaccgctg atgtgatcgc ttcagcacct
    14701 gctgagacag atactgctac tgtcaacatg tagtcgatca ataaagagcc accggaaatc
    14761 aagccagcat tctttccaag attctcactg ctgacgacat atgctccccc gccatgagga
    14821 taagcatgga tgatttgacg atatgatagc gttaatgaaa tcaataaaat aataacaaaa
    14881 gcggcaatgg gtagcgaata ccagatggct gcagcagaca aagcgactaa aacgacaacg
    14941 atttgttccg taccatatgc aatagatgat aacgcatcag acgacaataa tgccaaagca
    15001 gcaaatcggg ttaatttgtg ctcatcattt tcagcagatt ttaaaggctt acctactaac
    15061 aaccttttta agtaatccaa tttctaaact cctttacaca caattttact tcaagtttta
    15121 tagtcgaaaa tttatagtaa acaagatgtt atagtacgct cactgacctt aaaagtcaat
    15181 aaactttaag aaaataattg gaaaaaaaca gaaaaatatc aagcgctttt tcaaacgctt
    15241 taatacagca aaaatctaac atttttttat gggctttttc tttgaattct ataataaaag
    15301 gatagaactt gacttagaaa atcaagttct atcctttttg atatcttaac ttttggaaga
    15361 gagatgtaag gctgctttct acccatcatt cttactactc gaagcatagc gcagttgtca
    15421 caacctcttg ataaacggtg tttaaaagct tgcatctgcg gtaataagcc caaccaaatt
    15481 aaaaacataa gaagctgttt ttatttggtc gttcttatta cttgatgcag agcagctttt
    15541 ttacaacctc ttggtacacg gtgttcaatc agctgaccct cggtattagc tcaaaaaaac
    15601 ggcaaaatat gaggaactat ttttccattt ttttctctaa tactcaggtc aaaacactga
    15661 tttcacaacc tcttttttta atgaaaaata tggctctcat gcgccgcttc tttgaatttc
    15721 gtcatttttt cttcgatgaa cggatagatt gccgttccta cgattccaaa gacaacaaac
    15781 aaaccgatca tgatccagat tgctttcgta gcatttggcc aataaattcc tcctgcagat
    15841 tcccgtaaca agtttaccgc atgagtaaat ggcaagaacg gattgatcat ttggaagaat
    15901 ttccctgaga cttggatcgg atagttcccg cctccgccag agatcgacag tactaagatg
    15961 ataatagcga tccctttacc gacattatcg aataatgcag ccaagacata gacgatcatc
    16021 atgaacgcta aggcgatcaa gacagcaaat gacacgctgt agaccggatt ttggacatct
    16081 actcctagta agaaaatatt ccctaatgta acgatcaagc tttgcgcaat cgccattgtc
    16141 aaaaatgtca gcatacgagc aacgaattgt tctcgtttcg taaaattgtt tcggtctttc
    16201 tttcctaaat agtattctgt cgtagcaaca cttgaaagaa gaacagcgcc gacccacaga
    16261 catagtgcag tataaaacgg ggtgctagct gaaccgttat ttgcgattgg ataaagcgca
    16321 ttggtttgta gctcaacagg ttgtgtaaag aagtcacttt cttttgttgc gtctagtttc
    16381 aataatttga tgacttcgcc tagatcgact tcttgttcgc cttttcggat cgcgtctgct
    16441 gctttttgga taccagcttt gatatttggc cagtcattgt tgatcaagtc cgcagcagcg
    16501 ttcacagctg tttcgacttc tggcattttt tcatttactg ttgtcaatgt accggtgatt
    16561 tcatttttta gtgctggcca atcattttgg ataaaatcag cggccaatcc taatttttgc
    16621 tgaatgatcg gtaattcatt gttgtatagg tctgcccctt cattgatgcc attgacgatg
    16681 gtatccatat ttccattcag cattgtattg gcatcatgga tttcttgtcc aattgcaggc
    16741 atttcttttt gatatttttc taaaattgcc actgcattcg ttacagttgc tttcgttgag
    16801 ctgagaagcg aatcaaagtc gatctggtcc gctttgctca gtgcgccttg tgcagtccca
    16861 atcgtatcga tgacttggtc tagtagagaa gtgacagtgg aactgatttg acctgtattg
    16921 ataccagcaa tgatgccatt gatattatta acaacggcat caatttgatc tagcaattca
    16981 tcaatagcct tcgtgtcttc attttgaaca gctgtgttca aagcagatag gcggttttgc
    17041 agattgctta gcaagggttt taattgattt agctgatcaa taattggctg taatttcgta
    17101 tttccagcac ttgtttgcaa tttagtcagg aactcgacca gtttatcgat tgcattttgt
    17161 tgttgagata ttccgtcaat aaagccgttc aatacattgc tgatgttttt cttttcatca
    17221 tcagtcagct taccatcttc aatagctgtt cttacagtat ctattaacga acctgtcgtt
    17281 gttgccactt gtccaattga cttcaatgta acatctacac tgtttgtgat gcttggtatc
    17341 gcatctttca gctgagaggc acctgatttt gtttggtttg ctagatcact tgcctgattt
    17401 tctaaatttt tcacatctgg caagatatct tgtacttgtt ggatgatcgt caagccttgt
    17461 tttgcttcat cgattccttg gttcattgtc tcttggatcg aagcaaaatc gccgtcgatc
    17521 tctgctagtt gcttaccggc attttggatc tctgggattt tttcttgcaa atcaaggatg
    17581 atttttgctt gatctttcaa ctcaggcatt ttgtcattca atgcaacgac tttttcaccc
    17641 atctcatcta ctttagggat gtaatccacg aattcatttg ctttattcag tttttccttg
    17701 atttcaggta gttgactttg taattcgagg acttgttttg tgtagccgtc gatcgtgtcc
    17761 aaattttcat ctgttgataa aatcatatct ttaactttat tgatacttac taggttcgta
    17821 tcgatgtcgt aacctatctc attgaatact ttcaataaag tcgaactggc tgttttgata
    17881 aattcatttg tgatttgcga ttggatcgaa cttgctcctt tatcggtgat tttcggtgca
    17941 atcgcattga ttttttcatt taccgtatat tcgatttttg gtttcttgat atcaccacta
    18001 gtaaaactca gcagatcttc tgagaaatcc tttggcaagt aaatacctgc gtaatacttt
    18061 ccggaacgga ctccgtcttc taattgttct tttgaatcaa cgaattgcca tcctaattgt
    18121 ttatttttat gaagcgattc gatgacttgc tccccaatgg ccacttcttt accttgaaac
    18181 tctgctggct tgtctgcact gtaaacagca attggcagct ctccagtatt tccatatgga
    18241 tcccataatg ctttgatgtt aaaccacgca tatagcgaag ggatgatcat gatcgcgatg
    18301 atcagaagtg ttgcaatcgg gtttttgaaa atccgtttcc agtcaagcaa atataatcga
    18361 agcgtatttt tgattgactg catagttctc ccccttcttc ttttttcagc tgaattactt
    18421 taccatgtat ttcatacttt tttcaatcgg cttttttcaa aaaagtcgaa tttttggaca
    18481 tatcacggca attgtctcat tgtaatctct tacatattga gaaataatta aggattacag
    18541 cctttacttt atcatgaaat aaaagggaaa gataatttta tgccaaagtt tttatctatt
    18601 atacgtatac gcaaaaaagc taaaacattt tttgtctttg cccctacaaa aatgtttcag
    18661 cttttttcgt tttttattcc tgtatttctt atttttcgaa tgacaggatg attttcccag
    18721 caggaggatg tccttcaact agctgatgtg cttggatgac atgcttaagt gaagcagaca
    18781 gattcattga aataaaaaca tgaaaggctc ctttttggta ggctttgatt atgccagcaa
    18841 aggcttctgc atctagatat tcctttcttg gaacaaacga ttcataaaag ccttcctttt
    18901 tttgccgcaa atctagatct gggaggtcat ttaatgcaac atacctgccg cctggtttca
    18961 tgatctggat gccggtttct cctttgatac ttcctttcgt cgcatcgatt acaaggtctg
    19021 cctgatcagc aaattgcaga cctggatttg ttttatcata agcagcaaat gctgaagctc
    19081 ctaatttttt taccttttcc tcatttttac tggatgcact ggttaagatt cggattccct
    19141 tttcatggag aagctggatc agacttgacc cgaccgcacc tgatgctcct agtatcatta
    19201 cagtatctgt cggctggatt tccgtcaaat ggttgattag attataagcc gtaattcctg
    19261 gcgtgaccat tccggctgct tcttcccaac tcattttatc tggaattttt gctacttttg
    19321 ctgatggcaa cacgacttct tctccgtaag tcccgccgac tgcatggaca gctactcgat
    19381 cacctacatg aacagttgtg acatctgaac caatctctgt cacaatacca gcgccatcat
    19441 tacctggtac ataagggaat ttcaatgttc gtatctcttt catttctcct aaacgcaagg
    19501 caacatcata aggattcaca ctgaatgctt ttatttctac acgaacatgt ccagaaccga
    19561 cttctctggg atgagcatcg atttcttcaa aaacatcttg tgcttctccg taaccgttaa
    19621 ttgcaaatct tcgcatggtc accatctccc tttttcttta agtttacgtt tatgccttta
    19681 ttttgacaaa aaaaatgtct tttcgttttg gcgcatgatt caaaacaaaa tagttgtctt
    19741 ttttcgcgta acgtgttaaa ttatttatct aaaagcgtaa agattacgat ttacgctaat
    19801 aaaaaataat tggaggaatt tgttgatggc taaaaaatca aagattgcca aagcaaaaaa
    19861 acagcaagaa acgattgaac gctatgctgc tttacgccag gaattaaaag cgcaaaaaga
    19921 ttacgaaggc ttgcgtaaac ttcctttaga tgctcgtcca gaacgattga aaaatcgtga
    19981 tttaatcgat ggacgccctc gcggatacat gcgtaaattc gggatgtccc gtataaaatt
    20041 ccgtgaatta gcacataaag gtttgattcc aggagtaaaa aaagccagct ggtaatccaa
    20101 gtctggctgt aatgtaaagg aggagggcgc atggaccact ccttggatca tcaaaaaaga
    20161 tacgttcgct tcttctagca gaaatgctgg tactagaagc agacgtatct ttttttacgc
    20221 acagctggtc cttttttata tgtaaaggct gcatggttac ttcgctatat ttatttagta
    20281 acaggagtca gtgcttcgat acccacacta attttcaatg ctcggtcaac ttctgccata
    20341 atgggtcgat ccaaatggca gaccttttct ttcaatcttg atttatcgat cgttctgatc
    20401 tgctccaaca ggataacgga gtctctttct attcctgttc cagtagcttt gatcccaatg
    20461 tgagtcggaa gcttaggttt agccattttt gccgttatgg ccgcaacaat gatcgtcggg
    20521 ctaaaatggt tacctagatt attttgaata accaaaactg ggcgtgttcc gccttgctcc
    20581 gatcccacga ctggagaaag atcagcaaaa taaatatctc ctctttttac cataggatcc
    20641 tccttattct tgatattctc ttggtatccg ctgtcctaat ccgcaagcca cttcatagtg
    20701 gatcgtacct aattgatctg ctatatcctg catagtgatt tctttgtctg catttttgcc
    20761 gataagcgtc acttttgttc cgacaggaaa ttcttgaggc aatcggatca tcagttgatc
    20821 catacataca cgcccaatcg tttcacagta gttcccttct accaatagag aaaaaccctg
    20881 catctttcgt ggccagccat ctgcataacc gatcggtatc gttccgatcc actctgcttc
    20941 aggagtgata tatgtttctc catagccgat tccttcacct gctgggagtt ttttgacttg
    21001 aatcaattca gaaaccagct caagtgctgg ctgcaatgga taaacttccg gtagtgcgtg
    21061 cccagaggga ttaagtccgt acattgctac tccataacga atcatattgc cgattgtttc
    21121 atcatgccat aaagccgttg cactattgct gacatgtaca tatcgaggaa gttcgggaag
    21181 ttttttcagc acttctataa agcgttcttt ttgcagattc caatatgtat catctgcctg
    21241 atctgctgta gaaaaatggg taaataaacc ttcccattct aatacacgac tttctttgat
    21301 gaatgcagct gcctctttta cttcttcagg tgtacaaaat ccgatacgac ccatcccagt
    21361 gtctgctttt agatgaatcg aaagaggcgt ctctagctta cttttctcta aaacagcagc
    21421 tgctgcttgc aaccattccc gtgtacctgc agttactgat aaatcatgag caacagctac
    21481 agtcagtgcg tcgtaaggaa ccatattcaa gataaggatg ggctgagtaa atccagcttc
    21541 tctcaactcg actccttcat ctaaatttga aacacaaaag cccgaagctc cacctgcaac
    21601 tgctgcctca gctgtttcaa tagctccatg accgtatccg ttcgctttta ctactgcaaa
    21661 caattctttt ccttgcggaa gtcttgcaac ttcatttgct acattttcag ttatagcttt
    21721 tttatgtatc actgctttcg ttgggcggtg ccaagcgaca accatcgttt ttccttcttt
    21781 ctagtcttcc aaaataattt gtgcaaaagc tgtttcattt gtatgagtaa tactgaccca
    21841 tacattgcct tcatgaggtg aacgcgtcac aacaggctga ccattttcat tttttaaaat
    21901 ctcgacatct tgaaaggtaa cttttccgat cccagttccc caagctttag agaaagcctc
    21961 tttgcaagcg taacgcccgc ctaaaaattc tacttggcga tgaaaaggca aggactggaa
    22021 tagtttcatt tcatcagagg tcaagattcg tgcaatgaat tttggttttt cttcgatcag
    22081 ccgagtgatc ctaggaagtt cgactgcatc gatcccaatt ccttttatca tatgcttttc
    22141 tcttttcaat aaatttagaa cactctcatt ctaccaaatt ataaccattt tgccacaaat
    22201 aagtccacaa ataacaaaaa tgaaagtttc tcttaaaaaa gagaaacttt cattttatgt
    22261 ttaatcgtta tttgaacgga ttacaaatcc gcgtttttta tcattgtgac gtttaccgcc
    22321 atctttttgg ctgttattat ttttgctata accgcctttt gatttgtttc cacgataatt
    22381 accgccgtca cgacgattac ggttgtttcc gccaccacgt ttgccatttt tattgtatcc
    22441 ttttttctgc attggcaatg ggcgttctgg tgtgatcttc actggcactg catcagctgg
    22501 atcttttgct gtcgttttta gcaaaagtgc aactaaatct tgtgcagaat attcttctaa
    22561 taatttatct gctgtttgca ggtatttatc tagaccattt tctgcaagtt ttgtttcgat
    22621 ttgttcaact gcagcaccta attgaccttt gaatgcttct ttttcggttg gtggacgtaa
    22681 agtcgtcatc cgttttttcg tcaagttttc aatcacatgc aagtagtcca tttcatttgg
    22741 tgtaacgaaa gtcacagaca taccgccttt tccggcacgg cctgtacgtc cgatacggtg
    22801 aacgtagctt tctggatctt gagggatatc atagttgtac acatgagtga cacctgaaat
    22861 atctaatcca cgtgcagcaa cgtctgtcgc tacaaggata tctaaatgac cgctcttaaa
    22921 tgaacgcaat acgctcattc gtttttgttg agacaaatcg ccatggattc cttctgcacg
    22981 gtaaccgcgt gcttctaatc cgcgtgccaa ttcgtctaca cggcgttttg tacgtccgaa
    23041 gacgattgtt aattcaggtg tttgaacatc aaacaagcga gtcatgatat cgaatttttc
    23101 gtattctttt gcgcggacat agtattgatc gatcaagtca gctgtcattt ctttcgcttt
    23161 gatcttcaca tgatgtggat ttttcatgaa tttaacgcca atattcttga tagctggcgg
    23221 catcgttgct gagaaaagta atgtttgtct ttgatcagga acttgtgaaa tgattttttc
    23281 gatatcttcc aagaagccca tattcaacat ttcatctgct tcatccaaaa ctaatgtttg
    23341 aacggttcca agtttcaatg tgtggcggtt aatgtggtca agcatacgtc ctggtgtacc
    23401 cacaacgata tgtggacgat ctttcaagcc acggatctga cggccaatat ctgctccgcc
    23461 gtaaacggct tgtacacgta cttttttgtc gcgccctaaa cgatataatt cttcttgtgt
    23521 ttggatagct aattcacgtg ttggtgcgat aactaaacct tgtaattcgt gacgatctgg
    23581 atcgattttt tctaacattg gtaatccaaa tgcagctgtt ttccctgtac ctgtctgtgc
    23641 ttgtccaata acgtcttttc cagctaatgc tagtggaatc gtttctgctt ggataggcgt
    23701 tgcttcttca aaaccagcgc gttcgactga tttcaacaat tctggtgata attctagttc
    23761 tttaaatttc aaatgaatcc tcctatatgg ttgttaactg aggatgtagc ttttttgcta
    23821 ccccgtcgat ttgttcggtc aaagtgtatt gtttcttttt gacagtggcc acgcacgttt
    23881 ttatccattc ttagcccctg tggcaccacc gaaaaatcac tttgcgaagt ttaggcatta
    23941 gaaaagccaa acgggtcgcg ttcagcttta tcattattat gacatctggc ttcaacaagt
    24001 tgtttctatg gcaaaaccag tccaatttct cttcatcttt cggcaatagg ttattctaac
    24061 atagtttttc gttttctgca agtttacaga tttttatttc atttgctgaa gttgttctac
    24121 cacttcaaat aagcccatgc tgttgctgcc ttttagcata atactatctg ttgcttcaag
    24181 attttgtttg atagcatcaa tcaatttatt cttttctttt tcaaaataga acacaggtag
    24241 ttgggggtat ttttggttca attcttcttt tagtgcatgc atttgttccc cataaaggaa
    24301 aatcatggcg aatttctcag gatcgatatg ttcactcatt tgcgcatgca tatttaaaga
    24361 ctctttccct aattccagca tatctgctaa cacagcgatt ctgcgtcctt ttgttggcag
    24421 atttttaaaa gtatctaata ccagtcccat ggctgttgga tttgcattgt agacatcgct
    24481 caaaatagct gcaccatttc ctgccgttag ccattcggtt ctgttttgag tgatagaaac
    24541 atgggcgagc ccttggcgta tctcaggtat ggacagtcca aagaagcgcc cgatcgtata
    24601 cgcaatcaaa gcattcgtta cattataact tccaggcagc gggatcgtaa atgtttctcc
    24661 ttctactaca aaatcggttt gtgcttttcc ttttgctgtc acttccgcat gaacatcgcc
    24721 atttccaata ccgaatgttg tgactgtttg tactaatgtt gccgttaaag gtgtcagcaa
    24781 cggttcatct gcgggcacaa cgagtaatcc gtctgataca agtccgtcag tgatttccat
    24841 tttggcttta gcgattcctt ctcgcgaccc aagattttct atgtgtgctt caccgatcat
    24901 tgtgattgct gcagcttttg gttgagccat aagtgaaagt tccgtgattt cattggcgtg
    24961 atccatgccc atttccaaaa cgatcttttc tgtatcttgg ggcatgtgca aaatcgtgta
    25021 aggaagacca atattattgt tgtaatttcc ttgcgtctta tatgttttgt atttttcaga
    25081 caagacagac gcggttagat ctttcgtggt ggttttacca ttgcttcctg taattgcaac
    25141 gacttctgcg cctacttttt ccaagtaata ggttgccaat tgctgcatgg ctttcaaagg
    25201 atctgctaca ggaataactg gaaaattttt tggagcttgt tcaacaggca agctccaaaa
    25261 tgaagcagat gctccgtttg tgatcgcttg ctggataaat tcatgtccat ctcgcgtacc
    25321 agcaagagga acgaataaac ttccttcggt tatcttccgg ctgtcaaatt cgacattggt
    25381 aatctttgtc agtgcagagc attcgctgcc cacggcttgt gcaatttctt gtattgttag
    25441 attcattgag atctctccta aaaaaggttg tgaaaaaagc gtttggccta aacggcgggt
    25501 tcgcatgact ctcacttcca agtaaatttc tctattcgta tttagaagct agaatgaacg
    25561 cggttacgca atcactccag cctcttcttt atttctcttt attcattttt ttcaaaaaaa
    25621 gattgacgct gatggtatcg attcattcct aactggatca gttcttcaat caaatcaccg
    25681 tatttcaagc ccatattttc ccataagagt gggtacatac tgaactccgt aaatcctggc
    25741 atagagttta attcattcag gaataattca tttttatttg tcaaaaagaa atcgcaccgg
    25801 ctcaatccgc ttccacctaa catcgtgtaa gctaacttcg cgtactcttg cgctttttga
    25861 taaacttctt ctggcacttc ggctggaatc tgcatttcga ttttattatt gatatatttt
    25921 gcttcataat catagaatgc tacgtctttt acgacttcac caggcaaagt cgtccgaacg
    25981 tcttcatttc ctaatacagc aacttcgatt tcgcgcgctt caattccttg ttcaacgatt
    26041 gctcgagaat catactgata ggctgttgct aaagcatttt gcagctcttc tcggttttct
    26101 gcttttgtaa tgccgacact agaacccata ttcgccggtt tgacaaacat cggataaagc
    26161 aaagaacctt cacattgatc aaatactttt ttaggatttt ctttccattg attcttaagt
    26221 actggtacat aaggaacttg cggcacacca gcagcttgta aaatatactt ggtcatgatt
    26281 ttatccatgg cacatgcact ggtcaatacg cctgcgccga cataaggcat attcaatgtc
    26341 tctaagaagc cttggatcgt tccatcttcc ccgtttggcc catgtaaaac tggaaaaacg
    26401 atggctcctt cttctttgat ttcgcccgga ttgatcactt ttcctgtaaa gccttcctct
    26461 gtctgtccac ttgggtccca tgaaagatgc aagacatctt tgctggcagg tttttctgtt
    26521 agtaatggac ctttgaccca ttgtccttct tttgtaataa atacgagttg aacttggtaa
    26581 taattataat aaatggcatt taaaactgaa aatgcggaaa gaatggacac ttcatgctct
    26641 gcgctgcgtc cgccatatag taaagtaatc ttcaaaagat tgtcctcctg tattctaaat
    26701 cgttcagtga tttactctca tattttagca caaaacaaaa ataaacgaat aacttattta
    26761 taaaaaaacg agccagcttc taaaagaacg agcgtcattt attttttgtc agctagatta
    26821 tttgtcccgt tttttataaa aagttatgat aaagaaaagg agggatgatg atggcgcaaa
    26881 caaatccacg cagccgcttg gcagaaatca ttcgagtgtt catcaattac aatgtagtgc
    26941 caaactttgt acagcaaaaa aatcccgaac aggtaaaaaa agcctttgaa gaactaggcc
    27001 ccaccttcat caagatagga cagatgcttt ctgttcgaga agatttgctt tcttctgcct
    27061 tcactcaaac attcaaaaca ttacaagata gtgtacctag cgacacattt tcaacggtca
    27121 aaaagacgat cgaaacagaa ctttctcttt cattatcaga tatattcgat gatttttcaa
    27181 aatcgccttt tgcctctgct tctatggggc aggctcatcg tgccaagctg aagaacggtg
    27241 acgcggtagt cgttaagatc caacacccta acatcgccga agagatccga cttgatcttc
    27301 aactatttga acgagcgatc cctttgatca aatacattcc tgaaactagc gtagttgatc
    27361 taaaaggggt actccaagaa gtaaaacgtt ccttgatcaa tgaaatggac ttcttaaaag
    27421 aaagtcaaaa tggcgaacaa ttctatcaaa agaacaatgg ctggaaagag atccgcagcc
    27481 ccaagattta tgacgctttt tgttcgaaaa aagtgatcgt gatggaagaa atgtctggta
    27541 aaaacttgaa tcatttaatg aacgccgaaa acaaaactga aacttttatc actggaattc
    27601 aaaacaagca gctgaaacaa gaagttgcaa agcttttagt tgagaacttt atgaaacagg
    27661 tgtttgatga tggttttttc catgctgatc ctcatccagg gaatctgcta ttccatgtac
    27721 taacaaaaga agaacagaca caagctagcc gaaaaactga aacagtccat gaaaaagagt
    27781 tcggttcctt tgcttttcga gcctccactt ctgcagaaga ccctgttgcg ccatatacca
    27841 tcaactacat cgattttggt atgatggggc atctttctgc aggacttagg caaaaactca
    27901 cacaagccgt tttagcgctg tatacgaaag atgcttatcg aatcgaaaaa gcggtccttc
    27961 gactttgtca gcaagaaggc tcgtttgatg aaagtcggtt ccaccaagaa ctgacaagtt
    28021 ttcttgaaca gtattatgat tctcctattg atgaaatcaa cttgcaagaa gtattcacac
    28081 atgtcgtgac gatttgtcat caaaataatc tgcagttcga tcgtgatatc acgcttttgc
    28141 tgaaagcatt tgggacatta gaaggggtga tccgcgtcct cgatcctgaa gtttccctca
    28201 tggaagtcgc ttcgcctttt gctcaacatt attttctcac tcatctggat gtagaagata
    28261 cattgaaaca aagcggatta gacttattgg aagggatgaa ggctgcgcct aagatccctc
    28321 aacagctcca ccatctatta gaaatgtgga cttcgggaca aggaaaagtc aatttggaat
    28381 tgaaaaagca agacaaatta ttgagcagga tcgaaagtat gatcaatcgg cttgttttcg
    28441 gtatgatttt agctgcattg atcgtagggt cctccctgct tgtccaagca gcacctgtag
    28501 aaaacgcaga ggtcgtttcc ttattaggga tatttacgta tgctattgcc gcattcgtca
    28561 ttattttttt ggcaatcgat gcattgatcc aaatgtataa gaagaggaaa aagtagaaaa
    28621 aggttgtgac agaaagaatc agcttcaaga aataagaagg ggttttacat aaattgtttc
    28681 tcaaattttt gtgaaaattc ggcttatttt tcgaagaggc tacttctgaa acaccgagaa
    28741 taaaaaatgt gaaaaagctg ttctgcaccc agtaataaga acaatcaaaa agggaacatg
    28801 gaacacattt atcctgtttc cctgttccct ttttgatgta ttcgagcata aataaaatac
    28861 cttcgttacc tactatcttc tgtacttggt aaaccttagt caattgcctt ttttaacgct
    28921 tgacgaaaag acatatgatc tttctccgta aagggttttg gaccctttgt ccttatgcca
    28981 gcttttctta actttccact atgataacgt tgttctagaa gcgtgccgat attcgcttcg
    29041 tcaaattctt ttccagagtg atggaacact cgcacaccct tcggcaatac aagtgaagct
    29101 aaaccataat cttgcgtgat caaaaagtca ttcttcttaa ccataccgac gattttataa
    29161 tccgcactat cagctccgcg gtctacgtag acgatttgga catgattagc gtattctttg
    29221 gtcgtataat ggtccacact ggtcacgata accacgtcaa gaccatagga ttctcctaat
    29281 tttatgacat cttcttttac tggagaacca tctccatcaa tgaatagcct cattcttttt
    29341 cgccttcttc cataaatgat aaacagttac taatccgctc agtaaaagac caacattgat
    29401 ccattttgcc aagataagca gttgtacact ttctatcggc actgattcat ggttcagcgt
    29461 gatcaataat agatggattg gctctcccat ttcttttgct aaaacaaaca aaagcgtcat
    29521 caataaaaac tggtggatca cactcccttt attttgagta gtatacttga caacgatccg
    29581 gcgatccatt ggaatagctg tatcagtttg ataatattcc ttatttagcc agctgcctgt
    29641 tactacgtgc cagaaactaa gcagaaagac acctatcgca aaaccatttg gaatcaagag
    29701 gaccaataaa gaagaactca gtccaagcaa ttgcatagcc aatggagacc atgttaaacg
    29761 tttcaaaatc catggtccga ctatttttgc tgcaatcaag cctgctgcat aaggcaaata
    29821 cataaaaatc gaaagttttt ctaatccgta cagtatgcca acataaaaac tgctaaataa
    29881 aaaaaggaaa ttccctagca ttccatttag aaaagtaaac agatttagcc agcttggcaa
    29941 cttccattcc ttcttctggt gtcctaaaac aaacacgaac aagatgaaca gcagcaagaa
    30001 accataaata gcaagatcaa aaagcctttc atcgaataac aatcgggcat ttctcaaaag
    30061 aaacaataag ccaaagaata tcaaaaataa aacaagctct cgcgtagcta ccagatttct
    30121 tttgatgtct ttgaaatcca gttcgtaacg cggataatga ctaaccgtat ggtaggccat
    30181 gacgtaaaaa agtgtataaa tagtaaagct tgctgcaatc tgagtcggtg caggcaactc
    30241 aatcgcttga tacaggagaa atagccagat agctgcaaat aagtactttt tcccatccat
    30301 attgataaaa ccttgcgtct tttcgtgaaa attgaccgtg gtattagcag gtacaagcca
    30361 tgctgcactc agccctagca aaacacttga aaaataaaat aaaggaaaat aaaatacgcc
    30421 taatattcca agcaatgaac cagcaccacc aatcagcaga gcgatcatca gcaacgtgta
    30481 actatctaga ccaaatcgta gactgttgac taaaaatagt ccagtcatcc gaaaggcata
    30541 aaacagcgta aatggcaaaa tcgtcagcca attatgcgag tctgttagtc ccaaaaataa
    30601 aagataagga ataaaaataa ccgtgttact taagataaag ggcgttgttt gtgtaaaatt
    30661 acgcccaaat cgtttgagtt tttcgaacat gacaatggct ccttaaacag tcttataact
    30721 aatctgctct tcgattttag cattaaaggg gaaataaacc aattttacag aatatattct
    30781 tgtaaaaaaa aatacagaaa gaagcataag cttctttctg catttttacg gtacttgatt
    30841 actcaagcag acaagccctt ctccacaaaa gtcttgcccg acacccctgc atccggcagg
    30901 tgataaagat agaaatcaat tatcctgtgc ctccaaaatc aattaggagc tctgtaaacc
    30961 agagtccttc tcgacccgtc gcataaattt actttaacac agttcctatg tttatgcgag
    31021 agcttttttc attttataat cgaccatttg aaatccttga ccgatatctg aaataagttc
    31081 ttctgtaatc tcaaatcctt catgcttata aacctcgatt gcttgatgat tcccctgatt
    31141 cactcgcaaa aagatctctc tttcctttgt ttgcgcagca agttcttcat accagcgaaa
    31201 aactgaagaa gtcaatcctt gaccacgtac tttatttagc aaatataatt tacttaaata
    31261 aatttttcca tttatctctt cataagcagt gtaaccgaca gtcttttctt cgctttttaa
    31321 taaaaagtag gacacacctt ctgtcagttc tttttgaatc tgtttttttg actgataagt
    31381 tttcagcatg taatccactt gtttttgacc aatgatcggc acaaaaactt ccggccatac
    31441 ttcttgtatg actgccatca gctcatccag ttcattttct gttactttta ttttttccat
    31501 acacccacgt ccttttttac attataaatg accttgcgct tgatggaata aaaagagcat
    31561 gatcatgcta tacacaccca ccgaccacgg tttcaagaaa atcacaatct gcataaattc
    31621 cctaaaagat aatttcgtca aaccagctac tagacagatc aagtcgtctg gcgcaaaggg
    31681 aagcagcaag gtaacaacta gtactttttt aatatctttc gtttttcttg tcagcaattc
    31741 atcaaatttc tgatactttt ttggcgataa aataagctgg acaaaggcta cgccatagta
    31801 acggacaagc aaaaatccga ttgcttcccc aatcactagg ccaatacaac tatataaaag
    31861 cccaaatcca ttgccaaaca tcagcatgcc cgctactgag gagatccctc ccggcaaaat
    31921 aggaatgatc acttggatca cttgcaacag aacaaatatc atcactgcat acttgccaaa
    31981 ttgctggata aacgcttgca aagacgtgct cgatcgaaaa atcccatgat gataagcata
    32041 aagaatcaac acaaagaaaa aaaacagacc aataatgggc atgatctgaa gacatttttg
    32101 taaaactgat ccttttttag acatctatat tccccacatc ttttttcatt tattaaaact
    32161 gcctcctt
//
LOCUS       DAZKKW010000026        28360 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.026, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000026 DAZKKW010000000
VERSION     DAZKKW010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28360
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.026"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(255..443)
                     /gene="rpmB"
                     /locus_tag="ACG8PO_001168"
     CDS             complement(255..443)
                     /gene="rpmB"
                     /locus_tag="ACG8PO_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008536053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFQ0206509.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            713..1075
                     /locus_tag="ACG8PO_001169"
     CDS             713..1075
                     /locus_tag="ACG8PO_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFQ0206510.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            1127..2809
                     /locus_tag="ACG8PO_001170"
     CDS             1127..2809
                     /locus_tag="ACG8PO_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HFQ0206511.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKEIPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            2926..4962
                     /gene="recG"
                     /locus_tag="ACG8PO_001171"
     CDS             2926..4962
                     /gene="recG"
                     /locus_tag="ACG8PO_001171"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFQ0206512.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKQFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LITGSTKAKERRELLEKLEQGEINVVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            5011..6012
                     /gene="plsX"
                     /locus_tag="ACG8PO_001172"
     CDS             5011..6012
                     /gene="plsX"
                     /locus_tag="ACG8PO_001172"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFQ0206513.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            6134..6376
                     /gene="acpP"
                     /locus_tag="ACG8PO_001173"
     CDS             6134..6376
                     /gene="acpP"
                     /locus_tag="ACG8PO_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFQ0206514.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            6644..7648
                     /locus_tag="ACG8PO_001174"
     CDS             6644..7648
                     /locus_tag="ACG8PO_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0206515.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            7649..8593
                     /locus_tag="ACG8PO_001175"
     CDS             7649..8593
                     /locus_tag="ACG8PO_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0206516.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            8593..9555
                     /gene="opp4B"
                     /locus_tag="ACG8PO_001176"
     CDS             8593..9555
                     /gene="opp4B"
                     /locus_tag="ACG8PO_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease"
                     /protein_id="HFQ0206517.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            9582..10496
                     /locus_tag="ACG8PO_001177"
     CDS             9582..10496
                     /locus_tag="ACG8PO_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0206518.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            10522..12303
                     /locus_tag="ACG8PO_001178"
     CDS             10522..12303
                     /locus_tag="ACG8PO_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HFQ0206519.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            12651..13337
                     /gene="rnc"
                     /locus_tag="ACG8PO_001179"
     CDS             12651..13337
                     /gene="rnc"
                     /locus_tag="ACG8PO_001179"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFQ0206520.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            13357..16938
                     /gene="smc"
                     /locus_tag="ACG8PO_001180"
     CDS             13357..16938
                     /gene="smc"
                     /locus_tag="ACG8PO_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HFQ0206521.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIEAALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            16947..17756
                     /locus_tag="ACG8PO_001181"
     CDS             16947..17756
                     /locus_tag="ACG8PO_001181"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFQ0206522.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            17767..18765
                     /gene="ftsY"
                     /locus_tag="ACG8PO_001182"
     CDS             17767..18765
                     /gene="ftsY"
                     /locus_tag="ACG8PO_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFQ0206523.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            18952..21219
                     /gene="gshAB"
                     /locus_tag="ACG8PO_001183"
     CDS             18952..21219
                     /gene="gshAB"
                     /locus_tag="ACG8PO_001183"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HFQ0206524.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(21308..21760)
                     /locus_tag="ACG8PO_001184"
     CDS             complement(21308..21760)
                     /locus_tag="ACG8PO_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HFQ0206525.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            21910..22860
                     /locus_tag="ACG8PO_001185"
     CDS             21910..22860
                     /locus_tag="ACG8PO_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFQ0206526.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            22853..23812
                     /locus_tag="ACG8PO_001186"
     CDS             22853..23812
                     /locus_tag="ACG8PO_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFQ0206527.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            23809..24564
                     /locus_tag="ACG8PO_001187"
     CDS             23809..24564
                     /locus_tag="ACG8PO_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0206528.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHIGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            24587..25540
                     /locus_tag="ACG8PO_001188"
     CDS             24587..25540
                     /locus_tag="ACG8PO_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HFQ0206529.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(26184..26480)
                     /locus_tag="ACG8PO_001189"
     CDS             complement(26184..26480)
                     /locus_tag="ACG8PO_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HFQ0206530.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            26835..27191
                     /locus_tag="ACG8PO_001190"
     CDS             26835..27191
                     /locus_tag="ACG8PO_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFQ0206531.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            27201..28100
                     /locus_tag="ACG8PO_001191"
     CDS             27201..28100
                     /locus_tag="ACG8PO_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFQ0206532.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
BASE COUNT         9398 a         4980 c         6107 g         7875 t
ORIGIN      
        1 tttaaatttt ttttcatatt tattttccac caaatttcta tgaagttgta ttcagtttat
       61 catatccttg tttttctctg cttaaaaaga gtctagtttt cacaaaaaaa taaaatttag
      121 aataaataac ttcataattt gttcgtataa caaaaataaa cgcaaaaaaa gggtaggaat
      181 caaaagtccc acccttaaaa atttttatta aataaaaaac cgttcactat ctgaacggtc
      241 caagtatcat tcaattaaac gcgttcaact tttcctgatt tcaaagcacg agttgacacc
      301 caaacttttt taggttttcc gtcgaccata acgcgaactt tttgtaagtt aggtttaaca
      361 gtacgttttg ttgagttcat agcgtgtgaa cggttgtttc cactttttgt cttgcgacct
      421 gtaaagtaac aaacttttgc catggtaatt tcctcctttg ccttgatttg gagcaataga
      481 ataattcagc tcataccaga ctaacatatc atatagaatg ggaatttgca agtacatttt
      541 catagataca attgacaatt gagaagaata attgtaaatt aaacaaggtt atacctgaaa
      601 ttttgcaagg tttttgagta ctttgggtaa ctgcctttta tttcaagttg cgctatgata
      661 aaatatgata gaagaaaaat gggagttttt tacccgttaa ggaggctcat tcatggctgt
      721 aaaaattcaa acgtcagctg gaaccattga aattaccaat gatgtaattg caacagttgt
      781 tggtggcgcc gcgacagatg tgttcggtgt tgtaggaatg gccagcaaaa atcaaattaa
      841 agataatatt actgagattt tgcgtaaaga aaattactca agaggagtag ttgtccgcca
      901 agaagataat ggtattgctg tagatgttta tacgattatc agctatggta cgaaaatctc
      961 tgaagtttcc cgcaacgtcc aagaaaaagt gaaatacaat ctagaggcat tattaggcgt
     1021 aactgccaat tcagttaatg tctttgtaca aggtgttcgt gtactgccag actaggcagg
     1081 agactggctg gtaactcagc agtcgatctg aaggaggatt atctaggtga gtgtaacaga
     1141 aataagtgca agccagttcc aggaaatggt ccaggcaggt gcgaatcgtc tgcaagtaaa
     1201 tgcagaatac gtcaattcat taaatgtatt ccctgtgcca gatggcgata cgggaacaaa
     1261 tatgaatcta tctatgacta gtggagctaa ggcagtagta gattcaacct cagaaaaagt
     1321 aggagaatta gcagcaatgc tttcaaaagg attgctgatg ggtgcgcgcg gaaattccgg
     1381 cgttattttg tctcaattgt ttcgtggatt ttctaagcaa attccagatg ttgtctcatt
     1441 aaatgcttct gatcttgctg cagccttcac tcatggagtt gaaactgcgt ataaagcagt
     1501 aatgaaacca gtagaaggaa cgattttgac agtagctaga gaagcagcga aagctggaga
     1561 aaagaaagca aaaagtactg atgatgtcgt tgaagtaatg acagctgttg taaaaggagc
     1621 aaagcgtgct ttagctaaaa ctccagactt gcttccagtc ctaaaagaag tcggtgtcgt
     1681 agacagtgga gggcaaggac tgctgtttgt ttatgagggc tttttaagtg cattgaatgg
     1741 agaattccaa gcggatgaaa catatgaacc ttctccagca gaaatggatg atatggtcaa
     1801 tgcagaacat catcgcagtg tccaaggtca aatggcgaca gaagatatcc attttggtta
     1861 ctgtacagaa atcatggtaa aaatcggaga aggacctaca gtcgatagta cattcgatta
     1921 cgaagaattc cgtaactatt tagatggtat aggagattct ttacttgtgg tcaatgatga
     1981 cgagatcatc aaagtccacg tgcatacaga aaacccagga gaagtcttga attatggaca
     2041 aaaattcggt tcattagtca aaatcaaagt cgacaacatg cgcttgcagc atgagacgat
     2101 tttggaacac gatgaagaag tgaacgagtt tgcgaaaaaa gaaataccac gtatcccgta
     2161 tggtatcgta gcgatcgctg ctggtaaagg cgtccaagag ctgtttgaaa gtttaggcgc
     2221 taattatgtt atcagtggtg gacaaacaat gaatccaagt acggaagaca ttgttaaagc
     2281 gattcaagaa gtaaacgcag ataaagtgat tgtcttgcca aacaataaga acatcttcat
     2341 ggcagctgat caagcagcag aagtagcaga tatcccagta gcagttgttc cttcaagaac
     2401 gatttcccaa ggattgacag ctatgctttc attcaatgat caagcttctt tagaagaaaa
     2461 caaagaaacg atgacagaaa tgttgtctag cgttgtgagc ggtcaagtca ctcacgcgat
     2521 cagagatacc tcaattgacg gtgtagagat caaagaagga gactttttgg gcatgatcga
     2581 tggaaaaatc ctgatctcta acccagatat cacagcaaca gcgttaagta cactagaaca
     2641 aatgatcaat gaggacacag agatcgtaac gatcttggca ggtgatgaag gaacagttga
     2701 acaagcaaat gaatttgctg agaaactaac agaaaaacat cctgatttgg aaatcgagat
     2761 ccaccaagga gatcagcctg tctatcctta tctattttct gctgaataat ttctgtgtca
     2821 gaatatcttt ctaacacagt attcattcat aaaaggcctt cggttagtga tcaggtatga
     2881 tcgctgacca agtccttttt ttgttaaagg agggaggaaa aatttatgcg ttctttagat
     2941 gatccaatca ctatgctggc aggagtcgga gaaaaaagag cagctagtct tgccagcttg
     3001 ggcatccaaa cgattgaaga cttactgact tattatcctt ttcgctacga agatattcag
     3061 gaaagaaatc taaacgagat ccaggatcaa gaaaaggtta ctttgaaagg aatcgtagtc
     3121 tcgcctcctg tgatgaatcg atttggttat aaaaaaagcc gattgcaatt tcgtatgatg
     3181 caagaacatg atgtgttcaa cgtttctttt tttaatcaac cttatctaaa agaaaaagtc
     3241 attttatcgg aggaaatcgc cgtatacggt aaatgggatg caaagcgtaa agccttgaac
     3301 gggatgaaga ttttaggttc tcaaacaatg gatgactttt cgccgatata tcacgtcaac
     3361 aaatcaattc gtcaaacgac attagttgat ctaatcaaaa aagggtttca agaatacggc
     3421 acattgattg aagaaaatct gccaaacaac cttgtggaga aatatcgact gcttgaccga
     3481 gcttctgctg taagaagtat gcattttcca aaagaccacg aagaaaacca tcaagccaaa
     3541 cgacgtgtcg tttttgaaga gttcttttta tttcaaatgc gtcttcaagg attgaaaaaa
     3601 gcagaaaaag cagaaacgaa tggtttggag atcctttatg atgtccaaaa attgaaacaa
     3661 tttactcaaa agctaccttt tgaactgaca aatgcacaaa aacgtgtaac gaatgaaatc
     3721 tgtcgggatc ttcgcagtcc gcagcatatg cagcgacttt tgcagggaga tgtaggtagc
     3781 ggaaaaacag tggttgccgc gattgcgtta tacgctaccg ttacagcagg tttccaaggt
     3841 gcgttgatgg tgcctacaga aatcttggca caacaacata tggaaagctt gcaacagctg
     3901 tttgatccca atgaagtaac catcgcattg attacggggt caacgaaagc aaaagaacga
     3961 cgtgagctcc ttgaaaaact agagcaaggg gaaattaacg tcgtgatcgg cacacatgca
     4021 ttgatccaag atggagtcga atttcagaat ttaggtctag ttattacgga tgagcagcac
     4081 cgttttggcg tgaaccagcg gaaagtactt cgtgaaaaag gctggcgacc agatgtctta
     4141 tttatgacag cgactccgat tcctagaaca ttagctatta cagcatatgg tgagatggat
     4201 gtatctatca ttgatgaact tccagctgga cgtatcccga tcgaaacacg ctgggtaaga
     4261 acacctcaat tggacagtgt actggattgg acgaaaaaag aactggcaag aggacatcaa
     4321 atgtatgtca tctgtccgct gattgaagaa tcagaagcat tagatgtaaa aaatgcagtg
     4381 gaaatctatg aaaaacttcg tgaactattt gcaccaaaat atgaagttgg cttgttacat
     4441 ggtaaaatga agaatcagga aaaagaagta atcatggaag ccttcaaaga caaccagatg
     4501 cagatattag tttcgactac tgtaattgaa gtaggggtaa acgtgccgaa cgcgaccgtc
     4561 atgctgatca tggatgcgga tcgatttgga ttggcacagc tgcaccaact tcgtggacgt
     4621 gttggacgcg gaagtgatgc ttcctactgt atcttagttg ctaatcctaa aaatgaacta
     4681 ggtgtggaac ggatgaaaat catgacagag accaccaatg gttttgtact aagcgaaaaa
     4741 gatctggaat tacgtggtcc tggtgaagta tttggcttcc gtcaatctgg tctgcctcaa
     4801 tttgcgattg ctgacttggt gacggatgga aatgttttgg aggtagccag agaagaggct
     4861 gcagcccttt ggcaaataaa ggattggcag atgcttcctg agtatcagcc tttagcgcaa
     4921 agcctccaaa tagaaaatca ggataatcgt tatttcgatt agcagactgt gctatacttt
     4981 atactgatga cggaaaaagg agtggaaaca atgaaaattg ccgtagatgc aatgggcgga
     5041 gatcacgcac ctcaagcaat tgtagaaggc gtggctttag cgcagcaaga ttttccagaa
     5101 gtagaattct tactttacgg aaaagaagca gaaatcaaga aatacttaac aaatgaaaaa
     5161 aacatcacga ttatccatac agacgaaaaa atcaatagtg atgatgagcc agtaaaagca
     5221 attcgacgaa aaaagactgc atcaatggtc cttgctgcac aagcagtgaa aaacggtgaa
     5281 gcagatgctg tattttcagc agggaataca ggcgcgctac tagcagcagg gttgttcatc
     5341 gtaggacgta tcaaaaatat cgaacgccct ggcttgatgt ctaccttgcc agtagtaggg
     5401 aaagaaggtg ccggtttcga tatgctggat cttggcgcta acgcagaaaa caaaccagaa
     5461 catttgttgc aatacggtat tttaggttct ttttatgcac gtaaagtccg aggtatcgga
     5521 cgtcctcgtg tcgcactctt aaacaacgga acagaagcaa caaaaggtag tgaagtgacg
     5581 aaaaaagcct atgaactcct tcagaatgaa acaagcttga attttattgg aaacgtagaa
     5641 gctcgtgagc tactcaatgg tgtggctgat gtagtggtca cagatggatt tacaggcaac
     5701 gctgttttga aatctattga aggaacagca atgaatatga tgaatctatt aaaatctgct
     5761 attctaaatg aagggatcaa aggaaaaatg ggcgctatgc ttttaaaaga cggcttacgt
     5821 tctttgaaag cagaaatgga ttattccaaa catggcggtg ccgttttgtt tggcttgaaa
     5881 gcaccagtca ttaaaactca tggagcaaca ggccccgatg ctgttcgcta tacgatccgc
     5941 caaatccaca caatgcttga gacagatgta gttggacaat tagtcgaaca atttgaaaaa
     6001 agcgaagaat agtatttgga tatctgaagc gatccgttcc attgaaaaaa aggtggacat
     6061 tcctgaaaaa tgccagtaaa attagccatg gtgtaggtaa ttgacaagtg tatgattctt
     6121 ggaggtgaaa aagttgactc gcgaagaagt atttaacaaa gtagcgaaaa ttatttcaaa
     6181 tcattttgag atagataccg ataaagtgac ggatgaattg aacatcaaag atgatttaaa
     6241 agctgattca attagtatta tggaattcgt tttagagctt gaagatgaat tcggtacgga
     6301 aatttcagat gaagacgctg aacaaattga aacagtcggt ggcgcagtag attatatcag
     6361 cagccatctt aaataatttc aaataaaagc aaagaaggat aaaaagctgc atgcagcttt
     6421 ttatccttct ttttttaatc tttttgacac gtaaaagtca tttttctgat aattctggcg
     6481 ataaacagaa attatgttgc aaagtaaagc ggttttctat ataattatta ataattcatt
     6541 acacaaagat aagaataacc tttgttataa aacgatttat tctgtgtaat gtcctttttt
     6601 attaagaaaa acttccattt aggaagcaga gaggggtatt cgattgtccg ataaccaatt
     6661 attagaagtt cagaacctac atacaggatt ccgactcaaa gatgactatt acgatgcagt
     6721 agatgacgtt tcatttactt tagacaaaaa cgagatttta gcaatcgttg gggaatctgg
     6781 gtgcgggaaa agtaccttgg ctactacgat tatgggatta cttgatccta ataacacaaa
     6841 aattacaggt gagattttat ataacgatct gaatttaatc gacctgaacg aaacgctcta
     6901 taataaaatc cgcggaaatg atatcggaat gattttccaa gatccgttgt ctgcattaaa
     6961 tcctctcatg agaatcgaag accagatcaa agaaggactt tcctatcaca caaagatgaa
     7021 tgcagaacag cgtcaagctc gtgcactaga attgctagaa caagtaggga ttcctaatcc
     7081 tgcgagagtt ggacgccaat atccccatga actatcaggc ggtatgagac aacgtgtcat
     7141 catcgcaatc gcaatcgcct gcaaacctcc aatcgtcatt gcagatgaac cgacgacagc
     7201 cctagatgta accatccaag cacagattct tgatttactg aaagaccttc aggaagaaac
     7261 aaaaacaggg atcatcttga tcactcatga cttaggagtc gtagcagaaa tggctgatcg
     7321 agtggcagtg atgtatggcg gacagttcgt tgaagtagca ggtgtaaaag aactgtttga
     7381 tcatcctaag catccttata cacgctcttt actgaactct ataccacaag aaggaaatca
     7441 tgatgcagaa ctccatgtga tcgaaggtgt cgttccttct ctgaaaaaca tgcctcgtaa
     7501 aggttgtcgg tttgcaccac gtattccatg gataccagca agcgatcatg aagaagagcc
     7561 tatccttcat gaagtcgcac cgaaccactt ggtacgttgc tcctgctata agcatttcca
     7621 ctttagagac gaaaaaggag aggtgtagat gacagaatta atcacaatca aagacctgaa
     7681 agttcattac cctattcgca gtggattctt caatcgagta acggatcatg tatatgctgt
     7741 agatggtgtg gattttatta tcgaaaaagg aaagacatat ggtttagtgg gagagtctgg
     7801 ctctggaaaa tctactacgg gaaaagccgt tgtgggatta gaaaaggtaa cagccggaca
     7861 aatcatgtat gaaggaaaag atgtaacgaa gcgaagcaac cgcaaaaaaa tgggttataa
     7921 caaggatgtg cagatgatct tccaagattc aatgtccagt ttgaatccta aaaagcgagt
     7981 gttggatatc attgctgaac ccatccgaaa ttttgaacgg ttaagcgatc aagaagaaaa
     8041 gaaaaaggta aaaggactgc tagatatcgt tgggatgccg gaagatgcgc tatataaata
     8101 tcctcatgaa ttttcaggcg gacaacgtca acgtctagga gtagcccgtg ctgttgctac
     8161 caatccaaaa ctgatcgtag ccgatgaacc tgtatctgct ctggatctat ctgttcaagc
     8221 gcaagtattg aatttcatga aacgaatcca gcaagaatac ggcctcagct atttattcat
     8281 ttcacatgat ttaggtgtag taaaacacat gtgcgacaat atcgcaatta tgtacaaagg
     8341 tcgatttgta gaaatcggta caagagaaga catttataat gacccgcgac atatctatac
     8401 gaaacgtctg ctttcagcta ttccgcaaat cgatgtagaa aatagggaaa gccacaaaga
     8461 aaatcgacga agagtagaac aagagtatat ccaaaaccaa aaagaatact atgatgcgac
     8521 tggtcgagtc tacgatttgc gtacgatcac aactacacac aaagtggcgt tgaaagatgg
     8581 aggtgcaagc taatgtggaa aacgattctg cgacgtgttt tattgatgat cccgcaagtt
     8641 attattttaa gtgttctgat cttcttatta gcccaagcga tgccggggga tccatttacc
     8701 ggtttgatca atccaaatca ggatccggca gtgatcgaac agatgcgaca agcagctggc
     8761 ttgaatgacc catggtatgt ccagtacttc cgttggatcg gaaatgcttt gcaaggcgat
     8821 tttggtcaaa gtttcttata caaacgctct gttgctgact tgatcggcga acggatcatc
     8881 aacactttat atctatcgat cttgacagtt gtccttactt atttgattgc tgtgcctctg
     8941 gggatgcttg ctggccggta tcaaaattca atcttggata agattgtcgt aatctacaac
     9001 ttctttagtt ttgctgttcc tttatttatc ttcggcttga tcatgctatt tgtttttggt
     9061 tatcgtttag gctggttccc aacaagtgga tcacaaacac taggtttgca aggtggattt
     9121 atcactcaat ggatagaccg tttgaaatat attctgttac catcaattac ccaagcattt
     9181 ttggcgactg ccgtcacaat ccaatatctg cgaagtgaag taatcgattc gaaatcgctc
     9241 gattttgtca gaacagcccg ttccaaaggg gtaccgacga ataaagtatt cagtcgacat
     9301 attttccgaa atgcagcgtt gccgatggct tctcaaatgg ggtatgaaat cacttcgttg
     9361 attgctggtt ctgtagttat cgaaaagatt tttggttatc cgggagtcgg aaaactattt
     9421 attgattcca tcggccaacg ggactatacc gtgatcacat ccttagtatt gattttagga
     9481 atcgcgacgc tagtggggac attactttca gatatcatca tgagtatcgt tgacccgcgt
     9541 attcggattc aataaaacca atcagaaagg agaatggatc gatgagtgaa aaaaacaata
     9601 cagcagttca agaaagtatt cctcctatgg ggatacgaat gattgctcga gaatttaaaa
     9661 aagataaaat tgctattttt tcattaggtt tgctagtggt tttattatta gtgatcttta
     9721 tcggtgcgat gctgaccgat caagacaaag tgatgtatgt tagtatcttc gacaaatacg
     9781 ctgcaccagg aacagtttcg acacaaggac aaaagttcat tctgggagca gatgaaggcg
     9841 gacgtgatgt attaggacaa ttgatcatag gtgctagaaa ctctgttacg atcggatttg
     9901 cgatcacgat ccttacttcg atcatcgggg ttggattagg gatcatctct ggctactacg
     9961 gagggatcat tgacaacatc ttgatgcgga tcgttgattt tatcatgatt ctaccgatca
    10021 tgctgatcat tatcgtcttc gtatcgatta tttccagata caatgtctgg tcctttatat
    10081 tcatcatgag tgcattttat tgggtagcta aagcccgatt attccgaagc aagacattat
    10141 ctgaagcaag aagagattat gtcagtgcct caaaaacaat gggaaccaat gattttaaaa
    10201 tcatgttccg cgaaatcatg ccgaatctaa gttcattaat cattactaat ttgacgatga
    10261 actttgcggc aaacatcggt atcgagacaa ctttgacttt cttaggtttc ggcttgccgg
    10321 caaatgtacc tagtttaggt acattgatcg gttatgcaag taacggagaa gtcttagtga
    10381 atcggcaatg gatctggtta cccgcatcaa tccttatttt agttttgatg ttgagtatca
    10441 attatgttgg acaagcattc aagcgttctg cagatgcgcg ccaacggtta ggctagtata
    10501 gaagcgaagg gggaaatagg aatgaagaaa acgatttttg gatttgcagc gctgatgtct
    10561 gctgtcgttc ttgcagcatg cgggggcgga ggtacagata cctctggaaa caactctgga
    10621 ggcaacggca ataacaaaga ggaattaact tttccaattg caacaaaaaa taatgatgat
    10681 gcaatcgaag acggcgaatt agaagctgcg gtcgcaacgg acagtcagtt taaaggattg
    10741 ttccagtggg agttttacca agattcatat gatgcagctt ttatggctcc ttctcatgaa
    10801 ccgctatttg caaacgatga cgatttcaga atcacagacg acggagctgc aacgcttgat
    10861 ctagatgaag atacgaaaaa agcgacaatc aaagtaaaag acaacgtcaa atggtcagat
    10921 ggtgaagatg taacggcgga cgatgtgatt ttcccttacg aaatcattgg gaataaagat
    10981 tacactggta tccgttatga cgacactttc cgtaacatcg taggtatgga agaatatcat
    11041 gatggaagtg ccgacacaat ctccggaatc aaaaaaatag acgacaaaac agttgaaatc
    11101 gaatataaag aagtaaaccc aagcatgctt caatcgggtg gtggtatctg gtcatatgca
    11161 gcaccaaaac atacgttaga aggtgtagca atcaaagata tggaatcaag cgatcaagta
    11221 cgtaaaaatc cagtgacttt tggcccttat tacatgagta atatcgttgc aggagaatca
    11281 gtagaatact tgccaaacga gcactactgg aatggtacgc ctaaattgaa aaagatcacg
    11341 atgaaatctg taccgatggc aagtgcaact gaagcattga actctaaatt atatgatatg
    11401 attttccaaa tgccaacaga tacttacgat acctataaag aaattgctgg ctatacaaac
    11461 ttaggccgtg aacaaacttc ttatacttat ctaggattca agttaggtaa atggaatgca
    11521 gatacaaaga gtgtggaata caatccagat tcaaaaatgg ctgataaatc attacgtcaa
    11581 gcaatgggct atgcattaga taataatgca gtaggtgaac gtttctatgc aggtttgaga
    11641 agcaatgcga cttcattgat tcctccagta ttccagtcat accatgatga tgagatgaaa
    11701 ggattcacac aagatctaga caaagcgaaa aaactattag atgatgctgg ctacaaagat
    11761 gtggatgacg atggtttaag agaagacaaa gatggaaata aactaacaat caactttgct
    11821 tcgatgtccg gtggggaaac agcgcaacca ttggcagatt actatatcca acaatggaaa
    11881 gaaattggtt tggatgtcca atacacaaca ggtcgtctga tcgaattcaa ttctttctat
    11941 gataaattag aaaacgatga tcctgaaatt gatgtttacc aagctgcttg gaatacagga
    12001 actgatccaa atccaagtgg actatgggga ccaaatgctg cgttcaacta cactcgttat
    12061 gaatcagaag aaaatacaaa attgatcaac gatatcagtt caagcaaagc atttgatgaa
    12121 gagtaccaaa aagaagcata tcaaaaatgg caacaatatg catttgaaga agcatttgcg
    12181 attccaacgt tgttccgtaa tgaagtattg ccagtcaacg accgtgtcaa agactgggat
    12241 tggtcttatg atgtaccaaa cccatgggca aatgtttctg ttacagcaga ttcacgttca
    12301 taatcactaa aaatgaccga aaaagggcga tgtccttttt cggttttttg atatttttct
    12361 gtttatttct ctaatattca ggtcaaggcg ctgatttcat gacttctcca ataaacggtg
    12421 ttcaaagtcc agtttctcgg tattaagtca aatttatgca aaaatatgga aagccatttt
    12481 cacaaaattt tttcttcata ctcgaaacta aacgaatttt tcacaacttc tcgtcaatca
    12541 ctaggattat taaagcttta cttacctttt gaccgaacca tttcaaattt gacaaaaaac
    12601 cagtataata aggaagtatc gaaaaaatat ccataaaagg aatgtggaaa atggacaacc
    12661 agctgacaaa agaactaaaa gaaaactata acatcgtttt tcacgatcta aatttgcttg
    12721 aacaagcttt tacccattca tcctatgtga atgagcatcg caatctgcaa ttatcggata
    12781 atgagcgact tgaattttta ggagatgccg ttttagaact gatggtttct cagtatttat
    12841 tccgtttata tcctcatttg ccagaaggaa aattgacaaa aacacgagct gcgatcgttc
    12901 gcgaagacag cttgtcgaaa tttgccaaag aatgtcactt tgatcaatat gttttgttgg
    12961 gcaagggaga agagaattca ggaggacgta cgcgaccagc attgctttgc gacctattcg
    13021 aagctttttt aggcgcgtta tatcttgacc agggattcga cacgacccat gcattcatcg
    13081 aaaaagtgat ttttcctaaa gtcaaagcag gtgctttttc acatgagatg gatcataaaa
    13141 caaaactaca agaagtcttg caaaaatcag gtgatgtatc aatcgaatac cgattgatca
    13201 atgaagaagg tccagctcat gaacgagtat tttggatcga agtttatgta gatgatcaat
    13261 tgattggcac tggacaaggg aaatcgaaaa aattagcaga acaagctgcg gcggaaaatg
    13321 cattggcagc actgtaaaag taagtgaggg aaagctttgt atttgaaacg aattgaaata
    13381 gccggtttta aatcatttgc agacaagaca gtcattgatt ttgaaaatag cgtcacagca
    13441 gtggttggac caaatggcag cggaaaaagc aatattacag aggctatccg ctgggtatta
    13501 ggagaacaat ctgctaaaag tctgcgaggc ggaaaaatgc cggatatcat ctttgccggt
    13561 tccgataccc gtaagcagct gaatattgca gaagtcacag tgattttaga caatagtgat
    13621 cattatctgc cattggagta caatgagatc agtgtaaccc gccgttatcg acggactggg
    13681 gaaagcgagt ttttcatcaa taaacaaagc tgcagattaa aagatatcca agaattgttc
    13741 ttagattctg gattaggaaa agaatcgttc tcaatcattt ctcaaggaaa agtagaagcg
    13801 attttcagta gtaaaccgga agaccgtcgt gggatctttg aagaagcagc tggtgtcttg
    13861 aaatacaagc agcggaaaaa gaaagcagaa cagaagcttt ttgaaacaga agacaacctc
    13921 agccgcgtcc aagacatcat ccacgaattg gaagaacagt taactccttt ggccgcccag
    13981 agtgaggcag caaaggagtt tttacggcta aaagaaacac tgactcaaac cgatgtctct
    14041 ttgatggttg ctgaaatcaa gacagcaaaa aaagattggg acaacaaaca agcacagctt
    14101 gcgaaattca atctagaatt aggcaaacta tctgagtcga tccaagaaca agaatccatt
    14161 ttggcgaagc agcgtaaaga aaatgcgcaa gccgatcgtc tgatcgaaaa aaatcagcaa
    14221 gtattattag atctttcgga aaaactgaag cagacagaag gacaaaaaga tgttttgcag
    14281 gagcggacaa agcatacaca aaaaagctcg caagaatatc aaacttcatt agcagaagca
    14341 caaaaaaagg ttaaacattt tgaaaagctt caagaaagct tgatgaaagc agcagcagaa
    14401 aaagaaacag agatccaaaa agctgaagca aatctcataa aaacacagca agaattagaa
    14461 aaataccaaa aatcaacgaa agaattattg gcagaattaa gagatcaata tgttgatttg
    14521 atgcaagaac aagcagctgt aggaaacgaa ctgaagtatc tagaacgtca atatatacaa
    14581 gaaacagcaa aaagcaagca gacgttagcc aagcaatcgg aagtagaagc atcagtagac
    14641 cgattgatct tacaaaaaga agaattgaca caaaagcagg cgcagctaaa atcatcttta
    14701 actgaaacaa aagagaagtt agaaatgatc caacaaaacg ggaaaaagtt ccaagaagca
    14761 ttagcaaaag aacagcctaa aatgtatcaa ttaatgaatc aagtccagca gctacgtgct
    14821 cgtaagaaaa gccttcaaga aatccaagaa aactattttg gtttttacca aggggttcga
    14881 ttggtcttgc agcataaaca gcagctttcg ggtattgtcg gagcagtggc tgaactgatc
    14941 gatgttcctg cagatttcac gttagctatc gaagcagctt taggtggggc agcccagcat
    15001 gtcatcgttg aaaatgaaaa agatgctcgg caagccatca cgtatttgaa acagcaaaga
    15061 ggaggaagag caacattcct tccactgacg acaatcaaac caagacaatt accggctcat
    15121 atattgacac aagcagcagc tgtagaaggg tttataggga ttgccagtga acaagtcagt
    15181 tatcccgatc aaatccaaac tgtggttcaa aacctgctag gaacgatcct tttagccaaa
    15241 gacttaacta gtgccaatgc aatcgcgcaa acgatccgct atcaatatcg agtggtttcc
    15301 ttagaaggtg atgtgatgaa tgccggagga tccatgacag gtggagcgaa taaacgagga
    15361 aaccaaggca gtttgtttgt acaaaatcaa gaattgaaac agttgactag tgaatttgaa
    15421 gaagcagata aacaactcca agcacaagaa aaaaaagttc aggagctgca acaagaaaca
    15481 gctcgtttag ccgaagaaca agaagttttg cgtacacgag gcgaacaatt acgttttgaa
    15541 gaacaagaag cgacaaatca gctacaaaac atcataaatg aacttgaacg ttttgaaaaa
    15601 gaaaagcaaa tctcaacttt tgagacacgt gaactgcagc agtttatcga agattacgaa
    15661 aagcaaacga acgaattgaa agacaaacaa acagatcttg aatcgcaacg acaaaaaatc
    15721 gacgaagaaa tcaaatcgtt aagtcaagaa agcgaccaga tggaagccag aagagcacaa
    15781 gtacaaagtc aaaaagcaca agaacaagca gatcttgctg tcttgaaaga gcagtttaat
    15841 catctgcaga tccagctccg cggtgcccga gtccaaaaag cagaggcaac cgaacgtcaa
    15901 gaagcgatag aaaagcagtt ggcgacattg acagcagact tttctgatca tgaagtcaca
    15961 gaagaaagtt tggaaaaaca aattaacgaa ttatctgctc agcgagaaac attaaaagcc
    16021 gagttggcaa aagccaaaga gcagcgagat agaaggcaaa aggagatcga taagcttgaa
    16081 gcagtcttgg cagagcgtaa tcgtgagcaa aaagcacgac tgtcggaaca atccaaatta
    16141 gaagtccaaa aagaccgagc tgaaatgatt ttagataatc atttgaccta tctgcagtca
    16201 gaatatcagc tgacatttga gaaagcttcc caagactatc aagaaacaac cgatatcgaa
    16261 gacagtagaa caaaggtttc atccttaaaa gaacagattg aaaaactggg accagtaaac
    16321 ttaaattcaa tcgaacaata cgaacaagtc agtgaaagac atacattctt agcaacacag
    16381 cgagatgatc tgttagcagc gaaaaaccaa ttgtttgaaa cgatggatga gatggatgat
    16441 gaagtgcgga caagattcaa agaagtattt gaagctatcc gtcaagaatt caaaattgtc
    16501 tttccaaata tgtttggcgg cggtaaagct gaattagtac tgacggaacc aactgatttg
    16561 ctgaaaacag gtattgagat tgaagtacaa cctcctggca aaaaactgca aagtctaagt
    16621 ttgctttctg gaggagaacg agcacttaca gccatcgcgt tgctcttttc cattatccgt
    16681 gtctgtcctg ttccgttttg tatcttagat gaggtagaag cagcgttaga tgaagccaac
    16741 gtgaaacgtt ttggtcgtta cttgagcgat ttccaagatg acacacaatt tatcgtagtc
    16801 acacaccgca aaggaacgat ggtcgcagca gatgtactgt acggagtgac gatgcaggaa
    16861 tccggtgtat caaaaatcgt ttctgtacgg atggaagata tcaatgaaga ggcgaaaatc
    16921 aacgaaacga aagtgtagga acgaaaatga tcaaattagt agcaatcgat ttggacggta
    16981 cattattaaa tgaacaaaag ctgatttcag atgagaataa acaagcatta gcacaagcaa
    17041 aacagcaagg ggtcaagatc gttctatgta caggacgccc cttagctgcg atggcgcatt
    17101 atctgcaaga attaggactg gtagatgaag gtgactacag catcactttc aacggcggtt
    17161 tggtacaaaa aaatgatact ggtgaaatca tggaaaagaa agtgatggaa gtatcagata
    17221 tccaacgctt atacaaactg gctcagcaat tggatctgcc actagatgtt ttgtctgaca
    17281 atgtggtttt acagctgcct agtgccccaa ggaagcaatc cttgtacaat gtgttgaata
    17341 agctgctgca attccaacca gcggatataa gcgatattac agatgaattt gtgcttaata
    17401 aggcagtaat cgcttatgac caagaagaat tagatcctaa aatcaaagaa ataccagcag
    17461 aattccatga aatgtatgag atcatcaaaa caagaagtat gcttttggaa tttatgccaa
    17521 aaggtgttac aaaagcttat ggtatttctt tattggcaaa agatcttggt ttgaaacaag
    17581 aagaaatcat ggcgatcggt gatgaggaaa atgatttacc gatgatccaa tatgctggac
    17641 taggcgtagc gatggagaat gctgtgcctt tcgtcaaaga agcagcagat ttcgtgacag
    17701 ccagcaatga agaaaatggc gtagccaaag tgatccaaaa atttgtatta gagtaggggg
    17761 agtcaaatgg gattttttga taaaatcaag cgtgctttta ctggcgaaga gaaaacagaa
    17821 gaagttgaag tacaggagaa gtacgacaag ggtttagaaa agacgcgtaa aacatttggt
    17881 gaacggatga atgagttgtt tgccaatttt cgaacagtcg atgaagactt ttttgaagaa
    17941 ttagaagaaa cacttatcgg tgcagatgta ggttttgaaa cagcgatacg aatcaccgaa
    18001 gcattacgtc aagaagtgaa actcagaaat gcgaaaaaat cggcagaagt ccaaaatgcg
    18061 atcatcgaga aacttgtgga tctctatgaa gcggaaggaa tcaatgaagt aaatgaacta
    18121 aatatccaag aagacggact aacagttatt ttgtttgtcg gtgttaatgg tgtagggaaa
    18181 acaacaagta ttggtaaact tgcccatcag ttcaaaaacg aaggtaaaaa agtattgatg
    18241 gcagcagcag ataccttccg cgctggagca atcgaccagt tagttgtctg gggcgaacgc
    18301 gctggtgttg aagttgttcg tggcaatgca ggaggcgatc cagcagctgt cgtattcgat
    18361 gctttggaaa gagcaaagac agagcaggca gatgttttac tagttgatac tgcaggccga
    18421 ctgcaaaata aagtgaactt gatgaacgaa ttagaaaaaa taaaacgagt aatccagcgg
    18481 gaattaccag aagcacccca agaaacactt cttgtattag atgcaaccac tggtcaaaac
    18541 gcgatggttc aagcaaaaca attcaaagaa acaacagatg tgaccggtct tgttttgaca
    18601 aaattagatg gtacagcaaa gggcggtatc gtacttgcta tccgtaatga attgcattta
    18661 ccagtgaaat tggtcggtct tggtgaagga atcgatgact tggagccgtt tgatccaaat
    18721 gattttgtcg ttggattgtt caaaggatta ctaaaagaag aataacccat gtctaaaaag
    18781 caggagctgt gacaaaagtt gcagctcctt gtttgaatga ttgtttttaa catagctgtg
    18841 tttttgatgc cttcattttt atacttattt gtaagcggtt ctcaaacaat ttcgttcttt
    18901 ttcttagact ttttgattat aattagattc atatgaattg gaggtcactt aatgatgaat
    18961 tttaagcaat tattattgca tgtcaatgcg cgccctttta tcgaccaagc tcgatttggt
    19021 atagaacggg aaggacagcg ggttgatctt gcaggaaatc tagcaaaaac cgatcatcca
    19081 gcaatctttg gcgatcgatc ctatcacccc tatatccaaa cagattttag cgaaacacaa
    19141 acagagatga tcacccctgt taccgattct attcccgaat tatttcagta tctcgctgct
    19201 gtttatgatg tgactgctcg ttctataccg aaagaagaga tgatctggcc attaagtatg
    19261 ccacctgcct taccggaaaa agacgaagag atcattattg caaaattaaa aaatttcgaa
    19321 gatgtcttgt atcgacgtta tttagcaaaa gaatacggga agcgaaaaca gatggtaagc
    19381 ggtattcatt tcaattttga atttggcgat gagttgctaa gaacattgtt cagccatcag
    19441 gaagaatttc aagatttttc cgaattcaaa acagaacttt atttgaaaac agcgagaaac
    19501 tttatgcgct atcgctggat gatcacttat ttattcggtg cttcaccaat gagtgagaag
    19561 aactactttt tagatgaatc acatccgcaa gaacctgttc gcagtattcg caacagtgcg
    19621 ctgggctata cgaatcatcc aaatgtgaaa gtttcctatg cttctatgaa acagtattta
    19681 gcagatatcg agcgaatgat cgaagaaggg aaactttcgg aggagaaaga attttataca
    19741 ccgcttcgtt tccgaggcgg aaagaaagtc gcagatttag caacaacagg tgttcgttat
    19801 attgaattgc ggaatatcga tttgaatcct tatgcaagat tgggtatcaa tccagagcaa
    19861 gttcggttct tacaattatt cctgatgtac atgttatgga cagaagaaaa agaagactgt
    19921 gaccaatggg tagcagaagg tacgactcga aataacaaag tagcactaga acaaccatca
    19981 gatcaaacag aatttcatca agaaggtaga gaaattcttg aaggaatgaa gcaaatgctg
    20041 gttgaattgg attggttgga ttctctttat ttagtagagg aagcattgac tcaaatggat
    20101 catcctgaac aaacgttagc agcgaaactc tatcaagaag cacagctatc gagtcagcaa
    20161 gaagttgctg tggcattggg acatcaatat tataaagaaa gccatgaacg tccctatcag
    20221 ttggccggtt ttcgcgaaat ggaactttct actcagatat ttatgttcga tgccatccaa
    20281 aaaggtgtcc aagtcaaagt attagatgag tcggatcaat ttttgcgtct gcaattccaa
    20341 gaccatgtag aatatgtgaa aaatgcaaac atgacaagta aggacagcta tatcgtccca
    20401 cttatcatgg aaaacaaaac agtaacgaaa aaagtcttaa aagaagctgg gttccgggta
    20461 ccaggaggcg cagaattttc atccatggaa gaagcggtaa aagcttatcc gagatttgcc
    20521 gagcaggcat ttgtgatcaa acctaaatca accaattacg ggttaggtat cacgattttc
    20581 aaggaaggag ctagtctgga agattatcaa gcaggtttag ccattgcttt tcgtgaagac
    20641 agttcggttt tagtagaaga atttatgccg ggaacggaat atcgtttctt tgtgatcgac
    20701 ggagaagtcc aagccatcat gttgcgagtc cctgctaatg ttattggtga cagtatccga
    20761 acagtgaaag aacttgttga agaaaaaaat agtgaccctt tgcgtggaac gaatcatcgt
    20821 gcacctttag aattgatcca attaggtgaa ttagaacaat tgatgttgaa agaacaaggg
    20881 ttgacgatcg aatcagttcc ccaagccaat caaattgttt atctaaggga gaactcaaat
    20941 atcagtacag gtggtgattc gatcgatatg actgatgaat tttctgaagc ttacaaaaaa
    21001 atcgccgttt ctgcggtaga agcactagga gctaagatta gtggtatcga tttgattata
    21061 ccagataaag aaatcgatcc tacaactgat aaaaaagcat atgggatcat cgaagcaaat
    21121 ttcaatccag ccatgcatat gcatgtttat ccttttgcag gaaaaggcag aagattgacg
    21181 atgaatgtat tgaaattgtt atatcctgaa gtattttaaa agaaaaagct tttgacagat
    21241 tgtcctattt gatgtcaatc tgatcaaaag ctttttttgc atgtaaaaat ttatgtgttt
    21301 tttcgtttta aaataaacga tcccagaagc tttttttctt ttttggtttt tctttttcag
    21361 ctggcgaaga gttttcttct gggtattttt tttcgatatc aatcacaggt tcatttactg
    21421 cttcttttgc tgttaagagc aatccgaatt cctcaggatt tgcagtgacg taatcgttga
    21481 ctacagtgaa acggctattg tttttcgttg ctagctggat atattggttt tggacagaaa
    21541 ggggtagagc cccattcaag agtatggaag catctggatg ttttttgagt tcttttaaaa
    21601 agttttcttt gttttttggt tggatcattt cagcaacagt catactaaga tagcagcgtt
    21661 ccctgaatgt ccctaaatat ttatgctgtt cttcagggtt cactaaaggt gtaccataaa
    21721 gtcccttatc caaatggctt tttacatcat cttgtgccat aaaaaatacc ctccttcttc
    21781 tctttgtctt tcttacgatt atagcatatt cccaataata cctaaaacat atgaaaaagc
    21841 ggttgctttt gttcaacgat tcacttaaaa taagaatgaa aatcattctc aattagaaag
    21901 gtggattgga tgcgctacgg acgactagta gcattattag ttattttaag tagtatttca
    21961 ttgttcatcg gagtatcgaa tgtacaattg tcagaactgc tccagttaga tgggcaaagc
    22021 ttacaaatct tgctagtgac tcgtttgcca cgaacgattt gtttgatcct tgtgggagcg
    22081 accagtagta tttgtgggtt gatcatgcaa catttgaccc aaaataaatt tgtttcacca
    22141 acgactgcgg gcacgatgga tagtgcgcgt ttaggtattt tagtggctat gattttccta
    22201 cctggtgcat ccttgattgt ccgatccttg acagcatttt gctttgcttt tgcaggaaca
    22261 ttgctttttt tatcccttac gcgattgttc ctccagaaaa atcaattgat gcttccgttg
    22321 attggagtca tgttagggaa tatcattggt tcagttgcta ctttttttgc ttatcaattc
    22381 cagttggtac aaaatatgac ttcatggctt caagggaatt ttgcgactgt gatacgagga
    22441 aattatgagt tattgtatgc tgttgttcca ttgttgctct tggtttggct atttgcttac
    22501 cgatttacga tcattggtct gggcgaatcg tttgcagtaa atctgggaat agattaccag
    22561 aagatgcagt ttttaggcat tggcttagta tcattggcta gtgcagtcat gttgatcatg
    22621 gtaggaagca ttccttttat cggagtgatc attccgaatc ttgtctctag aatatatgga
    22681 gatcaagtgc acaaaacaat tggcgtaact gctttagcag gtagtttgtt cttagtattt
    22741 tgcgatcttg tcgctcgcgt gttgattttt ccttatgaaa taccagtcag tgtgatcgtt
    22801 ggaatcatcg gtggggctat cttcctttat cttctgataa ggggacgacg atatgcttaa
    22861 aaaaaagcca tctattttta ttggtatgct tgttcttgtt ttatttgtta caggaatgta
    22921 cttgacgatc catacatatg gaaactggca ttttgcatgt gcattgagga gtaagaaaat
    22981 ttttgcattt atccttgtct cgctagctgt cagcagtgcc acaatcagct ttcagacaat
    23041 tacgcataac cagtttttga ctccttcagt attgggactt gagtcgttat atgtccttgt
    23101 ccaaacggtc cttttcttct ttatcggagg agtgacgatg cttcagcaga caggtacagt
    23161 ttttttctta ttgaacgttg ggctgatggt cgggctgagt ctattgttat ttttctttct
    23221 tttgaaaaaa atgagagcga atcttttttt attactgatg tcagggatga ttttaggtac
    23281 gctttttggt agtgtcagta cctttttaca agtcatcatg gatccgaatg aatatgattt
    23341 gcttcaaggc agattatttg caagctttgg caatatccag agtcagtatc ttttgcctgc
    23401 ttctttgatc attatcggct gctggtacct gttatttcga tatcgacatg tgctagacgt
    23461 tttccatctc ggaaatgaac aagcagtaaa cctcgggata gacacagatc gttttgaatt
    23521 tttgatttta tgttgtgtca gtcttttgac agggacatca acggctcttg tgggtccagt
    23581 tactttttta gggtttattg tcgcaaatat cagttatcgt gtcttttcta cttatcgtca
    23641 tggtcagcta tttatcggaa gctttctggt agctatgctg tttctgacag gaggacagct
    23701 cgcagtagag cagattttcg gctggaatac aacagtgagt gtcgtcgttg aatttgtcgg
    23761 aggaatttat tttgtcacaa aattgattcg cgaaaggaag agtatcctat gattgattta
    23821 caaaaggtca ataaactgta ccatggaaaa tcagtggtta aagatgtaca gttaacgatt
    23881 cctgaaaaga agttgactgc ttttatcggt ccaaatggtg ctggaaagtc aacggtgttg
    23941 tcgatgatca gtcgtttgat accaaaagat acaggtgaaa tctatttaga ccataacgaa
    24001 gtaaaggctt ggaaatccaa tgaattagcc aaaaaattat cgatcctacg acaatcgaat
    24061 gaaataaatg tccaaatcac cgtgaaagaa ttagtagaat ttggacgatt tccttatacg
    24121 aaaggaaaat tgacgacaaa agacgaggaa atcgttcagg aatcactgga acacattggg
    24181 ttagaagaaa tagcggatca atcgattgac actctttcag gcggtcaact ccaacgtgcg
    24241 tatattgcaa tggtgctagc gcaagacacg gattatattt tactggatga acctttgaac
    24301 aatctagata tgaattttgc ggttcaaatc atgcagattt taaaaaattt agtacaagaa
    24361 atcgggaaaa caattgttat tgttcttcat gatatcaact ttgctgcaag ttatgcagac
    24421 gaaattgttg caatgaaaga tggacagtta tttacgcaag gaacgaccca agaaatgata
    24481 aaaaaagatg tattggatga attatacgaa atggacatac ggatttgtga gattgaaggc
    24541 cgcagattct gtctttattt ctaaaactaa cattaggaga gaagagatga aaaaattagt
    24601 agtaggtatc ttgattctag catcatttgg tttagcagca tgtggaaata gcaacaacac
    24661 cagtcaggca gacacaaaaa aaagcagcac acaaactgaa actacattga cgataacaga
    24721 tagtaatgga gaccaaatcg aggtgccaaa taatccagaa aaagtcgtcg tattcgacaa
    24781 tggttcttta gatacgatgg atgctcttgg tgttggagat aaggtagttg gagcagctac
    24841 aagcagcctt cctgagtatc tttcatccta taaaaaagta gaatcagcag gtggaataaa
    24901 agaaccagac ttagaaaaaa ttaatcagct tcagccagat ctaatcatca tttctggccg
    24961 acaaagagat tttcaaaaag atctaagtca aattgctccg accattttcc tttctttaga
    25021 tgcgaaaaat ccatgggaat ctttccagca aaatgtcact gctttagggg agatctttgg
    25081 taagcaagaa gaagctaaaa ctcaattaga ggaattatct agtgcaatcg atcagacgaa
    25141 aaagaaagca gaagctactg acaaaaaagc actagtcaca cttgtgaatg aagggcaatt
    25201 atcagcctac ggatctggtt ctagattcgg tttcatccat gatttatttg gctttgaaca
    25261 agcagatgat caaatcgaag catccactca cggtcaaagt gtttcttatg aatatgtgct
    25321 ggaaaaaaat ccggatatct tatttgtagt agacagaaca aaagctattg gtggagacga
    25381 cagcaaagat gatatttctg ccaatgaact ggtagcacaa acgaatgctg gtaaaaatca
    25441 gcagatcatc tcccttgaac cagatgtctg gtatttaagc gggggcggtt tagaatcaat
    25501 gaaactgatg atcgaagatg tgaatcaagc atttaaatag cataaataac agaggattaa
    25561 actctcatac tatgtaacaa taatgtttct tacattctta aatagcaaga gcctatcctc
    25621 aaagaagtta tcaaaaacca tccgtaaaat cgtattgatg attttacgga tggtttttat
    25681 ttatttgaga aatctttttt caacgtgaat tcattatcta catcatacgg gttgattcta
    25741 gaatggggag tggcaaaaaa tagtgtgatc cattttttca gcatcaagat cgcctccttt
    25801 gatttgaatc aaaggtacaa agcgaaactt agataaaact taaatttaga tatttttatt
    25861 ttgtaccgat ctcttcttta aacgatgttc catctgctgc ttctcggcaa taagtccact
    25921 agactagaaa atatgagaag ctattttctg tccagcgttc ttattactca aaacaaatcg
    25981 cagttgtcac aatctcttct aaacgatgtt ccatctgctg cttctcggca ataagcccac
    26041 tagactagaa aatatgagaa gctattttct gtccagcgtt cttattactc aaagcaaatc
    26101 gcagttgtca caatctcttc taaacaatgt tcaatcagct gaccttcggt attagctcaa
    26161 agcactgatt tcacaacctc tatttaggca tgtgtctcag caagcggttg atagttgatg
    26221 atctgcattt ttcgataaaa atcggcggga agcttactcc accgttttag taattgatag
    26281 aaataactct cttctgcttt gattttatac tcgcttaata tcaatccaaa actattcgtt
    26341 ttttttgaag ctaatttttt ataaatatcc aatgctttgg ttttatcttc attgacaaag
    26401 agtgttttta cttcaatata agaaccctcg atattttgtg tttcttcgat aaaatagatg
    26461 ttttttatat attccttcat agtgattcct ccaaagaagt cttttatttg ataagtttac
    26521 tctaccgaag agttgttaat aatccgtgaa caaaaagtaa ttctttttcc aatagttgtt
    26581 aaaggttttt tattttttat agtatttttt gttatttaag aaaggaataa ggttaaacaa
    26641 ataacaggtt gctttttggg tctttttccg ttcaagaaac tgaacgaaga aaagggaaag
    26701 ttgtctcttt tgtgataaga gaaatcataa taaacgaatg acttactttc caataaattg
    26761 agaacagtca gaaccagaca aattagttgc atctgatgaa taaagtgatt attctatgac
    26821 atgaggtgat acagatgaaa ttagaagtga caaaagaagc acaagaagta ttgaagaata
    26881 aattagaagc agacgatcaa cttttattag atatcgaaaa cggtgacggt ccatttgcag
    26941 aaagccaagt cagttgccaa ttggatacgg catttcgatt gatcattgtc aaaaaagata
    27001 cacaaacaga tctaagtctt tattctgtag tggctgatac accagttgga ccaatcaaaa
    27061 tcaaagacag tgcaatactt tacatggatg atccatccac acttgcttta gaaccaactt
    27121 ataatagttt acaattaaaa ggaccaagcg gcttgagaaa aggcaacttg caagttgttc
    27181 gaaaagacta gagggataaa atgggaaaat tggcgatcat cagcgaccta catgtggaca
    27241 tcaataaatt cggcgaatca gagctgatgc agctttggca agttttgcaa aagcacaaga
    27301 tcactcggct tcatctagcc ggagatacgg ctaataaagt agaccgttgc ctagctgttg
    27361 tcgaattttt cgtcaataaa ggattatcta cgacatttca tttcggcaat cacgagttgg
    27421 cagatgtgcc caatgaacag atgatggacc attttccaga cccacatttt ttaaatgaac
    27481 ggtatattgc tttaaatgag cagaccgttc ttttagggat gaatggctgg tacgactatc
    27541 agttttccga attgaaagat tacgaggcaa tcgtacgaat gaagcgtttg tattggtatg
    27601 atcggttcat ccagcgtggg aaaacagatc tcgaggtcaa tgcagaaatg cttgcagctg
    27661 caaaaaattt gctagaccag ctggaagcaa aaaaacttga ggtgatttta tccactcatt
    27721 tcgttcctaa acgggcattt atcatgtatc aatctgctcc ttatgagcgt tggaacaaat
    27781 taaatgcatt tttaggatca gaatcattcg gagaattact tgaccgttat tcaaatatca
    27841 aacaagtagt ttttggccat acgcatcgtc gctttgaaga taaatccatc ggtcacacga
    27901 tttacagctg cagacctttt ggttattatt atgaatggca gctgacccga gattttgtct
    27961 tgaaagaaca gttgctagac agctatgatc ctatgaaatt acgtgtcctc ttgcgtgccc
    28021 atcacccagc ttttcaggcc tatcaaacac aacatttgca agaggaattt gaacaagcga
    28081 tgacaatcgt tccgtattga gacagcatta gtaatgtgta aaagaaaata ccttgtaaaa
    28141 cataaggata agtaagagca gtagatccat ttatcgtgga tatgctgctt tttttgcgga
    28201 aaataatcag cgaatgttta tttgatcgga tgatttgtaa ctcgcagagt actagatgca
    28261 agcgtgtatt ctttttttga acgataatca caaaaaaaaa tataatatac attttttatt
    28321 taattataat taatattgat tgaagtctag gcagtcaagt
//
LOCUS       DAZKKW010000027        27862 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.027, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000027 DAZKKW010000000
VERSION     DAZKKW010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27862
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.027"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            152..391
                     /locus_tag="ACG8PO_001192"
     CDS             152..391
                     /locus_tag="ACG8PO_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0206533.1"
                     /translation="MARPVLEAMDMDTLTKGRYVQLLARGYSPKEVFKSLSKGTDMEK
                     ERLRKEFDYWRTHNGIKDLKPARPVIKRKKKITQD"
     gene            458..697
                     /locus_tag="ACG8PO_001193"
     CDS             458..697
                     /locus_tag="ACG8PO_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0206534.1"
                     /translation="MNYEEIENRKKVSKEMEEKLLKTMKQKHLKRLSVMQYINDMQIT
                     GKEKACLLGSMKNFEQLRRTYVKKSSNCQLLLEVS"
     gene            783..1454
                     /locus_tag="ACG8PO_001194"
     CDS             783..1454
                     /locus_tag="ACG8PO_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HFQ0206535.1"
                     /translation="MRKWLFGFLGVALIVFCSVFGYVSYQKHEGKVFKQNIEKKMPVD
                     QINEHAKSYKEDATNVNNDMSLGQMLSIQKEAIEMGVNKQAFAQIQIPALGLALPIFK
                     GANQYTLSLGAATYFYEDAEMGKGNYVLAGHNMEMPGVLFSDIQKLSLGEVMDLVSND
                     GVYRYKVTRKFIVHEYFKLIDGVPEENSFLSLPKKGEKPLLTLFTCVYTSQGKERYVV
                     QGELQ"
     gene            1507..3483
                     /locus_tag="ACG8PO_001195"
     CDS             1507..3483
                     /locus_tag="ACG8PO_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFQ0206536.1"
                     /translation="MAINRYVKKAGMLVGMLGIIASCFQGYQVHAEDVTQKTPPEKVN
                     ITVHKLMYDQGTQLNVDIDGIKNDGYTHDQYPTGVTKYNKADYGDVEFTLGNITDQVL
                     PREDSDLTNAKVDEIVQDVEDKGSNSEYVKNATNKITSAVDENGEITFTDQPAYVNSK
                     GNVYVVYESKSAAGLVTQKAKPMVVIAPMTDKTGSGFLKNIHLYPKNIVSKLSFELTK
                     FGDDGAAQSKQTLLKGAKFELYKGEPGKGTKLGDLVSDDQGKLTATDLTLGKYYFVEV
                     PSEVVVGSDQEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNYKAPVIDK
                     TVTNGTGQEHSFQIGDAVNYQGTIHIPTDIAGGTDGITVNGVKSETSPYSVFKWGDTA
                     GQGLSYVAAKANIKVTNKDGSVVLKENTDYKIQNSENGFVIDFIVNNGQVSDTVASLH
                     GQDLQMTYNMYVNDSAAVANPLTNSVDFVYNNNPFNQEEHHEKTKADVVTYGAKFLKV
                     DSGLFGTGIKATPLEGAEFAAKNAEGKYYGGLVDTDKDGVKEAVWVDDVANAAILKSD
                     KEGRFEITGLTEGEYSLEETKAPTGYQKLTEDIAFKVNKNSFKEENRITVKNNQKAAI
                     PLTGSNGFQTYVLISCLLLGAGALSAVVYFKKKA"
     gene            3496..4251
                     /locus_tag="ACG8PO_001196"
     CDS             3496..4251
                     /locus_tag="ACG8PO_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFQ0206537.1"
                     /translation="MIIRKPPKRRVMHKLMIFFWLLSAVAVYLIPAVETNVAKFQQQQ
                     RILAIENLEKTDSSLDVAKIELGDPVGVLTIPSISLKLPIYDGTSDKILENGVGITEG
                     TGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGQKFFIKVNEEQHAYQIYRIEEVK
                     AEELQKNYLDYLMPEKDQDRITLMTCTPKGVNSHRFLVYGERVSFSKEDLKLQKKAKN
                     RKSISLDWRLALLFAFFAFLLFLFIKKIRRRSE"
     gene            4248..5075
                     /locus_tag="ACG8PO_001197"
     CDS             4248..5075
                     /locus_tag="ACG8PO_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
                     /protein_id="HFQ0206538.1"
                     /translation="MNKRKWLFVCVAALFATAAYSQISFADSSTTPTTSQTTDSSTTA
                     TSDTDSSKNTAEETADSSTSSTESAESTSESSSETSEKLQEVEDTQVIKQNIHIQKII
                     SSNRINNQGRQVTQQEGVNGAKFIVYDITNILEEMTQDDNEKNQSVEIIESKLKDRAK
                     KLSYDQLKKVTEGETQTIDDQEGVIDFSIEVPANKKQAYYIVNESSPENISNSEDIVL
                     LTPVSDEKGEFLTDVWLYPKSKKSEPKEEIKKVVSTGVKKNFFDNCWDFFTHLFFND"
     gene            5085..5333
                     /locus_tag="ACG8PO_001198"
     CDS             5085..5333
                     /locus_tag="ACG8PO_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0206539.1"
                     /translation="MKIIKMLVFNLSFLAATILCCGLVFSQLSIYPRYQRLFVHFLNI
                     QHASMPLEVINKLLINPIFMASLFLLAGLVTWVLGKKE"
     gene            5376..7475
                     /locus_tag="ACG8PO_001199"
     CDS             5376..7475
                     /locus_tag="ACG8PO_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HFQ0206540.1"
                     /translation="MENKIHCTTGRVFTLVLFLFLLLFARGTAVQALEKVDPSQIKEN
                     NNLVLYHDSDSLRNIEVTGINGFKILDEYSTKKQKVLAWPKVKDLKSTSKIVAHYQKV
                     GTYLGKEIDCDVTFSNFVAHAGVNQDGLAKTINGKKYYNYLLFDKNIYRGFFMQSINV
                     MNVKLDFKYAGSKTKVALSEPQPEINSKMRSMRAAGIELNDTFLSIYSLNGQYQQRVS
                     WYNDNNESVGERNIDMREFVGYEKMDRLPYFVTTDTACAEYKAPALSTNNLTSKVVGG
                     RAEKAYSSNDHFTDVVGADTYHRSGVTYTLQGASPTFMIGANTNSMMFSFDTALLWTP
                     QPSKPTKEVFNKANTEEAAHNIDKKVIPQGSDVYYHIHQKFDALTVNTMNKYKSFKIT
                     DTFDSKNFDMVSDGKNYDGALWYVEANGTWKKADTGGKLTVSGNTVTYTASDSFLANA
                     ANYGRDYILQLHMRAKNDAKGIAPNVATVTFNNDPNKGTQKTNKVENYFSLKPNKKVE
                     QDGKDVDGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDIALDHYKQFSIQDPVEA
                     RLTYKEGSARVIDMSTGKDITSEGTLTYDSHSRTLKWEASADFLNNNLLDGREIQLIF
                     TAKTPLNEELDIDNQAKVSVESIEKATNVVTIGVKPNLPQVIVPKTGSNHLVLTVIGS
                     ILFLTLASLGYVVLKLK"
     gene            complement(7513..9570)
                     /locus_tag="ACG8PO_001200"
     CDS             complement(7513..9570)
                     /locus_tag="ACG8PO_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HFQ0206541.1"
                     /translation="MKLFNPKTVLAIGTFAASLSLAGSVDAASTNVMYRLYNPNNNEH
                     FYTSSKKERDHLVKIKWGNYEGPAWEAPTSGGHLVYRLYNKGLRDHHYTASWDEVKWL
                     TKNYGWTYEGPAWRSAEKNNKPIYRLFLPGVKSGSHHYTASWKEVQWLTKDYGWKYEG
                     IGWYGADTSKPKVNKSALEALYTSVKGTKKGDYTDNTWNAFQTALNNAKKVLNDSKAT
                     QKQVDSAKNKLDSAYKGLKKKPVPTVNKAELQALYNRVKSTAKGDFTEESWNNFQTAL
                     SNAKKVLDDSKVSQTQVNNAKNSLDAAFKGLQHKPETPKEYMVTINHIDNQTGQVIAT
                     DQAKVTSGSTYTAKAKAFKYSEGAKDNFSYKVIGNDTQSKTITGNTTITFNYNQVHQI
                     NLLCNNNYKNEGRVDILKEQIINVTHGESVTLTAPSIQGYVLDDRVEPTNSVTLNNVT
                     ESQNYIFNYTRKFNVTVNHVNADTNAVLSSESKTVYEGDSFSTPWKNMTDQNYFLCRN
                     DDPTVTVDKNGQRSINNVDQNKTITFKYKKISLDELRNFMRQKELAWLNDYRQQNGVA
                     PMQFNDIVQQAADIRAKELQVSFSHYRPGGGTFQDLLESLGCYGAKGENISQTGLYID
                     DLLSDDGAIDAMVGWKNSPAHNANLLYNNQSILGLGHNFVVDSAGRIGVDNVFISANP
                     IFK"
     gene            complement(9684..9896)
                     /locus_tag="ACG8PO_001201"
     CDS             complement(9684..9896)
                     /locus_tag="ACG8PO_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0206542.1"
                     /translation="MYADKKKIEWLLFDSGKSVRSISIESGVPLTTVSDLANKRSSID
                     NMRFINAMKLNKLAEEIVDINTSTNK"
     gene            10083..10550
                     /locus_tag="ACG8PO_001202"
     CDS             10083..10550
                     /locus_tag="ACG8PO_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0206543.1"
                     /translation="MKKKQLWFVSMFAIFFIILSGCSSNKKSEAKEDNSLLQPGTVWK
                     EEVGGLIHNLKIIDDTTWEYSESVWHPDPVQITVKRQKDYEGLERYKIVDSAGIREFI
                     NKPDSLFIVVPYEKDGVKKIIFLGSSKDEKRTKEKLIHDGSELNKYKLQKTSE"
     gene            10547..10789
                     /locus_tag="ACG8PO_001203"
     CDS             10547..10789
                     /locus_tag="ACG8PO_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5415 family protein"
                     /protein_id="HFQ0206544.1"
                     /translation="MIASLFIYPEGGITVAKVKTPFEKAASKILERRGVSEIQWKKEV
                     LEKAQFDLLRGKDKDLEEYIYQREREKLVFDEIIKH"
     gene            10807..11109
                     /locus_tag="ACG8PO_001204"
     CDS             10807..11109
                     /locus_tag="ACG8PO_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0206545.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQTAATSLKNNLMIFAAVAA
                     VASIALGGIFFFFGRRGAENGKTHVGYVLLGVGVILAAASIVVSMFSMFGQTPQGL"
     gene            11181..13235
                     /locus_tag="ACG8PO_001205"
     CDS             11181..13235
                     /locus_tag="ACG8PO_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0206546.1"
                     /translation="MFFTISGNAKLDKELIRKATKKVGKRTSFLPKTIKYSVIETEEQ
                     LHVLGQVLEQENQSSVKLTLTLHDKEGKIEKAGTEYVFGPLDYQISEGFYPTLMDLIR
                     LDVFKDTDETMSVEESYRNEKLLQVLEEELESDQRKVNQVSEVEVNEEVPWERPIFPK
                     KESKEKETVYVDEQADIPTQKSIFEEENDVMGQLEVDPFEETMEDEETDEVEVDPVEA
                     NEESTWTEGDSQEYIEPLISEEVEKNETEIQKATVFDYILDQYPPEHEWMKEKNKLFL
                     NELYSKYQLPQTYQAFLESKEELISQGKGTLLEQLESVQSTNWHELAQEDLLATFEER
                     QQETDQEINEYATEQSSNWEKVKEELNKEEQRVIEEEVQKIKERYEKKRDNAYSLCVE
                     KINNFTKTNQEALSKEKENLLKERIKERKEEFYRGLRTDKLKISKELNEQLNKLYQTT
                     TEVLMEKHEQIQQELQAQIPKWKKEYEIHQQELKEQEEKQRKKEKEREQLELKKREQD
                     LAEQKMAIEKQRLEQEKEKEAAARKERQEQMDTLRQAQLNYLTHPQPVAAVQTPVNNQ
                     ANLTPSPQRSFNGWMVGCIASLSLLLGGGTVFAFNHINQSSQADSVATTASIKEEAQE
                     QARKEYEQQLEELLNRTNQSTTESSMENSKESDSSKAKEGSSDSTVQSNTSESSESNE
                     VK"
     gene            13235..15847
                     /locus_tag="ACG8PO_001206"
     CDS             13235..15847
                     /locus_tag="ACG8PO_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirD4-like conjugal transfer protein, CD1115
                     family"
                     /protein_id="HFQ0206547.1"
                     /translation="MRVRETTKRKDKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRIS
                     KKIFYSGLVYLPLEYLVTRVPEVFNRLEIVKGSLQGINLFQLQEMPQVFHFDSAGFWN
                     PFTPTTSLGTRLAISTVCVVPAVLMGLKMDMWFRPLADGQKGDNRLMTLKEIQQIYPQ
                     IPETKTTFPGYGGIPITHYKKYWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAE
                     KQSSMVVNDPKTELYVGSKDELEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDI
                     DTATQLTNTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNN
                     LVELLSEIGGFNYYPDPDNEFKQLNALDEFMNQLPPGNIAKKQYGATKIGSDKAKGNI
                     LSTAITGLNPFTLTKIAKMTSQSTIDYKSVGFPKYLDLKLHESLLNQRVVVEFYHKGK
                     KFHEERVKVGYKGFCEINFDCHLVAGDQVCLKYDHQGKEYVAWYEFSQRVLKDEEGNI
                     VYRKKRGETHLPELEKEAVLTLDEEKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHI
                     IVSIFLSQLYKELSTQCVKTKGDKCHRRIHFILDEFGNMPPMDNMEGIMTVTAGRNML
                     WDLFIQSYQQLYAKYGEQNAAIIKENCQTHIYIMSTDDNTIEEFSKKVGNKTVEQENS
                     TINSLGMNTHINRNVDSDRILTPERISTFLEGETIVLAPLKRQDLKGHKIRPYPIFNH
                     GETNLPYAYQYIEDFNPSWDINDIDIHCTHADLDLKALQIDYDYFLPDEYARNLYHGL
                     HDQLEEKNEENMFYKIVQSLENETAKKLLIQFYEEQAADRLQEYVEKLIKNKEISEMI
                     GKRLLLESEKVVKE"
     gene            15844..18375
                     /locus_tag="ACG8PO_001207"
     CDS             15844..18375
                     /locus_tag="ACG8PO_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pLS20_p028 family conjugation system
                     transmembrane protein"
                     /protein_id="HFQ0206548.1"
                     /translation="MIIGGIFSGIADWWSGLFGEGDANPKTVTKVLQDYGNYLQYQDV
                     FSWIWNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNALFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKVKNILVNLFMAMILIFGGSSLIDEGLNISKSFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLATDPNKVESLPTDKRNALTAKNFKTANINGIIT
                     PEQAKGMAGNDKSGLSDIQKDRLKALQYRLELDDNGKEIPVQISDEGLAKYVYTSGYR
                     RYSSKPGIILAGESSLAVAYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSA
                     IEDISQSFLLLAFTILELRIYTLMLSGIGDLHAAGKINGFLYVVALIVLTIALFKGSQ
                     SVTKIFGVDTSLKNGSNSLLSMFALGNIAKNVGSGAKNLASAGKSGLSHLNDIRKNGF
                     GHRKDVTPDNGEEQSDDKATTVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNERGLKGT
                     AEDLTEKAKDTLEDKLDGTRAKELAEAVKNPSEALEKQKEKASEVKEGLKDAYGEGQV
                     NAAVKANTTTEKENQEGIVIPSVKDHQEALHSNENSIEEKNSIEERNSEEGKNGQLPK
                     TDPEQYRYKPNSDLENEKRISDLSKVNQEDKAENEGQLPKTDPEQYRYRSVNSENEKR
                     ISDLPKRNQEVQTDEDSIVLPNDNGIEVGTEKSTKIGLPKAQNLPMKDSRLNQFKDND
                     QSFVHQSPAGTVQMKPSRLPENGNLTTVSYNLSESGETGNASVQNVVTREAAPKPIQN
                     VHTGYKPANVAFTAPTKINVTRETKNRNNALKEQLLRDIKQDIKP"
     gene            18425..18757
                     /locus_tag="ACG8PO_001208"
     CDS             18425..18757
                     /locus_tag="ACG8PO_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0206549.1"
                     /translation="MDKYINMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKMVNNLV
                     HLPLFFALLNYGLWIAIALVIVWRPYNNPGQRQIFAIIESIFFADKNHYHALDPNRNY
                     HGTYKEDE"
     gene            18758..19351
                     /locus_tag="ACG8PO_001209"
     CDS             18758..19351
                     /locus_tag="ACG8PO_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0206550.1"
                     /translation="MRVKGQNSVERKSILQFGKPEERSILDLFPYKSCTEEGVLITRE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLKKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            19514..21208
                     /locus_tag="ACG8PO_001210"
     CDS             19514..21208
                     /locus_tag="ACG8PO_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HFQ0206551.1"
                     /translation="MLEAIKTAFSPQNDQKVLDPLKQLGNREIEILKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIF
                     ATRTYDEASSLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTETQVTGKDPEAYPTFKEFLDFLGQLELPGASQEKK
                     NRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRGVQQCQMYMA
                     MTLIWNQALIQGRRQRRLIKEKQMEEQDFKRFLAMIDECHNGATCSAVKSCGLQVV"
     gene            21896..23773
                     /gene="ltrA"
                     /locus_tag="ACG8PO_001211"
     CDS             21896..23773
                     /gene="ltrA"
                     /locus_tag="ACG8PO_001211"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFQ0206552.1"
                     /translation="MTKETSPKDRKLRHAEYYGMTEIFDKIYSKSLKGDNFKNLMQII
                     ISDNNIVLAYRNIKRNHGSVTRGEDGVTIKDIENLNRNEFVEIVRKRFAHYNPRKVRR
                     VEIPKYNGKLRPLGIPSIWDRIAQQCILQVLEPICEAKFNNHSYGFRPNRSAEHAIAD
                     CMARMNQSHMEYVVDVDIKGFFDEVNHTKLMRQIWSMGIRDKQLLLIIRKMLRAPVVL
                     PNGKLIYPTKGTPQGGILSPLLANINLNEFDWWIANQWEEKDAKELKHHFRKNGAWDK
                     GNIYHKLRKSTTLKEIYLVRYADDFKIFCRNYNEAKKVFYATKMWLQERLKLPISEEK
                     SKITNLSKEESEFLGFTMKLVTKGNKKVVNTHIGTKAQSMIKVKLKKQIKRIQKSPNS
                     KDTFIYIGVYNSMVIGIHNYFKIATHCSVAMRKIARELRPSFEIRLREQGISKEGQYG
                     KYEGYKPYLRSGQIRYIRGFPILPVGYIQHRHPMCKKRNINKYTEEGREAIHKKQQTV
                     EEWKISWLCSNPVLSKRATIEFADNRISKFIAQKGRCAVTGEELILSEMHCHHIIPYH
                     ESKSDSYENLVIVTEEVHRVIHATQSETIEELLKYLKLNPKQKEKLNELRLKVGNEEI
                     S"
     gene            23904..24281
                     /locus_tag="ACG8PO_001212"
                     /pseudo
     CDS             23904..24281
                     /locus_tag="ACG8PO_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
     gene            24281..25480
                     /locus_tag="ACG8PO_001213"
     CDS             24281..25480
                     /locus_tag="ACG8PO_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFQ0206553.1"
                     /translation="MENQNESLIKQYVKRRAKRRLFLWVFGTSAGLITILITVFVTLF
                     LILAAGSIDNSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYKLL
                     PSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKFPN
                     SKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSAYYKNCMSII
                     NTNKLMEYDEFAIKHWREGGGTGGTITGSWGNPFPGSSLDKNSFSGGQLFGKNPGGEF
                     RPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVYQE
                     FANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWRQAQGHAFIDDGTWIDPLP
                     FLNSSKK"
     gene            25496..26140
                     /locus_tag="ACG8PO_001214"
     CDS             25496..26140
                     /locus_tag="ACG8PO_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0206554.1"
                     /translation="MENKTLKIVLTAAISVSLIGNIALFWQLKEEQETNTYKIEEVAK
                     NASDKLKKNSSEAQAKIDKLNQTINEKNQEIDQLKQQDGTKAKEEVTEAQRKTAEEFG
                     QLAIDKSLSRNELTEKLKDVATQEVIDKLSPADDDHQHDDYGSYSFTLGDVQTYAQTS
                     SVKEEQNFVVFVDYTIGNPQFKDVKPQKIKGGLTVTEKQVDGQWKVTDFSYFTR"
     gene            26151..26957
                     /locus_tag="ACG8PO_001215"
     CDS             26151..26957
                     /locus_tag="ACG8PO_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0206555.1"
                     /translation="MSKKKGARQRFKKRKSYLRWLGIFMILGFLILASPIYTHKKVDI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQSLLKELANLNYRVEVKTAK
                     GDYQAIKAKVIKVSDDYFVVKLAHVPEEYIAMRLTIIPEKIDPKVDMQEPQDLIYYVH
                     EDKVKDQVKDEDYEQHAINYKVKGYKKEQKAAQKQMESFQATIDLNEELVKKLKDQLP
                     YQVADDQENTENKINSYQQEIETSKTQMKDQEEIIQKLQEKIDRMTKEKI"
     gene            26979..27209
                     /locus_tag="ACG8PO_001216"
     CDS             26979..27209
                     /locus_tag="ACG8PO_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0206556.1"
                     /translation="MKRTNLADKDTFAEQKVLIKAYLKVVKAKEICREVNLSNTTISE
                     LKNGRKNLDTLKFRSFEALYVAAKKYFTADNK"
BASE COUNT        10135 a         4337 c         5693 g         7697 t
ORIGIN      
        1 ttgaaatcat tgaatttgta gcggataaag aagaataaaa actggaggaa taaattttac
       61 agataaaggc ttagaaggag tcacgacgat taataaaaag gtagtattac cttaggcatt
      121 gagtaaggaa tttatttagg aggaaaatta gatggcgcga ccagttttag aggcgatgga
      181 tatggatact ttgacaaaag gaaggtatgt acagctgtta gctcgtggtt attcacctaa
      241 agaagtattc aaatccctgt ctaaaggaac agatatggaa aaagaaagat tacgaaaaga
      301 attcgattat tggagaaccc ataatggaat caaggatttg aaaccagcac ggccagtaat
      361 taaacgaaag aaaaagataa cccaagactg aagtgtagag cgtatgtgat cataggcttt
      421 tttatttttg caaaaaacaa ggaaggtagg aaaactcatg aattatgaag aaatcgaaaa
      481 cagaaaaaaa gtgtcgaaag aaatggaaga aaagttgcta aaaacgatga aacaaaaaca
      541 ccttaaaaga ttatctgtca tgcaatatat caatgatatg cagataacag gaaaagagaa
      601 agcttgtttg ttaggttcaa tgaaaaactt tgaacagttg agacgaactt atgtgaaaaa
      661 aagttctaat tgtcaactat tgttagaagt ctcatgacat tcattaatag aattcatttt
      721 taaaactttt tctatggaaa tgagaaaaag attttttgtt ttgtctgaaa gggggtagct
      781 ggatgagaaa atggttattt ggttttttag gagtcgcttt aatcgtcttt tgcagtgtgt
      841 ttggctatgt tagctaccaa aaacatgaag gaaaggtgtt taagcaaaat atagaaaaaa
      901 agatgcccgt agatcaaatc aatgaacatg caaagagtta taaggaggat gcaacgaacg
      961 tcaataatga tatgtctttg gggcaaatgt tgtccataca aaaagaagca atcgaaatgg
     1021 gtgtcaataa acaagccttt gcacaaattc agatacctgc acttggttta gcattgccaa
     1081 tttttaaagg ggcaaatcaa tacactttga gtcttggagc ggctacttat ttttatgaag
     1141 atgccgaaat gggcaaagga aactatgtcc tagcaggtca taacatggaa atgccaggtg
     1201 ttttattttc tgatattcaa aagttgtcat taggggaggt gatggactta gttagtaacg
     1261 atggcgtcta tcgttacaag gtaacgcgta aatttatcgt acatgagtac tttaaattaa
     1321 tcgatggcgt tcctgaagag aactctttct tgtctttacc aaagaaagga gagaaaccct
     1381 tattaacgtt gtttacttgt gtttatacgt cacaaggaaa agaaagatac gtggtacagg
     1441 gagagctaca ataacaaggt tttaatcaac gcgagtcttt gaaatatgaa atgaggagag
     1501 taaaaaatgg caataaatcg gtatgtaaaa aaagctggaa tgcttgttgg aatgttagga
     1561 atcattgcaa gctgctttca ggggtatcaa gtacacgcag aagatgtcac gcaaaaaaca
     1621 ccaccagaga aggtcaatat cacggttcat aaattaatgt acgatcaagg aacgcaattg
     1681 aatgttgata ttgacgggat caaaaacgat ggttatacgc acgatcaata tcctacaggt
     1741 gtaacgaaat acaacaaggc agattatggt gatgttgagt ttacgttagg aaatattacg
     1801 gatcaagtat taccgagaga agattctgat ctaaccaacg cgaaagtgga cgagattgta
     1861 caagatgtag aagacaaggg atctaacagt gagtacgtca agaatgcaac caacaaaatt
     1921 acttcagctg tagatgaaaa tggtgagatc acttttactg atcaaccagc ctatgtcaat
     1981 tccaaaggga atgtatatgt ggtttatgaa agtaaatccg ctgctggatt agtcacacaa
     2041 aaagcaaaac caatggttgt gattgcgcca atgacagata aaacaggatc aggattttta
     2101 aaaaatattc atttgtatcc taagaacatt gtttctaagt tgagttttga attaacgaaa
     2161 tttggtgacg atggcgcagc ccaaagcaaa caaacccttt taaaaggggc aaaatttgag
     2221 ttatacaagg gcgaacctgg aaaaggaaca aaattgggtg atcttgtatc ggatgatcaa
     2281 ggaaagctga ctgcgactga tttgacttta ggaaaatact attttgtgga agttccttcg
     2341 gaagttgtag tgggatcaga tcaagaacca acagcagatc aatatttatt aggcgcagac
     2401 gctcgcaacg atgcgcataa taaattaacc tttgaaatta ccaatgacgg cgtgacaagt
     2461 gacttaaaag cttcctacgt gaactataaa gcaccagtga tcgataaaac agtgacaaat
     2521 ggaacaggac aagaacattc tttccaaatt ggtgacgcag tgaattatca aggaacgatt
     2581 catatcccta cagatattgc aggtggaaca gatgggatta cagtaaatgg tgtgaagagt
     2641 gaaacttctc cttacagcgt atttaagtgg ggagatacag caggacaagg actttcttat
     2701 gtcgctgcta aagcaaatat caaagtaaca aataaagatg gatctgtggt attaaaagaa
     2761 aatacagatt ataaaatcca aaattcagaa aatggctttg tgatcgattt tatcgtgaat
     2821 aatggccaag taagtgatac tgtagcaagt ttacacggac aagatcttca aatgacttat
     2881 aacatgtatg taaatgacag cgcagctgtt gccaatccat taacaaatag cgtggatttc
     2941 gtttataaca acaatccatt taatcaggaa gaacaccacg aaaaaacgaa agctgacgtt
     3001 gtgacttatg gtgcaaaatt cttaaaagtc gatagcggac tatttggaac aggaatcaaa
     3061 gcaacaccgc ttgaaggcgc agaatttgct gctaaaaacg cagaaggaaa atattatggt
     3121 ggattagttg atacggataa agacggtgtc aaagaagcag tatgggtaga cgatgtagca
     3181 aatgcagcga tcctaaaatc agataaagaa ggtcgctttg aaatcactgg tttaactgaa
     3241 ggtgaatatt cactggaaga aacaaaagca ccaactggtt accaaaaatt aacagaagat
     3301 attgcattta aagtaaacaa aaactcattt aaagaggaaa atcgaatcac tgttaaaaat
     3361 aatcaaaaag cagctattcc attaacaggt agtaatggtt tccaaacgta tgtattgatc
     3421 tcttgcttgt tgctaggagc aggggcactt tctgcagttg tttactttaa gaagaaagcc
     3481 tagaaggatc agctaatgat tattcgtaag cccccaaaaa gaagagtcat gcacaaactg
     3541 atgatatttt tttggttgct gtcggcagta gcagtctatt taattccagc tgtggaaaca
     3601 aatgtagcga aatttcaaca acagcaaaga attttagcca ttgaaaatct tgaaaaaaca
     3661 gattcttctt tggatgttgc gaaaatagag ttaggcgatc ctgttggtgt tcttacaatt
     3721 ccaagtattt ctttaaaact accaatttat gacggaactt ccgacaaaat tttagagaat
     3781 ggtgtaggga ttacggaagg aactggcgat attacaggag gaaacggtaa aaatccttta
     3841 atcgcggggc acagtggact ttataaagat aatcttttcg atgatttgcc ttcagtaaaa
     3901 aaagggcaaa aattctttat taaggtaaat gaagaacaac atgcctatca aatttatcga
     3961 attgaagaag ttaaagcaga agaattacaa aaaaattatt tagattatct catgcctgaa
     4021 aaagaccaag ataggatcac gttaatgaca tgcacgccaa aaggtgttaa ctcacaccgc
     4081 tttttagtgt atggtgaacg agtgtcattc agtaaagaag atttaaaact acagaaaaaa
     4141 gcaaaaaata ggaaatcaat ttcattggat tggcgattag cactgctatt cgcttttttt
     4201 gcgttcctat tatttttatt tataaaaaaa ataaggagga gatcggaatg aataagagaa
     4261 aatggctgtt tgtttgtgta gcagctctct ttgcaactgc agcttactca cagatatctt
     4321 ttgctgatag cagtactact ccaactacca gtcagacaac agatagttca acaactgcaa
     4381 caagtgacac ggacagttcg aagaacactg cagaagaaac ggcagattca agtacgagtt
     4441 ctactgaatc agcggaatca acgtcagaaa gttcttcaga gacttcagaa aaactgcaag
     4501 aagtagaaga tacgcaagta atcaagcaaa acattcatat tcaaaaaatt atttcttcga
     4561 atcgaatcaa caatcaagga aggcaagtga ctcagcaaga aggtgtcaat ggtgcaaagt
     4621 ttattgtcta cgatatcacg aatatcttag aagaaatgac acaagatgat aatgagaaaa
     4681 atcaatcggt agaaataatt gagtcaaaat tgaaagatcg agcaaaaaaa ctttcatacg
     4741 atcagttgaa aaaagtgact gaaggagaaa cccaaacaat tgatgatcag gaaggcgtga
     4801 ttgatttttc aattgaagta cctgcaaaca aaaaacaagc atactatatc gtcaatgaat
     4861 caagccctga aaacatttca aatagcgaag atattgtttt attgacacct gtttcagatg
     4921 aaaagggtga atttttgacg gatgtttggt tatatcctaa atcaaagaaa tcagagccga
     4981 aagaagaaat taaaaaagtt gtatcaactg gtgtaaagaa aaactttttt gataattgtt
     5041 gggatttttt cactcattta ttttttaacg attaggatga aatgatgaaa ataataaaaa
     5101 tgttagtttt taatctatct tttttagccg caacaatatt gtgttgcggt ttagtttttt
     5161 ctcaattatc aatttaccca agatatcaac gattgttcgt tcattttcta aatatccagc
     5221 atgcttccat gccactagaa gtgattaaca agctcttaat caatccaatt tttatggcta
     5281 gtttgtttct tcttgcagga ttggtaacgt gggtattagg aaaaaaagaa tgagaagctt
     5341 acaatcgttt aacaaaataa tagatggagg gcattatgga aaataaaatt cattgcacta
     5401 cgggaagagt atttacttta gtgttgtttt tgttccttct tctttttgca aggggaactg
     5461 cagtccaggc cttagaaaaa gttgatccga gtcaaattaa agaaaataat aatctggtac
     5521 tttatcatga tagtgattca ttgagaaata tcgaagtaac tgggatcaat ggatttaaga
     5581 tactagatga gtattcaact aaaaaacaaa aagtacttgc ttggccaaaa gtcaaagatt
     5641 tgaaaagcac gtcaaaaatt gttgcccatt atcaaaaggt gggcacttat ctaggaaaag
     5701 aaattgattg cgacgtaact ttttctaatt ttgtagcgca cgctggggtt aaccaagacg
     5761 gattagctaa aacaattaat gggaaaaagt attataatta tttgctcttt gataaaaaca
     5821 tttatcgagg ctttttcatg cagtcaatca acgttatgaa tgttaaacta gattttaagt
     5881 atgcaggatc aaaaacaaaa gtagctttga gtgaaccaca gccagaaatt aattcaaaaa
     5941 tgagatcgat gagagcagct ggtattgagt tgaatgatac atttctatct atttacagtt
     6001 taaatggaca gtatcagcaa cgtgtgtctt ggtataatga caataatgaa tctgtcggtg
     6061 aacgtaatat tgatatgaga gaatttgttg ggtacgaaaa aatggatcgc ttaccttatt
     6121 ttgtcacaac agatacagca tgtgcagaat acaaagctcc tgcgttatca acaaacaatt
     6181 taacttcaaa agtagtggga ggacgtgcag aaaaggctta tagctcgaat gatcatttca
     6241 ccgatgttgt aggagctgat acttatcaca gaagtggtgt aacgtatacg cttcaaggcg
     6301 cttccccaac attcatgatt ggcgcaaata cgaatagtat gatgtttagc tttgatactg
     6361 cattgctttg gacaccacaa ccatcgaagc ctacaaaaga agtgtttaac aaagctaata
     6421 ctgaagaggc agcacacaat attgacaaaa aagtgattcc acaaggatca gatgtttact
     6481 atcatattca ccaaaagttt gatgcattaa cagtcaacac aatgaacaaa tacaaatcat
     6541 ttaaaatcac tgataccttt gacagcaaaa attttgatat ggtatcggat gggaaaaact
     6601 atgatggcgc attgtggtat gttgaagcga atgggacttg gaaaaaagct gatacaggag
     6661 gaaaattaac tgtatcaggt aatacagtca cttatactgc ttctgacagc tttttagcta
     6721 atgctgcaaa ttacggtagg gactacattt tgcagctcca tatgagagca aaaaatgatg
     6781 caaaaggaat tgcaccaaat gtggctactg taacgtttaa caatgatccg aacaaaggaa
     6841 cacaaaaaac aaataaagta gaaaactatt tctctcttaa accaaataaa aaagtggaac
     6901 aagatgggaa agatgttgat ggtcgaaatg atggtaaaaa aggaacgcct acagctcctt
     6961 taaatgcagg atctgaagta caatatctag tcacacaaaa atggcataca aaaggtgtag
     7021 acattgcgct cgatcattac aagcaattta gtattcaaga tccagtggaa gctcgtttga
     7081 cgtataaaga aggttctgcg cgagtgatag atatgagtac aggaaaagat attacttcag
     7141 aaggtacact aacctatgat agccattcaa gaacgctgaa atgggaagct tccgctgatt
     7201 tcttaaataa caatctttta gatggacgag aaattcaact gatatttaca gctaaaactc
     7261 ctttgaatga agaattggat atcgacaacc aagcaaaagt ttcagtagaa agtattgaga
     7321 aagcaactaa tgtagtaacg attggggtaa aacctaactt acctcaggtc attgtgccaa
     7381 aaactggatc gaatcattta gtgctaactg ttattggaag tatattattt cttactttag
     7441 catcattggg ctatgtagtt ttaaaactca aataaatata aaaacaaggc tgattttcag
     7501 ccttgttttc ttttatttga agattggatt cgcactaata aatacattat ctacgccaat
     7561 tctaccagca gagtcaacta caaaattatg acctaatccg agaatacttt ggttattata
     7621 aagtaaattt gcattatggg cagggctatt tttccatcct accatggcat cgatagcacc
     7681 atcgtctgat aaaaggtcat caatatacag cccagtttga cttatgtttt ctcccttagc
     7741 accatagcag cctaagcttt ccaataaatc ttggaaagta ccaccaccag ggcgatagtg
     7801 actgaagctg acttgtaatt ctttagctcg aatatctgca gcctgttgaa caatatcatt
     7861 gaattgcatt ggggcaacac cattttgttg gcgataatca tttaaccaag caagttcttt
     7921 ttgacgcata aagttacgta attcgtctaa tgaaatcttt ttatatttga atgtgattgt
     7981 tttattttga tcgacgttat taatggatcg ttggccgttt ttatccacgg taacggtagg
     8041 atcgtcattt ctgcacaaga aataattttg atcagtcata tttttccaag gagtagagaa
     8101 gctatcccct tcgtatactg tttttgattc actagagaga acagcgttgg tatcagcatt
     8161 tacatgatta acagtcacat taaatttacg agtgtagttg aaaatataat tttgactttc
     8221 agtcacattg tttaaggtta ctgaatttgt tggttctacc cgatcgtcta aaacatatcc
     8281 ttgaatagaa ggagcagtta aagtgacact ttcaccgtga gtgacgttga ttatctgttc
     8341 tttcaaaata tcaactcgcc cctcattttt gtagttgtta ttgcacaaca aattgatttg
     8401 atgcacttga ttataattga atgtgattgt tgtattacct gtgattgttt tgctttgagt
     8461 atcattacca atcactttgt agctaaaatt atctttagca ccttcgctat atttaaatgc
     8521 ttttgcctta gcagtgtaag tagaaccaga agtcactttt gcttgatctg tagcaatcac
     8581 ttgacctgtt tgattgtcaa tatggttaat ggtaaccata tattcttttg gcgtttcagg
     8641 tttgtgttgc aaccctttaa atgcagcatc aagactattt ttagcattgt ttacttgtgt
     8701 ttgagaaacc ttagaatcgt ctagtacttt tttagcattg gataaagcag tctgaaaatt
     8761 attccaagat tcttctgtaa aatctccttt agcagttgat tttactcggt tgtataaagc
     8821 ttgtaattca gctttattca cagtaggaac tggttttttc tttaatcctt tgtaagcaga
     8881 atctaattta tttttggcac tatccacttg cttttgagtc gcttttgagt catttaacac
     8941 tttcttagca ttgtttaaag cggtttggaa tgcgttccag gtattgtcgg tatagtcacc
     9001 ttttttcgtt cccttaacgc tcgtataaag agcttctaga gccgatttat tcactttggg
     9061 tttagaggtg tcagcgccat accaaccgat accttcgtat ttccagccat agtctttggt
     9121 taaccattgt acttctttcc aacttgcagt gtagtgatgt gagccagatt ttacaccagg
     9181 aaggaaaagg cggtagatcg gtttattatt tttttcggca gaacgccagg caggcccttc
     9241 ataagtccag ccgtaatttt tagtcaacca tttcacttca tcccaactag ctgtgtaatg
     9301 gtgatcacga agacctttat tgtacaaacg atagacaaga tggccaccgc tagtcggtgc
     9361 ttcccaggcg gggccttcat aatttcccca ttttatcttt actagatggt cacgttcttt
     9421 tttgctagag gtgtaaaaat gttcattatt atttggattg taaaggcgat acatgacgtt
     9481 agttgaagca gcgtcaacag atccagcaag actaagtgag gcagcaaatg ttccaatagc
     9541 taaaacagtt tttggattaa ataatttcat tttttcaact cctaaattta attaaaaatg
     9601 tttaaatgat tattatatat cggctaaccg atatagtcaa taaaaaataa gaaaagcctt
     9661 atataatttt ggcttttctt attttatttg ttagttgaag tattaatatc aacgatttct
     9721 tcagccaatt tatttaattt catagcattt atgaatctca tgttgtctat actagagcgt
     9781 ttgttggcaa ggtcacttac tgtagttaag ggtacgccag attctatgga tatagaacga
     9841 actgatttac cactatcaaa cagcaaccat tcgattttct ttttatcagc gtacatttca
     9901 atgctccttc ttgttttttc tttattatag ctcttttttg taatgaatca caatatcaag
     9961 agaaaaattt tctatagaaa ggtggatttt tatgagtatt cgttctccgt gtatccaaat
    10021 gaggtttgtt gctactttcg ttatttgata tactaaaaat aaacaacgaa agaaggaata
    10081 aaatgaagaa aaagcaacta tggtttgtct ctatgtttgc aatatttttt attattttaa
    10141 gtggatgttc aagtaataag aaatcggaag ctaaagaaga taatagttta ttgcaaccag
    10201 gaactgtttg gaaagaagaa gtaggtggtc ttattcataa tttaaaaatt atagatgata
    10261 ctacctggga atattctgaa agtgtatggc atccagatcc tgtacaaata acagttaaga
    10321 ggcaaaaaga ctatgaaggt ttagaaagat acaaaatagt agattctgca ggcattaggg
    10381 aatttataaa caaacctgat tcattattta ttgttgttcc ctatgagaag gatggcgtaa
    10441 aaaaaataat ttttcttgga tctagcaagg atgagaagcg aacgaaagag aaattaattc
    10501 atgatggatc agagttaaat aaatacaaat tacaaaagac tagtgagtga ttgctagtct
    10561 ttttatttat cctgaaggag gaatcacagt ggcaaaagtt aaaacaccat tcgaaaaagc
    10621 cgcgtctaaa atcttagaac gccgtggtgt ttctgaaata cagtggaaaa aagaagttct
    10681 tgaaaaagca caatttgacc tattacgagg aaaagataag gacttagaag aatatatcta
    10741 tcaacgtgaa cgtgaaaaac ttgtattcga tgaaattatt aaacattaaa aggagcgaat
    10801 aatcttatgt ttgaatcaat tacaatgaat tttttaactc aaccgaaagt agtagctgca
    10861 gttgatattt ttagagcatt acaaactgcc gcaactagtt tgaaaaataa tctgatgatt
    10921 tttgctgctg ttgcggcagt ggcttccatt gctcttggag gtattttctt cttctttggg
    10981 cgaagaggag ctgaaaacgg gaagacacac gtaggttatg tattgttggg ggtaggagtt
    11041 attttagctg ccgcttctat tgtagtttca atgttctcaa tgtttggaca aaccccacaa
    11101 ggactataaa aaaaacaagt agaaggagta aaaagtacgt ggctactttt tactcctttt
    11161 tttatgaaag gagaatattc atgtttttta cgatttcagg aaatgcaaaa ttggataaag
    11221 agttaataag aaaagcaacg aagaaagtgg gaaagcgtac ttccttttta ccgaaaacaa
    11281 ttaaatattc tgtcattgaa acggaagaac agctacatgt gttaggacaa gtgttagaac
    11341 aagagaatca atcttctgtt aaattgactc tcacgttaca tgataaagaa ggaaaaatag
    11401 aaaaggctgg cacagaatac gtttttggcc cactggatta tcaaatttca gaaggttttt
    11461 atcctacatt gatggattta atccgccttg atgtgtttaa agatacggat gaaactatga
    11521 gtgtggaaga gtcttatcga aacgaaaaac ttttacaagt tttagaagaa gagttagaga
    11581 gcgaccaaag aaaagtgaat caagtgagtg aagtcgaagt aaatgaagaa gtaccatggg
    11641 aacgaccaat atttccaaag aaggagtcaa aagaaaaaga gacagtctat gttgacgaac
    11701 aagctgatat tccgactcag aaatcgatat ttgaggaaga gaacgatgtg atgggtcaat
    11761 tggaggtaga tccctttgaa gaaacaatgg aggatgaaga gacggacgaa gtagaagttg
    11821 atccagttga agctaatgaa gagtcaacat ggactgaagg ggattctcag gaatatattg
    11881 aaccacttat cagtgaagaa gtagaaaaaa acgaaacaga gatacaaaag gcaactgtat
    11941 ttgattatat tttggatcaa tatccgccag agcatgaatg gatgaaagaa aaaaacaaat
    12001 tatttttaaa tgagctttat tcgaaatatc aattacccca aacctatcaa gcatttttag
    12061 agagtaaaga agaattaatc tcacagggta aaggcacact tttagaacag ttagagtctg
    12121 tccaatcaac aaattggcat gagcttgcac aagaagattt attagccacc tttgaagaac
    12181 gtcaacaaga aacagatcaa gaaattaatg agtatgcgac ggaacagtcg tctaattggg
    12241 agaaagtaaa agaggaacta aataaagaag aacagcgagt gattgaagaa gaagtacaaa
    12301 aaatcaaaga gcggtacgaa aagaaacgcg acaatgctta ttccttgtgt gtagaaaaga
    12361 tcaacaattt tactaagacg aatcaagaag cgttatcgaa agaaaaagaa aacttgttaa
    12421 aagaacgtat taaagaacga aaagaagagt tctaccgcgg tttacgtaca gataagctaa
    12481 agattagcaa agaattaaat gaacaactga ataaactcta tcaaactacg actgaagttc
    12541 tgatggaaaa acacgagcag atccaacaag aattacaagc acaaattcca aaatggaaaa
    12601 aagagtatga aattcaccaa caagaattga aagaacaaga agaaaaacaa cggaaaaaag
    12661 aaaaagagcg cgaacaatta gaactaaaaa aacgtgaaca ggatcttgcg gaacaaaaaa
    12721 tggcgataga aaaacaacgg ctagaacaag aaaaggaaaa agaagcagct gctcgtaagg
    12781 aacgtcaaga acaaatggat acattgcgac aagcacagtt aaattatctg acacatcctc
    12841 aaccagtagc tgctgttcaa acgccagtga acaaccaagc aaatttaact ccttcacccc
    12901 agcgttcctt taacggatgg atggtgggat gtattgcaag tttaagcttg cttctaggtg
    12961 gaggaacagt atttgctttt aatcatatca atcaatcttc acaggctgat tctgtagcca
    13021 caacggcgtc aatcaaggaa gaagcccagg aacaagcaag aaaagagtat gaacaacaat
    13081 tagaagaatt attgaatcgt accaatcagt caacaacaga gtcaagcatg gaaaatagca
    13141 aagagagtga ttctagtaag gctaaagaag gatcaagcga ttcaactgta caatcaaaca
    13201 cttccgaaag ttcagaatca aatgaggtga aataatgcgt gttagagaaa caacgaagcg
    13261 aaaagacaaa cgattcaatt tagaaaagaa taggtatctt tctcatttct atcgctatac
    13321 tccctcagga gaagagctac cagaaaaaca tgacaaacgc atttcgaaaa agatttttta
    13381 tagtggactt gtatacctcc ctttagagta tttagtaaca agagtaccag aagttttcaa
    13441 ccgattggaa attgttaagg gaagcttaca aggaataaat ctgtttcaat tacaggaaat
    13501 gccacaggtc tttcattttg attcagctgg tttttggaat ccatttactc ctacaactag
    13561 tttgggtaca cgtttagcaa tttcgacagt ttgtgtggtt ccagctgtac ttatgggatt
    13621 gaaaatggac atgtggtttc gtcctctagc agatggacaa aaaggggata atcgattaat
    13681 gaccttaaaa gaaatccaac agatttatcc gcaaatacct gaaacaaaaa ctacttttcc
    13741 aggttatgga ggtattccaa tcacacatta caaaaaatat tggtacattc aaacagatac
    13801 tgttaatacg tgtattgttg gtacctctcg atcagggaaa ggtcagattg aagtgttagc
    13861 caccattgac aatttatctc gtgcagaaaa acaaagttca atggtcgtta atgatccaaa
    13921 aacagaatta tatgttggta gtaaagatga actagaagct agagggtatg atgtatatgc
    13981 ctttaatatc accgatccac tgcaatcaat gagtgataat ccattggcac tcatcgtgaa
    14041 atactggaaa cgaggagaca tagatactgc aacacagtta accaatactt tcacaaatac
    14101 gatttattat gatgccaacg cttcagataa caaatacttt aacgaaaacg cgcagaaagc
    14161 tgtcaatgcg cttatttttg cgttgttaga acaagctgat caaacaggag attactccaa
    14221 attgacaccg aataatctcg ttgagcttct ttctgagatt ggcggtttta attattatcc
    14281 agatccagac aatgaattca aacagttgaa tgccttagat gaatttatga atcagttgcc
    14341 acctggaaac attgcaaaaa aacaatatgg ggcaacgaaa attggatctg ataaagcgaa
    14401 gggaaatatt ttatcaacgg caatcacggg attgaatcca tttactctaa cgaaaattgc
    14461 aaagatgact tctcaatcaa cgattgatta taaatcggtt gggtttccaa aatacttaga
    14521 tttgaaatta catgaaagtc ttttaaatca acgagtcgta gtagaatttt atcataaggg
    14581 aaagaaattt catgaagaac gtgtcaaagt aggctataaa ggattttgtg aaatcaattt
    14641 tgattgtcat ttagttgcag gtgatcaagt ttgtttaaaa tacgatcatc aaggaaaaga
    14701 atatgtggct tggtatgaat tttctcaaag agtattgaaa gacgaagagg gtaatatcgt
    14761 ttatcggaaa aaacgaggag aaacccattt gccagaatta gaaaaagaag ccgttctaac
    14821 attagatgag gagaaaagta acttattagt agagtctatt cggatgcact ataccgataa
    14881 accaatggct atttttatgc ttacacctga ttatgatcca tctaaccata tcattgttag
    14941 tatttttctt agtcagttat ataaagaatt atctacgcaa tgtgttaaga ctaaaggaga
    15001 taaatgtcac cgcaggattc attttatttt agatgaattt gggaatatgc caccaatgga
    15061 taatatggaa ggaatcatga cggtcacagc tggccgaaac atgttgtggg atctgtttat
    15121 tcaatcttat cagcaacttt atgcgaagta tggagaacaa aacgcagcga ttatcaaaga
    15181 aaattgtcag acacatattt atattatgtc gacagatgat aataccatcg aagaattttc
    15241 taaaaaagtg gggaataaga cggttgaaca agagaacagt accatcaatt cattaggtat
    15301 gaatactcat atcaatcgca atgttgattc agatcgtatc ttaactcctg aaagaatttc
    15361 tactttttta gaaggtgaaa cgattgtact cgctccttta aaacgacaag atttgaaagg
    15421 tcataaaata cggccgtatc ctatttttaa tcatggggaa acgaatcttc cttatgctta
    15481 tcaatatatt gaagatttca atccatcgtg ggatatcaat gatatagaca tacattgtac
    15541 acatgcggac ttggatctta aggctttaca aattgattat gattatttcc taccagatga
    15601 atatgctaga aatctctatc atggactgca tgatcaatta gaagaaaaaa acgaagaaaa
    15661 tatgttttat aaaatcgttc aatctcttga gaatgagacg gctaaaaaat tgttaattca
    15721 attttacgaa gaacaagcag cagatcgact tcaagaatat gttgaaaagt tgatcaaaaa
    15781 taaagaaatt tctgaaatga ttgggaaacg gttgttattg gaatctgaaa aggtggtgaa
    15841 agaatgatca ttggaggaat tttttctggc atagccgact ggtggtcagg cttattcgga
    15901 gaaggggatg caaaccccaa aaccgtaacg aaagtcttac aagattatgg taattatctg
    15961 cagtatcaag atgtatttag ttggatttgg aacacttttc tgtggggatt gattaaagga
    16021 ttatataatc tcaatacttt actagaaaaa accatttatc aaagctttga tttaaaagac
    16081 ttattgaacg ccgctggggt aaatgcacta tttcaaagtt tgattagtaa ggtattggct
    16141 cttttttgtg tcgtcacatt aatttattta ggcttgaaat tttttatgag taaacaccca
    16201 ccaaaagtca aaaatatctt ggttaattta tttatggcca tgatcttgat ttttggtggg
    16261 agttctttaa tcgatgaagg gttaaatatc agtaaaagct tttacggaga cgtcactgca
    16321 gcaaataaag cagataaaac gggatcacct gcttttcaaa ttattcaaaa taatgtgatc
    16381 gatgtcagta cgttactagc aactgacccg aataaagtag aatccctacc aacagataaa
    16441 agaaatgctt taacagcgaa aaattttaaa acagcgaata tcaatgggat tattactcct
    16501 gaacaagcta aaggtatggc aggtaatgat aaaagtgggt tatcagatat acaaaaggat
    16561 cggttaaagg cattacaata ccgattggaa ctagatgata atgggaaaga aattcctgtt
    16621 caaattagtg acgaaggctt agcaaaatat gtctatactt cagggtatcg tcgatacagt
    16681 agtaaaccag gtattatctt agctggtgaa tcttctttgg cggtggccta tttatttatc
    16741 ctatttacga tcgttacttg cattattgag ctggtattta aaaaattcta tttagtgatt
    16801 gccgcgtcaa cggactttga gacaggccaa cggatgaaaa gtgctatcga agatatttct
    16861 caatccttct tgttattggc atttacaatt ttagaattac gaatctatac cttaatgctt
    16921 tcaggtatcg gtgatttgca tgcggcagga aagatcaatg gattcttgta tgttgtcgct
    16981 ttgattgtac taaccattgc gctattcaaa ggctctcaat cagttacgaa aattttcggc
    17041 gttgatacga gtctgaaaaa cggcagtaat tctcttctta gcatgtttgc cttaggaaat
    17101 attgcgaaaa atgtgggtag cggagctaaa aaccttgcaa gtgctggtaa gagtggttta
    17161 agccatttaa atgatattcg taaaaatgga tttggccatc gaaaagatgt tacccctgat
    17221 aacggggaag aacaatcaga cgataaagct acaactgtac ctaatagtaa gaaaacagtt
    17281 tccgaaatgt tcgggaaagc gaaaaatggt gtttctaaag ccgcagaagg tcttggttac
    17341 gtcaatgaac gtgggttaaa aggaacagct gaagatctta cagaaaaagc caaagacacc
    17401 ttagaagata aattagacgg cacgagagct aaagaattgg ctgaagctgt gaagaatcca
    17461 tccgaagcac tagaaaaaca aaaagaaaaa gcttcagaag tcaaagaagg actaaaagat
    17521 gcgtatggtg aaggacaagt caatgcagca gtgaaagcta atacaacaac cgaaaaagaa
    17581 aatcaagaag gtatagtgat tccttcagtc aaagatcacc aagaagcgtt gcactctaat
    17641 gaaaattcga ttgaagagaa aaattccata gaagaaagaa attcagaaga aggaaaaaat
    17701 ggacagttac caaaaactga tccagaacaa tatcgctata aacctaacag tgaccttgaa
    17761 aatgagaagc gaatttcaga tttatcaaaa gtaaatcaag aagacaaagc agaaaatgag
    17821 ggacagttac caaaaactga tccagaacaa tatcggtata gatctgttaa ctccgaaaat
    17881 gaaaaacgca tttcagattt accaaaacga aatcaagaag tacaaacgga tgaggattcc
    17941 atcgttctgc caaatgataa tggaatcgaa gttggaactg aaaaatcaac gaaaattggt
    18001 ttacctaagg cacaaaattt accaatgaaa gatagtcgtt tgaatcaatt caaggataat
    18061 gatcaaagtt ttgttcatca atcacctgca ggtactgttc aaatgaaacc aagtcgtttg
    18121 cctgaaaatg gaaatctgac aactgtttct tacaatttat ctgaaagtgg tgagacaggg
    18181 aatgcgtccg ttcaaaatgt tgtgacaaga gaagctgcac caaaacctat tcaaaacgtt
    18241 catacaggtt acaagccagc aaatgtggca tttactgcac caacaaaaat aaatgtgaca
    18301 agagaaacta aaaacaggaa caatgcattg aaagaacaac tattgaggga tatcaaacag
    18361 gatatcaaac cataaaaggc ctacaagtta ggccttttat ttatatgaaa aggagaaatc
    18421 aattatggat aaatatataa acatgaaaaa ccctccaaga tttagtggag aatggaagtt
    18481 tttcgctttt ttctttttag atctattaac gcttggtggc tgcttttttt gggcgaaaat
    18541 ggtcaataat cttgtccact taccactctt ttttgcactc ttgaattatg gcttatggat
    18601 tgcgatagcg ttagtgattg tttggcgacc ctataataac ccaggtcaac gacaaatttt
    18661 tgcgattatc gagtctattt tttttgcgga caagaaccat tatcatgcgt tagatcctaa
    18721 ccgaaattat catggtacgt ataaggagga tgaataaatg cgagtcaaag gacaaaattc
    18781 agttgaaagg aaatccattt tgcagtttgg aaaaccagaa gagcgctcta tcttggactt
    18841 atttccgtat aaatcctgta cagaagaggg agtcttaatt actagagaag atcattttca
    18901 acgttattat cgtgtagttt ctacagacgt ggaaggatta aatgaacaag agaaactaga
    18961 acgaatgaat cagttgacca ccgtcatgcg tacgtatgtg ccaacaatta agttagttag
    19021 tttgaccaca gagacggatc taacggaaca aatcgtgcaa aagcggcaat tattagataa
    19081 aaatcgtatg gaacagtcct taggaagaaa tctacaacga ttaaaaaaat atgaaaagaa
    19141 attagtcgaa gaaatccagg aattgaaacg agcagaacaa gaacgtcctg acttaagctt
    19201 ctttttcgtc attgaaggaa agacaatcaa ggatttacaa accagaaacc gtcagttaat
    19261 gcgatctagt ggcattttag ggttaaaacc actctacaaa aaagaattga tcaagattct
    19321 gtatcgaatg aacaatatga atgatgagta ggaatggaga agagagaatg aaatatccgt
    19381 tgatatggtt agaaaaagat gttccgaagg aagagaccac tagaaaattt cctcacactt
    19441 taagcctggg taagttggga aaagggcaat cttttaacca agtaaagaat caaacaagaa
    19501 gaggtgactg tacatgttag aagcaattaa aacagctttt tcaccacaaa atgatcaaaa
    19561 agtacttgat ccattaaagc aactaggaaa tcgggaaata gaaattttaa aatccaaagg
    19621 gtatgattta gattttttaa aacaagtgat gccacaaggt gggttaaatt ttgatgaaga
    19681 atatgtgacg tatggtaatg gttattcacg atgtgtgaca gtttatcact acacaaaaaa
    19741 tcctacgctt ttttggttgg ccaatctcat gaacaatcct catacagtgg ccacactcga
    19801 tgttagcaca gatgaaaagc aaaaagtgat tgatcaaatc aatcgtgcct tagatgaatt
    19861 aaatgatcga tcagataatg aacgaaaagc gactgaccga aataaaagcg cagaagaata
    19921 cctggaacta gcggaatatg ctcgtaaatt gacttctaat ggggaaattt ctaagcaagt
    19981 caaagtgaga gtacatgttt acggaaacac gattcaagaa gtagatgaac gagcgcagga
    20041 actgatcgaa aatctaaaag ggatggatta tcgtgcggtg acttatctgc ataaaaccaa
    20101 acgagattgg caatcgatgt ttaccatgtt cgatgaacaa gctgaatggt tcggaaatat
    20161 caaagggcaa tcactaccat cgttaaacat tggcggaggt ttcccgttcc accaccaaac
    20221 gctaaaagat ccaggaggga cacaatatgg ggaaaccatg acaggcggcc catttgtctg
    20281 gaatgcgacg tataaaaata gtgtacggtt gtctactaat accttgcttc taggaatgat
    20341 gggagctggt aaatcgaccg ttcttaaaat gatcgcggaa gcccatttag ctgcaggaga
    20401 ctttatttgg ggctttgaaa agggaaaaga ctttattcct ttcttaaagg aatataacgg
    20461 tattatggtg cgtttagacg gatctgacgg aatgatcaat cctttagaga tatttgccac
    20521 acggacgtat gatgaagcat ccagtttata tgacgatgga tcagtcaaag atttgaagat
    20581 caatgaagca gcgtcctatc aaacacactt agacaaagtg gtctatcagg tacagttagt
    20641 gagtcctcag ctaaaaggaa cgatgaaagc agaatttaag acctacttaa atcgttttta
    20701 tgaagagtat ggaacagtac cacgcggctt tacttcctca aattcgcgtt caaatacgga
    20761 gacgcaagtc acaggtaaag atccagaagc ttatccgacg ttcaaagaat ttttagactt
    20821 tttaggacaa ctggaattac caggtgcttc ccaagagaaa aagaatcgaa aagaagaaat
    20881 ggaatccatc gtagaatcct tatgtgagac gtatggcatg atttttgatg ggcatagtac
    20941 gattcgacac cttgaccaac aacagttggt cttttttgac attgactcta ttacaggctt
    21001 agatcgaggg gtacaacaat gtcaaatgta catggctatg acgttgattt ggaatcaggc
    21061 attgattcaa ggaagaagac aacgacgttt aattaaagaa aaacaaatgg aagaacaaga
    21121 cttcaaacgc ttcttggcga tgattgatga gtgccataat ggtgcgacct gttctgcagt
    21181 gaaaagctgt gggttgcaag tagtctaaaa actattgtaa taactcttat aacgagaagt
    21241 caaatgagtg ggtaacgcca caaagggctt ccactgatac tccgacatgc aacaagtagt
    21301 ttatgcagac caacgttgtg tgaagctcgg tgaagtcggc tgaaggatac cgtaaggtaa
    21361 aagaaatagt gaataccacg aaagttcttt agaggtaaag ggtgtatccg ataagtcggg
    21421 tgtccaaaaa aacttaatat ggtgagtatg tacaaacgta ctgacgaact agcgaatgta
    21481 ctgtccgaaa ggcatagctc ctagaaatgg gagagacagt gagcaaaaaa tcactcattg
    21541 tgctaacttg tggttgagta aaagtcgttg ttatgaaaga ccatgctgcg ttacaggcgt
    21601 aggagaagtt agggggcaaa agctagcatc taagttaagg catgtggata gatataaggg
    21661 aacaggggaa gccatcaaca tggagagtgc atactcgatg aagtggtagt aagaaatatg
    21721 tgaatgacaa ataacttact ttagtgatgg tgaaagtaga gtcacgactg atgaaggttc
    21781 tgtaatggaa ctggaggaac aggctcgatt cgtctgtaaa agtcatgaaa cccgttcaac
    21841 catgggattc tcgtatgact aaaaaggtca cgagccaatt ggagcgtgat aaaccatgac
    21901 aaaagaaaca agtccaaaag ataggaagtt gcggcatgca gaatattatg gcatgacgga
    21961 aatcttcgat aagatatact ccaaaagttt gaagggagat aatttcaaga atctaatgca
    22021 gattatcatt tctgataaca acattgtcct agcatatcgt aatatcaaga gaaatcacgg
    22081 tagtgtgaca agaggagaag atggagtaac gattaaagat attgaaaatc tcaaccgtaa
    22141 tgaattcgta gaaattgtta ggaagcgatt cgcacattat aaccctcgga aagtaaggcg
    22201 tgtagaaatt cctaaatata atggtaaatt aagaccgttg ggtattccat caatatggga
    22261 tagaatcgcg cagcaatgca tcttacaagt attagaacca atatgtgaag caaaattcaa
    22321 taatcacagc tatggtttcc gcccaaatag atcagcagaa cacgcaatag cagattgtat
    22381 ggcccgaatg aatcaaagtc acatggaata tgtggtagat gtagatatca aaggtttctt
    22441 cgacgaagtc aatcatacta aactcatgag acaaatctgg tctatgggta tacgagacaa
    22501 acagcttctc ttaatcatta ggaaaatgtt aagagcaccc gtagtacttc caaatgggaa
    22561 gttgatatac ccaacaaaag gaacaccaca aggcggaata cttagcccat tacttgcgaa
    22621 catcaatctc aacgaatttg actggtggat agcaaatcag tgggaggaaa aagatgccaa
    22681 agagctaaaa catcatttta ggaaaaatgg agcttgggat aaaggtaata tctatcacaa
    22741 gctcagaaag agtacaactc taaaagaaat atacctagtg agatacgcag atgattttaa
    22801 aattttctgt agaaattata atgaagcgaa aaaagttttc tatgcaacta aaatgtggtt
    22861 acaggagcgt ctaaagttac caatatctga agaaaagtca aaaatcacaa atctttcaaa
    22921 agaagaaagt gaatttttag gctttacgat gaaattagtg accaaaggta acaaaaaggt
    22981 tgttaatacg catataggta caaaagcaca aagcatgatt aaagtgaaac taaaaaaaca
    23041 aatcaaacga attcaaaaaa gtcctaatag caaagatacc tttatctata ttggagtcta
    23101 taacagtatg gttattggga ttcataatta cttcaaaatt gcgactcatt gtagtgtagc
    23161 aatgcgaaaa attgcaagag aactcagacc aagttttgaa atacgactac gagaacaagg
    23221 aatctcaaaa gaaggacaat acggaaagta cgaaggatac aagccttacc tacggtcagg
    23281 acaaatacga tatataagag ggtttccaat attaccagta gggtatattc aacacagaca
    23341 tccgatgtgt aaaaaaagaa atattaataa atacaccgaa gaaggtaggg aagccattca
    23401 taagaagcaa caaaccgtag aagaatggaa gatatcgtgg ttgtgtagca atccagtgct
    23461 tagtaagaga gcaacgattg aatttgcgga caatcgaatt tcaaaattca tcgctcaaaa
    23521 gggaagatgt gcagtaacag gtgaggagct tatcttatct gaaatgcatt gtcatcatat
    23581 tatcccttat catgaaagca aaagtgatag ctatgaaaac ctagtgattg ttactgagga
    23641 ggttcatcgg gtaatacacg ctacacaaag tgaaacgatt gaggaacttc taaaatatct
    23701 aaaattaaat ccaaaacaaa aagaaaaact caatgagttg agattaaaag ttggtaacga
    23761 agaaatatct taataaatat ttgtaggacg ggaaaacaga atgtctttta tagacgaggg
    23821 aacgccgtat gcggtgaaag tcgcacgtac ggtgtggact gggggaaaag acggagataa
    23881 tatcaaactc ttacctatca gtatcgatta atccagattt tattgaaacg gtaaattatg
    23941 tcacaaactt tcaacgagaa atgcgtaaag tagaagcaat gactgtttta gctacacaat
    24001 ccccacaaga aatggtgcca gaaaatatgt cttctgataa cttctcgaaa ctgaagaaag
    24061 tctttgaatt ttcttctacg aagatcttta tgcgtatgga tgaatctatt ttacgtcata
    24121 tagaaggatt agtgggtaaa tcaatgacga actcagaatt agaatccatt cctcaacaaa
    24181 atcaaggaga cgctgtgatc aattttggct caaaagaaac cattcgtgtt cattttagtc
    24241 cgaacaaacg tcaattaaaa ttgtttgatg gaggaaaata atggaaaacc aaaatgaaag
    24301 tttaatcaaa cagtatgtta agcgtcgtgc aaagcggcgc ttatttttgt gggtgtttgg
    24361 tacttctgca ggtttgatta ctatattgat cactgtgttt gtgacccttt ttctaatact
    24421 tgcggcaggt tctattgata attctgattc aagtagtggt ggcgaagctt ttacaggaga
    24481 atattcggaa ggcttaccca tttataaaga aatcaaagga agaggccctt tttctgatga
    24541 gattgcgcaa tatgctgtag gtgcagcagt gaagtataaa ttattaccaa gtgtgatcct
    24601 ttcgcaatac ggttatgaat cagcgtttgg tacgtcggcg tccgcaagaa atgatctcaa
    24661 ttattttggg attacgtggt ttgatgggtg tctgtttcct aaaggaactg cgcgtggaat
    24721 tggtggcatt gaaggtggct ggtacatgaa gtttcccaac tcaaaagctg ccttctccta
    24781 ttacggcttt atggtagcca cacaatcaaa ttttaatgct tgtgtcggaa ataaaagccc
    24841 tggcgcaagc ttattgatct taggacgagg agggtatgcc gccgcaggaa ttactgaaga
    24901 cagtgcctat tataaaaatt gtatgtctat tattaataca aataaattga tggagtacga
    24961 tgagtttgcg attaaacact ggagagaagg cggcggaact ggtggtacga ttacaggaag
    25021 ctggggcaat ccattcccag gaagtagctt agataaaaat tcgttcagtg gaggacaact
    25081 ttttgggaaa aatccaggag gagaattccg acctaacggg ttccatgatg gactagactt
    25141 tggttcagtc gatcatccag gaagtgagat tcatgcggtt catggcggaa aagtcgtgta
    25201 cgttggcaat cctggtattt caggtttggg tgcgtgtgtg atcgtgatta attacgatgg
    25261 cttgaatatg gtgtatcaag aatttgcaaa tagtacagga aattctcgag tgaaagtagg
    25321 cgatcaagtg aaagttggtc aagtaattgg gatacgagat acagcacact tgcatttagg
    25381 atttactcga atggattggc gtcaagcaca aggacatgca ttcattgatg atggtacatg
    25441 gatcgatccg ttaccatttt tgaatagcag caaaaaatag atcaggagaa aaaatatgga
    25501 aaataaaacc ttaaaaatcg tcttaacagc tgcaattagc gttagtttaa tcggcaatat
    25561 tgcgttgttt tggcagctta aagaagaaca agagacaaat acctataaaa ttgaagaagt
    25621 ggctaaaaac gcgtctgata agctcaaaaa aaattcttct gaggcacagg cgaagatcga
    25681 caagttaaat caaaccatca atgagaaaaa ccaagaaatc gatcagctga agcaacaaga
    25741 tggcacaaaa gcaaaagagg aagtaactga agcacaaaga aagacggcag aagagttcgg
    25801 tcaattggcc attgataaat cattaagcag aaatgaatta acggaaaaac taaaagatgt
    25861 cgcaacccaa gaagtgatcg acaaattaag tcctgcagac gatgatcacc aacatgacga
    25921 ctacggaagt tactctttta cattaggtga cgtgcaaact tacgctcaaa caagcagtgt
    25981 aaaagaggaa caaaattttg tcgtatttgt tgattataca atcggcaatc ctcaatttaa
    26041 agatgtcaaa ccacaaaaaa taaaaggtgg attaactgtc actgaaaaac aagtagatgg
    26101 tcaatggaaa gtcacagact ttagttattt cactcgttag gaggaagaaa atgagtaaga
    26161 aaaaaggagc aagacaacgt tttaaaaaac gaaaaagtta tcttagatgg ctggggatct
    26221 ttatgattct cggctttttg attcttgcta gtccgattta cacacataaa aaggtagaca
    26281 taccagatga agtaagcacg aacgcatttt taaacacgaa ggaactgacg atggtttcaa
    26341 aagaaaaagg agcgaaccag caattagttt ttgaatttgc gatcgacgat gaagatcaga
    26401 gcttattaaa agaactagct aatcttaatt atcgtgtaga agtaaagaca gctaaagggg
    26461 attatcaagc aataaaagcc aaagtgatca aggtgtctga tgattatttt gtagtgaagt
    26521 tagcacatgt accagaagaa tatatcgcca tgcgtttaac cattattcct gaaaaaattg
    26581 atccaaaagt ggatatgcaa gaaccacaag atctgattta ttacgttcac gaagataagg
    26641 ttaaagatca agtgaaagat gaagattatg aacaacatgc gatcaattac aaagtgaaag
    26701 gatacaaaaa agaacaaaaa gcagcacaaa aacaaatgga atccttccaa gcaaccattg
    26761 atttgaatga agaattagtg aagaagctaa aagatcaatt gccttatcaa gtcgcagatg
    26821 atcaggaaaa tactgaaaac aagattaata gctaccagca agaaattgaa acatcgaaaa
    26881 cgcaaatgaa agatcaagaa gagatcattc aaaaacttca agaaaaaatt gatcgtatga
    26941 caaaagaaaa gatttagaag gacaggtgga gtgaaaaaat gaagcgaaca aacttggctg
    27001 acaaagatac tttcgctgag cagaaagtat tgattaaagc ctatctcaaa gttgtaaaag
    27061 ctaaagaaat atgtcgagaa gtgaatttat ctaatacaac aatatctgag ttgaagaatg
    27121 gtcgaaagaa tttagatact ttaaaattca gatcatttga agctctatat gtagctgcta
    27181 aaaaatattt tacagctgat aacaaataaa aaacaggagt attttatgtg aaaaaagtat
    27241 ataaaaaatg cttatgaatc agctatatca ccttaaccat ttgttaatga gatgggagaa
    27301 aacattaacc acgattctaa cgcaaacatc aagcacataa aacatttaac actcctcctt
    27361 tctggctgaa gatttcaacc aaacaggaag acagcgtatc aattatagca tacacttttt
    27421 cttaaattca tttttcatat gacaaaatat ttccttctaa tcgacaaagt tagaaggatt
    27481 ttttattgtc taaatacaca aataacctta atttataaag agcataagga aacgtgggaa
    27541 cactttttaa aaaaagtgtt ccaataggct gtgcctatta cgtttctcaa aaataattgt
    27601 gggaacaaaa tatttaaaaa taggatagtg aaaggatagc aaaggatagt aaaaggatag
    27661 aagcaggata gcgaaaagga taggctcctt ttcgctatcc tttttaaatt ggaggaatta
    27721 atatggaagt aagaataaga aatgtagatg agaaaatcgt aagagaaatt gatcgaagat
    27781 gtgacgaact aagtaaccga acgggtaata aatggagtcg taacgattat ttaaagctac
    27841 tcgttgaaaa tgattttgat cg
//
LOCUS       DAZKKW010000028        27860 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.028, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000028 DAZKKW010000000
VERSION     DAZKKW010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27860
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.028"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(405..1349)
                     /locus_tag="ACG8PO_001217"
     CDS             complement(405..1349)
                     /locus_tag="ACG8PO_001217"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFQ0206557.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(1645..2589)
                     /locus_tag="ACG8PO_001218"
     CDS             complement(1645..2589)
                     /locus_tag="ACG8PO_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFQ0206558.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            2810..4024
                     /locus_tag="ACG8PO_001219"
     CDS             2810..4024
                     /locus_tag="ACG8PO_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0206559.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            4046..6061
                     /locus_tag="ACG8PO_001220"
     CDS             4046..6061
                     /locus_tag="ACG8PO_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HFQ0206560.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(6144..7517)
                     /locus_tag="ACG8PO_001221"
     CDS             complement(6144..7517)
                     /locus_tag="ACG8PO_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFQ0206561.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(7543..7827)
                     /locus_tag="ACG8PO_001222"
     CDS             complement(7543..7827)
                     /locus_tag="ACG8PO_001222"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0206562.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(7829..9844)
                     /locus_tag="ACG8PO_001223"
     CDS             complement(7829..9844)
                     /locus_tag="ACG8PO_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFQ0206563.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(10023..10328)
                     /locus_tag="ACG8PO_001224"
     CDS             complement(10023..10328)
                     /locus_tag="ACG8PO_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HFQ0206564.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(10417..11448)
                     /locus_tag="ACG8PO_001225"
     CDS             complement(10417..11448)
                     /locus_tag="ACG8PO_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFQ0206565.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            11655..12530
                     /locus_tag="ACG8PO_001226"
     CDS             11655..12530
                     /locus_tag="ACG8PO_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFQ0206566.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(12682..13524)
                     /locus_tag="ACG8PO_001227"
     CDS             complement(12682..13524)
                     /locus_tag="ACG8PO_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFQ0206567.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(13616..15493)
                     /locus_tag="ACG8PO_001228"
     CDS             complement(13616..15493)
                     /locus_tag="ACG8PO_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFQ0206568.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(15490..16911)
                     /locus_tag="ACG8PO_001229"
     CDS             complement(15490..16911)
                     /locus_tag="ACG8PO_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFQ0206569.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(16914..20303)
                     /locus_tag="ACG8PO_001230"
     CDS             complement(16914..20303)
                     /locus_tag="ACG8PO_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFQ0206570.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            20560..21951
                     /locus_tag="ACG8PO_001231"
     CDS             20560..21951
                     /locus_tag="ACG8PO_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0206571.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(22024..22671)
                     /locus_tag="ACG8PO_001232"
     CDS             complement(22024..22671)
                     /locus_tag="ACG8PO_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0206572.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(22679..23365)
                     /locus_tag="ACG8PO_001233"
     CDS             complement(22679..23365)
                     /locus_tag="ACG8PO_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0206573.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(23371..24444)
                     /locus_tag="ACG8PO_001234"
     CDS             complement(23371..24444)
                     /locus_tag="ACG8PO_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0206574.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            24666..25340
                     /locus_tag="ACG8PO_001235"
     CDS             24666..25340
                     /locus_tag="ACG8PO_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFQ0206575.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            25345..26685
                     /locus_tag="ACG8PO_001236"
     CDS             25345..26685
                     /locus_tag="ACG8PO_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFQ0206576.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(26834..27820)
                     /locus_tag="ACG8PO_001237"
     CDS             complement(26834..27820)
                     /locus_tag="ACG8PO_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HFQ0206577.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
BASE COUNT         8123 a         5263 c         5051 g         9423 t
ORIGIN      
        1 cgaacggttt tttatcaaaa atcgtcattt ttccatcaaa atctgcttat gcaaaattat
       61 aaaaaaattt tgcagttttt cttacttttt ttgtcgttta attgaatgat aaaaaccagt
      121 ttctttattc agttaatcaa atatgaacga attagatatg aatcattgta tttgccgttg
      181 cggaaggatt ttttaattaa ccacagataa acattgataa cgattcgctt attgtaatca
      241 acagattaaa tttcatatgt tcatatgtac ggatacattt tcggaccgat taattttaac
      301 cattctgttt atttgcttac ctattagata tcaataaaaa actgcttaac agctatttca
      361 gctgttaagc agttttagga tttttagttc atattcattg tttcttataa tacagtgttc
      421 aatacttctc ttaaagcact tgttgatttg ctgaacattg ctttttcttc ttcgttgatt
      481 gataattcaa caacttcacg aacaccattt tcgtcaacga ttgatggcac acctgtaaag
      541 atatcttttt cgtcgtattc accagttaaa taagcagata caggcaatac agcttgttcg
      601 ttattcaaga tagcttttac gatacgtgca gtactcatac caataccata gtaagttgct
      661 tttttacgat caatgatctc ataagcagca ttacggactt tatcagcaat cacatctaat
      721 tcatcttggg caatacgatg gtctttttct acaatttcaa acactggttt accaccgaca
      781 gttgtatgtg accatgcagc aacttctgaa tcaccatgtt cacctaagat atagccgtgg
      841 acgctgcgtg ggtcaacttt taatttcaat gcgatttctt tacggaaacg agttgtatcc
      901 aatgttgttc cagtaccgat aacacgtgat gttggcagac cagattcgtt ccaagcgata
      961 tatgtcaaaa tatctactgg gtttgaagca acaacgataa taccgtcgaa tcctgatccc
     1021 atgatttctt ttacgatttg gcgcatgatt gacgcatttg ttttaactaa atctaaacga
     1081 gattgaccag gtttttggtt gataccagct gtgataacaa cgatatcagc atctttacat
     1141 tcttcatagc cacctgacca tacagccaca ttttcatcgc cccaagccat accatctaaa
     1201 aggtccaatg cttccccttc tgctttttct tgattcacat cgatcaatac taattcatta
     1261 gagattcctt ggttgatcat tgcataagcg atacttgttc caacaaaacc tgtaccgacg
     1321 attactactt tacgacttgt ttttttcatg aataaacagc tccttttcat aactatttgt
     1381 tactttcatg aaaagtatag tcttgtttat tcattttatc aacatgttaa ttataatagt
     1441 tgttattata atttttgatg gaaacgaatt cctgaatacg cataacagat aaatgacaaa
     1501 tgtttacacc tatatcccct gataatacaa ccaaaagttt agaactgaaa ataaatccaa
     1561 aaaaattcgc tgcaacgata taataagaca gcaatcgcac ctaaaacaat tttccttcat
     1621 atgctttagt gaaagatttt cttcttaatg tttatttctc cattcattgg gagtacatgt
     1681 tgaatacttc ttgaataatt tgaataaaga agtcgtttgt ttatatccga tacgattagc
     1741 gatttcttcc aacgaaaaac tcgtttcttg aatcagttcc aacgcaaagt cgaatttttt
     1801 ctttagaacc agttcttgaa aggaagcatc aaaatacatt ttcaatttat tactaagata
     1861 gaccggattg tagccaaaat gagctgctgt ttcttttaac gtacaagaag taaaatgctt
     1921 atcgatataa tctaatactc taagcatctc attttctgcg catccatctg ctgatgaaaa
     1981 gctttgttgt tctaataaat gagtcaattc gatcagtaat agtgatgtat agagatttaa
     2041 cgaacggatc ttatgactgt atgtatcaaa ataaatcaac agcatatttt caatcaaatg
     2101 cgtcaaacga cttgtttttc cgccgcgaaa atgaatgaat tgtgtatgtt cccctttttc
     2161 ttttgatgca tttatcaaaa aagaagaaag tagattattt gtgcttgcca aatgattcaa
     2221 tatctctgta gataaggttt cttcttttaa taagatgttg attaaaatat cctcttgccc
     2281 tgtgtattcg attctttgag tagcttcttt atcaatgatc accacgtcac tttcttccat
     2341 ctctatcctt tctccattaa taatttgtaa acatgtaccg ctatacatat aattgatttc
     2401 aacaaaatca tgtgtatgct cgggaacttc cgcatatcga cggtgtttac tgatatgaat
     2461 atttccctgt tgaaatagat cccctcggtg gatttttggc ggtgtctgaa gatggtaagc
     2521 taagtttaaa tcattgatat tcattccttg ttcacgctgc agagtttcga ttgattcttc
     2581 gttacgcaaa aaaaatgtct aattcagcta gttccaattg actccctcct ttttgtaagc
     2641 ggtttctttc gcttattagt tcacgctgta ctgctattat atgcaaagct atttttggtt
     2701 tcataaagac tttctatggt tgggacatga acttcctgtg attccgctat tgagagaata
     2761 accacttcat actatatttt tgataagcgc ttacaaaaat gaaaggaaca tgcttatgaa
     2821 taaaaagatg atgaaatttt ctctgctgtc agtctctctt attttgactt ctgctggtgc
     2881 agtcacgtct gcaattccca gtatgtcagc tacttatgcc acgcgttctt tatctgctgt
     2941 tgaattatta acgactgctc cctcttttat ggtcactatt tttgttttga ttagtagttt
     3001 tattgctagg aaaatcggac aaaaacaaac tgtgatcatc ggattagtga ttgcatcgat
     3061 ttgtggtatc ctccccctct tctcacaaag ttacagtatt gtcttagctt caagagctgg
     3121 actaggtatt ggctttggat tgatcaattc tttggcagtc agcatgattg ctgcctttta
     3181 tcacggagat gaacgagcct ctatgattgg ttttcaaagt gcatttcaag gatttggtgc
     3241 agctttaatg acatttgttg caggacagct agtacagttc ggttggcaat ataccttttt
     3301 agtctacgcc atcagcttac ctattcttgt cttgtttgtt ttgtttgttc ctacacctga
     3361 aacaactgaa cttcaatcgc atggcacagc cccaacaaga caagtaatca aaacgaatcc
     3421 taaaatgatt agttatggta tttttctctt gctggtcatc gtggtatata atgctgtatc
     3481 gattaaatta gccacagtcc tgacgactta ttctctagga aacgaaacta atgcagcgaa
     3541 tatcttaaca atcatgcaat tggcaagtat ggctgccgga gtttgttttg gctggcttca
     3601 gacaaaagca catcgctata tgcttacact ttctttacta ctgatgggaa tcggctttgt
     3661 attgattgcc actgtgataa acctgtatgt gattgggttt ggtgcgatcc taacaggaat
     3721 ttctttttcg ttattcattc cttacttatt caaccaagta agtatccaag cgcccaagta
     3781 cgcggagagt ttcaatactt caatcttatt ggttggcgct aatttaggta ctttcattgc
     3841 accatatggt ttgctctttc tctcttttgc ttctcttggg agtaaaacac ttgctgtttt
     3901 cgtcaatggc agtatcttgc tgttgattag cgcgcttatt agccttctag tagtacacag
     3961 gacgaataga aagacagtga taagagtaga tgaagaaata aaagaacaat tgacaacaaa
     4021 ataaatttga tggaggtcgt gtaaaatgac aaatgaaaaa cataaagata gatggctttg
     4081 gtatcctgga gactttgaga tccgccatgg actgctacaa aatttccaac gggaagaacg
     4141 aggttttgac tggccagcgt actggtacat ggatgattgc catcggaacg tcaagtttaa
     4201 gagatattac tttcttgatc aaccttccat gtttaaagtg acgatccaag gagttgggta
     4261 tgtagaaatt aatggacaaa aacatccatg tggaaaatgg ctgacttgtc cagctggtaa
     4321 agcaaaaatt cgtatctttg taggacatac atcagggcta ccagcgatgt ttatcgaggg
     4381 agacgaagta aaatcagata ttggctggac agcaagtaat tttattgaag aatatccagc
     4441 tggctggagt ccgctttatg tcgatatcgc aaaagatcca aatcaaatct attatcaaaa
     4501 agaatgcgtc catccagtca ctgaaacgga gattggcgga ggaatcttat tcgattttgg
     4561 gcgagcagtg aatggctgtg tagaactaat atctctggat gaccatatcg aatctatcac
     4621 tctatgttat ggagagtccg atgtagaagc tatggatatc gattattgct actataagca
     4681 aataggaatg acaagaaaaa tgaaagctcg taaacgcgct tttcgctatt tgtttatacc
     4741 agaggtttct actggaatgg ttcaacttca agtttttcat gaatttttat ctctttcgaa
     4801 acaagctgct ttttcaagcg attcttcttt actgaatcag atctggtcag tatcagaaga
     4861 agccttcaag ttatgtagtg atttgttttt tatcgacggt atcaaacgag atcgctggat
     4921 ttggtctgga gatgcgtatc aatcattcat gatcaatcaa tatctttttt ttgacgagga
     4981 aatcaataaa cgaacgatat tagcattaag aggacaaaat gaaatcaagc agcatatgaa
     5041 tactatcgtt gactattcga tcttgtggtt gattggtatc gaaaaccact atatgatgac
     5101 gaaagactat gaatttttac gcatcgtcta ccctaagatg caatcattaa tgagatactt
     5161 gatggagcaa acgaatgaat tagggtttat ttatgggaga gcaaaagatt ggatatttat
     5221 tgattggtct gaaatggata aagaaggaac gcttgctgca gaacaagtct tattactgaa
     5281 agcctatcat tccatgatca cttgcggaga tatcttagct gaaacatatg gagagaacga
     5341 aaattatcat actcatctct atcaagaaaa atatcaacta ttgaaacaaa acctatttga
     5401 ctacttttgg gatgaagaac ttggcgcttt tatcgattgc tatgaatctg ggagaagaaa
     5461 tgtgacgcgt catgcgaata ttttcgctgt tttattcgat tttgctaaca aagaccaaca
     5521 gcaatcgatc ttaaagaatg tcttattgaa tgacactatc acacagatta ctactccata
     5581 tttcaaattc tatgaacaag atgctttatg caaactcgga cagacgaata tcgtctacca
     5641 aacgattctt gattattggg gagggatgtt atctcatgat gcggtaactt tctgggaaga
     5701 atacgaccct actcaaacag gaaatgaaaa gtacagtatg tatggagatc cttatggaaa
     5761 aagcttatgc catgcatggg gagcaagccc aatctatctt ttaggacgct attttatcgg
     5821 tttgtatcca acaaagccag gttatgaaga atttatgata caacctaatc tagattcatt
     5881 tgaaacactg aattgtacgc tacctatcaa agatggaaag atttcgcttg aaaaaacaaa
     5941 agataagatc gtgattactt ctgatagaac aggcggaaca gtgaaaatga atggtaaaag
     6001 ttatcctctt ctagcaaaac agccacttgt cttaacaagc tattcaaaaa taaatgcata
     6061 aaacaaaaaa ctgcttgcga catatcagaa gactttaaat cttcttctga tattgtccaa
     6121 gcaattttta ttttgaagtt tgcttaattc tgttctttag aaacaatgtt tgttcttttt
     6181 ccggcaaaaa gaaagctaga taatacgaca agtaaaaaga aagcgatgat tccaactgtc
     6241 aaggctattt gaccgtattc tcctagtttc ccaagtacaa gtcctggtac tagataatca
     6301 gtatcggcaa atgtagaaga agcgatttta agatctccta atactggaag gataaataat
     6361 ggcagccaac taatcaataa gctattgaaa gcagaaccta ataccgcccc tcttctaccg
     6421 ccagtcgagt tgccgaagac accagaagca gctccgcaga agaaatgtcc tactacccca
     6481 ggaatgataa ctgtagtttg caagcctatc atagcaaaca tggatagtgt tcctacaaag
     6541 aagctaacaa aaaagccaat taatacagca ttaggtgcat atggaaaaac gataggaaca
     6601 tctaatgctg gtttagaatc gggtacgagt tttttagcaa tcccttggaa tgctggaacg
     6661 atttctgcca acaccattcg aaccccttga aggacgataa caaacccagc tgtgaatgtt
     6721 ccagcttgga tcagtgcaaa gattacagcg ttgtctccat tgcttagttc tctttcaaca
     6781 aatccaggac cagctaacaa agcaagtgcg acatatacga tcgacatcaa gagcatgatc
     6841 gatacagtag aatctcgcaa aaagctgaaa cttttaggaa tctttatgtc ctctgtagaa
     6901 cggtctttgt ttccaaaaaa ctttccgatc catccactag aagcataacc gatatttccg
     6961 gtatgcccta acgcaacttg attgtttcca gtgatgttct tcataaacgg ttgacaaaga
     7021 gcaggcgtaa tagttaatag tgttccttcg aagaccccgc ctaataagat catccacaaa
     7081 tttgcagtaa accccgcgct tactaaaatg actgctgtta gacaagaaac ataaagcatt
     7141 gcttgacctg taagaaaaat gaatttatat cttgtgaatc tagcaagtaa aatattcacg
     7201 atcatcccta ctagcatgat caaagcagta ggtgtaccgt attgttgcaa agcaatcgcc
     7261 actactgctt cattgctagg aactactcct tgagtatgca gagctgtttg gaacatcgaa
     7321 gcaaaagggg ttagtgaatt actgattaaa ccagcaccgc cgatcagtac taggaatccg
     7381 acaaatgttt tgatagttcc ttggatcact tctgaaaatt tcttcttctg aagaacgaga
     7441 ccaagcagtg caatcaaaga aacgaggatg gagggctgac tgacgattga cacaaacaaa
     7501 ttcaacaatt tatccattga tagttcctcc cttgaatcat ttttattctt ttgacaagat
     7561 cttcgttact ttttctttta cttcttcgct gtcaatgatc gattgcaaag gtacgagctt
     7621 ttcatcaggt atagaagtta gcgtatcttt caaagaatgc tcaatgaaga aataatcagc
     7681 catatcgcta gatacacttc caacatccgt atgacttact tcaaactcgt ccacatctgc
     7741 tccaagatct gcaagtgctt gtttgatgtt catctctacc ataaaacttg ttcctaatcc
     7801 actttgacat actgcaaaaa ttttcatatc attcgtctcc ttttcttatg atcgctaata
     7861 tttcttcttt tgttgatgcg tgtaataaat cttgaagatt ttgtttatta gaaagtagct
     7921 ttgttaaatt tgccaaagcc cgaagatgtg cctcattatc gatagcagct aaacagatga
     7981 acaaactaat gggatgggct ggattatcaa gcagatccac aggttgttga agtttcagca
     8041 aagacatccc tacttcgttt acgccatctt ctggacgagc atgaggtaat gcgatgtgat
     8101 cgccaatatg gataaacgca ccatactgct ccacgcgctc gatcatagct ttgatataac
     8161 tttcttctat tttattttct gataatagcg gctgtgcggc aaatgtgatt gcttcttgcc
     8221 agttttttgg ttgatccgat aattggatca tttcttctgt taataattca gatagcatag
     8281 gacgttgatc ctccttcttt tctaaaagtt tgttcatttt acgatttaat acttgataga
     8341 gcttttcttc gtctacgcct tcttttaatt cgatataagg cagtagcgcc gacaacaatt
     8401 cttgggcaga aggcattgaa aatcctggta tcagccaatc tctttgtact tgattgatca
     8461 gctgattttt ttccaagtca gtcatgattg gatgcagaac atagagagac tttttcttat
     8521 cagtaagtgt gagaggtaca gtagaaaaaa taatatcgta agaagtttcg tcaacctcgt
     8581 gtaagcggtc agctgaatta gtctctgtaa aaaagatcat cgggaataat tgctgaagtt
     8641 cactttttaa gatcaatgaa gagctaatgc cacttggaca gacgatcacg gctcttattt
     8701 ttctatttct ttcttctgcg tccgcttttc tgatctctcc gccaaatagt atagtgaaaa
     8761 aaccaatctc ttcttcaggg atgctcttcc ctattcgtct ttctaacgga agtaatgctt
     8821 ttcttgtcat ttcgtagatg gaagcatatt gatgcttaat ttgatcgatc atcacattag
     8881 gtaaataaaa agaataggaa atacgaaaat aggcagggac caagtgataa aagacattca
     8941 tcaataattc acgaaacgaa gcaaattcta cggccatcag gaattccatg cgatggatga
     9001 cttctgaaga acattccagc aaaaaatcaa gtgcaggctc ctggacatct ccttcaacga
     9061 tcgatgctaa gcaaagagag aaaaatataa tctccgaatt ttgatgttcg gaatagccaa
     9121 ggccagtcaa tacttgtcga ctaatgtcag tgactgcaag agaagccaac agttcagcat
     9181 cttttaccag ttccatggat gtttttccaa ttcgaatggt tgtcaaactc aaataataag
     9241 ctaattcatc cattcgattt ggtgcgaatc gaagatctga tagtgaaatc gttttatcta
     9301 gcaaatctct aactttggcg taataatcaa tggaaagggt tgcggttaaa tacgtgagcc
     9361 caaatcttcc agcaagtgac tgcatcaaac gagcagtgcg attttttgct tctcttctta
     9421 atattgcttc tgaccctagc agcctaaaac cagttttcct agtatacgac agctgacaat
     9481 cttccacttc taattcattt cttatctttt tgatatctga caggactgtc cctttagaaa
     9541 cctgaagaaa atcttgaaaa tgaaaaacag aaacagacgt tgcagtcgaa taaaccatca
     9601 aataaagaag atctcttcgc tcttgttcgg tatacacagc ttcaaatcga gtaatatcca
     9661 aaaatttttc tgcccagtcg attgctcgga atttctttat acggatacgg gtatcatcca
     9721 catattcggc aaaaggagtt aattgtttga tcgcctcgct tgtatttact tcgatgatct
     9781 ctgctgcttt ttctagactg atttctcttg gtgttttcag gagataacta attttgtagt
     9841 tcacagcgct gttcacctcc tacttatatt ctagtatggt agcgctttta aatcgagggg
     9901 tgtttggtac aacttacagt tggatttttt ttacgactgt accgaacgat ttctatcgaa
     9961 tggatacaga aaaaaagcag gtgaccaaat gtctcctgct tttatgaatt gatttcgtcc
    10021 gatcaatgat gatcatgttt attttttgag gtcagttctt caaatttttc tttgacatct
    10081 tcaaccgctt tttcgatatc tttttcaaag tgattgggct cattaggctt tgtttcctct
    10141 tctacagaat ttgaaccaga taaagctgtt tctttatttt ctgtttcacc agagtttgtg
    10201 ccagctaatg attcttcttt gtcttctgtt tcaccagaat tagttcctgc taaagccact
    10261 tcttcttctg ttttaggggc cattgaacct cttaatcgtt tgtgtttttc accgtcataa
    10321 tttgtcatgt tggttcctcc tagtagttct atactttttc atcgtacaat aagacagttt
    10381 atgcgtcaaa aaacaaaccc gattcttaca aatatgtcat atttttcctt ctgattcttg
    10441 aatgcttttg attccttctt ccaaaatttt catttgtaag attgtttctt catctttgat
    10501 gactttttct gcgccattaa ctaatgtctc gtaaatccct tcgtagacac ggctgtaatc
    10561 accaacctcg gaaattactt tttcttcatg ataactccct tgttcatcta tataggtcaa
    10621 tacaccataa tgttcgggca aatcgatacc aaagtcttca tgatcaggca tgtaaaacat
    10681 tttcagatgt tcttcttgtc gatcttttgt ttgtttcacg aacatccctt tttttccgta
    10741 aacgacgaaa cttggacgtt ctttgatgcg gaaataactg gattttatcg aaattttcat
    10801 atttccatag aaaaaatcca gatcgaagta atcattcatt cgtcctttcc ctaaaagttg
    10861 acgtacatca tagctgatat catctggttc gccgaaataa gaaagaactt gatctactgt
    10921 atgacaagca tgtccatata aatagctgtt cgcagtagaa aattcatgcg tattttctgg
    10981 tacttccggt ctgaaataat cataatgcat ttctacttct aaaatatccc ctaattttcc
    11041 gctttcgatt actttttgga ctgtcaagaa gtcagaatcg aaccgtctgt tttggtaaca
    11101 ttggataaat agaccttttt cttttgctaa tgcaaataac tcttctgctt cctttgacgt
    11161 ttctgcaaaa ggtttctcta ctagtacatt cttaccatgt tcgagtgcca tttttccata
    11221 ttcataatga aattgactag gtgtagtgat gacaactaat tggatctcag gatcatccca
    11281 aagatcagac agctgatcag tatattcgat gtcttcgaat gttttccaca ctggttcaga
    11341 tggggaaaaa atttttttga tattgatttt gttcgtaagt tttcttgaaa aaggcaaatg
    11401 atagcgattt gtactctttc cattgccaag ataagcgatt gtaagcatga gtggtgcctc
    11461 cttttattga tgttttcagt atatcctaga aaagcgcaga aagtatttat aatagctatt
    11521 ctggtaaaaa agttcagaaa aacacttgtt atcattcggt ttggtgaaat taatgttcaa
    11581 agtaaggata aaaatcaaaa agatagtagc ggaaccaagt gtttatcagt aagatagaat
    11641 ttggaggtca gatcatgctt attgaagaaa aactaacaaa acaagagttg tttaccacga
    11701 cagagaaacg gattgtcgat tacatccgta ggaatatcga agctgcagtc tacatgacga
    11761 ttgaagaatt agcaaaagct acgtatactt cccattcagc tatcattcgt ttatgcaaaa
    11821 aaatgggatt cagcggattt aaagaattcc gtttcgagct ggggcgggaa gttcatcaat
    11881 tgattgctca gttcaatcaa acgattgatc ctaattttcc tttttcaagt gaagatagca
    11941 ctcagcaaat tgcaaagaaa atggcggatt tatcgatcca gtcaataaaa aaagcacaat
    12001 tgcagattga acatgaagat ttagatcaaa tcggtaaagt attgaacaag gcacagcgaa
    12061 tctttctttt tgcaaaaggt gattctcaaa tcacggcaag gaaattccag aataaaatgg
    12121 tcaagttgaa taagtttttg atcatggctg aagaatacag tgattcttct tggaatgcag
    12181 ccaatttgac tagcgaggat tgcgccattt ttattagcta cagcggtcgc atccatcatt
    12241 atgaacgaat aatgacctat ttgaagcatg tagaggcccc taccttgctg attaccggaa
    12301 atcagcattc agaaatggcc aaacaggctg atatgtgtct tgttatatcg caggaggaat
    12361 atgattttgc taaagtagcg actttctcct ctcaaattgc cttcgattac gtattaaata
    12421 cgttattctc agtgatatat tctcaaaatt ttgaagagaa tatccgcaac ttgaaaaaca
    12481 aacaagaatt gatgcaaaaa ggccttctta aagaatatcg gaaaacttaa gaaaagatcc
    12541 tcatttagta ataagaaaga tagtgtttga taggctctct ttgatatcat cataaaaaca
    12601 ggctgaagtt ttctttttca aacctcagcc tgtttgttta cggaacgatt cgaatagata
    12661 tattatagaa atgaggaata gctaacttct gtttcttatt ttgaacgatt gacgaaaaag
    12721 atatataaga aggaggaaaa tcagtataca tgcagctaac aatccataca ggaaaaatcg
    12781 tttgttctcg gatactttct gtacttcttt ttgaacttct tttgcatgat aaggaacccg
    12841 gtgccctctg acaagtaagc gatgactgtt gaccatataa ggtgtacaag ttaggagtgt
    12901 cacgaaatcc tgcccttttt cgattctcag atcttttgta tcagtcggtt ctaccgtctt
    12961 gatctgatcg acttgataag cgagtgtccg tccattgatt tcgatataga aagaatcacc
    13021 taaagtcagt ttcggtaagt ctgtgaacaa ctttgcttga ggcagtcctc gatggctgga
    13081 aataacggca tgtgtatttt ctccccccgt aggataagac gttccttcca ataatgaggc
    13141 acctttttct agcagcacag tattcgtttc atcaaaaatt ggcaaatgga cattgatctc
    13201 agggatcgtc aaaataccga tcgtatgttt ctcaaaatag gttctatcct ttttcgcagg
    13261 ttcttttttc ttagtaaagg gatctgcacc tggatttcca ccttcttttg ctaattgctg
    13321 gtttttcttt gtcatgtttt cttggatttt agccatgact tcttcatttt ctttgaccgc
    13381 ttgttgctga taatgagaga tgatctgctg atccagataa ttattcagcg cgtcgctaac
    13441 aaatggataa gctagtatcc ctattcctga gagaagcaag aaaagcatca gcagatctag
    13501 aagtttcctt ttctttggtt tcatttgtgt tctccttgtg atgactgttt gataataaaa
    13561 gaaaaaggag catttgcgta atccgctgct cctttttcca attcttgcaa aaagactaaa
    13621 tctgactgtg cttgcgtcta gcaaagtaca gtccagcaat cagtagaagg actactcctg
    13681 caccgatata cacatagatt cccttaccgc ctgttgaagg aagtgtacct ttgtgtttat
    13741 ttggtatttt ttcaggagaa atcaactgtc ctgctgttac ataagattgt tcatcgatag
    13801 taaatgctac acggtttgtt aatggaacat atttttctgg cgctttcgtt tcttccagat
    13861 agtacgtgcc atattttaga cctgtcacat cgattaaacc gttacttgtg gttgtaaaaa
    13921 ccgttgctga ttctttcgcc gatacccagc tgacggcttt tgtggatggg tcgatcgata
    13981 aatatttcgc tgtgtcacta tcttgatcac gaacgacgaa ttctgctcca gcaagtgttt
    14041 ggtctgatgt aacgtcacca tctactttaa caaatcgttt gcccccagta acgacatcga
    14101 ctgacggtgg tgtttgatca tttgtatgcc cgttatcgac atttgcttgg ttagaaaatc
    14161 ctttggttgg atctgctttt tcgttcaaat gcatatagta aacgaatttc aatgtaccgc
    14221 caggagttaa tgaaggaata taattcggat caactgaaac cgtgaatccg tctgtttgct
    14281 cagtgacaga ataatttact gggtcgattt ctttatttcc atcatataag gcataagcat
    14341 acgttcctga agaatcatta tcaaatgtta aagcagcgtc atgagtatcg ataagtttga
    14401 atgttgtgta cttgttttgc gttccttctt tatcagcgat acctaatggg atattgacag
    14461 aaatctcata ttgaattttt tcaccgattg cgacatcttt tccattcaat tgaggtgttg
    14521 ttttatcaac tttagacgta tcatttttga tggtcttttc tactggtgtt tggctatttg
    14581 ccaggatctc aaaaggcgtg attgtttgct tttcaatcat ttccgcatta tcaggagctt
    14641 ttacttcttc taaattatat tttccaactt ctaaatgatt aacgatcaac tggccttttt
    14701 caatagatgc ctcggcaaag tcattgttgc cgatgtcata agaatgacca gtaatgaaat
    14761 gtttggcttt ggtttgatca gttgtccaag tgtacaatcc atctgtgaca ctttggatgt
    14821 attttttgac gcttggtgtt ccttcttctt tagaaataat aaattctgct ccatttagtg
    14881 cttcgttttc ggcagtaccg atttttgtaa ctttcaacgt tccatcatta ccgactgtat
    14941 ttttagggta gagatggata gtatctagtt cttctgtccc gtatttataa gagccatctg
    15001 cttgtttgat catctcatat acagggaaag ctaaaaccat gtttgcggca gctgacactc
    15061 cgtcttttgg ttcttctttg atcgtgtaga ctgcatcttt cccattttgt tttttaggta
    15121 aagataaagt gacatttcca tcagcatctg tcgttcctga agcaactggt gtaccaggag
    15181 tcaaagactg gactgcttgt tttgctgcat ccacggacgc tcctttatct cgttgcgcat
    15241 aaaattcttg agtgacgtta taaactgaaa atgaaatatc ggctaatcct tggtattgat
    15301 cgaattcgct catttctttc ccgctgtttt ggattaaagg atcgggtaaa tcagtcattt
    15361 tctttttatg aaggatgact tgagctgctg tctcctctgc aaacactttt tttgcgccga
    15421 agctgtttgt gagtaatggt aaaataagga aaaggactcc taacataacg tttatttttt
    15481 tatgattttt cattttttca ctcctgttct ttttttgata aacaaccaga ttgcaaagct
    15541 tgcgaatacc ataccaacaa cgcctaaaag tgtatttttc gtttcgtttg tttttggaag
    15601 aaatggacgg ctattttctt ttttattgac tactttcaat atactgtccg cctctttatc
    15661 ctcagaaaga aaagttgaaa tggtaaacgg aatttctgtt tcacttaaaa tataaccaga
    15721 aggtgcttcg atctctttta atcgataact tcctgttttc aacccggaaa ttgaaaaagc
    15781 gccattttta tcggaaatca ggtgaagcgc atctgattcg ttctttgtcc acttgtatcc
    15841 gttggctgtt tgctggaggt aatttcctgc tttgttttta ataaggaata ctgcgccagc
    15901 taatcgttta tctggtttat ttgtctcgat tttgacaaat tgcttactcc ctgtatatac
    15961 agagactttc tttgtactga caccaaaacc aaaatccagt gttccgttgt tgttgatttc
    16021 cttgttcgcc tgtgctgtac tgcttagctg catctgataa gaaatggtca gtttttttcc
    16081 agcatatttt tccaacgaag ggatcgaaaa taaaacatca aatccgtgag gtttcttgtg
    16141 tatgacaaag ccttctgtca gcttttctcc attcgatgaa atcgttaaac tattaggtaa
    16201 aaacgttaat gcttcatccg cactatctga caattcgaac ttttgatagt caaggatatt
    16261 tacaggaatt gtcgtatgca aggaataagt gattttttca ccaatcgtaa aatcatttct
    16321 aggctcttct aataccttct caaaaggtgg atcagggtag tcgttttctt catttttagg
    16381 atacaaatgg atagttgaaa gtttttgatt gttttgtcca tgtacaggaa gaacaactac
    16441 catattctct gccttttctt tgacgacttc tggtactttg gattcaatga aaagatacgc
    16501 tttatctctt ttttcttgat ctttagcggc cagagaaaaa gaagcgatac catcttcgtt
    16561 gttaagagtt gtagttgttg cttctgcggt aaacataccg gaagacgcac cgttttttgc
    16621 gatctctgct tgtgcttctt ctaccgtttt gcccttttcc cgtagatggt aaaaagaatc
    16681 tgtgacatca taaacttgga atgtgacacc atttaatcct cgatacgttt gtaataaagc
    16741 tttttcttgt ccgtcatttg gatgattttt cggcagttgc ccgtttggga aaagcaattt
    16801 gtgtaaagta atctgaacca tttctgtttc tgtttctgtt gctacctgag taaaagctgg
    16861 tttaaatagt aacaagaaga gacacatact aagccaacca agttttttca tatttagctg
    16921 ccccctcttt cttttttcaa aaaaagatac caaatagaaa acgcacaacc aatcatccct
    16981 actagataga ttcctaaacg gccaattcct cccgtttcag gtaatggtac ttttgcctga
    17041 ttcgtgatgt ctaaagaaat ctggttgttt ttggctcctg gtgacagaac agattcatga
    17101 tcttgtccat ccacttgaat tgacccatct tcgtgtataa ctatagtaac tggctctttt
    17161 agaccttggt atccttctgg tgtaaaagtt tcggtcagcg tataagttcc aggttttaaa
    17221 ttttcgaata gaaaggtatc tgtttcttgt ccatcttttg gcgattctaa gtccatttct
    17281 ggtccctgca gtctgaattt tgctcctttt aatgtctgtc cgttgtcttc ttttttgtgt
    17341 accgttagat caaacgcttt tagtcgattt tgatgagtga gcgtccatac attatttgtc
    17401 atctcggtat tttttcccgt gtaaatgatt tttccatatt tatcgatttg gaattcataa
    17461 tttcccggcg aagtatcata tccggcagga ccagctgttt caacgactcg atatttacca
    17521 ggtgtgagtg aatcaaaact ggcaagacct ttttcattcg ttgtttggct gtcaattggc
    17581 tgataagtac catttgcttc ttttttctgt agggaaaatg ctgctccttt aagatttatc
    17641 tctttgtccg tcccttgcat cgcatactta cgtactgcta caggaacttc aacaaaagga
    17701 ttttcaatag tcaactggat cgtatcatcg gaagtagtga ctgtttttcc atcaatggtt
    17761 accgtaccat cagaagcgat cttgatttcc caagtagtct ttttgcttaa tccatgccct
    17821 tctggagctt ttgtttctgt cagcgtatag gtcatttctt tttgcaattt gacattttct
    17881 ggaagagaat aggttccgtc gccatgatct gttagtaaag tatcaataga atctcctgtt
    17941 aatttgaaaa cagcgccaat aagatccttt tcacctgtag aggaattttt cgttattttc
    18001 aagtttaacg gtgtgaattg tttagtattt ttccatgtca ttgcatctat ttgttgagaa
    18061 tcgtatcctg gtacaggtaa ttctttgatt gtttggtaac tgaatgcttg accttgatta
    18121 ttgtattgag gtagtgataa tatctcttga taactttctc cgctatttgc agataatttg
    18181 tcaatgtctg cacgttccca cgtatttgtt gtatctttag ctggtttaat gattcgaata
    18241 tatccgtttt tccaagctgc agcatttgtc gtatgttccc gttgaatctc aaaagtaact
    18301 tgatctggac gatcagctag attgttgtca aattcttccc ataacttttt gatgtgaaga
    18361 ctgactccgg gagctttagc agatggtata ccgaattcag ctaattcatt ggtatcaatg
    18421 tttggctgga aagttgtcct gccgttcatt tgataccaga aatttggatg gaagtcctca
    18481 ttttctgttt ggattctcac ttggtaatgg atttggattt cttggtcttt tcctaaatag
    18541 atctgattgc tcttgatggt atttccttct atggaaatag atggagtgac cgttgtagga
    18601 cttgtcccca cactcttgac tgaaagtgta ccaggctgat aaatgaatgg ttctgcgatt
    18661 ggatcattta tttgtccatt gcttacagtt gctgagatct gtacagcttt tttggctagg
    18721 tattcagaga tatctgttgc atgatcagct gattcatagt aaagatcgcc tttttcatct
    18781 gatgaaacca tttgtcgcat acgagactct acttctgctt ttgagagacc agctgccgga
    18841 tcgctttgaa gttggatgcc aagaccatgt atttcgattc ctcgttcttt gagtgccatc
    18901 gcttctccga tggttgcgat aaacgtactg tcgattcttc tggatagatt gttttggtca
    18961 ggtgcatcat agattcttga gattagagaa gtatttcccg gccgatcttg cgtattagaa
    19021 aactgagttc cgtaataatt gctgcttgat tgtgcgtgta cccgctgtac tttataggaa
    19081 aatgttggta caccatccgt cagcaaaacg atcacttttt tatgtccatt tggaacggat
    19141 agcatgcttc ctgcatctct tagtgctttt tgtgtaaaag taccaccatt tgtccgtgaa
    19201 ggtgtaatgg attttacttg atttttcact gaatcaaatg aacccatctg tactgcaccg
    19261 ttactgtagc tatatccttc gcttgagtag ccgacatatc ccatattgat tttgtctgtg
    19321 ataccgctat ctgctaaagt atcgacaaaa cgatcgacac caatctttac ttcaccgatc
    19381 cgattattgt cgttcatact tcctgaccag tctacgacca atacgagatc aagaggcgtg
    19441 atatcttttt gtacatttcc acgagcattc aaataaacat caaacaatcc gggtgtactt
    19501 gtttcctttg catactttcg tatcgcatag tctgcttgat taccggttcc tccatattcg
    19561 atatacgaat gggtccgatc attaggatct ccattccaag aatttatacc atcccattga
    19621 tttgttccgt ttttattgcc ttgatgatca agaacatttg tatttcctgt aggttgccaa
    19681 ctggctttag gataagtgcc ctgatcatct gttgtatatt ctgtaggaat cgtttgatta
    19741 gatggcacac tcacagctgg caaggataat gtaggcaatg taaatgggct gacagaattt
    19801 tcgttttctt ttgatgattc ttcttctatt tcgtctggct ttggtactga cacatctgta
    19861 tctttcttat tttctgtctc atcaataatg tcatattctt tattatcgac gatcagcttg
    19921 tttgcctggc tgtggtaaag tgctggatcg attggaaaac tcagatcgat agtttgttta
    19981 gtgtttgatg agatttccaa cgtaattttc ccgctattta cctgataagc atagccatct
    20041 ccttcttttc tttccacatc acttaagtaa ttttcgacta gaccaatttc tttttctatc
    20101 ttttcagcac tggtgttatt cgcatttagc ttgatgttca gtgctttttt tccagactga
    20161 tccgaaatga cttcagctga aattgtttct aaaactgttg gatcatctgt agtgtctgct
    20221 attgcagata atggcacaaa gtttgataaa ctcaatatta tcagcatgaa aataactttc
    20281 agtttcttcc ctgtggtcgt catttttccc ctcctttttc tttttttctg ttttgacgtg
    20341 aatagtgaaa attattaaaa taaacaggcg ttacataatt ttatttattt taatatgata
    20401 attattatac gaagggcgct aaagggaatc acaccaattt tcataccaat tggtttcatt
    20461 tatgaagttg attccttcga ttaatgagcg tttcattgaa agaaaagggt tgagggagaa
    20521 aaacatatga tcaggttgtt ggaaggaaac tatagaaaga tggaaaaaat cattcgaatt
    20581 ctgtccattt ctgacaagtg gatgactaaa aaagaacttg ccaaacgaat tggtagttca
    20641 gaatctacat ttgtaaggta tatcgaagag atcaaaatcc gctggggaac ggatgtaacg
    20701 attcaatctt cacaaaaatt ggggtatcgt cttgaacatt tgaatgtacc agcttattta
    20761 aatgttctga tggaaatgtc gcaaagttct acaaacagtc gtttattaaa tgagattatt
    20821 gtcaatccgg gtaaaacgat tgagtattat tgtgaaaaaa tttccatcag ccggtcttct
    20881 tttacacgta agttgaaaca atgcaatcaa attttagaaa gctatttatt aaaagtgatc
    20941 gttgaccaag gttttcagtt aactagcctt aaaagtgagc tccagttgcg tatctttgtt
    21001 acctttttct ttttaagtta ctacggaaga cgcacgttgc catttccaat cgaaaaaaag
    21061 aagatcaaag aaattatggt caagaataat tgtcagatga atttgtttca ggaagaaagc
    21121 ccatatgagc aatctttctt tattatgtat tttgttgtgc atctcatgag agaatcccaa
    21181 ggaactccat ttgaatggaa agctggagac tcttatgtac agttgataaa tatcgataaa
    21241 aaagacttct taatcttgaa aaatctctta ggaaaattga agtacagcag ctatataaaa
    21301 gtagtgaact attttgtcaa tacgattttt tcagaatttt cggagaaaga aaaaaatgaa
    21361 atcagtgagc agatacgaaa aaatatgtcc tactgttcgt tttcatccgc tgattgcaaa
    21421 gaaaaaaacg aagaatatgt tgttcaatta ttaacgcaaa tttatttttt ctcccgtatt
    21481 ttcccatacg atacgactca tttgacgatc cgtgcaactt actttgcaca gcagatgcaa
    21541 caccgccaga tacgtctata tgctggattt cgtgcagaac ttcaatttta ttccactcta
    21601 ctgaatcaga atctgattta ttatttatct agctatatct tttggatgct tatcggtaat
    21661 cctgaaattc accattatac ttctgataaa aaaatactga ttatcagcga cttaagtctt
    21721 cgacatagtc aatatattga agaatatatc agtgatattc ttgctgtgca taaaattcac
    21781 tctgagacaa ctgctattac tgaagatcag ctgtcgaaat acaatcttct cgagtatgat
    21841 ctgatcgtta cgaatcaacc cattttgaat gcccatgttc ctcatatatt gattgatgat
    21901 agtgtgtcat tcgcaaatga agaagaactc atccgcttat tcgagttatg aataatcaaa
    21961 aaacagctat gtataaacca ccctgataaa aatttcagga caaatacaca tagctgtttc
    22021 tttttattgt attccttcag caatccaacg agatttatag cgaaagtctt ttaacaactc
    22081 ttcttttgtc caattatttg caatgagatc cgtcaaagtg ttcattacga gggtcatgga
    22141 aaagtatagg aaactaactg cttcttccga cgattgaatg gagaaatgat tgattcttaa
    22201 taattccagc catttttcgt tcattggaga atcagccgat agttctctac gtttggttgt
    22261 ctgctgatca tttcgctggg ttaacaagta taacctttgc caatagatac tttcgtgatc
    22321 taagtcgcag caccactcta aatatttttc gatccccaaa aagaaatgtt gtttgttttc
    22381 gctaatgaat tttaagatat cttgatggac tatgtgagaa ctgcttttga tcgtatacaa
    22441 ataagcatct tccaaatctt gaaaatattt ataaaaagca ccgcgcgaca tccccatctt
    22501 gtcaacgatt tctgagactt tgacttggct gatgtgctgt gagcaaaata tctccagtaa
    22561 taggtcatcg attctctgtt tctttgcgtt cgataaattg aaaaaagttt cttttggcat
    22621 ccacatctct cctttatctc tgattatacg tggatagccc ttcattgtca tgaaggattc
    22681 attccttctt tttctcttct tctgtcaagt acccatcttc cattcgataa acgcgatcac
    22741 tccattgaat catacggaca tcatgagtaa ccataatagt cgctttttgt ttctcatgag
    22801 cttctttaac tagtaatttg acgacttcat atgcatgatc tgtatccaaa ctagctgttg
    22861 gttcgtctgc taagatcaag cttggctcat tatataaagc gcgagcaatt gccactctct
    22921 gtctttctcc accagaaagg tctttaggat aactattttt aagtgactgg atatctaaag
    22981 attgcagcaa ggaatcgatt tttgttgaat cttctttttt tgaagtaact tggtcaacta
    23041 gttcaaattg ttcctttacc gttaaaaaag gaatcaaatt agagctttgg agaataaatc
    23101 ctatttcttt aaagcgtaaa tctgctcgtt ttttttctgg aagctcagtt agtgagtgac
    23161 cgttgatcac tatttctccg gaagtcggtg tttgcaatcc tcctgaaatg gtaaggaaag
    23221 tacttttacc tgaaccagaa ggaccaatga tgctgacgaa ttctccttcc tttacataaa
    23281 aatcaattgc tttcaaagct gagacctttg tatgaccaga accaaatgtt ttggtaactt
    23341 ttttcatcgt taatattttt ttcattttta ttaacctcct attgctgtaa ttgggtctac
    23401 tttggttatc gtacgtatcg aaaacaatcc gcctagcatt gcgacaatga tcaaaatccc
    23461 actatatagc aaccattgtg accaaaaaac agcaaaaggc atcgcgcttg gtaaaatcag
    23521 actagtcaaa taagcaaaca aaagagcaag cagaacgcct accaccccaa caataaagga
    23581 ctgtgctact aaagatttag cgatgaagaa atttttgatc ccttgtgctt tcattactcc
    23641 aaaaatagca gtcttctgaa gtgtcatcac gtacatgaaa ataccgacca cggcagcaac
    23701 cactaagaac aaaaagtata tcatcgcatt taaggtcata ttttgggcag aatagccagg
    23761 aatactttca atgaatgtcc caatagttaa taactgtaga cccttcgatg ctttttcttt
    23821 cgaaatttta gctgaatcct ttattacgat cccattgatc ggctgttctt cttcagaagc
    23881 aaacggctgt tgtccatact tcaaagcagt ccacgtatct agggacgcat agatgacggg
    23941 agaaactgtg taataagttt caggaaagat accgacaatc gtcaaaggat cgtcgtaact
    24001 gccaattttg atcgtatctc caattttata gcctgaatca gccagatttt gagagatgat
    24061 gatttcattc tttgttttaa attcatgccc tttcgtgagt tttggtaata aaaatgcttc
    24121 tttcgttgtg ccgaaaactg taacattttc tttgtttttt cctttgattg ctccgctgta
    24181 caatccgatt ggtgctttct ctcctccttc aatgtatttc agatctcctc ttgttaattg
    24241 tgaagctgca aatacttgat ttgcatcctc cgacaaaaca atctcttgtg cgtcccaatc
    24301 atcgattgct tttttaaact cttctgaaag tccattcgct agtcctgaca acatgaatac
    24361 gacatacgca atcaagaaca taataccgac gatcaagcca tatcgtaatt tagaatagcg
    24421 aatttctttg atagctagaa acatttgttt ttcctcccta tgatagattg atagtgacaa
    24481 aatgtcactt gttgaatagt gtaactctaa agtgacaatt tgtcacttgt gttgtttttt
    24541 tcttatcaat aaattgtcta agacatcttt cgataaaacc ttaacatgag tcataaaaaa
    24601 tttatttaag ccttatttat gctttccatg ataaactaaa gtgaccaaat caatgaggtg
    24661 ggaaaatgat taaactatta gtagtagaag acgataaagt attatccaat aatataaaag
    24721 aaatactcgc tgcaatcggc gagataacac aagtttatga tggtagtgaa ggactgttcg
    24781 agttggaaag tggagtttat gatcttgcaa tcttggattt gatgatgccg gaaatgaatg
    24841 gatatgaagt gttagcaaaa atacggaaag aaaaaataca gactccagta ttgattttga
    24901 ctgcaaaaga tagtttggaa gataagattg aaggatttga acgaggtgca gatgattatc
    24961 taacaaaacc tttctatcga gaagaactga tcatgcgcgt gaaagcatta ttgaaacgct
    25021 cgctaggtct ctttgatgaa aatgttctga agtataagga tatcagttgt gatttaaaaa
    25081 gcaatgaagt cttgtataaa gaacaagttt tgcccataca aggaaaagag tttgaattgc
    25141 tcgtttattt tttacaaaat aagggaatca ttttgacaaa agagcagata tttgaccgga
    25201 tctggggatt tgattctgat acgacgatta cagttattga agtttatatg agcaacttac
    25261 gaaaacatct gcgaagtact ggattggata aagaaatcaa aaccttacgt aatgtcggtt
    25321 atatactgca aagggtgtga tgcaatgaat ggagatatta cacgaaagca acgtagtcgc
    25381 ttttttgcga caaatgtagc agcatttgct tttatctttt ttcttttggg attcattact
    25441 ttacagatat taaaccaatc tgcatacaga gaaacagatc tgtcattaga gaaaataaat
    25501 tttgattctc gaacagtaca attagagata caaagatatc agcaaggcga tcccttttta
    25561 aacccgactc aaacggaaca aaacagaaca aacatggaac agccattaat ggaaccatca
    25621 aatgaccgtt ttaacactca agtgatcttg tggtctaaaa atggtgagat attgaataaa
    25681 gcagctattg gtgggcgttt caaccaaatc aaagaattaa aaatggatac gaaaaatctg
    25741 aataccatcc aaaatattga attaaatgaa gacagtggct catctgccct ctctttccgt
    25801 tcgattacga aaaaagcgga gacgaatcaa gatggcgttg cctacatcca aattttagaa
    25861 aatacaaatc aaatcaagaa ttcattagcg acttttcgca cgattatgat tttgtgtatg
    25921 attgtttttt ggattctctc tattggtatc agttattact tgtctagtct aagcatgcgc
    25981 cctattattc tttcttggag aagacaaaaa gaatttgttg agaatgcttc tcatgaattg
    26041 cgcacaccgt taacgattat tcaaaatagt ttggaacatc tttttaccaa gcctaatcat
    26101 actattattg aagaatccga aagcatcgct caagcattgt cagagacgag gcgcctcact
    26161 ggattgactt cggatttgct gacaatcgct cggaatgatt ctgatcagca gctgctttca
    26221 aagcaaatga tcaatacaca agaatatatc gaacaacttg ttaaaccatt tcaagagatg
    26281 gccatcatag atggaaaaga gtttattctc cataacgatg caacattaga agtaccaatt
    26341 gatgaaaaaa aaatccatca agttttggtt attttgctgg ataatgcatt aaaatatacg
    26401 aatccagaag ataagatcat tttggattca tctgttaacc ataaagaatg gctgattcac
    26461 gtaaagaata ctggcccttc tatcagtgaa gaagatcaag caaggatttt tgatcgattt
    26521 tatcgggaag atcaatctcg ggcgaaagag actggcggat acgggcttgg cttggcaatt
    26581 gcaaaacagg taatcgaaca gcatcatgga cagatttctg tatccaatta tcttccacaa
    26641 ggagtcgatt ttaaagtcca attgccgttg aagcctaaaa aataatgctt atccctaaaa
    26701 aagggctgtg acaaaaatct tgtcacagcc cttttttgtt gcgaataaac ggtgttccag
    26761 aagtagcttc ttcggaaata agccgaaatt tctttaagct gaccacttct gtcacaacct
    26821 ctttagctta cgcttacgat tttttattgc ggtcttcaat gtgtacatca ccgcttgtgt
    26881 caatatccaa ttcttctcgg ctaacatctt ctgtcacttt ttcaacatct tcatgttctt
    26941 ctttatgaat cccaacttct tcacgaataa ctgtatgttt gcttacatct acttgctctt
    27001 ctttgattgg gatagtaatc gtgtcatctt ccaatttttc gtctgttccc tgtgaagatt
    27061 gatctgttac tggtttgcgt tcgatgacga tttcttcacg tttgaccggt acttctaccg
    27121 tttcagtatc gttgaccaca tgtttgtgga tgtcgacttc tccagttgtc acatcatgtg
    27181 tatttacatc taatttttct tcttttagtt gaattgtatc atcatctaga tttggtttgc
    27241 gatcgatgtc ttcagtcttg cttgtaccca ttgtatcagt ctcgccaggt acgtccatcg
    27301 gatcagttcc tggtgtcata ttcaatggat ccgttgtatt ttgaaagcct gtcgtgccac
    27361 ctacgtccat tggatctgtt ccagtgccag tttctaatcc agtccccatt gtgcttgctg
    27421 ggctaacagt atcttgtgta tccattgtat cttctgttgt ttctggctga cgatagcctt
    27481 ttactgtgat gacaaatttt ccatcagaaa tatctcctct atattgatat aaaggatcat
    27541 tttctggtgt agccgtctca gaatcatagg tatcaaaaga aaaagtatct tttatttttt
    27601 cccataaaga acggtcttct tcaccatcca tccgattttc atctgtttca acatctatac
    27661 cagacaatcc gtcaaatcct agtctagatg ctgcttcatc agtagtataa ataacgatat
    27721 catctcgttc atacccttcg ttacgcaacc gttcaattac tgataatgct tcttccctcg
    27781 tactatagct tccgatgacc gttaaatttt tctcttccat gtaaattcct cctagcaaat
    27841 agatttatga ttggtatatt
//
LOCUS       DAZKKW010000029        27784 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.029, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000029 DAZKKW010000000
VERSION     DAZKKW010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27784
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.029"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            414..947
                     /locus_tag="ACG8PO_001238"
     CDS             414..947
                     /locus_tag="ACG8PO_001238"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFQ0206578.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
     gene            complement(1437..1961)
                     /locus_tag="ACG8PO_001239"
     CDS             complement(1437..1961)
                     /locus_tag="ACG8PO_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HFQ0206579.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(1954..2427)
                     /gene="tsaE"
                     /locus_tag="ACG8PO_001240"
     CDS             complement(1954..2427)
                     /gene="tsaE"
                     /locus_tag="ACG8PO_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFQ0206580.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(2550..3530)
                     /gene="pta"
                     /locus_tag="ACG8PO_001241"
     CDS             complement(2550..3530)
                     /gene="pta"
                     /locus_tag="ACG8PO_001241"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFQ0206581.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(3683..4363)
                     /locus_tag="ACG8PO_001242"
     CDS             complement(3683..4363)
                     /locus_tag="ACG8PO_001242"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFQ0206582.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            4489..5358
                     /locus_tag="ACG8PO_001243"
     CDS             4489..5358
                     /locus_tag="ACG8PO_001243"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFQ0206583.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            5384..5899
                     /locus_tag="ACG8PO_001244"
     CDS             5384..5899
                     /locus_tag="ACG8PO_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related/scramblase family protein"
                     /protein_id="HFQ0206584.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(5946..6509)
                     /locus_tag="ACG8PO_001245"
     CDS             complement(5946..6509)
                     /locus_tag="ACG8PO_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0206585.1"
                     /translation="MIRFARKEDAPEIAPLILVILKDMELPFLKQTGEQKTLEILEEA
                     ITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKYQLDASTRLFI
                     DKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLSVDEKNPKAKK
                     LYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(6564..6743)
                     /locus_tag="ACG8PO_001246"
     CDS             complement(6564..6743)
                     /locus_tag="ACG8PO_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0206586.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(6820..7329)
                     /locus_tag="ACG8PO_001247"
     CDS             complement(6820..7329)
                     /locus_tag="ACG8PO_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0206587.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(7425..7805)
                     /locus_tag="ACG8PO_001248"
     CDS             complement(7425..7805)
                     /locus_tag="ACG8PO_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFQ0206588.1"
                     /translation="MNLTTIHPIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            8135..8884
                     /gene="pnuC"
                     /locus_tag="ACG8PO_001249"
     CDS             8135..8884
                     /gene="pnuC"
                     /locus_tag="ACG8PO_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HFQ0206589.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKLTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(9068..10345)
                     /locus_tag="ACG8PO_001250"
     CDS             complement(9068..10345)
                     /locus_tag="ACG8PO_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0206590.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(10424..11068)
                     /gene="pcp"
                     /locus_tag="ACG8PO_001251"
     CDS             complement(10424..11068)
                     /gene="pcp"
                     /locus_tag="ACG8PO_001251"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016920 - pyroglutamyl-peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HFQ0206591.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(11267..12211)
                     /locus_tag="ACG8PO_001252"
     CDS             complement(11267..12211)
                     /locus_tag="ACG8PO_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HFQ0206592.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(12213..12899)
                     /locus_tag="ACG8PO_001253"
     CDS             complement(12213..12899)
                     /locus_tag="ACG8PO_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HFQ0206593.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            13372..13692
                     /locus_tag="ACG8PO_001254"
     CDS             13372..13692
                     /locus_tag="ACG8PO_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HFQ0206594.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGLLLISIIGIKI
                     TSGH"
     gene            13853..14758
                     /locus_tag="ACG8PO_001255"
     CDS             13853..14758
                     /locus_tag="ACG8PO_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0206595.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSEGTIRIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVKQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDQAARTILTDAGYPLEDPDKIKLLKKNEELLGTVIQLLSAENVHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            14755..15528
                     /locus_tag="ACG8PO_001256"
     CDS             14755..15528
                     /locus_tag="ACG8PO_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0206596.1"
                     /translation="MTLLIKQELFKLIKKKSTAVLSVLLVALLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMESQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSIYLYLLAIIVTVLMKLILFPSISFTEKISTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIALFLALGYLVFKRKNV"
     gene            complement(15705..17129)
                     /locus_tag="ACG8PO_001257"
     CDS             complement(15705..17129)
                     /locus_tag="ACG8PO_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid/polyamine antiporter"
                     /protein_id="HFQ0206597.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNVASIVMSTIFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFINNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGFLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            17340..17786
                     /gene="nrdI"
                     /locus_tag="ACG8PO_001258"
     CDS             17340..17786
                     /gene="nrdI"
                     /locus_tag="ACG8PO_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFQ0206598.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEYEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQRFGFPFLADYELRGTPSDVTRVYQILAEKNK"
     gene            complement(17856..19838)
                     /locus_tag="ACG8PO_001259"
     CDS             complement(17856..19838)
                     /locus_tag="ACG8PO_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose PTS transporter subunit IIA"
                     /protein_id="HFQ0206599.1"
                     /translation="MAKNELTLEELAKQIYQGAGGMGNVESIVHCMTRVRMKVINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQEMVDLAGVKLGEKLPSASATTAS
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAGTWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALATGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKAVPQASA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVISSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAADK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(19852..20748)
                     /gene="murQ"
                     /locus_tag="ACG8PO_001260"
     CDS             complement(19852..20748)
                     /gene="murQ"
                     /locus_tag="ACG8PO_001260"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFQ0206600.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELPSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISEHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEAAQAKLAEANGFVKGTL"
     gene            complement(20751..21812)
                     /locus_tag="ACG8PO_001261"
     CDS             complement(20751..21812)
                     /locus_tag="ACG8PO_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MupG family TIM beta-alpha barrel fold protein"
                     /protein_id="HFQ0206601.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQNRLIELGSEAKKNKLELMVDISGEALKRAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPNDIDELKEAAADFSQIEAWHNYYPRLETGLDKEWYQEK
                     NQWLKTQGFLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSEHVQEQFASFQKEQTVLLHTEPVDEVFYEYILGKHTNRQDDARDVIRSADARF
                     REIPPIPVRNTAKRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            complement(22024..22551)
                     /locus_tag="ACG8PO_001262"
     CDS             complement(22024..22551)
                     /locus_tag="ACG8PO_001262"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFQ0206602.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQALAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVVDRLNLPMTADERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECMEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELISYE"
     gene            complement(22618..24120)
                     /gene="eat(A)"
                     /locus_tag="ACG8PO_001263"
     CDS             complement(22618..24120)
                     /gene="eat(A)"
                     /locus_tag="ACG8PO_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection-like protein
                     Eat(A)"
                     /protein_id="HFQ0206603.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQAFVYFPQQTKDKERLTYYVLNEIMDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEIAKNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKKVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDALTMNSQVSHHKRLLSVENLQLGYENLLFEPVHF
                     TIEPHQRMAISGPNGAGKSSIIHYLLGTFNGKVVGEKSQPKHLTISYVRQNYEDNRGT
                     LAEFAKKNRVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYI
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLEKI"
     gene            complement(24378..25499)
                     /locus_tag="ACG8PO_001264"
     CDS             complement(24378..25499)
                     /locus_tag="ACG8PO_001264"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFQ0206604.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALGGTWRTIDGMDLLFRQRKISYLLTNWQGKNTAVIESAQVLGLDLLDKP
                     TDQTIRLASSYALGELIKDTLLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTRKLHLDRRNVLVSKVKVIIAADVTNPYTGQQGAAWQFGRQKGGTDTTLAFLD
                     KQAEKIAQQIKDVWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFELVSQITGLEKQI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDRELEGLFDFLPIV
                     LSIQLGPIDLEEAMEHRQTLEKTEILGQTLSRFFNILPK"
     gene            25625..26566
                     /locus_tag="ACG8PO_001265"
     CDS             25625..26566
                     /locus_tag="ACG8PO_001265"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFQ0206605.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVVLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNKHVDLIVALTKANQLDDMFRSIQSIITENTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEAKLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAEGM
                     VRTIVSEFASIAAKEGITLDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAVWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(26709..27605)
                     /locus_tag="ACG8PO_001266"
     CDS             complement(26709..27605)
                     /locus_tag="ACG8PO_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HFQ0206606.1"
                     /translation="MNKNDVLLIAKNKSLYFFPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMDFSSSEPKTDLFFGATNNGRKERAEKYKQEFTKR
                     GVTTNYFILPLRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYQFYHSENIFLLQERDLDELPEFLHTPFQPIPSELLEFFDAENWAKR
                     FFTTDSAIYEQYEYDLSSVKNK"
BASE COUNT         8056 a         5845 c         4937 g         8946 t
ORIGIN      
        1 cttattaccg cagatgcaag cttttgaaca ccgtttattg agaagttgtg aaatcagcgt
       61 cctgacctga gtattagaga aacaaatggc aaaatagttc ctcatatttt gctatttttt
      121 gagctaatac cgagggtcag ctgattgaac accgtttagt ggagaagttg tgaaaaagtc
      181 gcctactttc gagtcgtaag aaattttttt gaaaatggtt cctcacaact ttgcataaat
      241 ttgacttaat accgagaagt catgatcccc atctaactcc aaatcacaag gaatgagttc
      301 aaaaactagc ttttctcaac aaatattaca taaaaaattc tatgtttttt gttaaattta
      361 tttgcttttt tcaaaagtat gttacagtaa tttcccaagg aggttttaca gaaatgaatt
      421 ttgttgccat ggattttgaa actgccaatc atcagcctta tagtgcttgt tctcttgcgc
      481 tagtcatggt aaaaaatagc cagatcgttg atgaattcta tacattgata cagcctgaga
      541 ctcctttttt ctggcgaaat gtccaaattc acggaatcca tcaagaagac gtacgcaatg
      601 cgccgaaatt cccagaagtc tggcaaacta tccaaccata ttttcaaaaa aatcgtttag
      661 tcgttgcaca taatgctgct tttgatacaa aggtcttagc gggatgctta gattattatc
      721 atcttgaaca gcctaattat ctttctttat gtacagtgaa aagtagccgt cgcctgtttc
      781 ctgaatttcc aaatcatcga ttgaacactg tgtgcgaaaa tttgaacatc caattagatc
      841 atcaccatga tgcattggaa gatagtcgtg catgtgcgga aatcctgctt tatcaagaaa
      901 aaaaattcgg gacagatcca ttgaaaaaac ttgtgactat aaagtaaaag aatacttatg
      961 aaacacacaa agaggtcgtg agcctgccgt ttcactccga gtcacaagga gcaatttcaa
     1021 aaaatagctt ctcatgttta ttaaatattg tgactggtga ccgaggagct ggcaggagaa
     1081 caccgtttat tggagaggtt gtgaaaaagc tgttctgcat caagtaataa gaacagccaa
     1141 acaaaaatag cttctcatat ttttcatttg gttggactta ttaccgcaga tgcaagcttt
     1201 tggacaccgt ttaccaagag gttgtgaaat cagcgttttg acctgagtat tagagaaaca
     1261 aacggcaaaa tagttcctca tattttgccg ttttttgagc taataccgaa ggtcagctga
     1321 ttgaacaccg tttattcaaa aaaaatggac tgcgacacga cttttgtcac agtcccttcg
     1381 tgtgtttttc tgccttctca aaaggatgac agtttctcac tcttttccta tttcttctat
     1441 tcaatcaaat aactcatttt atatacatct aaaaattcac ctagatctgt tcttgcccct
     1501 ctaggcatga ccgcttcgat ttggaagccc atttttcggt aaaggtgtac ggctctttca
     1561 ttacgaactt gaacgtcaag ctccaatcga aaaagtacac ccatttcctt cgcccaactg
     1621 atgatttctt ctagcataag agtgcctagt cccattcccc aatattcctg cagaatactg
     1681 ataccgactt ctcccacgtg agacaaacga aattgactgt ctgccttgac agatgctgtt
     1741 ccaataatcg tcccttcata gattgcaagc agcaataaat tattattgct ttcgtatata
     1801 taatccaact cttcttctaa ggtttctggt ggtaacagga gttctgcttc atccaaaact
     1861 aaaaattctg tctccttatt aacttgtccc atcatcaaaa gaatgtcttt tgcatctcct
     1921 ggcactgctt cccgcaagat gatcccaact tcttcactca tgattttcag cccatttttc
     1981 tagaattcgc tgtttgaagg tttcactttg ctccccgtat gagattagtt tcacataccg
     2041 cttttctaaa tctgtatcac ttttttctaa aatcagttcc aaataatcct ccggcagtgc
     2101 ttcttctaat agatttcccc attcaacaac ggaaagccca tcaccttcaa aatattcatc
     2161 taaccctaaa tctgctccgc tagctgctac ccgataaata tccatatgat acaacggaat
     2221 ccgaccttga ctatattctc taataatcgt gtatgtcgga cttttgatca tttgcgttat
     2281 ccctaatcct tgggcaattc ctttcgtcaa cgttgtttta cccgcaccta gatcaccagt
     2341 caatatcaga ttgtcacctg gcattgcgga atgaccaatt gcctctgcga attgttctgt
     2401 cattgttaat cctgatagtt cgatcatttt tcatcttctc ccgctcttct ttctttttgc
     2461 cagtatatcg cgtaatgaag tttttttaaa gaaaaaatga actagtcaag aacagcagct
     2521 gctgtttttg gctagttcgc gtgtatttgt taatcaatca atgtttgtgc tgctgtgata
     2581 atcgataatt tatatacatc ttcttcattt gctccacgag acaaatcaga aaccggtttg
     2641 ttcaagcctt gtagaattgg tccgattgct tcaaagttac cgaaacgctg agcaatttta
     2701 tagccgatat ttcctgactg caattctgga aatacaaaga ctgttgcttg accagctact
     2761 tctgaatctg gcgctttcag ttgtgcaact gaaggaacat aagaagcatc aaattgcaat
     2821 tcgccgtcga tcatgtattg tggcgctagt tttttagcga ttttagttgc ttcaactact
     2881 ttgtctactt ctggcgattt cgctgagcct tttgtcgaga agctcatcat tgctactttt
     2941 ggttcgatat cgaacaattc tgctgttttt gcagaatcta cggcgatttc agccaattct
     3001 tgcgcagttg gattcacgtt gatcgcacaa tccgaaaaga catatttttc ttgatctctt
     3061 ccacgtacca tgatcatcgc tccgcttgtc cgactgatgc caggctttgt tttgatgatc
     3121 tgaagtgctg ggcgaacagt gtcgccagtc gaatggatcg ctccagagac cattccgtct
     3181 gcaatcccca tatacgtaag catcgtacca aagtaattca catcttttaa aatcttttct
     3241 gcttgttctt tggatgcctt tccatttcta cgttcaacaa aagtatccac catttcatcc
     3301 cacttatcgt aattatcagg gctgatgatc gtaaagctag atgttttgat tcctctggca
     3361 tgcgctgcat ttcttatttc ttcttcgttc cctactaaaa tgggttccac aagttcttct
     3421 gctttcaacc gggcagctgc gcctaaaata cgagcatcag tagcttccgg gaaaacgatt
     3481 ttgatcccac ggcgaacaat tttaaatctt aaactatcaa acaattccac aagatgacct
     3541 ccagacgttt ttatatgtat tatcatacac tattccagaa aaaaagaaca gtttatttta
     3601 tctgtatcag tcaaaaatgc tggaaaaatt aagtttttca catacaaagc gaactgcact
     3661 ataacaggaa agcgctgttt tattacactg tttcaggcag ttgccaatca atcggttctt
     3721 ctcctaacgc aaggagcgca tcattcgttt tcgagaaggg tttggaacca aaaaagccac
     3781 gacttgcaga taacggactg ggatgtggag atgttatgat gatatggcga ctcttatcga
     3841 tcattttgat tttttcttgt gccggttttc cccacaagat aaacacgatt ggtttttccc
     3901 gttcatttaa tttttcaatg atcgtgtctg ttaatctttc ccaacctttc ccgcgatgag
     3961 aatacgcttg cccttcccga acggtaagaa cggtgttgag taaaaggact ccctgtttag
     4021 cccatgacac tagatttcca tgttgaacag gtgcgattcc aagatcatca taaagctctt
     4081 tatagatatt tcttaacgat ggcgggatct ttacgccagg ttgcacagaa aaagataagc
     4141 catgagcttg attttctcca tgataaggat cttgtcctaa aatcaccact ttaacttctt
     4201 cataaggagt caattccaat gcttcataga tatgatacat atctggatgg atcttttggg
     4261 acgcatattc tttttttaaa aattctctta gatgcagata ataatctttt gaaaattctg
     4321 aggacaaaac ttcttgccaa ctgttatgaa tgatttcttt caaaaactcc acctcctaat
     4381 aaaaagaata actcacattt tcttcaatca caagggacaa tcttcaccct cagcacttga
     4441 ttaatatggt aaactaggaa caagaagcag aaaggaacga taatcattat gatcaaatta
     4501 attgcatcag atatggacgg cacattatta gatgcccata tgagtgtttc tcctgaaaat
     4561 gccgaggcga tacgttttgc aaatgatgcc ggtgttgagt tcatggtcgc tactggtcga
     4621 aactatcaag aagctcgcgc agctttagat gaggtaggaa tcgattgtgc gatgatcact
     4681 ttgaacgggg cacaggtatt tgataaagaa ggaaattcat tgtttaccgt gtcgattcca
     4741 aataatcaag caatctctgt tttagatatt ctagatgcta acggtattta ctatgaagta
     4801 gcaaccaacg atggtcttta ttctgaaaat caagcgaaac gaattgaaag ttttgcttcc
     4861 atggtggcta cccatttacc tcaccttacg tataaaatgg cgattgccat ggcctcagct
     4921 aagctggagt tattgcacat cacatacgtg gacagtattc gtgctatttt ggaagaaaag
     4981 aatctggaag ttttaaaaat tatttgtttt catacagaag ggccagctgt attaggtcca
     5041 gttggaaagc agatttctct tttaggcgat ctagccgtta cttcctctgg tcaaaacaat
     5101 atcgaagtga accataaaaa cgctcaaaaa ggaattgctg ttgcccatgt cgctcacgag
     5161 cgtgggattt ctttagaaga agtcatgacg attggcgata acttcaatga tgtcagcatg
     5221 ctgcaagtag caggagtcag tttcgcaatg ggaaatgcag aaatcgaagt aaaagattat
     5281 gccaaatata caacggacac aaaccttgaa tctggcgtag gaaaagccat attgcgcgcg
     5341 attgcagaag acttgtagtt ttatctagaa aagagggata tagatgtcag aatttttcat
     5401 tcaggaaaag cagcttggac gtgcaactcg gacaattgtc aaaaacgaaa aaggacattc
     5461 cctgtttttg atggttggtc gctggggaac aaagggcgat gcattgtctc tttatgccat
     5521 gaatggcgat ctcgttgctc acatcaagca gataagcctg acattcggta cgcgttttga
     5581 gctgtataaa gagttcgaaa aggtgggaac catgcagaaa atattcaatt ggcccggtga
     5641 cttttattat attcgtagac ttcactggac agtccaaggt gatatctata atcatcaata
     5701 tcaaatccat catttcaatc agcaaatcat gaaaatggat aaagcaacac ttttttcagg
     5761 agattactat gtattggatg tctcaaatga tgaagatgct ccagtttgta tttgtattgc
     5821 tgcggtttta gattactggc tctaccgtaa agacaaaagt cgcgataagc ctgtttctta
     5881 ccgacttaac ttaagttaaa aagatgtacc tgaaatgcgg aaatacgcat ttcaggtaca
     5941 tctttttaga tattcttttg catatgatca taaagatgac cactgatcat ccgctgaccg
     6001 acaactttga agtcatggcg ttcgtataat tttttagcct ttggattttt ttcatccaca
     6061 cttagaccga tgacggagcg attagctttc ttcgccaatt taggtaatgc ttctaataat
     6121 cgcgaaccga tgccttgtcc tctaaaatct tctgatacac tgatagagtc caaataccat
     6181 tcgttgggaa acgtttcttt atcaatgaac aaacgtgtag acgcatctag ctgatatttc
     6241 tctaagatag gtaccagtgg ctgatcgatg attgattctt cttcatctgt ataaccgtaa
     6301 gcaactcctg caactttacc gtctatttct tctacgatac ctcgtgaata actgtaacga
     6361 taatttggat cagtgattgc ttcttctaaa atctctaaag tcttttgttc ccctgtctgc
     6421 ttcaaaaatg gcagttccat atcttttaga atcactaaga tcaatggggc gatttctgga
     6481 gcatcttctt ttctcgcaaa acgaatcata gtttcctctc cttttctcca ttctaacatg
     6541 cagaaaaaga aatcgcaaaa tcgttatttt aaaaaatctt caatcttttc agtaatgaaa
     6601 taaccatgag taacgattct tgccgcttca gttcggcaat aagggcagac gactggcatt
     6661 tctttatcat tatgttcgac tacattattg acttcttcgt atgcaaattt ttttccgcaa
     6721 ttagggcatt tcattttttc catgttatct caacttcctt tcatctttac tctcttagct
     6781 tactcttata ttgtaaaaaa gagaagcaat atccttcttc tatgcagata tccatacctg
     6841 acggtcctgt tctccatcta ttaatcggac tttataataa ggacgttcat ctttgactag
     6901 aagtgtccac tctttcactt ttgctttttt cccgtagtag ctgactgctt ccttcatcgc
     6961 tgcttgcggt tgtttgaact cttccgtttt ttctatatca aaaagatgtt ctgcggcctt
     7021 ttccgtgtca tttcgtacgg ttgttttccc agtggatgga ttgactgcta tgacttgtcc
     7081 ttcacaaaaa aactggtatt catatgttgc attcgtagga ttctccaaat ctttttcaac
     7141 tgcaaaatga atagcagata tgttatgttc ttttgtttcg ttttttacga ttgctgctgc
     7201 ttttttcaat gagatccgat attcctggtc actaatatca tccatgtgaa aagcggcatt
     7261 gagcagtccc gtcataccgg taatccctat tacgatgagg gcagcaacac caaacatcat
     7321 ttttttcatt cttcattctc ctgtttcttt tttttaaaga atatcaagaa atgaacgaat
     7381 gaaaaatcga atgaaacgaa aaaaaagtga caattttgtt atctttattc gtgcagttct
     7441 aatggcaatc cgtctggatc aaagaaaaac gtcatttttt ttcctgtaaa ggtatcttct
     7501 ctaatcggtt ctgattcgat ccctaaggac tgcaattctg caattgtttc ttcaatatcg
     7561 tctactttga aagcaagatg acgcaagcca caagcttctg gatagtttgg tcgttgtggc
     7621 gcattagcaa ttccaaaaat ctccaattca gcacttccta gtttcagatc taatttatag
     7681 tcatctcgtt ctttgcggta attttctctg attacttcaa atcctaattt attaacataa
     7741 aaatcacgtg atttctgata atctgaaaca atgatggcta taggatggat cgttgtaagg
     7801 ttcatatgag cactcctatt cttcgtttaa gctcggatct atattaatct ccagttccat
     7861 tgtaaatgtg tcagattgaa aaaaatagct gtttttcctt tttataagaa aaaaggcaaa
     7921 aaaaacagaa agtaggtcct ccaagacact cttcctgttc ccaatataat aaatgaaagt
     7981 aagaaattat attttcaaga aaacaaacag aaaattatca taacacaaat tatttataat
     8041 tgaaagacta gttttttttc aagaaaagca tacaatagat tgtgttagta aatacagaac
     8101 acactacatt tagtgtctta aggagtcctc atacatgaag tcatattttc agtttttagc
     8161 tcatcagcta aaaggctggc cgcaacaaaa ttattatctt ttctttttca gtcttggctg
     8221 ccaggtcatg actcttgtca atagtcccat cacttggctg tccgttatta catttatcgg
     8281 taccacgctt ggtgtattgt gtattttatc catcaatgcg gcaaaatcag tcaacggtat
     8341 tttaggtatt ttgtctgcga tttgttttat tatcgttggg ttttcagcaa agaactattt
     8401 aagtatcggt gaacagattg cttatatgat cacattggat atccccgtat tgttaagtaa
     8461 agaatggaat cataatatgg cttcaaaaat ccgaaaatta actggaaaaa gctggtttat
     8521 cgcaatcatc agtactttcc ttgtctatct tgtttcagga ttcctgattg gtcgttatac
     8581 agatgatcct cggccgtggg tagatgccat tgctttttct attagcttga ctgctggtat
     8641 catcagtttc ttgcgttaca acaaccaata ttactggtgg ttagcttcag ggcttgctca
     8701 aatggtcctt tggtatattt catttacgca tggatctgca agtttggcga tgttcgtgaa
     8761 cagctctgtt tacttgctga atgatgtcct tgcatttacc gtttcacctt ggtataacca
     8821 aaaagaacgt gaacgtatgc aaaaaatgga agaagaatac tacaacgcac aacaacacgc
     8881 ataaaaaaac tgagcgtggg acaaaagtaa agaacacttt tgtctcacgc tctaaacacg
     8941 tataaacggc ggaatcagaa gcaactcctt cggaaataag ctgaaattca caaaaatttg
     9001 aaaagcaatt ttcgtgaatt tcttcttatt tcttggagtt aaacgcttct gtcccagcct
     9061 ctatttttta tagtaattcc ttcagatact ttcttgcctc atcattgaaa cgcagtgatt
     9121 cttctaactt ctgataatat tccaacgctt gtttctgagc ttcagtaaaa cgataatgtt
     9181 ttcctttcca tttgtcgatt gtctcttgca catttccaga ttggatatct ttcactacta
     9241 gttgccattt ttcttttgag actgtacggt ctttcttatc agcagattgt atagcttctc
     9301 gttctttacg caatactttt aaaaaaccat ttctgactat ccgaggaatg ctgtcgaaga
     9361 acccttctgt aaaaccatag cgacaatatt gcttccaaat aaatagatag atttttggtt
     9421 ggatgaatcg ttggtttttc gcaaaatatt ccatctgagc agtgacttct ggtgttcgtt
     9481 gacgttttgt ctttctcaac acggaaataa atgtttcaaa ataagcatta ggatgtactg
     9541 gcacttcttc atatacgatg gattgccaat ctttacgtaa aaatgcgcga tagttgatca
     9601 agaattcttc tacatccttt actaattctt ctgcaaagtc gtcaaatagt tcttttttta
     9661 tctgtcgctt ttttgactgc ccattgatgt gttcacttat tttcttgatt tcattttcca
     9721 atgcttgata atccgctatg taaatcggca tttctacttg gcgaaattct gcgagccgct
     9781 gagctaattt tatatccggt aaatatggct gtttcatccg acgattttgg tacaagacaa
     9841 aacagtattt ttgccatagt ccttcaggaa aatcgatatt cttctgctta agccataata
     9901 attcgtctaa ggccagatca acttttgtca ttccctgatg gatcgaagct gccactgtcg
     9961 gagagacatg caggtgatcg gcaaagtggt tgatgcctag ttttaggatc tttccggttt
    10021 ttggcgattt aacgatgtat tgcactttta attctctacc acactcacaa tacaattttt
    10081 gctggcttcg ttttgttcga aaaaacggat tgatcttgaa gtcagaaaac acccaatctg
    10141 ttgcagctaa ataatgttct tccataaaac gagagctgat tagatatcgg cggtgcttat
    10201 cgactagttc ccgctgtttt ttcgttaatt ttgcatagat ctcccaacgt tgactgcttg
    10261 tgtattgttt agaaattttg atgggatagc ttttgctgat ttcttttgga aaccattctt
    10321 caaagacgga ttgccattct tccacggctg tttctttcct ttctaattac aaaaagctgg
    10381 ctactaacat aacgttagca accagccagt gtttcttgct aaattagtga gttgttccgg
    10441 catttccgga aatatcttct ttattttcta ctgctgcttc aactgcataa gtcaaagaag
    10501 aagcaatgtc ttctaaagac atagaaggcg tacccactgg acgatcgatc acttgatcgg
    10561 gtgagaatgg cacgtggatg aacccacctc gaattgccgg gaattgtgtt tcgatcaaat
    10621 gaagcaaacg atacatgata tcattgcaga caaatgttcc tgctgtataa gagatgtacg
    10681 ctggtagacc atgatcttgt acattttttg tcattgcttt gattggcaat gaggtaaagt
    10741 aagcggctgg accgttttct tctaaagccg tatcgaatgg ctgatttcct tcattatcag
    10801 ggattcttgc ttctgctaga ttgattgcga cacgttcgaa cgagactgct gaccgcccac
    10861 ctgcttgtcc gacacagatc acgacatctg gttggtgctg ttggatggct tctttgacta
    10921 cttgactgct tttttcgaat acggtcggta cttccacttt gatgatttca gcaccgctga
    10981 tttcatctgg catttttttt actgcttcat aggctggatt cactttgtcc ccaccaaatg
    11041 gatcaaaacc tgttactagt actttcatct gttgttcctc cgtccgattg atttaacata
    11101 tggttatctt ttatcatacc attttccttg aacagcgtaa ataaaggaaa caatagttta
    11161 aataacataa cagatcaaac ttaatatatt cttataaaaa aatagtgttc cactacaatg
    11221 ttagaagagg ttgcgacaat tattctgtca caacccctct cttctattat ttaaatagaa
    11281 tcatataagc aatttggaaa actagcataa ataacgcaat aaacaactgg tttttgatca
    11341 ctccgtattt gtctttcatc tccagcatcg ctacaggcac gatattgaag ttagcagcca
    11401 tcggtgtcag cagggtccca caaaaaccgc aagtcaatgc aaccattcct attaacgctg
    11461 gattcgctcc atgactaaat acgaatggtg caccgatccc aaccgtcatc acggtgatgg
    11521 ccgcaaatgc attccccatg attaccgtaa atatcaccat gccaagagcg taaatgatga
    11581 tgccgatgtt cacatttcct tctggaacga tcgtacctac tgcattggaa atcactgtcc
    11641 caactccagc gcttgtaaat accgcaccta gagaagctaa aagcattggc aacatgctta
    11701 acggccctac tgtgcccagc atatctgctg catcatctaa aaatgtactt ggtcgattat
    11761 ctcgagaaaa gaacatcaag attagcatgg ctgcaaaaac accgacgccc acgccgacta
    11821 aagcacctaa attggtaaat agagcaaaca agatcgcaaa gacaccgata ctcaaagctg
    11881 gaataaaaat tttcattcct agtttgtctg acattttttc catatattct tttgatggaa
    11941 gccggctttc tcctttgctg actttcttag caattgctgg tatggtcatc gcaaagatca
    12001 agccaccgtt cacgattgcc ggaagccatc ttcctcctgc aataactacc cctaagaccg
    12061 tccagaaaaa ggctgaccca taacgatgag gattggtttt atctgttaaa tttcttactc
    12121 cagtgtaaat caatacgagt cccatcacga tataaaggat ctccagtaat ttatcgccta
    12181 ataaaatatc aggatttgta aaaaagtcca tcttattgcc cctttcctgg ataatatttc
    12241 ttcgctaatt tcttttcttt aagatagaag tagacacttg ccacaactaa agccgtgata
    12301 gccactggaa cttccactaa agcaagctgt cccaatgtta cgtcatatcc tagatctttc
    12361 agtgttgatt ggactagcaa cgcccctgct cctccgatga atagtacttg gccaaagaac
    12421 cagcagacat tttccatagc tgaagccata ccttttagtt cttcttcatg cttaggatgg
    12481 acttctaacc cttttgattc gatggtcccc aatgccatcg gtaaaatgat tggacgaaca
    12541 aatccagcaa ctccaccaaa actcacgtta aaagccgcaa agatcattcg aaagatccca
    12601 taaatatcta caatcatacc agcagatact ttcttgaatc gcttgatcaa ggaagccgct
    12661 gattctttca gaccatttct ttcaagcgtt cctgttgcca acataatgat gataaaaatc
    12721 gccatcccgc gattgtttac aaaacttgta cctaaaagtt ctaacattcc ttgaatgcca
    12781 agaccgccag tcaaagctgt aacaactaaa gcaaccatta cgatcaatat cgagtctaat
    12841 ttaaatgcaa acccgagaat aacaatcaag acgcctaaaa ggctaagata attttccata
    12901 tattttcctc cataaaatga caatattttt ttaattataa agcaaaacac ataaaaaata
    12961 tagttgtttt ctcacgaaat agacactatt tttaaatatt tttcgttatt tttttattat
    13021 ataattcacc ttacttattg taagataaag ctttttttaa tttattgcct gctaatatat
    13081 aaaaataagg tctagttact gtttttttaa atgattaata aataacaact cctttctatg
    13141 aaattgcaaa gcattcatct tcttttctgc taacgatata tccgtcaatg cttgttacac
    13201 tttactaaaa agtatgagat tgccgatatc tggataggtg tgctatacta cagaaaaaga
    13261 tatacgatct gccaccgggt agtaaaaaag aggatgatca ttccattagg tctttgaagg
    13321 gatgattctc ctctttttgt gtatttttta tattgaagga ggaacgtatg tatggcatgg
    13381 atggaattaa tcatagcagg aattttggaa gtattctggt caacgatgat gaaatggagt
    13441 gatggttttt ctaaaatcaa ttattctttt tacacagtga ttggaatgat tgctagtttt
    13501 tactttttgt caaaggcaat caaaagtttg cctatgagtc tctcttatcc tatttggaca
    13561 gggattggcg cagttggttc ggtcattatt ggtgtggtct tgtttcatga taagctgaat
    13621 ctttccactt ggttctttgt cgggttgctg ctcatcagca tcattggcat caaaatcact
    13681 agcggtcact agatacttag acaagcagac aaggactgat cagaaaaagc ggggcttttt
    13741 atttgatatt cctctttcta tgaaaaaaca agaaaataag actaatgacc tagcgacttc
    13801 tctactcatc aggtgtatgc ttgtactatc tatagaaaag gagtgaatat ttttgaaaag
    13861 tctttcggtc caagaattat ccaagaactt tggtcacaaa caagccctca gcgatgtcag
    13921 ttttagctta tctcctggca aaatcgttgg attagtaggt cctaatggcg ctggaaaaac
    13981 aacgatcatg aaagctattt taggactgat ccattactca gaaggaacga tccgtattgg
    14041 tgaaaatgag atttctccct ctagtcataa aggattagaa caagtaggag cattgatcga
    14101 atatccgggt atctatccat tcttaacagg ttatgatcat ctgaagttat tctctgaaac
    14161 aaacgatgta tctgctatcg acactatcgt gaaacagctc aagatggaaa aatacattca
    14221 caaaaaagcg aaatcctact cgctgggaat gaaacaaaaa ttgggaattg ctcttgcctt
    14281 actgaaccat ccgtctatcg ttattttaga tgagccgatg aacggtttgg atccacaagc
    14341 aacaagagat gtgcgaaaaa tcattaccga cttggctact cagggcacct ctttcttgat
    14401 ctccagtcac ttactcagcg agcttgaaaa aatggtcgat gagctgatcc tgatcaatca
    14461 aggaaaaatc atcaaacaat gtacaatggc tgaattgaac caaacagcgc aagatttcat
    14521 cgttatctct acgacaaatg atcaagctgc tcgaacgatc ttgacagacg ctggatatcc
    14581 cttggaagat cctgataaaa tcaagcttct aaaaaaaaac gaagagttat taggaactgt
    14641 tatccagtta ttgtctgcag aaaatgtcca tgtgacagat gtccagcatc tgacgaatga
    14701 tttggaatct tccctattgg aaattctgga ttcagtaaag ggggaagaag aaaaatgacg
    14761 ttgcttataa aacaagaatt atttaaattg atcaagaaaa agtcaactgc tgttttatct
    14821 gttctattag ttgctttgct cataggaaca gcacttctag ccaaaaaata cacgacgatc
    14881 attgatccag ttgaaatgac tgctcagctt ttcagtgcca catcttggat tgtctttatc
    14941 atgattgctg ctgcaagtac gatcatttca atggaatcac aatatggtac attgaaaaat
    15001 ctcctttatc gaaagtattc tcgaggagaa atattagtaa gtaaatggat cacattagtt
    15061 atttattcta tttatttata ccttttagct attattgtca ccgtgctgat gaaactgatc
    15121 cttttccctt cgatttcctt tacggagaag atttctactg gtcagacact gatccaaagc
    15181 ttattcactt atactttggg tagttatatc ggtttatggc taattttaag tttagtgttg
    15241 atgattgctt gttttatcaa tagttccggt gcctccatct cagcagggat cgtcttttat
    15301 tttgcttcat caatcatatc tggtatcctc atcgcactca ttcaaaaatg ggagtggatc
    15361 aaatggaatc caatcagtat gctgaatctg caaaaccaaa ttggcaacga agagattatg
    15421 aagcaattga ctcatctttc tactaatcaa atgctgtttg gcaatttagc ttacattgct
    15481 ttgttcttag cattgggata cctcgtattc aagcgaaaaa atgtatgatg gttcattaac
    15541 aaagaagttg tgacagaagt ggccagtttc aagaaatttc ggcttatttt tagaagaagc
    15601 tacttcttga acaccattta ttcaaaaaaa gaagcgatga cacgaatgtt gtcaccgctt
    15661 cttttttgaa taaaccacag gcttcacagc ctctctttat acttctatac gtgaagcatc
    15721 ccagtggcaa ataaaaccat tgcgattact ccgaccatcg tcacaccgat aagtaaaatc
    15781 ttatctaagt ggatatgttg tacaggttcg ttttgttctt tacgtgcttt gaggtaaaaa
    15841 taaatacctg gtaagaaaag aacaagaaca agcatcactt cattaaaggc tacaactagc
    15901 atggcccata cttgaaaagc acttgctaaa aaccctacta aaaattggac ccactctttc
    15961 cgctgataag aaagcttcat ttggtacagc cctaagaaaa gccacgtgaa gaaaatcgct
    16021 gctgtaccaa gtgtataagc aaagttatat gcactgtcag tgaataataa tgaaagtaaa
    16081 aagacattcg tacataacgt cgtcaatacg atagcataag ttggtgcccc atttttgttg
    16141 agttttcccc atgctttagg caaaagtccg tcctcagcca tattccccat tgtttcaatc
    16201 ggcaagattg tccatgagag ccaaacccca actaaagaaa tgatcaaacc gccgttgatc
    16261 acgaccgccc cccatttacc aaccatcatt tcaaatacat atcccatagc aggttgcgct
    16321 ttgatcgttt gcagctgatt catcgtcatt gtgccatatg gaagcatgga aaccaagacg
    16381 taaataatga tcaaaccgaa tattcctaga atcgttgctt tcccaacatc cgattgtttt
    16441 tttgctcgtg aacctacgac agaagctcct tcaataccaa cgaataccca gagcatcacc
    16501 atgatgcaac tcaatacttg actagaaaac gggatcggct gcccattcgc attcaatatg
    16561 atatcgctag aaaagttatt aatgaattgt tggatcgaaa aggtgctgat agctactaca
    16621 ataaataaaa ataatggcac taatttacaa aatgtgacga tagcattgac aaagctagca
    16681 ttttcaactc cacgattcac tagccatgca tatccccata agaagatcgt actcatcacg
    16741 atcgatgcca cattttgacc accttcgaat acggggaaga aataacctaa tgcactcatc
    16801 aacaatgttg cgaacgccac gtttcctaac cagcatgtga gccagtaacc ccaaccgctg
    16861 ataaaaccag cgaaacggcc aaaaccttct tctgcgtaac taaagatccc gccttttaaa
    16921 tctggacgtt ttttattgat attattgatc gataaggaga gcatcaacat tgctgttcct
    16981 acgagtaccc agctgaagat agctgggcca ccagccgatc cggagatgtc cgtcataata
    17041 ccaaatacgc cacctccaat cgctgaacca atcactaagg cagcaagtgg aatcaaacca
    17101 attccttttt tctgttgctc tgtatccatg taagcatcct ccaaagtaaa accgctttta
    17161 tcaaaaagta taacaaacct tttttatttt tacttaatta ataaaaactt taatagaaat
    17221 ataattctac cgactttttt ctcattatac aagcaaagcg cctagtccct ttccaaatta
    17281 caccaccaaa aaaacttaaa aagatgctat actaaaggga aagaattggg ggatgatcca
    17341 tgaacatttt atatatatcg atttcaggaa atacgcgtgc ttttgccaaa catctagcag
    17401 aatatgctga aaaaatgcat gcagaagatc ctgtcaatcc tgaagttact ttaaaagaaa
    17461 tccatgaaaa tagtgatttt acgaaagaaa cagaaccatt ttttactttc gttcctactt
    17521 atttagacgg cgggaatggg ttagacaacg gtgacacaga aatcttgacc gaaacgatgc
    17581 gcgaatatct ggaatacgaa gacaatcata aactttgcct tggtgtagtc ggtagcggta
    17641 ataaaaactt caacaatcaa tattgtctga cagccaaaca atacgcacaa agattcggct
    17701 ttcctttctt ggcagattat gaattacggg gaacaccatc tgatgtgaca cgcgtttatc
    17761 aaattttggc agaaaaaaat aaataacaca aaagaagaag ctgaaaaata aatttcggct
    17821 tcttcttttt atgttctcta ttgtttctat tcttattaag ctttgacatc catcacaggt
    17881 gcttttgctt tcgctttacc tgttttttcc aagacaaagt tttttacacg atccatattg
    17941 gttaccaaga cgatcatgga ggtttctttg tctgctgctt tgattgcttc taagtcagct
    18001 tctgctacaa gcgtatcagg tgtcacttgc tgtccttctg ttactttcac atcgaatggc
    18061 gtaccgccta agtcaactgt gttgatgccc atgtgaagca agatttctaa gccatttggt
    18121 gtcgtaatgc cgatagcatg ttttgttggg aaaacagaac ttattactcc agtaaccggg
    18181 gaatagatat gtccgttttc tggttctaca aagtaacctt cacccatcat tttttgtgca
    18241 aaaactgggt cacttgcttt atctaaagaa acaactgttc cgtcagcaac tgatgataag
    18301 atagtcacac tttcagctga agcttgaggt actgcttttt caggtacttc atttactgtg
    18361 gctgtttcgg tcgctagttc ttctttttct aaacggtctt taggaatacc gaagaagaat
    18421 gtagcaacga agccaccagc gtaagcagcc aataaaccaa ggatatagcc taaccattta
    18481 ccatcagaga tcaatggaat caaagcggct cctgaaggtc caatagcgat tgctccgaca
    18541 tttccgatca ttccgatcac tgccccaccg atacctccac cgatacaagc agtaataaat
    18601 ggacgtccta gaggtaatgt tactccgtaa atcaatggtt cgccgattcc taagattccg
    18661 actggtaaag cccctttgat catttctgtt aattctttgt cttttttgca gcggatccat
    18721 aatgccaatg ctgcacccac ttgtcctgct ccggccatcg caaggattgg aagcaataag
    18781 gtacgaccag tttcatcaat catttgtaca tggattggcg tcaagatctg atgtagtccg
    18841 aacatgacca ttggcaagaa taatgtacct agaacgaagc cggcaaatgc tcctccaaca
    18901 ccaagtaccc agttgatccc accgaccatg ccgtcagaaa tgaaccctgc taaaggcatg
    18961 atgaagaaaa tttcgatcag accgatggca atcagggaaa gagttggcgt aacgatgata
    19021 tctaccgcat caggtactac tttatgcagt tttttctcaa cgattgacaa taaccaaact
    19081 gcgaagataa cacctaagat cccgccttgt ccagttgcaa gagcttcacc ggtaaacaag
    19141 tttttcagtg gtgcttctgg attcatccct gtcagcatca caacagcccc gataactcca
    19201 ccaagcgttg gcgttgcacc aaaaacttgt gcagcattga tacctgtata gataactaga
    19261 tagctaaaca tcccgttttt caagatgttc atcacatcga tatattgttg ccatgttcct
    19321 gcgtctagtg ttccagctgt gactaagttt gctaaaactg cggcaatccc tgccacgatc
    19381 ccagtgccga caaatgcagg gatcattggt acaaagatat tagagatgtc ttttaatacc
    19441 gatttgattt tagagggttt ttgtttcgat ttttgtgccg ctttcatttc ttgtgcccgt
    19501 tcgtttacac ggtcttttcc agatgcagaa gaacttgctg tagttgcact agcagaagga
    19561 agtttttctc ctaatttcac gcctgctaag tcaaccattt cctgcgctac tttgttcact
    19621 ttcccaggtc cgatgatgac ttggagctgt tcatcctcta ctacgccaag tactcctgga
    19681 atcgctttta attccgagac atttacaaga tcatcattga tgaccttcat acgtacacga
    19741 gtcatgcagt ggacaatgct ttcaacattg cccataccgc ctgctccttg ataaatttgt
    19801 tttgccagtt cttccaaagt caattcgttt tttgccatgg tcttttcccc ctcataaggt
    19861 tccttttaca aaaccgtttg cttcagctaa ttttgcttga gctgcctctt tatcactatt
    19921 tgtcaaaatc atgacgatag ccagtttgac attttgatct gcttcctcaa agtacttttc
    19981 agctgtttcg tagtcagctt ctgtcgcttg catgatgatt cgttttgagc gttcaattag
    20041 tttttcattt gttggtttga cgtcgaccat caagttattg aatactttac cgataccgat
    20101 catactgata gtagaaatca tattcaaaat cagtttttgt gctgttcctg acttcaatcg
    20161 agtagagcct gtcaaaaatt ctggtcctgc atcgacttcg attgggaaat ccgcatgctc
    20221 gctgatttct gcttgtttat tgcaagaaat cgttcctgta acagcaccaa tcgattttgc
    20281 gtaatctaat ccaccaatca catatggtgt acgaccgcta gctgcgatac caataactat
    20341 atcatttttt gttaaattca gcttgattag atcttcttgc cctaattcat aagaatcttc
    20401 ggctccttcg acggctacag tcattgcttc ttctccacca gcaattaggc ctatgaccat
    20461 ttccggttct acaccgaatg tcggtacaca ttcagcagca tctaatacac caagtctacc
    20521 actcgttcca gctcccatat aaatcaagcg accacctttt ttaaaggatt caatcgtgtt
    20581 ggcgatcact ggctcgatac ttggcagttc tttttcaaca gcagcggcca ctttctgatc
    20641 ttctttgttc atgagttgga ctgcttgaga aatactcatt tcatctaatc caaaagtttc
    20701 ttggtttctg cgttccgttg ttaggttctc taaattgatt ttgctcattt ttatcttcct
    20761 tcttttctta taaattgata gttcatacca gctttgattt ggttgatcag cggtagttct
    20821 tctttgatga ctttggctac tacattcact ttttcatccg ctggcaaatc atatttagtt
    20881 aattggatct cacccatata gcgcaaatac ttttcgttat ctaaagtaat agatcctttc
    20941 atacgcttag ctgtatttct tactgggatt ggcggaatct ctcgaaaacg cgcatctgcg
    21001 cttcgtatga catctcttgc atcatcttgc cgattcgtat gttttcctaa tatatattcg
    21061 taaaaaactt catctacagg ttccgtatgg agcaagactg tctgttcttt ttggaaagaa
    21121 gcaaattgct cctgaacgtg ttctgacaga cctgcatctc cgatataaac aatatccgtg
    21181 ttacttgcta atagatcaag agcacctgcc agaggatgga tccctcgatg ttcttccaaa
    21241 gttggtaatc cttgatacag tggacctctc aactctgtat ctcctggtac aaagccttgt
    21301 acaaggaatc cttgagtttt gagccattga tttttttcct gataccattc tttgtccaac
    21361 cctgtttcaa gacgtggata ataattatgc caagcttcaa tctgtgagaa atcagccgct
    21421 gcttctttca actcatcgat atcattaggc gtgatcgtgc tggcattcaa actgattttc
    21481 aattgatgtg accagtcggc gatctgctga ttagaaatat gataatccat tcttaaccct
    21541 gttacaccaa ttgctttcaa gggtgttaga tcttctaatg aaaagccagc tcgtttcaat
    21601 gcctctcctg aaatatcgac catcaactct agtttattct ttttcgcttc gcttcctagt
    21661 tcgatcaatc gattttgata agccgaaaca tcatcttccg gtatatgcat ggaagtgaaa
    21721 ataccgacaa agccattgga tgccatttgg cggatatact gtttcttctc ttctgacaaa
    21781 gaatcattca taaatattga aaagccaaac atattatccc tctttttctg tatccgtctt
    21841 catttctatg actagagtat atatctaaag aaacaaaatt tcaatacttt tgttaaaata
    21901 attaaatttt atttctaaaa attggattta taatgccaaa agaattgaca cagccacaaa
    21961 aaaggcactg ctaagcaaaa gcagtgcctt gaaacatttt atttaaataa cacaaaagtc
    22021 taattactca taagaaatta attctgtatt gtctaccatg aatgtaaaga acccttcatt
    22081 aggcattgga tgaaaataag cattgatcac tcttatcaag gcattatgac agactaacaa
    22141 gtagacattt tcttcgtctt ccatgcattc tttcaataaa ggaacgatcc gtgcgtacac
    22201 atctaagacc gactctccat ttgggaaacg tacagcaaac gctaaacgcg ctttttgaaa
    22261 gttttcatct ttgcttggca gtccgtcata atccccaaaa tccatttcta ccaatcgctc
    22321 atcagcggtc atcggcaaat tgagacgatc aactactgct tgtgctgtat ctcttgctcg
    22381 ttgaagaggg gaatggatga ttttcgtgat tggaacaggc aaatctacta ccttttctgc
    22441 tagtgcctgt gcctgcatct gtcctttttc agtcagcggc acatctgctc gcccgcaaac
    22501 taatccttgt gcgttccaag ttgtttctcc gtgtctcgtt acataaagca tgtgattctc
    22561 tccttcgttg acaaagttct ccttaaccat accttgttgt ctttgagatt acaaggttta
    22621 gattttctca agagaaataa tctcagtaga tactttatct agaaaggttt gatcatgttc
    22681 cactagtaac atagcaggtt tcacgttcaa aatcagttgt tctaattgtt cttgattgaa
    22741 gacatccaaa taattcaatg gttcatccca tatatagagc tcagctggtt gggataaaga
    22801 tttagccaat tccacttttt tccgttgtcc catactcatc tgttcgattt tattatgaaa
    22861 aacatctctt tccatcccaa gctttcggag attattcaaa aatgcttgat agtctactcg
    22921 gtttttcttc gcgaattccg ccaatgtccc tcgattgtct tcataatttt ggcgtacgta
    22981 actaatggtc aaatgtttgg gctgggattt ttctcctaca accttgccgt tgaatgtccc
    23041 cagaagataa tggataatgg atgactttcc cgcgccgtta ggacctgaaa ttgccatccg
    23101 ctgatgaggt tcgatcgtaa aatgaactgg ctcgaataac agattttcat accctaattg
    23161 aagattttct acgcttaaca gccgtttatg atgagacact tggctattca tcgtcaaagc
    23221 atcgatatac tcgatatctt ttaataattt ttctttctca ctgatctggg tttccatccg
    23281 attcacgatt gctttagatc gtttcatcgt ccgtgcagca tcagcgccta ctgctccttt
    23341 attcactcgc ctagattccg tatcgatgaa tccgactttt ttctttgttt tgtccccttc
    23401 tcgggaacga gaccattctg ctttttcagc tgctgttttc tttagcctgc tgacttcttt
    23461 tttcaatttt tcatttttag ctatttcaaa ttcatcacga agttttttct gttcttcata
    23521 gatagagaaa ttcccttgat aaagttccag ttggctcttt tcgattgcta aaacatggtc
    23581 cactacttca tcgataaatc cccgatcatg actcaccacg atgaatcctt gttttttctt
    23641 tttcaagtaa gccgctactt gttttctgcc agagatatcc aaatgattcg ttggttcatc
    23701 gattaacggg aaatgagtgt catccacaaa taagagtgcc agtaggactt ttgtcttctc
    23761 tcctcctgat agtgtgctga attctctcca taagatttca ggatctgttt gcatcagttg
    23821 gagctctctt tcaatttccc atatctcaaa atccataatc tcatttaaca cataataggt
    23881 caaacgctct ttatcttttg tttgctgcgg gaaatagaca aacgcttgct gatggatcac
    23941 ttgcccttga taagggagtt tgttttgcag gatattcagt aaagttgtct ttcctcgacc
    24001 gtttcgtccg ataagtccta gtttccattg tgtgtcaaag tttagattgg cttgttcaaa
    24061 taataatgtg ccttggctgt catagccgaa tgtcagattt tttatttcga ttttagacat
    24121 tattccatca cctcgtaaaa aaagaccagt cctattttca gaactggcta aagaggatgc
    24181 aagggttggg gcacacaaaa agacccatac atcgaaagaa ccaagccaat cccgaaaatc
    24241 tgcacactgc tcccttgtct ccacaaggta cagtctgaat gcagctttca tcaatcgact
    24301 taatttttca atgcacggat ccctcacttt gtcctatatt tgaaattacg atagcataaa
    24361 gaaattgttt ttgtcaacta cttcgggaga atgttgaaaa atctgctgag tgtctgtcct
    24421 aagatttctg ttttttctaa tgtttgcctg tgttccattg cttcctctaa atcgattggt
    24481 cctaattgaa tgcttaagac aatggggaga aaatcaaaga gtccttccag ctctcggtct
    24541 ctacttccgc aaatagcgat tattggtttt cgatattttt ttgcaagcac tgctacgcca
    24601 tatggaactt ttccgtggat tgtttgctga tcaatccgcc cttctcctgt aatgatgaca
    24661 tccgcttgtt cgatctgttt ttctaatcca gtgatttggc tgaccaactc aaaaccagag
    24721 accatcttcg ctcctaacaa aaaaagggct ccgccaatac cgcctgctgc tccgcttcct
    24781 gggatgtcat ctaaatccac ctcccaaaca tcttttatct gctgagcaat cttttcagct
    24841 tgtttatcca agaaagctaa cgtcgtatcc gttcctcctt tttgtcttcc aaattgccaa
    24901 gcagctcctt gttgacctgt atatgggttg gtcacgtctg cagcgatgat cacttttact
    24961 ttagaaacaa gaacgttcct tcgatccaaa tgaagttttc tagtagataa caaaggattt
    25021 ccttctgtgt acccttctaa agaagcaccc aaagattgga gtaatcctag accaccatcg
    25081 ctacatccgc ttcctcctaa agtgacaatg atttgttcca cttcttgaag cagtgtatct
    25141 ttgataagct ctcccaaggc ataactagaa gcaagtcgga tggtttgatc cgttggttta
    25201 tcaagtaaat ctaatcctaa tacttgtgca gattcaatga ctgcggtatt ttttccttgc
    25261 cagttcgtca ataaataact gatttttcgc tgacgaaata agagatccat cccatcaatc
    25321 gtacgccagg ttcccccgag cgcgtaatag attgcttctg cagtattttc tccgccatct
    25381 gcaatcggca cattgatttt tcttccttta cggtcttccc aaccatttag aactgcctga
    25441 tttaattcaa atgaagaagc acttccttta aatgaatcga ttgccgccaa aatatccatc
    25501 tatatccctc actttcttct taagtaagac actttttttc acttttttca aacaaaacaa
    25561 tgctattttc ttctgttttt ctatatacta aaagggaact cattgaataa aggagagaaa
    25621 aaacatgaaa atcgcaatag ctggagccgg agcaatgggc agccgaatcg gattaatgtt
    25681 gcatcaaagc ggaaatgagg tcgtcttgat cgatcgctgg cctgctcaca ttgaagccat
    25741 tcgtacaaat ggattgattg ctgactttaa tggcaaagaa gtagttgcaa aactgccaat
    25801 ctattcacca gaagaaatca tcgaaagcaa caaacacgtg gatttgatcg tagccttgac
    25861 aaaagcgaat caattagatg atatgtttcg ttcgatccaa tccattatta ctgagaatac
    25921 ttatgttttg tgtttactga atggattagg tcatgaagat gtactggaaa aatatgtacc
    25981 aaagaaaaat atcttatttg ggatcacgat gtggacagct ggtctagctg gtcccggaaa
    26041 agtcacatta ttaggtgatg gagaaatcga actagagaac ttggaaccag aaggcgaagc
    26101 atttacaaaa aaagttgtag aagtctttca agaagcaaaa ttgaatccaa tctatagtca
    26161 taatgttcgt tattccattt ggcgaaaagc ttgtgtaaac ggaacattaa atggattatg
    26221 cacgatattg gattgtaata ttgctgaatt aggtgcgcaa aaagcggcag agggcatggt
    26281 tcgaacgatt gtatctgagt ttgcttctat cgcagcaaaa gaaggaatca cattagatca
    26341 agaagaagtg taccaacata ttgcttctac ttatgatcct gacaacatcg ggttgcatta
    26401 cccatctatg taccaagacc tgatcaaaaa ccatcgtttg actgaaatcg actacatcaa
    26461 tggtgccgtt tggcgaaaag gacaaaaata tgatattgct acaccatatt gtgccttttt
    26521 gacccaatta gtccatgcaa aagaaggaat ccttggcgca gaataaaagg ctctctgatg
    26581 agaatcagct ggatcagcag ctggttctca tttttttacc cccatgaaag ttcaaaaaga
    26641 aaaacacaat tctcatgttg ccataagaat tgcgcttgtt tattttcatc tatgattggt
    26701 tggcactttt atttattttt aacagaggaa agatcgtatt catattgttc ataaatagcg
    26761 gagtcagtag tgaaaaaacg ttttgcccaa ttttctgcat caaaaaattc caacagttct
    26821 gatgggattg gttgaaatgg cgtatgcaaa aattccggta attcatctaa atcacgttct
    26881 tgaagcaaaa aaatattttc agaatgataa aactgataat gagctatggc ttgattatct
    26941 gtcactagtt tcttttgaaa atacatagat tcaaacgttc gcaaagtcac tccttgctgc
    27001 ccttcccgca acagttctag aatccccctc gattccccgg tttttttcag atattcttca
    27061 taagtaaggt atccaggttg ttcatttcct cgaagcggaa gaataaagta attggtcgta
    27121 acacctcttt ttgtaaattc ctgcttatat ttttctgccc gttcttttct gccgttattt
    27181 gttgcaccaa aaaatagatc cgtctttggt tcgctagaag aaaaatccat atgtttcgaa
    27241 taaaaggaac tattatgttt tagtccaagt tctttcgctt ctaaaaaatc aaaatgataa
    27301 atctcatcga ttgccggttc atcttgtgcc tgcttcagca tcgcctcgtg ttctgcttta
    27361 aagtggttcc aaaaaaataa cacgattttt gcattggtaa atggacgcac gcgtcgagcc
    27421 atctccatat caaatccata atctaaaaaa acgatcagct gataatttgc caactgttgt
    27481 cgagctgatt taatgaattc cgactgtagt aaatcttttc cggaccaaac atctaaagag
    27541 cggatggaag gaaaaaaata aagtgattta tttttcgcaa ttagcaatac gtcgtttttg
    27601 ttcatctgtt ccgcctcact ttctctgtca ctttttccta tgatagcctg tttaaatagt
    27661 aaaaagccag taaaacgctt cctattattt ttataaaaat ttaacatttc tatgaaaata
    27721 cgatgaaata aatcattctt ccattaaaat atgcccaaaa gttttctgaa tagttgtaat
    27781 tatc
//
LOCUS       DAZKKW010000030        27699 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.030, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000030 DAZKKW010000000
VERSION     DAZKKW010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27699
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.030"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(14..289)
                     /locus_tag="ACG8PO_001267"
     CDS             complement(14..289)
                     /locus_tag="ACG8PO_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0206607.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFRKQMKKLIEKEFPRSHKLYVNQQF"
     gene            583..1398
                     /gene="thiD"
                     /locus_tag="ACG8PO_001268"
     CDS             583..1398
                     /gene="thiD"
                     /locus_tag="ACG8PO_001268"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFQ0206608.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFKLIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKDAQQETVNDYVLLESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
     gene            complement(1603..3054)
                     /gene="cls"
                     /locus_tag="ACG8PO_001269"
     CDS             complement(1603..3054)
                     /gene="cls"
                     /locus_tag="ACG8PO_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFQ0206609.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQKALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            3396..6062
                     /locus_tag="ACG8PO_001270"
     CDS             3396..6062
                     /locus_tag="ACG8PO_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFQ0206610.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNE
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            6426..7178
                     /locus_tag="ACG8PO_001271"
     CDS             6426..7178
                     /locus_tag="ACG8PO_001271"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFQ0206611.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(7200..8354)
                     /locus_tag="ACG8PO_001272"
     CDS             complement(7200..8354)
                     /locus_tag="ACG8PO_001272"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004421 - hydroxymethylglutaryl-CoA
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HFQ0206612.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQELLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            8539..10980
                     /locus_tag="ACG8PO_001273"
     CDS             8539..10980
                     /locus_tag="ACG8PO_001273"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004420 - hydroxymethylglutaryl-CoA
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0015936 - coenzyme A metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HFQ0206613.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKPYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     KVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
     gene            complement(11310..12014)
                     /locus_tag="ACG8PO_001274"
     CDS             complement(11310..12014)
                     /locus_tag="ACG8PO_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0206614.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
     gene            complement(12201..12446)
                     /locus_tag="ACG8PO_001275"
     CDS             complement(12201..12446)
                     /locus_tag="ACG8PO_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0206615.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(12463..13314)
                     /locus_tag="ACG8PO_001276"
     CDS             complement(12463..13314)
                     /locus_tag="ACG8PO_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFQ0206616.1"
                     /translation="MNSLTSTYRLLNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQPTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            13479..14483
                     /locus_tag="ACG8PO_001277"
     CDS             13479..14483
                     /locus_tag="ACG8PO_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HFQ0206617.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
     gene            complement(14724..15080)
                     /locus_tag="ACG8PO_001278"
     CDS             complement(14724..15080)
                     /locus_tag="ACG8PO_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFQ0206618.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
     gene            15448..16635
                     /gene="tuf"
                     /locus_tag="ACG8PO_001279"
     CDS             15448..16635
                     /gene="tuf"
                     /locus_tag="ACG8PO_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFQ0206619.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            complement(16823..19414)
                     /gene="mprF"
                     /locus_tag="ACG8PO_001280"
     CDS             complement(16823..19414)
                     /gene="mprF"
                     /locus_tag="ACG8PO_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HFQ0206620.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLVSVLPMIGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDP
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(19440..20201)
                     /locus_tag="ACG8PO_001281"
     CDS             complement(19440..20201)
                     /locus_tag="ACG8PO_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFQ0206621.1"
                     /translation="MTDIIVILYTVGDYVKKEKKYAKMPDGSEIYYEKSGQGFPLFLL
                     HGNDGSGRFFSEQVPVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEK
                     IDQADFLGFSDGANLALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVW
                     LLSLFRPSLRKNLLVIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKT
                     IPKASFVLVKEQGHELARKDPERFNREVLQFLSET"
     gene            20368..21000
                     /locus_tag="ACG8PO_001282"
     CDS             20368..21000
                     /locus_tag="ACG8PO_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HFQ0206622.1"
                     /translation="MKRKRLSIIRILFYMIILTVIGAVLFLGGRTYRSVKRVESYQIE
                     ITTAAKNYHMENYENLIKAIILTESKGKGTDLMRSSESLHGEAEIIDNPADSIDQGVK
                     YLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHGGENTVELAEAYSKDVLSPLLG
                     NEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKMNEKIIETYEKVFK"
     gene            21059..21919
                     /locus_tag="ACG8PO_001283"
     CDS             21059..21919
                     /locus_tag="ACG8PO_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HFQ0206623.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            22104..24419
                     /locus_tag="ACG8PO_001284"
     CDS             22104..24419
                     /locus_tag="ACG8PO_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFQ0206624.1"
                     /translation="MNGLTQQEVVQKMASGQQNDYQEDASKSTKQIFSDNILTLFNFL
                     NFGIGICLLFVGAYSNMAYLAIIVVNIVIGIYQEIHARNLVRKLSIVSQSEVLVIRDG
                     KEQSISTKELVLGDLVKIGAGEQIPADLQVISGRAEANEALLTGESDLIVKETGAELL
                     SGSFLTSGSVMAEVIRVGADNYAVRLTSEAKTHKAIRSELVDSIRKVSKFTSFVIIPL
                     GIILFLEALFIRDASIKISVIASAAALLGMLPKGLVLLISIALTTGVTKLAKKRILVQ
                     DMYSIETLAHVDTLCLDKTGTITEGKMSIQRVEALHPLYEKAIPEIMGTYLAESSDNN
                     ITMRAMRDYFELSNRYDVTNKVAFSSDRKWGAMEFPELGVVYVGAPERLVEEDRLPDS
                     LKTAQANGYRVLMLGIAPNQQLNEEKPAFVEPVALFEIDDPIRKNAQETLAYLHEEGV
                     DLKVISGDNPFTVSNIARRAGLPNYDAYVDLSQITEEMEVREAAHRYTVFGRVSPQQK
                     KLLVNELKESGRTVAMTGDGVNDVLALREADCSIAMAEGDGATRQIANLVLLDSDFTT
                     LPEVLFEGRRVVNNVTKVSGIFFIKTIYSFILSIICAVTAIGFPFIPIQITLIDLAIE
                     GYPSFFLSFEQDKRKITYRYLPTALINALPNALLVVVNIIAVYLLGKTFAWGNAETTT
                     LMYYLLIGISCMAVVKACYPFNPLRIFLAVTTIVGIYVAAMLFHGILEVNLGLGESVG
                     YFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            24601..26442
                     /gene="dnaG"
                     /locus_tag="ACG8PO_001285"
     CDS             24601..26442
                     /gene="dnaG"
                     /locus_tag="ACG8PO_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFQ0206625.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            26466..27575
                     /gene="rpoD"
                     /locus_tag="ACG8PO_001286"
     CDS             26466..27575
                     /gene="rpoD"
                     /locus_tag="ACG8PO_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFQ0206626.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
BASE COUNT         8963 a         4896 c         5776 g         8064 t
ORIGIN      
        1 gaacagttag aagttaaaat tgctgattca catagagttt atgacttctt ggaaattctt
       61 tttcaatcag ttttttcatc tgtttacgaa attctttttt ttcagtaaac ataaaggttt
      121 gggtatcgta accttcttca tgtaggaaat agattattcc tttttctgtg ttttgcgtat
      181 aagcttttat actcctgtct tttttgaaag aacagatcgt atagccatct atttggtctg
      241 gtagtcgttt gattttcttc aaaatgactt ttttatcctc aagtttcatt tttttcacct
      301 gactttattt ttttcatatg gaaatcatag cgcctttggt tatatgaaaa caagaaggat
      361 actgaaatat atataaatca aatagtggtt atttattatt agaatagcga atacaaacaa
      421 atgacaacta ttttctgatt tattacaaat gttttttcaa ataaatttga ttaccaagaa
      481 aaggtagtgg tatatttact gagggatact ttgaaaaaca tatgatagaa acagtaacag
      541 cattatgaaa aatcttttta taaaaatgga ggaatcgatt tgatggaaga tcagtatgta
      601 caagcaatga cgatcgcagg atcagatagc gatggaagcg cagggatgca agcagatatg
      661 catacattct ttacaagaaa tgtttacggt gtttctgtca tgaccgcatg tgttgcaggt
      721 aattcttacg gaatcggagc aagtgtgaca ttgccaacag attttatcga caaagaattt
      781 aaactgattg cgaaggactt tcaaatacgt gcagctaaaa caggtatgct agcagattca
      841 aagttgattg agacagttgt aaaaaattac caaaaatatg attttggacc attagtcgtg
      901 gatcccgtaa tcgtaacgaa acatggaaat cttttactag aagaatctgc tttgcaaagc
      961 ttgaaagaaa agcttgtccc tttggccgaa gtactgacac ctaattttta tgaagcagaa
     1021 aagctgactg gacggacatt agcagctgaa gaagattttg tgcaagcagc aaaagaactg
     1081 caagatatgg gtgcaaaaaa cgttatgatc aaaggacatc atgctaaaga tgcgcagcaa
     1141 gaaacagtca atgactacgt attgctcgaa tcaggaaaat cattttggtt aaatgcacca
     1201 ttttatccta ccgaccggat caacggaact ggcgattcgc tatctgcatg tattgcagct
     1261 gagttagcga aaggaaaaac agttgaacaa gcgatccgat tggcaaaaga ctatgtaaac
     1321 cgtgcaattc gtgaagaaat aaatgtaggg cacaaatatg gacccatcaa tcattgggca
     1381 gctggtgaat tagagtaaaa gaaagaggtc gtgagcctgc cgtccagctc cgagtcacaa
     1441 ggaacaagtt caaaaaacag cttctcatgt ttgttgaata ttgtgacttg tgaccgagga
     1501 gttggcagga gaacaccgtt tgttcggaag aagggactgt gacaagattt ttgtcacagt
     1561 ccctttaatc ttagtatatt tttctctact tgaataaaaa aattatagga ttggagaaaa
     1621 taatcgagaa atttgttgct tgaataatag ccagttagac atttccttga ctgtttcagt
     1681 tgtcatggat gaacaatgtg tctcatcttc tttgaactga gtagcaagtt cttctaaaaa
     1741 ctcaggatcg taaatgacag cacttgtttc aaaattcaat cggtagctac gaatatcttg
     1801 atttgctgaa cctaccatgc agatttcatc atccattatc aatgtttttg catggaggaa
     1861 gccgttctca tagacaagga tttgtacatt ttccttgatc agctgccgag cataatattg
     1921 tgttgctcga taaataaaag gatgatccgg tttgttggga atcatgattt tcacatctac
     1981 accagaggca gttgcgattt ttaaagcagc aatgacactt tcatcaggaa caaggtaagg
     2041 tgtctgtatc caaacacgtt ttttagcaga agtaatcaat ttgataaatg aaagcttgat
     2101 ttgttccctt tcgttattcg gtccgcttga aacaagctgg atggatgtat cagcaagctt
     2161 agattcatct cgatctgctt ttttaaagaa ataattcaaa tgatagccga cctttttttc
     2221 ttcaggtacc gagacgttcc agtctgttaa gaagcgaagc tggagcaaag aagaagcggc
     2281 accgaaaatc cgtatatgcg tatcgcgcca atagccgaat ttttttgttg tatttacata
     2341 ttgattggca atattgaaac caccggtata actaatcttt ccatcgatca caacgatttt
     2401 tcggtgatca tggtaattca atcggaaacg aagtaatgcc ttttgtgagg taacaaatgt
     2461 atggacatgt ccaccatttt tgattagacg attgaaatct tttgcttttg taccatgaga
     2521 gccaaatgca tcatataata ttcgaacttc cacgccttct gcggcttttt cttctaaaac
     2581 atgtaagact tgctggccga tattatctgt cacaaatgcg taatattcga tatggatcga
     2641 atgtttggct ttttttagat cttgaagcag tgaatccaat ttctcttgtc cgtctgtgag
     2701 aagagtgaca gaattcattc ttgtcagcgg catacgattt aatgaagaga agaagtcgac
     2761 aaattgctgt ttgtctttat cgcccatgtc aggatcgtaa tgttcaatgg tatctcctct
     2821 aaaagactga aagttttcta attctttcaa gtcactttgt cggagataga attttttctt
     2881 atccgttaat ccacgtccga aaaataaata taatacaaag cccaccccag gaagggcaaa
     2941 caatactaag agccatgccc aaatggctgc tacatctcgg ggtttgatca aaatagccac
     3001 caaagcaata agtgcattta atagataaag ggcggttata atactagata ccacaaaaat
     3061 gttcctcctt acatacaata acattagtat agcatgatag aagaaagaag cctaccaagg
     3121 tgaatatact tttcactcct ttcatgaaaa tgtaggtaaa tcgggctaaa gtagttgctt
     3181 tttgtaggtt aataaggtag tatattagta cagttgaata tctccttagg ggagtaaccg
     3241 gcaacgtttg ttgcgataat atcaacagta agttaaccaa atggttttct ggtattgtat
     3301 gaaatggtga gacctaagac aaatgcgtat ttgtcttagg tctctttttt tttcagacag
     3361 gactagggcc aagatcaaaa aatgggggat gaaatttgga aaacaaggaa caagcacaac
     3421 aagcggtcta taaagaaacg atcgactcgt tgatgcagca aacgcagagc tcaaccgatg
     3481 gattgagcac cacgcaagcg aaggaaaaat tagaaagaga tggcttgaac cagctaaaag
     3541 aaaagccgaa gaagccaact tggaaattgt tcttagaaac tttcaaagat gcaatggtta
     3601 tcgtattgct gattgtcgca ctgattcaga tggtcatggg tgactacatc gaattcttag
     3661 ttatttttgc agtattgatg ttgaattcta taatcagtgt tgtccaaaca aaaaaagcag
     3721 aaggttcttt agaagcgttg aaaaaccttt cagcgcccga tgctcgagtg attcgtgatg
     3781 gtaaggaaaa aacgattccg gcaaacgaac ttgtggtagg agatatcgtg ttgttagacg
     3841 caggcgatta tgtacctgct gatggacgtt tactagatgc gggatcatta aaagtagatg
     3901 aaggtatgct gacaggagag tcgacttctg cagaaaaaga agtaacggat atcacaagta
     3961 cggtaccaat cggagatcgt acaaatatgg tattcagtgg aacgatcgta acttacggac
     4021 gcggtaaatt cttagtgact tcaactggga ataatacgga gatcggacaa gttgctggcc
     4081 ttttggaatc aacaactgag caagtcactc cgttacaacg aggtctggat aaattcagta
     4141 aaaaactaag tctggctatt ttggcattgt ctttattgat tttaggtatc caactgtttc
     4201 gtatttatct tggagaaggt acaggagata tgactgcaga tatcgtcagc gcagtgatgt
     4261 ttgccgtagc cgtagccgta gctgctattc cagaagcatt acagtctatc gtaacaatcg
     4321 tgttatcttt gggaacgaat aaaatggcaa aacgtcatgc cattattcgg aagcttccag
     4381 cagttgaaac acttggttcg acaagtatca tctgtacaga taagactggt acattgacac
     4441 aaaacaaaat gacagttgtt gactactttt taggtaatgg cgatactggg gatttcacaa
     4501 atgacccttc aaaatggaca gctgacgaac gtcgcttgat cgaaatcagt gttttagcga
     4561 atgatgcatc gatcagtgaa gacggtacta aattgggcga ccctacggaa gtagcgttta
     4621 tcgactttag tgaaaaatta aaccagcctt atcaggaaat ccgaaacaac tacccaagac
     4681 aagcagaact tcctttcgat tctgatcgaa aattaatgtc tactggacac acgatcgata
     4741 acgaaaatta cttgtttgtc aaaggcggac cagatatcgt cttccaacgc agtaaaaaag
     4801 tattgctaaa tggtgaagtc gttccgatga cggatgaatt gatgaaaaaa ttccaagagc
     4861 aaaatgaagc tttttcaaca cgcgcattac gtgtactagc ttttgcttac cgtccacttg
     4921 ataattttga tttatcattt gaagatgaag acgatttgat ccttgttggt ttgcttgcta
     4981 tgatcgatcc tccacgtgct gaagtgactc aggcggtaac agaagcaaga agtgcaggaa
     5041 tcaaaacaat catgattact ggtgaccaca aaacaacagc tgttgctatc gcaaaacaaa
     5101 tcgggatttt tgaagaagga aacctggctt tgaccgggac ggaactagat gaattgacag
     5161 aagatcaact aaacgaagtt ttagaaaaag tcactgttta tgcacgtgtt tcacctgaga
     5221 ataaaattcg tatcgttcgt gcttggcaaa ataaaggtca tatctcagct atgacaggtg
     5281 atggtgtaaa tgacgcacca gcattgaaac aagcagacat cggaattgct atgggaacgg
     5341 gtacagatgt ggcaaaagat gcagcagcga tggtattgac ggatgataac tttgcttcta
     5401 ttgtcagcgc agtagaagta ggacgtaatg tgtttgataa tatcaagaaa gctatttctt
     5461 atctatttgc aggaaattta ggtgcgatca ttgcgatcat ctttgcctta gttgctgact
     5521 ggtccaatcc atttacagca ttacaactgt tgttcatcaa tttagtcaat gattcactgc
     5581 cagcaattgc attaggtaca gaaaaagctg aacctgctac gatgaaccgc ccgccaagag
     5641 atccaaattc aggaatcttt acggacaaaa cattgatttc tgtaacgtat cgtggtgtgc
     5701 tgattgggat tgctgttatc tttgctcaat ggcttggcat gcagcattca cctgaattcg
     5761 gtgtagccat gtcattttca acattgattt ggtgtcggac attgcaaaca ttcccagcac
     5821 gttcaaacac acaaacagct atccaagcag ggttcttctc taacagaagt gtcttactag
     5881 ctgtagttgt atgtagcgcg ctttactgct taacaatgat cccaggactg aaagaagtct
     5941 tctctatccc atcagcattt ggattgacag aagtctttac atgtatggga atcgcacttg
     6001 tgacaattgt tttgatggaa atcactaaat tgatcattgt acgagtacaa ggaaataaat
     6061 aaaacgaatc aacgaggttg tgagcctgcc gtttaattct gagtcacaag gtgcaatttc
     6121 aacaaacagc ttctcatgtt tgttgaatat tgtgacttgt gaccgaagag ttggcaggag
     6181 aacaccgttt aaggaagaag ttgtaaaatc agcattttga gctaatactg agggtcatct
     6241 gattggacac tgtttattcg gaaagaggga ctttgacacg actttggtca cagtcccttt
     6301 ttttatttat gatagacgga aagccaatca ttttattatt catcaaaaga gatagaatag
     6361 ataaaagagg agaataattt attccgaaaa gtgaaaacat ataacaaaaa attagaggaa
     6421 atcaaatgaa tgaaaaagaa tgggatgaat ttgctgaaga atattatgaa atccagcaag
     6481 aatcgcaagt aactattgct gaagatgtaa aaaattatct gaaaacggag aaagtcctgc
     6541 ctgctgaaac agcagcagac gttgctggag gatctggcag atttctttcc ttagcaaaag
     6601 aaattaatcg ctatgatttg atcgattttt cttcaggaat gctagaaaat gcaaaaaaag
     6661 aagcagaaaa aaatgagttg ccaaatgttt cctttatcaa acagtcgtta gaagaattta
     6721 tccaatcaaa tgagacatat gaaatggttt ttacagcagc aaatccagca ttggtacatc
     6781 ttattcaatt agaaaaattg atgaaaaagt caagcaaatg gtgcgtgata atcagggtag
     6841 tggaatcaat cgatgatgtt ttttatccta tcgaaaagca actaggaatg atagaagaag
     6901 atcctaatac atcgccagtt gtaatggatc aatttgaaac atacttaatt gatgaaggat
     6961 tcaaagtgaa gaaaaaagat tttacttatt ttacacagga atcaattact gaagaattga
     7021 tgaagctata ctatgacgcg tacgcaaaag atcctgttgt gaccaggtac attcgcaaac
     7081 tattcagtga aaaacgagaa atccttagta cgactaagct gacctataga ctgctccttt
     7141 ggtccgcaga taaaaaaaga acctttcacg actcgtgaag ggttcttttt atgattttat
     7201 tatattttgt agtaacgaat atcattttcg atctttgtaa tcgaaaaagg tacatctgaa
     7261 gtatattctg cacattctcc gtgttcagga agcgtctcac tgaaaatcgt ttcatattcg
     7321 tcaacagtga tccgtgttcg agaatctagc atctcttgat gagattgtgc gaagagatat
     7381 tcttgatagt tttcttccag ataacctgta aaaaattcac ttactgcacc agaaccgtag
     7441 ctgaatagac cgatgcgatc tcctggctgt agtgatttcg aattttccag tagagaagtt
     7501 agccccaggt ataatgaacc agtgtaaaga ttaccgattc gtcggctgta acggatgctt
     7561 tcttcatagc gagccataag aagttcctga ttttcttcgt ctgtttggtc taatacgctt
     7621 tgcaatgcct tttttcccat cttagtatac ggaatatgga aagcaatcgc ctgataatct
     7681 tcgagtcctc gacccgacaa ttctttatgt cgattccaaa ctttttggaa tgattcgata
     7741 tacgtagaat tagataaagg accatcaaca acaggaaatt cgctataatc tggacgccag
     7801 aaatcataga tatcttctgt cagaaatacg ctgtcgtctt caatcgataa aatacgcggg
     7861 ttttgagtga tcatcatcgc aacagcaccg acaccttgcg tcacttcacc accgcttgcc
     7921 aagccgtaac gagcaatatc acttgctatg actagtactt ttcgttctgg atgatttttg
     7981 acatattctt tcgccatgtg caaagctgcg gtgccaccgt agcaagcctc ttttacttca
     8041 aaagaacgag caaaagattg gattttcagt aaatggtgga taattaccgc actcgctttt
     8101 gaatgatcaa tcccggattc tgttccgacg atgaccatgt cgattagctc acggtctttt
     8161 tctgttacga ttttgctggc agcatttgct cctagtgtaa tgatgtcttc atttgcacga
     8221 ttgactgcca tttggtcttg accaatccct atatggtatt ttgcaggatc atccccacgg
     8281 ctttctgcca actctgtcat atctaaatat aaattaggaa taaaaaagga aagacgatca
     8341 atccctattt tcatttcaac acctctttac ttatgataaa aaacttactt tactaaggta
     8401 tcataaaaag attaaaagaa atataaaaaa gagaatattt aaggattgtt aaaatttaat
     8461 cttagaaaaa aacaactgga acaagagcta tttgttttta cagcaaagaa tggtatattt
     8521 ttgtaggagg tggcttactt gaaagaagtc gttatgatag atgctgcaag aacgccgata
     8581 ggaaagtatc gcggaagttt aagtccattt actgcagttg aattaggcac acttgtgact
     8641 aaaggattat tagataaaac aaaactgaaa aaagataaaa tcgatcaagt aatattcgga
     8701 aatgtcctgc aagctggtaa tggtcaaaat gttgccagac agattgcttt gaacagcggt
     8761 ctccctgtag atgtcccagc aatgacgatc aatgaagttt gcggatctgg tatgaaagca
     8821 gtgatcttag cacgccagct gatccaatta ggtgaagcag aacttgtgat agccggcgga
     8881 acagagagta tgtctcaagc acctatgctg aaaccgtatc agtcagaaac aaatgaatat
     8941 ggtgaaccaa tttccagtat ggtcaacgac ggattgactg acgcattttc aaatgcacat
     9001 atgggattaa ccgcagagaa ggttgcaaca caattttctg tgagcagaga agaacaggat
     9061 cgctatgcct tgtcgtccca gttgaaagca gcacatgctg tcgaagccgg tgtattttct
     9121 gaggagatca tcccagtcaa gatttctgat gaagacgtgt tatctgagga tgaagcagtt
     9181 cgtggaaata gtacattgga aaaactgggc acgttacgta cagtattctc agaagaagga
     9241 actgtaacag caggaaatgc ttcgccattg aatgacggtg cctctgtggt gatccttgca
     9301 tccaaagaat acgcagaaaa taataatctg ccttatttag caaccatcaa agaagtagcg
     9361 gaggtcggta ttgacccaag tatcatggga atcgctccaa tcaaagccat tcaaaaattg
     9421 acagatagat caggaatgaa tttatcaaca atcgatcttt ttgagatcaa tgaagcattt
     9481 gcagcttctt ctatcgtagt tagccaagaa ctgcagttag atgaagaaaa ggtcaatata
     9541 tatggaggag caatcgcatt aggccatcca attggtgcaa gcggtgcacg tatcttgact
     9601 acattagctt acggattgct tcgtgaacag aagagatacg gtattgcttc attatgtatt
     9661 ggtggaggtt taggcttggc agtcttgtta gaagcgaata tggagcaaac acataaggat
     9721 gtgcaaaaaa aaaagtttta tcagcttacg ccatctgaac ggcgcagtca attaatagaa
     9781 aaaaatgtat tgacacaaga aactgcgttg atttttcagg aacaaacact ttctgaagag
     9841 ctctccgatc acatgatcga aaaccaagtg agtgaagtgg aaatcccaat gggcatcgcg
     9901 cagaattttc aaatcaatgg taagaaaaaa tggattccga tggcaacaga ggaaccatct
     9961 gtcattgcag ctgcaagtaa tggtgcaaaa atctgtggaa atatctgcgc agaaacacct
    10021 cagcggctga tgcgagggca aatcgttcta tcaggaaaat cggagtatca agcagtgatc
    10081 aatgcagtta atcatcgaaa agaagagctg attctttgtg caaatgaaag ttatccttca
    10141 atcgtgaaac gcgggggcgg tgttcaagat atctctactc gggaattcat ggggagtttc
    10201 catgcatact tatctattga ttttttagtg gatgtaaaag atgcaatggg cgctaacatg
    10261 atcaactcga ttcttgagag tgtggcaaac aagttacggg agtggttccc tgaagaagag
    10321 attctattca gtattttaag taattttgct acagaatcat tggcttctgc ctgttgtgaa
    10381 atcccattcg aacgcttagg gagaaacaaa gaaatcggtg aacaaattgc caaaaaaatc
    10441 caacaggcag gggaatatgc gaagcttgac ccttaccgtg cagcaacgca caacaaaggg
    10501 atcatgaatg gtatagaagc tgtagtggca gctacaggaa atgatacacg tgcggttagc
    10561 gcttcgattc acgcctacgc ggcaagaaat ggattatacc aaggattaac agactggcag
    10621 atcaaagggg ataaactagt tggaaaacta acggtgcctt tggctgttgc cacagtaggc
    10681 ggagcttcca atatcctgcc aaaagcaaaa gcatctttag ctatgttaga tattgattca
    10741 gccaaggaac tagcacaagt aattgctgca gtagggttag ctcaaaacct ggctgctttg
    10801 cgtgcattag ttacagaagg aatccaaaaa ggccacatgg gacttcaagc tcgttcatta
    10861 gcgatctcaa ttggggctat tggtgaggaa atcgagcaag ttgcgaaaaa attacgtgaa
    10921 gcagaaaaga tgaaccagca gacagctata caaatattgg aaaaaatccg ggaaaaataa
    10981 aaaagcaata tctttagaag aataaagcaa gatttacagt tattttattt actgcaaatc
    11041 ttgctttttt ctctatcaga aaatatgttt attattagaa ttttttatag ttaaggttag
    11101 gcatcttaag aaatcaggca actccaagat atcttcaggt agaagagaag tcgcctaaca
    11161 ttgtaggatc ataggtgatt tgttggacag gtcaagctga tatttgagcc tgatgttaga
    11221 aggaatttct ttttacttgc tgtagaaatt attatatgac taaaaaggct gggattttaa
    11281 atcccagcct tttatgacaa atagaatttt tatcgatcag taatcgctgt cgctgcaaat
    11341 tgttcgatca aaggaatcaa agtcaatttg actttgtatt tccgttcgag tgctttttcg
    11401 ataacgaact cattttcagt agtcatagaa atagttgcta atttacgagg ttcatcagca
    11461 tttttttcat taataactac ttcatgttca ttttgttctg tgtaaaattt aggatactca
    11521 gcaacggctt ttgaaaaatc agcaaaactt aaagcggcat tcgtatcagt tgagcgtgta
    11581 ttttcagttg tgtccgcttt tggtttttcc gttttttctt ctggcacact gactgtttct
    11641 tctgtaactg gtgtcgcaac agcttgcgca gttggttcat cgtttttcaa tcgttgatca
    11701 ttccagtaag acacgatttc aataagaaga ttgtcacgga atcgttcgaa aatcccgttt
    11761 tctcctgaac gaattctctt taatacagta ttgaagttgt catcgattgg cacgttgatc
    11821 cgtgtatctt catttgtttt cccagctaat ccgatcgtta agatttgacg gtttttggga
    11881 tcaagcatga cttgtaacgg caaaccatag cgtcgcaagc tctcttcttc tagagaacgg
    11941 aataattgtc ctcttacacc atccaatgaa ggacggaatt gatttaataa tttttgtttc
    12001 tgttttttct tcatatgttc tctccttcgc tcataccctt tcgattataa gaaaaaaaca
    12061 gccaaatgaa aaggagtaat gcaaaaaaac ttgcgtatac gcaaattaca acagaaaaat
    12121 ttgcgtattt tcccgttttt tgaaagaaaa ataaaaaatg gaacggatta tgtatatata
    12181 cataatccgt tccattcttt ctattttttc ggtatcaagg caaaaacagg tagttcaggt
    12241 gaataaatga cttcatgttc gtgccagggt ttgaccatta gcgaatgatc gaaccgtttt
    12301 ttcagttctt cgatgatttg atcatgtgtc cagtttcttg caggaaaatg ttggattttc
    12361 tctgcatcga tcaaaaagac aaaatcggaa tgaaaagtct gggtaacttc ttccgagaga
    12421 gaattcaagc gttcgattgg atccataaaa tcacctttcg acttaaaatt gggtagtatc
    12481 tgggtctttt gctttgccga ctacaccatc taactgatcg atttgtttca tttcttcttc
    12541 tgttaattca aaatcaaata attcagtatt ttctttgatt ctgcttggtg tcactgactt
    12601 aggaagagga aggaattcgt gttgcagcga ccaacgcaaa gcaacttgtg caatcgtctt
    12661 gtcatgtgct tcagcgattt gtttcatttc aggcacatcg aagattttac cagtgcctaa
    12721 tggactatat gcttcgagga tcatatcatg tttttttgca tactcaacta cagttggctg
    12781 taattcgcct ggtgcaagga aaacttgatt gaccatcggc ataatttttg ctgtttttgc
    12841 tagtgtatct agatggtggg gtaagaaatt gctcacaccg attgctttga ttttacctgc
    12901 ttcatacagt tcttcaaatg ctcgccatgt atctgcattt gcttgttccc aattatcacg
    12961 aaaagcaaca gggtttggcc agtggatcaa aaacagatct aaataatctg tttggagctt
    13021 cttcaatgat tcttcaaaag aagacatcac aagttcatag ctgtggtttg cattccataa
    13081 ttttgttgtc aagaatatct cttctctagg aattccactt tccttaatcg cttggccgac
    13141 actttcttca ttcttgtatc cttgggcagt atcgatatgg cgataacctg cagccagcgc
    13201 ttctttaaca gaggaaacag cgacatcccc atcaggtgtt tgccatgtgc cgaatcctac
    13261 taccgggatt tcgtaaccat ttaatagacg ataagtgctt gttaatgaat tcatactttc
    13321 agctcctttt ttattcgttc aactataagt gtagcgcatt tttcgaaaaa aagaatccat
    13381 tttgtttcta ctcttgaaat ttttgtttaa aagaaaatga ttttgattct ggtatttttg
    13441 atttttttcc gtaaactaag acagaaggga atgagaaaat ggatatctat ttaaaaaaag
    13501 caattctgca tattattgac agagaaactg gttctccagt cttttcacaa aaagagctgg
    13561 atctgacaaa agaatatatt cgtgattttc ttcaaaagaa gatccaaaaa atctcttctg
    13621 ctcaaacaaa aactggacaa ctagcagaag aaagcatgat cgttgaatca cttaaaatcg
    13681 ctcaagaaaa ttttgtggaa gcaagtgaag caatcgttca gcggtggttc gatatctacc
    13741 aagaaagtga agaagcacct agtgcagatg tatttgttgt tttgtatgaa ttagatacag
    13801 tgatgtactt atctttattg aaagtcaatt atcgtgaggc ttacactcac tttgttgaag
    13861 cagacgaagc agggatcgac aaccgcttga ttttgaatcg agcaattcta ggggcgaaaa
    13921 cacaaaaagc agatgaagca ttcgctgtga atttgactga ttggacctac gaattaattg
    13981 aaaaaaaata tgagttttct ggcaaaaaag agctttattt ttctggaaga gtgatcgaaa
    14041 gtgttcctgc accttcttta gaagaaaatg taaaagtgat ccaaaaagta gccaagcatc
    14101 ttggaaaaaa atttgagaca gaagaatttg atatcatggc tgatttgaaa gaagctgtct
    14161 atgatacgat agaagaaaaa ggtcggttgg atcatgagat gattgcggaa aaagtcttca
    14221 aggaaaacat cacggcaaaa atggctttta aagaagaagt acaagagcaa ggatttgtcc
    14281 cagaagcacc tcctgtaaaa gaagtacaag aaatttctga gaaaaaattt ggaaaacaaa
    14341 aattcaagct atcaaacggg atcgaactga tcgtaccagt agatgtatat cgcaacccag
    14401 atttgattga atttgtgaat aatccggatg gtacgatatc cgtgatgatc aaaaatgtgg
    14461 atgaagtatt gaatcgttta taaaaatgac tagaacatta ggtgttcaag taccagtgat
    14521 cagttaaatg aattgtgtca agagaaggat cggaaaagac gaatataaca aaaaaagagc
    14581 gtgagacaaa agtaaagaac acttttgtct cacgctctaa acacgtataa acggcggaag
    14641 cagaagcaac tccttcttat ttcttggagt taaacgcttc tgtcccagcc tctttttatc
    14701 taataaaatt ttaagaccat ttactaatta tcgatttttt ctgccgcatt tttaggcact
    14761 tcagaagcct ttatttcttc ccagcttaat acacctttac gaggattgac tttgagttta
    14821 agatacgcat tcatctttaa tggatgttct ctttcttcgc gaagcgtcac ctgttttttt
    14881 tcaccatctt tattataagc cgcttgcttg taggtataag tcgtgaattt ctctccgtta
    14941 tctgcttggg tagctgacat ttcgccattc gtcgtgattt ttgtataata agcttcgccg
    15001 ccgtaaaaat aatgataagc tgcaaaactt ccgccgagaa atatgcctaa aacgacaagt
    15061 acgccaatta attttttcat gtacttcacc tctttcctta ttattaacga aatagaagat
    15121 ttcgtctatt gaaagttgtt ggcaacagct gaattttaaa cttttcctca ttttgttctt
    15181 attgcttcaa aagaaagtta aggactatct tgaagttttt ctcaaaaaac acattttttt
    15241 atttataaaa ggtacttttt tcgcgtataa gacaagcgag tgctcttagt tgactttgac
    15301 caatattgac gtataataag cttagttcga atcttacaaa aagtaagtcg gacgtattct
    15361 ttttgattta gtcaaaaaaa gactataata aaaaggtgag agctgaaatt tttcagtgat
    15421 ctatattatt taggaggttt gtaaattatg gcaaaagaac attatgacag aagtaaacca
    15481 cacgttaaca ttggtacgat tggacacgtt gaccatggga aaactacttt aactgctgcg
    15541 atcactacag tattaggcaa aaaaggccta gcaaatcctc aagactatgc tagtattgat
    15601 gcagctccag aagaacgtga acgtgggatt acgatcaata ccgcacacgt agaatatgaa
    15661 acagaaaaac gccactacgc gcatattgat gcaccaggac acgcggacta tgtgaaaaac
    15721 atgattacag gggcagcaca aatggatggt gcaatcttag ttgtttctgc aactgacggt
    15781 ccgatgcctc aaacacgtga acacatttta ttgtcacgcc aagttggtgt aaaatacctg
    15841 attgttttct tgaacaaagt tgatttagtc gatgatgaag aattgatcga tttggtagaa
    15901 atggaagttc gcgagttatt gagcgaatat ggtttcccag gcgatgacac tcctgtgatc
    15961 aaaggttccg cattaaaagc attgcaaggc gatccagatg ctgaagctgc tattatggaa
    16021 ttgatggata cagtagatga atatatccca acaccagaac gtgatacaga taaaccatta
    16081 ctattgccag tggaagacgt cttctcaatt acaggtcgag gaactgttgc ctcaggtcgt
    16141 attgatcgtg gtgctgttcg tgtcggtgat gaggtagaga tcgtagggat caaacctgaa
    16201 acacaaaaag cagttgtaac aggtgtagaa atgttccgta aaacgttaga ttacggggaa
    16261 gctggggata acgtaggcgt gttgttacgg gggatccaac gtgacgatat cgaacgtgga
    16321 caagtgcttg ctaaaccagg ttccattact ccacatacaa aattcaaagc agaagtgtac
    16381 gtgttgacaa aagaagaagg tggacgtcat actccattct tcaacaacta tcgtccacaa
    16441 ttctacttcc gcacaactga tgttacagga acaatcacat tgccagaaga tacagaaatg
    16501 gtcatgcctg gtgacaacgt gacgattgat gttgacttga tccatccaat cgctgttgaa
    16561 aacggaacga cattctcaat tcgtgaaggc ggacggacag tgggttcagg tatcgttaca
    16621 gagatcgaag cataagaaac gcgactcgaa tattttggta atgaaaaatg tttaaaataa
    16681 aagagcggtc caaacaggat ttctgtttgg accgctcttt gttcttcaat tgtataagaa
    16741 gagattgtag aagcatgaac tttcgtttta cttcagtctc ttttattcat catgagagtg
    16801 ttcatctatc ggtatgcagt ttttaatact ttcttcgtat ccattttttc aaaccactta
    16861 ttctaccttg tgtctgctca actggcgcat tatcaataat cagcaaagca atcataacat
    16921 atgcaatcca actatctcta gaatatagag tataacgtga gacccagttt gtagcgtact
    16981 tgcttttata ttctcttaat ccatggaaag aataaaaatg agaaccaaac tcgtaaacca
    17041 agtatgcaat cctttcctgt aaaaaactct ttctggaagt tccgacattt gaaagagggg
    17101 ccatccctaa attgaaccat tggatatgtt cttctttcat ataggcaaac aaatgcaaga
    17161 acaagaaatc catactgcca gaaggtgctt ttttaggatc atatcgcatc agatcaatcg
    17221 ttcctacttt attgttcgtg taagtaggca tgatcgttgc aaaagcgaca atttcttctt
    17281 gggtattttt gacaactgca ataggcgctc tttgtagata atcttcagag aaaaagccta
    17341 aagaaaaccc tttttccttt cgtgaaccca accaattatc agagatattt ttgaaagtac
    17401 tcatttgctc agtagaaaat ggtggttgta agacatcaaa tgtaaatcct tctcgttcga
    17461 tccgattcaa gacagcacga gtgcccttca tttttttccc tgaagttgta aaactattca
    17521 ggtctacata agcttcttcc cccatcttaa tgaaatcgta gccgaattca tgcaaaatca
    17581 taacaatctc ttcgcttgtt tcgtagaaaa ctggtaaata gcatagtcga tctgtttctt
    17641 cgataaaagc ttcgattgct tctggaaaat cttctttctt accagaagga tcacccatta
    17701 caatacattt attattgaag caagcgaatt ggagaagaac agttggttca ccgtcttttt
    17761 cataggcaaa catccgctta tcttttaaga agatcaattg actatcggag tttcctccat
    17821 aagttgtaag gatcttcagg gccttttctt cattaaaggc ttcgccaatc tgtttctttt
    17881 ctccttgaag gaaatgaaca aatagaacga tcatgaaact aacagcgatg atggccaaca
    17941 atcccgaaaa ccaaatcttt tcagaaggaa ataagaaaaa ggaaataaaa tggtgacgcc
    18001 gatgagggaa atccggcaga ttgtataccc caataacgat gtaaagcaag gacagtgcac
    18061 caataatgat tccatcgatc gtacgccact cccatgaata gatcaattgt tcccgaaata
    18121 attcgttttt agaagcaaga atgatcagaa gcagaataga caagaaaacc accggtgtga
    18181 aactaaaatc acttaataaa acataaagta aagctagaac aatcaagaag attgtaggca
    18241 aatatgctcg tttcacacgt gctgcgatcc ctctgcccat aatgattaaa agaaatccca
    18301 atagtatact aggaaattgg ataataaaat gaaatttcaa tgggttcaaa ctatgaagcc
    18361 agcgaaactc tgtaaaagct tgcgggatag tggcagaaag tactagcatg attccagaaa
    18421 agtataataa gacaaccacg attttatgcg caatttctgt tgctaattgt ttagggatac
    18481 cagaataacg ctgatcaaaa gtggaaccga tatttttgaa gaaaaagacg attccaatca
    18541 aaaacggaat gatgtagtag aacaggcgat agagcaagat ccacacaacg actatttctc
    18601 ttggcacacc aatagcggac aacccgataa tcatcattac atcgaaactc ccaatttctc
    18661 ctggaatcat agacacgatt cctatcactg aagcagcaat gaataacgga attgtttgcc
    18721 acagatcaat ccggatttcc attaaataac caacagaaag gaaactaaac aggacaccgg
    18781 tccattccaa caatgaaact agcaatagct gtccttgagt tttaagtgag gtaccaccaa
    18841 ggaatccttt ctttttgaaa gtagtaaata aaaagacgac aggaaaatat agaccgccgc
    18901 cgatcaacca tatccaatat tgcttcaaaa aaggagcaac tggcgtgaag actacaagta
    18961 aaaaagaaag cagactgtaa atagaaagtc cagccatcaa aaacaagaaa actttcgata
    19021 atgcttggat cacgtttctg ctctctttct tttgtccata tagttcagag cgaagcccca
    19081 cactaacaaa cccgccgaaa ccggcaatat tgttgattgt attgatcagc cagcttgtct
    19141 caaacaagta acgaggattc tgcttttgtt caagcatccg attcaaaata atatcataac
    19201 caatcatagg taacacagag acaagaccaa tcaacagcat aagtccgctt ttccatacag
    19261 gtatagtggc aaacgtttct cctaattgct ggatagatag agttttaccg atggacatca
    19321 attcaccgac aataatgaca atgacagaaa tcaaaaagat tgttttgaac agtcctaggt
    19381 ggtttttcag ccattgtatg gtatttttta acaaataaat caccttctat tcctaatagt
    19441 tatgtttcag aaagaaattg aagaacttct cgattgaagc gttcaggatc ttttctagct
    19501 aattcatgtc cttgttcttt cacaagcaca aaggaagctt ttggtatagt tttagctata
    19561 tacaaagaat gttttagctt gattacatct ttttttccca cgatgatcaa agtgggggag
    19621 ttgatttttt ttaaatcatt ttctgtaagc ccgatgtcat gcagcaaaag tttgataact
    19681 aaaagatttt tgcgaagact tgggcgaaat aaagagagta accataccca tgcataatga
    19741 atattactga tgactctggc agaaaaacga acgcctttaa caagcgtgtt tccagaattt
    19801 aaaatcaatc gatgaacttt tttagggaag ctgctggcaa ataccagtgc tagatttgct
    19861 ccatcactaa atccaagaaa atctgcctga tcgatttttt ccagcaacat gatcgtattc
    19921 agatcctcgg ccattaactg aaaattcaac atagatgctt cgtttgtcga acgaccatgt
    19981 cctcggctat ctactagata cacggtatag tagcgttcga gtacagggac ctgttctgaa
    20041 aaaaagcggc cgctgccgtc attgccatgc agtaaaaaga gagggaagcc ttgaccgctc
    20101 ttttcatagt aaatttcgct cccatctggc atttttgcat atttcttttc ttttttcaca
    20161 taatcaccta ctgtgtaaag tataacgatt atatcagtca ttgggcaatt gtttttactg
    20221 aaaaacaacg atcattttcg taagatgaca aaaaatacag gaaaattcat tttttcttaa
    20281 ggcgtacaca gaaatcaagg aagaaatagc caagaagata aagaaatagt aagataaaaa
    20341 aagatgtcaa aataactagg tgatgaaatg aagcgaaaac gattatctat tatccgtatt
    20401 cttttttaca tgatcattct gacggttatt ggtgcggtgt tgtttttagg aggaagaact
    20461 tatcgttcgg taaaacgagt ggaaagctat caaatagaaa taacaacagc tgcaaaaaat
    20521 tatcatatgg aaaactacga gaatttgatc aaggcgataa tattgaccga atcaaaagga
    20581 aaagggacag atctcatgcg gagctcggaa agtctgcatg gagaagctga gatcattgat
    20641 aatccagctg acagtattga tcaaggtgtc aaatatttgg cggaaatgat agtagaagca
    20701 aagaaacaag gatgcgattt atggacagct gtccaagcat ataattttgg gaaagattat
    20761 attgcctatg taaaaaatca tggcggagaa aacacagtag aattagcaga agcgtattct
    20821 aaagatgtgc tgagcccact tttaggaaat gaggaaggga aacaataccg atattggcgg
    20881 atccagtcct tgctttacaa tggcggctat ctctatcata atggaggtaa catgttttat
    20941 cctgatattg tcaaaatgaa tgaaaaaata atagagacct atgaaaaagt ctttaagtaa
    21001 aatagcagga aatgttcaat catgaataaa attaaatgaa agaaggagtg cgcaaatagt
    21061 ggaaaaaagg caaaaaatgg aaaatcaaga agtggggtac acaaccaaag actggatgtt
    21121 acggtttgtg aagggaatgt ttatcggttc aggatttatc ttgccaggag tcagcggtgg
    21181 tgcattagca gctgtctttg gcatgtatga gcggctgatt tcttttttag cccaccttac
    21241 aaaaaatttc aaagaaaatg ttttgttttt tctgcctgtt ggtctaggcg gagtgacagg
    21301 catctttcta ctttcttttt tagtcagttt tttattaggt gcgtatgaaa caacgatatt
    21361 atggtttttt gtcggatgta tcgctggaac tgttcctgcg ttatggaaag aagcagggaa
    21421 aaaaggaaga gatacttctg atgtgatcat catggttgtt acttttgttc tggcttattt
    21481 gtttttatta tacggcgctc aattatttga cgggcaagtc gatcagaatt ttggaacatg
    21541 gctgattgcc ggcggattga ttggtttggg tatgatcgtc ccaggattga gcccttctaa
    21601 ttttttggtg tatatgggat tgtataaagc gatggctgac ggatttaaag aagtacgact
    21661 tgaagtgatt gtgccgatcg gaattggtgg gattctttgt gttcttggat tgtcaaaaat
    21721 catggattac attttctcaa aggcatatag caagctattc catttcattt taggtgtagt
    21781 ttttgcttca acgattatga tcattcctac cgattacgcg ggattgggag ttgctggggt
    21841 actagcttgt cctgttcttt ttatagccgg tacagcacta ggttggtgga tgagccgttt
    21901 ggaggaacaa tataagtaaa gatagattga taataaaaac agcacacggg tgattcaaac
    21961 aaaaaggttg gcatcttttt tgtaaaatca ttcgtgtgtt tttctataaa ttttataaaa
    22021 acaagttgtt tttaatcttg gaacacgcta gacttaaaca cgtgattatt tttgaaagtt
    22081 ggcggaaaat ggagaaaaag aaaatgaatg gacttacaca gcaagaggta gttcagaaaa
    22141 tggcttctgg ccagcaaaat gactaccagg aagatgcatc aaaaagtaca aaacaaatat
    22201 tcagcgataa tatattgacg ttattcaatt ttctgaactt tggtattggg atttgtctgt
    22261 tatttgtagg tgcgtattcc aacatggcgt atttagcaat tattgtcgtc aatatcgtta
    22321 ttggaattta tcaagaaata catgcaagaa atcttgtacg gaaattatcg attgtttccc
    22381 aatcagaagt actagtgatc cgggacggta aagaacaatc gatttctact aaagagctgg
    22441 tattaggaga tttggtaaaa atcggagcag gagaacagat accagccgat ttacaggtta
    22501 tatctggacg ggcagaagca aatgaagctc ttttgacagg cgaatctgat ttgattgtca
    22561 aagaaactgg agctgaattg ctttctggaa gctttttgac aagcggttct gtcatggctg
    22621 aagttatccg agtaggtgca gataattatg ctgtccgctt gactagcgag gcaaagactc
    22681 acaaagctat tcgttctgaa cttgtagatt cgattcgcaa agtatccaaa ttcactagtt
    22741 tcgtgattat tcctttgggg atcatactat ttttggaagc actgttcatc cgcgatgctt
    22801 ctatcaaaat ttctgttatt gcatctgcag cagctttgtt aggcatgctt ccaaagggtt
    22861 tggtattgct aataagtatt gcattaacga caggggtaac caaactggca aaaaaacgaa
    22921 tccttgttca agacatgtat tcaattgaaa cgctggccca tgtcgataca ttatgcttgg
    22981 ataaaacagg gaccattact gaagggaaaa tgagtatcca gcgggtagaa gcactacatc
    23041 cgctgtacga gaaagccatc ccagaaatta tggggacgta tctggcagaa agcagtgata
    23101 acaacatcac tatgcgggct atgagagatt attttgagct ttctaatcgg tatgatgtga
    23161 caaacaaagt cgcattttct tctgatcgca aatggggcgc gatggaattt ccagaactgg
    23221 gagtcgtgta cgtaggagcg cctgaacgtt tggtggaaga agatcggctt ccggacagtc
    23281 taaaaacagc ccaagcgaat ggctatcgcg tacttatgtt ggggatcgct ccaaatcagc
    23341 aattgaacga agaaaaacca gcatttgttg agccggttgc tctgtttgaa atcgatgatc
    23401 caatccgaaa aaacgctcaa gaaacactag cttatcttca tgaggaaggt gtggatctga
    23461 aagtgatttc tggagacaat ccttttactg tctccaatat cgctagacgt gcaggattac
    23521 cgaattatga tgcgtatgtc gatctttctc aaattactga agaaatggaa gtgcgagaag
    23581 ctgctcatcg ctatacggtt tttggacgag tgagtccgca gcaaaagaaa ttgctggtaa
    23641 atgaattgaa agaaagcggc agaacggttg caatgacagg cgacggtgtg aatgatgttt
    23701 tggcgttaag agaagcagat tgcagcattg ctatggcaga aggtgatgga gcaacacgtc
    23761 agattgcgaa tctggtattg ttggactctg attttactac attgccagaa gtcctgtttg
    23821 aaggacgccg agtggtcaac aatgtgacga aagtctcagg catctttttt atcaaaacga
    23881 tttattcatt tattttatcg attatatgtg ccgttacagc aattggattt ccgtttatcc
    23941 ctatccaaat cacgctgatt gatttagcga ttgaagggta tccttccttc tttttatctt
    24001 ttgaacaaga taaaagaaaa atcacttacc gttatttgcc tacagcactt attaatgctt
    24061 taccaaatgc cttgcttgta gtagtgaata tcattgcggt gtatctactt ggaaaaacat
    24121 tcgcatgggg aaatgctgaa actactactt tgatgtatta cttgctgatt gggatcagtt
    24181 gcatggcagt tgtaaaagct tgttatccct tcaacccatt gcgcatcttc ctagcagtaa
    24241 cgacgatcgt agggatatat gtagctgcaa tgctgttcca tggaattttg gaagtaaact
    24301 tagggttggg agagagcgta ggatacttct ttgtattcat gctcgtaaat attgttcttc
    24361 gagtattgta ctggaaggct gatattcaaa atcagttact gaaaagagta gcaaattgat
    24421 gcttaatgag atagaaatgt gttatacttg aaaaagcaat gaaatatgat acaatcaaac
    24481 atgtataatt gtgtccttta aaatcgcact gatttcagtt agtgcgattt tttaccggtt
    24541 attctcctga aatatcttct gttaaaccca aaagatatca atagagcagg gggtgaaaaa
    24601 atggctcaac ggattcctca agaggttatc gaaacagtac gcagtcaaac gaatatcgtt
    24661 gaggtgattg gacaatatgt ccagttgaaa aagtctggta aaaattactt ggggctgtgt
    24721 ccttttcatg aagaacgtac accgtctttt tctgttgcag aagacaagca gatttttcat
    24781 tgctttggtt gtggtaaagg cggaaacgtg tttacttttc ttcaagaact agaaggtcta
    24841 agttttccag aagcagtggt aaaagtggca gacttcgaac agataccgtt agaagatcaa
    24901 tggcgtcagc gtactatagc tgtccaaaat cctgaatctg ctacggggaa actgattgct
    24961 gctcatgaaa aagcagcgga aatctatcat cacatgctat tgcatacaaa agctggacag
    25021 ccagctttag aatatttgca aagtcgtggg ttgtctttag aattaattga agaattcaac
    25081 ataggttttg cacctaatga gcgttctttt cttcagcaag tattcaagaa tgaatcaatt
    25141 tcaaatgaat tgttagaacg ctctggattg tttgtcacgc atgatgatgg ccgtatgtct
    25201 gatcgttttt atcaaagagt gatgtttcca cttcgtaatt ctcaaggcca gacgatcggt
    25261 ttttcaggac gctggcttgc acctgaaaaa gggcaggaaa aagatcagcc caaatacttg
    25321 aatagtccag aaacagaact gttcaataaa agacaagttt tatttaatct tgataaagca
    25381 agaagcgata tccggaaaaa cggagaaatt ttgttgttcg aagggtttat ggatgtgatt
    25441 gctgcatggc aggctggtat caaaactgga ttagcttcta tggggacaag tctaacagct
    25501 caacagatcc aacaaatgga aaagctgacg aaagaattgg tcttttgcta tgacggagac
    25561 aacgcaggtt ttgaagcaac caatcgaggg attgaattgc ttcggcaaaa cagtcgatta
    25621 gcactttctg tcgtagtcgt tccagaaaag cttgatcctg atgaatatct ccgtaagtat
    25681 ggagaagcat ctttccaaga attagtggct catgggcgcg aaacagtctt tacttttaaa
    25741 tcacgttatt taaaacaagg gaaaaattta gataatgaaa aagacaagat cgaatacctc
    25801 gaagaattac tagttgaact tagccgagtt gtttcaccaa tcgaacaaga tatgtatcta
    25861 tctcaactgt caacagagtt tcagatcggc agagagacga tacaaaaaca actgcgcgat
    25921 cttcgaagga caaatcagca aaatcgtcaa gaacctcagg aaagtcaaca cactcaggtt
    25981 acgagacaag aaactgcacg gaaaaagaga cctttgacgc aagtagaaaa ggcagagcag
    26041 attctattat atcgtgcatt taaagaagtt tctgtccgta atattttaaa agaacgagaa
    26101 tttcagttta ttcatgatgc atatgcagaa gtttattttt tattcgatgc atatgtctca
    26161 caacattatg aattcaacct tgcagagttt ttagactttt tgcaagatga aaatttaagg
    26221 cggttggtgg tggaaatcga atatttaccg gtggcagaag aaagttcgcc acgagaaatc
    26281 caagacttgc tagatgtgat cagcaaatct ggcttagctg atgaaataac attaaaaaaa
    26341 caaatgcagc aagaagccag acgaactgga aacaaacagc ttgagcttga attgacgatt
    26401 gaaatcatca atttaacgaa acagttaaaa caagccaaat agctactcaa ggggggcctt
    26461 cttttatggc aaatggaaca gataaaaaaa cgtatgaagc agcagtagca gctttcatca
    26521 aagaaaacaa acccaaacga caagttgtat atgatgattt atccaatcag cttgctacac
    26581 cattcacttt gaatgcagat gaaatggaca aattgatcca aaagattgaa gacgcaggta
    26641 tcagtgtggt ggatgaaaat ggtgatccat caattcacag cttgaagagt gctgagaaaa
    26701 aagctgaaca agcaaaaaca gaagatcttt ctgcgccaac cggtgtaaaa atcaatgacc
    26761 cggttcgtat gtatctgaaa gaaattggcc gtgtctccct tttaactgcg gaagaagaag
    26821 tagcgttagc attgaaaatc gaagaaggcg accaagaagc gaaacagcgt ctggcagaag
    26881 caaaccttcg tttagtagta agtatcgcta aacgttatgt aggacgaggc atgcagttcc
    26941 ttgacttgat ccaagaagga aacatgggac tgatgaaggc agttgaaaaa tttgactacc
    27001 gcaaaggatt taagttttca acctatgcta catggtggat tcgtcaagcc atcactcgag
    27061 caatcgctga ccaagcacgt acgatccgta ttccagttca tatggttgaa acaattaata
    27121 aattgatacg tatccaacgt caattgctgc aagaccttgg aagagaacct acgccagaag
    27181 aaatcggtgc ggaaatggat cttccaacag aaaaagtccg agaaatcttg aaaatcgcgc
    27241 aagaacctgt ttcccttgaa acgccaatcg gtgaagaaga tgactctcac ttaggtgatt
    27301 tcatagaaga ccaggaagca acaagtccag cagaacacgc agcttatgag ttactaaaag
    27361 aacaattgga agatgtacta gatacattga ctgatcggga agagaatgtc cttcgcttac
    27421 gttttggttt agatgatggc cggacgagaa cattggaaga agtagggaaa gtgtttggtg
    27481 ttactcggga acgtattcgt cagatcgagg ctaaagcatt aagaaaattg cgccatcctt
    27541 ctcgttcaaa acaattaaaa gatttcttag aataagcgtg gactattgac cagaagagaa
    27601 atcagtaaaa ctgtttggtt tattttaaaa gaaaaaaagg gctctttgtc aataaggact
    27661 gatgagttgt gcaaaatcaa atctgagtca gaatgaacc
//
LOCUS       DAZKKW010000031        27520 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.031, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000031 DAZKKW010000000
VERSION     DAZKKW010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27520
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.031"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(339..452)
                     /locus_tag="ACG8PO_001287"
                     /pseudo
     CDS             complement(339..452)
                     /locus_tag="ACG8PO_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(835..2067)
                     /locus_tag="ACG8PO_001288"
     CDS             complement(835..2067)
                     /locus_tag="ACG8PO_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFQ0206627.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            2322..2891
                     /locus_tag="ACG8PO_001289"
     CDS             2322..2891
                     /locus_tag="ACG8PO_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0206628.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(3069..3509)
                     /locus_tag="ACG8PO_001290"
     CDS             complement(3069..3509)
                     /locus_tag="ACG8PO_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HFQ0206629.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            3667..4431
                     /gene="map"
                     /locus_tag="ACG8PO_001291"
     CDS             3667..4431
                     /gene="map"
                     /locus_tag="ACG8PO_001291"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFQ0206630.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            4463..5386
                     /locus_tag="ACG8PO_001292"
     CDS             4463..5386
                     /locus_tag="ACG8PO_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HFQ0206631.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            5462..6601
                     /locus_tag="ACG8PO_001293"
     CDS             5462..6601
                     /locus_tag="ACG8PO_001293"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HFQ0206632.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            6594..7394
                     /locus_tag="ACG8PO_001294"
     CDS             6594..7394
                     /locus_tag="ACG8PO_001294"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFQ0206633.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            7394..8221
                     /locus_tag="ACG8PO_001295"
     CDS             7394..8221
                     /locus_tag="ACG8PO_001295"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFQ0206634.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            8226..8933
                     /locus_tag="ACG8PO_001296"
     CDS             8226..8933
                     /locus_tag="ACG8PO_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFQ0206635.1"
                     /translation="MRSVCIATFNGGRYLREQLDSILSQLSAEDEVVISDDGSVDDTW
                     DILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWLETKVKKITD
                     YFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYIGAGMAFRSS
                     LKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIETHSKLRQKIS
                     WRVHLITALIKRKWFKR"
     gene            9033..9899
                     /gene="rfbA"
                     /locus_tag="ACG8PO_001297"
     CDS             9033..9899
                     /gene="rfbA"
                     /locus_tag="ACG8PO_001297"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFQ0206636.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            9913..10485
                     /gene="rfbC"
                     /locus_tag="ACG8PO_001298"
     CDS             9913..10485
                     /gene="rfbC"
                     /locus_tag="ACG8PO_001298"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HFQ0206637.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            10507..11535
                     /gene="rfbB"
                     /locus_tag="ACG8PO_001299"
     CDS             10507..11535
                     /gene="rfbB"
                     /locus_tag="ACG8PO_001299"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFQ0206638.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTSILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWQPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            11633..12484
                     /gene="rfbD"
                     /locus_tag="ACG8PO_001300"
     CDS             11633..12484
                     /gene="rfbD"
                     /locus_tag="ACG8PO_001300"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HFQ0206639.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLPYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEALGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            12519..14552
                     /locus_tag="ACG8PO_001301"
     CDS             12519..14552
                     /locus_tag="ACG8PO_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0206640.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFSLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMIAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFVALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            14596..15876
                     /locus_tag="ACG8PO_001302"
     CDS             14596..15876
                     /locus_tag="ACG8PO_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HFQ0206641.1"
                     /translation="MKIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGVGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFTVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNTIGSSLMTLALLISGFASSFERWYVRIIGGIAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTGREDIWHKFY
                     QTLGEKSEQVLLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYRWVPIIYILLAMAVHRYDEPEVTEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            16086..16892
                     /locus_tag="ACG8PO_001303"
     CDS             16086..16892
                     /locus_tag="ACG8PO_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0206642.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            16904..18124
                     /locus_tag="ACG8PO_001304"
     CDS             16904..18124
                     /locus_tag="ACG8PO_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0206643.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            18114..19700
                     /locus_tag="ACG8PO_001305"
     CDS             18114..19700
                     /locus_tag="ACG8PO_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFQ0206644.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAEPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSETT
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKELPEFVLTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSREEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            19739..21877
                     /locus_tag="ACG8PO_001306"
     CDS             19739..21877
                     /locus_tag="ACG8PO_001306"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFQ0206645.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRKENGHISLATNSALEIAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDVIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYSNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTVMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKEGIGDFSLRPD"
     gene            complement(22245..23228)
                     /locus_tag="ACG8PO_001307"
     CDS             complement(22245..23228)
                     /locus_tag="ACG8PO_001307"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFQ0206646.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
     gene            23598..24989
                     /locus_tag="ACG8PO_001308"
     CDS             23598..24989
                     /locus_tag="ACG8PO_001308"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFQ0206647.1"
                     /translation="MNKNGEWSAARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNL
                     ETFQHSAIIISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRW
                     FAFPRLVILLSFVLGTLFLSIYRILVYKLYLRVSRDKKVVIIGLEKDVAPAIQNFGSK
                     KNNKHKVEAVILDNYYANIKNMIKDIDIVYLASHIEETEKIKIYQLVTKNEKKLFLNT
                     TFENLTLINPNIMNFEDESIIETSGFRIQPEYELFKRLFDIIVSLVLLVIASPFMLFT
                     AILVKVTSPGPIIYKQVRITKNQKEFSIYKFRSMTATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNAENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLMLDLKILLQTIKILFDKMSSQGLEEDENVIS
                     LAEIAFPNEKIFR"
     gene            25005..26045
                     /locus_tag="ACG8PO_001309"
     CDS             25005..26045
                     /locus_tag="ACG8PO_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFQ0206648.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCEDRIFNLHYYEYCHQVIMNPKVEYIYEFE
                     GGKGITNQYRPNKFETFKEFYSLANTVTDDINKDGMAALLLKGTTSVIFSIYSTSART
                     SKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNMPAPLFLSAVKVGSFVEVK
                     MPGLMAALKRVY"
     gene            26064..27149
                     /locus_tag="ACG8PO_001310"
     CDS             26064..27149
                     /locus_tag="ACG8PO_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HFQ0206649.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYHSEIERLGGRIFVVPNPKKNIFKYLIEVNKLLKKESFSIVHNQVYFGGGINLWL
                     AKKNGIRQRIAHSHATEDGKSQNIVMNVLRKFLTKLLLQNATDYLAVSQEAGESLFQN
                     HPFEIVHNGIDLELYSKNSEAKVNKRKELDISMSTFVVGNIGRLEKQKNQSYLLEIFL
                     EILKMNSDSYLILVGEGSLRNKLKAKAVELDIEKRVKFLGERNDIPELLSVMDVFVMP
                     SLYEGLPMVGLEAQAAKLKLVLADTISKDTKLTNNVDFVSLDETPQEWAKKVIKEPYA
                     NKITQELKSYDVSYTTEQMESIYSRDH"
     gene            27182..>27520
                     /locus_tag="ACG8PO_001311"
     CDS             27182..>27520
                     /locus_tag="ACG8PO_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-1,6-N-acetylglucosaminyltransferase"
                     /protein_id="HFQ0206650.1"
                     /translation="MKICYLILAYNDIGNLKRLVNRLNENAVFIIHIDKKTDIEPFIE
                     AFKSYDNVEFLCNKNRVKIYWGGFSIVQAEINLVKRALQNEKYLKYVLLSGADYPIKD
                     NEYIYNYFKKN"
BASE COUNT         8857 a         4571 c         5556 g         8536 t
ORIGIN      
        1 ccatacttta taaaaatctt tgataaatct attcgaaggg tcaaatttaa accataattg
       61 tgtttttcct tccatcagca taaaagttca cctcaaaaag agtatacgaa cgaatgttct
      121 ttttgtgaag gcggaattta atatactgaa taatagaaaa caagtacttg tgccaaatta
      181 tgtgccaaaa aaattcgaat tcaacaaaat gtaaacaaat agaaaacctt ctatattaag
      241 atttcctgca ataacaaaca caacagaaga cgtgtaatag ttagtcagga acgtacaaat
      301 aacccctgta tcctttgcgg tacagggggt tattttgtat aagtagacat tgtagtttct
      361 aaactgctat gtcctaaacg tttcgatacg ctaagtatat ttactccttg ataaagtaaa
      421 atagatgcat gcgtatgtct tagtccatca ataaacagtc gaagagaaaa tagggaaaaa
      481 ttgattacta aagaaaaact tactaggtcg acggtgtagt tgattttcat acttcaatag
      541 attcgaatta aacgatttcg attggcttat accagtccta tccaacgaaa gatatatcgt
      601 tcttaactct agcggtataa cttgaagctt tacgtaaagc caatgggaat gaactggaga
      661 tgacttttta ttatttttag taatagctga tgggttaata cttacaactt tccttttcaa
      721 agtaccatat aaaaaaacgg tggggttgtt tagcgaaata aaggatctaa gccaatagct
      781 tagatccttt attctatgct ttatattatt ttttgcatgt gtgcgaaagt atactcaagc
      841 ccaatcgcca tttcggaaaa tcggtactgt agagccatct tcacggattc cgtcgatatc
      901 catcttgtca gatcctacca tgaaatctac gtgggtttga cttctattca atccagcttc
      961 tgctaattct tcatcggaca tttctgtacc gcctttcaca ctgaaggcat aagcagaacc
     1021 tagtgctaag tgattcgatg cgttttcatc aaataatgta ttgaaaaaga tgatgccaga
     1081 ttgagaaata ggtgaagggt caggtacaag tgcgacttct cctaaacgac gcgcaccttc
     1141 atctgtatca agcagttttg ctaagacatc ttctccttgt tcggcagaaa aatcaacaac
     1201 ttttccatct ttaaaagtaa atttcatacc cgagatgatg gtgccagcat aactcagtgg
     1261 ctttgtactt gaaatgtaac catctactcg atgactgtca ggtgctgtga acacttcttc
     1321 cgtcggcata tttgccatga atttttctcc acgagcatta tagcttccag cgccttccca
     1381 aagatggttt ttaggcaatc cgataatgat atctgtaccg ggagcagtat agtgaagggc
     1441 agaaaattgt tctcgattca attcttctgc tttttttgct aatttttcat catgcttctt
     1501 ccatgctaag acagggtctt cttcatagac acgagtcgtt ttgaaaatct gatcccataa
     1561 tgcctcaact tgtttttctt ctggcagttc tgggaagact tttgctgccc attgtttacc
     1621 agccgcagcc acgacagtcc aactgacttt atttgcttga gttgccttgc gtaaattcat
     1681 tagtgctttg ccattcgctg attgataaga cgctactcga tggctatcta caccagcaaa
     1741 tgcatcggga tcggcagaaa caacgctgat cctgcttgct cctttttcaa gccattcatc
     1801 cgcttgatcg attttagatt gtggtacatt ctcaatacga tccgttgcag catgtaaaag
     1861 aaattctctt tggatctggt cgtcagtcca ttgaacgata acctcagcgg ctcctagttt
     1921 ataagcttct tgtgtgatca atcgagcaag gggtgcctgt tccacgctga tctgtaaaac
     1981 aactgtgtgt cctttttctg tggctacgcc agtttctgca attaagcgtg catatttttt
     2041 tagaagttca ttaaaatcag ataacataat aaaaattcct cctcaccatt ttttctggta
     2101 actattagca tgataccatt taattatgat tcaaacaatg aggctttaaa taaaaaaagg
     2161 aggttatttt tttaaggtta agtttcatga tatatgttac tacttgctat ttcagcttgt
     2221 gaagaaaatc aaaaactaat tttgcatatc aaaaaaaaca tgttacaatg aaattaatca
     2281 atggtggaag cgttataaaa aaattaagga gattaaaaaa tatggcaaga aaaaagacga
     2341 ttactagaga acagattttg gcagctgctt atgaagtagc tgccaccgat ggatttacac
     2401 gatttactgc gagaaacatc gcgaacaaaa tgaattgttc cacgcaaccg atttatcttg
     2461 agtttaaaaa tatggatgac ttgagacaag ctctttttga gcagatctat gatcatttac
     2521 aatatgaagt gttcactgtt gagcatacag ggaatacgat tatcgatctt gcttttaatt
     2581 atatccattt tgcaagaaga gagaagaagt tgtatcgttc actgtactta gaagaatatg
     2641 gtggaggaaa agagatgcag aatttctctt atacttactt tgtgaataca gtgaaacgcg
     2701 atccagctta tgtgaatctt tcagaagagc agatcgaatc tttgtataat gggacatgga
     2761 tcatagctac tgggatcgca gctttgatgt cttcaagcat catccatcca actgatgaac
     2821 agattgaaaa gatgatccaa gacagtatag atgctatctt gaaattcaaa gaaccaatcg
     2881 aaatcgattg aatatactca aaagttccac aaatcagtag gtaacaagag atgtgtcaaa
     2941 ataaggaaaa gttcgttttt cttacagcgt atgcattttt aactcccgaa gaactcttcc
     3001 atgaatgcaa tgaataaaag agccatacag attgaataaa tctggatggc tcttttattg
     3061 caagtatatt aaatttttgc gactagttct ttagcgaaag cttctaaatt ttcgatatct
     3121 tcttcttcag cattcagatc cactttaaca ttttcggctc cttttgatgc gcctgttttt
     3181 gcaaaagcag catcgaaatc atccaccgat ttgcagaatt catcgtaaaa agtatcacca
     3241 gatccgcaga caccgtaaat ttttcccaat agatccaatt cttgcaagtc ttcataaaag
     3301 tctacgatct catcaggcag atcgccatca tcgtatgtat aagtagctac tacgcaaata
     3361 tctgcctcct cgaattcatc tgcatctact tgggtacatt cgttgatttc gacttcgata
     3421 tccagatttt caaatgcttc tgcaacaata tcggcgattt cttcagtgtt tcctgtcatg
     3481 ctagcatata cgatttttgc taaagccatg atgtgcctcc tcgtttataa ttatttaaca
     3541 attatgaatt ataccaaaac atcattgaaa agaaaataaa acctaaagac acctggggaa
     3601 tgtcatttgg ctaaatgtat gccgctatgt taaaatgaga gggatttgta aaaaggagac
     3661 gaaaatatga ttactttaaa atcagcacgt gaaattgaaa tgatggcaga atctggagct
     3721 ttattagcag atgtacataa aaatctgcgt gattttataa aaccaggaat cacaagttgg
     3781 gatattgagg tatttgtcag aaactatatc gagagtcatg gcggaattgc tgcgcaaatt
     3841 ggctttgaag ggtatgaata tgcgacttgt atcagtataa atgatgaaat ttgtcatgga
     3901 ttccctcgta aaaagccttt gaaaaacggt gatttggtaa aagtcgatat gtgtatcgat
     3961 ttgaaaggcg gcatttctga ttcttgttgg gcctatgtag ttggagaatc tacacctgag
     4021 atcgaccgat tgatggaagt aacgaagaaa gctttgtaca tcggtattga gcaagcacaa
     4081 gtagggaatc ggattggtga catcggtcat gctatccaaa cttatgtaga aggcgaaaat
     4141 ctagcgatcg ttcgtgattt tattggtcat ggcgttggtc ctacgattca tgaagaacca
     4201 gcagttcctc attacggaga agctggaaaa gggttgcggt tgaaagaagg catggttatc
     4261 acgatcgaac ctatggttaa taccggaact tggaaaatga aaatggatcc taacggctgg
     4321 actgcgtata caagagacgg cggactttct tgccaatatg aacatacatt ggctattact
     4381 aaagatggtc caagaatttt gacatcacaa ggagaagaag gcacttattg atctatccag
     4441 tcaataggta cgggagaggt acatgaaagt ttggaacaaa ataaaacaaa atgaaaatct
     4501 gatgcgcttt attgagacga ctcaaaagcg catcactgat tcagaaatga gtacgacctc
     4561 tgtcgtcgtt gcttattatt tactcttgtc tttatttcca ttgattattg cttttggtaa
     4621 tattttaccg tttctccaca tcgatcaaga gactgtactg acctatttac gagaagtgat
     4681 tcctgagacc gtttaccagt ttatcggtcc tgcaatcaaa gaccttttga cacaaagttc
     4741 aggaggcttg ttatccgttt ctgcattagc ggctttatgg tctgcaagtc aaagtatcaa
     4801 tgctttgcaa attgcgatga ataaagcgta tggcgtagaa aatagaaaaa attttttgat
     4861 cgtccgtttc ttctcactaa ttgtcattgt cttgttcatg attgctatta gtggggtaac
     4921 tatcgttctt gggttgggac agctgatttt ggatgctatt cagccaatcg tacaaatacc
     4981 cgtgaacttt atcgatcagt tccaaacttt gaagtggcca atcacattgg tagctttatt
     5041 tgtcatcatg ttcttgattt atttaattgt ccctaatgct cagttgaagt tgaagacgat
     5101 cattccgggc acgatcttcg ctactgtcgg ctggatgctg ttatcacaag tgtttagtat
     5161 ctatgctagg tattttgcaa cgaggatcag cggttatcag atcataggaa gttttattgt
     5221 actcatgctt tggcttaatt ttgctgctac gatcatcatt ttgggcggaa tcatcaatgc
     5281 agttgttcaa gaatacgtga cgggtcatca aatcaaaaaa cgtcgagatc caagccgaaa
     5341 atggttcaat aaattgaaaa ctaaattttc tcataaaaaa aagtagaaac aagagttctt
     5401 attgtaaatt tatatcagta atttataata attatgactc ttaattaaag taggtgccca
     5461 tatgcttgtt tcttttagta tggttataag attttttctg acaattttat tatcactgat
     5521 attaacacct gtattcaaaa tcatttcagt acaaacaggt atggtcgaca aaccaaatga
     5581 aagaagaata aataaagttc caatgccttc agcgggcgga ctgcctatct ttatttcatt
     5641 tgttatatcg actttattct tattccgtaa aattatccct caatcgtata ttgtaccgat
     5701 cttgattgct tctttagtaa tcatagtcac aggagtgctc gatgataaat atgagctgac
     5761 acctaaacaa aaaagtatag ggatcttgat tgcttctctt attatttatt ttgtagcaga
     5821 tatacgtatt gattccttca cgcttccttt tattggatat atccaactag gctggcttag
     5881 ttttcccgta acgatttttt ggatcttcgg tatcacgaat gcggtcaatc tgattgacgg
     5941 tttggatggg ctggcggcag ggatttcatt gatcggttta attacgatag gaatcattgg
     6001 atattttttc ctccatgctt ctacagtgta tattccagtt gttattttct gtcttgtagc
     6061 aagtattatc ggtttttttc cttacaattt ttatccggca aagatttatt taggcgatac
     6121 aggagctcta ttcctagggt ttatgatggc cgtcctttca ctacaagggc tgaagaacgt
     6181 gacttttgtt tcatcgattt ctttgctgat cgtcatggga gtgccggtaa cagacacgtt
     6241 ttttgctatc attaggagaa aagcaaaccg tgtttctttt tctacagcag ataaaaaaca
     6301 tcttcatcac cgactaatca gtttaggctt tactcataaa ggtgctgtcc tgacgattta
     6361 tgcgatggct ttgatgtttt ctttcacagc tatggtcatg aattatactg gaagaatcgg
     6421 aacgatcata ttgattattg ccatgttgtt tgctgcaatt ttattatttg aattgattgg
     6481 tctgatcaat gagaaacatc agtttatttt aaaaacattg cgatttttag ggaataaaga
     6541 gtaccgtacg caagtaaaac agaaatattt gaagaaatgg aaccgtcata tagatgaata
     6601 acaaattaaa ccagccgatc cttattagta ttgtgacggc aaacagtgat aaaatttttc
     6661 ggacactaga taattttatt tcaactgtaa aagatgaaac agcagtaaaa atccgaattt
     6721 ttgacaacaa ttcacttccc gagtacaaaa aaagactaac agagtatagg aaatatccct
     6781 acatcgatat ctattttcat gatgaaaatt ccggatttgg ttatggacat aatcacaatc
     6841 tgatagctag cgattctcgc tatggcatta tttgtaaccc agatatttta gtggatgaag
     6901 agacagttac agcattggtc ttgttattga aagaacatcc ggaatgtgcg atgttagcac
     6961 ctaagattct taatgaagat ggaacaactc agcacctcat ccgtgagcag ctggctgttt
     7021 ttgattacgt tttgcgcttt gtgcctttca aatgggtcaa aaaaaagttt gacaaacgac
     7081 tggctgtgtt tgaatgccga ggattgccta ctgatgctaa tagttatgta cggatgatct
     7141 caggtagttt tatggtagct gatattgata aatttaaaga gataaagggg tttgatgagc
     7201 gtttcttcat gtactttgaa gacaacgact tgtgtttgag ttttgaaaaa gcaggttaca
     7261 agttattgta cacacccttt tatgcagtta tccattttta cgggaagggt gcgcatcgaa
     7321 atgcaaaact gtttcgcgtg tttttgactt ctatgatgaa atttttcaat aagtgggggt
     7381 ggcgtttttt ctgatgaaac cagaaatatt agtagtcgtt gtgctttatc agcaactatt
     7441 cagtcaatct ccagcttatg gaccattaag caaagcactt agcgaaaagc aaattcagct
     7501 tattatttac gataatagtc ctcaaaggca gcaaaatgag ctgtttgaaa aagaaaatat
     7561 cgtttatcat catgatccgc aaaatcctgg tttagcggtg gcttataatt ttgcgttgtc
     7621 tcaagcagat gaaggaacac gttgtctcgt cactctggat caggatacga ggctagtcga
     7681 cgattatttt gaactattgc gtaaagtaac ctttacagat gaatgtgttg ctgcagttcc
     7741 gatgattttt tctgggagcc gacaagtttc tcccctatat tcagatcgtt atatcaatcg
     7801 tacagctcaa gcagtagaag ttgaaggccc aacagctgaa cgaatcatgg caatcaattc
     7861 aggtgttgcc tggtctgtca aatttctaaa aaagattggc gggtttaatc ctgctttttc
     7921 tttagatttt ttggatcatt ggctgttttg gaaagtggat cagttggaaa aaactgtgga
     7981 gatccttcct gtacgaatgg aacatgatct ttcggtttta gactatgaaa aagtttcaag
     8041 taatcgatac cgttctattt tacaagcaga aaatcgattt tatcatgaat atgatcaaag
     8101 gcatttatcc agtcaccgac gacaattatt gttaagaaca gctaaacagt tcttgaccgt
     8161 caaaaaccgg catatctggc ggatgacttt gcgctcatat gttaagaatt ggaaggtgtg
     8221 aattcgtgcg ttcggtttgt atagctacct ttaatggagg cagatattta agagagcagt
     8281 tagatagtat tttatctcag ctttcagcag aagatgaagt agtcatttct gatgatgggt
     8341 cagtagatga tacatgggat atattaactg aatacgcaga aaaagataag cgaatccagc
     8401 ttttttcggg gcctcgtcaa ggattgatcg ctaattttgg ttatgcgatc cagcagacga
     8461 aaggagatct aatctttttg gcagaccaag atgatgtttg gttggagaca aaagtgaaaa
     8521 agatcactga ctattttgag cagaatccgg atcagctagt agtagtttct gatttaatca
     8581 ttgtggacag cgagctgcaa gagatccatg attcgtattt ttcgtttcga aattccaaag
     8641 aaggatggct taataatgtg ataagaagtc actatattgg tgctggtatg gctttccggt
     8701 cttctttaaa acccgatatt ttgccgatcc cgtcagaagt gccgatgcat gatatgtgga
     8761 ttggtttgct gggaggaaag aagactgggt tcctaagaga accgttaact ctgtaccgaa
     8821 gacatgattt aaacgcgagt gagattgaaa cacattctaa attacggcaa aaaatttctt
     8881 ggcgtgttca tctgattacg gcgttgatca aacgaaaatg gtttaaacgt tgataaaaga
     8941 tgtttaaatc cttaatattt ccatgacaat tataacaatt gatgagataa taacaaagtg
     9001 aaatttgaaa tcaaaaagga gagcagatat ccatgaaggg aatcattctt gctgggggca
     9061 gcggaacacg tctgtaccct ttaacaaagg caacatcgaa acaattgatg ccgatttatg
     9121 acaaaccaat gatttattat ccaatgtcta ctctgatgtt ggcgggaatc aaagaaattc
     9181 tgattatttc aacgccacaa gatacaccac gtttcaagga attatttgga aacggaaatg
     9241 atttaggact gcagatcgaa tatgcagtac aagaaagtcc agatggattg gcacaagcgt
     9301 ttatcattgg agaagaattt attggagatg atagtgtctg cttggtatta ggggacaata
     9361 tttactatgg tggcggtctg tctaaaatgt tgcaacgagc agcttcaaaa gatacaggcg
     9421 cgacagtatt tggctatcat gtcaatgatc cagaacgctt tggtgtagta gaatttgatg
     9481 agaatatgca agctttgtcg attgaagaaa agcctgctca gccaaaatca aattacgctg
     9541 taactggcct ttatttttat gacaatgagg taatttctat cgctaaaggt atcaaacctt
     9601 cagaacgtgg agaactagag attacggatg tcaataaagc atatttggat aaaggaaaac
     9661 tatccgtaga acttatggga cgcggatttg cttggcttga tacaggaaca catgaatcat
     9721 tgcttgaagc atctaccttt atcgaaacaa ttgaaaaacg tcagaactta aaagtggctt
     9781 gtttggaaga aattgcttac cgtatgggct acatcgataa ggaacaattg ctggcccttg
     9841 cacagccatt gaaaaaaaat caatacggtc aatatttatt gaatttggcc gctgaataag
     9901 gagtccttac agatgaaagt aacagataca aaattaacag atgtcaaaat cgtagaaatg
     9961 gacgtctttg gcgatcatcg cggtttcttc acagaaagtt attcaaaagc aaaatttgca
    10021 gaacatggat tgaaatatga ctttgttcaa gacaaccatt cactatcagc tgaagctggt
    10081 gtccttcggg ggcttcattt ccaaaaagga gaagctgcac agacaaaatt gattcgggta
    10141 gtcactggag cagttttaga tgtagtagtg gatatccgca aaggtagccc gacttatggt
    10201 caatgggacg gatttatctt atctgaacat aatcatcgac agttgcttgt acctaaagga
    10261 tatgcccatg gattcgttac tttgacacca aatgtgaact tcctttataa gtgtgataat
    10321 tactataatg cagaagcaga tgggggcatc gcatttgatg atccagattt agcgattgat
    10381 tggccaattg atccatcaag agcgattatg tcggaaaaag atcaccgtca tccgacatta
    10441 aaagaatttg aagcagaaaa tccatttgtt tacggagaaa tctaagaatt aaggagaaat
    10501 tttcttatga aaaacatcat tgttaccgga ggtgccggat tcatcggctc taatttcgtt
    10561 cattacgtgg taaacaatca tccagaagtg catgtcactg ttttggataa actgacttat
    10621 gcaggaaaca aagagaactt ggccggactt cctagcgatc gcgtagaatt ggtcgtagga
    10681 gatgttgcag atgcagaatt agtggaccgt ctagtaaaag aagcggacgc agttgttcat
    10741 tacgcggcag aatcccataa cgataattca ttaaaagatc ctttcccatt tgtacaaacg
    10801 aatatcattg gtacgtatac acttttagaa gcttgccgca agtatgatgt acgctatcat
    10861 cacgtatcta ctgacgaggt ttacggcgat cttccattac gtgaagacct gccaggacat
    10921 ggggaaggcg aaggagaaaa attcactgcg gaaacaccat ataatccttc aagtccatat
    10981 tcttctacaa aagctggttc agatctgtta gtaaaagcat gggtgcgttc ttttggctta
    11041 cgtgcaacca tttcaaactg ttcaaacaac tatggtcctt atcagcatat tgaaaaattt
    11101 attcctcgcc aagtaacgag tatcttaagc ggtattcgtc caaaattata tggcgaagga
    11161 aagaatgttc gtgactggat ccatacgaat gatcattctt cagcagtgtg gctgattttg
    11221 acgaaaggca gaattggcga aacctatctg atcggtgcag acggtgaaga agataataag
    11281 accgtgatgg aattgatctt agaaatgatg ggccaaccaa aagacgcata cgaccatgta
    11341 aacgaccgcg cagggcacga tttgcgttat gcaatcgatt cgacaaaatt gcgagaagag
    11401 ctaggttggc agcctgagtt tacgaacttc cgtgatgggt tggctgacac gatcaaatgg
    11461 tacgaagaac atgaagactg gtggaaaaaa gaaaaagagg ccgttgaagc agcatatgcc
    11521 aaaaatggac aataatacag aggaaatttc actgtattaa tggaaaaaca catgtgaggc
    11581 aggcgctcgc ctgtctcttt tttaaaataa aaatcaagga gcggataacc atatgttttt
    11641 attaactgga ggaaatggac aattaggtac agaattgcgc catttattag atgaaaaaaa
    11701 tctgccatat gtttcaacag atgcaaaaga cttggatatc acagatgctg agaaaacaat
    11761 ggcttatatt acagaattaa aacccgatgt tattttccat tgcgcagctt acacagcagt
    11821 agataaagca gaagaagaag caaaagaatt agatgagaaa atcaatgtcg atggcacaag
    11881 aaatgtagca cttgcagcaa aagctgtgaa tgcacaattc gtctatatca gtacagacta
    11941 tgttttcgat ggaaaaaaca aaaatgtaga atacgaagta gacgaccaaa cgaatccact
    12001 aaatgaatat ggtcgtacga aattattagg cgaaaaagca gtagaagaga ttttagaaga
    12061 ctattatatc atacgaactt cttgggtttt cgggatctat ggacacaatt tcgtcttcac
    12121 gatgcaacgc ctagcggaaa cccgcgacaa actgactgta gtaaatgacc agtttgggcg
    12181 accaacttgg acacgtactt tggcagaatt tatggtatat gtcatcgaag aaaaagcacc
    12241 atttggtatt tatcatttgt cgaacgaaaa tagctgttct tggtatgagt tcgcaaaaga
    12301 aatattgaaa gatacagatg tagaagttgc acctgttacg tcagaagaat ttccgcaaaa
    12361 agcaacacgt ccgcagtatt cagtcatgag cctgaaaaag acagaagcgt taggttttgt
    12421 cataccaact tggcaagaag cacttgcgca aatgctggaa gctgccaaaa aaatgaataa
    12481 gtaatttcct ttttatcaaa tagaatttgg agcatgatat ggaaaaacaa acaagaagtg
    12541 cacgacgaaa agaaaagcca aaagcgaatc aggcagattc gaatttggtg cttgacctgt
    12601 ttctaaaact ccgctatctt attggcgttt tagtcatcgt ggtcatcgtt acttttaatt
    12661 taaacggaag ttcacttggt gtttgggata aatatgtgtc gcaaagagac gatgggaaaa
    12721 aaactgatgt gatttttgga cagaatcggg aagtgcggtc agacgaatgg ctggtgcaga
    12781 caccttttta cctctcacaa gctgaagagg acttttcgtt ggtgaacgag gattattcat
    12841 taaacggaca aaacatgatc attgcgtata attctcctgt aaaggatatt acggttatcg
    12901 gaaaaccatt caactgggga ttctttttcc tcggaagaga tcgaggactt tccttttatt
    12961 gggcgatgaa attagtcgtc atggtcttgc ttggattcga agtactgatg atattaacga
    13021 agaggaaaaa agctctatcg ctaatcggtg catttgcgct gacattttct ccagcagtcc
    13081 agtggtggtt catgcaacat gtgggcgatt tgattttctt tactttggga ctgatgattg
    13141 ctttttatca ttatttctac aatcatgata aaaaatggct acgaacgttg atgatgcttt
    13201 tggtggttat tttcggtatt gggtttattt tagtgattta cccagcccat caagtgatgc
    13261 tggcgtacct gctgattttt tattttgttg gcctgcttat ttactatagc aagaaagttt
    13321 catgggatct gttggatgta gtgttgattg gaggggcagt tttatttatt ggcggagtca
    13381 tggttcattt ctggctaacg tcaaaagacg cattgttagc ttctttgaat accttatatc
    13441 caggaaaacg ggtaagtact ggaggaaagt ggacgattgg cgaatttttt tctttcctta
    13501 caaattggaa aattcctttt gaagatatca actattcgaa taactctgaa gtggccctgt
    13561 tctatcactt ttttccaact gtattcctag caagtccatt tgtgttattt ggcaaaaaag
    13621 atagcgaaca aaagttactg ggacgtattt tgatgatttt ttgtctgttt tctatctttt
    13681 ggattaccgt tgggttgcca aaatggctag cagaaatcac tctgctgtct tatgttcctc
    13741 cattaagagc catccttact tttagttttg cggcttgttt attatcgatc tggtttatcg
    13801 cttatatttg gagagaagat gttattccta tttggtataa attcattttg ttggcagtaa
    13861 atgcatggtc ttatttctat gcgattacgc attcgaaaat ggataattat ttttcggatt
    13921 ttgaaaccat cgcagttgct gtgttgggaa cagtgatttt agcttttgca ttatttggct
    13981 ggagacgctt gttttacctc gcttttgtcg ccttagttat cgtatctggc tttttcgtca
    14041 atccagtcgt agaaggtaca ggagcgattt ttgaaaaaac actggcaaaa gaaatccaaa
    14101 aaatcgatca acaagatcct ggtaaagttt ggttgtctga agatgaatta tataacttca
    14161 ccccaacatt gggagtgaaa tccttcaata cagtacgctt ttatccagat atagctgcat
    14221 ggaagaaaat cgatccgaaa gaagaatacg agaagtatta taatcggtat gctcatacaa
    14281 gagcttttat cgctaaggaa gatacaaagt tcattttaga tcgtccagat atgttttcgg
    14341 tcacgttgaa ttttgaagac gcagaaaaat tagggatcga ctatgtgatc agtaaacgac
    14401 cattaactga ttacaaccaa ttatatgaag cgcagttcac gcagctatat ggaccggata
    14461 aagacggtta tatgattttt aaagtagaat atccggatta tccagatgta ccacaaacac
    14521 aaactcagga aaatcagcta gtcgtacagt aataggctag ctgagaataa tcctgaaata
    14581 ttagtagaaa ggagagtgaa gataattcaa gcaatcgtac aaaaatttgt caacaaaatg
    14641 agtgtcggtt tcttgctttt gttgctgacg ctagtctttt ttgtttggac agcaggggta
    14701 ggttttccag caactttgtt tatatataac aatagttcat tacttatcgc ttttagtgtg
    14761 gctattttgc tcattttaaa tgtttataaa gcaaaaggcg tcgattttct ttatctaggt
    14821 gttgcgcttg cccttttcat tttcttttct tactttcggg aattaagaaa tgagtcttta
    14881 tttggagact tactgtttcc tttgattatt ttattttgtt tagtaattaa atggatcgag
    14941 tggacaaaat gggatcgcgg tatttatctg attgtcttta cggtcttcct cggaatcacg
    15001 ttatatcgtg tatttacaga gatccaagta ccagaaggac aaagcatctg ggctccaggc
    15061 aattcgcttt ctgatatttg gatcaatacg aatacaatcg gaagttcttt gatgactctt
    15121 gctttattga tcagtggatt tgccagttct tttgaacgat ggtatgttcg aatcatcggt
    15181 gggatagcag tcgttgcagc ctttttaggt atttgggtct gccaatctag aggtgcatta
    15241 gtcgcgttga ttgtttttgc gatattggat cttttaccta aatcgttgat gcgtgtcatt
    15301 cgtgcgccat ttattgccta cacggttacg attcttttag ctttgccgat ttcttatctg
    15361 gcggctgtct ctgaaaaagt caatttgttt accggtcgag aagatatttg gcataaattc
    15421 tatcagacgc taggagaaaa atctgaacaa gttttactag gaatgaaaac atttatcttc
    15481 cagcgaggaa accaattttt aggaaaccac aatagttata attctatttt gaatatctat
    15541 ggattgatag gatttgggat tgctgctttg ctgcttatcc tttttattgg tcgcttaacg
    15601 ttgaaagcag atttatccaa cgggcagatg acttttatct gggccttttt tgcggtgatg
    15661 atgcagtcat ttatggagga tacattgact tcataccgat gggttccgat tatttatatc
    15721 ctgttggcta tggccgttca tcgatatgat gaaccagaag ttacggagga attctatgtt
    15781 atggaagagg catatgacga tatgacttat gatgatacat cttatgagga acaaccagat
    15841 agcgctcatt cttccaggat ccaacgctat cattgatagg cacaaaatac gagagaggct
    15901 gtgacaaccg tcgcagcctt ttttggttta ttgcaattgt ctagcatctt ctgtaaaata
    15961 gaaacgttag agaaaatctt gtatcataga aaattaagaa agaaataaga acgcagctcg
    16021 gtaccaaaaa tatggtaaaa tggcacttga ctgtaattca ctgaaaatcg cttggaggaa
    16081 cacaaatgtt taaagatttg tatttattta tgaaggacat ttatcagaac agaaaattac
    16141 tgctacagtt ttcgatcaat gacttcaaat caagatatgc aggatcgttt ttaggaatct
    16201 tatgggcgtt tatcaatccg ctatttactg tattgattta ttggctggta tttggtgttg
    16261 gactgaaagc acaaatgatc gatgcaaaat acccatttat cgtatattta gttaccggga
    16321 tgattccctg gttcttcttc tcagatgcat ttgcatcaac tacggttgtt tttcgggaat
    16381 atacttattt agtaaagaaa gtcgtattca atattcgtat cttgcccacg acgaaaatcc
    16441 tatccaatct gtacacccat ttattcttca ttctgattgg catagctgtc gcgcttataa
    16501 atggtatata tccttcagca atgtctttac agctgattta ttatttattt tgcatgtgtg
    16561 ctttcttgac aggattgact tggctgacgg catcgatcca accattttta cctgatatca
    16621 tgcaattaat tacgatcgtg ctgcagttga tcatgtggac attaccgata ttatggagcc
    16681 ctaatcagtt tgattcaggt cttgtaagca tattgaaatt aaatccgctg tactatgttg
    16741 ttcaaggtta tcgtgagtca tttttaagtg aagcttggtt ctgggagcat ggaacatata
    16801 ctttgtattt ctgggttttc acgttagtaa tgttcgtaat cggctctgca gtgttccgta
    16861 gattgaaacc acatttttca gatgtgctgt aaggagaata gctatgacag aatatgcaat
    16921 tagactaaaa gatatcacaa aatcttataa tatgtacgca aaaccttcgg atcgatttcg
    16981 tgaagccctc aatcctttta agaaatccta tcatgatgta ttttatgcgt tgaaaaacgt
    17041 gaatgtagaa ataaaaaaag gtgagatgat cggtttcgtg ggagaaaacg gatcgggaaa
    17101 atctacaatg ttgaaaatca ttacgggagt actgacacca acatccggca cgatggaaat
    17161 cgaaggaaaa atctcagctt tgctagaact aggatcagga ttcaatcctg agtattctgg
    17221 ctatgaaaat atctatttaa atggaatggt tctaggattt tcaagagaag aagtcgatga
    17281 aatggtcgat gatatcattg aatttgctga catcggagac catatttatc aacctgtaaa
    17341 aacttattca agcgggatgt tcgttcgtct agcttttgct gtagcaatca atgtgaatcc
    17401 cgatattttg attgtggatg aagcattggc agtaggcgat cttgaatttc aactaaaatg
    17461 tatggaaaaa tttacagaaa tcaagaactc gggaaagacg attttattcg tttctcatga
    17521 tatcagttcg attcgtcgtt tttgtgatcg ttcctattgg ttgaaaaacg gagaagtcgt
    17581 cgaatatggc gatacgatgg aagtaacgac gaattatgaa aactttttaa agaaaaaatc
    17641 agttaaaaca gtggatcgga ataaaaacca agtagaacgg ttcgccgctc cagatatcgt
    17701 cgacgtgacg aaagcggaat tgttggataa agacaaaaat ccggtggata tgctggaaca
    17761 aggagaaaaa ctatacgtta aagtaactta tgaagtaaaa gatgatacaa tcaaaaatcc
    17821 ggttttagga attgcgctac gaacagtcga caatttttat gtatgcggac ttaatactct
    17881 actagatggt atcaagattc cttggaaaaa aggagagaat atcttttatc tggagtatga
    17941 aaagttaggt ttgttagctg gtgaatatta ttttgacgtt gcggtctttg aagaaaatgc
    18001 aacggttcct ttggtatata agacaaagta catgaacttg tttgtcagcg gttcttatat
    18061 cggagaaggc atcgtggtct tagaccataa atgggaagaa gggactcata gcaatgaaat
    18121 atgattttga aatggaccta gatgaacaaa gcagtgtggg aaaaattgcc gcacagatca
    18181 aaccaggaag taaagtttta gagtttggtc ccggaaacgg ccgattaacg aaacatttaa
    18241 tcggagcaaa acaatgtgaa gtgagcattg ttgagttaga taaggaattg ttcgattttg
    18301 tcagcgaatt tgctcaagat ggattttatg gtgatatcga aagttttgag tgggcaaact
    18361 attatgctgg acagacattt gattacgtct tgtttgcgga tgttttagaa catttggtag
    18421 atcctggaaa aacattgaag aaagtcagag aatttttgaa tgaagaagga gagatcctga
    18481 ttacattccc taacttggcg cataattctg tcatgatcga tttgttcaat aaccaattgc
    18541 cgtgggcttc ttatggtctc ctagatgaaa cccataattc tttttatact catgatggct
    18601 tccaaaaagt ttttgaaaaa gcgggattat tcattaatat tgaagattat ttgtacttag
    18661 ctgtaggtga tacagaatta aaatcaactt atgaagaatt gccggaagct gtacgctatg
    18721 atttcaaaat gcgaccattt ggtgaagtct accaatactt tttctcattg atgaaacatc
    18781 cagtagcaca aagttccatt gcagagccac aaaattctaa ttatgtaaaa gtgttagaag
    18841 taacccagca aacgaaacaa gatgaaacaa tccaacaaat accattcaat aatttcacag
    18901 gggaaaatga gacactaagt ttccccgtat cagaaacaac agaaagaatg gtgtttcgct
    18961 tcgcgcaaca acctagcttt atcgaattca gcgcagaagc tgccggagaa aaactaacat
    19021 ttatcgattc gaatgctgta gtcaaaacag taaatgattg ttatttgttt gatgggaaag
    19081 aattaccaga gttcgttcta acggatattt ctgggaaaga agtgactatc cattgtcact
    19141 atcgctttat cggggaattg acaccaacaa tgaaagaatt gttggaaacg attcgtccca
    19201 tggctgaagt cacaaagcag ctttctgaga aaaatgacga attagaaaga gaaaatcatc
    19261 atcttcttga ggaaaataca agactagacc atacgttaaa aacaaccaca aatcgctatt
    19321 gcacattgtt ggaaagtgat gaatggacaa taaaacatag actaggacga cgtaagaaag
    19381 aaacttcgaa aaaaatccaa gaaaaagaac tttctatatg tattgacgag aaaatctggg
    19441 atgcagaaac aaaaatatta aaaatcatcg gatggggaat cgctaattcg gatcgccagc
    19501 ccttgtccta taagttatca gctgaccaat cgccattttt cgaagcaatt cctgtttcac
    19561 gagaagaagt caatcaagca gaacagctcg caaaaggaac agaagcggga tttgagttac
    19621 gaatcctttg cgagcaagaa cgttcattct tagtggaagc agtagctcaa aatggccaat
    19681 catggatcat tgaaatgtaa ataatgatta acttataaag ataaggagta aacgaatcgt
    19741 gcaaaaaaaa gaaattgccg tatttatcga tcaaatcact cgtgaacggg ccactggcga
    19801 tttgcttatt gtgggctggg caattgatga agtgaccaaa gaaatcccta cgataaaggt
    19861 agaaaaagaa aatgtgattg ctgaagcaac acatgtcgta agattggata tcaatcattt
    19921 atataatctt gatgttaaga cccaaagcgg ttttaaggta cgtttatcag ggaaaatgag
    19981 aggaaaagcc atcttagact tccaaacagc gagacatcaa aatggcatag ctgtgaaatt
    20041 aaacggtaaa tacccttatg atgacggcat tgaatcttca tgggaaagaa aaaaaagatt
    20101 actgaaaaaa gggataaatt atgcaaggac tcatggcgta aaaaaagcga tccgtcgggt
    20161 caaattggaa ttgaagccag gatcgatcga ctatgcagag tggatcagtc gtcatgagaa
    20221 accagatttg aaagagcaaa ggaaagaagc acaaggattt gcgtaccgtc cattgatttc
    20281 catacttatg cctgtctata acgttgaaat caaatggctc gaaaaatgca ttgattcggt
    20341 actggatcaa acgtatgatc attgggaact atgtatcagt gatgatgctt ctactgatcc
    20401 agctatccga aagtgtctgg aatcctatca agcaaaagat gatagaatca aagtcgtttt
    20461 ccgaaaagaa aatggacaca tcagtctggc aactaattct gcattggaaa tagcagaagg
    20521 agaatttata gcactcttag ataatgatga tgaattaccg ccatttgcat tatatgaagt
    20581 ggcaaaagtg ctaaacgtac atccagaact ggatgtgatc tacagtgacg aagataaaat
    20641 cgatgcagat ggcaatcgct ttgatccgca tttcaaagca gactggtcgc ctgatacatt
    20701 gatgggaaat aattatatct ctcacttagg cgtatatcga acaagcattg ttaaagaact
    20761 tggcggtttc cgtaaaggct atgaaggttc tcaagattat gatttggtcc ttcgtgtgac
    20821 agaacaaatc cctgcagatc atatttacca cattgaccga gtcttgtatc attggcggac
    20881 gatcccagga tcaactgcaa gtaatggcga agccaaaagt tatatctatg attccggagt
    20941 gaaagcattg acagatgccc tgtctagaag aaacatcaaa ggaagcgtac gtcctggcag
    21001 aatttctgga ttttatgaga ttgcttatga cgtcttgcaa gaggatttgg tcagtgtgat
    21061 cattccaacg aagaatggct atgaagatct aaaaacttgt gtggattcga tcatcgaaaa
    21121 aacgtcttat tcaaattatg aaatcattat tgcggataac ggaagtacag atccaaaaat
    21181 gcaagaatta tttgcagaat acaagcatca gctgaaagac cgctttatcg tagaattgat
    21241 tgacattcca ttcaattatt ctcgaattaa caatttagca gcagaaaaag caaacggcaa
    21301 atacttttta ttcttgaata acgatacaga agtgattgag ccagattgga tgacggtgat
    21361 ggtttcttat gcacagtttg atcgaatcgg ctgtgtagga gcaaaactgt tttatccaga
    21421 tgatacaacg cagcatgcag gagtattatt aggaatcggt ggtgttgcag ggcatgcatt
    21481 gaataactat gatcgaacac attgtggtta tttcggccga ttggtcattg acgtcaatta
    21541 cttagcagtc actgcagctt gtatgatggt caaggcagct gacttcaatg ctgttaacgg
    21601 gtttgatgaa acattagaag tagcattcaa cgatgttgac ttgtgcttga aagtttatga
    21661 actgggtcgt tacaatgtct atgcacatca agcagaattg taccatttcg aatctaaatc
    21721 aagaggttat gaagatactc cagaaaaaca aagacgtttt gctggtgaaa tcaaaaaaat
    21781 gcaagataaa tggccagcat atattgcgca tgatccattt tacaatgata atttaacaaa
    21841 agaaggaatc ggcgactttt cgttgagacc agattaaact ataataaaaa gctgttatgt
    21901 tgtgattcgc tcgcacataa cggcttttta tagttttttg tcaaaaaaag gcagaaggtg
    21961 ccgactcaaa aaaataagtc gaggttggga cagaagtgtt taactccgag aaataagaag
    22021 aaattcacga aaattgcttt tcaaattttt gtgaatttcg gcttatttaa gaagaagttg
    22081 cttctgctcc caccgtttat tcgtttttaa agaatgagac aaaagtgttt cttacttttg
    22141 tcccacgctc ttattcatgc tcagagcgcc aatagataca aaatcatagc caaagtttca
    22201 cgaaccctag ctgtcaaaga atcgatattt tattttaata taggctattt taaaataaat
    22261 ggtttaaggt attcctctac aaaagccact ctatcaatta tcatgggttt aaaataagaa
    22321 gtgatggaga taaccagatt gttcttagcg tcaatataaa gaacatttcc actattacca
    22381 attgctgaat atatttcgtt ctcagaatct aatacccacc ataaatagcc atatctcata
    22441 ttttgtaagt cttctaaagc actggttcta ggtttgaaca tctcgtttat ccacatgggt
    22501 ggtacgattt gttttccttt atacatccct ttatttaggc ataatacacc aatttttgcc
    22561 agatcacttg ccgataaaca caaaccatat cctgccgtcc cgatatttag tggatcacaa
    22621 aaccagacat tggttttagg agacttatcc attgtaaact gcttatgttc ttcagctgtt
    22681 tcagcataat aattgacatg gtttggtatc cctataggat caaatagata cttattggca
    22741 aaatcaacag ttgttagctt acttgtctga tacaacaagc cagttaatat gtgtaaacag
    22801 acagtttgat agttgaattc gccggttata ccttttcttc ctcccaataa atctagtgat
    22861 gtttttgtcc aatcttcact tgaacagact tttgtccatg gatcgccttt acatttgtag
    22921 ggtgctttca ttgttagcaa gtgcttgatt gtaatagcgt aaatagtttt ctccccgcgt
    22981 ttcactttat actctggaaa ataatctaat actttatcat cgatactttt gattaatcct
    23041 tgctctaaag caatgccaat cagcaaagat acaatacttt tcgttgctga catgatatga
    23101 caagtatcgt cttttttata accattccat tcatctgtat aaacttctac cccatcctta
    23161 tatgcgacaa tttgacatat atttggttgt cgaaaaccaa taaaatcatg caactcttgt
    23221 ttattcataa gatattacct cactttcttc tataagcata atgtcattaa aaatcgattt
    23281 caattttttc gattcttcac gtaatacagg tattcttatg gactagacag cgctttggga
    23341 agatggattc tctgaaaaat ataaaggttc actttatgag tggataaaaa aagcagcaaa
    23401 tacttcgact tctttaattt accaacatta gtgaaaatcg attcatctgg agatttgtaa
    23461 tttagagtcc ttgtcttgat tagaggtgta taaagctttt tattagacat ttttaaattc
    23521 cattacaatt tgcttataaa ctaaaggtga ctatggtaag ataatagaga tattattttg
    23581 tgattaggag tgtatatatg aataaaaatg gagaatggag tgctgcacga cggatattca
    23641 ttatattaat ggatgttatt gtatacaatg cagcaatata tagtagtttc ttactgaagt
    23701 ttcgtggaga gatcccatta cgaaatttag aaactttcca acactcagca attattattt
    23761 cagtgatttt tattgcgttg aacattttgt tgggtgcata tgttttttat aatcgaatga
    23821 tcagtgacat cgtgtttgtc acagtcatcg gtcaggtatt gatgacacta gggatcatgg
    23881 tagtgacttt tgcgggccgc tggtttgctt ttccacgtct tgttatctta ctttcatttg
    23941 ttctcggtac gcttttctta agtatctatc gtattctcgt atataaactt tatttacgcg
    24001 taagtcgaga taaaaaggtc gtcattattg ggttagaaaa ggatgtcgca ccagcaatcc
    24061 aaaattttgg ttcaaagaaa aacaacaaac ataaagtcga agcagtcatt cttgataact
    24121 attatgcaaa catcaaaaat atgattaaag acatcgatat tgtctatctt gcttctcata
    24181 tcgaagaaac tgaaaaaatc aaaatctatc aattagttac taaaaatgaa aaaaaattgt
    24241 ttttgaatac aacatttgaa aacttgacat tgatcaatcc aaatattatg aatttcgaag
    24301 atgaaagtat tattgaaaca tctggattta gaatccaacc tgaatatgaa ctattcaaac
    24361 gtttgtttga tatcatcgtt tcacttgttt tattagttat tgcttcacca tttatgttat
    24421 tcacagcaat tttagtcaaa gtgacatcac cgggaccaat catctataaa caagtgcgga
    24481 tcacaaaaaa tcaaaaggaa ttttcaatct ataaattccg ctcgatgacc gccacagccg
    24541 aagcaaaatc tggaccagtt ttagctaaga gcaatgatgc acgagtaacg cctgtaggta
    24601 aatttattcg agctgtccgc tttgatgaat taccacaaat cttcaatgtg ctgaaaggcg
    24661 atatgtcgat cgtaggacca cgtcctgaac gtcctttctt cgtcgatcag ttcaatgcag
    24721 aaaaccctta ttactactta cggcataatg ttcgtgctgg tatcactggg tatgcacaag
    24781 tctatggaaa atatgtctct gattacaaca gtaaattacg ttttgattta ctttatatca
    24841 aaaaatattc attgatgctt gatttaaaga tcttgctgca aaccatcaag atcctatttg
    24901 ataaaatgtc ctctcaagga cttgaagaag acgaaaatgt gatctcattg gcagaaattg
    24961 cttttccgaa tgaaaaaatc tttcgctagt tgggaggcgt aaaaatgaac aatccgaaat
    25021 tcagcataat cgtaccagta tacaatgcag aagcaactat tggtcgaacg attgatattc
    25081 taaaagaatt ggattatgaa gagtacgaag tcgttttagt aaatgatgga tcaacagatc
    25141 aaacacagga aatcattcaa gaactgattg aaaatgatga acgttttgaa ttgattacta
    25201 cgaccaatca agggcccgga ttagcaagaa acgaaggaat aagacagtca aaaggagaat
    25261 atctcttatt cttcgatgca gatgacacgc cagtaaaaac gatcttgtca gattatgatc
    25321 atttgctaga aaagagtcca aaagcggatc ttgttgttag ttcgtttgtt tttcgtacaa
    25381 tggacgaggg aagaatcgtt tccgaaaaag aaaacttagt tacagaatgt cactatctaa
    25441 ctcacgaaga gtttataaaa gacatgtatc agttaatgaa tgaacagttg atgtatgttg
    25501 tatggaacaa atgctatcgt agcgatattc tgaaaaataa ccaaattcta tttaaaactt
    25561 ataatagttg tgaggatcga atatttaact tacattatta tgaatactgt catcaagtga
    25621 tcatgaaccc aaaagtagaa tatatttatg aatttgaagg tgggaagggg atcactaatc
    25681 aataccgacc taacaagttc gaaacattta aagaattcta ttcattagca aatactgtaa
    25741 cagacgatat taataaagac ggtatggcgg cgcttctttt aaaagggaca acttcagtta
    25801 ttttttctat ttatagtacc tctgcaagaa cttcaaaaga gaagaaagac gaagcaaagc
    25861 agatattgag tgatccagtg atccgagaag ccaagcaaat tgctaagacg gattcttcag
    25921 caaaaaaaat aacaaagttg ctttacaata tgccagctcc actattttta tctgctgtaa
    25981 aagttggaag cttcgttgaa gtgaaaatgc cagggttaat ggctgctttg aagcgagtat
    26041 attagttaat ggaggaaaat ataatgaaaa gaattttgca ttttcaggga cgaatgggtt
    26101 taggtggagc agagtctttc atgatgaatt tatatcgtaa aattgatcgt acaaactatc
    26161 aatttgattt tttgatttat gaagattatg cagatgttca agactatcat tcagagattg
    26221 aaagattagg aggccgtatt tttgttgtac ctaatcctaa gaaaaatatt tttaaatatt
    26281 taatagaagt gaataagtta ctaaaaaaag aatctttttc tatagttcac aatcaggttt
    26341 attttggcgg gggaattaat ttatggttag ccaaaaagaa tggaatcaga cagagaattg
    26401 ctcatagcca tgcaactgaa gatggaaaat ctcaaaatat tgtaatgaat gtattgcgta
    26461 aatttttaac caaattgttg ttacaaaatg caacagacta ccttgccgtt agtcaagaag
    26521 ctggtgaaag tctgtttcaa aaccatccct ttgagatagt tcataatggt attgatttag
    26581 agctttatag taaaaattca gaagctaagg ttaataagag aaaagaattg gatatctcaa
    26641 tgagtacttt tgttgtggga aacattggac gattagagaa acaaaaaaat caatcttatt
    26701 tgttagagat ttttttagaa atactaaaaa tgaattctga ttcttacttg attttagtag
    26761 gtgaagggtc acttagaaat aaactgaaag ccaaagcagt agaattggat atagagaaaa
    26821 gagtaaaatt tttaggagaa agaaacgata ttcccgagtt attatctgtg atggatgttt
    26881 ttgtaatgcc ttctctttat gaaggtctgc caatggttgg attagaagca caagcagcaa
    26941 aattaaaatt agtcttagca gatactattt ccaaagatac aaagttaaca aataatgtgg
    27001 attttgtatc attagatgaa acaccacaag aatgggcaaa aaaagtaata aaagagccgt
    27061 atgctaataa aataacacaa gaactaaaga gttatgatgt ttcgtacact actgaacaaa
    27121 tggagagtat atatagtaga gatcattaaa aatgttcaag atattttagg agtgattatt
    27181 tttgaaaata tgttatttga tattggcata caatgatatt ggcaacttaa agagattggt
    27241 caatcgattg aacgaaaatg cagtttttat tattcatata gataaaaaaa ctgatataga
    27301 accatttatt gaagcattta aatcatatga taatgttgaa tttttatgta ataaaaaccg
    27361 tgttaaaatt tattggggtg gtttttccat tgtacaagca gaaatcaatt tagtaaagag
    27421 agcattacaa aatgagaaat atttaaaata tgtattatta tctggtgctg attatccaat
    27481 caaagataat gagtatattt ataattattt taaaaagaat
//
LOCUS       DAZKKW010000032        27255 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.032, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000032 DAZKKW010000000
VERSION     DAZKKW010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27255
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.032"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(38..634)
                     /locus_tag="ACG8PO_001312"
     CDS             complement(38..634)
                     /locus_tag="ACG8PO_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFQ0206651.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
     gene            984..1328
                     /locus_tag="ACG8PO_001313"
     CDS             984..1328
                     /locus_tag="ACG8PO_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFQ0206652.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVEWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            1329..1748
                     /locus_tag="ACG8PO_001314"
     CDS             1329..1748
                     /locus_tag="ACG8PO_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HFQ0206653.1"
                     /translation="MMTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVTFEPGCRNHWHVHTDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(1840..2190)
                     /locus_tag="ACG8PO_001315"
     CDS             complement(1840..2190)
                     /locus_tag="ACG8PO_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0206654.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(2156..3487)
                     /locus_tag="ACG8PO_001316"
     CDS             complement(2156..3487)
                     /locus_tag="ACG8PO_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HFQ0206655.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGVFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSVLSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGILLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLSGIREIFDSSGKLTT
                     DFIANSGIGATLINMGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVHN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
     gene            3742..4308
                     /locus_tag="ACG8PO_001317"
     CDS             3742..4308
                     /locus_tag="ACG8PO_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0206656.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            4453..4540
                     /locus_tag="ACG8PO_001318"
     tRNA            4453..4540
                     /locus_tag="ACG8PO_001318"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4485..4487,aa:Ser,seq:cga)
     gene            4876..5850
                     /gene="bsh"
                     /locus_tag="ACG8PO_001319"
     CDS             4876..5850
                     /gene="bsh"
                     /locus_tag="ACG8PO_001319"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HFQ0206657.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNGTPENHFSNQISLNVYSRGMGGLGLPGDL
                     SSVSRFVKATFTKMNAASGDSESESISQFFHILGSVEQQKGVCDTGEGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAVNINKEDLDRHELVSYPVIKEQQINYVN"
     gene            6175..8070
                     /gene="asnB"
                     /locus_tag="ACG8PO_001320"
     CDS             6175..8070
                     /gene="asnB"
                     /locus_tag="ACG8PO_001320"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HFQ0206658.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKLDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
     gene            complement(8265..8711)
                     /locus_tag="ACG8PO_001321"
     CDS             complement(8265..8711)
                     /locus_tag="ACG8PO_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HFQ0206659.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            8939..9088
                     /gene="rpmG"
                     /locus_tag="ACG8PO_001322"
     CDS             8939..9088
                     /gene="rpmG"
                     /locus_tag="ACG8PO_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFQ0206660.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            9176..9694
                     /locus_tag="ACG8PO_001323"
     CDS             9176..9694
                     /locus_tag="ACG8PO_001323"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFQ0206661.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(9771..10727)
                     /locus_tag="ACG8PO_001324"
     CDS             complement(9771..10727)
                     /locus_tag="ACG8PO_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0206662.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            10893..11699
                     /locus_tag="ACG8PO_001325"
     CDS             10893..11699
                     /locus_tag="ACG8PO_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0206663.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKITLLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            11696..12685
                     /locus_tag="ACG8PO_001326"
     CDS             11696..12685
                     /locus_tag="ACG8PO_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFQ0206664.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVGKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            12682..13686
                     /locus_tag="ACG8PO_001327"
     CDS             12682..13686
                     /locus_tag="ACG8PO_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFQ0206665.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIGLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILPSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            13818..14360
                     /locus_tag="ACG8PO_001328"
     CDS             13818..14360
                     /locus_tag="ACG8PO_001328"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFQ0206666.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            14380..15078
                     /locus_tag="ACG8PO_001329"
     CDS             14380..15078
                     /locus_tag="ACG8PO_001329"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HFQ0206667.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            15099..15311
                     /locus_tag="ACG8PO_001330"
     CDS             15099..15311
                     /locus_tag="ACG8PO_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HFQ0206668.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            15311..16273
                     /locus_tag="ACG8PO_001331"
     CDS             15311..16273
                     /locus_tag="ACG8PO_001331"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HFQ0206669.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRYLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACANLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            16291..16686
                     /locus_tag="ACG8PO_001332"
     CDS             16291..16686
                     /locus_tag="ACG8PO_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFQ0206670.1"
                     /translation="MVLWVINIILLLIVLAIIFWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            16849..17214
                     /locus_tag="ACG8PO_001333"
     CDS             16849..17214
                     /locus_tag="ACG8PO_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HFQ0206671.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            17211..19022
                     /locus_tag="ACG8PO_001334"
     CDS             17211..19022
                     /locus_tag="ACG8PO_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HFQ0206672.1"
                     /translation="MKIGIIGAGPRGLSIAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNDLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSREGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGSMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLEIPLTYQDESFAEASRAFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPVIRTKELAALIDAGIFHLFEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(19084..19251)
                     /locus_tag="ACG8PO_001335"
     CDS             complement(19084..19251)
                     /locus_tag="ACG8PO_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HFQ0206673.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            19500..20489
                     /gene="galE"
                     /locus_tag="ACG8PO_001336"
     CDS             19500..20489
                     /gene="galE"
                     /locus_tag="ACG8PO_001336"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFQ0206674.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREVLGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            20501..21985
                     /gene="galT"
                     /locus_tag="ACG8PO_001337"
     CDS             20501..21985
                     /gene="galT"
                     /locus_tag="ACG8PO_001337"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0017103 - UTP:galactose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019388 - galactose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HFQ0206675.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLADYPQVIAGVVKWPMSVIRLQSTEKKLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            22009..22989
                     /locus_tag="ACG8PO_001338"
     CDS             22009..22989
                     /locus_tag="ACG8PO_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0206676.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPTDGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            23180..23902
                     /gene="tsaB"
                     /locus_tag="ACG8PO_001339"
     CDS             23180..23902
                     /gene="tsaB"
                     /locus_tag="ACG8PO_001339"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFQ0206677.1"
                     /translation="MITLGIDTANQTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAID
                     QLFADLQISPKDIDRIAVSDGPGSYTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAAN
                     CIGVDKIIVPMFDARRKNVYAGAYRWKENRLEIVLEDVHISASDLFTQLQKLNEAIYF
                     VGSDCHKFFYEIKEILPSAMINTVPAWDIPSGVTVAQLGALAEPVAEKQAFLPRYLKR
                     VEAEEKWLETHTPGDESYVEKI"
     gene            23886..24428
                     /gene="rimI"
                     /locus_tag="ACG8PO_001340"
     CDS             23886..24428
                     /gene="rimI"
                     /locus_tag="ACG8PO_001340"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFQ0206678.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTIEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            24435..24899
                     /gene="rimI"
                     /locus_tag="ACG8PO_001341"
     CDS             24435..24899
                     /gene="rimI"
                     /locus_tag="ACG8PO_001341"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFQ0206679.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLVEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            24896..25912
                     /gene="tsaD"
                     /locus_tag="ACG8PO_001342"
     CDS             24896..25912
                     /gene="tsaD"
                     /locus_tag="ACG8PO_001342"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFQ0206680.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIVAGGVAANQ
                     GLREGLQDALATKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(26484..27185)
                     /locus_tag="ACG8PO_001343"
     CDS             complement(26484..27185)
                     /locus_tag="ACG8PO_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFQ0206681.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
BASE COUNT         8850 a         4687 c         5638 g         8080 t
ORIGIN      
        1 acattgtcaa tcaatgacta attttacaaa ataaatatta tccaagaaga ccacttagat
       61 attgacctcc ataaatcaag gccatactat ataagaaaat cgacagtggt ttagcaatca
      121 aaatgattag tgtatatcgt ttcactgaca tgtttgtcaa tccagccatc aaacataacg
      181 catcgtcagg agcaactgga agaaaaatag ctagagcaaa cagcttctca aaacggtttt
      241 gattatccag ccagccaata tatttgttat atgtcttatc gctaatcatg gaaagtatga
      301 atggctttcc ataacgacgc cctagcagaa aaatgataat cgaaccaatg cagattccaa
      361 tatagttata gataaatcca gcataagggc caaatatcag gacaccagct gcagtactga
      421 tccctccagg aatgattgga ataactactt gcaggatttg gatgaagaca aaaataatcg
      481 gtcctaaaat gatggaatca cctactaatc ctcttaacgc attcaaatct ttgaaaacac
      541 ctaaattaat aaaataaatc gtcagcgcga ttgataggcc taatccgata atggatatga
      601 aatttatcag ttttcgagag gcagcaatat tcattattca tcaactcctt agaggttctt
      661 tgttaactat actttactag aaaagcaact aactgcgcca ttttttgata tgacagaaaa
      721 caaacaattt tgtaagttca tcataagtat aaaagattga tctcaaaaaa acatccaact
      781 aaagacttat ttaataactt gtttttttta aaataaaaaa agcgttagca actatgctta
      841 aaaagcatat cgactgaata aactaagcat tggacaggct gaaagactac atttaaaatg
      901 aaatgcgaag aaacaataga aaataaagtt gccttagaac catagttgaa tcacctaaca
      961 gtcgtgaagg gagaataaga gaaatggcag aaaaacaaac cgcaggacga gatcaattag
     1021 gagattttgc accaaaattt gcagaattaa atgatgatgt tttatttgga caagtatggt
     1081 ccagagaaga attatctgct catcagcgta gtatcatcac catatctgca ttaatttcag
     1141 gaggaaattt tgaacaattg ccagctcatc tgaagattgg caagcaaaat ggcattacta
     1201 gtgaagaaat agcagaaatc attacccatt tagcttttta tgtagaatgg ccaaaagctt
     1261 ggtcagcttt tagtttagca aaagaaatct atttagaaga aaatagcgaa gataagaaag
     1321 aggcgtaaat gatgacaaaa cacgaagaaa tacacaatgg cgtgattttt ccagcaggtg
     1381 aaaaaaacga agcttacgct caagtattta ccgggcaaag ctatctgaat atgttagtga
     1441 atgatcccga agtttctgtt ggtgtaggta atgtaacttt tgaaccaggt tgtcgcaatc
     1501 actggcatgt ccatacagac ggctatcaaa tcttacttgt gaccggagga gaaggctggt
     1561 atcaggaaga aggaaagcca gcaagacgat taatggctgg agatgtcgtc gtaacgcaca
     1621 aagggattaa acattggcat ggtgcgacaa aagacagttg gtttgaacat ttggcgatca
     1681 ctgctggtaa ttcagagttt ttagaacctg taacagatga agtgtatgaa ggattggaaa
     1741 atcaataatg tcaagcttga acagctcctg aaagtagatt ttaatcgaac tttcaggagc
     1801 tgcttttttg attcagtctt ttataaatag aaaatagggt taataatagg aatgagaagc
     1861 acgtcgaaag atcttaatat tgataatcgg ctgggcatac atgatttcca cgtcatcaat
     1921 caaactacct aacagatgat aatcttcccg atcatgtcgg ggattaccta atagttcttg
     1981 atagtagatt tccatactag ggtcccgagg attgttcagc aaatcagcaa gtacatctaa
     2041 atcttctggc tgagtttctg ccgtaccctg tatctctatg aaaagccctt cctcagcaaa
     2101 ttctactcca atacgtattt cagttatttc atgggaatat aaataagtaa gaagctcatc
     2161 gatgattctt tttgataatt ctttttccaa gtgtcttttt ttcctttctg acagcggtaa
     2221 agttatctag tatcggtaca aggatacctg caacgaaacc agttgaaaaa ccactgttat
     2281 atagatttag tcctccgtgc aaagtgctga cgttatggac aagagcaata tgtagcacac
     2341 cagctattac accgtaaaaa ctgccgtaaa aaccgcttat gggcgcaaga cctgtcccaa
     2401 acacagctgc aacaagcatg ccagtactgg tgatctcatg aaaagttcca aacaaagaag
     2461 caataaatat tccgactagt atcggaaaac tgttttttaa atgacaacca aaagcactga
     2521 aacccaccgc agataaaata gcaccgatga caggtccatt taactgaccg ccaaccagca
     2581 aaacataaga agacagcatc agaccaacta agcccatgtt gatcaaagta gcaccaatac
     2641 cagaattagc aatgaaatca gttgtcaatt ttccagatga atcgaaaatt tctcgaatcc
     2701 ccgaaagccg aaaggaatta taatagaaac caattccgac tgtcagcgaa aaaaagccaa
     2761 aaataatcca aatgagagga aagtgatatt cattgaaaac caatgtattt tcgacgattg
     2821 aatagctgaa taacctcaaa acagcagtaa aagtcatcgc aatcaaccca gaggtgaatc
     2881 caatattata aagtgtgaat ccacgatgga aaactaaagt ttgagcagct aagggcggaa
     2941 gaagtatacc aattgctatg ccagctaaat tccccaataa aatacctatc agtaaaggaa
     3001 aacgcatacc aaaagtgata taactaacga ccggtgataa tacgctacca aacagtccga
     3061 tcatgatgta ctgagaaaaa ggtttattga caaatacagc gtatagatac acacctaaaa
     3121 tgattgagat agaattataa aggtttttgc caaaaaaaga gaaaccggta actgttagaa
     3181 tcgaagcaaa taagggaccg tttaaaatca ccttgtgccg ataagctaaa agcacagaaa
     3241 gtaaggtcaa aatccctaca tttatgaatg cgcttccgaa aacacctaaa gcaaaataat
     3301 ctgtcaaaag attgcttggc gaggtaagaa tagttagcaa tccactccaa ttatcagtag
     3361 gagtattaga aagcaagcca ataaaaagaa aaaaaacacc tgtaaaacca agataaaaat
     3421 actttctttt ctgtgaaacg attcgttttt gaggatgaat ataaataacg tcagtttcgt
     3481 tttccattac acaccttctt tttcattttc tttaactata acttgaaatg aaagatttgg
     3541 acaactatca tttaggtcaa aactaaaaat gtaagggaac ccattgaatt taaccaaatt
     3601 tttttaattt tgcaaaaaaa agttaaagaa gactgaaaaa aatttaaaat ataacgtttt
     3661 gagtaaaatg tttgcaaaaa atgctaaaaa tagggtacaa taaaacaaca tatactgcag
     3721 taaagaagtg ggggagtttt aatgagaaga aaagtctata ccaaggatca aattctaaaa
     3781 gctgcttatg atgttgttgc aaaggaaggg tttaaaggat ttactgcgag aaatattgct
     3841 aaaaagatgg ggatttcgac acagccgatt tatctggaat tcaaaaacat ggaagattta
     3901 aaaaatacgt tgctggattc tatctttgaa caactaaaag aagatgtttt ccctattgaa
     3961 cgtactggtg ataaaatcat tgatctcggt ttgaactata tctactttgc aaaaaagcat
     4021 catagtcttt acatggcatt gtacattgat gaacatgggg acggaaagaa aatgcatgat
     4081 ttttcataca attatttccg tgaaatagtt aaagaagatg aaagatacaa tcaattatcg
     4141 gatgaatatg tcgatgctct gcatactggt acatgggtag cagtgactgg cttagcgtca
     4201 ttgatgtcat caaacatcat gcatccaaca gaagaacaaa tcattgcact gatcaaacgg
     4261 acaattgact cgattttagc attagataat cctgaaataa tcagatagtg ttctatcagt
     4321 atgaatttga tcggtaagtc cgttggctgc aaactgtcaa tggacttttt tgctaaaaaa
     4381 agcgatttct gttatttatc tattagaaat aggcttgaca tttcgtgaaa aaagtttaaa
     4441 aatagtactt acggaaggtt ggcagagtgg taatgcaccg gactcgaaat ccggcgaacc
     4501 ggctaatacc ggcgcgcagg ttcaaatcct gtaccttcct tatatcaacg attagaggtt
     4561 gtgaagaagc tgtcctgcat caagtaataa gaacggccaa acaaaaatag cttttcctat
     4621 tttttcgttt ggttgggctt attaccgcag atgcaagctt ttgaacaccg tttgttcgga
     4681 ataaggggct gtgacaagac ttttgtcaca gccccttatt gttgtacttt taacctacaa
     4741 tattttttga ttccccctga gtactatttt ttataaatgc tagtaaatca atcttctttg
     4801 gccttttttt tgataaaaaa aaatactaga tgtaagataa aagtgtaaaa acgatttgtt
     4861 ttaggaggga aaacgatgtg taccgcaatc acttatgcaa cgaaagacca ttattttgga
     4921 aggaattttg attatgagat gtcttataat gaagtagtca ctattactcc aagaaattat
     4981 cgtttcgatt ttagaaaggt aaaaaattta gataaacatt atgcaatgat aggaatagca
     5041 gctggggtat ccaactatcc actctattat gaggctacaa atgaaaaagg gttgagtatg
     5101 gcaggactga attttcctgg caatgctgat tataaagaat tacaagaagg aaaagataac
     5161 gttgcaccat ttgagtttat tccatggata ctagggcaat gctcaaccat agacgaagct
     5221 aaagaattat tggctactat caatctagtc aatatcgatt ttagtgaaaa actacccttg
     5281 tcacctttac actggttatt agctgataaa gaaaaatcga tagtaattga aagtatgaaa
     5341 gatggacttc atttatatga taatcctgtc ggtgtgttga caaataatcc gccatttgat
     5401 tatcaattat ttaatctgaa caattatcgc tcgctatcaa atggaacacc agaaaatcat
     5461 ttttcaaacc agatcagctt gaatgtttat agtcgtggga tgggaggatt gggcttacct
     5521 ggagatctct cttctgtttc tcgatttgta aaagccacat ttactaaaat gaatgctgca
     5581 tcaggtgatt cagaatctga aagtatcagt caattttttc atatactagg ctcagtcgag
     5641 caacaaaaag gagtatgcga tactggagaa ggaaaatacg aatatacgat ttattcttct
     5701 tgctgtaacg ttgataaagg gatttattat tatcgtacgt atgaagacag tcaaattact
     5761 gctgtaaata taaataaaga agacttggat cgtcatgaat tggtcagtta tccagtcata
     5821 aaagagcaac agataaacta tgtcaattaa ttaatgaata gatccgtttg gtatcatatt
     5881 ttctgataca gtcataatta gacacgatct ttaggtcgtg tctttttttg ctgttttttt
     5941 tggaaatagc atccactaat atttttttaa cacgaagaga ataaggtagc ctcaaaaaag
     6001 aaaacatggt aaattagata ttgggacttt attttctgta aaaacagaaa ctttcatctt
     6061 ttgttttcac gtgacgaaat tgtaagagag aaaatattcg tccgctattt attctatgat
     6121 aagatgcata tgaatgaaat aaacagtaat tttatattag gatgtgaaag ttaaatgtgc
     6181 ggtattgtag gttttgttaa cgacaaggac aacaaaaaaa caattattaa tacaatgatg
     6241 gatcgaatcg ttcaccgtgg acctaatagt tcaggagaat atatcgataa acatgttgct
     6301 ttaggattca gaagattaag tattattgac ttagagggcg gtactcagcc tatttacaat
     6361 gaagatcgga caaagatcat tatttttaat ggcgaaatct ataattacca gcctttgaga
     6421 gaagaattga ttgcagctgg ccacgttttt caaacacatg cagatacaga agtccttttg
     6481 catggttacg aagaatgggg aacagaatta ttgcaaaaga tccgcggaat gtttgctttt
     6541 gctatttggg ataatgaaaa gaatgagtta ttcggtgcaa gagaccactt tgggatcaaa
     6601 ccatattatt atgcagaaat gaacggaaca tttatgtttg gttcagaaat caaaagtttt
     6661 ctgcctcatc ctgattttaa caaagaattg aacaaagaag cgttgaaacc ttatatgact
     6721 ttccaatatt ctccattaaa cggcgaaaca ttcttcaaag atgtctatcg tttgcctgaa
     6781 ggccattatt atacgtataa agatggaaaa ctagacattc agcaatattg ggatgcagat
     6841 tttgaaacaa aagaaacgca ttcccgacaa gaatggatcg aaaaaatcga cgaaaccgtt
     6901 caagcttcta ttgaagcaca tacggtcagt gatgtagaag ttggttcttt cttatccagc
     6961 ggggttgatt ccagttatgt aacctctgta ttgaaaccag atcattcttt ctcaatcggg
     7021 tttgatgata aaacctataa tgaagcgatt gaagccagaa aactaaccga actattggac
     7081 ttagataata cagcagcagt gatcgacgga gacatgtcgt tcaaagcatt ccctttgatc
     7141 cagtatcact tagatgaacc tgattcaaat ccttcttgcg tacctttgta ctttttagca
     7201 aatcttgctt cacaaagtgt gcgggtagtc caatctggag aaggggcgga tgaactgttt
     7261 gcgggatacc aaacgtatgg attccatacg aattcaaaat tcatccgtgt gattgctcaa
     7321 ggattgaaaa aattaccaaa aggaacacgc tataatttag gacgtaaaat cggaaaaatg
     7381 aaaaatttcc atggacggat ccatttatat gaatcacttg ctcctgcaaa agaatatttt
     7441 atcggtcatg ctcgagtgtt cgaagaatca gaagcatcag aagtgttaac accaaaatat
     7501 caaacagcac catctgtgga tgaaatcatg actgttcatt acgagaaaac agaaggaatc
     7561 aaagatgaag tcaataagat gcaatatgtc gatcttcatc aatggatgcc aaaagatatc
     7621 ttgttaaagg cagataaact ttctatggca agttctttgg aagtccgagt acctttattg
     7681 gatatcgaag tcatgaaatt ggctcaacaa ataccaagta agtttttatt gaatcaaaac
     7741 aacacaaaag atattttcag acaagctgcg aacaaacatc ttccggaaga atggtcgaat
     7801 cgtgtgaaat taggattccc tgtgccaatc aaagcttggt tgaaagaaga acacggctac
     7861 gaacaagtca aagcattatt cgaagcagat tttgcaaaag aattttttga tcaagaaaaa
     7921 atcatgaagt tgctagatga ccatcacgaa ggacgtaaag aagagcaaag aaaaatctgg
     7981 acaatcttca gtttcttgac atggtataaa gtctactttg tagatgaaac gattccgcaa
     8041 gcagaagcaa tcgactatgt gactgtttga tgaaaatcaa atatcaataa aaaagaggtt
     8101 ttctcctacc gtttaacact gagtgataag aaacaatttc aaaaaacagc tcttcccgtt
     8161 ttatagatat tatgacttat ggttcggagt tggcagagga acaccattgt ttcgaacata
     8221 agggggttgt gacacgattt tcgtcacaac ccctttacat atgttcaata atcgatttct
     8281 ttttcttttg tcccatcttt tttataaatg aacaaagttg attctttgtt ttgtgctact
     8341 tgctttcctt tttcgattgc ttcttttttt gtatcaaaat ggttgcttgc cttttttgcc
     8401 cctttagatt tcacgatcca ttgttcttcc tcatattcta cgatcacatc tgaatcaagc
     8461 agttttccag cacgaggatt cgtatcgtgt tcatccgtct tgcttggatt tttttctttt
     8521 tcgaaagttc gtttttctga ttcactagca tcttcatacc atttttttgc ttgatcaatg
     8581 gcaattggga tcgcacggtt atcagggtac ccttcatcta ataatgcatt tgcgatgtcg
     8641 attgcttttt ttcttgtcaa tttatctaag tttttcattg atgcaggaaa gtccttcatg
     8701 ttccatggca tattagtatc cccctttatt aaaaaacata atctatataa acaagtgtaa
     8761 gggtatttat acaaaaagaa aaagattttg tttgagaagt aaaaccgttg tatttatcgt
     8821 caagtagttt tacttaacaa aatcgtgaaa aacactagca atctagaaaa aatcatggta
     8881 agataatcaa gtctgagagt catcttagag gattcttgta aaaatgggag ggaaaaacat
     8941 gcgcgtaaac atcactttag agtgtacttc ttgtaaagaa cgtaactatc taacaagcaa
     9001 aaacaaacgt aacaatcctg atcgtttgga aaaacaaaaa tattgcccac gcgaacgcaa
     9061 agttactttg caccgtgaaa caaaataatg tttttaaagt tctcaattcc ttgctggtca
     9121 aagaattgag acttttttta tctcaaaaca tctaaaatca taaggagcgg ataatatgtt
     9181 tatttccatg tgggcacaag ataaaaatgg tctgatcggc aaaaatggtt tgttaccatg
     9241 gcgattgcca aatgatatgc gtttctttcg cgaacataca atggatcgaa ttttagtgat
     9301 gggaagaaag acctatgaag gaatggggga tctttcactt ccttatcgcc atatcatcgt
     9361 gttgacaacg caaccaaatt ttcgcacgaa agaaaatgcg gaagtaatgc attcgatcga
     9421 tgaacttttg tcgtatagtc aaacgatctc agaagatatc tatgtttcgg gtggtagtcg
     9481 gatttttcaa gaacttcttc cttacactgg tcggatctgg cgaacattga tcgatagcac
     9541 gtttgaaggc gatacgtata ttgggaatat tgatttttca gacttcgccc ttatagagga
     9601 gtatgagggg ataacggatc aagaaaatct atatgcccat cgttttcaaa aatgggaaag
     9661 agtaaaaaat agggttgctt caaaagaaga ataaaatcta tgcgtctagt ttgttaataa
     9721 gaaaaaaagc atctcacgac taatcgtgaa gattcttttt ttagttaaaa tcaatttagt
     9781 ttttctttga tatcattgac catttcctga ctggcaatag gtccgttata tagccaacta
     9841 gattcaggtc cgaattgcag tacatgattg tctgtcactg ctttaagatt cttccagctt
     9901 ggttcattga acatggcagc atctttgtca ctgttcacaa ggatgatgta gtcagcatct
     9961 aattcagcta gcttttcaga ggaaacagca gaccagtcag aagtggcttt tttggagacc
    10021 tcttccacta gatcaggaac accaaattcc agatcgtcgt atacgattct tccacttgac
    10081 cgatttttgc tgaccataaa ggctgagttg ttggtcaccc acaacactgc agctgtcttg
    10141 ttctcgattt tatctgctag ttcttctcga gttttcttga tggatttttg ataatcggaa
    10201 atgatttttt ctgcctcttt ttcttttgcc aaagctttgc cgacatcttt tagttgttct
    10261 tcccatgtga catctgttcc gttttttaca acataagttg gcgcgatttt gctatattgt
    10321 tcgtatttcc cgccttcgac agtagcggaa gaagaaatca acagcaaatc aggctcaaag
    10381 cttaacactt tctcataagg aaggtcataa gaaatcgttg ggacatcctt taaatcatct
    10441 tgcagataat cttgaatgga accactgcca acagtccact gggcgatagg tttttcccct
    10501 agtgcaatca gatagtcttc taagtaactg ccgatgatcc gtttgggcgc gtttggaatc
    10561 tctactttat gtccaagtgt atctgtgact gtatgagtac tattggatga agtcgtcgaa
    10621 ttggcagttg tagctgtttc tttatctgaa gagggggagc aagcgctcag tactccagcc
    10681 agcagcaata tgctagataa tgtgaatact tttgttttta atttcatttc tttcctccta
    10741 acataattga gaatctttct cattggttat tattcctgat atttcgaacg ctgtcaaccg
    10801 cttcatttgg ctatagatat gaaagtagcg cttgacgaaa tgttgaaaca gcgtcataat
    10861 tgagaatgat tttcagcgaa aggatgggtg aaatgacgag attaagagca gctgacattg
    10921 cagccggcta cgaaaacaaa aaaatcattg aaaacatgtc tgttacgatt cctgaaggga
    10981 aaatcactag tttgattggg ccaaatggaa gtggaaaatc tactttattg aaaagtttta
    11041 cccgtatact acctgtgaaa tcaggaaaga tcattgtcaa taactctgat atctctacat
    11101 acagttcaaa agaacttgca aaaaaaatta cattattagc acaaactagt gaacacccta
    11161 caggtatgac agtagaagaa gtagtatctt acggacgata cccttatcaa aaactgttca
    11221 gtggcatgga cacgcaagat catgaagcaa tcgagtgggc gttggatgct acaggtctag
    11281 ctggatttcg tatgcgggat atcgcaacgc tgtctggtgg acaaacccag cgtgtctgga
    11341 tcgctatggc gcttgctcag gaaacagata tcttgatctt ggatgaaccg acaacatttt
    11401 tagatccggc acaccaatta gagatcctac agttattgac tgaaatcaat caaatgaaac
    11461 aaacaacgat cttgatgtcg attcatgaca ttaatcatgc ttcacgtttt tcagattatt
    11521 tattagcaat gaaaaacggt aagttgatca cagaaggtac acctgaagaa gtgatcacaa
    11581 aagagtgtat gaaaaaaata tttgagattg atgcaacgat tgtcacgctt ccaagaacaa
    11641 ataaaccagt ttttctaacc tatgatttgt tgccaaaaga gcaggaggat caattatgag
    11701 gactatgaag aaaaatagac tcctgctttt ggtctgtttt gttcttttga ttattttttt
    11761 tgttgctctt tcaaatggcg cagcgactgt gggaaaagca gatttttggc aggcgttgtt
    11821 tgattttgat agatcaaaac aaagtcacca aatcatacga aatattcgtt tgcctcgtgt
    11881 ggcgggagca tttcttgtag ggagttgttt tgcattgagc ggtgcattga tgcaaggagt
    11941 gacaagaaat cctttagcag attcaggctt actaggaatc aacgcgggtg cttcgttggc
    12001 aatggccctt agttttgctt tttggcctaa agcttctgat ttttctgtgt ttctaatgtc
    12061 attggcaggt tcttttgctg taactttgct cgtatttggt tttttgcagt tttcgaaaat
    12121 gggcatgaat cctttacagc tgattttagc tggcgtagct attagttctt tttttactgc
    12181 tatcagccaa gcacttaccc aattctttaa tctgcagcag gatcttgctt tttggtttgt
    12241 aggcggagct gccaatatca catggaaaca attagcagta ttgtttcctt tatatatact
    12301 tgctgttttg gtttcttttt cattaggaaa agcaatatct ttgattcatt tgagtgaaac
    12361 acatgcatta gctcttggaa gtcatcctaa acgaacgcgg ttgattgttt tctgtctagt
    12421 tgcagtgtta gcagccattg ccgtttcgtt agtcggacct gtttcattta ttgggctgtt
    12481 ggttccgcca gttgttcgcg gaatggttgg aacggactat cgttatgttc tacctgtctc
    12541 ttttttagtt ggcggtggat tggttatgtt ggcggatttt acagcgagga tgatcaatcc
    12601 cccttttgaa acaccttttg gattagtcat tgcattgatt ggcgttccgt ttttactgat
    12661 acaagtaagg agggaacaac ggtgaaaaaa atatattgga cgttctattt cttgcttttt
    12721 gtacttgtca tcctgtctat aggtagttta atgattggta cgccttttat ttacctggat
    12781 cagctttttg ccacgttcat agggaaagaa tcatcttctt tggcgctgat tgttagagaa
    12841 tttcgagttc ctcgattact gatcagttta ttagcaggag cttgtcttgg cgtttctggt
    12901 tatatcttgc aaggagtaac aagaaatgaa ttggcagatt ctagtatatt gggcattaat
    12961 gccggagcag gatttttagt gatgctttat ttaggtttct tttcgcacta ttcgctgccg
    13021 tttctattgc tagttgtagc ctttctaggt ggtgttttag ctgcttgctg tgtctatctt
    13081 gcggcctatg atcgaaatgg ctttttgggt atgaatcgaa tcttattgtc cggtattgcc
    13141 gttaatgccg gcctttctgc attaacattg ctatgcacga tccaattatc taaggaaaat
    13201 tatggatttg tgaatgcatg gttagcggga tcaatctggg gtgcgagctg gccttatgtt
    13261 tgggcattgc ttccatgggc ggttatctta attccgctag gaggacttta tgcacagagg
    13321 attggattgc tatcatttgg tcaagaacgc gcgcaaggat taggccttaa cgtggctaaa
    13381 agtcaaaaaa tccttttgtt gttagctgtt gctttggcaa gcggtagtgt agcggtgaca
    13441 ggtagtttat cttttgtagg actactagct cctcatgctg cgaagctagt cgtacgtaaa
    13501 gaaaataact ggacctttgt tctgagcggt ttgttcggtt ccatttttct gcttagtgca
    13561 gatatggttg ctagagtgat tctaccaagt ggggaagtac ctacaggaat tttgattgcc
    13621 ctttttggtg cgccatattt tctgtatgca ctttttatca gacaaaaaca cgcgctgaac
    13681 agttgaaatt tattaaaaaa ggaaggccaa tcatttctag gtcttctttt tattctattt
    13741 atgcatttat ttgaaagttc gattgctatt tttctttttt tcctatacca ttttatgaga
    13801 gaaggaaggg tgagtttttg gaccaattga aagtaagatt acgccggaaa ggaatcgaaa
    13861 agctgaaaaa tctggccaaa agtggcaaga aaaaggaaaa agaacagcga atctataaac
    13921 agctattcgc cagcgagtac tggaagtcag caaaagtgat agggacgact ttatcgaatg
    13981 aatttgaact ggatacacag ccggttatcc aacgagcaga aaaagaacaa aagaaaatag
    14041 ttgtacctaa aactttgccg aaacgtcaga tggcgtttta tgaaatggat gaacagtccg
    14101 ttcttgtacg ttcgagtttt ggtgttcttg agccacaatc agagaagctc tatttgccgg
    14161 agatgatcga tctcctcatt gtaccaggta ttatctacaa tagagccggt tatcgaatcg
    14221 gttttggcgg tggttattac gaccggtatt taaataattt tcataaaaaa acatgtagcc
    14281 ttgtttttcg tgaacaattg aatgaacaat ggcaaccgga gagctttgat cagaagatcc
    14341 aacaattgtt tattgactaa aatgaaatgg gaggttttca tgaattatca acagcaaata
    14401 aaattgcgtg catggttaag acgaccgtta tggacctatg cttttttagg gatacagaca
    14461 gcggtcttcc tcatgatgga attatttccg cgacttgaga tcccttatta tgccggaatg
    14521 tatggtcctt atcttgttca cttcaatgaa tggtggcgtt tagtgacacc aatcttcatc
    14581 cattttggac tgatgcattt cgtaatgaac tcactgatac tgtattttat ggggcagcaa
    14641 atcgaggcaa tctatggcca ttggcgtttt ttcttgattt atatgttcag cgggataatg
    14701 ggaaatacag caagctttgc ttttaacgaa gcgaatgttc tgtctggtgg tgcgagtact
    14761 tcgatattcg gtttgttcgg tgcgttgttt atcttagggt ttcattttaa acataaccca
    14821 gcgatccagc agctagtcag acattttctt ttatttattg tgttgacatt tgtttttggt
    14881 cttctggata cctctgtaga tgtttgggga catgtcggtg ggctaattgg cggattagtg
    14941 ttagggaatg ttttgggtct accgaaacaa cagacaagct attcgataca ccaaaggatt
    15001 ctttctacac tagtttttgt atttcttttt gtaatatgta ttttactggg tttaaaaaaa
    15061 tatggtttac ttgtataata tgaaggaagt gcttaaagat ggagagctta tatgatgtac
    15121 agcagctgtt caaaaaattt ggcatcttta tctatgtggg tgcacgcatc tatgacattg
    15181 aattaatgac tatcgaattg cgtaatctgt atgatagccg tttgattgat catgatacat
    15241 acatgacagc gtggcgcata ttaaaaagag agcaccgaat tgaagaaagc cgaaagaaag
    15301 gaaatttgta atggacaaga agattattgg gattgattta ggtggaacaa cagcaaaatt
    15361 tgcaatttta acaccagaag gagagattca acaaaaatgg agcatcgata ccaatatttt
    15421 ggatgatggt aagcatatcg ttcctgaaat cattgaatca atcaaccatc gtttgaattt
    15481 atataatatg aaagccgaag actttatcgg aatcggcatg ggtaccccag gaagcgtgga
    15541 cagcgaagct ggaacggtta ttggtgcgta taacttaaac tggacagaag ttcaatttgt
    15601 gaaaaaactg atcgaggcag gaacaggaat caaattcgct atcgataatg atgcgaatgt
    15661 tgctgcttta ggtgaacgat ggaaaggtgc tggagaaaac gatccagacg ttgtattcgt
    15721 tactctagga acaggtgttg gcggtggtat cgttgctggc ggaaatttga ttcacggcgt
    15781 gagcggagca ggcggagaga tcggccatat cactgtggat cctgaagggt tcgaatgtac
    15841 ttgcggtaaa cgcggatgtt tagaaactgt gtctagcgct actggtgtgg tacgtctagg
    15901 tcgttacttg gcagaagaat acgcaggaga ttcaaaactg aaagcaatgt tagacaacgg
    15961 tgaagaagta acaagtaaag atatctttga aatggctcaa gaagacgatc cgtttgcatt
    16021 gatggttgtt gatcgcgtat gcttctactt aggtttagct tgcgccaact tggggaatac
    16081 attgaatccg tcaagtattg tgttaggtgg aggagtatcg gcagcaggag aatttttacg
    16141 tagccgtgta gagaaatatt ttaaagaatt tactttcccg caagtaagag aatcaacaaa
    16201 aatcaaatta gcagagcttg gtaatgaagc tggtgtcatt ggtgctgcct cattagcctt
    16261 gaaatttgct taaaaaaacg gaggcattac atggttttat gggtaattaa tatcattttg
    16321 ctattgattg tgcttgcaat catcttttgg gaagtctatc tacgagtaat ggccaagcgt
    16381 tcagcaacga ctttgacaga agaagaattt cgagaaggaa tgagaaaagc acaagtaatc
    16441 gatgttcgcg aaaaagatgt gtttgatgct gggcatattt taggtgcaag aaacatccct
    16501 tacactgttt tgaaagattc attaggatct attcgaaaag accagcctgt ctatatttac
    16561 gatcaaaaga aaagcttgag catccgtaca gcaaataaat tacgtaaagc aggttatcaa
    16621 gagatttatt tacttaaggg tggatatgac ggatggtctg gaaaaatcaa aagtaaaaaa
    16681 gtataaaaca gatgattgta tgacagcttt aaaaaccgaa tcaatcatta gattttgacc
    16741 aagatcacat gaacggcgca atggatagtt gtgccgttca ttttttttat tttgtgctat
    16801 tatctacata tataagctaa gcgaaaaggc agaaagaagg aaacaactat gatcaaatta
    16861 aaacgtgttt accttgatgc tgaaaaagag gatggctata gaatactcgt ggataggttc
    16921 tggccacgag gaatttcaaa agaaaaagcg cagttagatc tgtggttgaa agaaattgca
    16981 cctagtaatg aagcacgcaa aacttttgac catgtaccag aacgatttgc ttcatttgca
    17041 aagaaatacc agcaagaatt gattttcgaa gaagaaaaga agcaagctat ttttaaactc
    17101 agtcaactta tgaaagaaca tccagtcatt acttttgttt atggtgcaaa aaatgaacat
    17161 cagaaccatg ctgttttatt gaaaaaattt atcgaaatgg aggcatgcaa atgaaaatag
    17221 ggatcattgg tgccggacct agaggattaa gtatagcaga aaggattctc cataataatc
    17281 aggacagaca ttctcttgaa ctttttcttt tcgatcccaa tggtcctgga ggtcgagttt
    17341 ggcggttgaa tcagccaccg gagttattaa tgaattctgt ttctcaacaa gtgacgcttt
    17401 ttacagatga aacattgtcc agtggaggat tggtaacaca gggacccaac ttgtttcaat
    17461 ggagtaaagg acctgctaca gattatatcg aacagacggt aaccaaaaat aagcaactaa
    17521 ttatagaaga aatcaatcgt ctggaaaaag atcagcaatc tactcgatgt ttatacggac
    17581 tctatcaacg ctggtttttc tcaaagttgc aagaagaatt tccagcttct attcagtttg
    17641 tttattcttt agtaaaaaaa gtcagcaaaa gaaaagatgg ttatctttta cagacagatc
    17701 agcaaagcta ttctatcgat cagcttgttt tagcaagcgg tcactgggaa aatgatgaga
    17761 cagaagaaga aagagtgtgg gtagactacg caaagaaaaa tgatttactg tatcagcttc
    17821 cttctaatcc tgcagatgtc gatctttcga agataccgcc taaagaaaat gtctttttaa
    17881 gaggtctagg cttaagtttt tttgattatg tcggattgtt tacagtatca agagaagggc
    17941 gcttcgagaa cgaaaaagga aaacttgtct atcatccatc aggcaaggaa ccgattgtat
    18001 acagtggatc aagaaggggg ctcccttatt accctcgagg aagaaaccaa aagcaaggcg
    18061 gatcaatggc atggccacgg ttactaacaa aagaaaacct ggagcaatgg catcagtcgg
    18121 gatttttgac tggcgaaaca ttttttgact atctgaaaaa agacgttgag ttgttttatt
    18181 acaagaaaat catcgaagaa tatcatttgc cgattccaaa gaaaaatttt gagcatgatt
    18241 ttctctccct ctcttcagag gagtgtctcg gaaagcatcc agcactaatc ccttatagat
    18301 gggattggtc gtatttagaa attcctctta cctatcagga tgagtccttt gcagaagcta
    18361 gccgagcatt tattgaaggg cagataaaag aagcggaaaa aggcaattgc acaggagcat
    18421 ttgcttcaac atttgatgca ttaaaagact ggcgtgatcc aatccggcag gctattgaat
    18481 ggcacgtttt ttctgcaaag gaatacaaag aaatactatg gggatggttt actcatctaa
    18541 atagcttttt aacgatcggt ccgccagtca ttcggacgaa agaattagca gcactgatag
    18601 atgcgggtat ttttcatttg ttcgaaccgc catttgatat tcagcaaaag cacaaccgct
    18661 atgttatttc tctacaagat aaagaaatca gcagtcgcta tttgattgaa gctcgattgc
    18721 cacaaacaag cttttctcgt tcaagtaatc ctgtcataaa aaatttgaag gaaaacaaac
    18781 ttgttcgccc tttttcattt tcagataata caggaagtta tcattcagaa gctatagatg
    18841 ttgagcgagg tacgaatcgc gtgattgatg cttcagggga aatccagtct gacttttatt
    18901 gctatggaat acctgttgaa ggtgtggatt ggctaactgc agcagtagct agaccttata
    18961 cagatccatg gaatctgaga caggcagata aaatagcaca acttatattg actgacaaat
    19021 aaaaatacgc atgatgactt atctgttctt tcacaagtct tcatgcgtgt ttttaaataa
    19081 ttattaacga gaaacgcgtt tgcgcattgc ttttttacgg ttttcttcga tcattgcttc
    19141 tttttcgtca attggatcga tcactgtttt tttcacagaa gcgaccaaac cagcaacggc
    19201 agcggctgtt gccagacttc ctactaatat tccagataca aattttttca ttgaatccac
    19261 ctcatttatt atttgttata tctattatgg aacaaaagag aaaaaaaggc tagttttacg
    19321 tttgtttgta aacctttgca aaaaatcctt gaaaatgttt actaaatgtt ttattataaa
    19381 tttaccatat gaatttacga aattttactt tgtttgtttt caccataaaa gtaaacttcg
    19441 tttttttaaa ttatatataa tcaacaaaca caattttact aatcaaagga gtgtttttta
    19501 tgacaatttt agtactaggc ggagccggtt atatcggttc gcacgctgta gaccagctag
    19561 ttcaaaaggg gtatcaagta gctgtggtag ataatttgct cacaggtcac aaacaagcag
    19621 tacatccaga tgctcatttt tatgaaggag acatccggga taaagaattt ctacgttccg
    19681 tatttgaaaa ggaaccgatt gaaggagtaa tccattttgc tgcaagctct ttagtaggag
    19741 aatctgtcga aaaaccattg atgtatttca acaacaacgt atacggtatg caaatcttac
    19801 ttgaagtcat gcacgagttc aatgtgaata aaatcgtgtt ctcttctact gcagcaactt
    19861 acggagaacc aaaagagtct ccaatcacag aagatacacc agctaatcct aaaaatccgt
    19921 atggtgaaag taaattgatg atggaaaaaa tgatgaagtg gtgtgaccaa gcatatggca
    19981 tgcgctatgt cgctttgcgc tacttcaatg tagccggagc aaaagcagat gcatctatcg
    20041 gagaagatca tacaccagaa acgcatcttg tcccaatcat tttacaagta gctcttggtc
    20101 aaagaaaagc attggctgtt tatggagacg attatgatac accagatgga acatgtatca
    20161 gagattatgt tcaagtagaa gatttgattg ctgcacatat tttagctttg gaatacttga
    20221 aagaaggaaa tgaaagcaac ttcttcaatt taggcagcag caaaggatac tcagtcaagg
    20281 aaatgctaga agcagctaga gaagtgacag gaaaagaaat tccagcagaa atagcaccaa
    20341 gaagagctgg agatcctagc cgattagtcg cttcaagtga aaaagcgaga gaggttctgg
    20401 gttggaaacc tgaatacaca gatatcaaag caattatcaa aacagcatgg gattggcatg
    20461 tcagccatcc aaatggttat gaagaatagg ggaatgagat atgtcaatca gtcaaacgat
    20521 ttgtgatttt gccacacttg cgatcgaagc tggcggatgg atggaaatgg accgcttata
    20581 tttacagaat cgtcttttag ggatggtagg agaagatgct ctagaaagca ttgaagtaag
    20641 accagttaag gaagaatctg tagacttatt ggatcagctt gtcgctaagg caagagaaaa
    20701 tggtgttctt tcagaattgc cagcagaaca agagattttt gaagcgcaat taatggattt
    20761 tctcacaccg ccaccttcag tcgtcaatgc attctttgca cagcactatt cgaaaagccc
    20821 agaggaagca actgagtatt tctttaattt aagtaagaaa aatgactaca tcaaaacaag
    20881 agccatcgca aaaaacatca aatttccggc agagtcggag tatggtgaac tggaaatcac
    20941 aatcaattta tctaagccag aaaaagatcc aaaacaaatc gcagcagaac gcgaggcggt
    21001 aaaagtagac tatcctacct gcatgctttg catggaaaac gaaggatata gaggtcgctt
    21061 gaactatcca gcccgcacca atcaccggat tattcgaatg aatctagacg gtgaaagctg
    21121 gggattccaa tattctcctt atgcttatta caatgaacat tcgatcattt tatcagaaca
    21181 acaccgcccg atgaaaatca caaaggaaac atttgctaga ctgcttcgga ttactgaagt
    21241 cttgcctcat tactttgttg gttcgaatgc tgacttgccg attgttggag gatcgattct
    21301 ttctcatgat cattatcagg caggacgtca tgaatttcct atggcaaaag caccgataga
    21361 aaaacttatc aaactagcag attatccgca agtcatcgca ggagtcgtga aatggccgat
    21421 gtctgtgatc cgtttgcaat caacagagaa aaaattgttg attgaagcag ctaactcagt
    21481 attggaaaag tggaaagcat attcggatga aacagtatct atcagagctt tttcagcaga
    21541 tggtacacca caccatacga ttacgccaat tgcacgaaga agggacaatt actttgagtt
    21601 ggaccttgtc cttcgagaca acaatgtttc agaagaacat cctgatggga tttttcatcc
    21661 acataaagat gtccaacata tcaaaaagga gaacattggg ttgatcgaag taatggggct
    21721 tgctgtttta ccacctcgtt tgaaaccaga actagtagaa gtagagcatt tcttattagg
    21781 agaagaaaac cagatggctg aatatcatcg tccgtgggca gaacaattaa aaaaagaaca
    21841 tcctgatttg acgaaagata cggtcaccga tgtcgtacaa aaatctgtag gtcaggtttt
    21901 tgcacgtgta ctggaagacg ctggagtatt caagcgagat gaaaaaggac aggcagcgtt
    21961 tgaacgattt actgcaattt tataaacgta aaaaaggagg agagacaaat ggcaacaatt
    22021 aaagacattg cgcagttggc aggagtttct cctgctactg tttcaagagt tctcaactat
    22081 gatcctgatc tttccgttgg cttagccaca aagcaaaaaa tttttgaagc agcagaacgc
    22141 ttaaattata cgaaacatcg taaaaatgca gcaaatacta agcaaacgct tcgcctgatc
    22201 cagtggtacg acgatacgga agagcttgaa gatctttatt atttatctat tcgtctagga
    22261 atcgaaaaaa aggcagaaga agcaaacgtt actttattga aagaaacgtg ggattccatt
    22321 agtgatcttc cgacagatgg aacgatcgca ctaggaaaat tcgacgaagc tcagttatta
    22381 gaaatgaaag gatcccaaaa tgccttgctt ttggtagatt ctgacggcac ttctcaagga
    22441 atcgactctc tagttgtcga ctttaagtca agtgttcaaa aagtcattga acattttgtc
    22501 tcccataaag cgcaacatat cgctatgctt gctggaacag aatatacgaa gaaaaaccat
    22561 cagcggctaa aagatcccag aattatggca tttcaacaag ttttggcaga aaaaggctac
    22621 gacgaaggtc caattatcga agcagatttt tctgtcgagt caggatatca agcagtgaag
    22681 cagtatttaa aaacgaatcc gcatgttcct gatgcgcttt ttgcagctaa tgatgcatta
    22741 gcgattggcg cactgaaagc gattcaagaa aaaggtttag tggtaccaga agatatttct
    22801 gtgatgggtt tcaatgatgt cagtgtagcg aaatatgtaa cgccctctct ttctactgta
    22861 aaagtttata ccgaatggat gggagagcta gcagtggaga cgattcttca attaagtaat
    22921 tccaaagcac cggtttcaag aaagattatt gtggaaacag agttgattat tcgagaatca
    22981 acgaaataaa agatggaaaa gctgtgggga aatgaagtga taccgcagct tttttgtgtt
    23041 tcaggaaggg gcaaaatttg cccgccagca gataatcatg tatcattgta ctagttttgc
    23101 ctttggtatt ttcatcctca gaataaaaaa gtatgctaca atggaaggcg agaatgtcat
    23161 agcagaaagg atcaaatata tgattacatt aggaatagat actgcaaacc aaactttggc
    23221 gatcggtgtg gtagaagacg aacaaattct tggacaaata caaacaaata tcaaacgcaa
    23281 ccacagtgta acactgatgc ctgcaatcga ccaattgttt gcagatcttc aaataagtcc
    23341 aaaagacatt gaccgaatcg cggtttctga tggtcccggt tcttatacag ggcttcgtat
    23401 aggtgtaacg acagcaaaaa cgattgctta tacattagat aaagagctag taggagtatc
    23461 tagtctgaaa accatcgcgg ctaattgcat cggtgtggat aaaattattg tgcccatgtt
    23521 tgatgctcga agaaaaaatg tatacgctgg ggcataccgc tggaaagaaa atagattaga
    23581 aattgtactt gaagatgtac atatttctgc ttctgatttg ttcacacaac tacaaaagct
    23641 gaatgaagct atttatttcg tcgggagtga ttgtcacaaa ttcttctatg aaataaagga
    23701 aatattgcct agtgcgatga ttaatacagt tccagcatgg gatattccaa gtggtgtaac
    23761 tgtggctcaa ttaggtgctt tagctgaacc agttgcagag aaacaagctt ttcttcctcg
    23821 ctatttaaaa cgggtagaag cagaagaaaa atggttggag acgcacacac ctggagacga
    23881 aagttatgtt gaaaaaattt agagcgtatg ccagaagctt ttttcaagat aaaatgattt
    23941 atacagaaaa aacaatcgaa caaatcgatc gtcgttacgt actaagagaa ttgactacag
    24001 aagatatcaa agagctgctg gatgtcgaac gagaagtcta taatggcgaa ttgccatgga
    24061 caaaaagtgc ctttatatca gaattgaagt caccgcttcc tcatctatat ctaggtatct
    24121 ttgataatgg aagaatgatt ggttttatcg gttcaagagc gttcggttta gattgccata
    24181 tcacgaatat tgccgttcga ccggcatatc agcgtaaacg aatcggttcg ctgcttattg
    24241 acgaaatcga gaactttgct ataatgaatc gctgtgagac gatgtcatta gaagtgcgta
    24301 tgagtaatca agatgcacaa agactttatc ggaagttagg ttttcaagca acaacgatca
    24361 aaaaaagata ttacactgaa acaaaagaag atgcgttgga tatgataaaa cttttagaaa
    24421 atgagtgaga ttagttgatt tgcagaaaaa aagattttga tgaagcgtac ttggcagaat
    24481 gcgtatggat gattagcgaa aatgcttatc cacatggttc accttggacg aaaatgcagt
    24541 ttttggcaga catccaacag cctcactctg attatctgct tttagttgaa gataaccaga
    24601 tctgtggctt tattggatac tcgaaagtaa tagatgaagt cgagatcaca aatattgctg
    24661 ttgcagtttc agaacaagga aaagggcatg cacggcattt gttacaatta ctgattacgg
    24721 aacaaataaa agattcgctg catgtttttt tagaagtacg tctatctaat gaagcagcaa
    24781 gaaaacttta tgaatcagaa aagtttcgta tattaggtaa acggaaatca tattatcgca
    24841 atccgacgga agatgcgatc attatgagca gaaagttaaa aacggagacg atgaaatgaa
    24901 tgaagaattg attctggcga ttgaatcaag ttgtgatgaa acgagtgtag cggtcgttag
    24961 aaatggaaca gagatcttat ctaacatcgt tgcttctcaa ataaacagtc ataaacgttt
    25021 tggtggcgta gttcctgaag tagctagccg ccatcacgta gaacaaatca ccttatgctt
    25081 ggaagatgca ctggtagaag ctggtgtgtc ggcagaagat ttatcggcag tcgctgtcac
    25141 ttatggacca ggacttgtcg gctcgttgct gattggtatt tctgcagcta aggcttttgc
    25201 ttgggcacac caattgccgc ttattcctgt aaaccatatg gcagggcata tctatgcagc
    25261 ccgtctggtt aaaccattcc agtttccatt gatggcactt cttgttagcg gaggacatac
    25321 agaattagtc tatatgcaag aagatggatc atatgaaatc attggggaga caagagatga
    25381 cgcagcagga gaagcttatg acaaagttgg aagagtatta ggtctttctt acccaagcgg
    25441 aaaagaaatc gatcagctag ctcatcaggg aaaagataat taccattttc cacgagcgat
    25501 gatccatgaa gataattatg atttcagttt tagcggatta aaaagtgcat ttatcaattt
    25561 ggtgcataat gcacaacagc gtggagaaga tctagacaaa aatgatttag cagctagctt
    25621 tcaagcaagt gtgatcgatg tattgattac taaaacactt cgagcttgtc aaaattatcc
    25681 agtcaagcaa ctgattgtag ctggaggtgt agcggccaat caagggttgc gtgaaggatt
    25741 gcaggatgct ttggcaacta aactccctga ggtagaacta gtgattcccc ctcttcgact
    25801 ttgtggagat aatgctgcaa tgattggagc agcagcacat gtggaaatgc aaaaaagaaa
    25861 ttttgctagc tatcagttga atgctgatcc aagcttggtt ttatctgaat aaagaggttg
    25921 tgaaaaatct gccctgcatc aagtaataag aacagcccag gaaaaacggc ttctcatgtt
    25981 tttgacctgg ctgttcttat taccgcgtat gcaagctttt gaacgccgtt taatcaagag
    26041 ttcgtaagcc tgtcgtttaa ctctgattca caaggaacaa tttcaaaaaa cagctcctca
    26101 tgtttatgaa cattatgact tgtgaccgaa gagttgacag gtacacaccg tttaatcgaa
    26161 ataagggctg tgacacgctt ttgtcaaagc cctatttttt acagcttatt catcattatc
    26221 cgttcctctg attgccctca ctgattaatc gtttattgtc gaggaatatc gaataatcta
    26281 ggtcggatga ggacaagacg cttcctctag ttaatctatc agtatttttt gcaaacagtg
    26341 aatattacat aactgaaaca cacatagaaa atctttgagt cttgcccagt gatctaaaac
    26401 tgccggatct ttcagaagtt ttagggagga aaataagcag tgaccctgat aaatatcagg
    26461 gtcactgctt atttttgttc gcgttattct ctcatagctg gagtagtagc tgtcttacag
    26521 aaagtaaaac gctttttgtc ataatctaac agtccggaat cacgtagttg tttgattact
    26581 tgtccagcag tttcacgtgt cgtgccgcta ttcactgcta attcttttaa ggtgatcgga
    26641 taaggaatgg tgatatgacc atcttttgtt ggtacgccca attcttcttt caaaatgaat
    26701 aaattttgtt ttacccggtc aaaggcgcta gaggtcaatc cgatttggat acgcaattca
    26761 tgtatttcaa ctactttaga aattttttgt atcatataga tcaactgttc gggatttttt
    26821 cgtgctaatt tttcatagta ggaaacagga aggtagaatg tttcaatatc agtcatcgct
    26881 tgagctgtat aagtgtaggt ctcttctcca aacattcctg acataggaaa aaggttattt
    26941 gtattcacat agtcatagta gtaataagtt gcactttcat cataacgttc taaacgaacg
    27001 agtcctttgg tcaagatata caaccgttgt ctgggatcac catcgtcgaa aaggatctgt
    27061 ccttttttat aagaacgaag aacagtatgt tccttgatct gttggaactc ttcatccgag
    27121 aaatgttcaa aatcaggttg atatctgagt tttgaaaagt gaaaatcgat tttattaaca
    27181 cgcattaaaa agtcctctcc ttattgttca ctatatttat tgtcactttt tttaaagaat
    27241 ttgtaaagtt ttaac
//
LOCUS       DAZKKW010000033        26839 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.033, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000033 DAZKKW010000000
VERSION     DAZKKW010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26839
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.033"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(40..1146)
                     /locus_tag="ACG8PO_001344"
     CDS             complement(40..1146)
                     /locus_tag="ACG8PO_001344"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HFQ0206682.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVERFDAISDILTGTDSYTDLTSIIQRLQLIKTLEEKDKLIEAGKWADVAF
                     EIGFKAVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGNTKISP
                     NELVLFDLGVVWNGYCSDATRTIAYQKPTDFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLEK"
     gene            1356..2357
                     /gene="ccpA"
                     /locus_tag="ACG8PO_001345"
     CDS             1356..2357
                     /gene="ccpA"
                     /locus_tag="ACG8PO_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001228 - DNA-binding transcription
                     activator activity, RNA polymerase II-specific [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HFQ0206683.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            complement(2694..5039)
                     /locus_tag="ACG8PO_001346"
     CDS             complement(2694..5039)
                     /locus_tag="ACG8PO_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HFQ0206684.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLMFAA
                     LGIGIGAGYFAYLVEDTQLPTKKALQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIILSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAK
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSDAYYQKAQQTIQNKGYTIHSTIDKDVYAALQNGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLIGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINAKITTPFKDAISSLNGNLGKA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSKARIRSANYLANLINQAY
                     QAKPDIFGTDEKFELSSDVMKKKVSGFTGQTPGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANQATKESDEKDD"
     gene            5318..6220
                     /locus_tag="ACG8PO_001347"
     CDS             5318..6220
                     /locus_tag="ACG8PO_001347"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HFQ0206685.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAYMAEKGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGTEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKIIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQTFDPEVFPELLAEEVIL"
     gene            6433..8196
                     /locus_tag="ACG8PO_001348"
     CDS             6433..8196
                     /locus_tag="ACG8PO_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HFQ0206686.1"
                     /translation="MLNTLKPLINQLKKKHTYFILGIALLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIRYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVPLTQF
                     IVVTSMLVFSKLNLKKALSSK"
     gene            complement(8262..9848)
                     /locus_tag="ACG8PO_001349"
     CDS             complement(8262..9848)
                     /locus_tag="ACG8PO_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HFQ0206687.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVSQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQNEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHAALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQLLQETVEEIE"
     gene            10099..10323
                     /locus_tag="ACG8PO_001350"
     CDS             10099..10323
                     /locus_tag="ACG8PO_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HFQ0206688.1"
                     /translation="MLSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            10465..12216
                     /locus_tag="ACG8PO_001351"
     CDS             10465..12216
                     /locus_tag="ACG8PO_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HFQ0206689.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGVFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIIFGWIFLLIIAAFLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     QMWEKQQLEAKIEGGEA"
     gene            12216..14000
                     /locus_tag="ACG8PO_001352"
     CDS             12216..14000
                     /locus_tag="ACG8PO_001352"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0206690.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGMYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYIRVKLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFLAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     VLTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSIFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQMITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGI"
     gene            14326..15177
                     /gene="licT"
                     /locus_tag="ACG8PO_001353"
     CDS             14326..15177
                     /gene="licT"
                     /locus_tag="ACG8PO_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
                     /protein_id="HFQ0206691.1"
                     /translation="MLIEKVLNNNVVITRDERNQEMIVMGKGLAFKKKVGDPIFDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEALQVQLNDSVYIALTDHLY
                     TAIERHKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTITIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GEKSEVGEVSEELFQVIQKKYKDTFGCVEKIADFLAETFNYQMSTDEKMYLTIHTARL
                     VHENRCK"
     gene            15270..17156
                     /locus_tag="ACG8PO_001354"
                     /pseudo
     CDS             15270..17156
                     /locus_tag="ACG8PO_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782556.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
     gene            complement(17196..18098)
                     /locus_tag="ACG8PO_001355"
     CDS             complement(17196..18098)
                     /locus_tag="ACG8PO_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFQ0206692.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQAIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIHDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSAMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVE"
     gene            18329..19732
                     /locus_tag="ACG8PO_001356"
     CDS             18329..19732
                     /locus_tag="ACG8PO_001356"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008706 - 6-phospho-beta-glucosidase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFQ0206693.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPAMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEENGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKEEGIEVQ
                     DELARELSGNTHIMEEV"
     gene            19732..21165
                     /locus_tag="ACG8PO_001357"
     CDS             19732..21165
                     /locus_tag="ACG8PO_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFQ0206694.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGKDRFPV
                     ITGKKKMFEFDEDHFYPAKIAIDMYHRYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNEAGLKFYEDAFKECQKHGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEKGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIEMEPGDAALLKEHTVDFISFSYYASRVSTTDNELLEQTAGNIFASV
                     KNPYLQASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNQGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            21310..22236
                     /gene="trxB"
                     /locus_tag="ACG8PO_001358"
     CDS             21310..22236
                     /gene="trxB"
                     /locus_tag="ACG8PO_001358"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFQ0206695.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYESVSRFGTENAYGIVQDIKDHGSHKEVICEDKSYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNMPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            22450..24177
                     /locus_tag="ACG8PO_001359"
     CDS             22450..24177
                     /locus_tag="ACG8PO_001359"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HFQ0206696.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAKHSGLITIIGEEVDAAYLEEIKAV
                     TIDRELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPDGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYFAEKTISVTMSGQEGSAKISALMEKFRKEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGSSTEDAAEKVASYE
                     AIIKKMTEE"
     gene            24192..24542
                     /locus_tag="ACG8PO_001360"
     CDS             24192..24542
                     /locus_tag="ACG8PO_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFQ0206697.1"
                     /translation="MEKVVNFRSQSFFKLEVNGLPDTQKSKVEKVSQLFKMLSDPTRL
                     KILLYLKDGEQNVTAITQAVEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHV
                     LDILNQTLKHVEHQ"
     gene            complement(24634..25494)
                     /locus_tag="ACG8PO_001361"
     CDS             complement(24634..25494)
                     /locus_tag="ACG8PO_001361"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HFQ0206698.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLRQALQDALEEKALVLHTLINQELIEITRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEKSD"
     gene            25586..26014
                     /locus_tag="ACG8PO_001362"
     CDS             25586..26014
                     /locus_tag="ACG8PO_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFQ0206699.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            26176..26352
                     /gene="rpsU"
                     /locus_tag="ACG8PO_001363"
     CDS             26176..26352
                     /gene="rpsU"
                     /locus_tag="ACG8PO_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFQ0206700.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            26379..26825
                     /locus_tag="ACG8PO_001364"
     CDS             26379..26825
                     /locus_tag="ACG8PO_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HFQ0206701.1"
                     /translation="MSLLSRLNDDMKAAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
BASE COUNT         8542 a         4786 c         5673 g         7838 t
ORIGIN      
        1 aaaaaggtgg gagcggacta gctctcacct tttttactct tatttttcta aaacaagtaa
       61 ttcttttggt gtagtcgtga atggcacaca gccatcgctt gtcacatata cacagtcttc
      121 gatacgtacc cctacttttt caggaatata gattcctggt tcaagagaga agcacatccc
      181 ttcttcgatt actaagtcat ttccttctac aagggaaggg aattcgtgga cggttgttcc
      241 gatcccgtga cctaaacggt gattgaaata ttcgccgtag ccatagctgt tgatcacgtt
      301 tcgtgcgatt tgatcaagtt cacctgctgt cacacctgga cggacggctt cttgtgcggc
      361 aagctgtgct tcaagagtga tgttgtagat tttttcttgg aagtcggttg gtttttgata
      421 agcaattgta cgagtagcat cactgcaata gccattccaa acaacgccaa gatcgaataa
      481 cacaagttca ttcggtgaaa tcttcgtatt tcctggtgta ccatgagggc ttgcagcatt
      541 tgctcctgca aggactaatg tgtcgaaaga catttgtgaa acaccttgtt ttttcaattg
      601 gtattcaatt tctgcaatga tcgcttgttc agtcacgcct tctttcactg ctttaaagcc
      661 aatctcaaat gcaacatcgg cccatttacc agcttcgatc agtttgtctt tttcctctag
      721 agttttgatc agttgtagac gttggatgat ggaagttaga tcagtgtaag aatccgttcc
      781 cgtcaagata tcagagatag catcgaagcg ctcgacagtc aaggcgttct tttcaatccc
      841 tacattgcca ggctgaccat agcgagcacg aagttctttt ccgatgatct cccaagggtt
      901 ttcgctgtct aaatagccac ggacatcata cgcccaacta ctgttttgag catcttcaac
      961 ttccaaagca ggagtgaaaa ggaaaggatc ttgttcaaga gggataaaaa gagctagtac
     1021 acgctcatga ggatcacttt cgtatccgga aaagtaagcg atgtggccag ggtcgctgat
     1081 ataagccaat tcgacttggt gttgtgccat ccattgacgt agttcattga tttttacgtt
     1141 attcatttgg caacctactt tctaaattaa ttttgtattc aataattgta ccactgattt
     1201 attaaaagac caacgttcta aaaactgaaa aaagtaaaaa ttttcatgaa aacagagata
     1261 aaacggttgc aattcttgag attatttgat attctatttt agaaatgtaa gacgtatttt
     1321 cagaaaacat ataaaatatg aataggagaa cacaaatgga aaagcaaaca atcactatct
     1381 atgatgtagc gagagaggca aatgtttcga tggctactgt ttcacgtgtc gtaaatggca
     1441 atccaaacgt taaaccagca acaagaaaaa aagtcttgga agttatcgat cgcttggatt
     1501 atcgtccaaa cgcagttgcg cgtggcttgg caagtaagaa aacaacaaca gttggtgtaa
     1561 tcatccctga tgtcagcaat atgttctttg cttctttagc acgtggtatc gatgatgtgg
     1621 caacaatgta caaatacaat attatcttag caaattcaga tggcaacgac caaaaagaag
     1681 tcaatgtatt gaataactta ctggctaagc aagtggacgg cgtgatcttc atgggtcacc
     1741 atatcacaga cgaaatccgt ggagaatttt cacgttcaaa aactcctgtt gtattagctg
     1801 gctctattga tccagatgaa caagtaggta gcgtaaacat tgattatgta tccgcaacaa
     1861 aagatgcagt aaacaaattg gcaaaaagcg gcaataaaaa aatcgctttt gtcagtggtg
     1921 cgttgatcga tcccatcaat ggacaaaatc gtctgaaagg ctataaagaa gcattaagcg
     1981 aaaataacct accatatagc gaaggattga tctttgaagc acaatacaac ttcaaagatg
     2041 gtctatctct agtcgatcgg atccataata gcggtgcaac agctgtctat gtttcagatg
     2101 atgaattagc catcggtatt ttggatggtt tattagaccg tggcgtgaaa gtaccggaag
     2161 aattcgaaat catcacaagt aacaattcac tactgacaga agtagctcgt cctcgtttaa
     2221 caagtatcac acaaccatta tacgatttag gggcggtatc tatgcgccta ttgacgaaaa
     2281 tgatgaataa agaagaagtc gaagaaaaaa cgattatctt gccttatgga atcgaagaaa
     2341 aaggatctac aaaataataa aaggacaacc attcagaggt tgtgtgcctg ccgtttaact
     2401 ctgcgttaca aggagcaatt tcaaaaaaca gcatctcatg tttgatgaat attgtgactc
     2461 gtgattcgga gttggcagga gaacaccgtt tatttgggaa aaggggttat gacaaaattt
     2521 tgttataatc ctttttttgt gtcttaaaaa agagagaaag tggttgaaat tacatttatc
     2581 gtatataaaa caatccgtgt atagatgctg cattttgttc accaccatca aaaaaataag
     2641 ggactgtgac aatactattg tcacaatccc ttattactgt gtattctatt aaatcaatca
     2701 tctttctcat ctgattcttt agttgcttga ttcgctcttg cataagctga agcggtgttc
     2761 cagtaatctg tatagttttc atcggtaccg ccaacgccaa atttaaaggc gcttttcttc
     2821 ggtccatttt tcgcccatag cgattcaact gtttttcctg gtgtttggat ggatttttta
     2881 ttgacagtaa cttttccagg tgtttgacca gtaaatccag atactttttt cttcatcaca
     2941 tcagatgata attcgaattt ctcatcagtg ccaaaaatgt caggttttgc ttggtaagct
     3001 tgattgatca gattggcaag ataattagcc gaacgtatcc ttgctttgct gtccattcca
     3061 gtattgtcat cgtgccctgc ccagctactg atggtaatag acggagtaga gacaattagc
     3121 catgaatctc ggtattcatt ggtagaacca gtttttccga cccaatctgc ttttcctaga
     3181 tttccgttta agcttgagat ggcatctttg aatggtgtag tgatttttgc attgatcaca
     3241 ctacgcatca tgtcattcat gatcgatgcc gttgcttccg aatagatccg gacagagttg
     3301 ctttcgtgtt tataaattac atttcctgcg ttatctgtga tggaatcgat gatatagcct
     3361 tcttcataga cgccgccgtt tgctaaagcc tgaaatccgt tggtctgttg aagcgtcgtg
     3421 acatctactg tacctaaagg agcagattca acgccccaat tgtcatttgc cgggtagttc
     3481 atctttgcca agtagtgctc gtaagcatat tgtttcgatc caccggaatt caacacatct
     3541 tgatataagt gataagccgg gatattgttg gaccattcta gtgactcacg gacggtttgg
     3601 aaggtgttcg atccttcgtt agtagcattg acgattttct cgccagcatt ttctccttct
     3661 tgccaagttg ttgcataatc tgatacacgg gattctgatc cgataagccc catgtcgatt
     3721 gcaggtccat aaacaagaac tggtttgatt gatgagccag cttgacgttc tgtatcgaag
     3781 gcatggttat tttgattctg actgtaattc cgtccgccaa cgaatccgta gatgcggcca
     3841 gttctgttat ccatcaatac atttccggtt tctacttgac tgccagcacc atcatctaac
     3901 atgtatccat aattttcaac gccattttgc aatgcagcat agacatcttt atcgattgtc
     3961 gaatggattg tatatccttt attttggatc gtttgctgtg ccttttgata ataagcgtca
     4021 ctgaccgatg aatcagacat atcagcgttg tctttttcag ccaattgttg tgcaatgatt
     4081 cttgttgctt cattcatcac agtataatac aagaattctc tgtcagtctg ttctgcgatt
     4141 tgttgtggta gaaagtcttt tgtaaggtca taggcttttg cttcttcgta ttgttctttc
     4201 gtaatttggc cttcacgata catactgaat aaaacaaaat cttttctggc aaggccggca
     4261 gaaagatctt cttttagcgc gcctgtgttc gtgtatggac tatacgtgat cgggctttgg
     4321 ggtaatccag cgatataagc agcttgcggc agcgtcacat cttttgcatc tacaccaaag
     4381 attccttttg cagcttcctg gactccagca atgttttgtc ccttattatt acgaccaaat
     4441 ggtgagacat tcaaatacgt agcaatgatc tcatcttttg aaaagtattt ttctacttgt
     4501 gcagaaagaa tgatctcgtt tgctttacgt ttgaatgttg tttcatctgt aaggatctgc
     4561 tgtttgacta attgctgtgt caaagttgat ccaccagaag agccaatacc agtagcttca
     4621 gatactaatg ctcgtaaaac agctttagga acgtaacctt ggtgtttatc gaagtattca
     4681 tcctctgtgc tgataattgc agttttcaat aaaggtgaaa tcttatcaga tgaaatagtc
     4741 gtgcgcatca gatctgtctg gatttttgat atctctgtat tatctgcata aacaatcttt
     4801 gacgtttctg taatgtttcc tagttctgtc tgcagtgctt ttttagtcgg caattgtgtg
     4861 tcttcgacga gataggcaaa atagccagcg cctattccaa tacctagagc tgcgaacatc
     4921 aaaagaagaa cagtaataaa aacagtcaat gattgaaaaa ctctaaaaat aatcttagga
     4981 acaaaccatt tttctttttt tcttctattc tgtttttttc gagtattttt gcgtggcaaa
     5041 agggtcacct caactaatgg ttttatttcg ctaattatag caaataatag aacaaaaaaa
     5101 cagacaaggc tgtttttggc aggaatttct tttttaaaag agttaaaacg tgagaaaatg
     5161 atctttatta taaaagagtt cttaagcttt tctttagaga aaatttcagt tactatggag
     5221 aacagggcaa agaaaaatca atcattttct ttgtatatca tcttaccaat atataggaat
     5281 tgttagtaac aattgccttt caaggaggaa tccatccatg ttattaggct cacacgtcag
     5341 catgagcgga aagaaaatgc tgttaggagc agcagaagaa gcagccagtt atggatcttc
     5401 gacttttatg atctatacag gcgcccctca aaacactaga agaaaagcca tcgaagagat
     5461 gaatattgat gccggaaaag cgtacatggc agaaaaaggt ctaacaaata tcgtcgttca
     5521 tgccccttat atcattaatt tggggaatac gatcaaacca gaaatgtttc cttttgccgt
     5581 ccaatttctg agagaagaga ttgttcgtgc ggaagcttta ggcgctactc aaattacact
     5641 tcacccaggt gctcatgtag gggcaggaac agaagccgga attgctagaa ttattgaagg
     5701 cttgaatgaa gttttgacaa aagaacagac ggcacaaatc gctttggaga caatggcagg
     5761 taaaggtacg gaaatcggtc gaacatttga ggaattagcg caaatcatcg atggagtaaa
     5821 attgaatgaa aaattatccg ttacttttga cacctgtcat acgaatgatg ccggttataa
     5881 tattaaagaa gacttttcag gtgtaatgga agagtttgat aagatcatcg ggttagatcg
     5941 gcttaaagta ctccatatca atgattctaa aaatccgcaa ggttcgcata aagaccggca
     6001 cgccaatatt ggttttggaa caatcggatt cgacacgctg aataagatcg tccatgatcc
     6061 aatgtttgac aatatttcga aaatcttgga aactccctat gtaggcaaag ataaaaagaa
     6121 agcttacgca ccatatggaa aagaaatcgc catgttcaaa gcgcaaacat tcgatccaga
     6181 agtgtttcct gagttattag cagaagaagt gatactctaa gattttctat ctgacagcag
     6241 caaaaaagga ccaggcacat tgccttgtcc ttttttgttt aagaaacaaa ggagtacttt
     6301 ttgcagtctt tttcatcatg catcttctcc cccctctttt taaatttaga caaacaaggt
     6361 atactggtag aaaagtaaaa aaacgaaaag tttcacaagc aagtggcaaa acgtgattgg
     6421 aaggacgaaa agatgctgaa cactttaaaa cccttgatca accaactaaa aaagaaacat
     6481 acatacttta ttcttggcat agccctatta tttacaatta ttcgtttgta tttatatagt
     6541 aaagcaatct atggaacaga catgtcggca acctatgatg accagctgct gattcgctat
     6601 gctcaaaata ttttggacgg taaatggcta ggggagtata cgactacaac actttccaaa
     6661 ggaatctcct acagcttatt tttagtactt gcacataaac tgcatctttc ttatgcagtt
     6721 ttgttcggca tgttcagtat ttttgccagt atattggttg cactcgcgtt taaaccgatt
     6781 gtgaagaaca agtatcttct tttaagtatc tatttttatt ttttgttttc tccgattagt
     6841 tttacacatg aatattccac tcgaacatat cgtaatgccc tcgtgattcc ggctgttgag
     6901 atcattatcg cttgtttatt agctatttat taccgcaaat acggttctag cagaaaattg
     6961 ctgccatggt ttttagggct ttctgtcgct tttcctttct tttggttcat tcgcgaagat
     7021 tctatctggc ttcttccttt tactgtagta gctttgggta tcagtttcgt ccagatcttg
     7081 ctagggcaag acttttcttt caaagaagta aagaagttaa aaaaacgact ctttcttctc
     7141 tcgaaacggg aatggctgaa actttcattg tttttacttc ctatagttgg cttttccatc
     7201 gtctatcaat ggatcgaata taaaaatgag acaacatacg gtattgcaac catcaatgac
     7261 cgttccagtg gagaatttgg acgtttgatg aaacaactga tccgaataga tgatgaaacg
     7321 aacttgaata aagaaaacag tgagatatgg gtctcacatg ctgcactaaa taaagcatta
     7381 gccgcatctc ctacattcca tactgtttct acccgtattg acgaattata tacgactcac
     7441 gcgtggactc aaggtggaaa agtaaaagaa ttacccggag atatcatctt ttgggcattg
     7501 cgagatgtgg taagcgaatc tggttattac caaaacaatg caaaagaaac caatgatttt
     7561 tggaaaaaag tcaaccaaga attagaagaa gcctatcaca agggaacaat caaaaagaaa
     7621 aaagagttct atctgatggg atcaggagat ggaaagacga tgagtgatct gccaatcgta
     7681 ttcgatttcc ttcaatcagg atacgcttat gcatggcgat ataaagattt ccatcaagga
     7741 ggaaattata gtttcggaac gcaagaacaa gtcaaacagg ctcaagaatt attaaacctt
     7801 ccgctaatgg agaattggat cgatagtaat gaaccgagaa cacctaattt ccaattaaca
     7861 aaatcagcaa aagtcgcgaa tgtcatcata aaaatttacc aagttctggc tattcccgtg
     7921 ctgattgctt caatagcagg gatattactt atgatactag gaaccgtatt tgcaaaagct
     7981 gatagttcct ctttgattgt ggtcaccgga ttaatactga cacaattggt ttttcttttc
     8041 ggtgtctcat ggttttgttc ttatgcgcca gaaaaaaagg actatttttt aagtgtctac
     8101 actggtgcag gcgtgccatt aacgcaattc atcgtagtaa caagcatgct agttttcagt
     8161 aaattaaatc tgaaaaaagc tttatcttct aaataaaaag acccttggta gctagatttt
     8221 taacctaact atcaagggtc tttgtgtgga aaaatcagtt tttattcgat ttcttctacc
     8281 gtttcctgca aaagctgttc atattttttg tttcctttat ataaatcacc aatcgtccat
     8341 gccaacagga gaaggacaag aacaatgatt acataagcga tgtgatcagc caatgctagt
     8401 tgagcggaag tggcttgctc gccgaagaaa ctttcgatct gtcctggtaa tcctcgtaag
     8461 ttcaagtaag ttagactaat cacagagaac cagccaagaa gttttacgat gaatgaattt
     8521 ttaaatcggc tacccatctc cgcttggctg ttggtcatca tcagtaaagg aatcatcgaa
     8581 aacggcaacg caaaagccaa gaacacctgg gattcattca ttaagttatt caaagcggca
     8641 tgttcttcga tcgttccttt tgtacttgta aacaaaacac aaatcaatac aggaatcaca
     8701 gaaagcaatc gtgtgaccag tcttcttagc caaatcggga tacgcatatg gatgaatcct
     8761 tccatgatga cttgaccggt caacgtacca gtaatagtag agttctggcc agaagcaaga
     8821 agtgctacag caaataaagt ggacaagata ccagattttg ctacagcaat caaaatacca
     8881 ttgctcatag ctgatggatc agataaagct tcatacagac caaaaaagga aggatcttgg
     8941 acagaaccgg ttttgaagac agcgacaccc atgatcaata gcagtgcatt gactagaaaa
     9001 gccattgaca actggatatt agaatcccaa gtagaaaaac gtacagcttg tgctacatct
     9061 tcttcgtttt ggtgatcgat cttccgagtt tgtgaaactg ctgaatgaag atataagtta
     9121 tgcggcatga ctgttgcacc aataatccct aaagctccag tcagcggagt ttcgccgccg
     9181 atgaccggtg agtctgagaa tgtatgactg ttagggataa agccagcaaa taaactgtgc
     9241 caatctgggt tagacaagcc aacttgatag acaaaaacga ataaaatgac gaaaattaag
     9301 cagacaacca gtgcttcgat cttacgaaac ccgacttttg tcagtagtaa gagaacgaaa
     9361 acatctaata cagtaataaa gacggcaatc accaatggaa tatcaaaaag taagtagagg
     9421 gcgattgctg caccaatgac ttctgcaata tctgttgcca taatggctaa ttcagtcaag
     9481 atccataaaa tgacacctaa tgtcttactt gtgcgtgctc ggattgcctg tgccaaatcc
     9541 atttggctca cgattcctaa ttttgctgcc atatactgta aaagcatagc aatcagacta
     9601 gacatcaaaa taactgacat caataaatat tggaaatttt gtcctccggt gatcgaagta
     9661 gaccagttac ccgggtccat atacccaact gctactaacg caccaggtcc agagtaagca
     9721 agaagtgttt taaaaaaact tttccccttt ggtacatcaa ctgttccatt tacttcagct
     9781 aaagataaac catttgtatg ttcaataagt cgatgcttat gaaattgttt attggattga
     9841 ttattcaaga aaaaaccacc tttcctattt atctacgatt acattctaca cctcaaaaag
     9901 aaaaaattaa agaaaaagat tcggtacgcc taattttttt tcatggcgcg tcttgctaga
     9961 tgacgaaaga aattttgttc tcctctaaaa aaaccactat tttttgcatg aaactaaaga
    10021 atacgccttt cttttttcaa taaactttag taaaataagt cattgagagc aattcggaaa
    10081 tagaaaggag agatttatat gttatcaaca ggtattgtcg tacttattgc tattattgct
    10141 ttgctaatcg gcgcagtagg tggctttttc ctagcgcgta aatatatgaa agactattta
    10201 gaaaaaaatc ctcccgttaa tgaggacatg ttgcgatcaa tgatgatgtc aatggggcaa
    10261 aaaccatctg agaagaaaat tcgccagatg atgcagcaaa tgaagaacca agggaaaaaa
    10321 taggttctga cccaacgact ttttacgggc tgattttttc agtccgtttt ttatttcgta
    10381 atttgttaga ctattcagat tattcagcaa aaaagacaag cacagacaat cctaggctct
    10441 tgataaagac aaaggaggag taaaatgtcg atttttagaa aattaggctg gttttttcgt
    10501 caggaaaaga aacattattt aattggggtt ttctcgctat tcgcagtggc cttggtacag
    10561 ttagttcctc caaaagtgat tgggatcatc attgatgaga tcgccgataa aaatttatca
    10621 ataaagatca ttttcgggtg gatcttccta ttgattatcg ctgctttttt acaatatctt
    10681 tttcgttata tctggcggac gaatatttgg ggaagtgcag ctcgattgga gaaagaactt
    10741 cggcagcagt tattcaaaca ttttacagaa atggacaatc tcttttacca aaaatatcgg
    10801 actggggatt tgatggctca cgcaacgaat gatttgaatg cgatccagaa tgtagcagga
    10861 gctggtatct tgacctttgc agattcagtg attacgggcg gaacaacgat cattgcgatg
    10921 attttgttta ttgattggcg cttaacactg attgcgctga ttcctctgcc tttgctggcc
    10981 gtcacttcac gagtgctggg gtcaaaactc catgatgctt ttcgtgattc gcaagaagct
    11041 ttttctagta tcaatgataa gacacaagaa agtatcactg ggatcaaagt cattaaaaca
    11101 tttggacaag aaaaagaaga tctagcggat tttgaagaaa agattgaaga tgcaattatt
    11161 aaaaacaaac gagtcaattt tttagactcc ttatttgatc cattcattac cttgattatc
    11221 ggtctgtctt atgttgcaac aattatttta ggtggaaagt atgtcttgga aggcacgatc
    11281 tcgatcgggc agatggtctc atttattagt tatatcggta tgcttgtttg gccgatgttt
    11341 gcgattggtc gattgttcaa cgtattggaa agaggaaatg cttcttatga tcgcgtcaat
    11401 gaacttttgc atgaaaagac gcatatcgtg gaacaactgg gtgcagtgca gaccccggca
    11461 aaaggcgagc tgaatgtagc tatttcctct tttaattatc cgaaagaaga atcaccagca
    11521 ttagaacaaa tcaagtttgc gatccaagaa ggagaaacat taggtattgt tgggaaaact
    11581 ggtgcgggaa aaacgacgat catcaaatta ctgatgcgtg aatacgatga ttatcaaggg
    11641 aaaatccaat ttggcggacg ttcgatcaaa gaatattcat tgaatgcttt attaggatca
    11701 atcggctatg tgcctcagga tcacttcttg ttttcgatga ccattcgaga taatatccgt
    11761 ttttctaatc caaaactttc agaagaagaa gtagagcatg cggcttcttt agcatttatc
    11821 aacaatgaaa tcaaggaatt accaaaaggt tatgatacct tagttggaga acgtggagtc
    11881 tctttatctg gcggacagaa acaaaggatt tcgattgctc gagcattgat caaaaatcct
    11941 gaattattaa tattagatga tgcactctct gcagtagatg ccaaaacaga agaagctatc
    12001 ttgcataatt taaaagaaat aagaaaagag aaaacaacta tcattgcagc tcaccgttta
    12061 agtagcgtga tgcatgcaaa agaaatcatt gttgtcgatc aagggaaaat cattgaacga
    12121 ggaacccatc atgagctgct tgcactaggc ggctggtatg aacaaatgtg ggagaaacag
    12181 caattagaag cgaagataga aggaggcgag gcctgatgga tgaaaattat caatcggaat
    12241 ggacaaaatc gattccactt aaagaacaga aaaagatcat caaacgactg atgaagttcg
    12301 ccaaaccttt tagaagaacg ttcatcgttg ctatcttatt tgcgtttgct ttgtctgtga
    12361 tcaatgtcct attgccaagg atcatccaaa cttttatgga tgatcatctg gctaaacagt
    12421 cggctacgac acaagttatt ttgttttttg ctggtatgta cttatttgga gtcatagtaa
    12481 aaagtatcat ttggtttttc caatggtatt tgtattccat ggcctcattg aagacctatc
    12541 agtatattag ggtaaaatta tttgaaaagc tgcatacact aggtatgcgt tattttgatc
    12601 agacaccggc aggatcaacc gtttctcgag tgacgaatga cacagaaacg ctatttgaat
    12661 tttggtacgt ctttttgatg gtattgactg ggatctttgc agtcgtttct tcattccttg
    12721 ccatgtttca aatcaatgga gaaattgcct tgtggacttt ggtgtttttg ccgattttag
    12781 gaatcgtcat ctggtattac cagaaattca gttctcgtat ttaccgaaat atgcgggaaa
    12841 aattaagtca gctgaacacg aaattgaatg aatcgatttc cggcatgcgt attatccagc
    12901 agtttcgcca agaagctcgt ttagctaaag aatttgaaca agtgaacaat gagtatttag
    12961 acacgcgtta tgcgatgatc aaaacgaatt cgattctgtt aagtccgatc atcaattttc
    13021 tatatgcatt ggcgattgcg ttggtgttga ctttgtttgg gatcgatgcg ttgcattctc
    13081 ccgtagaagt tggactgatc tatgcattta cgacctatgt ccagggattt tttaatccaa
    13141 tgtctcaaat gatggacttt ttgagcatct tcacagatgg gatggttgct ggtagtcgta
    13201 tcttgaagat tatggatacg gaagaatatg caccacaaca aaaccctgga gcagatcaaa
    13261 tgattacttc aggtaagatt gagtttcgca atgtaagctt ttcttatgat ggagagcaca
    13321 atgtattaaa caatatctct tttgttgcta atccgggaga gaccgttgca ttagtgggac
    13381 acaccggaag cggaaaaagc tcgatcatca acgtattgat gcggttttac gaattttatg
    13441 aaggtcagat attgattgat gatcatgaca ttcgagaata tccaatggaa gagttacgta
    13501 agaaaatggg gctggtcttg caagatgctt tcttgtttta tggggatatc gcaggaaata
    13561 ttcggatgat gaatccagct atcacagacg aacagatacg ccatgcggct gagtttgttc
    13621 aagcaaacaa attcatcgat gaattaccaa aaggatatca ctcaaaagtg atcgaacatg
    13681 gtgccagcta ttctagtgga caacgacagc tgatcacttt tgcgcgaaca atcgtgactg
    13741 atcctaaaat tctagtattg gatgaagcaa cagcaaatat cgatactgaa acagaaggat
    13801 tgatccaaga aggattggca aaaatgcgtc aaggacggac aacgattgcg attgcccatc
    13861 ggctgtcaac gattcgagat gcagaattga ttttagtttt agacaaaggt catatagtag
    13921 aacgcgggaa tcatgaaaca cttctagcgc aaaatggttt gtatgcagat atgtacaaac
    13981 tgcaaagcag tggtatataa aagatgttgt gacagtagaa ttttgaccta agcactagag
    14041 tgaaaataaa acatagcttc tcatattttt catctgtgag agttagtgtc gtagtcacca
    14101 gatttaaaaa aagattcgac agctagtcag aagtgcctaa tagccactaa gaaagcgttg
    14161 acaaaagcat ttgctttcga taatataaag aaaacgatac aacttggata gtgattgcct
    14221 gatgcaagct aaacctgatg agtctcccat ggtttcatgg acggcttttt gggtttttct
    14281 ttttgttttg gatacagccc tatacatcat ggaaaggagc aaaacatgct gattgagaaa
    14341 gtactgaata acaacgtcgt aataaccagg gatgaaagaa atcaggaaat gatcgtgatg
    14401 ggaaaaggtt tggcattcaa aaagaaagtg ggtgacccga tttttgactc atgcatcgac
    14461 aaaaaatttc agttgacaaa tgacgggctt tcacaacagt tccaagaatt ggcacaagcg
    14521 attcctcttg agtacttaga aatttcttgt gaaatcattg aatatacaaa agaggctcta
    14581 caagtccagt tgaatgattc tgtgtatatt gcattgacgg atcatttgta tacggctatc
    14641 gaacgtcaca aaatgggaat atttgtacca aatgtattgc tgtatgatat caaacaacta
    14701 tttcctaaag agtatgccat cgggaaaaaa acagtgaaga aaatctcgga taaataccac
    14761 atcaagttat cagaagatga agccggattt atcgcattgc atttagtcaa tgcacaaggc
    14821 attcaaagcc agcaagacaa tacctacctg tttactataa caatacagga tattctaaat
    14881 atcgtccgct attactataa gattacattt gatgaagatt cagtccattt ttttcgattc
    14941 gtcacacact tacgtttttt tctgacacgc gtcctagatg gagaaaaatc agaagtggga
    15001 gaagtttctg aggaattgtt tcaggttatt caaaaaaaat ataaggatac gtttggttgc
    15061 gttgagaaaa tagcagactt tttagccgag acgttcaact accagatgtc cacggacgaa
    15121 aaaatgtatt taacgatcca tactgcgcgt ttagtgcatg agaatcgttg taaataaaaa
    15181 aaggaggata aattaacaag aacgtatgaa ataagggtag tgacattcag ttggctaaac
    15241 cttccactaa taaattggag ggaatagaaa tgggaaaata ccaagaatta gcgagaaaaa
    15301 tcgtagaaaa tgtcggcggg aaggaaaata tcaatggttt gacacattgt atcacaaggc
    15361 ttcgtttcaa attaaaaaac gaagaaaaag caaatgatga gatcttgaaa aatatggacg
    15421 gtattgtaac ggtaatgcga gcgggagggc agtatcaagt tgtcattggg aatcatgtgc
    15481 cagtcgtgtt tgaagaggtt ataaaagcag gaaaccttac ttttgatgag gctgtttcaa
    15541 cagaaaaaat gagaccgttt gatatgctta ttgatggaat atcgggctgt ttccagccat
    15601 tccttgctat cctagctgct ggcgggatga ttcgtggact gacagcattc cttgtcttct
    15661 taggcgcgtt tgaccgcggt tctggcacat ttgttatgtt tgataatatc ggtgattctg
    15721 tattccaatt tatgcctgtt attatcggtt taacagctgc ccgtaaattt aaagtaaatg
    15781 aattcgtagg gatgttgctt ggcgcagcat taatgaatcc tagcttgtcg ctggaagctt
    15841 tgagtggtgc agcagaagca ccactaacga ccatattttc tggcactatt tttgaagcac
    15901 ctatttatca aacagtattc ggcattccct ggattgcgcg taactacgct tcttctgtca
    15961 ttcccattat ctttattgtg ttgttagcta gccaaattca gaaaccaatt aaaaagttag
    16021 tacctgagat gatcgcgaat ttcttcgttc cattcttcac cgttcttatt acaatgcctt
    16081 taggtttttt actcgttgga ccagtattca cattcgcaac tgatatttta atggctggat
    16141 ttgaaacact tttagcactt tcgccagtta tttatggagc aatcgtaggc ttcttctggc
    16201 aaattctggt gatgtttggt ctccattggg caatcgtacc aatgggattg atgcaatttt
    16261 ctgtgaatgg ttggcaaaat attatgacac ctgttgcagt agtgtctttt ggacaaactg
    16321 cagcacttac cgcactctac tttaagctta gaaatccaaa agacaaagca attgcaatac
    16381 cagccattgt ttctggtatt gtggggatta cagagccagc tatttatggg tttacattac
    16441 cacgtaaaaa aatatttatt tttacctgtg tcggtggtgc gttagggggc gcgtatagtg
    16501 ggttaatgaa tttaacttct tggaatcaag gtggactagg catctttaca attcccaact
    16561 atattagacc agatggtgat ttaacagatg tgattaatgt attgattggg atcgccattg
    16621 cgatggtggt atcctttacg ctgacttttt tcttttggaa ggatgaagca ggtgaaacag
    16681 atgatataca gaaaaaatca ggtaaagaaa ttgtaaaaac accgatacaa ggacaaatcg
    16741 ctccactaaa tgcagcaaag gatgcagctt ttgcacaagg aacactaggt cgaggaatct
    16801 tgatctaccc tgaaaaagga gaagtacgtg ccccttttga cggaacagtc atgaccctgt
    16861 tcccgacgaa acaggcaatt ggaatggttt ctgaaacagg gctggaattg ctgattcatg
    16921 tcggattgga tactgtgcag ttggaaggga aatattttga aagcttagtg cagcaaggat
    16981 aaagagtgaa acaaggcgat ctgttgctac gcttcgacaa aaagaaaatc gaaaaggaag
    17041 gatacagttt agaaacacct gtgatcgtca ccaattacac ggattatttg gatattttgg
    17101 aaaaccaagg ggagacggtc gggccagacg atgcacttat caccgcactg acctgaacgg
    17161 ttaggtgaag aatggactag gcttaactat tctttttatt ccacttcttt attttcaaaa
    17221 ttgaaaggct ctttcatgat ggctgagctg atagggcgtt tatcgaattt ttgaccgaca
    17281 gaaattagat ggactaaatt ctgttgataa ttttctgcaa aaacacaact atataacacg
    17341 tctagaagat aacaaattga tgtattgatc gtaaaattac caatttttga atacagtcgc
    17401 tcgcgtgtcg taagtcgcaa agcggctgta ctcattgcag atagcgttga atcaccgata
    17461 ctggtcaaag ctaaaattgg aacatgattt tgtttcaata tttctgcggt acgaaggatc
    17521 acattgtttt cacccgtata agaaatcaga atcccgcaca tatcactcgt gcaattgtac
    17581 gcttcataga atgattcacc aaaagtggtg acaatcgtga catccatttt gatgcgcttc
    17641 attttcagca tgaaatcttg agaaatcaaa gtattcgagt tactggcgaa aatcacgatt
    17701 ttttttgctt gaagcattag ttgtttggct ttttgcagtt ggtcatgatg aatgagagaa
    17761 agtgtatcat gaatcgtcat ctgttctaaa gaagccattt tatttgcgat cgtcataata
    17821 ccgtctgtgg tggaaaaagg aagattcgca tctacgtctt gaaaattccc ttcaagatag
    17881 gctgtttcag ctaaaaaagc attacgaaaa ctgacccagc catcaaaacc catttttttt
    17941 gctacacgga tgagtgtaga ggggtggaca taacatcgct cagcaatcgc ttggatggtc
    18001 atttcgccaa gtgctggaga atcgcttaag agaaactgga tcacttcatt ttctgaagga
    18061 gaaaaggaga tttctttgat tttttcacgt aataacatgt agataccttc ttcttatttt
    18121 tttacgtaaa ttataacttt tttgaaaatg atttgtaaac ggatttatac tatataactt
    18181 aaaatagaca ttttttgaca agatatttac aaaaatccac tagaaaacag taaattttgt
    18241 aaatgttact ggaaagaaac agatttctat tgtttgtttt gtttacaaaa aagataatga
    18301 aagtgtaaaa aagtaaagga gtgagaacat ggcaaaagga atcaaaatcg ctacgattgg
    18361 tggaggcagc agttacaccc ccgagttgat ggaaggattt atcaaaagat atgaggaatt
    18421 accgatacga gaaatctggc tcgtagatgt agaagaagga aaagaaaagc ttgagatcgt
    18481 tagtcagatg tctcagcgta tgtgggatgc ttctccgtat gaagtcaaga tctatgcaac
    18541 tctggatcgg gaagaagcat tgaaagatgc ggattttgtt acgactcagt ttcgtgtcgg
    18601 tcaattagat gcacgaatca aagacgagcg gatccctagt tattatggga tgattgggca
    18661 agaaacaaat ggtgctggcg ggatattcaa agcttttcgt accgttccag tcgttttggc
    18721 aatcgttgag gacatgaagc gtttatgtcc aaatgcttgg ctgatcaatt tcgcgaaccc
    18781 aagtgggatg gtgacagaag cagttatcag atatggcaaa tgggataaag tgattggatt
    18841 atgcaatgtg ccagtgatgg cgatgatgac agaaccagca atgcttggca agaaaaaaga
    18901 agagctgatc tacaagtttg ccggtctaaa tcatttccat tggcacaaag tagcagataa
    18961 taaaggaaat gaccgcacgg cagaattgat tgatcgttta tacgatgaag agaatggatt
    19021 accgaaaaat atctttgata tcccgttttt caaagaacaa ttaaaacaaa tgaacatgat
    19081 cccttgtggc taccaccgct attactatcg tgaagaagaa atgctggaac atgctttaga
    19141 agaatatcaa acgattggta caagagcgca gcaagtgaaa gaaacagaag cagagctatt
    19201 tgaattatac aaagatccag aattagacca taaacctgaa caattgcaaa aacgaggtgg
    19261 cgcttattat tcagacgctg cctgtgaaac gattgcttca atctatggaa ataaagaaac
    19321 acagatcgtg gtatctacaa agaatgatgg agctgttcct gacctgccgg cagattgtgt
    19381 agtagaagtc actgcctatg ttggcgcaca aggagcgaga aatgtagcgt ttggttctct
    19441 tcctgctgct gaaagaggct ggttgcaagt aatgaagaat atggaattgc tgacgatcga
    19501 agcagctgtg actggtgatt atgggacagc tttacaggcg tttacgatca atccacttat
    19561 acgatcagga aagacagcaa aacggatcat ggatgagttg tttattgcac accagcagta
    19621 tttaccgcaa ttcaaggaga caatcagccg attaaaagaa gaaggaatcg aagttcaaga
    19681 tgaactggca agagaattat caggtaatac acatataatg gaggaagtat aatgggattc
    19741 agaaaagatt ttttatgggg cggtgctaca gcagccaatc aatgtgaggg cggttatgat
    19801 gaaggtggac gcggactagc aaacgtagat gttgtgccaa tcggaaaaga ccgttttcct
    19861 gttatcaccg gaaagaaaaa aatgtttgaa tttgatgagg atcattttta tccagcaaaa
    19921 attgcaatcg atatgtatca tcgttataaa gaagatattg ctttgtttgc agaaatgggg
    19981 ttcaagactt atcgaatgtc catcgcctgg agtcgtattt ttccaatggg ggatgagaaa
    20041 gagccgaatg aagctggctt gaaattttat gaggatgcct ttaaagaatg ccaaaagcac
    20101 ggaattgagc cacttgtgac gattacgcat tttgattgtc caatgcattt gatcaaagaa
    20161 tatggtgcgt ggcgttccag agaactcgtt ggtttttatg aaaatctgtg tcgagtcatc
    20221 ttcaatcgct ataaaggact tgtcaaatat tggctgacat tcaatgagat caatatgatc
    20281 cttcatgcac cattcatggg cgcagggctg tattttgaaa aaggagaaaa tgaagaacaa
    20341 atcaaatatc aagcggcaca tcatgagttg gttgcaagtg ccatcgcaac gaagattgct
    20401 catgaagtag atccggagaa caaagtaggc tgtatgctgg cagcaggagc atattatcca
    20461 tacacttgca atcctgaaga tgtttgggct tctagaaaat cagatcgaga aaattatttc
    20521 ttcattgatg tccagtcacg aggagaatat cccgcttatg cattgaaaga aatggagcga
    20581 aatggcattc agatcgaaat ggaaccaggg gatgccgcat tgctgaaaga acatactgtt
    20641 gattttatct ctttttctta ttatgcttcc cgagtttcga ctaccgacaa cgaactcttg
    20701 gaacagactg caggaaatat ctttgcttct gtcaaaaatc catatctgca agcaagtgaa
    20761 tgggggtggc aaattgatcc attaggcttg agaataacga tgaatgatat ttatgatcgt
    20821 tatcaaaaac cattgtttat tgtagaaaac ggcttaggtg cagtcgatac gccagatgaa
    20881 aatggttatg tggaagatga ttaccgaatc gattacctgg cagcgcatat ccaggcgatg
    20941 aaggatgcag tagaactaga cggagtcgat ctattaggct atacgacttg gggatgtatt
    21001 gatttagtat cagctggtac cggtgagatg aagaaaaggt acggattcat ttatgtcgat
    21061 cgtgataacc aaggaaatgg cacattagcc cgttcaaaga aaaaatcatt caactggtat
    21121 aaaaaagtaa ttgattcaaa tggagaaaat ctttcgaacg attgatcaat gatttataaa
    21181 agtctattgt ttttcagcgg atttttcgag tataataaag ctattcgaga aacgcaagca
    21241 gaacaataga ctttttttcg cattggctgt aaagtatgct tacttgatta agtaaaggag
    21301 aatctacata tgtatgatgt aatcgttatt ggcgcaggtc ctgctgggat gactgctgct
    21361 ttatatgctt cacgttcaaa cttatccgta ttgatgatcg aacaaggtgc ccctggcggc
    21421 caaatgaaca atacggcaga aatcgaaaat tatcctggat tcgattcgat catgggcccc
    21481 gatctagctt ataagatgta tgaaagtgtc tcaagatttg ggacagaaaa tgcttatgga
    21541 atcgttcaag atatcaagga tcacggcagc cataaagaag tcatctgtga agataaaagt
    21601 tacgaaggaa aaacagtcat tatcgcaaca ggatgtatcc accgtaaatt aggcgtacct
    21661 ggcgaggaag aatttgctgg acgaggcgtt tcttactgtg cagtctgtga tggtgcgttt
    21721 ttccgtaaca aacgtctagt agtcatcggg ggtggggact cagcagtaga agaagccatc
    21781 tatttgacac aatttgcttc tgaagtagtg atcgtccacc gtagagatga attacgtgca
    21841 caaaagatca tccaagaccg tgcatttgca aatgaaaaaa tttcctttat gtgggataca
    21901 gtcgttgacg aaatcgttgg aaacgaaatg gtagtgacag gtgtgaaagc acgtaatgtc
    21961 aaaaccggca atgaatttga tttagcagca gacggagtat tcatctatgt tggactggat
    22021 ccattaactg caccttttag agaagcaggt ttgacgaacg aagctggctg gattccaaca
    22081 gacaacgaaa tgaaaacgaa tatgccaggc gtttttgcca tcggagatgt tcgtgaaaaa
    22141 gatttgcgac aaatcactac agctgttggt gacggcggta ttgctggaca acaagtgtat
    22201 aaatacttgg agtctttaga agaaacaaca gcataagttt aaaaagagta gaagaacggg
    22261 ttcaattgtt cttttactct tttttttcgc gtgaaattta ctgaatctga tttatgcgaa
    22321 cacgtccaac accttttgat aaaagaatcc tttcttgatt taagcgtttt ttggtcctca
    22381 tactttcata tctaataagt gaatgctata cttattaaga accatatttc agaaagaggt
    22441 gttattttca tgtcttggga acaagtgtat gaacaatggg tacatgaaga aaatttagaa
    22501 gaaaacttaa aaaaacaatt aaatgatctg ggtgaagatc ctgaaaaact tgaagatgct
    22561 ttttatgcac cgttggaatt cggtactgca ggtatgcggg gaatcttagg acctgggatc
    22621 aatcgaatga acatctatac ggtaagacaa gcaacagaag gactagcacg tttcatggat
    22681 acacaaggtg aagaaacaaa acgccgaggt gtcgctatcg cttatgactc acgccatcag
    22741 tcaccagaat ttgcaatgga agcagccaaa acactagcac atcacaatat cccgtcttat
    22801 gtattcgaaa gtctgcgtcc aacaccagag ctttcttttg cagttcgtca tttgaagacg
    22861 tttgcgggaa tcatggtaac cgcatctcac aacccagcgg cttacaatgg atataaagtc
    22921 tacggagaag atggcggaca aatgcctcct gcagacgcag atgcattgac gaaatatgtc
    22981 cgtgaagtca gcaatccgct aaaagtcgaa gtattatctg acgaagaagc taaacatagc
    23041 gggttgatca cgatcatcgg agaagaagtg gacgctgctt atctagaaga aatcaaagca
    23101 gtgacgattg atcgtgaatt ggttgaaaca atggggaaag atttgaaact tgtgtacacg
    23161 ccacttcatg gaacagggaa aatgttgggc gaacgtgcgt tgaaacaagc tggattcgaa
    23221 caattcgtac tagttcccga acaagctgtg gcagatccag atttcactac ggtcaaatcg
    23281 ccaaatccag aagaacattc agcttttgaa tatgctatac gacttggaga aaaagaagga
    23341 gctgacttgc tgattgcaac agatcctgat gcagaccgtt taggcgcagc ggttcgtttg
    23401 ccagatggtt cttatgaagt attgactggg aaccaaatcg gagcattgtt tgctcaatat
    23461 attttggaag cacatcaatc aagaggcact ttaccagaaa acgcagcggt attgaaatca
    23521 atcgtttcca gcgaattgcc tacagcaatt gcggaacact acggtgctac catgttcaat
    23581 gtgttgacag ggtttaaatt cattgctgaa aaaatccaac aatttgaaga agaccattca
    23641 catactttta tgtttggatt cgaagaaagt tatggttatt tagttaaacc ttttgttcgc
    23701 gataaagacg cgatccaagc attgctttta ttagcagaag tagcagctta ctataaaaaa
    23761 caaggcaaaa cgttgtatga cggcttgcaa acgatttttg aaacctatgg ctatttcgca
    23821 gaaaaaacga tttctgtgac aatgagcgga caagaaggtt ctgcgaaaat ctctgctttg
    23881 atggaaaaat tccgtaaaga agcaccgaca gaatttgccg gagcaaaagt tattcaaaca
    23941 gaagacttta aagaattgac gagaacagac cgtgaaggaa acgtagaaaa actaacaaca
    24001 ccgccatccg acgtattgaa gtatacattg gaagaaaatg gctggatcgc tatccgccct
    24061 tcaggaactg agccgaaaat caagttttat attggtgtaa aaggaagctc tacagaagac
    24121 gcagccgaaa aagtagcctc ttatgaagcg attatcaaaa agatgacaga agaataaaaa
    24181 acttgagact gatggaaaaa gtggtaaact ttagaagtca gtcttttttc aagttggagg
    24241 tgaatggatt gccagacacg caaaaatcaa aagtagagaa agtcagtcaa ttattcaaaa
    24301 tgttgagtga tcccactcgt cttaaaattc tgctatatct gaaagacggg gaacaaaatg
    24361 tgacagcaat cacccaagca gtggaaatgg agcagtctgc tgtatcgcat caactacgtt
    24421 tattaagaga aaatcatgtc gtcaagtctc atcgcgaggg aaaagcgatt ttgtatagtc
    24481 ttgatgattc tcatgtactg gatattttga accagacact caaacatgtg gaacatcaat
    24541 aacgataggt gaaaatagat aaacactgga gtgtgacaac gaagaaattt cgttgtcaca
    24601 ctccagtgtt ttttaagaaa aagacaaaaa tgattaatcg cttttttctg ttgtttcagt
    24661 tccataataa ctatgatctt ctaggtctaa agcattcaaa ataagcgaag ccgtttcttc
    24721 aatagataaa gatgccacat tgatcactac acatcctagt ttttcataaa gatcattcgc
    24781 aaaatcgagt tcagctctaa ttttttcgat atcggaataa gcagtatcag ggtttaatcc
    24841 gtaagagcgc atccgttctt tacggatatt atttaaaata tctgggttat tcgttagtcc
    24901 cacgattttt ttaggatcga tttcccacag ctgtttggga atatgtgctt ggggaatcaa
    24961 aggtaaatta gcaactttta aatttttatt tgctaaaaac aagctcaaag gcgttttgga
    25021 agttcgggat acgcctagca aaacgacatc tgcttctaaa aaaccacgag gatctttccc
    25081 atcatcgtat tttacagcaa attccattgc tttgatccgt ttgaaatagt tctcattcaa
    25141 taaatggagg gcaccaggtt gccgaattgg cttgatacca gttaaacgtt caatttctga
    25201 tatcggtggc gtcatcacat cgaaactaaa taattgatgt tcttcacaaa aagaacgtgt
    25261 tatctcgatc agttcttgat tgattaatgt atgcaggaca agtgcttttt cttctaatgc
    25321 atcttgcaat gcctgcctta atgttttttc atctgtcacg aacgttctgc ggaataggtt
    25381 gaactcaacg gtaggatact gagccatagt agcttgcgct agttttgtcg cagtttctcc
    25441 agcagagtcg gagatgacaa aaaaagtaac gatttctgta gaatcggaag acatggataa
    25501 aacctcactt gcattttttt tatgtttatt ataccatact ctagaatgcg tacttcgtaa
    25561 tcattttgat ttggaggaat aagtcatgaa aaaacagaca ttgatcgaac aatcattaga
    25621 aacattgaaa aataacggtt ttaaatatac taaaaaaaga gaagcattgt taacttattt
    25681 agtgaagcgt aaccgctacg tatctgctaa agaagttttt gactatatga atcaggaatt
    25741 caaaggggta agttatgata cgatttaccg gaatctccat gattttgcag agatcgattt
    25801 actggaagaa accgatttaa atggtgaaat gaagtttcgt tttcattgct gtcaggggga
    25861 aatcggccat caccatcatt ttatctgtac agtgtgcgga acaacgaaag aaatccatat
    25921 gtgtccaatg aattatttcg aagaacagct aggtggatgc acgatcgaag ggcatagatt
    25981 cgagattttc ggaagatgtg aaaattgtca ataaggattt aaataaaata acatttgata
    26041 atttaaaaaa aaagcaagat ttccttgacg gtcaaaacag cattagatat aatatttaca
    26101 ggacagtctt gttttttgag atatatttca ataacaatta cggttacttt ttaagctcgg
    26161 agggagggaa ttaacatgtc aaaaacagtc gttcgcaaaa acgaatcact tgacgatgct
    26221 cttcgtcgct tcaaacgttc cgtgtcaaaa gcgggtactt tacaagaatc tcgtaaacgt
    26281 gaattctacg aaaaaccaag tgtaagacgc aagaaaaaat ctgaagcagc tagaaaacgc
    26341 aaaaaattct agttctgtga tgaattggag ttggtagtgt gtcactacta agtagattaa
    26401 acgatgacat gaaagcagcg atgaaagcga aagataagga atctttacaa gtcatccgaa
    26461 tgatcaagtc atctatacaa aacgagcaga ttaaagaagg tcacgacttg acagaagaag
    26521 aagaactaac ggtgttgtct cgcgaaatga aacagcgacg tgattctttg catgagtttg
    26581 aagaagcagg acgtgacgat ttagctgaaa aagtaaaaag tgaaattgta atagtcgaaa
    26641 aatatatgcc ggaacaactt tccgacgaag aaattcgaca acttgttcaa gaagcaatca
    26701 ctcaaactgg tgcttcatca atgaaagaat ttggcaaagt tatgggtgca attatgccta
    26761 aagtcaaagg aaaagcagat ggaaaccaag tgaatgcgat tgtgaaagaa ctattacaat
    26821 cttaatttta tcatttaaa
//
LOCUS       DAZKKW010000034        26430 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.034, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000034 DAZKKW010000000
VERSION     DAZKKW010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26430
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.034"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            40..1380
                     /locus_tag="ACG8PO_001365"
     CDS             40..1380
                     /locus_tag="ACG8PO_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFQ0206702.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGIILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHAADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFAAYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLIIFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLTLRKGTLW
                     DKGISFFALAVLWLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            1575..2018
                     /locus_tag="ACG8PO_001366"
     CDS             1575..2018
                     /locus_tag="ACG8PO_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HFQ0206703.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINAMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILTSKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(2280..3698)
                     /gene="nhaC"
                     /locus_tag="ACG8PO_001367"
     CDS             complement(2280..3698)
                     /gene="nhaC"
                     /locus_tag="ACG8PO_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFQ0206704.1"
                     /translation="MEKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKKYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDILEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPKLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(4102..5325)
                     /locus_tag="ACG8PO_001368"
     CDS             complement(4102..5325)
                     /locus_tag="ACG8PO_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFQ0206705.1"
                     /translation="MTTTLSTNQLKDLIQYRLPEYQALALAIHDHPEVSNYEFYSSAA
                     LIKQLEKEGFWVEKEVAGHRTGFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDEIGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDAALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAALSTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSEKGLASIAIAAELLANTALDLLREPKLLEKIKQE
                     HQEIVARKKQEGATF"
     gene            complement(5357..6196)
                     /locus_tag="ACG8PO_001369"
     CDS             complement(5357..6196)
                     /locus_tag="ACG8PO_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFQ0206706.1"
                     /translation="MKKIIRFAVITFVAIIWLAGCSQGKAATTEKKETTVKLGIIGED
                     TDVWDDVKDRLKKENIHLEYVKFTDYNQPNAALADGSIDLNSFQHQFFLDNYNEEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENKKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDEHARPYVNIIVARKEDEDNKTYQKIVDAYQTEETAKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(6213..6893)
                     /locus_tag="ACG8PO_001370"
     CDS             complement(6213..6893)
                     /locus_tag="ACG8PO_001370"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFQ0206707.1"
                     /translation="MTEFFKTYLPNVYLIPDEFIEATKQTLYMSFWTAFIGGIIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILVLVFLSQFISN
                     QLIKKVTH"
     gene            complement(6895..7968)
                     /locus_tag="ACG8PO_001371"
     CDS             complement(6895..7968)
                     /locus_tag="ACG8PO_001371"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFQ0206708.1"
                     /translation="MIELRDISVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEAGK
                     IIEKGSSIQIFSHPEEELTKDFIRTATHLDQALETISAHAAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRQKALDYLIKS
                     GVKVTILQKNELDNRIKVIEGGN"
     misc_binding    complement(8027..8265)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    complement(8308..8541)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(8618..9289)
                     /locus_tag="ACG8PO_001372"
     CDS             complement(8618..9289)
                     /locus_tag="ACG8PO_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFQ0206709.1"
                     /translation="MKGAIFDLDGLLVDTERTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVQAAGSPEKVQEVRAKREEYFYQQLTSGAIQLKPYAKEILTDLKERHM
                     KLGIATTTVTKRATDILDHFQLADYFDTFTFGDEVSENKPSPVPYLTALERTGLEALS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYSVEEKEKLNLLAEGKDLRTVIEMLD
                     KKTEK"
     gene            complement(9377..12025)
                     /gene="mgtA"
                     /locus_tag="ACG8PO_001373"
     CDS             complement(9377..12025)
                     /gene="mgtA"
                     /locus_tag="ACG8PO_001373"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HFQ0206710.1"
                     /translation="MEKKKDTQKIYAAFAGKTKQELFELFHASPNGLSEEQIERSRET
                     YGENAISYGKKTPFIIEILKAYITPFTLVLIALGIISFITEYVLAAPGDKDLFGVAII
                     FTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGEYTEIPMEEIVCGDLVRLS
                     AGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSYENLVFMGSNV
                     ISGSAEGIIVSVGNDTLFGHVAETLSEKPIQSSFELGIHKTSMLLIKFMSLMAPAVIV
                     INGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGTVIKNLNAIQN
                     FGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQTGLKNLLDVAI
                     IDEAAETLDTDTINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGAIEEMLNISKF
                     IDLGGTVAPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFSVKDESDMVLI
                     GYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLEINELITGEKI
                     SEMNDHELAQVAEEHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGINDAPAMKVADI
                     GISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKATASSNFGNMFS
                     VLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKWNASSIGKFMV
                     YFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQQVIFIALFHAGWFVESLWTQT
                     LVLHALRTPKIPFIQSNATFAMLVITTLGIIVGSVLPFTGFGAELGLMPLPGNYWLWL
                     FITVVAYLALVTAVKKIYIRRFQELL"
     regulatory      complement(12368..12542)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
     gene            12715..13152
                     /gene="ndk"
                     /locus_tag="ACG8PO_001374"
     CDS             12715..13152
                     /gene="ndk"
                     /locus_tag="ACG8PO_001374"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFQ0206711.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFATMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKGADAVPGTIR
                     GDYALPGTENIIHASDSRDAATKEIARFFPPFDTNKPSEKAAK"
     gene            complement(13174..14040)
                     /locus_tag="ACG8PO_001375"
     CDS             complement(13174..14040)
                     /locus_tag="ACG8PO_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0206712.1"
                     /translation="MYDGELIKKLRVNKKLTQTQLAEGICSKTSLVGIETNSVKKVSF
                     GTLKSFLERMNMTLAEYEWIRNQLDEPKKLKKKRRMLDRIQEDSFDPYKEIANNRRHY
                     KKTEDLYYLVLNLHMSKKTEVSETEFEFLRYECRIIEEYLMQVPEYGQFEVSILADFP
                     WIFSDRFIENNYLKIKKRMRRIAQLNNHEQYLFTFLTNLTLFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKSENHQEFFAVIEYTLGKNQRNSL
                     EKKLLAIAKPSS"
     gene            complement(14274..16352)
                     /locus_tag="ACG8PO_001376"
     CDS             complement(14274..16352)
                     /locus_tag="ACG8PO_001376"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFQ0206713.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVSKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPATLGQTEGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTIL
                     YPVTGTNAEQ"
     gene            16845..18062
                     /locus_tag="ACG8PO_001377"
     CDS             16845..18062
                     /locus_tag="ACG8PO_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFQ0206714.1"
                     /translation="MPYILALDVSMGHSYSVLYKDDTCLFEDEIEHTQRGFHALLEEI
                     QHLPERPLIVFEATGIYSRPVEKFFQDHHFSYCLLNPLEAKKQMEENTLRSWKTDKSD
                     AHRLAQTHLKNQRQPKEVQSNFYLEMRDLARFYQEIEKKITRLRMDLHNCLQLTFPEL
                     EQFFSNRLTPYALTLIRLFPHPDFVLASTRTKIKNKLINETREKISANRAEQKADQII
                     HYAQCAYPAVEKDSIHCQKTIYYAELLQDLLEQKKALATQMIKKAEASPCFLLYQTFP
                     GIGALTAALLLGELGDITRFKTHKQLNAFVGIDIRRYHSGKYTGQDRINKRGNPKARK
                     IIFFTIRNMIRQQRAAPNHIVDYYYKLKKQPIPKKDKVATVACMNKLLKCMHAMVRAH
                     TEYDYAYAVSVDH"
     gene            complement(18521..19144)
                     /locus_tag="ACG8PO_001378"
     CDS             complement(18521..19144)
                     /locus_tag="ACG8PO_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0206715.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWEEYKLTANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLVVLLLTHDGDKVARLKNGKVVTAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     ASSQKNESTELSPSDSGSGQTITGNEENQASIITNESSEGQYQTYGQ"
     gene            complement(19300..20697)
                     /locus_tag="ACG8PO_001379"
     CDS             complement(19300..20697)
                     /locus_tag="ACG8PO_001379"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFQ0206716.1"
                     /translation="MKRYQFPKNFWWGSAASGPQTEGRISGDGKGENIFDYWYKNEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HHYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFQLFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKAYHEMDQSGVI
                     GIILNLTPSYPRDESNPEDVKAARIADAFFNRSFLDPAVKGEFPSELIERIKELDLMP
                     EIHPDDLTIIKENTVDLLGINYYQPRRIKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEKGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDRNGFEA"
     gene            complement(20719..21690)
                     /locus_tag="ACG8PO_001380"
     CDS             complement(20719..21690)
                     /locus_tag="ACG8PO_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0206717.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMEKISEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISNRFGSEVVSGIERVTEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHAIDGLIVFLSEEEWSGFEMLTKMSIPVVALNCPGGPAGIDTIVVNDR
                     ESVAKVIAHHGGQGHKTIGFIAAAQTDYVARERLAGAKEAVQKDPSIHLEIFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            complement(21697..23997)
                     /locus_tag="ACG8PO_001381"
     CDS             complement(21697..23997)
                     /locus_tag="ACG8PO_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HFQ0206718.1"
                     /translation="MYDYPYQNPKLTIRQRVEDLLSRMNIAEKVGQVNQHLYGWECYE
                     KNGDKIELTEKLKEHVKWGGGLGALYGLFRSDPWSKTDYKNGIPAKESWKVANLVQEY
                     VLQHSRWKIPVLLVEECPHGHQGLDGISYPTNIGRGNTFNVALLEQSSRLMAKELAAK
                     GVHLALVSTLDLAKDPRWGRTEECFGEDPILSARFSEAVVRGFQGEIISEKVSFLDQT
                     VEEINKKKDQIGVVLKHCIAQGEALGGHNSGTVTIGRREFSDIYGPLLKSTRNAVGVM
                     AAYNDIDGIPCHINRALFEQTLRKEIGYQGIVMADGVALDRLSDVFTDKKTAAAYALA
                     AGIDLSLWDETYTKIAEAIDNQVVDEKLLDQAVRRVLSVKFLLGLFEQPFANDPKEYW
                     DHLMEESEHLNLETAKESITLLKNDGILPLDQQVKNIAVIGPNAHDVYHLLGDYSAPQ
                     TEDRLKKKIVQEIKTEFPQSKVSYAQGCEVRNQEDQEEKLLEAVTLAQNSDVIIAVLG
                     GSSARNFDMEFLRNGAVSSKGINMDSGENVDVASLSLGGYQEQLIEQLYQLGKPIITI
                     LVQGRPYDLMKIESFTNAIATAWFPGQEGGKAIAQMISGRNNPSGRLSLSYPRNSRQL
                     PVYYYQRAASKQENYYDLSGSPFYPFGHGLSYTTFEYSQMEVKTDIDKVIISIKVKNT
                     GSVSGKTSTLVYVRLIDGVVIQRTKLLKAFRKDELRSQEERLLVFELTSEDFSYMDID
                     DKRHYAKKAVIMVEQQEKEIKCNWQE"
     gene            complement(23999..25324)
                     /locus_tag="ACG8PO_001382"
     CDS             complement(23999..25324)
                     /locus_tag="ACG8PO_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFQ0206719.1"
                     /translation="MKVVDRLSEKMLPIVSKMSSQRHLLAIRDSFIVTMPLVMAASVM
                     VLLNALIFSNATVQRILDLSPLAELASIVNNGTMSILAIIVCYNIGWNLATHYIQTGV
                     IDDPSFSPVHAGILSTAVMFILMSLSSTVTLMDGNTAEATGVFLQSLTSSSGLATAMI
                     AALVSTELFIKLAKMKSFKIRMPEGVPPAVAASFNSLVPEIVVILFFSVVVFTLNHTT
                     GLNVPQLIELAIQTPLKAFVLSVPGILFLQFFSDFLWVFGMHGSSILAPIRQAPLLQS
                     IQENMDAFSVGKEIPNIITEPFTNAFGLIGGGGCILPLVIAILWASKRQEQRSIAKFG
                     LTTCLFNITEPIMFGLPVVMNPIYMIPCAIIPSINLVIAYAATSLGIISKTVAAAPWI
                     TPPVIQSFIATGGDIRAAVLTVILIILDVFLFLPFVLAANKAKLAEGGY"
     gene            25555..25980
                     /locus_tag="ACG8PO_001383"
     CDS             25555..25980
                     /locus_tag="ACG8PO_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0206720.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DITNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGADSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKEATVTFTKIGNYRLKDKDGNIYSL"
BASE COUNT         7680 a         5568 c         4593 g         8589 t
ORIGIN      
        1 atgcattttg attgtgaatt taaaagaaag aggttattta tggaagataa aaaattgaaa
       61 aaacaactta gctcccgaca tatcaccatg ctagctttag gtggagcgat tggtgcaggt
      121 ctatttaaag gaagtggcga agcgattgga atcgccggcc cttctgtatt gatcgctttt
      181 ttgattggcg gcataatttt atatatcgtg atgaaaggtc tgggaaaaat tgttttaagt
      241 ggtggtgata cgcaccacgg attatctgga ttgattcgtc catatctagg tgctcatgct
      301 gccgatttta ccgattgggt ctactggtcc atgtggatta tcaatatcat tgctgaagct
      361 gttgcagctg cttccttttt acagttgtgg ttcccccaca tcccagcttg ggtatttgtt
      421 tttatgttgg ctgttttaac tactatcatc aatctttatt cggtacgatt attcgcagag
      481 acagaatatt ggctggcctt tgctaaaatc agtgtcatta tcctattgat catttttgct
      541 gcttatcttg tcggtcagca aatgttgggt actggtgttt tccctaccct tcaacaatta
      601 actgatcatg gtggttttac tccccacggt atgaagggaa tcatcagttc tttgcttgta
      661 gttatctatt cttacggcgg ttctgagttg attgctatca cagtaagcga agcagatgat
      721 cctaaacgtg cgattcctaa agcaatcaaa ggcgtcatgg gaagaatcat ttccttttac
      781 attattcctt tatttttgct tttgattatt tttccttgga atactttagc aggcacgaac
      841 gttagtcctt ttgtgatggt atttgaaaaa atgaatattc catttgcggc agatatcgtc
      901 aatttcgtga ttgttcttgc tctgttctcg tctatcaatt caggtgtata tgcttcttcg
      961 agaattttgt tcttccgctt gaaagatcgg aaaggttcct ctagaaaact agctgtttta
     1021 aacaagcatc aagtaccgca acgtgctgtg ctgttttgta ccagtgttct ttatctaggt
     1081 gtggctcttt cttattttgt cggtgacaaa ttatttggct atctggcagg ttctctttcc
     1141 tatacagtat tattgatctg gatcattatc agtgctgcgt cctttgtact gacactaagg
     1201 aaaggaacgt tatgggacaa aggaatcagc ttttttgcat tagcagtctt atggttgatt
     1261 ttccttggta tcctattcac gaactccatt ggggtaactg tattgaccgg tttgctttac
     1321 ctgttcattt atttcagtta tcaaaaaaag aatgatgctt tcgtcttgac gaatgaatga
     1381 aaccaataga gaattttatt ctatttatat tgaatatctg gagctattga aaaaaaggag
     1441 ctgcgacaaa aattgttgca gttctttttt ccgaataaac ggtgttcaaa aaccatctgt
     1501 gcagattaat tgattgttct ccacgctcat gacatgatag aagaaaggaa tgaagaaaaa
     1561 ggagtttgtt ttaaatgaaa aagtattgga agtttttgtt gatgattggt gtttcgacgg
     1621 tagtcatgtt tggtatgatg tatttgaatg tttatgcgct tgatcatatc ttcttcagtc
     1681 gcacacgtgt attcatggcg ttgatgatgg gtgcagtcat ggcagtcatc atgttgttgt
     1741 tcatgtggaa aatgtatgaa aacaaaaaga taaatgctat gattttagcc gctagtcttc
     1801 ttgtttttgc cggttcctta tttatggtac gaagccaaac aactgtcaac gatacggcgt
     1861 ggatgaaggc gatgatccct catcactcta ttgctatctt gacaagtaaa cgagctgatt
     1921 taaaagatcc tgaagtaaaa aaattagcgg aagaaatcat tcaggcacaa gaagaagaaa
     1981 tcgacaaaat gaaaaaaatg atcaaagaaa tggaatgacc attatccatc caaataaaaa
     2041 aagaggatgt tccagaagtg atcaacttca agaaataaga agaagctact tctggaacac
     2101 cgtgaatcag aggttgtgaa aaagctgctc tgcatcaagt aataagaaca gccaaacaaa
     2161 aatagcttct catatttttc gtttggttag gcttattacc gcaaatgcaa gcttttgaac
     2221 accgtttatt cgtaaaaggg agtgtgacaa aagtcttgtc acactccctt tttacgtttt
     2281 tattgttcta attgcgctgt cttgcttact gcttcttgcg gtttcacaaa tttctttgag
     2341 atcccccaac ctgtaaaggc actgaccatc gaaaacacag gtgaaagcaa actgaaaaac
     2401 acgaatggca agtaagtgag tgtaggaatg ccaaatgtac ttgctacgaa acttccggcg
     2461 attccccaag gcactaagta gttgattact gttccaccat cttctagtgt tctccctaat
     2521 acagttggat caaatcctgc tttttcgtaa gtttctttaa atgcattacc aggcaggata
     2581 acagatagaa attgttctcc aataaagata tttactccga tgcttgtcat caatgttgta
     2641 aaaatcaatt ttggggtgtt cgtcatcttt cttgatactc gttccataac ggttgagatc
     2701 aacccaaatt cgatcaacaa accacctaat gacaatgtta gaataatcaa tgacacagtt
     2761 ggcatcatgc tttcgattcc tccacggcta agtagtgaat cgatttgttg gtttccagta
     2821 tttgaaacaa atcccgattc gatgacccct gccaacttcg ttaaggaagt ttgtgggttt
     2881 tgaataaaga tcattcctac tgctacagca atattcaaca aaatcgtcaa aattgccggt
     2941 acctttttcc atgcacacaa gagcattaaa aatagaggaa tcagtgacca gatcgaaata
     3001 gtaaagtttg cttctaaaat atcagtcact tcacgaatag atgaaagact tgcggcttta
     3061 cttgtgtgtc ctaaaatcat gaataaaaag aaagaaacaa caaatgccgg aacagttgac
     3121 cacatcatat gcttgatgtg tttgaacaaa tcggcatcaa caatagctgc tgctaaattt
     3181 gttgattcgg ataatggaga catcttgtct ccaaaaacgg cacctgaaac aatggctcca
     3241 acaacaaggg ctggatttag tcctagtgtt gtcccaatac cgaaaaatgc aatcccaatc
     3301 gttgacataa cagtaaaggc actccctaca gcgctaccta cgattgcaca aacaacaaat
     3361 acagaaggga cgaaccactg gacactaatc aatttaaatc caatgaccat gagggttgga
     3421 ataattcctg cttgtatcca tacagcaatc aatgaaccga ctaaaataaa aataaagatt
     3481 ggaatgatcc ccggtttgat gccattgatg atcccttcgt gcagccaatc tgtcgggtat
     3541 ttttttatca ctgcgtacaa catgaccatt ccgatagctg ccaaaatcgt gatattaggt
     3601 gaaaccttta atccgacaac gccaatcgca atgcctaaaa caatgaccat caatacaagt
     3661 gttgcttcta aaaaacttgg tgttcctttt ttttccatga taatctctcc ttttttagtt
     3721 gttcttatgt tctaaaaaag ttaagatatc ggattactcc gccacaattc atccgcgatt
     3781 cctcctttat aaaaataaaa agacctgctt tcaactaagg acgaatgact ccgcggtacc
     3841 accttaatta aaaacagatc tgtttttcac tttcaaacga ttattcaaat gtatggactt
     3901 tccatgtaat tcgtcaatta ggcttgatcg gttcacagcg accaccgact ttctgaacaa
     3961 aacacctaac cactactgcg tgaaaagttg acttgtatga agtttacctt ccataagatc
     4021 atttgtcaat gaaaaagaaa atattttttc atttttctga aaattatttt tataactttc
     4081 gcgcacaact ttatcttttt actagaaagt agcgccctct tgtttttttc ttgctactat
     4141 ttcttgatgt tcttgtttga ttttttctaa tagcttgggt tctctcaata aatctagagc
     4201 tgtattggca agtaattccg ctgcaatcgc aatagaagca agtccttttt cacttttagc
     4261 agcagctttg aattcttctg tatgtccggc aatatatgtc tcagagattg ctacagtagg
     4321 ctgtatcgtt ggaataactt ggctaacatt tcctacatca gaagaaccta gacttgttct
     4381 ttgttccgtt tctatttctt catcaggaac ccctgcttct tttgcatgtt ggaagaataa
     4441 gtcatcgaaa gaaggcgtta cgatgacatc atcgactgca ttttggaaca atccaaactc
     4501 aaaagtcgta cccgtgctta atgcagcacc tcgtacgata ttttccactt tttcatatac
     4561 tttgtctaat gtttgtcgat ttcctgcacg taaatagaaa cgtccagcag cgtagtctgg
     4621 tatgacattg gccgccacgc ctccatctgt aatgattcca tggatattga catcttttgg
     4681 caattgcaag cgtagagcgt tgattccgtt aaacacttgg atcaaggctt ccaaagcatt
     4741 tactcctttt tcaggtgctg ctgctgcatg agaagcgaca ccgtaaaatt tgatatcgac
     4801 tggatcattt gctaaagatt ctgtcgtttt cccataacga aaagctggat ggacacataa
     4861 tgctgcatcg acatcctcaa aaaaaccttc ccgaacgaag cttcccttag ctgatccatt
     4921 ttcgcctcct tcttcccccg gagtaccata cacacgaatt tctccgccaa tctcatcaat
     4981 gaattgtttc actatgctgg ctgctaatac ggagtaagta ccaaataaat tatgtccgca
     5041 tgcatgacca atacctggta aagcatcaaa ttctgccaaa aaggcaattg tcggacctgg
     5101 tttattggaa atataacgtg catcaaagcc tgttcggtgc ccagctactt ctttttctac
     5161 ccagaaccct tctttttcca attgtttgat taaagctgct gaggaataaa attcataatt
     5221 acttacttca ggatggtcgt ggatagctag tgcaagtgct tgatattctg gtaaacggta
     5281 ttgaatcaga tccttcaatt gatttgttga gagtgtggtt gtcataaaaa tttcctccta
     5341 tgctagtcgt tttttcttat tttctcccga atgtttccca cgcaggtacg cttgaacctt
     5401 ttgatgtttt ttcgattact ttggctgttt cttctgtttg ataggcatct actatttttt
     5461 gatacgtttt attgtcttca tcttcttttc ttgcaacgat gatattgaca taaggacgtg
     5521 catgctcatc gactggttct aagaagattg catcagaagc gggattaaat ccagcatcta
     5581 cagctacacc gctattgatg actgaaatat ctacatctga caaagcacgt gctgtttggg
     5641 aagcatctaa ctcagaaatg tctagatttt ttttattctc ggtaatatca gaaacggtag
     5701 gcgcttggcc ttttgccttg tccactttga tcaatccagc agtttgcaat aacagaagag
     5761 ctctgccgcc attcgtgaca tcattaggga ttgcaacttt tgctccatct ttgatatcag
     5821 ccacatcttt taacgtattg gaataaattc ccaacggtgc attaaccgta ttcccaatcg
     5881 atactaaatc agttccgtgt tcttcattgt aattgtctaa aaagaattga tgttggaagg
     5941 aattcaaatc gatcgatcca tctgctaaag ccgcatttgg ctgattgtaa tctgtaaatt
     6001 tcacatattc caaatggatg ttttcctttt tcaaacggtc tttcacatcg tcccatacat
     6061 ctgtatcttc cccaatgatc cctagtttta cggtcgtttc ttttttttca gttgtagctg
     6121 cttttccttg gctgcatcct gccaaccaaa taattgctac aaatgtaatg actgcaaaac
     6181 gaataatttt tttcattttt tctcctccat tttcaatgtg tgactttttt aatcagttga
     6241 ttactgataa attgactcaa aaaaactaag accagaatca ataatgtagc gacaatcgtt
     6301 acatctgttt ggaatcgatt atatcctctc gatatagcaa gattccctaa tcctccggca
     6361 cctactgctc cagccatggt agtcagtccg atcacattga tgatcgtcaa cgctgagacc
     6421 cgaatgatac caggtaaccc ttctaacaaa taaacacgta aaatgatgcc gattgggctc
     6481 gtgcccatcg cttctgctgc ttcgatcacc ccaggatcaa cttctagcag tgcattctcg
     6541 atttgacggg caaaaaacgg gacaacgcca aaaatcaaag ggactaaagc tgctttggca
     6601 ccaatcgtcg ttccaactaa gaaacgagta gtcaaagcta aaagagaaag caagataatg
     6661 aaagggatgg aacgaatgat attgatcaac ttatcaagta tttcgaacag cagcctgtta
     6721 gctagtaatc catttggacg tgtcactact aacgtcacac ctaagatgat tccgatgatc
     6781 ccaccaatga atgccgtcca aaaagacata tatagtgttt gctttgtcgc ttcgatgaat
     6841 tcatccggaa tcaaatagac attcggcaaa tatgttttaa aaaattcagt cattttagtt
     6901 tcctccttcg atgactttga tacggttatc taattcattt ttttgtaaaa tagttacttt
     6961 tacaccagat ttaatcaaat aatccaatgc tttttgccgc tgtttggttt ctccagctaa
     7021 agtaacgatc aaacttccta agggtgtttc ttgtaatagc tctacattac cgtataagat
     7081 attcgctgtt acttggtact tgctgtaaag atgagcgatt agaggttcgt tcgtctggtt
     7141 gccgatataa cttaattcca ccaaccattt atttgcaatc tgatcggcaa aagcagcatg
     7201 ggcactgatt gtttccaatg cttggtcaag gtgtgtcgct gtccgaataa aatctttagt
     7261 caattcttct tcaggatgac tgaaaatctg gatgctgcta cctttttcaa tgattttccc
     7321 cgcttccata accgctacct tgttgcagat ttctttgaca acttgcatct catgagtgat
     7381 caggacgatt gttagaccta gctgacgatt tagttttttt aacagtgcaa ggatctgaag
     7441 ggtcgttttc ggatcaagtg cactagttgc ttcatcgcat aacaacactt taggttcatt
     7501 tgccagagct cgtgcgattg ccactcgttg tttttgcccc cccgacagtt gtttaggata
     7561 cgcagatgct ttttcttcca gaccgacaag ttccagtagt tcattcactt tttgacgacg
     7621 ttcctgtttt gattttccag aatactttag agaaaaatct acgttgtcaa aaatcgttcg
     7681 actttccatt agattgaaat gctgaaagat cataccgatg gcctttcttt cttttcgcaa
     7741 ttcttttgga gagagctggc ttaaattcgt ctgattgacg atgacttctc ctgctgttgg
     7801 tttctgcaac aaattcatca cgcggactaa cgtactcttt cctgcacctg agtaaccgac
     7861 gatcccataa acatcccctt tttcaatcgt taaatctaca gaatctactg cctgtatctc
     7921 tttttctttt tgtttgaaac atacagaaat atctcgcaat tcaatcatta acattcctcc
     7981 ttgtctataa aaaaagcatc ctcaagaaaa tgaaaatagt cattttcttg aggacgcctt
     8041 tttgcgtgtt accacctcat gttcaagctg tgttcacaca caactcctct tcaagtacca
     8101 tcatactcta gcaagatatc ggtcgcagcc gtcaccgcct tacatctatg ctcgggatga
     8161 attgctcgat ggccattttc caatagtcgt tccttgcttc ttctcagcta ccgaagctct
     8221 ctgtcaaagg ttcttctatc gtacttacca tttcaatgca aataatattc aaaaaaaaaa
     8281 tccttttact aacataaact atgtcagtaa aaggacgacg tatcgtggta ccaccttttt
     8341 tcggaagttt ccttccctca agcccaacat ctcaaaaaga gagattggcg ttcgttttaa
     8401 cggtcgaaca tttccgaatt tgcctactta tcgacaaatt attctccaag gccattttca
     8461 gcttttctta cttgactctt cacaccagcc agagcctctc tgcaaagttt ccaagcttac
     8521 tttccttctc aacgaaatca atattttgtt gttgggaata atctacagga ggaaaactga
     8581 aaagtcaagc atgaaatcca aattattttt tctatattta tttttctgtt tttttatcta
     8641 acatttcaat aacagttcga agatcttttc cttccgcaag aaggttcaat ttttccttct
     8701 cttcgacgct ataatttcga acaaaactgg aatcgggaat caaaacaacg cctagtcctg
     8761 cacgtgaagc ggctgtcgct cctactaaag aatcttccac tgcaaaagct gaaagagctt
     8821 ctaatcctgt tcgttccaaa gctgtcagat aagggactgg tgaaggcttg ttctccgata
     8881 cttcatctcc gaacgtaaac gtatcaaagt aatcggctaa ttgaaagtga tccaagatat
     8941 ctgttgctct tttcgtaaca gttgtcgtag caatccctag tttcatatga cgttctttta
     9001 agtctgtcaa tatttctttt gcatagggtt tcaattggat agctccactt gtcagctgtt
     9061 gataaaaata ttcttcacgc tttgctcgga cttcctgcac tttttcagga ctgcctgctg
     9121 cctgcactaa aatatcaaaa ctttgtttcc agttttttcc gccccaagcg tcaacgattt
     9181 ctttcgggat atgaatatca aattgctgga atccccaaag ccaaccatcc cgatacgttc
     9241 tttccgtatc cactaacaaa ccatctaaat caaatattgc acctttcatc tatacattaa
     9301 tcctttccaa aacaatacac ctagtttagc atgactgatc caaaatgaac agaagcatag
     9361 agcccagccg catcacttat aagagttctt ggaatcgacg gatatagatt ttcttcacag
     9421 ctgtcacaag cgccagataa gcgacgactg ttataaacaa ccataaccag taattgccag
     9481 ggagcggcat caagcctaac tcagcaccaa atcctgtgaa tggaaggact gaaccgacaa
     9541 tgattcctaa tgtagtgata accagcatgg caaatgttgc attgctttgg ataaatggta
     9601 ttttcggtgt acgcaatgca tggagtacta atgtttgtgt ccacagggat tcgacaaacc
     9661 atcctgcatg gaataatgcg ataaaaatca cttgttgtga gctatccaat gtatggaaac
     9721 ttccaccaac tacagcagga caaataacaa aatacatcaa caagtaagta gtgatatcaa
     9781 aaatcgaact tgttggacca aaataaacca tgaattttcc aatactagat gcattccatt
     9841 tctttggctc atccaaatat tctttatcca tccgatccca tggtaaggaa atgcaagaaa
     9901 cgtcatagat cagattcaaa aatagaagtt gcaaaggcaa cattggtaaa aatggtaaga
     9961 acgtactggc aatcagtacg gaaaacatat tgccaaaatt agaacttgct gttgccttga
    10021 tatatttcat gatgtttcca aaagtttctc ttccagaaag aaggcctctt tccaaaatcg
    10081 tcaaatcttt ttctaaaagg atcacgtctg ctgattcttt tgcgatatcc acagctgtat
    10141 ccacggaaat accgatatca gccactttca ttgctggagc atcattgata ccatctccta
    10201 aaaatccgac agtatgccca tttttacgta aagctgtcgt caatcgtgct ttttgctgag
    10261 gattcaattt tacgaatact tcgtgttctt cagctacttg cgctaattca tgatcattca
    10321 tttccgagat tttctcacca gtaatcaatt cattgatttc taggcctact tgacggcaca
    10381 cagaccgtgt gactaattca ttgtccccag tcagaacttt tacagctaca ccatgttctt
    10441 ttaaagcttt caacgcttgt tttgttgttt cttttggtgg atcaaggaaa gcaagataac
    10501 caatcagaac catgtctgac tcatctttga ctgaaaattc tccaacaacg cttggattgg
    10561 tcttttgtgc gacaccaatc actcgtaagc cgtcttcatt aagtgattgt acttttgcaa
    10621 gcaccgcttc ttttcgttct tttgttaatg gtgcgacagt accaccaaga tcaataaatt
    10681 tagaaatgtt caacatttct tcaatcgcgc cttttgtgat catctgtgtt ttgccagccg
    10741 gatcagcgac tacaacactc atacggcgac gttcaaaatc aaaaggaatt tcatctactt
    10801 tttgataatt gattgtatct gtatctaaag tttctgctgc ttcatcaatg atagccacat
    10861 ctaacaaatt tttcagccct gtttgatagt agctgtttag ataagcatgg cgcaaaacac
    10921 gatcatcttc atttccttca caatccatat gatattcaag aatgattttg tcttgtgtca
    10981 atgttcccgt tttatccgta cataacacat cgatcgcacc aaaattctgg atagcattta
    11041 aatttttgat gactgttcct tttttggcca tcacacttgc tcctttgacc aaattcgtcg
    11101 taacgatcat tggaagcatt tcaggcgtta aaccaactgc cacagataat ccaaaaagaa
    11161 aagcttctaa ccaatctcct tttgtaagcc cattgataac gataactgct ggagccatca
    11221 atgacataaa tttgatcagc agcatggatg ttttgtggat gcctaattca aaacttgatt
    11281 ggatgggttt ttcagatagc gtttctgcta catgcccaaa tagtgtgtca tttccgacag
    11341 aaacaatgat tccttccgcg cttccactga tgacattgct tcccataaag acaagatttt
    11401 cataacttgt ttcactttgc tgatcgtctt cacatacttt cgcatgcttt tctactggat
    11461 agctttcccc tgtcaatgct gcttgagaaa tgaatagatc ctttgcggac aataaacgca
    11521 tatccgctgg tatcatatct cctgctgaaa gccgaacaag atctccacag acgatttctt
    11581 ccataggaat ctctgtatac tcgccatttc gtttgacagc tgccgttact ttcacaagtg
    11641 atttcagttt ttcagctgct tgattagaac gcacagactg gatcaacgtc atcgtcccac
    11701 tgacgataac catagtgaag ataatggcta caccaaagag atctttatct cctggagcgg
    11761 ctaatacgta ttccgtaatg aatgaaataa ttcctaatgc aatcaacact aatgtaaagg
    11821 gtgtgatgta tgctttcaat atttctataa tgaatggtgt ttttttgcca tatgaaatcg
    11881 cattttcacc ataagtttct cttgaacgct cgatttgttc ttctgacaat ccatttggtg
    11941 atgcatggaa caattcaaat aattcttgct tcgtttttcc agcaaaagct gcatagattt
    12001 tttgtgtatc tttctttttt tccatctcga tctctccttt gtgtccaaaa gacagctgtt
    12061 ctcgctgttt ttggtctttt gctattcatc ggatcgatag agaaaatgct gaaaggggct
    12121 gagaacagat agataatagc cctggccttt cctctaaatc cttgtttttc gaatgactgt
    12181 catccatttg ttctcatctc ctttactaag taattttaat tcatgacttc caatcatttt
    12241 tcgaatagtt attttcccaa ctcgctcacc tccctttttg ttttttcacc tatgggattt
    12301 gcggcaacaa aaaaacctct cgccaaatac tcagcgaaag gtttgaaaaa gcaaatcaaa
    12361 aaaagcttct cagatctcat cgttgagttt tgacactgta caacgtaaaa agcagatatc
    12421 tgcttttagc taccttatga aaagccttat ctcgacaatt cctgttctac ccattggcgt
    12481 ctctcgacgt ttttgggcag tagcctatgt ttgtatagga gcctcaccta acaaatccta
    12541 ttcggttttc cctcgttaat ataacaacta aaaaagagga tgtcaaatca attttccatg
    12601 tttttcatac ttttttaaaa tacacgcgtt atatttcctg tctcacgcag ttttatttgt
    12661 ctcattttcc acctcccgct ataatagaat tatagtagga aacgaggtgc tgttatggaa
    12721 cgaacacttg ttattatcaa gccagacggc gtaagacgcc atttggtcgg aagcatcatc
    12781 caacgttttg aagcaaaggg actggccatt gctgaaatga aatttgcaac gatgacacct
    12841 gaactagcaa aagaacatta tcagcacttg actgaacgta gtttttttga tgagcttatc
    12901 gattacatga cttctggtcc ggtcgtttat cttgtgctgg tcggcgagga agtgatcgat
    12961 atcgtccgta agatggtcgg agcaacaaaa ggggcggatg ccgttccagg tacgatccga
    13021 ggagattatg cactgccggg gactgaaaac atcatccatg cttctgattc ccgtgatgct
    13081 gcaacaaaag agatcgcccg ctttttcccg ccattcgata caaacaaacc gtctgaaaaa
    13141 gcggcgaagt gatacaaatc ctaaactgtt ggtctaactg ctgggtttag caatggccag
    13201 cagttttttc tctaaagaat tcctttgatt cttccctaat gtatattcga tcactgcgaa
    13261 aaactcttgg tgattttccg atttttcttc ccctaaagca gcagaaatca gtcgttggta
    13321 ataatccgcc atcaatgttt cataaatcac tgtacttctt tctttatgcg ctaaactccg
    13381 tttcatatct tcattcacgc tacgtgcctt ttttaagcga ccattttcta tataaaacaa
    13441 agtcaagttc gtcaaaaaag taaagagata ctgctcgtga ttattcagtt gcgcgatccg
    13501 tcgcattctt tttttgattt tcaaataatt attctcaata aaacgatcac tgaatatcca
    13561 aggaaaatct gctagaatag atacttcaaa ttgcccatac tctggtactt gcatcaaata
    13621 ttcttcaatg atacgacatt cataacgcaa aaattcaaat tccgtctcag atacttctgt
    13681 ttttttggac atgtgcagat tcaaaaccaa atagtacaga tcttctgttt tcttatagtg
    13741 cctgcggtta ttagcaattt ctttgtaagg atcaaagctg tcttcttgaa tccgatctag
    13801 cattcgtctt tttttcttta atttttttgg ctcatcaagc tgatttcgta tccattcata
    13861 ttcggcaagt gtcatgttca ttcgctctaa aaatgatttc aatgttccaa acgatacttt
    13921 tttcactgaa tttgtctcga tcccgactag cgaagtttta gaacagattc cttccgctaa
    13981 ttgggtctgt gtcagctttt tgttgactcg aagcttctta atcaattctc catcatacat
    14041 aatagttctc cttttttaat tgaaattaac ccttggcttt tccagaatgc ttgccaataa
    14101 gaggaatgat tatttcaatt taaattttcc gcattcaata aatagatacg ttaaaaataa
    14161 aaaatacacg aaaaaaataa aaaaatgttt ttctcataaa aaataccaac cttcaagcat
    14221 gagactgctg tctaatccct gaaggttggt ttgattgttt attgatcatt tttttactgc
    14281 tccgcattgg ttcctgttac tggatatagg atagtcgttg ttttattgcc gttttttgaa
    14341 aagacactgg tcgaatgttc acctttttgc ttttcgattg cttcattacg ttccgcttcg
    14401 tttttatagt catatttttt cggatcaatc gtctcaaatc cgtttggcac ataaaaccgc
    14461 aagagatttt cctgattcag cttatctgaa agacttaacg cagtcttgac tttttcctga
    14521 tctttggaga tttcctcgtc gattcctgct gctttcgtat cgatgatttc tcctgtctga
    14581 ttattgtaag cttttcctcc taggacggtg tatttgggcg tcacgaagtt cccatttcga
    14641 aaagcaacta cttgatcgtg ctgagaagat aataagtctg taccaaaatg aagataatct
    14701 ttatcatcga ttcccaatag atgtagtaat gtcggcaaaa catcgatttc tccaccatat
    14761 tcatgattga ctgtcccttt agtgtaacca ggcatatgga tcatcaatgg cactcgttgc
    14821 atctgtgcgt tgtcaaattc atcccatgta tctggatctt taccaagagc acttgctaaa
    14881 tctttattat ctgtattcga gatcccaaaa tgatccccgt aaatcacaaa gattgaattt
    14941 tggtaaatac cagatttttt caaatacgta aaaaattgtt ccaatgattg atccagataa
    15001 tgggccgttc gaacataatt atctactgtg ctattgccag tattcaaaga tggaaagtcg
    15061 aaattcttgt catccgtata ataaggcgtg tggttggtta cggaaagaaa tttcgtgtaa
    15121 aacggctgtt gtagatgttc aagatattgg gcagattctc tgaataaatc tttatctaat
    15181 atcccataac ctaacgtttc atcggattca tcaaaataag atcgatcaaa gaaattgtca
    15241 taccctaaat ttttatatac atgatctcta ttccagaaac tagctacatt cccatggaaa
    15301 acagcacttg tatagccttc tgtttgacca agggtcgctg gtgctgcttg aaagacatta
    15361 tctgacccaa gttctgtgaa taatgagcct tgaggtaaac caaatgtgcc agtttccaac
    15421 atgttttcag catcacttgt ctttccctgt cctacttcat gaaagaaatt gtcaaaactg
    15481 atcgttgctt gattctggaa aatactattt aaaaacggcg tgacttcttg tccgtctacc
    15541 ttcatattga tcaaaaactg ttgaaaactt tctaaatgaa ggacaatcac attttttcct
    15601 ttagcaatcc caaatgtttc aggatttggc tctgcataat gtttctttgt atagtctaaa
    15661 acttcatcaa ttccattact gcttgcatgc gcgcgaacac tgctagtcat tcctgttttg
    15721 attccgtcat aaatagtgta cgcatcgatt cctagatatt tgacaatata ggaacggtca
    15781 aatgtccgct gtagcaattg gggacggttg gcttcgctca acgctaaatt tcctgaaaaa
    15841 atcaatcctg aaagacaggt aacagcgata gccatcggtt tgctgacagg gttgcttttg
    15901 ataggtattt tcttgacctt caaccaaacc agtagcccaa tgaacacagc gatatccaac
    15961 caatagatga catcatgggg tttcatcaaa gaaaaggagc ttcctgacaa cccttgtgac
    16021 actttcgaaa agcctaatac agatttgact gtaatgaaat ctgtgaattc ccgataaaaa
    16081 ataatattca agtacaacaa aagcgtattt aaaatatcaa tgatcaaaag aatggctaac
    16141 gctggctttg gctttttaat atacaaagaa aggccaaaaa acaataaggt agtcgcaatc
    16201 gggttgatcc acaagataaa gtactggatc gtaccttcca cacctaatga aaaatctgca
    16261 tagtaggcaa tgatcgtctt cagccagaaa aagaaaacag ctgtcaaaac gattgctaaa
    16321 cgattttggc acaatttttt aagttgtgtc acgtatatca cttctctcta ttcacatttt
    16381 ttcttatttt gctcctgaaa aatgaaaatt tcctaaaaaa gttgttatct taagattttt
    16441 ttattctttc taaagattat tccattcatt tttcacagca taaaatcgtt tcggtgtaag
    16501 cggttaaaat agtaaggaga taaaaatctt gtggtgaccg atttactcta tagtattcaa
    16561 aagtagtttc tcggtattcg tacaaaaaaa acatactaag attagagggg aacatcattg
    16621 acgaatgatg ttctcctcta ttttctattt ttagattata gtgaagagtg aaagaaagtt
    16681 cgaacaaagc cttgaggact acgagtccct atcaaaaacc gaacttcttt actgtcattt
    16741 tagaatcagg tttacgtatg ttggggccat gagcccgtgt ataggaaatt ggaatcgaaa
    16801 tgaagcagta aagttctttc aacacttaaa aaggaggaaa acaaatgcct tacattcttg
    16861 cattagatgt ttcaatggga cacagctaca gtgtcctata caaagacgac acctgcttat
    16921 ttgaagacga gatcgaacac actcaaagag gatttcatgc attactcgaa gagatccagc
    16981 acttgcctga acgtccattg attgtttttg aagcaacggg tatttattcc agaccagtcg
    17041 aaaagttctt tcaagatcat cacttttctt actgtttgtt aaacccgtta gaagcgaaaa
    17101 aacagatgga agaaaacacc ttacgcagtt ggaaaacaga taaatcggat gcccatcggc
    17161 tcgcacagac tcatcttaaa aatcaacgtc aaccaaaaga agtacaaagc aatttttact
    17221 tagagatgcg cgatttagcc cgtttttatc aagaaattga gaagaaaatc actcgactgc
    17281 gcatggattt acacaactgc cttcagttga cttttccaga gcttgaacag ttcttttcaa
    17341 acaggctgac cccttacgca ttgacattga tccgtctttt tccccatcca gattttgtat
    17401 tggcatctac tcggacaaaa ataaaaaata aactgatcaa cgaaacacgg gaaaaaatct
    17461 ccgcgaatcg tgccgagcaa aaggcagatc aaatcatcca ctatgcacag tgtgcctatc
    17521 cagctgttga aaaagacagt attcactgtc aaaaaacgat ctattacgcc gaacttctcc
    17581 aagatttact cgaacaaaaa aaagcactgg cgacacaaat gatcaaaaaa gccgaagcct
    17641 ctccttgctt tttgttgtac caaacgttcc ctgggattgg cgcactcaca gccgctttat
    17701 tgctcggtga attaggagat atcacacgat ttaaaaccca taaacaattg aatgcctttg
    17761 tcggaatcga catccgacgg tatcattcag gtaagtatac tggccaagac cgcatcaata
    17821 agcgtggtaa tcccaaagca cggaaaatta ttttcttcac cattcgaaat atgattcgcc
    17881 agcaacgcgc agctcctaat catattgtcg attattacta caaattaaaa aagcaaccta
    17941 tccctaagaa agataaggtt gccacagttg cttgtatgaa caagcttctg aaatgtatgc
    18001 acgctatggt tagggcacat acagagtacg actatgcgta cgcggtctca gtggaccatt
    18061 aactaaacca cttacagtat accacttctt tgcccagatc gaaaattttt tacgaacggg
    18121 gctttattta gtgtgccttg attttgtcgc tttcgtttac gagctatttt tcttgcacct
    18181 tttacttgac taatcgtagg aaagaggctg tgacaaaatt cttgtcgcag ctccctattt
    18241 cttgatgcag gacagctttt tcacaacctc tccactaaac ggtgttcaat cagctgacct
    18301 tcggtattag ctcaaaaaac ggcaaaatat gaggagctat tttcccgttt ttttctctaa
    18361 tactcaggtc aaaccgctga tttcacaacc tctgattcac ggtgttccag aagtagcttc
    18421 ttcggaaata agccgaaatt ttacaaaaac ttgagaagca cttttcgtaa atttcttctt
    18481 atttcttgaa gctgaccact tctgtcacaa cttctgtttt tcattgtcca taggtttgat
    18541 attgtccttc tgatgactca ttcgtaatga ttgaggcttg attttcttca tttcccgtta
    18601 tcgtttgtcc actgccagaa tctgaagggc ttagttctgt cgactcgttt ttttgagaag
    18661 aagcagttgt tttcttgact tgttcgttgg tctttttcca ttccgctagt cgatcggcca
    18721 ccactttttt gtccactttt tcagaataag ctgtaaccac ttttccattt ttcaaacgtg
    18781 ctaccttgtc gccatcatga gtcaaaagca aaacgaccaa atgtttgtct ttgaattgat
    18841 attgtctgag agaaatcgca ctaagtacgt catcgatatt gataaattga ttttcttctt
    18901 ttccttttgg caaatagatg tccaattcac ctttttcgta attggcagtt aatttatatt
    18961 cctcccacca ataagttgag aattcttttt tgaaattatc tactgattct ttgcttgcta
    19021 cgattttttc tgtttctttc tgagcaagat ctgctgtcat ttttcctgaa aatgctgcgg
    19081 ataaaataag cagtaagaaa aaaccgaata accaaaagag aagaaacggt ctttttttcc
    19141 tcatgccttc ctccactcct atttaatttt gactattcta ttatatacaa aaaatgaaat
    19201 agaaagacaa aaaagttgaa aaagatcgta aataattgaa cttctttttc ctgaagcggt
    19261 aagaacggga attttcatac attctctctt ggctattctt taagcttcaa atccatttct
    19321 gtctgataca tctttgaacc aatgacccga tttcttgatc gttctttttc catcttgtgc
    19381 taaatctact gaaataaaac cgtaacgatt cttataggca ttggtccacg accagttatc
    19441 catgcatgtc cacatatgat aacctaaaca attggcacct tcttgaatgg cttgatggac
    19501 atatttcaga tgagattgaa caaattcgat ccgataatca tcgtggatca taccatcttc
    19561 cttgataaat cgtgcttcat tttctacacc catgccgttt tcagaaataa aacagtcgat
    19621 attcccataa tgatctcgta cattgatcaa aatatcatag attccttttt catagatctc
    19681 ccatccgcga taaggattca ttttacgacc aggcatttca taattgtcga agtaatcctc
    19741 aggcatagga ccgtttttag tatcgattgg tgtttctttt gcttttattc ggcgtggttg
    19801 gtaataatta attcccaaaa gatctactgt gttttcttta atgatcgtca aatcatctgg
    19861 atgaatctcc ggcattagat cgagttcttt tattctctca atcaattcac tgggaaactc
    19921 gccttttaca gctggatcaa ggaatgaacg attgaagaac gcatctgcta ttcgtgctgc
    19981 tttaacatct tcgggatttg attcgtctct tggataactc ggtgtcaaat ttaagatgat
    20041 tccgatcaca ccgctttgat ccatttcatg gtaagctttg atggctaatg ctgaagccaa
    20101 cgcttcatga taaccaactt ggattgcctt tttcatatct gattcttccg gataatgcca
    20161 tcgatacaaa tagcctgcct cgactggaac gattggttca ttatgggtaa accatttttt
    20221 tactttcgag ccaaacaatt gaaaacaact ttttgcgtat ttttcatagg cttcaaccgt
    20281 ttctcggttc aaccatccgc ctttttcttg cagatacatc ggcatatcga aatgatacag
    20341 attaacaaag ggttcgatac cgttttcaat caactcatca aaatagtgat gatagaaacg
    20401 aacggcttct tcgttgattg ttccagattc atcaggtatc aaacggctcc attggataga
    20461 ggttcgaaat gaattatgcc cagtctgttt catcaactgt acatcctctt gatacttcgt
    20521 ataaacttgc gaggtttttt caggtcctac ttgatcaaag aacttttgcg gttcgttttt
    20581 gtaccaataa tcaaaaatat tctctccttt tccatcgcct gaaattcttc cttctgtttg
    20641 tggaccactt gctgcagagc cccaccaaaa attttttgga aattgatatc ttttcatcaa
    20701 agcacctctt tttctttttt agttgattcc atgtatgata ttttccatca caatcacttt
    20761 ttcattttct tcctcttctt ttttgattcg tttcaacagc tgttctgctg ctcgttcccc
    20821 gattttatct gttggtggcg tgatcgtgat ttttggaagg atactcagat cgtttttatc
    20881 taaataatca tagtgactga acgcaatatc cttcccgatt ctgacacctt cttgattgaa
    20941 acattcgatg gcaccaatcg acatgttgta attgctgaca aaaatagcag ttgccccttt
    21001 ttctaataat gcttttgccc cgtaatagcc gctttttcta gaatagtctc ctgtaaagat
    21061 ctctaaatgg atagacggat ccttttgtac cgcttctttg gctccagcta aacgttctct
    21121 tgctacgtaa tctgtttgag cggcagcaat aaatcctatt gttttatgtc cttgtccgcc
    21181 atgatgagca atcacttttg ctacactttc tcgatcattc actactattg tatcaatacc
    21241 tgctggccca cccggacaat tcaatgcgac aactgggata ctcatttttg tcagcatttc
    21301 aaatccagac cactcttctt ctgataagaa gacgatcaat ccatcgatcg catgcttcat
    21361 caggtattcg attttttgtt cgattttttc tggctgatcg gcaaacccgc ttaataataa
    21421 gctataccca ttttcttcag ttactctttc gatccctgaa acaacctccg aaccaaatcg
    21481 gttagatatc ccgtttatca acagtccaat agagaaactt cgattattct tcaatccttt
    21541 agcaatgatg ttttctttat agtccaacgc tgaaatcgct tctgatattt tttccatatt
    21601 cttttccttc agctgctgac cgtttaaata gcgggaaact gttcccactg aaacaccagc
    21661 catcttagcc acgtcacgga tcgatgccat acattttcac tcctgccagt tacatttgat
    21721 ttctttttct tgttgttcga ccatgatgac cgctttcttt gcataatgcc gcttgtcatc
    21781 aatatccata taactaaagt cttcagaagt gagttcaaaa acaagcagtc tttcctcttg
    21841 agaccgcaat tcatctttac gaaaagcttt caataatttc gttctttgga tgacaacgcc
    21901 gtcaattaat cggacataga caagagtact tgttttacct gaaacacttc ctgtattttt
    21961 aactttaatt gatattatta ctttgtcaat atccgttttg acttccatct gactgtattc
    22021 aaatgttgta tagcttagac catgtccaaa aggataaaat ggacttccgc ttaaatcata
    22081 ataattctct tgtttagaag cagcacgctg atagtaataa acaggtagtt gccgactatt
    22141 tctaggataa gacaaactca atcgtccgct tgggttattt cttccagaaa tcatctgcgc
    22201 aatggctttt ccaccctcct gtccaggaaa ccatgctgtt gcaatggcat tcgtaaatga
    22261 ctcgattttc atcaaatcgt agggtcttcc ttgcacaagt atcgtgatga ttggcttgcc
    22321 tagttgatac aattgttcaa tcagctgttc ttggtatcca cctaaagaaa gagaagcaac
    22381 atctacattt tctccagaat ccatgttgat ccctttcgat gaaactgccc cgttacgaag
    22441 aaattccata tcaaagttcc gcgcacttga tccacctaga actgcaatga tcacatctga
    22501 gttttgagca agggtcaccg cttctaataa tttttcttcc tgatcctcct gatttcttac
    22561 ttcacatcct tgtgcataag aaacctttga ttggggaaac tctgttttta tctcttgtac
    22621 tatctttttt ttcaaacggt cttctgtttg tggtgcagag tagtctccca acaagtggta
    22681 aacatcgtga gcatttggac ctataacagc aatgtttttt acttgttgat ccaacggcaa
    22741 tataccgtca ttttttagta aagtgatact ttcttttgct gtctctaaat ttaagtgctc
    22801 tgattcctcc atgagatgat cccaatactc ctttggatcg tttgcaaatg gctgttcaaa
    22861 taaacctaat aaaaatttga cagataaaac tcggcgaact gcttgatcta gtagcttttc
    22921 atctacgact tgattatcaa tggcttctgc aatcttggta taggtctcat cccacaaact
    22981 gagatcgatt cctgccgcta atgcatatgc tgcagcagtc tttttatctg taaaaacatc
    23041 ggataaacga tccaaagcca caccgtctgc cataacgatt ccttgataac cgatctcttt
    23101 tcttaatgtt tgctcaaaaa gcgctcgatt gatatgacaa ggtatcccgt cgatgtcatt
    23161 ataagcagcc atgacaccta ctgcatttct tgtactcttc aataatggtc cgtaaatatc
    23221 ggagaactct ctgcgtccaa tcgttactgt gcctgaatta tgaccgccta aagcttctcc
    23281 ttgtgcgata cagtgcttta acacaacacc gatctgatcc ttcttcttat tgatttcctc
    23341 tactgtctga tcaagaaaag aaactttttc tgaaataatc tctccttgga accctcgaac
    23401 gaccgcttca gaaaaacgag ctgagagaat cggatcttcc ccaaagcact cttctgtcct
    23461 tccccaacga ggatcttttg ccagatccaa agtcgacacc agcgctaaat gaacaccttt
    23521 agcagccagt tcttttgcca tcaatcgact cgattgttct aataatgcta cattgaaagt
    23581 atttccacgg ccgatattcg ttggataaga aatcccatcc aagccttgat gaccatgtgg
    23641 acattcttct acaagcaaaa ctggaatttt ccatcgcgaa tgctgtagta catattcttg
    23701 gactaggtta gccaccttcc agctttcttt tgcaggaatc ccattcttat aatctgtttt
    23761 agaccacgga tcagagcgaa acagaccgta taaggcaccc agaccgcctc cccatttaac
    23821 atgttctttt aacttttctg ttaattcgat cttgtctccg tttttttcat aacattccca
    23881 accatacaaa tgctggttta cctgtcctac tttttcagca atattcatcc tcgaaagtaa
    23941 atcttctact ctttggcgga tggttaactt tgggttttga tacgggtaat cgtacacctt
    24001 aatatcctcc ttctgccaat tttgccttat tagcggctaa tacaaatggc aggaataaaa
    24061 atacatctag aatgataagt attactgtta agactgctgc tcgtatatcg cctcctgtag
    24121 caataaaaga ttgtataacc ggtggagtga tccaaggtgc agcagcaact gttttactga
    24181 tgattccaag acttgtggct gcgtaggcaa taaccaagtt aatagatggt ataatagcgc
    24241 atggaatcat atatattgga ttcatgacta ctggtaatcc aaacataatt ggttccgtaa
    24301 tattaaataa acaagtagtc agaccaaatt tagcgattga tcgctgttct tgacgtttag
    24361 aagcccacaa gatagcaata actaacggta aaatacaccc gccacctccg atcaatccaa
    24421 atgcattggt aaagggctcc gtaataatat taggaatttc ttttcctact gaaaaagcat
    24481 ccatgttttc ctgaatagac tgtaacaaag gtgcttgccg tataggagcc aaaatactcg
    24541 aaccatgcat accaaacacc cataagaaat cagagaaaaa ctgtaaaaat aagatgcctg
    24601 ggacggataa aacaaaagct ttcaatggtg tctgaatcgc taattcaatt aattgcggaa
    24661 cattcaaacc agtcgtatgg tttaaagtaa aaacgacaac tgaaaaaaat aaaatgacaa
    24721 ctatttctgg tactaaagaa ttaaatgacg cagctactgc cggtggaact ccttctggca
    24781 tcctgatttt aaaactcttc attttcgcta atttgataaa taattctgta ctgacaaggg
    24841 cagcaatcat cgccgttgct aagccagacg aagaagtcag actttgtaag aaaacccctg
    24901 tagcttctgc tgtatttccg tccatcaaag tgactgtact tgataaagac atcaaaataa
    24961 acattacggc tgtacttaga attcctgcat gaacaggact aaatgatgga tcatcaatca
    25021 cgcctgtttg gatatagtgg gttgccaaat tccagccgat attgtaacac acgataatag
    25081 ctaaaatact catcgtacca ttattcacga ttgaagcaag ttcagccaat ggacttagat
    25141 caagtattct ttggactgtt gcatttgaaa aaatcagagc attcagcaaa accatgacag
    25201 aagcagccat tactaacggc atagtaacga taaaactgtc tcgaatggcc agcaagtgtc
    25261 gctggctact catcttagat acgattggta gcattttttc agatagtctg tctacgactt
    25321 tcattatttc atctcctaga ttctaaaatt gaaccggttc atttttaata tagcactttt
    25381 tttaaagatt gcaacacatt tttttaaaac gctttctaaa ggtggtggat tttttatgac
    25441 ttttgaaaaa ttattctatt taatggtatt ttgtgaattt tttctcttcc atccgtttga
    25501 agttttactt tccaatggta taatttcttt atcctatcaa atgggggaag tattttgaag
    25561 aaattaatcg tactatttat tttgtctgcg ggacttttga cattgactgg ctgtgatttg
    25621 tttggcggct ctgaccaatc ctcggacgaa acaaccgatt cttctgtctt aaaagcggaa
    25681 aaagcagata tcaccaacag tgtatatacg ctaaaagtac gtaaagatat ggatttatcc
    25741 gcgggacagt tgacatttac agataaaaaa atggaatgga cacgcacgta tcagacacaa
    25801 ggagctgatt cttctgaatc atctgaagaa aaaacagtgc tgacagatat caagattact
    25861 accaaagatg acgaatatat tatttcagga aaagaaaata aaaaagaagc aacagtcaca
    25921 tttaccaaaa tcggtaatta ccgtttgaag gataaggatg gcaacatcta cagtctatag
    25981 tgccatctgt tggatcttct tttgtttctc cccttctttt ctgctatagt aaagaagcaa
    26041 gatgactgta aaggagacga aatggatgat cgatagtcct gattattgta catgtaataa
    26101 caacaaaaat atccatacag aatttaatga tcaaggaagt ttgagtatat gcgacaattg
    26161 ccacaaacta gtcaaagatt cttttaaatc gtttgaatct tatcctgtca aaaattatgt
    26221 aaaaagcaaa agaagataag caaaatcaag caaaactcac tatggctgat ttttatgaac
    26281 ttcaaactca gcttcttcct ttgatcaagc ttcaaaaact ggaatcagta aaacaaacag
    26341 tggatcattt cagttcacaa atcaaagagt aacgttttat ttatttcaca tctttgaata
    26401 taaaataacc aaacgataga aagacttatt
//
LOCUS       DAZKKW010000035        25824 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.035, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000035 DAZKKW010000000
VERSION     DAZKKW010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25824
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.035"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            141..2201
                     /locus_tag="ACG8PO_001384"
     CDS             141..2201
                     /locus_tag="ACG8PO_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HFQ0206721.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            2284..2853
                     /locus_tag="ACG8PO_001385"
     CDS             2284..2853
                     /locus_tag="ACG8PO_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0206722.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(3028..3834)
                     /locus_tag="ACG8PO_001386"
     CDS             complement(3028..3834)
                     /locus_tag="ACG8PO_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0206723.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            3972..5486
                     /locus_tag="ACG8PO_001387"
     CDS             3972..5486
                     /locus_tag="ACG8PO_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HFQ0206724.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            complement(5889..6872)
                     /locus_tag="ACG8PO_001388"
     CDS             complement(5889..6872)
                     /locus_tag="ACG8PO_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HFQ0206725.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(6877..7524)
                     /locus_tag="ACG8PO_001389"
     CDS             complement(6877..7524)
                     /locus_tag="ACG8PO_001389"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding oxidoreductase"
                     /protein_id="HFQ0206726.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            complement(7609..8241)
                     /locus_tag="ACG8PO_001390"
     CDS             complement(7609..8241)
                     /locus_tag="ACG8PO_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFQ0206727.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            complement(8257..8808)
                     /gene="coaC"
                     /locus_tag="ACG8PO_001391"
     CDS             complement(8257..8808)
                     /gene="coaC"
                     /locus_tag="ACG8PO_001391"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HFQ0206728.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            complement(8805..9542)
                     /locus_tag="ACG8PO_001392"
     CDS             complement(8805..9542)
                     /locus_tag="ACG8PO_001392"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HFQ0206729.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            complement(9539..10528)
                     /locus_tag="ACG8PO_001393"
     CDS             complement(9539..10528)
                     /locus_tag="ACG8PO_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFQ0206730.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            10786..11328
                     /locus_tag="ACG8PO_001394"
     CDS             10786..11328
                     /locus_tag="ACG8PO_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFQ0206731.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            11345..12430
                     /locus_tag="ACG8PO_001395"
     CDS             11345..12430
                     /locus_tag="ACG8PO_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0206732.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            12442..13248
                     /locus_tag="ACG8PO_001396"
     CDS             12442..13248
                     /locus_tag="ACG8PO_001396"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0206733.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            13249..14076
                     /locus_tag="ACG8PO_001397"
     CDS             13249..14076
                     /locus_tag="ACG8PO_001397"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0206734.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            14073..15146
                     /locus_tag="ACG8PO_001398"
     CDS             14073..15146
                     /locus_tag="ACG8PO_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0206735.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            15574..18270
                     /locus_tag="ACG8PO_001399"
     CDS             15574..18270
                     /locus_tag="ACG8PO_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFQ0206736.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            18335..18835
                     /locus_tag="ACG8PO_001400"
     CDS             18335..18835
                     /locus_tag="ACG8PO_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFQ0206737.1"
                     /translation="MIKPMFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLS
                     SIQLLTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITK
                     TFLEKEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVN
                     EEEDFF"
     gene            18847..19341
                     /locus_tag="ACG8PO_001401"
     CDS             18847..19341
                     /locus_tag="ACG8PO_001401"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0206738.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            19362..20171
                     /locus_tag="ACG8PO_001402"
     CDS             19362..20171
                     /locus_tag="ACG8PO_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFQ0206739.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            20168..20989
                     /locus_tag="ACG8PO_001403"
     CDS             20168..20989
                     /locus_tag="ACG8PO_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFQ0206740.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            21040..23139
                     /locus_tag="ACG8PO_001404"
     CDS             21040..23139
                     /locus_tag="ACG8PO_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HFQ0206741.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            23136..24254
                     /locus_tag="ACG8PO_001405"
     CDS             23136..24254
                     /locus_tag="ACG8PO_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 172 protein"
                     /protein_id="HFQ0206742.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            24293..25171
                     /locus_tag="ACG8PO_001406"
     CDS             24293..25171
                     /locus_tag="ACG8PO_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFQ0206743.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            25317..25514
                     /locus_tag="ACG8PO_001407"
     CDS             25317..25514
                     /locus_tag="ACG8PO_001407"
                     /inference="COORDINATES: protein motif:HMM:NF016526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HFQ0206744.1"
                     /translation="MLLRFLFSFCCALYTLRIFTGGVHLNSNILCYFMSVVIVIVAYQ
                     CMFIFRNVDFIILGLMFEFFW"
     gene            25498..25728
                     /locus_tag="ACG8PO_001408"
     CDS             25498..25728
                     /locus_tag="ACG8PO_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0206745.1"
                     /translation="MNFFGDRFFQPYKINRNSIIESCDYKKIARKKKKVSIHFKYFIP
                     ISIAKKVEILYLPLTLASLANVVSILIDNKLR"
BASE COUNT         8171 a         4482 c         5441 g         7730 t
ORIGIN      
        1 cgattttctt gaaacaataa aaaatcaaaa aaaatactct cctaatagtc catcttcttt
       61 tgattttatt tatataatga aatcatctta tgaaaattac tacttcatgt ttagacaaga
      121 gtagggagga ctaagaataa atgtgttcaa aaagacgttc aaaacaacta aataaacacg
      181 cttgtcgttt agataaggaa cggtcgtgtt ctgaaaaaca ctacgttaag tttatgatgc
      241 gcacgatgct gatcagttta tctgtaggtg tctatatgca tgtacccgtt caggcaacga
      301 cacaaaccat caaggtcgta cgtacctctg tttctgagac cactgattcc cctatcacta
      361 ttactcatac tttcactgat gcaaatggaa aacagctact tcctgcattg acttcctata
      421 ataaagtcag ttatcctcca acgataaacc actactggtt aattcctaac aaaacattat
      481 ggacatcgga aaccggaagc agtgtgccgc tttctatctt ttccgatatg actgatccta
      541 caaaaggtga tcagttaaaa cagatcactg atattttgaa tcatgctttg acagatataa
      601 aggacggtgg aagcgcacaa gtttcttatg tatatgaaaa agattattca cagtttgaaa
      661 gaaagaaaat cgatgttctg gcagggacaa aagtttcgat atctgatctg gtagaaaggc
      721 tgaagaacgc agatggaaca caaggagata tgtccaaagt aatttcaaac aatgggaata
      781 tcgtggatac gactaataca ggaacatcta ctatttcctt gtcctatttt gatccaattg
      841 ctttaaaaac aatgactgca tctgctgtga tccatgttcg tgctgatcaa acagctatca
      901 aaggaaaaga tcttcactta agaacaggag agagttacgc ccctaaagat cttgttgaat
      961 atgtaacaaa aagcgatgga acttcaggaa ataaagaaga aatcaaaatg aacgggacag
     1021 tagatactac aactccggga gaatatcccg ttgtattaac ttatatagac tcgaaaacat
     1081 caaatgtggc agaaacaaca gctgtagttt atgtagaagc atctgctgct tcatcgactg
     1141 tcatcactca ttttatagat agtcaaacaa ataaagaact tcaagcagaa acacatatga
     1201 gtagtgtaaa tcgtattcct gatttgcgcc tacctgctga attttctgat cgagtgccaa
     1261 atatagattt atcgacttat gaacaaaatg ggaaagtcac ttctatttct gattggatgc
     1321 aagcaaacgg attagacaga aaaagtcact ggtcggagat ccttgaagta cttcactcag
     1381 aactgatgaa taataggaag caatcaggag gggaaattac ctttacttat gtttatatac
     1441 cagacaatcg ttcattagaa ggtcatgatc ttattgttca tccaaacgaa aaaataaaca
     1501 aagaagagtt gatccaatca gcaacaaatt ccttaggtaa acccgtaccg ttaaaggatg
     1561 tatcggttac aatcaatgaa gtaccattgg aggatggtta tttgaccaaa caaacaggtg
     1621 attttacgat tatgtatagt tatcatgacc cgtattcact ccaagaactc catgctcagt
     1681 cactattaca tgttttacca ttaaacaaac agatagatca aaaagaacaa aaaagacaag
     1741 aagaaagctt gtcagaaaaa aaacaacaaa tggcccatgc agtttctcca acagggaaag
     1801 taaaacaaac aagaagccta gagttagcaa gaatatctag aaaaatctca gaatctaata
     1861 aaacgataag gcaagaagct catttggccc cacaagtttc tggattggaa aatgaaattt
     1921 tagagcctaa aataaaacaa caatcagaaa aagaatttgt tgctaaacag tcggaagatc
     1981 agaaggaatc agatgaccag aaggaaacga agaaaaaacg cttgtcaact attgattcca
     2041 ccgatgagca tctgcagccg caagtaccac ttcctagtcc gggaacaccc gcacctggtg
     2101 gaggtattcc ttcttctgct ttaggagatt tcatgcgagg tatggcagga acatgggtat
     2161 acgctaatcg aaatgaattt gacgcagatg actggttctg atttatatct ctatctgtta
     2221 tagtcaaata agtagctgag tcatatgtta tttggcataa tggagacgag gagggataga
     2281 aaaatgttag ataaaacgtt accttacgca gaaatctgga tgacaagacc acttcaacaa
     2341 aatgttccaa tcacgccttt agcggaaggt tttgaatttc gattttatca aaaagaagat
     2401 gaatctgctt gggcggagat tgagacttcc gttggggaat ttcaaacgac agatgaagca
     2461 cttgagtatt ttcaacaaaa ctttgctcct tatcctgaag aacttgaaag aaggatgttt
     2521 tttgtagaga caaaaaaagg agaaaaagta gcaacctgta ccgcttggcg aaaaaaatca
     2581 agagagcaag tgtatccggt ttttcattgg ttagcagtga aaccagatta ccaaggaaaa
     2641 gggctcgcaa aagcattgac tgccagagtc ttgcaagaat ttcctaccct ccatacggac
     2701 ggaccaatct atcttcacac acagacatgg agccatcagg cgattgctct ttataaaaaa
     2761 atgggtttta cttttattgc tgaaaatcta gacggcacac ctaatccaga ttatgaaaaa
     2821 gttatgcagg ttttaagtaa attagataaa taacgaaatg gtgaaataag tcctcagtat
     2881 tgggataaaa aacgaaatag tcattcctca tatcttactt ttttgagtta atactatcgg
     2941 ataactaatg gcatgccata cattcaaaaa agaaggggca gtgacaaaag tattgtcact
     3001 gccccttctc ttgtttttta gcatgtttta tcgtttgatt tttttaggaa taaaattctt
     3061 gatgaacgta aatttttggt aatcgtttcg actatagagg taaccaaata cactgaaaac
     3121 aactgctcca ataaaattaa caaataaatc cttcatagta tccatgattc ccacgtctaa
     3181 gtaaccattt tggatgattg ttttatctgt tttaccatct gcattttgac tttcaatgat
     3241 tgtccgagtg atatctggaa catgatagac agtgtttccg tctttactta actccacact
     3301 attgatttgc tggatgatcg tatctttttg catatcgaat tgcagtaatt tatctgctga
     3361 aaattccaca aactcccaca taacaccaac tgtcatcgaa aaacagaagg aaacaactgc
     3421 tagtaataaa ggagtcaagt tcatggtttt tgtattttta ttgagcaaat aaacaaggga
     3481 aaagccgaca cccccagcta aaaatccgct gaatgcatgt agaaaagtat cccacattga
     3541 aaaatagcca taaaaatcac ttaactctcc taaaactgtt gaactaaaaa taaaagataa
     3601 caaaatgatt tctagcatat ttggaatctg tatctttaat atcttttcca gaaaataagg
     3661 gatttcaaat aaaattaatg tcagtaataa aagcattgta tttccccagt gttggttcca
     3721 gacctgatcc actagcataa taacgacgat gatacgtaat aaaatataga ttccgtttgt
     3781 gattctacct gcagtttttg tactgttcgt aaaaaaggtt ttcaaatgat tcataaaata
     3841 actccttcat gtatatcctt aattatacta agaagcgtgt gtttaccaaa gctatatgac
     3901 ttaaagaaaa acagagttga tattgtgaaa aaaatcactt aatatagtat aaggagaaaa
     3961 gggggataca aatgaaaaaa ttagttgctg gtttattaac agccgttgca cttcttgcta
     4021 tgagcgcatg cggcgcagat cagaaaaata cagcgcagac aagttctagt acgaaggaat
     4081 ctgccgaagt atcatcaggc gcttctgaac aagtatatac agatccaaaa gaaatgaaag
     4141 aatcttatga tgtcgtgatt attggagctg gtggtgcagg gatggcagca gctatcgaag
     4201 caaaagatgc tgggttgaat ccagtaattt tggaaaagat gcctgttgcc ggaggaaaca
     4261 catctaaatc atcaagcggt atgaatgctt cagaaacaaa atttcaaaaa gaagaaggaa
     4321 tcaaagactc gaatgatgcc ttctatgaag aaacgctaaa aggtggtgga ggaacgaatg
     4381 ataaagaaat gcttcgatac tttgtcgatc actctgctga tgcggtcgat tggctagatc
     4441 aaaacggtat caaactggac aacttaacga tcactggagg gatgagtgaa aagaggacac
     4501 atcgtccgtc tgatggctcg gctattggtg gatacttagt ggaggggtta ttgaataacg
     4561 tccatgagcg ggaaatccct atttttgtca acgcagatgt gacagacatt aagaaaaatg
     4621 gggaaacggt caatgaagtg actgtacatg ttcaaggaga agaaccaaaa gaggtcaccg
     4681 gaaaagcaat cgtagttacc acaggcggtt ttggtgccag caaagaattt attgaaaaat
     4741 atcgtcccga tttagctgat tatattacga caaatcaaga aggatcaact ggagatggta
     4801 tcaccatgat tgaaaaatta ggtggacaaa ccgttgatat ggataaaata caaattcatc
     4861 caactgtgca gcaggatgag ggggtcttga ttggtgaggt tgttcgcgga gaaggagcaa
     4921 tcttggtcga tcaagagggg aaaagatttg tcaatgaaat ggatacaagg gataaagtat
     4981 ctgcagcaat tacagcattg cctgaaaaat cagcttattt gatttttgac caaggcgtgc
     5041 gtgatcgtgc cacagctatt gaattttatg accaaaaagg atatgtcaca actggaaaga
     5101 caatggaaga attagcagaa aaaatcgatt taccaaaaga aacactagca aaaacagttg
     5161 atggatggaa tacggatgtt gcggagaaaa aagatgctca attcaaccgt gccacagcca
     5221 tggaacatga cttatcgtct ccgaattatt atgcgatcaa aatcgcacca ggaatccatt
     5281 acacgatggg cggtgtgaaa atcaatacga acactgaagt attagataaa gaaaaccagc
     5341 caattagggg gttatatgct gctggtgaag tcacaggtgg cttacatgga aacaaccgga
     5401 tcggtggaaa ttcagttgcg gagatcatta tttttgggcg acaagcgggc atacaagcag
     5461 ctcgatttgc ttcggaaaat gaatgaaata gaacgtaaat aaaaagaggt tgtgaaatca
     5521 gcgttttgag ctaataccga gggtcagctg attgaacact gtttagtgaa gaggttgtga
     5581 aaaagctgcc ctgcatcaag taataagaac agccaagtaa aaacagcttc ttatgttttt
     5641 aacttggttg gacttattac cgcagatgca agcttttgaa caccgtttat tcggaaaaag
     5701 agcgtgggac aaaagtaaag aacacttttg tctcacgctc taaacacgta taaacggcgg
     5761 aagcagaaga aattccttcg gaaataagct gaaattcaca aaaatttgaa aagcaatttt
     5821 cgtgaatttc ttcttatttc ttggagttaa acgcttctgt cccagcctct tttttgatca
     5881 gcatgatttt attcagttat cttcaaaatg atttttccga tgtttttgtt tgcttccata
     5941 tattcatgcg cttgtttgac tgcgctgaat tggaatgtac ggtcaatgat tggccgaatc
     6001 ttgccatttt ctagaaaagg cccagctttt tgcataaaag tttgtgcgag ctctgcttta
     6061 tactgatcac ttctaggtgt caataatgta ccaagcagtt ggacacgttt cccaatcaat
     6121 acaccaaggt ctatctcagg cacgatggtc ccacctagca taccaatcaa gacccaacgt
     6181 ccatcaacgg ctatagaagc taaattcttt tcccagtaag aagcgccgat gaaatccaaa
     6241 ataacatcga ctccccgacc ttcagtgatt tttcgtacct cttcttgaaa gtcttgtttt
     6301 ttatagttga tcaattcatc tgcaccaagt tctttacaaa aggcgagttt ttcttctgat
     6361 cctgcagtaa cgatgatttt cgcgtgagaa agctgtctag ctaattggat cgctgctgtt
     6421 cccacaccac ttgctcctgc atggatcaag acagtttcgc cttcttgtaa ttttccaagc
     6481 cagtaaagtg tttgataagc agtcaaaaaa acttctgaga ttccagcagc agaaacatag
     6541 tctagcgtat cagggagaag gattgcacga tcggcaggca tcacagcata ttcggcataa
     6601 ccacctagat tgactaatcc gtatactttt gtcccaggag taaaagtgga gatatcactt
     6661 ttattctcaa ctacctctcc agctacttcg actcccaaaa tagggtaggg aggtttcaaa
     6721 gagactgatt ctcttttcat aatatctgtt cgattgacag ctgctgtatg gactttgatc
     6781 agtaattcgc cttgttttgg tttaggaaca ggcacttcaa tttgttctaa tacatctaca
     6841 gaaccaggat gcttgattga aatggcacgc atggatctat tcctcctttg cccattcttg
     6901 gaatgctgta ttcaactctt tgctaccttc agaaagtact agagtcgtac gagaaatatc
     6961 cgtttcttcc actagagcgg caagtacagc agcaactgta gagcgagtga tcgtttttga
     7021 tgccttctca ttaggcttag ttaattgaat ggaagtaact tcttcatcgt tagtcaatgt
     7081 gactggttga agaatgacaa aatctaagtt agttcgtttc agccattcat ctgcataatg
     7141 tttagtaata taataatctg tcatggactc aggccatttt gctggttcat ctgcataaac
     7201 agcactgacc atcagatagc gagagacatg cgcattttca gctgcgatca ctgttttgat
     7261 tgcgccatcc aagtctactt gcagaagatt ctttccttga gagccggcag caaaaatcaa
     7321 tacatcgatg cctttgaatg cttcggtcat tttttccacg gaccaagtta aatcaaaaga
     7381 gacatacgaa acattctctt tttttactaa tgtttgggaa gcaacatctc tcacccctgc
     7441 aaaaatttca tgggaacttg gagctaaatc ttttatcagg tgtcggccaa tttgtccgtt
     7501 tgcacctaca acaaaaattt tcatttggat catcctttcg acgtcaaatc tttccattca
     7561 atagtaacac caaaagagaa aaaaatcaga gatcggctac gatgactatc agttagtaag
     7621 agaagggtgc ttgaagtatc ttaacaagac attgcatact gctgcagtcg caagacctgc
     7681 gacgattcct tcaggtatac cacttgtaaa aataaccgct agaatagctg aataaacagc
     7741 atttaaattt gttccgatga ctttggcata atcttgctgg aataaaaagt agatcatatt
     7801 catcactaaa atcgtattgg tcaatgaacc ggaaagtcca gctaagaata aagaaacgga
     7861 atctgctttt tgtttcatta gtttgcgcag acctttataa acgaaataag gaaccacccc
     7921 aatcaagatg cgaggaataa acgcgatcag caaggctttc cagcttccat gttctgttcc
     7981 gatgactggg ataaacggag aaaagacaaa agacaaaggt gtttggataa ctgtgctacg
     8041 gatcatgctg atcagtccaa atgtaccgcc aagaaaagca cctagtcttg gtcctaaaac
     8101 gatcgaagca atgattacag gaatatgcat ggttgttgca ttgattggac cgatagggat
     8161 aaaacctaaa ggcgtaacag ctaataaaat caaaatagct aaaaacatcg cagttaatgt
     8221 aaagtttttt gtatttttca taagtactcc ttttttctaa gcatttcttt cattaagagt
     8281 cagcatgacc tgttcgatga tcgtctgaat atcagctaag gcaccttttc caaaatctcc
     8341 acaagctaaa agagattctc ttggttcaat ttcctgatag ccgacagatc tcaatgtttc
     8401 cagattacgc tgagtgatcg ggttttgata catattcgtg ttcatggcag gggcaatcaa
     8461 ttttggtgtt cccactggaa gcgccattgc gactgttgaa agaagatcgt ctgcaatgcc
     8521 gtttgctagc ttgccgataa tgtttgctgt agcaggcgcg atgagaaaca agtcagcttg
     8581 ttttgctagt tcgatatgat tgattacctg cggatttgat tcttgcatga cgtctgtatg
     8641 gactgcgcgc ttcgacagag attgcaaggt taatggcgtg ataaaatgtg tgctgctttt
     8701 agacataatc acatctacct gggcacctaa tttgacgaat tgacttgtaa gatcagcgct
     8761 tttgtaagca gaaatacttc cagtaacgcc tagtaagatc tttttcattt tgattcctcc
     8821 agtctaattt tctcagtaat caatgcagca atctcagatt tactttgagc ttcttccact
     8881 gttccatctt tagataagag atagccgtgg tgtgtctcgc catggatttc cgtcaaatca
     8941 tttgcgaaaa taaaatcagt tttgttattt atcagactat tttttgcgac tgccagaaga
     9001 gattcctttg gtacatctac taatagtttg aaaccaatga tttttgcttt tggctgccat
     9061 tcacgtagat aagaaatgat ttttggtgtt ttcttcaaaa cgagcacaag atgctcggtg
     9121 ttagaagaga tctttgaaac agtggtatct gtctgttcat tttccgcaaa ccaattgtcc
     9181 agttcttctt gcgttgaaga agtgggtagg ttttttgcca gctgttcttc tgaaaaacta
     9241 aaagcaggag taaaatcact gactgccata ctgtggataa tcgcatcgta gtgttctttt
     9301 ttcgttagtt cttctaattg taaaaataag tccagtgcag attcgatcgt ataaatcgtt
     9361 atattggagt gtcttttcgg tttcagtgct tgtttcgtag tcacgtaatc gattgtggta
     9421 tccggattgg cagctaaata atcagctaat gcttgcccta atcgtcctgt cgaatgattt
     9481 gtaatagcac gtacttgatc gattttttca gtcgttcctc cagctgtgat tagaatcttc
     9541 atttatggtc acctacattt actagtgttc ttccttggtg tgttccagca agtagtttag
     9601 atgctgtttc aggcaattga tccagagtaa tctcttggac gaccagttca tctgcaatct
     9661 gcaaatccgc catctgttgc cataaagaaa gacgttgatc tattggtaca ttgacagaat
     9721 caattccaat caactggatg tttcgcagaa taaaaggcaa gacagtcgcg tttagtttga
     9781 ttcccccagc attgccacat aaagctactg caccgttata agagatcaat ggaagaagac
     9841 tgcttagctg ctcgccacct actgtgtcga tgacatagtc gatttgctgt tttcctaaag
     9901 cttttgttgt ctctggcaga aattcatcag gtcgtacgac ttgcaaagca cctagacttt
     9961 ttagccatgt gacagactct tcttttctgg aaaaggcaat gattgactgg aatcctagtt
    10021 tatgtaacag ggctatcgct gtactgccta caccgccaga agcaccagta acgacaactt
    10081 ttttatcgga ggtcatacct tgacggatta aagcgttcac acacaacata gcagtaaacc
    10141 ctgctgtacc aaggatcatc gcttgacgca gcgtcatatt ttttggtaga ggaactaacc
    10201 agtctcctgg tactttttga tattggctat aaccgcctgg atgggtgacg ccaagcccaa
    10261 aaccagtagc aagtacaaga tcttcttttg aaaaacgtgg attgttactt tggacgacag
    10321 ttccggataa atcaatccct ggagtcatcg gatatgttcg tatcacctga ccagactcgc
    10381 tcattgccaa tgcatcttta taattgacat cagaataggc aatttttacc aagacttcat
    10441 tttcagccaa ctcagtaaat ggcagatcac tgattgctgt atggaaaaca ggttccttta
    10501 ttacctgaaa agatcgaaaa gcgttcattc gtgagacact ccttttaaaa ttttctttta
    10561 tcataccata aagcctactc ttttattctt gttcttccgt aaatttagac aaaaattagg
    10621 tgaaaaataa agaatcaaac aactagaatt tattccaaaa gataaagaaa tattcagcaa
    10681 aaaaaatttt acaaagggtt gcctttttca ataaatttcg tatactaata cgagtggtag
    10741 taaacaggaa gtttactatt agaaaacttc gattcggagg aaattatgga aattggtgag
    10801 aagttaagaa atttgcgcat ccaaaagaat ttgacacaag aagaactagg cgaacgcaca
    10861 gatttgtcaa aagggtatat ttcccagttg gaacgagatt taagttcacc atcaatggaa
    10921 acattctttt ctattcttga agtcttaggt gtgactccag aagaattttt tcatcaagaa
    10981 gcagcgaatc tgcaagtggt ttattcaaaa gaagatcata cggtttattt cgatgaagaa
    11041 aacgggtatg aattggaatg gctcgtggct gattcgaatg aaaaagaaat ggagcctgtc
    11101 ttgctgactt ttgaaggtgc aggagaatat aagacgtttg aaccttcatt atctgaaacg
    11161 tttatttttg tattagaagg ggaattagag ttaagcttag gagagacggt ctatgtggca
    11221 aaagcaggac agtcgatcta ttatcaagcg acaaaacaac atcaattaag gaatcatctc
    11281 aaaaaacgaa caaaagtatt gattgttgca acgcaatcat atttgtaata tagggggtaa
    11341 aaacgtgggg aaaccaatca tttcatttga tcaagtagtg aaacgctatg atgatgaaac
    11401 agtattaaaa aaagtaagtt ttgagatcga acaaggaaaa ttttatacac tgcttggtcc
    11461 ttccggctgt ggtaaaacaa cgatcctacg tattatagct ggattcaccg aagctacaga
    11521 aggggacatc tattttgaag ggaaacggtt gaatgatttg ccagctaata agcgacaagt
    11581 aaatacagtc ttccaagatt atgcactttt tcctcatatg aatgtttttg ataacgttgc
    11641 ttttggattg aagatcaaga aaatgtccaa atcagatatc gaaaagaagg taaaagatgc
    11701 tttgcgtatg gtccagcttc ctggatacga aaaacgggag attagtgaaa tgtctggtgg
    11761 tcaaagacag cgtgtagcga ttgcacgagc aattgtaaat gaaccgaaag tattgctatt
    11821 agatgaaccg ttatcggcgc ttgatttgaa actacgtaca gatatgcagt atgaactgcg
    11881 ggatctgcaa caacgattag gaatcacgtt tatttttgtt actcatgacc aagaagaagc
    11941 gttggcaatg agtgatgaga tttttgtcat gaataaaggg aaaatcgtcc aaagtggtac
    12001 acccgtagat atttatgatg agccaatcaa tcatttcgtt gctgattttg ttggggagag
    12061 caatatcgtg gacggtgtaa tgatcgaaga taatctagtt gagtttgttg gtaagcaatt
    12121 tgaatgtgtc gatggcggga tgcgtctaaa tgaaccagtt gaagttgttt tacgcccaga
    12181 ggatttgacg atcacgacac cagataaagg aaaactagtc gtaacggtcg atactcagct
    12241 gtttcgtggt gtccattatg aaatcatctg ttatgatgaa cagcaaaatg aatggatggt
    12301 ccattcaacg aaaaaagcta aagaaggaag caaagtagga ttggcctttg agccagaaga
    12361 tatccatgtc atgcgtttca acgagtctga agaagaattc gatgcacgtc ttgacagcta
    12421 cgaagaatag gggggacaag aatgaaaaca atgcgcagga tctattccgt cccttatatt
    12481 ttgtggctcg gtttattcgt gatcgcgcct gtattaatga tcatttatca atcttttttt
    12541 gatatgaatg ggcaattcac gctgaataat tatctatctt attttacgtc ggggacatcg
    12601 ctttctatga cgatcaattc tgtatggtat gcatttttga tcacgttttt cacgctgatc
    12661 atcagttacc cgactgctta ttttttaaca aaactgaagc acaagcaatt atggctgatg
    12721 ctgattattt tgcctacatg ggtgaatctg ctgcttaaag cttacgcatt tattgggatt
    12781 ttcagtgtgc acggcagcgt gaatcatttc tttgatttta tcgggatcgg gccgaaacag
    12841 atattattca cagacttcag ttttctgttc gtggcaacgt atatcgagat tccatttatg
    12901 attatgccaa ttttcaatgc attggaagag atgaatcctt ctttgatcag tgctagtaga
    12961 gatttaggcg cgaatagctt tgagaccttc cgacgagtga tttttccgtt atcattaaat
    13021 ggtgtgaaaa gtggtgtcca agcagtattt attccttctc tttctttatt catgctgact
    13081 cgtctgattg gcggaaatcg ggtgatcact ttaggaacgg ctatcgaaca gcatttcttg
    13141 gttacacaaa actgggggat gggttctaca atcggtgtga ttttaatcat tgctatgttc
    13201 atcgtgatgc tcctgacagg cgagaagaaa aaaggaggtc gctcataaat gaaaaaattt
    13261 aaatggtcgt atctttattt agggatcgtc tttttattct tatatgcacc gatcttctat
    13321 ctgatttttt attccttcaa tgaaggagga acgatgaaca gctttacagg atttacttgg
    13381 gaaaattatg aagctgtttt tgaagataca cgtttgatca ttatcgtatt gaatacgttc
    13441 atgctggctt tcttatctgc attgatcgcc acgatcatcg ggacatttgg ggctatggga
    13501 atctattaca cgaaaaaacg acgtgcacgg acagcgttgt tgagcatgaa taacatctta
    13561 ttagtctctc ctgatgttat tatcggtgct agtttcttga ttttctttac actactagga
    13621 ttcggattag gttttcagag tgtattgctt tcccatattg ctttcagcat tccaatcgtc
    13681 gtcttgatgg tcttgccaaa attgcaggaa atgaacgatt caatggtcga tgcggctaga
    13741 gaccttggtg ctaataattt ccaagtagtc aaaaatatca ttttgcctta tctttctcca
    13801 ggaatcatag ctggatattt tatggcattt acctattctc ttgatgattt tgcggttaca
    13861 tttttcgtca caggaaatgg ctttacgacg ctctcagtag agatctattc tcgtgcaaga
    13921 caaggaatca gtttagaaat caatgcgcta agtgcgttag ttttcttgtt ctccatgata
    13981 ttggtcattg gatactactt tattagtaaa gaaaaccgtc caaaacggct gaagaaaaac
    14041 agaagagtac gcgagggggt ggcacggctg cgatgaaaaa attacaatct ctgtttttag
    14101 gtattctcat cattattgcg gtgctgctgc ttggtgttcg tcaacttgaa aaaacaagtg
    14161 gcatgagtgg agcaaaggta cttaccatct ataactgggg agattatatc gatcctagtt
    14221 tgatcacgaa atttgaaaaa gaatatggat acaaagtgaa ttatgaaaca tttgattcta
    14281 atgaagcgat gttcacaaaa atccagcaag gtgggacaaa ctacgacatc gctataccaa
    14341 gtgaatatat gatccaaaaa atgatcaaag aaaaactagt acttccatta gatcattcga
    14401 agatcaaagg attaaataat attgatccac gatttttaga cttggatttt gatccagata
    14461 atcagtattc gattccatat ttttggggaa ctttagggat cgtatataac gataaattct
    14521 ttgatgcaaa tcaaattaag cattgggatg atttgtggaa gccggaatta aaagacagtt
    14581 tgatgctgat tgacggtgca cgagaagtta tggggctttc tttgaatagt ttaggctatt
    14641 ctttgaatag taagaatatg gcacagctga cagaagcagc aaataaattg aatcatttaa
    14701 caccaaatgt caaagcaatc gtggcagatg agatcaaaat gtatatgatc aatgaagaag
    14761 cctccgtggc agtgacattt tctggcgaag ctgccgatat gatgtgggaa aatgaacatt
    14821 tacattatgt gatcccacca gaaggatcca atctttggtt tgataatatc gttatgccaa
    14881 aaaccgtgaa aaataaagaa ggtgcctatg atttcatcaa ctttatgcta gaaccggaaa
    14941 atgcggcaca aaatgcagag tatattggtt attctacacc aaatgaagaa gcaaaagcgt
    15001 tattgccaaa agacatttcg tcggacgaac aattctatcc atctgatgac acgatctccc
    15061 atcttgaagt ctacaaagat ctaggatcga aatacctagg catctataat gacctattct
    15121 tagaattcaa gatgtatagg agataatggc aaaactcagt aagaatgggg acttatcaac
    15181 gttctaaaga gctaaaaatc gtcctttttc tatctgagtt ttccaatctt ctaccaaaag
    15241 cggatgaaac gccgcttttt ttgtgttatt ttattttttc taccacgttt ttctaccaga
    15301 gaaaagtgga caaaaccatt gtaaaatagg gcccgagacg ggctagtaaa tctaagattg
    15361 aaaaagttac agaaggataa agtaattgga aactatgcaa atatacttaa cccttctgta
    15421 atctattaat aaaactaata tatgaatcca gttcaatatt tgtagctatg aaaaaacata
    15481 aaaatcatat tagataaaaa ctaaattggt agataaatta ttttgtcaga tatctgaggc
    15541 aaatattcga ctatttgtgt ttcaaagtaa aaattggaat acaaatatag aatagacata
    15601 ttgcagccga tagtttatta tgcttttaga cacattaaac tggaggaaaa aatgaaaaag
    15661 agcgctaaag atcgagtttt tgatgagctg atacgactaa cagggaatat gtcttctgtc
    15721 actgccgaga aaatttcgaa gaatctaggt atctctcgtc agaatgccag ccattatttg
    15781 acgagattag tggaagataa aaaggtcgag aaattaccgg gaaagcctgt tttgtggaag
    15841 cctttagatg aatatgcggt tgtagataat acggagcaaa taaacgaagc ctttcattct
    15901 gtagttggtc atgacggaag tctgagagag gtcattcaaa aatgtatagc cgctgtcaaa
    15961 tatccgccta acggactcag tgtattaata aatggagcca cgggtgttgg aaaaagtttt
    16021 ttagccacaa aaatttttga atacgctatc catgagcaaa ttattgaaaa agatgcacca
    16081 tttgctatct taaactgtgc cgattatgcg gacaatcccg aactgctttc ggcaacactg
    16141 tttggataca agaagggggc ctttacgggg gctgaaaagg ataccgatgg tttattggca
    16201 acggccaaca acggctatct ttttctcgat gaggtccatc gcctatcgaa agaaaatcaa
    16261 gaaaagctct ttctatttat tgatactgga aactatcgtc cagtcggtga aaatgtaaat
    16321 tggcattctg ctaaggttcg ttttattttc gccacgacgg aaaaaggtga aaattattta
    16381 ttagacacct ttgatcggcg tattcaaatt agtgttatgt tgccaacttt tgaagatcgc
    16441 cccattcgag aacgattaga gctgattcaa ttattttttc aaaatgaagc ggatgtttta
    16501 caaaaagata ttattgtttc aagagaagcc ttgtctttaa tgttatctca tccctttagt
    16561 gggaatattg gtaagctgaa gaatatcatt aaaatcagtt gtgcggatgt gtatagtcga
    16621 acacaagaag aaccattaac gattgggaaa aatgagatct tgattcagtt aaatatgctg
    16681 gaatccgaag tagaaagctt gccgattgct agtttattga taaagaaaaa tcaaaagata
    16741 aaaacggatc gaatcaaacc gtttgattat gattcgattt tggaaaaaat tactcagctg
    16801 ataaatgaag gtaatttcaa tgaaaatttt tcttcgataa agaatgaagt acagcatttg
    16861 gtgcatcaca ttgataagga ttctttttta tcaatgagta gaaaattgat gattcagatg
    16921 tttagaaagg tctgggtatc cgttttagga aaaacgtacg gattgacgac gagtggacct
    16981 gttgcagaaa ttattagcag atcctatgtc atttccccaa gctataagac agacttaacc
    17041 gggataaatc gtacattagc tgcaaatcta cctcggacga cttacttatg taatcagttc
    17101 ttggagcatt taccaccgct ctcagaaaag gacgagaact ttctaagact gcttattgtc
    17161 gtgactttat ctgattatgt agatgaatcc atcgagctga agggcctgtt gcttgctcac
    17221 ggagatagta cggctagcag cattcaagca gtagtgaatc aattatgcgg caattatgtt
    17281 tttgaagcgt tggatatgcc agtagagagt aatgtgtcag agattgtcga atcgacaagg
    17341 aatttcgtga aacggcaagt gaatacagat agtctagtgc tgatcgtaga tatgggctct
    17401 ttaaatcaaa tttacacaca gatcaagaac gatttaaaag gggagttact tcttctgaat
    17461 aatttgacga cttccattgc acttgatatt ggcttgaaga tgacgatgaa tgcgccattt
    17521 aaacagattg cggaggatgc agcagacaag tattcaatca atgttcaata ctttgaaggc
    17581 ttttctcaaa gtagcaacat tatcatttcc tgtatgtcgg gtttggggat ttcggacaaa
    17641 ttgaaagatg tttttcgtca tcatttatcg gatgaatcca tcgaagtttt cacgaaggac
    17701 tacagagaac tacgtgaatt aatcgaccaa aatgatgaaa cttattttga caagacaaaa
    17761 ttggtcatca cgacttcgga tcttccacgt tccttttcaa ttcctaatat taatgtttat
    17821 gacatcttag acggtgatgg agcagccaat ttggagcata ttttgagtcc tgaaatgaag
    17881 ccggcagaat ttaaccgctt gattcaggag ctagtaaaat tcttttcgtt ggaaggaatt
    17941 gcggaccgtt tgaactttct gaacccaacg atcgtgatga gtgaagtgga aacagtcatt
    18001 tctaaatatg aaaattacta tcatttcact ttaaatggcc gtgtaaaact aaatctctat
    18061 atgcatacgg cattgatgat ggaacgttta ttcttggctc ggacagaaaa atctgaagaa
    18121 ccgaattatg atttgaaacc agatgaagat gaattttatt atgtttctag aagtattttt
    18181 caatcaatgg agatgaaata taattttaaa gtcaatgctt atgagctttc gctgctctat
    18241 gaattattag gaccgtatat tcaaaaataa agtgtctaaa aaagtgtcta atctgttttt
    18301 tttaggttag acactttttt taatgtgaat taacgtgatt aaaccaatgt ttttaaaagt
    18361 tggcacagga attgcttata taaaagtgaa cagaaagaaa ggaattaacc atatgaagaa
    18421 aattctaata gcgactcacg gtcacttagc ttcaggcttt cttagcagta ttcaattact
    18481 gacggggaag gttcaagaga ttacggtgat taatgcttat gtggatgagt gtgattttga
    18541 acaggagttg aatgtatttg taaaccaggt gaacgagcag gatcagattt ttgtttttac
    18601 cgatttattt ggcggaagtg tcaatcagaa gattaccaaa acctttttgg agaaagagat
    18661 tgctgtaact attttgtctg gctttaatct gccaattctg ctcgaaatag tattggcaac
    18721 caaggaatta acaaaggagg atattcagga actggtaaat aaatgccaac aggaattaaa
    18781 gatgaccatt cttgatgagg tacatgtagt aaatgaagag gaagattttt tttaggagga
    18841 agaaaaatga ttaaagcagt gcgaattgat gaacgattaa ttcatggaca agtagcgatg
    18901 acttggacaa agagtctaaa tttaaccggg ttagtagtag caagcgatga ggcggctacc
    18961 aacgatattc aaaagatgac attgaagatg gctgcaccat cgaatgtcaa agtcattatt
    19021 aaaacagttg atgacgcgat tcatttgtta aaagatccgc gggcgaagga catgcgcttg
    19081 atggttttag tccctaatgt caaagatgcg gtaaaagttg cgaaagcttt cccaacggaa
    19141 atcgaattaa tgaacgtggg gaatgctggg aaaatgacac cgccatcgga agataagaaa
    19201 accttatcaa aggaagtgat gttaacgacg tcagaaattc aagacttaaa agaattagtt
    19261 gaactttatc cagataccgt ttttcagcct acaccggcga tggaaaaacg ttcagctatc
    19321 tctattttga gtgattttta gaaaaaggaa ggagaaaaat tatgtatgat gcatttgtag
    19381 tcgctttggc cgtttttatt ggagttgctg gacatgaaat gttcgggatg tccatgctga
    19441 gtcgtccgat tgttgttgct ccgttagcag gagcgttgat gggggatttg cagacggggt
    19501 tgattgtcgg ggcgtcacta gaagccatct ttatgggtgt cgtcaatatc ggaatctcta
    19561 gtacggcgga gccttctttg gctgctgggc tggctacagc gttcgcaatc aaaatgggag
    19621 gaaacgtgga tgcgattatt cccattgtct ttccactagc agtattagga ttacagttaa
    19681 tgaatatgat tttttctttt gtatgtggac cgatggctgc taaatttttc aaatttgcaa
    19741 aatctggtga tcaaaaagga atcatcaatc tgcacttctt tatgtggttt gtccactatg
    19801 cgctttatgc tcttattcca ttctttgccg ttttatttgg agcagatgtt gtcaaaagcg
    19861 tcttggataa tattccagag gtcatcatga acggattaac agtcgctgga aatctgctgc
    19921 cggcagttgg gatggcgatg ctgttacgga tgctttggag taaaaatatt gctgtgtggt
    19981 ttttctttgg ggccgttgtt atggcgtatc tcgagttacc attgattgca attgccgcag
    20041 ttggaacgat tattgcaatt gttatagccc aaagagatct acaactaaaa aatatatctg
    20101 ttcatgcagt tcaaagcgat gctgctgtaa atgcaacgct tgaccaagaa gaggaggatt
    20161 tctttcaatg aaattagctg atggaatttc taaagatgag aaaaaattgg ttcggaagat
    20221 gttttggcgc tcgatgacta tgtatgcttc ggtgaatccg gtgacgatgg gcggcggtgg
    20281 tttcgcctat tctatgatgc ccttcattaa tcatttctac aaaagtgaag aagatcgagc
    20341 tgcggcgctg gaacgtcata cacagtattt ttcgacgacc attccttgcg cgtcctttgt
    20401 catggggatt gcggcatcga tggagaagga aaattctgaa aaagctggtt ttgacgtgga
    20461 aagtatcaac gcgatcaaaa cgagtttgat gggaccattg gctggaattg gcgattcatt
    20521 gttctgggga gtttggcgtg tcgttaccgc gggactagcg atcaacttag ccaatgccgg
    20581 aaatattttg gcaccaatta tctttttagt tatgttcaat atacctaatt acttgatgcg
    20641 atactatggc ggcgttttag gctattcatt aggctctaaa tatattgaaa agctgtatgc
    20701 gaacgggtta attgaaattc tgacaaaagc tgctagtatc ttgggtctga tcatggtagg
    20761 tgcaatgacg atcagtatgg tcaaattcaa taccgttttg agcttttcaa tgaagggcga
    20821 agaactaatg aatgtccaag aaatcctcga tagtattttt aaaggaattg taccgttatt
    20881 ggtaacattg agctgtttca aattactaga taaaaaagtc ggcgtcatca aaattatttt
    20941 tggaatggtc gtcttgggaa ttttattgtc attacttgga atcgcatagt aggaagctgc
    21001 agctactagg ctgcagtttt tttagcaagg agggaaaaga tgactattaa tgaagtaatt
    21061 tattcaccca aacacccatt ttttaatgct gagttaaacg acggaaatgt gactacttca
    21121 aacgaataca gctactatcg gaatattcct gatgtaaagg gattgacctt cgatgtaagt
    21181 aatcaaaaga cattagcaaa ccttgatgtt tgcgggacga ttcaaacgtt gaccttttat
    21241 cgagaaaatt tactaactga ggaaaaacca ggtgtttggg tcaataaaca gctctctcaa
    21301 accaattcgt tatccttaaa ggttgaagta aacgggattg tctaccattt gtcagaatcg
    21361 gatcatcgta ttgaagtcga tgtgattcaa gactgtttgc ctagatatat tcatcattat
    21421 tcgacgttta aggtcattgt agtatccttt gctcccatac tggctaataa gcgtttaagt
    21481 atgcttgttc aacaggttta cattgtgaat gaaacacacg aaagccttca agtttcagtg
    21541 aaagatgtgc cttcgtatca agagaagtat tcggatcagc aaaatgtgtt gatttctcaa
    21601 aaaggaaata aggacacgat cgcacaacag gaagtggcaa gcttcgccat tgcttttgtg
    21661 gacccaaatg cttttgaaga agtaatgact tttcaggaat ctgatatcga aaaatggctg
    21721 aaggaaacat tgaactattt tgctcagcta tttgggcagc tgtcaatgcc aaacgataga
    21781 gttgtccatc tctatcgtcg tgcactttat caaagctttt ctagtttcgg tatgaatcga
    21841 cagggacagg tcgtcggcag caattggggc agctatcctg ctactaatcg aatttggaat
    21901 aaggatatgt attacgcatc attgccattc accatgtttg aaccggagtt atgtcaaaaa
    21961 acggttctat ggtttgatca atatggcgtt aagtttcctg gttcaaaatt tacaggaggt
    22021 atcaatcatt ctctgagtaa ttcgttatcc agtgcgttac tggctagtct ttattttgaa
    22081 catactgggg atacaaaatt ctttgagcgg tgtccgcagg ttctactgaa tgcttcaaga
    22141 attattgagg acatattagc tcaacgtcgg tcaggggaac caatgctttt tccatctgtt
    22201 tggttatcag acgcctttgc acttggaaag tatcataccg gcagcaatct ttgtctttgg
    22261 aaggcctgta ttggacttgc agaaattttt gaagccctag aacaactgag tcttagcaag
    22321 cgatacaaaa acattgcctg taaactaaag gaatcaattt gtaaacaaat gactatcgat
    22381 ggtccttttg gtgagcaatt tttggaggga atcggtgatg aggagaaaac aacctattca
    22441 gtgaagcact atcaaaaacc gattcttgag caagggttaa tctttttaag tgatgtgatt
    22501 gtggatggaa aaattaattt attgatgcat gatggagaag aatcagacac tacgttgatg
    22561 cctttttatc aatttttaga taataaagat catttatatc agaacaccat ggccttttct
    22621 gctagttctt tcaatccaac ctatagcgag gaaatcaaag gcatcacttg gggattagaa
    22681 tccggcgcca cttttcctgg ctttatcact gttttaatga gcgagctgca caaccctcaa
    22741 gctttcgtaa cccgattaga agaactaatt tgtttggcag atttagacgg ttcgtggtgg
    22801 tggtggccgt atcgcttaga ggctcaacaa ggagaagtcg tgcgagactt tggctgcggc
    22861 aaatgcggct gggcatcggg tctttttgtc agcctaatga tcagtcaata tttcggattg
    22921 aaattcaaaa aaggagtatt gcaaattgat cctgttgata atttaacgtt ttcttggaag
    22981 aatttgaagt tcggtcaagc cttcatttcg attgaatgta cgcaatccga gttgagtatt
    23041 tcaaatttgt cagaaaagcc attgaaaata agtgatacga aaaaaattct tcacgtagaa
    23101 aagggaaaaa cgattcacat tcaaaggaga aaaagatgag tatactaaaa aatattaccc
    23161 aatttaaaaa aatccagagt cgagcaatca ctcccgaaaa tccaacaggc gagaaaggta
    23221 aagctgcgat gttgccaagc gagctaggac caagccgcaa aggtcgtggc gcaattccgc
    23281 tgacttctgg tgaagaaact gtgattgcgg atattactgg aacgggaata attcgtcata
    23341 tgtggatgac gattcgtgat cgaacagaat ttggcagttt tgttttacgt gatttaatct
    23401 taagaattta ttgggatggg tcggatagcc ctgctgttga aagtccttta ggggatttct
    23461 tttgtaatgg gtttggagag cgttgcgagg tgaactcgtt gccgatagtg gttaatccta
    23521 cagggggctt taattcctat ttcgaaatgc ctttccaaac atccgctaaa attacgatcg
    23581 agaatttaca tccgaaggat atgtcctcat ttttttatac gataaattac gctttggtgg
    23641 acgaattgga agaaaatagc ctatattttc acgcaaattg gcatcgggaa cgcattacca
    23701 aattacagga ggattataag ttaattgatc gattaaaggg tcaaggctat tacgtgggta
    23761 cttaccttgc gcttacctgc ctagaacgtt attggtgggg cgaaggcgag tttaaattct
    23821 acattgatgg ggatagtgag tatccgaccg taacctcaac aggttcagaa gattattttg
    23881 gcggggcgtg ggcctttcat gagtacgatg agttcggacg tccacatgcg aaaaactatt
    23941 caacagcctt tttagggtat ccttaccaat caaatagaga tgccacaaga gatcgttttg
    24001 aaacgggaaa attgaatgcc gttcatgcct ttggtgacga cgcaatgcca cgacatggtc
    24061 tttatcgttg gcatcttcaa gaccccatct gttttaccga agatttatcg gtcactttac
    24121 aacaaatcgg caacgatgat gttcgcttat ttgagcggca agacgatgtt gctagtgtcg
    24181 cttactggta ttcaacagaa gcttcaggaa atactaaacg tcatttcact gatcgagaac
    24241 gcttgccgcg ttaatgaagt gtttttcaca tattcgaagg gaggaaccta tgatgatata
    24301 tggggcaatt gaagctggag ggactaaatt tgtctgtgca gtcggtactg aggaattaac
    24361 gattttggaa agagtcagtt ttcccacaac aactccagtt gaaacaatga agcaggtcat
    24421 tgattttttt aaacctttta aggaacaatt ggcaggcatc ggggtcggct gctttggtcc
    24481 aattgatatt caatgcaaat caacgagttt cggtcatatc acatcaacac caaaacttgc
    24541 ttggcaaaat ttcaattttg taggaacgct aaagcaatat ttcaatattc cgattgaatg
    24601 gacaacagat gtcaatgcgg cttgctatgg tgaatatgtt gctggttctg gcaaagggtt
    24661 agcaggtgtt gcatacttca ctgtgggaac aggtatcgga ggtggtgcct tgttcaatgg
    24721 gactttcgtg gaagggttca gtcatccgga aatggggcat atgttgatca agcgacatcc
    24781 aaaggacaat ttttcaggaa actgtccctt ccaccaagat tgtttagagg ggatggcagc
    24841 aggaccagcg attgaaaaac ggttgggtgt aaaaggccag aatttattgg ctgacgattc
    24901 gttttggcag attgaagcct tttaccttgc ccaatgtgca tacaatacaa cactgatgtt
    24961 ttcgccagat agaattattt ttggcggtgg tgtaatgaaa caagaacaca tgaagaaaaa
    25021 agttcaggat aaatttgttg agcttataaa tggatatgtt gaaataccac caattgatag
    25081 ctacattatt acgcccgaat taggtgataa tgccggaata atcggcggat tagcattggc
    25141 cagaaaagcg gtaagaaata agcaaccata attaatgaat gtttcattga tactcatgtc
    25201 aaaagtattt aaccgagtaa tcaaatgatc aaatgaagtt ggataaagcg aaaatgaatt
    25261 caaataaaaa ggctctaagt caaatcgtgt tgttttcatt aaatcactga aaggaaatgc
    25321 ttctgcgttt ccttttttct ttttgttgtg cgctgtatac cttgaggata ttcactggtg
    25381 gcgtacactt aaatagtaat atattgtgtt actttatgtc tgtggtaata gttatagttg
    25441 cttaccaatg tatgttcatt tttcgaaatg ttgacttcat aatccttggc ttaatgtttg
    25501 aatttttttg gtgatagatt ttttcagccg tataaaataa atcgtaattc tattatagag
    25561 agttgtgatt ataaaaagat agcaagaaaa aaaaagaaag tttctataca ttttaaatat
    25621 tttattccta tttctattgc taaaaaagta gaaattctat atttaccttt aactctcgct
    25681 tctcttgcaa atgtagttag cattttaatc gataataaac tcaggtagtt atattacctt
    25741 gaaatgattg ataattttgt cacttctatt ttttttgcaa taaatttttt attttagtga
    25801 aaaataagaa actatttctt tgaa
//
LOCUS       DAZKKW010000036        24705 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.036, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000036 DAZKKW010000000
VERSION     DAZKKW010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24705
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.036"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            8..81
                     /locus_tag="ACG8PO_001409"
     tRNA            8..81
                     /locus_tag="ACG8PO_001409"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:42..44,aa:Pro,seq:tgg)
     gene            101..173
                     /locus_tag="ACG8PO_001410"
     tRNA            101..173
                     /locus_tag="ACG8PO_001410"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:134..136,aa:Ala,seq:tgc)
     gene            201..274
                     /locus_tag="ACG8PO_001411"
     tRNA            201..274
                     /locus_tag="ACG8PO_001411"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:235..237,aa:Met,seq:cat)
     gene            288..361
                     /locus_tag="ACG8PO_001412"
     tRNA            288..361
                     /locus_tag="ACG8PO_001412"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:322..324,aa:Ile,seq:cat)
     gene            385..474
                     /locus_tag="ACG8PO_001413"
     tRNA            385..474
                     /locus_tag="ACG8PO_001413"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:421..423,aa:Ser,seq:tga)
     gene            487..560
                     /locus_tag="ACG8PO_001414"
     tRNA            487..560
                     /locus_tag="ACG8PO_001414"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:521..523,aa:Met,seq:cat)
     gene            579..654
                     /locus_tag="ACG8PO_001415"
     tRNA            579..654
                     /locus_tag="ACG8PO_001415"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:612..614,aa:Asp,seq:gtc)
     gene            656..726
                     /locus_tag="ACG8PO_001416"
     tRNA            656..726
                     /locus_tag="ACG8PO_001416"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:688..690,aa:Gly,seq:tcc)
     gene            740..813
                     /locus_tag="ACG8PO_001417"
     tRNA            740..813
                     /locus_tag="ACG8PO_001417"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:774..776,aa:Ile,seq:gat)
     gene            838..925
                     /locus_tag="ACG8PO_001418"
     tRNA            838..925
                     /locus_tag="ACG8PO_001418"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:872..874,aa:Ser,seq:gct)
     gene            947..1018
                     /locus_tag="ACG8PO_001419"
     tRNA            947..1018
                     /locus_tag="ACG8PO_001419"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:980..982,aa:Glu,seq:ttc)
     gene            1041..1130
                     /locus_tag="ACG8PO_001420"
     tRNA            1041..1130
                     /locus_tag="ACG8PO_001420"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1077..1079,aa:Ser,seq:tga)
     gene            1143..1216
                     /locus_tag="ACG8PO_001421"
     tRNA            1143..1216
                     /locus_tag="ACG8PO_001421"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1177..1179,aa:Met,seq:cat)
     gene            1235..1307
                     /locus_tag="ACG8PO_001422"
     tRNA            1235..1307
                     /locus_tag="ACG8PO_001422"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1268..1270,aa:Asp,seq:gtc)
     gene            1311..1383
                     /locus_tag="ACG8PO_001423"
     tRNA            1311..1383
                     /locus_tag="ACG8PO_001423"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1344..1346,aa:Phe,seq:gaa)
     gene            1388..1458
                     /locus_tag="ACG8PO_001424"
     tRNA            1388..1458
                     /locus_tag="ACG8PO_001424"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1420..1422,aa:Gly,seq:tcc)
     gene            1471..1544
                     /locus_tag="ACG8PO_001425"
     tRNA            1471..1544
                     /locus_tag="ACG8PO_001425"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1505..1507,aa:Ile,seq:gat)
     gene            1556..1627
                     /locus_tag="ACG8PO_001426"
     tRNA            1556..1627
                     /locus_tag="ACG8PO_001426"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1589..1591,aa:Glu,seq:ttc)
     regulatory      1816..1895
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01767"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="SMK box riboswitch (SAM-III) riboswitch; SAM
                     riboswitches modulate the expression of downstream genes
                     in response to levels of S-adenosylmethionine; SMK box
                     riboswitch shares no known similarity to SAM-I and SAM-II;
                     Derived by automated computational analysis using gene
                     prediction method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01767"
     gene            1893..3086
                     /gene="metK"
                     /locus_tag="ACG8PO_001427"
     CDS             1893..3086
                     /gene="metK"
                     /locus_tag="ACG8PO_001427"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFQ0206746.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            3216..4688
                     /locus_tag="ACG8PO_001428"
     CDS             3216..4688
                     /locus_tag="ACG8PO_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HFQ0206747.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            4771..5559
                     /locus_tag="ACG8PO_001429"
     CDS             4771..5559
                     /locus_tag="ACG8PO_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFQ0206748.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(5626..6294)
                     /locus_tag="ACG8PO_001430"
     CDS             complement(5626..6294)
                     /locus_tag="ACG8PO_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HFQ0206749.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQIMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            6409..8121
                     /locus_tag="ACG8PO_001431"
     CDS             6409..8121
                     /locus_tag="ACG8PO_001431"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0206750.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            8124..9893
                     /locus_tag="ACG8PO_001432"
     CDS             8124..9893
                     /locus_tag="ACG8PO_001432"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010762530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0206751.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENCKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFE"
     gene            complement(10098..10664)
                     /locus_tag="ACG8PO_001433"
     CDS             complement(10098..10664)
                     /locus_tag="ACG8PO_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFQ0206752.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(10664..11620)
                     /locus_tag="ACG8PO_001434"
     CDS             complement(10664..11620)
                     /locus_tag="ACG8PO_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HFQ0206753.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(11620..12276)
                     /locus_tag="ACG8PO_001435"
     CDS             complement(11620..12276)
                     /locus_tag="ACG8PO_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HFQ0206754.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            12646..14664
                     /locus_tag="ACG8PO_001436"
     CDS             12646..14664
                     /locus_tag="ACG8PO_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFQ0206755.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     misc_binding    14734..14940
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            15091..17505
                     /gene="leuS"
                     /locus_tag="ACG8PO_001437"
     CDS             15091..17505
                     /gene="leuS"
                     /locus_tag="ACG8PO_001437"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFQ0206756.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            17853..19406
                     /locus_tag="ACG8PO_001438"
     CDS             17853..19406
                     /locus_tag="ACG8PO_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40/YVTN/BNR-like repeat-containing protein"
                     /protein_id="HFQ0206757.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(19787..21571)
                     /locus_tag="ACG8PO_001439"
     CDS             complement(19787..21571)
                     /locus_tag="ACG8PO_001439"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0206758.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(21586..23307)
                     /locus_tag="ACG8PO_001440"
     CDS             complement(21586..23307)
                     /locus_tag="ACG8PO_001440"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0206759.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            23407..24168
                     /locus_tag="ACG8PO_001441"
     CDS             23407..24168
                     /locus_tag="ACG8PO_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFQ0206760.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
BASE COUNT         7697 a         4505 c         5202 g         7301 t
ORIGIN      
        1 aattaaacgg gaagtagctc agcttggtag agcacttggt ttgggaccaa ggggtcgcag
       61 gttcgaatcc tgtcttcccg ataattataa atttaaacac ggggccttag ctcagctggg
      121 agagcgcctg ctttgcacgc aggaggtcag cggttcgatc ccgctaggct ccatttcatt
      181 taaataatga atatcttttt cgcggtgtag ctcagctggc tagagcgtcc ggttcatacc
      241 cgggaggtcg ggggttcgat cccctccgcc gcgattttta agagcctgga cctttagctc
      301 agttggttag agcagacggc tcataaccgt ccggtcgtag gttcgagtcc tacaaggtcc
      361 attgtggcgt tgtaagtttc atatggagga ttacccaagt ccggctgaag ggaacggtct
      421 tgaaaaccgt caggcgggta aaaccgtgca agggttcgaa tcccttatcc tcctttttca
      481 tattatcgcg ggatggagca gtctggtagc tcgtcgggct cataacccga aggtcgtagg
      541 ttcaaatcct gctcccgcaa ttaaaaacta aaaacactgg ttccgtggtg taggggttaa
      601 catgcctgcc tgtcacgcag gagatcgcgg gttcgattcc cgtcggaacc gccatgcggg
      661 tgtagtttag tggtaaaacc acagccttcc aagctgttgt cgcgagttcg attctcgtca
      721 cccgctttta gttttttatg ggcctatagc tcagctggtt agagcgcacg cctgataagc
      781 gtgaggtcga tggttcgagt ccatttaggc ccattagtac aaaatcttat tggttttggg
      841 gaagtactca agtggctgaa gaggcgcccc tgctaagggt gtaggtcggg aaaccggcgc
      901 gagggttcaa atccctcctt ctccgtttca atattttgtt ttacctggcc cgttggtcaa
      961 gcggttaaga caccgccctt tcacggcggt aacacgggtt cgaatcccgt acgggtcatt
     1021 aatttaatat agttttacat ggaggattac ccaagtccgg ctgaagggaa cggtcttgaa
     1081 aaccgtcagg cgggtaaaac cgtgcaaggg ttcgaatccc ttatcctcct ttttcatatt
     1141 atcgcgggat ggagcagtct ggtagctcgt cgggctcata acccgaaggt cgtaggttca
     1201 aatcctgctc ccgcaattaa aaactaaaaa cattggttcc gtggtgtagg ggttaacatg
     1261 cctgcctgtc acgcaggaga tcgcgggttc gattcccgtc ggaaccgttt ggctcggtag
     1321 ctcagttggt agagcaatgg attgaagctc catgtgtcgg cagttcgatt ctgtcccgcg
     1381 ccattatgcg ggtgtagttt agtggtaaaa ccacagcctt ccaagctgtt gtcgcgagtt
     1441 cgattctcgt cacccgcttt tagtttttat gggcctatag ctcagctggt tagagcgcac
     1501 gcctgataag cgtgaggtcg atggttcgag tccatttagg cccatttatt ttattggccc
     1561 gttggtcaag cggttaagac accgcccttt cacggcggta acacgggttc gaatcccgta
     1621 cgggtcatgg aagatacttt ttgagtatct ttttcttttt atctgaaaga atgattttaa
     1681 aaagcttagc agagttatct ttatggatga ttgtgctaag cttttttgga cagttctaat
     1741 gtaaaatatt tttatatttt ttaagcaaaa aaatgaaaaa atacgagcag aaaactgttt
     1801 tatctgctat acttggttcg ttgcaagttc ccgaaaggat ccgcaggacg gagatgcctt
     1861 gtaaccgaat ttaactaggg ggaatcatta taatggtaga aagacactta tttacatcag
     1921 aatctgtttc tgaaggacat ccagataaaa ttgctgacca aatcagtgat gcaatcttgg
     1981 atgcaatttt aaaacaagat ccaacagcac gagtagcgtg tgaaacgtct gtaacaactg
     2041 gtcttgtttt agtatttgga gaaatatcta cgacggcata tgtagatatt caaaaagtcg
     2101 tacgggaaac aataaaagaa attggttata cacgtgcaaa attcggattt gatggagata
     2161 cagcagctgt attggttgcg attgatgaac aatctcctga tattgctcaa ggagttgatg
     2221 aagcacttga gatccgggat gaagataaaa aagacgtatt agatgagatt ggtgctggtg
     2281 accaaggttt aatgtttggg tttgctgtag atgaaacacc agaattgatg cctttaccaa
     2341 tcgctttgag tcatcgtctg gtacgacgct tggcagattt gcgtaaatca aatgaattaa
     2401 cgtatttacg tccagatgca aaatctcaag taacggttga atatgatgat caaggacaac
     2461 cggaacgcgt agatacaatc gttatttcaa cacagcatga tgatgcagta gataatgaaa
     2521 caattcgtca tgatgtcatc gaaaaagtag tgaaggaagt tattccagct gaattattag
     2581 atgatcaaac gaaatattat atcaatccga ctggccgatt tgtcattggt ggtcctcaag
     2641 gggatgccgg attaacagga agaaaaatca ttgttgatac gtatggcggt tatgctcgtc
     2701 atggtggcgg tgctttttct ggtaaagatg cgacaaaagt tgaccgttct gccagctatg
     2761 ctgcacgtta tattgctaaa aacattgttg cagcagggct tgctcgcaaa gcagaagtac
     2821 agctagctta tgcgattggt gttgctcaac ctgtttcgat ctcaatcaat actttcggta
     2881 ctggaacggt tccggaagaa gaactgattg ctgcagtgag agaaaacttc gatcttcggc
     2941 cagcaggaat catcgagatg cttgatttgc gtcgtccgat ttacaaacaa acagctgctt
     3001 acggtcattt tggtcgtaca gatgtagatt tgccttggga acatacggac aaagtagatg
     3061 cgttgaaagc aagtttagca aaataagtta tattcagctg gaacgatcgc caagtggtgg
     3121 ttgttccagc tttttttatg gcagttaacc tcttgaaaaa gatagtaatt atttatctat
     3181 ttttagatcc tgtcataatg aaagggagga ataagatgaa aaaagaaaca aatgttactt
     3241 tggtcacagt gagtattttc attgcaacgt tcatgacggc tattgaaggt acaatcgtaa
     3301 caaccgctat gccaacgatt gtcggttcat tacatggaat ggaaatcatg aactgggtat
     3361 tttcgattta tttgctgaca aacgctatgc ttactccgat ttacggaaaa cttgcggata
     3421 aagtgggacg gaaaccaatc tttatttttg ggacattatt gtttattatc ggttcgttat
     3481 tttgcggttt atctcaaagc atgattgggt tgattgctgc tcgtgcgatt caagggatgg
     3541 gtgctggggc aatgatgcca gtagctttga cgatcatcgg cgatctttat tctattgaaa
     3601 agcgagcaaa agttttaggt ttgaatagtt cagcctgggg gattgccagt gtttttggac
     3661 cgcttgcggg aggattcatc gttgaaacga tcagctggca ctggatcttt tttatcaatg
     3721 tgccgatcgg aattctatta attattttga tttggctata tttaaatgaa ccaaaaatca
     3781 tacatgagtc taaaccaatg gatatttttg gtagttttag tttgatggcc atgcttgtct
     3841 cattgctgtt agggttccaa ttattaggag acgaaggaat gagtccgctt gtttctgctt
     3901 tgctgctagg tagtatcgta atgttaggta tttttgtttt tgccgaaaaa agagctaaag
     3961 atcctgtcat catgatgcat ttattcaaaa atccactatt tgttgtggtc aacttggctg
     4021 cagcattggc tagtggtttt ttgatgggaa tcgatgttta tatccctatg tggatgcaag
     4081 gtgtattagg gctgaatgca gcaattggtg ggctggtatt agcaccatta tcactagtct
     4141 ggatggtcgg ttctttcctt gcaagcaagt ggatgactca aatgaaaact cattgggtct
     4201 tgagaattgg actactaatc gcagctattg ggggcgcttg gttgtatctt atgcctttca
     4261 gaagtaacta tttatggttt tttgctatat caacggtcat cggtataggc ttaggtgtga
     4321 ccatgactac ctcgactgtc agtgcacaaa atagtgtatc tcgtgaccag cttggtgtag
     4381 caacttcttt taatacgtta gttcgaacaa ttggtcaaac agtcatggtt tctatttttg
     4441 gtttattgat aaatggggta actaaacgag aacttgcagc agcaaattta acaaatgatg
     4501 cagatatcat gaataaactg gtgaatccac aaacggctaa atatattgat gcggatattt
     4561 tgttaccatt aagaaaaatt ctttatgatg ggttacattc cgtttatcta gttggcttgt
     4621 tactcgtgat cattgctttg gtcttaacgc ttttcgttca tgaaaaaagt ggtaaaataa
     4681 aacagtagtt tcggttaaga tatgtacgta gccgaatcat tcaaatttaa agttattcaa
     4741 aaataacttc ttactaagga aggcgaaaat atggcattta ttcaagcaaa tatttattcg
     4801 aatgtgttag aaatggaagt caatctaaat gtgatactac cgcaaacaac aaaaaaggtg
     4861 attggcacca gttcagagca agagacaacg gatatacctg ttttatattt acttcatgga
     4921 atgggtggca atcatagtgt atgggaaaga cgtacgtcga ttgaacgtta tgtagcaaac
     4981 tacggattag ctgtcatcat gccatctact gatcttggct ggtacacaga tacagtttat
     5041 gacatgaagt attggacatt cattgcagaa gagctacctg agatttgtca tgaattattc
     5101 ccgcaattaa caagaaaaag agaaaaaaca tatgccgctg gattatcgat gggcggatat
     5161 ggcgcgttaa aactaggcct tgcaaaatca gaaaatttcg cagcagtaat ctctctttca
     5221 ggcgctgttt ctgtaggaaa tcgaatggat gatcttttga tggttcgtaa agcatctttt
     5281 tgggaaggga tctttggacc attggatcaa atcgaaggtt caaaaaatga tccgcaatac
     5341 ttattgaaag aactagtaaa aagcggaaaa ccagttccaa gattttatat ggcttgtggg
     5401 gaatctgatt ttctttatca ggcaaatcag gaaatggtat ctgctatgca ggaacaaggc
     5461 ttggcggtca cctttgaaca aggacctggc gatcataact ggatcttctg ggaccaatgg
     5521 attcaacgtg cattggcgtg gctaatggaa gaaaaataaa aaaagagtct taaaaagata
     5581 gctcctatag agatcacaaa catagtgatc tctataggag cttttttact gctcgttttc
     5641 tcttctttta ttttgttggc ataagccttc aatataggtt tgtgcactcg ttagaaagag
     5701 tgctttttca ttcaattctg catgggactg catactttct ttaaatccct ctaattgatt
     5761 ttcaactaca taattacgca taaatagaac tgtttctttc aaaaattcat ttccgtccat
     5821 aattttttgt cgaagctgct gttcatgctg ctgatccatt aactgtccaa tcaataaatt
     5881 gtggagagag gcaatggcct gatgactttg ggtgactgaa aatccttcac gaacaagtaa
     5941 ttggatcatt tgctccaaat gttctaatcg actcggaaaa gcgggcacag tattgatttc
     6001 taattctgcc ccacaaggga aacgtgtata aaggtcatag taattttcca tatactgcag
     6061 caactgttca ttccaaggca aagttggatc tggaagcgct aattcttctt ccattgtttc
     6121 agccattatt tgcaagagct gttgtttgtt tttgaaatac caatataaag caggtgcttg
     6181 aacatgaatt ctgtttgcga tgtttcgcat ggaaagctgc gaaagagctt gtgtttcagc
     6241 caggatatca aaggcggcct gaatgatgat tgcttgagaa agctttattt ccattttatc
     6301 aacctcgtgt attgcattat ctttttaaac atggtaaatt gttagtcggt ctaattttac
     6361 agtgttaaat tagtcctgtc aatttaaaca aaggggtgta atcaacgaat gaaattaatc
     6421 ttacgacatg cgaaaaaata taaagtagct gtttttattt ccttgctgtc cgtagcagtc
     6481 atggttgcag cagcattatg gcaacctaag ttactacagc aagtattgga agctattatt
     6541 acagaagata atgatgaaat gaaaacaatt ggtatttacc tgatcagtct ggctttgcta
     6601 ggattagccg caggtgtaac gaacacgatt ttctctgcaa aagtagctca aggcgtaagt
     6661 gcagatatcc gagaagaagc atttcgaaag atccagacat tttcatttgg aaatatcgaa
     6721 cagttctctg ctggaaatct tgttgttcgt ctaacaaatg atatcacgca aatccaaaat
     6781 ttagtgatga tttctttgca atcacttttt cgaattccat tcttatttat tggtgccttc
     6841 attttagcta tgatcacgat gccgcaatta tggtggatca tcgtagcgct tatcgttacg
     6901 gtatttctga ttacggcact ttcatttaca aggatgggca agcacttcat gatcattcaa
     6961 aaactgattg atcgtgtgaa tagcattgca aaagaaaact tactagggat tcgtgtagtg
     7021 aaatcttttg tacaagaaga aaaccaattg aagtcattca gcaaagtaag tgaggactta
     7081 acaaaacaca acatcatagt cggtacatta ttttctgtga tgatcccgtc cttcatgctg
     7141 gctgccaatt tggctgtggt tggggcgatc ttttttgtga gcgatcttgt taaagatgat
     7201 cctacgttaa ttggtggaat tgcttcattc atgaactact tgatgcagat tatgatggca
     7261 atcattattg gcggaatgat gatgatgatg acttcaagag cagccgtatc gctgaagcgg
     7321 attgcggaaa ttttagatgc tgaaccagat cttacttatt tagacgttcc agaacaagag
     7381 ttgacgggtt ctgtcacttt tgaccatgtt tcttttcgtt atcctggtga ggatacagat
     7441 acgcttaaag atatcagctt ttctatcaaa cctggagaaa tggttggtat cgtcggggcg
     7501 actggtgccg ggaagtcaac actagcacaa ttgatccctc gtctatttga tccgacagaa
     7561 ggcaaagttg aagtaggtgg tgtagatctt cgtcaagtaa atgaaaagag tttaagacaa
     7621 acggtttcct ttgttttgca aaaagcaatt ttattctcag gaacgattgc tcaaaacttg
     7681 cgccaaggga aaaagaatgc aacagaaaaa gagatggaac atgcttcttc tattgctcaa
     7741 gcaaaagaat tcatcgaaaa gttatctgat cgttacgaag caccaataga agaacgtagc
     7801 agtaactttt ctggtggtca aaagcaacgt ttatccatta ctcgtggcgt tatcggtaat
     7861 ccaaagatct tgatcctaga tgacagtacg agtgcactgg atgctcgctc tgaaaagctg
     7921 gtccgagaag cactcgatcg agatttgaag ggaacaacga cgattgtcat cgcacaaaaa
     7981 attgcttctg ttgtcaaagc agaccgcatc ttagtattgg atgaaggcag attagtcggc
     8041 gaaggaaccc atgaggaatt ggtagctgga aaccgtgtct atcaggaaat ctttgaaact
     8101 caaaaaggaa cggaggagta agtatgactg atctaataaa agcaagtaaa tttttctatc
     8161 actatttgaa gcggtacaaa ctctcctttt tctttatctt tatcacagtg atcattgcta
     8221 cctatctaca agtaaaggca cctcaataca tcggggaagc ttttcaagaa ttagctaatt
     8281 acgtaggagg cttgatgcaa ggcgtagacg ataaaagcaa atttgtttct gtaatatgga
     8341 aactcttgct gttttatgta ctagctagtg cagccaactt tatctatagt attttgttca
     8401 cacaagtagt agggaagtca acgaaccgaa tgcgtatcgg tctattcaac aaattggaaa
     8461 aattgacgat ccgtttcttt gattctcatc aagacggtga aattttaagt cgttttacca
     8521 gtgacttaga taatattcaa aatagtttga accaagcttt gcttcaagtc atcacgaatg
     8581 ctgttttact tgtcgggatc ttgatcatga tgttccgcca aaatgtagag ttagcttggg
     8641 ctacaattgc ttccactcca gtagccgttt tgatagctgt cttgattatc cgcaaagcac
     8701 ggaaatatgt ggatcttcag caagatgaag tggggaaact aaatggttat atggatgaaa
     8761 aaatcagcgg acaacgtgtc atcatcacaa atggcttaca agaagaaacg attgaaggat
     8821 tcttagagca taatcaaagt gttcgcgaag caaccttcaa aggacaagct tattctggtc
     8881 tactatttcc gatgatgcaa ggaatgtcac tggtcaatac agcgatagtt atctttttcg
     8941 gcggctggtt agcattaaat ggtgatttgg aacgttcggt ggcattaggc ttagttgtga
     9001 cattcgtcca gtattcacaa caatattacc aaccactgat gcagatttca tctggataca
     9061 gcatgatcca attagcgatt acaggggcaa gacgattgaa tgaaatattt gatgaaaaag
     9121 atgaagtcaa accagaaaac tgtaaagcat ttgatggtgt tgaaaaaggt cttgcgttgc
     9181 agcacgtaga ttttggttat tctccagaaa aactagtctt gaaagatgtc tcgatcaatg
     9241 tggacaaagg agaaatggtt gctcttgttg gtccaacagg atcggggaaa acgacgatca
     9301 tgaatctgct aaaccgtttt tacgatgtca atgatggttc agtagtcatc gatggaatag
     9361 atatccgaga ttatgatctt gatgcattac gtagacatgt aggaatcgtt ttgcaagaat
     9421 ctgtattgtt ctccggaacg atcaaagaca atatcacatt cggcaaaccg gacgcttcag
     9481 atgaagaagt catcaatgca gcaaaacaag cgaatattca tgacttcatc atgacgttag
     9541 aaaaaggtta tgatacagag atatccgaag aaaataactt attcagtaca ggtcaaaagc
     9601 agttgatcag tattgctcga acgatcatca caaatccatc tttattgatt ttggatgaag
     9661 caacaagtaa tgtagacacg gtaacagaag caaggatcca aaaagcaatg gatgaagcaa
     9721 tcaaaggaag aaccagcttt gtgattgctc accggctgaa aacaatcttg aatgcagata
     9781 gaatcgttgt cttgcgtgat ggggaagtca ttgaagaagg cagtcatcac gagttattag
     9841 cagaaaatgg attttatgcg gaactttatc ataatcaatt tgtatttgaa taaagaggtt
     9901 gtgaaaaagc tgtcctgcat caagtaataa gaacagccaa tcaaaaacag cttctcatgt
     9961 ttttgatttg gttggactta ttaccgcaga tataagcttt tgagcaccgt gaataaagag
    10021 gttatgaaat caacgctcta acctgagtat tagagaaaaa aacgcaaaac atatcattta
    10081 tgttttgcgt tttttattta tctttttttc tcaatacaat ataaaatagg cggctggttc
    10141 ttttggttga taaaccgata attcagcaca ttatatgttt cctgtggcag tttggaacaa
    10201 aaagaatcaa ccatatccag ttctttttct ccaccttcat gaccatagta gacgacaagg
    10261 atcaatcgtc cgcgaggcac aagtcgtttc aatatttctt ccatagctgt gcgtgtagtc
    10321 tctggcatag tgatgattgc tttatcactt tttgggagat accctagatt gaaaatccct
    10381 gctttgagat tttgttgttc agctatcact tctcctaacg tttcgtggcc ttgtagaaac
    10441 aatgagacac gttcttctaa ctgacgctgt tctagccttt cacgagtagc atcgattgct
    10501 tgttgctgga tatcaaaact atagacatga ccagtttttc cgatgtgttc tgctagaaaa
    10561 accgtatcgt ggccattgcc catcgttgca tctacgacgt gatcccctgg ttgcaagatt
    10621 tcttgcagta atgtatggct gaaatgcaaa gcagtttgca acatcagacg ctcacctttt
    10681 tccgtacatc atattttcct tggaagctat ctctgcgaag aagttcttca tcaattgcat
    10741 tcaagacttc ccattttttc aagctccaca ttggtccaat caaggaatcc cacggcgcat
    10801 cccctgtcaa acgatggatg atgatctctt ttggtatcat ttccagctgg tcgcaaatga
    10861 cattgacata gtcaggacgg ctcattaatt gaaggcgacc ttcatgatag tcccgcaaca
    10921 ttctcgtttt gcgcatcaga tgcatcaaat ggagcttgat tccttggata tctgaatcta
    10981 aaatggtgcg tctgacattt tctttcatca tttctaaact ttcgcctggt aatccgttga
    11041 tcaaatgcgt acaaacacgg atattatgtt tgcgtaattt agccacgcca tctaaataag
    11101 tttgataatc atgtgctcgg ttgatcagtt ttgaagtttc ttcaaaagtt gtttgcaatc
    11161 caagttctac ccataaatac atccgctcgt tcagttcagc aaggtaatca actacttcat
    11221 ctggcagaca atccggtcgt gtaccgactg ataatcctac gacacctggt aaattgacta
    11281 cttgctcaaa tcgttctttg attatttcaa ctggcgcatg ggtgtttgta aaattttgga
    11341 aataaacgat gtattgttgg acatgaggcc atttttgatg catcatgtcg atttctttct
    11401 ggaattggat tggaagaggg tcttcaggtg caacgatcat gtctcccgaa ccagatacac
    11461 tgcaaaaagt acaaccgcca tgggcaacag tcccatctcg attcggacag tcaaatcctc
    11521 catcgatcgg caccttgaaa attttcccgc caaactcatt tcgtaaagcg taattccaag
    11581 aatgatatct tttatttcct tctgaataag gaaaagtcat taaaagtcct tctttctgta
    11641 agagttatct atttgttttc ttttttctct atacactgga taagtcaggc acagccaaga
    11701 aagactgagt aagtaaccgc cgatgatatc agtaggaaaa tgaacgccca gataaatgcg
    11761 gcttatcccg acacttacta taataaacat catcagcaat tgaagcaatg tgcgtaaggt
    11821 tcttttttga acaaaagctg gaagcagaaa tagtaaagaa ccgtaaagaa tcatactgcc
    11881 tgctgcatgt ccactaggga aactgtaact tgtttcaact actaagtgcg ccaatgaagg
    11941 acgctctcgc atgaaaaata gtttgatcac cgggttgatg attccagcaa ttacaataaa
    12001 tccagaaacg aaccaaaatg tttctgccct ttttttctta taccacatcc ataaagccgc
    12061 acaagcaaat aaaatgatga cggtaagagg attaccgaat ttagtcataa agagttggta
    12121 gctgttcaga tcaggataag aagctctgac aatgcctgta aaccactgat cgaaaccaga
    12181 aagccacgac agatggaact ttacgatcca gcccaaaatg aaaaacagga caagaaagaa
    12241 gctaccagcc acctgaaatg atgttttata tttcatacaa ttcctccttt gaagcaatga
    12301 cttattatag catttttggt ccagcgaaaa aaacggtaaa gagataaaac caatgtatga
    12361 ttcctctttg aataaccaat attcttataa aaaaaataac tgtaatcgga aagacatttt
    12421 ttggtaatgc aaatgaaatg ttagggaaat aaaatcagtg tttttcgaaa tccttttaaa
    12481 caagaacgta gacgtaggaa gaacttgaaa atgaacagaa gttgttcatt acggtaaatt
    12541 acaatatgat tacaaaaaac aacaaagctc ttaaataact tttaataaat attaagagaa
    12601 gttataatta ttaataatag ataaattgaa gttggaggat agaagatgga aaacattgca
    12661 agaaaagaac ggagacgttt agaacagacc aaacgttttc gaaaagcaaa acgaggggca
    12721 gcaatcgtgg gaactgcgat ggtaggctgt tctgtcgctg ccccattagt acagccggtt
    12781 cctgttcaag cggatgaaac acctgttcag ttcggcgcaa gaatcaatac agcggcattt
    12841 atcacagaaa ttgctactta tgctcaacca atcgctcaag ccaacgatct atatgcatca
    12901 gtgatgatcg cacaagcagt tgtagaaagc ggttggggca gtagtgcgtt atctcaagca
    12961 ccttattata atctttttgg gataaaaggc agctatcaag gacaaaccgt ctacatggat
    13021 acgcttgaat ttttaaataa tcaatgggta aataaaaagg agcctttccg acaatatcct
    13081 tcttttgctg aatctttcaa tgacaatgcc tatgtactta gaaatacttc atttggcaac
    13141 ggttattact atgccggtac ttggaagagc aacacgaaat cctatacaga tgcgacagct
    13201 tgtttgacag ggcgttatgc gactgatcct ggttacgcag gcaaattaaa taatatcatc
    13261 gcaacttacg gattgaccca atatgataca ccagctagcg gaaacgcagg cggtggagcg
    13321 actacaggtg gcaataatac tggaagtgca ggtagtactg gcggttcaag caattctggt
    13381 aatacaaact caggttcagc aacttcgacc acttatactg tccaatctgg tgattctgtt
    13441 tggggaatct caaataaatt cggtatcaca atggatcaat tgatccaatg gaacaacatc
    13501 caaaacaatt tcatttatcc aggacaaaaa ctgacaatca aaggtagcca ggaaaatggc
    13561 tcttcgacga ataattccgg taacaatacc aattcttcag gaaatgctgg tacatcaaac
    13621 ggaggacaaa caactggtgc gaaatacact gttcaatctg gtgattctgt ttggaagatc
    13681 tcaaatgatc acggaattac gatggctcaa ttgattgaat ggaacaatat caagaacaat
    13741 ttcgtttatc caggacaaca gctaatcgta tcaaatggcg gttcttctaa tgctggagca
    13801 aatactggct caaatactgg gaatacaagt ggctctagtt cgtccagctc aagttcttct
    13861 aatacagctt caggaacgaa atatacagtt aaagccggag actctgtttg gagcgtctca
    13921 aataaatacg gaattacgat ggctcagcta atccaatgga acaatatcca aaataacttt
    13981 atctacccag gacaacaact gactgtttca aacagtggat caacaaacag cacgtcaggt
    14041 aatacaggtt cgtcaaccaa tacaaataac agcagttcag ttaatactgg cagcagttcg
    14101 tctcaaggca cttataccgt gaaagcaggt gaatctgttt ggagcgtggc aaataaaaac
    14161 ggcatcacga tggatcagtt aatcgaatgg aataatatca aaaacaactt catttatcca
    14221 ggacagcaat tgatcgttaa aggtggaact tcggcaaata caaatacagg ttcaactact
    14281 tctgcagcga aaccaaatac accgaatacg tctgctacga caagtacatc atccactggt
    14341 aatacgatgt ataccgtgaa agcgggcgag tcagtttgga gcgtagcgaa caaacatcat
    14401 atcacgatgg atcagttaat cgaatggaat aatatcaaaa ataatttcat ctatccagga
    14461 cagcaagtga ttgtgaagaa aggttcatct caatcaacaa accaacaatc agttactggt
    14521 tcgaaaacct atactgtgaa atctggagaa tctgtttgga gtgttgctga cagtcatgga
    14581 attacaatgg ctcaactaat cgaatggaac aatattaaga acaactttat ttatccaggt
    14641 caaacgttga ttgtagctaa ataaaatagc tgttcgaaga aataacgatt gaattccaca
    14701 aaaatatttg ttactataag gctacttaaa tatgacaaca cttgaaaagg aagtagtaaa
    14761 aagactgaag tttcagagag cgcatggctg gtgagaatgt gcacggatgc ttttgaactc
    14821 gcctttgagt tgcccttgct gaatacccga gtaagcaagc ggcggtgacg gccgttacaa
    14881 cgttcgaggc cttttcacta aggtaaaatt aggtggtacc gcgtagatta cgtcctataa
    14941 aaagagcaat ctttttatag gattttttta tttcttttgt gaagtttgag aaaacaaagc
    15001 aaaaggaaat aaaaataaac agattctgcc ggttttttcg ttatatttat aatagaaaca
    15061 agtgttttca atagaacagg aggacagcaa atgagttaca atcataaaga aattgagaaa
    15121 aaatggcaaa aatactgggc aaaaacgaat actttcaaca cccatgatga tcctgaaaag
    15181 ccaaaatttt acgcactgga tatgtttccc tatccatccg gtcaagggct acatgtaggg
    15241 catcctgaag gttatacggc aacggatatc ctttcacgtg tgaaaagaag tcaaggatac
    15301 aacgtgctgc atccaatggg gtgggatgca ttcggtctgc ctgctgaaca atatgcgcta
    15361 gatacaggaa acgatccagc tgagtttacc caaaaaaata tcgaaacatt ccgacgccaa
    15421 atcaattctt taggatttag ttatgactgg aatcgcgaag tgaatacaac tgatccggag
    15481 tattataaat ggacacaatg gatctttacg aagctttatg aaaaaggtct agcttatgaa
    15541 gcagaagtag cggtaaactg ggtacctgaa ttaggaacag ttatttcaaa tgaagaagtg
    15601 atcgatggga aaagtgaacg tggtggctat gatgtagtca gaaaaccaat gcgccaatgg
    15661 atgctgaaaa tcactgctta tgctgatcgc ttattggatg atctagaatt agttgattgg
    15721 ccagaaagca ttaaagaaat gcaaagaaat tggatcggtc gttcagtagg agcaaatatt
    15781 gaatttaaag tggctggtac ggataaatcc tataccgtat ttacgacacg accagatact
    15841 ctattcggtg caacttactc ggttcttgca cctgaactag atcttgttag agaaattacg
    15901 acccctgaac aaaaagctgc tgtagaagct tatatcgaag agacagctaa aaaatcagat
    15961 ctaaagagaa cggatcttgc aaaagaaaaa acaggagtat ttactggtgc ttatgcaatc
    16021 aatccagtta atggcaagga aatcccaatc tggatcgccg attatgtatt atcttcatac
    16081 ggcacaggag ccatcatggc tgttccagca catgatgaac gtgactttga attcgctcaa
    16141 actttcggac tagaaattct tccagttatc gaaggtggag atgttgaaaa agctgcttac
    16201 acagaagatg gtacgcacat caactctgaa tttctagacg gcatgaacaa acaagaagca
    16261 atcgataaaa tgaatgaatg gctagaagaa aatggcgtag ggaaaaaaga agtcagctat
    16321 cgcttacgtg actggctatt ctcccgtcaa cgttactggg gtgaaccaat cccaatcatc
    16381 cattgggagg acggaacagt cacagctgtc ccagaagaag agctaccatt acggttgcct
    16441 aagacaaaaa acatcaagcc aagcggtact ggagaatctc cattagcaaa tatcgaggag
    16501 tgggtcaatg tcgttgatcc tgtaacaggt aaaaaaggcc gtcgcgaaac gaatacaatg
    16561 ccacaatggg caggaagctc atggtattac ctgcgttata tcgaccctca taacaaaaaa
    16621 gaactagcag attatgaaaa gttgaagcgt tggctaccag ttgacatcta cattggtgga
    16681 gcagaacatg cggtacttca tttactatat gcacgattct ggcataaatt cttatacgat
    16741 atcggtgttg ttcctacaaa agaaccattc caaaaactat acaatcaagg aatgatctta
    16801 ggcgaaaaca acgaaaaaat gtcaaaatct cgtggtaatg tagtcaatcc tgatgatgtg
    16861 gtcgaaactt acggtgcaga tacactgcgc atgtacgaaa tgttcatggg gccattagat
    16921 gcttcgattg cttggagcga aaatggctta gaaggaagcc gtaaattctt ggatcgtgta
    16981 tggcgtttga tcgttgatga gaacaataaa atgcgtgacc gtatcactac attgaatgat
    17041 ggcaagcttg ataaagtgta tcatcaaaca gtgaaaaaag tgacagaaga ctatgaaaat
    17101 cttcatttca atacagcaat ctcccaattg atggtcttta tcaatgaagc ctataaagtg
    17161 gacgctctgc cttatgaata tatcgaagga tttgttcaac tactcgcacc gattgctcct
    17221 cacatgggtg aagaactatg ggcaatcctt ggaaatgatg gtggtatttc ttacgcacca
    17281 tggccaacat acgatgaagc agcacttgtt gaagacgaag tagaagttgt tttccaagta
    17341 aacggtaaag ttcgtgcgaa aagcaatgtt ccccgagact taggaaaaga tgagctagaa
    17401 aaagcagcat tagccaatga aatagtgcaa gaatatattg aaggcaaaac agtcagaaaa
    17461 gttattgcgg taccaaacaa gttagtcaat atcgttgcta attaattggc tctttgtcaa
    17521 taaggactga tgagttgtgc aaaatcaaat ctgggccaga atgaacttca ttctgtctca
    17581 gattttttgt tatttcaaat tgattaattg attgatcaaa aaggttttga ttttcatgtt
    17641 ctcaaacacg ttcaatgtct ttttcttctg aatggtcttg taagagatct ctgtaatcat
    17701 caaatttttg tctattaatc ttagtatttt ttgatgtata aactacctga aattgagagt
    17761 acataccaaa ttcataaatt ttttattagt gggactagtg attgtatgga ttttgacgca
    17821 taatgaatga agtatgagaa aaggagagac aaatggatca agaactattt attatcgaag
    17881 tattatcagc taaacaaaaa attatcgaga aagcgaaaaa aagagcgaaa agaataatgt
    17941 tcaagctttt aagcttagta tttttctctg gtttgctttt tttcggagct gtatcgttcc
    18001 tatatcgcca tatgagtgca gtagtccgtt cagctgaagg gatgcaaaaa ttaaataatt
    18061 tgtatcactt ttcgtgggga gttggcggaa taacaattgt tgtgcttatt gtttttcttc
    18121 tacttatttg gctccgttac catcaattgt cgcgtcattt cgatcaatgg cttagtcaaa
    18181 gagcacaaca gtttttacat aataaaccaa cttctcaaga tgatgactat ctttattttg
    18241 agaagacttc gacgagtcaa gcttttcgga tacaaaaaag tgattgccag ttattgctga
    18301 gatcacttga tgatttacct atctacttag gcgaaagcaa atcaaaattc ggaatgaaca
    18361 cattccatat ttttttacta gaaaacgaac ccagatggga aaaaagccag tcttcgcctg
    18421 gtggatacct gacaactaag atgagtacat tagtcactgg ttttttcctc attcttttag
    18481 ctggaacaat ctattatggg agatacactc agccaaaaat gtcttctcat ataaatgctg
    18541 cttctcacat gacaatgaac tcaactaacg atacgcatat acaaaagaca aatgaagggt
    18601 tgttagctga tcctaagcaa aaggtaaatt tgacaacaca aaagataaat gatctcaaac
    18661 ttgatacaga gacgcactct ctctatatga caacagatag tggtgctaat tgggtatttg
    18721 tgccgattga ttcgcaatgg ttgcgttttg gcgaatacac cctgacaacg gggctgattc
    18781 ctgaaggtta ttggatggac aacacatatg atatttccgc cgatttctca tggtttatct
    18841 attcttctgg ggatgatgta cgtctgctga cttcaagaga taatggtaaa acatggcaaa
    18901 cgtctattat cacccaatca gttcagccga tacgttatcg aaaagcggta ttttatggca
    18961 acgatggtct atttatctgt acatcagcca gtgggatgtc tgcagaaact ttgtacattt
    19021 acactactat ggatggcgga atcacttgga taaaaagtgg aagtacaatg atcaatcagc
    19081 cagtacaaaa tgtcagcttc gttacaactt ctttaggttt tgtttcgaca cgtgaaagtt
    19141 tgttttatac aaacaacgga ggacgctcat tcaaagaatc agttctgtcg attccggaaa
    19201 actatcagac aggggggata gatctttttc aatcaccaaa tgaagttact caaataagcg
    19261 cgaatacgtt agagacaaaa ttttatttgt tgaaaaaagg accaatcgat gcagggaaaa
    19321 tgtttgcctg cctcttccgt tcaactgata acggggaaac atggcaattc gttcagcaac
    19381 tatcgcaagt agaacaagga gattaaagaa gagagattgt gagcctgacg ttcagcaccg
    19441 agtcacaaga aacaaattca aaaaacagct tctcatattt gttgaatagt gtaactcgtg
    19501 attcggagtt gacaagagca acaagaagcg attttgaagt atagcttctc gtattttaca
    19561 agatcgtgtc ttattgccga taggttgtct cttgaacacc gtttattcgt aaaaagggat
    19621 tgtgacaagt cttttgtcac tacttaccga acgaaactga aggagctatc caaaaagtat
    19681 cattagtatt attctttgtg tttctagtgg caccactagt acatttaaaa aagctatcga
    19741 tcatggacta atcatacatg atcgatagct ttttagataa tgtttgttac gctatctgaa
    19801 attgactatt ataaagactg gcataaagcg ttggtgattg gagtagttca gaatgtgttc
    19861 ctttttcgac gatatcgcca ttcttcatta caaggatcat gtctgcattt tcaatcgttg
    19921 acaaacggtg tgcaatcaca aaacttgtcc tatcttttgt cacagcttcc attgcttgct
    19981 gaatcaatgc ttctgttcgt gtatctacgc tagaagttgc ttcatctaaa ataacgacag
    20041 gaggattcgc tagaatgact cgagcaattg tcaataattg ttgctgtcct tgagataacg
    20101 tgccgttttc gctagaaatg atcgtatcat atccgtctgg cagtgttcga ataaagtgat
    20161 cacattgtgc gatttcagcc gcttcgataa tttgttctct tgtggcatcc attttcccgt
    20221 aggcgatatt atcagctacc gttccttcaa acagccaggt attttgtaaa accatcccaa
    20281 aagaacgacg tagttctcca cgtgaaagct tggtaatatc aagaccgtcg aattcgatct
    20341 ttcctttatt cagttcataa aaacgcatca gcagattgac taaagtcgtt tttcctgcac
    20401 cagttggacc aacgatcgct accgtttgtt ttggctttac tgaaaaatcg acttgattca
    20461 tcaatatttt atctgatgtg taaccaaact gtacattttt gaaagctatt tttcctttcg
    20521 gttgttctaa aagtcttgca tctggaaggt ctggaatttc atctgtttca tctaatatct
    20581 caaatatcct atcgattgct gccattgcgg attggatcga gttgatcaca taagaagcgg
    20641 tagaaattgg ttcagaaatc tgattgatgt actgaagata agcttgtaag aagccgatcg
    20701 tcacaccacc tgatagaaca agcatagcac cgaataccgc acttatgatg aaggcgagtt
    20761 gattaatgaa acgaatggcc ggatagatag cacagttcat aaattgagca cgtttaaaag
    20821 cgttgtattg ctttttattt acttcgtgta tagcagcttc tgcatgagct tgctgattga
    20881 atgttttgac cactagatta ccagctaaga tttcttccat tcgattattt aaaacaccca
    20941 gttcatactg attctgttca gaaatctgtt tgtttttgtt cgcaatctta gttgtaacaa
    21001 ttgaactgcc aacgatcagc aataagacga gcaaagtaag cttgctttcg atataaaaca
    21061 gcatcagacc agcagcagct attgttacga cagaagtaaa aaattgattg atacctgtca
    21121 ataacacttg agaaagttga ttgagaccgg tagttgtccg actaatgatg tcacctactt
    21181 gatgattgtc aaaaaacgac atcggtaatg ttttgaattt ttttgtgatt tcttttcgca
    21241 ccctcaatgt gatcttttcg cttaacgctg ccattgtccg ttcctgaata aatgaagtga
    21301 tgctgcttaa gactgaacag ccgatcagca ataagacagg gaacaacaat gcttgtttga
    21361 tatcactgaa agaaagctgt ccaatacctg tttttcttat ttctgataag agatcatcga
    21421 tcccgattcc catgatcaag ggcatggcta cgactaatag gttcccgaat aaactgcata
    21481 gaaacaatcc gtaaaaatat ttatgttcag gtttgatcat tttccaaaaa cgtatcaaag
    21541 cacgcgtgtt caacgttttt ttcttttcca tcgtatccct tctttctata ttccttgtga
    21601 ttttgcaaac tcctgataag tttggcaatt agacagtagt tcttcatgag taccttgtcc
    21661 aacgattttt ccttcatcta aaacaatgat gttatctgct tgttggattg tactgaggcg
    21721 ttgagcaaca atcacgagag ttttctccga catgtgttta tgaagggctg ctcttaatgc
    21781 tgcatctgtt ttatagtcta aagcggagaa actatcatcg aatacataga tctctgctgg
    21841 tttgatcaac gctcgagcaa tacatatccg ctgtttctgg ccccctgaat aattagaacc
    21901 gccttgagca acaaagctgt ccaatccgtc aggaagggtg tccactagtt cttctaattg
    21961 agcgatcttg atcgctttag acaactcttc tgttgaggca tattcatttc ccatctgcaa
    22021 gttgcttaaa attgtcccgc tgaataagaa agccttctga gggacataac tgatgtggga
    22081 gcggatctgt tcttgagtca tttgagtaag aggaatacca ccaagggtta cttttcctat
    22141 cgtagggtca ctaagtctca agagaagttt cgccaaacta ctttttccag atccggtccc
    22201 gccaacgatc gctgttgttt ttcctttagg tattgtgaaa tcaatatctt ctagaactgg
    22261 atcagagatt tcagtatatt taaatgtgac atgttcaaaa gcagctgcat ttgtctgatc
    22321 aaaggttggt tcagctactg ttttgatcgt ctcttcctca atttcgatct ctgcttgcag
    22381 tatttcttct atccgtcgta atgaggcaaa cgatttaggg ataatgacca ataccatagc
    22441 tgcgatcatc aagtaagcaa gtgtcagcat agagtattcg actacagcag tcacgacacc
    22501 gatttgtaat gctccttctg ctacaagatt tccgccaaac cacaaaatag aagcataaac
    22561 gattcccatg agcaaaaaag caattggagt gacccaagca aatattcggt tcactttgat
    22621 catttgatta gcatagtcag caaagctctt attcgttcgc ttttcttcat atgattgatt
    22681 atcaaaggca cggatcatat taatcccagt aaagaattct ctcaatgtga tggtgatccg
    22741 atccattttt ggctggatac tcaaagataa aggtgcccct cgtttcatca gtcgataaac
    22801 aatccataca taaaaaacaa cagagatcaa tgtaacccat gttagtaata gagaataacg
    22861 aaacatcatg agaatggtga aaacgcaaat aaccggtgct ggtaagatca tttggcaaaa
    22921 taaaacaatc atttgctgga cattatcgac atcattcgtc attcttgtca aaagagatcc
    22981 agaactaaaa cgactgatat ccttcattga cagctgttgg aatttacgga aatatttctc
    23041 ccgcgtttgg aatccaaacc tagcggaagc caatgctgaa aagtaactag aggcaactgc
    23101 agcgattgtt cctagaaacg ctacaagcag catttcaact ccaatttttt ttataactga
    23161 ggcttcgcca cttgctatac ctgtgtcgat cagctttgct actaattttg gtacacctaa
    23221 tgtaccggcg atttctaaac agatcatcac aaaggtaagg agagcgagtc ctttgttttt
    23281 ctgtaagaat gtttttaaga tagacatatt attcctcctt ttttcctgat acttttcgat
    23341 tctatacctt caagtaactt gaaggtcaat agaaaaaaga ataaactgaa aaagaggtga
    23401 gaaaaaatga aaaaaatgta tacgattggc caagtatcgg aactatttga tttgccaaaa
    23461 tcaacgatcc gctattggga tgaacaagga ttaatccgtt caagtagaca agaagagaat
    23521 gattatcgat tgtttgatat cgatgatatt tttatgcttt atgatatcgt tttttataga
    23581 aaattagata taccgatcaa acagatgaag aatttatatg gaaaaacatt gacggagtta
    23641 tatgagacac ttgatgaaac agaaagaaga atccataaag aactggtggt gatgaaacaa
    23701 aaacaaaaag agataaggga gcaaaaaaaa cagttgaaat tgatgatcga tacaaacgag
    23761 gaagaatttc ctgtagaaga gatccctttt gactgtatga tctcaacgga gtttgaagat
    23821 attgtggaaa tcaaaaaatt tctacctaac tattcaagct ttgggatgat gagtatgcca
    23881 gcttccagtt cacagacgat gtatggattt tttatcgacc ctagtgaagt acatttattt
    23941 actcaagacg tgatttggga gaaaaaagat acggctgttt atcgtcgatt cttattgaaa
    24001 tccgagatga accatgcgga acgtaataat attttagaaa tcagagaaag aatgtatgaa
    24061 aaaggatgga aaacaggaga agtgatcggc cagtatctat tgacgaatac ggatgaaaac
    24121 aatatcagaa ctgaatatta tcatgcatgg atagaaatga aaaaatagga aatatgtgtt
    24181 taagataact cgaaaatgat ggttgtttta gttataaaga aaaattttct ggttcgatta
    24241 cgggtttaat gtgggggatt attagtcgtg gatagataga attttagttt tttagaggaa
    24301 atatgtcttt aaaaatggaa tggttgcaat tcttagcgat atatacgatg taattgtcct
    24361 cttaaaaaag tatggaaaaa taattgtatt gggtgttatg tataattcat ggtgaactga
    24421 gactaatgag aagaaaaatt catattgtta tgagtgagat tgtatcagat gagaatgata
    24481 aattaaatag gtgcataaaa gcattacact taaaatacat tgttgcttac attattacaa
    24541 tatgttaata aggcaaaaat aaatagatgg attgaataaa catatttttt aatcaaatag
    24601 tccctactgt gactttcctc agctatcggt gatactaccg atagcatttt tttgcttata
    24661 aaataattat aagtatttag agatttttct tctaacattc ttcaa
//
LOCUS       DAZKKW010000037        24632 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.037, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000037 DAZKKW010000000
VERSION     DAZKKW010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24632
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.037"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            89..1348
                     /gene="lysA"
                     /locus_tag="ACG8PO_001442"
     CDS             89..1348
                     /gene="lysA"
                     /locus_tag="ACG8PO_001442"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFQ0206761.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            1335..2135
                     /gene="dapF"
                     /locus_tag="ACG8PO_001443"
     CDS             1335..2135
                     /gene="dapF"
                     /locus_tag="ACG8PO_001443"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFQ0206762.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            2138..3019
                     /gene="dapA"
                     /locus_tag="ACG8PO_001444"
     CDS             2138..3019
                     /gene="dapA"
                     /locus_tag="ACG8PO_001444"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFQ0206763.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            3016..3732
                     /gene="dapB"
                     /locus_tag="ACG8PO_001445"
     CDS             3016..3732
                     /gene="dapB"
                     /locus_tag="ACG8PO_001445"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFQ0206764.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            3738..4433
                     /gene="dapD"
                     /locus_tag="ACG8PO_001446"
     CDS             3738..4433
                     /gene="dapD"
                     /locus_tag="ACG8PO_001446"
                     /EC_number="2.3.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047200 - tetrahydrodipicolinate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HFQ0206765.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            4449..5450
                     /locus_tag="ACG8PO_001447"
     CDS             4449..5450
                     /locus_tag="ACG8PO_001447"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFQ0206766.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            5447..6808
                     /locus_tag="ACG8PO_001448"
     CDS             5447..6808
                     /locus_tag="ACG8PO_001448"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HFQ0206767.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            6768..7919
                     /locus_tag="ACG8PO_001449"
     CDS             6768..7919
                     /locus_tag="ACG8PO_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFQ0206768.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            7894..9063
                     /locus_tag="ACG8PO_001450"
     CDS             7894..9063
                     /locus_tag="ACG8PO_001450"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFQ0206769.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            9238..10617
                     /locus_tag="ACG8PO_001451"
     CDS             9238..10617
                     /locus_tag="ACG8PO_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFQ0206770.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            10898..11695
                     /locus_tag="ACG8PO_001452"
                     /pseudo
     CDS             10898..11695
                     /locus_tag="ACG8PO_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            11884..12552
                     /locus_tag="ACG8PO_001453"
     CDS             11884..12552
                     /locus_tag="ACG8PO_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HFQ0206771.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(12752..13072)
                     /locus_tag="ACG8PO_001454"
     CDS             complement(12752..13072)
                     /locus_tag="ACG8PO_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0206772.1"
                     /translation="MGDCFCDGRTSQRYQKNEEEARKQPLPKQQMTLQEIDSILTEAF
                     LSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYFMIDHQPILWEDVRYIELKETKK
                     WFVT"
     gene            complement(13109..14491)
                     /locus_tag="ACG8PO_001455"
     CDS             complement(13109..14491)
                     /locus_tag="ACG8PO_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFQ0206773.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            complement(14647..15834)
                     /locus_tag="ACG8PO_001456"
     CDS             complement(14647..15834)
                     /locus_tag="ACG8PO_001456"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HFQ0206774.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            complement(15841..16872)
                     /locus_tag="ACG8PO_001457"
     CDS             complement(15841..16872)
                     /locus_tag="ACG8PO_001457"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFQ0206775.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            complement(16888..18342)
                     /locus_tag="ACG8PO_001458"
     CDS             complement(16888..18342)
                     /locus_tag="ACG8PO_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015486 -
                     glycoside-pentoside-hexuronide:cation symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide (GPH):cation
                     symporter"
                     /protein_id="HFQ0206776.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANVATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMEDELHDAVVPSH"
     gene            18517..19422
                     /locus_tag="ACG8PO_001459"
     CDS             18517..19422
                     /locus_tag="ACG8PO_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HFQ0206777.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            19513..20190
                     /locus_tag="ACG8PO_001460"
     CDS             19513..20190
                     /locus_tag="ACG8PO_001460"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFQ0206778.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            20187..20357
                     /locus_tag="ACG8PO_001461"
     CDS             20187..20357
                     /locus_tag="ACG8PO_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0206779.1"
                     /translation="MTNDWLIQQLDELSEKSEYQDRALLYEIKKLMAEIDERTMQLQG
                     EIDGQSWNHLNW"
     gene            20471..21367
                     /locus_tag="ACG8PO_001462"
     CDS             20471..21367
                     /locus_tag="ACG8PO_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFQ0206780.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(21422..22792)
                     /locus_tag="ACG8PO_001463"
     CDS             complement(21422..22792)
                     /locus_tag="ACG8PO_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFQ0206781.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            23030..23776
                     /locus_tag="ACG8PO_001464"
     CDS             23030..23776
                     /locus_tag="ACG8PO_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFQ0206782.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQSVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            23773..24612
                     /locus_tag="ACG8PO_001465"
     CDS             23773..24612
                     /locus_tag="ACG8PO_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFQ0206783.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
BASE COUNT         7992 a         4542 c         5154 g         6944 t
ORIGIN      
        1 ttgtttaata ttattccaat aaaattcaaa tctttatcaa atgaaattcc aataaaaacg
       61 gaaaaaaagt gaaaagagga atgaaaaaat gacagaattg ataattggcg gagtcacagc
      121 aagcacattg gcagaaaagt ataagacacc tctgtatgtg tatgatgaag aaaagatgag
      181 acaaacgatg ctgtcattca agcgaggctt tgtatcgaaa cgttttgata caaaagtgct
      241 ttacgcctct aaagcattcc aaacgattga gatgctgcat ttagttcgtg aatatggatt
      301 cgggctagat gttgtcagtg gtggggaaat ctataccgct ttgcaagctg attttcctag
      361 agatactatc tacttccatg gtaacaacaa gacgccagag gaactgatct atgcacttga
      421 aaatgatttg ttgcatattg ttgccgataa tttgatggaa gttgaattat tagcagaatt
      481 aagccaacgc tatttaaaaa aaatctgtat catgcttcgg ttgaatgtcg gagtagaagc
      541 acatactcat gaatatatcg tcactgcgca tattgattca aaattcggca tgagctatga
      601 aagtaaagag tgccaacaag cccttcagtt gataaaagaa agcagctatc tagagttaga
      661 aggatttcat gctcatatcg ggtcacaaat ttttgatgtt actgcttggt tagctgaaat
      721 cgacaaattg acgaattact taaccgattt tgaccaacct ctttccttga atcttggcgg
      781 agggtttggg attcgttata cacaaaatga tcagccgatc ccagtcgaag aggcactaaa
      841 aaaactgatt gaatatacgg aagaagcttt ggaaaaacga aaactgacca ttcgtcaatt
      901 actgatcgaa ccgggtagaa gtattgttgg agaagctggt acaacactat acacgattgg
      961 tttcatcaaa aagacaccac ataaaaaata ttactttgta gatggcggga tgacagataa
     1021 tatccgtcca gctctgtatc aggcagaata cgattgtgat ttagcagcaa aactgtcgga
     1081 agaaaaaaca gagaaagtga cgattgcagg aaaaatgtgt gaatcaggcg atgtggtaat
     1141 taaagaagct tatttgccga aagcagaatc gggtgatctt ttagctgttt attccacggg
     1201 agcttatgga tactcgatga gcagcaacta caatcgagcg acacgaccag cagttgtttt
     1261 tgtcaataat ggccaatcaa gactgattgt tcgcagacaa gcattttctg atctactaag
     1321 gggggaagtt tcctatgatc cttcataaat ataatggctg cggaaatgat tttatcttat
     1381 tagattacgc agaagacaca gattatagtc aattagccgc ttctttatgc acaaaagaaa
     1441 aatatgatat agacggattg atcgccgtaa aaatcgatcc gttggaaatg atctattaca
     1501 acaaagatgg cagccgcgcg ccgatgtgcg gaaatggcat tcgctgtttt gctcgatacg
     1561 taaatgatca gaacatgatg aatgaacagg aattcaacgt agagacatta gcaggcacaa
     1621 gaaaaattac ggtgttggat ccctcacctt tttattgcag cgtagatatg ggagtgccag
     1681 atgattctcc ggaaaaaatc aaagctaatc aaacttatcc aataaaaaac caggaaatag
     1741 agatcaatgg tgaaaaagta acgatcacta gtttattttt aggaacgatt cataccgttg
     1801 tttttgttga aaatgcgcgt gaggaagtct ggagaaaaag aggtgaactg atctgtcatc
     1861 atcctttgtt caaagaaaaa accaatgtca actttgtaga agtagtcaat gaacaagaat
     1921 tgatcgtccg tacctatgaa agaggagtag gttggacact tgcttgcggt acaggttgct
     1981 gtgcctctta tgtggttgct aaggaaactg ggaaaatttc agcagatcag gtagttgttc
     2041 acttggaaca aggagacttg atgatctctg gtgaggatgc gatacaaatg gctggcccag
     2101 cagttcatga atggacaaga atattggagg aatgaagatg ataaaaggtt cgatcgtggc
     2161 gctgatcacg ccgatgaatg aagagggatc agtagattat gctggtttgg aaaaattgat
     2221 ccaattccat ttagatgaac aaacagatgg attattagtt ttaggtacaa caggagaatc
     2281 ttctacgctc acacaatctg aggaggagca gatccttcaa ctgacagtaa agaaagtggc
     2341 tggccgtgtg ccggtgattg caggagctgg aacgaataat acgaaagaaa cgattgaaaa
     2401 agcgaagcat tttgcttctt tgggggcaga cgcacttttg gtcatcaccc cttactacaa
     2461 caaaacaagt gatgcaggac tagctgctca ttttacagca attgcagaag cctcaccgat
     2521 tccgcttatt ttgtataatg ttccttctag aacaggcatg tcgattccaa tccatgtatt
     2581 agtaaattta gcagaacatc ctaatatcat tggtttgaaa gaagcctcag gagatatggc
     2641 ttatgtgatg gatgccgctc ggttgattgg agaagaattc ttcctttaca gtggaaatga
     2701 tgatttgatc ctaccagtaa tgagtgtagg aggaagcggt gtaataagtg tatgggcgaa
     2761 tatccaacct aaaatagtcc atgaattagt aaaagatacg caggacggaa gatggcagca
     2821 agcaaaagaa aagcaattaa atgcacttga actgatccat gccttgttca gtgagaccaa
     2881 tcctattcct gtcaaagcag ccatgtcatt attagactta ccttccggac ctctacgcct
     2941 gcctttagtt tctctttcaa aagagaaaaa gaaacaatta gctcagcttt tgttaaaaga
     3001 aaggacggtt gaaaaatgaa aatcggactg attggcagtg gacagatggg aaatagagtc
     3061 gaagaagttg cacaagaaag aggagatacg gtccttcttt tacggacggc acctaaagaa
     3121 gcaactacgc ttgcttttac ggaactgcca gaactgctta ttgatttttc tcatccagat
     3181 aatttagaga aaatcctttc gtatagctta tcatatcaac ttccgttagt cattggcacg
     3241 acaggttata cacaatcaca gttccaagaa ataaaagaac atgccaaaca tgtacctgtg
     3301 atgtatagtg ctaatttttc gttcggcatc atggtgatga atcaactaat caagcaagca
     3361 gctgcaatgt tgcaaggctg gcagatcgaa ctgattgaaa aacatcatag tcgtaaaaaa
     3421 gatgcaccat ctggtactgc aaatatgctg cttcaaacaa tccaagaagt gcaaaagctg
     3481 cagcccgtat acaactggca agggaaaaaa agagaagaaa accaaattgg tgtacacagt
     3541 attcgtgcag gttctttacc tggtgaacat gatgtattgt ttgctgctac tgatgaaata
     3601 tttacgatca aacatgaatc tttttctaat cgaatctttg ctgcaggcgc agtagaagca
     3661 gcggactggt tgaaagatca gtcaccaggt tattacaaat tagaagattt attaatggac
     3721 aaaggggtgt aaaaaacgtg gaagctcaag aaatcatccg ctacattcaa acggcatcta
     3781 aaaagacgcc tgtgaaagtc tatctgaatg taaccgagcc aatcgaattt aaagaaagta
     3841 aagtttttgg tgaaggaagc agcttcgttg tttttggtga ctatgagacg attgctccgg
     3901 ttttagaagc tgaatcagaa aaaatcattg aaatcgaaat cgaaactgct gcaagataca
     3961 gcgcagttcc tttgctagat attaaaaaaa ttaatgctcg tatcgaacca ggtgctatta
     4021 tcagagatca agtgtcaatc ggcaacaatg ccgtgatcat gatgggagcg attatcaata
     4081 ttggtgcagt aatcggtgaa aacacgatga ttgatatggg tgctgtctta ggtggacgag
     4141 cgacagtggg caaaaactgc catattggtg caggagctgt cttagcaggt gtgatcgaac
     4201 cagcatcggc caaaccggtt atcgtagaag acggcgtact ggtaggtgcg aatgcagtga
     4261 ttgttgaagg tgtccatatc ggaaaagatg cagtcgtggc agcaggagca gtggttttag
     4321 aagatgtggc agcagaaact gttgtcggtg gaatacctgc acgtgtatta aaaataagtg
     4381 atgatcagac aaaagaaaat acagcattga ttgctgcgtt acgcgaatta tagaggagat
     4441 ggagaaaaat gcgcgaaata gatacctgta ttgtgggggc aaccggatta gtcggccaaa
     4501 cgatgctcaa agtgttagaa gaatatgatt ttccagtagg acgattgaaa ttattagctt
     4561 caaaacgttc agccggtaaa gtttgtgtat tcaagaaaaa gaaacacact gtacaggaat
     4621 tgacagaaac ttcatttgaa ggatatgatt tagcgctatt ttctgcaggt gcttctgtat
     4681 ccaaagagtt tgcgccaatc gcacgaagtc agggactatt tgtcatagat aattcctcag
     4741 catggcggga agattcggat attccattaa tcgttccaga ggtcaatctg gaagactata
     4801 cattgaatcg gctgattgca aatccaaatt gttcaaccat tcaagcggta cttccgctta
     4861 aagctttaca agaagccttt ggtatccagc gagtcaatta ttcgacctat caagcagttt
     4921 ctggttccgg gcaaaaaggc attgaagatc ttcatcgtac gcaaaatgac gaggaaccag
     4981 ttttgtatcc ttataatatt agccaaacgg tgattccgga aatcgatcag cctttggaaa
     5041 atgggtatac aaaagaagaa cagaaaatga taaatgagac ccgtaagatt ctgcatcagc
     5101 ctgctttgcc agtatcagca acctgcgtac gtgtgccaat cgaaaatgga catggagtct
     5161 ctgtggcagt tcaactgcaa aaagagttta cgacggaaga aattagagag tgtctcagcc
     5221 agtttccagg gattcaatta gaagatgatc tagcagcaca taaatatccg acgagcattc
     5281 ttgctagagg aacagatatg gtttatgttg ggaggatcag aaaagacact tctttagaga
     5341 acggattgtt gttctatacg actgccgata acatacgcaa aggagccgct gcgaatgccg
     5401 tacagattgc tgcagcttta atgcagaaaa tagcggaggt ggcaagatga gtaatacgct
     5461 gaaagtagcg aaattcggcg gaacatcgat gtctcatgaa gagcaattca aaaaagtccg
     5521 tacaattgtt ctttcagatc ccaatcgcca agtgatcgtt gtttctgctt taggcaaacg
     5581 tacgtcagat gatgacaaag taacagactt actttatttg atttatgctt accttcagca
     5641 tcatgttgat tggacacccc tttggaaaag gatttgtgat cgtttcattc aagtaagaga
     5701 tgttcttgcc ctgcagttac ctattgaaga tgaactagca cgcatacaga aacaactgga
     5761 agacgaaaag ataactgaag attatttagt aagtagaggt gaatatctga ctgctcaact
     5821 gatggctgag tatcttggtt accagtttat tgacgcaaaa gacctgattt tctttgacta
     5881 tgatgggaaa atcgatcagg aaaaaacagc acaggaaatc acaaaagcat ttaaaagata
     5941 tccgaaaatg gttgttccag ggttttacgg ggcgaaccct gcaggaaaag aaaaattatt
     6001 gggccgtggg ggaagcgaca ttaccggcgc gatactggcg aagatgttgc aagcggaaag
     6061 ttatgagaat tggacggatg tttcaggaat catgatggct gatccgcgaa tcatcgatca
     6121 tcctaaaatc atcgaggaaa tctcttttag agaactgaga gagatggctt atatgggggc
     6181 taacgttctt catgaagcag ctgttttccc agttcaagag gctgatatcc ccatccaaat
     6241 aaaaaatacc aatgatcctt tcgctcaagg gacaaaaata tcgaatcacg aaatagaaaa
     6301 ggaaaatgga ctgacaggaa tagctggacg caaggatttt ctttccatca ctttattcaa
     6361 acgtcatatg tccgatgaaa tcgggtttat ctggaaagca atgagcattt ttgctaaaca
     6421 tgagatatca atcgaacata ttccttcagg gatcgacaat atcggagtga tcgtctcagc
     6481 agaagcgatt gccgataagt tatttctcat cacgaaagaa ttaaaagaaa cacttggcgt
     6541 ggaagaaatc gtcgtgatcg aagacttagc tttgatctct gtcgttgggg gtccgcataa
     6601 agaattgatt ggattatctg ggaaagtgtt atcgatattg aacaaattag aaattcgcac
     6661 atcgatctta tcacaaggag cacaggagtt gaatctgatc atcggtgtac cgaataatca
     6721 atatgaaaca gtagtgaagg gtatctatga agggatggtg aatacggatg ctgaaagaat
     6781 atcgaatggc actccatcaa attcctgaaa tcgggtttca tgaattcaag acaaaaaaat
     6841 atttatatga acaagtaaaa aacagcgagg ccgttatcca tgaaatcggc gaaacaggac
     6901 tactccttta ttttgataat cagcaagaag aaacgattgc ttttcgaaca gatatcgatg
     6961 cactgccagt caaagaagaa acgaatctct cttttgcttc taagcatgaa ggttatatgc
     7021 atgcatgtgg tcatgacgga catatggcga tgcttttagg gttgactgac tttataacta
     7081 aacacattga ggatcttatt tataatgtag tactgatcta tcagccatca gaagaaattt
     7141 ccggtggcgc acaaagcgtt attcagtcag gattgttgga taaatatgaa gtacaagcaa
     7201 tatttggctt tcatctatgg cctggactag ctaagggaga agttttctct cgaccaggac
     7261 ctttgatggc acaaagcagt gaaacagaca ttatcattaa aggccagtct gcacatattg
     7321 cttctagtga aaaaggaatc gatagtctgg aggcagctgt acgatttttg aagaatgttt
     7381 atgattttga ccggcaatta ccggcagagt ggaatcatct aatgaaattt ggccaaatcc
     7441 aaggtggaac gatccgaaat gttttagctg gagaagttat tgtatccgga agtatccgtt
     7501 cttacagttc agatatgcaa aattatttga agaggggatt agaccattta gcagaacagt
     7561 ttcactctac agctactatt tcttttcgct ataatgacgg ctatccagct gtaatcaacc
     7621 atagaggctt atatgcagca ctgttagatt caaacaaact acatgaatta gatgctccag
     7681 ttcttcaagc cgaagatttt ggcatatata cgcaacaata tccatgtgta tttttctttt
     7741 taggtgtcgg tgatacccct gctttacatg atccgacgtt cgattttgat atggatgttt
     7801 tagaaaatgg tgtagaatgg tacaaaacga ttctttttac agaaaatctt cttgtaaaaa
     7861 tcgatagcag ttcaaaagag gagcgagtaa ataatgatga atttatccaa tcgatttagt
     7921 cctaggcttt ctagaataga agtatcaaaa atacgacaat tcgatcagca aatatcgtcg
     7981 ataccagatg tgatcaaact caccctggga gaaccggatt ttccaactcc tgaacatgtc
     8041 aagcaagcag gaatagcagc gattgaagaa gacttttctc actataccgg aatgagagga
     8101 ttagaggaac tgcgggaagc ggcttgtata tttcagcaac agcgttatgg attaacttat
     8161 gatccgcaaa cggaagtttt gacgacagtt ggtgcaacgg aagcgattgc ctctgcgttg
     8221 ttatctgttt tggaagaagg agacaaggtt cttattccag cgcctgctta ttcaggatat
     8281 cagccattag tggaactagc tggtgctgaa ttgatcccga tagatacttc tgacacaggg
     8341 tttgtctgtc agccggaaca atttgaacga gcatttgaac aatatggatc agctgtcaaa
     8401 gcagtcattt taaactatcc taacaatcct actgggacga cattgtctgc aaaacaattg
     8461 aaagcaatcg ctgaggtttt aaaaaaatat ccggtatttg ttataagtga tgaagtatat
     8521 gctgaattga cttatagtgg aacgcatatg tccatagcta cttatcttcc agaacaaaca
     8581 atcgtgataa gcgggttatc caagtcacat gcgatgactg gttggcgagt gggctttatt
     8641 tttgctcaaa aagcccttat tgacgaacta attaaagtcc atcaatatct ggtgactgcc
     8701 gctacaacga tgtcgcaaaa agctgcggct gaagcgctaa taaatggggt gaaagatagc
     8761 gagaatatga aagagcaata tcaaacacga agagactatc tggtccagca attggcgcct
     8821 ttaggatttg aagtgaccca gccaaacggt gcattttact tgttttgcaa actcccagct
     8881 gccattcaaa ctgactcttg gcagttttgt gtagaattag cggaacaggc aaaagttgcg
     8941 tgtatcccag gaattgcatt tggcccagaa ggagaaggtt atatccggat ttcttatgct
     9001 agcagcatgg aaaaattaca tgaggcctgc aaacggatca aagacttttt aacagaacag
     9061 tgattcatag attttatcaa aaaaacaatc gttaattttg cggtatattc tgcaaggaga
     9121 cgattgtttt ttaaatttag ttcaaatgaa cgcgtttctt ttgattcata actaatgtaa
     9181 tgttaagttt aaattgttta gaatgatttt aaaagaatat tcaaggaggt ttcatcaatg
     9241 gaaaaaatca aagttgtcat tgtaggtgca tctcatggag ggcatcaatc gatcttggaa
     9301 ctattgacac gatacggtga aaatgtagat atcacgctat ttgaagcagg agactatatt
     9361 tccttcatgt catgtggtat ggagctgtat ttagaagaca aggtaacaga tgtaaatgat
     9421 gtacgtaatt tcagaccaga aaactttccg caatccaatg ttcatatttt aaataatcac
     9481 gaagtaaaaa cgatcgatgc cgataaaaaa acagtaacgg ttactagcgc atcagataac
     9541 gaaacaaaag aatatcctta tgacaaattg attttgagtt ctggtgtcaa accaaacaat
     9601 ctgccagtac caggagcaga tctagaaaat gtctacttga tgcgtggata caattgggca
     9661 accaaaatca aagaaaaatt aaatgatccg gctattaaaa aaatcgctgt cgtaggtgcg
     9721 ggttacattg ggatcgaagc agcagaagca agccgaaaag ctgggaaaga agtcgtattg
     9781 cttgatgtga tcgatagacc tttaggcact tatttagatg ctgagatgac agatatttta
     9841 gaacaacacc taaatgaaaa cggtattgaa gttttgacaa atgcaaaaat cgaagctttt
     9901 actggtaacg gaaaagttga ggcaatcaaa acaagtgaaa aagaaatccc agcagatcta
     9961 gtcattcaag ctgccggtgt gaaggcaaat acagaatggt taaaagggat cgtcgactta
    10021 gatgaacgtg gctggatcca aacaaatgaa tacttgcaaa cgaatcttcc agatgtgtat
    10081 gcagtaggag atgctacact tgcttattct atcccagcac gcaaaaaaat gccgatcgct
    10141 ttagcgactg ttgcacgtag agaagcacgt tatgtagtaa aacatctttt tgaagaagta
    10201 ccaaacaaac cgttcggcgg agtcgtaggt tcctctgcat taagtgtctt tgactatcat
    10261 tttgcagcaa gcggattgaa tagctttaca gcagcaaaag caggagtaga tgtacgagtg
    10321 tcttattatg aagatacgtt acgaccaaaa tatgtaccag aagaacaagg aaatccaaaa
    10381 gtgtccatcc aattattctc ggatccaatg acccatcaat tgctaggagg agcagtatta
    10441 tctacctatg atgttacagg tcaaggaaat gtattggcac tagcgatcca aaacaaactg
    10501 actttagaag aactagcaga agctgacttc ttcttccagc caggctttga ccgacaatgg
    10561 agcatgttga atctagccgc tcaacaagcg ttaggagaag aaccatttgt agaataagaa
    10621 aacgaaatat ttgaaaagag gttgtgaaaa agctgccttg catcaagtaa taagaacagc
    10681 caagtaaaaa cagcttctta tgtttttaac ttggctggac ttattaccgc agatgcaagc
    10741 ttttgaacac cgtttatttt caaaaaaggg actgtgacac gatttgtgta ccgaccccca
    10801 aaagttagac caaaaaatct aacttttgga ggtcggtatt ttttatggtt aaatatactt
    10861 ttgaacttaa attaaagatc gttcatgatt atttggatgg caaaggcgat tggaccgtaa
    10921 aaatccagat caagagattg aagcggaaat gcttaaaatt cgggagaagc acaaggatta
    10981 tggctgttta cggatgacca atgaactaag aaaccgcggt ttctctatca ataaaaagaa
    11041 agttcagcgc ttgatcaaga agctaggaat aaaagtaacg gcatacacgc gtaaatcacg
    11101 tcgctacaat tcttatcgag gaaaagttgg agcagtagcc aaaaatcgga tccatcgtcg
    11161 tttctacacc agtatctgcc atcagaaaat cacgacggat acaactgaat ttaaatacca
    11221 tgaagtggat gccaaaggca ttatccgtca aaagaaattg tatctagatc cttttatgga
    11281 tatgttcaat agtgagattt tatcctatcg tatctctgaa aagcccaatg cattagcggt
    11341 aatggaaggt ttggaggaag ctatccagac cactaacgat tgtccctatc gtcgtacgtt
    11401 tcactcagat caaggttggg cgtatcaaat gaaagcttat agaaacaaat tgaaggagta
    11461 caaaatcttt caaagcatgt ctcggaaagg gaactgctta gataattcac ttatggagaa
    11521 tttttttggc ttattaaaac aagagatttt tcacgggaaa gtttataact gtttcgagga
    11581 attaaaatca gccatcgata gctatatcta ttactacaat aatgaacgga taaaacagaa
    11641 attaaactgg caaagtctcg tccaatttcg taaaacaacc gtaacggttg tgtaaaaata
    11701 tcattttaga gcataaaaaa aaggagtgga tttctccact ccaaaaaagt ctaacttttg
    11761 ggggtcacta cagtgtcaca gtcctttttt tagtaaaagg atacatttct tccttgtata
    11821 ttccatgcgt gttcgtgaga aagcatatat aatttaatta acagatagtt agggggagaa
    11881 aaaatgattt tttcaattac aatgaaactg gtgttcgggt tgatcggttt attattagtg
    11941 gtacgtttgc ttgggaagaa atccatgtca gaaatcacac cgtttgattt agtctatacg
    12001 cttgttttag gtgggatctt agaagaatca acctatgatg acaatgtcca tgtagggcat
    12061 gtcctatttg ccattgcgct ttgggcagta atgatttatg gtatcgaacg tatcgtccaa
    12121 aaaaatgaaa aggtcaatcg ttgggtcaaa ggcgagccat ctgttctaat caaagacggc
    12181 gtcatcaaca tgacagagtt gacaaataac catatcgaga tggagcagtt gcgagcaatc
    12241 ttaagacagc aagaatgctt ttttcttgaa aatgccaaac atgttatcct agagaatgcc
    12301 ggtcaaatga gtgtattgaa aaaatcagat gaagacaaag ctttatcgat cctgcttgta
    12361 gatgaagggc aaattcagca taaagtgctt cagtccaatc aattgactga agcatggctt
    12421 atggagaact tgaagaaaga aggctatgca gatgtaaaac agctgattta tgttgaatgg
    12481 tcagaagaaa aagggctcta catactaaat aaagaagacg tcatgcatga atcttaccga
    12541 atagatggat agatagacag aggttgtgaa atcagcgcct tggcttgtta ctcaaaacta
    12601 agcgactttt tcacaacctc tgatatctaa tatacgttgt tccagaagtt gcttcttcga
    12661 aaaataagcc aaaattccac aaaaatttaa gaagcaattt tcgaaaaatc ctttcttatt
    12721 ttttgaagtt gatcacttct gtcacaacct ctcatgtcac aaaccatttt tttgtttcct
    12781 ttaactcaat ataccgtacg tcttcccaca atatcggctg atggtcaatc ataaagtaat
    12841 cttcataggc ttcaccaatg aagtgccctt cgacctcatc caagagtctt ccaaattcat
    12901 cttttaaatt gagctgaatg attactgctt tacgagataa aaaggcttct gtcagtatac
    12961 tatcgatttc ttgcaatgtc atttgttgtt taggaagagg ctgtttgcgt gcttcttctt
    13021 catttttttg ataccgttga ctagttcttc catcgcaaaa gcagtccccc attttaatcc
    13081 aaatggccga tctcgatact cgttatagtc aatgaatttt tttgctgtcc gtctcatctt
    13141 ttccaccttc taatccactc ataccacctg catgcccgcc aacgagagaa ctacgtgcga
    13201 tggcgcgtcc tccttgcaag agactatgcg catggatgat tgattgaaag ccgtattttt
    13261 ttctaatcgt atcgacaaca aaatcaactt tcgcttcatt tatctgcttt tcaggatctt
    13321 caaaaaggtt aagctgtaaa cctgtcgcgt atacgaggtc ggaacagttc acgcctacac
    13381 gccgaatatc ctggtagtgg taatgctggt ggaaaatggt caataaagca tctgctagat
    13441 ctctgctagc atttgtcgct tcgattttca tttgctggtg aaacccggtc ttgccatttg
    13501 gatcaagata gcctaaagag tatcctaccc ataagctgac tacttgtgtt tttgctgcag
    13561 atcgtctcaa gcgagtgccg acttggtcgg ccatttcttt gataacgatc tcgatttctt
    13621 ctcgtcttgt ataatctcta tctaggattt gactatttcc gatacttttt gattttggtg
    13681 tatattcttg tcccaaaaaa cttcgatcga tgccccaagc atgggcataa agctgagtac
    13741 cgatgacccc tagcttgctt ttcaagtgat aataatccgc gtgagctaaa tcataaacag
    13801 aatgtatacc catttgtttc agacgttttg ctgttcgttt gccgattccc caaaagtcag
    13861 tgatatctga gattttccat aacttttctg gtacatcttc ataacgccat tcagcaaaca
    13921 tttgctttcc gtcttttgag ccattgtcta aggctagttt agcaagtagc gggttatcgc
    13981 caattcctat agtcgtgtag attccggtct gctcaaacac atctttatgg atcagtttag
    14041 ccatgtcgta cgcatttgtt ttaccaaaga gtgccatgct atttgttaca tcaaggaagc
    14101 tttcatcgac ggaatagaca tgatggttcg cttcgtctgc atatttttta tagatagaat
    14161 tgatttctct gttcttttcc atataatagg ccatccttgg ggcaacaata tataaatcac
    14221 ttggataggg gaagggaaga tctctggcac ggctgatatt ggtgatgccg taagcttttt
    14281 ttgctgcagg actggaagcc aagattaatc cgctaccccg tgtttctgtc gaatcggacg
    14341 gataggacat gactactaat ttagttgtaa ggggatcaag atccttttca acgcactcta
    14401 cggaggcgta aaaagatttg cagtcaatac ataaaatatc tcttgaaggt tctttttcat
    14461 aaacaaatac cggattttga ttcgcccaca taacaatcac ctcttcaaca caattagaac
    14521 acacgttctc tttccgtgtc aataaaaaac agcaattttc aaatacgagc aaaaggctca
    14581 catttggaaa ttgctgagag gttattttta tctatttgtt tttagctgag atcgttgatt
    14641 tttttatcaa acatgaacag cttctggatc gatctttgtt gtcccatttc caatgtgtgc
    14701 aacataaaat tccggagcat agccgacaac tttcatgtat tcatcataga cattgttttt
    14761 gaaggcaggt atattttctt ctttgacaag agcaatcgca cagccaccaa atccagctcc
    14821 tgtcattcgt gcacctaaga caccttcttg tttttgagca gcatctacta gtgtatctaa
    14881 ttcgatccca gttacttcat aatcatagcg aagagagtgg tgagaatcat ttaacagttg
    14941 accaaatgct tctaagttcc cagcttctaa ttcagctttt gccttcaaag tccgctgatt
    15001 ttcagaaacg gcatggcgag ctctgcgtat caatgtatca tcgccgattt ggtcaatagt
    15061 tgattcaaat tcttcttcat ctaagtctcc taaagaagag ataggcaaag ttttttgcaa
    15121 acgtgccaag gcttcttcgc attcgctacg gcgttcattg tattttgaat ctgccaattc
    15181 ccgccgctta ttcgtattca tgatgacgat tgcataacca tctagtttca ctggaaccat
    15241 ttcgtatttc agcgtatttg tatctaataa gattgcatga tctagctcac cgaagccgat
    15301 ggcaaactga tccatgatac ctgaattgac accgatgaat tcattttcta cttttttacc
    15361 tgtttggact agttccaagc gatctgtttt taaatcaaac agatcttcta atacaacacc
    15421 tactaataat tctaatgaag cgctactgga aagtcctgca ccattaggga ttgttccttc
    15481 cactaataac tcaaacccat gatcgattga gtgtccagct tcttttaatt taaggaccat
    15541 tccttttacg taatttgccc agtttgcttt tttatcatat tctagctcat ctaaagtaaa
    15601 actaatgaca ccgatatcgt caaagtttgt ggaataaact aatattttat tatcgtctcg
    15661 aggtgctgct acaccttgcg ttccataagt gatggaagcg gggaagacat ggcctccatt
    15721 gtaatctgtg tgttcaccaa tcaaattgat gcgtcctggt gagaagtatt gtgtagtttt
    15781 tttaggtccg aatgtttcgg caaattttgt gtttaagctt cttttgattt ccatgatgag
    15841 ttattccttt cttgttttga ttttgaattc tgttactgtt tcaaatactg atccggcttt
    15901 taaatgaatc gaaccaaatg ctgagaagcg ctctgcacct ggagctgttt gagtctcaaa
    15961 tgtgatgcca ccgtgatgtg ccagttttcg gttgcgcatt tctggtgttt ctgtgccgaa
    16021 atttgcagtg aaaataacca cactagaagc atctgtagca atatctactt ggattctttt
    16081 atcggggcta gtcaagcgag cggcttcttt acccaagccg gtttctttta agaaaaatgg
    16141 atgatcgatt ccgtcaacaa gatttttttg atccaaatca ctagcaaaaa cggacgctaa
    16201 ttttttaggt atttgaaaat cgaaagcact tcctgcagca ttttctttta cccctatagg
    16261 aatactatct gttcgcaaag gagcataagc ttcgctattg agccaaagct catgttgatc
    16321 gattgactga gaagcatctc ccgtcaagtt gaagtaaaca tgatttgtcg gattgaataa
    16381 agtatcttgg tcactggtgc cgcgagaggt cactcgccag atattatctt ttgtcaaagt
    16441 atagcggatt tccacatcca ttgtacctgg aaaaccatgt tctccgtcgg gacttgtagt
    16501 agtaaaaatg acagaagctt cattttcgcc attcaaaatg acataagacc atttttttaa
    16561 ttcaaatcca ggagcaccac catgcagatt atgtccagtt tttggatctg tggctaattg
    16621 gtaagttttt ccattgagag aaaagcggcc attttcgatt ctccctgcgg tgcgaccgat
    16681 agaagcaccg atatacggat ctttttcgat atattcttca gcggtgtcaa atcccaaaac
    16741 taattctcga tcatttgatt ttaaactgac gattctagca cctaaatcag agatagaaag
    16801 tgctagcttg tttttgttct ccaaggttat caagtgataa ccttggccaa aatctttttc
    16861 tgtaatcttc attttcaaga tcctttctta atgggaagga actactgcat cgtgaagttc
    16921 atcttccatt gtatcagctg gagccaattt gttttccaat ttctcaacga tttgtgcatg
    16981 ttttttctct gtcaatgtaa tcttccaagc aaaaataata gcggagaaaa tcatcaaagc
    17041 agcaggagca tagaatgcat aagctttgaa agtcgttacg ccatgagcag tgatatcagc
    17101 agcagtagca ttaccagtca tcccagaagc gatagcgaca aaccctacga tcccgttaga
    17161 caaagcacca gcgattttat ctagtaaagg gcgaattgat agagtaacag cttcgttacg
    17221 gataccattt ttccattgac catattcaac agagtctgtg atcgtcatca aagcggataa
    17281 gaaaatcaat tgttgaggga agtagaacaa tactagcccg atgataacga ttggcaaact
    17341 tgaacctgcg atcgtaaaga aaaagtaaga agaaatcatc attgcgatcc ctccaaggta
    17401 aacgtagcga cgagagatga cgcgtgtaag cattgggaat agtggaacag ctactaaacc
    17461 aagtaaggca gcaactagac caacgatcca aaattcagtt ggttggccaa gtacatattt
    17521 gaagaagtag aataatacgg cagttgttgc tacattggca atcgcataag cgatataagc
    17581 aagggcaagc cacatcaact gatcattttg agcaactgct ttgaacacat ctttgatccc
    17641 agctttttca ttttgctgac ggatcgcagt atcgacttct tttgtgccca aggcagtgat
    17701 aatcgcagta ccgcctgaaa taacagcagc aatcacaccg aaccagaacc agcctgaagc
    17761 gccttcaata tgtttatgag taacaagata ggtaaagaaa gtaacgatag ggacaacgac
    17821 tagagtagta ccatttgcac ctagagaaga gccgaaacga gcaaacgtag caaatttttc
    17881 acgttccttt gtttccgtac tcaaagctgg aatcattgac caaaaagcga tatccttaaa
    17941 tgaataaaaa ctatccaata agatgaaaac gattgcaaat aatatgataa ataaagtcgt
    18001 attatgagta gctaagccaa agaaattggt aaagattgct accaagagta cggaactaac
    18061 aacgccgccg ataactaacc atggtttgaa tttaccgtat tttgtacgag tattatcgat
    18121 gatgccacca atcaaaggat caaaaataat ttcaactaag cggataccta caactaagct
    18181 tgtaactgta gcaatcattt ttgcattcgt tgccttatct gttcctgcga acatctggct
    18241 tgtcacgaat accataaaat aagtgcttaa tgtaacatag aatgtgtcgt gtccaaaagc
    18301 gcctaaagcg taggacacac gttggatcat tttgcttttc ataatctgta ttcctctcct
    18361 gttcaatata taatactaag taaaccggtt acaacgaata acaaagatct tttaatgact
    18421 taaacttaac aagaaatcgc ttccttttta atatatttct gtgactttta tttaactttt
    18481 tgttgcatta tactaaagga gtaaggaggg atagacgtgg agcaatcgat ttttttaaaa
    18541 aaggaacatc aacaagtaga aaaagaattg tattttgatt tttgtggttt ctctaaaacg
    18601 atgccgtttc attcttttgg tcctgctatt cgcaatgatt acatcctgca tgtcgtgatg
    18661 gaaggcaaag gatcttatca tgtgaaaaat caacaattcc aattgaaaaa aggtgatctg
    18721 ttcttgattc gtccaggaga ctcgacgttt tacttatcag atggagaaga tccgtggatg
    18781 tattgctggc tttcatttgg tgggagtgct gcagaagaaa tcattcggca gtcgcttttc
    18841 agagaagacc aatacacgat ggtctcttct gacatacaaa aatatatcga cattatttta
    18901 gaatgcatga aagatacgga agaaaccttg gaaaatgaat tacagttaac agcattaacc
    18961 taccgttttt tatcagtcct tttaaaagat ggcggaaagg tcagcggtgg tgtacaaaag
    19021 gttcattcct ctttagctgt tgaaacagtc aaatacatcg aggcacatta tgctgaaaaa
    19081 ttgtctgttg aggaaatcgc ccagcattta gcggtcaatc ggagccattt gtcgcgagta
    19141 ttcaaaaatc atatgggtac cagtatcaag gaatatttga tcggggtacg gatcaatcga
    19201 ggggcgtttc ttctttctct gacggatgaa tccgtggaaa gtattgccta tcaagtaggg
    19261 ttcaatagtc tagtcgtatt tagccgaatg ttcaaaaaga gtaccggaga aacggctacg
    19321 aactatcgta aacgtatgaa aaacgaaaat tttaagaata ttgctttgga taccttgaaa
    19381 aaacagttgg aaaaacaaac cgcagtttct tggacaacct gattagtctg gttaaaatga
    19441 caagcatcgg caaaagaaac ttgtggcggt tatgctacac tgtaaagcga agactaaaaa
    19501 ggaggaaaag ggatgcgtac acttatcatc attagccatc caacgatcga agaatcttta
    19561 aatcagcaat ttttcaaaga agcttctgct ggtttaccag ctacttggca tcatttggaa
    19621 tcaagttatc cagacggaaa aatagatata gagactgaac tacagctctt ggcccagcat
    19681 gatcggatca ttttccagtt tccgttttac tggtatagtt ctcctgctca tttaaaaatt
    19741 tggcaagata atgtcttgga acgagcagca catgtattaa aagggaaaga actaggaatc
    19801 gtattgacaa caggtgtggc ggaaaaagaa taccaagctg gaggaaaaga agaatatacg
    19861 atttccgaat ttttgaggcc gtatcagcga atcgcaaata aattccatat gacctatctt
    19921 ccgccattcg ttttagcaca atttatgtat ttatcccagg aaaaacgttg ggagaagcta
    19981 attgcttatc agcagtatct ctcattggaa ggcaaaccat cgctgactca gcggattgac
    20041 tggtttattc aaagagtaca ggaaaatcag aaaatgcagg aagaagaatc tgaaaaacaa
    20101 gcatatatcg tagaagcact gactgaggca aaagagcaga tcgaagactt gacctttaca
    20161 ctgcaagaaa tgaaaggaac ttcattatga caaatgattg gctgatccaa caattagatg
    20221 aattaagcga aaaatcagaa tatcaagacc gtgcgctctt atatgaaata aagaaactga
    20281 tggctgagat agacgaacga acaatgcagc tgcaaggaga aatcgacgga caatcttgga
    20341 atcatctcaa ctggtgaaat tttcttataa ataacaaaga taaaaatgtg cacagcgaca
    20401 attatttgct acaataagaa tatgaagcgt ttgataacgc tattatacat aaaaattggt
    20461 ggtgaaaaaa atgtatgatg aaaatgagtg gggagaaaat gttcggatga cccgaagaag
    20521 acagcaaaaa caggcagctc gtaagcggat gatcatgatt ggggcagcag ttgccgtgtt
    20581 tttgatcata gcaggaggag ttgcgggtct atattttcat ggaaaaaatt cgtcagaagc
    20641 gaaaagtacg accataacgg aaacaacgac aaagaaaaca gagacgaaac aaacaagtga
    20701 gtcatcaact aaaacaaaag agacacaaac ttctgaagaa aaaacagcaa atggtttgcc
    20761 agagcaactt caagcaatcg taaatgatta ttcgaaaaag atcgaagaaa agacgcctgc
    20821 attgattgaa gaataccaga cagaaattca gggaaatcaa gagggaatag ctggtttatc
    20881 agcagttgct aaccaaaaag cacgtgagct tcaagcaatc tccgacgaag ggatcagaaa
    20941 attacgtgca gcgtatcagg cagcagaaaa taaagacggt gtagacttag atactttgat
    21001 caatcaatta tcggctaatt atacgaatca tgtcgctaag atttccgata tttatttgca
    21061 gactagcgcc tctcttcaag cagagtcaac aagcacacaa gacacaacat cttctgattc
    21121 tgagacagca gagtcggtag aagcttctga aatgcaagct cgaacaacac aagacacaac
    21181 agattcctct gaaactaata ccaacagcgc gacttcacag gctgcgacaa ccgtagtttt
    21241 ggaaggagaa ggtccaaatc aaatagccga acgaactggc gtaccagtag aaacgatctt
    21301 gtcgctgaat gggatgagca tggagaatta tttcctgact cccggtgaga cgctaaaatt
    21361 gaattgataa aagcaataaa aaagaacgaa gcttccacta aggtgaagct tcgttctttt
    21421 tttattttcc ggttcttttt tcgcgtacaa agtaatggat cgtcatgtaa agcaagaaaa
    21481 cgagtccaat aatcaatgag attctcgttt caggattgat aaacatgaaa atcaatacga
    21541 tagctaaaaa agcaagtgtc acataattcg tataaggaga tagtggcatt ttaaaaggat
    21601 gatcatgcat tttatctgcg tgcttctttt tgaagcgaat ctcgctgatc aaaatcacta
    21661 cccaaggaac cataccaggc aaaatacttg agctgtatac gaaaacgaaa atattttctg
    21721 actggttgat gaacattggt agaagataat ttaagatcag accaattaag attccacctg
    21781 agattgctac gacaggataa aaaggcgcac cgtgacgaga tagtttcaga aattttttag
    21841 acatctttcc atttatggca agtgtataga ccatacgact tgcgctgaaa atccctgaat
    21901 tgcaacctga taatgccgct gtaatcacga caaaattaat cagtcctgca gcaaatgtaa
    21961 tgccgacttt agaaaaagtt tgtacaaaag gtgaaccaat ctcactcaat tgattccacg
    22021 gatagatact cacaatgata aaaatagcac caatataaaa aatcaagatt cgaccgatag
    22081 tggactggac agcttcaatg atcgtctttt taggattctc tgcttcacca gcagtcattc
    22141 cgatcagttc gattccttga taggaagcaa caacgatcga taatgcaaag aaaaatcctt
    22201 tgacgcctcc agtgaagaat ccaccatttt tccaaagatt tgaaatccct actgcctgac
    22261 catgattacc aaatccaaat aagattaccc caagacctgc aatgatcatc aaaatgatcg
    22321 ttatgacttt gatgatcgaa aaccaaaatt ctaattcgcc aaacgccttg acagaaataa
    22381 gatttgccag cataagaaac aagacgacaa tgattccggg aataattgcc ggcaaatcag
    22441 gccaccaata attcatataa gatcctacag caatcacttc acttattcct acgacaatat
    22501 attgaaaaat attactccaa gcggtcaagt agccagccac aggatggatg tactcgctcg
    22561 cgtaatttgc aaaagaacca gtcgaaggat caacgtaaag catttcgcct aaagcacgca
    22621 taatcaaata caaaaagaat cctgcaatcg cataggctcc catcactgag ggccctgtcc
    22681 aacgtatggt tgaggaggca cccataaaca gaccgacacc gatcgttcca cctagtgcga
    22741 tcatttggat atgacgggca ctaagacttc gagttaactt ttcttcttcc atacaaaaac
    22801 tcctttcgtt gttgagtcaa caagaaaaaa taatcaaacg tttcaacaca tgtttttcat
    22861 tattaggtta gccttattga actcttgatg tgattctaca gaaaattttc ataaaatgcg
    22921 aggaaaattc gaaaaaaact ttttcatcat tctacttgaa aatgctttcc attttagtta
    22981 aactatattt aaataaaaag ttaggtaaac ctaaaaagga gtgtaagatt tggaacgtac
    23041 ggccattgta attcaagacc tttcagccgc ttatcaaggg aaaactgttt tgaaaactat
    23101 caacttgacg ataaatccac aaaaaattac cggtattatc ggaccaaacg gtgcaggaaa
    23161 atcaacattt atgaaatctt tgctagagtt gataccagct actactggta aagtcacctt
    23221 ttctggacag tcggtaaact cagtccgtaa aaaaatagcc tacgtggaac agcgaagtga
    23281 attggatctt tcctttccaa ttgatgtatt aggcgttgta cttttaggaa catatccatc
    23341 tttacgaatt ggacaaagac ctgggaaaaa agaaaaagaa cgtgcaagac aagctttgaa
    23401 aaaagtaggg atggaagaat atgccaaaag acagatcagc gaactatcgg gtggacagct
    23461 tcagagagtt tttattgcaa gagctctagc ccaaggagca gaatggatct ttttagatga
    23521 accattcgta gggattgatg cgttaagtga acgaaagatc tttgacatct tgcaggaatt
    23581 gaagaattca ggaaaaacga ttttgatcgt ccatcatgat cttcataaag tagaagaata
    23641 tttcgatgag gttattcttt taaataaaca gctgatcgct tccggtccag tacaagaagt
    23701 ctttacatcg aagaaccttc aattggccta tggtgagatc atcagacatt tagtaaaagg
    23761 aggagagaaa aaatgatcca atcctttatt gatggattaa tgaattatca attcctgcaa
    23821 tacgctttgg tcacttccat gcttgtagga cttgcttctg gagtcatcgg ttcttttatt
    23881 attttacgag ggatgtcttt gatgggagac gctatctctc acgcagttct tccaggtgta
    23941 gccatttctt atatgtttgg ttttagttac atctttggag ccactgcttt tgggatgcta
    24001 acagcagcag tgattggttt cgtgactcag cggagccgat tgaaaaatga tacagcgatt
    24061 ggaattgtct ttagctcctt ttttgcatta gggattatct tgatttcata tgcccagagt
    24121 gcgacagatt tgtatcatat tttatttggg aatgtcttag ctgttaggga aagcgatttg
    24181 ttgctgacag cactcgttag tggaatcgtc ttgatatttg tctttttctt ttataaagaa
    24241 ttgaagatca catcattcga tccgacaatg gcaaaagctt atgggctgaa cacatcgctg
    24301 atccattatc ttttgatgtt ctttttgaca ttagtggctg tagtcagttt acagacagta
    24361 ggaacaatct tggtgattgc catgttgatc acaccagccg ccacggctta cttgctaacg
    24421 aaccatttac tgaaaatgat cattacagct gcaggaatcg gtatgctaag tgcagttgtc
    24481 ggtgtgtttt tcagttatag ttacaattgg ccatcaggag ctacgatcgt gttagcatgt
    24541 accgcatttt ttatccttgc ttttttattt tctccaacaa aaggaatttt atttcagaaa
    24601 ggaagcagat agatgaaaac aaaaaaatca ct
//
LOCUS       DAZKKW010000038        24576 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.038, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000038 DAZKKW010000000
VERSION     DAZKKW010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24576
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.038"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(153..551)
                     /locus_tag="ACG8PO_001466"
     CDS             complement(153..551)
                     /locus_tag="ACG8PO_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HFQ0206784.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(548..1321)
                     /locus_tag="ACG8PO_001467"
     CDS             complement(548..1321)
                     /locus_tag="ACG8PO_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HFQ0206785.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(1318..2001)
                     /locus_tag="ACG8PO_001468"
     CDS             complement(1318..2001)
                     /locus_tag="ACG8PO_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HFQ0206786.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(2168..3055)
                     /locus_tag="ACG8PO_001469"
     CDS             complement(2168..3055)
                     /locus_tag="ACG8PO_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0206787.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(3052..3921)
                     /locus_tag="ACG8PO_001470"
     CDS             complement(3052..3921)
                     /locus_tag="ACG8PO_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0206788.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(4058..5344)
                     /locus_tag="ACG8PO_001471"
     CDS             complement(4058..5344)
                     /locus_tag="ACG8PO_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0206789.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            5617..6195
                     /locus_tag="ACG8PO_001472"
     CDS             5617..6195
                     /locus_tag="ACG8PO_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFQ0206790.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            6195..7124
                     /locus_tag="ACG8PO_001473"
     CDS             6195..7124
                     /locus_tag="ACG8PO_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0206791.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            7121..8857
                     /locus_tag="ACG8PO_001474"
     CDS             7121..8857
                     /locus_tag="ACG8PO_001474"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFQ0206792.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(8920..9756)
                     /gene="pheA"
                     /locus_tag="ACG8PO_001475"
     CDS             complement(8920..9756)
                     /gene="pheA"
                     /locus_tag="ACG8PO_001475"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HFQ0206793.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(9753..10262)
                     /locus_tag="ACG8PO_001476"
     CDS             complement(9753..10262)
                     /locus_tag="ACG8PO_001476"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HFQ0206794.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(10255..11550)
                     /gene="aroA"
                     /locus_tag="ACG8PO_001477"
     CDS             complement(10255..11550)
                     /gene="aroA"
                     /locus_tag="ACG8PO_001477"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFQ0206795.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(11563..12654)
                     /locus_tag="ACG8PO_001478"
     CDS             complement(11563..12654)
                     /locus_tag="ACG8PO_001478"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HFQ0206796.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(12671..13837)
                     /gene="aroC"
                     /locus_tag="ACG8PO_001479"
     CDS             complement(12671..13837)
                     /gene="aroC"
                     /locus_tag="ACG8PO_001479"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFQ0206797.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(13839..14903)
                     /gene="aroB"
                     /locus_tag="ACG8PO_001480"
     CDS             complement(13839..14903)
                     /gene="aroB"
                     /locus_tag="ACG8PO_001480"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFQ0206798.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(14936..15958)
                     /gene="aroF"
                     /locus_tag="ACG8PO_001481"
     CDS             complement(14936..15958)
                     /gene="aroF"
                     /locus_tag="ACG8PO_001481"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HFQ0206799.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            complement(16617..17591)
                     /locus_tag="ACG8PO_001482"
     CDS             complement(16617..17591)
                     /locus_tag="ACG8PO_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HFQ0206800.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(17572..19455)
                     /locus_tag="ACG8PO_001483"
     CDS             complement(17572..19455)
                     /locus_tag="ACG8PO_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HFQ0206801.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(19473..21698)
                     /locus_tag="ACG8PO_001484"
     CDS             complement(19473..21698)
                     /locus_tag="ACG8PO_001484"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFQ0206802.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            21813..22847
                     /locus_tag="ACG8PO_001485"
     CDS             21813..22847
                     /locus_tag="ACG8PO_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0206803.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            complement(22914..23927)
                     /locus_tag="ACG8PO_001486"
     CDS             complement(22914..23927)
                     /locus_tag="ACG8PO_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFQ0206804.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(24005..24193)
                     /locus_tag="ACG8PO_001487"
     CDS             complement(24005..24193)
                     /locus_tag="ACG8PO_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HFQ0206805.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
BASE COUNT         7062 a         5341 c         4397 g         7776 t
ORIGIN      
        1 agcatatttt gaaaagaaag tctgatgctt agagtagaat ttcataaaaa tttactattt
       61 cataagtatt cacgagaaaa atacaaacaa aaaagcggaa ataacgctca tccgccttct
      121 tgtttgtgtt tatttcctct tcaaacgctt gtctatgatt cagacttttc tctatcgtta
      181 ttttcatcta aaagttcttg cattccgata atttctcgat attgatgaat cttagtgata
      241 ccaatgactt tgtctacatc aatactaaaa caaataccat tacctggaat atcgatgttg
      301 atgccttgac caatcgcatc cattacttta ttcgtcaaac tatcgggtac taatgtcaac
      361 acaatatctt tttctggcga aatgtccaaa cctaaaaatt tccctgaatc atgtacacca
      421 gaacctcttc catgtaagat cgttccgcct gctgcacctg cttcttttga aaagtcgata
      481 acatcttcgc ccattcctcc gtcaacgatc gtcacaatca tgtgaagatt caatgctaca
      541 agatctttca tttgctttcc cccaattttt gcaatctttc tttttcgatt ctgtcttttt
      601 catcttttcg tttataaata tatcctagta ctaaagtgga taatattggt gtcaatgcaa
      661 tcattgcaac cacaccaaac ccgtcaatga tcggatctct tccttcaatt tgtgcagcga
      721 ttgccacgct cattgataaa ataaatgtcg aacacatcgg gcccgttgct actccaccgt
      781 tatcaaaagc cattgctaaa aagatcttgt ccacttttcg tcctaaaata aatgctagcc
      841 cgtatcctgg gatcaagaaa taataaagag agaatccgac gagcaaacgc cacatagaca
      901 atcctactgc tgcgccgatc ccaattgaaa ttaccatcag catgattttc gatcggattc
      961 tcccctcgct caattcttca acctgtttga tcattacatg aatcgccggc tcagcaaacc
     1021 ctactaagaa ccccattaaa aagccaaggg gaaccagtat ataattgtaa ggcaatcccg
     1081 caatagcact gcctaaatgc tgtcctaccg gtacataagc catattgact ccgtgcagga
     1141 aaaggatcaa cccaatagtc gttatcaaga aacctttcat aatgtcaaag aaacgctttt
     1201 tgcgcatacg aaaactaatg atattcatta aaacaaatag gattacgata ggtgccactg
     1261 ccaaaatgac ttccatggta acgatttcga ttccatctat cacttgctgc cagaaactca
     1321 ttggaagatc acccccaaaa tcatcacggc aagaatcggc cctaacgaag caataccgac
     1381 cattccaaaa ctgtcatttt catttttctt ttgtttgatc atactagtca tccctccggc
     1441 aagggccaag ataaatggaa cagttactgg accagtagtc actccccccg aatcaaaagc
     1501 aatcggcata aattcggaac tggtgaaaaa tgaggcgatc aaaacgccta tgtagcctat
     1561 agccatcaat tgataatagg atagcttgaa tacgacccga agcagcgcaa aggctagaaa
     1621 tactccagtg ccgacgctga cgatcccaat cagcaacact ctgccaatct ctccgtcaga
     1681 tatatcatat acttgttgtc ccaaaacttg cacggaaggt tcagcaatcg tgatcacgat
     1741 cccgatagca aatcccaaac tgaccaaaac ggtcaaactt ttctttttga tcatatattt
     1801 ccctactaga tctccgactt ccatcataga ataatctgct ccaaacaaaa agagcgtcat
     1861 tccgatcatc atgattgccg caccaaccag aaatgaagcg aggtcttctc cttctaacgg
     1921 tgcaaaaaca aacgttaaaa tgacaatcag gacagtcatc ggcaatatag cagcaatcac
     1981 ttctttaaaa ttttttagca tgtatcctcc tcctttactc tttaagttta ccttaaaatg
     2041 aaagagtttt cgagcacaaa cttaaaacgt tatcatttaa tttttctcaa cataaaataa
     2101 cacacgtccc aaaagtatct ttttacttgg aaaaatgact ttgaaggaac gcgcttattg
     2161 tgttttttta ttttacagag ccgatcattc cttgaacgaa ttgcttttgc aaaacaaaaa
     2221 atacgatggc agttggtaaa gtagcgatga ctaatccagc catgatcatt ccatagtctg
     2281 gtgtatagga agcacccata gcagataaaa caagcggaac cgtccgttta tcaggagatt
     2341 gcaaagctac taagggccat aaataattgt tccagctact catgaatgtg atgattgcag
     2401 ccgcggcata ggtattttta gccgtcggca tatacactct aaaaaaaata cttagttctt
     2461 ttagaccgtc taatcgagcg gcttctacta gatcttttgg aaaagattta acattttgtc
     2521 gaaagaagaa gatcaaaaaa gcagtgctaa cagatggcag gatgacaacc aagaaactgt
     2581 tgattcccaa aaccgttccg ttcaattgtg aaaacattcg atataaaggg atcatcaatg
     2641 ccgcaaaagg aaccatcatc gatagcagta gaatattgaa aattaggtct tttcgtttac
     2701 tttgataaat ctcaaatcca tatcctgcca ttgacgaaat cagcaatgca aacacagtgg
     2761 tcaccaaagc gatcatcgct gaattccaaa gggaacgagt aaaattcagc tcattggaaa
     2821 acaaattttg aaaattgatg atcaagttac tgccaatctt tatttttccc gatgtgatat
     2881 ccacgggtgt attcgtcatt cccaaaacca tccagacaaa agggaaaatg gaaataaatg
     2941 ataggatgct taatgctgta tactttaata ctgacccaat tgtcgaacga agttctaatg
     3001 cttgtttttt gacgttgggg cgttcgattg tttctttttc taccatattt ttcatcgact
     3061 ttctcctcct actttcgttt ggattatcga caatatcaca ataaaggaaa caataacaaa
     3121 ggaaactgct gccgcatatc caaaattagg cgtgaattta taactcaaat tatagatgta
     3181 ttgagatagg gtggtcgttg catttgctgg tcccccgcca gtaatatttt gtacttcatc
     3241 aaaaagctgc aatgtcccga tcgtcgaagt aatggaagta aacaaaatga ttggttttaa
     3301 atttgggatc gtcactttga taaactgctg ccatttagaa gcaccgtcaa tagatgctgc
     3361 ttcataaatc tccgaatcaa tattttgcat gcctgaaagg aaaaaaatca tattgtatcc
     3421 agtccaacgc caagtgatcg aaagaatgat cagaactctc gcccaaattg gatgagtcaa
     3481 ccagccaatc ggtgtgtcaa tcataccaat gcccaacaac aagttgttta caaggccaga
     3541 ttgagaaaat agacttttca tgatcagaga ataactgacc atcgaagtga tacatggaag
     3601 aaagatagcg gttcgaaaca accctttata tttcaacttt ttatcattta aaaggttgga
     3661 aataaaaagt gctagaaaca acatgatcgg tacttgtaca agtaaataca ggaatgtatt
     3721 aaaaaaagct tttttaaaag ttgcatctgt caacatccgc tgataattac ctaacccgtt
     3781 aaatgtcata ttgacagggg gaccactttg aaaagacgtg atcaaagcca aaaccatcgg
     3841 aataaaaacc atgattgata tcaaaatgac tggcaagatt aaaaaagcat gcccgttttt
     3901 gaataaatct ttctgcttca ttcttttccc tccaattcta tcattgatta tctacatgaa
     3961 aagacaagaa agacaatatc tgtaaggcag acgtgatctt ttttgtcttt tcgtttgtct
     4021 gatgaaaaag cgaagaaatt cactttttta ccaccgatca attagccaat tgtgcttcta
     4081 cttgtttttg tgtatctgct agtaccttgt ctgtctcttc tccattgatg attcgatgaa
     4141 gagcttcagc tacgactgct tcaattgcat aggtctcatg tccgtaattc acttctggta
     4201 tttcagatgt ccatgttgaa aaatcgtcaa agatcttttg accactgtaa aattcagaag
     4261 cttcttgata agcttcttgt tcattcgcgc taagcatcgt agatactagt ccgatttctt
     4321 tcgcaagagt gcccatcagt tcttcattcg aagcaaacgt tgcttctagg aaatctttcg
     4381 ctgaatcttc tccagctact cctttgatca cataccagcc agcgccgcca aggttagaag
     4441 cttgcgcttt ttgcagattt tctggaagtg caggtattgg tgcaatcttc cattttccag
     4501 attggttttc tgctccttgg atcgttgaag aataccaagc gccagttggg ctagaagcta
     4561 ctgctcctga ttgtatcgca gttactccag cattccagtc agaggtttgt tcgactagat
     4621 cttctttcaa aagagtggca aacaattcaa gaccatactt taaagatgca ttgtcttgga
     4681 tcgtgactgt ctccccatct tcagatgtat accattctcc tgcctgttgc ataatcatcc
     4741 ttactcgacc aaggtcagaa ggattgactt cagcgatttt ctttcccgtc ttttctttga
     4801 catctcgcgc tacttgaata tactcgtccc acttcagatt ctccatatcc tcttctgtat
     4861 aacctgcttc tgccatcaga tccgtacgat agaagtttgc tgttacgccc gaatcgaaag
     4921 gaacaccgta tattttatct cctactttgt ttaccgcaaa tttatacggt gcaaaatgat
     4981 cttcctttac aatagcagac aaattagcga atgcatctgg ataagaagtc aaatagcctt
     5041 gtatcctata atcttcaatc aagacgatat tgggcaatcc ttctgtattc ccgctcgcta
     5101 aagcggtgtt cagtttctga acgatatcat cctgagacat cgtaacaact tctatctctg
     5161 tttcttcgtt ctcataaact ttttttgctt cattcactgc tttgatattg aatgtctcat
     5221 cccacgccca gacagtgact ttttcttctt tgtctgctgt actactagca cttccgcatc
     5281 ctgttaagaa acctgatgct gccgctaaac aaacgactcc agcaattaac tttctgatgt
     5341 tcatttttat ccgctccttt tccatccatt agttgtttac gtttgctaca ttcatcatac
     5401 caaaatttga aaacggtttc tttattattc agatacttca aaaatttctc ctaaaataat
     5461 aaaaaaatac aaatcaacta tgacaaaagg aataatctta gcatctagac caaagatcga
     5521 aatggtgagg tttctttaaa ataaaaatat ccttgcattt tctcctagaa ataagtattt
     5581 ttaaattaca agactattct taaggcgggt aaaaaaatgc caatacgcgt aattcttatc
     5641 gatgatgaac agacgatttt agatggaatg aaacagttga tcaattggtc tgagcacggg
     5701 tttcaattaa tagggacttt ttccagtccg ctgaaagcgc ttgatttttc tttggtacat
     5761 gcaatcgata tcgtggtcac agatgtaatg atgcctgaat taaatggaat cgaattatcg
     5821 cggctattaa aacagcaaca acctgaaaca cagatcctta tcctctctag ctatgatgaa
     5881 tttgatatgg ttaaagattc ttttaaagaa ggcgtcgctg attatctttt aaagcctcga
     5941 ttgagtcctg atttttttct aaattcctta actgccgttt cacaaaaaat caaaaaaaag
     6001 cccaacaaaa gcccaatcac caatcgttat gagcagatct gcgagaatct ttcacagcag
     6061 attgctggac aaactcgtcc attgcttgac cctcttcttt ttgaacacac cttgtttttc
     6121 tgttatatac cgaaaaacgg ccattcagtt caaaacaagg ctctaaacaa aaacagtcga
     6181 tcacccattc attgatggat agcgacgatt cgtttactat ttatcctgtt tctacctttt
     6241 cggaagaatc cggctatgta atcaatactg attccaaaga tacacttagg aagtttgttc
     6301 aaaagtttcg tcaagaagtt tttccaggtg atttttttgt tgtttctgat tcttttgcaa
     6361 tccaagatat caatgaaata ttccaagaga tcagaaattg caccaaagga caacgtttct
     6421 atcataaggg aaagaacctt gcttatcagc atgaacttct tccgttatta accaaccacg
     6481 agcagcaagc aagtagttat ttaacaaaaa tcgttcataa agactacgct ttagctgtca
     6541 aagaacttga gaatttcttc acgacgatat tggaacagcc tattgatcca gcttatttga
     6601 aacatgaggc aatcaatcgt ttctatgcat tactcaacgc tttggctgac gagcatgttg
     6661 ggaatgaaga actttctttg ctcaagatcg cccttccttc tttattagcc gatgctgagt
     6721 atttcgaaga tttttctatt cttgtcaaag aaacacttga gaaactcacc cacttttctg
     6781 caaatgcaaa aaaagacaat ttcgatatct tgactttgat ctacgagttc gtccaggaaa
     6841 attatgagaa agaaatcaat ttacaacttt tatccgaaaa atatcatttt tcatatagct
     6901 atctctcagc catctttact gaaaaatttg gtatcaactt ttcaaaatat ctaaaaaaag
     6961 tccgtatcag taaagccaag ctctttttga cggaaacacg gcttactttg acggaaatct
     7021 gtgagaaagt cggctatacc gaactgggtt atttctccag agtttttaaa gaagagacag
     7081 gaatgacacc ttctcaatac cgtaaaggaa cttttgtaaa atgagaaaaa aaatcaaaga
     7141 gcatgaactc tttgccaaac ttctgttcat tttgttcatc ggtctttttc tgcaagccat
     7201 cgtcatttct ttgtttattt atcatcgatc aagggatgcc tatatccagc tgttcaatca
     7261 atcgaatgat gtggtactaa aaaagattca atctgaattt gaaagtttga atgatacaat
     7321 cgagaataca ttagccgctt ttgattctaa tccagctgtc aaaagctatt tttccaatga
     7381 tccagcccaa catatggaac agcttcagca actaagaaca atccaaaaaa tgaatgactc
     7441 tctttcaaaa atccatccaa tgatcgatta cgatgtattg atctttggag aaaatggaag
     7501 aacttttgtc ggaaacgaca tgctcactgc cgtatctgcc gattcttttt ttcaatctgc
     7561 gattgctcag agagtgaatg aacgtgctgc tgatacacaa atgctttttg cacaccacgg
     7621 attgactctt cgcgataaga aagcacccag cgtcttcttt gtcaaaaaac tgaaaaatac
     7681 attgaatcac gtttacggat atgctgtttt atcgatctca tccaagcaac tagcgaatct
     7741 ttttcaatct gtagtaaatc ctgagatatc gaagatctcc ttcatgaacg aagaacagac
     7801 gatcatcgca tcaaatgaag aaaatacaat tgggagccga tcaatgattt tcaaaaaact
     7861 aacagtaggt gaaacaacga tttcaaatgg acagcagttg acccgtcttc ctctgtaccg
     7921 ccaggattgt acattactca gtcaagtcga tcttcattct ttatctaacc aaatgggcgt
     7981 gatttttccg attattttgt ataatatcgt gacacttgct tttgctggct cattggtatt
     8041 tatgtatttg gatcgacaca caaaatcgat ctaccgcttg attgattcgc taaaacggct
     8101 agaggaagat ggcttaaaag ctactgttcc tgtacaagga agccatgaag tccaattgct
     8161 agggaatacg atcaatcaat tgctaagtaa tgctcagcat cattatgaac agacgatcca
     8221 agacgaaaag aagaaacgtt cgcttgaaat ccaaactatg caagcacaga tacagcccca
     8281 ttttatttat aatacgctga ctagcttgaa atttttgatt tggcagaaaa aaacagatga
     8341 agcagttcaa ggaatcgaca gtttcattac tttactaaga agtacgattg ggaaaaaaga
     8401 agaagtgatt ccagtcaagg aagaaatcaa aagcgtacaa agttacatcg atcttttgag
     8461 tatgcgatat ggcgaaagaa tctcggctaa aataatgatt cctgatgaac tgcagtcatt
     8521 atctatgccg aatatgatcc ttcaaccaat tatcgaaaac gcctatcttc atgcttttca
     8581 caataaaacg caaggattca tcacggtgta cggcaaaaca aaaaatcagc ttcttttatt
     8641 cgaagtgatt gataacggcg atggcttcga tactgaaaat accaaacaaa aaaatgatca
     8701 tttttcagga atcggcatca gccatgtaga tgagcggata cgcctaatgt atggcgatgc
     8761 atatggctta acgatccaat cagtgattgg gacaggaacg actgtaacga tttgtctacc
     8821 gcttcttcct aaaaatcaat cagaatatca aaaataaaga atagccaagc gctgattttt
     8881 ttattttaag ttaaataaat caatcagtat tcttgacttt catctacgcg tctcttctac
     8941 tgtcagcact ggataacagc ccaatatttg actttctcct ccgagcatct tgatttcttg
     9001 gagcgcattc tctactaatg tcattggacg gtccaacaac aaatcgatca cgaaaaaata
     9061 ttctcccaag ctagttttca atggtcggga ttcaatctta cttaagttga tttcccgcca
     9121 gccaaaagct gccagcattt tatgaagcat ccctggccga ttagcaggta gtgtcaagat
     9181 gacactcatt tttactggag ctgactgctg gctggtcatc tttcggtcac cgacgatcca
     9241 aaaacgtgtc tggttcaact cattatcttg gatattctca gctaagatct ccaatccaac
     9301 atgctgtgct gtttctaatg aagcaatcgc tgctgcgtct tctttcggat gttctgctac
     9361 atacattgct gcagcagttg ttgattctgt tgctactaaa ggaacattag gataatgcgt
     9421 ttctaaaaac tgttgtgatt gtgctagtgc ttgtggatgt gaaaggattt ttgtaatctt
     9481 tgttgctgga ttgcctaaaa gctgctgctt gataggaaga acgatttcac ttttaacttc
     9541 gacttctgga tgtttggaaa gaagatcgat tgtgtgatga acagctcctt ccaatgaatt
     9601 ctctacagga acgacagcca gatcgacttg tttgcgaaac aaagcgcgca aacaaacagg
     9661 gatggaagca tacgcagtca attgttctgt aggaaatagc tgacaagccg cctgataagt
     9721 gaatgaactt tctggcccta gatagctgac gttcatatgc ttttgatcct ttctataatg
     9781 gtatcgatga tcttttctgg tgtttgattg gtcgtgttga ttcgatgtgt cgcactttct
     9841 tcataccaag aaagtcgttg tgccaataat gcttttattt cttctcttgt ttttccaaga
     9901 gctaaaggtc gaatagacgt ttcatcttgt tggatacgat caactagaca gtccgcttct
     9961 gcctctaagt aaagaacaat cggctgtgtt tttaaaaaac gccgattttc tgcactttgg
    10021 acaatccctc caccagtcgc gataattttg tcttcatttg acagctttct taaaatatct
    10081 gactccactt ttcgaaagct tttttcaccg taatagtgaa aatattcggc aatcgacaat
    10141 tggatatgtt cctcaatctt tgtatctaaa tctacatact ctttttgtaa acattgagct
    10201 aaccctttac cgattgttgt ttttccagcg cccatgaaac caatcagcaa aatgctagcc
    10261 atgagcttct cctcctgaca caagttctgc taaatcgtca aaaaatgccg gataagagat
    10321 tgaaacagct tcagctcctt ctagttcgca aggtccatca gctatcaaag cagctacttg
    10381 cagcatcata ccgattcgat gatcgcctct gctggacact tttgcgccat gcagaggtgt
    10441 cggcccatgg atgatcagac cgtctgctgt cgcttttatc tttgctccca ttttctgtaa
    10501 ttcttctgcc accgcatcaa ttctgttggt ttcttttact tttaattctt ctgcgtcttt
    10561 gatgatcgtg attccttctg cttgagtagc cactaaagct agaatgggca attcatcaat
    10621 cagacgcgga atgatttctc caccaatcgt tgctttcttc aaatgactcg tcttgacact
    10681 taaatcagcc gattggttct gttcattgtg attcgtctgg gtaatcgctg cgcccatctc
    10741 ctccaacaca tctaagatac cagttcttgt tggattgacc ccgacatttt ttaacaacag
    10801 ttggctttcc ggaacaagaa gtcctgctgc tagaaagaaa gcagctgatg aaatatcacc
    10861 tggaacagtt atctgttgac cagttaattt ttgcggtccg gtcactatga tcgttttatc
    10921 ttccactgtg atccttccgc caaattgacg aatcatttct tccgtatggt tacgggaacg
    10981 ttctttttca acgattacgg atgtgccttc tgcttgtaat gccgcaaaca gcaaagcaga
    11041 tttgacttgc gcactggcta caggcatatt gtatcggata ggtgataaag agcgggttcc
    11101 tgtcacagtg attggtagat actggtcatt ttcttctcct tgcaagtcag cgcccatctc
    11161 gcgtaaaggt tccatcactc gccccattgg acgtttagac aacgaagcat ccccgaacag
    11221 agtagtcgta aaaggctgtc cagccaaaat acccatcaat aaacgtatgg tcgtaccgga
    11281 gtttcccatg tcaattgatt cggttggttg gacaaatgag tggctaccgc gaccatgaat
    11341 cttgatcacc tcttcctctt cttcgatctc gacacctaat tgttggaagg catgtaaagt
    11401 actcagacaa tcttctgcgc gcaaaaaatt ttgtatagtc gtcgttcctt cagcaattgc
    11461 tccaaacatg atgctgcgat gggaaattga tttatctgct ggtatcctaa cagtccctct
    11521 taatccatgt atttgttgta ataattgcat ttatccagcc tcctatttga tccagcaatg
    11581 ataactggta ttagacataa tgattctttt tgcttgtaac aaatcctgtt ggtttttaaa
    11641 actgatttgc agtattccta aaatatcttc cctggtctct tggatcttca aattaatcaa
    11701 agaaactccc gcttttccga tcagtccagt tacttctgca ataacgccag gttcatctgg
    11761 cacatcgata aataaatcaa aaaaggcagg aatcgcgcct tgcttatgga caggcaactg
    11821 gtctcgactt tcttttgccc gattgaaaaa agaaaagatt tccgattggt tctcagtcat
    11881 gatccattct gctatttgtg tcatttcttg ttgccaatct gccaatcgtt ccaataatat
    11941 ctgcttattg ctcaacaaaa tatctgtcca catgacagga tctgaggaag cgatcctagt
    12001 gatatctcga aagcctcctg ctgccagttg tttggctcgc ggatactctt gattgaattg
    12061 ttctgcctga ttgactaagc cagctgccag aatatgtgga aaatgactta gcattcctgt
    12121 tatacggtca tgttctcttg ctgtaagagt tacatatttt gctttggttc cgcaaaatag
    12181 ttcttgcagt ttttcaacat cttttttcgt cttttccaaa ggtgtaaaga tgtaataggc
    12241 attttcaaat agtttagcat ctgctgcctg tatacctgat ttatgagaac cagccattgg
    12301 atgtccaccg acaaaccgaa gatttttctg ctcagcggat gtcacgactt gctgcttcgt
    12361 actgctgaca tccgtaatga tgacgtgatc agacaagttg attctttcaa ggacatcaag
    12421 ataagctaag gttgtacgga cgggaacggc taataagata acgtctgctt gttctgcacc
    12481 tgtttcaaaa tcacttgcaa cgtaatcaac gatctgctgt tctactgctt gctcacgggt
    12541 ttttgactga ctatcccaac caactactat cagttctgga ttggtctgac gaatacaaag
    12601 tgccatagaa gcaccaatca gtccaagccc cacgataaaa acatgttgtg ccatcttatt
    12661 tgcccccttt ttagtaatgt ttcaagtagt cacggtactc cttcactgca gctttcaact
    12721 caacaagcga atcacttggg aattttgaca atagctctgt cgctaccaca gtggctacaa
    12781 cattttccgc aacgacgcta gctgcaggaa ctgctgtact atccgaacgt tctacacttg
    12841 ctttgtaagg ttcttttgta tcgatatcaa cactttgaag tggcttgtac aatgtaggaa
    12901 tgggtttcat gaccccgcga atgacaagtg gttcgccatt ggtcattccg ccttcgaagc
    12961 cgcctagatt attggtacgt cggaaatagc ctctttcttc ttcccatagg atctcatcca
    13021 tcacttctga gcctggtaat gaaccagaag tgaatccgac cccaaattct acgcctttaa
    13081 aggcattgat actaaccaca gcttgtgcga ttttggcatc tagcttcgtg tcccattgta
    13141 cgtagcttcc taatccagct ggaacgcctc caacgagcac ttctacaatt cctccgatcg
    13201 tatctccatt tttctttgtc tgatcgatca aatcttttat tctttgctca actgctggat
    13261 caacaactcg tacttctgac gcttcagaaa tttctttgat ttcttgaatc gagatatttc
    13321 caggtatttc tgcttggatt ccaccaagac aaatcacgtg tcctgctacg atgatgtcaa
    13381 gttcttccag aattttcttg gcaattgcgc cgattgctac acgcatggtc gtctctcttg
    13441 cagaggaccg ttcaagcaca ttccgaagat ctcgatgatg gtatttcata ccgcctacca
    13501 aatccgcgtg tcctggacgc ggacgtgcaa ctcgccgcag tgattggtct ttttcaggca
    13561 cttctgctac agacatgaca gttgtccaat ttttccaatc tttattttct accactaaag
    13621 tgacaggtga accaattgtt ttgccgtgac ggatgccaga cgtgatccga acacgatctt
    13681 tttcgatctt catccttcct cctctgccgt aaccagcttg tctgcgagcg agttcgtgat
    13741 tgatttcttc ttctgacaga ggtaatcctg ctggtagtcc ttcaataatg gctgttaatt
    13801 ctggtccatg ggattctccc gcagtcatat aacgcatctt acagtcctcc taagtaatct
    13861 ttgaatgtct ctgtgggaac aggaacgatt tttgcttgcc caatcttttc tagcaagatg
    13921 atattcaatt ggcttcctct tgctttctta tcatgggtta tcgcgtggaa taattcatct
    13981 ggatgactta gatcgtacgt aatcggcaga tggaattttt gaagtatcct ttctaaagca
    14041 gctgtcgatc cagttggaga caagccgatt ttttctgcat gagaagtgat ggctaccatt
    14101 ccaatagcta ctccttctcc atgactgact accccatatc ctgctgtatt ttcaattgca
    14161 tggccaattg tatgaccaaa atttaaaata agtcgatttc cttggtcaaa gacatcttct
    14221 tccactactt ttcgtttgat ttccaacgta gcagtaatga ttggaacagc atgttccaga
    14281 agatcttttt cgtctttcag ctcgttcagc tgtttccaaa gcgttgcatc tgctatagct
    14341 gctgatttga tgatttcagc gattccttct cgtaaacgac gtaccggcaa agtttttaga
    14401 acctcaggat caatcaatac accagctggc tggctaaatg ttccgacaag gtttttcgct
    14461 ttgtttgtat tgactgctgt cttcccgcca atactagaat ctacttgtgc aagtagcgta
    14521 gtcggaattt gaacgaaatg gatcccacgc atgaacgtcg aggccacgaa tgctgctaga
    14581 tcacccacca ttcctccacc aagtgcaagt atcccatcgc tgcgggtaaa atcattatct
    14641 gctaaaaaat cgtataactc agctgcttgt tctaaagatt tactggtctc acccgccggt
    14701 acgattgaaa ggactggttc aaaattagct tgtctcaatt ggaacatgat tttttctgca
    14761 tatagtggag caacgtttgt atcagtgatc acagctactt tttgaggtct ccaaatcgtt
    14821 tgaagccatg atccagcctg ttctagtgcg cctttttcaa tatgaatatc gtatttttgg
    14881 ttatcaacat gtacagtaag cattcgtttc acttctttct tttaaaaagc tggaattaat
    14941 cgagtgtctg aatcttttcc attgcttctt tcatgatcgc aacttctttc atcatgcgtg
    15001 agtaagtctt ttcattcaac gattggggac cgtctgacca agcatttgca ggatcaggat
    15061 ggatctctac gatcatgcca tctgctccgc ttgccacacc cgcacgagcc attggtggta
    15121 caagttccca aattcctgta ccatgacttg gatcaacaat cacagggaaa tgagttaatt
    15181 ttttcatcaa tgggacagca cttaaatcaa acgtattacg tgtcgctgtt tcgtaagtcc
    15241 gaatcccacg ttcgataaaa atgacaggag atttgtcttc gactgcaatg tattctgcag
    15301 cattaagcca ttcattgatc gtaccggaaa ttccacgttt caatccaaca ggtttacctg
    15361 ttttaccgac agcagatagc agtttaaaat tttgcatatt acgtgcgcca atctgtagga
    15421 tatcgctgta ctcagccacc atatcgatat gcccttcatc catcacttca gtaatcactt
    15481 tcattccaaa ttcatcagcc gcttgacgga tgtatttcaa tccttcttct tctaaacctt
    15541 ggaaagcata tggagaagta cgtggtttga aggcaccacc tcgcagaatt tttgcgcccc
    15601 cggctttggc cattcgcgca cattctctga tttggtctaa tccttcgata gaacaaggac
    15661 cagccatcgt cacgaaactg ccgtcaccaa tttttactcc gtctacatcc acgactgtat
    15721 tgccaggatg gaattctcga gaagtcaatt tgtatgtgtt taaaatacgc acagcatttt
    15781 ctactccttc atagcgtgta aatgctcctt cttgtaaact tcgcgtatca ccttttagtc
    15841 caatcactac tcgttctgtt ccttgatcga ttgagacttt gaatccttct ttttcgatat
    15901 gatcgacgac tttttttatt tgttcttccg ttgcttcttt tttcataatt actatcattg
    15961 tttttcccgc tttcttttgt ttacttatct atcttggctg attttctcta ctttttcaag
    16021 gtggttttac attaattcct actaaatgac cgtgtcctgt tgttctcatt ttcgctcctt
    16081 ctttcctact tatgacccag ataatcaaca aaaaaagatg tccctgtagg aaattgcctc
    16141 tacagtgaac atcttcaacg agccttaaaa agcgctgcaa aaatataaaa tagccactat
    16201 agatgaattt ctctacgtgg ctgtaagtcg tgtttaaaaa tctttagcca tcatagatac
    16261 aaacctggtt tgtttgtacc cacgaagtca tgaatacaaa cctatctaaa atagctaaag
    16321 taatacgtat tcagttttgt aagttttcta gatttcataa aaaatcgctc caaactgatg
    16381 taagaattgt acctagtata gacataaaaa aatcgactgt caatcatttt tatgaaactt
    16441 tttttaattt tctgatagtt aactttgttt ttcagaaaaa taacaattgg ttttattgaa
    16501 gaaaatagtc atgaacaaaa gtatctgaga acttttttac tctcttgttc atgacatata
    16561 gaataaagca gctttgtctt gtaaactccg tttttcaaat aataatctaa agattgctac
    16621 tgaataataa agccgatttc atggtcctct tcgctgctga ttcggtaagg tgactcaaca
    16681 tccgctcccc agctattgat acccccgact cctcttactt ttgcaaaaat cgtaagaacc
    16741 gttcgtcttt ctggcggtag ttcttctata tgtgttgcct gttccagttc ttctgctttg
    16801 aatggtaaag cggaaaaagc aaatggcgta tcctgctgtt caaatcgcaa cgaaaaatct
    16861 tcttgatctt tatccgtatt atccaacgtt tgatttcttg ttagttctac ccatttcgtt
    16921 tccatatgca tgccgcattc ttggggaaca aggtatgggg aaacggggag tcctgccact
    16981 ttataaatac cttcgacacc accagccatc cgatccggat atgtttcccc tgataggcct
    17041 tcatactcaa aaaattttgc tttcgttggc ataatgaacc ttaaacccaa tgcgggtaat
    17101 tccggtaatc ctttttgtcc ataataatgc atattgacaa gcattttacc ctctccagcg
    17161 atttggtagg aaattgtcac ttgggttttt ggtactgtgt tgatttccag tgtaaaaatg
    17221 atttcaaccg tttcagcgta ttcattactt gaatattgat tatttactgg agcaatcggc
    17281 aaatcgatct ttttattgtc caccagtact tctatttctt tgacttttgg atacaatccg
    17341 gctcctaacc acataccgga tttaaatcca aatccgctac ctctgtcatt atcggtcgtt
    17401 gctcgccaaa aaactggaag tgtttcccgg tatagccatt cttttccgtt tttattgagc
    17461 gattccagtc cgcctcttgt ataagaaaaa atataatgcg tattccccga tttgacacct
    17521 aatgtacagt cgccgaagac gattgcaact ttttccgtat ccatatgact tttatttact
    17581 gtatttttca taataatatc ttacctcctg catggcgggt ttttcttgtc catctgcaaa
    17641 aacgatccca ttccctgaga attcatagtc acttggttta tcgtcaaagt caccgccata
    17701 gcgcatcact tctctgcctg tgattggatc agtcacttgg atcgcctgat ctttaaaatc
    17761 ccatatatag cctccttggt acatttcata acgatcaagc aacgtcatgt aagaatccat
    17821 tccacctaac gaattcccca tgtcatgcat atattcacat aagatgaacg gtttttccgc
    17881 tttattttca aggtagtcaa tgatctcttc tggtggcgca tacatgcagc tttttacatc
    17941 tgaaatcgaa tcttcgaatg ctggatttgg aaaattccct tcataatgga caagtcgatc
    18001 ggggtctact tttttgaagt aatcgttcat ttgtcgaatg ttttcttccg cataagattc
    18061 atttccaaga gaccaaaaaa gaatacttgg atggtttttt aatgtttcga aattcgtttt
    18121 tgctcgatca agaacagccg cctgccactg aggtactgca cctggtacgt tgtaagaagg
    18181 ctcaaatgcg cccatttttt gccatgagcc atgggattcg aggttcgttt ccgccatcac
    18241 atggattcct gcttggtcgc ataaataata ccaagtgatc tggttggggt aatgagatgt
    18301 ccgtacgccg ttgatctttg cattttttat gatagccaaa tctttttcca tatcttctat
    18361 agtgatggcg cgtccttttt tcggatgcca ctcatgtcga tttaccccgt tgataatcat
    18421 ccgttttcca ttgaagtaga tgattttatc tttgatttcg attttacgaa aaccgaagga
    18481 atagtcaata tactccgtta ctcttttttc ttcgtctatc gtctccaatt caagcttata
    18541 taaattgggc tgaaaatgcc cccacagtgc cacattttca aatccatcga tgcgaatcgt
    18601 catttgctct tttaacggat attgctgttg atagatttcc ttcccatcag gatctagtat
    18661 cgctacattc agctgactgt ttttggctac ttgctcaaaa gacaaatcta cttcaagttt
    18721 gccattttga catttgtcat ctaagcgagg tttgatccaa agatcttcta aatggttttg
    18781 tcctcgagca actaagaaga catctctgaa gatcccaaaa aatcggaaaa agtcctgatc
    18841 ttccaagaat gcagctgtac ttcttttgta aacctccaca gctagaatat tttcttcttc
    18901 caaataaggt gttaaatcaa attcagacgg agtgaacgaa tcttcggcat acccgataaa
    18961 atgaccattc aaccaaataa acattgcttg ttctacacca gcaaaatgaa tcgagatttg
    19021 ttttcccttt agaccttctt ctaatcgaaa agtcgtcaaa tacgaaccaa ccggattgtc
    19081 tttagcagct gaaaagatcc cttccgcctc tttctcaggg cttgtcgtga aagggggacg
    19141 gcgaaataca tgtccctccc acggatacat cgtattgatg taatggatct tgtcataatt
    19201 tgctaattca atatgactag gaacagtgat ttcatcaaaa gaacaggcat caaaatcttt
    19261 tttataaaat gtttttggtc gtaccgaagg atttggcgaa taacaaaaac gccatgatcc
    19321 atttaaagac tgtttaaggg aggatgtggt ttctacttct tcccttgttg cataacaaac
    19381 atgatcgcta tgtgcatttg attggttcac tctaaaaact tgtggatcat ctaaccattt
    19441 tatatttgct ttcatacttt tttccttctc tctcataact ttttgaaatg aagcagttct
    19501 gtttggaaat caccttgaaa atcggggaag taaagtccaa ctgtcatcaa ttcggccccc
    19561 gaaaatatcc gcttcgaggt ctgttcctga taaagtgcct catcatctaa ccccatcaga
    19621 tatacttcat ggaacgctgg ttgagctgaa gctagtatcc tccctaaaaa cacaatagct
    19681 tcatcttgtt taggagacac aaacatccat gcggcaatat tttcttcaaa aggactcgcc
    19741 aaacgataat aatggccata ctggatcacc ggacgaatcg tttgatagga tataatttgg
    19801 tcttttactt gctttttttc ttcaggagac agctttgtca aatccagttc ataaccaaaa
    19861 acagcgctca tagctgtttc tcctcttgtt ttcaacgaag tagatctgtc ggtctgatga
    19921 tttggtacag ctgatacatg agctgtgatg gacgaaatcg gataagcaag gcttgttcca
    19981 tattggattt tcatacgctc cactgcatcc gtgttatcac ttgtccatga ctgcggcata
    20041 taataaataa acccggcatc aaaccgtcca cctccaccag aacagccttc ccataagatt
    20101 tccggataat cagttgtcaa atgttcaagt agttcataaa gcccgagcat ataacgatgc
    20161 gctacttctc cttgtctctg tgggtcaagg gtaatcgaat agacatctga aagcgaacgg
    20221 ttcatatccc atttgatgta gtcaagagga atcgtatcta aaatctttct catttgtttc
    20281 tcaatcgtct gccgaacatc taaccttgta aaatccagta catgttgtga tctggaagca
    20341 gaaggcattc tacctggctc ctgcataaga aaatcaggat gtgttcgata cagttcagaa
    20401 tcaaccgaga tcatttcagg ttcaaaccat aaaccaaatt tcaatccttt gctatgagca
    20461 taatctgcta tttcacgtaa tccatttgtc aacttgcctt catactcgaa ccagtctcct
    20521 aatgaagaat tgtccgcatc tctttttcca aaccagccat catctaatac gaacatttcg
    20581 atgcctagct cctttgcttc atcaatgatc gccttgattt tttcactatt gaagtcaaag
    20641 taagtggctt cccagttatt gaccagaatc ggccgttcag caaattgatg acgacctctt
    20701 gcgattcttt cacgtagcaa ctcatggtgg atctgactca ttttgttcaa tccctgactt
    20761 gagtaagaaa ggatcatttc aggtgtttga aaagactggc ctgcattcaa ttcccacgaa
    20821 aaacgatggc tgttgatccc accgacgact ctcaattgat tgatctgatc tttttcaagt
    20881 tcgaaagaat ggttgccgga ataaacaaat tgaagtccga tcgcttctcc agtattttct
    20941 gtcgtatgat gatgaactaa agcaataaag ttattcatat ggtgactgct cgttcctcga
    21001 cggctttcaa atacttttgt tccagataaa acagattgcc gtgaaatctg tctttcattg
    21061 acatgtgctc ctggcaaagc aatgacttca tcaaatcgcc tagtatgggc aaagtctagc
    21121 tggaaactag ccgctttttc tagtttgatt gtttctccgg tttcatttct tatctttaca
    21181 gatcgtgtga tcactgctct atcagtaaat atggtataga acaaatcgaa ataaatcgct
    21241 gtttctctat ccttaagtga aatcaccaac gtttgtgcct ctgacttgtc catcacataa
    21301 gtttgaggca agccttcgat ctctggcttc ccatctgtaa tataatggct gtcatatctc
    21361 agatccagta agtctgatcc attcttgcgc tgaatgatcg atgcgggcgt tcgaaagtcc
    21421 atgctgccat gagaagagta ttcttgcggt agtgtatctt tcgacagaga ccggtcagga
    21481 tttaagggaa catttcctga aaatccacga tctcttcggg gataattttt attgtctttg
    21541 tactgcttga ctggtttgcc aaaataaata tgcgccaata cattgctttc ttctatcttt
    21601 atcagataag aaagaaatgt atttcgtaaa tggaagactc ggctcttttc atcaaataag
    21661 atgaaatttt tatctgtttt tctatgcgta atatccatca tttcactccc aacaaaacgg
    21721 aaaccgttta ctttttttta taattcctgt atactgttta ctaaacaatt ttgtcaataa
    21781 aaatttttta tttttttgag gagtgtgtaa aaatggttgg aattagagat gtcgcacgaa
    21841 aggcaaatgt ctctcctgct acagtttccc gtgtgttgaa taacgatcag acgatccatg
    21901 tggctcagac tactcggcaa cggatagtag aggcagctgc tgcgttggat tatgacatca
    21961 gtactcgtaa atatatcaaa aagagaatgc cttcgattgg tgtgatctct acgatcggca
    22021 aagcgagcga agaacaggat atctattaca aacatttgcg agtagggatg gaagaagaag
    22081 ctcgacgtct gcatttaggg atgaatcgaa tctataatct atctgataat cctaaacaat
    22141 gggaagatct agatcagtta ggtgcggtga tcgttgttgg gactgttacg aaagaatcga
    22201 ttaccgcctt gttaaaacaa aacatgcaaa tagtcgtcgt ggataaccct gacatccagc
    22261 aagaggtcga tatggtgtat gcagatcttg aacggatgac taaaaccgta ctggaattgt
    22321 tcttagaaaa tggtcataaa agaatagcct atatcggggg atataaagtc gatatggatg
    22381 aattcggtgg gaagcagatt tctataaatg aaaagaggat gcgtgcctat cagcatttta
    22441 tgatcgatca tcacttagaa aaatatatgt cttatcatct aggtggctgg acagaaaaac
    22501 aaggtgaggt acttgcgact aatctattag aaaatgaatc ccctcttcct actgccattc
    22561 ttgtcgggag cgatccaatg gcaattggtg tctatcgggc attgaaacaa aaaaatatcc
    22621 aaattggaga agagattgtt gtagcaagct ttgataatat cgagacaatc gcaaacctct
    22681 cccctggctt gaccagtgtt caaatcaatg cgaaatcaat tgggcaagca gctgttcgaa
    22741 tggcattgga gcgaattgac ggtatacggg acgaaccaat cattttgact tatcctgcac
    22801 gattgattgt tagggaaagt tttatcccaa gaacaaaaag gtattgaaag agaggttgtg
    22861 aaaagccgct tcgtttcttc atactcaaaa cgaaacggct ttttcacaac ctcttactta
    22921 tacttattca caaaatccaa tgtctgctgg taacaatttt gcgattcttt tagcgtgtaa
    22981 tcaaattggt attcatgggt aatctctttt tttgtatctg tataaaacaa ggattgcacg
    23041 ggtaccttta attctgtcaa ccgattttct aatgcttgac cttgctcctg gaatgagtat
    23101 gcatttccgt cagttatata cgttggcgga aattccttag aaacatggtc agccacacta
    23161 gcttctttca attgctcact cgttttccaa tcttttgttc caatcaacga ccacgcaacg
    23221 gttttgacga aaaacttcat aaatcgatca tccgaatgct gctgtgctgt tagcttcaaa
    23281 tcaacaggtc cgcaataaga aatggttcct ttcagactat cttttggaag tagctgtttg
    23341 attttcattt gtttagcata cgcatcattc gtttgtatag ctgcatattg caatgcgatt
    23401 tgtgaacctg cactatctcc accaaaaaat aattgttcca tgtctagcaa tttgtcgtct
    23461 ttttcttcag ctaataaatc tttgatcagt tcattcactt gaagaacttg attagggtat
    23521 tctgaatcag gcgctaattc ataattcatc gcaaccactg cgatgttggc gtctgaaact
    23581 aattttgtcg caaattcttt gacacccgat ttatctccgc cgacgaatcc tccaccatgt
    23641 acccaaaata gaacaggaac aggccccttg ctgttttttg gatagtagat gtcataagta
    23701 ttctctttat acttggactc atacgtcaaa tctgtttttt tcgccacgtt tttttctgct
    23761 tgatcgaacg tttttttatc agtgatttga acagtatttg aaaacatatg gttgatcaag
    23821 aatgctccag gtctggggga aagctggaag gcaagaaaga ttgcaaacgc gatcccaaga
    23881 atgattccga agaaccatcc gataaattta agaattttct ttttcataag gctccctcag
    23941 tagttagtat taagcattct gattatacca tagtgttatt tcctatcaag ctctcctaga
    24001 tgatttaatg attttcccca aacttcttct gcaatgcttt tgacaaaaga aattttttcc
    24061 attgttcttc aaacaaaaca ttttcttctt ctgttgaagc aaatccacat tgaggactta
    24121 agcacaaacg atccaaaggc agatatcgtg tcgcttcgta gatccggtca atcacttgct
    24181 ggtgctcttc catttcgcca tttttcgtta cagtcaaaat ctaagacaac taatttatct
    24241 tcagagactt tctctaaaag aacaaaactc ccagcacgtt cactatcgta ttctaaataa
    24301 taggcacgga cattttcttg agtaaaataa tgggtctgct acgtcgtcat cccccctgat
    24361 gccgccaaaa tcgaataata atttccgcgt caaacatgta tatttagtgt tgaatcattt
    24421 gagaatatga aaataaaaat ctttttgatc aatcagttaa tcaaattgaa ataacgaaaa
    24481 atctgagaca gaatgacgtt cattctgacc cagatttgat tttgcacaac tcaacagtcc
    24541 ttattggcaa atcgccaaaa taatgtagac taacta
//
LOCUS       DAZKKW010000039        24220 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.039, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000039 DAZKKW010000000
VERSION     DAZKKW010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24220
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.039"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(88..1383)
                     /gene="purB"
                     /locus_tag="ACG8PO_001488"
     CDS             complement(88..1383)
                     /gene="purB"
                     /locus_tag="ACG8PO_001488"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFQ0206806.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(1396..2520)
                     /gene="purK"
                     /locus_tag="ACG8PO_001489"
     CDS             complement(1396..2520)
                     /gene="purK"
                     /locus_tag="ACG8PO_001489"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFQ0206807.1"
                     /translation="MVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVSDCP
                     AAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTDLLA
                     ITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKGQYV
                     LKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHNNIL
                     HETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRPHNS
                     GHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENKPDW
                     QFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            complement(2513..3001)
                     /gene="purE"
                     /locus_tag="ACG8PO_001490"
     CDS             complement(2513..3001)
                     /gene="purE"
                     /locus_tag="ACG8PO_001490"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFQ0206808.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            complement(3417..4724)
                     /locus_tag="ACG8PO_001491"
     CDS             complement(3417..4724)
                     /locus_tag="ACG8PO_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleobase:cation symporter-2 family protein"
                     /protein_id="HFQ0206809.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMILISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            complement(4727..5308)
                     /locus_tag="ACG8PO_001492"
     CDS             complement(4727..5308)
                     /locus_tag="ACG8PO_001492"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA];
                     GO_process: GO:0046110 - xanthine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFQ0206810.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     regulatory      complement(5424..5521)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            complement(5880..7172)
                     /locus_tag="ACG8PO_001493"
     CDS             complement(5880..7172)
                     /locus_tag="ACG8PO_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFQ0206811.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     gene            complement(7169..7792)
                     /locus_tag="ACG8PO_001494"
     CDS             complement(7169..7792)
                     /locus_tag="ACG8PO_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0206812.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            complement(7795..8631)
                     /locus_tag="ACG8PO_001495"
     CDS             complement(7795..8631)
                     /locus_tag="ACG8PO_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0206813.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            complement(8648..9535)
                     /locus_tag="ACG8PO_001496"
     CDS             complement(8648..9535)
                     /locus_tag="ACG8PO_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0206814.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            complement(9551..10834)
                     /locus_tag="ACG8PO_001497"
     CDS             complement(9551..10834)
                     /locus_tag="ACG8PO_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0206815.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVNKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            complement(11517..12200)
                     /locus_tag="ACG8PO_001498"
     CDS             complement(11517..12200)
                     /locus_tag="ACG8PO_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFQ0206816.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            complement(12218..12835)
                     /locus_tag="ACG8PO_001499"
     CDS             complement(12218..12835)
                     /locus_tag="ACG8PO_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFQ0206817.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            complement(12845..13669)
                     /locus_tag="ACG8PO_001500"
     CDS             complement(12845..13669)
                     /locus_tag="ACG8PO_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HFQ0206818.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            complement(13662..14222)
                     /locus_tag="ACG8PO_001501"
     CDS             complement(13662..14222)
                     /locus_tag="ACG8PO_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0206819.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            14396..15352
                     /locus_tag="ACG8PO_001502"
     CDS             14396..15352
                     /locus_tag="ACG8PO_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFQ0206820.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            complement(15657..16613)
                     /locus_tag="ACG8PO_001503"
     CDS             complement(15657..16613)
                     /locus_tag="ACG8PO_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HFQ0206821.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            16717..17319
                     /locus_tag="ACG8PO_001504"
     CDS             16717..17319
                     /locus_tag="ACG8PO_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFQ0206822.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            complement(17407..18906)
                     /locus_tag="ACG8PO_001505"
     CDS             complement(17407..18906)
                     /locus_tag="ACG8PO_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HFQ0206823.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(18922..19446)
                     /locus_tag="ACG8PO_001506"
     CDS             complement(18922..19446)
                     /locus_tag="ACG8PO_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFQ0206824.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            19744..20493
                     /locus_tag="ACG8PO_001507"
     CDS             19744..20493
                     /locus_tag="ACG8PO_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFQ0206825.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            20484..21269
                     /locus_tag="ACG8PO_001508"
     CDS             20484..21269
                     /locus_tag="ACG8PO_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFQ0206826.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            21238..21954
                     /locus_tag="ACG8PO_001509"
     CDS             21238..21954
                     /locus_tag="ACG8PO_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFQ0206827.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(22056..23075)
                     /locus_tag="ACG8PO_001510"
     CDS             complement(22056..23075)
                     /locus_tag="ACG8PO_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HFQ0206828.1"
                     /translation="MRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIVSAKDH
                     DAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVPSYSPN
                     AIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRIGLTEA
                     VLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQMINEG
                     FLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSFKPWET
                     IPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNEVSLPK
                     ARQLR"
     gene            complement(23094..24095)
                     /locus_tag="ACG8PO_001511"
     CDS             complement(23094..24095)
                     /locus_tag="ACG8PO_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HFQ0206829.1"
                     /translation="MTSTVFIILLGFVCRKKGIFSESVGKILSKVVLTVALPALAFRA
                     FMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYKGDKQTTLRVLTIFGSTTFF
                     GTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGLKMEAKNIKAMFVNPIIIAT
                     FAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPWLFQPMDYLAGLSSPLAWLA
                     IGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILAVLTATNILPVNFEALATIV
                     IMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVLTPVWIVVLQMIHGIGLFS"
BASE COUNT         7171 a         5222 c         4226 g         7601 t
ORIGIN      
        1 aaaagaagca gaaccgctaa cggatctgct tcttttttgg ttatttttgc tattctattt
       61 taattgaatt ttgcaactca ctcttcttta ttccaaacct actcgtttga atatcacgtc
      121 aacgtttttc aaatgatgat gataatcaaa ggcatcgtct atttcttctt tagataatac
      181 cgatgtgatg gttggatctt cttctaacag cgggcggaac tgcacttggt tgtcccaagc
      241 gtacgcagtt tttggctgaa caagatcata agcttcttct cttgtcattc ccttatcgat
      301 caatttcagc ataacgcgct ggctataaat caaaccaaat gttgcagaca tattccgctt
      361 catgttttca ggaaagaccg ttagattttt aacgatattg ccaaaacgat ttaacatgta
      421 attcaataaa atcgtggtat caggcaagat gattcgttct gctgaagaat gagaaatatc
      481 tctttcatgc cataatgtga cattttcata ggcagtgacc atgtgtccgc gcaccactct
      541 ggctagacca gccatatttt ctgaaccgat cggattcctt ttgtgcggca tagcagaaga
      601 acctttttga cctttcgcaa aaaattcttc tacttcacgt gtttcggatt tttgcaatcc
      661 gcgtatctct gttgcaaaac gttcaatact tgtagcaata agcgccatcg ctgagaaata
      721 ttcagcatgt agatcccgag ggagaacttg agtagagatt tcttgtggac ggatccccaa
      781 ttgtttacat acatactctt caataaatgg cgggatattt gcgaacgttc ccactgctcc
      841 tgagattttt cctgcttcta ctccttttgc cgcatgttca aatcgttcga tgttccgttt
      901 catttctgaa taccatgtgg caagtttcaa tccaaaagta gttggttcag catggacacc
      961 gtgtgtccga cccatcatca ctgtatattt gtgttctttt gctttttcgc caatgatttc
     1021 cgtaaaacgt ttcaagtctt cccgtaaaat atcatttgct tgcttgatga gatagccata
     1081 agcagtatct acgacatccg tacttgttag accataatgg acccattttc tttcttctcc
     1141 caatgtttca gaaaccgctc gtgtaaacgc aacgacatca tggcgtgtta atttttcgat
     1201 ttccaagatc cgatcgatat caaatgatgc atgctcacga atttttttca catcttcttg
     1261 cgggatttca ccaagttctg cccaagcttc atctgctaaa atctcgactt cgagccacgc
     1321 acggtatttg ttctcttccg tccagatatt ccccatttca gggcgtgtat aacgttctaa
     1381 catgtttttt ctcctctaat cccaaatatt cgtttgtttg atttcttcca gcgtttgctg
     1441 gatgtcttct gtcaagatag tgatatgtcc catcttgcga cctttttttg cttgtttttt
     1501 tccatagaaa tggaattgcc agtctggctt attctcaatc aagcgatagc tttccagcag
     1561 ttgttctccc aaaacattga ccattactgc attgcttatc aaacgtactt tttctcctaa
     1621 tggccaacca caaatacctc gaatatgggc atcgaactga ctcatagaac aagcttcaat
     1681 agaataatgt ccagaattgt gaggacgagg agctaattca ttcacatata gcccgcctgt
     1741 ttttgttaaa aacatttcta cccctaaaac acctgaaagg ctcactgctt ctgcaatcac
     1801 acgggcaata cgctcagcct cctcgatcac atcctgatca atcgcggctg gagcaatcgt
     1861 ttcatgaaga atattgttgt ggtggatgtt ttccacgacc ggaaaagtcg tgaaatctcc
     1921 ttgaccattt cctgccacca tgatcgaaat ctctttttca aacgggatcc atgcttctaa
     1981 aacacaggtt ccttctctta aaaggtccat ggcaggagct agatccgccc gacttttcaa
     2041 aacatactgc cccttgccat catagccgcc tcgagtggtt ttcaaaacac aaggatagcc
     2101 gatcccgtca atcgcatctt ggatatccgt cggactgacg atcgttgcat aaggagcaat
     2161 cacgatgtta ttcgtttcca aaaaagattt ttctaacagg cggtcttgcg taatcgcaag
     2221 caagtctgtc ccttgaggaa taaatgacat gggtaaaatc gcatttaacg cctctacact
     2281 gacgttttcg aattcatagg taatgacatc tgttcttctg gcaagctctt tcagcgcaaa
     2341 ggtgtcatca tagacacttt caatgtgcca atctgcaact tgtgcggctg gacaatcgct
     2401 cacaggatcc agcacaccta ctttaaagcc catttctttt gcactaatcg ccatcatacg
     2461 acctaattga ccgccaccga cgatcccgat cgttgaacca ggcaacaaag gtttaaccaa
     2521 gttgatcact actttctata acagttttag ccaacattga acgtctttca gccaatttag
     2581 cctccaattc taaatcatac atggaaagca tctgtatcgc aagtagtcca gcattaattg
     2641 ctcccgcttt tccgattgct gtagtagcta caggtactcc tccaggcatt tggacaatcg
     2701 aaagaagcga atccaaacca tttaatgttc gtgattggac cggtacaccg ataaccggca
     2761 aagttgtttt tgatgcgacc atacccggca gatgcgcggc tccccctgct cctgcaataa
     2821 tgattttgat tccttttttt cgagcgtttt gtgcatattc aaacatcaaa tctggggttc
     2881 gatgagcaga aacgaccttt ttttcataag acacaccgaa ttcatccaat atttgacatg
     2941 cttctttcat tgtttcccaa tctgaagtac tccccataat cacagagatt tttgctgtca
     3001 ttgtttttcc tcccagtttt tctacattca tattttaaca atcacttttt ttgatgtcaa
     3061 agaaatttcg aatgttaaca aagaaaaaca ttttattgtt cgtgtttatt aaagataaaa
     3121 aaaaggaatg tgacaaaagt taggtcacat tcctttttat ggataaacgg tgttctcctg
     3181 ccaactcctc ggtcacaagg aacaatattc aacaaacatg agaagctttt ttttgaaatt
     3241 gttccttgtg actcggagat gaacggcagg ctcacgacct cttccttcaa cggtgttcaa
     3301 tcagctgacc ctcggtatta gctcaaacaa acggcaaaat acgaggaact atttttccgt
     3361 ttgtttctct aacaatcagg tcaaaacgct gatttcacaa cctttctttt gctctattac
     3421 ttcttgaagg cttcttccaa tatttcttca tcttttttca atccattaaa gatcaaattc
     3481 aatatgatag ccgtaatgct actcattacg atgccatttc cagtgaacat ttgcaatgtt
     3541 tgcggcattt gactgaataa tgtcggcgta acattgaaac ccagcccaaa tccaacagaa
     3601 acagcaacaa tcaatagatt tttatcattg ctgaaatcaa cttccaacag catacggatt
     3661 ccttggactg ccaccatccc gaacatgact aacattcctc caccaagtac cggctcaggg
     3721 atgatttgtg ctaacgcacc gattttaggg aataatccta aaacaatcaa gaagaatgca
     3781 gagaaataga tgggttttct tgttttgatt cccgaaagtt gaaccaatcc gacattttga
     3841 gaaaagccag tataagggaa tgtattgaaa ataccgccta agataacggc aagtccttct
     3901 gcacgatatc cctttttcaa ttcttcttct ccaacatttt tcccagtaat gtcaccaagt
     3961 gcaaaatata cacctgttga ttctaccatg ctgaccatgg aaatgaggat cattagtaaa
     4021 atcgaccata cgtcgaaagt tggtttacca aaataaaagg gttgtggaac atggaacaac
     4081 ggcgcatctc caatcgcgct cagatcaatc attcccatga aagcagctaa gatgcttcct
     4141 ccgactaaac caatcaatac cgcgattgaa cgaatgaacc cttttgccag catttgcacg
     4201 cctacaatta ggagaatcgt cacaaatgca agtagcagat ttgttttgtc accaaaatca
     4261 gctgctgttg cattacctcc acccattttc tcgattgcta caggaatcaa agtcaaacca
     4321 ataacggtga taaccgttcc tgttactata ggagggaata gttttttaat tttagaaaag
     4381 acaccagaaa ctaacacaac aaagatccca gaagcgataa tagatccgta gatagctcct
     4441 actccttcat tggttccgat caagatcagt ggagctactg cttggatcgc acatcctaaa
     4501 acgacaggca agccaattcc gaaaaatcgg ttaactgtca attgcaataa tgtcgctaat
     4561 ccacacataa agatatcaat tgaaatcaaa taagtcatct gttcttgatt aaagcctagt
     4621 cctgtaccga tcaaaagagg caccgctact gctcctgcat acatcgctag cagatgctgc
     4681 agccctaaaa ccgcagcttt tccattttgt gtttcttttt ccacgattat gcgtcctcct
     4741 caagaaactc aacttgtcca ttcgtcaagg aagcaatacg cgcaagagaa acgactctca
     4801 atcccatttc ttcaagtaac tgacgtccat cttggaaaga cttttcgatg acgatcccga
     4861 ttccttctac ttgcgctcca gcttgctggc ataattcgat caatcctttt gccgcttgtc
     4921 cgtttgccaa gaaatcgtcg ataatcaata ctttgtcttc tgaagataaa aatttacgag
     4981 aaatagaaat cgtacttgtc acttgtttcg taaaagaata aacagaagct gtcaataatt
     5041 cttcatccat cgtcaaactt tttgcttttc tagcgaagat cattggaaca tctaattctc
     5101 ttgcagcaaa taaagctggt gcgatcccag aagcttcaat ggtcacaact ttcgtgattc
     5161 cttgttcttt aaatacttct gcaaaccgtt ttcccatttg ttccatcaat acagggtcta
     5221 cttggtgagt gacgaagctg tcgactttta atacgccctc gcctaatata cgtccgtctt
     5281 tttgaatacg ttcaactaat tctttcactt tatttgctcc tttatcattt attaataccg
     5341 aaataaccgc ttttgtgcaa aaacctacta tgtttctgtt aaagaaaaac accctccact
     5401 ttatcataag aaaagaaggg tgaaatagcc aatcttttac ttatagtcca gcatttacgg
     5461 tactggctag aaactgtcat ccatattatg acgatatata agtaggtatg taaaactaat
     5521 aatagtgtaa attcattttc ttgcaactgt tttgcacgtg aaggcagttt gccatatttg
     5581 aataaaaaat gttgtttcaa atattgaaaa cagtctaaca ctactttctg aaactgacaa
     5641 cgtggtttac ttattttttt gtaaaatcta atgattttta atgcattatt cctttttgtt
     5701 cgggtttaaa tagttatttt tattttctcc tatctaaaaa agcagcccac ataaaaagaa
     5761 ggcatgacaa aaagcatgtc atagcccctt tttccgaata aatagtgttc aaaagacagc
     5821 ctatcggcaa taaggcaaga ttttataaac tacgggaagt aatttccgta aatctattct
     5881 tattctttta agctgacaag ctcgtcaatc actactgctt ttcctgaagc aatagaatga
     5941 tttgctgcga ttcctgtcaa aatcgacaaa ataccatcta cgtgagttgc tgcccgattc
     6001 cacggatctt cttctttttc ttcaccaaat agatcatcta ataaacgcgg atctcctccg
     6061 ccatgtccgc ccgcttcttg ttcgacgatt acctcatatg gctcaccgaa catcggatag
     6121 acatggatgg actgaagaac ggctgctcct tcatcctttt tatctccacc tgcattgatg
     6181 taagactttt cgatcacttt catttcgatc cgtcctttgc tgccattgaa agaaacattg
     6241 aatccttccc acggcatata cgcgtttagc gaatacgtca aaatcgcacc agtttcatat
     6301 ttgaccgtta cgcctagtgt atcttcaata gagatatcat cggaaaatac actgcggtca
     6361 cgaatatatc cactgtcttt ttctgcttct aaatacattt ctcgcaattc ctcattggct
     6421 gacaggtcta tagcaaatgg atcagcttta gctagttcgc ttccatagca tcgactgtaa
     6481 aatatttcta ctccgcgttt ctccgcatta tgcatcccat aaaagtttaa tccaccgaat
     6541 gcaaatacct tttggggacg gctgttcaac caaaaattca ctagatcaaa atgatgggta
     6601 gatttatgaa caagaagccc gccgctattg tgcttatttc gatgccatct tctgaaataa
     6661 tctgccccgt gttgcgtatt taacagccat tcgaagtgga tagaaaacac atcaccaatc
     6721 acatcacttt ctatcagttc ctttatcttc gtgtgatacg gtgcataacg ataattaaaa
     6781 gtgactcgca ggcttcttcc cgtctgtttt tggaccgtta ttatttcttg agctttttcc
     6841 tcatcgatag tcagaggttt ttcgcaaata acatcatatc cttttttcat tgcacgaatg
     6901 atataatcat gatgcgttcg atctactgac gtaataatga cttcagccgg ccgtacgtca
     6961 tccagtagtt gctcaaattg tgatggcata catgtcttta cttttttctg tccatacttg
     7021 ttcacaagta tccgatttgc ataatccatc cttgtttgag atacatcaca aaaaccgact
     7081 agttcacaag tttgatgata cgtagaggca attgcctcat aaaaaaaacg cgctctccca
     7141 ccaagtccga cttgtacata ttttttcttc atttaaagtc attctccttt tcttttaagc
     7201 aaaatactgt gacaaatggc agccctggat acagtaatgc agtgagtaaa aaatcatgct
     7261 gtatacacca gaacagtcca tacaaaaggc ataaatacca cagaaacccc ttgacgtttt
     7321 ttgacaacag ccagatattc cttttgatca aataaacaag actatctgct ttatcttctt
     7381 tgcagatatc aattagcgta ctaaaaaaac tatgggatac aaaaagcaac agacacaata
     7441 gaaaaggaat cagataccaa gctccaatct ttcccactaa taaataccaa tcaactgcaa
     7501 aaataatgac aaacagctga aacaacgtaa tgatgaaaca catccgtttg atttttcctt
     7561 tcctccaaca tacctttttt tctgccaagt aatggacaga tagataataa gaaggataca
     7621 tcatcaaaaa ataaatcaga tagacaacac gagcagacag cactaatcct aaagtgaaca
     7681 aacatcctag aactaaagcg aaaagagcat tactgataag actggttaaa aaaatccgac
     7741 agacatagta gttgagcttg taaagaaaca tctcttctat tggctttttc atattcagcc
     7801 cttcagaccg cttgtactga tcccctggac aagatattta ttgaagaata agaaaatcag
     7861 aaaaaccggt agcagcgaca acgtagacat cgcaaacata ctcgcgtaag tcgtactaga
     7921 tccagcgtct gcaaataatt tgatggcaat cgataccgtg taagatctcg gcttgtttaa
     7981 ataaatcaat ggtcccatga agtcgtccca tttccaataa aactggatga tgatcgtcgt
     8041 gatgatcgaa ggtttcagca aaggaaaaat aattttggta aagacactat acttgctgca
     8101 tccgtcaata atcgctgatt catctaactc tttggggatg ctgaccatga attgcatgat
     8161 ctggtaaatg aaaaatgctt gtccaaaaaa atgtggtaaa atcagcggca catacgtccc
     8221 aaccaaattc agccgattgt aaataataaa ctgcggaatc aaaatcactt gtgcaggaat
     8281 catcatcgtt actaacatga cgacgaacca gaatcttttt cctttgaaat cgattctcga
     8341 taatgcgtaa gcaatacaag tcgagctcag aactgtccct atcgtcgaaa ctgcagagat
     8401 gacaaacgaa ttcctaaaga aagtaaaaaa tgaaattcca gcaaaccccg cccatccttt
     8461 tgcgtaattt tcccattgaa aagtctctgg aatcaaattc agcgaaccac tcataatctc
     8521 ttgatccgtt ttaaaagaac ctgcgatcat ccataagacc ggataaatca tgactagacc
     8581 taatagcaaa ataaaaccgt attgaaacca tttcgttact gtattattca tttcttcctc
     8641 ctctcattca cacaacgacc catttttttg atgatttgaa aatcagaaag gtgacagctc
     8701 ccataatcaa cagcatgatc cacgaaatag cactagcata ccccatagaa ttaaaagaaa
     8761 aagcttggtt atacacataa agagcataaa agttcgtggc atcattcgga ccaccttggg
     8821 taatgataaa tgcctgcgta aaagtcataa acgccgcgat tgtctgcata accaagttat
     8881 acaaaatgat cggcgaaaga agaggtaacg tgatcttgta aaacgtctgc catttgttcg
     8941 ctccatcgat ctctgccgct tcataatacg agcctgggat ctctttgata cctgcagcaa
     9001 agatgatcat tgccgaaccg aattgccaag cagacatcaa aacaagaggg atcatgacca
     9061 gtttttgatc gctaaaccag gaaagcgcag gtgcgcctaa tcgttccagg aaaatattca
     9121 ctaacccgtt tttagaaaac aactcccgcc aaactagagc gatagaaata ctgccaccga
     9181 tcaaactagg cacataatac aaagaacgaa agaaagtcgt tcctttgata ttcctcgtta
     9241 aaagataagc aatcaataga ccaaagatca gtttcacagg tacagatgca aatacataga
     9301 gaaaagtgac tcggatggag ttcaaaaaac gggcatcact cagcatcttt atataattat
     9361 ccaagccgat ccatgagact ggatttccca aacgataatc ggtgaacgaa taataaagtg
     9421 acaagatcat cggtatcact gttatcaata gaaatcctag gatgaatgga gaagcaaata
     9481 cgtaaccgac cacattgtct tgatccaaga accgttttag tttttctttt ttcatgcctt
     9541 cctcctgctg ctatcctaga atgtttgacg caaactcata aagactctct gctgcttctt
     9601 ctgctgttac ttcttcataa atgacttttt caactaaaag attgtattgg tcttgaattt
     9661 ccggattcaa agcactatca aagacattca cttcgccgtt gtccatatcg gaaatcattt
     9721 ctaaataatc aaatgtcgct tgcaaagttg catcatctgc acgagcgagc tgttctcgaa
     9781 tattcgacat gattggtaca ccacgttctc cttttaaaat ttcattggct tcttcactgt
     9841 tttgaaagaa atcaataaac ttagcagctt cttctttatg ggctgagctt tttgccatac
     9901 tcagcatttg cgaagaacgt aaagggatac cagattctcc atctgctttt ctgcgaggtg
     9961 gattaatcaa ctgaatctcc atatcttcag gagcccctga caatatttct gtcaactggt
    10021 tacttgatag aaacaccatt gccgcttctc tatccctaac cgcctgaaaa gacagatccg
    10081 tcgtacttgc agctccacca cctggttctg gatgggcccc tttgtttacc agatttttac
    10141 gcagctggaa aaaatctgtt agatagtctg gattttcaaa tcccaagctg ttagatagat
    10201 cttcccgatt ataaaaattc aatcctttct caacttgtgg tatttgaata atacagccag
    10261 ctataaaatc ttcgaatctt gaaatagcat agattcctaa ttcttcagtg atttgatagc
    10321 tgatttgttc aaattcatcc catgtccaat ttgaagctgg ttcagggata tttaattgtt
    10381 caaatatatt tttattgtaa gcaatcgcaa acgaattcac cccgttagac agtccaacta
    10441 attgcccgtc gtaagtcgtc gttgctaaat aggattcatc tgcattagaa atatcaatta
    10501 tcccttgctc cacatattca tctaatggtt caatctgatt gatgtattga ggaaattgat
    10561 tgttcagttg gaaaatgtcc cagatatcat ctgatgctgc tagtgtacct aattttgtgt
    10621 aatatccttc ataatcataa aattcataat cgatcgtcac atggggattt ttttcttcat
    10681 aaagctcgat tacttttact gttgcatcgt gtcggacttg cgaaccccac caagccatgt
    10741 tcagcgtgat ttttttgtca tcttcttgcc cacaagccgt caagaagaat aacataacac
    10801 ttaaagctaa gatgattctc attcttttct tcattctctg cacctctact tccattgtta
    10861 tttttcaata tactgtattt ttatacagaa catatttatt aaacgatctt tttcaagggg
    10921 aataaaaacc aagacttgcc ttatcgttct gcctaggatg aacaactcaa tactttcaag
    10981 aaaaattaga tagtgctgat tcttctttca tcgctaaaaa cacttcttgc ttcaatgatg
    11041 ggaggataat tacgcaaaga aaaaaggata tcagacaact gcccaatatc cttgacatag
    11101 ctaaataagc atgcttcttc gtgtgcgctc ttccctaaaa aacagaaaaa taactagtaa
    11161 ttctgtatga aattaggtac aatatacacg tcgatgcaca agcattgact atggcaactt
    11221 gattggtcag ttcccgctga cttttcaagc cccgacaatt tagctgctac taaattgtcg
    11281 ggtgcctttt ttagttttta ttcgtttttc attctataga aagcgcttca ataaatcaag
    11341 acattttttt gttagaatgc taataataac aaaaggtttt aactcttctt atgtacatta
    11401 caggtaaaaa aacaaaaaaa tttctctttt ttttgaattc tgaacaaata gtactggagt
    11461 acaaaagacg aggaagagcc actatctgct cttcctcgtc ttttgattct cacctgttat
    11521 tcatgattct ttttctcagt ttcccgatat ttttccattt tacgactaaa aagctctccg
    11581 ctacttggtg gcgtatatgt gatggcattt gctcctgctt gaatggtctc agcaatactt
    11641 tcttccgttg gaccacctgt cgcaatgata ggaagttcag gatatttttt acgaaaatgg
    11701 cgtactgctt ctgcagtttc tttacctgca ctgatattga taatcgaaat accagctgct
    11761 aacttttcat ctatttctgt atatttcgac gttaccgtac tgataactgg aatatcaact
    11821 actcgacaaa cttcttttac tgtctctaaa ggcgtaggac cgttcaaaac gacccctaac
    11881 gaaccttgcg cttctgcgaa catgctcatg taggaagaac gagcaccttg tgtcaaacca
    11941 ccacctacac cggagaaaac tggaatgtca gctgcctcaa taatgctttt agtaatcgct
    12001 ggatgaggtg taaaaggata cacagcaatt actgcattcg catttgtatt acgtatgatc
    12061 gcaatatccg tagtaaagat gatcgaacgg atcaattttc caaatatctt gatcccgctt
    12121 gcttctaaaa tgatttccgg tactcttacg atatcttttc tcaattccgt catgatttct
    12181 ggcgtaaatt gttctgccat cagccacttc tcctttgcta tctccggtca tactgattca
    12241 attgatacgt ctcgatgacc tggatgattt tttgcgcata accggggtct gtcgcataac
    12301 ctgcatcttg caatgcttgt gcggcttcct gatagttggt ggccgtaatc actttttcat
    12361 acagcgcagg attccaatct actccttgga cgaacagttg tgtatggtca tccatggact
    12421 gttcccatga atcatacact ttgaagtctc cttggatagt tatccattct tcgttaacaa
    12481 attcctttgt ttccaggctt actttcttct gatccccata agccttgatc ccgaaaagat
    12541 tattgtattt gctagccaag gtactttttc cccaatttga ttctaatata gcttgtccta
    12601 aaatgatact aggaagcact ccataaccat cttgtagttc ttgggcatgc ggaacaagcc
    12661 gttcaataaa ttgctgatgg gattgcgctt cattttcttg ctgattatca gtaaaaggag
    12721 aagacagatt cttcaaggaa aaaacaaagg ctaagagtat cactgctaca ccggcaacca
    12781 ctgctggcca acggatattc ttgcgctttt gctttttcca cttttttttc gccatgatca
    12841 attcctactt ttcatttaat tcattcagat attcttctag cgtcaattgg tgtttggtga
    12901 tatactcagc gttttcttta ccgacataac gtaagtgcca aggttcatat gtgatcttag
    12961 ttatatcttc gtgtcctttt ggataacgaa cgataaatcc atactgcgca gcattatctg
    13021 cgatccattt agctccaggc tgatctccgt aacttgcatc caatacagta gaaggataac
    13081 tgttgtacca gttttgatcg acgacatcga ctgccagacc tgtgtggtgt tcgctgtatc
    13141 ctggctccgt aatcgtttct ttagtaatct ttgtcgcctc ctcttctgat acaccttgac
    13201 gactcatcac atcttggaca ttttgttcga aaacttgttg ttgggatgat acagaccggt
    13261 aagctgagac catcactaat ggaaatccag ctgcttcagc agccttggca aatgcttcat
    13321 attctggagc aatgcgtttg tcgatcaagt atccgttact taatgatgcc aattgagtag
    13381 actcatcaat ttctttttct aatttgtgat ctggaccaac caaaactagc ttccaatcgt
    13441 ctgttttaac atctggcaat tgtttggacg tacgatcttt tgtttctttg tcctttacct
    13501 tgctgctggt tgtttgactg ttcttttctt tactagcagt tgttttggtg cttgttcgtt
    13561 cgactgcttg attgccggct tgaacatgcg aatcatcatc gctaaaaata acatagccca
    13621 ttgctgcgat cataatcaca actgctccaa atcctaaaaa tttattcact tgaaaaatcc
    13681 tttcgttgtt cttcacgttc cccattcgcc tcatgaacgt aagtttcttc cagattttca
    13741 ttcacccaat cgagaagttc gattttttct ccttcgatct gtttttgaac atctgctggc
    13801 agacgaaaat tcaaaatatc atcatagccc cattcatcat attgaatact tactttcaag
    13861 tcaaggtatc taccagcctg ctgattattt ttcaaaggga tcagctcgac ttttgctgct
    13921 ttgctgctaa gccatttttg cgcgatgcgt gttttgattt gcttgtactc tgcaacggca
    13981 acgtttcgtt tttcgggaaa aataatcgtt tgacgcaaaa tcttttgcgt tagcatccat
    14041 tcataatcag tgaaaagatt ttttactttc tgcatcgctt cagatggcag atttttctcg
    14101 ccatctttcc atttttgcca ttgttctgga ctgatcagca agtattcatc taaaaaattt
    14161 ttttcagttt caaactgttc taataataca ctcataataa gatcaatata tacttgatgc
    14221 acgtttatcc cctcctttta actattctac ttaagttttg atcaaaaagc acctcttaaa
    14281 tcgctgcttt catttttttt aatgtttcaa gtcctctcct ctttattctt cgtgattttt
    14341 gttataataa tagacagaca agaaactcaa tccaactatg aaggagcgtt ttcagatgaa
    14401 aaaatatcaa tggggaatcg ttggtcttgg taccatcgca catgagtttg ccgaaagctt
    14461 caatcaagag acgagcgaac taacagcagt tgcttccaga acttcagaaa aagcggaaaa
    14521 cttcgctcac cgttacaata ttccaaaagc ttacggaagc tatcaagaaa tgttagacga
    14581 tgctgaaatc gacatcgtgt acattgctgt accaaataga cagcatatcg accatatctt
    14641 ggcagcattg gaagcaggca aacacgttct atgtgaaaaa gccatcacga tgaataaaaa
    14701 agaactggct gacgctatgc gcttagcaga agaaaagaat ttgatcttag cagaagcgat
    14761 gacaatcttc aacatgccac tttatcagca gttacgctca ataatggata caggaaaact
    14821 gggggcttta aaaatgatcc aagcaccttt cggcagttac aaagaaccag accctaaaaa
    14881 ccggttcttc aatccagaat tagctggagg agctctgttg gatatcggta cgtatgctgt
    14941 atctttcgct cgtttctttt tgagctctca acctgaagta gttgcctcta caatggttcc
    15001 ttttgaaaca ggcgtggacg aacaatccgt cactatcttg cgtaacaaag aaaatgaact
    15061 tgctgctgtc agcttgactt ttcaggcaaa aatgccaaaa gtcggtatcg ttgcctttga
    15121 gaacggttat gtgacgattg ctgattaccc gcgtgctgat cgtgcggaga tcttatttac
    15181 agatggtaca aaggaattca tcgaaagcgg gaatacatct gaagcaatga attacgaaat
    15241 aaagaatatg gtacacatga ttgaaggtga cttgcccaat cgctctctct tcttgacaaa
    15301 agatgtcatc gaaatcttgg atcaaatgca agttctatgg aaaaatatgt aattttaaaa
    15361 ggttgtgaaa aagctgttct gcatcaagta ataagaacag ccaaacaaaa atagctcctc
    15421 ctatttttcg tttggctgga cttattaccg cagaggaaag cttttgaaca ccgtttaaga
    15481 aagaggttgt gagcctaccg ttcagctccg agtcacaagg agcaatttca aaaaacagct
    15541 tctcatgttt gttgaatatt gtgacttgtg accgaggagt tgacaggaga acaccgttta
    15601 aagaagaggt tgtgaaatca gctttttgac ctgaatatta gctgaattca caacctctat
    15661 ttttggattt tttgttggga gaattctgcc ttgatttcaa taaaactttt aaaagactgc
    15721 aaaaactgga acaattctgc tcgatcctcg aattcagggc gactagtcgg cagaggcatt
    15781 tgccgataca atacataaac ttcttcaata cgtgttaaaa tttgtctgcc atcatttgac
    15841 tctgaaaatg tttctgcagt ataaatcaat aaaccataga tatgttttcc ataataagct
    15901 ggttgctgga ttctctctag attttccagc atgtctttaa tcacattggc ctgtgctctt
    15961 ctcatagaga agtaagtttc gtagtatagc ggctgtctga gccagtagtt ttcttgatgt
    16021 tctctggcat cttcttggct ttcgcgaatg aatgtcagta aatgttcgca tttgatctgc
    16081 acatcttctt tattttctga taaaagaaat tctgccattt gtctcaaaat atttcgaaat
    16141 tctttttcga tctgtatttg gttttccttc agctgcttcg tacgatctgg catgtaaaca
    16201 tttgcaagca aagcaaatac taatccaatc gccattaatc ccgcttcatt gccgatcagc
    16261 tgccaagaca tgttttcctc cactaaataa tgagtgacca aaaccgaatt tactactatc
    16321 ccttcggtta atttgaagcg agaggcaagc gggatgaaca ggaataaaaa aagactgaag
    16381 ccaaaaatag tatacccaac agcaaaaaaa ctgaacagtg ctgttgccgt agctaatata
    16441 aaggctgcga ttcgattcat gcccgtacgc aatgtagagc gcttggtatt tcctacactt
    16501 aataatgcaa tgatccctgc agctggccaa tacaacagcg ataagctacc cgctgtgacc
    16561 atcgaaaaga agacactgat cactgttttg actgttcgca ttcctatacg catttacttc
    16621 cccctttttt attttaatta tacttgaata atgaaaaact agctatcaga caaaagttta
    16681 ggtatgatag taaaaagacc aaaaaggaga atctctatgc aatttggccg ttttcgacta
    16741 ggtatgcgta cgatcaaaag tgccttagct gtttttttat gtatcttgtt ttttcatttg
    16801 actcaacgag gaatcccgat gattgctgca ttatctgctg ttttttcttt gcgtcaggac
    16861 ttgactacaa ccgtttcttt tggaaagtct aggatcatcg gcaatacatt aggtggtaca
    16921 ttagctatca tttatttttt tgtcaaaagc tattttcaca atgatttttt ggtagaattg
    16981 atcttgcttc ctattctagt tattattgtg atcgtcgtgt cagatggtat taacaataat
    17041 tccggtatca tttccgcaat agctacgttg ttgctgattg cgttgagcat ccctcaaggc
    17101 gaatccgctt tgtatgctat ccaacgtgta ctagatactt ttatagggac attcattgca
    17161 atcggtatca atttctttct tcgtcctcct gaaacagaaa agaaggaaga actatcagaa
    17221 gatttggtcg aactccaaaa gaaagaacga ttgctaagaa caaatttaca agagatagaa
    17281 gctaagatcc aaaaacaaaa gaaaaatagc gaggactaat tttcttttgc aataaaataa
    17341 aaaaatggag agaagaactt taaaaagtct tcctccattt ttgattgaac ggtgaagtgt
    17401 actactttat tcttccttca tcgagttttg ttctgcctct tttggcgctt catacatatt
    17461 tacttggcta gtagtcccac gtgtcgacaa attctctacg atttccttca agtcaagtcc
    17521 tgttgtcgct tttagtgttt cttgagtaga agaaagcaaa tttgtggcat aatttgtcac
    17581 tcggttcgct ccactggttt ctcctgaact gcttcctgta tcgacaacag agattttttc
    17641 gatatttcct agcggctgtg ctgcttcgcg catcaattgc ggtagcatat cgataaccat
    17701 actcaagaca gctgcttcgc catattcctt gaaggcattg gcgatctttt gttttgcttc
    17761 ggcttctgct tttccttttg ctagtgctgc ttcggcttct gcttgtccag ctaaacgtgt
    17821 cttattggct tccgcttctg ccagtgcttc cacacggaat cgttctgctt cggcttcagt
    17881 cacttctttg actttttgag cttgtgcttc ttgttcttta gcgtaccgat cggcatctgc
    17941 ttttttcttc acttccgaat catattgctt ttcacgacgt gtgatttctt tttcttccag
    18001 ttcgatttgt ttttgacgtt ctacgatttt aatctgcatt tcctgttcga ttacttgttg
    18061 ttgtgctcgg gcactttcta aattgtaggc ttggtctgct tttgctttag caacatcttg
    18121 ttcttgtttg taggttgcta atttcagttc tttttctttt aatgattctg cgatctctgt
    18181 ttgtcgctgt aattcagctg cttgtgattc tttttctgct tgagcttttt tgatacgcgt
    18241 ttcttttaga gcttccgctt ctgctatatc tgcatctctt ttgacttggg cgattcttgg
    18301 tttccctaaa gagtcaagat aaccgttttt gtctctgacc tctttgatag tgaaagaaac
    18361 gatgatcaac cccatttttg caaggtctac agaagcgact tcttgtacgc tttgactgaa
    18421 cttatcgcga ttttgataga tttcttctac agtcatagaa ccaagaatcg agcgcaagtg
    18481 tccttctaat acttctcttg cttcattttc cagttcttct cttgtcttgc ctaaaaactg
    18541 ttctgctgct gtagcaatct cttctacaga tgaaccgatt ttgatgatcg aagtaccatc
    18601 acacatcacc ggtacgcctt gttccgtata cacttcaggg gtggagacgt ccaatttact
    18661 cgataataga ctgatccgat tcgaacgttg aaaaactggc aagacgaatg cgccacctcc
    18721 acgcacaatt ttgattttgt tattgctttc atcaatgtgc acatttttgc ttcctaaata
    18781 acttccactg atgatcaacg cttcatccgg ttttgctgtc tggtacttcg ctacaaagac
    18841 gatcagcaac atcagtaaaa tcaaagctgc gattgcgatc gcgataaaaa caactggtat
    18901 ttccatttaa cacacgctcc tttaagcttt tatcatgctt tcataaggca tgacataaca
    18961 gattctttcc ttgatctcaa tgatcaatac tttcgttccc cgctcagctg tctgttcttg
    19021 ggggctgtaa aaacaagctg gacgactaat ggaaccggta acacttttca actgaatttc
    19081 tcccatacct cgtacaggga tcggtgtgat cacaacagct acttgtcctt ccagagtttt
    19141 ttcagaagac gacatcgttg attcggcatg tttcatcggc atcaatatat agaaattaag
    19201 caaaaatacc aacactatcg atatgcctcc actagcaata aataaaaata aagatgattg
    19261 atctgttaaa cgctcaccta gaaaaccaaa taagcttgtg aaagttaacc aagggataat
    19321 aagcatcgga tcaagcgggc catcaaaatc gaatacatct ccaaaaataa tcaataggac
    19381 tgccaatcct gcacaaatca gcaatgtata ccaataaatc gtctcaagtg cgattccccc
    19441 tatcatatac acgcctcctt ccatttctac gtctatcata ccaatgtttg aaaattctgt
    19501 atagcttctt ttaccgactc agtggtgttt tttataggaa ggttgtgttt atcgttgaca
    19561 atttgttaaa tttttgtttt catgttaaaa ttttcacaat aaattctttc gaggtagcct
    19621 atgtttcctt tttttaagaa aaaaccatta aagctccaga acacgatcat ccttgtcgtt
    19681 tgtttctcca cattcgtttc tttgattgtt tctgccatat tgatcaggaa ttttattctt
    19741 tcgatgaacc gatctagtgt gtcagataaa atctctagca ttgctaaaat cgttgccaat
    19801 gataaactca tccaaaaaaa gctgcagact ggtgacccga cacatgcgat tcaaggaata
    19861 acaaatgaaa tcatgattgc atcgaattta gattttatcg ttgtcatgga ccctgaaggc
    19921 attcgatatt ctcacgcaga cccaagttac atcggtcttc ctttctcaag tcatgaagat
    19981 gtgaaaaaag tgattggagg acaagaattt ttctctgaaa aagaaggaat gttagggttc
    20041 ggcttccgct atttcgtccc tatttattat gatcaagaga ttattggtgc agtttgtgtt
    20101 ggcttaacaa acaacacgat tgacaatcaa gtcaaagatg gacaaaaaat gattgttttt
    20161 tcattgatta ttggacttag cgtaggcatc ataggcgccg tttttcttgc ccaaaaaatg
    20221 aaaaaagtac tgcttggact tgaaccacaa gaaatagcag aacagttaag tgaaaaaacg
    20281 attatcgaaa atgaagtaga tgaaggaatc ttagccatct cttctgataa aactatcctc
    20341 ttgatcaata cacctgcaaa aaacattttc taccaaacag agcagctatt ggatttcgaa
    20401 gtaggaaaaa aatcagtgat aatctatatg acgttttgtt taaagagacg attgagcatc
    20461 aaacgaccgt tcggactcaa tccatgtatt taaatgggat agaaactatt tctagcgtta
    20521 cacctctttt tagcaatgac cgttttttag gagcagtcgc aactttacga aatcgatcag
    20581 aagtaatgaa attaatggag gagcttgggg ggacgaaaca atatataaac tccatgagag
    20641 ctcaaaccca tgaattcatg aataaacttc atgtcatttc tggtttgatt gatttgaaga
    20701 agtatgaaga agtcacccat tttatccagc agctaaatca aaattataaa aaagagattg
    20761 gccaagtctc aagtctgatc aaagtacctg caattgccgg ttttttatta ggcaaaataa
    20821 acgaagccaa agaacaatct gttgaaatgc tgttagaaag cgattctttt atccctaaaa
    20881 tggagatgga tgactccatc cattcacttt tgcaagtgtt agggaactta cttgataatg
    20941 caaaagaagc cgtgctacaa aaacatgaaa gaaacggaca ggtccggatt catttaaact
    21001 atgacacaga agctgaagta ttcatcgtga ccgtctgcga tgacggtgtc gggatatcag
    21061 aagaaatctc agaaaaaata tttacgctag gtttttcgac gaagggaatc aatcgtgggt
    21121 ttggattgaa tctcatccat tcaatcgtca aacgccacgg tggattttta gaattttcca
    21181 aacaaacaaa tggcggtaca atcgtctatc tagaattacc aaaaaaggaa gatcaaaatg
    21241 atgacaaatg tcctgataat cgaagatgat ccaatggttg cagaattgaa tcgtactttt
    21301 gtggaaagca tccctaatat gaacgtaatc gaaagtttca gaaatgtaga agaaggaaaa
    21361 aaatatttca gacaaaaaaa cgcagatctg ctgctgcttg atgtatacat gcctggcgaa
    21421 agtggtatcc attttttatc ttggcttaga cagcaaggaa atgatatccc agtgattttg
    21481 atcactgctg cagatgatat cgaaaccata aagaatgtga tccgctacgg tgccttagat
    21541 tatttgatca aaccgttcac tttgcaacgt tttcagctgg cgatcgaaaa gtatcttgct
    21601 ttctctcaga caatccaaac gactgaaaaa gccgatcaga aattgatcga tcaagtattc
    21661 aaccacacca ctcccattca cgaaatacca tcattcatcg atttgcctaa aggtctgtca
    21721 aaactgactt taaaaaaaat aagtgcattt attctaaaag aagatactat tttttcaaca
    21781 aaagaactgg ctcaaaaaag ctcactgtca cgtatatccg tcaagaaata tttgaatttt
    21841 ttagtagaac aagatatatt gaacgaaaga atcaaatatt tggaaatcgg ccggccgcta
    21901 acctgttatg aattgacaaa gaatgggcgg aaaaagctca cagattactg ttgagtcatc
    21961 gaattatccc atacaaaata gtccaagtat acagaagtaa caactgttta cttggactat
    22021 ttttaaacat tttattgacg aatatccatg ttttcttatc ttaattgacg ggctttagga
    22081 agagatacct catttttcgt ttttccagtt tctattattt cacgaaagtt ctcgaaactt
    22141 gtcgaaatca tatttgataa tgcctcatct gtatttgaac ctacatgcgg ggttacaaga
    22201 actctagggt acatttctac taattgctgg atagcagggt ctggaatcgt ctcccatggc
    22261 ttgaaggatc ggaaaaaaac ctctgtttca tttgcaaaaa catcggttgc aaaaccagct
    22321 aaatgattgc ttttcaaagc tcttaaaata gcttcattat cttgaagctc tccacgggct
    22381 gtattaatca agatgctacc ttttttcatt tgagctaaaa atccttcatt gatcatttga
    22441 tcgttttcac ctgggatatg aggtacatga atactgacaa tatcgctttt ggctaataac
    22501 tcttcaagcg agtggaacgt caatactttt ttagcagctt ctgtctgatg aatatcgtag
    22561 ccgatcactt tggctcctag tcccttaaac aaaacagctt ccgtcaaccc tatcctgcct
    22621 gttcctatga tccctactgt gcaatttctg atttcacgac taaacatttg ctcatccact
    22681 cgaaaatctt tgaaagatgt tcggatcgtc gtatacgcag tatttctcaa aagcatcatg
    22741 gcaaaagtca aagataactc tgctatcgca tttggcgaat acgatggtac acgggctact
    22801 gtcataccaa aatcagctgc cgcttgtaga tcgatatgat tgactcccac agtccgtgta
    22861 aaaacataac gaatcccata ttcctgcatt tttgcaagat tggtctcatc tgctatacaa
    22921 tttcccctca acaatacagc atcatggtcc tttgctgaca cgatattgtc atgtgtcaac
    22981 agttcttcat aaagactgag atcataatgg taaatattca atttttcaaa aaaatctatc
    23041 tcgttcggac gaacaccgta acatgcgatt ctcatacttt cttcctccat ttcttatgaa
    23101 aataaaccta tgccatgaat catttgtaat acaacgatcc atacaggtgt cagtaccaca
    23161 gcaaaaatag ttgacagtaa ggatgcattg gaggctaaaa gtgcctccct atcaaaactg
    23221 atagcatagg ctgctgccac tgttgcagct ggtgtagcca tcatgataac gattgttgcc
    23281 agtgcttcaa aattgactgg tagaatattc gttgccgtca aaactgctag aatcacgata
    23341 ttcaatgcag gtacaaatac cactttgact agactgtaat accatgaagt tttttcagat
    23401 gcagcttttt tgaagcttac ttcacctaat gttgcgccga ttgctagcca cgccaatggt
    23461 gaacttaaac cagctaaata atccatcggc tgaaatagcc acggtgcagt ctggtcaatt
    23521 cgcaaaaacg ctacttggct tatctctcct tctgcacctg taactgacac ttgtggcaga
    23581 tacccttgga acagccaaat gaaaaggccg gcaaaagtag cgataatgat tggattgacg
    23641 aacatcgctt tgatattttt agcttccatc ttcaagccac tcatttttat gtatccataa
    23701 gaatacaaaa agattcggta gccaatattg aaaattgagg aatacatcac gccgacagga
    23761 ccataaatcg cactgacgat aggcgttccg aaaaaggtcg tagaaccaaa gatcgtcagg
    23821 acacgcaacg ttgtttgctt atccccttta tagcttaaga aaaaaggttt tgaaactaga
    23881 ataagaataa tataaataac taatccccaa atcaagacat tgattccttg catcagtaat
    23941 tctgtattga tatcttgcat gaaagctcga aatgccagtg ctggcaaagc cactgttaag
    24001 acgactttcg atagaatttt tccaacagat tcagaaaaaa tccccttttt tctacaaaca
    24061 aaccctaata atataataaa aactgttgaa gtgattgcac tgacaatcga catatctgtc
    24121 aaagtcgatc gaaaaatgtc aaataagttc atctttctcc tcctaattat gttcatttat
    24181 tcacaataaa aaaagctttt attttgctaa tacgtattaa
//
LOCUS       DAZKKW010000040        23155 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.040, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000040 DAZKKW010000000
VERSION     DAZKKW010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23155
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.040"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            135..2606
                     /locus_tag="ACG8PO_001512"
     CDS             135..2606
                     /locus_tag="ACG8PO_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFQ0206830.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGYQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKMIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(2709..4445)
                     /locus_tag="ACG8PO_001513"
     CDS             complement(2709..4445)
                     /locus_tag="ACG8PO_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFQ0206831.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSVFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(4442..5146)
                     /locus_tag="ACG8PO_001514"
     CDS             complement(4442..5146)
                     /locus_tag="ACG8PO_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFQ0206832.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            5277..5780
                     /locus_tag="ACG8PO_001515"
     CDS             5277..5780
                     /locus_tag="ACG8PO_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HFQ0206833.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            5740..6078
                     /locus_tag="ACG8PO_001516"
     CDS             5740..6078
                     /locus_tag="ACG8PO_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HFQ0206834.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            6099..7517
                     /gene="ffh"
                     /locus_tag="ACG8PO_001517"
     CDS             6099..7517
                     /gene="ffh"
                     /locus_tag="ACG8PO_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFQ0206835.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            7629..8207
                     /locus_tag="ACG8PO_001518"
     CDS             7629..8207
                     /locus_tag="ACG8PO_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFQ0206836.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            8211..8732
                     /locus_tag="ACG8PO_001519"
     CDS             8211..8732
                     /locus_tag="ACG8PO_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HFQ0206837.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(8811..9365)
                     /locus_tag="ACG8PO_001520"
     CDS             complement(8811..9365)
                     /locus_tag="ACG8PO_001520"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFQ0206838.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            9618..9893
                     /gene="rpsP"
                     /locus_tag="ACG8PO_001521"
     CDS             9618..9893
                     /gene="rpsP"
                     /locus_tag="ACG8PO_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFQ0206839.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            9905..10156
                     /locus_tag="ACG8PO_001522"
     CDS             9905..10156
                     /locus_tag="ACG8PO_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HFQ0206840.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            10281..11072
                     /locus_tag="ACG8PO_001523"
     CDS             10281..11072
                     /locus_tag="ACG8PO_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0206841.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            11242..11763
                     /gene="rimM"
                     /locus_tag="ACG8PO_001524"
     CDS             11242..11763
                     /gene="rimM"
                     /locus_tag="ACG8PO_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFQ0206842.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            11753..12514
                     /gene="trmD"
                     /locus_tag="ACG8PO_001525"
     CDS             11753..12514
                     /gene="trmD"
                     /locus_tag="ACG8PO_001525"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFQ0206843.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            12650..12997
                     /gene="rplS"
                     /locus_tag="ACG8PO_001526"
     CDS             12650..12997
                     /gene="rplS"
                     /locus_tag="ACG8PO_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFQ0206844.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            13370..14032
                     /locus_tag="ACG8PO_001527"
     CDS             13370..14032
                     /locus_tag="ACG8PO_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0206845.1"
                     /translation="MKQNDGRIIWKNQSELKLILTINEFIEKHGITSSRQYQKKLSEN
                     PNSAPSMWFINKKYGSWENLLISIGRENTGYGKWARMSEQELLEIVEAFIKCEKITSQ
                     RMYEQKSVGKNIPSLSTIKKMLGDIRPLFKEKNDGSRFTDFELLLELKNEIIRLKLQD
                     DLSMTKFRKLVQSPKLPSVDTIMKRTNKNWEELMAEIGFDYRRIKIYKQRNNLSKTKK
                     TK"
     gene            14111..17338
                     /gene="fss3"
                     /locus_tag="ACG8PO_001528"
     CDS             14111..17338
                     /gene="fss3"
                     /locus_tag="ACG8PO_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HFQ0206846.1"
                     /translation="MKKPRFKNWRLLATLSLLCQTIGGSLGPTIAFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGVKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSEKAKLVSVLWKEAGTDVDTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     ESKINQAIADYQKKPSFHNSTVKTVLGQSTTVTDTNGLNLSEFDEVVENIANIDYRVN
                     GNQLVITPNANSKESGELTLKKSANTGTPVAYKMAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKETGNVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGAKVTIT
                     EKSVPAPYTIDTTPITATIKSGETISVTSKNTREKGQIILNKMGVETGTDLWNDKYSL
                     AGNTFAIRKDSPTGEIVQEMTTDEKGHAETPKEIANALELGTYYVTETKASNGFVNTF
                     KPVKVELNYVNQTVALVTSNVKGQNQEITGETTLTKEDKDTGNETQGKAEFKGAEYTL
                     FTAKDGQAVKWSEAFKPELVKGTKASDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYTLDETKYPVSIKKVDDNEKNAVITRDVTAKEQVIRFGFDFFKFAGSAAGTAETGF
                     NDLTFKVSPLEGTSEITGAEDEAITAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKADYTKSEYIFTITEKGQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLGTWKDGGKELTDLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     EATKAAQEKDEKAKPVVIAETTATLANKEKTGTWKILHKLTAEQVLDKTIVLFNYVYE
                     NKKPYEAGDDPVAKDASLNNQAQTVNCTVERHVSIQTKAHLEDGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDNTTKEIWKSGKIDYEVNDKEFTKTVLAEKVDT
                     SKYPEGTSFTFKEINYGKDGNINGKHNEDLKEKTQTLTPKKVPTTPSAPEQPEIPTVP
                     SDSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            17452..17766
                     /locus_tag="ACG8PO_001529"
     CDS             17452..17766
                     /locus_tag="ACG8PO_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFQ0206847.1"
                     /translation="MELKFVVPDMAETFGKIRYAGEGEVLTEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            17779..18153
                     /locus_tag="ACG8PO_001530"
     CDS             17779..18153
                     /locus_tag="ACG8PO_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFQ0206848.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIMDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPDQGNSTNQNEGKK"
     gene            18163..18507
                     /locus_tag="ACG8PO_001531"
     CDS             18163..18507
                     /locus_tag="ACG8PO_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HFQ0206849.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLTKALTYDEKMFLIMHQGLL
                     EVGNDAEEDFDVLNWLGEEESFFMVIEVNESLCREAETVLEEIGVEMPDAIEVFLKQL
                     VETKQLPIAVND"
     gene            18578..19930
                     /locus_tag="ACG8PO_001532"
     CDS             18578..19930
                     /locus_tag="ACG8PO_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFQ0206850.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQELRQFDI
                     SKLSLSTIEIVRSIKDLIYLLGSLVLSGSTMLLYIHFFQDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTKETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDIIANRIGIDEVVAESGALRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFVLDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMTRSKAMKEMPNYKTGENYAFLGLPPNFLIFDEYVVYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVDKKFFMKRIKGRGYVDTGGSVISEFYTPLVPKGYDFLREISDIGGLNVNTEG
                     "
     gene            19990..21132
                     /locus_tag="ACG8PO_001533"
     CDS             19990..21132
                     /locus_tag="ACG8PO_001533"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFQ0206851.1"
                     /translation="MTFLDLFAGIGGFCLGMEQAGHQCIGFCEIDDFARQSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     AAILQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQNRERI
                     FLIGHSRDACTEQVFPIIGSSPTSDQNIRNLLNINPSNRGMGGQVYGSDDVAPTLTGD
                     EGIKIALPVDDGISVAGMLLGNFEQGNRVYEITGTAPTLSTKQGGTKIMLRKNETEYQ
                     EVKPGNSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKEVKTDDL"
     gene            21157..21411
                     /locus_tag="ACG8PO_001534"
     CDS             21157..21411
                     /locus_tag="ACG8PO_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0206852.1"
                     /translation="MIGKIQSDPELEEELYRLNFDIRKNGVKVPDDPYVIDEETDARM
                     ELNQVISELERIADFAKEPDIRQYLFEIKAELEIEGITAE"
     gene            21667..22851
                     /gene="mobT"
                     /locus_tag="ACG8PO_001535"
                     /pseudo
     CDS             21667..22851
                     /gene="mobT"
                     /locus_tag="ACG8PO_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MobT family relaxase"
     gene            22848..22985
                     /locus_tag="ACG8PO_001536"
     CDS             22848..22985
                     /locus_tag="ACG8PO_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HFQ0206853.1"
                     /translation="MKRGIVMEIVKDILLVFGGSMLGVTVMCLMHASAAADRAMEKKG
                     K"
BASE COUNT         7694 a         4117 c         4991 g         6353 t
ORIGIN      
        1 cctttttttt tgctatatga caggaatgaa aggtgtttgc aagcttaatg tgaggaacac
       61 tttccatatc tgttctatac tggataggag aagtttggaa atatccagaa aacgaaatca
      121 atcgaggtag ccacatgaag aataaatgga taaatcgaag taaaaaaaag aaagtaacac
      181 aaagatcatc agataaagtc atccgtcttt ttccatcgta taaagaagga ttaacggcag
      241 atcaagtcaa ggaacgaata gaaaaaggtg cagcaaataa ttcagtcgat cccacattca
      301 aaacaaatca gcaaatcgta ttggaaaaca tatttacgta tttcaatttg atctttctta
      361 tcttagccat tttgctttgt ttagtagaat cctataagaa cttgactttt ttaccagtta
      421 tcatagccaa tacaggaatc gccatttttc aggaaattcg atccaaaaag atcttggacg
      481 aactaaatgt tttaaataca gcaaaagtca atgtcatacg agacgggata gaacaaacaa
      541 tagatataga agaccttgta atagatgaca ttgttatttt agagactgga tatcaaattc
      601 cagcagatgc agaggtcgtg gaagggaaac tgcaagttaa tgaggcttta ttgacaggcg
      661 aagcagatga aataaccaaa gaaataggtg atccattaat gtccggcagt ttcattgttt
      721 ccggaaaagc ttacgcccga ttagacaaag ttggagcaga ttcgtatatt tcccagctga
      781 cagtcaaagc caagacaatg ggagaaggag aacagtcgga gatgattgct tctcttaatc
      841 aattgatcaa atggattggg atcatcatta ttcctatcgg tatcctgctt ttttctcaaa
      901 gttacttttt taatggaaac acgatcaaag aaagtgtcgt tgcaatggaa gcagcattga
      961 tcggtatgat acctgaagga ctgtacttgc tgacgacgat tgcattagca ttaagtgcga
     1021 ctaaactagc aaagcaaaga gttcttttac acaatatgaa aagcattgag acattggcaa
     1081 gggtaaatgt cctttgtgta gataaaacag gaacgatcac agaaaataaa atgtctgttc
     1141 aaaaaatgat cgtctctaaa aaacaagaaa taatcagtca gtcagatcaa ttggaagcat
     1201 gcattgctga ctatgcaaaa gcgatggcag gagacaatgc aacaatggaa gctgttaaag
     1261 catattttac aaaaaccact gatcaatcct atcaccatgt tattcctttt tcttctgtcc
     1321 aaaaattcag cagtgtagcg ttggcagaca aggtgtatgt tttaggagcg cccgaaatgg
     1381 ttttgcgcga tcaattgccg gaatatgcgg aagagtttgt ttctttcgct gaaaaaggct
     1441 atcgcgtgtt ggtctttggt gtgtacgatg ggattcttga agaaccagta ttgacagaag
     1501 cagtcacacc attaggctat attctgattg ctaatcctat cagaaaagaa gcaagagcca
     1561 catttgatta cttcaaaaaa cagcaagtag ccatcaaagt gatttctgga gataatccgc
     1621 tcaccgtatc caatgtagct gtccaagcag ggatttcaca tgctgatcag tacgttgatg
     1681 catctaaatt atcagaagaa gaatatgaag cagctgtcga aaaatataca gtttttggac
     1741 gcgtcaagcc agaacaaaag aaaatcttcg ttcaattgat aaagcgtaac aatacagtag
     1801 ctatgacagg tgatggcgtg aatgatattc tagccatgaa agaagcagac tgcagtatag
     1861 cgatggcttc cggaaatgaa gccgccatgc aggcctcaca agtggtctta cttgattctg
     1921 acttttcacg catgccagaa gttgttgcag aaggacggcg cgttgtgaac aatattgagc
     1981 gatcagccag cttgttttta gtgaagaata ttttctcctt tttgctctca ctgttttcag
     2041 tgatctttgc tctgacctat cctttagaac catcacaaat cacattgatt agtttgttta
     2101 ctatcggact tccatctttc ttgcttgctc tagaagaaaa caaaaagcga atcagaggga
     2161 aattcatcat gaatgtgatg gaaaaagctg tgccaggtgg actaacagat atgatcgtag
     2221 tcggtgcgtt ggtgatctgc ggggtgactt tgaatctaaa taaaacagat gtttctacag
     2281 catctaccat gcttttgatt gcagtagggt tcctcgttct ctataaaatc tgttcaccgc
     2341 tgaataaatt cagaagtcag atcattcttt tctgtgctag cggtaacttt ttttcggtca
     2401 tctttttgca taagctgttt tcaatcaccg gtatctcagc tgtatcactt ttgctgttga
     2461 gcattctatt tttctcggca gattcgattt ttaggcattt gaccacattg gtagaatatt
     2521 tgtttgaacg taaaaatgaa aaagagcaaa agccgtttag tcgcgtagct aaaaaatttc
     2581 tatcgatatt taaacgagaa aattgacgaa aagtatctca aaaaagagta aaaaagaaca
     2641 accactcgtg tatctttcga agcaggatga gtcatccaac tttaaaacgc acgagcagtt
     2701 gctctttttt acattttcgg aatggtaacg gtgaaagtcg aacctgttcc aagatgactg
     2761 tctacagtaa tcgtaccatt aaggagttgc acgtagtctt ttacaatcgc taaccctaat
     2821 ccggtcccgc ctgaatgtcg gcttcgcgcc ttgtctacgc ggtaaaaacg ctcaaagatc
     2881 cgttcctgat cctcaatgtc gatacctata ccaaagtctt ggacagaaaa cgatagatca
     2941 tctgttgcct gatagcggat aataatctcg ctgtcgcttt ttgaatattg gacagcattt
     3001 tcaatcagat ttttgataat tggataaagg atatctgttt tagtagtaaa tggttcgttc
     3061 tttccaatca atcgaatcgt caaccgtttc ttttcgatga ttggctgata attttgaacg
     3121 atttgctgga aataaggttc tgtataaatt gtctggatct cgtaagaatt ctcgccatct
     3181 tttgacagct ggatgatttc ccgaatcagt ttatctaacc ggatcgcatc tttttgcatg
     3241 atctcaagaa aagaagtcag agtttgcggg tcatcttttg caccatccag taaagtctcg
     3301 gtaaaaccaa tcagtgaagt cacaggagtt tttaattcgt gagagacgtt gccaacgaaa
     3361 tccttttgta gcttttctaa acgtcgcact ttcgttaagt catacgccac ccctagaatc
     3421 tgtccggtac cttctgaatt agtaaagtag cgcaaactca agtctaatgt ctgccgacct
     3481 tctgtaaggg tgatctcctg atggatcagc ggatgctcag gagtcacttg atgaatcagc
     3541 tgaatgagtt ttggctcttg aatgacttct gtgtaccgct gttcaggttg atagcttacc
     3601 acgtttaaat gtagctgcat ctttggatta agcagtagta aacgagaatc agcatcatca
     3661 atcaggaata cgccaatcat cagttcgttc agtaacgtat ataattgctc ttctgtggta
     3721 gtacacgccc gatacatctt actcatttgt tcgctcaacg cattaatcgt ctgatacaat
     3781 tcttcccatt gatcgggtgt ttgcatgatt acttccgcct gattaggatt ttgcaccatt
     3841 ttttttagta cgggcagcac ggttttcaat ggctggtttt tttgatagat cagataatag
     3901 atcattcctg caagaacaag gaagaaagac agaaagaaga aaaagaccca acggcgaaaa
     3961 ctctcagttc tcggtaaaaa aacgcttgtc ggttctgcga tacgtaaaat ggcttctaac
     4021 tggccacttt ttttgactgg taatgcgaca tataaaagat cctcattcaa agtagtactt
     4081 ttgcgcaaag cgctacctag gttcccacca ttcaatacgg ctttgatttc gggacgggag
     4141 tttcgctgtt cattcaatgt ttcattgttt gtgtcaaata agatctttcc agtagcatcc
     4201 attaaagtaa tccgttcgtt cgactgatgg acgaattctt ccaaagcagt tttgttttgc
     4261 gctgcttgga ggttttcgac atctaattgc cggatcagca gttgtgcttt ttttgtcagg
     4321 tagctttctt gctgttccac gatctgctgt tgaaaataat gggaaatcaa ttgccagcca
     4381 ccaaaaaaca aactaactaa aagaataaga aagaagagac gttcataaat ttttttccat
     4441 ttcatcgttt gggttcctga aacttatagc caaatcctct aatagtcacc aagtactttg
     4501 ggtgttttgg atctcgctcg attttttctc gcaaatgact aacgtgtaca tctacgattc
     4561 ggctttgtcc tgcgaagtca aaattccaaa tgcggtctag caatgtgtct cggtcgatca
     4621 cacggtcttt tcgtttcatg aaataaacaa gtaattcaaa ttctttcggt gttagttcaa
     4681 tcagatggtc gtctacggtc acttgataat tggtcaaatc agcagtgatt tgtccaatcg
     4741 atagatattc aggctcagct tcatcttttg attgcttacg aggttcaatt cgtctgaata
     4801 ttgctttcat acgggctaat acttcgcgag gactaaatgg tttggtcaaa tagtcgtcag
     4861 cacctatttc taggccaatg atccgatcca cttggtcatc tttagccgtc agcatcaaaa
     4921 tcggtgtttc atttttttct tgacgcagcc gctgagtgat ctccatgcca tccatcgttg
     4981 gaagcatcac atctaaaatg atgaaatcaa atgattgttc taatgctaat tctaatcctt
     5041 cgcctccatc tgtggcactg gttaccttgt agccttcttt ttccaaattg aacgtcaaga
     5101 gcgttacaat agaaggttca tcatcaacaa ctagtatttt tttcattcaa atctccttta
     5161 aataggtttc attctgttta tattttagca tagaatcagt acaaagggct attttacaaa
     5221 agaaataaat ggtataatag gaaggcactt tgctgaatag aaaggaagag cagtcaatga
     5281 ttggaatcag tgcatgttta ggcggtctgg cctgtcgcta tgatggacag aaaaaagaag
     5341 taccagaatt gatggaacta gttgaaactg gagaagctgt catggtctgt ccagaagtga
     5401 tcggcggatt accaattcct agagatcccg ctgaaatcat tggtggaaat ggattcgatg
     5461 tgtggaaaga tcaagcaaaa gtatggacga caagcggcga agatgtaacg gaagcgtaca
     5521 aaaatggcgc gatcaaagcc tatcaaaaat tgcaggaaaa acacattgat ctagtcatca
     5581 tgaaggaaaa aagcccttca tgcggaaccc attcgattta tgacggaagt ttttccggaa
     5641 taaaaaaaaa tggtgccgga gtggctactg cttattttat tcagcaaaaa atgactgtgc
     5701 tctctgatga agaatggctg aaaactagag gtgagcagaa tggaaatcga aaagaccaat
     5761 cgaatgaacg cgctctttga attttattcg accttattga cagaaaaaca aatgaactac
     5821 atggaattat attatgcaga tgatttttca ttaggtgaga tcgcggaaga atacgaagta
     5881 agccgacaag cagtatatga caatatcaag cggacaagca agatcttgga ggattatgaa
     5941 aaaaaactcc atcttttttc taattatatt gttcgagaac aggtattgga aaatatgact
     6001 gcatatatta cagataagta cccggaagat aaaaaactac ttgaatatgt acagcaaatt
     6061 caagcaatcg aagaataaat aaaaggaatg aagaaaatat ggcatttgaa agcttaactg
     6121 aacgtctcca gcaggcgatg ggaaaaataa gaaaaaaagg aaaagtatca gaagctgacg
     6181 taaaagaaat gatgcgggaa atccgccttg ccttactaga agccgatgtt aacttgcaag
     6241 tagtcaaaga ttttactaag cgcgtacgcg aacgtgcggt aggggcagaa gtgctggaaa
     6301 gtttatcacc tgcacaacaa atcgtaaaaa tcgttgatga agaattgacg atcacacttg
     6361 gttctgaaac agcagagtta aataaatcac caaaaatacc aactgttatc atgatggctg
     6421 gtcttcaagg ggcaggtaaa acgaccttta ctggtaaatt agccaattat ttaaagaaaa
     6481 atgaaaatgc tcgtccgctc ctaatcgcag gggacgtata tcgaccagct gcgatcgacc
     6541 aattgaaagt attaggtcaa cagctagacg ttcctgtatt tgatatggga actgaagtca
     6601 gcccggtaga gatcgtccgt caagggatgg agttagcaaa agaaaagaaa aacgactatg
     6661 tattgatcga tacggcagga cgtctgcata tcgatgaaac attgatggat gagttgaaac
     6721 aaatcaaaga attgacgcaa ccaaatgaga ttttgcttgt tgtcgatgcc atgactggtc
     6781 aagatgccgt caacgtagct gatagcttca accagcaatt aggtattaca ggggtcgtca
     6841 tcactaaact tgatggggat actcgaggcg gggcagcact atcgatccgc tcagtgacag
     6901 gcgcaccaat caaattcatc ggttctggtg aaaaattgac tgacttagaa gtattccacc
     6961 cagatcgtat ggctagccgt atccttggta tgggggatat gctgacattg attgaaaaag
     7021 cgcagcaaga ctacgacgag aaaaaagcgg aagaacttgc acagaaaatg cgggaaaatt
     7081 cattcgattt caatgacttt attgaacagc ttgatcaagt gatgggtatg ggcccactgg
     7141 aagatctaat caaaatgatc ccaggaatga atcaagtccc tggaatcgaa aacgtcaagg
     7201 tagatccaaa agatgttgaa cgcaaaaaag cgatggttta ttctatgacg ccagcagaaa
     7261 gagcaaatcc tgatctgttg aacccaagcc gtcgtcgccg aattgctgcc ggttctggaa
     7321 acagtgttgt tgaagtgaat cgaatgatca aacaattcaa ggaatcaaga aaaatgatgc
     7381 agcaaatgtc caaaggggat atgaacatcc caggtatgga ccaaatgttt ggttcaggcg
     7441 tcaaaggaaa actaggcaaa atggcgatga atcgaatgat caagaaaaat aagaagaaaa
     7501 agaaaaaacg gaaataagat gaaaaaaaca gcttcggtac agactgaaaa tgtctactgc
     7561 cggagctttt ctatcagcaa tttttattct ttactaatac aattactcta aacgaagagg
     7621 tggcacagat gagatgtccg tggagtgaac aaacagaaag tatgcggcaa tatcatgata
     7681 ctgtctgggg tgttcctgaa tatgatgacc aaaagctgtt ccgaaaattg atgcttgata
     7741 ttaatcaagc cggattgagc tggcagacga ttttaaataa aagtgaagca ttcgatgaag
     7801 catatgatcg attcgatatc aaaaaagtcg caaattatgg agaaacaaaa atcagtgaac
     7861 taatggaaaa caaaggaatt attcgtaacc gaagaaaaat cgaagcagcc gtccaaaacg
     7921 ccaaagcagt cttgaaaatt caagaagaaa tcggcagttt ttcagagtat ctttggtcgt
     7981 tttctaatgg aaaaatcgtt gattcttctt atcattcaca agaagaagtc ccaacaacaa
     8041 gtcctctttc agatgaatta gcaaaagatc taaagaaaag aggatttaaa tttatcggaa
     8101 gcacaacaat ctatgctttt ttagaagctg tcgggatcat caatgatcac cttgacagtt
     8161 gtttccgcaa agaagaggtc gtacgtatcg gcagaaaggg cgaataaagc atgcgaaaaa
     8221 tcatatttta tggtgctata agtttagatg gctatttggc aacgaaaacg gatgatcttc
     8281 aatggctttt tgacactcca acaggtaagc aaactactta tgaagctttt tatgaaacaa
     8341 tcgatacgac catcatgggc agaaaaactt atcaggaagc aaaaaaatat ttggagaccg
     8401 aagcaattta tccagagaaa aagaatttcg ttttttccac ccacgcacaa ttacaattgc
     8461 cagatgctga ggtcgtaaac gaagatcctg ttgcttttct aaaaaatcta caaatgacag
     8521 aaggaaaaga tatctgggtt gtgggtggcg gtcgattgat caaaccgcta ttggaacatc
     8581 aaatgattga tgaatggtat atccagatta cccctgtatt attaggggac gggattcctt
     8641 tattccaaaa aggaacatat gaagcgcgtt ttgaaatgct agacacgaca cgattcggtg
     8701 aatttatcga attgcattat gtacgaaagt agaagtaaat aaaataaaaa aggcaactat
     8761 atgcagaaat gtcctgtaca tagttgcctt ttttattacc gttatgcttt ttactctgtt
     8821 aatttgttag aaaaatctag atacgcatct aaaatcgttt ggaagaaact taatgttcct
     8881 tcaacgatat taccattttc atcaattaga ttcgcaacgt ttccgatata tgcttccggt
     8941 tgctgcaagg ttggcacatt caaaaagaca agtgattggc gtagatggtg gtttgcgcca
     9001 aagccgccga tcccacctgg tgatgcagta acgactaaac ctggtttatg atcccaaaca
     9061 ctgtgtccat aaggtcttga gcctacatct aaagcatttt tcaatactgc tggcacagaa
     9121 cggttgtatt caggagtaac gaaaacgaca ccgtctagtc ccctcacttc ctcacgaaaa
     9181 cgagtccatt cagcaggagc ctgttctgga gtttcgagat cctcattgta aaatggcaga
     9241 tcatcgattt ttacaaatac actttcgtac ccttcaggaa gcaatttcgc aaaagcttct
     9301 gctacttttt tgttgtaaga atcttttctt aaactaccaa taaaaaatcc aatttttttt
     9361 gtcatacgat ttttcctcct tcaaactatc tataataatc ttacaaaaga taagtaactg
     9421 gagcaaataa aaacacttac tgacaaaagc tacatagaag atagattact tatttttaac
     9481 aaaaaagaag tctaaaatct tctattttct aaaaaaaatc gaatttaatc aagctgtaaa
     9541 gaaaaaaagc tttacagggt aaaaaaaaac tgttacacta catcttgtga ataaaacaat
     9601 ggaggtggaa ataaataatg gcagttaaaa tccgtttaaa acgtatgggt tctaaaaaga
     9661 gtccgtttta ccgtatcgtc gtagctgatt cacgttctcc tcgtgatgga cgtttcatcg
     9721 aaactgtagg tacttacaat cctttgaaag accctgcaga agtagtttta aaagaagact
     9781 tagttcttga ctggttatca aaaggtgcac aaccttctga cactgtacgt aacatccttt
     9841 caaaagaagg cgttatgcaa aaacaccacg aagctaaatt ctcaaagaaa taaggtgacc
     9901 ggatatgaaa gatttaagcg acttagtctt aacaatcgtt cgtccgctag tcacttatcc
     9961 tgatcaagtc aagttagacg tcgtagagtc gaaagacttt tacgagtaca atctaacagt
    10021 ggctcctgaa gatattggcc gtatcattgg aaaacaaggg cgtgttgcta aagcaatccg
    10081 cacaatcgtt tacggtgttc ggatcaatgc accgaaaaaa gtgcgtttga atatcatcga
    10141 tgataaggaa aagtaaataa acgtgctgta ctcgaatttt cgagtgcagc ttttttgttt
    10201 tttttattaa ttgaagaaat gattatttgg caaaacaagc aagaaagcgt atccttatag
    10261 gaggagggag tgaacagcca atgagaaaga aaatgttatt tattccattt gctctattgt
    10321 taagcgcttg tagttcggtt actcaagaat cagacaatac acatctatca agcagtatat
    10381 ctgaaactgt taccagtcca gaaaaaagta gtaccaaaac ggcttccacc actgaacaaa
    10441 caatggctac ctcaaataat gagaaaaaga ctgctttaga ccagttgaaa gaacaacagc
    10501 caaatgttcc catgcctcta gatgtaccag tctcttcagg ctatctcaat atcgcagcga
    10561 cacatacaaa gcaaggttat tctattttat attacagaac agatcgtccc ctcgggttga
    10621 atgctgacga attgaatcaa gaaacgccta ttgcaaccta tctatatcaa tatgggtttg
    10681 cttctagtca agaaacaatc caagttttgc agccatttga gatcgacaca aatggtcagc
    10741 aagtggactt aggttctcga atcacagggt atcagcaagg tgcagcaggt tcaagttttc
    10801 ttgaatggca agaaggaaac tggcgtattc gaatccgtgg gaataatata gaaggccaag
    10861 atcccttact attagctaaa gaaatcgtag cttatttgga agaaaacagt ctaccagcac
    10921 ctgaacaatt tggtaaaatt actgttgata tgggtgatac gacgaatcga gccgtagaag
    10981 taagctggca agaaccgaaa aatgcctata cgatcactca tcaagatcct atgtctgcgt
    11041 tgaagatggc agtttctatg aagaggttgt gaaatcagcg cgctgatctg agcccatcat
    11101 ttaactccaa gtcacaggaa gtaatcaaaa atagcacgcc gcatttttgc ataattttgg
    11161 cttaatacct taaaagaaaa ctagaattgc ctcttcacac atgctaaaat aaaacagaca
    11221 aaaaagaaat ggggatataa gttgactgaa tacttgaatg taggaaaaat cgtaaacaca
    11281 caaggtatca aaggcgaagt ccgtgttatc tcaacaacgg atttcccaga agaacgttac
    11341 aaaaaaggaa cggtgctaac actattccaa gacggaaaag cacctgtgga attaacagtc
    11401 aaaagtcatc gcaaacataa aaattttgac ttattgagtt ttgaaggaca tccttctatc
    11461 aatgatgtgg aaaaatatcg ggatggcata ttaaaagtgt caaaagacaa ctctgtagat
    11521 ctagctgaaa atgaattcta ttaccatgag atcatcggtc tgcatgtatt agaagatgga
    11581 actgagttag gtaaagtaaa agaaatcttg tcaccaggtg ccaatgatgt ttgggtcgtt
    11641 caacgtccga aaaaagctga tatattgctt ccatatatcg cttctgtcgt caaagaagtc
    11701 gatttagaag ctggagtggt ccgtgtagaa attccagaag gactgattga cgatgaagat
    11761 tgatgtattg actttatttc caagaatgtt tgaaggtcct atgggggaat ccatcatcgg
    11821 taaggctgta gataaaggat tattagatat caatatctct aacttccgcg attattcaga
    11881 taacaaacat cagacagtag atgattatcc atacggcggt ggagcaggta tgctgctcaa
    11941 agtccagccc atctatgata acatccaagc cattgaaaaa gctgcaccgg atgtcaaaaa
    12001 aagagtaatt cttcttgacc cagctggtaa acgattcgat cagaaaatgg cagaagaatt
    12061 ttcacaagaa gaacacttag tattcatttg tggtcattat gagggatatg atgaacgtat
    12121 ccgcacatta gtgacagatg aagtttcctt aggagattat gtccttactg ggggagaatt
    12181 aggtgcgatg gtcatgatcg atgcaacagt tcggcttttg ccagatgtgt tagggaatca
    12241 gacttcagct caaacggatt cttattcaac cggtttattg gaacatcccc aatatactcg
    12301 cccggcagaa tataaaggga tgaaagtacc agaagtattg acaaatggaa atcataaatt
    12361 gattgaagaa tggcaattaa aagaatcact aagaaggacc tatcaacgtc ggcctgatct
    12421 tttggaaaca cttgaatgga cacctcaaat gacgaaattt ttagaagaaa tcaagaaaga
    12481 agaacaagaa aaaacagcag atgaagccaa gtgacttttt ctaaaatctt ctttacagta
    12541 ggttgataat atgttagtat tttatggtga gtgcaaaagc acttaactat tacgatattc
    12601 cgctgtgaga aaagctcata agaatatttg gaataaggag aagaaaacca tgaatccatt
    12661 aatcgaagaa ttaacaaaag aacaattacg ttctgacatc ccagcgttcc gccctggtga
    12721 cactgtgcgt gttcacgcaa aagttgttga aggtactcgt gaacgtatcc agttatttga
    12781 aggtgttgtt atcaaacgcc gcggtgctgg aatcagcgaa acttatacag tacgtaaagt
    12841 ttctaacgga gtgggcgttg aacgtacatt cccattgcat acaccacgtg ttgcaaaaat
    12901 cgaagtagtt cgctacggta aagtacgtcg tgcaaaattg tactacctac gtgcattaca
    12961 cggaaaagct gctcgtatca aagaaatcag aagataaaca cggaaaactt ccttggaaat
    13021 ctgaaatttg tgaaaagtcc ttgtatatca agggcttttt cttttttctc tatcacagtt
    13081 ttaagccatt tttggctgtt ttggaccatt tttcaatcat attcccttaa aaattgtcct
    13141 agattgtccg acattgacta tcagtgtcgt caaaataggg caaaatttag tagtcagctt
    13201 gacgacaaat ataatacgaa aaataccaaa acatttaccg aatttagcaa cttttgaacc
    13261 caagtttctc aaagggtcag ctattagcaa aaaagacaaa acatgtactt ttctactaaa
    13321 aagttgatgt gataggcttc tatctaaata actattagga gtttttatag tgaagcaaaa
    13381 tgatggacgt atcatttgga aaaatcaaag tgagttaaaa ctgattttaa caattaatga
    13441 attcatcgaa aaacacggaa ttacatctag ccgtcaatac cagaaaaagc tatcagaaaa
    13501 tccaaattct gcaccaagta tgtggttcat taacaaaaaa tatgggtcat gggagaattt
    13561 attgattagt ataggacgag aaaatactgg gtatggaaaa tgggctagaa tgtcagaaca
    13621 agaattattg gaaatagtag aagcctttat taagtgcgag aagataacct ctcaacgaat
    13681 gtacgaacaa aaatcagtag ggaagaatat tccttcactt agtaccatta aaaagatgtt
    13741 aggagatata cgtccacttt ttaaagaaaa aaatgatgga tctcgattta ctgatttcga
    13801 actgctgtta gaactaaaaa atgaaataat ccgattaaaa ctacaggatg atttatcaat
    13861 gacaaagttt cgaaaactag ttcaatcccc aaaactacca tcagttgata caattatgaa
    13921 aagaacgaac aaaaattggg aggaattgat ggcagagatt ggatttgatt atcgaagaat
    13981 caaaatttat aagcagagaa acaacctatc caagacaaag aaaactaaat agtatcactc
    14041 taaatcagac gttcaaaatt aaaatttgga cgtctttttt attacccaaa aacgaaagga
    14101 gatttatttt atgaaaaagc caagatttaa gaattggcgt ttgctcgcaa cactgagtct
    14161 attatgccaa acaattggtg gatcgcttgg cccaacgatt gcctttgctg atgaaattac
    14221 tcatccacaa acggtaactg tggaattgga tttggctcac cagtatgctg tcgaaggtac
    14281 atttagcgat ggtcgtccca tgtcagaagt taccgttccg cactatgcag tttacaatgg
    14341 tgtgaagcaa gatattttct gtattgagcc aggggtgccg atttacaatg agttcactcc
    14401 tggttatgag aaaaatccat tgcctgatat gtcggagaaa gcgaaattag tttccgtttt
    14461 atggaaggaa gctggtacgg atgtggatac acatattgtg gcccagaaga tgatttggca
    14521 agaagttaat ggctacacgc ttcactcaat taaacgttct gatggtagtg cagtaaatat
    14581 cgcagccatt gagtcaaaaa tcaatcaagc cattgcggat tatcaaaaga aaccaagctt
    14641 ccataactcc acagtgaaaa ccgtgttagg tcaatcgact actgtgacag atacgaacgg
    14701 cttgaattta tcggagtttg atgaagttgt cgagaatatc gcaaatattg attatcgtgt
    14761 gaacggcaat caactagtga ttactccaaa tgccaattca aaagagagtg gtgaattaac
    14821 cctaaagaag tcagctaata caggaacacc ggtagcttat aaaatggctg gtcaacaaac
    14881 tttgatggcg ggggcaattg ataagcccaa tacctacaca atcaaaattg atgtggaaac
    14941 tgagggttct ttgaagatta aaaaagtcga taaagaaaca ggaaatgttg taccgggaac
    15001 cgttttccat ttagactttg gaaaaacatt acctgcaaaa gacgtgacca ccgataaaga
    15061 aggcattgcg acattggatg agattcctca tggcgcgaaa gtaaccatta ctgaaaaatc
    15121 agtgccagct ccttatacga ttgacactac accaatcacc gcaaccatta aatctggtga
    15181 gactatttct gtgacttcaa aaaatactcg cgaaaaaggt caaatcattt tgaataaaat
    15241 gggggtggaa acaggaactg atctttggaa cgacaaatac tcattagccg gaaatacgtt
    15301 tgccattcgc aaagacagtc caaccggtga aattgtccaa gaaatgacca cggatgaaaa
    15361 aggtcatgcg gaaacaccaa aagagattgc caatgcgttg gaattgggaa cctattatgt
    15421 gaccgaaacc aaagctagta atggctttgt gaataccttt aaaccagtaa aagtcgaatt
    15481 gaactatgtc aatcaaaccg tggctcttgt taccagtaac gtaaaagggc aaaaccaaga
    15541 aatcactggt gaaaccactt tgacaaaaga agacaaagat accggtaatg aaactcaagg
    15601 taaagctgag tttaaaggag ctgaatatac tctctttact gcaaaagatg gtcaagcggt
    15661 caaatggagt gaggctttta aaccagagtt agtgaaggga acgaaagctt ctgatgaaac
    15721 ggtgactttg actttagatg aaaagaatca agttgctgtt aaacacttag cgattaatga
    15781 gtattactgg caagaaacca aagcacctga aggatatacc ttggatgaaa caaagtaccc
    15841 tgtttccatc aaaaaggtgg atgataacga aaaaaatgcc gtgattaccc gagatgttac
    15901 ggcaaaagaa caagtcattc gctttggctt tgatttcttt aaatttgctg gatcagctgc
    15961 tgggactgcc gaaactggct tcaatgatct gacctttaaa gtatcgccat tggaaggaac
    16021 cagtgagatt acaggtgccg aagatgaagc aattacagct tataacgagc aactaggttt
    16081 tgatggctat ggcaagtttg aaaatcttcc ttatggggat tatttacttg aagaagtaga
    16141 ggctccagaa ggatttcaaa aaattacgcc attagaaatc cgttctactt ttaaagaaaa
    16201 taaagcggat tacactaaga gtgaatacat ttttacgatt accgaaaaag gacaaaaaca
    16261 accaattaaa acggtgaccg ttccttacga gaaactgacg aataaagcat tttctgttag
    16321 cttgaatcgt ttgatgctct atgatttacc tgaagaagaa gatagcttaa cttctcttgg
    16381 gacttggaaa gacggaggca aagaattgac agaccttgat tctaccgagc tagttgataa
    16441 attgagctat aacttgcatg aaatcaaaga agactggtat gtggtagcgc aagccattga
    16501 tgtggaagcg acaaaagctg cccaagaaaa agatgaaaaa gccaaaccag tggtaattgc
    16561 cgaaacaacc gcaacgttgg cgaataaaga gaaaacagga acttggaaaa ttttgcataa
    16621 attaaccgca gaacaagttt tggataaaac catcgtctta ttcaactatg tgtatgaaaa
    16681 caagaaaccc tatgaagcag gtgatgatcc agtagcgaaa gatgctagct tgaacaatca
    16741 agcccaaact gtcaattgta cggtggaacg ccatgtttcc atccaaacaa aagcccacct
    16801 agaagatggt tcgcaaacct ttactcatgg ggatgtggtg gatatgttcg atgatgtgtc
    16861 gattacccat gatgtactgg atggctcaaa agaagctttt gaaacaattc tgtatgcatt
    16921 acttccggat aatacgacca aagaaatttg gaaatctggc aaaattgatt atgaagtgaa
    16981 tgacaaagaa ttcactaaaa ccgtacttgc ggaaaaagtg gacacaagta aatatccaga
    17041 aggtacaagt tttaccttca aagaaattaa ctatggcaaa gacggaaaca tcaatgggaa
    17101 acacaatgag gatctaaaag aaaaaactca aactttaaca cctaaaaaag ttccgacaac
    17161 accaagtgca ccagaacaac cggaaatacc aactgttcca agtgactctc aagaatctag
    17221 tcctacagta aagacattcc cacaaacagg ggagaaaaat tctaatgttc tgctgtttat
    17281 tgggtttacc ttgatctttg cgactgctgg gtattatttc tggaatcgcc gaaactaagg
    17341 tgatgtgatg aaacaactaa ataggaaaaa ccaacgtgcg cgaccgcctc ctaaaaaaag
    17401 tggtcgcgct atttttgtat ccaaaactaa aaatgaaaag aggaaaagaa gatggaattg
    17461 aaatttgttg tgccagatat ggccgaaacc tttggaaaga taaggtatgc cggcgaaggc
    17521 gaagttttaa ctgaaggata tgggcggaat actacagtaa ttggtcgtag ttatcatttg
    17581 tattccagta agcaacgagc cgatgatatt gaagtggtgg tagctgcgga agccggcgaa
    17641 aaggattttg atcaagatca accactaaaa gccgtgaatc cccatttggt tgccaaaggc
    17701 tatgagattg agaatcgtgg gtttaccgac tacgtgttgt atgtcgatga tttagtgaaa
    17761 gcataggagg aaaagaagat gagattaaca gaaggtattg ttgtagattc aggactaacg
    17821 tttgggaaat tacgattttc cgcattacgc cgagaagtac ggaagcaaaa tgaggatgga
    17881 acggttagta acgaggtaaa agaacggact tataatttga aatcctctgc gcaaggtcga
    17941 atgatccaag ttagtatccc agcaaatgta cccttacgcg agtttgccta tgacgcagag
    18001 gtagagttgg tcaatccaat catggatacg gtcgccaatt atgttttccg agaaggaact
    18061 actgtcaatt ggttcattaa ggccgatgat ttagtcttga aacgacaacc agatcaggga
    18121 aattctacaa accaaaatga aggaaagaaa taggtgaagc gtatggaaat taatgttgaa
    18181 catattttag attgtctcga tcaatatgga aaaggtgagc taacggagga acaactcact
    18241 aaagcattaa cctatgatga aaaaatgttt ctgattatgc atcaagggtt actggaagta
    18301 ggtaacgatg ccgaagaaga ttttgatgta ctgaattggt taggggaaga agagtccttc
    18361 tttatggtta tcgaagtaaa tgaaagtcta tgtcgagaag ccgaaaccgt attggaagaa
    18421 attggtgtgg aaatgccgga tgccattgaa gtttttttaa agcaactagt ggaaacgaaa
    18481 caactcccta tagcagtaaa tgactagcat aactgcttga aattggatca atgaaatcct
    18541 atgaagcttt tttcgattgg aggtggaaac cataaaaatg tacaaagggc atcgtatacg
    18601 ggcaggagat cagcacttgg tttatcactt tgttcttggc tggctactcg ctctgttcat
    18661 cggttggatg agcgtctttt attttcaaga gcttagacaa tttgatattt ctaagctatc
    18721 actttctact atagaaatcg ttcgttctat aaaagattta atctatttgc taggaagtct
    18781 cgttctttca ggaagcacga tgttgcttta tatacacttt tttcaggatc attggcgcag
    18841 tttgtggcat cgacaaaagc tagcccggat gattctagaa aatcattggt acgaagtaaa
    18901 acaaactcag agtgaaggat ttttcaaaga cctcaatagt agtcgaacca aagagacaat
    18961 cagttacttc cccaaaatct attaccgtat gaaggatggt ttactttcta ttcgcgttca
    19021 aatttcactg ggaaaatatc aagatcagct tttaaagctg gaaaagaagt tagaaagtgg
    19081 tctatattgt gaactagtag aaaaggaact caaagactcc tatgtggagt acactttact
    19141 gtatgatata attgccaatc gaattgggat agacgaagta gtggcagaaa gtggggcttt
    19201 gcgattgatg aaaaatcaag tatgggctta tgattcttta cctcatatgt taatcgctgg
    19261 cggcacaggt ggtgggaaga cttacttttt actcactatc atcgaggctt tattaaaatc
    19321 tgatgccgag ctttttgttc ttgatcccaa aaatgcggac ttagccgatt taggtacggt
    19381 aatgcctcat gtctattccc aaaaggagga aatttctgct tgtgtggaag atttctatga
    19441 gcgcatgatg actcgtagca aggcaatgaa agaaatgccc aactacaaaa caggagagaa
    19501 ttatgcgttt cttggacttc caccaaactt tttaatcttt gatgaatacg tggtttatat
    19561 ggaaatgtta acgacgaagg aaagtgcggt gattttgaat aagctcaaac aaatcgtgat
    19621 gttaggtcgt caatctggtt tctttctgat tctagcttgt caaagaccgg atgcgaaata
    19681 tttaggagac gggattcgcg atcaatttaa tttccgggtg gctttgggtc gtatgagtga
    19741 actgggatat tccatgatgt ttggcgaggt tgataaaaaa ttctttatga agcgtatcaa
    19801 aggtcgaggc tatgtagata cgggagggag tgtgattagt gagttttata cgccacttgt
    19861 accaaaagga tatgatttct taagggaaat tagcgacatt ggtggattaa acgttaatac
    19921 ggaaggataa aataatagta tgtagcctag gagtgtcaag aatcctgggc tttttttacg
    19981 aggtgattta tgacattttt agatttattc gctgggattg gtggcttttg cttaggtatg
    20041 gaacaagcgg gtcaccagtg tatcggcttt tgtgaaattg atgattttgc tcgccagagt
    20101 tataaggcaa tccatgatac aagtaaggag gtggaaatgc atgacatcac aagtgtatca
    20161 gacgagttta ttcaatctct cggaccagtg gatatccttt gcggtggatt tccgtgccaa
    20221 gctttttcaa ttgcgggaaa gcggcaagga ttttccgata ctcgaggtac tttattcttt
    20281 gaaatcgctc ggttcgccgc tattctccaa cctaagtttt tattccttga gaacgtccgg
    20341 ggattactca atcacgaagg aggggctacg ttcgagacga tcctccgaac gctggatgga
    20401 ttggggtatg atgtggaatg gcaagtgctt aactcaaagg cctacgttcc ccaaaaccgt
    20461 gagcggatct tccttatcgg acattctcga gacgcatgta ccgaacaagt atttcctatc
    20521 attggatcgt ctccaacatc tgatcaaaac atcagaaact tattaaatat caacccttcc
    20581 aatcgaggaa tggggggaca agtctatggg tcagatgacg tagcgcctac cttgaccggt
    20641 gatgaaggaa tcaaaatcgc attgccggta gatgatggaa tttctgtagc gggtatgtta
    20701 ctgggaaatt ttgaacaagg caatcgggtt tatgagataa ctggaacagc gcctactttg
    20761 tcaacgaaac aaggcggaac gaagatcatg cttcggaaaa acgaaacaga atatcaagag
    20821 gtgaagccag ggaacagtgt gaacctggct tttcctaatt ccacaagtcg tcgaggccgt
    20881 ctgggaaaac aaagtgtgca taccttatta acaggagacc aacaagcagt cgtcacggat
    20941 cagtatcaaa ttcgaaagtt aacacctagg gagtgctggc ggcttcaggg ctttccggac
    21001 tgggcttttg atcgagcttc tcaagtcaat tcagatagtc agttgtataa gcaggctgga
    21061 aattctgtaa cagttccggt catttttgat attgctagaa gattaaagga ggtaaaaaca
    21121 gatgatttat gatgagttga ttggggaatc tactgggtga ttggcaagat tcagtcagat
    21181 ccggaattgg aagaagaact gtaccggctc aattttgata ttcgcaaaaa cggggtgaaa
    21241 gtccctgatg atccgtatgt aattgatgag gaaacggatg ctcgaatgga gctgaatcaa
    21301 gtcatcagtg aattggaacg aattgcggac tttgctaaag aacctgatat ccgccaatac
    21361 ttgtttgaga taaaagcaga gttggaaatt gagggtatta ccgcggagta acggccaaat
    21421 aaaccactga taggcactga ccgcggggga tttgcgaagc agaaatcctc cgtggtgctg
    21481 tgcctgttta tggacggcgt tagccggcca tgatgtctca ccccccgtat ctaacagggg
    21541 ggtacaaaaa cacaaaaaat aagcaagcaa cgataaggaa tggctgtcct atcaaggata
    21601 ataagcaatt tgtgtagatg ttaactgatt tgaaaaagtt aacaccttaa atttggaggt
    21661 gagaacttgg cacaaaggaa tttagattat cggctattaa aagaccggcg taatgaatat
    21721 ggcgtttcac aaaataaatt agctacgact tgtggactta gtcgcccgta cttaaatcag
    21781 atagaaaatg gtggcgtaac cgcatcgaca aaaaccatga ggaaaatttt tgaccaactg
    21841 gaaagtttca atcccgagtt acctttaacg ttactgtttg actatgtaag gattcgcttt
    21901 cccacaacgg atgcgaggaa aatcattcaa gagattctcc atttgaaatt tgattatatg
    21961 ctccacgaag attacgcctt ttactcctat caagaacaat atgttatggg agatattgtg
    22021 gtgatgttgt cacatgaaga agataaaggc gtccttttag aattaaaggg tcgtggttgt
    22081 cggcagtttg aaactttttt actcgcccaa aagcgtagct gatatgactt tttcgaagat
    22141 tgtctaaaag ccggtggcgt gatgaaacgc ttagacctgg caatcaatga tcgagttggc
    22201 ctgttagata ttcctgattt aacgaaaaaa tgtcagaagg aagaatgtat ctccttgttt
    22261 cgtaccttca aaagctatcg ttccggggaa ctgttgaaac ctgatgataa agatggtatg
    22321 ggcaatactt tgtatattgg cagtcttaag agtgaggtct atttctgttt gtacgaaaaa
    22381 gactatgagc aatatatcaa gttagggatt cctttagacc agaccgaaac gaaaaatcgt
    22441 tttgaaatcc gactgaaaaa tgatcgagct tatcatgcaa ttcaagattt gttaaaaggt
    22501 cgcagtattg aaagtaccac attttccatt attaaccggt acttgcgatt tgcggataag
    22561 gtggaaggca aaaggcgaac taactggccc ttgaatgaac aatgggggcg cttcattgga
    22621 cggaatcaga aggaaattca attaacttct gaaccgaagc cttataccat tgaacgaacg
    22681 ttgaactggc ttggacgaca agtcgcccct acgtggaaga tggccaagga gttggatcgg
    22741 ttgaatcaga caacgtacat tcaggatatg gtaaataata ctcgactatc agaccggcac
    22801 aaaaagattt tggagcaaca aagtatggca attgaaaatt tgattatatg aaaagaggaa
    22861 tcgtgatgga aattgtgaaa gatattttat tggtgtttgg tggcagtatg cttggtgtta
    22921 ctgtgatgtg cttgatgcat gcaagtgcag cggcggaccg agcaatggaa aagaaaggaa
    22981 agtgatgaaa tgaatttcgg tcaaaatttg tgtgcgactc gttccttagt gaaaagctaa
    23041 ggcactaata aaagaacgaa aaagtttggt tattaggaga actgataatc tgacaggtgg
    23101 aatgatgaag taacgctctg aaacgcctgc cctgagcctc cgacaagcat tgtat
//
LOCUS       DAZKKW010000041        22315 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.041, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000041 DAZKKW010000000
VERSION     DAZKKW010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22315
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.041"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(102..620)
                     /locus_tag="ACG8PO_001537"
     CDS             complement(102..620)
                     /locus_tag="ACG8PO_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HFQ0206854.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
     gene            complement(622..1239)
                     /locus_tag="ACG8PO_001538"
     CDS             complement(622..1239)
                     /locus_tag="ACG8PO_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HFQ0206855.1"
                     /translation="MMNRSIGKKANDYFLEIIEWIPRLIHLQLLWFLCVLPVFTLGTA
                     SRTVLYMIYHYKKNEEKKLGSLFWKKFRENFSLYGKQDSLASVYFLLLLIDYQIFRYW
                     GGGIGYTLMYTTLFLLLLSALLFSYKILLQLEQKEASWITAFFLFFNDFKSALFYLAG
                     TLILLVAFWFAGPIYFALLGFSFLFYFQVLLFIHRTQEKVLKKEE"
     gene            complement(1236..2090)
                     /locus_tag="ACG8PO_001539"
     CDS             complement(1236..2090)
                     /locus_tag="ACG8PO_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0206856.1"
                     /translation="MMDYFKKTSVNRQTKKILSYVLMTVIGIILIIPLLWMVFTSLKP
                     MEEIVRYPPTFFPEKIVWENYLDTIAAFPFWRYARNTLFITVLVVIGNVLSNSFIAYG
                     FAKLDFPGKKLMFALVLSTMMIPGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGNAFN
                     IFLMRQFYLSINNELIEAAEIDGANHLYIWSHLMLPLTKPALITIAINSFNAAWNDFL
                     GPLLYIQDQEKYTLQIGLQVFQNQATTQWNYLMAGATLVLIPTILLFFFAQRYFIEGM
                     DLTGGSKG"
     gene            complement(2087..2998)
                     /locus_tag="ACG8PO_001540"
     CDS             complement(2087..2998)
                     /locus_tag="ACG8PO_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0206857.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            complement(3039..4373)
                     /locus_tag="ACG8PO_001541"
     CDS             complement(3039..4373)
                     /locus_tag="ACG8PO_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0206858.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTDTQ
                     VIFYNKKLFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            4581..5411
                     /locus_tag="ACG8PO_001542"
     CDS             4581..5411
                     /locus_tag="ACG8PO_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HFQ0206859.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVAGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            complement(5655..5904)
                     /locus_tag="ACG8PO_001543"
                     /pseudo
     CDS             complement(5655..5904)
                     /locus_tag="ACG8PO_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206386.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(6095..6289)
                     /locus_tag="ACG8PO_001544"
     CDS             complement(6095..6289)
                     /locus_tag="ACG8PO_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0206860.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSCF
                     YTALFLGLLEAFSIYKKSKK"
     gene            complement(6794..6979)
                     /locus_tag="ACG8PO_001545"
     CDS             complement(6794..6979)
                     /locus_tag="ACG8PO_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0206861.1"
                     /translation="MSKNSKGFLTILLAFIGYMLVGLLKSYSNELLNFSTFINDTLVP
                     SLFFIVFFAVGYFIIKI"
     gene            complement(7829..8170)
                     /locus_tag="ACG8PO_001546"
     CDS             complement(7829..8170)
                     /locus_tag="ACG8PO_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFQ0206862.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(8170..8400)
                     /locus_tag="ACG8PO_001547"
     CDS             complement(8170..8400)
                     /locus_tag="ACG8PO_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFQ0206863.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
     gene            8687..9025
                     /locus_tag="ACG8PO_001548"
     CDS             8687..9025
                     /locus_tag="ACG8PO_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0206864.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(9230..9792)
                     /locus_tag="ACG8PO_001549"
                     /pseudo
     CDS             complement(9230..9792)
                     /locus_tag="ACG8PO_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349929.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - obsolete protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
     gene            complement(10524..11105)
                     /locus_tag="ACG8PO_001550"
     CDS             complement(10524..11105)
                     /locus_tag="ACG8PO_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0206865.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(11288..11914)
                     /locus_tag="ACG8PO_001551"
     CDS             complement(11288..11914)
                     /locus_tag="ACG8PO_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HFQ0206866.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(11936..13267)
                     /locus_tag="ACG8PO_001552"
     CDS             complement(11936..13267)
                     /locus_tag="ACG8PO_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016668 - oxidoreductase activity, acting
                     on a sulfur group of donors, NAD(P) as acceptor [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HFQ0206867.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(13422..13823)
                     /locus_tag="ACG8PO_001553"
     CDS             complement(13422..13823)
                     /locus_tag="ACG8PO_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HFQ0206868.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(13909..14115)
                     /locus_tag="ACG8PO_001554"
     CDS             complement(13909..14115)
                     /locus_tag="ACG8PO_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0206869.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(14210..15712)
                     /locus_tag="ACG8PO_001555"
     CDS             complement(14210..15712)
                     /locus_tag="ACG8PO_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HFQ0206870.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(15730..16185)
                     /gene="argR"
                     /locus_tag="ACG8PO_001556"
     CDS             complement(15730..16185)
                     /gene="argR"
                     /locus_tag="ACG8PO_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFQ0206871.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(16283..16900)
                     /locus_tag="ACG8PO_001557"
     CDS             complement(16283..16900)
                     /locus_tag="ACG8PO_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFQ0206872.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            17121..18494
                     /locus_tag="ACG8PO_001558"
     CDS             17121..18494
                     /locus_tag="ACG8PO_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0206873.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLFLSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            18569..19681
                     /locus_tag="ACG8PO_001559"
     CDS             18569..19681
                     /locus_tag="ACG8PO_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFQ0206874.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(19753..21390)
                     /locus_tag="ACG8PO_001560"
     CDS             complement(19753..21390)
                     /locus_tag="ACG8PO_001560"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HFQ0206875.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(21558..22166)
                     /locus_tag="ACG8PO_001561"
     CDS             complement(21558..22166)
                     /locus_tag="ACG8PO_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFQ0206876.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
BASE COUNT         6518 a         4539 c         3882 g         7376 t
ORIGIN      
        1 agttattaag acaaatgaat aaacaactac gctttcctcg aaacaaaaaa ataaagcaca
       61 aatgcgcatt tacgcactca tgctttattc gtacaattaa ttcagataac ggcagcgcat
      121 aggatgccaa taacacttcc taaaatagct cctcctaaaa tgtcagtagg ataatgtaaa
      181 agtaaaaaaa tcctcgaata agcgatcaaa caagcaaata aaaaggctaa tattcctgca
      241 actggactta attcgaacaa cgcagctact gataaaaagg ctagcgctgt atgtcctgaa
      301 ggaaaagaag aagtcttcgg tttacttgtg taaaggacaa gccgacgatt tttttcataa
      361 ggtcttttcc gtcttgtcgt ttttttgata aaaagcaaac caacaaaaaa atagatcaaa
      421 ctgccgataa gaatggctag accatattct ctagtatcag gtatcaagac taaaacagca
      481 gcaaggagca accaaataaa accaccatta gagatattac tgataaattt caaaaaattg
      541 atttttcgac tatccgtttt ccgttgcttg gcgaacttgt tgataatact atcatcgatt
      601 ttagcaatca aacgcaacat gttattcctc ctttttaaga accttttctt gcgtacgatg
      661 gataaaaagc aggacttgaa aataaaacaa gaaactgaaa cctaataaag caaaatagat
      721 tggtcctgca aaccagaatg cgacaagaag gatcaacgtg cctgccaaat agaaaagcgc
      781 gcttttgaaa tcattaaaaa acaagaaaaa tgcagtaatc caagaagctt ctttttgttc
      841 cagttgaagc aagattttgt aactaaagag aagcgcgctt aatagaagca aaaacaacgt
      901 agtatacatc aatgtatagc caattccccc accccaatag cggaaaatct gataatcgat
      961 caatagaaga agaaaataaa cacttgctaa actatcttgc tttccataaa gagagaagtt
     1021 ctcacgaaac tttttccaga atagtgaacc gagctttttc tcttcatttt ttttgtaatg
     1081 ataaatcata tacagaactg ttcgacttgc agttcctaat gtgaaaacag gcaagacgca
     1141 caggaaccat agtagctgta aatggataag acgtggaatc cattcaatga tttctaagaa
     1201 ataatcattg gcttttttac caatcgatcg attcatcatc cttttgaacc tcctgtcaaa
     1261 tccatccctt caatgaaata acgttgtgca aaaaagaaca gcaaaatcgt cgggatcaag
     1321 acaagtgttg ctcctgccat cagataattc cattgtgtcg ttgcttggtt ctgaaaaact
     1381 tgcaagccaa tctgtaacgt gtatttttct tgatcttgga tatataacag aggacctaag
     1441 aaatcattcc aagctgcgtt gaaggaattg atcgcaatgg taatcaaggc aggtttcgtc
     1501 aatggaagca tcaaatgact ccaaatatac aaatgattcg ctccgtcgat ttcagcagct
     1561 tcgatcaatt cattattgat cgataagtaa aattgacgca tcagaaatat attgaacgca
     1621 ttgccgaaga acgatggaac gatcaggggc aaataagtac ctacccagcc gatcttggag
     1681 aataatacat attgagggat catcgttaca aaccccggga tcatcatcgt agaaagaacc
     1741 aaagcaaaca tcagcttttt cccaggaaaa tcaagcttcg caaatccata agcaataaaa
     1801 gaatttgaaa ggacattacc gatcactact agcacagtga tgaataatgt atttcttgca
     1861 tagcgccaaa aaggaaaggc ggcaatcgta tccagatagt tttcccatac gattttttct
     1921 gggaaaaagg tcggaggata acgaacgatt tcttccattg gtttcaatga agtaaacacc
     1981 atccaaagca atggaatgat caaaatgatt cctattactg tcatgaggac ataacttagt
     2041 attttttttg tttgccgatt cacactggtc tttttgaaat aatccatcat ttgccgtcac
     2101 ctccagaata atagacccac ttaggtgcaa gcttcaaatt gacaaacgtc agaaccaaca
     2161 cgatcagaaa caggatcatt gacatagcca tcgcataacc catatcaaaa gctacgaacg
     2221 ccttgttcca catatgcaga ttataaaaaa gcaaagaatt tgcaggtccg cctgttccat
     2281 ttttcgtcat gacataagct tcttgaaaaa tttggaaaga accgatcgta gaggtgatca
     2341 catcgtaaaa aataatagga gtgattaatg gcaaagtaat acttttgaac tttccccaaa
     2401 cgcctgctcc atcgatttct gctgattcat ataaagatct aggaacattt tgtaatgtag
     2461 ctaagtaaag tagcattgct cctccagaac tccatatttt catcaaaatc aacgcaggtt
     2521 tcgtccattg tggatcaaac agccaagctg gtccagggat atggaaccag cccagaaatg
     2581 tgttgatcaa tcctgcagat ggagaaagca gctgcatcca taagatgtag actgctactc
     2641 ctgataagac tgccggtaaa tagaaagctg tacggaatac actgattcct ttgatttttt
     2701 gattgagtac tgtggctaaa aacacactga aaatcgtcgt cagcggtaca ttgaagatga
     2761 cgaaataaac ggtgttgtat aacgatttcc aaaataaatc atcgtgaacg aacattcttt
     2821 tgaaatttgc tagaccaatg aaattcatct tggatgtcaa attataatcg gtgaaactac
     2881 caataactga aaaaagcata ggccctagca taaataccaa gaacccgata ataaaaggcg
     2941 agataaataa ataggcccat cttgcttctt ttttctttcg gatagataaa cgcttcaaat
     3001 tttcttgcct cctctgtacc tagacagtca aaaatggatt attgattttg cttgactaag
     3061 ttttctactg ctttttgtgc atctgccaat ccttcttttg gtgattttgt tcctaaaaga
     3121 atctggtcaa tctgttcatt cactaatgtc gtgtaatccg gagcatctaa aggaactggt
     3181 gtgatcaccg tatttttaaa gttttcgtcc gccatttgat agatcatttt tcctttatcc
     3241 gttaggttgt cattattcac taaattctca ttggctgtct tgctcgccat gatatcaaaa
     3301 cttttttcgc cgaatttttc ttgtacttct ggagaagaaa gatatttgat gaattcataa
     3361 gaggcctcag gatcttttgc tccgtatggt acttccaaga cgaatccgcc accccacgac
     3421 caatggcctg aaccttcttc tttttctggg agctgcatca cgccaaaatc aaaatcatcc
     3481 ggtgcatttt ccatcaaact agagtaataa ttgatatttt gggcacgcat ggctactaat
     3541 ccggaaataa atggatcaga tacaccagac ccaaattcgg cttctaattt attgatcgta
     3601 tttttcccat aataatcttg ccagtcttgg atccactcca aggcttccac tttattttta
     3661 gtatcaatct tgactttttc atctttgtca aaccaactag tcccgttatc tgcgtttagc
     3721 gcccatacat cagcccctaa attccataat ggataaaaac cgatgcgtgt aaattctttt
     3781 ccttcttttt tatctagttt acgtgctgtt tcttctaatt catcccaagt tgtcggtaga
     3841 tcttgttcac tgattccagc ttctttaaat aactttttat tataaaaaat gacttgtgta
     3901 tctgtattga atgggatcgc ataaggatca ccttcatatt ctactgtatc ccaaagctgc
     3961 ggataaaaat ccttagaagt gtctttttct acatatttgg agagattagt tgcttgctgt
     4021 gcttctgctc tttgggcaac ggaatttatg tcctgtacaa taacatcggg tgcatttcca
     4081 gctgttattg cagataaaga tttagtccag atgtcacccc atggctgata cttataattt
     4141 acatggatct cgttttgtga ttgattgaaa tcctcaatga tttcttcgat agcttctttt
     4201 cgtgcaccgg atccccaaaa ggaccagaaa tcaatatttt ttcttccatc acttgttttt
     4261 tcagaagaat ttgctgcact tgaactggca tcgcttgcat tacttgatcc gcatcctcct
     4321 aataaaaaca atgctgaact tgccagcaac cctgtaaaca ataatttttt catattttac
     4381 ctccaaattt taatgtaacc gatgccggtt ttaatataac gatatcattt tgttctgaaa
     4441 ataacaattt ataaggtatg attctaaaaa aattattaat gataccaata ctttatataa
     4501 ttcgcgagcg ctttctttga attttttaat ctttcgtagc agaatgagaa tgtattaaaa
     4561 atagattagg aggaattttg atgtttagac accagaaaga attgcagttt gaagcaaaac
     4621 cagatcgtcc gaatccatta atcgcgaaat atctgcagga gctgatcggc ggccaatatg
     4681 gcgagatgag tgtagctatg cagtacctgt tccaaggctg gagctgccga ggagaagaaa
     4741 aatataaaga tatgctgatg gacattgcaa cagaagaaat cggccacgtg gaaatgttgg
     4801 cgacaatggt tgctcgttta ttagagggtg cgcctgttga agatcaagaa gcagcgatgc
     4861 aaaaagatcc aacaatcggt gccattatct ctggaatgaa tccacagcac gcgatcgtag
     4921 ctggcttagg acctcgtcct gctgacagcg tgggcaatcc atggagcggt gcctatatca
     4981 ttgcgagtgg caacttgtta gcagacttta gagctaattt aaatgccgaa tcacaaggaa
     5041 gacttcaagt agcacgtatt tacgaaatga tcgatgatcg tggcgtaaaa gatatgctgt
     5101 ctgttctcct tgcaagagat agctatcatc aaaatctatg ggaatgtgca gtgaaagaat
     5161 tagaagaaaa agaaggttca atcttagtcc caagtacttt cccgcgtgac aaagaacgaa
     5221 aagacattgc ttatgacttc tataacttct cagagggaga ccaaagtagt gaaggttctt
     5281 gggctagtgg caaagcactg gatggcgaag gcgaatatca atggttgaaa gaacctaaga
     5341 tcgaaggcgc tgcaccaaaa ttagaaccag taacaccaaa aatgtatggg acacctccat
     5401 tcaagaaata aatatctacc cgtagaaaca cccgtttttt gttccacacg ctttgtgagc
     5461 aaaactgact aaagtcatta atcataacca cccttcaaaa gggtggtttt ctctagagct
     5521 ataagctcaa atcaccggcc agcgcctaaa gacgctggct ttcatatttt atgttcaagc
     5581 catattgcct ttgccagttt tgcccacctc ttaaaaggtt tatgtactac tccttagccc
     5641 ttaaaaggat cgtcttactc tcgtacactc agcttatcta acgcactatc atgtttttct
     5701 tgttcttgaa tgtacttttt gatggtcgct tcattttgtc ctacttttgt cacatagtat
     5761 ccttccaccc aaaagtgtcg atacccaaat ttatatttta gatttgcaca atctcttgat
     5821 gatctcctga atattggctc gatattgatt ataaattatt tttcttctat actttggtgt
     5881 gaatacaatg tggtatttac acatctactt tgtacgggct aaactatttc cctttattgc
     5941 tccacccgca tagcaagtgg ttttgtattt caatcgctcg gcgcgctcaa ttaggtctaa
     6001 agacaataaa tctaaaaacc cgctgcttca ctgacagaaa caacgggttt tcagaaaaaa
     6061 atgaaatgaa aaagatctct tacggcaatc tattttattt ttttgatttt ttataaatgg
     6121 aaaatgcttc aagaagtcca agaaataaag ccgtataaaa acaagaacct atgaaatctt
     6181 tatttattag atattcaaca gaaatcccga taacactagc caacaatata tttatcagaa
     6241 gtgtaaaata aggatgcttt ttatagaata gaactagtgt tgttttcata gactctcctt
     6301 tcttcatatt tccaattaat cacaaattca cggcattact agaaaaatta aaggggcgca
     6361 gaagtggaaa aatactcttc gactttccct acaaaccagg ccatcttgtg tttaataact
     6421 ataccaaatc taaggtacct ctaaatctaa attccatttg tggcggtgac ctccaatttt
     6481 taactcttct atctcccatc cagtatacat aaccttgata cttcgaccac ttatatatcg
     6541 aacacacaaa tacctatcca ttgcaggagt actacctaac ttagttgcta cttgcatcta
     6601 aataacagct gaaaagatag gacttactct tagtaaagta tctgaactat taatttttaa
     6661 actatcattt ttatcctcaa ctacatgcac atctgttgca acttcacttt ctgtctcatc
     6721 cagataaatg attaaggaat cgggaattgc aatatcttca gctgacgctg gaatagttga
     6781 gctaaattag tcatcatatc tttattataa aataaccaac agcaaaaaat acaatgaaaa
     6841 acaaacttgg aactaatgta tcattaataa atgttgaaaa attcagcaac tcattactgt
     6901 atgattttag cagcccaacc aacatatacc ctataaaagc taataagatg gttaggaatc
     6961 ctttagaatt tttactcatt catagacact tccttaaata acttttatta tagaaggtat
     7021 tgtaaataat aatattgaca aaaagtgact caaaataaaa aaaaccttac attatactgg
     7081 aatgaactgt attgatagtg tacctaaaag ctctactgac ataagatcgt gtaatcggta
     7141 aagtaatttt attaattata cgggatgtaa tcaattagag aataaactaa tcataaaagg
     7201 tacttttaca ttcataattg tcgctttgag attctacttt ggttcactat ctgacccaat
     7261 tgcacatttt cgatcattac aagtattggt gtcgttagaa caactaaata aaaactagcg
     7321 gtagattttt cagaatctat gctaatttct gtatggatgt tccagtgatg gcaaatttct
     7381 cttttcattg tttttcttgt cgcatgatat gcctttttgt gttattcagt taaactatag
     7441 aaattctttc ctagacagat cttgatccca aattttacgt ttttgagcat cgatatagtt
     7501 taaaatgaca ttactcatat ctaaagagtt tagatttttt aggactaaaa ctgtcagttg
     7561 ttctttttct tctttctgtt aaagtattgt cgcgactttt cgtatacgtt aagatatgtc
     7621 gtgaaaaaac aagttttgtg acacaaaaaa acgaatgact tgaggaaatc acctcaatca
     7681 ttcgtttaaa atgctatata ttctttagtt taagatacgg aaattaatgc catcgaccac
     7741 actagtagaa tgaacgacat ttacttggcc tttttttaat ttcgttatgt ttagcatata
     7801 aaaatattat agagtttact tacaatgctt aaaacatata ctgtataatc tgatcaattt
     7861 tagccatgtc ttgcaatggt aagttttcta tttttttgag ttttcgttcc ttaaaatcaa
     7921 gagattttaa ttgtgtaata attacttgtc cttttatatc tagctcttca ggtaatgagt
     7981 aacgtgtcgg taaattcttt atagtggaag taattggaca tatgaccgaa aacattgttt
     8041 tacggttaaa ttcataacga gagacgacta gtcctggtcg tcttttttga atttcttttc
     8101 ctacggaggg atcaaaatca atccatacaa tatcaccttt tttaggaata taaattcttt
     8161 cttctgacac tataaaattt ctctcccttc aggagttaaa tcttcccatt cgtctttctc
     8221 ataatactcg gcctcttccc caccactaaa tggatcttca atttttggaa ctaacgtaat
     8281 tgttccatca tcagaataca caacgatata ttcttggttt tctgaaggtt tctttccgtt
     8341 atcagaaggt aaagttacca ctacagaact tccttgtaat ctagattttg taacaagcat
     8401 tatcatcact cccttaataa aagtatacta tataacacta agtaacgcaa aattcccctt
     8461 tgtaaactat ctagaaaatt gttttgttta aagtatatct tattggatat aaatagaaat
     8521 aaattgtagc cagttcattt tttctttaac taaaaaaata atccaagaaa cattgaacct
     8581 atggatccaa aagctaccca aatactatag ctaacccccc gctaccttca attttgcaga
     8641 taaaacgctt tgggagaaaa tttcaaagac aacaggccat cctatcatgc ccgattataa
     8701 acaaaaagct ggctattcct tttgctctga caaatttcaa ttgggaaagt tcacgaaact
     8761 agcaggtgaa gaagtttcaa ctgttcggat caaagaatgc ccgcttcaaa tagaaacatt
     8821 ggttcaacga atttcaacta gacaagactt tgctattgta gaacgtgaga tcgtagctat
     8881 ttttgtagaa gaagggattt tacatgatcc aactcatatc aatgtggata aatggcaacc
     8941 tcttatctat aaattcagag aatatacaac tgccaaccag tcgttaactt tgaattttag
     9001 attccaagaa ttcaaagagt cataatgata aacatcgtta ggtataaata aaaaagactc
     9061 gcagcacgag taaaaaacaa atagaagcta tacattagaa aggtcgactg tgaccctata
     9121 gatgcagtat atgcagctta tgagcatttc ttctgttctc tttgtttttg ggatctgtgc
     9181 tgaagtcttt ttcgtttttt gccgctcttt ctttgaaaaa tcgattatgt tataccttct
     9241 ccattactaa ttcaggcatc tgctgagtaa tgatttttcg cgcaaaagaa atatctgtta
     9301 cccaaggttc gatttgttct tcagtcaaga caagcggcat tcggtcatgg attggtgcga
     9361 ctgtgcggtt aggttcagta gtcattatga ttgattctgc cattttttca tcctgatggc
     9421 tacgataaaa accgccaata tacactattt tttccttatt tgaaaacata aatttgtctt
     9481 tttcttcatt ccactcattg ggaaaacaca acgattttgc aaaaatggtt gatgaaaggt
     9541 ctttttttct tctactgtct ctgcacgggc attaatcatc aattgtcctt ttttaaatcc
     9601 ggtaaatccc cacttcatga tttccggagt gaggatgttt tcttcctctt ttcttaacgt
     9661 cacaacaaga tttgacggaa aaacttctcc agctgctaat tctgctgcct gcttcggttg
     9721 tttcgtctgg gcttctcgat aataattctg aaggagctct gactctagat caaaaaaata
     9781 acgaccacac atcctaatcc ctcccaatct ttttttctat catacactgc aacaagaagt
     9841 tcgcaccaac aaaacgcttt caatttttgt agaactaaaa aagttgtgac aagtgacaaa
     9901 ctctccagtg aatggtattc tcctgccaac tcctcggtca caagtcacaa tattcaacaa
     9961 acatgagaag ctgctttttg aaattgttcc ttgtgactcg gagtgaaacg gcaggcttac
    10021 gacctctggg taaacggtgt tcaaaagcca gtttctcggt attaagccaa atttatgcaa
    10081 aaatgtagaa agctattttc acaaaatttt tcttaatact cgaaactaag cgactttttc
    10141 acaacctctt ccttaaacgg tgttcaaaag cttgcatctg cggtaataag cccaaccaaa
    10201 cgaaaaatat gagcagctat ttttatttgg ctgttcttat tacttgatgc agggcagctt
    10261 tttcacaacc tctccactaa acggtgttca atcagctaac cttcggtatt agctcaaaaa
    10321 acggcaaaat atgaggaact attttgccgt ttgtttctct aatactcagg tcaaagcgct
    10381 gatttcacaa cctctaatac acggtgttcc agaagtagct tcttcgaaaa ataagccgaa
    10441 atttcacaaa aatttgagaa gcaattttcg taaattcctt cttatttctt gaagctgacc
    10501 acttctgtca caacctctaa ttattaaaga accttttgcg aagttaacaa tctttctttg
    10561 atcaattgaa aattctgcct aagtagtgat agatcctgtg tgactttcaa caataagata
    10621 cttccctgga tttcagcaat caacgctttg gcttgacggt ctgcttcttc atcggacagt
    10681 ttttcttcag aaagtgccag gatattttgt ttcgtatact tgacccattg attgactaat
    10741 tctagtagca acagacggta ttcttcatcc actgtactca actcaataat caaattacct
    10801 acaggacagc catgataagt gatctgtgtc tcatgatcag cgaaaatcca gtccaacatt
    10861 tcttctaatg cttcttctgg ctgttttcca gcatttaaga tgtcttcgaa caattctgtc
    10921 tgccatacgt ccatatgcgc atggatcacc gctagacaaa cttctttttt tgaagaaaag
    10981 taataataaa attgaccttt tcctgtatca gaagctgcta gaatatctcg gattgacgtt
    11041 tcatgatagc cttttttata aataagatct ttggtcgctt ccaagatctt ttcctttgtt
    11101 ttcaatgata ttctcctttt tcatttctca tagtcctata ttatacagta aattcatcat
    11161 tttttcttct taatcgttta aaaaagactg tgccaaatta tctgacacag ccctcactat
    11221 aaaactgttc tcatatagct agatgttcat tgattcaaga actgcgagga caaaagcaat
    11281 tcctatctta tgatagttgc tctaatactt cctctgttgt catcacttga tctgcaataa
    11341 agccaaagtt cgtcaacgct gcatccattg cttcttggcc aggagcacca attgcatctg
    11401 tcgctaccac aacattgaat ccactctcta ccaattcacg catatgggaa tcgctacaca
    11461 agtttccgtt cattccacca agaattactg tgtcgattcc attttttctc aattggagcg
    11521 ccaaatcatt gctttctggt ccaaagattt tatgaggaga gctgatgatc gtattttcat
    11581 ccaaataagg tttcaattct tcgataaaat cggctcctga accttcttcc actgattcat
    11641 attgactttt tctagcaaac atctgctgct caagcatcat atgttctcct gcaccgccaa
    11701 attgccattt atggtcatgt gggtaaaaat aatgaggaga aataaagagt ttgtatcctt
    11761 tttcttttgc ttgttttacc atttcaatca tattttcgat cgtattgtat ttttctaaga
    11821 tatcttttgt caaaccaaag ccaccgcctg ctggtttcaa gaactcgact tgcgggtcag
    11881 tgagaactac tgctgtattt ttattattga acatggtgga ttctccattc gttatttatt
    11941 ttaatacatc atttagagct tcactcattg tcggatgaga atagatttga tctctcaata
    12001 acgtataagg aagttttcca ttcatagcta gcgaaatcaa gttgatgact tcatgagaat
    12061 cttccgcata gatacttgcc cctagaatca agtcagtttc agggtcgact aaaactttga
    12121 atacaccttt aggatcttct aatacttgtg ctttagggac accgcttgtc atatacttga
    12181 ataatttgta attgacgcct tgtgctttgg cttgtttttc tgttaaacct acattggata
    12241 aagtcggagt gataaataca ctgtaaggca cagctttccg gttattcgtt gttcgtttat
    12301 tctcgccctt caactgatcc ataatgattc gataatcatc tagagagata taagtgaact
    12361 gcaatccgcc tttgacgtct ccaatcgccc aaatgttgtc tgcatctgta cgaaggtatt
    12421 catccacagc gattgctccg cgatctgtta gtttaacatc tgtattttct aatccaagtg
    12481 atgctgtact tggttttctt ccagttgcag ccaaaacttt tgacgcttgg attgttactt
    12541 cttggccatc ttctgtaaag gtgacggcag ctgttgattc ttggtctgct acttgatcta
    12601 cagatacacc tagatggaat tcgattcccg catttgtcat gtcttctaaa atcatttgcg
    12661 cgatatcttc atcttcacgt ggaagaaatt cagaatgggc atcaagaacg acaactttag
    12721 atccgtattc attgaacatt gatgcaaatt ccaagccaat atatccagcc ccgatgatca
    12781 ctaatttttc cggtaattgt tcctgatcca tcgcttgcgt agaatcaatc aagtagcgac
    12841 ttgtattcaa tcctttgatt ggtaaaatga ttggttcaga accagtattg atgaaaatcc
    12901 gttcgccttc aacaatcact ttttcaccat tattcgtcac ttcgatttga tggttagaaa
    12961 gaaaacgtgc tgtaccgtcc atcactgtcc cagttgcctc atcgctgatc atatgataat
    13021 ttttggcgcg aagcatgccc gtcaattttt ctttccgagc aacagcctct gtaaagtcga
    13081 ttcctctttc tccattgaaa atcaatgatt tagaaggaat acagccgatg ttgatacatg
    13141 ttccgccata catacgtgtt gacttttcaa cgactaaaac cgattcacct tttgaagcta
    13201 acgccttcgc taaagtctta ccgccctttc caaaaccgat cacaatattt tgatattttt
    13261 ccatcatttt tccctcgctt cttttttacc taccgattgg tacaaaaata tgataaccga
    13321 taattgtcta ttctccaaat catctgcttt atttgtcaaa aaaaaccaga caaaaagcgg
    13381 tcctttcacg tttttagtct ggttcgattt cctttattct tctaaacaat cgttacttct
    13441 acatcgatat ttcctctagt tgctttggag taagggcaga attcatgcgc atcttctgca
    13501 attttttggg cttcttcttc gctgatgcca tcgatatgca cttctaatac tacaccgatc
    13561 tgatagccgc catcatctaa attataaaga gagacacgtg cttttactgt ggactctgct
    13621 gtcattcctg cttctttgat catatgttgt accgccccat tgaaacaaga agcataacct
    13681 gctgcaaata attgttctgg atttgttcct tctgcatgga ttcctggagg agtgattttc
    13741 atatgcatcg agccatttgg tgcctcgact tctccattgc gcccgccatg gttgatcatt
    13801 gctgtttcgt atgctttttt cattgtactg ctcctttcga ttgaccattt gctacttggc
    13861 tataattata gtgtatgaca agtaaggcgc tttctacaaa aattctgctt attcgaatac
    13921 cagaatgccg tcttctacta tttttccaaa aaacaaaaaa atactactcg tgaacaacag
    13981 cgtcatattt gttccaaaat aaatagcaat cttttctaca taatctttta tatgattgat
    14041 tttaagatac atatatacca gaaagttgac cccataaacc aatactgtta aaaaaataac
    14101 catattcgtt accatttttt cctccttttg ctggtctccc taccagcact cgatttaacc
    14161 ataccacttt ttttgccgtc caagcaacta gcctttcaaa aagactggtt tattctgctg
    14221 ttaatgattc aagatcagaa aaatgtccga ataattttgc taattttact aacgccaact
    14281 tgttctttcc gaattcatca ttttctatct gtaatactag taacggctca tgtaagctta
    14341 aacggatcaa aaaccagccg tttccgtatg gctcttctag atcatatcga ataccttctt
    14401 cattttccgg gtcgaagttc atcccttttt cttcttccaa cagtcgcttc atttcttcta
    14461 atatcttgtt tccttgttcg cgtggttcat cgctgaacaa ttttaatcgg atttcttgta
    14521 cttctacggg ctgtttcaac tctttgattt gttcatctaa tgtgatacct tttcttttta
    14581 attctggcaa aagcatcagg atctttgcga tgacatatgc accatcatcc agaaaatagt
    14641 tttccttgaa tgcagcgtgt ccgcttgttt cgatggctag ctgtgtatct ttcccaagac
    14701 ggttaagttc taatgcttta ttgattacat ttcgatagcc ggaaatataa cgaacttgga
    14761 tgcctccttt ttcttcaatg aatctttgta gatgagaaga agtaggggaa ttcgtcacga
    14821 ttcttgttcc tggatgctct tttaatacga ttgtacttaa tactgcgatc aaattatttc
    14881 gattgatcac tgttccagaa gcactgacaa ctgcagaacg atccacatct gtatcgaaaa
    14941 tgatcccaag atctgcttca tttgctaaaa cagcctgctt gatactttcc atcgcttctt
    15001 tattatcagg atttgggata tgattaggaa atcggccatc tggttccaaa aattgggaac
    15061 ctgttgaatc cgcacctaag ctattcaata ctttttcagc aaagaatcca ccagcaccat
    15121 ttccggcatc aacaatgatt ttccaaccat ttagtggttt gctttctagt gttccaatcc
    15181 cctttttaat ttttgtgact aaatcttgcg cataaggtgt caaaaggtct gctttttcta
    15241 cgcttccagt ttgttcgctt tttatttttt gctgctgtga taggataaaa gcaatatctt
    15301 ctttttctgc tcctccttct gaggtaaaga attttattcc attataataa tagggaagat
    15361 ggctagcagt cagcataacc gcagcatcgc acgagaactg ttcaaattga gtggacataa
    15421 acatagcagg cgtagtagct aattcgaaat cgatgacatg tatcccttgt tctttcagac
    15481 tttggatcaa cgcctctttc agtgctggtc cgcttatcct actgtcatga ccaatcccaa
    15541 tcgtccattt tttatttgga tcggttgatt tcttcttttt ctttatccag tttgctagtc
    15601 cccagccgat cttttttacc atgtctgtcg tcaagtttac ttcatatcct tcaactggta
    15661 atgcgattcc tcgaatatcc gatccatttt gcagcgcttt catatctttc atataaaagt
    15721 cctcctcatt tattccctca ttttttccag cttttctgca aatcgctgtg cttcttcttc
    15781 tgagcggcaa ataataatga atgtatctac accggccatc gttccgacaa cttcagggta
    15841 agccacttca tctagccaat tcgttaccac atcggcacca tttcgttcag tcaatacgat
    15901 aaccatgaac tgaacagact ggatacgcaa aacctcgcgt ctaattgctg tacgcaaacg
    15961 ttcttcattt aaagaaacag atggctggtt aaataatgta tagcgtacta ttttgttttc
    16021 attttgactt tttactaact tcatttctcg aatgtctcga gaaatcgttg cttgagtcac
    16081 atcggcgcct gcttcatgca gccgcgcaag cagctcttcc tgtgtcccaa taggttgttc
    16141 tgtaatgatc tggcggatca gtgcttgtcg ctgtttcttt ttcatattag acacctctca
    16201 atgttgatta tagcaaaaga taagtaatat ttcttcatat tttggtattt tttactttta
    16261 ttttttgttt tcattcaggt tgctaacctt tttctatttt aacacgcaaa atcgtcttca
    16321 ttttttcagg tgctgatttt cttggatcgc tgatgtaaat ctctttgtgt tctttagaag
    16381 ctctttcata gccttctttt gcgatgaatg cttctaattt ctcaaacgtt gctggttctt
    16441 cggcataggg cccgacgtgt aagatttggg cacaaagacc ttcttccatc tttttgaata
    16501 ctacttgttc tttcaagtct tccggaagtt tgctagaacg ctgaatggct tcttgtgcta
    16561 tcgcttctgt aacaaagtca ggctgtcgga tcatcaattg ataagaaaaa tgttcttttt
    16621 tcatcactgg ttcatgcaaa tacttttctt gcagtcccca ttgtccttcc agtggataga
    16681 cagtatacat ctgataatca ggaatcagcc aatttttttt aggcgacatg cgtattgcat
    16741 agctgactgc atatagacag gctactcttc gtttaaagtc ttcctgattc gggtcgccga
    16801 tccctctgat gctaaagaat gattttttag gaacctctag tatttgcggg ctttttttca
    16861 tactatagct cttgtcttta cgccattcaa ttttttccat ttttcttcct ccgcgaaatt
    16921 ccatttagtt atttattatc atacctcttt ttgagtgact tggctaatcg tgttgacact
    16981 atttttcagt ttgtgctaga ataagtgaag taaataaatc gtacagataa cacgtttcgg
    17041 cgtgtcgaga gagaggcggt ttctattttt ataatagaaa tcactctctt tttttacgaa
    17101 attttttaga ggtgatgtaa atgacaaaac aaaaacagct gcttttagta cctgcagtaa
    17161 ttgcaacagg gatgatgtct tttgcgggcg tattgatcga gacggccatg aatgtaactt
    17221 tcccaactct tatcagacag tttgatttaa cgactgcaca agtccaatgg gtaacaacga
    17281 tttatctttt gatgatttcg atcattgttc cgctgtctac ttatttgaat cgcaatttta
    17341 gtctcaaaaa actcttttta gcttcaaatc tgcttttctt ggcaggtata atgatcgatt
    17401 tcttctctcc taccttcatg gtcctattgt ttggtcgatt gctccaaggt gcagcgacag
    17461 gtatcgctct tccgttgatg ttccatatta tcttgacatt tgcccccctc aatcgacgag
    17521 gcgcgatgat gggtgtcggt actttgacaa cggccattgc tccagcaatt ggccccactt
    17581 atggaggcat tatgacttca cattttacat ggaatcatat cttcctgttt cttgtgcccg
    17641 ttttgtttct ttctttgatt atcggctggt ttgctattcc cgagatggca gtacaaaaaa
    17701 gtggttattt ggattggata agcgttttag gaatttgtct tttgtttagt ggaagtctaa
    17761 catttttgag tacgttggga acagtttcca gctggattgc tttaatcatc ggcggtattg
    17821 gctggttcct tttttactat cgaagtaaaa aagtggagca tccactgatc cgactgtcta
    17881 ttctttcaaa taaaacattc cgcttgtttt tgattggctt cttagtttat cagtttttat
    17941 tacttggtgt atcttttgtc ttgccgaatt tccttcagat cgttcaagga agtagtcctt
    18001 ttgtcgctgg attagcgatg cttcccggcg cagcggttgg tgctttgctt tctccttttt
    18061 ccgggaaaat gctggatcaa tatggtccga agaagccaat ttttgctggg ctgattcttt
    18121 ctttgattgg ttggctagca ttattcctac tcataggaca cgcttcagtg ggtgtattga
    18181 ttgcttgtca tgtgttttat atgatcggta tcggattatc ttacagcaat atgatgacca
    18241 ccggaatgaa tgcactgaat gaagaactac aaggagacgg gaacgcaatg ttcaatacct
    18301 tgcagcagtt ttctggcgct gtggctactt cattagtagc ggtcattatc aattacgtcc
    18361 agcatcatac ttcccataac tatgaagtgt ctactacatt aggatcaaaa gcagcattag
    18421 gcgtcttgtt gctgttgctt ctcgtaagct ttgctcgatt cgcctattat ctgttctttg
    18481 ctaaaaaagc gtagattcca tatacggcag gtgttgcatc tgccgttttt ttgtgtgaaa
    18541 atggttgttt gtaaaaggag gcttttttat ggaaaaaatt gccattatcg gtggcgggat
    18601 cattggtatg accctagcca gttacttaga tacatcaaaa ttcaatgttg ctttatttga
    18661 tgaagggaca ggacaggcaa ctaaagctag cgctgggatt atctcccctt ggttgtcgaa
    18721 acgcagaaac aaacaatggt atcgtctggc taaagacggc gctgcttttt tccctaaact
    18781 tataaaagat ttccaacttg atcaaacgat ttacgcacaa tcagggacac ttttgcttcg
    18841 acctgaaaaa gatttagctg aattggctgt tcttgccgag aaacgaaaag aagaagcgcc
    18901 agaaattgga aacatcgaat ggctttcacc tgaacagaca tctcaagcac ttccgttatt
    18961 gaaaccagca gtttcactgc gtatcagtgg tggtggtcgt ctggatggaa aagcatattt
    19021 agaacatctt ggtcagcgtt tagcacaaaa acaaatggag cagatccagg caaaagtcac
    19081 tttgcataag aagagcgatt cttggctcat caagggaccg tttggagaat acacagctga
    19141 tcgtgtaatt ttgacacctg gcccggcact taagccttta ttacaagaat taggctatgc
    19201 tgcagatata cgtccgcaaa aagggcagct cttagcattt cagacatctt ataagatgag
    19261 tcaagactgg cctgttgcta tgcttgacgg agaagctgat ttcattcctt tcatggatgg
    19321 aaagatcctt ttaggtgcta cccatgaaaa cgatcaaggc tgggatttgg ctcctactag
    19381 cgaagcttat aagggattga ctaagagtac agaacagttc ttgactgatc cagatcagat
    19441 aaaaaactgg ccatacgagt tccgtgtagg tacccgtgct tatacgtcag atttttcccc
    19501 atttttcggc gtactgccag atgatccaag cttactggct gctagcggtc taggctcttc
    19561 tggcttgacc acaggtcctt atatcggcta tctattagcc aacttcttaa atactggtca
    19621 ttggcctacg gaagattatc aaaaggacat ctccacctat attacgaaaa atccatcata
    19681 gaaaaacagc tttttcaagt aaggatcccg agcgatattg cgcatggatt acttcttgaa
    19741 aaagctgtta ttttagttca atttctgaat caaattctcc aaactttctt ttgcttcatc
    19801 tggcaagaga tcttttccta tctgagcttc aatgaatcgt aaacgaatat caagtcgttg
    19861 tttcaccaga tttttatagg acgtatcctc aaaagaaaca ggaaaatctg gtataggtaa
    19921 atagtggatg tcttcaaatg gaccaaacga cttgaattct gccgtttgtt gcacgattct
    19981 ttcaacgatt gacaatgtcg tttcaggttt gattttttta tcatcgaaat atccagtatt
    20041 caggacatag caggctgttt gctgtttcaa aaacaactct cgaaaagctt gatagtcttc
    20101 cctaagagga tagacacgga aaggattagc aaatggttca atgactagct catctagctg
    20161 tctttttgta gtgatatttt ccgcgtctga tcgttttgtt gctaatgttg caccaaaaga
    20221 cgctgctaaa actgggtcat tgatcttgat aattggcgga agcgaatcat ctttcatgat
    20281 ccaaaaaaca gcatcgatct tttcgctaag atgatcgact cggtcgggtg tcgcataacg
    20341 tgatttgact gtccgcccat ttccgtttcg tatatctcct gttactagga ctttttctcc
    20401 tttacttgta catgtcacgc ccacattctg acaagtaagg aaagaagaaa tggccggatc
    20461 atcagacgga tagtcttgtg ttttatcaaa gtaagaaggt tccaaggcag tagcaaaacc
    20521 attttttcga gaaataacga aagcatcgtc atgcaagatc gaaacagagt gaccttccgt
    20581 atgttttgct aaggtaatcg ttgattttcc agaaccggac aacccaaata cagccatcgt
    20641 ataggtctgg tcattactag catattgttt catcccaccg tggcaggcaa taaagccatt
    20701 gcgatttccc aatgcccaag caagcgtcaa agtcgctttt tttaattctc caaagtaacg
    20761 taatcctagc acagccgctg tattatggag agcatcaaac aaaatgagtc catttggaaa
    20821 tgtttcatgt gtccattcgg gatcacaaaa gatataaaga tctccctcat caaaggaaac
    20881 agacttttga taagtctcta agatcttcga attcgctggc tgaaaattca gcaaataaga
    20941 atacaaattg ttctcaaatc cttctggaat cagcaaatga ctttgcacag aaaattcttc
    21001 agataggcca acgattactt ttgtatggta aaactggcgc ttgcgacttt gatacacagc
    21061 ttctcttaaa attttctcgt atttgctttg ttccttgtct aagtcctcaa agatccgccg
    21121 agcacttgct gttcttccca caatgcttcc atcattgaat actagtgtcc taacatcagc
    21181 tggtaaatct agttcttcag catgtgagat tgttaaatct gtgatgatcg ttcctggact
    21241 cccttttgct agttgataag ctgtcttcct atcaaaaact tcatgaacat tattaccgta
    21301 aaatgtcgtt tctacaatcg atcgaatact ggataacagt ggatttccac ttgtgatttc
    21361 gtttaattcg agactctctt ttgtactcat ggctcctcct cattccctaa acaaaaaaac
    21421 gttttcttta ttatacatta ttcgagtttt tatttgtact gctgcgctgt gggaagcaaa
    21481 cgagttattt ttcacacaaa aaaaccgaga acttgaataa acaagtttct cggtttcatc
    21541 attttccttt tatcggctta gtaccagccg tttgctaacc agaatgtttt agcagcatcc
    21601 catgaaccat aacgagatgt tacatattgt tctgctacac gttcttgatt ggcagctgaa
    21661 tagtcgccgt ttaagtatga agcatctaat tgataacggc cgatgtaacg tccgttagta
    21721 gctgtgtatg aaccgcctga ttctttttgt gcgatccatt cttttgcaga gcttgaagtt
    21781 ggcgctgttg cagcagctgg ggcagcagga gctacttcaa ctgtttcaac aggtgcaacg
    21841 tatgtatgtt cttgcgttgc tgtttcagtt gtagatgctg tattttctgt tgttttgttt
    21901 tctgttgttg ctgaagaatc atttgaagtt gggattgtta attgttcacc aacataaatg
    21961 cggttgatgt ttgcgatcga atttttttca gcaattgcat cgatcattga gttgtctcct
    22021 gcaaattttt gtgagatctt agataatgaa tcacctgatt ttactgtata aacttcatca
    22081 gcgtgagcat ttgtagttcc tacaaacatt gcagtagcac cagcagcaaa agttgtacct
    22141 aaaacaattt tcttaaatga attcatatta aactcctttt tttctccgtt ttttattctt
    22201 tcgacgctat taactttatc atttagaaat atttcatagg taaaagaaac atgacattta
    22261 atgacaaaat aacgaattat ttaatatttg taacatttaa acaaatattt ttttg
//
LOCUS       DAZKKW010000042        22281 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.042, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000042 DAZKKW010000000
VERSION     DAZKKW010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22281
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.042"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..636
                     /locus_tag="ACG8PO_001562"
     CDS             <1..636
                     /locus_tag="ACG8PO_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0206877.1"
                     /translation="ETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQI
                     GLLLNIVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTK
                     SLAKDVDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESL
                     GKQTGIMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            654..1559
                     /locus_tag="ACG8PO_001563"
     CDS             654..1559
                     /locus_tag="ACG8PO_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0206878.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGSAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            1556..2362
                     /locus_tag="ACG8PO_001564"
     CDS             1556..2362
                     /locus_tag="ACG8PO_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0206879.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            2519..3487
                     /locus_tag="ACG8PO_001565"
     CDS             2519..3487
                     /locus_tag="ACG8PO_001565"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HFQ0206880.1"
                     /translation="MTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKAIKELEKA
                     GYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDAKLAAAQGD
                     SVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTIIAASACVK
                     AIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLNFSVPQNH
                     FPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKSFVLGKTV
                     SFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(3804..4328)
                     /locus_tag="ACG8PO_001566"
     CDS             complement(3804..4328)
                     /locus_tag="ACG8PO_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HFQ0206881.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            4615..6312
                     /gene="scm"
                     /locus_tag="ACG8PO_001567"
                     /pseudo
     CDS             4615..6312
                     /gene="scm"
                     /locus_tag="ACG8PO_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
     gene            complement(6467..7453)
                     /locus_tag="ACG8PO_001568"
     CDS             complement(6467..7453)
                     /locus_tag="ACG8PO_001568"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFQ0206882.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(7476..8042)
                     /locus_tag="ACG8PO_001569"
     CDS             complement(7476..8042)
                     /locus_tag="ACG8PO_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HFQ0206883.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKRDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            complement(8056..8472)
                     /locus_tag="ACG8PO_001570"
     CDS             complement(8056..8472)
                     /locus_tag="ACG8PO_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HFQ0206884.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            complement(8929..10863)
                     /locus_tag="ACG8PO_001571"
     CDS             complement(8929..10863)
                     /locus_tag="ACG8PO_001571"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFQ0206885.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            11165..12100
                     /gene="pplA"
                     /locus_tag="ACG8PO_001572"
     CDS             11165..12100
                     /gene="pplA"
                     /locus_tag="ACG8PO_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular electron transfer flavoprotein
                     PplA"
                     /protein_id="HFQ0206886.1"
                     /translation="MKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSESSTAQSST
                     AKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKYDNVNENG
                     ESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSSESFQNYA
                     QQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGKVTESNFD
                     YIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPANVEVVTGA
                     THSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            12190..13293
                     /locus_tag="ACG8PO_001573"
     CDS             12190..13293
                     /locus_tag="ACG8PO_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HFQ0206887.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            13306..14259
                     /gene="menA"
                     /locus_tag="ACG8PO_001574"
     CDS             13306..14259
                     /gene="menA"
                     /locus_tag="ACG8PO_001574"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HFQ0206888.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFALVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(15073..16419)
                     /gene="gor"
                     /locus_tag="ACG8PO_001575"
     CDS             complement(15073..16419)
                     /gene="gor"
                     /locus_tag="ACG8PO_001575"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HFQ0206889.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFCGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(16553..17806)
                     /locus_tag="ACG8PO_001576"
     CDS             complement(16553..17806)
                     /locus_tag="ACG8PO_001576"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1901576 - obsolete organic substance
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HFQ0206890.1"
                     /translation="MMKEMTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAY
                     GLRSRAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISA
                     VIVSDPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVA
                     EIRKNTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFG
                     QERNSLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNV
                     YKAAVDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVY
                     KFVGEVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRA
                     PNPMMILTMPVAQPVKPGDMIRKRK"
     gene            complement(17812..18735)
                     /locus_tag="ACG8PO_001577"
     CDS             complement(17812..18735)
                     /locus_tag="ACG8PO_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HFQ0206891.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(18956..21448)
                     /locus_tag="ACG8PO_001578"
     CDS             complement(18956..21448)
                     /locus_tag="ACG8PO_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HFQ0206892.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(21455..21922)
                     /locus_tag="ACG8PO_001579"
     CDS             complement(21455..21922)
                     /locus_tag="ACG8PO_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HFQ0206893.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
     gene            complement(22058..22131)
                     /locus_tag="ACG8PO_001580"
     tRNA            complement(22058..22131)
                     /locus_tag="ACG8PO_001580"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(22095..22097),aa:Arg,seq:acg)
     gene            complement(22189..22262)
                     /locus_tag="ACG8PO_001581"
     tRNA            complement(22189..22262)
                     /locus_tag="ACG8PO_001581"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(22226..22228),aa:Pro,seq:tgg)
BASE COUNT         6671 a         4664 c         4086 g         6860 t
ORIGIN      
        1 gaaacaacca ctatcccgat gactgtcaca gcggtcacag acttggaaca agcccattta
       61 gtcacatcca atgaaaaacc tggcggcaat gtgactggca cgacagatat ggtgccaatc
      121 gaaaaacaaa tcggcttact gttaaatatc gtgccagacg ccaaaacaat cggcatcatg
      181 tataacaacg gtgaagcaaa ctctaagatc caagctgatt tggcagaaaa agaattgaaa
      241 aaagcgggcg tcaaagtcaa agtactgaca gcaaattcca caaatgatgt ccaacaagtg
      301 acgaaaagtc tagctaaaga tgtagatgga atctatattc ctactgataa cacgttcgca
      361 agtgcggcaa gtgttgtcgg tgaagtcgca aaagaaacaa aaacacctgt cgttgctggc
      421 gcagttgatc aagtcaaaga cggcggtcta gcaacagtag gaatcgacta cgaaagcttg
      481 ggcaaacaaa cagggatcat ggcagcaaaa atcttagacg gcaagaatcc aagtgaactt
      541 cctgtcgaaa cagcgaatga tttggaactg tatgtcaata aagacatggc caaagcatta
      601 ggcatcaatc cagacagcat caaagaagct gaataaaaca caggaggatt ctcatgacaa
      661 atttgctctc tcttttctta tcatctgcat cgcaaggggt cttatgggcc ctacttgcga
      721 ttggtgtata cgtcactttc cgtatcttgg acattgccga cttgactgct gaaggaagct
      781 ttcctttagg tgcagggatc gcggcagtaa gtattacaaa tggctatcat ccattgacag
      841 cttgtcttct aggcttttta gggagtgcgg cagcagggct tgtttccgga ctgcttcaca
      901 caaaattgaa aattcccgcc cttcttgcag ggatcatcac catgactggt ttatattctg
      961 tcacatcccg tgtgatgggt gcgccgaaca tctctttatt gggtcagcca acgatctttc
     1021 attgggtcga gtcattcggc ataacaaaag acaacgctgt actgatcgtc ggtctgcttt
     1081 ttgctttgat cgttgtatgt ttacttgttt tgttcttacg tacagaaaca ggacttgcta
     1141 ttcgtgcaac tggcgataat ctggcaatga gcgaagcaaa cgggatcaac acagataata
     1201 tgaagatcat cggttatatg atttctaatg gatgcattgc tctttctggc tccttgcttg
     1261 cgcaaaacaa cggctttgct gatttaaatt ctggtattgg aacgatcgta attggacttg
     1321 cttctattat catcgcagaa gttttgttac gtaaccagcc actatttctc cgtctcttga
     1381 ctgtcgtttt aggtgcggtc atttatcgtt ttattttagc gttagtcttc gaactgaatg
     1441 ttgaaccaag tgattcgaaa cttgcttctg ctttcgtgct agtgatctgt ctttctttcc
     1501 cgcagatcca gcaaaaattc catttacctc aaaaaaagaa aggcggtagt gtcaaatgaa
     1561 tgacgtctta acattggaaa atctccatca tacctttgaa aaaggaacga tcaacgaaaa
     1621 tcatgtcctg aaaggcatta atctttcctt agatcccggc gacttcgtga cgatcatcgg
     1681 tggaaatggg gctggtaaat ctactttgct gaacagcatc gccggtacat tttctgtcga
     1741 tcagggtcaa attttattga atggaaaaaa tatcacgaaa aaatctgttg tcgaacgttc
     1801 gaaatccatc agtcgcgtat tccaagatcc taaactaggg acagccgttc gtttaacggt
     1861 agaagaaaat cttgctcttg ctatgaaacg aggaaaaaaa cgcggcttct tccgtggtgt
     1921 caaaccacaa gaccgaacat ttttcaaaga ccagctggtt cggctaaatt tggggttgga
     1981 aaatcgttta tcctcagaaa tcggtttgct gtctggagga caacgtcaag ctattacgtt
     2041 attgatggcc acattacagc gtccagaact gatcttatta gatgaacaca ctgcagcgct
     2101 tgaccctgct acttccaaaa cggtgatgga attgacagaa aagctgatca ctgagcagaa
     2161 tctcactgct tttatggtca ctcatgacat ggaagacgcg atccgctatg ggagtcgact
     2221 gatcatgctg catcaaggaa ggatcgtcgt agatatccag caagaggaaa aacagcagct
     2281 cacagtgccc caattattag cgttgttcaa acaaaacagc ggcacggcat taaaagacga
     2341 tcaagtactt ttatcttcat aatcagaaag aggatgggac aaaaaatgaa cgcccaccct
     2401 cttctttttt ctctttcctt ctttcgtttt tgctttatgt atcaaccgtt gtttcttcga
     2461 aaactatttt gttctctcct tcttcctgat agaatggatg ggaaaggagc ttgatgccat
     2521 gacaacgaaa gacaaggtac ttcaattatt gaaaaagaca ccagattttc tttccggaga
     2581 aaagttggca cagcaactgg aagtttcccg gacaagtatt tggaaagcca tcaaagaatt
     2641 ggaaaaggca ggctatcgtt ttgagcacgg gcctacaggg tatcgttacc tgccctctga
     2701 cgtactagat gcaaaagaaa tccagcaaga gctacggtcg tttattcctg atttgaaagt
     2761 acaaacgatg ttgacttctg aatctacgat gaaagatgcc aaactggcag cagctcaagg
     2821 agattctgtt tcgaagttat ttgttgctga tacacaagaa caagctcgtg gacgttttgg
     2881 acggcctttt ttcgctgaac ctggacgagg aatctacatg agtctcttgc tccatccaga
     2941 caaacggttt gatgaattgc ctcaatatac gatcatcgca gccagtgcct gtgtgaaagc
     3001 cattgaatct gtaaccggtc gttctgcttc gatcaaatgg gtcaatgata tctacatgga
     3061 tggaaaaaaa gtctgtggga ttttatctga agcagtcagt gatatggaaa gcggcaggat
     3121 cagtcatatc atcattggga tcgggcttaa tttttctgtt cctcaaaatc attttccagc
     3181 tgatctccaa gaaaaagcca tctctgtctt tcccgacgga caagctagtg tcacaaggaa
     3241 tcagctaatc agcaaaatct ggcaagaatt gttcacttat ctttctcacc cagaaaaagc
     3301 gttggacttc tataaagaaa aatcattcgt attgggaaaa accgtgtcgt tcaatcaaaa
     3361 aggaacgact tatgtcggtc ttgcaaaaga aatcggtgat catggcgaac tgatcgtcca
     3421 aacaaatgaa cagaagatga gtttgtcttc tggcgaaatc agtctcacat ctatccgtac
     3481 gcaataatta tcagatttca caaaaggaca cctgatctca tacagtgtcc ttttttctga
     3541 attctcttca aaagactctc cttattctat cagctgacag tttctctatc ctctagaaaa
     3601 aggtattcta aaaatatcta taaagcatgg tacactttct ttaaatgaat caatgcaaaa
     3661 aacaagagag tcatcagttt acacgatcaa aagagatagc aggccgcttc ctttttcaat
     3721 cttatagata gaccatgggt aacgataaaa acaaaaaaga acttgcgact tattttgccg
     3781 taagttcttt tttagctagt gccttaatct cttaatccca acaaacgtct cgcatagatc
     3841 acctgaccag agtgcatagc agcatcatca ataatagaaa cgagacgcat tcctcgtgtg
     3901 actggtggtg tccagttctc gtcaattact tcatctagct cggaaggttc cagccttctt
     3961 aagtaagaaa tagcaaaatc agcagctcct cgcaagtatc ctagcagatc ttctatttga
     4021 ttagcttgga tctgctttgc ttgttccaga ctatgcttcc aatcttgttc gttatctgct
     4081 atcgtcatcg gaaaccggct ttcccagtct tgactatgcc aaattggaga agttcctgct
     4141 aaatcagcaa tctggaaatc taattctcga gcggtatgcc aagccagcca agtcattgat
     4201 ttgatctgcg gggctctatc ttccactgga aaacgattgg cctcctcaat agagacgcct
     4261 tcaaacaaac gttcaaaccg atcgtatgcc cgatccacac tctcgcatag catatctaaa
     4321 taatccattt attttcctcc tttttattta tcctacttac tttttcctaa ttctaggata
     4381 cctacttata tgttactaca cacccttatt ttttacactt taacagtcac catcatcaat
     4441 agtttggaga ttgaaagaaa aagatagtgt cgaactatca attgaacaag aagcaacaat
     4501 atgaacgttt cattaatatt ctttaaataa acaatgattt ttcaaacgaa cagtttaaga
     4561 ctaggagaaa aagagtataa taggaaaatg tttgaaagga aaggaagggg aaaaatgaaa
     4621 aaaagggcaa gtttactagt cctagttgct ttgttgttca atatcttttc gtatggtgtg
     4681 gccgtttcag cggaaacata cattcctaaa gaccctggga atatttataa tgatctaact
     4741 ggtaactatg gcttgttagg aattgctagt caattccacg tatttgctaa aaataagaca
     4801 actgtgaatg cacatacaga cggaaatata gcaacacatg aacttgatgc aaaaaataat
     4861 tttggtacag agcttcatga aggtttattg aaccaagaaa taaattatat acaaaaaact
     4921 gattctttcg ctaattcttc aggcatacct acaggtagca caagaatgaa taaatttgtc
     4981 gttggcagta acgtggatgt agcacttcaa gatggtcatc ctacagtcaa tggatccagg
     5041 cttgatcact taactccaga tgatttttat aaagatcgtg ctgggcatga atatattgat
     5101 ttcgagcagg aatttgctaa attgaatgta gcagcaaatg atcttgcaac gatcactcca
     5161 gctaaaacat atactgctgc agattttcca gacatgaaca atcggacaat cgatttaact
     5221 gatttatcag ataccgggtt tttattggta aatatcgatg cagaagtgtt aacgatgaat
     5281 acgccacttc aaatcattaa tccaaatgat caagtagttg tatttaatgt catcaacagt
     5341 tcttctgcat tgaacgtata gtcaccaatc aaatacaatg atcgttcaaa tcatgaaaca
     5401 gaagattttt cggatgcgaa tatctcttgg aattttggaa atgagatgac tgatttgaca
     5461 atcagtgctc cttttcaagg aacaatcctt gcgcctaatg ctactatccg cattactcaa
     5521 aaccaagacg gaacgattat aggaacaaat gtgattttag atgcagctac caatcgttgg
     5581 gatcctaacg aaatatttat cagcgacaat ggtacagata ctacagacac aagcgacagt
     5641 acagatacat ctaccacaag tgatagctca gattcatcaa cttcaagcga cagtacagat
     5701 actacctcta ccacaagtga tagcacggat acatcagctt caagcgacag tacagatact
     5761 acctctacca caagtgatag cacggacaca tcagcttcaa gtgacagtac agatactacc
     5821 tctaccacaa gtgatagcac ggacacgtca gcttcaagcg acagtacgga tacatctacc
     5881 acaacaagtg atagcacgga cacgtcagct tcaagtgaca gtacagatac tacctctacc
     5941 acaagtgata gctcagattc atctaccact acaagtgata gcacggacac gtcagcttca
     6001 agtgacagta cagatacatc ctctaccaca agtgatagct cagattcatc aacttcaagc
     6061 gatagtacag acacgtcgtc taccacaagc gatacacgtc atgcaagtgt ctccttctca
     6121 aaaacaagtt cggataacag gacgaacttt aacttaacta ataaaacaag caatgacaat
     6181 ggaaacaaat caaaaagagc acttcctaaa actggaagtc aatcgaataa ttggataaca
     6241 ctcgcaggcg ttatattact tgtaatcggt ttaagaatca cttttatgag ttataagaaa
     6301 tcgaaaaggt aaaaaaatat tcaagataaa aaagagcaac gtcgctaacg gacgttgctc
     6361 tttatttttg ctttttatca taaatacaag aactacaatt caatttgtca aaaaacacgt
     6421 agatttcctt ctgagaaatt tatttacgta tcgaaccttt ccctattcag tctttgcgat
     6481 ccaaaatcag ttgggtaagg gaaaagagat tctcttttgt tcgaagagag gtggcaggta
     6541 atttctttat ttccttcaaa gccttttctg tgtaagaggc cgctaacttc ctagcttctt
     6601 ccacgcctcc tgtatgttca acgattttgc gtacggcgtc gacatcttct tctgtcattg
     6661 ccgctttctt ctctaaataa ggtagtagct ttggtttgtt tttttgcaaa gagtagatca
     6721 acggtaaaga atagacccct tgacgcatgt cttctagtac aggtttgccg atttcctggc
     6781 tctcttttgt ataatctaaa atatcatcga taatctgaaa ggccatcccg atagaaagtc
     6841 cgatatctcc tgcttttttt gcgaagcgct cgcttgttcc actttcaaaa gcgccaacag
     6901 aacaacttaa ttggaacagt tcagctgtct tacctgaaat attttccaaa tattctttta
     6961 cagaaagttc ataattatag cgatcatcca tctgacctaa ttcgccattc aatacttttt
     7021 ccatactgcg agaattcaat tggagacttt tcaaggaagt ggagtaatca gacaatagtt
     7081 taaaacagca gacaaataaa tagtctcctg catagacaga aacgacatta ccaaactgac
     7141 tacggatatt aggctgacca cgtcgcagat ccgcttcatc aacgatatca tcatggatca
     7201 gtgtggccgt atggagcatt tcgatagaag ctgccaaagc gatggctttg tctcgatctt
     7261 gctctggtcc aaattgtgag aacaatagct gataggcagg acgcaacagc tttccgccgg
     7321 catggatcgt ttctttgatt gcttgttcga caggtttgtt tttcaggcgg acagtttctt
     7381 ccatccgttt tagtgtttct gtcaattctt tacttaaagc aggatagttt tcccacattg
     7441 gatgtagctt cataaaaaaa ctccttgtgt gacgattatt ttttggtaaa cattgggctc
     7501 catttttgtt tggttccctg ctcataatct ttttggaatc gttgaagtgt ctgcacatgt
     7561 cgtaaaagat aattcgctgc gatcccgatt gcaatccctg ataaaatccc taaaactgaa
     7621 aggactggca agtacaacat gactgtccat gattgtgcaa tgaaagaagc catcaaaagc
     7681 tgtccgacat tatgcatgaa accgccggcc gcactaattc cgatgatact gacacgtttt
     7741 ggtcctaatt gcttgaccaa taacattcca aaataactaa gcaaagcacc actcatacta
     7801 taaaggaatg tggaaatcgt tccgcctagc aaagtagtca ggaatagtcg tagccaaagt
     7861 agtaagaagc tgtctcgttt tttcatggta aaaatggcaa cgatcgtgat tagattggct
     7921 aagcctagtt ttgcaccagg cgcaaaagaa aaaggataag ggatcatatt ttccaataaa
     7981 ccaatgatca ctccttgtgc aacaagtagc gaaatataaa tcattttttg taatttactc
     8041 atggtgtgtg aaggtctaat aaatcagact gtcttcgtca cctccatctg ttgaacggac
     8101 ttcgatcact aatttatgtg gcaaacagac gatggtttcg ccatttttcg ttgcccagcc
     8161 tcgacgtacg cagatctggt catcgcaatc tgcttctttt atccgtactc gatcgccatc
     8221 tatctcgatc aagttataat ctccgtgttc atctttgtaa gtatatgtgt tgctctcttc
     8281 tccagccatc aaagggaatg ttttgatttc tgtgccatcc acacgcagta ccgcttcttt
     8341 ttctgctgtt gcggtgtttt gctgaagacc aaaaatcatg actggtaaaa aagaaagaac
     8401 agtcaaaagc aaaataataa aaacatccca aggtttgagc agactttttt cagcaaattc
     8461 tttaaggctc atttttgttc tcctttttcc agattaactc acccaatttt agcataaaaa
     8521 caagaggttg tgacacctgt gtcttgcttt gagtaataag aacgaagaat agaaaacagc
     8581 tttccatgtt ttctaattct ttgggcttat taccgagaag cagcaggtgg aacaccgttt
     8641 ataacaagag gttgtgacag aagtgggcaa cttcaagaaa taagaaggaa tttacgaaaa
     8701 ttacttttca agtttttgtg aaatttcggc ttatttttcg aagaagctac ttctggaaca
     8761 ccgtttataa caagaggttg taacagaggt tgtgacacct gtgtcttgct catggaggct
     8821 tatctcgggc atcttattcc tttaatttta cataaaaagg aggctgtgac ggtttgtctc
     8881 agcccctttc tattgaaatc ttgcattcaa ataacgttct ttatctattt atttcttgcc
     8941 gtactgggaa cttggtctac cgtaccacca attacctagt gtacgttgta gccatggaat
     9001 tacccagcga tccaacccaa tcgcacgtcc ggaaccgttc atcaatgcga aagcaacaaa
     9061 aatgaaccaa atgtttaccc agtaaaacat gcctgataag cagaacgtga tcaccaatac
     9121 aatcgttgtc gcactgcata gccatgtgaa taatccagca atcaatgcta atgccaaggc
     9181 aatttcaaag aataccatga atttttggaa gaacattgct acatcattgt ttggcatgat
     9241 cacttgcatg atctttgtga accagtctgg tgacttctca atcacagcca ttggttcttc
     9301 accgtatgca taactcaaac caaaatgtgc agcttgagtc gctgcttctc cggcacttgc
     9361 ccctgctccg ctggcagctg tggttgcatc accagcagca gaagcaccac tggcaacatc
     9421 tgttacattg tattcccatg tccaagggaa cacactgttt ccagtgaacc atgatccttc
     9481 accaaaccaa gtacttggtt tgaacacttc accattccca atgatttttt tggaagcttc
     9541 aacgaaccaa actaaaccat agaaaatacg aagtggcaca ctccataaga cattgccgta
     9601 acgagaagaa tgtcctcgag tcacgttgcg atcatctttg atatggaaaa actcatgcat
     9661 taaatattgg aacatataat aaccagaacg aatatcaaag aagtatttca aattcacgat
     9721 atgtttcatt acgatagcta aaaatccgct taaatggatc ttatcaaata ggttagctac
     9781 cccccatttg gcaccaatag aaaccataaa tccttgatag tttcctttaa atttatgctt
     9841 ttctccgcct ttgatcgaag ccacgatatt cgctgcggct gtgtgtcctg tttgttcagc
     9901 agcttgaacg atctgcggtg tcggtgttcc tgggaattct tcataaaaga ctaaatcacc
     9961 aatgatatag atgtttttat cttcgtagcc ttttgcctgc atatattcat tggcgatcaa
    10021 ccgctgtccg cgtgctgatt ctaatccaaa gtcagcagcg tcagaagttc ctttgacacc
    10081 agctgtccaa atcagtgtgt gagtcggaat gttgcgtccg tctttcaatt tgatatgatc
    10141 agcagccact tctacgattg gtgcattcaa taataattcc acgttgtgct tacgcaaata
    10201 gcgttcagct tttgcagcat catttcgatc caacatattc aagatcgtcg gcattgcctc
    10261 tacgaccatc agtgtgattt cgtttggatc taatttataa tctttggcta ggcgattttt
    10321 ccagtcgatc agttccccaa tcatttcaat accggtaaat ccagaaccac agacaacgaa
    10381 agtcagcatg gctttacgca gttcatcatc tggttccaat gcagcttttg ctactgtctt
    10441 ctcaatatgt tcccgaatcc ttacagaatc ttcaaatgac caaagggtga atccgtgtgc
    10501 tttgacacca ggtgtaccaa agtcattcgg ctctccgccc attccaagga tcagctgatc
    10561 aaaaggatag gaacctaatt tggtcttgac gatttttcgt tctttatcga taccgattac
    10621 agtgtctgtg acaagttgta cattcttttg acgagcaaac aaatgctgca gatcgtattg
    10681 gattgcttct ggttctacac gtccgccagc tacttcatgc aactcggtca tcatagtatg
    10741 gtacgaatga cgatcgatca gagtaattgc tacatcttct tttttcagtt tctttgccaa
    10801 gtattttgta gctgaaacac cggcatatcc tgctcctaca acgacaatat tttgctttgc
    10861 cattgatcat ccgctcctta tagactatct atataatgaa tatatgataa ttcttttagt
    10921 ttgatactta ttacacaatc tccatatatt atatagatgt tctgttcttt tgtctaactc
    10981 cttttggatt tttttcaaat tttaaaataa agcgtttttt cacgaaattt gctgaaagaa
    11041 aacgtttgaa atattacatg ggttatttat caaaaatagt tgacttatag gctaaaaaga
    11101 ttagaatgta taatgaatca caaagaaaaa ttgtggacta gaaatggaat ggtggggtat
    11161 taaaatgaag aaaaaacgtt ttgtaactgg atttgccgta ttagcatttt cagctctagt
    11221 gttaggcgct tgcggcgcag atgacaacgg aagctcaaac tcttcttctg aatcatctac
    11281 agcacaatct tcgactgcaa aaagcacaac agaatcatct gcaaaagttg tagctggcgg
    11341 agatttgcaa gacggcacat ataaattaga agaaaaaaat tattcaaatg gttaccgtgc
    11401 agtctttgaa atggttgtaa aagacggcaa aatcactgaa tcaaaatatg ataacgtcaa
    11461 tgaaaacggc gaatcaaaaa caaaagatgc agaatacaac aaaaacatgg aagcaaaatc
    11521 tggcacaaac ccagaaaaat acattcctga gttgaatgac caattcttaa aagcacaaag
    11581 cgcaagcggt gtggaagtcg taactggagc tactcactca tctgaaagct tccaaaacta
    11641 cgcacaacaa ttgatccaag ctgctcaagc tggtaacaca gatacaatcg aaatcgacaa
    11701 tggtgctgat ttgaaagacg gcacttacaa gcttgaagaa aagaactact ctaacggcta
    11761 ccgtgtgcaa ttcgaaatga cagttgctgg cggcaaagta actgaatcaa actttgatta
    11821 tatcgacaaa gacggtaaat caaaacaaga tgatacagaa tataatgaaa atatgaaagc
    11881 aaaaagcggt acagaaccaa aaacttacat ccctactctg aacgacgaat tcgtcaaagc
    11941 aatgggcgaa gaagatggct cacctgctaa tgtagaagtt gttacaggtg ctacacacag
    12001 ctcacattca ttcatcatgt acgcacaaca attagtgaat gcagctgaaa aaggcgacac
    12061 tcaaacaatc gaagtagata atatcgtaac agaaaaataa tcacataatc atactaataa
    12121 aagttggagg gtgggacgat agccagatta ggcagtcgtc tcacctttca aaatgaagag
    12181 aggaaaaaga tgcggaaaaa actactattt tccttaatca cactgggcat ctcttttatg
    12241 gtgctaactg gctgcagcaa ctcagcaaat aaaacaacca ctgaaagttc agcagctacc
    12301 aagatattga aagatccata ttctgatgaa cagttcttgt tagggactta cgttcgtatc
    12361 cgtgtgtatg atgaaggcaa agaatcagca ttgaaacctg ccttcgatcg agtaaaagaa
    12421 cttggtgata aaatcacgat caatcaaaaa ggttctgaga tcgatgaagt gaatgagcaa
    12481 gctgggatca aaccagtaaa agtttctgat gacgtctaca cgttagtcaa gcgggcctac
    12541 gaatatagcc aagattctca aggcggattc gatatggcga ttggtgcaat cacacaatta
    12601 tggcgaatcg gttttgatga cgccagaaaa ccttcccaag aagaaatcga tcaggcattg
    12661 aaattagtgg attatcataa gatcgaacta aatgacaaag aaaaaactgt ttatctaaaa
    12721 gaaaaaggaa tgatcatcga tttaggtgca atcgccaaag gttacattac ggacgaagta
    12781 gtgaaagtct tgaaaaaaca aggagtgacc actgccatcg ttgacttggg cggtaatgtc
    12841 tatgtcttag gccacagccc aagaggcgaa aaccaagact ggacagtcgg catccaagat
    12901 ccgaacatgg ctcgcggatc tgttttaggt tctattaaag aacggaacaa aacattagtt
    12961 acctctggaa tttacgagcg ttatttagaa gtagatggca aaaaatacca tcatttgttt
    13021 gatccaaaaa cgggttatcc gttcgacaat gatatcgcaa gtgtcacgat cataacagat
    13081 aaatcaattg atggtgacgg gttgtctaca gcagtctttt caatgggtgt gaaaaaagga
    13141 ctggaatacg tcgaaagcga attgaataac gggacggaag cgatcttcgt gacaaaagac
    13201 gataaagtat atgtgaccga tccaatcaaa gacaccttca aattatcgga ggactcccat
    13261 tacacgatgg gtgaccgttc tgaactaaaa taaggagtac gaaccatgac actaaaaatc
    13321 tttttagaag tagttgaaat acgaacaaag gtggctagtg tatttccttt tgcggtaggg
    13381 gtattattct ctatcgcttt ttttcatgag atacattggc taaacacttt tttatttttt
    13441 attggaatgc tagtttttga tttagcgact accgctatca ataattacat ggattatgaa
    13501 aaggctcatt ctgaagtata taagtatgaa gaaaatgtca ttggccgcag aaatctatct
    13561 cctgtattga ttcgaaacat gattttagct atgattgcat ttgtttttgt tatcggcgtt
    13621 attctaacga tcaatacggg ttggctttta cttttgatgg gatttatctg ttgttttatt
    13681 ggagtattct atacattcgg accgattcct ttatctcgaa tgcctttagg ggaaattttc
    13741 agtggggtga ctatgggcct tggaattttt gccatggtcg tttatgtcaa tacattcggt
    13801 tcagatgtct atgatctgac agtcaacttt tcatctggac aatttgcttt ggtaggaaat
    13861 ctttgggcta tagcagcaat cgtattagct tccttaccct tggtgtttac gattgccaat
    13921 atcatgttgg ctaataattt gcgggatctt gaccgtgata ttgaaaatca tcgctataca
    13981 ctagtttttt atatcggacg tcctgcggga acagtcttat tccagctatt gatgtatgct
    14041 tgctatttcg tgattttaat ggggttgttg agcggtatct atgaatggcc gatcttggtc
    14101 accttcttga ccttgccaat tgtgaagaag catttggatc aattcaagca aacgttaccc
    14161 cagcctatca gtttcagcta ttccatcaaa aatatggtct tattcaatgc cagttatgta
    14221 ttggggctat tactaacaat cattatgcaa gccatttaac agcatgaaga ggttgtgaaa
    14281 aaactgccct gcatcaagta ataagaacag ccaaataaaa atagctcctt atatttttcg
    14341 tttggttggg cttattaccg cagatgcaag cttttgaaca ccgtttatca ggaggttgtg
    14401 aaaaagtcgc ttagtttcga gtattaagaa aaattttgtg aaaatggctt tccacatttt
    14461 tgcataaatt tggcttaata ccgagaaact ggcttttgaa caccgtttat cagaggttgt
    14521 aaaatcagtg ttttgtcctg agtattagag aaacaaatgg aaaaatagtt cctcatattt
    14581 tgccgttttt tgagctaata ccgaaggaca gctgattgaa cactatttgt tcggaaaaag
    14641 tgactaacta tgacacaatt tttttcacag tccctttttt cacttgattt gattcgtacc
    14701 agcgaacttg acgagaactt tcttggtttg gtaactatat ggtattcttt taacatcctc
    14761 gtttgcttct tctaatttat ttatacaaag tcacactttt ttaaacatct gttcttcgat
    14821 acatttctat ttcttttaaa catcgatacc aatattttga acttaagagt gcataccaaa
    14881 tggattcatt tactcttcgt cagttacgaa gataatcaaa atctccaact tccctactta
    14941 tttcgttttg agaattatcg aagcaatctt acataaataa ctaaaaaaca aaaaaccaga
    15001 aaatcagtat actgattttc tggttttaca tggtttgttc aataaaaagc tgttggaact
    15061 gttaatatta aattatcgca tcgttacgaa ttcttccgct cctgttggat ggatggctac
    15121 tgtattgtca aagtcttctt tagttgcacc catcttgata gccacggcaa aaccttgcag
    15181 catctcatcg acaccaacac caatagcatg taagccgacg attctttctt cttttccgac
    15241 acagatcaat ttcatttcgc atttttggcg ataatcattt aatgcaaagt acattggggt
    15301 aaagcgtgaa cgataaactt tcaattcatt ttcgccatat ttttccaatg cttcttcttc
    15361 tgtcaaacca attgttgcaa ttggtggatg cgtaaagacg actgtcggaa caagtttata
    15421 gtcaagataa gaatctttct ttccattaaa taaacgttct gataaacgtc taccagctgc
    15481 gattgcaaca ggagtcagat ctaatttacc gatcacatca ccaacagcaa aaatatgttc
    15541 atccgttgta ttttggtatt tatctacttt gataaaccct tttttgtcca attccacatt
    15601 tgtattttcc agacctaatt tttcggtatt cggtacacgt ccaccggcaa aaagtacaca
    15661 atcaccagtt aaggtttcac cattttcaaa tagcaccgta tattcgtcat tgtttttagt
    15721 aatttcttta gcaatatgat gtgcgtaagt ttttattcca gcttcttggt acatttggat
    15781 caaattgtcc gaaagcattt tatcaaacgt tctaagtggt cgttcttttc gatatgccca
    15841 catgacttca ctacctaaac cacaaaatac acctgaaagt tcagccgcaa tgtatccagc
    15901 acctaaaacg attgctctct taggtaactc tgttaattca aaaaatccat ttgaatcaat
    15961 tgcgtattct ccaccaggaa tggtcatttt acttgcacgg cctccagtag cgatcaagat
    16021 tttaggagca cgataggttt caccgtttac ttcgatcgtt tgactatcga taaaagtagc
    16081 gtaacctttt atagcctcga catgattgct atctaatcct ctttgataag caccgtgtag
    16141 aaaatcaata tatttttctc gtctttctac tagaccggcg aaatcaacat tttttaaatc
    16201 tgcttggata ccgtaacttg aggcatctcg ttcaattgtt tccaaaatcg tacttgcttg
    16261 ccacataact tttttaggta cacaaccgac gttgacacat gttccgccca attcattcgc
    16321 ttcaatcagc aaaacttttg cgccatgcat ccctgctctg tttgctgaag caataccgcc
    16381 agagccgcca ccaattacga tgtaatcata tatttgcatt ttgttcctcc ttattgaaga
    16441 gatcaatcaa aacctttatt taacgatcct ttttctacag aaaacaagtg taacactttt
    16501 cttgaatttt gtatgcgaag atgcttaaaa cagggatata tcagcttttt ccttatttac
    16561 gttttcggat catatctcca ggtttcaccg gctgtgcgac tggcatagtt aaaatcatca
    16621 ttggatttgg tgctcggtca atggattcgc cttcttcatt atgcataact tcaacggttt
    16681 gactgaaatg actgaatcct ggaccataaa attcgatttc atctcctaca gaaaacaagt
    16741 ttctctgacg tatcgtagca atctgtgttg cctcatcata agataatact tcaccgacaa
    16801 atttatatac tgggatttta cgacgttcac cgaataattg ttcgttttca gttggtgtat
    16861 tgtagtaaaa acctgtagct aattctcgtt gtgctacttt ccataactcg tcgatccatt
    16921 cttgcttgca gacatagttt tcaggatctt ccatataact gtctacagcc gctttataga
    16981 catttgacac cgtagataca taatggatcg atttcattct tccttcgatc ttgaagctgt
    17041 ctacgccgtt ttcaatcaat tctggaatat gttcgatcat tgacatatcc actgcactca
    17101 tcgagaattc ttcttccact tcgcctgtat cagttaaact gtttcgttct tgaccaaaag
    17161 gcatatcgaa caatccatac ttccagcgac aagattgaga acatccgcca cggtttgcat
    17221 cccgcatcga catatggttg gacaatgtgc atcgaccaga ataagagata cacatggcac
    17281 catggataaa tgcttcgatt tcggtatccg tatttttacg aatctctgcc acttcctcca
    17341 ttgaaacttc acgagctaaa acgacacgtt ccaacccttc ttctttccag aattccaatg
    17401 tctcaaaatt agttgctgaa gcttgagtgg ataaatggat cggcagacct ggggcttctg
    17461 tcgcacaaat ttcgatcaaa gctggatcag aaacaatcac tgcagaaata ccgacatcac
    17521 gcaaactgcg gaaaaattcc cctgctccat cttcatttcc ttcatgggtg accatattag
    17581 cagcaacgta taccttcgca tcatgttctt ttgcaaaagc aactccttca gccatttctt
    17641 cataagtaaa attacctgca cggcttctta aaccatatgc attcccgcca atatatactg
    17701 catctgctcc ataatggatc gcggttttta acttttccag tgttcctgct ggcgctagga
    17761 tttctggtcg tttcaatttt ctttttactg tcatttcttt catcatactc cttatttgat
    17821 agcagaagga tcaatataaa agaatcctgt atctaattca cggttttcag gatgcaattt
    17881 actgatttcg ctagtcattt tttccgcttt ttcttgatcc cagctacctt ctaaaatttg
    17941 tttttttgct tcggcgaata gtcgagtgat gtttacgaat ttttcgcctg gagtaaataa
    18001 tccgtccaat ttccatgtac agtaatgatg ctgataaagt tcattcaatt cattcgagag
    18061 attcagatca ttgctggcaa aaatatgtgt gccatgacta tcttcataaa tagaataatg
    18121 tgtctcttct tttttaggtt ccgagataaa aagacctctt tcacgatctt tttgttcgtc
    18181 ttgtttcgta tagttatagt aattctgtag cagtggacgt ttggattgat ggatgcatgt
    18241 agcaccatac acaagggttt ctacaggaat atcgagtttt tcttccatcg ccttcatctc
    18301 ttcaaaagga acttcacgag caagtactgc tccaactgcg ccttttttcg cccagaaatt
    18361 gatttgacgt gcgctcgtta ctaatgtttc agcatcataa atatacggta tacgcaattc
    18421 aggatttttt ttcatcgtat aaataatacc tgtatcacca actgtgatca catctacgtt
    18481 tatttctttt aaaaaagcca aatattcagg tacatttttc attttttcag gatgaatgat
    18541 cccatttaca gcaatattcg cttttttacc atacttgtgt gctaattcta ccaattgtcg
    18601 ctgttcttct cgtgtaaatg aagcaggcag acgcaatcca aattgctctt caccgaaata
    18661 tagtgtatct gcacctgcag caagcaactg ttctgcttgt tcaatagttt ctactgtggt
    18721 tatgagttcg atcataattt tctcctccta caacttcata atagtagcat agctctaaaa
    18781 actttccaat acttcttcta tttaaagtcg gatttttgaa aattatttca caaaaaatca
    18841 aaataaagtt cgatatattc tgtccaaata taaaaagaga ttgtgacaaa aatcttgtca
    18901 caacctctta atcaagtctt actattataa aaattcagct acacaggcca agtaactata
    18961 gggaacctac tgtttccttt ttaggtccgc gaacattcaa tgtgatctta ccttttgaag
    19021 cacctactgt tacgtggtca cctaaacgaa tttctccgcc aagcaaagcc tcgctcaatc
    19081 gatcttcaat ttctttttga agtgcgcgac ggattggacg tgcaccatat tcaggatcaa
    19141 atccagcttt cccgatcaca tcgattgcag atggtgtgat tttcacttga atatcttgtt
    19201 ctttcaatcg tttgataatc gctttgctca tgatttggac gattgtatgg atttcatctt
    19261 tactcaatga atggaagacg acagtctcat cgattcgatt caagaattca ggtcggaaag
    19321 ctttctttaa ctcttctaag atacgttttt gcatggcttt gtgatcttta gtaacatctt
    19381 ggacattgaa tccgacattt ttttcttctc gaatttgtgt cgctccgata tttgaagtca
    19441 taatcatgat tgtatttcta aagtccactt ttctaccttt agaatctgtt aaatgaccat
    19501 catccaatac ttgcagaagt aaattgaaaa cgtctggatg tgctttctcc acttcatcta
    19561 agagaatcac tgaatatggt ttttgacgaa ttttttctgt caactgtcca ccttcatcat
    19621 accctacgta tcctggagga gacccgatta atcggcttgt actgtatttt tccataaatt
    19681 ctgacatatc aacacggatc agggcatctt cacttccaaa catgacttct gaaagtgctt
    19741 tggccaattc tgtttttccg acaccagttg gtccaaggaa catgaatgaa ccaattggac
    19801 gatcaggatc tttcaatccg cttcgtgcac ggcggatcgc acgtgaaact gctttgacag
    19861 cctcatcttg accaacgaca cgttcatgca gaagtccttc cagttctaac aagcgttcgc
    19921 tttctttttt ctccaactgc tgtagaggga caccagtcca ctccgataca actgtagcaa
    19981 catcttcttc tgttacacga tcagaataac cttgctcctg ttttgcttca attgcagaaa
    20041 ctttttgcag ctttttacgt agtgcttttt cttgtctgcg taattgagca gcattttcaa
    20101 agtcttgttt ttggatcgct gcttcctttt cttgtactag ttcatcgatt tctagtttga
    20161 tcacagttga ttttgttaag tgatcggttt gatccaaacg gactttcgct gctgattcat
    20221 cgatcaaatc aattgcttta tcgggtagtt gacgagaagt gatataacga acagaaagat
    20281 tgactgctgc tcgtacagct tcatctgtga tctccactcc atggtgctct tcatatcttg
    20341 tacgtaatcc ttgaagaatc acttctgctt cttcaggagt tggctcatct acttgaatgc
    20401 gagcaaaacg tcgctctaat gcagaatctt tttcgatata tttttgatat tcatctaggg
    20461 tcgttgctcc gatcgtttgt aattctcctc gagctaacgc cggtttcaag atattcgaag
    20521 catcaattgc tccttctgct ccacctgcac caattaaggt atgcaattca tcaatgaata
    20581 agattacttg accatcatta tagatttcgt cgatgacctt tttcattcga tcttcaaatt
    20641 caccgcgata tttcgtacca gcaaccaaag atcccatatc aagcatcatc aggcgctttt
    20701 cttgcatgtc ttctggcact tcacctaaaa tgattttttg agctaaccct tcagcaatgg
    20761 ctgttttgcc tacaccaggt tcacccacca gtacagggtt attctttgtc cgtcggctaa
    20821 gaatctggat aagccgtttc acttctctcg aacgaccaac aactgggtcc agcttgtttt
    20881 cttttgctaa tttcgtcaaa tcacgtgcta atgaatctag cgtaggtgtt ccttgcggtg
    20941 tttgccgttt atcttgtaca tttacatttc tacgacgatt cattcctttt ccagcagaat
    21001 caccagctag ccccattttt tgtttcagaa cttgacgggc ttttgaaagg ctgataccta
    21061 agttgatcat gattcgagta gccatgatac tttcatcttt taataagctc atcaaaatat
    21121 gttctgttcc aactttttga gaaccagttt tcttcacttc gctgctagct aaagcaaaaa
    21181 cttgtttcat tcttggtgaa tatggaagaa agactccttt agggtaagaa cggataccac
    21241 cataaccaat cagatgttca atttcttcat aaatttccgt ttcattcaaa tccatttcac
    21301 gtaatacttt tccagcaatt ccattttgct cgaccactaa ggctagtaaa acatgttctg
    21361 aactaactga ttgatgtttc aaccgttttg cttcttcttg tgcaatttct aatactgctt
    21421 gggctctttc agtaaaaagt tcttccatct aatctcactt cccatcttca taacttaaac
    21481 gttccagaaa tgctcggaga atccgagcac gtgtatgatc ttcatattca ttgaaattca
    21541 atgtgttttt tgcaattgca ctcaacatca agtttccttc tttttttgta attatttggt
    21601 cttcataaag tttttgaata atagcaaaag catttttctc actgattgta tcatcaaata
    21661 actgatttat ctgctcaagt aattgttttt tatctgaaat ccttactttt gcaattcgta
    21721 tataaccacc accaccacgt ttgctttcta ctagataacc acgttggatg gtaaaacggg
    21781 tattaatcac atagttgatt tgtgaaggaa cacagttaaa gaggtctgcc atttcaatcc
    21841 ttcgaatctc gatcatttca ctattttcca gaattttctt tatatatgat tcaattaaat
    21901 ccgaggtatt ctgatgtgcc atcttattca ttccctttga ctaatattga ccttaattat
    21961 aacgaatttg ataatagaaa gaaagaagaa cgctttttgt taaataaatc ctttagaatc
    22021 aacaaaaaaa aatacggaat atactattcc gtatcgacgc gcccaagagg agtcgaaccc
    22081 ctaacctttt gatccgtagt caaacactct atccagttga gctatgggcg catattcaat
    22141 ttaaaaaaat tataaatcat ttgaaaacaa aaagcaagat aaaaaaaatc gggaagacag
    22201 gattcgaacc tgcgacccct tggtcccaaa ccaagtgctc taccaagctg agctacttcc
    22261 cgtttaaaaa ataaaaaaga c
//
LOCUS       DAZKKW010000043        22171 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.043, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000043 DAZKKW010000000
VERSION     DAZKKW010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22171
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.043"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(26..328)
                     /locus_tag="ACG8PO_001582"
     CDS             complement(26..328)
                     /locus_tag="ACG8PO_001582"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0206894.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            complement(353..2446)
                     /locus_tag="ACG8PO_001583"
     CDS             complement(353..2446)
                     /locus_tag="ACG8PO_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFQ0206895.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKGFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            complement(2935..3699)
                     /locus_tag="ACG8PO_001584"
     CDS             complement(2935..3699)
                     /locus_tag="ACG8PO_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HFQ0206896.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            complement(3810..4679)
                     /gene="mdcB"
                     /locus_tag="ACG8PO_001585"
     CDS             complement(3810..4679)
                     /gene="mdcB"
                     /locus_tag="ACG8PO_001585"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046917 - triphosphoribosyl-dephospho-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0019361 -
                     2'-(5''-triphosphoribosyl)-3'-dephospho-CoA biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HFQ0206897.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            complement(4704..5780)
                     /locus_tag="ACG8PO_001586"
     CDS             complement(4704..5780)
                     /locus_tag="ACG8PO_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFQ0206898.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            complement(5804..6397)
                     /locus_tag="ACG8PO_001587"
     CDS             complement(5804..6397)
                     /locus_tag="ACG8PO_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HFQ0206899.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASPDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            complement(6401..8077)
                     /gene="mdcD"
                     /locus_tag="ACG8PO_001588"
     CDS             complement(6401..8077)
                     /gene="mdcD"
                     /locus_tag="ACG8PO_001588"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HFQ0206900.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            complement(8067..8366)
                     /locus_tag="ACG8PO_001589"
     CDS             complement(8067..8366)
                     /locus_tag="ACG8PO_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HFQ0206901.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            complement(8468..10114)
                     /gene="mdcA"
                     /locus_tag="ACG8PO_001590"
     CDS             complement(8468..10114)
                     /gene="mdcA"
                     /locus_tag="ACG8PO_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015451 - decarboxylation-driven active
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HFQ0206902.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            complement(10121..11032)
                     /locus_tag="ACG8PO_001591"
     CDS             complement(10121..11032)
                     /locus_tag="ACG8PO_001591"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFQ0206903.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            11152..12009
                     /locus_tag="ACG8PO_001592"
     CDS             11152..12009
                     /locus_tag="ACG8PO_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFQ0206904.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            complement(12196..12558)
                     /locus_tag="ACG8PO_001593"
     CDS             complement(12196..12558)
                     /locus_tag="ACG8PO_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFQ0206905.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            complement(12747..12929)
                     /locus_tag="ACG8PO_001594"
     CDS             complement(12747..12929)
                     /locus_tag="ACG8PO_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0206906.1"
                     /translation="MIFFISEYINQTPFYSKNTEKINGGQVSTDFSIIVYFSGAFTDD
                     KNFIMNLKNDTTKIKK"
     gene            12904..14343
                     /locus_tag="ACG8PO_001595"
     CDS             12904..14343
                     /locus_tag="ACG8PO_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0206907.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            complement(14350..16902)
                     /locus_tag="ACG8PO_001596"
     CDS             complement(14350..16902)
                     /locus_tag="ACG8PO_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HFQ0206908.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMGFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(17016..18347)
                     /locus_tag="ACG8PO_001597"
     CDS             complement(17016..18347)
                     /locus_tag="ACG8PO_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFQ0206909.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            complement(18313..19791)
                     /locus_tag="ACG8PO_001598"
     CDS             complement(18313..19791)
                     /locus_tag="ACG8PO_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFQ0206910.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            complement(20096..21931)
                     /gene="tdc"
                     /locus_tag="ACG8PO_001599"
     CDS             complement(20096..21931)
                     /gene="tdc"
                     /locus_tag="ACG8PO_001599"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004837 - tyrosine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HFQ0206911.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
BASE COUNT         6484 a         4724 c         3788 g         7175 t
ORIGIN      
        1 tttttaccct cttttctttt tgtatttaga taactccctt atcttgcaga actggggtga
       61 ttttttcttc aatttcattt tttgaaacaa tatttttcaa atagatcatc gccgttttag
      121 gatcaactgc gaacttttcg aactgcgaac gaaaattttc tgctgtgatg atcacatctg
      181 gtttcatgga aactgcggaa gaaatatcac aatggtccag tttggcttct actcctaatt
      241 tgttcaaaac atcctcagca gacatctgtg ctgcaaaact acttcctaaa cctgcaccgc
      301 atacgaataa aatgttcaat ttgttcatct ttgttctccc ccgtatcatt tattatttct
      361 caactactga aaaaagcagt tgtttgaaat cttcgatatt tgtgcattca atcaatcgat
      421 ccaacttctc ttcgttgttg atcaattcaa ttagttcttt catgatgttc agatgcgagt
      481 aggagtctac tgctgccaaa caaaaaataa tctttactgg atccatttca ggattaccga
      541 aatcgattgg ttgctcaatc gtagctacgc tgactcctaa tttattgacc ccatcttctg
      601 gtcgggcatg ggctagtgcc atgtgtttgc caatcacgat atacggtccg taatcattca
      661 ctgcacgaat catcgcttcg acatagcgct cttcgacaat gttttcttta accaatggtc
      721 tagcagctag ttcaatcgat tcctcccaag tagtggcttt ttctttgatc atgatattgc
      781 tgtccgtcaa aatatctttt aacataggct ggatctccct tttgttgatt tctaaattgt
      841 ttgtttcgaa taatttttct aacttgccat agatgattcg atctactcgg ccaccacttt
      901 ctttcatgac atctattatt tgagtgaata actccgtcga ttcatgactg ttaggctcta
      961 accgctgtag ttcttcattt cttttcaaaa agtcttctat aaccaagata ttcgtctcag
     1021 ttaaaatcgg atcgacaact aaaagcggct tatttcgata attcaattga aaggtgctga
     1081 aaaccagatt gacatccaat ttatctacta attcgatttc ttttgaactt aaaactgcag
     1141 taatctcgat ctgaggaaac ttttctttca gattctctgc taacagattt ccagtagcca
     1201 tcccatggtt acagacaacg actgatttat aaacataatt caagtctcgc ttgattacac
     1261 tggcgatcgt cgaaaagtgg atgacaagaa aagctatctc gtcctccagt atcttgccac
     1321 caattacttc ttcgatcgta ggaacaaaat gagtgactgc gcggtagatt gggaaataat
     1381 ttgttcggat gttttctttc aatggattca tgacttggat atgattcttg attcttacaa
     1441 tcagtgcagc catgtgctta tagagatttt cgaataaagc ttcttccttt agatggaaag
     1501 gtacgtgtgt ctcgttctct acaaattgga tcaattggat cgttagtaat tgagcttgta
     1561 cccactcgat tgaattggag atatctctag cattgaacgt ctcaatagtg aaacgaagat
     1621 aatttctttc tacttgcggg acattgtcca attgaaattc ctcgatcatg tggtcaataa
     1681 atgcagaaaa gctactgtct tttatttctg tatttttcca attaatggca gagataaatt
     1741 catgtttcct gattcgctgg atccagatca atgtaaaaag caggatttgt tttttataaa
     1801 tatcatcttc ttgtttcaac aacttattgg aatagatctc ctgtattttt ttgatttcca
     1861 ccaaaggaaa ataatggaga aagatttttt gagccggtgt taattgtgca tcttgattca
     1921 taaagaagtc ggtcattccc agattctgat tgataaaatc atagatcatc gtacggatag
     1981 atcgttctga acctttataa accagtcctt gctttccata actgacgatc tctatcccat
     2041 aactgaccag tgttgctcgt aaccgtctca tatcttcatc taatgtactt ttagagattt
     2101 gaaattcttc ttcttttttg tttagatgga tcgggattcg gctgtaagca acggctaaaa
     2161 ctagatcaaa aatacgttcg cgccgattca aaaagccgtc tttcacatct tttatttcag
     2221 tttctaatcg agcttgcttt tccttttcca gcttcaacaa aaaacctttg ttgcgaattg
     2281 tacggatctc cggaagcttt ttactgctca accagatatt cgcttcaaga acttcctgac
     2341 ggatcgtccg ctgactgaca ttgaactcac ttgctagatg ttttgctttt actggatatt
     2401 gggctgtcat gattctgtga agtatttcaa ttgttcttgt tttcatagaa atgatctcct
     2461 tctgatttca agtatatggt tccgcttaca taataaaaag tagatatttc tgccacttga
     2521 agtggaaaga aagtaagcaa agcaaacaaa aaataagcca tatgcttttt catctcctca
     2581 acgaaaaaga catacgccta ttattacaaa aattttgccc atctatacaa aaaagaggtt
     2641 gtgaaaaagc tgtcctgcat caagtaataa gaacaactaa actaaaatag tgcttcctat
     2701 ttttcgtttg gttgttctta ttatcgcaga tgcaagcttt taaacaccat ttagtggaga
     2761 ggttgtgaca gagatgttca gccttgagcg ataagaagcg attttgaaaa atcgcttccc
     2821 atattttaca aaatcgtgcc ttattgccga taggctatct ctggcacacc gtttattcag
     2881 aaaaaggagc tgcgacaagg cttttgtcac aactccgttc ataaatattt cctgtcaatc
     2941 cagcataatc ggctgaataa aatcggcttg ccatcttttt tctttctcag aaaaatcaaa
     3001 ataccatatc tgagaaaaat actgtcgttg tgtcttactt cctgagatca atcgcccatc
     3061 ttttcgatcg actgaataat cacagcatct gaaatcaatt cggatggcaa aactattttc
     3121 gtggatctgt cgataatttc ctacttcctg aatagttatt tttttagtat gatttcttac
     3181 accgtctgct ttgttttttt gcagaacttt ctgatgttcg gcaaacagct tgtctgtata
     3241 acagcttctg gctttttcag gagtttcttc ttcccaagcg gattggatct tcatgaatat
     3301 cttttggatt tccagaaact gtgctttctt ttctttagcc gttcctgaca tgcgggacaa
     3361 tatctccgct ttcagccttt tcttttgtct tttattcttg acgatagaaa aagcagaaac
     3421 ccctcctact gacaaaacag ctaatgcaag aaatcccgtt tgctcataac tagaaggacc
     3481 tgtacctgga ccataatacc cataagaatc tctgccgtaa gaacgactgc caaaataact
     3541 gcccccatat gaacgaccac caaatgaacc tctgcccgaa cttcgaccgc tagatgtacc
     3601 tccaccatga ccacccgcga cagcgaatcc ttgggtcggc agagcaaaca aacataggcc
     3661 cactaacaaa atgattgcta aataattgat ttttttcatt gtcttcctcg ttatctttta
     3721 ttacaaaaaa ctatttttat gatagccttg ctaaaaggaa gagtcaataa ccagatgcat
     3781 aaactgtttt tttagcaatt ttgtcttatt tagtcattga gtaacatcgg acaaatgtat
     3841 gtgtcggcag tccataattc ctctatcttc agcatgaaca agcttgcaga caagagatct
     3901 gcgcttcctc caggagagat ttggttttcc tgacaaaagt gcaacagtgc tgtaaatgga
     3961 cgatttggta aagcactttg actagcagca tgagcaagtt tttgcatttc cactaacgtt
     4021 ttctgatcac tgcgatgcaa tacgcatgtg tcatctagcg aactcatcaa aactaacaac
     4081 atatgcattt gtttctgtac atatgaatca ttatctagct ccttagctgc ttgaattgca
     4141 agcctgatat gcggataccc taatatcgct tcttcatatg caccatttac gccatatttt
     4201 tgcttcaccc tatgaccatg agtggttttt ttatgacgat attttgtatc aggaaaagca
     4261 gcaagcttgc tgacatcatc aagtattttc gataaagaaa aaggctgttc catactgatt
     4321 ttttgtgcaa tgacactgat caatagcccc attgcccaaa tcgctccttt atgggtattg
     4381 atgccatttg tagcaatcag cattgccttt tccgcttttc gcccaatctc agcaattctt
     4441 tctcttaact cctgatcaat agcatgatgc catgctattt gagccatttc ttcaaaaaaa
     4501 ggagttagcg tcttagcaga cttaagaaat aaagcatagt ccatatcgga atgtgcaccc
     4561 gcatccagtg catccactaa tccaggtttt ggacttaatg ttacttctgc gatcaatgct
     4621 tcctctacca acgcagcgag ttgctgagtc actgcttttt ttcgttcgtc atttatcatg
     4681 acactcactc cttcttccta ctattacttg ctgaaacctt ttttgatagc tacccaaatg
     4741 atatgaagta accagcttaa agcaaatgca caacttgccg tcattagata agctacaaga
     4801 atcatgactc ctgaaggtaa ctgcagccaa ctatctttaa acaataataa cagtacttgg
     4861 aaaacaaata cattggctaa atacgtacga taagcataaa cagacaacca tttgaacaat
     4921 ggcaattttg gcgaatcgtt tacaatcatt ttggaagcgc ctatgaagac agcaaagatg
     4981 atgactaaac tgtataaact ttggatcgta ttcaagtaag gtgcattcgc aaagctcaag
     5041 tctcctggat gtgcgagcaa tgaacggaca gaaaagaacg cgataactaa ggcaacaggt
     5101 aagaaaagat aacgtactct gtccaaatat tgatagactt tttcgtgata aacaaatgcg
     5161 gctaccccat aaatcatgaa actagcaaca aaccgatcta ataagtacca gctgtcatga
     5221 taaggcccag tcaatacttg tgtttgatag aacagatacc atgcgacata taaaatcgtt
     5281 gaaccaatca atactggcca tacatatttt ttattagcaa atactttgta accgagccaa
     5341 acaaaaaacg gcatgaacaa ttggatctgc agcatcataa cggtatacca taaatgaggc
     5401 gctccatcac ctgtgacata tttcagtaaa aagcttgcag tatccgtata cggagtttgt
     5461 tgttggactg ctggaaatag ccaaagataa gccgtcgtcc atagaagata cggaacgact
     5521 agttcaaaaa acttttcttt caaaaattcc caataagaca aatgctgact tgtttttacc
     5581 atattataaa caattgcaaa aataaacatt ggtgctgtgt atttaacgat gacgtaaaag
     5641 gtaccaagaa aagctgtatt tgttgcgctg tggctgtttg ccaaagcaaa gctcaatatc
     5701 gtttgcatca taacagccgt tacagctgat actttctgga agtcacttac gttgattttg
     5761 atttttttgt tttcaaacat ctctttctct ctctttttct ttattaccaa agttctttgg
     5821 ttaatttagg tccttcgact gttttcaata gaattttttt ctctggctct ctcaaatact
     5881 cttttagtga aaaaccgcct tgccgagtca ctacttgggt atcgaattgc tcaaaaaaag
     5941 caggatgcga ttgaattacc tgaattggca attcaatcgg tgaatttgcg tacagcagca
     6001 aatcaaaatc actggtttct tttactgccg gaatcccagt cgataattca aatcctaagc
     6061 tgccgccgat tccccattta catcctgata atatgtttct agcagcatga aattgctgat
     6121 aaacaggata atccatgatt ttctcttgtc tatacgattc ttgttcgatg atgtcccacg
     6181 gatgaatgac ttgtttgacg gcatgttttg agatttctgt tgaccatctc tgattgcggg
     6241 caggtccacg taaaccaatc ggtagtattt ctgcgtgatc cggcgacgca cgacgaactg
     6301 ttccgaacat cttttcagtt agaacgaccc attcaggcaa ggtgaaccct tctatttcat
     6361 gtttatcaaa aaggacaata tcatgtgctt gaatcatttt ttatgcatcc cattcttcgt
     6421 ccattttggc acggacttta tttgtttcga cacgtccgaa tgttttggct gtttcgtttg
     6481 tgtatcgatt tgtcagcatc gtgtcatttg tattgcggac atcttgaatg gcttcattga
     6541 ttgcttcgat gactttgctg acattttgtt ctgaagcagc ttggtcggtg atatcttcta
     6601 ggaaatgata gatcgctcct agttttgtat aattattgat atcgtaagcg atagctggaa
     6661 ctttttcagc agctgcttcg atttcagcaa ccgaacgttt tgtcacacgt gcagcacttt
     6721 ctttagacat cgcttggacg gtaatttctt tattgtttaa agcaatcaag cggcttgatt
     6781 gtaatccatg tgctaaaaat gcacctgaaa tagcatttcc tgcaatcaat ccaataactg
     6841 gatgtccagc ttgacggagt tttgcataag cagcagcact attagccaaa gccacatgga
     6901 taccgatcaa ttcttcgtta tagccgtatg cttggcttgg tacatcgatc acaacgatga
     6961 ttggccgttt cacttttttg tctttatctt cttcgtaaac gtgatccaaa atttgtccca
     7021 tgcggaaacc ttcaactaaa ccaacttcac cgtttcgtac acggtacatc gggtttttag
     7081 gatcaggtac gattgatgtc acgatggctt cttctatgaa tttacttgat ttaccataat
     7141 acacagtact aatagaagaa gttggatttt tcatgccagt tagtgccgca aaccaatcgt
     7201 aaccaacact tgctgcagct ggttcatttc cttctgctac ttctggtaag tcaattgatt
     7261 ttgcttgatg gttagcaatt gcttcattgt aatcgtcaat ggccattggt tttgaaagat
     7321 ctaaagaatc aagtaatgat aaataaaatg ctgagctttc actgcgatgc gtgtcttttc
     7381 gttcgtccat tgctgcgatg acagcagctt tgattgcttc tacactatca gtcactaatt
     7441 catcaatgat ttttgattct acccgttgac gagatcctaa tgtattccaa atcaagtctt
     7501 tgtcactaga atcaaattct cgaacgcctg cttcttgttc aataacttct ggtccattca
     7561 aaccgactct cgcttttttt gttgcgatta aataactcat taatgctgaa gtaatcgaca
     7621 taccgccaaa tgatcctaca cgtcctggaa tcaaaccgat gactggtaca tattgtttta
     7681 gagcaatgat tacatttccg atttctgaaa tagataacag tccgtagttt gcttcttgca
     7741 aacgaacacc acctgtatct agtacaatga ctggaaagat ctcatttcct tttttatttt
     7801 cttctaacac atgttcaagt gcagcaacga tttttgcacc ggaaacttca ccgataccgc
     7861 caccttggaa ttttccttcc atagcgatta ctactgcttg tttgccattg atcgtgccac
     7921 ggctgacaac gatcccgtca tcactttcag ggacgattcc ttgtgctggt aaacttggcg
     7981 cgatgatttg atcgaatgga tcaatcaatt cacgtccatt gtcaagtaga gcgttcactc
     8041 gttctctggc gtttaattct acaaaactat ttttcatctg aaagttcctc cattgcttgc
     8101 gtcaatcgta ggtacacaac tcctggtgta gcaccgaagt cattgatcac gatatctgct
     8161 gaaattggat aacgatcaaa aaatctcgtt aatacattgt tccatacttc gccgaagcca
     8221 tcgcttcctg tacaaatttt tacagtagat tctgtttttt ctgttggaaa catcaagatt
     8281 tccaaatctc ctgaaccgac cactcctaca tggattttct tattgatcgg ctgtttcgtt
     8341 ggatatttat aagctaattg ttccatggat ctctcccctt tttgtaaaac agagcataca
     8401 cgttctgctt attcgtcttt cttcttccat ctgggtaaat ggcgacaaaa ggtttttctc
     8461 gccaatttta tgaccaagtt ctgaatttca ttggtggttc atacaggcca tctgaccatt
     8521 cgatcaaatc atcgatggtt tgtgcagcta ataatgaacg tttcgcttca ctacgtttga
     8581 tgcctagatc ttctggtaat ttcacgatgc cggctgcacg caagctatct agtgattctt
     8641 tcgtgctagt cattccaaga ggtgtaacac cgccaattgc tgcaattgct gcttgtcgtt
     8701 cttcttttgt ttttgctttg tacaaataag caattccttc ttctgtcacg atatgtgtag
     8761 tatcttcgct ataaatcatc actggtgcat tggctaagcc tgcttgtttt tgaacagcta
     8821 gtgcatctaa ttcttcgaca aagactggtt ttttatttga accatatgtt tcaaccattt
     8881 gaacaactaa tttttggcct ttccctaatg gatcatcatt gtcgcgaagt gattgccatg
     8941 ctggtgtaga atgacgacga ccgccaggat tatgtcccat gttaggcgca ccgccaaaac
     9001 cagataaacg tccttttgtt acggtagaag agtttccttc gtaatctaat tgcaaggttg
     9061 aaccgataaa catatcgatg gcatattgtc ctgcagcttg tgataatgtt cggttactgc
     9121 gcatcgtgcc gtctttaccg acaaaaaaga catctgggcg agcagcaata tatttttcca
     9181 taccaacttc gccaccaaaa ctatggatgc tttcaaccca acctgattcg atggctggaa
     9241 tcatcgttgg atgtgggttc aatacccagt tcttcgcgat tttccctttt aaccctagtg
     9301 attcaccata agttggcagt aacaattcga tagctgctgt attgaatcca ataccgtgat
     9361 tcaaagattg tacgccgtgt tctgcgtaga ttcctttgat tgccatcatt gccattagaa
     9421 tttgcaattc tgtgatgttt tgaggatctc gtgtgaataa agcttctaat tgataaggtt
     9481 tatctgcttt cacgacaacg tctacccagt caccagggat atctacacgt ggtaaagaat
     9541 cgacaacttc gttaacttga acgatcacga taccatcttt gaaagctgca gcttcgatga
     9601 ttgttggtgt ttcttccgta ttgaaacctg tgtacaagtt gccgtcttta tctgctttat
     9661 ctgcagctac taaaacaaca tttggaatca aatcgatgaa taagcgtcca taaagttcca
     9721 aataagtatg gatatcacct aatttcattg tgccgtcttc aatcatttgg gcgacacgca
     9781 agctttgtgc accagcataa gagaaatcga tcttgcttgc gattcctttt tcaaaaatat
     9841 tcaaatgttc tggtcgtgag atactagaca tgatcaaatg cagatcattc actttttctg
     9901 catctacttt ttcaagactt tctgaaagaa aacttgcttg tttttgattg tcgccttcta
     9961 atacaacttt gtcgccggga gcaatcagtt tttccattac ttctacgatt ttttcagttt
    10021 ctacatactt gccattgagc aagttgctga ttttttccaa tcgctgcgct tttttttcgc
    10081 gtttagaagc ccagttgcgg tttggttttt ccataattag tcatctccat ttttgatata
    10141 gaaacgtggc gtcatcaata ccatgttggt ctagattgat cgtacggatc cctttatcta
    10201 caccaaattt tgtatgaatc agctttgtca aagtattgcc ggggggaaat tcaatcgtca
    10261 gatccattcc taattctttt gccaagtcca tcatttggtt ccactgcaca gggaaagcta
    10321 ggttttccaa caaatcttta cgaatctctt ctgcgtcctg agtggctctt ccatccgtat
    10381 tttttatata caaacatcta gcatcagata gatggacaga ctgcatatac ggcttgaatt
    10441 tcttgaccgt atcattcatc aagagacagt gagatggcac agggactttc agcagcttcg
    10501 ctgtttgcgc ttggtgccgt ttggcttctg ccaacgtaac ggataaacct tcgatactcc
    10561 cagataacgt atgttgcatc ggacaatttt catttgacag ataaactgga tggtcaggat
    10621 caaatgtttt tgctacaatt tcctcagctt cgcttctcgt cacacctact actacgccca
    10681 ttgcataacc gcttggataa gcttctttca ttaagactgc gcggtgatag actagttccg
    10741 ctgcattctc aaatctcaaa gatcgacaag caacagctgc acaaaaagca ccaatcgaat
    10801 gtcctgctgc aatatcaggc tgaataccgt ttttttccat ttcatctaaa taaaatactg
    10861 cttttgtcag taatgctagc tggataaaaa ccgtatcttg atagttttct tctttatcaa
    10921 ctaaccgata ccccgtcaca ttttctaaac gttccatgta catttcaggg atatccgcta
    10981 acatccctaa cttctgactg ccttgtccag gaaataagaa acctgtactc atgagcctcg
    11041 cctcctcagc acttactttt tccactgcta tcttagtcaa tttctgctat aataaaaaat
    11101 gccaaaaaaa gaaaaaacca taacaaaaag gtatggatgg gatggataat tatgaaattg
    11161 cttcatctgc agtattttaa aaaagtattc gaaacaaaaa atgttaccca agcagccaaa
    11221 gaattattta tttctcaacc tgccttgagc cgtgcgatca aacatttgga aaacgaatta
    11281 ggtgtccctt tgttttacca tagcggcaga aacattgaga cgaccgttta tgcagaagaa
    11341 ttttatccat atgcagtcaa gacattagac acattagaag aaggaatcaa attattgtct
    11401 tcggtcaatg agaagatcgt tacttccgtc atattgtatt tagaggtagc ctctgtttct
    11461 attccgaact tggtccgaat ttttatggaa aagcatcctg atatccaatt aacgatcatg
    11521 caacatcatt taccagatga tattaccgat cctgtactgt acatcacttc ggagatgaag
    11581 cctggcatga ccaatattcc tatcatgaaa gaacctgttt atgtcgccat tcctaagaaa
    11641 catcctcttg ctaaaaaaga acatctgcaa ttagaagata tccaacgcac ccccatcttg
    11701 atgctgtcaa agagcaacgc cttccgtcat acactggatg cagcacttga agcgaaaagt
    11761 atcgaattga cgatcagttc gacgacagat gatcctgcga cattacgttc tattgtcaag
    11821 caaggattag ctgttagctt tttcccaaaa gtttcttggt cttacgataa aaatgatcct
    11881 ttcgtcttgc gggaaatcaa agatttgccc ttgacacgaa caatttattt atcttctagc
    11941 ttcacagaag atcatccttt gatcaaaatg attgcaaaaa cgatgcggga attttattta
    12001 ggaaaataaa aaaaaaagag ctgggacaaa attcttgtcg cagctctttt ttcgaacaaa
    12061 cggtgttcca gaagtaattt cttagaaaat aagccgaaat ttcccaaaaa tttgagaagc
    12121 aattttcgta aattccttct tatttcttga agctaaacat ttctgtcaca acctcttctc
    12181 tattgtccta gacattcatt gcgttgattt aaaacttcca tcaatcaaat ctgctagttc
    12241 atctagcttt tctattttac gtagatcaat agagatccaa tgttctttat tcatgtgata
    12301 ggctggaaaa tacccctctt ttcctcgcaa aattccgcca agttcaggat cgaccttcaa
    12361 atcaagaata ttgatgatcc tatggtcact tgcatcatca tagatttttt cttctggaac
    12421 agacatcagg accccaaacc attttttgct tttttgatgt ctgaatacgc aataatcagg
    12481 aaatttttta aaaatatatt ctggagacaa attgtatgtt tcattgatgt gatcgattag
    12541 ttcttttttc gttagcatct gcttccatct ctttcttatt gattcgcttt attcaaagat
    12601 accacgtaag aatagaaaaa tgaatacaag tagttgctaa tcaaatactt cgatattttt
    12661 cttttttatc aactcttaaa tgttcaatcc gcctgtgttg gtcttgctac catctactat
    12721 taaaatgttt attattgttt gcccacctat tttttgattt tagtggtatc atttttcaag
    12781 ttcatgatga aattcttatc atcagtaaat gctccagaaa aataaacaat gattgaaaaa
    12841 tcagttgaaa cttgaccacc gtttattttc tccgtattct tagaatagaa gggagtctga
    12901 ttgatgtatt cactaatgaa aaaaatcatt accgaaaaag atattcgacg tcatgtttct
    12961 ttagtagaac aattgctgaa tcatacaaag ataaccgtaa atgaattagc tgaaatcata
    13021 gggacaaccg aacgaacgat tttttctgac cttcaatcta ttcgctctca gctgcccgaa
    13081 ggctgggaca tcatcagcga tcaagcagga atcagtttgc aaaaccagca aaatttatta
    13141 acaaatgacc tttgggagat ttttttcaaa caatcagtca gtgtcgaact gctgaaaaat
    13201 ctgcttttta caaaaaaagt tgctgtccct gattttctag ccgaccatgg gctttcttat
    13261 gggacattga aaagacatgt gactaaaatc aatcagcgtt tagcaagtta cgacttgcag
    13321 atcgatttga cgaaatatac agcttgtatt cttggaaaag agcgtgcgat tcgtactttt
    13381 tatcatcgat tgctgattcc tttcactcat aacaactatt ttttcgagga ttattcgatc
    13441 catgaatccc attattttca atttttaaga aacctcagtc aaactgaact agcggttgaa
    13501 acagaagaga ttttcggaac atgttggttc tttatcaata cgatccgtat aaaagcgaat
    13561 tgccgattag attcttctat ccataccaac agcacactct cctccctgta tgactctgca
    13621 ttgaaaaaat tgtatttaac ggaaggtatt tatcttaaag acactgagct ttcttttgct
    13681 tctttctgct ttctggaaag ttggaattac aacaataact atggacaaga aaccgctcgc
    13741 tgcctgcatc attctccatt tttagaggtc ctcgaaacgt ttgtagagga actagcttca
    13801 gagctgtctc ttgatcaact aaaaaaaaca tctctgactg ataacttggc tctcctcata
    13861 ttaaaatatc atgaatcgcc gatattgatt gaagcattgg accgtcaata ccatcgtttt
    13921 ttagaaacat atgatcagca atatgcacac ttgtatcaac agaaagagaa tttactgaac
    13981 gatctaaaaa aagaaataga aatcaatgaa ccaaattact ttctccaact cttgtctcta
    14041 ctgatccaac aaacgatttt ttctatcaaa ccgaatttgt tcaatgtcta ttttattttc
    14101 caaggagaac cttcatggaa ggcgttcttg cagcaggaat taaaagatta cttaggaaaa
    14161 cgtgtctgct tcttacctat cgaactaacc gatctttcca ctatttcttt tgaaaaaaca
    14221 gatatcttga tttccaattt ccctctagat tctctagata tccctattgt atacatttct
    14281 tctattccaa cgaaaaatga gctcaatcgt ttgactgaac tcactttcaa aactttttta
    14341 tagtaacggc tagcgactgt attctctagc tgttttttct tttttgcgaa acagtttgtt
    14401 catgatccct ttttctattt ctacaactaa gaatagaaga atcacatttg ctccaatcaa
    14461 taactgttgt gtcatcgtta aacttactgt accgatcagc tgctggccga ctggtagaaa
    14521 aataaccatc ccttgcaata aagcaaggat acctaatgaa agaaaaagcg ctttgttttg
    14581 caaaagacct tggttcaatg acggcttgga caactctctg caattgatca tgtaaacagc
    14641 ttgagctaag acgatatttt gcagaagaat ggtttgctgt aagctttgcc cttcaaactg
    14701 catagccaat aaataggcag gaaccatgat cagtagggag acatagatga tccgaaacaa
    14761 atgataacca gataggattc cttgctttgg atctcttggt gggcgcttca tgatttctgg
    14821 atctggcgac tcgaacccta gtgcataaga taaagtgatt gtcgttacca tattgaccca
    14881 taaaatctgt actggggaca aaggtagcgg gcgattcagc ataagtgccc aaatcaagat
    14941 caatccttga gctaatgcag ttggcaagaa gaaattgatg gttttttgca aattatccaa
    15001 aatccgccgg ccctctttta cggcttttgc aatcgtatgg aaattatcat ctcctaaaac
    15061 catatcagct gcctgtttgc ttacttcact acctttgatt cccatagcaa ttcctacatc
    15121 ggatcttttc aaagcaggtg catcatttac gccgtctccg gtcattccga cgatttctcc
    15181 atttttctgc aaagctttaa caattcgcag tttatgttct ggtgtcgttc gagcaaagac
    15241 atcgactatt ggcacttgct gaatcaactc ttcatcagac ataagatcca aatcgatccc
    15301 ttctaaaact ttggcagtat gttttaagcc aatttgttcc gcaatcgctt ttgctgtgtc
    15361 tttgtgatcc cctgtaatca ttttcactga gatccctgct tgttggcatt cctttacagc
    15421 tttgactgca ctttcttttg gcggatcgat caatccggcg atccctgcaa aagttaattg
    15481 ggttaaagtc tcatgcgtga gttcctgttt cgaatctact tttttataag caaatcctaa
    15541 aacacgctga ccttttctcg caaattctgc agctgtctga ctccatgctt cttgttctgc
    15601 agaagaaaga gttgatttag caaataatac ttctggtgca cctttgacgt aaatcaccgc
    15661 tcctttttcc tccgcatgca tcgttgccat atatttgtat tcagaactga acggaatctt
    15721 tgcttctatt tttttcaaag gtagattggc tgtttctgtg tgtcgaatca gcgcaacttc
    15781 tgtcggattg ccacttaaat tttctgtttt ctgttcttct tttgttttta attcctgaca
    15841 gtttttcata atggacaaca cgcaggcttc ttcttttgca gctacatcca caactgtcat
    15901 ttcatttttt gtcaatgttc ctgtcttatc tgaacagatc actgtcatag aacctaatgt
    15961 ttctaccgaa ggaagtcctt tgatgatcgc cttttctttt gccatttcat gtactccaac
    16021 ggatagaatc atggtcaaaa cagctggcaa tccttcgggt accattgaca ccaccaaagc
    16081 aatcgtagcc gaaagaagga tatgccaatc cataccatat cgaaatgtcg taaaaataac
    16141 taagaatagg ctcaaacaaa gaatgccttg aaaaatttgt ttgtttaatc ggttgatttt
    16201 tttaatcaga ggcgtcgttt gttgttcgac cgattgcaat gcttgattga ttttaccgat
    16261 ttctgtcgtg tttcctgttt ctacaacgac accgactgca gaaccagatt gtacaagcgt
    16321 tcctgaaaaa cccatatttt tctggtcgcc gatcgattct tcttctgtca aaacggcatg
    16381 acttttttca gatgcttctg attctcccgt caaaatcgat tcttccacta ataaatcatg
    16441 tgcttcgatt aatcggatat ctgctggaac gacatctccg gcacgtaaga caatgacatc
    16501 tccttttacc agttctttcg cagggatttt ttcttgcatc ccgtttgata gtactatcgc
    16561 ttcttgtccc atcagctgct tcaaaccatt caatgattct tccgcttttc tttcttgcca
    16621 gtaactaacg agtccattga cgatcactac gaataaaata atacttcctt caatatattc
    16681 actagaagca aatttcaaaa aagaggcaat gatcaacacg atcatcagca gatcagtaaa
    16741 atgctttgct atttttttcc atcgttttgt ctgctgcctt gcttttattt cattatatcc
    16801 ttgatcagtc aggcgtttct gtcgttcttc ttgcgacaag ccttctgttg atgtttgata
    16861 gtttttcaat acatcttcta ccgtcaatct gtaccagttc atcctatccc ctcctgttta
    16921 attgatactg ttattatacg cagggaagtt ttttcaggcg tatcgattat ttcagggggg
    16981 tctgaaataa ttcgttttgc aaaatgtatc tgggcctaac tgatatttct attgcttatt
    17041 cttttatata caaaatttaa gaatacaaac cagataacca ataaaaaagg aataaagcgt
    17101 gtcgtgcttt cgaataacaa gctgaaagca attgaagtaa aaaacaccaa caaagcaaga
    17161 tctttatatc tttgtgtagt gttccgtttc ttttctttaa taaattgaac atgtgcacac
    17221 aagatcgaag accacacaaa tagaaaacaa ctcgttgtca tgcctgccaa aagctgaaaa
    17281 aacaatcgac tatcagctac aaaaaaatgt agacaaagac cggcaaaaaa taacattcct
    17341 gtaattaaaa taccgtgtaa aggtacgcct ttcttagaaa gcgcacaaaa ccaatggggc
    17401 aaactttttt cttttgctgt ttgatataaa atcctgctag tactgtagac tgcactattt
    17461 gctgaggaca aagaagcaac caaaatcatt agattgacaa ttgctgctcc gccttggcaa
    17521 tggatcactt gcagcatagt cactaaagga ctttctttta aatctaaaca atgccatgga
    17581 acaacaaata aaatcgacaa aatcgttcct atatagaaaa agagaatacg ccaaggcaga
    17641 cagttgattg cttttttcaa attcttttgc ggatcacttg tctcgccaac ggttaagccg
    17701 atcatttcaa ttccagcaaa actgaataaa accataggaa atgagactac cattccagca
    17761 aagccaaacg gtagtaattc atcgatctgt ccaaaatttt ctttgattcc gatagactgg
    17821 gtatgaaaat ctgagacact caatcctata cccaataaga taaaaaggaa aatccccaag
    17881 atcttgatga ttgagaaaaa agattccaat tcaccaaacc acttgactgc aacaaggttg
    17941 actgccatta agatcattag tgcgctacct ccgcttatca aagctggtac atgtacaaac
    18001 cagaaatgga tatacaatcc tattgctgtt aattccgcaa gtccagctcc aagccagcaa
    18061 agccaataag tccaattgac aaaaaatgcc cactttttgc ctaaataata gtcaacgaaa
    18121 tcactgatag agttaaatgt actatttgct aaaaataaat cacccatggc ccgcatcaaa
    18181 ccataaatag ccaaaccgca tatcaaataa acgagaataa cagctggccc tgctgatgca
    18241 attgcctttg ctgaacctaa aaataatcct gttccaattg cgccacctaa tgcgattaac
    18301 tggatatttc gattagttag agtccgtttc attgccggtt tttccatcaa gttcaccttt
    18361 ttctttttca agcagcatgg cgacttcctg ttcccaagaa ggtttcttta ttttttcaat
    18421 acagagtggt actacaaaca aaatgacact gatggctgtg actgcaatta gattgatcca
    18481 tgttccagct ttcatcgtca gtccgacaga aaatccgatt gccgcaaata aaacaataac
    18541 gacagtccaa actccaagat tgctcttttt ctcttttttt ccaattctaa atggtcgatt
    18601 caactttgca tgtgtataac gcaatctcaa aactgcgatc cccatgatga catagacgat
    18661 acagtagata acagtagttg aatttacgag catcaaaaat gcttgattga cgccaggaat
    18721 cagcaaataa aaaagtgcaa agattgagat aatgacagac tgagtcaaaa taacggattt
    18781 ggatacgtca aattgattcg tcttccaaaa attgaatttt ggtggatacg caccttttct
    18841 agcactttct gtcactgtct ttgctggtcc tgaggcccaa gcagataatt ggactgctac
    18901 accaatgaag acaagtaaac taaacagatt cacaataaga tgtggcaaac caagaatttg
    18961 gcaataaatc gaaatagatt gcgcgatatt attcaattcc atttgtcctt ttggtacgac
    19021 atttgctaca actaaagcat tcaaaatatt gacgaaaaat ataaaaatta acgcggcaaa
    19081 tactccgcga atatacgttt taactggatt ttccaatcga gtgatataca cagaggacat
    19141 ttcgatacca gtaaaaataa acatgatcgg cgcaaaataa acaaatgagg atgcagtcgt
    19201 agtatctggt attaatttgt cccatgaaaa actgcctaat gttccggttg gtacgatccc
    19261 aactttgatc cacgcagcaa accctaataa gagcatcatc gtcaaaggca cgtacaatcc
    19321 taaccaaaca ccgacttttc cgccgatttt tgccatatca aatttcaaat tcaaaaaagt
    19381 gatgatccaa taaacgacta aaatgacaac taatgtaaat ttgctgttca aaccaagcgg
    19441 cacattatcg ataatattac caaacaacgg agcgagtgct gaagcgacca tcaccatccc
    19501 aggaaacatt tggacccata acagccatga gacaacgaac ccccattttt ttcctagagc
    19561 attcgatacc caaagttctg gaccaccttt ttggggaaac attccagcaa attcaccaga
    19621 gatcaatgag attggaaaag cgtacaataa tactgcaacc agcatgtaaa aaatcatcgt
    19681 ccagctcgtt gctgctacat ctggaatatt tcgtacactt gctacaaggg cgcaggtcat
    19741 tccgataaag gtcatggtcg tgatttgctt tgatgaacca gtatctttca tctttattcc
    19801 ttctttcaat agcgacatat gtttttgttt tcttttattt ttgttcaaga acagaaatga
    19861 caaggtttca tcgattcagt acaaaaccca gtatcaccga aacatccatt gaacgatctt
    19921 agtgaactgg gtttgataca tgaaacgaac gatccaactt atagaacgtt ttataaaaaa
    19981 gaatatcttg gcgaggaata tatgaaaaac agggggaaat acaattgttt tgatgaagca
    20041 aataattgtc tgttctccat atgtctcttg ttattctttc acacaaaaat cagttctatt
    20101 ggatgatttc gtttagtttc ttttccattg ctttggcgat tttttttgta taaaaattga
    20161 aaattcgatc atcattcaaa taaggagtca tgatagccgc acgtagcaga gtgattttct
    20221 gttctttttt ccattcttct ttcgttagac ccattctttc aacaaaacga atcggagaat
    20281 caccgtaatc ttcttgtgta aatgtagtat gagaagtaat aaaccgttca ccataaacat
    20341 cgccatccat ataagaagac cgatcgaaca ttttttcatt taattcattg atagcgttca
    20401 aatctgtaca gccttgttct ttgaataccc aattgaccat attaaaatca ggatgattca
    20461 atggatagac ttcgattgtt tttcctttaa cagtaaaagt cagatgatcg agaaattctc
    20521 tgaatctttg agctgcctcg attgaagccc caatcaattg accatatccg gtcacattca
    20581 atggcaatac acgatgtgct gtccagactg cggcggcggt tgctccagct tttgatcctt
    20641 ccaaaatata agctcctaac atatctggag cctttactga tttttcaaac acatatggcg
    20701 cgaaataaga aatgatattt ctcatatttt tatgtttgat tacgattcct ccagcagcat
    20761 aagggacata gcccatctta tgaggatcta tcgtgacaga atccgcttct gaaatagctc
    20821 tgaacccttc atatacctct ttgtcaatcg ttacgcagtg gtggaaaaca tgatgttctt
    20881 caaagaattc agctaaggaa gcatacggca caaattctcc tgcttcgttt agaaacagtg
    20941 aacgcgcata gccgccatat gcggcatcaa catgtaaata gaaatagatc ccttcatctt
    21001 ttaacctttc tcttaattgg acgattttat ctacactgtc tacttgacct tcttctgtcg
    21061 ttcctacgac agcaaccaca cctagaattg ggattttttg tccagctaat tcacgaattg
    21121 ttttttccaa agcattaata tccattcgat aatcctcttg aactgggata gcaaccatct
    21181 gatctaagcc gactccgcaa atatctaacg ccttcatcca agaatagtgt tttgtttgag
    21241 ggaccagcca tttccctaat ctttggatat tttttccgct acgggaagaa gctgctttga
    21301 cctcatccat ttcttcgtcc gtaaagcttt cggtcatttc caaaatttct tcaacagata
    21361 aattcaaaag ttcccactca gacatttttt tcactttttc aggcagcact tccttgacag
    21421 ctaaaggaat cgatttgatg caacgggcgt accataatcc ttcaagatta gcaatagaac
    21481 catctgctgc aatgtgtccc catccgtctg tcatattgaa taatgaagca aaatcctgtc
    21541 ctacttctgc ttccatttgc gaagttgcca tagaagattc caatgcaaca ttatttgggt
    21601 tccacagcat cgcatagttg taagctaata aagcaggcat caatgtttca gcattcattt
    21661 gtccccagta acggcctgct gaatgccatg ggatcgatcc tgcgcgtaat cgctgactga
    21721 cttcatccaa gacctctatc atatggtcgc gggtggctag ataatctgga gtgagcttat
    21781 ctccttcgct aattgcaggc atatctgaag gaatataatt ttctctccat ccaagatgtt
    21841 catctaccat cttgttcaac aacattttat aaactggtcc attttcagct ttgtcgccta
    21901 taaagacggc cttgatatcc atatctttca tttttcttcc ctccatattt tgttggtcaa
    21961 tgtcttgcag atacatgtgt ttatctgtaa cacctcttca ctatagcgcc aaaaatgaga
    22021 attttttttt ataacatcaa atccacgtag atttttacaa acaaaaataa ataaagtcgg
    22081 atttttctga taattcaacg caaaaaagcg aacaattttt caataaatca gtcttctaaa
    22141 atagataacc taaaaaaagc gaacaaacaa a
//
LOCUS       DAZKKW010000044        20988 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.044, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000044 DAZKKW010000000
VERSION     DAZKKW010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20988
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.044"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            190..990
                     /locus_tag="ACG8PO_001600"
     CDS             190..990
                     /locus_tag="ACG8PO_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0206912.1"
                     /translation="MVEISKTALDSLAKLFSSRFDGAEFVEYLSQNESGKTLIETLKK
                     DYLNENDFPTWTNIEDATWDVVCTIYSPWVTIADFVLDKIEGDTYPDAKSLATSDFKT
                     FYGYLMGLIGNGIDLKVTPKQTPEEIEKEKGEVQMAYADDREKVKLLEGKLSEEELGK
                     MVQEINEDSNYFPELKVYQNDEDFFNEMFSNSPWEAVSKTNLGEWNFQDDLVAFSNDG
                     YLRSYSESEYRAELKTYETDILMSYLNLLESGQGELRDKVEELLKEEL"
     gene            1096..1884
                     /locus_tag="ACG8PO_001601"
     CDS             1096..1884
                     /locus_tag="ACG8PO_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0206913.1"
                     /translation="MSATTDMALEAVIKDNTGNVENVEDFSDIAINLSAWISAVGEEN
                     IEFEIKNMDDLKAKFPFIASKLSQFAEEKSWNELVSFSEKIYDITQEDECVSWEMNNQ
                     YKKIDAFVKSRIEGTAFEFEGIDTADNWLNKESFIKSIADDLEKQWSKEDYAEYLKTE
                     RDTEVKEKLTEVCLDEHIDPNIVKQEDYEVVIDKVPTDFEEVVKYIVEEALSQYSSDE
                     EVDEETVRTRVWQLVFDDKDYKYLSDQNSVNWSVRLLIDQNDIE"
     gene            2116..2985
                     /locus_tag="ACG8PO_001602"
     CDS             2116..2985
                     /locus_tag="ACG8PO_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HFQ0206914.1"
                     /translation="MKKKEFSRSLLGILVLVAVGLSGKGYFQNNSTQEHDFQIKSSVQ
                     VGNKADSESKEFLGIRYDPNKYPNNIVNINDGKYTLTKEDHEALSEKGSQTFWVNYAD
                     LDQLGRAGDVEALITYDAVNSHSSKNVERPSFKSSVHIAGEYKDGVFDQERDTWVGKD
                     SNNEITQLEGYKGYLYNKSHSLAWSLGGDMEVHNLTLGTRAQNVGKNDQNGGMAYSET
                     IVRDAIYKNKNEKVLYKVDPIYKGNELVPRGSHVEAFSVNDNGKTVNLNVYVFNNQKG
                     ININYQNGTWVRD"
     gene            3005..3352
                     /locus_tag="ACG8PO_001603"
     CDS             3005..3352
                     /locus_tag="ACG8PO_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0206915.1"
                     /translation="METISINGLDKSVANRIKKGNGYYLLKKGKYSVARELETMSIVY
                     VAKNHFDVEQAIFDPDSEIGKCVELSEKTVSTKGAMIIEREMLDEIRVIIPKDTQMCE
                     DSISDDEPKSERK"
     gene            3365..4627
                     /locus_tag="ACG8PO_001604"
     CDS             3365..4627
                     /locus_tag="ACG8PO_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0206916.1"
                     /translation="MSEKSIELKEYKRSDGEKYYPVASFSKSVYLVGRALVVGAIGIY
                     ILHWVTKVVQLLVDTLVSILVSLNDVNGTDDLGLKEMFKELLNAFFPNYLSYSYNVQS
                     FFAKLHLVDISILVLEAGTVFFVGYLVTLMFRHHYGEQRPFFDDMESRRLKREALEAM
                     DVKYDPNKIIGDETNNKKRKRNKTQLESDVLSTLKNIKISVHTRRDLSVNEDIREYIV
                     KIPQPKGKKVRETVLRQIQDLGSILTRATSGRAKFGEKEDEVSGDYFIFRASVNIERE
                     RKERVSTKNKETERATSSIAVSNSQFSFPINLFADNSKNIQNQTEKARKYAETQKQSL
                     ELYLSSADVPASLTDMHVGNTSIEYLFTLSRTRKIQLSSMKEEIETYLHISGVIVRIN
                     AGRIQITLPLGENDRIPIDVPSMINSVF"
     gene            4707..6083
                     /locus_tag="ACG8PO_001605"
     CDS             4707..6083
                     /locus_tag="ACG8PO_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFQ0206917.1"
                     /translation="MAMKLLEKVKKRAEKAESILLDKEPNIKLNGKFSVNMLNTKAKD
                     PTDAIFGIKPDGTIAHFCISEMPHMLVAGTTGSGKSVLLNEILVTAMCHSTPDELKIG
                     IVDPKRVEFGRYKKLPFMLADPITDMDEAYDFFEYLVILMHERYKLMEKAGLQNITLY
                     NEYAEKNGLDRFPYVILLVDEYSQLVGTHKEVEGLIVQLGQMARAAGIHVILATQSPR
                     STVVTGIIKANFPSRVCLMVASDLESRIVLDEGGGESLSPKGDMIIKLVNGSMVRAQG
                     AYISNKEIETIFNHLRNTMPEPEYVDYRAIVAESRGEGGEDEDFGGIGKAPVRKSAKR
                     NLNTPLANSSSKEPSIEKKSTFALKLEKNKEEKEKQGAKTLEKLQAEKLEKVLKALDK
                     ADSEPQTSNNTNNEQPISSKTKPKTTDPKVSVNVTITTDKSEKKQVNGTTSPIMQKML
                     KAKKQVHK"
     gene            6080..6709
                     /locus_tag="ACG8PO_001606"
     CDS             6080..6709
                     /locus_tag="ACG8PO_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0206918.1"
                     /translation="MIIVSSFLRWLTPYKYTDEVVVKRTKKRDKKAVLTPKQMLDHGI
                     LGVKVLEVNKPFESNSAREKLLGAEADVAYFYHDKSKHQIAIKFNPKSAWRYQARDDL
                     KRRKLRPMLYPEGKEAFDRTHIVPIGFHGTESDNRLLVGWNSNQNRVQINKFEKKVRK
                     FNDRKTILWFADICKQPDGTAKWEAVIFDENGKKVMSETWHDKAKFVWQ"
     gene            6728..7207
                     /locus_tag="ACG8PO_001607"
     CDS             6728..7207
                     /locus_tag="ACG8PO_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0206919.1"
                     /translation="MLELFEANRTGFNQKLEVGETIEYKDKNYIIIGIYNTRINRIGD
                     PRITSDLVCQSVNYSPDLTSRYKKYVLLEYRHKITDEIGVNNTRNIFKIGTVIPYSNN
                     EEKIFYQIYGIEKFEYEHVDLLVTYQMRLIEPWSQAEIDKAVKLNRLSKFNVLGNAF"
     gene            7766..8365
                     /locus_tag="ACG8PO_001608"
     CDS             7766..8365
                     /locus_tag="ACG8PO_001608"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HFQ0206920.1"
                     /translation="MREKNKNSSRYLVATVVFVCVLIFLFDFLQIGKSIIGNTLSAKE
                     MVALGGLYEGHLFPNIFISIVSHINLYHLISNLLFLVVLEEALNDQYEWLTLLIVLII
                     SGFLGSLVPFLLHSNIVSMGLSGGIFGWIALFAIAYIRFDSKKHLLLSVFVLLIIGIL
                     LVSTFESGETNVLSHVIGFVCGMILGFILKNVSLIKQKN"
     gene            8381..8857
                     /locus_tag="ACG8PO_001609"
     CDS             8381..8857
                     /locus_tag="ACG8PO_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0206921.1"
                     /translation="MPKKKEKKIPVFLSVSEMLSLQILLRKKIETIDEEVRNKRAMYE
                     QREALRERIHYFDDIHTKIYEARTNHEDESKLGLAQQRIFEWLNTNTEQEPLRAISNL
                     FEFGTKHWFNAVEKDADLQEAFDTLSEDSKSMRDLLITYLNEHEFEQTKVKKKNKK"
     gene            8874..9776
                     /locus_tag="ACG8PO_001610"
     CDS             8874..9776
                     /locus_tag="ACG8PO_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0206922.1"
                     /translation="MLFTLLGYLCLVLAFFGAIAIFVGQFQANNPIMRNIGIGIVIIS
                     LIVFGATKIFGSNDQQFGISGVDNSYAPDSLGRVVIDGTFENVTSYKNVSLTVKEDDK
                     KASDDVLATIKPSQSQFEVFYTVKENNPVRKLTLVFNVDGEEYYKDVKINKASTKKIV
                     ESAKKEVATEESTTESSSTVEVELPDKIDFDPEKVKSFESVSGSMADRFNTEVFKAIK
                     TVKLDTEKQRINVTLFNAVESMDQDKKVTVARELGKRVIGLYRGTAKPDSDGSDLPVH
                     VVYEDGKVFGDSTANEALTIKLAK"
     gene            9849..10043
                     /locus_tag="ACG8PO_001611"
     CDS             9849..10043
                     /locus_tag="ACG8PO_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0206923.1"
                     /translation="MVVNKEKVRKYVTLKIASVEYLEKRAKEEEKTRGKKVTVSELLE
                     ELIESDQTIRRAFTNRSVKS"
     gene            10148..10576
                     /locus_tag="ACG8PO_001612"
     CDS             10148..10576
                     /locus_tag="ACG8PO_001612"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0206924.1"
                     /translation="MIIQKFDWKMKLGISLVILGFIIFLGIKAQKNQSTFETMSSQII
                     EECSKIYYCENKTEESNGFYYIDQPHDVVIKMESHEVNEWIQRSTNVSKKLERYSQTF
                     LSKVIFSNCKNEETSQRVASSLENLKFSVSKKEKFVDLRK"
     gene            10811..11398
                     /locus_tag="ACG8PO_001613"
     CDS             10811..11398
                     /locus_tag="ACG8PO_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0206925.1"
                     /translation="MENNNDIRYFSIGERVTTVSELSKDYRDKEGTIISLKKNRRGVW
                     QYVVDLGDGVTVECKDSQLIRNDGSHSIEKAILVFPKVSSKIMNDWLLGKFGEYNKKE
                     IAEVIGKSYPTVLGYLKKITNKEAKVEDVLTVRELQSLIEYRNKQITNPEYLKKEDGL
                     LKQLEQVNKIRKMQKVAPLSLDNFKKIYLKEIEKD"
     gene            11398..12297
                     /locus_tag="ACG8PO_001614"
     CDS             11398..12297
                     /locus_tag="ACG8PO_001614"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0000400 - four-way junction DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFQ0206926.1"
                     /translation="MWSVTLPFQPVSAPRPSARRNVADATNDVAEKRISTYYDKKYLD
                     YLKKIKSFIEDQGLLDDEFYSIVNDPMGAIMEVDFYYQIPKSYKKVYNIMKTTAPDLD
                     NLVKSAMDGIFNISAIRDSHITGLIAFKYNVANEGKTVVRIKRYSEFEWDTSEGLNGD
                     IWEATFDFKPVATPRPKSKFRGNGIITYYPKEYNDYLENIKNTLKEKDLVNDQLKKVM
                     NAPMGVIAQIDYFYQVRKDKKKLDSLMRTSQPDIDNLVKGTLDGIFNKEIGIKDSRVV
                     GLIAFKYNTFEKSKTNVRLQEMG"
     gene            12297..13211
                     /locus_tag="ACG8PO_001615"
     CDS             12297..13211
                     /locus_tag="ACG8PO_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0206927.1"
                     /translation="MNNNEKILHVLDSFETIQEELKKYRDVLEQRYDFVNQQKSNHMD
                     FILNMNDLKKKLVERKEQEKLIKAYFELGEKEVKKAMELNEDRRVLDQLLEQLLVMFQ
                     KGRIDEDLIEEGLRKYPANSGIGIVLKAIDEEEIEDFIPAEDFESAMEYIKYYSQGIT
                     AFREFDPEDVIHDLNNLKEWCEGYEVDDSGLDYLISIMEIEEEMPDKPDPTDILELIH
                     DARNPIAYISRGYTVLEYYKPYISAMNHLRRVLREKREYRSVLNASNRLEKAVSELDA
                     YYREHYLQAGGMPRNTKANISRYIKEAE"
     gene            13263..13478
                     /locus_tag="ACG8PO_001616"
     CDS             13263..13478
                     /locus_tag="ACG8PO_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0206928.1"
                     /translation="MKRDVFELYVLERLEDGMMAQEIIDEIDDMISRFGQLELNDGNK
                     NSVVLQQFWLNNFSYKDFIKEKANRLY"
     gene            13669..13923
                     /locus_tag="ACG8PO_001617"
     CDS             13669..13923
                     /locus_tag="ACG8PO_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0206929.1"
                     /translation="MKKEELIDMFQIVERANNMGIMFFDRISLKMDLSVAHQEFNLRL
                     KALLISDDVNFAHDVVGIQNHIDRENKRMGDGFLPRYSSL"
     gene            14102..15151
                     /locus_tag="ACG8PO_001618"
     CDS             14102..15151
                     /locus_tag="ACG8PO_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HFQ0206930.1"
                     /translation="MKKFLVGILFTMGLVLLSPSVSAESSISMYRMYNPNSGEHLYTR
                     SVGERDNLKRYGWHYEGVAWQAPTSGSPVYRLYNRYNGEHFYTVDAGERDKISHTGWT
                     YEGVAFYSYNGANRVPVTRLYNKYVNWHHYTLDQNEKNVISKQGWKIEGTGWYAMPNS
                     APNPTPAPAPSNEKLLNVPVIWQGNTMLCEGASLLEGLHYKGVATNQNLTSFVNYMPR
                     ANDNNPYHGYSGEWRYNVSGTYQGIMPDPVVKWAKNVGGNAVNITGSGTNGIKNEIRN
                     GNPVVTWITYNYASPQFKQMPWGRAVWNGHVVLVDGFKNGAYHVVDPVFGAKWINAST
                     YERAFNVTGMAVAVR"
     gene            15326..15556
                     /locus_tag="ACG8PO_001619"
     CDS             15326..15556
                     /locus_tag="ACG8PO_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0206931.1"
                     /translation="MNQRRRKRLEEISERLSELKDELEVIQEEEEEYKENIPENLQGS
                     ERYEKAEESVDQLEEAVDGLSDILELIEDIAS"
     gene            15592..15816
                     /locus_tag="ACG8PO_001620"
     CDS             15592..15816
                     /locus_tag="ACG8PO_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0206932.1"
                     /translation="MIDISLIEKLLFSNKYTAKELATRWQISESAIKAYRQNKGSVGW
                     RDWKGISIGKAEDIMKREGFQADNDSKKVE"
     gene            16033..16908
                     /locus_tag="ACG8PO_001621"
     CDS             16033..16908
                     /locus_tag="ACG8PO_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0206933.1"
                     /translation="MTTNKRWSELVQNTVKKVMPDGYHFNYIQCSQTTESIYFSILNE
                     NRLYYFRVSSHHRKKREDNVTTFLSGKYNGFKELSEAIGEYLKSNRFVRISDKHILIL
                     LLYLNNSFKLFWRDDTFYYIENGMDIYSGINGILKHYIKALLRYGVLYQEDLSNQIKI
                     SNSGKSLLAKYKKLLSPIYKGIDMEAVNSETLFNLVVDEKFANEFFKSKKIERLNNLF
                     PENAIKAYVDNNKSRLYNWFIPKRLRGKIKIGDQVKVINLNHTKIVTVTELYIAEKEE
                     ITKMKPVIKILDNKE"
     gene            16918..17712
                     /locus_tag="ACG8PO_001622"
     CDS             16918..17712
                     /locus_tag="ACG8PO_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0206934.1"
                     /translation="MVAKKNTLKEASEKYQDMKRRGLIPKDKKGIWREFVKEVSSDRS
                     GGDFVGRYTAKGYSRRLRITVEYGTNESATELKQRYLDTRREASKKYDDFKSRGLIPK
                     KKKGIWREFFKEEDSNRNKGDFIGRYTAKGYTKNLGIKVEYGTGESSWELRNRYLNIL
                     LEKYKAELLELFDVNDFIEMAEKYGHEEGFMINDIMVIDNFTDLLALAKKARDLRETQ
                     MYLFRNKNGRNRRTESSEIEDYYDAFTHDLGAFVDEISGVMNKYRV"
     gene            18011..18859
                     /locus_tag="ACG8PO_001623"
     CDS             18011..18859
                     /locus_tag="ACG8PO_001623"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0206935.1"
                     /translation="MIEYDFNEAYSRIMRNYPLPSNTYLKAKYNTDQAILRAINRPKV
                     RPYENLKTYMLFLVNFEGTAKEGTYANPKSQSMFADYGENPKTANEPIWLSEIELTLI
                     LDYYDIKEMEVLDSHTFLTKKGLLKEYFERIEPLKTDPDTLKLYKNLRTKVFGAIGQK
                     SLREGETNTFDKYSMYNRAYSSMVAGVFRDRMVRYEQKYVHSAYGLLKIRTDGLYFRS
                     EVPEFEELYKLGVVKKKVHTISKEEVEKALEFKNKGFENKGSFGNSKDEVKGVVIDDD
                     FEFDFD"
     gene            18892..19212
                     /locus_tag="ACG8PO_001624"
     CDS             18892..19212
                     /locus_tag="ACG8PO_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0206936.1"
                     /translation="MKKKIVEGNVGVIILKDSTDYAKSMALKDEKVARMDKDLVEFLM
                     DDTERLKGDQFLSLMKDKGLNTYGLFDYKKLAIEWVKEGEIFFIEEWEGTENLVLHQY
                     LLKA"
     gene            19467..20360
                     /locus_tag="ACG8PO_001625"
     CDS             19467..20360
                     /locus_tag="ACG8PO_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0206937.1"
                     /translation="MRTHYIYLEDKIIDEIPAKSREQARKLFIEKYKEINGDSEVPKV
                     VVTVRKRQTKKRSTFHVSPDIYNLIERHRSEDNISISQYIATVIDELISGKYDYAFDF
                     EDEKDYKSVAVFQSDFEKLKNYAGRRNVNFARLLEEFYSEELGVGNEYKRETYAKLSH
                     KLSQALKEVTEVKAELDAKLEEVKKYQTERKNNMKNQVEFDEQKEFEKARKRYNKIMN
                     ELSYEHLTVHEELSEGTEKYTIADMVNEVETLLASFFENGHVNDEWRKEEPKRVNAYI
                     ARLKRFIDKYKENSDKFSKDK"
     gene            20380..>20988
                     /locus_tag="ACG8PO_001626"
     CDS             20380..>20988
                     /locus_tag="ACG8PO_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0206938.1"
                     /translation="MPEIEGIFSPKIGKKQLDFSSFPSVNRDYNLGDEVQILNIDFKE
                     LDFAVAVSNKPKALERVKKIYQENDQNIKNMIKKYRRDNSKVEDVLFNGTAKHDLNVY
                     MALGVALYCNSINQTDILVNIVRNSYQKILKISSETNPYYAMKYLGDNLLNDKKLMHN
                     TEELLSNLAAINFLFKTGSEDYFIDNVLKGAYGTAFYPLYNMK"
BASE COUNT         8054 a         2738 c         4309 g         5887 t
ORIGIN      
        1 aattttttta tcagaatatt tatcagaata ttattagaaa agataaactc aaaagcttaa
       61 tttaataggg ttattggttg tatttagaaa taatgtatgg aaaagctata aagcaataga
      121 taagatgtag aaaatcttat ttgtgaatga aaaacaagtc atcgtaaaat attaatagga
      181 ggtgtagtaa tggtggaaat atcaaaaaca gctttagact cattagctaa actatttagt
      241 agcaggtttg atggtgcaga atttgttgaa tatctaagcc aaaacgaaag tggaaaaact
      301 ctgatcgaaa cactgaaaaa agactatttg aatgaaaatg actttccgac atggacgaat
      361 attgaagatg ctacatggga tgtagtgtgt acgatatatt ctccttgggt aacgattgct
      421 gattttgttt tagataaaat tgaaggggat acataccctg atgccaaatc tcttgctaca
      481 agtgacttta aaacgttcta tggttatcta atgggtctaa taggcaatgg aatagatttg
      541 aaagttacgc caaaacaaac acctgaagaa attgaaaaag aaaaaggaga ggttcaaatg
      601 gcttatgcag atgacagaga aaaagtaaaa ttattggaag gtaaattatc tgaagaagaa
      661 cttggaaaaa tggtgcaaga aatcaatgaa gacagcaact attttcctga attgaaggta
      721 tatcaaaacg acgaagattt ctttaatgaa atgtttagta atagtccatg ggaagcagtg
      781 agtaaaacaa atttaggaga atggaacttt caagatgatc tagttgcatt tagcaatgat
      841 gggtatttac gatcatattc agaaagtgag tatagagctg aattaaaaac gtatgaaacg
      901 gatattctga tgagctattt aaatctatta gagagtggtc agggagagtt aagagataaa
      961 gttgaagaac tattaaaaga agagctatag ggattatttc aacataaaca caagcagaaa
     1021 actaccaaac aaaacgtacc atgcaaaaag ccggtttaac cggctttttg ctctattatg
     1081 agaggagatc aaaccatgag tgcaacaacc gatatggctt tagaagcagt aataaaagat
     1141 aatacaggaa atgtcgaaaa tgtagaagac tttagcgata ttgcaatcaa tctttcagca
     1201 tggatttcag ctgtaggaga agaaaatatc gaatttgaga tcaagaatat ggatgactta
     1261 aaagcaaaat ttccttttat cgcatcgaaa ctaagtcaat ttgccgagga gaaatcgtgg
     1321 aatgaattag ttagtttttc cgaaaaaatt tatgatatta cgcaagaaga tgagtgtgtt
     1381 tcatgggaaa tgaataatca atacaagaaa attgatgctt tcgtcaaatc acgtatagaa
     1441 ggaacagctt ttgagtttga aggtatagat accgcagata attggttaaa taaagaatcc
     1501 tttattaaaa gtatagcgga tgatcttgaa aaacaatggt ctaaggaaga ctatgcagag
     1561 tacctaaaaa cagaaagaga tacagaggtg aaagaaaaac tcactgaggt atgtttggat
     1621 gaacatattg atcccaatat cgtaaaacaa gaagactatg aagtggttat tgataaagtt
     1681 cctactgatt ttgaggaagt tgtaaagtat atagttgaag aagcattatc gcaatactct
     1741 tcagatgagg aagtggacga agaaactgtt cgtaccagag tttggcaact ggtttttgat
     1801 gacaaagatt ataaatattt gagtgatcaa aactcggtta attggagtgt acgattatta
     1861 attgatcaaa atgatattga ataaaataga gctgaaaagc tctatttttt ttgataaaaa
     1921 aactagctat ttttttaaaa ataatttggt tcaaaattta tttttttaac ttgataattt
     1981 tttgccatcc ttttttaaat tggctatata tcaaggttta tagtggggat aaataaaaat
     2041 acatttgaca aaatttacaa tatagtttat tatatattta tcttattatg ataacacaat
     2101 aaaaggaatg atggtatgaa aaagaaggag tttagtcgat cgttgttagg aattttagtt
     2161 ttagttgcgg ttggtcttag tggcaagggt tactttcaga ataacagcac tcaggaacat
     2221 gattttcaga tcaaaagtag tgttcaggta ggtaataaag cagacagtga atcaaaagaa
     2281 tttttaggga tacgttatga tccaaacaaa tatccaaata atatcgtaaa cattaatgat
     2341 gggaaatata cactgacaaa agaagaccat gaagcattat cagaaaaagg cagtcaaacc
     2401 ttttgggtaa attatgcaga tttagatcaa cttggaagag caggagatgt tgaagcgttg
     2461 atcacctatg atgctgtaaa ttctcactca tctaaaaatg tggaacgtcc atctttcaaa
     2521 agttctgtcc atattgcagg agaatacaag gatggagtat ttgatcaaga gagagatact
     2581 tgggtcggca aagattcaaa caatgaaatc actcagctag aaggatataa aggttatctc
     2641 tataacaaaa gtcattcatt agcatggtcg ctaggaggag atatggaggt acacaattta
     2701 acacttggaa cgagagcgca aaatgttggg aaaaatgatc agaatggagg gatggcttac
     2761 tcagaaacga ttgtacgtga tgcaatctat aaaaataaga atgaaaaggt attgtataaa
     2821 gttgatccaa tttataaagg gaatgaattg gttcctcgtg gttctcatgt cgaagctttc
     2881 tcagttaacg ataatggaaa aacggtaaac cttaatgttt atgtatttaa caatcaaaaa
     2941 ggaatcaaca tcaactatca aaatggtact tgggtaagag attagaaatt gagaaggtga
     3001 agaattggaa acaataagca tcaatggatt agataaatca gttgcaaatc gaataaaaaa
     3061 aggaaatggc tactatcttc tgaagaaggg taaatattca gttgctagag agttagaaac
     3121 aatgagtatc gtgtatgtag ctaagaatca ttttgacgta gaacaggcta tttttgatcc
     3181 agattctgaa atagggaaat gtgttgaatt atcagagaaa actgttagta caaaaggggc
     3241 gatgatcatt gagagagaaa tgttagacga aatcagagta atcattccta aagatacgca
     3301 gatgtgtgag gacagcataa gtgacgatga accaaaatca gaaagaaaat aaggagatat
     3361 tcagatgagc gaaaaatcca ttgaattaaa ggaatataag cgaagcgatg gagaaaaata
     3421 ttatcctgta gccagttttt ctaaatcagt atatctggtt ggacgtgcat tggtagttgg
     3481 tgcaattggc atatatattt tgcattgggt aacaaaggtt gtacaactgc ttgtagatac
     3541 cttagtaagt attcttgtct cgttaaatga tgttaacgga acagatgatt taggattaaa
     3601 agaaatgttt aaagagttac tcaatgcgtt ttttcccaat tatttaagtt attcatataa
     3661 tgtacaaagc ttttttgcaa agctacactt ggtagacatc tctattttag tattagaagc
     3721 aggaacggta ttctttgttg gatatttggt tactttgatg ttcaggcatc actatggaga
     3781 acaaagacca ttttttgatg atatggaaag tagaaggttg aaaagagaag ctctagaagc
     3841 tatggatgtg aagtatgatc caaacaagat tataggagat gaaacgaaca acaaaaaaag
     3901 aaaacgaaac aaaacgcaat tagaatccga tgtgctatca acgcttaaaa atatcaaaat
     3961 ttctgttcat acaagacgtg atctaagtgt caatgaagat attcgagaat atatcgtaaa
     4021 aatcccgcaa ccaaaaggga agaaagttag agaaacagtt ttacgacaaa ttcaagattt
     4081 agggtcgatc ctgacaagag caacaagcgg acgagccaaa tttggtgaaa aagaagatga
     4141 agtttcaggc gattatttca ttttccgagc atcagtaaat attgaaagag aacgtaagga
     4201 aagagtaagt accaaaaaca aagaaacgga aagagcaaca tcttcaatcg cagtttcaaa
     4261 ttcacaattt tcttttccaa tcaatctctt tgccgataat tccaaaaata tccaaaacca
     4321 aaccgaaaaa gctagaaaat atgccgaaac tcaaaagcaa tctttagaac tttatctatc
     4381 ttctgctgac gtacctgcaa gtctaacaga tatgcacgtt gggaatacat ctattgaata
     4441 tttgtttact ttatctcgta ccagaaaaat tcaactaagc tcaatgaaag aagaaattga
     4501 aacttacttg catatatcag gtgtaatcgt gcggatcaac gcagggcgta ttcaaattac
     4561 tttaccatta ggagaaaatg atcgtattcc aattgatgta ccatcaatga tcaatagcgt
     4621 gttttaaaag acatcacaaa tagaaacaga aaatacaaac taccagttca caaacagaaa
     4681 agtaccgaga aaagtgagga atagaaatgg ctatgaaatt attagagaaa gtcaaaaaga
     4741 gagccgaaaa agctgaatct attttgctag ataaagaacc taatataaag cttaatggaa
     4801 aattcagtgt caatatgttg aacacaaagg ctaaagaccc gacagatgct atttttggaa
     4861 ttaagcctga tggcacaatt gctcattttt gcatcagcga aatgccacac atgttggttg
     4921 caggaacgac aggatcaggg aaatcagtat tactgaatga aattcttgtt acagcaatgt
     4981 gtcactcaac gcctgatgaa ttaaaaatag gaattgtaga cccaaaaagg gttgaatttg
     5041 gaagatacaa aaaattgccg ttcatgttgg ctgatccaat tacagatatg gatgaagctt
     5101 atgatttttt tgaatatctt gtgatcttga tgcatgaacg ttacaaactg atggaaaaag
     5161 cagggttaca aaacatcacg ctatataacg aatatgccga aaaaaatgga ttagatagat
     5221 ttccctatgt gatcttatta gttgatgaat attcacagtt ggttggaaca cataaagaag
     5281 tagaaggttt gattgttcaa ttaggacaaa tggcaagggc agcaggtatt catgttatct
     5341 tagctacaca atctcctcga tcaacagttg tcactggtat tatcaaagcc aattttccgt
     5401 cacgtgtttg ccttatggta gcaagtgatt tagaaagtag gatcgtatta gatgaaggcg
     5461 gtggagaaag cctaagtcca aagggagata tgattattaa gctcgttaat ggaagtatgg
     5521 ttagagcgca gggagcttac atcagtaaca aagaaattga aacaattttc aatcacctta
     5581 gaaatactat gccagaacca gaatatgttg attaccgagc aatcgtagct gaatctcgtg
     5641 gtgagggtgg agaagacgag gattttggtg gaattggtaa agctcctgta agaaaatctg
     5701 ctaaaagaaa tttgaacaca cctttagcaa attcatcatc taaagagcca tcaattgaaa
     5761 agaagagtac gtttgctcta aaacttgaaa aaaacaaaga agaaaaagaa aaacaaggag
     5821 ctaaaacact agaaaaactt caagccgaaa aattagaaaa agttttaaaa gcattagata
     5881 aagctgattc tgaaccgcaa acctcaaaca acacgaataa tgaacagccg atttcatcta
     5941 aaactaaacc gaaaactact gatccgaaag tttcagtaaa cgtgaccatc acaaccgata
     6001 agtctgagaa gaaacaagta aacggaacga cttcacctat catgcaaaaa atgttgaaag
     6061 ccaaaaaaca ggtgcataaa tgatcattgt cagttctttt ttgaggtggc ttacgccata
     6121 taaatataca gatgaagtag ttgtgaaacg aacgaagaaa agagataaaa aagcggtact
     6181 aactcctaaa cagatgcttg atcatggcat tttaggggtg aaggttttag aagtgaataa
     6241 accgtttgaa tcaaatagtg caagagaaaa gttgcttgga gctgaagccg atgtcgcata
     6301 tttttaccat gacaaaagca agcatcaaat agctattaaa ttcaatccaa aatcggcatg
     6361 gcggtatcaa gcaagagatg acttgaaacg taggaaactt agaccaatgc tctatccaga
     6421 aggaaaagaa gcatttgata gaacccacat cgttcccatt ggttttcatg gtacagaatc
     6481 agataaccgc ctattagtag gttggaacag caatcaaaat agagttcaga tcaataaatt
     6541 tgagaagaaa gtaaggaaat tcaacgatcg aaaaacgatc ctatggtttg cagatatttg
     6601 taaacagccg gatggaacag caaagtggga agcggtgata tttgacgaaa atggtaaaaa
     6661 agtaatgtct gaaacatggc atgataaagc taaatttgtt tggcagtagc agtagaggtg
     6721 aaaaagattg ctagaacttt ttgaagcaaa tagaacaggc tttaatcaaa aattagaagt
     6781 gggagaaact atagaatata aagataaaaa ctatattatt atcggaatct ataacacgag
     6841 aattaacaga ataggtgatc cacgtataac cagtgacttg gtttgtcaat ctgttaacta
     6901 ttcacctgat ctgacaagca ggtataagaa atacgtgtta ttagaatata gacacaagat
     6961 tactgatgaa attggtgtaa acaacacacg aaacatattt aagatcggaa cagtgattcc
     7021 ttatagtaac aacgaagaaa aaatatttta tcagatttac ggaatagaaa agtttgaata
     7081 tgaacatgtc gatctgctag tgacgtacca aatgcgttta atagaacctt ggtcacaggc
     7141 agaaattgat aaagcagtaa aattgaaccg attatcaaaa tttaacgtat taggaaatgc
     7201 cttttaaaaa tcgcaataca caaacagaaa aataccaaaa aattagcaat gaacgtaggt
     7261 aagaacatga caataaataa taataaccct aaaaaatata atgctcttgt ccaatgctat
     7321 atcgaaaaca aaattacaat gcaagaagtc gctgatgaat taggcaaatc aaagagccta
     7381 gtatcacacg attttaagca caatgttttt ccgaacccga ttgagctgag gaaaaaattt
     7441 caaaagagag tttatcaaga aataggtaag tggattgatg aagggaatgc aattgaccct
     7501 ttaattgaaa aatatccttt tttaaaaagc aaaaataaaa ttcaaaggtt aattcgtcaa
     7561 ggcgatattc aagctaaaga acctgttttg acaaccaatg atttggctaa aataaatctt
     7621 cataaacaaa ttattgaaga ctttgaaaaa aatctttcaa caactgaaat tttaaaaaaa
     7681 tacaatattc cgagaggaaa gctaacatcg actcttttta gagaatatgg agaatcggca
     7741 gttactaaag caaaggaagt agcgaatgag agaaaaaaac aaaaatagta gtcgatacct
     7801 agtagcgact gtagtttttg tttgtgtatt gattttcctg tttgattttt tgcagattgg
     7861 aaaaagcata attggaaata cattgagtgc aaaggaaatg gttgctttag gtggtttgta
     7921 tgaaggacac ttgttcccga atatattcat ttctatcgtt tctcatatta acttgtacca
     7981 tttaatttct aatttattgt tcttagtagt tcttgaagaa gcactgaatg atcagtatga
     8041 gtggttaaca ttattgatcg ttttaatcat atcaggattt ctaggaagct tagttccttt
     8101 tctgctacac agcaacatcg taagtatggg gcttagtgga ggtatttttg gatggattgc
     8161 tttatttgcg attgcataca tccgttttga tagcaagaaa catttattac taagtgtttt
     8221 tgtattattg ataatcggca ttttactggt atctactttt gaatcaggcg agacaaacgt
     8281 cttgtcacat gtaatcggat ttgtctgtgg gatgatcttg ggatttattc taaagaacgt
     8341 ttcattgatc aaacaaaaga attaaaaggg agtgctaaaa atgccaaaaa aaaaagaaaa
     8401 gaaaattcca gtgtttttgt ctgtctcaga aatgttatca cttcaaatac tgttaagaaa
     8461 aaagattgaa acaatagacg aagaagttag aaataaaagg gctatgtacg aacaacgtga
     8521 agcgctaaga gaacgaatcc actattttga tgatatacac actaaaatct atgaagcacg
     8581 tacaaatcac gaagacgaaa gcaaattagg attagctcaa caacgaatat ttgaatggct
     8641 gaatacgaat actgagcaag aaccgttaag agcaattagt aatctttttg aatttggaac
     8701 gaagcattgg tttaacgctg tagaaaagga tgctgactta caagaagcgt tcgatacact
     8761 aagcgaggat agcaaatcca tgcgagactt attgatcact tatctaaatg agcatgaatt
     8821 tgaacagacg aaagttaaaa agaaaaataa aaaatagaaa gttgtgatgt atcatgctgt
     8881 ttaccttatt aggttattta tgtttagttc ttgctttttt tggagcaatt gctatatttg
     8941 tcggtcagtt tcaagcaaat aatccgataa tgcgtaacat aggcattgga attgttatca
     9001 tatccttgat tgtatttggt gccacaaaga tttttgggag taatgatcag cagtttggta
     9061 tttctggagt tgataatagt tatgctccag attcattggg aagagtagtg atagatggaa
     9121 cgtttgagaa tgtaacgagc tacaaaaatg tttcattgac agtgaaagaa gatgacaaga
     9181 aagcatcaga tgacgttctc gctacgatca agccgtctca atcacagttt gaagtgtttt
     9241 atacagtcaa agagaataac cctgtacgca agctaacatt agtttttaac gttgacggag
     9301 aagaatacta taaagatgta aaaatcaata aagcatcaac gaaaaagatt gtcgaatcag
     9361 cgaaaaaaga agtagctaca gaagaaagta caactgaatc aagttctact gtcgaagttg
     9421 aactaccaga taaaattgat tttgatcctg aaaaagtcaa atcgtttgaa tctgtttctg
     9481 gttctatggc agatcggttt aacactgaag ttttcaaagc tatcaaaact gttaaattgg
     9541 atactgaaaa gcaaaggatt aatgttacct tattcaatgc agttgaatca atggatcagg
     9601 ataaaaaagt tacggttgca agagaactag ggaaaagagt aattgggttg tatcgaggta
     9661 cggcaaaacc agattcagac ggttcagatt tgccagttca cgtagtttat gaagacggta
     9721 aagtttttgg agactctaca gcaaatgaag cactaacgat taaattagct aaatagccgg
     9781 aaaattataa taaacaagtg gtgtcacacc accatcgcat aggtgtgaca ccttggagat
     9841 gattactgat ggtagtaaat aaagaaaaag taagaaagta tgttacttta aaaatagctt
     9901 ctgttgaata tttagaaaag cgagctaaag aagaagaaaa aacaagaggt aagaaggtaa
     9961 cagtttcaga gttgttagaa gaattaattg agagtgacca aacaataaga cgtgcattta
    10021 ccaataggtc tgttaaaagt tagggtgata ctatctttaa tttcaaccaa atttctaata
    10081 tatgatgaat agtttccttg tacaaaatat ctaattaaga gataaaagtt aggaggattc
    10141 attcaaaatg attatccaaa agtttgactg gaaaatgaaa ttaggaatta gtcttgtaat
    10201 attgggattt attatttttt tggggataaa agcacaaaaa aatcaatcta cttttgaaac
    10261 tatgtcttct caaattattg aggaatgttc caaaatttat tattgtgaga acaaaactga
    10321 agaatctaat ggcttttatt atatcgatca accacatgat gttgttataa aaatggagtc
    10381 acatgaagta aacgaatgga ttcaaagaag tactaatgta tcaaaaaagt tagaaagata
    10441 ttctcaaacg tttttaagta aagtcatttt ttcaaattgc aaaaatgaag aaacgtctca
    10501 aagagtagct agttctttag aaaatttaaa attttctgtt tcaaaaaaag agaaatttgt
    10561 agatttacga aaataaacta tcgaaagtag tatgattatg atcttaaaat atcatcttaa
    10621 gagaagaatt acgattgttt ataggattta caaaatgaat aagtttgaag agtgatttgg
    10681 tcgcagacta gatcactctt tttctgtaaa aaaactttac acttattttt gagtgtgtta
    10741 agatttttta tatagagtga cggtttcttg tcagagtgaa tgataaccat caatattgga
    10801 ggaataataa gtggaaaaca ataatgatat tagatatttt agcattgggg aaagagttac
    10861 aacagtaagt gaattatcaa aggattatag agataaagaa ggaacgatta tttctcttaa
    10921 aaagaatcga agaggtgttt ggcaatatgt tgttgattta ggggatggcg ttacagttga
    10981 gtgtaaagat agtcaactga tcagaaatga tggcagtcat tccattgaaa aagcaattct
    11041 tgtattccca aaagtatcaa gcaagattat gaatgactgg ttgcttggaa aatttggtga
    11101 gtacaataaa aaagaaatcg cagaagtgat cggcaaatct tatccaacag tattagggta
    11161 tctaaaaaag ataacgaaca aagaagcgaa agttgaggat gttttaacag taagagaact
    11221 tcagtcatta atcgaatatc ggaataaaca aataacaaat cctgagtacc ttaaaaaaga
    11281 agatggtcta ttgaaacaat tggaacaggt aaataaaatc cggaaaatgc aaaaggtagc
    11341 tcctttaagc ctagataact ttaaaaaaat ctatttgaaa gagatcgaaa aagactaatg
    11401 tggagtgtca ctttaccgtt tcaacctgta tctgcaccaa gaccaagtgc aagaaggaat
    11461 gttgctgatg caactaatga cgtagcagag aaaaggattt caacctatta tgataaaaag
    11521 tatctcgatt atttgaaaaa aataaaaagc tttatcgaag atcaaggttt gttagatgat
    11581 gaattttact cgatcgtaaa tgatccgatg ggtgcaatta tggaagtgga cttttactat
    11641 cagataccaa aaagctataa aaaagtctat aacattatga agacgacagc gccagattta
    11701 gataatttag taaaaagtgc tatggatggt attttcaaca tctcagctat acgagatagc
    11761 catatcacag ggttaattgc ttttaaatac aatgtggcaa acgaaggaaa aacagttgtg
    11821 aggatcaagc gatattcaga atttgagtgg gatacttctg aaggattgaa cggagatatt
    11881 tgggaagcca catttgattt caaaccagta gcaacgccaa gaccaaagtc taaatttaga
    11941 ggaaatggga ttatcactta ctatccaaaa gagtataacg actatttgga aaatatcaag
    12001 aatacgctta aagagaaaga tttggtaaat gatcaactga aaaaggtaat gaatgctcct
    12061 atgggggtta tagctcagat agattatttt taccaagtga gaaaagataa gaaaaagctt
    12121 gattcactga tgcgtacttc acagccggat attgataact tagtcaaagg aacattggac
    12181 ggaattttta acaaggaaat tggcatcaaa gatagcaggg tggtaggttt aatagctttt
    12241 aaatacaata cttttgaaaa gtcgaaaaca aacgtgcgac tacaggagat gggctgatga
    12301 ataataacga aaaaattctt catgtacttg atagctttga aacaattcaa gaggaactaa
    12361 agaaatatcg agatgttttg gaacaaagat atgattttgt caatcagcaa aagagtaatc
    12421 acatggattt cattctcaat atgaatgact tgaagaagaa attagttgaa cgaaaagaac
    12481 aagaaaagtt gatcaaagct tactttgaac tcggtgaaaa agaagttaaa aaggcaatgg
    12541 aactgaatga agatcgcagg gttttagatc agctgttaga acaattattg gtgatgtttc
    12601 aaaaaggacg aatagatgag gatttaattg aagaagggtt gagaaagtat cctgcaaact
    12661 caggtatcgg aatagtgctg aaagcgattg atgaagaaga aattgaagat ttcataccgg
    12721 cagaggattt tgaatcggca atggaatata taaaatatta cagccaaggg atcactgctt
    12781 ttagagagtt tgatcctgaa gatgttattc atgacttgaa caacctaaaa gaatggtgtg
    12841 aaggttatga agttgatgat tccggtttgg actacttgat cagtattatg gaaatcgaag
    12901 aagagatgcc ggataagcct gatccaacag atattttaga attaatccat gatgctagaa
    12961 atccaattgc atacatttct agaggatata ctgtattgga atactataag ccttatatat
    13021 cagcgatgaa ccatttaaga cgtgtattaa gggaaaaacg tgaataccgc tcagtgttaa
    13081 atgcatcgaa tagattagaa aaagctgttt cagagctaga tgcttattat cgtgaacatt
    13141 acctgcaagc aggtggtatg ccaagaaata cgaaagcaaa tatcagtagg tatataaaag
    13201 aagctgagta ggcggagtta gcccataaaa tggcaaaagg taacctgaaa ggggcgtttt
    13261 gaatgaaaag ggatgttttt gaattgtacg tattagaacg tctagaagac ggcatgatgg
    13321 cacaagagat aattgatgaa atcgatgata tgatcagcag atttggtcaa ttggaattga
    13381 atgatggaaa caaaaattcg gtagtgctac aacaattttg gttaaataat ttttcgtata
    13441 aggattttat caaagaaaag gcaaatagat tgtactgact aaattcacga ggatcagtga
    13501 tgagagctta ttgctaagag cagttaggct cttttttgtt tgtgttaaat tattttattt
    13561 tacaatacac atttatacac aaaaaaatgt tgacggatag tacacaaatg tggtaggata
    13621 tatttgtaag caagttagat taacaagtta aaggaaggaa gagcagttat gaaaaaagaa
    13681 gaactaatag atatgtttca aattgttgaa cgagcaaata atatgggaat tatgtttttt
    13741 gatcgtattt ctttaaagat ggatttaagc gttgcccacc aagaatttaa tctacgatta
    13801 aaagctttat tgatttctga tgatgttaat ttcgctcatg atgttgtagg tatacagaac
    13861 catatagata gagaaaacaa acgtatgggt gatggattct taccaagata ttcaagttta
    13921 tgatttggcg aaagattcaa gagtatgtgt cattattgat aggtactctt gaattagaaa
    13981 ggaggaatga atttaagaca acaacgatta tgatcttaaa acaatcgttt atgtaattgg
    14041 caatcttacg aataattttc atgcaataat gacaataaaa aatgaaaagg ggtaaaatca
    14101 aatgaaaaaa tttttagtag gaattctatt cacaatgggg ctagtgctat tatcaccttc
    14161 agtttcagca gaaagctcta tttccatgta tcgtatgtat aatcccaata gcggagagca
    14221 tctatacact agaagcgttg gagaaagaga caatctgaaa cgttatggat ggcattacga
    14281 aggtgtcgcg tggcaagctc caacaagtgg ttccccagtt tatcgcttgt acaacagata
    14341 taacggtgaa catttttaca ccgttgatgc cggagaaaga gataagattt cgcacacagg
    14401 atggacgtac gaaggtgttg cgttctattc ttacaacgga gcaaatagag ttcctgtgac
    14461 acgcttgtac aacaaatacg tgaattggca ccattacacg ttagatcaaa atgaaaagaa
    14521 cgtaattagt aaacaaggtt ggaagattga aggtacgggt tggtatgcaa tgccaaacag
    14581 cgcaccaaat cctacacctg cacctgctcc aagtaatgag aaattgctaa atgttcctgt
    14641 catttggcaa ggtaatacga tgttgtgtga aggtgcttcc ctgttagagg gattgcacta
    14701 caaaggagta gcgacaaatc aaaacttaac cagttttgta aactatatgc caagagctaa
    14761 tgacaataat ccttatcatg gctattctgg cgaatggcgc tacaatgtga gtgggacgta
    14821 ccaaggcatt atgcctgatc cagtagttaa atgggcaaaa aatgttggtg gtaatgccgt
    14881 taatatcact ggttcaggaa cgaatgggat caagaatgaa attcgcaatg gtaatccggt
    14941 tgtaacttgg attacttata actatgcttc acctcaattc aaacaaatgc cttggggacg
    15001 tgcagtctgg aatggacatg tggttctcgt ggatggcttc aaaaatggtg cataccatgt
    15061 tgttgaccct gtatttggcg caaaatggat caatgcaagc acgtatgaga gagcatttaa
    15121 tgtgactgga atggcggttg ctgtacgtta attgatagaa ttaataaata aatgaaataa
    15181 gagcttaatt actgaaatgg taaatgggct cttatttttt agaaaaaact ttttttgtaa
    15241 tacgaatcta taacaaaaaa cgttgaatat agtacacaaa agtggtatat taaaaccgta
    15301 gagattatgc gaagaggtga ttttgatgaa ccaaagaaga agaaaaaggc tagaagaaat
    15361 aagcgagcga ctatccgagt taaaggatga gttagaagta attcaagaag aagaggaaga
    15421 gtacaaagaa aatatacctg aaaatctaca gggatcagaa agatatgaaa aagcagaaga
    15481 atccgttgat caacttgaag aagctgttga tggtttatcc gacatattag aattgattga
    15541 agatatagct agctagagat gattaatgaa gatttcagga gtgagcaaaa aatgattgat
    15601 atttcactga tagagaagct acttttttca aataagtaca cagccaaaga gctggctaca
    15661 agatggcaaa tatccgaatc agctattaaa gcctatcgtc aaaataaggg aagcgtagga
    15721 tggcgtgatt ggaaaggaat cagcattgga aaagcagaag acataatgaa acgtgaagga
    15781 tttcaagctg acaatgattc taaaaaagtt gaatagttta atagtcgcca agaaaagtac
    15841 cagggcgagg ggaaaggtag attacaaagg aaaacacttt taaataaact tcgcaaaagc
    15901 atcaggttat gagtggctag ggctatacga atgtgatgaa aacaaagggc tttaatgtca
    15961 aagaaaacgc aatagggggg gaataaaatt tttaaaaaag atatttatgt ttggtttaat
    16021 gggaaaaaat atatgactac taacaaaagg tggtcagagc tagtacaaaa tacagttaaa
    16081 aaagtcatgc cagatggata tcatttcaac tatatacaat gctcacaaac aactgaaagt
    16141 atttattttt caatattaaa tgagaataga ctctattatt tcagggtaag ttcacaccat
    16201 aggaaaaaga gagaagataa tgttacgaca tttttatcgg gaaaatacaa tggcttcaaa
    16261 gagttaagtg aagcaattgg tgagtatcta aaaagtaata gatttgtcag aatttctgac
    16321 aaacatatat tgatcttgtt actttatttg aataatagct tcaaattatt ttggagagac
    16381 gacactttct actatattga gaacggcatg gatatttatt ctggtatcaa cggcatatta
    16441 aaacattata ttaaggcgtt acttcgatat ggggttcttt atcaagaaga tttaagtaac
    16501 caaattaaga ttagtaattc tggaaagtca ttactggcaa aatataaaaa attgctttct
    16561 cctatctaca aaggaataga tatggaagct gtaaattctg aaactttatt taatttagtt
    16621 gttgatgaaa agtttgcgaa tgaatttttt aaaagtaaaa aaattgaaag actaaataac
    16681 ctgtttcctg aaaatgcaat aaaagcttat gtagataata ataaatcacg attatacaac
    16741 tggtttattc ccaaacggtt gcgaggaaaa atcaaaattg gcgatcaagt gaaagtaatt
    16801 aatctaaatc atactaagat cgttaccgta acggagttgt atatagctga aaaagaagag
    16861 attacaaaaa tgaaaccagt tattaaaata ctagataaca aagagtgagt ggaatatatg
    16921 gtagcaaaaa agaatactct aaaagaagct tctgaaaaat atcaagatat gaaaagacga
    16981 ggattaatcc caaaggacaa aaaaggtata tggagagaat ttgttaagga agtcagtagt
    17041 gatcgaagtg gcggtgactt tgttggacga tatacagcaa aaggttatag caggcgctta
    17101 aggatcactg ttgaatatgg aacgaatgaa tcagctacag agctgaagca aaggtactta
    17161 gatacacgaa gagaagcttc aaaaaaatat gatgatttca agagcagagg tctaattcct
    17221 aagaagaaaa aggggatttg gcgtgagttt ttcaaagaag aagatagcaa tcggaataag
    17281 ggagacttca ttggacgata cacagcaaaa ggctacacta agaatttagg gatcaaggtt
    17341 gaatacggaa ctggtgaaag ttcatgggaa ttgaggaatc gctatttaaa tatattgtta
    17401 gagaaatata aagcagaatt attggagttg ttcgatgtta atgattttat cgagatggct
    17461 gaaaagtatg gtcatgaaga aggttttatg atcaatgaca ttatggttat tgataatttt
    17521 acggatttat tagcacttgc gaaaaaagca agggatttaa gagaaacgca gatgtatcta
    17581 ttcagaaata aaaacggaag aaaccggaga actgaatcga gtgagattga agattactac
    17641 gatgccttta cacacgatct gggagccttt gtagacgaga taagtggtgt aatgaacaaa
    17701 tatagggtct agaataaaaa agccggagat gagcagataa atgggaaaca atagcatata
    17761 cacacctgaa atgcagaata aaattaaaag acttgtcgga ttagggtgga tgacaagagg
    17821 aaaattagat tcttttagta cgatcaaata tcttttgatg gacatttacc gtcagaacaa
    17881 gcaaacagga atacattttt tgggctatga taagccggaa gatgcacctt tctttgttcc
    17941 aagttcagcc tatagagcca gtacctccgg tagcatgtac aacttggatt tgttcgagga
    18001 aggagcaaag atgatcgaat atgatttcaa cgaagcctat tcgaggatca tgagaaatta
    18061 tcctttgccg tcaaacactt atttgaaagc aaaatataat acagatcaag ctattttgag
    18121 agcgataaat agaccaaaag taagaccgta tgaaaattta aaaacgtata tgttattttt
    18181 ggttaatttt gaaggaacag ccaaagaagg aacatatgct aatccaaaat cacagtcgat
    18241 gtttgcggat tatggtgaaa atcccaaaac agctaatgag cctatttggt tatcggagat
    18301 tgagctaaca cttatccttg attattatga tattaaagag atggaagtac tagatagcca
    18361 cacatttttg acgaaaaaag gattactcaa agaatatttt gaaagaatcg agccattaaa
    18421 aactgatcct gatacgttga agctttataa aaatcttaga acgaaagtat ttggtgctat
    18481 cggacaaaaa tcattaagag aaggagaaac aaataccttc gataaataca gtatgtataa
    18541 tcgagcgtat tcctccatgg tagcaggcgt atttagagat cgtatggtta gatatgaaca
    18601 gaaatacgtt catagcgcat atgggctatt aaaaattcgt actgatggat tgtatttcag
    18661 atccgaagta ccagagtttg aagagctgta taaacttgga gtagttaaga aaaaagttca
    18721 tacaatatcc aaagaagaag tcgaaaaagc tttagaattt aaaaacaaag gatttgaaaa
    18781 taagggctcc tttgggaact ctaaagatga agttaagggt gttgtgatag acgatgattt
    18841 cgagtttgat tttgactaaa tactctttgg aaaagatggt aggaggtaaa tatgaagaag
    18901 aaaattgtgg aaggaaatgt aggtgttatt attctaaaag attcaactga ctatgctaaa
    18961 tcaatggcac taaaagacga aaaagttgca cgcatggata aagatttagt tgaatttctg
    19021 atggatgata cagaaagatt aaaaggggat cagtttttga gtttaatgaa agacaaaggc
    19081 ttaaacacat atggtttatt tgattataag aaattagcta tagagtgggt aaaagaagga
    19141 gaaatatttt ttattgaaga atgggaaggt actgagaact tagttcttca tcagtatctt
    19201 ttgaaagctt aatctaagta aaacagttct gactagttat gcttgtcaga atttcaaagg
    19261 atgttttcca taaccatcat cattcaaaaa taaaggattt gaaaataagg gttcctttgg
    19321 gaaatctaaa gatgaagtta agggtgttgt gatagacgat gatttcaagt ttgattttga
    19381 ctagattttt ttgcaaggtt taagagtaaa caaggtaaga actaagatca tgaagattga
    19441 gagtaaagaa gatagaggac gaatgtatga gaacacacta tatttattta gaagataaaa
    19501 ttatagatga aattccggca aaaagtcgag aacaagctag aaaattattt attgaaaaat
    19561 acaaagagat caatggagat agtgaagttc caaaagtggt cgtaacagtt agaaaaaggc
    19621 aaacaaaaaa gagaagtacg tttcatgtca gccctgatat atataatctt atagagcgac
    19681 atagaagtga agataatatt tctatctctc aatatattgc tacagttatt gatgaattga
    19741 tttcaggaaa gtatgactat gcttttgatt ttgaggatga aaaggattac aaaagtgttg
    19801 ctgtctttca atctgatttt gagaagctaa aaaactatgc tggcaggaga aatgttaatt
    19861 ttgcaagact actagaagag ttctactccg aagagttggg agtaggaaat gaatacaaga
    19921 gagaaactta tgcgaaattg tctcataagt tatcccaagc gcttaaagaa gtgacagaag
    19981 ttaaagcgga actagatgct aaacttgaag aagtgaaaaa atatcaaaca gagaggaaga
    20041 acaacatgaa gaatcaagtg gaatttgacg aacagaaaga atttgaaaaa gcaagaaaac
    20101 gctataacaa aattatgaat gaacttagct atgaacactt aacagttcat gaagaattga
    20161 gtgagggtac agaaaaatat acgatcgcag atatggttaa tgaagtggaa actcttttag
    20221 cttctttctt tgaaaatggt cacgtaaacg atgaatggcg aaaagaagaa cctaaaaggg
    20281 ttaacgctta tattgctcga ttaaaaaggt ttattgataa atataaggag aatagtgaca
    20341 aattttctaa agataaataa ggagagagtg tgattacatt tgccagaaat cgaaggaata
    20401 ttttcgccaa aaataggtaa aaaacagtta gatttcagca gttttccgag cgtaaataga
    20461 gattataatc taggtgatga ggtacagata ctaaatattg atttcaaaga actagacttc
    20521 gctgttgctg tttccaacaa acccaaggca ttggaacgag tgaaaaaaat ataccaagag
    20581 aatgatcaaa atatcaaaaa tatgatcaaa aagtatagac gagataattc aaaagttgaa
    20641 gacgttttat tcaacggtac ggcaaaacat gatttgaatg tttatatggc attaggagtg
    20701 gctttgtact gtaattcaat taatcagaca gatattttgg ttaatatcgt gaggaatagc
    20761 tatcaaaaaa tattaaaaat aagtagcgaa acaaaccctt attatgcaat gaaatatctt
    20821 ggggataatt tattaaatga taaaaaattg atgcacaaca ctgaagagct gttatctaat
    20881 ttggctgcga ttaactttct attcaaaaca ggttctgaag attattttat agataacgta
    20941 ctaaaaggtg cttacggaac agcattttat ccgctttaca atatgaaa
//
LOCUS       DAZKKW010000045        20899 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.045, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000045 DAZKKW010000000
VERSION     DAZKKW010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20899
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.045"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            131..3421
                     /locus_tag="ACG8PO_001627"
     CDS             131..3421
                     /locus_tag="ACG8PO_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFQ0206939.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEVPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGPDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKDHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERLTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNQATGEVLENAKDLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     ASYETKEILHTVDYRKPNYSTVYVTPEGSDTGNGSRQNPLSLTQALRYAYAGQTIVLA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWVLR
                     GFDVTNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVVIK
                     NSVAYRNGWVPGVEGEGNGNGFKMGDSSLSGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDFHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTVSKDWIESLNTHSQEGQQPVTRYANGSIHVHGLMQIKPEN
                     RQTEKGRVDGLPLSVGATFDGETSPSSGYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVV
                     YVMKQRKK"
     gene            complement(3839..5746)
                     /locus_tag="ACG8PO_001628"
     CDS             complement(3839..5746)
                     /locus_tag="ACG8PO_001628"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13-type metalloendopeptidase"
                     /protein_id="HFQ0206940.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNEQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIQYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            5876..6751
                     /locus_tag="ACG8PO_001629"
     CDS             5876..6751
                     /locus_tag="ACG8PO_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HFQ0206941.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            6831..7805
                     /locus_tag="ACG8PO_001630"
     CDS             6831..7805
                     /locus_tag="ACG8PO_001630"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFQ0206942.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            7984..9129
                     /locus_tag="ACG8PO_001631"
     CDS             7984..9129
                     /locus_tag="ACG8PO_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HFQ0206943.1"
                     /translation="MNEVKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGR
                     HAHEKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIE
                     HPAVLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLM
                     PIKEIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKR
                     DGVNIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSSEEREARNNKYKGFQQLIL
                     DTLEANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNI
                     EPSHVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            9266..9610
                     /locus_tag="ACG8PO_001632"
     CDS             9266..9610
                     /locus_tag="ACG8PO_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HFQ0206944.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            9881..11167
                     /gene="efmA"
                     /locus_tag="ACG8PO_001633"
     CDS             9881..11167
                     /gene="efmA"
                     /locus_tag="ACG8PO_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HFQ0206945.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(11305..11904)
                     /locus_tag="ACG8PO_001634"
     CDS             complement(11305..11904)
                     /locus_tag="ACG8PO_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFQ0206946.1"
                     /translation="MNYFTSDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELITQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            12308..13429
                     /gene="mnmA"
                     /locus_tag="ACG8PO_001635"
     CDS             12308..13429
                     /gene="mnmA"
                     /locus_tag="ACG8PO_001635"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFQ0206947.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGDMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            13675..15096
                     /gene="arcD"
                     /locus_tag="ACG8PO_001636"
     CDS             13675..15096
                     /gene="arcD"
                     /locus_tag="ACG8PO_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043858 - arginine:ornithine antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HFQ0206948.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            15110..16228
                     /locus_tag="ACG8PO_001637"
     CDS             15110..16228
                     /locus_tag="ACG8PO_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HFQ0206949.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SEFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVNPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILER
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(16288..17580)
                     /locus_tag="ACG8PO_001638"
     CDS             complement(16288..17580)
                     /locus_tag="ACG8PO_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HFQ0206950.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMSFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSISSGHSPSHRQ"
     gene            17730..18290
                     /locus_tag="ACG8PO_001639"
     CDS             17730..18290
                     /locus_tag="ACG8PO_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0206951.1"
                     /translation="MKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDIKAPSLY
                     NHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSNQAVYEL
                     LISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYLSLRFLG
                     YFTKEPTIDPEDSYFWMINDFIATLP"
     gene            18419..18796
                     /locus_tag="ACG8PO_001640"
     CDS             18419..18796
                     /locus_tag="ACG8PO_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HFQ0206952.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEAEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESGKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            18729..20801
                     /locus_tag="ACG8PO_001641"
     CDS             18729..20801
                     /locus_tag="ACG8PO_001641"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_function: GO:0098847 - sequence-specific single
                     stranded DNA binding [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0051304 - chromosome separation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFQ0206953.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLNLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEEQKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERIPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKMLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
BASE COUNT         6774 a         3780 c         4463 g         5882 t
ORIGIN      
        1 caacaactta agttatattt tttcttttta taaaatacac ttattataat tttgtttttt
       61 attgacagta aaaataaatt aggtaatact ttaagtaaac gctttctaat ttgttacggg
      121 aggaatacac ttgttaaaaa aacgtataaa atacagtgcg ctaatgatga atctccttat
      181 gctcagtacc ccggttttaa tttctgtcgt tgaagttgct gctaacgaac aacagtctgt
      241 gaacgaagaa aatgcacacg tgaacattga agggatgtca acaatggagg aagtaccaag
      301 aactagtgaa gatatggata cggaagaagg aaatgatcct atgatgaaag aatcagaaaa
      361 tgaaaagaca aaaaagcaat ctgaagaagc aaatgaagag tggaaagggg ttgttttcgg
      421 agaaagcaca agtgctagta ggtatgccat tgaaccatta gaagacggcg tgcggctctc
      481 ttccacgaat aacggcggaa aattccagtc aagcggacca gatggaatga cctattatta
      541 tcaagcaatc cccaaagaca tcaactttag gatgcgggcg acgatcgaaa tagaatcttg
      601 gacctatacg aatgcccaag aaggatttgc tttaatggta agagacgcag taccaaaaga
      661 tcatttgttt gggcaggcat tttattcaaa ttcatttgcc atactaggca gtcggatcga
      721 atatgtctgg gactcggata gacaagaagt ggtcaacacg agcggcagca aatatacgat
      781 gcgacttgga ttagggacac gggcaatcac aggaatatca tctgaagatc cgcaagctgc
      841 gccagctcct gggagtgtct cagtggaaac aacaccttta gagacttcag cagggttctt
      901 ggaaaaagaa acaggttctt ataatatatt cggaaacggt catgggaatc tgtttccggc
      961 cacgaactcg attactaaac tggacgtaga gatgaaaaaa acgaatactg gtttagaggc
     1021 atattattac gatcctgaaa caggagaaga aatggcttct gacatcatgt atgactggga
     1081 aaaattatac gcttcagatg aatcggttat ttatgcagga tttgctgcag caaggaatat
     1141 gacgatcaag gtagaaggaa tcgaagttgc ttactcggat cctgcgacag atcctcctgg
     1201 gcaagaaaga cttactcgct tgattgatcc gatttacact gtgacctcta gtaatcattc
     1261 aggaaaccaa gaccatacta tgtcctttag agcaaatgct gacggattat taacaatcaa
     1321 aaatcaagca acaggagagg tacttgagaa cgcaaaagac ttggcaatca ctgcaaatcg
     1381 ggaatttgat tatcagacaa aactgatgga aggaacgaac gaatttactt tcagttttac
     1441 accagataaa aactatccgc cagaagaata cgtagagatg gctagttatg aaacaaaaga
     1501 gattctgcat acagttgact accgtaaacc taactattcg acagtttatg taacaccgga
     1561 agggtcagat acaggaaacg gaagcagaca aaatccgtta tctctgacgc aagcattgag
     1621 atacgcatat gctgggcaga caatcgtcct ggctcctgga acttattcct ttgaaagagg
     1681 attgacgatt ccaaaaggag ttaacgggac aagagaaaac ccgattcaat tgattgcaga
     1741 gaaaaatcca gaaaacaagc gacctgtact tgattttaaa ggaactggaa atgggatgac
     1801 actgtttagt cattactggg ttttacgtgg gtttgacgtt acaaatagtg ccgctatgca
     1861 aaaaggattg cagatttctg gaaaccataa tttgattgaa gagatccatg cttatcggaa
     1921 tggaaataca ggaatccaaa tcagtggtag ctcaaatgat cctttcgaag catggccagc
     1981 gcataactta gtcaagaact gtacatcttt tgaaaatgcc gatccaggat ttgaagatgc
     2041 cgatggattt gcggccaaac tgacaatagg agaagggaat gtatttgacg gttgtatcgc
     2101 ttatcataat gcagatgatg gttgggatct ttttgctaaa aatgaaacag gttcaatcgg
     2161 gaaagtagtc atcaaaaact cagttgctta tcgaaacggt tgggtaccgg gagttgaagg
     2221 agaagggaac ggtaatggat tcaaaatggg tgattcctct ttatcgggcc ctcatgaatt
     2281 gatcaatagt ttgtctttcg aaaatcttgc aaaaggaatc gattccaata gcggaacaga
     2341 tattatcgtc aatagcagta caagttttaa taacggttca tataatgtgg ctctttatac
     2401 ctcttcggca ggaaacacag acttccatgc ttctggtatc ttatctttcc gaacagaaaa
     2461 tcttgagatg agagagcaga tccgcccatt gaatcaaaac gaagatcaaa tctatcactc
     2521 gttgaactac tattggaatg aacgagagaa acaatcagaa aatactcttg gacataccgt
     2581 atcaaaagat tggatcgaat ctctaaatac acattcgcaa gaagggcagc agcctgttac
     2641 tcgctatgcg aatggaagca tccatgtcca tggattgatg caaatcaaac cggagaatcg
     2701 tcaaacagaa aagggaagag tagatggtct tccactatct gtaggtgcta cttttgacgg
     2761 agaaacctca ccgtcttcgg ggtatccaga atatgcaatc gtacaagaac caaatcagac
     2821 aaaaccaaat gaacaaaata atccgattat tatacaagaa gcaaatggtg tggcggagaa
     2881 tgcagaagga aatatcattg aactgaagcc ttttacagtt tttgtgtcaa ctttcacacc
     2941 aaatcaagga acgcctgtct ttgaagatat gcgttatttc ccagaaaaga tccaaatcgt
     3001 tgaaacacag tcaagtgctc aattttctaa tcataccgct attttttcag caccttcaaa
     3061 aggagaatat acgctagaag tcacttatcg ttcggaaata tttgatgggg aaaaatggtc
     3121 tgctcataca gaatctgtta cccagcaaaa agcgatcact gtgcgagaaa ctgccgatgt
     3181 gcccccggga aatgacaaac cagaaagaga aaatccagga aatgaatcac caaatggaaa
     3241 accaaatgga aaaccaaatg gaaaaccttc tcatccggat aggacagcaa aagaaagtga
     3301 aaaaaatggc actcaaggac tcccaaaaac gggagaattt aataacccgt tgctagcatt
     3361 agctggagga attttgttaa tcggtgtagt tgtttatgtc atgaaacagc gaaaaaaata
     3421 agtggaattt ctccttttat gatgtaaaac aattgactga ggaaagtaaa aatagaagga
     3481 aatgagacca atagatgtct cacttccttc tatcttttta tagatgaggt tctccttgct
     3541 gcttttgatt ggctgttctt attacttgat gcaggacagc tttttcacaa tctctccact
     3601 aaacggtgtt caatcagctg accctcggta ttagctcaac aaacggcaaa atatgaagaa
     3661 ctatttttcc gtttttttct ctaatactca ggtcaggacg ctgatttcac aacctctcga
     3721 taaacggtgt tcaaaagcca gtttctcagt agtaagtcaa atttatgcaa aaatgtggaa
     3781 agctattttc acaaaatttt tcttactact tgaaactaag cggctttttc acaacctctt
     3841 accagattcg cactcttttt tctggcgcta gatacatagc gtcttgttcc gtgataccaa
     3901 atgtttcata gaattcgtca aggttttttg gttgcacgtt tgctcggagt ttagcagggg
     3961 catggacatc gatttgcagt aaaagttgtt ggtattcttt ttttgctttt gtacgccaaa
     4021 tcgttgccca attgacaaaa aattcctcga tagaaagatc tgattccgtt tttgctgctt
     4081 ccaatgcaca gcttagaccg cctgcatcag caatattttc cgaaacggtt aattttccgt
     4141 ttacttttcc gtcagcaaaa ggcagaccat cgaattcttc gatcattgct tgtgctttgt
     4201 tttgaaattg tgtaagatct tcttctgtcc accagttgtt caagttgcca tattcgtcaa
     4261 ataaagcgcc gttattatca aatgcatggg aaatctcatg agcgataaca gctccgattc
     4321 caccataatt agcagagctt gattgttcta gactgtaaaa tggtgcttga aggatcgctg
     4381 ccggaaagac gatcacattc cgaaatggat gataataagc attgaccgtg tcagcactca
     4441 tttcccattc tgaacgatcc actggtttgt tccatttgct gaaacgatct tttaatgtga
     4501 gtttgctgaa aacaagagta tttgaaagca aagtaccacc ctgatctgca ggtactgttt
     4561 tgaactggtc gaataatgct gggatcttat cggggtagcc tacttggata cctaagttat
     4621 ttaattttat gacggctttt tcttttgttg catccccaag ccagtcattt tcttctagac
     4681 gttttttata aacggcgatc atttttttca ccatttgttc tacgtctttt ttagcagctt
     4741 cgccaaaata tttttttccg tagtaatcgc caactacttg atcgaattgt ccagaggcaa
     4801 ggtaatacgc tgcttttttc tgaggcattg cttcgtctgt tccagacaat acgcgagaat
     4861 agattccgct gacttggcgg aattcttctg atagataacc actaagagaa cgtactgttt
     4921 taatgatcat ccagcttttg aataacggaa aatgagcttc tgtcaggatg tcattcaaat
     4981 gatcgaagta cactggatca gttacgatca ctttatctgg agttgtccca atcaaacccg
     5041 tgacaagttt tttcaagtcc agtttatctg tgtattgaat aaatgtatca aaagattgcg
     5101 gattatacat tttgctgtaa tctgcgtttt cttctgcact ttttacatga ggagcaatca
     5161 atttatcaaa ttgcactgct tgttccgtga ttgcttctgc ctgacttttt tctttgccgg
     5221 caagctctaa taactgaaca gccatatcaa agaaaacgcc tattagttgt gcaccgttgg
     5281 gatggtctga tgcataatac gtcttatctg gaaggaataa agaaggcgga taagcaaaga
     5341 aggcgttcgt ttgcgcattc ttcatatctg catctacatc cagattaaat ggcaatggta
     5401 atgtatccaa cagccattct ggaaattgct cattcaattc gtcgaaagac tgcaatgatt
     5461 caactttttc gattataggt tgaagtggtt gagcatgcaa ctcatttctt cggtcataat
     5521 cgtttgccag ttgataatat gcaaggaaat gtttcatttt ttctgaagac actttttctg
     5581 gatgggcaag cagatcgtct gtttctccca tcaaaagttg gtcgatcccg tcaactaaat
     5641 cttggaaacc gcctgtagct ggtttatctg cagggatttt tgctgttttt tgccattctt
     5701 cattgactgc ttcatagaaa tcttgtttca atcgttcttt gttcattcat cgatcgctcc
     5761 ttttttgttt cgtgtgccca ttatcttaca aaatgaagga attaacaaat agaacataaa
     5821 aaattgaaaa ttcgaacaat caaactgatt tgtttttttc aatccttcac ttataatgaa
     5881 aaaaaggagg cgatcccaac tgaatcaagt agtggataaa cctttatatt tcaaagtaga
     5941 taaaaaaatt aaaaaattag ccagcactca gcagctgcag tcaagaaaaa gtaaattgct
     6001 ttttttagct ctggtttttg aagatcaaag ttatgtgatt atcgatcaat caggacatcc
     6061 ggtagaatat tctccagcaa aatatactta tcaagagggt ctttcttgta aaaaatggaa
     6121 gctgatcaat gaagagccaa tcgagttatc tagatggatc aacagaaaag aagatattcc
     6181 cgttttgatc gaagaaaaaa ggtcaggaaa ggaactcgca aattgctggg tgggattgcc
     6241 agaagaacgt tttttgaggt ataagaaatg ggctaccccc tcaggctatc tttgtggtac
     6301 ttacgcggca gcagttctcc ttgcctatta tcaagactat cgtaaggggt ggatgcttcc
     6361 aaatgagatt cgcaagaaaa atacggctga ttcgttggtt ttgacgaagg cattgcgtag
     6421 ccagatccaa ccactaggac ttcctacgat tccttttcag gttagtacag ggatttctag
     6481 ttttttaaag aagaatggca atcatgagcg tgcacgagca actcttctag gttcatggca
     6541 gcgagctaca aaacgtatac gtgaaggcaa acctgtgatg attggtattt tgaaagtact
     6601 tggcagtaca tatggcaatc attgggtgac agcgtacgct tattttgaaa ctgaaacagg
     6661 agaacgttat tacaaagtac acgacaactg gggcgattat cataaagtca ttcctgccag
     6721 ttggagtaac ggaacagttt cacttcctta agaatttgct gaaattaaca gaaaatcttg
     6781 gaaaaatatg ttaaactatc gaagaataga gaagttttgg ggaggactac atgagtaata
     6841 agtatcagga tgacacgttg aagattttta gtttgaatgg caaccgtcca ttagctgaaa
     6901 aaatcgcgaa agtgttcgga acagaattag gtaaaagcgt cgtgaagcaa tttagtgatg
     6961 gcgagatttc catcaatatc gaagaaagta ttcgagggga tcatgtgtat atcgttcaat
     7021 caacgaatgc acctgtcaat gactactata tggaactact gatcatgatc gatgcaatga
     7081 agcgagcaag tgcgaaaacg atcaatgtcg tattgcctta ctatgggtat gctaggcaag
     7141 accgcacggc gaaacctcat gaaccgatca ctgcgaaatt gatcgctaac ttgatcgaag
     7201 aagcaggggc aactcgagta ctgactttag atctgcacac ggttcaagta caaggattct
     7261 ttgatattcc tgtagataat ttatttacga tgccgttgtt tgctcattat taccgtcaat
     7321 taggtctgac gggagatgac atcgtagttg tttcgccaaa aaacagcggt gttcagcgtg
     7381 cacgtagtct ttcagaatat ttgaacagca cattagccat cgtagatcat gcagatgatg
     7441 cttcaactga gggtggttat gtgatcggtg atgttcaagg aaagacatgt atcatggtag
     7501 atgatatctt gaacacagga gcaacgcttg cacgagcagc aaatgtatta aaagaaaacg
     7561 gcgcaaaaga agtatatgcc tgtgcgtcac atggattatt gtccgatcat gcgaaaagta
     7621 ttttagacaa tgcaccaatc aaagatattt gtatcactga ttctgttttc acagaagaaa
     7681 atcgtcatcc agaaaactta acaatcgtta catgttcaga attaatggga gaagcggtca
     7741 aacgcatcca tgaaaataca ccgatgagtc cgttattccg tctggaagaa aaagaattcg
     7801 aataaacaaa aaaagaggga aagaagttgt gacacaagtg agcagcttca aggagctgcg
     7861 acaagacttt tgtcacagct ccttttttct atgcccatga aacgtcctct agacaaaaat
     7921 gagctcttct ttggtttgtt cgctatttta tgctagaatg tgcctagcaa taatttggaa
     7981 gggatgaatg aagtgaaaac tatttatctg gatcatgcgg caactacccc catgcatcca
     8041 caagtaattg aagcgatgac ggcaatcatg caagacacgt ttggaaatcc ttcaagcata
     8101 catggatttg gtcgccacgc gcatgaaaaa ttggaaacag caagacaaat cattgcagac
     8161 agtctgcaag caaaacctca cgagatcatc tttaatagtg gtggaacaga aggggataat
     8221 acagctatct tagaaacagc gttctcgcgt caaaaagaag gcagacatct aatcactaca
     8281 gctatcgaac atcctgctgt tcttaatacc atgaaatatt tagagacaaa ggggtttgac
     8341 gttacatatc tgcctgtaga tgaaaaaggg aatctgtcga tcgaagaggt caaaaaagcg
     8401 ttacgagaag atacgatctt ggtctctgtg atgtacggga acaatgaaat cggaaatttg
     8461 atgccaatca aagaaatcgg tgaactttta gcggatcacc cagcttattt ccatacagat
     8521 gcggtacaag cgtacggaaa ccaaacgatc catccagaag aattaggaat cgatttatta
     8581 agtatctctg ctcataaaat caatgggcca aaaggcgtcg gtttcttata taaacgagac
     8641 ggagtaaata tccctgcatt gcttttaggc ggggagcagg aagaaaagcg gcgtgcaggt
     8701 acagaaaatt tagcagggat ctggggtatg gcaaaagcag ttgaactgct gtcatctgaa
     8761 gaacgtgaag cacgtaacaa caaatataaa ggattccagc aattgattct tgatacatta
     8821 gaagcaaacg aaatccctta tcaagtaaat ggtgatttaa cgaataaact ccctcatgtg
     8881 ttgaatcttc gcttaattgg cgaagcaaat gatttgctac tgatgaagct tgatcttaat
     8941 ggggtcgcta tttctactgg ttcagcttgt acagcaggaa atatcgagcc ttcacacgta
     9001 ttagaagcaa tgtatggaga agatacaccg atactaaaag aatccatccg tatcagcttt
     9061 ggtttgggaa ataccgaaga cgacatccag tatttccttg atcaattagt tcgtgctgtc
     9121 aaaaaataac tagtgaagtc attcttatat gaacagatgt ctgagagaac gccttcattt
     9181 tttagggaac ttaactgaaa aacctagtaa aaatcaggat aaacttttat aataaagaga
     9241 aactaatttt atcgaggtga aaatcatggc atttgaaaca acagcaacgg tactgggcgc
     9301 accaattagc tatcgtttgc atcctgatgc aaaaagatat acattgcgcg ataatggatt
     9361 tacagaaaca aacggaggca attatcaatt gatccgacca ttagatgcca cgccgcaaag
     9421 caaagaagga ttcaaattaa aaatcactgt atcaaaagat attcaaacat tgaaaatgtc
     9481 gatcactaca gcaaatggat tgaaagcagt caatattttc aaagatccaa aacagaaaat
     9541 gagccaagat aaattttatt ttctgatgga tgggatgatt agccgtgggt tattcgaaaa
     9601 agtcgaatga tctgtggata taaaaagaca actagttttg aaacggccta ttgttagatg
     9661 actaactttg taaggacgac gcaaattagt tgtctttttt atttttaaat ttaacacatg
     9721 ttgttatttt taatattttg ctgatttatt gtttctaagt aaagaaattt aaattgaaac
     9781 gattgttatt tagggaaaaa ggtatttttt gaaaaaagaa tcccgtataa tgattgttgt
     9841 gcaaacgcta tacaaaatga aaaagaaaag agggatgttt atggaaaatg aacagtcggt
     9901 cgttttaacg aattggaaga gaaattattt gttcttttta tcgggacagt ttttgtcagg
     9961 aatcactagt atggtcgtcc aatatgcgat catctggtat ttgacaagag aaaccggttc
    10021 ggcgacaatt ttgagttttg ctactttact aggaatgatt cccatggttt tactaagccc
    10081 atttgtagga ccgctagtgg atcgctggga taaaaaagct cttttgattg tcacggatat
    10141 tattgtagcg attttcgcgc ttattttagc agtagtcgga acgatttcag aatccttccc
    10201 gatttggcta gtttttgtgt ctttgtttat gcgttcggtc gcacaaacat tccaaatgcc
    10261 aacgatccag tcgattatgc cgacaatcgt tccatcttct cacataacaa ggacgaatgg
    10321 acagttagga atggtccagt cagcgaattt catcattgcg ccagctctag gtgcggcttt
    10381 gttttcagtc gttcctgtca attaccttat tttattagat gtattggggg cagtttttgg
    10441 tgtcggctta ttgatttttg tgaagatccc aaaagtttct cctgaaatat tggaagttcc
    10501 gcttactatt tttaaagatg caaaatttgg gctgcagcaa ttgatggata acaaagggtt
    10561 atggtacata acgatcaatg gtgcatttgt gatgctgttg ttcatgccag caatcagtct
    10621 gtatccattg atgacgctag attactttgg tggttccgtt ggacaggcgg gagcagtgga
    10681 agtagtctac gcagtcggaa tgctcttagg cggtgcactg atcagtttta taggaacatg
    10741 gaaagaccga atgaaaccta ttattatagc ctacatcatt atgggcctga cgatcggtgc
    10801 aagcggattg gttccaaacg atagtcaagg gttcttgtac tttcttatct tgaatgcagg
    10861 agcaggttgt gcaacgccat atttcaatac actgctgatg gcaatgatcc agcagagtta
    10921 tgaatcgaat gttttaggtc gtgtcttagg gaacttcaac tctttgatga acctggcagg
    10981 tccaattgga ttattatttg caggaccatt agctgatcgt ttaggagtag aaaaaatgtt
    11041 cttgttttct ggaatcggga ttttattatg cggaatcgtt ttatttttaa cgtcagctgc
    11101 tcgaaattat gataaagaat tacagaaaaa attggtaaaa gaacaccatg agcaaaaaga
    11161 cgaatagtat ggaatggagt tgtgacagaa gtcttgttac aactccattt ctgatcctaa
    11221 tgttcctgtg gcaataagcc tactcaaatg aaaaatatga gtaattatct ttatttgttt
    11281 aatacttgat atagggcagt tttttcacga cctatcgtac ttcctctttg cttcatagat
    11341 ttctttgatt tgttgctcag ataacggagt gccaaatggt agtttttcct ttaagagttc
    11401 tctttccggt gcgtctacgc ctacattgac atcgttctcg ataggtacgc tgtaatgatg
    11461 gatatgtcca tgaagattgc ggatagtgtc gttaggcccg agcaacatcg gataatgagt
    11521 caagtaatat tgttgatggt cgaatttaac gatggcacct acatcataaa aataaaattt
    11581 gggacttccg tcagtaagcc taggatcatt tttttctaaa taacggaaaa aagcacgact
    11641 gtcatggttc cctttgatca agtggattgt tccttgcagt tgagtgatca gttccaaaat
    11701 ttctgcattt gctttttcat attgcggatg catagcaagg tcgcctagat gataaacatg
    11761 gtcggtttct tttattactg cattccagct agtgatcaac tgttggttca tttcatccac
    11821 atttgcaaag agccgcggag caaagtcgtt ttttcctaat aagcgttcat ggaaaaagtg
    11881 catatcactg gtaaagtaat tcatgtatct cttgcctccc ttttttctta gagtaaacaa
    11941 aatcacttat ctgagcaact cataacatct aaaaagaaaa ggttgtaaat gagacgtgca
    12001 gctttaagcg ataagaagca attttgaaaa atagtttttc acaatttaca aaattattcc
    12061 ttattgccga aaggctggcc ctttttatga gtatgccaat tttttatatc gaatgctcaa
    12121 aagatgagtg attttttggt tgactatcat gtttgtgtac tgaataggtc cagtagaaca
    12181 gtatttttac gacttgataa ttaagggatg aaattttcag aaatatgacg tataatgtaa
    12241 aatactgaaa agttgcgacc ttcaactcgt agatgtttca gaatctaatt gaaatgatgg
    12301 tgatatcatg acagacaaca gcaaaacacg cgttgtcgta gggatgagcg gcggtgtcga
    12361 ctcatctgta acagcacttt tgttaaagga acaaggatac gacgtgatcg gaatattcat
    12421 gaaaaattgg gatgacacgg acgaaaacgg tgtatgcacg gccacggaag attataagga
    12481 tgtagcgaaa gtagctgctc aaatcgggat tccttattac tctgtgaatt ttgaaaaaga
    12541 atattgggat cgtgtatttg aatatttcct agccgaatac cgcgcaggac gtacacctaa
    12601 cccagacgtg atgtgcaaca aagaaatcaa attcaaagca ttcttggatt atgcaatgga
    12661 tcttggcgcc gattatgtag ctactggaca ttatgcgcaa gtcactcgcg atgaaaacgg
    12721 cgtggtccac atgcttcgtg gagtagacaa taataaagat caaacttact tccttagcca
    12781 attatcacaa gaacagcttt caaaaacgat gttccctcta ggcggcatgg aaaaatcaga
    12841 agtacgggca attgctgaac gcgcaggcct tgcgactgct aagaaaaaag attcaacggg
    12901 aatctgcttt attggtgaaa agaacttcaa acaatttttg agcaactatc tgccagcaaa
    12961 aaaaggagat atggtcactc ttgatggtga agtcaaagga caacatgctg gcttgatgta
    13021 ttatacgatt ggtcaacgtc aaggactagg gatcggtggc ggaggagatt cgcaagaacc
    13081 ctggtttgtt gtaggaaaag atctagcgac aaacacatta tatgtggggc aagggttcca
    13141 tcatcctgca ttgtatgcca caagcttgga tgcaagtgaa atccacttta cgacgaatga
    13201 accaatgcca aaagaattca agtgtacagc gaaattccgt tatcgtcagc aagacgttcc
    13261 tgtgacagtt cgcttattgg atgataatcg tgcagaagtc gtattcgacg aacctgtacg
    13321 tgcaatcact cctggacaag cagttgtatt ttatgatgga atggaatgtc tgggtggtgg
    13381 tttgatcgac cacgcatatc aagaaacaaa agttttacaa tacgtttaag cgatattaaa
    13441 tgaatcaaaa cagctatggc taacaaaaga aacacctttt cttttggata gcatagctgt
    13501 ttttttgcaa taaaattaat ttttattcat tttatacttg aaatacttgt gtaaaaagtt
    13561 cataatatcc aataatatga ataaagttgc tttttgttaa gcaagagcag ttgcaaaata
    13621 gaagggaatc ttttgcaagt gatttgttca attttataag tgagggtgat ggaaatggaa
    13681 aagaaatcga cgggtattag caaatgggga ctggttgctc tggttgtcag ttcgtcaatc
    13741 ggaagcggtg tgtttgggat cacaagtgat ctggctagtt ctgcagctcc agggccagcg
    13801 atcctttcat ggataatcgt tgggattggt attttagctt tggtattatc gctgaatcat
    13861 ttaggagaaa aacgtccaga tctagatggt ggtattttcg gttacgcgga agcttcattt
    13921 ggtaaattag gtggcttcat cagtggttgg ggttactggt tgtctgcgtg gctgggaaat
    13981 gttgcctttg caacgatgct gatgagcgcc atgggagaat ttttcccgat attcaaaggt
    14041 ggacaaaatg ttccttctat tttatttgca agtattttta tttggggtct gactttatta
    14101 gtaaacaatg gagttgaagg agcgagcgtc atcaatacaa tcgtgacgat ctgtaaatta
    14161 gtgccattat tcctattttt aatatttgta tttatcgcat ttaaagtgaa tatattcact
    14221 gctgattttt ggggtaacgt atctgataat ctagtgaatg gaacagggca gcaaggaacg
    14281 atctggttgc agattaaagg ttgtttgatg gttatgatgt gggtctttgt agggatcgaa
    14341 ggcgcaagtg ttttagccaa ccgcgcgaag aaacgttcag aagcccagca agcatcgatc
    14401 attggtctgc tttgtttatt gtttatctat atccttgctt cgatcttgcc atacggcgtc
    14461 ttatctcaag aagagttagc aactatttca caacctgcta tggcaaatat tttaaaagga
    14521 atcgtgggaa attggggagc agcgctgatc aatatcggct tgattatttc gatcattggt
    14581 tcttggttgt cttggacgat gctcccagca gaaacaacga tgttgatggc tcgagacggt
    14641 ctgttgccga aaggttgggg aaaaacgaat aaaaaaaatg ccccaactta ttcgttagtt
    14701 gttactgcaa tcttgactaa tctattctta ttgactttct tagttactga ttatgcctat
    14761 caatttgcct attctttatg tacagcggca attttgattt gttatctact tgtcgggatc
    14821 tatcaagtcc agttttcttt aaaagaagga atcaaaaaac aagtagtgat cggtgggatt
    14881 gctgtagttt ttgaattgat gggcatcatt ttagcaggat tttcttatgt ccttttatgt
    14941 agtattgcct atcttcctgg tttttgcttc tactggatcg cttgtcgtga aaccaaacat
    15001 caaattacgg gaaaagaaaa agtaatgatt gggctgattt gtgtggccgc actgctttca
    15061 atcgggttgc ttgctgtagg ctggatcaag atataagggg gaaagaaaca tgaaaatcgc
    15121 aaatttcggt gtagaagaat ggctaaataa atgggaaaag aaagcggtat atgatattgc
    15181 acaaagctcg atcgaagcat tgacattaga cgaactcgtt ggcttagatg ggactagcgt
    15241 aagtgaattt ttcgaaaaac gcaaaaacga accattggac tatggctgga tcgaaggatc
    15301 agatgttttc aaacaagaag tcgcttctct ttatcaaacc gtcaatccgg aaaacatctt
    15361 acaaacgaat ggagcgactg gtgctaatct tttagctcta tacgcgttag tggaagcagg
    15421 agaccatgtt gtttcgatgc ttcctactta tcagcaatta tatgacatcc ccaaatcttt
    15481 aggtgccgct gttgactttg ttcatttgaa ggaagaagaa gattggcagt ttgatttaga
    15541 acagctagaa gccttagtca aacctgaaac aaaaatcatc tgtttgaata gtgcaaacaa
    15601 tcctacaggc acattgctag atcggaaaac tttagaaaaa atcgcagaaa ttgctcgtac
    15661 agtagatgca tatgtattga tagatgaagt atatgcgcct ttaacggata aaggtgaatt
    15721 cttgtcgatc gtagatgtct atgaaaaagg tatagcaacc aattccctat ccaaaaccta
    15781 ctccattcca ggtattcgta ttggctggac agctactgga cctgagttgg cagaagtttt
    15841 tcgaaagtat cgagactaca cgatgatttg cggcggagtc ctgtcagatg atcttgctgt
    15901 acatgcattg aagaataaag aaaaaatatt ggagcgaaac aaaaagatca ttacagagaa
    15961 ccttgtgatt ttaaaacaat gggtagccaa tgaaccgaaa gttgaacttg tcgctcccaa
    16021 ttacgtctct acttctttca ttaaattagc catcgaagaa gatgaccaga cattttgtat
    16081 caatttatta gaagaaacag gagtactatt agtaccagga agtgcctttg atttaccgaa
    16141 gcatgcccga ctaggttact gttgtaaaaa agaaaccttg gaaaagggat tattcctctt
    16201 atctgagttc ttgaaaaaac aggcctaaac aaaaacactg caaggacagt ctgtgacaac
    16261 tgttcaagca gtgtttttca tttacgctta ttgtctatga gaaggagagt gccctgaaga
    16321 tatggagact cctgaagctt tttcttctgc agtttcttgc tgatggattt cttgtgtatt
    16381 gtgtcgtaca agcaagaagt tgaccaaaac aagaagagaa gtcgaaagaa aaacgccacg
    16441 ataaccaaat gcactggaaa cactggagcc gatcataggc cctactacgt taccagttgc
    16501 ttggaaagat tggttataac tgaagatccg tccagcagct tgatgaggcg aatattttgt
    16561 gatcaatgct tggacagcag ggagcagaca agcatcagag ataccaatca aaaaacgtaa
    16621 ggcagctaat tgccaaacat tcgtgacgaa tgacattggg atataaacca gaatcgcaaa
    16681 acccaaacca atcgcaagta ttctttcact cccgatcttg tcacctaagc gaccaaatct
    16741 tggagcggct atcaaagtgg caatcccagg tatcgaagcg atgattccgc tgatcaacgt
    16801 aacattccca tgtccatgca gcaattggcg aatgtacaga ctgatgattg gactaatgga
    16861 attgttggat gcttggatga tcatcgttgt gacgaacata ccgatgacga cgtgtggata
    16921 tttcaagtct ttgaaaatct gtttggcaga aaccatgttt tcttttttca ctgggacaaa
    16981 tttttcatgg acgaaaaaca gactcaacaa gaagacgaaa aataagatga tccctgtaat
    17041 gaaaaaggta gggcgatagc caaacgctga agcagagatc ccgccaagaa gcggtccaag
    17101 taatgttcct gtaacagaac cagttgctaa ggttccaaga acttgaccac ttttttcttt
    17161 tggcgtccca gttgcgacaa gggcagtggc attactgata taaccagaaa aaatcccttg
    17221 aagcagacgc aatgcgacta attgatacac actagtcacg caacccatca aagaaatgac
    17281 gattgccatt cctaatgaag cacgcaacag catcaatttt cttccttttt ggtcggctaa
    17341 ttttccccac caaggagaga tcagcgtagt caccaagaaa gtagatgaaa aagtcaatcc
    17401 gctccaaaag ttcaattgtg acgtactata attacctaac gtatctatgt aaagtgacat
    17461 aaaaggcatg actaaactaa atccgatacc agccataaat gttccaaacc ataagacaaa
    17521 caagttttgt tcccaagttt ctcttttcct aaataatttt tcctttattc ttgcagccaa
    17581 ttagactcct tctttctgaa aattttcatc tacagatctt tattataaac tacaatatgt
    17641 agtttatcaa tgaaagtctc ttttttctct ttctttttcg tggtaaactg aaagagtgaa
    17701 ggatttattt tatgagagga aacatggaaa tgaaaaaaag aaatctgtct caagagaaaa
    17761 tcatcgactg ctttcgagaa ctagcagaag agatggatgt tcagcaaata acctttcagc
    17821 atttagccaa acgcttggat atcaaggctc cctcgctgta taaccatttc aaaaatattc
    17881 gtgatgtcaa aacagcgcta acagcgaaac ttctgcagga acttaatgac cagctgcgtc
    17941 gggctcttgt aggaaaaagt ggtcaggaag cactgcagat ctatgcagaa acttatcgat
    18001 cctttgcttt ttcaaatcaa gcagtgtatg agttactgat cagtgtaccg cataccaatg
    18061 aagctgtttt attagatgga atccatgaaa cgaatcagat cattttgcag atatttgagg
    18121 cttttcaatt aacgaaagaa gaaaaagttc atcgaagcag ggaacttcgt agtatgatcc
    18181 acggttatct ttccttacgt tttttaggat actttacaaa agaaccaaca atcgatccag
    18241 aagacagcta tttttggatg atcaatgact ttatcgctac tttgccttaa acaatcagat
    18301 tcatttgtaa caattttaag atgttcatta aatgaccctt ttataagaga aaattcttgc
    18361 aactttcttg aaaagtgact aagatgagtc tagaaaagct gaaagaagga gcacgagtat
    18421 gtatcaagta attaccatgt tcggggacaa tgagccttgg tggttttttg aagactggga
    18481 agaagatatt gaagcagaag aaacattcga gtctttcgaa gaagcacgac aagcgtacga
    18541 aaaacaatgg aacgaaatcc atcaaaatta tgaatatatc aatgcaaaaa gtaactttct
    18601 cagcgccttt tggaatgacg gagatgagcg ctggtgtgaa gagtgtgacg atgatttaca
    18661 gcaatacaaa ggacttgcgt tgttgaaaaa tcatcagccc atcacagtag aaagcggaaa
    18721 ggaattttat gaaacaacta attctagcgg aaaaaccaag cgttgcgaaa gacctaagca
    18781 aggtgcttgg tgctaatcaa aagcacaaaa attattatga aggacccaag gtgatcgtta
    18841 cttgggccct tggtcattta ttaggattga aaatgccgga agatctgaat aaggaatggc
    18901 agagctggca aatggacacg ttgccaatga tccctaaaaa actaggaatc aaaccacttc
    18961 ctaaaacagg acatcaatta aaagcaatca agcagctagc tcagcgaaaa gatgtctcag
    19021 aagcagtcat tgcgacagat gccggaagag aaggggaact tgttgctcgc tggatcttag
    19081 agtatgtgca tttcaataaa ccagtgaagc ggttatggat ctcttctcaa acagataaag
    19141 caatcaaaac tggattcaag caactaagac ctgcgaaaga atatgatgca ttgtatgaat
    19201 ctgctcttgc ccgggcaaaa gcggactggc tggtaggatt gaatgtgacc agagccttaa
    19261 ctgtgaagta cgcggataat ttatcagcag gccgtgtgca gacaccgaca ctagcattag
    19321 tgagagacca agaaaagaaa atcgagcagt tccgtccaca aacgtatttc acgatcacgt
    19381 tgcaagctgg aaaagaacaa gccaaaatgc ttcaaaaaaa tcaatttgct ttaaaaacac
    19441 aagaagaagc acaaaaactt gttcaattga tcagtacaag caaaggaact gtcacagaag
    19501 tccaagaaaa attaaaaacg gaatctgcgc cattgcctta tgatttaaca gagatccaaa
    19561 gagaagccaa tcaacgctat ggctattctg caaagaaaac attaaatctt gtacaaagtt
    19621 tgtatgagac ccataagatc gttacttatc cgcgtacaga cagcaaatat ctgacaaatg
    19681 acatgaaaag tacgatgaaa gaacgattgc aagcagtcag cgactttgct cctgaggtca
    19741 agcagtattt gaaaaacgga gccatcgtcc aacagacaaa agtattccaa gacagtaaag
    19801 tgaccgatca ccatgcactt ttgccaactg aaaacagagc acgttatgaa aaattaagta
    19861 atgaagagca aaaaatctat caaatgattg tctctcgttt tttaggactg tttgctcaac
    19921 cccataaagt cagccaaaca aaagttacgg ttgaatttgg taaagaacga ttcatttttc
    19981 ggcaaaatcg tgtcgtccaa gcaggatgga aaggcgaaac tgaaactgag acggaaacag
    20041 tcaaatggga aaaaggaatg agaatcaatc ccgatttcgc catcaaaaaa gaactatctg
    20101 caccgcctaa accattgtca gaagcaagct tgcttggtca aatggaaaaa tacagtctgg
    20161 gaaccccagc tacgcgtgca gagattatcg agaaactaat caaatctgaa ctgatggaac
    20221 gtatccctca aggactgcaa gtatctccta aaggtaagca attattagta ttggtaaatc
    20281 cctctttagt aacaccagat ttgacagaaa gctgggaaaa agaccttgaa gctattgctg
    20341 caggaaagaa acaagcagga accttcttga atggaatcga aaaagaaaca aaacgcttgg
    20401 tgaatgagat caaatcaagc aaacaagaat atcaagattt ctcgattacc caaaagaaat
    20461 gcccagaatg tggggccaat ctgcgcgaga aaaacacacg tgacggcaaa atctatgtct
    20521 gcaccaattc agattgcaac taccgcagaa gaaaagatcc aaaagtctct aatcatcgat
    20581 gcccgcaatg tcaccggaaa atggaaatca tcgaaggtaa aaacggtgct tatttccgtt
    20641 gtaaatacga tggaacgaca gaaaaaatgc ttgataaaaa agagcagaag aagaaaatga
    20701 ccaaacatga agaacgacgc ttgctgaaaa aatatgcaca agcagaagaa ccagaagaaa
    20761 gcccattagc agcagcacta aaagcggcaa tgaagaaata ataagatatg aaagaggttg
    20821 tgacagaagt ggtcagcttc aagaaataag aaggaattta cgaaaattgc ttctcaaatt
    20881 tttgtgaaat ttcggctta
//
LOCUS       DAZKKW010000046        20845 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.046, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000046 DAZKKW010000000
VERSION     DAZKKW010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20845
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.046"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            738..1541
                     /locus_tag="ACG8PO_001642"
     CDS             738..1541
                     /locus_tag="ACG8PO_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFQ0206954.1"
                     /translation="MSQEVTENVIDNDVDLVSDQTNAIVRYNNGLNTTALRGFKPVEM
                     DVFWAILSKMKRQGTAEVTFDFNVFRYLVKYDRREKDSFYSVLKALSDKLGTLTYRFE
                     DSDVYEQLWLFQKFRIDKQQETVTIKANEEFEFILNSIGKNFTRFELDNFTKLRSSYT
                     KELYRHLMQYRNKEWRNGYWVVKVEDFRRALSIPSSYRMTNIDKRILEQAKEEFLAPD
                     ENGIQVFESFDYEKIYAKKGRKVDRLRFTFSEPESNLPSISMHNWLEED"
     gene            2148..2603
                     /locus_tag="ACG8PO_001643"
     CDS             2148..2603
                     /locus_tag="ACG8PO_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0206955.1"
                     /translation="MDAINWHEFVNYMEKLLDDLKSFSIADAVKHGLPVSTTMKMRGL
                     KVPDEIIKLRTKTIIETAKIVDSMNLDLKGKKTTYEKDFVRFLKNVIGKYLGKKSFTE
                     LRDEEIVPTPMVSKIRKNKAEGNYYLGTIAKIVEYLENKEQKKGTKKGR"
     gene            2722..3780
                     /locus_tag="ACG8PO_001644"
     CDS             2722..3780
                     /locus_tag="ACG8PO_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliS"
                     /protein_id="HFQ0206956.1"
                     /translation="MEKDVNSASTKDQYNIIGGLLGDKDKRTRHGLRIMLATFLGVFL
                     LLTGLGSYIYQLGNYNYYLVNNTTPIGSSLTFARSGAEITISDVWTDKTRDVTVVKLG
                     YSGNARKLLSTQGKNYNLYMATKEKDRPKKLEMSYGMLSTDGDAFLFLKGKLDERAYQ
                     VFIANQVSLISGDDTADDDTKVNVNESDSVTKALSQYSMNDVDNEGVISFGKKAKPVN
                     TPDNINFRINPYSDTTNVYQGSFLTKDGDIDYSKVVAVTSKNNVVNKLEDQLNASNTN
                     IKKIEATKAEYLERLQQNPDDKDSQESIRQLDSRMETEIKTINDTQKLIDQYKNADFN
                     KDSFGDMKTTFKYLTLTD"
     gene            3822..5771
                     /locus_tag="ACG8PO_001645"
     CDS             3822..5771
                     /locus_tag="ACG8PO_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0206957.1"
                     /translation="MENSTEFFKNIQTEFNHALTLMKPPKHKLVGRDDELHELSLILN
                     RPETPQAILLGEAGTGKTALASEWAKRQRQEGKKVQVYKLSIGQLRVGGDDVLQTRLE
                     TIIPRLKEYERILKSEDKDNEFVLFIDEFHKVVSSFGSGNKIGGDSLKEPLANESLQL
                     ITATTRKEYENYIKRDDALGRRLQPLELNELKSDVVVDILRQWLNTSSVKDDPEASLK
                     LSKKIPDELLEEIVQANKLYREDLSEPAKSIAVMEDLIAKDRVDGNAYNRESLLQIFG
                     KYNIQLDSDFDIAKLEEELKSIKGQPVAVYTVTMLFKRMKLGFDRFKGRKQPKATALF
                     TGSTGVGKTAMAKAVARGLYGDENKVLLVNMPDYKTKDDAMRFRKLIGTHVRHDPSAI
                     IVCDEYEKAGVETLDAFLSILDEGRISFTERGWDDREVNDTVSLSNTILFATTNAGAE
                     MYDSMNRYSSLSRVANQENSNEYSTELKREYRKVALEIKQALIGEGLKPEVINRFGRI
                     VPFWALSEQTLLEIAHNEILKSAELVRKHLNVDLRLPPQKKHFYGQEFYADDLSMHLV
                     FDRASQEDSKSGGARNILHLVESEFEEAITEAIITYPNAKIFDVKLSKAGGFEGKGDT
                     DAEIIAKPSIAESKENKFVFSVGGV"
     gene            5774..9463
                     /locus_tag="ACG8PO_001646"
     CDS             5774..9463
                     /locus_tag="ACG8PO_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3659 domain-containing protein"
                     /protein_id="HFQ0206958.1"
                     /translation="MKFKKSLLQNRKIKRWVVRGLVIVGALAVTLGAPFGMNDPTSDD
                     QGNKQETVANTNVAYAESKPAEKGVWTLYARILMGDKEVNDSATKTGQMNLLGQGGIS
                     ADFPYSDISKQGNAIAGGNAGTQLASTLATYNYYGYIETVSGNAIASQASSFIGGIGR
                     TLGSFIGSISLILSNIAESINELLGKFFMSMNIFSLMGWGKGGSDVSNPVAKALTSLL
                     NNLGLNNQLFKAIASLSMLFFTAILGYQGMKLLSKEGVNSKGLSPVLEKWIVRIFVLF
                     VFLPVAGSLLGVYINGIQSAKEDGKLPTNVVGDYIINTRLWASTQNLAPSSDTGGNVP
                     NGKPTSEIKFVDTDYEPSKKSEMIRMINTSSYARQNNDLDSNSVGMALLKEWSSNDTF
                     NVNTYAGDVNVAENDAYMGRAPAVWTGYNLGDGKEGNAIKKPTNLNGYIWSANQNVTD
                     DNRKPDGKGTTNNFDATQPWGVANNATFSTQSVALMLQSALDTSGAKFYAYNIAPSGI
                     QGSAKNMSTVKTEWRTASLVGHGAMGKFGSWLAMNAESIVLAVLYLACAWAMFNVNIV
                     KAFKREFVRVWEVVLTGSLAAGAGVMAISLGVVTTSLIGLQLPIFLITFVRAFGDTAV
                     GFIPNTLLGDGVANIVRSAMVLGVGYLLVFKKGEKVNNNLINMLIGLPLEVAFGFDER
                     AKLLFGNRNDAVRVVDGVSKAIKKSQGAKNPYYDGGRGSVKDMVAGGLGAVGAQGIAD
                     RLNNMGDSNADAKTIADKVVPETPASVPFNGGGVGGTINNTSFKDLVRQDQLRNNSQQ
                     YSDHLGRMVDEKGNFVDENGEVLKDSKFGKDAKGNFIDAKGRLVDEDGNLIDTNGKPL
                     EDNEDGNIGMDSQGRLIDDKGRLVDDEGNLVDENGRKLLNGDFGQDVNGRLVDDKGRL
                     VDEDGNLVDEFGKKLGKHSNGYNVNGDTVDEDGNIIDSRDALVADRNKELEQQQHTPI
                     AVAIPDDESDEVILDQAHQGLREEAKDKVVSDQAQEELKNKSNGEDVSNQYHQTAGNV
                     PVEGQSNELHNNFDNVADRNDTVLKEKESLRQETDKDQSKLKKTTIPTPIGSTVPKVN
                     QANDSETNTGLGMAKDNQSIREPKKTTESSKSNKSKSTSNKTGKSKEPSKAKSAVEYL
                     SKTGKNVYTSADKALAKAPKGSLAGMTYEAKNEAIKLGKGTAKVAKVTAKGATKTGKA
                     VKKSTMSVKDYIAKYSEQKPTDKKS"
     gene            9874..10212
                     /locus_tag="ACG8PO_001647"
     CDS             9874..10212
                     /locus_tag="ACG8PO_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0206959.1"
                     /translation="MFDSIKNLVSGMTPEMKTLVQAILYLMATFFALKPGFTAMKHFG
                     DAKWGEGLKYVGAVVAIFIIPVITTVMLLTMGTKTGNDLNQKANMISALIPVVGIYLV
                     TKFPKEEQLG"
     gene            10339..10560
                     /locus_tag="ACG8PO_001648"
     CDS             10339..10560
                     /locus_tag="ACG8PO_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0206960.1"
                     /translation="MLVHANEVLTSGFKGTDLAKIMNMNVNQFYDYRNGNKKIEKARL
                     ETLIKFEKAYLYVLDKQKRTIDRKKGVLQ"
     gene            10560..12725
                     /locus_tag="ACG8PO_001649"
     CDS             10560..12725
                     /locus_tag="ACG8PO_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0206961.1"
                     /translation="MAKFKDITESINYVAETVPVVVLSLKPQLLEVYQKEEKEAKAKK
                     AKAKVWKEFDALLTKDLPKKNYVVIEDVQKLSSEEFPDEEDAILDSEKYVVLLKSSLN
                     YVVDLPEKFKEKLLAGVRFMDSNGQLLVIPDDPNMFEREVEEATDFYSAVDQLEVATK
                     GLERIAETFNSSDAVEFEAVAEAETEAVPDEEEALFEDNDEATEAGNQEASIEETQTE
                     TYQQPKIDEKPESQSDKENYVYEAEKIVSESDVPKNLDADLLEKDFEQIIANKFQPVV
                     LDEVSDPVDLNEEMRSIAELKPLLFLAKESMNARISIANNKINSELNKTKEQLLLKGK
                     QMLADELLRIEEATSLSGQNQFTNTLSVAKESYEAEASKIDDLVMEKSSIIRKDYDMA
                     KQAYLEAELKKLEQRYDAEHLPEVKQQEDEFRKATEESLDQQYQEAIESIKNDALKQN
                     QEAKEVVVERIINSLKPEIEKSLTEVQEKTLEIVEKTKAENEAEFHDTKEKTTDVVVK
                     YFANKEEVEKLVAKDTNELESKLVAQQNIIEDYKNRVKEFKDLADGYKLNSQKLTEQS
                     EALREDLSRARNDLSQMAEKTKSLVVTTENQTESKRDFTSKKMATIIAVSAMACGTIL
                     GSSYFISHNHQEAQAQTETSISRVENSLGAKTASTSTTDNNYEATPTTEFKVGEDVVI
                     SIDGKTTKAEVLSIDGDKAVVKAPDGNQYKIQAHLTEKN"
     gene            12737..12967
                     /locus_tag="ACG8PO_001650"
     CDS             12737..12967
                     /locus_tag="ACG8PO_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFQ0206962.1"
                     /translation="MREENIVYPVIENQSNTKSSQLLQEVQVVYPVNELPQTNETVSK
                     SISLIGLLIVATALTVGVYRKLKVKGSISDGR"
     gene            12957..13823
                     /locus_tag="ACG8PO_001651"
     CDS             12957..13823
                     /locus_tag="ACG8PO_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0206963.1"
                     /translation="MADNSNYGFKHNLHQKPSETIVRVDWLEKHVNSTYSVTAKRLIV
                     SAIVVAVVVLGSIVYLVSDTIKLRFIFIPTLLMIIGFLILISQYMFIDPSSGSLISGS
                     IRLFKSVVNTARVTMGKKKPFRGTEILKVRQDGTIMYRDGSVALMFNLDGATSLTAFP
                     EEIIAQEGKSIQYQNARDRSLAEIKITSSQPQNTERQIRNMKVQKRNAKSNKAVFDIV
                     DQQERFVSEYIDGVKTTVIQYLILKSQSQMVLDEGVERLENYTSRGLYYSVTQLSAKQ
                     TKEILSEIYALK"
     gene            13841..15799
                     /locus_tag="ACG8PO_001652"
     CDS             13841..15799
                     /locus_tag="ACG8PO_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0206964.1"
                     /translation="MELMSRSKLLNFRKKKHKKEQNADSSKAVNNTYVSDLTSLVGYQ
                     FTPSYIRTGNRWGTIVKLVNTYGMNRNYEFGWMVKAIPEVKVEGVKAYLIEADKPVSQ
                     KDQQEIMHENVGGSTNSYENNLNDKNSDAEINKTVMRIIDLNTASLLDSTANKIIDWK
                     LEILLVSDSPNKISEQLIKLQRLYDEKMYGLKLMSFGGDQRDLFDRLLSEPVGSVYEY
                     TSMSSDFAGNDHAIRKGLDDADGWAIGTMSTSYASGVAFMAMDRSFKGKVLVASHKDA
                     TIKGYSEEISASSLWGQLIANNAMANHKRTFHIVLNKFDYFGDPTGETFVAKPSLNSV
                     IEKINLAEEGLNSLELFGDRKDIGDLYRRNLDKLTQIFYLLADRELNPATRVLLKKEL
                     GNFYISQKLWDINYEERSKLLVNSSDTMPVLGDFISYLTNAETKAKKSGTEKEIDRII
                     VLKDTLRTALDSFSKMFNQRTTLKEIGKSDKLQFYYNLSDLRTEINVMEAQFLNTFDF
                     ITSQAKKGDIVMIHGFDRLSVETVGLIKKRLTMLEEDGVRLAFLFDTIGSGESKDGIE
                     TADIFNTDGKIYQSFESDFNYTIIGKMTSEELMKYEKKVAPARKGEDDQKLSVDLRQY
                     LTSGRSNKFQIRRPADLTNNFVIGEFII"
     gene            15887..16495
                     /locus_tag="ACG8PO_001653"
     CDS             15887..16495
                     /locus_tag="ACG8PO_001653"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0206965.1"
                     /translation="MKSNIKIIAGDKMKEKENLVWYFNRNPHMLITGRTATGKTYLLK
                     RMADYLSQVGIVSASFDKTEFEEILSSSRKEMEDRFEYIKENRFKMFDVSYSDLDMKP
                     YFVLIDELDSRLLLLEKENPLAYNYLLLIIKDIVLKGRVVGVFFIAVRQGKQPLLESI
                     EDNMLTRIFLGDSKGNGTICFDGNTKKFQTELVTEDFFSVDC"
     gene            16738..17232
                     /locus_tag="ACG8PO_001654"
     CDS             16738..17232
                     /locus_tag="ACG8PO_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0206966.1"
                     /translation="MNNLPEKNRVFRLNGDLSQFKLDENGEFFVWMRHEHCWCNPFEL
                     RKENEIRTKIANGEWDYLFRWSDESFIANNMDHAFVSFLDNTWGTWYDCYLKKLGFQN
                     IDIDAIECLKEVDNKYEEEPYYLLKQRLANDSVFQVEVWENEDGTYEGLAQEIPLSEK
                     KCSI"
     gene            17229..18329
                     /locus_tag="ACG8PO_001655"
     CDS             17229..18329
                     /locus_tag="ACG8PO_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0206967.1"
                     /translation="MKRVYQQLLWISYITLFSFIFLFGVSVIVGTIFIWLTDEDMSLK
                     WFMCLLSCIMLVLLCLEVREFLPSHWQEKRKELREEIEIKTSDWMDHTWLPLKKNIKQ
                     KEFWVLQLWALGKFCSYVALLIFDLIFYSIGIFGTAYYLLTDHNRLAVSDIFTVLMTL
                     IFVIQLSIFSQLLIRFIRQKITIDKLFRSLVDLLIISVILMTLTERIEQSGKTVEGLR
                     DFLTMFIQFVNGHMYLFVSGFILSMIGYVTTKLIKRSIEKEIDIDLIPTLSGYDYYVN
                     YGKFEELLHTNIVRTSIREFTNKEIKDSIHGEPTRKFIDRQKKVYNFYEVEAILRYGI
                     GKLWLEGRNIRQNKPYVFWKSGVLEQFPRRNR"
     gene            18350..18685
                     /locus_tag="ACG8PO_001656"
     CDS             18350..18685
                     /locus_tag="ACG8PO_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0206968.1"
                     /translation="MKQYLNEWKVIEGSLVAQRIKGLPDCLEKNHLFQIREMLKKEQF
                     DPDQFLVVEYPTIGVYCCNHINDEKYFIIQEYEGQLTPFYTTWEMSEDGINNFPCESI
                     EESISQTEC"
     gene            18718..19050
                     /locus_tag="ACG8PO_001657"
     CDS             18718..19050
                     /locus_tag="ACG8PO_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0206969.1"
                     /translation="MDTAILYINIIPSISFIITLTIFIRRKKFFKEKKLVCFLMKSGV
                     VFLFSWLLLILSTSSFLIILFATVGLIDFGVISGAVMWFWLTRKPKKRNKKNGLENKR
                     LFRLMTNH"
     gene            complement(19211..20806)
                     /locus_tag="ACG8PO_001658"
     CDS             complement(19211..20806)
                     /locus_tag="ACG8PO_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0206970.1"
                     /translation="MFILDDEINKVKNPRIVPLLEEVRSSFYQGNYRSAIAVNYSALV
                     LDLIDKLSDLANIYEDDTSKKILEEIAKLRKSDPNSPRWEMELLEKTYNSTELIDSYE
                     YEDLKYIKDQRNYSAHPVVTYNGDTWELREITKETCQDVIRKSYEIVFLKNPILGKKV
                     TNEIIQYASRIHSMSVTPEEFHSALKNSYLKKLSEKAKENLCKTAFKFVFKLPYGNEE
                     VDRDRVSTFRLLDALIFDNKEFYLKILKKEISNYRFTAESREEINKSLGENQQITYTK
                     GFFLLSFIASHPELQRDFSEEIIQNLKISIKDFEPKQPITTHIEDYYRLRSLAVLGNT
                     KEELQEHLNRLLDPILQQNMRVSSLATSTLETIYNQYLYFGEKEMFLDFMIEHLTDSP
                     AFRFSDKDMEILPWIYDKINQDQFNKLLYNLNRNNQFYYNGYFSSFISNLLDFYKQKF
                     HMDLRTHYNGLLYPNAVTMDQKFQLKDKELKKLYELAESKSDTYPEIINDLKLNLVNK
                     SEQYLGEKINSEEKLLDYIKQIK"
BASE COUNT         7455 a         3006 c         4162 g         6222 t
ORIGIN      
        1 tgtctttttt tgtatatatt cggaatacca catcgctttt tgtccatgtt tgcatcgctt
       61 tttgtccaca ttcacatcgc tttttgtcca tgttcgcatc gctttatgtc caccctaaaa
      121 tctatttaag attgatataa agcctttttg gaggttgcta aagttttaaa gattaaaata
      181 tatattataa atataaataa aaggggaagc tgaagcttcc tccttttcct ttttttcaat
      241 tattcaaaat tacacaagca aaataaattg ctagatagtg ttagtttttg ttttaaggtt
      301 tttaattttt gagattttga aataagagag gaggaatcct cctttattaa atatctgtta
      361 atatatctgt taaatatctg ttaatatatc tgttaggggg gtcttaaacc cttgggagag
      421 taagtattga aggggtatga aggtaacact tttggacagt aaaggtaaca aaagtggaca
      481 gtaagggtaa catttttgga cagtaaaggt aacaaaagtg gacatttgag gtatcaaatc
      541 aggataagac gttaccttcg gttttcttga tttggaaatc taaaaagtag aaaactagat
      601 tagtaacaaa tctttgttga ctgttacctt tagttgtgat ttaatttagg taacaagcag
      661 aaaaaaagcg tttcctttaa tttagtacca aattaacatg attaaaggga tgcaacaaga
      721 agattggaga gtaatcaatg tctcaagaag ttaccgaaaa cgttattgat aatgatgtag
      781 atttagtatc agatcaaaca aatgcaattg ttcgttataa caatggtttg aacacaacag
      841 ctttgcgagg atttaaacct gtcgaaatgg atgtgttttg ggctatttta tcaaaaatga
      901 aacgtcaagg aacagcagaa gtaacttttg atttcaacgt ctttcgttat ttggtcaaat
      961 acgacagaag agaaaaggac agtttttatt ctgtgttaaa agctttgagt gacaaactag
     1021 gtactttaac ctaccgcttt gaagattcag atgtctatga acagctgtgg ctttttcaaa
     1081 aattccgaat agataaacag caagaaacag ttacaatcaa ggcaaatgag gagtttgaat
     1141 ttatcttaaa ttcaattggc aaaaacttta ctcgttttga acttgataac tttacaaaac
     1201 taagaagtag ctacacaaaa gagctgtatc gtcatctcat gcaatataga aataaagagt
     1261 ggcgaaatgg ctattgggta gtcaaagttg aagattttag aagagcgtta agtattcctt
     1321 caagttacag aatgacaaat attgacaaaa gaatattaga gcaggcgaaa gaagaatttt
     1381 tagctcctga tgaaaatggt atacaggtat ttgaatcttt tgattatgaa aaaatctatg
     1441 cgaagaaagg aagaaaagtt gatcggctca gattcacttt tagtgaacca gaatcaaact
     1501 tgccatccat ttcaatgcat aattggttag aagaagactg aaaagaagca ggttgtaatt
     1561 ttaatacaac ctgcttcttt tgaaaaaagt atgggtaaca cttttggaca ataaatgcaa
     1621 gaagtttttt tgtgaccaaa ctttatatta gattactgtg attttgtgtg ttagaaaagc
     1681 cctgatagaa aagcaaattc aaacactttt taatatgtat gggtaacact tttggacatg
     1741 tcaataataa atatattttc tttcgtcttt tgcctaacta aaggtaacac ttttggacag
     1801 tatagttttg taaactaatt agatcgggtt taatgggata tatcttagca tttatgtaag
     1861 aatgaaggta acattaatgg acatgtcaac tgtttgaaca aaaaaatata gaatatttgg
     1921 atctgatttt tcatttttcc aaacgttaaa aaatagttag ttattttatt aatagatagt
     1981 catccttaat gtttgaaata gcagaaaata agatattgtt tgataaatgt tgttgttata
     2041 aggaaaactt gaatttgtta attgatttat ttatgttatt atgatactat aataacataa
     2101 atgtgttacg cccgaacctc attttacaaa aatgaagggg tgagacaatg gatgctatta
     2161 attggcatga atttgttaac tatatggaga aactactaga tgatctgaaa agttttagca
     2221 ttgctgatgc cgtaaaacat gggttgccag ttagtacaac gatgaaaatg cgagggttga
     2281 aggttccgga tgaaattatc aagctgagga ctaaaacaat cattgaaact gcaaaaattg
     2341 ttgacagtat gaaccttgat ttgaaaggca aaaaaacaac ttatgagaaa gactttgtac
     2401 gttttttaaa aaatgttatc ggaaaatatc tagggaaaaa gtcttttact gaattgagag
     2461 acgaagagat tgtaccaact ccaatggtta gcaaaattcg caaaaacaaa gccgagggaa
     2521 attactatct tggcacaata gcaaaaatcg ttgagtattt ggaaaataaa gagcaaaaga
     2581 aaggaacgaa gaaaggaaga tagttcgtgt gataaatatg aatagggaag aatgtaccga
     2641 aatgataaaa aaacccgaac ctagaattac cgaaaaactg actgattgct tgctctttta
     2701 cgaaaggggg caggcaaact aatggaaaaa gatgtcaatt cagcatccac taaagatcaa
     2761 tacaatatca ttggaggatt acttggtgat aaggataaga gaacacgtca tggattacgc
     2821 attatgcttg cgacatttct aggggtgttt cttttattaa caggcttggg tagctacatt
     2881 tatcaattag gaaactataa ttattatctt gtcaacaata caacaccaat cggctcatca
     2941 ttgacgtttg ctcgatcagg agcagagatc actatctcag atgtttggac agataaaact
     3001 agagatgtaa cggtggtcaa acttggttac tcaggaaacg caagaaaatt gctatctaca
     3061 caaggtaaaa attataactt gtatatggca acaaaagaaa aagatcgtcc taagaagctt
     3121 gaaatgagtt atgggatgtt aagtaccgat ggtgatgctt tcttgttcct aaaaggtaaa
     3181 ttagatgaaa gagcttatca agtttttatt gctaaccaag tatcactaat ttcaggtgat
     3241 gatacagctg atgatgatac caaagtcaat gtgaatgaat cagattcagt caccaaggca
     3301 ctttctcaat attctatgaa tgatgttgat aatgaaggag taatttcttt tgggaaaaaa
     3361 gcaaaaccag ttaatactcc tgataatatc aatttcagaa taaatccata ctcagataca
     3421 actaacgtat atcaaggaag cttccttacc aaagatggcg atattgatta ctcaaaagtt
     3481 gtagctgtga caagtaagaa taatgttgta aataagctag aagaccagtt aaatgcttca
     3541 aatactaaca tcaagaaaat cgaagccaca aaagctgagt atcttgaacg tttacaacaa
     3601 aatcctgacg ataaagattc gcaagaaagt attcgtcaac tagacagtcg aatggagacc
     3661 gaaattaaaa caatcaatga tacacaaaaa ttgattgatc agtacaaaaa tgctgacttt
     3721 aacaaagatt cttttggaga tatgaagaca acatttaagt acctaaccct gactgactag
     3781 aaacaaatta ttcttatttt agaggaagtg aacacaaaca gatggaaaat agtaccgagt
     3841 tctttaaaaa tattcaaacc gaattcaatc atgctctcac actaatgaaa ccaccgaaac
     3901 ataagttagt ggggcgtgat gacgaactcc atgagttgtc actgatctta aatagaccgg
     3961 aaacaccaca agctatcttg ttaggtgaag caggaacagg taaaacagca ctggcaagtg
     4021 aatgggctaa aagacagcga caagagggga agaaagtaca agtatataaa ttatcaatcg
     4081 gacaattgcg agttggagga gatgacgtcc tccaaacacg tttggaaact ataattccaa
     4141 gattgaagga atacgaacga attttaaaat ccgaggacaa agataatgaa tttgtcctct
     4201 ttatagatga atttcataaa gttgtcagtt catttgggag cggtaataaa attgggggag
     4261 attcgctgaa ggaaccttta gcaaatgaat cacttcagtt gataaccgct acaactagaa
     4321 aagaatatga aaactatatc aaacgagatg atgctttagg tagacgacta caaccgttag
     4381 aactcaacga attaaaaagt gatgtcgttg tcgatatact aagacaatgg ttgaatacat
     4441 catctgtcaa agatgatcct gaagcttcct tgaaattatc caaaaagatt cctgatgaat
     4501 tgcttgaaga aatcgtacaa gcaaacaagc tttatcgtga ggatttatcc gaaccggcta
     4561 aatcaattgc cgtaatggaa gatttgattg cgaaggatcg tgtagatggc aatgcgtata
     4621 atcgagaaag cctgttacaa atatttggaa aatataatat tcaactagat tctgattttg
     4681 atatagcaaa attggaagag gagctaaagt ccattaaagg gcaaccagta gcggtatata
     4741 cggtcacgat gttgtttaaa cgaatgaaac ttggatttga tcgttttaaa gggcgaaagc
     4801 aaccaaaagc aacggctttg tttacaggat caacaggggt cggtaaaact gcgatggcta
     4861 aagctgtagc aagagggcta tacggtgatg aaaataaagt ccttttagta aatatgccag
     4921 actataaaac taaagatgat gctatgcggt ttagaaagct tatcggaaca catgtccggc
     4981 atgatccatc agcaatcatt gtttgtgatg aatatgaaaa agcaggcgta gaaacacttg
     5041 atgccttttt atctattcta gatgaaggaa gaatcagttt tactgaaaga ggatgggatg
     5101 atcgagaagt taatgatacc gtctcacttt caaatacgat tttatttgcg acaacgaacg
     5161 caggggctga gatgtacgat tcgatgaacc gatacagctc actatctcgt gttgctaatc
     5221 aggaaaattc aaatgagtac agcactgagc ttaaaagaga gtatcgaaag gtagcattag
     5281 agattaaaca agctctaatt ggtgaaggtc taaaacccga agtcatcaac cgatttggac
     5341 ggattgttcc attttgggct ttatcggaac aaactctttt ggaaattgca cacaatgaaa
     5401 tattgaaaag tgctgaattg gtaagaaaac acttaaacgt agacttacgc cttccaccac
     5461 agaaaaaaca tttctatggt caagaatttt atgcagatga tctctccatg cacttagttt
     5521 ttgatagagc ttcacaagaa gattctaaat cagggggtgc aagaaatatc ttgcatctgg
     5581 tcgaatcaga atttgaagaa gcaattactg aagcaataat tacctatcct aacgccaaaa
     5641 tatttgacgt taaattgagc aaagcaggag ggtttgaagg gaaaggcgat acggatgcag
     5701 aaattatcgc taaaccttcc atcgctgaaa gtaaagaaaa caagtttgtg ttttcggttg
     5761 gaggggtgta gctatgaaat ttaaaaagtc tttacttcaa aatcgtaaaa ttaagcgatg
     5821 ggttgtaaga ggtttagtta tcgtgggggc acttgcagtt actttaggcg ctccatttgg
     5881 aatgaatgat ccaacgtctg atgatcaagg aaataaacaa gaaacagttg caaatacgaa
     5941 tgtggcttat gctgaaagta aaccggcaga aaaaggggta tggacactat atgctcgtat
     6001 cctgatggga gataaagagg tcaatgatag tgccactaaa actggtcaaa tgaatttact
     6061 tggtcaagga ggtattagtg ctgactttcc ttattctgat atttccaaac aaggtaatgc
     6121 aattgccggt ggtaacgcag gtacgcaatt agcatcaacc ttagcgactt ataattacta
     6181 tgggtatatc gaaacagttt caggaaatgc gattgcaagt caggcatcct cttttattgg
     6241 tggtatcggt agaacgcttg gcagttttat cggatcaatt agcttgatac tttctaatat
     6301 tgctgaatcc atcaatgaac tccttggaaa atttttcatg agtatgaata tattttcact
     6361 tatgggttgg ggtaaaggtg gatcggatgt ttctaatccg gtagctaagg cgttaactag
     6421 cttacttaat aatttgggat taaacaatca attatttaaa gcgatagcaa gcttatctat
     6481 gctctttttt acagcaatac taggctatca aggaatgaaa ctgttgtcga aagagggtgt
     6541 taactcaaag ggattatcac cagtacttga aaaatggatt gttcgtattt ttgtattgtt
     6601 tgtttttcta ccagttgcag gatcgttatt gggagtttat atcaatggta ttcagtcagc
     6661 aaaagaagat ggaaaattac caacaaatgt tgttggtgat tacatcatta atacaagact
     6721 ttgggcttct actcaaaatc ttgctccatc atccgatact ggtgggaatg tgccaaacgg
     6781 aaaaccaaca tctgaaatta aatttgttga cactgactat gaaccatcta aaaaatcaga
     6841 aatgatcaga atgatcaata ctagcagtta cgctagacaa aataatgatc tagattccaa
     6901 ttcagtcggc atggcattgt taaaagaatg gtcaagtaat gatacgttta atgtcaatac
     6961 ttatgccggt gatgtgaatg tagctgaaaa tgatgcttat atgggaagag cgcctgctgt
     7021 ttggacaggc tataatttag gggatggaaa agaaggaaat gcaattaaga aacctaccaa
     7081 tttaaatggt tatatttggt cagctaatca aaacgttaca gacgacaacc gcaagcctga
     7141 tggaaaagga acaacgaata actttgatgc tactcaacct tggggtgttg ctaacaatgc
     7201 aacgtttagc acacagtcgg tagcgttgat gctccaatca gcactggata catcaggggc
     7261 taagttttat gcgtataata tcgctccatc aggaattcaa ggtagtgcta agaatatgtc
     7321 aacagtaaag acagaatggc gtacagcttc tttagttggt catggagcta tgggtaaatt
     7381 cggctcatgg ttagcgatga acgctgaatc aatcgtatta gcagtgcttt atctagcttg
     7441 tgcttgggcg atgtttaacg ttaatattgt aaaagccttc aaacgtgagt ttgtgcgagt
     7501 ttgggaagta gtcttaacag ggtctcttgc cgcaggagca ggggttatgg caatctcatt
     7561 aggagtcgta accacatctc ttatcggatt acaattacct atttttctga tcacctttgt
     7621 tcgtgcattt ggagatacgg ctgtaggatt catccctaac acattacttg gtgatggtgt
     7681 agcaaatatt gtacgatccg ctatggtctt aggcgtagga taccttctag tctttaagaa
     7741 aggtgaaaag gttaataata atttgattaa tatgcttatt ggattaccac ttgaagtagc
     7801 gtttggtttt gacgaacgtg ctaagttatt atttggcaat agaaatgatg ctgttcgagt
     7861 tgtggatggc gtttctaaag caatcaaaaa aagccaaggg gctaaaaatc cttattatga
     7921 tggtggcaga ggatcagtaa aagatatggt cgcaggagga cttggagcag tcggagcgca
     7981 aggaattgct gatcgtttga acaatatggg agacagcaat gctgatgcta agacaatcgc
     8041 tgataaagtt gttcctgaaa cgcctgcttc agtaccattt aatggtggcg gagttggtgg
     8101 aacaatcaat aatacctctt tcaaagattt agttcgtcaa gatcaattac gtaataactc
     8161 gcaacaatat agcgatcatc taggacggat ggttgatgaa aaaggaaact ttgtcgatga
     8221 aaatggtgaa gtattaaaag acagtaaatt tggtaaagat gccaaaggaa actttattga
     8281 tgccaaaggg cgtttggtcg atgaagatgg aaatttgata gatacaaacg gtaaaccatt
     8341 ggaagacaat gaagatggaa acatcggtat ggatagccaa ggacgtttaa ttgatgacaa
     8401 agggcgcttg gtcgatgacg aaggaaacct tgttgatgaa aacggacgta aattacttaa
     8461 cggtgatttt gggcaggatg tcaatggtcg tctagttgat gataaaggac gattagttga
     8521 tgaagacgga aatctggtcg atgagtttgg taagaaactt ggaaaacaca gtaacggtta
     8581 taacgtaaat ggcgatacag tcgatgaaga tggaaacatc attgattcaa gagatgctct
     8641 tgtagcagat cgaaataaag aactcgaaca acaacagcat acaccaattg cggttgcaat
     8701 ccctgatgat gaaagcgatg aagttatttt agatcaagca catcaagggt taagagaaga
     8761 agcaaaggat aaagttgttt cagatcaagc acaagaagag ctaaaaaata aatcgaatgg
     8821 tgaagatgtt tcaaaccaat atcatcaaac ggctggaaat gtacctgttg aaggtcagtc
     8881 taatgagctt cacaacaact ttgataatgt tgctgatcgg aatgatacag tcttaaaaga
     8941 aaaagaatca ttaagacaag aaacagataa agaccaatca aaattgaaga aaacaactat
     9001 tcctactcct attggttcaa cagttccgaa ggtcaatcaa gctaacgatt ctgaaacaaa
     9061 cactgggtta ggaatggcga aagacaatca atccattaga gaacctaaaa agacgactga
     9121 aagtagcaag tcgaacaagt ctaaatctac ttcaaataag acagggaaat ctaaggaacc
     9181 atctaaagct aaatcagcag tagaatatct atctaaaact ggtaagaacg tgtatacttc
     9241 agcagataaa gctttagcaa aagcgccaaa aggaagctta gcaggaatga cctatgaagc
     9301 taagaacgaa gctatcaaat taggtaaagg aactgctaaa gttgccaaag taactgctaa
     9361 aggagcaact aaaactggta aagcagtgaa gaaatctacg atgtcggtga aagattatat
     9421 tgcaaaatat agcgaacaaa aaccaaccga taaaaagtct taagaagaat aaaaagaggg
     9481 agcattaata tgctccttct tttttgttct tctcttcaag gggaatgtca agaaaaactt
     9541 aattgtcttc aaaaatttct tttttatttg gtagtaaatc tatgaaatat ttgctttttt
     9601 agttatcggt taataagagg gcttatgcta gttagttaaa atcgggtaac tagtcattaa
     9661 atgctgataa ataagcaatt tttacgaaaa aaaaagaaaa aaatttgagg gaaaatacct
     9721 ttcaatctac aaaaatttat gttattatga taacataaag atgtaaatat cgaagcgtct
     9781 tttctgtatt ccgatcattg acaagtgaac agagaaaagt ttgcgatgat ataattatct
     9841 tgtgaaaaat aatcgaaagg aaggatttat aatatgtttg attctattaa gaatttggtt
     9901 tccggaatga cgccagaaat gaaaacgttg gttcaagcta ttctatattt aatggctaca
     9961 ttttttgcat tgaaaccagg attcactgct atgaaacatt tcggtgatgc aaaatgggga
    10021 gaaggcttaa aatatgttgg agccgttgtt gctattttta ttatccctgt aattacaact
    10081 gttatgttgt taacgatggg tactaaaaca ggtaatgact taaaccaaaa agctaatatg
    10141 atctcagctc ttattcctgt ggttggtatt tatctagtta caaagtttcc aaaagaagaa
    10201 caattaggtt aaaaggtaaa ttgagcaaat ttggaggata tgccgatttg ttcaatttac
    10261 tttttgctaa aaaactacac tcgaagcgta gtttttgaag tgagggaaga caatagatgt
    10321 aagatggaca gagaaaagat gttagttcac gcaaacgaag tattgacgag cggttttaaa
    10381 ggtaccgatt tggcgaagat aatgaatatg aacgtaaacc agttctatga ttatcgaaac
    10441 ggcaataaaa agattgaaaa agccagactt gaaacgctca tcaaatttga aaaagcgtat
    10501 ctatacgtgt tagacaaaca gaaaagaact attgaccgaa aaaaaggagt tttacaataa
    10561 tggcaaaatt caaagatatt accgaatcta ttaattatgt agcagaaaca gttcccgtag
    10621 ttgtactctc tttgaagcca caattactcg aagtgtacca aaaagaagaa aaagaagcga
    10681 aggcaaaaaa agctaaagca aaggtatgga aagaattcga tgcgttgctg accaaagatt
    10741 taccaaagaa gaattacgtg gtgattgagg acgtacaaaa gctcagcagt gaggaatttc
    10801 cggatgagga agatgcgatt ctagattccg aaaagtacgt tgttttatta aaatcaagtc
    10861 tgaattatgt tgttgattta ccggaaaaat tcaaagaaaa gctattagct ggtgtccgct
    10921 ttatggatag taatggtcaa ttattagtta ttcctgatga tcctaatatg tttgaaagag
    10981 aagttgaaga agctacagac ttctacagtg cagtagatca attagaggta gctactaaag
    11041 gcttagaaag aattgctgag accttcaact cttctgatgc ggttgaattt gaagctgtag
    11101 cagaagccga aacagaagcc gtaccagatg aagaagaagc tctttttgag gataatgacg
    11161 aagcaactga agcaggaaac caagaagcaa gcatagaaga aacacaaaca gaaacctacc
    11221 aacaaccaaa aattgatgaa aaacccgaat cccagagtga taaagaaaat tatgtttatg
    11281 aagctgaaaa aatcgtatct gaaagtgatg taccgaaaaa tttagatgct gatttgttag
    11341 aaaaagattt tgaacagatc atagccaata aatttcaacc tgtagtttta gatgaggtta
    11401 gtgatcctgt tgatttgaac gaagagatgc gatccattgc agagttaaaa ccattgctgt
    11461 ttttagctaa agagagcatg aacgctcgta tctctattgc taataataaa atcaacagtg
    11521 aattaaataa aacgaaagag caattgctac tcaaaggaaa gcaaatgtta gcggatgagc
    11581 ttttgcggat agaggaagca acatcactaa gtggtcaaaa tcagtttaca aatacgctat
    11641 cggtcgctaa agaaagctac gaagcagaag catctaaaat tgatgattta gtgatggaaa
    11701 aatccagtat cattcgtaaa gattatgata tggcgaaaca ggcttatttg gaagcagaac
    11761 taaagaaact agagcaacgg tacgatgctg aacatctgcc agaagttaaa caacaagaag
    11821 atgaatttag aaaagcaact gaagaaagtc ttgatcaaca ataccaagaa gcaattgaat
    11881 ctattaaaaa cgatgctttg aaacaaaatc aagaagcaaa agaagttgtg gtggaacgga
    11941 tcatcaattc attgaaaccg gaaattgaaa aatctttaac agaagtgcaa gaaaaaacgt
    12001 tagagattgt cgaaaaaaca aaggcagaaa atgaagctga gttccacgat acaaaagaga
    12061 aaacaacaga tgtggttgtc aaatattttg cgaacaaaga agaagttgaa aagctagtag
    12121 ctaaagatac gaatgagtta gaatccaaac ttgttgcaca gcaaaacatt attgaagatt
    12181 ataaaaatcg agtaaaagag ttcaaggatt tagcagatgg ctataaatta aatagccaaa
    12241 aattgactga acaaagtgaa gctttacgag aagatttatc tcgtgcaaga aacgacctat
    12301 cacaaatggc ggaaaaaaca aagtcactag tagttactac agaaaatcaa actgagtcta
    12361 aacgtgattt tacaagcaaa aagatggcga ctatcatcgc tgtatcagca atggcttgtg
    12421 gaacgatttt gggctcatcc tacttcatta gtcataatca ccaagaagcc caagctcaaa
    12481 cagaaacctc aatttcaaga gtggaaaatt cactaggagc aaagacagct tctacttcaa
    12541 caacagacaa caactatgaa gcaacaccaa cgacagaatt taaagtcgga gaagatgttg
    12601 tcatcagtat tgatgggaaa actactaagg ctgaagtact ttccattgac ggtgataagg
    12661 cagttgtgaa agctccagat gggaatcaat acaaaataca agctcactta actgaaaaga
    12721 attaaggagg gctaaaatgc gagaagaaaa tattgtttat ccagtaattg aaaatcaatc
    12781 aaatacaaaa tcttctcaac ttcttcaaga agtgcaagtc gtttatccgg ttaatgaact
    12841 accacaaacg aatgaaacag tgagcaagtc aatatcatta attggcttgc ttattgttgc
    12901 aacggcgcta actgttggtg tttatcgtaa gctgaaagtg aaaggaagta tttctgatgg
    12961 cagataactc aaactatgga tttaaacata atttgcatca aaaaccttct gaaacaatag
    13021 tcagagttga ttggcttgaa aaacatgtca acagcaccta ttctgttaca gcaaaaagac
    13081 taattgtttc agctattgtt gttgcagttg ttgttcttgg ttcaatcgtt tatttggttt
    13141 ccgatacgat taaattgaga tttattttta ttccaacgct attgatgata attggttttc
    13201 ttatactgat tagtcagtat atgtttattg atccatctag cggatcactg atctcaggct
    13261 ctattagact gtttaaatca gtagttaaca cagcaagagt aacgatgggg aaaaagaaac
    13321 catttcgagg aacggagatt ttgaaagtta gacaagatgg aacgattatg tatcgagatg
    13381 gtagtgtagc actaatgttt aatttggatg gtgctacctc gttaaccgcc ttcccagaag
    13441 aaatcatagc gcaagaaggc aaatcaattc aataccaaaa tgcaagggat cgttccttgg
    13501 ctgaaatcaa aattacttca tcacaacctc agaatacgga acgtcaaata cggaacatga
    13561 aagtacaaaa aagaaatgca aagagcaaca aagctgtgtt tgacatagta gatcaacaag
    13621 agaggtttgt atctgaatat attgatgggg tcaaaactac ggtgatccaa tatcttattt
    13681 tgaagtcaca atctcagatg gtattggatg aaggcgtaga acgtttagaa aattacacga
    13741 gtagagggct ttactatagc gttactcagt tgtcagctaa acaaaccaaa gaaatattat
    13801 cagaaattta tgcactgaaa taatagaggt gagaaattta atggaactaa tgagtagatc
    13861 gaagctactt aatttcagaa aaaagaaaca caaaaaagag caaaatgctg atagttctaa
    13921 agcggtgaat aatacttatg tcagtgattt aacatcattg gttggttatc aatttacacc
    13981 atcttacatt aggacaggta atcggtgggg aacgattgtt aaattagtga atacgtatgg
    14041 aatgaacaga aattatgaat ttggatggat ggttaaagct attcctgaag tgaaggttga
    14101 aggagtaaaa gcttacttaa tcgaagcgga taagccagtt tcgcaaaaag atcagcaaga
    14161 aatcatgcac gaaaatgtcg gtggatcaac gaacagttat gaaaataact tgaacgataa
    14221 aaattcagat gcagaaatca ataaaactgt tatgcgaatc attgatttga atacagcatc
    14281 tctgcttgac agtacggcaa acaaaattat tgattggaag ttagaaatcc tcttagtatc
    14341 cgattctcct aacaagatca gcgaacagtt gatcaaactg caacgcctgt atgatgaaaa
    14401 aatgtatgga ttaaaactta tgtcttttgg tggcgatcaa cgtgatctgt ttgataggct
    14461 tttaagtgag ccagtcggta gtgtttacga atacacatct atgagttctg attttgcagg
    14521 aaatgatcac gctatccgta aaggcttaga tgatgcagac ggatgggcta tcggaacgat
    14581 gtctacaagc tacgcttcag gtgtagcgtt tatggcaatg gatcgttctt ttaagggtaa
    14641 agtcttagta gcatcccaca aagatgcaac aatcaaaggc tatagcgaag aaatctcagc
    14701 atcatcactt tgggggcaat tgattgctaa taacgcaatg gcaaatcata aacggacgtt
    14761 tcacatcgta ttaaataaat ttgactactt tggtgatccg acaggggaaa catttgttgc
    14821 taagccatct ttaaatagtg tgatagagaa gattaatcta gctgaagaag gacttaattc
    14881 tttagagctt tttggtgatc gaaaggatat tggagattta tatagacgta atcttgataa
    14941 actaactcag attttctatc tgttagcgga cagagaatta aatcctgcaa caagagtatt
    15001 gctgaagaaa gagttgggga acttttatat tagtcagaag ctttgggata ttaactatga
    15061 agagcggtcg aaactgttag taaacagcag tgatacaatg ccggttcttg gggatttcat
    15121 cagctattta acaaatgctg agacaaaagc gaaaaaatcc ggtacagaaa aagaaattga
    15181 tcggattatt gtattaaaag atacattgcg aacagcattg gatagcttct ctaagatgtt
    15241 caatcagaga actacattga aagaaattgg caagagcgac aaattgcagt tttactacaa
    15301 tttgagcgat ttaagaacag aaatcaatgt catggaagcc caatttttaa atacctttga
    15361 cttcatcacg tcacaagcta aaaaaggcga tatcgttatg atccatgggt ttgatagatt
    15421 atcagttgaa accgttggat taattaaaaa gcgactaaca atgcttgaag aagatggagt
    15481 ccggcttgcg tttctctttg atactatcgg atcaggagaa tctaaagacg gtatcgaaac
    15541 agctgatatt ttcaatacag atggaaaaat ttatcaaagt tttgaaagtg attttaacta
    15601 caccatcatc gggaaaatga ccagtgaaga attgatgaag tatgaaaaga aggttgctcc
    15661 tgcaagaaaa ggagaagatg atcaaaaact atcagtggat ttgagacagt atttgacatc
    15721 aggtcgaagc aataaatttc aaatcagaag accagcggat ttgactaata attttgttat
    15781 tggagagttt attatttaag tcttatcctt gtttttgaat gtttaaggat aagaaacaat
    15841 atttatatac atgactgaga gaaagaatta tagtggcggt aaaataatga aatcaaatat
    15901 aaaaataata gcaggtgata aaatgaagga aaaagaaaat ttagtttggt attttaacag
    15961 aaatccgcat atgctgatta caggaagaac agctacagga aaaacttatc tgttgaaaag
    16021 aatggctgac tatttatcgc aagttggtat tgtttctgct tcttttgaca aaactgaatt
    16081 tgaagaaatt ttatcttcgt ctcgaaagga aatggaagat cggtttgagt atattaaaga
    16141 aaatcgtttt aaaatgtttg acgtaagtta ctctgattta gacatgaagc cttattttgt
    16201 tctgatagat gagctagatt caaggttgtt actcttagaa aaagaaaatc cattggctta
    16261 taattaccta ttactaataa taaaggatat tgtgttaaaa ggacgagtag taggtgtttt
    16321 ttttattgcc gttcgtcaag gaaagcaacc attgttagaa tctatagaag ataatatgtt
    16381 aacacgtatt tttttagggg attccaaagg aaatggaacg atttgttttg atggaaatac
    16441 aaagaaattc caaacagagc tagtaacaga agatttcttt tctgtagact gttagtatag
    16501 cggaggttaa ataatgaaac caaacataaa aattttagat tgaatatttc taggtaaaga
    16561 cacagaagta atcctaattc aacatgaaga aggatttgaa gttagtatag gtattcagaa
    16621 actccaaaaa cctcattatt gtaatcagtt gtataaaaac tttactgatg aagaaaaagc
    16681 gagagttttt tttaatgaag tcaaaggtat gagagaacag tatgaggtgg ttgaagcatg
    16741 aataatttac cagaaaagaa tcgtgtattt agattgaatg gtgatctcag ccaatttaaa
    16801 ttagatgaaa atggagaatt ttttgtttgg atgagacatg aacattgttg gtgtaatcct
    16861 tttgagttaa gaaaagaaaa cgaaattaga accaaaattg caaatggaga gtgggattac
    16921 ctcttcaggt ggtcagacga atcttttata gcaaacaaca tggatcatgc gtttgtttca
    16981 tttctggata atacatgggg tacttggtac gattgttacc taaaaaaatt gggatttcaa
    17041 aatattgata ttgatgctat tgagtgtctt aaagaggtcg ataataagta tgaagaagaa
    17101 ccatattatc tcttaaaaca aagattagcc aatgattcag tgtttcaagt agaagtttgg
    17161 gaaaacgaag atggaaccta tgaaggtcta gcgcaagaaa ttccattatc agaaaagaag
    17221 tgttcgattt gaaaagagta tatcaacaac ttttatggat tagttatatc actttattta
    17281 gctttatttt tctatttggt gtatccgtta ttgtcggaac aattttcatt tggcttaccg
    17341 atgaagatat gtcattgaag tggtttatgt gtctactatc gtgcattatg ttagttcttt
    17401 tatgtttaga agttcgtgaa tttttgcctt ctcattggca agaaaaaagg aaagagctta
    17461 gagaagaaat cgaaataaaa acgagcgact ggatggatca tacttggtta ccattgaaaa
    17521 aaaatattaa acaaaaagaa ttttgggttc tacaattatg ggcattagga aagttttgca
    17581 gttatgtggc tctacttatt ttcgatctaa tcttttactc tatagggata tttggaacgg
    17641 cttattactt attaactgat cacaataggc ttgcagtgtc agatattttc acagtgttaa
    17701 tgacattgat ttttgtcatt caattgtcta tttttagtca attacttatc cgatttatca
    17761 gacaaaaaat aacaatagac aaacttttta gaagtttagt cgatttactt attataagtg
    17821 tcattctaat gaccctaaca gaaaggatcg aacaatcagg aaaaacagta gagggattga
    17881 gggacttttt gactatgttc attcagtttg taaatgggca tatgtattta tttgtgtctg
    17941 gctttattct ttcgatgatc ggatatgtaa ctacgaaact tattaaacgc tcaattgaaa
    18001 aagaaataga tattgacctg attccgacac tttctggcta tgactattat gtcaattatg
    18061 gaaagtttga ggagttgctt catacgaata tcgttcgcac aagtattaga gaatttacaa
    18121 acaaggaaat aaaagattcc attcatggag agccaacaag gaagttcatt gaccgtcaaa
    18181 agaaggtcta caatttttat gaagtggaag cgatcctacg atatggtatc ggaaaattgt
    18241 ggctagaagg aagaaatatc cgtcaaaaca aaccatacgt tttttggaaa agtggagttt
    18301 tagaacaatt tcctaggaga aatagataaa ataaaaggag cgaatggtta tgaaacaata
    18361 tttaaatgaa tggaaagtga tagaggggtc attagtagca caaagaataa agggattacc
    18421 tgattgctta gagaagaatc atttgtttca aatacgagaa atgttaaaaa aagaacagtt
    18481 tgatccagac caatttttag tagtcgagta tccaactatt ggggtgtact gttgtaacca
    18541 tatcaatgat gaaaaatatt ttattattca ggaatatgaa ggacaattaa ctcctttcta
    18601 tactacatgg gaaatgagtg aggatgggat caataatttt ccatgtgaaa gcattgaaga
    18661 gtcaatatct caaaccgaat gctaattgat tataactata agaaaggtag tgagttaatg
    18721 gatacagcta tattgtatat taatataatt ccgtctatat cctttatcat tactcttaca
    18781 attttcatac gtagaaaaaa attttttaaa gagaagaagc ttgtatgctt tttgatgaaa
    18841 agtggagtag tatttctttt ttcttggtta ttgctcattt tatcaacttc aagtttttta
    18901 attatattat ttgcgactgt gggtcttata gactttggtg taatatcagg agcagtaatg
    18961 tggttttggt taactagaaa acctaaaaaa aggaacaaga aaaatggttt ggaaaataaa
    19021 cgattattta gattaatgac aaatcattaa agctataggc atattaagag ataatattta
    19081 ccaatttcct gattgttttg aaaacgagca tctgtatcaa atcaaaaaca tgatgaattg
    19141 atttggatat tttgaaatag atattaagaa aaagcattcc ttattaaaac acaaggaatg
    19201 ctttttgttt tcacttaatc tgtttaatat aatctaacaa cttttcttcg gaattaattt
    19261 tttcacctag gtattgttca gacttattaa caagattcaa tttaagatca tttattattt
    19321 ctggatacgt atcacttttc gattcagcta attcatacag tttttttagt tccttatctt
    19381 ttaactgaaa cttctgatcc atagtgacag catttggata tagaagtcca ttataatgtg
    19441 tgcgtaaatc catgtggaat ttttgtttat aaaagtctag cagattagaa ataaaagaag
    19501 aaaaataacc gttatagtaa aactgattat ttctatttaa gttatacaac aatttattaa
    19561 attgatcctg atttattttg tcgtaaatcc atggcaatat ttccatgtcc ttatctgaga
    19621 atctaaatgc aggagaatcg gtcagatgct caatcataaa atctaagaac atctcttttt
    19681 caccaaaata tagatattga ttatatatcg tttctaaggt agaagttgct agagaagaga
    19741 ctctcatgtt ttgctgaagt attggatcaa gtagtctatt taaatgttct tgtaattctt
    19801 ccttggtgtt tcctagaact gcaagtgaac gtagacggta gtaatcttct atatgtgttg
    19861 ttattggctg ttttggttca aaatccttta ttgatatttt taagttttga attatttctt
    19921 ctgaaaaatc acgctgtaat tctggatgac tagctataaa gctaagcaaa aagaagcctt
    19981 ttgtataagt aatctgttga ttttctccaa gtgatttatt aatttcctct ctcgattcag
    20041 ctgtgaacct gtagtttgag atttcttttt ttagaatttt taaataaaat tctttgttat
    20101 caaaaatcag ggcatctagt agtctgaaag tacttactct atctctatct acttcttcat
    20161 tcccataagg taatttaaaa acaaacttaa aggctgtttt acaaaggttt tctttggctt
    20221 tctcacttag ttttttcaga tagctatttt ttagtgcaga gtggaattct tcaggtgtaa
    20281 cagacataga atgtattcga cttgcatatt gtatgatttc gttcgttact ttttttccta
    20341 atattggatt ttttagaaat acaatttcat aggatttccg tatcacatcc tgacaagttt
    20401 cctttgttat ttctcttaat tcccaagtgt caccattgta agtgactact ggatgagctg
    20461 aataatttct ttgatcctta atatatttta aatcctcata ttcgtagcta tcaattagct
    20521 cagtcgaatt ataagttttt tctagtagct ccatttccca tcttggggag tttggatcac
    20581 tttttcttaa tttagctatt tcttctaaaa ttttcttaga agtatcatcc tcgtatatat
    20641 ttgctaaatc agataattta tcaattaagt ctaaaacaag cgcactgtaa ttgacagcta
    20701 tcgctgaacg ataatttccc tgataaaaac tacttctgac ttcttctaat aaaggaacaa
    20761 ttctaggatt tttaactttg tttatttcat catctaatat aaacatataa ctctcctttt
    20821 tgttgatatg acaagtttac cacaa
//
LOCUS       DAZKKW010000047        20284 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.047, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000047 DAZKKW010000000
VERSION     DAZKKW010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20284
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.047"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(222..707)
                     /locus_tag="ACG8PO_001659"
     CDS             complement(222..707)
                     /locus_tag="ACG8PO_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HFQ0206971.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     misc_binding    797..1142
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1187..1828
                     /locus_tag="ACG8PO_001660"
     CDS             1187..1828
                     /locus_tag="ACG8PO_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFQ0206972.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIIMKQVEGKLNYYR"
     gene            1847..2482
                     /locus_tag="ACG8PO_001661"
     CDS             1847..2482
                     /locus_tag="ACG8PO_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFQ0206973.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            2500..3315
                     /locus_tag="ACG8PO_001662"
     CDS             2500..3315
                     /locus_tag="ACG8PO_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFQ0206974.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            3634..5070
                     /locus_tag="ACG8PO_001663"
     CDS             3634..5070
                     /locus_tag="ACG8PO_001663"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFQ0206975.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            5259..7706
                     /locus_tag="ACG8PO_001664"
     CDS             5259..7706
                     /locus_tag="ACG8PO_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFQ0206976.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            7936..9003
                     /gene="rlmN"
                     /locus_tag="ACG8PO_001665"
     CDS             7936..9003
                     /gene="rlmN"
                     /locus_tag="ACG8PO_001665"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HFQ0206977.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            8993..9199
                     /locus_tag="ACG8PO_001666"
     CDS             8993..9199
                     /locus_tag="ACG8PO_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0206978.1"
                     /translation="MPNNRILLVFSITIKGAVFLIKELFNLNKRKDSIAKAIKLFLTR
                     KTLIKQRNVLNLMTACLMGVLAII"
     gene            complement(9196..10299)
                     /locus_tag="ACG8PO_001667"
     CDS             complement(9196..10299)
                     /locus_tag="ACG8PO_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFQ0206979.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            10852..11400
                     /gene="aac(6')"
                     /locus_tag="ACG8PO_001668"
     CDS             10852..11400
                     /gene="aac(6')"
                     /locus_tag="ACG8PO_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HFQ0206980.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
     gene            complement(11647..11766)
                     /locus_tag="ACG8PO_001669"
     CDS             complement(11647..11766)
                     /locus_tag="ACG8PO_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFQ0206981.1"
                     /translation="MVFQKPLNNFRYIVYYCHTKIKKNKQKETVTKVLSQSLI"
     gene            11945..13606
                     /locus_tag="ACG8PO_001670"
     CDS             11945..13606
                     /locus_tag="ACG8PO_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HFQ0206982.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(13676..14023)
                     /locus_tag="ACG8PO_001671"
     CDS             complement(13676..14023)
                     /locus_tag="ACG8PO_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HFQ0206983.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            14107..15048
                     /gene="opp3b"
                     /locus_tag="ACG8PO_001672"
     CDS             14107..15048
                     /gene="opp3b"
                     /locus_tag="ACG8PO_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease"
                     /protein_id="HFQ0206984.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            15049..16092
                     /locus_tag="ACG8PO_001673"
     CDS             15049..16092
                     /locus_tag="ACG8PO_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0206985.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            16098..17168
                     /locus_tag="ACG8PO_001674"
     CDS             16098..17168
                     /locus_tag="ACG8PO_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0206986.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            17158..18111
                     /locus_tag="ACG8PO_001675"
     CDS             17158..18111
                     /locus_tag="ACG8PO_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0206987.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            18123..19133
                     /locus_tag="ACG8PO_001676"
     CDS             18123..19133
                     /locus_tag="ACG8PO_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HFQ0206988.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(19418..20239)
                     /locus_tag="ACG8PO_001677"
     CDS             complement(19418..20239)
                     /locus_tag="ACG8PO_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFQ0206989.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
BASE COUNT         6674 a         3558 c         4262 g         5790 t
ORIGIN      
        1 ttccgattta taatcgaaaa aagaagcatt tttatatatg cttctttttt cgtagaatca
       61 ataaatatgg atttggctaa ctatggaaga atactcgtat tagttttcag cgttcatact
      121 agttgttaca ttgtttgatt ggccaaaaga atttctgata tattttgtgc taaataaaga
      181 aaaagacgct tgaattggga aataagaaaa ctactctatc attacaaatt ttcataaact
      241 ggcagatgtt ctaagtaaac aagtatcttt tcaaagtcgg aaaaatccat aatcaacgta
      301 gtggtattga tatttggatg aaagcctacg gtatcttgac gatcaatagc agcatcgatc
      361 acgacttgga tttttttctc tttgtcgtgc atcaatgcta atggagtcac tgtacctgct
      421 ggaacgccta ggaggtcagg aagctgttct tcagaaacaa aagataagcg cttttctttt
      481 aattgttcag ctagttgttt taaatcggcg tgcttttcat caggcaagat gacaagatag
      541 atttgttttc cttttcttga tttcaataat agatttttta cttgcggacc aggtagggaa
      601 aaagggacat ttttgactga cgtaatcgca ggatgatcga ctttttgaaa agagataccc
      661 agttttttta gtagttcata agcttcttgt tcggttgcac gactcatcgc gcatcaccct
      721 ccttttttta ttagtgtatc ataaaaaaga gttgacaaat gaaaaataat catgtaaatt
      781 acacgtaaag ttaaaacaaa tgacatcgaa aagaagagta gccgttaggt gtttttagag
      841 agtctctggt tggtggaaag aggcagcagt taaccgtgaa acacatcttg gagtcgattt
      901 tctgaagcgt atagtaggaa aatccgggga gcccgttaca gctgcggtcg ttgctagatg
      961 accagataag gctggctttt ccgtgaggag tcggtaaaaa aaggtggaac cacgtgatta
     1021 acgtcctttg atcttcggat caaaggactt tttgttttca tctgaacggc aacgactgtg
     1081 tgaggttttt tctgcaagga aaaaataaac agaggtggaa ccacgtataa tacgtcctct
     1141 tggcatttgc caagaggatt tttttatttt aaaggaggaa aagaagatgg agtatttgat
     1201 tgaagttatg ccgcaattat tagtaggagc actcacaaca ttgaaattat ttttatttac
     1261 cttgattggc tcattaccat tagggattgt tttagcatta ggattgtctt tgcattttaa
     1321 gccgctacgc tacttgcttc aaacatatgt atgggttatg cggggaacac cgctgttgtt
     1381 gcaactgatt tttatttttt atgggttgcc gacgattggg atcgtttttg atcgtttcga
     1441 agcagcactc attgccttta tcttaaacta tgcagcttat tttgcagaga tctttcgtgg
     1501 agggatacaa tcgattcctc aaggtcaata tgaagcagcg caagttttgc aattaagtcc
     1561 ctttcaaacg gtgacgaaaa tcgtcatccc acaagtgaca aagattgttt taccttctgt
     1621 agggaatgaa gttatcaact tggtcaaaga ttcttccttg atctatatat taggtttagg
     1681 tgacgtaatg cgtgctggga aaatcgcgat ggaacgcgac gcgactttac tgcctttgct
     1741 aggcgtggca atcatctatc ttacattgac tggtattcta acaatcataa tgaaacaagt
     1801 tgaaggaaaa ttaaattatt accgctaact gataggagga agaaatatga ttgaattgaa
     1861 gaaagtgaac aaagcatttg gtgaaaaaaa aataatcaaa gatttgaatc taatcattcc
     1921 agatgggaaa acattagcta tcgtaggtcc ttccggtggc ggtaaaacca cgcttttacg
     1981 tatcttggct ggcttagaaa gttctgacag cggagaattt tatttagacg gtgtaccgtt
     2041 caacccattc gagcttaaaa tgaaagaaca agttgtagga gtcgtattcc aagacttcca
     2101 gctatttcca catctgaata tttttgaaaa cattacctta gcaccaaaaa tggtattgaa
     2161 acagacaaaa gaagaataca ccaaaaaagc agaagaatta gcggcttcat tagggattag
     2221 tgatttattg aatatctatc cttatcagct ttctggcgga caaaaacagc gggtagcgat
     2281 tgccagagca ttggctatga atccacgggt attggcttat gatgaaccaa caagtgcttt
     2341 agatcctgaa ttacgccagc aagtcgagcg tttgatccta tcattgaaag aagatggggt
     2401 aacccaaatt attgtcaccc atgatttagt atttgcagaa aatgtcggtg atcaattact
     2461 aaaagtttcc ccaattcatt aagaaaggag aagatagaga tgaaaaaaac cagttttcta
     2521 ttcgctttga tcgcaggtct tcttcttttt gcaggctgtt cgaacaaaaa aacttcagca
     2581 gataattggg cgactatcaa cgaagagaaa aaaatcgtta taggtttaga cgatacattt
     2641 gttcctatgg gatttagaga taaagacggg aagctcacag gatttgatat tgatttagcc
     2701 aaagcagtct ttgaacaata tgggattacg gtagactttc agcccattga ctggtcaatg
     2761 aaggaatttg aactgaacaa tggaacgatc gatttgatat ggaacggcta ttccaaaacg
     2821 ccggcaagag aaaagaaagt gcaatttaca aaaccttata tggaaaacga tcaagtgctg
     2881 atcacaccta aagaatcagg aatcacaaac tttggacaaa tgaataataa gagattaggt
     2941 gcgcaaaatg ggtcatctgg ttatgatgta tttacgaaac aaccagaagt attaaaagag
     3001 atagtcaaaa accaagatgc tgttttgtat gatagtttta atgaagcatt gatcgatctg
     3061 aaaagcagac gggtcgatgg tctgctgatg gataaagtat acgcagacta ttatttgaaa
     3121 caaagaaacg aactagaaga cttcaacata acaaaaggtt cttatgagag tgaggatttt
     3181 gcagttggtg cacgcaagtc agatagtaca ttagtgaaaa aaatcaatga agccctagat
     3241 caactagaga aaacaggtga attccaaaaa atatccgata aatggtttgg cgaagatgtt
     3301 acgccgagaa attaacaaat aggggactgt gacaaaagac gtgtcacagt ccctttttcc
     3361 gaataaacag tgttctcctg ccaactcctt gtgactcgga gctgaatggc aggctcacaa
     3421 cctcttggta aacggtgttc aaaagccagt ttttcggtat taagccaaat ttatgcaaaa
     3481 atgtagaaag ccattttcac aaaatttttc ttactactcg aaactaagcg actttttcac
     3541 aacctccttt acataatttt attttgttgt ggaaggggtt tcgtgttatt ctaaaagagg
     3601 aacgattgaa ggttatagga aaggaagaga tgaatggaac acaaagaact tacctcattt
     3661 ccaaaagatt ttctgtgggg ctctgcttct gcagcgtatc aagtagaagg agcttggcaa
     3721 gaagatggga aaggagaatc agtctgggat cgatttgtac ggatacctgg aaagacattt
     3781 aaaggaacga acggtgattt ggcagtagac cactatcatc gttataaaga agatattgct
     3841 ttaatgaaag aacaaggtct gaaagcttac cgcttctctg tagcttggac acggattttt
     3901 cctaatggac gaggagaagt caatcaagct gggctcgctt tttatgagag attgatcgac
     3961 gaattgattg aaaacgatat tgaacctatg ttgactttat atcattggga tcttccacaa
     4021 gcattgcaag atgaatataa tggttgggaa tcacgtcaga tcattgaaga tttcactcat
     4081 tatgcagaaa cattatttga ggcattccgc gggaaagtcc attactggat tagtttgaat
     4141 gaacaaaata tcttcacttc attaggttat ttgttagcag cacatccgcc aggtgtgaca
     4201 gacccgaaac gaatgtatga agttaaccat attgctaatt tagcaaatgc atctgtcatc
     4261 aataaatttc acgagttaga aatgcctgga aaaatcggac caagctttgc gtatacgcca
     4321 aattatccaa ttgacagtaa tcctgaaaat gttctggctg ctgaaaatgc agaagacctg
     4381 atggccaatt attggatgga tgtctatatg tggggaaaat atccgattgc tgccatgagg
     4441 ttcttagaag aaaaaggatg ggcaccaacg attgaagctg gcgatgcaga attgctagaa
     4501 tctgctaaac cagattttct tgggatcaat tactatcaaa cggcgacgaa tgcattcaat
     4561 ccattaaacg gagttggtgc tggaaaaatg aatacgacag gaaaaaaagg ttcatcagaa
     4621 gaaactggag ttcctggaat gtataaaaaa gttgagaatc catttgtcga acgaacaaat
     4681 tgggactggg agattgaccc agaaggatta aggatcggac tgcgtcgtat cacaagccgt
     4741 taccgcatgc cggtactgat tacggaaaat ggcttaggag agtatgacaa gttgacagaa
     4801 gataaacaaa tccatgacga ctatcgaatc aattacttac aaagccacgt caaagccatc
     4861 aaagaagcaa tttcggatgg agcagaagtc ctaggctatt gcacctggtc ttatacagat
     4921 ttgcttagct ggctgaacgg ttatcaaaag cgatatggat ttgtatatgt tgatcaagac
     4981 gaaacacaag aaggatcttt ggaacgatat aagaaagaca gttattactg gtaccaaaaa
     5041 ctgattgaag aaaatggaga gaacctttag ttcacatact taaaacaaga aggtacggct
     5101 ggatatggcc gtgccttttc ttgataaaat catttagaaa ttaagaaaaa ctgtaaaata
     5161 agctgaaaaa tgaaagaaag cgtcagttct ttcaaacaaa actttagttc ccttactttt
     5221 ttttgtataa tgaaacttga tgaaatgagg gataataaat ggcacaaaag aaacggccta
     5281 ctaaaaaaag gaaaaagacg aaaaaaacga aaaagcaaaa acagcagcag gaacaaatgc
     5341 tgacgattgg tgttgggttt gccttgattt tatttgctgt cttcggcttt ttaaaattag
     5401 gttttttagg aatattgatt gcaaacggct ttcggatcat tgcaggaaat acttaccaaa
     5461 tcctctgtct tttattagcc gtattaggat tttggattgt cattaagaat acagagtttt
     5521 caattggtaa aaaccgacgc tggtttggcg gcatactctt ttatttcggt atcttattat
     5581 tgttacatgc ccatttattt ggcaaactcc atactgggga accaaatatc atggggacga
     5641 catgggattt gttagccagt gatataaaac aaagtcaagt agacaataac gttggtggtg
     5701 ggatgattgg agcaatcctt tatcatttta cttacttctt gattgcacag cctggaagtt
     5761 atttagcagc agtgctatta ctggcaggag gagcattcct gttcagtaat ctggaaggct
     5821 atcagctgtt aaatggtatc caatcaattg gagaacgagt acaggaatta ttggaaggtg
     5881 atcctgccaa gcaagctcga aagcaagcgg caaaggaaga gagaatgaaa cagcgcgcag
     5941 aagcgaaaga agcaaggcgc ttagctgcaa aagaagctgc agaaaaagaa gctgcagaat
     6001 atgagaagaa aaaagcggtg agccagcaaa gggaaaatac tgctgatgaa tggcaggaac
     6061 cagaaaatac agagccggag cagctaagtt ttgttccaat cgacagtttt caagaaaata
     6121 tccatcctgc gaatctagag aaaccagtac cagatacacc aaaacagacc aatacagcag
     6181 aaggtttttc agatgagttg ccagaggatg acggtacctc attagaattt gagattgaag
     6241 cagaacagga aaatcaagac tatgaattac cgacagttga tttgcttgat tctataccaa
     6301 ctgtggatca aagtgatgag tataaaaaaa ttgaaaaaaa tatcggcgta ttagaacaaa
     6361 ctttccaaag tttcggtgtc gatgccaagg tcgtcaaagc cagcttagga ccatccgtca
     6421 caaaattcga agtccagccg gctgtagggg taaaagtaag taaaattgtg aacttaacag
     6481 atgatatcgc gcttgcttta gcggcgaaag atgtccggat ggaagcaccg attccaggta
     6541 aatcactgat tggtattgaa gtaccaaatg gaaagatcag tatggtttcc tttagagaaa
     6601 tcatcgaagc acaaccaaac cacccagata aattactaga agtaccttta ggacgtgatg
     6661 tttctggacg ggtacaaacg gctgatcttt cgaagatgcc tcacttactc gtggctggtt
     6721 cgactggtag tgggaagtca gtagcaatta atgggattat cacaagtata ttaatgcgtg
     6781 ctaagcccca tgaagtaaaa ctgatgatga tcgatccgaa aatggttgaa ttgaatatgt
     6841 acaacggcat acctcatctg ttgacgccgg tcgtgacgaa ccctagaaaa gcagcgcaag
     6901 ccttacaaaa agtagtacaa gaaatggaag aacgatatga aaaattcgcg gcaaccggag
     6961 taagaaatat ttctggttac aatgaatttg ttcaacagaa aaacttggaa aacggaacga
     7021 aacatccaac cttaccattc atcgtagtga tcgtagatga attagctgat ttgatgatgg
     7081 tagccagcaa tgaagttgaa gatgcgatta ttcgtttagc gcaaatggct cgggcagcgg
     7141 ggattcatat gatcctagct actcaacgtc caagtgtgga tgttatcaca ggaatcatca
     7201 aagcaaatgt accttccaga atggcatttg cggtatcaag cggtacagat tctcggacga
     7261 tcattgattc taatggggca gaaaagctct tagggcgagg agatatgcta ttcttgccaa
     7321 tgggtgagaa taaaccgatc cgtgtgcaag gagcattcat ttctgaccac gaagtagaac
     7381 gggtcgttca atttgttaca gatcagcaag aagctcatta tgaagagaaa atgatgccga
     7441 cagatgaagt tgaaacgaca ggtgctccag aacagccaca agatgagctg tttgaagaag
     7501 caaaagcact tgtcgttgaa atgcaaacgg caagtatttc ccttctccaa agaagatttc
     7561 ggatcggtta caaccgagca gcacgtttag tagatgaact ggaagctcat ggagttgttg
     7621 gcccgtcaga aggaagcaag cctcggaaag tattcattga gcaggaagaa acatcagaaa
     7681 cgttgccgga cgataatgac atgtaaaact tgtgtgatga agggttgaaa aacgaataac
     7741 caataaaaag acaaggcatg atgataccaa aagaaaaaat ggtttttttc catcttttct
     7801 aatacggtat gatgtcttgt ctttttagtt tttgtgagat taaagttcta ttcgatttat
     7861 ttgtgaagta ttgcattaag gaaatgaatc atgtatagta tgtagcgaaa ctttgagaaa
     7921 aagagggaaa atactttgga aaaacagtcc atttatggct tgacgaatga agaattaatc
     7981 aattggttta tagaaaacgg cgagaaaaaa ttccgagctg cccaagtttg ggaatggttg
     8041 tatcaaaaac gtgtaagtaa tttcacagaa atgacgaatc tatcgaaaca attgatcgaa
     8101 aaattatcgg agcattttat catcaatcct ctaaaacaga tggtcgtcca agaagcttcg
     8161 gatggtacag taaaatattt gtttgaactc cctgataaaa acatgattga gactgtactg
     8221 atgagacaag aatatggctt gtcagtttgt gtgacgacac aagtaggatg taatatcggc
     8281 tgtacgtttt gtgcaagcgg gctgctcaaa aagaaccgtg atctgactgc tggagaaatt
     8341 gttgcacaga tcatgatggt ccaacattat tttgatgagc gcaatcttgg cgaacgcgtt
     8401 tcacatgtcg ttgtaatggg gatcggtgag ccatttgata attacgacaa tgtaatggac
     8461 tttttgcata tcatcaatga tgccaaagga ttggcaattg gtgctcggca cattacagtt
     8521 tccactagtg gattagcaca caaaatcaag gaattcgcta ataacggctt gcaagtcaat
     8581 ctggctattt ccttgcacgc gccgaataat gaggtgcgaa catcgatcat gcggatcaac
     8641 cgaagtttcc caattgagaa attgatggaa gctgtggatg agtatttaga gaaaacaaac
     8701 cgtcgaatca cgtttgaata tatcatgttg aatcaagtaa atgatagacc agagcatgcg
     8761 caacaattag cagatctatt aaaagataag aaaaaattag catatgtcaa cttgattcca
     8821 tataatccag tcagtgaaca tgatcaatat tctcgcagtc caaaagctga tgtattgaga
     8881 ttttatgatg tattgaagaa aaacggcgta aactgtgtga tacgtaaaga acatggtaca
     8941 gatattgatg cagcttgcgg acaattgcga agcaagcaaa tgaaaaaaac agttgccaaa
     9001 taatcgtatc ctactcgttt tttcaattac cataaaagga gcagttttct taattaagga
     9061 actgttcaac ttaaataaga gaaaagattc tatagcgaaa gctataaagc tttttttgac
     9121 aagaaaaacc ctcatcaagc aaagaaatgt cctcaatttg atgacagcct gcttgatggg
     9181 ggttttagca attatttaac tgattttctt atatccttgc tggctttctt gtgtagcatg
     9241 ataagtttct acgtcaagaa tggcctgttt aaatttttct cgggtgattt cataaggcac
     9301 atgtttaata tcatttgttg ccatggcttt atctaagatt gcttctaaat cggtcgtgac
     9361 attgagattc atgtcggtta acgatactgg aagttggttc tttttcataa acacatagat
     9421 tttatcaaac aaatcgaact gctgatccat catcaataag gtcagtacac cataactgac
     9481 agatgctccg tgaaggtatc cctccatttt ttctagaaca gtcgtaccgt aatagaatga
     9541 gtgtgccata gcagaattat agttattgtt aactaaaaca gaaaccaatc cggtagtaac
     9601 aatgatatcc gaaacgactt gttcaattgc atatgaggga cgatcgtcgg cactatcttt
     9661 caatgcttgc gtaccataag tcaagagacg atcaatgcac atttttccga tttggattcc
     9721 taatctatcc gtgtagccta gatcatcgcc acgagcagaa aaagtagatt cgacttcttt
     9781 ggataaagca tcgccaatac ctgcccataa gtatttatca ggtgcttcgg caataattgc
     9841 tgtatcaatg aaggtgtgta caggtggttt tgaaggaaag aagagtccgt ccattacgcc
     9901 ttcttcgtta tagatgacac ataccgcagt gacgggcgaa cagttcgaac caatagtggg
     9961 aaaggtgaag actggtttag ctagtttttc accaatgact tttacagtat cgcaagcacg
    10021 gccaccaccg actgcaaaca ggacgtctgc tttttggaca ctttcatgct ggatcaactg
    10081 ttcaatattt ccataagttg aattaccccc gtaccagaga ctggcgacaa cctctatttc
    10141 ctgtgtcaaa gctactttta ttttgttttc tgcctttgaa agtgcagtct tccctccaat
    10201 cagcactact tttgtcccat attctttgac gattccattt gctttttcat acgcatctgt
    10261 tccaatggta taatttggta agaaaacact ttgactcata ttcatttcct tctttcttcg
    10321 ttgaaacggg ttaaaatttt aaagagtgat cgatctgact gcggctgtag atggagtaga
    10381 ggtgaaccta agacaggata agtgatgaat aaggtggcat gaacaccttg aaatcatcaa
    10441 taagtatcct aaaaacgaac aagtacaaaa acattgaaaa cgacaataat gaatttttgt
    10501 tcctcttagt atttctaccg attccctttg cttttttgta attatctaaa aattctaaaa
    10561 attaagatta ttatatgaaa acaggtttta tttgtcaatt cataattggt tttttttaaa
    10621 gcaaaagata atctatatca tgatgaacaa acagagtttt ttcctccaat tgatggcttt
    10681 cctgatcttt cctattttga ataaagtgtt tctgtataat ttttttattt tataagtaag
    10741 ataaataatg aaagaataga gatactgatt ggtagctaaa gatttttgag cgagaacatt
    10801 tcgttttttc aaaaggactg ttagaataag caagaacaag ggggaagacg tatgataatc
    10861 agtgaatttg accgtaataa tccagtattg aaagatcagc tttctgattt actgagactg
    10921 acttggccgg aagaatatgg agacagctcg gcagaagaag tagaagaaat gatgaatcca
    10981 gaacgaatcg cggtagcagc ggtagaccaa gatgagttag taggatttat tggtgcaatc
    11041 cctcaatacg gtatcacagg ttgggaattg catccattag ttgtagaaag ctcccgacga
    11101 aagaaccaaa taggtactcg attagtcaat tacttagaaa aagaagtagc ttccagagga
    11161 ggaatcacga tttatttagg tacggatgat ttagaccatg gaacaacgtt aagtcaaacg
    11221 gacctgtatg agcatacatt tgataaagtg gcttccatcc agaaccttcg tgaacatccg
    11281 tatgaattct atgaaaaatt aggttataaa atcgtaggtg tcttaccaaa tgcaaatggc
    11341 tgggacaaac cggatatttg gatggcaaaa acgattattc ctcgaccaaa ttctcaataa
    11401 taaaaagagg ttgtgacaga agtaatcagc ttcaagaaat aagaaggaat ttacgaaaat
    11461 tacttcttga atttttgtga aattccaact tatttccgaa gaagctactt ttggaacacc
    11521 gtgtaccaga ggttgtgaaa aagtcgctag tttcgagtag taagaaaaat tttgtgaaaa
    11581 tggctttcta catttttgca taaatttggc ttaataccga gaaactggct tttgaacacc
    11641 gtttattcag ataagggact gtgacaagac ttttgtcaca gtttcttttt gtttattctt
    11701 ttttatcttt gtatggcaat aatagacaat ataacgaaaa ttatttaatg gcttttggaa
    11761 gaccatggaa aaccttgtac aaagctattt tttctgaaaa attccttgaa acctcacgaa
    11821 aaaatcatta ttttatgaaa aagacaaaaa aaagtgttga ttttttaata attattcatt
    11881 aaactcttaa acaacttcag aaaatattaa ttattctgaa aattccaata aaggggtacg
    11941 taaaatgaaa aagaaattta catttggttt ggttgcggta tgcggccttg tacttgctgg
    12001 atgttatggc ggcagttcag ccacaagcag tagttcagca aaaagcggtt cagcagatgg
    12061 aggaggcgta tttaacttag tcgtgcctca agaaatgcct acagctgact tatcagttgc
    12121 aacagataca atcagtttta cagcattgaa taacgtttat gaaggaattt accgtcttga
    12181 tgaagacagc aaaccacagc cggcaggagc tagcgagttg gcagaagtca gtgaagacgg
    12241 attaacttac aaaatcaaat tacgagaaga cgcaaaatgg tcaaatggtg atcctgttac
    12301 agcagcggat tacgtttatg gctggcaacg aacagtagac ccagctacag cttctgaata
    12361 tgcttacctg tatgcgccag ttgaaaatgc agaagacatt acagctggaa agaaagataa
    12421 atcagaacta gggatcaaag cagttggtga ctatgaatta gaaatcaaat tgacgaaaca
    12481 aacaccatat ttccaatatc tgcttgcttt cccatcattt ttcccacaaa aacaatccgt
    12541 agtggaagaa aatggggatg cctatgcatc tgcaagtgat aaagctgttt ataatggacc
    12601 attcacatta gaagattttg atggaccagg aacagataca gaatggactt ataagaaaaa
    12661 tgatcattac tgggacaaag atacagtaaa actttctgaa atcaaagtca gtgtagtaaa
    12721 agaatcttct actgcattga acttattcaa agatggccaa gcagatgacg tgatcttgtc
    12781 tggtgagtta gcacagcaaa acgcaaacga tccagcttat acttctgtta aagaagcgcg
    12841 gacaagctat atcgaattca atcaacgtga aaagaattca ccattcaaca atgtgaacct
    12901 aagaaaagct atttcttatt ctatcgatcg tgaagcttta gtaaaacaag tactaggtga
    12961 cggatcagtt gtctctacag gattgatccc atcagatatg gtaaaaagtc caacaacgaa
    13021 cgaagacttt gcaaaagaag ctggaaaatt agtttcttat gacaaagata aagcaaaaga
    13081 atattgggaa aaagcgaaaa aagaattggg cgttgattct ttagaatttg atttgatggc
    13141 ttctgatgat gattcatcga aaaaagtaat cgaatatgtt caaaactcaa tccaagaaaa
    13201 cttggatggc gtaacagtca aaccaacacc tgttccattc tctgtacgtc ttgaccgctc
    13261 aacttcgggt gattttgacg cagtactagg cggatgggct gctgactatg cagacccaag
    13321 cagctttact gatttgtttg tcacaggtaa ttcatacaac cgtggtcgct ggagcaatgc
    13381 agaatatgac gaagcagtta aagcagcttc atcaacagac gctggcgatg aacaagcccg
    13441 ttgggatgat ttccaaaaag caatcaagat catctctgat gatatgggtg tcgttccggt
    13501 gtaccaaaaa gcagaaggtc acctagtgaa tgaaaaaatc aaaggtatcg ttcaccatgc
    13561 ggcaggcgct tcatgggatt acaaatggac atacgttgaa gaataatcgt taataccgtg
    13621 agcatgagaa aaaacgaggc tgcggcaaat gtcgcaacct cgttctcata ttcacttatc
    13681 ctaacaaaga aaaaagcatg aaaatagcag aaagagcaat tagtattccg gcaatgatca
    13741 gccaaaagct gtacataccg tagctggcag acgagagcga ggaaaattct ggttttttct
    13801 ttcggttcct tgttcgtgca agatggaccg atcgctgaaa atgatcgaag aaacccgaag
    13861 aaaagaccca taaaaaaccg ccgataatca gaaacggcaa tgaccataag aatagatcat
    13921 tagataattg aatgagtgta aaggaatggt tgatgagatt ttttattaaa ataaccagaa
    13981 tcagtagaaa agctactgcg taggggatag attttttttt catttgattg ctcctttatt
    14041 tttggttcat tatctaataa tgtagcgaaa catctgtcag aagtcaaaag ctagggggaa
    14101 acattagtga atagttatat caaatatgta ttaaaacgtg tctttttcat gatcatcacg
    14161 ctttggttaa ttgcaacaat cacctttttc ttgatgcaat tgctgccggg cacgccatat
    14221 acgaatcagg aacgtttgag tcctgaaaca atcgagatgc taaataaaca agtcggtttg
    14281 gataaaccag ttatcgtaca atatgggatt tatctttcta atttgcttca aggagatttt
    14341 ggaatttcct tccaatttaa aaaccagcca gtcgcacatt tgttagctgg aagaatcgga
    14401 ccatccttgc agttagggtt acaggcaatt atctttggaa cagtcctagg gacgatttta
    14461 ggaacgatct ctgccatgaa gcaaaataca tgggcagata catcttctac actcgtagcg
    14521 atattaggtc gttccattcc aaactttgta tttgctgttt tattacaata tatctttgca
    14581 atcaaattac aagttttgcc aatcgcaaaa tgggatggct ttgtgtacac aatcttacca
    14641 acaattgcgt tggccatgtc gccgcttgct gactctgctc ggtttatccg tacggaaatg
    14701 gtggaagtac tgcatagtga ttatgtggaa ttagcaaaag caaaaggatt gagccgttgg
    14761 gaaatcgcct ttaaacatgg tcttcgaaat agtttgattc cattgatgac tttattagga
    14821 ccgttagctg tagcattgat gaccggatca ctggtggtag aaaatatctt tgctattcct
    14881 ggtatcgggg aacaattcgt taagtcaatc acaacaaatg attatcctac gatcatggct
    14941 gtaacgatcc tttactcatt tatgctgatt tttgttattt tgatcgtgga tctattgtac
    15001 ggtctagttg atccaagaat tcgtgtttca gaagggagcc gaggataaat ggaaatgata
    15061 ccaaagcgtt atgaaacagt tgcagatatc cctgcggatg aatttcttcc attacaaaaa
    15121 aataccgaag aagaacgtga acagattact gcaccttctt tgaattttat ccaagattca
    15181 tggcgccgtt taaagaaaaa caaagctgcc gttatttcaa tggttttact attggtcatc
    15241 attttgatct ctgtgatcac aatctttgtt tctcctcatg atccaactgc ccaaaacgtg
    15301 gcttatatca atttgcctcc tcgtattccg ggaatcaata tcgatggatt gaatggaaaa
    15361 gcaatggttg gtggtgaact tgttgataaa tatgctcaag caaacgtccc ttcaaacgta
    15421 aatttctttt taggaacaga cggtttggga cgtgatgtat taagccgctt atttatggga
    15481 atgagaatct cgttgctgat cgcatttatt gctgcattac tagatgtaac gattggtgta
    15541 gcttacgggt tggtttctgg tttgttaggc ggacgtgtcg ataacgtgat gcaacgtttt
    15601 ttggaaatct tatcaggaat tccaaacctc gttgtcatga ttttgatgtt ggtcgttttt
    15661 gaaccaggaa ttttttcaat cgtggctgct atggccatca caaactggat accaatggca
    15721 cgtatcgttc gtgcacaaac aatgaaatta aaagatcaag aatatgtttt agctggattg
    15781 acattaggtg aatcaagaat gaaaattgca ttcaaacata ttttgccgaa tatctctagt
    15841 gtcattatta ttcaaatgat gtttagtatt ccatctgcta ttttcttcga agccttttta
    15901 agctttatcg gtttaggtct tacgcctccg tcagcttcat taggaacgat gttgagcgaa
    15961 gggtacaaga cattcctgta tctgccttat ctattatgga ttccggcagt aactttatcg
    16021 gttatcatga ttggctttaa cttacttgct gatggattgc gtgatgcctt tgatcctaaa
    16081 atgaaagagt gaagacgatg acgaaaattt tagaagtgaa agacttagaa atctcttttg
    16141 atacgtatgc tggaaaggta cgagcgattc gcggtgtcaa ttttcatttg aataaaggag
    16201 aaacattggc gatcgttggg gaatctggaa gtggaaaatc agtgacgacc agaagcatca
    16261 tgcgcttgct gtcaagcaac gccaatatag atgctggtca aatcctcttt aaaggtaacg
    16321 atatcgttgg aaaatcagaa agagagatgc aaaaaatccg tggtaaagaa atagcgatga
    16381 ttttccaaga tccaatgacc tctttagatc cgacgatgcc cattgggaag caagtcgctg
    16441 agtcccttag aaagcataat aaagtttcaa aaaaagaggg acaaaaagct gcattagaat
    16501 tgcttaaatt agttggaata cccgaagctg agaaacgtat caacagttat ccgcatcagt
    16561 tctcaggagg tcagcgtcaa cggatcgtga tcgcgattgc actggtatgt tatccggaaa
    16621 tcctgattgc tgatgaacct acaacagcgc ttgatgtgac tattcaagca cagattttgg
    16681 agctgctgaa agaaattcag acaaaaatcg atacatcaat tatctttatc actcatgacc
    16741 ttggtgtagt agcgaatgtc gctgatcgtg tcgctgtcat gtatggagga agaatcgttg
    16801 aagttggaac atcggaagaa attttctaca acccgcaaca tccctatact tggggactgc
    16861 ttggttcaat gccgacatta gatagtgcga acgatcgatt gtatgctatt cctggttctc
    16921 caccggattt attgaacccg ccaaaaggag acgcattcta tccaagaaat gaattcgcga
    16981 tgaaaatcga tgcagaacaa gaaccaccat tttttgaact atcaaaaaca cacaaggcag
    17041 caacttggct acttgctccg caggcaccca aagtgacacc gccagaagaa atccaacgtc
    17101 gctgggctat ctttaaagag aaacaaaaac atacttacgg tcaaggagga attgctgatg
    17161 ccaaataaac tacttgaagt taaaggattg aaacagtatt tcaatgtagg aaaaaagaat
    17221 gaagtacatg ctgtaaatga catcagcttt cacatttatg aaggagaaac attcggttta
    17281 gttggagaat ctggaagcgg aaaatccact actggccgta cgatcattcg cttgaatgaa
    17341 cctactgatg gtgagatatt attcgacggt caagatataa cgaagatcaa agataaaaaa
    17401 ggtttgacga aattccggca tgatgttcag atgatctttc aagatccata cgcttcgttg
    17461 aatcctcgta tgaaagtcag agatatcatt gccgaaggaa ttgatgttaa tggactagct
    17521 aaatcatctg aagagcgtgc aaaaaaagtc gatgatctat tgagaactgt tggattgaat
    17581 ccaagccacg gtactcgtta tcctcatgaa ttttcaggtg ggcaacgcca gcgtattggt
    17641 attgcacgag cattggcagt aaaaccgcgt tttatcattt gtgatgaacc aatttctgca
    17701 ctagatgtgt cgatccaagc ccaagtggtc aatttgttgc aagatctcca aaaagaacat
    17761 caattgacct atctatttat tgcacatgat ttgtcgatgg ttaaacatat tagtgaccga
    17821 atcggtgtaa tgcataacgg cttgttatta gagatgggaa caagtgatga aatctataat
    17881 cacggtgttc atccttatac agaaagtttg ttatctgcta ttcctctacc agatccagac
    17941 catgagagaa aacgcagaag aatcaaatat caacctgaac cagatgacgg acaagtacgt
    18001 caacttcgtg aaatcgcacc agaacatttt gtctatgcaa ctgagcagga agtcccatat
    18061 tacgcaaaaa aactaaaacg acaaaaggaa tctcttctgg ttgctaacta ggagggacat
    18121 agatgctaaa agcttttaaa ttccgtatgt atccgacaga agaacaaaaa cagcaattga
    18181 ttcgtacttt tggatgtgct cgatttacct ataaccattt gctgaaaaag cggcagaaaa
    18241 gttggcaaca aacaggagta gccaattttt cgctcacacc cgctacatta aaaaaagaat
    18301 acccattctt aaaagaagta gacagtcttg ctttagcaaa tgcacagctt aatttggatc
    18361 gggcttttcg caattatttc aagggacgtg ccagcttccc aaagcttaaa acgaaaaaaa
    18421 gtatgtggca atcttatacg accaacaacc aaacccgtac gatctatctg gaaaatggct
    18481 acttaaagtt accaaaacaa aaagaactaa tcaagataaa tcagcatcgg ccagttgaag
    18541 gatcgattcg ctccgctacg atttctgctc gatataatga agaattttat gtcgcactac
    18601 tttgtgatgt ttcgccagta aaaaaagaat ctttagctaa atggatcggg atcgcctatc
    18661 atccgaagac attgatccaa acatctcgac ctctagaagt aactttacca aaatttcacc
    18721 agacagaaga aaagctgcaa catgcacagc ggaaattgaa tgtgaaagtc cgctccgctc
    18781 accatagaaa gatcaggtta gatcaggcaa gcaattatca gaaacaaaag agaaaagtaa
    18841 tggatcttta tctgaaacaa aagaaccagc gagaagatta tcttgaacaa ttatctggga
    18901 aattggtcaa acaatatgac tacttatttg tagaatcttt tccaaaagaa gaggcacatg
    18961 ctgatttttc tattcacgac tggcataaat taataacaaa attgcgttat aagtcccaat
    19021 ggtataataa aaaatttctt ttgatcaata ccgacggagc agaagaaagc aattccgtaa
    19081 gaaagagcca agtagtagaa aaaatgggca gacattcggt tatcaaggga taaaaaatga
    19141 atgaaactga taggagcgtt agggttagcc ttgttaataa aaagtcttaa tgacttttgt
    19201 ttctaggaat ttttcttttg aagaaaagct cactaattat gacaaaccaa taaaacaata
    19261 gcagaaaaag ttcgtacgag ctttttctgc tattgttaat tttttattca gaaagtttac
    19321 gaatacatca aaattctgaa acaaaaaacg taactcacaa agagttgttt ttgcgttttt
    19381 tcttgaatga ttaggtctga atcttttgct cacaacctta ttcatggtgc cccggcttga
    19441 gctgatagat ttttgaagga cggccgattc ctacaggacg ttcaccgatc tcatcgaaat
    19501 attggagcat agctttctta aaattggaat gatcgatcgt tttataatcg atccctaaga
    19561 atttagcaaa tacttttcga gcttccgtaa tcgtgaaatc tttacctaaa acttgtagaa
    19621 cttgcggctc gtgttccatt ttattgacta cacggttgaa tgctttcaag atgatttgac
    19681 tatggtcaaa agccaaagta tctttgccag aagactctcc agtgactaaa tcaagcgaga
    19741 tagccacttc tccgctagat aaatggatct tgttactatg acgttctagt gtgaaccagc
    19801 gtacttcctt ggcatcatct ccggcgatta gcggttcttc tccaataaag gctaagtagc
    19861 tgactgtgac gacccagcct cgcggatctc gattaggcgt actgaatgta tgcagttgct
    19921 caatattttc ttttgaaata accacaccag tttcttcctt tgtttctctt aagacgcttt
    19981 ctccagttga ctcatccttt tggacaaagc caccaggcaa tgcccatgaa tttcgataag
    20041 gatgcccttt tctttggatc aacaaaactt tcagctgatc ttcttctttg ttatagcaca
    20101 tcagtacgat atcgacagtc aaagagggct tgtcatattc tggacgttcc tgtttttgat
    20161 accagttaag aaattcttct tcacttgctt tatgttcata atataatttt tcttcttctt
    20221 tagaagcaaa acggttcatc tgaccacctc cattgacagt aaaagtactt tttaccttat
    20281 tgta
//
LOCUS       DAZKKW010000048        19668 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.048, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000048 DAZKKW010000000
VERSION     DAZKKW010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19668
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.048"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(84..1031)
                     /locus_tag="ACG8PO_001678"
     CDS             complement(84..1031)
                     /locus_tag="ACG8PO_001678"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFQ0206990.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            1277..1741
                     /locus_tag="ACG8PO_001679"
     CDS             1277..1741
                     /locus_tag="ACG8PO_001679"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFQ0206991.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQQILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            complement(1803..2846)
                     /locus_tag="ACG8PO_001680"
     CDS             complement(1803..2846)
                     /locus_tag="ACG8PO_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HFQ0206992.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGIKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLFYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(3346..4311)
                     /locus_tag="ACG8PO_001681"
     CDS             complement(3346..4311)
                     /locus_tag="ACG8PO_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HFQ0206993.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            4545..5390
                     /locus_tag="ACG8PO_001682"
     CDS             4545..5390
                     /locus_tag="ACG8PO_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFQ0206994.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEIANSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            5581..6768
                     /locus_tag="ACG8PO_001683"
     CDS             5581..6768
                     /locus_tag="ACG8PO_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFQ0206995.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            6774..7130
                     /locus_tag="ACG8PO_001684"
     CDS             6774..7130
                     /locus_tag="ACG8PO_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HFQ0206996.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            7132..7470
                     /locus_tag="ACG8PO_001685"
     CDS             7132..7470
                     /locus_tag="ACG8PO_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HFQ0206997.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     misc_binding    7546..7802
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            7865..8899
                     /locus_tag="ACG8PO_001686"
     CDS             7865..8899
                     /locus_tag="ACG8PO_001686"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFQ0206998.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            8892..9578
                     /locus_tag="ACG8PO_001687"
     CDS             8892..9578
                     /locus_tag="ACG8PO_001687"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFQ0206999.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            9602..10450
                     /locus_tag="ACG8PO_001688"
     CDS             9602..10450
                     /locus_tag="ACG8PO_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFQ0207000.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            10647..11420
                     /gene="sufC"
                     /locus_tag="ACG8PO_001689"
     CDS             10647..11420
                     /gene="sufC"
                     /locus_tag="ACG8PO_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HFQ0207001.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            11433..12719
                     /gene="sufD"
                     /locus_tag="ACG8PO_001690"
     CDS             11433..12719
                     /gene="sufD"
                     /locus_tag="ACG8PO_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HFQ0207002.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            12716..13951
                     /locus_tag="ACG8PO_001691"
     CDS             12716..13951
                     /locus_tag="ACG8PO_001691"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HFQ0207003.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAH
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     SFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVINPVQELAELVHENGGVIVVDGAQSAPHMTIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQELGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VIAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFKHGTI"
     gene            13938..14408
                     /gene="sufU"
                     /locus_tag="ACG8PO_001692"
     CDS             13938..14408
                     /gene="sufU"
                     /locus_tag="ACG8PO_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly sulfur transfer protein
                     SufU"
                     /protein_id="HFQ0207004.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            14413..15804
                     /gene="sufB"
                     /locus_tag="ACG8PO_001693"
     CDS             14413..15804
                     /gene="sufB"
                     /locus_tag="ACG8PO_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HFQ0207005.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(15899..17041)
                     /locus_tag="ACG8PO_001694"
     CDS             complement(15899..17041)
                     /locus_tag="ACG8PO_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0207006.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(17080..17814)
                     /locus_tag="ACG8PO_001695"
     CDS             complement(17080..17814)
                     /locus_tag="ACG8PO_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0207007.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIIEEFDNNILLLTSSIEEQLEEMIDEGIHSQVEDMNYPEEAVNEIIDN
                     INKLKQTISSIGKKYTKKKMKGGDTI"
     gene            18049..18324
                     /locus_tag="ACG8PO_001696"
     CDS             18049..18324
                     /locus_tag="ACG8PO_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0207008.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMEQYKG"
     gene            18422..>19668
                     /locus_tag="ACG8PO_001697"
     CDS             18422..>19668
                     /locus_tag="ACG8PO_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0207009.1"
                     /translation="METSMINIPIYVDQEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSVEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYG"
BASE COUNT         6451 a         3562 c         4057 g         5598 t
ORIGIN      
        1 tttattcgga aaaaaggact gtgacaagac ttttgtcaca gtcctttttc atttaaaaaa
       61 atgtttactt catttgacgt atattattgg tgaggcttca attcatttgg cttcactttt
      121 tctgtttctc ttatgatata aacaggacgc tgtttcgttt ctaagaagat tttcccgata
      181 tatttcccga tgattccaag acataacaat tggattccac caatgaacag gaagatcgag
      241 accatggaag gccatcctga tgtaggatca ccaaacaaca atgcccgcaa aataatgaac
      301 aaaattgcaa taccagaacc gacacaagac aaagcgccga taaatgaagc aatcgttaac
      361 ggtgcatccg agaagttcac gataccatca atcgagtagt tgaataattt ccagaaggac
      421 caagatgttt cgccagccac acgttcgcgg ttttcataag aaatatattc tgttttaaac
      481 ccgacccagc taaataatcc ttttgaaaat cggttgtatt ctgttaattc taggacggca
      541 tccaccattt gacgagtcat caagcggaaa tcacgagcgc catcgaccat ttctgtatcg
      601 ctgattttat tgatcaactg gtagaatttt ttcgcaaaga aactgcgtat cgcaggctcg
      661 ccttcacgag tcgaacgccg ggtgccgaca caatcgatat cagaagtagt gatccggttg
      721 atcatctcag gaagcaattc gggcggatct tgcagatcaa catccatcac ggtcactaaa
      781 tctcctgtag aagcttgcaa tccagcgtat agagccgctt ctttcccaaa atttctggaa
      841 aatgagacaa agcggacata aggataagct gaggctagct tcttgattgc tgctaacgta
      901 ccgtcttttg atccgtcgtc aataaacaaa tattcgaact catgggcacc tttgaatttt
      961 tcaacctcat caaaaaaaag aggaacagtt ttttcttcat taaaacatgg gacaacaatt
     1021 gatactttca tcgtttctat cctttcaatc gtatgtttat atatggttta cattcaaaat
     1081 ttaataaacc ttaaataaca cctttaaaat ttgattttac gtaaaagaac aggtattctt
     1141 tttgtaaact aacgtattta gtttaccatg tttcctcggt ttatcaaact tttaagaaca
     1201 atttttatta gttggcaagt agttttggat agagaaaaag gaggaagttc cagtctattg
     1261 actagaagca aacaaaatga aaatacacac acctatcggc tttttatggt tagatgcaga
     1321 tgaatccgga ttgactgctg tatcttttca gccaatttca gaaagcgaat cagcgaatca
     1381 gcaaatttta acgcgcacac aagaacagtt tgaagaatat tttgcgggaa aaagaaaaga
     1441 atttgatgtt cctttatcta taaagagagg tacgcctttt cagcaaaatg tctggaaagc
     1501 tttgtcgacc attccttatg gagaaatccg aacgtataaa gaagtagccg ttgctgttga
     1561 tagtccgaaa gctgtgcgag cgattgggca ggcaaatcga accaatccat tgccaatcgt
     1621 tattccttgt catcgagtga tcgggcaaaa tggaaaactg acaggatata tgggaaacgc
     1681 aatagatggg gttgaaatca aacgccaatt gttgttgtta gaaggatatc tgcaaaatta
     1741 aaaacagttg tgattcggac attgttccat gcgagaatca caactgtttt ttcatgttca
     1801 ttctaaactt tgatcagcca aataaatagt aaagccatca ctgtttggag aagcccgaca
     1861 gctaatccat aaaataagaa gcgtgtacct tctttcatga attttgcaaa gtctaaacgc
     1921 agaccaattg ccgctaaagc agtgatctca aaccagctgc tgataaaatg tgccgattgg
     1981 tcaataacta caggtaaaga aaacaagctg ttgaccaaac aaaagatcac aaaccctaat
     2041 acataccaag gaagtggtaa ttttcgtttt ttctgcgtat cagttggcgt ttcaaacaga
     2101 gctggttgtt tcttgtgcat gaatttttca aacagataga caacaacaac gagcatcata
     2161 atacgtagaa tcttaaatag catggcgaat tcgacaacag aattgtcaag taagcttgcc
     2221 ccagcgacta cttgaccaac agattggagt gtgccaccca aaagtgcact tttttcaatg
     2281 agatttccac caaacaactg taatccaaga aaagggagag tcaacatcat cacagtaccc
     2341 agtagattga ctagtgtgat gatctgtcct ttttcttcct ctttcgcatg gatggaaggg
     2401 gcgatcgaag caatagcaga agaaccacaa acagcgtttc ctccagccat catcaaagaa
     2461 gaagtatcgc tgaattgcag ttttttacca atcaagtaag caccgatgat agtgactgac
     2521 atttgcagta acacaaaaac aattcctttt ataccaattt gagcaatcgt ttgagctgtg
     2581 acagttgcac ctagtaaaaa aacagagaat tctaatagtt tgctttcagc aaattttgtg
     2641 ccgattgcta gaacaggctg tttaaaaaaa gtatttccta ataaaatacc caataaaata
     2701 gcaatagtcg ctccacctaa ctgtggcaga taaatagcaa gaagcctgct gaaggcagcg
     2761 atggcaaaag aaagcagtaa accgggtaag atttcgcgga ccgctgaatc ctttacagat
     2821 tttattggat gtgacaatga tttcatttga tcggttcctt tcgtcaataa atactaaaag
     2881 catactcgaa catagtagca ctaaaccagt attttgacaa aagaacaaga ggtcgtgaca
     2941 aaagtcttgt cacgacctct tgtttcgaat aaacggtgtt caaaagccag tttctcggta
     3001 ttaagccaaa tttatgcaaa aatgtggaaa gccattttca caaaattttt cttaatactc
     3061 gaaactaagc ggctttttca cgacctcttg gtaaacggtg ttccagaagt agcttcttcg
     3121 gaaataagcc gaaatttcac aaaaatttga gaagcaattt tcgtaaattc cttcttattt
     3181 cttgaagctg accacttctt ttacgacctc ttggtaaacg gtgttcaaaa gcttgcatct
     3241 gcggtaataa gcccaaccag tacaaaaaca tgaacagctg tttttgtttg gccgttctta
     3301 ttacttgatg cagaacagct ttttcacaac ctattgaatc gattattaag cgattttttt
     3361 agaaacaaat tttccatgtt tgctatgttc tttaattaag aaacgtacgt aatgagcaag
     3421 aacgtagaag acaactacac ttgcaaacag acattccgca acttttaaga aatccaatac
     3481 gctactatag atacctaaac tgttgaagta atgaacagta gtaaatatag ctgttgcaga
     3541 gataacaagc gggaatgtaa aggctgcata gcttggataa aaacgaagct tgaacaaacg
     3601 tggaagcata gacagcacaa cgaaatacaa tacttgagat aaagcgaaca atgctacgac
     3661 aagataaaaa ttaggttcag caaagttttt aagataacct gttaggcaaa gagaaccagg
     3721 tgctgcaacg atcgtaatca gcggacgtgt cggatgagcc atatttctta cgaaaaagac
     3781 acgtttgatg atgatcggca ataagaacgc atagcagatc aatgaaatcc aaaagaaaat
     3841 ttttccaata agcgggaaga agttcccggc agttacagtg atgaccccaa atccaacgta
     3901 aatgataaac cagcttggat aaattgcttc aattgaaaga tcagctttga taacatggtg
     3961 ataattgaaa taaaagacaa ggatcacttg gaagataaca gcgcacagcc aaatgaattt
     4021 gacaattggt gcaatacttt catatgaaac aaaataagta caaataacca tgagtgacat
     4081 cgtaaaagtt ggtgaaacgg aggcgatgat tggatcatgc aacgtttgtt tcgtgtgttc
     4141 gaataagata aacaatttaa caaaaatgaa tatcataaga atcatagaga taccgccagt
     4201 tagattgcct aaagcaacat ggtgatagtc tttaaacaga tttccaaggg aagctaaccc
     4261 aaggatcaac ccgcagatcg ggatcggaat aattttcaaa aagttcttca tatataatat
     4321 tctccttttt atttaataga ttttagtacc aagatgaacg aatacattgt aaaatgaata
     4381 ggaatgtatg attacgagca agcagacagc tgattgtctt catctaagtt actagttgga
     4441 tcagcaactg ttttgttgta catttctatt atagatggtt ttttgtatat ttaaatcaaa
     4501 tagtttttat agtaattatc ataaaaagtt atggaagtgg tgccatgttt aagttattaa
     4561 cgacttttcg agcagtatat gaaacaagaa atttttccag agcagcagaa gtactgtttt
     4621 tatctcaacc agcagtctcg aatcaaatca aacagttgga gcgggaattg aatatcgatc
     4681 tttttcaacg taacggaaga aaagagatgc ttccaactaa gcaagccgac ttattatatg
     4741 aacggacact ggttttgtta gaagaatggg aagaaactca gcaaaaattg ctcaatgaac
     4801 aaaatgaaga agaagtatgt cggatcgttg cttcccacac ctttgctgtc tatcttttgc
     4861 ctcagttgat gaaacatctg atcaaaaggt ttcctaaagt gcattttgag atcgaaatcg
     4921 cgaattctca tgaatcatta gaacaagtag cgaaacatga atccgatttt ggttttatcg
     4981 aaaagccatt agagacttcc ggcgtacaaa gatactcttt gatggaagac cagctagtca
     5041 tcgctgggga cccaaaaagc aaattgtggc ttgtaagaga agcaaactct ggtgtttttc
     5101 attatacgca gcgttatttt gaagagatga atatccaaga taagaagatg atcgtgaaaa
     5161 gtaacgatgt aattgttgcg ttgctgaagg aaggaatcgg tcgctcgatt ttgtctaagc
     5221 gctctgttcc agaaaatgtg ccaatgactc cgttgaattt ccatcgatat ttttatttca
     5281 tccaacgaac caatctggtt tctgatcagc tgctagagat tgccgaagag atccttcgat
     5341 acaatgaaag cacagaaact tctcacagcg aggagaatct cagctggtag ataattgcat
     5401 gtatcaaaaa aagttctatg atacactaaa gataagcagt aggcttttgt actgcatttg
     5461 ctcaagcaac tgcacaaacg gaaagttaat ttttcccaaa tgtctatgga aagactttcc
     5521 attatctaac tggaccgcta taataatcag cagtatggtt tttaagaaaa gggatgaaac
     5581 atggaaaaaa aattaaaagc ttcgaacgat aatcgtattg attatggcgt gattttacca
     5641 gtctttctgt tatgtttgat tggtctgctc tcattatatg tagcacttag ccatgatccg
     5701 aacaatccag cagtcttgaa aggcgtaggg atgcaagttg tctggtattt agttggagcg
     5761 gcagcaatcg ttgtcatcat gcatttgaat tccaaatgga tctggaaact gactccttac
     5821 ctttatggtt tggggttagt ggtaatggga ctcttgctca aattctatga tcagaattta
     5881 gctgcttcaa cagggtctaa aaactggttt cgattcggtg cgtttacttt ccagccagca
     5941 gagttgatga agattgcgta catcttgatg atggcactga tcgtaactaa acataatacg
     6001 caagtaaaag aacggacgat gaaatcggac ttttggctga ttgggaagct cttgatcgtg
     6061 acaataccgg ttttagcatt gatcatggcc caagatgact ttggaacgat gcttgtcttt
     6121 cttgcgatct ttggcgggat tttcttgatg tctggaatct cttggcgcat catcgcacct
     6181 gtagtaatct tggcagtagt cgttggagca ggaacgatct tcttggttac gactgaaggc
     6241 ggaagagact tgctttacaa agttggcttc aaatcttatc agtttgcacg tattgattca
     6301 tggctggacc ctttccatga tacgtcagga atgagttatc agccggccca aggacttttg
     6361 gcaattggta ccggtggctt gtttggtaaa ggattcaacg taagcaatat ttatgtgcct
     6421 gttcgtgagt ccgatatgat ttttaccgtt atcggtgaaa acttcggctt tatcggtggt
     6481 gcattcgtca ttttcttgta ttttatttta atctatcgta tgattcgggt gtgtttcgat
     6541 acgaataatg aattttacgc ctatatcgca agcggtttga tcatgatgct gctgttccat
     6601 gtctttgaga atattggagc aaatatcgga ttacttcctt taacagggat cccattgcct
     6661 ttcattagtc agggaggatc ttcgatttta gggaacatga tcggtatcgg gttgatatta
     6721 tcgatgagat accaaaatga agcacctgca agatcttcaa ggaggtaaga aaattgtaca
     6781 ccttttactg gtacccgaaa tgttcaacat gcaaaaaagc caaagcgtgg ttagaccagc
     6841 atggcgttag ttatcaaact gttgatatga tcaaagaagt accgaaggcc tcaacgattg
     6901 agacttggct agaaacgagt caagtaccaa tccgtcgcct gttcaacaca agcgggatga
     6961 aatatcggga acttggactg aaagatcaag taggcgagtt tacggcttca aaagcgagcc
     7021 aagtcttatc gacagatggt atgctgatca agcggccact gatcttacga gaggaccagc
     7081 ttgttgcaat tggattcaat gaaaaaattt atgaaggaga actgatataa aatggcaggc
     7141 aagcatttga agaaaaagga caacctttgg atcttattta atggaaaaga atactgtgtt
     7201 ggtttgacaa atgaagcgca agaagaactt ggagacatca cgtttgccaa tctgccaaaa
     7261 gcaggacaat cgctaaaaaa agatgaacct ctgatcgaag ttgaagcgga aaaagcggtc
     7321 aatgaatttg ccagcccgct aacgggtaca gtttcttctg ttaatgataa aatcggtgaa
     7381 gatatcaata tcttgaatga ttcagatgaa atgaatgcgt ggatcttaag tttcaaagat
     7441 gttgatccaa aagaatttga cgaattataa aatcagaaaa tcacttgaaa aaagttgaca
     7501 ctgattcttc cagttgctat aatcataagc aatcctatat gaaacgagaa agctttgaaa
     7561 agaagagtac atgtcacgga cgtttgaaga gagccctgtt tgctgaaaat gggtaacgaa
     7621 aatgagatgg aagatggtct tagagcagag taagcgagcg taagcaaact tactacggga
     7681 gtgcccgtta cagcgcgaca gtatacggaa agaagctaag tccaagtact ggaagaggct
     7741 gttattgcga agtaacggta aatcaaggtg gtaacacgga agttcaagct ttcgtccttt
     7801 tgcttaaggc aagagggcga aggctttttt gtttgaaaaa tcataagaga aagatgtgaa
     7861 tggcatgtca ttaatccaac taacgaatgt caaaaagaca ttcaatggga aaaacggaaa
     7921 catccaagcc gtcaatgacg tttcactaaa cgttgaaaaa ggggatattt atggaatcgt
     7981 cggctattct ggcgcaggaa aaagtacatt agtccgtctc ctgaatggac ttgagcttcc
     8041 cacgagcggc gaagtgatcg taaacgatca agatattaca aaattaaaaa acaaagaatt
     8101 acgtacattc cgaaagaaaa tcggtatgat cttccagcat ttcaacctgt tatggtcaag
     8161 aactgtactt gaaaatatcc agctgccgct ggaattagct ggcgtaccaa agagcaaacg
     8221 taaagaacgg gcagaagaac tgttgcgttt agtcgggtta gaaggacgcg gacaagcata
     8281 tccttcacaa ctttccggcg gacagaaaca acgcgtcggt atcgcacgag cattggcaaa
     8341 tgatcctgag atcctgcttt gtgatgaggc tactagcgca ttggacccgc aaacaacaga
     8401 agaagtatta gacttattgt tagcgatcaa taagaaattg aatttgacca tcgtcttgat
     8461 cacacatgaa atgaatgtga tccgcaaaat ctgcaacaaa gtcgctgtga tggagttggg
     8521 gaaagtcgta gaagaaggag acgtattgac tgtcttcaga caaccgaaaa aagaagtaac
     8581 caaacgcttt gtacaacaag acatcgaacc agaagctgat tcgacagaac tattagctga
     8641 actcatcaaa gaaaatccaa gcggccgttt ggcaactttg acctttagcg aaagaaatgc
     8701 caacgagcca gtcatttccc aagcgattcg acgctataac atcgatgtca acgttgtgta
     8761 tggaaagatc aaacaagtga aagaaggttc ctttggttca ttgactgtct tgatgactgg
     8821 tgaaaccaaa gaattagatc aagcagaagt gttcatcaaa gaacaaggag taggaattga
     8881 ggtgatccat cgtggataaa agcttttcag aaacatatct agattttaaa caaatcaacc
     8941 cggaaacgat gaatcaggct atccttgata cactatatat gacattaatc tcaatggtct
     9001 tagtgacgat catcggatta gttttgggat tgatccttta ctctattgga cgcaaaaaca
     9061 aaccgtatgc tcgaatcatt tatggaatcg tatcgatcat cagtaatatt ttccggtcaa
     9121 cgccatttat gatcctgatg gttttgatca ttccatttac taaagcaatc gtaggcacta
     9181 tgctaggtgc gcaagcagcc attccagctc ttgttttatc cgcagcacca ttttatgccc
     9241 gtctagtaga gattgctttt cgtgaagtca gccctggtgt actggaagca gcagatgcaa
     9301 tgggagcaag ttactgggaa atcatctgga aggtcttgat acctgaaagt attcccgcat
     9361 tgatttctgg attaacagtt acaacgatct cgatgatcgg atttactgcg atggccggcg
     9421 cgatcggcgc aggtggactt ggaggtcttg cttggcagga aggctaccag cgtggtaatc
     9481 tgacagtgac attcgttgcg actttaatta ttttactaat cgtatttatc gttcaaggaa
     9541 tcggtgattt cttaactaag aaaacagaca aaagataata ggatataggg ggatgtagga
     9601 tatgaaaaag aaaatttttg gatttgcagc agcgcttttg ctgactgtag gattagcagc
     9661 atgcggtaac aacagcgaca gcaaagattc agcaaataaa gcagacgaca cgacattgaa
     9721 agtcggcgct tcaccgacac cgcacgcaga gattttggaa catgtgaaac cattattgaa
     9781 agatgaagga atcgatcttg aaatcgtcaa attcgatgac tatgtattac cgaaccaatc
     9841 tttggaagaa ggcgacattg atgccaatta cttccagcat attccttatt tgaacaaaga
     9901 aatcaaagaa aaaggatatg actttgtcaa tgccggtgct gtccacatcg aaccaatggg
     9961 cttgtactct aaaaaagtca aagatgtttc cgaattaaaa gatggtgcaa caatcattac
    10021 ttcaaattct gaatcagact ggggacgcat catcacaatt ctacaagatg ctggcttagt
    10081 caaagtcaaa gatggagtag atctggaaac agcaacattc gatgatattg aagaaaaccc
    10141 taagaacttg aagttcaacc atacgatcga cccatcactt ttagcttcta cgtaccaaaa
    10201 tgatgaaggt gacctagttg caatcaacgc aaacttcgct tacggcgcag ggttgaatcc
    10261 attagaagat gccgtattgc ttgaaaagga caactctcct tacgtgaata ttattgcgac
    10321 tcgtaaaggt gatgaagaca gtgacaaaat caaaaaactg attgaagtgc ttcaccgcaa
    10381 agaagtccaa gaatggatca cagacaaatg gggcggctct gttaaaccag taaaggctga
    10441 tgcaaaataa ggtatggaac aaaaaagatg agaaataagt ctgtaatggc ttatttctca
    10501 tcttttttat acctattcta atttagaaat caaaattgtt taataataaa taaaagatat
    10561 ttgattttag taaggaaggt cattgcgctt cattctcaat tagtctaaaa tgagtgagaa
    10621 cgaaagagaa tttgggggta taatgcatgt ctgtcttaga aataaaaaat ctacatgtgt
    10681 cgatcgagga aaaagaaatc cttaaaggcg taaacctaac aatgaaaacc ggagaaatcc
    10741 atgccatcat gggacctaac ggcacaggaa aatctacctt atctgccgct atcatgggca
    10801 atccaaacta tgaagtaaca gaaggcgaaa ttttgtttga cgggaaaaat gttttagact
    10861 tggaagtaga tgaacgtgcg cgacttggct tatttttagc tatgcaatat ccaagtgaga
    10921 taccaggtat cacgaatgcg gaattcatgc gtgcagcgat caatgcgaaa cgtgatgaaa
    10981 acgacaaaat ttctgttatg gcatttttga aaaagttaga tgaaaaaatg gctttactaa
    11041 atatgccaga agaaatggct gaacgttatc tgaacgaagg tttttctggc ggagaaaaga
    11101 aacggaatga gatcctgcaa ttgttgatgt tagaaccaac atttgcaatt ttagacgaaa
    11161 tcgattcagg tctggatatc gatgctttga aagtcgttgc taaaggagta aatgaaatgc
    11221 gcggagacaa tttcggtgca ttgatcatca cccactatca acgattattg aactatatca
    11281 cacctgatgt cgttcacatc atgatggaag gccgagtagt catgacaggg aatgctgacc
    11341 tagctaaacg tctggaagca gaaggctacg ctggtatcag taaagaatta ggaatcgact
    11401 ataaagaaga agaagcgtaa gggggagcaa gaatgaaaga aaaaaactgg ctcgattatc
    11461 ttgacgcggt aaatgatttt tctttaagta aaggcgaacc tgattggatg agaaccttcc
    11521 gtcaagacgc actagcgaaa gcagatgaac tgccattgcc gcatatcgat cgggtgaaat
    11581 tccatcgctg gtcgttattt gatgtgaaag aaacacaaac catcagtgaa acaggcacga
    11641 ttccggcatt tgatgcaatg aaagacaatc ctgtgcttgt ccaacaaggt tcatggacga
    11701 tttttgaaca actgcctgtg gaattagcag aaaaaggagt catttttacc gacttgttca
    11761 ctgcaatgat cgaatatcca gaactagtcc aagaatatta tatgaaaaaa gcagtaaaca
    11821 tgaacgaaga ccaattgact gcactccatg tagcttttat gaacagcggt attttcctgt
    11881 atgtgcctaa aaatgtcgtg atcgatgaac cactcgagtc cttattcatt caagatggcg
    11941 catccgatga gcacttcttc aagcatgtgt tgatcgtggc agatgaacat agtgaatttt
    12001 cttatctgga acgtttccag acaaccaaag aacaagtagc aaaatcatca ggtaatatca
    12061 tcgtggaagt catcgctaaa gctggttcaa aaatcaaata ttcagccgta gatcaattag
    12121 gagaaaatat tacttcttat atgaatcgcc gtgggcacat tttacgagat gcttctgtcg
    12181 actgggcaat tggtgtcatg aatgacggtc atgtgatcgc tgactttgac tcagatttag
    12241 caggagaagg tgcacacgca gaagtcaaaa tcgtggcaat cagctcgggc agacaaatcc
    12301 aaggaatcga tacgcgagta accaataaag cccctcatac gatcgggcat attttgcaac
    12361 acggagtgat ccgagaaaaa ggaacattga cctttaatgg tatcggccac atcttgaaag
    12421 gggcaaaagg tgcagatgcc caacaagaaa gtcgtgtctt gatgctttct gataaagcac
    12481 gtggtgatgc gaatcctatt cttttgatcg atgaaaacga agtaactgct ggacatgctg
    12541 ccagtgtagg acgagttgat ccagaagaaa tgtactatct gatgagtcga ggattgcata
    12601 aagaagaagc agagcgctta gtgattcgag gattcctcgg ttctgtctta acagcaatcc
    12661 ctgtagaaca agttcggaaa gaattagtcg aagtaatcga agggaagttg aatggatgat
    12721 cgatgctaaa aagctacgca atgattttcc tatcttattt caagacgtca atgatgaacc
    12781 tttagtttat ctggataatg cagcgacgac ccaaaagcca aaacaagtac tccaggcatt
    12841 agcgcactat tacgaacacg ataacgccaa tgttcatcgc ggtgtccata cgttagcaga
    12901 acgcgcgacc catgattatg aacaagcaag agaaaaagta cgggcgttca tccatgcaaa
    12961 agaaacagca gaagtgctgt ttactcgtgg tacgacgaca agcttgaatt gggtagccag
    13021 aagcttcggc gaccgctata tcaaagcagg agacgagata gtgctctctt acatggaaca
    13081 tcattctaat gttatcccgt ggcagcagtt ggcaaaacga acaggtgctg tcttgaaata
    13141 tatcgaacta acagaagaag gcttcctgga tatggaggat gcagcgaaaa agattacgga
    13201 caaaaccaaa atcgtctcca ttgctcatgt atccaatgtc ttaggtgtca tcaatcctgt
    13261 tcaagaatta gctgaacttg tccatgaaaa cggcggggtc atcgtagtag atggtgctca
    13321 atctgccccg cacatgacca ttgatgttca agcactagac tgtgatttct ttgctttcag
    13381 cggacacaaa atgtgcggac caacaggtat cggtgtttta tatggtaaac gaaaatggtt
    13441 agaagaaatg gaacctgttg aatttggtgg cgaaatgatc gattttgtcg aattatacga
    13501 cagtacatgg aaagaactac cttggaaatt cgaagcaggg acaccaaata tcgctggcgc
    13561 tatcgcatta ggtcatgcaa tcgattattt gcaagaactc ggcatggaca atatccatcg
    13621 atatgaagaa gaactagccg cttatgtgtt gccaaaatta caagcaatcg atggattgac
    13681 tgtttatggt ccgcaagacc cgaattttcg gacaggagtc attgcgttca atttggatgg
    13741 gttacatccc catgatgtcg cgacagcttt agacatggaa ggggtagccg ttagagcagg
    13801 tcatcattgt gcgcaaccgc tattgaagta ccttgaagta tcagcgactg ctcgcgccag
    13861 cttttatttc tacaacacaa cgcaagacgc cgaccgttta gttgaagcaa tcatggcaac
    13921 aaaggagttt ttcaaacatg ggactatcta aattagacaa tttataccgt caagtgatct
    13981 tggatcattc ttcccatcca catcatcgtg gaagcttgac tgactcgaca agcaagatcg
    14041 aattgaacaa ccccacatgt ggagatgtga tcgaattgca agttgatgta aaagatggaa
    14101 tcatcaaaaa tatcgccttt gacggcagcg gctgttccat cagtacagca agtgcctcga
    14161 tgatgacaga tgccgtcatt ggaaaatcaa tagaagaagc agaacattta actgtagact
    14221 ttctacagct tgtccaagga aaagaagtca aagatcccga ccaattaggc gatgctgcta
    14281 tgcttggtgg tgtagctaaa tttcccgctc ggatcaaatg tgcgacactt gcctggaaag
    14341 cactaggaaa agcaatggaa aacgatggta ccgcagcttt ccatcattac cacaaggaag
    14401 aggagtgaga acatgggcgt accagaatta gaagaatata aattcgggtt tcacgatgat
    14461 gtcgaaccgg ttttcagtac aggagaaggc ttgacagaag aagtgatccg tgagatgtca
    14521 agaatcaaag gcgaaccaga atggatgctt gaattccgtc tgaaatctct agaaacattc
    14581 aataaaatgc caatgcaaac atgggggccc gacctttcag acatcgattt cgatgccatc
    14641 aaatattacc aaaaaccaag tgacaaaccc gctcgtgact gggaagacgt accagaaaaa
    14701 atcaaagata catttgaaaa gattggtata ccagaagctg aacgagctta tctagccggt
    14761 gcttccgcac aatatgaatc agaagtcgtg taccataata tgaaagatga attcgaaaag
    14821 ttaggaatcg tctttactga cactgactct gctttgaaag aataccctga attattcaaa
    14881 gagtactttt caaaacttgt accgccaacc gacaacaaat tagcagcgct gaattcagcc
    14941 gtatggtcag gtggcacctt tatttacgtg ccaaaaggcg tcaaagtcga tgttccatta
    15001 caaacgtact tccggatcaa tgcggaaaac acggggcaat tcgaacgtac gctgatcatt
    15061 gttgacgaag gagcaagcat ccactatgtc gaaggttgta cggcacctac ttattcaagt
    15121 aatagtctgc atgctgccat cgtagaaatc tttacaagaa aagatgcgta ttgccgttac
    15181 accacgatcc aaaactggtc agacaatgtg tacaacttag taaccaaacg agcaaaagca
    15241 tacgaaggcg caaccgttga atggatcgac ggaaacttag gtgcaaaaac aacgatgaaa
    15301 tacccaagtg tttacttaga tggaaaaggt gcacgaggca ccatgctctc catcgccttt
    15361 gcaggagcaa accaaatcca agatacgggt gcaaaaatga tccataatgc accaaataca
    15421 tctagctcta tcgtctctaa atcaatttca aaagacggcg gagaagtaaa ctatcgagga
    15481 caagtaacct ttgccaaaaa cagtgcgggt tcgatctctc atatcgaatg tgacacgatc
    15541 atcatggacg aatggtcgaa atccgatacg atcccattca atgagatcca caacagccaa
    15601 gtctctttag aacacgaagc caaagtctca aaaatctcag aagaacaact ctactatctg
    15661 atgagccgag gcctgaccga atctgaagca actgaaatga tcgtcatggg ctttgttgaa
    15721 ccattcacca aagaacttcc aatggaatat gcagtcgaac tcaaccgatt gatcagttat
    15781 gagatggaag gcagtgtagg ataaacttgt tataggagct agaatccttg gtatataagg
    15841 gttctagctt ttttattttg tctatatact agaaatgacc agcttttgac cagctctttt
    15901 actgatgagc catcatatca gcaaagtctt gacctgtttt cttaggtgca ttatttgtaa
    15961 tatgtgcata gatgtctaat gtcactttac tattttcatg ccctaatcgt tgttgaacta
    16021 ccttgacaga ttctcctgct tctaataata aactagcatg tgtatgtcta aacccatgag
    16081 gtgtaatacg aggcaagtta tattttttca aaatatatct taaccaatca ttaacagtgt
    16141 ttggaatgta tagagtgttt ttaactgttg taaaaacatg ttgctcttta gagctagtat
    16201 tgtatccaag ttttaaatac tcttcttttt gctgtaattt ccagttgttg aggatagaaa
    16261 gagtctcagt atctaaagag ataactcgtc tagaagctct tgttttcgga gattgaatta
    16321 taactttacc aaattcatct acagcaagag ttttattgac atgtagttct ttattaaacg
    16381 tgtctatgtc tttccactgt aaagcgagta cttcactctt tctcatacct gtgtatgcca
    16441 ataatctgaa gaaggcgtat tgtttcatgt taccaaaatc tttaaaagca tccagtaaat
    16501 tattaagctc ctcttttgag taaaatttac gatttttctc atactctttt tttcgtggaa
    16561 gaagtgtttt ttccatagga ttattgtcaa tatattgcat tgcaaccccg tagcgtaaaa
    16621 tttgtgcagt ctgttttctc atataatcgt aactttcata tttactgtgc cattcatcta
    16681 ccactttttg acaatatggg acagagatat tcgatagttt aagcttacca aatgctggaa
    16741 gtatttgatt atttgcgtat cttactgaga ttgcgattgt tgaagcttta acactggtac
    16801 gctgatgagt tatccattca ttatacaggt cttcaaatgt catatcaggt gctttctttt
    16861 taacaccgta taatatttga tgtctgtact cgtcataatc tttttgagct tcttgtttgc
    16921 tactaaaacc tcttctcgta gtatttattt tttcaccagt tcgtggatcg gtggcaatgt
    16981 gtcctttata catccacgcc acttgaccgt ttttcttttt gtactttgat atttttgcca
    17041 tgaaatcatc tcctcttagt ttacaaggag tacttaccac tagatggtat cacctccctt
    17101 catttttttc ttagtgtatt ttttcccaat tgatgaaatc gtttgtttta gcttgtttat
    17161 attatcaata atttcgttta cagcttcttc gggataattc atgtcttcca cttgactatg
    17221 tatcccttca tctatcattt cttctagttg ttcctctata ctgctagtga gcagaagaat
    17281 gttattgtca aattcttcta taatagcatt gatgaataat tcttctattt ttaagatatt
    17341 gaaaagacta attttttcat ttttaacttt attacaagtc ttgttagcaa taaattcttg
    17401 catctctggt tctaatttgg aatatctatc cagccatgta tctaaattag agtgtactgg
    17461 tgggaactgt tgacgaagat gctggaacag gtttttatct ggaaatgtta taccatctag
    17521 tacaatgcta tctctataaa gaataattcc caaaatatat tcttctacat tgccacaaac
    17581 taactcctct atactcatat ttcccaaatc agcaattcgg gataatcgct ctggtattgg
    17641 taaataggtt cccttttccc attcgtagat agtttttttg tcaatcaatt gaccattgtt
    17701 aatcataaga ccaaatgctg gttgtgttaa ttttttactc tttcttatag cttttatttt
    17761 tgttccgagt atcttttttc gttctaaata atttattggt ggacgcttta tcatattgac
    17821 cacacctcct aaaattagta tacctcaaaa agggggtgtt gtaaataaat tcacacaaca
    17881 tattgacacc ccttttgtat cgaggtatta tgtgtatgtc gatgaactga taatcatttt
    17941 ttatttgatt aatcggttaa taacaacaaa atacttttat tttgatcaaa taatacgaag
    18001 atgattctta gaaaatttgt tcaagtaatc acgaaaggag ggggaaatat gtctctacaa
    18061 atcattgata gcattgtttc tatacaagtt gaacaacaag tgaaagagat aattacagtg
    18121 ctaaagccta tgctagaaga cattatttct ggtgttactc tagagtatat ggactatcag
    18181 caggcaagtg aatatctagg tgtatcagta ggaacgatta gaaaatatgt ttctcaatac
    18241 gggttacctg ttataaaaat tgatacggtc attagattca aaaaatctga tattgatagt
    18301 ttcatggaac agtataaagg ttgagtaaac aaataagact aaaacatata gtaataaaga
    18361 gtttgtgttt attatctgta tgttttagtc tttaacagaa ttttataagg aggaaacaca
    18421 aatggaaaca tcaatgatta atattccaat ttatgtagat caagaaaaat tattagagaa
    18481 gccagttaca aaaaaagaaa aaactgatgt aagagatagg tgtttagata actatagaga
    18541 agttacaaga gatgagtttg tagagatact aaattcatca caatcattta ttccaagcaa
    18601 gacaaagaat aaaggcaatg acaaggagag ttttgtggaa accagagtaa ttattctgga
    18661 tgtcgataac actgttaaag atgagaaggg gaaggtagta gatttgagta aagatgatag
    18721 tagatactta agcgtagaaa aggttttatc tatcaattta gttcataatt ctgcgtttgc
    18781 gatacaaaaa agtattcggt actctgaaaa tctagaaaaa tacaagattg tctttctatt
    18841 aaaagaagca attactgact atggtaaaat ggttgctgtt tacgaatact tgaaaaagaa
    18901 catccccggg tgtgatgaca atgtaaatgc ttcgaataga atgttttttg gaggatataa
    18961 gtcagatgca gtgataataa ttaatgaaga caacatgtta gatattactg agcttccgat
    19021 tgattttacc atgacatgtt ctaaaaattg tcaaagtgat tcagggagag agtatgaact
    19081 agtcaatgaa accgattttg ttaagttgat aaaaaatgac gacaaagaag aaatgaaaca
    19141 gtggtttaaa gactgcctgt ttgattcctc cgacatgtct tttaatgaga tttatgaaag
    19201 attgttaacc attgatatga ataaattact taagtcaagt aaaaacttaa gatgtctgtt
    19261 tcacaacgat cataatccct ctgctagtat ttttaccagt aaaactgggc attccatttt
    19321 ttattgtcat tccagtaatt gtgatatcag tgaaaatttt attggagttg tgatgctaat
    19381 tttaaagaaa gacagtcggg tagaaacatt taattggcta ttagagaacc tagacttgta
    19441 tcctacacat tttcgaaaat tgaaagagga atcaaaagta ctttttgaca ctcttgaatc
    19501 aatgaaagac aaacttttca ggacaataag gaattatcta gaggaaatgc gacgtgtcta
    19561 tgatgtcttg ctttctgatg ttaatttttt tgatgatagt aaggaaagtg tgacttgtat
    19621 tttgtcgggg gaacaattag ctaaaaaaat gagttcttac tatggtaa
//
LOCUS       DAZKKW010000049        19646 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.049, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000049 DAZKKW010000000
VERSION     DAZKKW010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19646
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.049"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            133..3660
                     /locus_tag="ACG8PO_001698"
     CDS             133..3660
                     /locus_tag="ACG8PO_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HFQ0207010.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3657..7379
                     /gene="addA"
                     /locus_tag="ACG8PO_001699"
     CDS             3657..7379
                     /gene="addA"
                     /locus_tag="ACG8PO_001699"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HFQ0207011.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7448..7789
                     /locus_tag="ACG8PO_001700"
     CDS             7448..7789
                     /locus_tag="ACG8PO_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFQ0207012.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8098..9141
                     /gene="pheS"
                     /locus_tag="ACG8PO_001701"
     CDS             8098..9141
                     /gene="pheS"
                     /locus_tag="ACG8PO_001701"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFQ0207013.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9146..11566
                     /gene="pheT"
                     /locus_tag="ACG8PO_001702"
     CDS             9146..11566
                     /gene="pheT"
                     /locus_tag="ACG8PO_001702"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFQ0207014.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="ACG8PO_001703"
     CDS             complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="ACG8PO_001703"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFQ0207015.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            13000..13737
                     /locus_tag="ACG8PO_001704"
     CDS             13000..13737
                     /locus_tag="ACG8PO_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFQ0207016.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13751..14566
                     /locus_tag="ACG8PO_001705"
     CDS             13751..14566
                     /locus_tag="ACG8PO_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFQ0207017.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14579..15226
                     /locus_tag="ACG8PO_001706"
     CDS             14579..15226
                     /locus_tag="ACG8PO_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFQ0207018.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15242..15898
                     /locus_tag="ACG8PO_001707"
     CDS             15242..15898
                     /locus_tag="ACG8PO_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFQ0207019.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16060..16881
                     /gene="racE"
                     /locus_tag="ACG8PO_001708"
     CDS             16060..16881
                     /gene="racE"
                     /locus_tag="ACG8PO_001708"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFQ0207020.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16884..18239
                     /gene="rph"
                     /locus_tag="ACG8PO_001709"
     CDS             16884..18239
                     /gene="rph"
                     /locus_tag="ACG8PO_001709"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFQ0207021.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAEMIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18240..18758
                     /locus_tag="ACG8PO_001710"
     CDS             18240..18758
                     /locus_tag="ACG8PO_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFQ0207022.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18865..19365
                     /gene="cbpB"
                     /locus_tag="ACG8PO_001711"
     CDS             18865..19365
                     /gene="cbpB"
                     /locus_tag="ACG8PO_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0180001 - cyclic-di-AMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP-binding protein CbpB"
                     /protein_id="HFQ0207023.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
BASE COUNT         6587 a         3374 c         4215 g         5470 t
ORIGIN      
        1 tttttgcgta tacttaaaaa agtagaaata tcagaacctt aaaaggatat aatttgaaga
       61 atatagaaca aatgttcagt attttttgat ataataaagg agcaccgtat catgtgttga
      121 aggaggaaaa gcatgtcttt acaatttatt cttggaaacg ggaaaaaaga tcaccgtcaa
      181 gtcttgatcg acgaagcatc cgcttggctc gaccaggatg caagtaacca agtatttttc
      241 cttgttccca actacagcaa attcgaacag gaacaagaaa ttttagcgga gatgcgaaga
      301 agaagcggaa aaaaaacttt cagcacaaca aacgttcaag tatttagttt ttatcgtctg
      361 gcttggtatt tgctgcaaca gacaactctt cttacaggaa atgaattatc agaatcagga
      421 tctgcaatga tcttgcgaca gattttagaa aaaaatgagg aagaattaac aatctttcgc
      481 ggagaaatca ataaaagagg gtttattcgt caattacaag aactctacag tgaattgcgt
      541 acaggaaata ttcaaccaga agacctgatg cttttatttg gtgattcacc taatgcagaa
      601 gaccagcaac ttaaaatgaa agacttgaag ctgatttttt cagcttatga cagcgaattg
      661 actcaacgtg cactaacaaa tgaagatgca ctatctatac tagcaaatta tatgcagaca
      721 caagatttca acaacgtcaa atttattgtc agtggttttt ctcgaatcaa tggtcaagag
      781 tatcaattac tgcaggcaat gatggaaaag gggcaattgg ttgtcgattt attgctggat
      841 cgtccttata cagcggatct tccagaagta cttacgctct ttcatgagac tggaaagctt
      901 tatttccgtc tttttcaaag tgcaagagaa cggaaagtcc ctatattagt ggaccgattt
      961 gcaccagagc agactcgtcc gacagagctt caactgctgc aaacaatgtg ggaagagaca
     1021 tctagccaaa agaaagcggc agtacacaaa atagggggac aagagcatct ccatttgtgg
     1081 gaagctgaaa atccaactga agaagtccga caattagctg ttgaaatcag acgactagtc
     1141 agtgaagaaa actaccgtta tcgtgatatc caagtgctga caaaaaatat gtctttatat
     1201 ggaaatatcc ttcggccgat ctttcaagaa atggcaattc ctttttatat cgatgaagaa
     1261 caaatgatgg aaaatcatcc gctgatcgaa tggatcaaca gtctgttcgc tcttgaccgc
     1321 tattcttatc ggctaagtga tattatgcgt tttttcaaaa ctgaattatt tctgtcgacg
     1381 gaagcagaaa ccgaacttga tgaatggaaa caagagcgag atgaattcag gaaaaaactg
     1441 gacatcactg aaaatgtagc acttgcttac aattatcaag gaacttattg gactcgtgaa
     1501 aaagactggc agtttgtggc gtatgatttt gaagcagaac agattgaaga cgtacagtat
     1561 ttggaagaac agtcaaatac gattagacga ttatttcaga aagctgtacc agtatttttg
     1621 aaaaaaataa agaaaagcaa aacaggacaa gaggccgcaa atgtttttta tcaatttttg
     1681 atccaaagtg gcgtacagca acaattattg ttttggcgta atcaggaagt agacagagga
     1741 aacttagaag cggcaagaaa ccatgagcag acatggggag cgctgattga tctgttggat
     1801 gaatatgtag taatttatgg tgaatcggct tttgactggt cactgttcca agaaatcatt
     1861 acaagtggtc tagaaaatct aagttatggt aaaatcccta ccgcaatcga ccaagtgcga
     1921 atcaatcgtt tggaattagt tcgaccaaat caagctgccg tcacttttgc attagggttg
     1981 aatgatcaag tgtttccaga ccgaaaagag acaaaaggat tgttgtccag tgaagaacga
     2041 gaagtattaa atcaggcctt gccagatgga cagtttttat tcgacccatc aaaggaaagt
     2101 atctcttttg agcctttcca agcctatctt gtttttcttt ccggaacaga tcgattatat
     2161 cttagttatg cacaaagcta tgacacagat ggttcgctga agatgtctcc ttatttgcgt
     2221 agaatcacag aatatttatc tgttcccgtc gagagaaaag agcatttgac gatcgaaagc
     2281 gaccctaatt gttatgtagg gacttacaga agcggaatca atacagtcaa taggatttat
     2341 cgattagctt tagatgaaaa aaaacctgta ccaaagcact ggcaaaagct caaagaatta
     2401 gtactgaatt cgtcatggcg aaattttgct ttacagattt ttgaaagtca ggaacattta
     2461 aatgttcctg tttcgatgcc agaaaaaatg gcggaagagc tttatggaaa agagatttat
     2521 acttcaattt cacgtatgga gaatttttat aattgtgaat acaaatactt catgcaattt
     2581 ggtttgaaat tgaaagaacg aactgtttat ggtctaactc cagcagcaac tggtgatttt
     2641 tatcatgaat cattggatcg tttcttcaag ctactgtttt caaaccaatt atccttagtg
     2701 aatatgtccg atgcgcagcg tagagacttt actgaaaaag tgctgcagga agtattcgga
     2761 gaacttcggt ttgagatatt ggatagttct gcaagaatga actatatccg ctaccagtta
     2821 ggacagacga ttcaaaaagt gacatgggct ttgcaaaaac agagcaaaaa tacaggcatg
     2881 catcctcttc agacagagat tctatttggt cagattgctg gagcaaaagg tatcacaggt
     2941 ctggaattac cgttgagtaa tggtggaaaa ctccatgttc gagggaaaat cgatcgaatc
     3001 gatgtagctt ctgaacaaga agatacttgg ctaagcgtcg ttgattacaa atctagcgga
     3061 cgttcattcg atgtcacaga agcttattat ggcatggcta tgcaattatt gacgtatctg
     3121 gacgtagcat tgatggatgc ggtacgtttg actggaaagg cggctgttaa accagcagga
     3181 tcatattatt tacatgttca taatcctgta ttgacggaga cagatgatgt agagaagaat
     3241 acattgaaaa aatttagtta tgatggcgtt tttgtcgatg atcccgctct tttagaagta
     3301 ctagatcatt cactggaagc aaaagaacaa tcactgatct atccagtgaa aaaaaatgcg
     3361 aaagatgaat accaaaaagg tagtttcagt aaagacaaat ttttcacgga agaagaactg
     3421 caactattca tggcgcataa tcgggaaaat atgcggttag ctggagatgc gattacttcc
     3481 ggagaaatca aactaaatcc agcttataaa gataaacagc ggattgcctg cgaattttgt
     3541 ccattcagaa gcatctgtac gtttgatgtg atgctgaaag aaaataatta ccaccgtctg
     3601 gagaaaatag ataaggaaga agcactgcgt agaatattga aaaggagtga agaagagtga
     3661 atcaaattcc tttaaaaccg caaaatgaac gattcacaga tgatcagtgg caagcgattt
     3721 ttgatcaagg agataatcta ttagtctctg cgtctgccgg atcaggtaaa actacagtgc
     3781 ttgttcgaag agtgattgaa aaactaaaaa tgggttttga tatcgatgaa ttattgattg
     3841 tgacatttac tgaagctgcc gcaagagaaa tgaaagaaag aattcaagag gcattacaag
     3901 aatccgtcaa tagcgaaagt gaccctgtac gtagacagca ttttactaag cagttagttt
     3961 tattgcccac agcgaatatc tcaaccttac atgctttttg cctgacagtt attcgacgtt
     4021 attattactt aattgatatt gatccagtct ttcgcatgtt aacagatgaa acagaaacga
     4081 tcttgatgaa ggaggatgtg tgggacgagc tgagagaagc actttatgca gaaaatgatg
     4141 aacgattttt ccaattaaca atgaattttt ctaatgatcg ttcagatgac ggcttgacga
     4201 atctggtgtt ttctctttat gagtttgccc gtgccaaccc tgacccccaa aaatggctag
     4261 aacagttaag cgataattat cgtttgcctg aggggctagc gaaatctcga ttatatcaaa
     4321 aacaaatccg tcctttagtt ttggcagata tataccaatg tgtacagtta tatgaacaga
     4381 tgactcagtt agcacaaggt gaaggattag aaaaaatgca tgagcaagta gctggagaac
     4441 aacagcagat caagaatatc tatgaagcgt tctcgcaaga tcggttagaa gaagcttatg
     4501 ctggtttgga acaactaact ttttcaactt ttaagagcag tcggaaagca gaattgaaag
     4561 aaatttccaa tgaagtcaag ggcatgaggg ataaagcaaa aaaactgatt cagcagataa
     4621 gtaaaagtta ttttcctgtt tccccaagcc aaatggaaga attaacggac aaagcactac
     4681 cgttagtcga agaaatgaca aaagtcacgc aaagcttcat ggacggtttc agtatgcgta
     4741 aacgggaaaa aggtgtgctg gatttcaatg atctggaaca tttagcgttg cagattctca
     4801 cagaaaaaac gaaagatgta tggctgccaa gtgaagcttc aaaacattat cgaaaaaaat
     4861 tcaaagaagt aatggtagat gaatatcaag acgtgaacca attacaagaa gccattttat
     4921 actggctaag agaacccgat gatacaaaag gaaatatgtt catggttggg gatgtcaaac
     4981 aatccatcta ttctttccgt ttagcagatc ctagtttatt tatcgggaaa tacgagaatt
     5041 tctcaaagaa agaaggcgga cgtcgaatcg ttttggctga gaacttccgc tctagaaaag
     5101 aagtcctttc ttttacgaat cttatttttg aacaactaat ggatccagca gtgggtcaaa
     5161 tcaattatga cgaagcagcc aaactgatcc aaggattttc agactttcca gagaatgaac
     5221 agtttgagcc ggaaatcatg atctatgaaa aagaacagga agaatcagag attgagattc
     5281 cgacggatga tatactggaa gacaaaacag aaggcgaact ttttatgaca ggactaaaaa
     5341 ttcgtcaatt aattgattcc tcctttatga tttatgataa aaaatcaaaa aaaagtcgtc
     5401 cgatagagta caaagacatt gttctcttga cacctacaaa gaagaacaac ttaacgattt
     5461 tagagatttt caaaacattg gatattccat tagagatgaa tgatgctcaa aactattttc
     5521 aagcgacaga aattcgcacg atgatctcgc tgctacaatt aatcgataat ccctatcaag
     5581 atattccgct tgcggctgta ttgcgctcac cgatcgtggg tctgattgaa ccagaattag
     5641 ccagcattcg attagctaac cgtgcacata cttactatga tgctgtgctt gcttatcaag
     5701 catcaaacga agatgaatta gcagctaagc tggaacattt tggcaagcaa ctagagcatt
     5761 ggcgagaact tgctcgccgt tcttcgatca ctgacttgtt atgggatatc tattatgaga
     5821 cgggatatct agagtatgtg gtcggtctgc cagcaggggc acagagacaa gccaatcttt
     5881 atgcattggt tgatcgtgcc aaagcttacg agcaaagttc tttcagagga ttataccagt
     5941 tcgtgcgttt cattgaaaaa atgcaagaaa aagataaaga cttagcagaa ccagtcattt
     6001 caatagaaga taatgctgtt cgcgttatga cgatccatgc aagtaaaggc ttggaattcc
     6061 cagttgtatt tttgttagat atgacaaagg agttcaactt acaagacttg cgcaatcggt
     6121 atgcttttga agaaaaactt ggagcaggaa ttcgctatat ggatcctgaa acacgagtgt
     6181 tatatgatac gttaccgttt caagccatca aattggcaaa gcaaaacaaa ttgttgtcgg
     6241 aagaaatgcg taaattgtat gtaggattga ctcgggcaga acaaaagctt ttcattgtcg
     6301 gttcctataa aaataaagag caaatgatcc aaacgtggtc tgaagcagct gatcatgagg
     6361 agttagtttt tgatcctgcg ctacgtttga agggcagaag cagcttgatg aattggattg
     6421 gttacggatt gatccgtcat cctgaaatgc agaaatactt agaggaagaa atttcgactt
     6481 ctcttcttca acattcaaat gcacagtttt ctatttcatg gatgaatcag caatcgatca
     6541 ttgaacaaag acagttgctt gctgaaaaag aactggttaa cttagatcag cagatgaaag
     6601 aagatgaaac tttattagca gattcgttac aaaaacgtct ggcatatgaa tatccttatc
     6661 aagcttcttc acaaaccaca agttatcaat ctgtatctga aatcaaacga ttattcgaag
     6721 atccagatga tacacaagaa tcacgtttga ctctggaaag cagtcagaac aaagcagcat
     6781 cccgtcaatt tcgttatacg caagaacaac tagcagaacc aaaatttttg caaaaagatc
     6841 ggcaagtgtc agcagctgct gttggaactg ctacgcatgc tttgttacaa ttactgccat
     6901 tagagatgcc aactacagag agtatccatc aaaaattaca ggaattggta aggaaacggt
     6961 tagtagacga aaaagtcgcg aaaaaagtag atgtttcttc tattatatgg ttctttcaaa
     7021 cagaacttgg tcaacagctg attgccaaca aagaaaacgt aaaacgagaa caaccttttt
     7081 ccatgctgct tcctgctgac gaagtatttc aagactatcc aaatcaagaa gatgaactat
     7141 taatccacgg aatcgtcgat ggatacctag aagaaaaaga tcatctcaat atctatgatt
     7201 ttaaaacaga ttttatttta catccggatg atccagcaga aatagatgca atcgtccaaa
     7261 aatatcaagg acagctaaga ctttaccaac aagcaatgtc agaagctttg aataagccag
     7321 tagaaaatgt tttcctaatt ttgcttcgag tgaaacaaat tataaacatt aacaaataat
     7381 tataattgag caaacttgac ttatgttttg taaggattat gctatactct gggaagaggt
     7441 gagtatgatg gagctaacac agacagaatt cgttttatgc cctaaatttg aaaaaacatt
     7501 ctctatttta ggaaaaaaat ggaacggatt gattatcgat gtactactcg aaaacggtcc
     7561 gcaacgcttt gttgagcttg cggcaaaaat cccgatggtc agtgatcgag tattagtcga
     7621 acgcttgaag gaattagaaa aagaaggaat agtcactcga aactttgcct gtagcgaaaa
     7681 gagccgttca gaatatctct tgactgaaaa aggagcagac ttgcaaaaag cgatgcgtca
     7741 aatacaagtt tggagcgaaa aatggatcac agaggaagaa tgcggttgat tttgtaaaaa
     7801 aaatcttgta aactatagtc catacgaata agtaaagaac gatgacgaaa aagagtagtt
     7861 tggctaaacc agttcaggga gatctgtcgc agactgaaag cagatcctgt gtttgaaaaa
     7921 tgaagttcac tttccgagtt cgtctgataa aatcagaccg gcaattgtcg ttaacgaagt
     7981 aagagtgcgg tgtaagtcat ttatccgcaa attaggatgg taacgcgaag acctcgttcc
     8041 ttaacaggaa tgaggtcttt ttgttttgta tttggaattt aaaggaggaa caagcacatg
     8101 tctctacaag cacaattaga gagcttgaaa gaagcaacgc tcgtcaaaat cacagcggca
     8161 caagatttaa atgaattaaa tcaaatacgt gttgaaacgt taggaaaaaa aggaccgatc
     8221 acagaagtat tacgtgggat gaaagatcta agtccagaag agcgaccaaa agtcggaagt
     8281 ttcgcaaatg agatccgaga tcttttgaca gaagagatcg agcgaaaaaa acaagtatta
     8341 gaagaagaag cgttaaatgc agctttggca aaagaaacaa tagacgtgac actacctggt
     8401 agaaaagtaa aacatggtac acgtcatatc gtgacacaaa tcatggaaga aattgaagat
     8461 atttttgtgg gtatgggcta tcagatcgta gaaggatatg aagtagaatc tgatcattac
     8521 aatttcgaac ggatgaatct gccaaaagat catccagcaa gggacatgca agatactttc
     8581 tatatttcag acgagatatt gattcggaca catacttcac cagtccaagc acggacaatg
     8641 gaaaaacatg atttctctaa gggtgctcta cgaatgatct cgccagggaa agtcttccgc
     8701 agagatacag atgatgcgac ccacagtcat cagttccatc aaattgaagg gctagttgtt
     8761 gataaaaaca tcacaatggg cgaccttaaa ggaacattag aagtcgtaat gaaaaaaatg
     8821 tttggggaag atcgtgaaat tcgtttgcgt ccaagttatt tcccatttac ggaaccatca
     8881 gtagaagtag atgtcagctg tttcaaatgt ggtggtgccg gttgtaacgt atgtaaatac
     8941 actggatgga tcgaaatttt aggagctggc atggtacacc cgaatgtgtt gaagatgtca
     9001 ggaatcgatc cagaagaata ttcaggcttt gctttcggcc ttggaccaga ccgcgtagct
     9061 atgttgcggt atggagtgaa tgatatccgt aatttctatc aaaatgactt acgtttctta
     9121 agtcagttca agggggagta agaaaatgtt agtttcatat aaatggctca atcgttatgt
     9181 agatttatcg aatgtaacac caaaagaatt agcggataag atgtctgtga caggaatcga
     9241 agtagaggga gtaacggttc cagaagaagg cttgaaaaaa atcgttgtcg gtgaagtcaa
     9301 ggaatgcgta ccacatccag attctgatca cctttctatt tgtcaagtcg atattggcga
     9361 agacgaactg tctcaaatcg tttgtggagc gcctaatgtg aaggttggaa tcaaagtgat
     9421 tgttgcactt cctaactcac gtatcgctgg taatgtaaaa atcaaaaaag gaaaaatgcg
     9481 tggccaagta tcaaatggca tgatttgctc attacaagaa attggctatt cagatagtgt
     9541 tataccgaaa gaatacgctg aaggtatcta ttatatgcca caagaagctg taaatggcga
     9601 tccagttttt ccttatttgg atatggatga ttcgattatt gaattatcta tcacaccgaa
     9661 tcgtgcggat gcattgtcaa tgcgtggcgt agcttatgaa gttggggcaa tttatcgtca
     9721 gactcctaca ttcgatgacg aaaagctggt agaatcctca cgtcctgcta gcgataaaat
     9781 tcaagtggaa gttgaagata aagaagcagt tcctgcttat caaatcagaa tcattgaagg
     9841 agtaagaatc cagccaagtc ctcaatggct gcaaaatctt ttgatgaacg aaggcattcg
     9901 tccaatcaac aatgtagtag atgtgacgaa ctatattttg cttttattcg gacaaccact
     9961 acatgcattt gactacgaca aattgaaatc aaaagagatc tttgtccgcc gtgcaaaaga
    10021 aaatgaaaca ttagtaacac tagatggaga atctcgggaa ttgacaactg acaatctcgt
    10081 cattactaac ggcaaagaac cagttgcttt agctggtgtc atgggagggt tagactcaga
    10141 gatcacgaat gaaacaacaa cagttgcact agaatctgct ttatttgatc caatttcgat
    10201 tcgtcgtaca gcaaaacaat ttaatttgcg tagtgaatcc tctgcacgtt ttgaaaaagg
    10261 aatcaaccat gcgactgttg gagaagccag tgaggttgct gcagcaatga tcgtttctct
    10321 tgcgggtggg gaagttctac aaggcgcggt caaaggctca gagatcaaag cagaagacgt
    10381 atcagtgaag atttcacttg aacgcatcaa tcaatattta ggaacatctc ttgtcgttaa
    10441 agaagtaaat gagatttttg aagcacttgg atttagctat gaagaaaagg agtatgttta
    10501 ttccgtagta gtaccaccaa gacgctggga tatccaaatc gaagcagata ttatcgaaga
    10561 agttgctcgt atttatggat acgaccagtt gccatctaca cttccaagtg gagaaacggt
    10621 tgctggaagc ttgtcaaaag aacaagaaac tactcgtaaa attcgtacaa tctttgaagg
    10681 aagcggattg agcgaagcaa tcagttatgc attgacaaca gaagaaaaat caagacagtt
    10741 tgttataaat gaaagtaata ttacacgttt ggactggcca atgagcgaag aacgttctgt
    10801 attacgaatg aatttgatca gcggactatt agatgatatc agttataaca ctgcccgaaa
    10861 aaatacagag attgctttat atgaaattgg tcgggtgttc ttccaagaaa atgatccacg
    10921 tacacatctg ccgcaagaag taaaacatgc agcaatcgct atttctggta tttgggaaga
    10981 aaaagattgg cagacgaaag ctcaaaatgt agactttttc accatcaagg gtcttttaga
    11041 aaacttattt gaacaactag gcatcactga agatgtccat tatcaaacag tgacagaaat
    11101 gaaggaaatg catcctggaa gaactgctgc gatttacctt ggcgaaaaat ttattggttt
    11161 tgtcggtcaa gtgcatccaa cgattacaaa aagttatgat attccagaaa catatgttgc
    11221 tgagtttgat ttatctgcta tagtggaagc tgctcaaaaa gggatcgttt tcgaagcagt
    11281 gacaaaattc ccagcagtca gccgggacat cgctttgctt gtaaaagaaa ctgtgacaaa
    11341 tcaggaatta acagaagtta tccgttctgc tgccggaaga tttttaacag atatccaatt
    11401 atttgatgtc tatcaaggag aaaacattga aaaaggacat aaatccatgg cgtatagcct
    11461 gacattcgtc aacccagaag caacattgac agatgaagaa atcaataaag caatggaaaa
    11521 agtcgaaaaa gcgttggtag aaaacctaga ggcaagtatt cgctaagaag gaaaatatag
    11581 taagtaacga tcaagtagct tttgaagacc atacatttga aaaaaagatg tgacacaact
    11641 tcgtcacatc tctttttttg attttaaaaa aggaatagca cagagacgag ataaaaaaac
    11701 aggcctttac agcctgcttt tttagagtaa atgaattaat ggaagtaatt gattcccatt
    11761 gctgatttta cttcagaaag tgtttgtgca gctactttag cagcttcttc actaccttta
    11821 cgtaagattt ccataacagc tgaaggatct ttttccaatt cttctcggcg cgcacggatc
    11881 ggtgcaaatt cagcttccaa tacatcaatg aggtatcgtt tgatctttac atcaccaagt
    11941 ccgccgcggc ggtaatgagc tttcatttct tcaattgctt ctttatctgt tccgaataca
    12001 tccaagtagg tgaagaccat attcccttct acttgtccag gatcttgtac atggatgtgg
    12061 tttggatcag tatacatact catgactttt ttctgtaaga catcggcagg atcggagata
    12121 taaataccgt ttcctaacga tttactcatt ttgccatttc catcgattcc aggtaatcta
    12181 cccattcctt ttggtgggaa tacgcctttt ggttccacca agacttcacc atatgtatga
    12241 ttgaagcttt gtacgatttc ctgtgtctgc tctagcattg gtttttgatc ctcaccaaca
    12301 ggtacaagat tcgctttgaa tgcagtgata tctgctgctt gagaaacagg atagataaag
    12361 aatcctgtag gcacgctttc accgaatttt ttttgttcga tttctgtttt gacagtcgga
    12421 ttgcgccgaa cgcgtcctac gctgactaag ttcaaataat acatggttaa ttcagctaat
    12481 tccggaattt gtgattggat aaataaagta gattttgcag gatctaatcc cacggctaaa
    12541 tagtctaaag caacttgcaa gacatttgat gaaaccttct caggattttt agcattatct
    12601 gtcagtgctt gcatgtcagc aatcatgaca aacagttggt tgtcttcatc tgcctgcata
    12661 gccactcggg tttttagaga accgacgtaa tgccctaaat gcagcttccc agttggtcga
    12721 tctcccgtta aaataatatt tttcaaaaaa gacacctctt tacgatttgt aagtctgagc
    12781 acttaatatc ttaccgtttt tcctgaaaag aaacaacagt tcctaaggaa aaaacaagga
    12841 aagaggataa aataactaat agattagaaa tggaagaaat atttatggga gaataaacgt
    12901 tctaagaaac tttacaaatt actaaactat cagatataat gtattttatt atacatcttt
    12961 aaaagaaatc ttactaaata ataatgggag tgtaattcta tggcaatgat taaatttgat
    13021 cacgtggata aatattatgg caagttccac gcattaaaaa atatcaatct cgaattcgag
    13081 aaaggagaag ttgtcgttgt cattggtcct tctggttcag gaaagagcac gatgcttcgc
    13141 tgtatcaatg gattagagac gatctcttca ggtaagctgc tgattaatga tactgacttg
    13201 catgataaaa agacaaaact gacagaagtc cgaaaaaaca tcggcatggt tttccaacat
    13261 ttcaatttgt acccaaataa aactgttctg gaaaacatca aattagcacc aatcaaagtg
    13321 ttgaagcaag accaggcaac tgcagtcaaa aacgcggaga aatttttgaa aactgtcaat
    13381 atgttagata agaaagattc ttatccttcc atgttatccg ggggacaaca gcaacgggtg
    13441 gcaatcgcca gaggtttagc gatgaatcct gagatgctgc tgtttgatga acctacttct
    13501 gctttggatc cggaaatgat tggtgatgta ttagacgtta tgaaaaaatt ggctcatgac
    13561 ggtatgtcaa tgattgtcgt gacacatgaa atgggctttg ccaaagaagt ggcggatcgt
    13621 gttatcttca tggctgatgg agaagtattg gaagacagca gaaatgtgag agactttttc
    13681 gaaaatccaa atgaagtccg agcacagcaa tttatcagta aagttatcaa tcattgatag
    13741 gaggaaaaag atgcgcaggt taaaaaaaat acgcctgatt ttccttttag gcattgcttt
    13801 tcttgcacta gccggatgta aaggaaatgg gttggcaaat gaagatattt tagagcgaag
    13861 caaatctacc aatgaaatca tctggggtgt caagtatgat actcgattat ttggaatgat
    13921 ggatattgag agcagaactg tacaaggatt tgatgtagat attgcaaaag caatcactaa
    13981 aaagatcctt ggtgacaatg gaaaaacaga atttgttgaa gtcacttcta aaactcggat
    14041 tccattattg aaaaacggaa atatcgatgc aattattgca acgatgacga tcacagatga
    14101 acgaaaaaaa caggttgact tttcagatgt ctatttcgat gctggccaag ctttgttggt
    14161 aaagaaagga agccaaatca aaagtgtaga tgatttgaat gcttctacaa cggttctagc
    14221 agtcaaaggt tctacatcgg ctgcgaatat ccgacagcat gcaccagatg caaaaatttt
    14281 agaattagaa aactatgcag aagcatttac tgctttacaa tctggtcagg gagacgcgat
    14341 gactacggat aatgcgattt tgttagggat tgcagatgaa aatccagagt atgagttagt
    14401 tggtggaacg tttactaatg agccttacgg gattgcgatc aacaaagggc aagaaaactt
    14461 cttgaaagct gttaaccaag cgctagagga aatgcatgca gatggcacat acgataaaat
    14521 ctatcaaaaa tggtttccaa atgaaactga aggaaaagta gagtaagggg gagaggcaat
    14581 gagcgaattg atacaaactt atggaccaat ctttgtcgac gggttcaaga tcacattgtt
    14641 ttcgagtgtc ttagcacttg tgtttagttt gattatcgga acgttaatgg caatcttcca
    14701 attatcaaag aataaagtag tgagcgcctt agcaaaggca tatgtagaat tttttagaaa
    14761 cattccatta ttaatcatcg tgatgttttt ttatgttgtc gtaccgattt attggtttaa
    14821 tctagatgga ttccaagcag gtacgattgg attaacgatc tatacatctg cttttattgc
    14881 tgaaacagta cgttcaggga ttcaaaccgt accaaaaggt caaacagaag caggactatc
    14941 ttctggtttt acctattcag aaatcatgcg ttatattgtt ttaccgcaag cattcaaaat
    15001 cgttatcccg ccattaggca accaatttat caacctcgta aaaaactcat ctattttagc
    15061 aatcgtagct ggtttagatt tgatgtatca aggagacttg atcgctagtg aaacattcaa
    15121 taccttcgat acgtatatca tcgttgggtt attttactta gttatcacgc taccgttatc
    15181 ttacttaatg gtttatcttg agaaaaaatg ggcggttcgt tcatagaagg aggggaaata
    15241 catggatttt agcggcgctt tttcatggat gaatatccgt tttttattgg aaggattaaa
    15301 ggttactgtt gaagtttcat tgttttcgat catttttagt ttcttgatcg gtggtctgac
    15361 aggtgtgatt cgttttgcga atatcccttt cttttccaaa atccttggtg tagtgattga
    15421 tattatacgg aatcttccat tattattgat cattttcttc acttactttg ccttacctca
    15481 aatcggcatt cagatgaaca tcttctggtc cgctgtcgca gcattaacga tttttgagtc
    15541 agctatgttg tcggaaattt tccgagctgg actcaatgct gttccaaaag gacagatgga
    15601 agcaggatta tcaacgggac tgacttatgt agaatcgatg cgaacgatcg tgttaccgca
    15661 agcatttaaa tcaatgatcc cggcaattgt gagccaattg atttcactga tcaaagatac
    15721 ttcattagca gtaatcattt ctttacctga actttctcat caggcacgta ttatttatgg
    15781 acagaacacc aattatgtgc ttccgatgtt catcatcatg acacttatgt acttcgtggt
    15841 atgttacgcg ctttctctgc tttccggtta cttggaaaag agacggtaca gttattaata
    15901 gatgattctt ttagcggttg tggcaaacta aagtcacaac cgcttttcct ctttccattc
    15961 gaaaaaaaga gatttcgatt gttttaagca agtaagagca cttgaaaatt cacgctaaaa
    16021 agtacacaat agggaggata gaatttattg aaaaaaagga tgatacgatt gacagataat
    16081 cgccctatcg gatttattga ttcaggtgtc ggcggcttga ctgtagtaaa agaagccctg
    16141 aaacaattac cgaatgaaaa tattttattt gtaggagaca cagcacgctg cccatatggc
    16201 cctagacccg cggaacaggt aatccagtat acttgggaaa tgacggatta tctggtggag
    16261 caaggaatca agatgctggt gatcgcctgc aataccgcaa ctgcggtggc tttagaagaa
    16321 atcaaagctg ctctttctat tccagtcatc ggtgtgatcc ttcccggtac tagagcggca
    16381 gtaaaaaaaa cacaaaataa acaagttggc attatcggta cgattggtac ggtaaaaagt
    16441 caagcttatg aaaaagcact gaaagagaaa gtaccagaat tgactgtgac aagtcttgct
    16501 tgtcccaaat ttgtttcagt tgtcgaaagt aatgaatacc attcatcggt ggcgaaaaaa
    16561 attgtggcag aaacattagc tcctttaacc actaaaaaaa tcgatacatt gattttggga
    16621 tgcacccatt atccattatt acgccccatc attcaaaatg taatgggaga aaatgttcaa
    16681 ctgatcgatt ctggagcaga aacagtaggt gaagtatcta tgctgttaga ttatttcaat
    16741 ttgagcaatt caccgcaaaa tggtcggaca ttatgccagt tttatacaac tggctctgcc
    16801 aaacttttcg aggaaatagc tgaagactgg cttggaatcg gacacttaaa tgtagaacat
    16861 atcgaattgg gaggaaaata aggatgcgtc atgacgggag aaaagcccaa gagcttcgca
    16921 aactggagct tcaaacaaat gttttcaaat atccagaagg atcggtagtg atccgttttg
    16981 gtgatacaac agttatttgt tctgcaacaa tagaaaattc tgttccccct tttttgagag
    17041 aaacaggaac aggatgggtg acagcagaat atagtatgct gcctcgagca acgagtacga
    17101 gaaatcgtag agaaagcagc aaagggaaac tttctggtag aacaatggaa atccagcgtc
    17161 ttattggtcg ttccttgcgt gcagtagtag acttggaaaa attaggagaa cgtagtatca
    17221 tcgtagactg cgatgtgatc caagcggatg gcggtactag aacagcaagt atcactgggg
    17281 catttgtggc attgaaatta gccattgaaa aacttttaag agaaaaagaa ttgtcagaag
    17341 atccaataaa agaacatctt gctgcagtta gcgtaggaat cttaccagat gggacttgcg
    17401 tcacagattt ggattaccaa gaagattccg cagcacttgt agacatgaat cttgtaatga
    17461 cagaatctgg aaaattcgtg gagattcaag gaactggaga agaagcaacg tttgatggtg
    17521 aacagttgaa tgaaatgctc ttttttggaa aaaacgcgat tgaagatttg atcaaagagc
    17581 aaaaacatgc tttgctaaca gagtttgctc aaaatgatga gcggatagaa gaaacaaaaa
    17641 caatcatcat tgcgacaaga aatccaggaa aagcagaaga gtttcgaaat atgttcaaag
    17701 aagcgggata tcatgtaaaa acattacttg actatcctga attgccagat gtggaagaaa
    17761 ctggcagcac atttgaagaa aatgcccgat taaaagcaga aatgattgca caattgcttg
    17821 atcagccagt cttagcagat gattcaggtt tgaaagtgga tgctttaggc ggtatgccag
    17881 gaatctattc tgcacgtttt gcaggggaac aaaaaagtga tgcggggaat aacgccaaat
    17941 tattatatga attgacagat gtgccggatg aaaaaagaac agcacaattc cattgtacgt
    18001 tagtattcgc ggcacccaaa aaagacagcc ttgtagtaga agcggaatgg cccggtcgag
    18061 tagcccgtat accatctggc gaaaatggct ttgggtatga tcctttattt atcccagaag
    18121 gtaagaaaca aacagcagca gaattatcga gcgaagaaaa aaacaagatc agtcatcggg
    18181 ctcaagcaat gaaaaaattg agtgccgagt ggaaacaatg gctggaagga gagagataga
    18241 tgcggtattt agtagtaagt gataatcatg gagacagaga tatcctcgtt gacttagtag
    18301 atcgttatcg ttctgaagtg gatatctttt tccattgtgg tgattcggaa ttagaagctt
    18361 ctgacccatt atgggataca ttccaagtgg ttcaaggtaa ttgtgattat ggaccaggat
    18421 tcgaacagaa aaaagtgatc caaactggtc aggatacagt attcatgact cacggtcatt
    18481 tatcgaatgt ccgatttggt ttgacacagt tagcaatcga agctcgagct gcaactgccg
    18541 atatggtgtt ttttggtcat acccatcaaa taggctgtga gatggatcag aaaatcctgt
    18601 ttttgaatcc tggtagcatt tctcagccta gaggcccgat ccaaatccca tcatatgctc
    18661 ttatcgaaag tttagatgac caaatttctg ttcaatacta taaccgtgcc catcaggcag
    18721 ttgaagagtt ggccttttct ttttcgagag agcgctgaat aaaaaaactc taatttttaa
    18781 taaaaataag ctttatttta tgaattttgc atccggtttc ggtaaaatag tagtgttgaa
    18841 tgaaatagaa atggaggcca tcagatgatt ggacctatag taagagaaat gctgctggaa
    18901 aatcaggaaa cgtttttgat tcctgcagaa aatgtggcaa atgttatgta tcatcatcca
    18961 ttgtcccatg gtttacttgt gttatcgaag gtcggttatt ccaaaattcc tgtattagga
    19021 aaagatgatc actttgtagg actagtaagt ttatcagatg tggtcaataa aatgattgac
    19081 ttgcaaacaa tcagcatgga cccgttagaa ggactaacgg ttgctgatgt gacagaaaca
    19141 gatgttccga caattgatga aaattgggaa ctagaagaag tactccattt gttagtcgat
    19201 tcgccttttt taccggttgt cacagaagat cggattttca aaggaatcat tacaagaaaa
    19261 gagctgctta aagccgtaaa cttcatggtg catgaattag aacgtcgtaa tatattgtct
    19321 ccgaaaatcg aagaattaaa agaacaaatc gatatcgtga gctaaagaac gagaaactaa
    19381 aagataatca aaaaataagt agaaaaaaca gcgtaccagt tttgacttta tcttgaaaaa
    19441 gaggctggga caaaagcgtt taactccaag aaataagaag gaattcacga aaattgcttt
    19501 tcaaattttt gtgaatttca gcttatttcc gaaggagttg cttctgcttc cgccgtttat
    19561 acgtgtttag agcgtgggac aaaagtgttc tttacttttg tcccacgctc tttttagtga
    19621 gattcgttgc atcgtttgtc caaatt
//
LOCUS       DAZKKW010000050        19408 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.050, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000050 DAZKKW010000000
VERSION     DAZKKW010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19408
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.050"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            104..541
                     /locus_tag="ACG8PO_001712"
     CDS             104..541
                     /locus_tag="ACG8PO_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0207024.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWMFYLVKK"
     gene            636..1520
                     /locus_tag="ACG8PO_001713"
     CDS             636..1520
                     /locus_tag="ACG8PO_001713"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFQ0207025.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            1744..4911
                     /locus_tag="ACG8PO_001714"
     CDS             1744..4911
                     /locus_tag="ACG8PO_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HFQ0207026.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSKYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEITWTLADAPTE"
     gene            4979..5476
                     /locus_tag="ACG8PO_001715"
     CDS             4979..5476
                     /locus_tag="ACG8PO_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 domain-containing protein"
                     /protein_id="HFQ0207027.1"
                     /translation="MILLLGFILWQTPVHASEFNFAVDFELPANQIDKNKTYINVRME
                     PGKTQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESKSKFDETLSYDMKNLAK
                     TVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKENVSNKSTVGVVNQFSQV
                     KAILI"
     gene            5495..5959
                     /locus_tag="ACG8PO_001716"
                     /pseudo
     CDS             5495..5959
                     /locus_tag="ACG8PO_001716"
                     /inference="COORDINATES: protein motif:HMM:NF023225.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3324 domain-containing protein"
     gene            5956..6714
                     /locus_tag="ACG8PO_001717"
     CDS             5956..6714
                     /locus_tag="ACG8PO_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFQ0207028.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            complement(6800..7540)
                     /locus_tag="ACG8PO_001718"
     CDS             complement(6800..7540)
                     /locus_tag="ACG8PO_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFQ0207029.1"
                     /translation="MKKYFIIEDNQFHSEWIIRELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            7601..8854
                     /locus_tag="ACG8PO_001719"
     CDS             7601..8854
                     /locus_tag="ACG8PO_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFQ0207030.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLIGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            9176..9403
                     /locus_tag="ACG8PO_001720"
     CDS             9176..9403
                     /locus_tag="ACG8PO_001720"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFQ0207031.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            9526..9852
                     /locus_tag="ACG8PO_001721"
     CDS             9526..9852
                     /locus_tag="ACG8PO_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HFQ0207032.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            9920..10633
                     /locus_tag="ACG8PO_001722"
     CDS             9920..10633
                     /locus_tag="ACG8PO_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HFQ0207033.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            10943..11794
                     /gene="licT"
                     /locus_tag="ACG8PO_001723"
     CDS             10943..11794
                     /gene="licT"
                     /locus_tag="ACG8PO_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
                     /protein_id="HFQ0207034.1"
                     /translation="MKIDKILNNNVVISKNGFGEEVVCMGKGLAFQKKIGDEISPEAV
                     QKEFVLRDSFAKNQFQQLMADIPAEEIEWVKQVVELAEDKLKVEFSPNIYLTLTDHLH
                     YAITRAKENIQLPNPLLFETKKFYPKEYQAAKEALLFVKQKTGVELSEDEAGFIAFHF
                     VNSQQGNEDMQVTMTATTMVRDILSIISKFFGIVFDEESLNYQRIITHLQFFAQRYLK
                     GEGSDEQDEFLYALVQGKYPKAFHCVERINEYLRQTQKQEMGIAEQIYLTIHIQRVVS
                     EKKSIQS"
     gene            11915..13780
                     /locus_tag="ACG8PO_001724"
     CDS             11915..13780
                     /locus_tag="ACG8PO_001724"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFQ0207035.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            13794..15236
                     /locus_tag="ACG8PO_001725"
     CDS             13794..15236
                     /locus_tag="ACG8PO_001725"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFQ0207036.1"
                     /translation="MQSRFSEKFLWGGATAANQLEGAYDRDGKGLSVADAMPGGKQRF
                     AIIGSEEFDWTIDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPN
                     GDEGTPNEAGLEFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYE
                     RFAQTVLERYSSKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVA
                     SSKAVKIGHELRSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGE
                     YPAYTARTHKKYGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSE
                     GNLLGGVKNPFLEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDG
                     TIIDDYRIDYLRRHIEAMANAVADGVDLMGYTPWGCMDLVSASTGEMSKRYGFIYVDL
                     DDEGNGTLNRSKKKSFDWYKEVIASNGVNL"
     gene            15344..16045
                     /locus_tag="ACG8PO_001726"
     CDS             15344..16045
                     /locus_tag="ACG8PO_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0207037.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQEILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            16184..16696
                     /locus_tag="ACG8PO_001727"
                     /pseudo
     CDS             16184..16696
                     /locus_tag="ACG8PO_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014566402.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            16707..17213
                     /locus_tag="ACG8PO_001728"
     CDS             16707..17213
                     /locus_tag="ACG8PO_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lyxose/D-mannose family sugar isomerase"
                     /protein_id="HFQ0207038.1"
                     /translation="MDYKKRVQQMFLDSGVVLKETELERIEYTDFGLGAIETEGLNLF
                     VYENNERYCGKELVLLPYQTCPEHRHPPRDENEGKKETFRCRKGLVYLYVEGKETKKL
                     SCIIPKGQERYYTAKKEIILHAGDQYTIEQNIKHWFQAGATGAVITEFSSSSDDPSDS
                     FTNPAIYR"
     gene            17624..18199
                     /locus_tag="ACG8PO_001729"
     CDS             17624..18199
                     /locus_tag="ACG8PO_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HFQ0207039.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            18199..18639
                     /locus_tag="ACG8PO_001730"
     CDS             18199..18639
                     /locus_tag="ACG8PO_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HFQ0207040.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVLAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFEKLPVETYVGEYL"
     gene            18655..19356
                     /locus_tag="ACG8PO_001731"
     CDS             18655..19356
                     /locus_tag="ACG8PO_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFQ0207041.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDTFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
BASE COUNT         6497 a         3223 c         4171 g         5517 t
ORIGIN      
        1 ttttaaaaat tttttttact tagaatccag cgaccctaga tatttttctg tataatctta
       61 aataaagaaa acaatatcaa aaaatgcacg tagaagtaga taaatggagt ggttttgcaa
      121 atttctttta tttctagaag ttattcacgt atacggattt gtttttctca caaataaaaa
      181 ggcaggaagt acaatggatc atttgtatat aaaatatatt catcaatttt ccgttttggt
      241 gttaggcaca ctaattatag cggggattgt cgcatttatt attcaaggcc gtgacatatt
      301 aacagtacat caagagtttt tcagttatgc atttggtatt cctctgatcg ctataaggtc
      361 tgataacaaa aagtggaaga aacgctatta cattctcttg ctatttttgt gtttttatct
      421 gccacctaag atttttactt tattaggtat acaatgccta tcattttctt ttatttccta
      481 tatcattgtt ttaattttcc tcagtttcat agtatggatg ttttatcttg tcaaaaaata
      541 aatgaatgtt tcatgaacag tacttcatca cagcctaatt tttttcttta tgctatcgtt
      601 atattaaaga aatcaatcag aagggaaaga aagtgatgaa aaaagtttta ttagtggtaa
      661 accccagttc aggcggtgaa caagcaaagg aatttgagca attagcgata gcaaaattgg
      721 aatcggtgtt tgatgaagtg gtcgtcttgc atacaaaaaa agcaggggat gcaaaaaact
      781 ttactcgcga agcggctacg gaagggtatc atagtgtgtt tgtcatgggc ggagatggaa
      841 cagtcaacga aggaatcagt ggaatcgctg aacaagaaca tcggccaaat ttcggatttt
      901 tcccattggg tacggtgaat gacttggctc gagcattagg gataccgcta gaacctgagg
      961 aagcgatcaa tcatttttcg attgaatcag tcaagccatt ggatattgga aaaataaatg
     1021 acgactattt tatgaatgtc gtcgcaattg gctctatccc tgaatcaatc aatgatgtag
     1081 atgcggagaa gaagacgaaa tttggaaaac tagcttattt catgtcagga atcaaacagt
     1141 tagcttccac acaatcctac tcgtttcacg tagaagttga tggaaagaag gaagaaattg
     1201 agagcagtac cttgctgata ggactaacta actcagtcgg cggttttgaa acattacttc
     1261 caaatgcaaa agtggatgat ggtaagctcc atttggttta tttaaaagat tcttctttga
     1321 tcgatacgct taaaacattg ccagacctat taaaaggagt agatgaatct acgaaaaatc
     1381 tagcctacct tacttgtgag caattagttg tgtcattagc tgatgaggaa gaattgacga
     1441 cgaatgttga tggagatgaa ggggataaat tacctgtgac aatcaaagta ttgccctcac
     1501 atttacaggt ttattgttaa aatggaggtt gtgaccgagg agttggcggg agaacaccgc
     1561 ttgttcggga taagggactg tgacaagact tttgtcacag cctttttttt gtacaaaaaa
     1621 gaaatttaca cctaatagaa aaatttttcc ataaaaatac atattttaaa tatgaaagta
     1681 caattggaaa tagattaaaa cgataaaata aaattgtcaa aacgtcagag aggatggtgg
     1741 agaatgaatc aatgtgtaaa aaagctgatt gcgtcgattt tgtttgtctt ccctctcttg
     1801 atggtgacat ccgtttttgc cgatgtagag aaaacaaacg aatcaacgca atcagagaat
     1861 caaagttttg acaagactac tttttcagaa gaatcgatta cttctgaaac tacagaatct
     1921 caaagtgagg cttctactaa taaaaggcaa gaagaaaata agacaagtga gacctcagaa
     1981 gtagctgaac aaacgtctga tagcagtgaa gcggcactgg caagagctgt ttcagcggat
     2041 gggtacagcg aagctgccac aacagaagaa ctggcgcagc ttctcgcaga cgaatcagta
     2101 actaagatcc gactgattca gcctttgact ttagacaggg aattagagat caaacgagat
     2161 atcgttattg attttggtgg attcgctcac aatttcggca cacaccatat ttatatcaat
     2221 gagatacctc agtcgatcga gttccagaat tttaaaggaa cagctgcaca tccaggtgga
     2281 ttgacaccaa atatcgatgg aaatgccatt atcattgctt atatgaccga tttttgggga
     2341 agcaaatatc attttacggg agaaataaaa tttaccggga cacttgattt gtatgatggg
     2401 tccaaattag gactgattta cgctcctcgt gcaactgtca cattagatgg tgtttccggt
     2461 gtgttggatg tccaaccagt gaaggaagga ctgaatgccg ctccgggtaa agcctatttt
     2521 tgtagaagct atcaattgaa tgtgatcaat ggtagtcaac tttatggacc gtatctaggg
     2581 aaattctacg gttttctaga ttcaggagat gagactggta gtggaaaaac agcgccagga
     2641 atccatatct taagcggttc aaaggtctct cttgattatg atcgggcaga tggcgttagc
     2701 gatgaaggag aggcagtcga tacgctacct ggtaatgtca tgtttgaagt cagtggttcg
     2761 ggaagcgaat tttcggtaaa tacaaagatc aatattacca atgacaacaa tcgcggaatc
     2821 atccaaatga gaggttcagg atcgaaagtc actgtatcga atggtgggaa aatcaccatc
     2881 aatacagcaa ctacgggcgg atttcgcttg caaggagatt ctagtcaaat ccatgtatca
     2941 agtggtggac aaatctcggt caaaatggct ggagatgacg atcaacccgg gaacaatggg
     3001 atgcgttttg taggaaaagg cctttctttg atcgtagaag gagccggatc agttatcgac
     3061 atcgataagc agagtgggcg ttccagcgca gttcgttttg agaatggtac gcaaaagctg
     3121 acagtaacca atggaggctc attgagggta agaaatacag gtgatggcaa atcttacgtg
     3181 aacagactca atcaaggaaa tcaagcgatc caattttatg acgcttctgg ttttctagaa
     3241 agtccaggtt cagctgattt tacagtcact ggagaaaaat caaatatcga tatacgcgca
     3301 gattccggag caactgtaga tacaactgga gatattgatt taaattttac ggcaggtgaa
     3361 aaaacttatt tagtcatggt cgggaaaaca ggggaagata agaccggcat catcagtggg
     3421 gatgtgttga atgtggactt agaatctcct acctattttg atttccaaaa caaacggatt
     3481 ggaagcgaga ccaatggcat aggcggctgg gtattccaag gaaatggaga ttcttcttta
     3541 aagatcgccc attcagaaat cgctttatgg cgaaaaacag gcgcaacatt cgataacttt
     3601 gaaaaagacc cggattattt ttctgatatg acagacttat cgatcaatgg gaccgactta
     3661 ggcaatttcg tatcaagccc tagtgcaggt ttaacaaatg aatttgctca gacaggtgcc
     3721 ggaatgaaaa attataatcg aatcagtgcc aataatcagc tcccgatgat tgaatcctta
     3781 agagtgccaa cgaatgcaga taaaaaaatc tatgggcatg tcgttgttcc cgaaggtgtc
     3841 gaggcaaagc caagagatgc ttggacaggc gaagtagaag tgactttacg tattacttac
     3901 gcagatacga cgaaatcatc tgtagaaatt actgctctca cgcaaggaca aacagatgaa
     3961 aatgaagggg caggatataa cccatgggga gaaggcgaac aaggcggctt gttcgaagtt
     4021 caagtaccca atgatgagag attaaaaacg gaggatcaag tgcaagttgt tgctgctaaa
     4081 aaagtaagag gtggccaaga agtgacagaa ctggactctg ccaaaacgac agtcgatgtg
     4141 attcctcctg aacctgctaa tttaagtacc acattgatca atgcagcttc tcgcacgatc
     4201 acaggtacag gtgaagcagg agccttggtt tcattgaaaa aaaatcaagc tgttttgtca
     4261 gaaacaacta ttgatgatac aggggaattt gttctttcta tcccagaaaa tcaacttttt
     4321 gcaggagatg tattagaaat tgtattaaac gaccaagcag gagaagctac tgctaagggt
     4381 gtgcagaaca agccaagtac caacgatgaa attgggaatc acaatccaag tgatacgcca
     4441 gttgtttatc atgatgcccc gccttttgca cctgctacga agatcgtagt agaaggtggt
     4501 ctctcatttc ttgccccttc caaactcgat tttggtcaga tcaaagcaac tggtttgaca
     4561 caagaagcat ttggccaaat ggatatacag gaaaaattat ggatttatga tcagcgagaa
     4621 acgaaaaatc cgtggatctt aacgataaaa ttatctcagc ctttcacaaa tgatgcagga
     4681 ttttctatgc aggatgtcct atatttcaag aaaggagagg agtatcaatt agtgaatgaa
     4741 gaagaactgg aaattgtcag aggacaaaat tcaacgaatg tgcaagcaga ttattcagaa
     4801 tatttaaata acggtcgggc gtttaaactt gtcttaacca aagaaaatca gatagctggt
     4861 aattactcag cagagataac atggacatta gcagatgcgc cgacagaata aagatcaagg
     4921 aggatgattg atgaatcgaa tatggaataa aagtcatcga aacaactgct ctttacttat
     4981 gatccttctc cttggattca tcctttggca gacacctgtc catgcctccg aattcaattt
     5041 tgcggtagat tttgaattac cggctaatca gattgataag aacaaaacat atataaatgt
     5101 aaggatggag cctgggaaaa cacaggaaat cccctacacg gtctttaacc gaacagataa
     5161 atcagtaaca gttgagacat tgatccactc cgctacgacg aattcaaacg gtgtgatcga
     5221 atatggagaa tcaaaaagta aatttgatga aacattaagt tatgatatga aaaacttagc
     5281 aaaaacagtg ggagaaataa ctgttcctcc tcaaagtgcg gtcactgaga cactttcact
     5341 gaaagcgcca gaaaaagagc tggctggtgt ggtcgcagga gggatcacgt ttcaagaaaa
     5401 aaaagagaac gtttctaaca aaagtacagt tggtgtcgtc aatcagtttt ctcaagtgaa
     5461 agcaatcttg atttgaaata gcgaaaaaga gtcttcccca gatttgaaat taaaaaaagc
     5521 ttatgcggat caaagcgatg gaagaaatgt gattaaggcg aatcttcaaa atccaaatgc
     5581 ttcttatatg ttcggcttga ctacatcggt tgaaatctac aaaaaagaaa caaaagagcc
     5641 ttacctttca ataaaaaaag aaaaaataga tgtagcaccc aatacaaatt ttgatttact
     5701 tgtcccatta gaaggaaaaa aattagctcc gggacattat caagcggttg tgaagggtgc
     5761 ttggaaagaa aaacgctgga atctccatac cgattttgaa ataactgatg atgcagcgga
     5821 ccaattgaat gaaaaggata ttgatttaaa aaatcagcca gaaacatttg actggcgatg
     5881 gctgatactt gttggagtca ttcttattgg cttatttgct ggattattta ttttaaaaga
     5941 aagaaggaaa aagaaatgaa aaaaacaatt gcattaagta ccgttctagc tggaagtcta
     6001 ttattatgta cacaaagtgt ttttgctgag aataagagct cagccactac agaaggaacg
     6061 atttctgtga ccaaaccaga tgctgcaaat cctgaagacg ttgttccagt tcctcctgtt
     6121 gatccaaatg atcctgatac tccttttaca cctgacgaac caaatcaggg aacatccgga
     6181 ttactgacga ttgaccaagc tccagatctt gattttggaa caatcaagtt aggtacagca
     6241 aaaacagtat atgctgcatt acaaagcgga acggacagta ctggagcaca aacacaagta
     6301 cagaatttcg tacaagtaac ggataaaagc ggaaattatg ccggttggaa attatcagtc
     6361 aaacgaacag aatttattgg tacaacatct gatacgaata aactaactgg ttcaaaggtg
     6421 aaattcaaaa acgccgtgat tcgaaatagc gcaactaata gcagtggaat gccatctacc
     6481 gtttctggaa ctggcgctga aggaatcgaa atcccagcag atcaggcagt agatctagta
     6541 actgcaggag caaatgaagg gattggcaca tggatttatt cattaggaga gaatgtggaa
     6601 caagggagac aatcagtgga attatttgtt cctgtatcca attatgctgt agacaactac
     6661 gtttcaacat tcacatggtc gttaacagat gctcctacag aagaggaagc gtaaaatcat
     6721 atcaacgact tcagggagtg gagccgattc aaggcccact ccctgaaatc tgctatgctt
     6781 acgtctcagc tcttattcat caactcgaag cagttttttg gaagtcatgg aaacgttcct
     6841 ttgtcttttc aaagatcttt cgaccaacga gtaaggagtc accgttgaaa aattctaaca
     6901 cataatgagc agaatcaatt ttctgcaatt gcgataagtt gatacagtaa gattttaaaa
     6961 gtaaaaagta agcatacttt tctaactctg tcttcaattg ttttaaagaa gtattcacta
     7021 atttatcttc tgtagttgtt ttgaccataa cttggttttt caaacctttg atactttcaa
     7081 tatataaaat ttcccgagga tacaggacga gacttccacc tttttgatca ataaagattt
     7141 tatcgacatt tacgatctgc tgttctttcc aattgatctg ttctagcact tgacgaattt
     7201 gttcagataa attcctgtct tttctgatat atccaatcgg aaggattttc cgattgatga
     7261 tctctactgc aaaattcgta taagcggtga aaaaaacaat attactatgt gcattttttt
     7321 tacgtatttg ttcggcgaca tcaatacctg tgagtgattg cttcaagtcg atatcgataa
     7381 agaaaacatc gtgattatca atagtgccca actggatcaa ctgattgatt tgagagatag
     7441 aagtgagctg cacgatattc tcgatatgat gattgaccaa ttctctgatg atccattcgc
     7501 tgtgaaactg gttatcttct atgataaaat actttttcat atgcaggctc ctattaaaaa
     7561 aatatgtgta cattttgaaa caaaacaatg gaggaataag atggtcgcaa cagtgacact
     7621 ttatttgacc tttactttga ttttagattt aggtatctat ttattctttt cgaaacaact
     7681 gctagtaaaa aacagctttg gttgtatagc tttgatggtg gtgacacttt tacttcctat
     7741 ccctaaagtg agctttcctt acctaattgg cttattattg atgtttcttt tttattttac
     7801 tacgaaaaac ctgtttatca gctgtttgat cagtaatctt ttttttgcta tcactggact
     7861 cagctttttt ctggtttttg atctgatgga ttatttagga aagagacatc ttggaacagc
     7921 aagttttttt gcactactta tcgcatttat tttctatcta ttagtaaaaa gtatagacaa
     7981 acagacgaga tttctcaact tgctttttac ttatccaact gaacactatt ttggaggtat
     8041 ttgtatttta ttaagctggg gcgtgattta cttggtattg ggaagtgttc acttccgctc
     8101 atctaattat ctgcttctat ctcttttgtc gatagtcatc agtttttttt ctttgttttt
     8161 ttccattatg ttgatcagtt tccaagtaaa agaaagacaa catcaggaat cgcttcaact
     8221 gtatcaaata aatgaagaat attaccatca tctagaggaa ttcaaacatg attataaaag
     8281 tctactgttt tcattaaaaa ttactcttca ggaaaataaa aaagaaacgt tggaattttt
     8341 agaaatatta gaagatcatt ctgcagctat ttttgaaaaa ccgcaaatac aacagctaac
     8401 aaatattgta tccccagctg tcaggggtgt ctttttaaaa tttctagaaa aagcagaatc
     8461 ggagaaaatc ccgatccaag tagttattcc tgtcgaagtg aaaacaatcc cgattgatct
     8521 ggttgttttt attcgctgtt tatctatttt tatttctaac gcattcgatc atcgtgtagc
     8581 cgatcagagt ccgattacga tcaatctgct ggaagaaaaa gagggatcac gttttagtat
     8641 agcgaacaag gcaaatccaa cgaatttgac gctatcggag atggtcaaaa gaggaaaaac
     8701 ctctaaaaaa aatggcgggt taggtctata cagtgcgaaa aaaatgttgg atgtttacga
     8761 aaatgcagaa ctaaaaatcg aattttcaaa acagaatcac tatttttttg tagaaatgta
     8821 tctggcatct agaaagagca aatcagccta ctgacttact gtcaaaggat aagtttgagg
     8881 aaagtctaca cggagataga gacaacaaca cagcatattc aaaagaaaca gaagcgtgat
     8941 gaccgttgtc acccttctgt ttcttttgct atgtagttaa acaaagtagt tgacacttcc
     9001 gctacttagt attagtacga aagtatactt catgatacta agtagataag aagtgaaaag
     9061 cggaacccac gatcgtcaaa atgctaactg ctttgcttac accagataaa gggaagagca
     9121 atatcgctta ttttaggcgt ggagaagcaa gcaaatccct acgatcacaa aaaaaatgct
     9181 gacccagcag ttaagagagt tagaacaaga tgaaagtatc catcgaaaag cctataacca
     9241 agtcccgcct aaagtagagt actcgctcac cgaagaagga aaatcattaa gagagattct
     9301 agtagcgttg tctgtttggg aagaagaacg tatcgaaaaa caaaaagcct caagggaaga
     9361 agacacgcta ctgcatacac atggtggcta cctaaattat tgattttcta tgacatttat
     9421 tttaaataaa agaaaacagg agttttttta aaagcatcat ctatttttta tgtaaaaaaa
     9481 tggtatgatt aatcaagtaa gaagtgattg gaagaaggca agcaaatgaa tagtatggaa
     9541 aaagtcaacc agtttagaga tgaacgtaat tggcgacaat ttcataacga aaaagatttg
     9601 gctatctcta tttctttgga agcatctgaa ttgttagaac tttttcaatg gaaaacgccg
     9661 gaagaagtaa aagaaacaca attagaacga atcaaagaag aactggcaga tgtattgatc
     9721 tattcataca tgatggcaga caaccttcac ctggatctag atgagatcat tgaagaaaaa
     9781 ttgatcaaaa ataatttgaa atatcctgta gaaaaaagcg caggtgtacg aaaaaaatac
     9841 acagagttgt agaaatgtgt cattcggctg ttggaaagag caaaagaaaa acagtaaaat
     9901 gagtctggag ggagagaaaa tgaaaaaaaa aggaaaagca aaaagatggc tagtcaatat
     9961 cctcttgttc ctcttgttat tagtaggact agctttgatt ttcaatgaac agatcaagga
    10021 ttattttgtc agagagacag gagataaata tgctatagcg aatgtaacaa aagaagatct
    10081 gaaaaagaat aacgataaag atgtcagttt tgactttgat gcggtagaac caatgacgac
    10141 agaaggggtc atgaggtcgc aaatgagagg aacagatcta ccagtgattg ctagtatcgc
    10201 agtcccttca gtttcaatca atttaccggt tttcaaaggc ttggataata cgtcactact
    10261 ttacggagca ggaacattat ctccagatca agaaatggga aaaggaaatt atgccttagc
    10321 aagccatcgt gcaacgaacc ctgaattgct gtttacaccg ctagaaaatt tagagatggg
    10381 tgccaagatc tatttgacag atttagaaaa tgtgtataca tacaaaacat tttttaaaga
    10441 aaaagttgct cctacagata cccaattact gaatgaagtc gagggaaaag aaattgtcac
    10501 actaattact tgtggcgaca tggacgccgt cacgcgacta gttgtacaag gagaactgga
    10561 aagtgttaca tcgataaaag atgcgacaga tgatatgaga tctgctttca atctagagac
    10621 aaaaacattt taggatactg ggacataagt cgttacagat taaaaacagc caaaggatat
    10681 gaatcgataa ggaaaaacca atttgctgaa aaaaagagaa acgcaaagag gtgcatctct
    10741 gatttggttg ttttctagat agacttgtgt tccagttttt ttatttttta tatgaaaagg
    10801 tttgcataaa taaaaattcg atggtatgat gattttgaat aagaaatgga ttgttactgt
    10861 acggcaggca aaaccagaat tcagctaaga aacagtaggc gaattctagg tttttttatt
    10921 tttcttgaga gggcgaagca aaatgaaaat tgataaaatt ttgaataaca atgtcgtcat
    10981 ttcgaaaaat ggattcgggg aagaagtcgt ttgtatggga aaaggactag cttttcaaaa
    11041 aaagattggg gacgagattt cgccagaagc ggtccaaaaa gaatttgttt taagagattc
    11101 ttttgctaaa aaccaattcc agcagctgat ggctgatatt ccggcagagg agatcgaatg
    11161 ggtcaaacaa gtagtcgaac tggctgaaga taaactaaaa gttgaatttt cgcctaatat
    11221 ttatctgaca ttgaccgatc atcttcatta tgcgatcaca cgagcaaaag aaaatatcca
    11281 actgcctaat ccattattat ttgaaacaaa aaaattttat ccgaaagaat atcaagcagc
    11341 gaaagaggct cttttgttcg tgaaacaaaa gacgggtgtg gaactttctg aggatgaagc
    11401 cggttttatt gcctttcatt ttgtgaatag ccaacaagga aacgaagaca tgcaagtgac
    11461 gatgaccgct accacaatgg taagagatat actgagtatc atcagtaaat tcttcggcat
    11521 cgtgttcgat gaagaatctt tgaattatca gcgtatcatt actcatctgc agttttttgc
    11581 ccagcgttat ctcaaagggg aaggatctga cgagcaagat gaatttctct acgcattagt
    11641 tcaaggaaag tatccaaaag cgtttcattg tgtcgaacgg atcaatgagt atcttcgaca
    11701 aacgcaaaaa caggaaatgg ggatcgcaga gcagatttat ttaacgattc atattcaaag
    11761 agtagtaagt gagaaaaaaa gcatccaatc ataaggattg ttactgtatg gcaggcaaaa
    11821 cctggattga aaaaataggt tcacgaatgt ttgtgactgt ttttttcaat ctgggttttt
    11881 attttttaaa taaaaaagaa agaaggaaaa aacaatggat aatcaagcag ttggtcgtcg
    11941 tgtatgggaa gcggtaggag gacaaaagaa tgtcaaaagt ttagtacatt gtgctacgcg
    12001 gttgcgattc agattaaaag atgaatcttt agcagatacg caaaagttaa aagaagaccc
    12061 ggacgtgatc caagtcgtac aaagtggcgg tcagtatcaa gtagtgattg gcagtaatgt
    12121 agcagatgtt tatcaatcga tcgtggatga ggaaggtctt gcagtttcgg aggatggaaa
    12181 agaagagtcc aaaaatattt tgaatcgtct gatcgatatt atctctagta tatttacacc
    12241 ttttctaggg gcgatggcag ccgccggggt attgaaagga ttcttatcat tggcaacggt
    12301 gatgggctgg ctgacagctg atagcggtac ttatcaaata ttgtttgctg cagcagatgg
    12361 tgtctttacc ttcttacctg tcatgcttgc ttttacagca gctaaaaagt tcaaagccaa
    12421 ccagttttta gcagtagcaa ttgctatggc acttgtgtat ccagctatca cagcagttgc
    12481 tggtgcggga gaagctatca gtttctttgg gatcccagtg attctttcac catctggtta
    12541 tacttcttct gtcataccaa tcattctagc cgtatgggtt caaagtaaat tagagccatt
    12601 cgtaaaaaaa gtgattccgc aatttctgca aatgatttta gttccgcttg tcgttttagt
    12661 agtgatggtt ccattaacat ttcttgcttt aggaccaatc gggacggttg ccggtaatgc
    12721 tttaggcggt ctcttcaact cgatttatgg atttagtcca atcgtagcag gtctaatcat
    12781 gggaagttta tggcaagtat tcgtgatgtt cggcatgcat tggggattcg taccgatcat
    12841 gtttttgaat atcgaacaat atgggtttga tgtattgatg ccaatgcttc ttccagccat
    12901 cttggcacaa ggtggggctg ctttagctgt agcattgcga acaaaagata cgaaacttcg
    12961 agcattaggg atttcttcta cagtgacttc gttatttgga atcactgaac caactgtgta
    13021 tggtgtgaca ttacctttga agaaaccatt cattgccgct tgtatttctg gaggtattgg
    13081 tggagctatt atcggatttt ccggagtaaa agctttctcc agcagtttgg tcagcttatt
    13141 gacgattccg actttcatta atacggtgga tggtgtagag tcaaatgtga cagtagcagt
    13201 aatagctaca ggaattgctt ttgttcttgc attcgtaggt acattgattt taggatttga
    13261 cgaacaaaca caagacaatc aattagaaaa caaacatgcg aatgcaggag aaccgattac
    13321 ttcagcacgg cataccttaa aaagtccttt aacgggtaaa gtactgccat tgagtgaagt
    13381 accagaccaa gttttttctt ctggcgtgat gggaaaaggt atcgctattg atccagaagt
    13441 aggagaacta gttgcacctg cagacggaga gatcacgact atttttccaa ctggacatgc
    13501 tgtagggatc acaacgactg acggagcgga gattttgatt catatcggga tggatacagt
    13561 agaattaaat gggaatggct ttgagatctt agtcaaacaa ggggatctcg tcaaagcagg
    13621 agatttattg attcgttttg acatcgaagc aattagagca gctggctata gcgtaatcac
    13681 tccagttgtc attacgaata ctgatgcatt tgccgatata cttgaattag accaaaaaga
    13741 gattattgca aacgaagatg ttctggcaat tgtaaaataa ggaggaaatc aggatgcaat
    13801 cacgtttttc agaaaaattt ttatggggtg gagcaactgc tgccaatcaa ttagaagggg
    13861 cttatgatcg agacggaaaa gggctatccg ttgcagatgc tatgcctgga ggtaagcagc
    13921 gctttgcgat tatcggcagt gaagaatttg actggacgat tgatcaagaa aaatatattt
    13981 acccgaatca tcgaggaatt gatcattacg atcgtttcaa agaagatctt gccttgtttg
    14041 cggaaatggg attcaaatgt tatcgttttt cgattgcctg gacacgaatt tttccaaatg
    14101 gagacgaagg tactcctaac gaagcgggat tagaatttta tgatcagctg attgatgagt
    14161 gcttaaaata tgatattgaa cctgtgatca cgatttctca ttacgaaatg cctcttcatt
    14221 tggcaaaaga atacggtggc tggaaaaatc gaaaactgat cgacttctat gaacgatttg
    14281 ctcagacagt attagagaga tacagcagta aagtcaaata ttggatgact ttcaatgaaa
    14341 tcaattcggc ctttcacttt ccagctttaa gtcaaggatt agtaaaaagc aatggagcag
    14401 gagagtacca aaatattttc caagcatggc ataatcaatt cgttgcaagt agcaaagccg
    14461 taaaaatcgg ccacgaattg cgttcggata tccaaatcgg ctgcatgatt atctatgcaa
    14521 caacttacag catcgatgcc aatccagtca atcaggcagc aacaatgatc caaaaccaag
    14581 aattcaattt tttctgcacg gatgtccaag tacgtggaga ataccctgca tacacagcgc
    14641 gtacccataa aaaatatgga gtcgatccgg aaaaattaga gcaaacggaa aaagacttcc
    14701 agttgctaaa agaatatcca gtggactaca tcggatttag ttattatatg tctacggcaa
    14761 tcaatgagac tgatccacaa gcagcaactt cagaagggaa cttgctggga ggagtaaaaa
    14821 atccgtttct tgaggcaagt gaatggggct ggcagatcga tccagaaggg ctgagaatcg
    14881 cattgaatga attatacaat cgttaccaga aaccattatt cattgttgaa aacggtcttg
    14941 gagccattga tcatatggaa gaggatggca cgattattga tgattatcgg atcgattatt
    15001 tacgtcgtca tatcgaagcg atggctaatg cagtagcaga cggtgtagat ttgatgggct
    15061 atacgccttg gggatgtatg gatcttgtca gtgcatcaac aggagaaatg agtaaacgtt
    15121 acggctttat ctatgtggat ctagacgatg aaggaaatgg gacactgaat cgatcaaaga
    15181 aaaaatcttt tgattggtat aaagaagtaa ttgcctcaaa tggcgtaaac ttgtaattaa
    15241 agcagctgaa tgaaaaagaa cacacttctc acatgtgatt tgtgttcttt ttttattttt
    15301 tatgtgctat tcttttagta atagtaaagc aaagggtgac gttatgaaaa ggacacgtgt
    15361 actatacttg gaagtcgcag ataaaattaa agaagatatt ttctcaggaa aatatccagt
    15421 cggttcgatg ttaccgacag aaagtgaatt agaagaactg tttcaagtca gcaaaatcac
    15481 tgtccgaaag gcaatcgaac tgctggcatc agatgaatat gtcgcaaaga aaagtggtcg
    15541 tgggacgacg gttctaagca atcgtcctta caatcgatta tccaaagcgg caagctttac
    15601 gcaaatcttg gagaactccc gatatgaagt ccggaaagaa acgctggatt tgcaattggt
    15661 ccatataaag aaggaagatc ctttgttttc ctttttcggt gagcaagctg tttgttttaa
    15721 gcgcttatat tatttgaaca atcagcctta tatttatttt gttcattatc ttccacaaga
    15781 aatgatgcaa ttcagcagag aagaatttgg caaagaatct ttgtatcgtt tgctgacgaa
    15841 aaaaggatat actattgact cattttctga tgatttctcg gcagtatttt taacagaaga
    15901 agaacaagaa attctgaaaa cagacgcaca gatcggcatc aaacggatac ggaaatcgtc
    15961 tacgcaaatt ggggaaatga ttgaatattc ggaggcggtc taccatacgg agctgcatcc
    16021 ttaccatatt gattatgaaa cataaataaa aaaagaggtt gtgaaaaagc tgccctgcat
    16081 caagtaataa gaacagccaa acaaaaatag cttctcatat tagcttctca tatttttcgt
    16141 ttggttaggc ttattaccgc agatacccgg ctgtttctgg attaccattt attctggata
    16201 agggggtgtg gcacgatttt cagaaggaat ctatgaacca atcactgtat tgattttagc
    16261 ttcgatggta accccattcg gcttgacgat cgcttatttc ttagggaaaa tcattcgtaa
    16321 aaatatattc aatcgtcaag aaatcgatac attgaagacc gcttttccta tggggatctg
    16381 tcagatcaca gagggatgtt tccctatcgt attgaacgac ttggcacgca atgtcatagc
    16441 aacgggagtc ggtggggcag tcggcggtgg attgagcatg ttttggggcg cagatagccg
    16501 tattccggca tcgggaatgt ttgcagtcgc tacgatgacc agaccatggg cattcattgg
    16561 tgcattgtta gctggttctt ttgctacggg aataactatt ttattattga aaaaaccagt
    16621 tgacccaaat gcagaagtca tccaagaaga aaaagaggaa gaagagatat cttgggatga
    16681 cttgacaatc agttaggagg aacagaatgg attacaagaa aagagtacaa caaatgttcc
    16741 tagattcagg agtagtattg aaagaaacag aattagaaag aatcgaatat acagattttg
    16801 gattaggagc aatcgagact gaaggattga atttatttgt ttatgaaaat aatgagcgat
    16861 actgtgggaa agaactggta ttattgcctt atcaaacttg tccagagcat cggcacccac
    16921 ctcgagatga gaatgaagga aaaaaggaaa cctttcgttg tcgaaaaggc ttagtatatc
    16981 tttatgtcga aggaaaagaa acaaaaaaac tttcctgtat tattccaaag gggcaagaac
    17041 gttactatac ggcaaaaaaa gaaatcatac ttcacgctgg agatcagtac acgatcgaac
    17101 aaaatattaa acactggttc caggcaggtg caacaggcgc ggttatcacc gaattttctt
    17161 ccagcagtga tgatccaagt gatagtttta cgaatccagc tatttatcgt taatacaaag
    17221 gtttgggaac aagctgatga accaaaatga tcaaatttcg attatttggt tcgtcggctt
    17281 ttttatttag agaaacatca ggcgttggtg aaacataaca cgagaataaa atggattttt
    17341 cattaatagg agaaacaagt gtgtgactat ttaaataatg cgtgttattg acatgcagga
    17401 cgaaatccca atttcttcta taagtaagtc gtttcaattt tatatttact agttatttat
    17461 ttttttggtt actgtaaaat agctgaaaaa ttattttttt gcaaatacta tatatagtgg
    17521 tgtttgatct atataccact aaaaaactac aaaatctagt cttttatttt aaaaaaacac
    17581 gtgtagaatg aagatagact tttgagttag ggagtgtttt tttatgaaac ttactgtttt
    17641 ttgcggttcc cgttttggca ataaagaaag ttacaaattt attgcgcaaa cgttaggaaa
    17701 atatatggca caagaagaaa tcgaactggt ttacggtggt tcatactccg ggattatggg
    17761 agtgattagt aaaacagtac tggaaaataa cggaaaagtc acaggtatct atccaaatgg
    17821 cctttttgaa actgagctgc ctgggaaaga tgttactaca tttattccta cagagacaat
    17881 tgatgaaaga aaggtattat tatttgaaaa aggagatgct gtcctcgttt tccctggagg
    17941 tctaggtaca ttggaggaat tttcgcaact tctttcttgg atagcgatcg gcctgacacc
    18001 tgataagcca atcggaatat tgaatattgg cggttattac agcggcttgc aacaattatt
    18061 agaaactttt gcaaaagagg attttatgga taaaaaatgg ttggatcaag tgatcttttc
    18121 caacaatcca ttggaactgg tcaatgaatt acgagcagta tctattaaaa atcagttatt
    18181 attgaaggag gcaaaataat gagtcaaata tatttagcag gaccattttt ttcagaggag
    18241 caaatcgatc gagtaagtcg tatcgaaaaa gcgttggagg aaaacaaaac agtcacaagt
    18301 ttctactctc cacgtcatca ccaagaaagc aattatgaac tattcagcgc tggatgggcg
    18361 caggaagtat acgaaaaaga tatggaagag ttgacaaatg cagaatttgt tttggctatc
    18421 cttgactttg agcatcaaac aatcgatcca ggaacggctt acgaattagg tgtggcaact
    18481 atgttgaaaa aaccgatgat catcgtacaa gaagagacgg tacctacgaa cttgatgatt
    18541 acacaaagtc ttcacacgta tttaaaatca gaccaagctg tacgtgaata tgattttgaa
    18601 aaattgcctg ttgaaactta cgtaggcgag tacttataga agaggaaggg ttgtatgtca
    18661 gtgattttga tttcaggaac gattggtgca ggtaagagca gtttgacgga tatgcttgca
    18721 aaagagattg attctaaacc attttatgaa aatgtcgaag ataacgaagt attgccgctt
    18781 ttttattcaa atcctgagca gtacgcattt ttattgcaaa tcttcttttt gaataagcga
    18841 tttttagcaa tgaaaaatgc tttagtgaac gatgacaatg tgctagaccg ctccatctac
    18901 gaagatagtc tgttgttcca tctaaatgct gatttaggtc gggtaacgga tatcgaagta
    18961 cagcagtacg atagcttatt agatacgatg ctaaacgagt tagatgatgt tgctccaaaa
    19021 aaacgtcctg atttgatggt ccatatcaaa gtttctttgg acacgatgct agaacgtatc
    19081 aaaaaacgcg ggcgtgacta tgaacagcta gaaagcgatg aaacgttata cacctattac
    19141 gaaacactga acacccgcta taatcaatgg tatgaggatt ttgatgtatg tcctaaaata
    19201 caagtggatg gggatacatt cgattttgta gaaaatccag aagatgcaaa aaaggtgatt
    19261 cgtcagatcc aagaaaaatt agctgaattg gaaggaaagc ctgctggaaa atattttaca
    19321 gataatcagc atgcgaaagg tcaagaatta ggctaaaagt aaaaaaccaa acgacgggaa
    19381 agaaaaatta ctacgtgatg tagaataa
//
LOCUS       DAZKKW010000051        19094 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.051, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000051 DAZKKW010000000
VERSION     DAZKKW010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19094
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.051"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            30..2183
                     /locus_tag="ACG8PO_001732"
     CDS             30..2183
                     /locus_tag="ACG8PO_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0043214 - ABC-type bacteriocin transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HFQ0207042.1"
                     /translation="MIKSFPFVQQQDEKDCGVACLSMILKYFKTDIPIHKLRDLSGTD
                     LEGTSAFGLKKTFEKLSFDCMAIQADSEIWKERDLILPLIAHILVDNSYMHYVVIYKT
                     KGNVLWIADPANGKNRKTIEAFQKEWTGILLLPMPRETYIPIKEKVAGLSSFLPIIWK
                     QKGLVFHIVLASIFITFFGIGSSYYFQGILDFFIPNQARSTLNIISIGLIAVYLFRVL
                     FEYSRSYLLIILGQRMSMAIMLQYFKHVLTLPMNFFATRKSGEIISRFLDASKIIDAL
                     ASATLSIFLDIGMVLLVGVTLAIQNSTLFLITLCSLPFYVVAIFAFVKSYEKSNREEM
                     AAGAAVNSSIIESLKGIETIKAYSGEEKVYNRIDHEFVKLMKKSFRTVTLDNIQQGIK
                     HLIQLISSALILWLGSYFVMDNRISLGQLITYNALLVFLTDPLQNIINLQVKMQTAQV
                     ANNRLNEIFAIDSEKSNQSFEYMLSEQLFQQDITVKNVSFSYSMKAPTLNEISCTIPS
                     HSKTAIVGVSGSGKSTLAKLFVRFYEASEGSIHYGNINHLDIPFQILRDRVTYLPQES
                     FFFSGTIIENLIFGLDSPPSFEQIIEVCEIVQLNEFINQQPLRYETLLEEGGNNLSGG
                     QRQRLALARALLKNPDILILDEATSGLDTLLEHAIMNYLLNLKEKTIIFIAHHLPIAK
                     ACDQILVLHEGHLVEKGTHDELRFADGIYQKLWEI"
     gene            2152..3567
                     /locus_tag="ACG8PO_001733"
     CDS             2152..3567
                     /locus_tag="ACG8PO_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFQ0207043.1"
                     /translation="MVSIKNYGKSRRGKQFMQNNNWFEHSSIYTKQHHSFYRWVLYPV
                     ILFLCIIGLFLTFAKKEVVIRTTAQLNPEKIEKLQVPLEAKIMENFLKENQFVHKDDV
                     LVRLDCSLIENEKAQIEQENQRITQQIKMAQLFIESISKGKNLFSTDDSFGYSNQLKS
                     MLSEKESLRYALKQSELNDQKQLEVYEKTKRQLEKQIESSDSKLQEWQQVQVAWSNNQ
                     SLKDFSKEMMANYENWQEQLNNVSDDQKNQVKLTISASINEQIEQLKKEVEQYQSEKA
                     KLVKPTTSENDRISQTEKGKQELEQAITTVKQTVTELNEKQEKNQSVIKSLDDQLSKG
                     ILKAPVSGTVHLNEETKGQAELAKGTVLAEIYPIHEKSKMKFTALLPANESTYIKEGM
                     KVHFKLDQKGGAPITIDGTLDEISENSTATEKGVFYAVKGTLKTTQSFPFRYGLTGEL
                     SLIVGEKTYWQQIVDVFLNKK"
     gene            3600..4634
                     /gene="sppA"
                     /locus_tag="ACG8PO_001734"
     CDS             3600..4634
                     /gene="sppA"
                     /locus_tag="ACG8PO_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFQ0207044.1"
                     /translation="MNKRRWIAVGVACGLLLLSIIVALIPGKDKEEASNTTLTGINKI
                     FYGSNEITEETLEEGASNKKIVKLSVNGVIADTGESNLFSREQYNHQNFLTQLKKIQE
                     DKAVKGVLLEVNSPGGGIYESAEIAKEMAKIKKLDIPIYTAFKNTAASGGYYISAGSD
                     KIFATEETTTGSIGVIISGLNYSGLLEKLGVTDATYKSGALKDMMSPQHKPSEEENKV
                     IQEFVMSAYDRFVNVVAKGRNMDTNAVKELADGRIYDGNQAVENGLVDQIGYSEDALD
                     SLKKEKKLTDATVIEYKNDTTGFASSWLGNKIAEWQGLKATTSDRVISVFEKLGFSDT
                     PKPMYYYGGL"
     gene            4636..5157
                     /locus_tag="ACG8PO_001735"
     CDS             4636..5157
                     /locus_tag="ACG8PO_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HFQ0207045.1"
                     /translation="MNKKSGIETLPNYVFAGFWLRFFAFLIDLICISIITHATIGLFY
                     KIMGLESDKSIITFYGIASLAIYLTYFVLLTKLNSGQTIGKMIFGIRVVSFEEKELSW
                     QTIFIRECICRFILKNFVFQIGYLVAAFTPKKQHIGDYFSNTSVVVIRSLEAINYGQK
                     ISNSKTLNLERIK"
     gene            5154..5540
                     /locus_tag="ACG8PO_001736"
     CDS             5154..5540
                     /locus_tag="ACG8PO_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HFQ0207046.1"
                     /translation="MNKRKTEFILILISGITGILCSLLALMGGMFLSSIDSTTLNTTE
                     LTSKDIDMLVNLSGNVVLFGGITLVFSIMIFIIGFLVRKNQKTVVYGISLLILSIIPF
                     FLLTLLWVIPGVLGIIAGLMLLIRKS"
     gene            complement(6036..6108)
                     /locus_tag="ACG8PO_001737"
     tRNA            complement(6036..6108)
                     /locus_tag="ACG8PO_001737"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6073..6075),aa:Lys,seq:ctt)
     gene            6489..7058
                     /locus_tag="ACG8PO_001738"
     CDS             6489..7058
                     /locus_tag="ACG8PO_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HFQ0207047.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            7045..7905
                     /locus_tag="ACG8PO_001739"
     CDS             7045..7905
                     /locus_tag="ACG8PO_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFQ0207048.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITPEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            8095..8799
                     /gene="yycF"
                     /locus_tag="ACG8PO_001740"
     CDS             8095..8799
                     /gene="yycF"
                     /locus_tag="ACG8PO_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator YycF"
                     /protein_id="HFQ0207049.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            8804..10639
                     /gene="walK"
                     /locus_tag="ACG8PO_001741"
     CDS             8804..10639
                     /gene="walK"
                     /locus_tag="ACG8PO_001741"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HFQ0207050.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVILSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            10636..11952
                     /gene="yycH"
                     /locus_tag="ACG8PO_001742"
     CDS             10636..11952
                     /gene="yycH"
                     /locus_tag="ACG8PO_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system activity regulator YycH"
                     /protein_id="HFQ0207051.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            11953..12822
                     /locus_tag="ACG8PO_001743"
     CDS             11953..12822
                     /locus_tag="ACG8PO_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HFQ0207052.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            12877..13686
                     /locus_tag="ACG8PO_001744"
     CDS             12877..13686
                     /locus_tag="ACG8PO_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFQ0207053.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(13789..14418)
                     /locus_tag="ACG8PO_001745"
     CDS             complement(13789..14418)
                     /locus_tag="ACG8PO_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFQ0207054.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAASDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            14588..15880
                     /locus_tag="ACG8PO_001746"
     CDS             14588..15880
                     /locus_tag="ACG8PO_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFQ0207055.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQHLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(15956..16618)
                     /locus_tag="ACG8PO_001747"
     CDS             complement(15956..16618)
                     /locus_tag="ACG8PO_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HFQ0207056.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            16843..17127
                     /gene="groES"
                     /locus_tag="ACG8PO_001748"
     CDS             16843..17127
                     /gene="groES"
                     /locus_tag="ACG8PO_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFQ0207057.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            17178..18803
                     /gene="groL"
                     /locus_tag="ACG8PO_001749"
     CDS             17178..18803
                     /gene="groL"
                     /locus_tag="ACG8PO_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFQ0207058.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDATIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
BASE COUNT         6496 a         3071 c         3947 g         5580 t
ORIGIN      
        1 aattttttag aaggagttgt gttagataag tgataaaatc atttcctttt gtgcagcaac
       61 aagatgaaaa agattgtggt gttgcttgcc tttcgatgat tttaaaatat tttaaaactg
      121 atatcccgat tcataaatta cgggacctat ctgggactga tttagagggt acttcagcat
      181 ttggattgaa aaagacattt gaaaaattgt cttttgattg tatggctatt caagcagata
      241 gtgagatctg gaaagaaaga gatcttatct taccattaat tgcacatata ttggttgata
      301 acagctatat gcactacgtt gtaatttata aaacgaaggg aaacgtacta tggattgcag
      361 atcccgctaa tggaaaaaat agaaaaacga ttgaagcgtt tcaaaaagaa tggacaggta
      421 ttcttttatt acctatgcct agagaaactt atattcctat taaagaaaaa gtagctggat
      481 taagttcttt tttgcctata atttggaaac aaaaaggatt agtattccac atcgttttag
      541 catcgatttt cattacgttt tttggcatag gaagctcata ttattttcaa ggtattcttg
      601 attttttcat ccctaatcaa gcacgttcaa ctttaaatat aatttccatt ggcttgatcg
      661 ctgtctattt atttcgggta ttgtttgaat atagtcgtag ttatcttctt attattttag
      721 gacagcgaat gagtatggca attatgcttc agtatttcaa acatgtgtta actttaccaa
      781 tgaatttttt tgctacgaga aaatcagggg aaattatttc tcgttttctt gatgccagta
      841 agattattga tgctttagca agtgctacgt tatcaatatt tttagatatt ggtatggtgt
      901 tgcttgttgg ggttacgtta gctattcaga atagtacgtt gtttttaatc actctatgct
      961 ctttgccttt ttatgtagtt gcaatattcg ctttcgtgaa aagctatgaa aaatcgaaca
     1021 gggaagaaat ggcagccggt gcagctgtaa attctagtat catagaaagt cttaaaggaa
     1081 tagaaacaat taaagcatat agcggtgaag agaaagtata taaccgtata gaccatgaat
     1141 ttgttaaatt aatgaaaaaa tcatttcgta cggttacgct tgataatatt caacaaggaa
     1201 ttaaacattt gattcaatta attagcagtg cattaatttt atggctaggt tcttattttg
     1261 ttatggataa tagaattagt ctaggtcaac ttatcacata caatgcttta ctagtatttt
     1321 tgacagatcc tttacaaaac attattaatt tacaagtgaa aatgcaaacg gctcaagttg
     1381 caaataatcg tttaaatgaa attttcgcga ttgattcaga aaaatcgaac cagtcttttg
     1441 aatatatgct ttctgaacaa ttatttcaac aagatatcac tgtaaaaaac gtttcattct
     1501 cgtatagcat gaaggcccca acgttgaacg aaatttcatg tacaattcct tcgcacagta
     1561 aaacggctat agttggagtt agtggttctg gaaaatcaac tcttgcaaag ttatttgttc
     1621 gtttttatga agcatcagaa ggatcgatcc attatggaaa cattaatcac ttagacattc
     1681 cttttcagat attacgcgat cgagtgacat atttgcctca agagtcattt ttctttagtg
     1741 gaacaatcat tgagaatctt atattcggtt tagatagccc accaagtttc gaacaaataa
     1801 tagaagtttg tgagatagtc caactaaatg aatttattaa tcaacagcca ttacgttacg
     1861 agacattgtt ggaagaaggt ggaaataatc tttcaggagg gcaacgacaa cgattagcct
     1921 tagcacgggc tttattaaaa aatccggaca tattaatttt agatgaagca actagtggat
     1981 tagatacctt gttagaacat gcaattatga attatctcct caatttaaaa gaaaaaacga
     2041 ttatttttat tgcgcatcat ttaccaatag caaaagcatg tgatcaaatt ttagttctcc
     2101 atgaagggca tttggtagaa aaaggaacgc acgatgaact tcgttttgcc gatggtatct
     2161 atcaaaaatt atgggaaatc taggagaggg aaacaattta tgcagaataa taattggttc
     2221 gaacattcta gtatctatac taaacaacat catagcttct accgttgggt gttataccct
     2281 gtaattcttt ttttatgtat tattggattg tttttaacct ttgctaaaaa agaggttgtt
     2341 attcggacaa cggctcaatt gaaccccgaa aaaatagaga agttacaagt gccattagaa
     2401 gctaaaataa tggaaaactt cctaaaagaa aatcaatttg ttcataagga cgatgtgttg
     2461 gttagattgg attgttcttt aatagagaat gaaaaggctc agatagaaca agaaaaccaa
     2521 cgtattactc aacaaataaa gatggctcag ctatttattg aaagtataag taaaggaaaa
     2581 aatttgtttt caacggatga cagttttggc tacagtaatc aattaaagag catgttgtca
     2641 gaaaaagaat cactccgcta cgctttgaag caaagtgaat taaatgatca aaagcaatta
     2701 gaagtatacg aaaagacaaa aagacaacta gaaaaacaaa ttgagagttc agatagtaaa
     2761 ttacaagaat ggcaacaagt acaggtagct tggagtaata atcaatcatt aaaagatttt
     2821 tcaaaagaaa tgatggcaaa ctatgagaat tggcaagaac aactaaataa tgtttctgat
     2881 gatcaaaaaa atcaagtgaa actgacaatt tcagcaagca taaatgaaca aattgagcaa
     2941 ctaaaaaaag aagtagaaca gtatcagtca gaaaaagcta aattagttaa accaactact
     3001 tctgagaatg acagaattag tcaaacggaa aaaggaaagc aagagctaga acaagcaata
     3061 acaactgtaa aacaaacagt tacagagcta aatgaaaaac aagagaagaa ccagtcagtt
     3121 atcaaaagtt tggacgatca actttcaaaa ggaatactaa aagctcctgt atctggaacg
     3181 gttcatttaa atgaagaaac aaagggacaa gcagaactag ccaaaggaac agtgcttgca
     3241 gaaatttatc cgatccacga aaaatcaaaa atgaaattta cagcactttt acctgcgaat
     3301 gaaagtacct atattaaaga agggatgaag gttcatttta aattggatca aaaaggtggg
     3361 gctccgatta cgatcgacgg aactttagat gaaatttcag aaaattctac tgccactgag
     3421 aaaggtgttt tttatgcagt gaaaggaacg ttaaaaacaa ctcaatcgtt tccgtttcgt
     3481 tatggattaa ctggagagtt gtctttaatt gtaggagaaa aaacatattg gcaacaaatt
     3541 gtcgatgtgt ttctaaataa aaaataatta tttttactaa gaatatagga ggagtttata
     3601 tgaataaaag aagatggatt gctgtgggag tagcgtgcgg attattgtta ctatccatta
     3661 tcgtcgcgct aattccagga aaagataaag aggaagcatc aaatacaacc ctaacaggaa
     3721 taaataagat tttttacgga tctaacgaaa tcacagaaga aacgttagaa gaaggcgcat
     3781 caaataaaaa gattgtcaaa ctatccgtca atggagttat tgcagataca ggtgaaagca
     3841 atctttttag tagggaacaa tataaccatc agaatttttt aacacagcta aagaaaattc
     3901 aagaagacaa agcggttaag ggagtgttat tagaagtaaa ttcacctggg ggaggaatat
     3961 atgaaagtgc tgagattgct aaagaaatgg caaaaattaa aaagctagat attcctatct
     4021 atactgcatt taaaaataca gcggcaagtg gagggtacta catttctgct ggttcagata
     4081 aaatatttgc tacggaagaa acaacaactg gatcaattgg tgtcattatt tctggtttaa
     4141 attattctgg cttattagaa aaacttggcg taactgatgc gacgtataaa agtggtgcat
     4201 taaaagatat gatgtcacct caacacaaac cttcagagga agagaataaa gttatacagg
     4261 aatttgtgat gagtgcttat gatcgttttg tcaatgttgt agcaaaagga agaaatatgg
     4321 atactaatgc agtgaaagaa cttgcggatg gaagaattta tgatggtaac caagcagtag
     4381 aaaatgggtt ggtggatcaa attggttact ctgaagatgc attagattcc ctaaaaaaag
     4441 agaagaaatt aactgatgcg acggttatag aatataaaaa tgacacaact ggctttgctt
     4501 cttcttggtt aggtaataag attgctgaat ggcaagggct aaaggcaact acatcagatc
     4561 gtgtaatttc agtttttgaa aaacttggat tttctgatac tccaaaacca atgtattact
     4621 atggagggct ataaaatgaa taagaagagt ggcattgaaa ctttgcctaa ttatgtattt
     4681 gcgggatttt ggttacgatt ttttgctttt cttattgatt taatttgtat tagcatcatc
     4741 acccatgcta ctataggtct cttttacaaa atcatgggac tggaatctga caaatccatt
     4801 attacttttt atggtatagc gtcattagct atttatttaa cctattttgt acttttgact
     4861 aagctgaatt caggacaaac aattgggaaa atgatttttg ggataagagt agtgagcttt
     4921 gaggaaaaag agctaagttg gcaaactatc tttattcgtg agtgtatatg taggtttata
     4981 ttaaaaaatt ttgtatttca aattgggtat ttggtggctg ctttcacgcc caagaaacaa
     5041 catattgggg attatttttc taacacgtca gtggttgtta ttcgttcatt agaagctatt
     5101 aactatggtc aaaaaatttc taattctaaa acacttaatc tggagagaat taaatgaata
     5161 agagaaaaac agagtttatt ttgattttaa ttagtggaat aacagggatt ttatgtagtc
     5221 ttttagcatt gatgggtggt atgtttctat cttctattga ttcaactact ttaaatacta
     5281 cagaactaac cagtaaggat atagatatgt tagtcaatct aagcgggaat gttgttttat
     5341 ttggaggaat taccctagtt ttttcgataa tgattttcat aataggtttt ttagtaagaa
     5401 aaaatcaaaa aacagtggtc tatggtatta gtttactcat tttatctatt attccatttt
     5461 ttcttttaac attattgtgg gtaattcctg gagttttagg tattattgcg ggattgatgc
     5521 ttctaataag aaaatcttaa ttaggcaatg tgtagacaac gtgaaacgat taatcaatta
     5581 aaagctaaat acgttttgag aaatgataac ttgtagtcga tggaagacat gtagataaaa
     5641 aaactacgtg tcttttattt attctaattt tttcgaaaaa aagacactct ctgattgttg
     5701 tattaatgga aaaggggaaa gcagcaagta aatttagttt ttctattaaa tattcttcac
     5761 tttttaacct tttatagtac ttagatttaa tcctatatgt ttagagagtt cattggcagc
     5821 atgattttgc aaagcttctc gaattattgt tatattagaa actttactgt tcataattat
     5881 gcttcttttt taactcaaag tccaaagggc tacagtggac tttgagttaa aaataaaaag
     5941 ccctagttat tttgaaaaat aaaaaaagaa cagcgtaaat gttttcgaaa tgctttcgca
     6001 ccaacattta cctgttccgt gggtttggca cccaatgggc cgtgaggggc tcgaacccgc
     6061 gacccgctga ttaagagtca gctgctctac caactgagct aacggcccgt acaaaaagta
     6121 cttaataata gtagcactaa gaattttaaa atgcaagact acatttaaag aaaatcagaa
     6181 aaatctttac ttccttatag gaaatcattc atagattatt cacatttgct cgctattata
     6241 taagcatacc aacaaacaaa gttcttgcaa gagaacaaac cctaacactt ttttcatttt
     6301 ttactccttt cccgccgtct ccccggcggg tctcctgttc ttaacgataa aagacaagaa
     6361 ggcttagcct tcttgttttt tgttttcata gaatcccgct aatcaaatca cctagttaaa
     6421 gcaactagat tgaatgttta tcataagtct gatatcatgt ttatgaaatg aaaaaaggag
     6481 cggttatgat ggaaaaaatg cgtcagcaat taatggagtg ggggaaggaa ctagaaacat
     6541 tccgtctgcc tcactgggaa gaactccctg acttagaact ttatatggac caagtcagaa
     6601 cgttagttga tcgttatctt tcacctgtga tacaaggaga aaagcatcca ttgcttacct
     6661 cttctatggt aaacaattac gttaaactag gtttgatacc agcaccagtt aaaaaaagat
     6721 ataacaaaga acacgtagca tttttacttg caatcactac gttgaaacaa gtattgacta
     6781 ttcctgaaat caaagaaggg atattgttcc aaggaaaaac agtaggaatt cgtgaagcct
     6841 ataatctatt ttgcgatgaa caagaagcag ctgtgtggat ggtcagccaa ttagcacaag
     6901 gtaaatctca tccgcaaaaa ttcgaccagg caacccctgt agaatatatt gctgtaagag
     6961 cggcaacttt atcgtttgca atgaaacttc ttgcggaaaa aacgattgtt ctagaaaccg
     7021 aatatttaaa agaggagaaa acaaatgaaa aacaataaaa tcgcaatttt agtagattca
     7081 ggtacggatg ttccagaaga attgataaaa aaatacgaga tgtatatgat cccattgaaa
     7141 attatttata aagatcaggt atatacagat aaaattgaca ttacccctga agagatctat
     7201 caacgtttgc cagaggaaat ccctggtaca tctttgccgg atggtgagtc gatcacagaa
     7261 atctttgagc aaatcaaaaa agacggttat gaaaaggtac ttgcggtaac aatttccagt
     7321 ggattgagcg ggacatataa tgtggttcgt ttaattgcac aacagcaaga agaattggaa
     7381 gcatatgtac tagatacgaa aaatattggt attggtgcag gtttttctgc gatccaagca
     7441 gcaaaatggc tggaagaagg agtagaatgg gatcaactga tcagtaatct gaatgagtta
     7501 gtcgaaagaa caaaagtttt ctttaatgtt gcgacattgg aatatcttca aaaaggtgga
     7561 cggatcggtt tagttgcttc tattgtagga accgctttaa agcttaaccc aatcatctca
     7621 tgtaatgaag aagggattta ttacacagta ggaaaagcta ggggacgtaa aaaaagtttg
     7681 gataaaacgg ttgcttacgt caaagaaaaa atcggtaatg caaaggtctt caacttagct
     7741 gttgcccatg gggatgcgaa agaagaagca attgaaatgg aagcgagact taaaagagag
     7801 ttcccacaag caaaccaaat ctactttggc cagatttctc cggcattagt agtccacact
     7861 ggtccgggat tattgggtgt cggtgtacaa gttttagaag gataaatcaa aaaaaggaaa
     7921 cactgaggtg aaaatcagtg ttttcctttt ttattgaagt tagaacaaaa aataataaag
     7981 tattttgcac tttcatttta attttctctt ttgttttata ggcaaagatg aaacaaaagc
     8041 agaaatacgg tataatgaga aggtatgaca ataatatata agaggtggct ctttatgaaa
     8101 aaagttttag ttgtcgatga tgagaagccg atttcggata tcgtcaaatt caatttagcc
     8161 aaagaaggct atgacgttta tactgcatat gatggggaag aagccttgga aaaagttgct
     8221 gaggtagaac ctgatttgat tttacttgat ttgatgctac ctaaaatgga cggcttagaa
     8281 gttgctcgtg aggtacgcaa aacttatgat atgccgatca ttatggtaac agccaaagat
     8341 tctgaaattg ataaagtcct aggcttagag ttaggcgccg atgattacgt gaccaagcca
     8401 ttttccaacc gagaattagt tgcacgtgtc aaagcaaatc ttcgtcgcgg tgccacggct
     8461 gcgaaggaac cagaagaggc agcacctgcg gaattgacga ttggtgattt gacgatccat
     8521 ccagaagctt acatggtgac gaaacgagga gaaacaatcg agttaacaca tcgtgaattc
     8581 gaactactct tttacttggc gaagcacctt ggacaagtga tgacaagaga acatttatta
     8641 caaacggttt ggggttatga ttattttgga gatgtacgaa cagtggatgt aactgtacgt
     8701 cgtttgcgtg aaaagatcga agataatcca agccatccaa attatttagt gacacgtcgt
     8761 ggtgtcggtt actatttgcg taacccagaa caggagtaat tggatgaaaa gaaaagtccg
     8821 cttttttcga tcagtcaatt ttaagatcgc tattaccttt attctgattt tattgatctc
     8881 gatcgaaatt attggtgcct actttatacg tggtttagaa cgttccacga tcaatacatt
     8941 tattaaagat atgaatcaaa cggtggaatc attagcaaca acaatcagcc cggaattgaa
     9001 tcgaaaagat aatgcagatg atgaagaggt aaatgcgaat atcaaaagat ttattgaaaa
     9061 cagtgcgact agcgatatca tcgaaatacg ggtcgttgac gaaaaaggga tcatccgagg
     9121 aacaaccgat gtcaatgaac aaagcgctgt gggaaagaaa aatgattatg ttgatatcaa
     9181 tgatttcacg actaaacgtt atgttgcttt agataacgac gataaacggg tgaatatcaa
     9241 tgttcaaccg atcctgtccc cgaccggcga tgccgttatc ggggctcttt acgtcaaaag
     9301 taatatcgaa caaaaatatt cggaaatcaa taacacagca gtcattttct ttactgcctc
     9361 actaattgcg gcatttattt caatgatcgt atctgtttta gttgctcgtt cgattacaca
     9421 gccgatcggt gaaatgagag aacaagccat tcgaatcgct agaggagact acagtcggaa
     9481 agtcaaagtg catggacaag atgaacttgg acagctggct gataccttca atcagttagc
     9541 tgaacgtatt gaagaagctc aagatacaat ggaagctgaa cgcaatcgtt tggatagcgt
     9601 gctatcccat atgacagacg gcgtcattgc gacagatcgc aggggtaaag tcattacgat
     9661 caatgatatg gctgtttcct tgctagatat caaaaacgag gaagcaatcg gacaatcgat
     9721 tttgacactt ttagatatcg aagaagaata tacgttgcgg aaattattag aagatccaaa
     9781 cgaaatggta cttgaccgtt caaccagtac attggaaagt gatcagatga tcttgcgagt
     9841 agacttttct atgatccgtc gcgaatcagg ttttatcagc ggattagtag ctgtcttgca
     9901 tgatgtgaca gaacaagaaa aaacagaacg ggaacgtcgt gaattcgtct ctaatgtttc
     9961 ccatgaactt cgtacaccgt tgacaagtat gcgtagttat atcgaagcat tgagcgaagg
    10021 tgcgtggaaa gacgaggaag tagcaccaaa ttttttgaag gtaacgctag atgaaacaga
    10081 ccgaatgatt cgaatgatca atgacctctt aaatctatct cgcatggata caggtaatac
    10141 ccagcttcaa ttagaatatg tcaatttcaa cgagttagtg aattttgttc ttgaccggtt
    10201 tgatatgatg gtgggaaatc aggaaaaaaa ttataagatc cgtcgtgaat ttacgcaaag
    10261 agatctttgg gtcgaagtag atacagataa aatcatccaa gtagtcgata atatcatgaa
    10321 taacgcgatc aagtattcac cagatggggg aacgattact tgtcgcttat tagaaacgca
    10381 taataacgtg attttaagta tcaccgatca aggattgggg attcctaaaa aagatctgaa
    10441 ccgagtgttc gagcggttct atcgagtaga taaagcacgt gcaagagcac aaggtgggac
    10501 aggacttgga ttagctattt ctagagaagt gattaaggcg catcgaggcg ctatttgggc
    10561 agagagcaag gaaggaaaag ggtcgacttt ttatatctcg ctgccatatg aaccttatga
    10621 ggaggaatgg tgggaatgag aatctctgaa aaagtcgttc gtataggact cattttgatg
    10681 gttgcgttaa gcatatactt ctcttatgca atctggctga gcccagcagg aaaaagttca
    10741 atcaacgtag atgagacaaa cactcaaatg atcgagtctc aaagcaatcg aaaaacttcc
    10801 gaagtctttc ttccgcttca tgttacatgg caccattcgg gaatgatcca agaaaccaac
    10861 agtgaaaatc tcgtgtaccg attgcaattg atcatcgata aagcaagatt tggaaagctt
    10921 tctgaagtag tttcaggaga caaatcacag tttgaacagg tgaaaaatct gaactctggt
    10981 tttgagttgg cctataatgc gccttttttg ctaagagaat acaaggaagc atttgattta
    11041 gatttagatg tgtcgaactt ggcaaaagaa agcgaaaata tgtattttac tagaatacaa
    11101 ttcgatcaag gaaataaaaa aatccgattt ttgaattatt cgaaccatct cgtatatgaa
    11161 gcaaatattt cgttgaattg gacagatatc aataaagaat taacagcaaa agacacaaaa
    11221 tggacagaaa tgacaacaga accatcgacg atcgacacgc aatatgctac gaaacattcg
    11281 atcaaactga agaagtatag ttatatcctg tcacagcaac cctatacact ttttagaaat
    11341 gcttttttcc agaaaccaga tagcgtgaaa aataatgatg aaagcaatga attgattttt
    11401 tacggtggga atgaaacaat gtctatccat tcggaaacgc aagtagttga ttttcgtgga
    11461 gagctgccag agaaaaaaga aaatgctagt gtattttctg aaagtttccc ctatattagc
    11521 aaattaggca gttctttagg gaacctcaga tattttgacc gcagtgacaa tacgatcgat
    11581 tatcggatat tcgtggaagg cttcccggtt tttgggacag acagcagagg acagattggt
    11641 gtggaagttg gcggtgaaac gaatggttct gtgcaagtga atatccaaac cagtttgaat
    11701 acgattcaag tgccgatacc ttcagacgaa gaagtagaac ttgcaagtac gaatgaaatc
    11761 atccagcagt taaaagctaa gggagcagat agcaacaaga ttggttcagt gatcgttggc
    11821 tatacatggc aaaatgtcaa agaaacgaat cgtgtcgttg atctcttacc tgaatggtat
    11881 gtgaaatata aagataactg gtattcagct aacgaccttc tggaaaaatt acctggattg
    11941 gaggcgaatt aattggattt caaaaaaatc gaatggatat ttttcgtcgc ttttttaggg
    12001 ttgaatgtgt ttttattcag cagttatcat gacgcggtat accaagaaaa caatgtcatt
    12061 cgctctaatc agactgaatc aatccaacaa cgtctggcta gcgacgatat cacttacacg
    12121 gggaagattt cgtctgagaa caagcaaggc tattatctta gcgcggagca gaccaatctt
    12181 agcacttcat tagcgaatta tcgcaaggct ggtaaaagcg gcttgctggc aagtggtgtg
    12241 tttttagacg gtgatgtttt gacgaaatct ttatccagtt cagatgctga aaagaacgtg
    12301 atcgatccag ataaaattag tagttccttg gaagattttt tatctaacaa agacaatatt
    12361 ttattcggta atgattatgt gtacacgaaa aacttttctc atttagaaga agaaccacta
    12421 gaaatccaag caagccaaga gtatgaaggc atcccattca atgatgatac agcaaaaatc
    12481 gttctttctt tagaaaaaaa cgataatgat tatagcatca cgaaatacat gcagacgcat
    12541 ttatctgata tcgagcagct gcgcgaaaag acggaactgt atacggaaga ggaagcaatc
    12601 aatacattgt acatcaataa caaaatacca agaggatcaa aaattttatg gcgtcagcta
    12661 gcttattcct gtattctgaa agtccgggaa aaaaatgtgt atgttcctgt ttggcatgta
    12721 gcgatcgaaa caagcgataa agtggtccaa attgagagcg tcaacgcttt cagcaatacg
    12781 atcgtgacaa acaatactgt accaaaagtg gaagatcaat aaaaaagcga gtataatgaa
    12841 aaaagtttac aaagtaagaa gggaagtagg agtcaaatgg ctgaagaaaa accagctttt
    12901 aaaatcagta tcctagcaag tggaagttcg ggtaattcac tttatatcga aagtgaaaaa
    12961 aaacggcttt tggtcgatgc aggacttagc ggtaaaaaaa tcacttcctt gatggctcaa
    13021 attgatcgga caccggacca attagatgga atacttgtga cgcatgaaca tcgagatcat
    13081 atacatggcg tgggtgtctt ggctcgtaag tatcatttgg atgtttttgc caatcagcag
    13141 acttgggaag cgatggaacc actgattgga aatgtaccag tggaacaaaa acatctattt
    13201 gagatgggga aagtcagaac attcggcgat atcgatattg agagtttcgg cgtctcccat
    13261 gacgcagcgg ctccgcaatt ttatcgtttc cataaagaaa atcggtcatt cgtcatgttg
    13321 acggacaccg gatattgcag tgattatatg cgaggtgtca ttgaaaatgc cgatggttat
    13381 ttgatggaaa gtaatcatga tcttgagatg ttacgcatgg gaccttatcc gtggaattta
    13441 aaacagcgta ttttaggaga tagaggacat ctttccaacg aagacggtgc acttgtcatg
    13501 actgatgtga tcggtaatcg gaccaaacga atctatcttg gccatcttag caaggaaaac
    13561 aatatgaaag aacttgctca tttaacaatg gaaaatgttt tgaaagaaaa agattttggc
    13621 gttggacatg attttgaaat ctatgataca gatccagatg aagcaactga gctattttcg
    13681 atttaaacag ttcaatcaat aaaaatataa aaagcagaag ataaaatcaa tcagggttgg
    13741 ttttatcttc tatttttatt tttttagcat attaaaaaaa taaccgtatt atcgactgac
    13801 tacactgatg tgctgctttt catgtttctc atccagtatc tcatctacta atgcaatcgc
    13861 gtaatctgcc atgctgatcg tactgtcgct agcagcgttt ctttgtaaat aatcatcact
    13921 taacaaataa gtacctgtac gtttgccatg tggatcaaat aaagaagctg gactgatata
    13981 agtccaatgt agatttttag attgtttcaa ttttaacaat ccttcgtaca tattgaaagc
    14041 tgttggatag aagggtgcat ttttaggtac ttggtcaatc atacgagtcg tcttttctgt
    14101 atctaagaat aacgaagagg cacccccaac gacaattaag cgtgtatcgg ttccttcaag
    14161 catctcaatc aaatgggaca aagaagtttg atgcatttct tcttttcctt tgggagcgtt
    14221 gaaagcatca acgacaacat caaaagatgc aagatcagta gttgtcaact caaataaatc
    14281 cttttgtata aacggaacat ctgatggcaa tttttctgga tgacgcacga ttgccgtcac
    14341 gtctaaatct ctggacaatg cttcgtctaa aataaatgaa cctgcatggc cagtagctcc
    14401 aatgagtgca attttcataa aaaacctcct tttttattcc tgtccattgt aagcagttgc
    14461 tgaatattcg tcaaagaatc cgatttttga aaagctcagt gcgcattcct ttcatggttc
    14521 gtgatcattg ggtatagtta aattaagaaa actgattgat aaaaatagaa gttgaaggag
    14581 ataagaaatg aaagagtggt tagatcgtgc ctttttacca atgataacat ctgttgtgaa
    14641 tcttagaccc ttaactgctt tgcgtaatgg aatgacttac acgatcccaa taattattgt
    14701 tggctcgctg tttttcttat ttgctcattt tcctgtggat tacatcgctc agtggataga
    14761 caaaacagga ttttctatgt attttacaca agtataccag gctacatttg atgtgatagc
    14821 cttttggatc gtttttgcca ttgcatacgc ttatgcaaaa gaagaagaca tcgatcgctt
    14881 accagtcggc ataacagcct tgtctgctta tctactgctt attcagccac taaatactca
    14941 gatagatact gtctggacag gtgctgaagg gatactagga gcagttacaa ctgggctcat
    15001 catcggatgg ggctatgcac gtttagtaag acttgcgaaa gggtggaagc aaagagagaa
    15061 gatacctgaa aatgtttggc agtcttttac ttcattgatt ccaatcattg ttatagtcac
    15121 ttttacgctt atcggttcag cggtttttca acatttgttc agaatgactg ttgttcaagg
    15181 agtatggaca tatgtccagc ttcccattca atcgttcata gacacgttgt ttggtgtgct
    15241 tttattaggt ttttttgttc cgtttttatg gttatttggt cttcatggat ctgcgatggt
    15301 caatgggttg gttagtcccg tcttgcaagc gaattcatta gcaaatgcag aaatcttagc
    15361 ctcaggacaa gagttgacgg tggcaaatgg cggacatatc gttactcagc aatttctcga
    15421 tcagttcatg acggttacgg gtgctggatt gacgttaggg gctgtatttt ttatgacggt
    15481 ttttgcgaaa tcgaagaaat acagagaatt aggaaatctt gctcttttac cagccttttt
    15541 caacatcaat gaatcaatta ttttttccac accgatcgtg atgaacccga tgatggctgt
    15601 tccttttatt ttcgcaccgg tcgtttcagg attgattacc tattccgctt tatattttgg
    15661 attcgttcct ttatttacag cagtccaagt cccttggaca acaccgccga tcctctcggg
    15721 tttcttagta ggcggagtgc ctgcagcgat tttacaaggt atcgtgttgt caattagttt
    15781 tttcatttat ttcccatttt tgaaaaaaat agattctgca aactgcaaaa aggaaagaca
    15841 gacaaaaaat gatggagcag cagaaaagat gaacgactaa aaaacaaaaa tactcctgtc
    15901 gctttagatt gggaaaatca ccaatctagg atgacaggag tatttatagt catgtttata
    15961 ctagcccttt ttggacagct aattgaacaa gaacaactaa tgtgttcatt cctacgtgtg
    16021 tgatcatcga tgtccagatc cgaccagttg ctttgtactc taggctgaag aagaaaccta
    16081 ggaaaaagta gattagcaga tgaccatcat tatgagcaaa cgcaaacagc aaagagctga
    16141 tcactgctcc gacccaaaaa tttgaatatc gactgatgat ccccagtata ctgcggcgga
    16201 atacgaactc ttccatgatg ggtccagcaa cagttgtcgc caaaataaaa gcgggtgctt
    16261 ccatgatcat ctggatgata ttttgtgtat tctcggaagg ggttgctttg ccaaacaata
    16321 cagattctaa catgacagcg atgctttgca gaatcaatgc gacaaagatt ccaaccaatc
    16381 cataaataac aatctttgga gcagaagctt tttttaccgc tgattcgatc gctaagggtt
    16441 cacgttgctt aaaataaaaa atagctagga caacggcgcc tagtatatac atgactgcag
    16501 ttgctgtgat caattcagac gtagatttta caagtcctat cgaagctagg atcaagggtg
    16561 agataaaaat tagaccatag caaaaaatgc tgaggaagct atattttttt aaagacatgt
    16621 aattcctcct tttaagggat aacaagcatg caaggcaagc ttatatcgat tgatttcaat
    16681 tatatcataa tttcaataag cagctgtcag aataaaattt cttttctttg atttaaaagc
    16741 ttgcaaaaaa gagggagaat gagtattatt aaaaatgtag gttagcactc gaaggttaag
    16801 agtgctaaca gattaaaaaa agagttatcg gagggattta tcgtgttaaa accattaggt
    16861 gatcgcgtca ttatcgaagt cgcaaaagaa gaagaaaaaa ctgtcggagg aatcgtttta
    16921 gcttctgctg ctaaagagaa accccaaact gggacagttg ttgcagtcgg tgaaggccgt
    16981 ttgttagaaa acggtgaaaa agttcctgcc gctgtaaaag caggcgacca agttatgttc
    17041 gaaaaatacg ccggtacaga agtaaaatac gaaggaaaag aatatctgat cgtcgcagga
    17101 aaagatatca tggcgattgt tgagtaaatt tggaagataa aaaaataatc aaaaataatc
    17161 aatgaggtga atcaatcatg gcaaaagaat tgaaatttgc agaagacgca cgcgcagcta
    17221 tgttacgcgg ggtagataaa ttagcagata cagtgaaagt aacattagga cctaaaggcc
    17281 gaaatgtcgt actagaaaaa tcttacggct ctccattgat cacaaatgat ggtgtgacga
    17341 tcgcaaaaga aatcgaatta gaagatcatt ttgaaaacat gggtgcaaaa cttgtatcag
    17401 aagtagcatc aaaaacaaat gatattgcag gagacggtac aacgacagca actgttctga
    17461 cacaagcaat cgttcgcgaa ggtttaaaaa acgtaactgc aggtgctaat cctctaggca
    17521 ttcgtcgcgg gatcgaattg gcaacaaaag cagcagtcga agaattgcat aatatctcaa
    17581 ctgttgttga ttcaaaagaa gccattgcac aagtagcagc tgtttcttcg ggttctgata
    17641 aagttggaca tttgattgct gatgcaatgg aaaaagttgg aaacgacggt gtcatcacaa
    17701 tcgaagaatc aaaagggatc gaaacagaac tagatgttgt agaaggtatg cagtttgatc
    17761 gtggttactt atcacaatat atggtaacag ataacgacaa aatggaagct gttctagaaa
    17821 acccatatat cttgatcaca gacaaaaaaa tctctaacat ccaagatatt ctgccattgt
    17881 tagaacaaat cttgcaacaa tcacgtccat tgttgattat tgctgacgat gtagatggtg
    17941 aagcattgcc aactcttgta ttgaacaaaa tccgtggaac attcaacgta gtagctgtaa
    18001 aagctcctgg tttcggcgat cgtcgtaaag caatgcttga agatatcgct attttgacag
    18061 gtggtacagt aattactgac gatctaggtt tagaattgaa agatgcaaca atcgaaaact
    18121 taggaaatgc ttctaaagtt gttgtagata aagacaatac aacgatcgta gaaggttctg
    18181 gagaaaaaga agcaatcgaa gctcgtgtgc aattgatcaa aaaccaaatt gctgaaacaa
    18241 cttctgattt tgaccgcgaa aaattacaag aacgcttagc taaattagca ggtggtgtag
    18301 ctgttgttaa agttggtgca gctactgaaa cagaattgaa agaattaaaa ttacgcattg
    18361 aagatgcatt gaatgctaca cgtgccgcag tagaagaagg aatggtctca ggtggtggta
    18421 cagctctagt gaacgttatc agcaaagttt ctgctgtaga agcagaagga gacgtggcaa
    18481 caggtatcaa gatcgttgtt cgtgctttag aagaaccaat tcgtcaaatc gctgaaaacg
    18541 ctggttatga aggatcagtg attgttgata aattgaagaa cgtggaacta ggcactggtt
    18601 tcaatgctgc tactggagaa tgggtaaaca tggttgaagc aggtatcgta gacccaacta
    18661 aagtaacacg ttctgccttg caaaatgccg cttccgtttc tgccttgtta ttaacgacag
    18721 aagcagtcgt tgcagataaa ccagaaccag cagcgccagc agctcctgca atggatccat
    18781 caatgatggg cggtatgatg taagaaaaag caacttgctt atctacaact gatcattagt
    18841 gaaagacaga taaaaagagg ctggaacaga agcgtttaac tccaagaaat aagaagaaat
    18901 tcacggaaat tgcttttcaa atttttgtga atttcagctt atttccgaag gagttgcttc
    18961 tgcttccgct gtttatacgt gtttagagcg tgagacaaaa gtgttcttta cttttgtctc
    19021 acgctctttt ttgtgtgtaa ttataatgaa aatagttatt tttaatagtt ataatatttt
    19081 atctattaaa agta
//
LOCUS       DAZKKW010000052        18504 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.052, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000052 DAZKKW010000000
VERSION     DAZKKW010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18504
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.052"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            99..746
                     /locus_tag="ACG8PO_001750"
     CDS             99..746
                     /locus_tag="ACG8PO_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HFQ0207059.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     misc_binding    875..1091
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1139..3784
                     /locus_tag="ACG8PO_001751"
     CDS             1139..3784
                     /locus_tag="ACG8PO_001751"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFQ0207060.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            4094..5407
                     /locus_tag="ACG8PO_001752"
     CDS             4094..5407
                     /locus_tag="ACG8PO_001752"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HFQ0207061.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            5397..6050
                     /locus_tag="ACG8PO_001753"
     CDS             5397..6050
                     /locus_tag="ACG8PO_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFQ0207062.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            6105..6797
                     /gene="radC"
                     /locus_tag="ACG8PO_001754"
     CDS             6105..6797
                     /gene="radC"
                     /locus_tag="ACG8PO_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFQ0207063.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGNVIPSQQDMNFTKRIQKCGEMMGITVLDHLIIGRKRY
                     FSLREEGMMEEK"
     gene            6947..7147
                     /locus_tag="ACG8PO_001755"
     CDS             6947..7147
                     /locus_tag="ACG8PO_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFQ0207064.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(7401..9080)
                     /locus_tag="ACG8PO_001756"
     CDS             complement(7401..9080)
                     /locus_tag="ACG8PO_001756"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HFQ0207065.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(9082..9294)
                     /locus_tag="ACG8PO_001757"
     CDS             complement(9082..9294)
                     /locus_tag="ACG8PO_001757"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit epsilon"
                     /protein_id="HFQ0207066.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            9687..11417
                     /gene="efrA"
                     /locus_tag="ACG8PO_001758"
     CDS             9687..11417
                     /gene="efrA"
                     /locus_tag="ACG8PO_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HFQ0207067.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            11414..13174
                     /gene="efrB"
                     /locus_tag="ACG8PO_001759"
     CDS             11414..13174
                     /gene="efrB"
                     /locus_tag="ACG8PO_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HFQ0207068.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            13269..13466
                     /locus_tag="ACG8PO_001760"
     CDS             13269..13466
                     /locus_tag="ACG8PO_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0207069.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            13619..14104
                     /locus_tag="ACG8PO_001761"
     CDS             13619..14104
                     /locus_tag="ACG8PO_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFQ0207070.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(14208..14801)
                     /gene="clpP"
                     /locus_tag="ACG8PO_001762"
     CDS             complement(14208..14801)
                     /gene="clpP"
                     /locus_tag="ACG8PO_001762"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFQ0207071.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            14981..15502
                     /locus_tag="ACG8PO_001763"
     CDS             14981..15502
                     /locus_tag="ACG8PO_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0207072.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDV"
     gene            15599..16336
                     /locus_tag="ACG8PO_001764"
     CDS             15599..16336
                     /locus_tag="ACG8PO_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFQ0207073.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            16340..17257
                     /gene="miaA"
                     /locus_tag="ACG8PO_001765"
     CDS             16340..17257
                     /gene="miaA"
                     /locus_tag="ACG8PO_001765"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFQ0207074.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            17259..18488
                     /gene="hflX"
                     /locus_tag="ACG8PO_001766"
     CDS             17259..18488
                     /gene="hflX"
                     /locus_tag="ACG8PO_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HFQ0207075.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEIAVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
BASE COUNT         5984 a         3200 c         4022 g         5298 t
ORIGIN      
        1 atttgtctca cacccttttc cttcctactt gtaaccttgc tcccgtgtta gaaacatgtt
       61 acactaaata tgtgaaatta ttaacaagga gcgtcgttat gcgagaaaaa acagatacac
      121 tgccaaaagc aactgcgaaa agattacctt tatatttacg ctacttgaag atgctggatg
      181 actcaggtat aagtagaatc aaatcaaatg agttcagcga aatcacccaa attccttctg
      241 caacgatccg cagagacttc tcgcaattag gcgagctagg cagaagcgga tatggctatg
      301 atgtcccttt tcttatcgat gtattcaata atatccttaa tactaaagaa gaaaaacgaa
      361 tcgcattagt aggatatgga aatcttggca aagcattgaa acacaacaat tttagaagaa
      421 atgaaaatct aaatatcgtg tgcgtctttg ataatgatcc agcactgatc aatcgagtaa
      481 ttgatggaga aatgatctat ccgattgatc gtttcgcgga gatagccaaa gcaaaaaatg
      541 tgacagtagc aatctctacg gtaccaagta aatattcaca atctgctatt gatgaaattg
      601 tcaaaggaaa tgtaaccgct atacttaatt ttgcgcctga cagagtaact gtcccagctt
      661 atgtcaatgt tcaatacatt gatttgacaa ccgagttgca gacgcttatt tactttgatg
      721 agaattactc agaagttttt tcttaggaaa atttgagtag gcttattgat cttgcgagaa
      781 ctcgtcgtaa ttctttcaaa aattttttag tactaaatga agtggttttt tcacgaccac
      841 ttgttgacta aaaaaaaaaa ccattgtaga atgaagcata acgctgaaca agaggagtaa
      901 ctggcacatt cattcttaga gagagaacta tctgctgaaa ggttctcaat gacaaccagt
      961 gaaggtagct tgggagtttc tgtcttgaat ggagtagaga tagacgagtc gtgatcgtta
     1021 actcacttga gaggcaataa tgattgatta ttgcaattta ggtggtaccg cgtaaatcat
     1081 acgtcctaga atacgaaagt attttaggac gttttttagt ttaaaggagg aaaacagcat
     1141 gacggaagaa aataacctat caacaaaata taatccgcaa gaagttgaag ctggacgtta
     1201 tcagcattgg ttagaacaag atttgtttaa accgagcgga gataaaaaag caaaacctta
     1261 ttcaatcgta atcccgcccc caaatgttac tggaaaactt catttagggc atgcttggga
     1321 tacgaccttg caagatatgc tgattcgtca aaagagaatg caaggatatg atactttatg
     1381 gctgcctggt atggaccatg caggaatcgc gacacaagca aaagtagaag cgaagcttag
     1441 agagcaaggg atttctcgtt atgatctagg tcgtgaaaag ttcatcgatc aagtgtggga
     1501 atggaaagaa gagtatgcga gccacatccg cgaacagtgg gctaaaatgg gcttatcttt
     1561 agattacagt agggaacgtt ttacattaga tgatggatta tctgaagcag tacgtaaagt
     1621 ttttgttacc ttatacgaaa aaggtttgat ttatcgtggt gaatatatca tcaactggga
     1681 tcctcaagca cgaacagctc tatctgatat cgaagttatt cataaagata tcgaaggcgc
     1741 cttttatcat atgagctatg aactagcaga tggatcaggc gtagtagaaa ttgcgacaac
     1801 ccgtccagaa acgatgttgg gagatacggc gattgctgtt catccagaag acgagcgtta
     1861 tcaagcgctc atcggcaaaa aagtgattct acctttagtc aataaacaaa tcccaatcat
     1921 tgcagatgaa tatgtagata tggaattcgg gacaggtgta gtaaaaatca ctccagcaca
     1981 tgatccaaac gactttgagg taggtaatcg tcatgatctt ccacgcgtaa atgttatgga
     2041 tgatgatggt acgatgaatg aactagcagg aaaatatgct ggaatggaac gttttgctgc
     2101 tcgtaaagca atcgtgcaag atctgaaaga aatcggtcga ttgattaaaa tcgatacgat
     2161 gatccacagt gtgggtcatt ccgagcggac aggtgtcgtt gttgaacctc ggctgtctac
     2221 tcaatggttt gtaaaaatgg cgccgttagc gaaaaaagcc attgataacc aagaaacaga
     2281 tcaagcagtg gaattcttcc ctccaagatt caatcaaacg tttttacgtt ggatggaaaa
     2341 tgtccatgat tgggtaatct cacgacaatt atggtgggga catcaaattc ctgcatggta
     2401 tcacaaagaa acaggtgaaa tgtatgtagg gatggaagca ccagcggata gtgaaaactg
     2461 ggtacaagat cctgatgttt tagacacttg gtttagttca gctttgtggc ctttctcaac
     2521 aatgggctgg ccagatacgg aaagcgaaga ttataagcga tacttcccta ctagcacttt
     2581 agtaaccggt tatgacatca ttttcttctg ggtaagcaga atgattttcc aaagcttgga
     2641 atttactggc gaacgtccat ttgagaatgt cttgattcat ggattgattc gtgacgaaca
     2701 agggcggaag atgagtaaat cattaggtaa tggaatcgat ccgatggaag taatcgaaaa
     2761 gtatggagca gatgctctcc gctggttctt atcgaatggt tctgcaccag gacaagatgt
     2821 ccgcttcagc tatgaaaaaa tggatgcttc ttggaacttt atcaacaaaa tctggaatgc
     2881 ttcacgtttc gttatcatga atatcgaagg aatgaccgta gaagagatcg atttatctgg
     2941 agaaaaatca gtggctgacc ggtggatcct tactcgtttg aatgaaacga tcgaacgagt
     3001 aactgagcta tttgatcgtt ttgaatttgg tgaagcaggt cgacaactgt ataactttat
     3061 ctgggatgat ttttgtgact ggtacatcga aatgagtaaa gaagtacttt atggagatga
     3121 tgctgtttca aaacaaacca cccgtagcat cttagtccat acacttgatc agatcctacg
     3181 tttattgcat ccaatcatgc cattcgttac agaagaaata tgggaacata ttcctcatca
     3241 gggaaattct ctagtagtgg cagaatatcc tgttgtacgt ccagagtttg atgatgaaac
     3301 agcttctaaa gggatggaag tattgaaaga attgattcga gccgttcgaa atattcgttc
     3361 agaagtgaat acaccattgt ctaaaccaat tacgttgcta attaaaacaa acgatgctaa
     3421 aatcgatcaa tttttagttg aaaatacaag ctatatcgaa cgcttctgta atcccgaaga
     3481 gttgacgata tcaagtgaga tcactgcgcc agatctagcg atgtcagctg ttctgacagg
     3541 tgcagaaatc ttcttgccgt tagcaggatt gatcaatatt gaagaagaaa tcaaacgatt
     3601 agaaaaagaa ctggaaaagt ggacaagcga agtaaaacgt gtccaaggaa aactttctaa
     3661 tgaacgtttt gtttctaacg cgccggaaga agtagtagag gcagagcgtg caaaagaaaa
     3721 agattattta gagaagcaaa cggctgtcaa agaacgaatc gaaagtttgc gtactattca
     3781 ataagaatag aaaaagagag tgtgaaatca gctagctatt actaaaagct agctgattga
     3841 acatcgttac aggaaagagt ttgagcctca ctcttagctt cgattcataa ggagatgtga
     3901 cataaatttt gtcgcatctc ttttttagtc tactcttatt atgtaggaga aggagggagg
     3961 tggatcatgt atcaagagtc aataaagtag ttgataaaat cgttcctctc tctgtttctt
     4021 ttctctttgt catactttta tttttcttat gtgttctatt tgttataata aaagagaaat
     4081 gggggaattg ttgatgacga ttgaagaagc aatagaatgg atccatagtc gattaccatt
     4141 tggttcacgt cctggccttg accgagtcga agctttatta gaagtggtag ggcatccaga
     4201 gagaaaagta aaaacgatcc atatcgctgg aacaaacgga aaaggttcta ctgtagctta
     4261 tttgagaagc ctattagaag aggctgggct gacagtaggt acatttactt caccttatat
     4321 cgaaacgttc aatgaacgga tcgcaatcaa tggcaaaagc attccagaca atgatttgat
     4381 ccagcttgta aaaaaatatc agccactgat caaacaactt gatcaatttg aagcagtagc
     4441 tggtattact gaattcgaga cattaacagc aatggcattt gattattttt tgatgaagaa
     4501 agtggacgta gcgattatcg aagttggctt aggcggctta ttggattcta ccaatgtagc
     4561 tgtaccaatg ctgacgggaa taacaacgat tggacttgat cataccgaaa tacttggaga
     4621 tacaattgaa gaaatcgctg cacaaaaagc tggaatcatc aaacaaggcg ttccagtagt
     4681 aacaggaaat attgccccag aagcttttac tgtcatccaa caaacagcta aagcaaaaaa
     4741 tgcaccgctt tatgtatacg gggaaaatta taaaataaac tatctgcatc ctgatgagat
     4801 gtggggagaa gtatttgaat tttttaatga aataggaaag atccctcaat taaccactcc
     4861 gcttttaggg caacatcaag ctgaaaatgc aggagtagcc atcgaattgt attttgttta
     4921 ctgtcaattg gaaaagctgc ctttccgaga aaaagatgtt ttgaagggat tgaagaaatc
     4981 tcgatggcca ggaagaatgg agcgtttgag taacgagcca ttagttgttt tagatggtgc
     5041 ccataatacg catgcaatga accggcttgt tgagaatttg aaaaaagaat tcaaaggata
     5101 tcaaatcaac attttgtttt ccgccttacg aactaaaaat gttagagaaa tgctagaacg
     5161 attgcagcaa gtaccaaatg tccatattta tttgacgacc tttgaacatc caaaagccat
     5221 cgaattaagt gaagtatcag atttagcaag tgagagagta tcaatcgttt cattgtggca
     5281 attcggatta gcagatatat tggaaaagat gacaaatgaa gacgtattag ttgtgaccgg
     5341 ttcactttat ttcatttcag aagttcgcca tttattaata caattaggag gagatcatga
     5401 agtataatgc agtgattttt gacatggatg gtttgctgtt cgacacagag ttagtgtatt
     5461 acgaagccac acaagtagta gcagacaaga tgggttttcc ttatgataaa gacctgtatt
     5521 taaaattctt aggtgtttca gatgaagaag tatgggcgaa ttatcatatg attttctcgg
     5581 aatttggcaa aacaagtgta caaagattca tagatgattc ataccaggaa acgcttgaac
     5641 gtttttcgca aggagcggta caaatgaagc ccggtgtgtt ggaactcctc tcttttttag
     5701 aagaacatca gattcccaaa gttgtcgctt ccagtaacca acgagcagtc attgaacttt
     5761 tattgaagaa gaacaatctg tttgaacgat ttgagacgat tgtttctgca gaaaatgttc
     5821 aacgagcaaa accagaccca gaaattttct tgttagctaa tcagaagcta ggtacagcga
     5881 agaaagaaac attgatttta gaagattcac aaaatggtat tttagcagct tctgcggctg
     5941 agatcccggt agtcatggta ccagatctgc ttccaccatc ccaagaattg atcaaaaaaa
     6001 cagctggggt tgtttcttct ttacatgaaa tccctgcatt gttaaaataa aaatccatct
     6061 cttttcgtat cttattttag ataagataga aaggagatgt ttttttggga aaacgattga
     6121 tcaaagaagt acctactagc tctttaccaa gggaacgtat ggaaatttat ggcgcggaag
     6181 ctttatcgga tcaggagctt ttggcaatct tactgcgtac cggtcaacac ccttatagtg
     6241 tgatgtcgat tgctggaaat ctgttaaaaa cgtttggagg actggcctct ttacgacagg
     6301 ctactttgca tgagttagaa gagattcgag ggataggacg cgtgaaagca atcgaaatca
     6361 aagccttgat cgaattaggt cgacgaattc agacggatca tgaaagagcg gctccaccgg
     6421 ttcgtaccag ctacgaattg gcccagcaat tgattctgga aatgaaagat cacaaacaag
     6481 aacatttggt ttgtatttat ttggacacga agaatcaagt gatcttaaaa aaaacagtgt
     6541 tcatcggttc gctgaatcag tcagtcgctc atcctagaga aattttccat tatgctgtac
     6601 gttattgtgc agcaaggatc gttttagcgc ataatcatcc ttctggtaat gttattccat
     6661 cgcagcagga tatgaatttt accaaaagga tacaaaagtg tggagaaatg atggggatca
     6721 ctgtactgga ccacttgatc atcggacgca aacgatattt tagtttgaga gaagaaggaa
     6781 tgatggaaga gaagtaagtt gctttcttgc aaaaaaaatg aagcaagtgt acaattatat
     6841 tgtaattttg tttgggaata gaagttgaaa catctgttgc acctctatcg tacacgagca
     6901 atgttacaat ttaaattagt gcttttggca cgaggaggat attattatgg aaaacggaac
     6961 agtaaaatgg ttcaacgctg aaaaaggatt tggatttatc tcacgtgaag atggaagcga
     7021 cgtattcgtt cacttctcag ctatccaagg tgacggattc aaaacattag aagaaggtca
     7081 agcagtgact ttcgacgttg aagaatcaga ccgcggccca caagctgcaa acgttgtaaa
     7141 agcttaattt tatctgtaaa tcaaattgat ggtagacatc aatttttacg agagaacaaa
     7201 gcgagtaaca aaaatacctg ccacaagaga caatttctct tgtggcaggt atttttttgt
     7261 atgaaaaagt aaataaattt aagaggctat gaaagagcca ccttgtatca aggaataaga
     7321 acaaacaaaa atggcttctc atgtttttca tttgttttct cttatattca ggacaaggtg
     7381 ctgatttcat aacctcttat ttattcttca tctggtgtca ttaccattgg caaaatcatt
     7441 ggatgtcttt ctgtacgttc gaataagaaa ggctgaagcg cgccagtcat cgcttctttc
     7501 aatttatatt ctgaacagtc aggtccattc aaacattctc ttaaagcgtg gaataagatt
     7561 cgctgagctt cattgatcat ttctcctgat tctcgcatgt agacaaatcc acgagagaga
     7621 atatctggac cagccaagat ttcttttttc ttgatatcca ctgtcgcgac agctaataca
     7681 agaccttctt ctgaaagcag gtgacggtca cgtaaaacga cattaccaat atcaccgatc
     7741 ccgctaccat cgatatacac atctccagcc acaaaatgcc cagcaggacg cgcagaatcg
     7801 ctagttaacg ctaaaacatc gccattttcc atgatgaagc agttttctgc aggaacacct
     7861 acatcttgtg ctagagaagc atgtactttc aacatgcgga attctccgtg gacaggaata
     7921 aaatattttg gcttcatcaa acgaagcatt aatttttgtt cttcttgtcc accgtgaccg
     7981 gaagtatgga tgttattgat ttttccgtga atcacttctg caccagcttc agacagcaaa
     8041 ttgatcaaat gattgacact tgttgtatta ccaggaatag gtgaacttga aaagatcact
     8101 gtatcgtctg gctggatttg gatttgacgg tgagtaccat tcgcgatgcg gctcaacgcg
     8161 gccatcgctt ctccttgaga acctgtacat aggatcatcg tttcattggc aggcaagcga
     8221 ttgatttcgt gtgcatctac aaatgtgccg tctggaactt tgatatatcc taaacgttga
     8281 ccgttgacga tggcattttc catgcttcgt ccgaaaacag cgattttacg accagttgcc
     8341 acagcggcat ctgctgcttg ctgcaaacgg aagatgttag aagcaaagct ggcaaaaatg
     8401 atacgtcctt cgatttttga aaagatcttc aaaatagaat gcccaatcgt tttttctgat
     8461 ttagtaaatg ttggaatctc agcatttgta ctatcagata aaaggcacaa gactccttct
     8521 tccccgatac gagccatttt atgcaagttt gctggttcgc caacaggagt gaaatcgaat
     8581 ttgaagtctc cagtcgctac gatatttcca gaaggtgttt tcaccacgat ccctaatgca
     8641 tctgggatac tatgggtcgt tctgaaaaag ctgacagaag tctttctaaa acggatgatc
     8701 gtatcttcat tgatctcatg gagctctgca tctcgaagca agccatgctc atctaatttg
     8761 tttgtgatca acgctaatgc gagaggacca gcatagatag gaatatttgc ttgtttcagc
     8821 aagtagggta ttccgccgat gtggtcttcg tgcccgtgag taatcaccaa ggcttttact
     8881 ttgtgtaagt tttgtatgat gtagctgtag tcgggaatga catagtcaat tccaagtaaa
     8941 tcgtcttccg ggaatttgat cccggcatca atcaaaatga tttcatcttg gaattgtacc
     9001 ccatacgtat ttttcccgat ttcacctaat ccaccaatag caaagacgcc tgtctcgtta
     9061 tttttgatgg aaagtttcat attaaaactc cactaatgag aaatcagcat tttctttttc
     9121 gtactctaga tgacttcctt ctagaagttg aacgaattca atattgtatg gggtattttc
     9181 ttccacacgt tggcgagcgt ctacatcgct ttttgcctca atgtaaagag attttgtttc
     9241 ttcccgttta gggttgcgta cttttgtttc ttgataataa actttataga tcattgtttt
     9301 gtctccttta tctgcgtaaa gcagtgattt agctagttga tttttttaga caacacaaaa
     9361 cgaggggaca agccacctgg tttttcatat gctttgacta gaatactgga ttcgagtcaa
     9421 agagacaagc ctcgtttttg cttatctgtt cggatatttt ttcaaccgta ccttctgtat
     9481 aaaaaatata caacaattaa ttgttattct agcataaaac agcaaataag tatagaaatc
     9541 aactagattg aaagaaaaaa tcaatgttta tcatcccgct ataatcataa tgactttttt
     9601 atgagagaag tgttctatta ataggctcgt tgtgctatca tttttagtaa tgaaaatgta
     9661 acaaaagatt aaaaggagat gagaaagtga aactaatgtg gcattatacc atgcgttata
     9721 agaaattctt gtttttcaat ttcatatgcg tatttggttt tattttgatt gaattaggac
     9781 ttcctacgat tttagcacgt atgatcgatg taggaatccg caatgatgat tttgattatg
     9841 tgaagcaaca agggatattt atgatcgtta ttactgtaat cggtattatt ttgaatatct
     9901 tgttaggtta ttttggttcg aggattacga ctaatatcgt agcagatatt cgagatgatt
     9961 tgttcgaaaa gatccaaagt tattcgcatc aggaatacga aaatctaggc gtatcttcat
    10021 tgatcactcg tacaacaaac gatgcttatc agatcatgtt gttcctgcaa aatattttac
    10081 gtatcggttt tatggcacca atgatgttca ttgtcagttt gtacatggtc atgcgtacaa
    10141 gtgtgacact cagtttgtat gtagtaggtg cattaccttt actattattg gcagtcgtag
    10201 cgattgctaa ggtgtctgaa ccgttatcta aaaagcaaca aaaaaactta gataaaatca
    10261 acagtatctt gcgggaaaat ctttcaggat tgcgtgtgat tcgtgcattc gtcaatgaaa
    10321 agtttgaaga aaaacgtttt gctgtagtca atgagaatta tgcttctagt tcaaaaagct
    10381 tattccgttt gatggctgtt gcacaaccgg gtttttattt cttatttaat atcgtaatgg
    10441 tattgatcat ctggagcggg gcagtgcaaa tcgatgcagg cgagcttcgt gtaggagatt
    10501 tgattgcgtt tatcgagtat atattccatg ccttgttctc attcatgtta tttgctagtg
    10561 tgttcatgat gtatccaaga gcagctgttt cagctggacg tatccaagaa gccctagatg
    10621 ccaaaccttc gattgtagaa gatccaaatg gtatatctga tacaaaaact aaagggtata
    10681 tcgaatttca aaatgtaaca tttgcttatc caggccattc gcaagagcca gtgatacgaa
    10741 atgtcagttt tacagcctct ccaggagaga ccgttgcttt tattggaagt accggtagcg
    10801 gaaaatctac gttgatccaa ttgatccctc gcttttatga tgtgagtgaa ggaaagatct
    10861 tgatcgatgg agtagacgtg agagattatc ggctaagcaa gttgcgagat aagattgggt
    10921 ttattccgca aaaagcgctg ctatttactg gaacgattgc agataatttg cgatacggga
    10981 aagaagatgc tacgcaagaa gaaatggaac gtgcagctga gattgctcaa gcttcagaat
    11041 tcatttctcg aaaaccagat ggatttaatg aacatttatc agaaggtggt tccaattttt
    11101 caggtggaca aaaacaacga ttggcaatag ccagagccat catacgccgt ccggaaatct
    11161 atatttttga tgacagcttt tctgcattag attatcaaac ggatgcgaag ttacgtgctc
    11221 gtctgaaaaa agaaacgaca gaatctactg tattgatcgt agcacaacgt gtgggaacga
    11281 tcatgcatgc agataagatc atcgtattaa atgaaggaga agtcgtcggt atggggacgc
    11341 atcgggagtt gttggaaaaa tgcccgattt attatgatat tgccgcttct caattatcaa
    11401 aggaggaatt agcatgaaaa aaaccttttc ttccttaaaa cgtttagctc gatatatcag
    11461 accgtatcgt gagacttttc ttttagtgat cctatttaca gttctgacag tggctttcaa
    11521 cgtggctttg ccatatgtta caggacttcc tactacagaa atcagccgta atattgccaa
    11581 tggcgaatca atcaattttt cttatatatt caaatgtctc atttggatca cagttgttgg
    11641 aatagggtat tgtatttcgc aattactttc tggtgttttg atgacaaatg tcgttcaaag
    11701 tgccatgcat gatttgagaa gagacataga agaaaaaatc aatcgtcttc cggtttcata
    11761 tttcgataaa aaccagcaag gaaatatttt gtctcgtgtg acgaatgatg tagatgcagt
    11821 cagcggtgct ttacaacaag cttttattgg tatcgtaaat gcgatattgg gaattacgtt
    11881 ggctgcagca atgatgtttt atatccagcc gatgatggcg ctgatctcta tgatcatgat
    11941 tccaacatcg atctggatct caaaacgagt gatcaatgct tcgcagaaat attttcaaag
    12001 catgcagaat tctcttggtg agctaaacgg ctacgtccaa gaaaatatga caggttttag
    12061 tgtattgaaa gtatatggac gcgaaaaaga aacattggaa ggattcaaaa aagttaatca
    12121 cagcttgaaa tactttgggt ttcgtgcagc attcatttct ggcttgatga tgcctctagt
    12181 tcaattaact gcctatgcta cttatattgg tatggctgtt ctaggaagct tttatgttat
    12241 tactggggcg atcgttgtcg gtcaactaca agcattcatc caatatattt ggcaaatcag
    12301 tcagccaatg gggaacgtga cacaattgtc ttcagttttg caaagtgcat cagcggcaac
    12361 gaaacgtgta ttcgagattt tagatgagcc agaagaaaaa gaaaacaaag tagatgcttc
    12421 actgccagaa acgattttag gagatgtgac attcgatcat gtcgattttg catatgatcc
    12481 gaagaaacca ttaatccaag atttgaattt tgaagtgaaa gctggtcaaa ccgtagctgt
    12541 ggtcggtccg accggtgccg gcaagacgac attgatcaat ctattgatgc gtttttatga
    12601 cgtagacaaa ggagcaataa ggattgatgg aatcgacacc aaatcaatga gtcgcagtga
    12661 tgtgcgttcg ctatttggta tggtcttgca agatgcatgg ctttatcaag gaacgattgc
    12721 agataatatc cgcttcggaa agcttgatgc gacagattat gaagtagttg atgctgcgaa
    12781 aacagcaaac gtagatcatt ttatccgaac gatgccagat ggatatgata tggccatcaa
    12841 ttctgaggga gacaatgtgt ctttaggaca aaaacagttg ctgacgattg cccgagcagt
    12901 ggtctccgat cctaaaatct tgattttaga tgaagcaaca agttcagtag acacccgttt
    12961 agaagcgttg attcaaaaag caatggatcg cgtgatggag ggacggacaa gtttcgtcat
    13021 cgctcaccgt ttatcgacaa tccgtgatgc agatctgatt ctagtgatgg atcaaggtca
    13081 gatcattgaa aaaggaacgc atgagagttt attggcacaa ggtggatttt atgaaaaact
    13141 gtataacagc caatttgctg aggaagcaga ataagtcata taagaataaa acagtttaaa
    13201 acttattttt tgttctctat tcttgataat ggtaaagtaa agttatcaag aaaagtgagg
    13261 acacggagat ggaacagttt ttaaatgaaa aaattactga agccaaaaga gaagttgatc
    13321 gtttaacgca cgtctgtcag acgaagcctt ttgattcagc tgattttttt gaaaatgagt
    13381 ttcaaatcga acgcttggca gcacaaatag aagtgtacga attaatttta aaaaaagtaa
    13441 tagaagaaaa tatagatgta caataaaaac aggtaaaacg tcagttacca gatactgtaa
    13501 atagacatct ggcctgagca ggttttacct gttttttttc ttatgaaata aatatatgct
    13561 atgatacctt tattcaatag gattagtttc tacaaaaaat gaaaggagaa aaataaatat
    13621 ggaacggttt atagcatcaa atgccacagt gattggtgat gtgacattgt cagaagatgt
    13681 gacgatctgg tatcaagctg ttttacgcgg ggacagtaat tggataaaaa taggacaaag
    13741 aacgaatatc caagatggta caatcatcca tgtcgatcat gatgcacctg tagatattgc
    13801 ggagaatgtg acggtaggac atcagtgcat gctacatggc tgtacaatcg aaaaaggggc
    13861 gctgatcggg atggggacca cgatattgaa tcatgcggtt attggtgaga atagtttgat
    13921 tggtgctgga tcattagtaa cagaaggaaa agttattcct cctaatgtct tggcttttgg
    13981 tcgcccagct aaagtgatcc gtcctttgac aaaagaagaa atacaaaaaa acaaagaaaa
    14041 catccagcat tatgtcgaga taggtcggat gcatgcgcaa ggaaaattca aggagcaaag
    14101 ataggcagta gcatccataa aataaaaaag gatggtcagt ttgagttgac catccttttt
    14161 tatttggttt agataagaga gcttaaaaaa gcttcctttg aaacagatta atttaatgaa
    14221 gaactgtttt ccatgacttc atcgatcaat ccatatgctt ttgcttgttc agctgtcata
    14281 tagttgtcac gatctgtatc tttttcaatc acttcaagcg gttgtccagt gcgttcagct
    14341 aaaattttgt ttaagcgttc gcgtgtttgc aaaatatggc gtgccgcaat ttcgatttca
    14401 gttgcttgcc cttgagcgcc gccaagaggt tgatggatca tgatctcagc atttggtaaa
    14461 gcaaatcgtt taccttttgt accagctgtc agcaagaagc ttcccattga agcagccata
    14521 cccataacaa ttgtttggac atctgctttg acaaagttca ttgtgtcgta aatagccata
    14581 cctgctgtaa ctgaaccacc aggagaattg atataaagat agatatcttt ttcagaatct
    14641 tgagcgtcta agaaaagcaa ttgggcgatg atcgaattag caagatcatc tgttacttgg
    14701 ccgcttaaca tgatgatacg gtctttcaat aaacgtgagt agatatcgta agctctttca
    14761 cctcgtgaag attgttcaat gactgtagga attaaattca taatagagtt cctccttgat
    14821 ttcgtttaga aatagtgtac cataatttag tttctaatac ctaaaatgta gtatacattt
    14881 ttggtcaaaa aaggtcaaat aaaaaactta ggcaaattag tcgcataaac ccgctttatg
    14941 gtatgataag cagagaaaca aaacaaggag gaacaacaga ttgatagttg gtattactgg
    15001 ccttatcgga gtgctgacag gtgctgtagt tgtctatatc ggcatgatac tgcaaatgcg
    15061 tcacttggaa aataaagaac agcgtagacg tgaattggaa ttgaaagtca aagaaatcga
    15121 gacgctgaat caattaaata aaaagatcaa cgagatcttg caaaaacgga caacgctgat
    15181 ggaagaatat gtcagctttg atgcatttga tgattgttat atcacgattg acgattttgc
    15241 gtatctccaa tcattttcag cgcaaaatag tttttatctg ccaaattata ttttagacga
    15301 gctatttaag aatatctccc atcgaaaagt gattttatct ccagatgaaa caatgaagat
    15361 tggtggatat gcttataaag gcggacgcgt gattttagag aattttacgg atagtctgac
    15421 tgaaatgatc aatgaaaaaa aagctcagtt gaagactatg acgcgccagc cgattggttt
    15481 cttctcgaaa gatgatgttt gaagctgata ttttgaagca ttgccgttgc acatgagagc
    15541 gtggtaatgc tttctttttt tcttaatgat acaatagaac tgaacggagt gaagaaagat
    15601 gttacaaatc tatgcccatc gcggaagcag cggcacccac ccagaaaata cgcttcctgc
    15661 atttgcagaa gctgtacgag taggtgcaga tggtatcgaa ctagatgtcc atctaagcaa
    15721 agatggttat ttgatcgtta tgcacgacga agaagtggac cggacgacta acggtaaagg
    15781 gctgattcga gaaaaaacat tggaagaact caaaaaattg aatgcagggg gcaaattcag
    15841 caaagaattt ccagcagcta aagttcctga attgagagaa gtgttaggac tgctcctcca
    15901 aaaaaattac cgagggatgc taatgattga aattaaaact gatcagtatg aatatcccgg
    15961 tatcgaagaa aaattagccg aaacattaaa aagtagagat tggccttttc aacatgcata
    16021 ttgcagcttt catttggatt ctttggaaaa aatcgcatta ctggaaccgg agatacagct
    16081 ggacttattg atgagtactt ctgataaaaa gatccaacta gcagcagaaa ccccttatat
    16141 agaaggagta catccaaaaa atacttggtt gttcgaacat ccagtccagg cagctgattt
    16201 tccaaaagca attcgtgcat ggacgattaa cgatgaagaa gaaatggcca aatgtcaaga
    16261 actcaaaata gatggaataa taactgattt tccggagaaa gctcttcggt ggaaacaatc
    16321 aacgaaaagt aggtaaaata tggaaaaagt attagtgatc gtcggtccga cagcagtcgg
    16381 taaaacagcg ctaagtgtgg aactcgcaca actatttgga ggagagatca tcagtggtga
    16441 ttcattgcaa atctataaaa atctggatat cggtacagca aaaatcaaag aagaagagat
    16501 gaataatgtt ccgcatcatt tgattgatgt gatcgaacct actcaacgtt actcagccgc
    16561 tgattttcag cattcaggtc gacaattgat taccgagatt gcgaaccgag ggcacctacc
    16621 tatcatagct ggtggtacgg gtctctatat ccaatccctt ctatatgatt atcagttagg
    16681 agcgacagaa gaagcgaatc ctgagattag aaaaaagtat gaaacgttag ctgaaacaat
    16741 tggttcccaa gcattatgga atcagttgaa agaaaaagac ccggcagctg ctgagaaaat
    16801 ccactggaac aatcaacgca aagtgataag agctctggaa gttttagaga cgacaggaca
    16861 tagtatcacg gcacctaaag aagagccaaa gcaattgtat gattacctca tgattggatt
    16921 agatacgaag cgtagtattc tttatgaacg aatcaatcaa agagtcgatt tgatgctagc
    16981 agaaggttta gtagcagaag cgcgtaaggt gtatgagctt ggagaagtcc aggccagtca
    17041 aggtattgga tataaagaat ttttccctta ttttgaaggg aaagaaacat tggaagaagc
    17101 aacagaacaa gtcaaactac attcgagaag atacgcgaaa cgtcagttga cgtggtttcg
    17161 taaccgtttg gatgcacagt ggtttgatct tataagtgag ccaaatcaaa aaagtcagat
    17221 tgagcaaaca atcaaaaaat ggctggaggc ggaataaaat ggaagaaaaa gtgatcatcg
    17281 taggggtaga aaccgaagca aaccagcgtt acttttcaga gtcaatggaa gagctagttc
    17341 agctgacgaa taccgcatca ggagaagtcg tattcacaat tacacaaaag agaccacaag
    17401 tggatcggca aacgattatc ggtaaaggga aattacaaga attagttcaa caagcggatg
    17461 cgcatgaagc ggatttgatc atctttaatc atgagctaac tccgagacag agccaactga
    17521 ttaccgatgc tgttggtctt ccggtaattg atcgcgtgca gcttatctta gatatttttg
    17581 cgatgcgtgc tcgttcgaaa gaagggaagc tgcaggttga acttgctcag ttagaatacc
    17641 tgctgcctcg tttagtaggg caagggaaaa cattatctcg tcttggcggt gggatcggaa
    17701 caagagggcc aggtgaaaca aagttagaaa cagaccgccg acatatacgg aataaaattt
    17761 ttgctgttaa gaaagaattg aaagaagtag aagctcatcg agagcgcaat cgccaaaaaa
    17821 gaaaaaatag cgagattttt cagattggac ttattggcta cacgaatgcc gggaagtcaa
    17881 ctatcttgaa tctattgaca caagcggata cttattcgaa agatcaattg tttgcaacgt
    17941 tagatccatt gactaagaga tggcgttttg ctgaaggatt tgagattgct gtaacggata
    18001 ccgtcgggtt catccaagac ctgcctacgc aactgatcga tgctttccat tctacattag
    18061 aagaaagtca aaatatggat cttttgctac atgtagtcga tgctagttct ccagatcgta
    18121 ttttacagga gcagactgtc ttaaaattga tggatgaact gaatatgaaa gaaatgccga
    18181 tactgactgt ttacaataag gcagaccaga tcgacccagc gatgttcacg cctacgctat
    18241 tcccaaatgt attgatttct actcagacga aagaaggtaa gatggcgttg atcgaagcaa
    18301 tcaaacgtca gctgatggaa ctaatgaccc cttatacact ttctgtgcca agcaacgaag
    18361 gacagaaatt aagcgagttg agaagacaaa caatggtttt ggacgaagcg tacatggaag
    18421 aaacaaacga atatgaagtt aaagggtttg caaaaaccaa ttcaaaatgg ttaagaaaaa
    18481 accaataaaa ttttattttt ttaa
//
LOCUS       DAZKKW010000053        18152 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.053, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000053 DAZKKW010000000
VERSION     DAZKKW010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18152
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.053"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(248..568)
                     /locus_tag="ACG8PO_001767"
     CDS             complement(248..568)
                     /locus_tag="ACG8PO_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0207076.1"
                     /translation="MKKRLLWCLLSLSLSLTSQPVFAEETQRFPITGTRLYKNVYVQT
                     PDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            complement(1071..2189)
                     /locus_tag="ACG8PO_001768"
     CDS             complement(1071..2189)
                     /locus_tag="ACG8PO_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0207077.1"
                     /translation="MAKKDKELLSQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            2422..2544
                     /locus_tag="ACG8PO_001769"
     CDS             2422..2544
                     /locus_tag="ACG8PO_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0207078.1"
                     /translation="MHLIMIFSLLSIISLFAFSASRLLDAETQPIPVKVRKNDR"
     gene            2660..3967
                     /locus_tag="ACG8PO_001770"
     CDS             2660..3967
                     /locus_tag="ACG8PO_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HFQ0207079.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWDDKVSISDYAENLSVT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETARLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAE
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            4088..5287
                     /locus_tag="ACG8PO_001771"
     CDS             4088..5287
                     /locus_tag="ACG8PO_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFQ0207080.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYSSTWTFAVI
                     LTYFVVLEIFYSTFYFLIPLVFFSIFAFSYFSEKRRLLNGLLFNIFLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVFLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCIPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYWAQSRATLNPPILLMSGGQGADEKVPE
                     AMAMKQYAMEQGIPERDILVETNSTTTLENMRYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYIAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(5314..6582)
                     /locus_tag="ACG8PO_001772"
     CDS             complement(5314..6582)
                     /locus_tag="ACG8PO_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HFQ0207081.1"
                     /translation="MEKQLPSPVRILMYMLKWLVVTAILGVFMGSLSAFFLNSLTFVT
                     DIRLAHPWLLYLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNIAHLFRLDKAEREILVISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVWRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHAYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAVLA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAAYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRIKEKNKV"
     regulatory      complement(6638..6704)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            complement(6752..7393)
                     /locus_tag="ACG8PO_001773"
     CDS             complement(6752..7393)
                     /locus_tag="ACG8PO_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-binding protein"
                     /protein_id="HFQ0207082.1"
                     /translation="MEQTMKPYQYFFIKREVEQLLNAYSSVNDSKTVQTVQALAAEKI
                     RDVLGYEPSEIDTFLTTVLDVKLTKTQAERALEELKQIVQPFMQPSKPQIEKLFRKVK
                     KLKQPAWNEIDLQAHTYIGWNDPGTQKKFMVYYEDGKLKGISGTLSPTINKGICAICQ
                     KTSNVSLFLSTTKAGSDGTYTKKGNYICHDSDQCNQQLTKLAPFYEFAERVKK"
     gene            complement(7802..8305)
                     /locus_tag="ACG8PO_001774"
     CDS             complement(7802..8305)
                     /locus_tag="ACG8PO_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFQ0207083.1"
                     /translation="MTEKWREDEEYMSYVEDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TDLSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(8428..9192)
                     /locus_tag="ACG8PO_001775"
     CDS             complement(8428..9192)
                     /locus_tag="ACG8PO_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HFQ0207084.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKEEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAANKEISTFLVSDEVMDAVSDLPTPQGILAVMALTAE
                     GTPDFSGGWLLLDNVQDPGNVGTMIRTADAFDMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLTEVIPLMKDKGFTVYGTELNEEAHALDKVEKSDNFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            9300..9575
                     /locus_tag="ACG8PO_001776"
     CDS             9300..9575
                     /locus_tag="ACG8PO_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HFQ0207085.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            10135..11085
                     /gene="yidC"
                     /locus_tag="ACG8PO_001777"
     CDS             10135..11085
                     /gene="yidC"
                     /locus_tag="ACG8PO_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFQ0207086.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMSQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVVIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTESAKPANNKQATKQIKQPKNSTNNKGRNAGKQQKRK"
     gene            complement(11131..11532)
                     /locus_tag="ACG8PO_001778"
     CDS             complement(11131..11532)
                     /locus_tag="ACG8PO_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFQ0207087.1"
                     /translation="MSRQLVLISHGKFCEELKKSTEMIMGPQEDIYTLPLLPEDGTET
                     YKQKFLTVTDSLNDFVVLCDLLGGTPANIISKLIMEGQQITLYAGMNLPMVIEFINSQ
                     MIGSEPEFVRAGCESIVSVNELINGIAEEEE"
     gene            complement(11546..12355)
                     /locus_tag="ACG8PO_001779"
     CDS             complement(11546..12355)
                     /locus_tag="ACG8PO_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFQ0207088.1"
                     /translation="MTKSNYKLTKEDFKQINRRSLFTFQLGWNYERMQGSGYLYTILP
                     QLRKIYGDNTPELKEMMKTHTQFFNTSNFFNTIITGIDLAMEEKEGIASKETVSGLKA
                     GLMGSFAAIGDSVFAALIPTIFGALAANMATDGNPIGVFIWIFAQIVVMVFRWKQLEF
                     AYKEGISLVTTMQSRLTALTDAATLLGVFMVGALVATMINVKFSWAPTIGDVTLNMQN
                     YIDMILPKILPAGIVGGVYWLLGKKNMTPTRAIFIVLIVCVALSALGVISK"
     gene            complement(12342..13232)
                     /locus_tag="ACG8PO_001780"
     CDS             complement(12342..13232)
                     /locus_tag="ACG8PO_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFQ0207089.1"
                     /translation="MDIQWWQILLLTLYAGYQILDELQIYSSMSAPVFAGLYAGLVMG
                     DLNAGLIIGGSMQLTVLGVGTFGGASKIDANSGTILATAFSISLGMNPEQAIAAIAVP
                     VASLMIQLDILARFANTYFAHRIDRHVEDMNYQAIERNFLMGALPWSLSRMIPVFLAL
                     AFGGGLVENVVSVLNGNLKWLGDGLSVAGAVLPAVGFAILLRYLPVKKHLSYLILGFT
                     ITALLATLFSNVQLLGTSVASVVKDLPATFNSLPMLAVALIGLALAMISYKNDQNKDH
                     QPLASEPSHSVEGEIEDDEI"
     gene            complement(13245..13724)
                     /locus_tag="ACG8PO_001781"
     CDS             complement(13245..13724)
                     /locus_tag="ACG8PO_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFQ0207090.1"
                     /translation="MAIIGVRIDGRLIHGQVANLWTTKLNISRIMVVDDEVATNTIEK
                     SGLKLATPAGVKLSILPIEKAATNILAGKYDSQRVLIVAKKPDRILSLVEKGVPIKEV
                     NVGNMSQTNETRSITKSINVVDADIEVFKKLDQLGIHLIAQMVPNDKAEDFMNLLIK"
     gene            complement(13900..14841)
                     /locus_tag="ACG8PO_001782"
     CDS             complement(13900..14841)
                     /locus_tag="ACG8PO_001782"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hexose kinase"
                     /protein_id="HFQ0207091.1"
                     /translation="MITTVTLNPSIDIAYTIEELNLDKVNRVETVNKTAGGKGVNVAR
                     VLNLLGEEVIATGLIGGQHGEYLKNQLDQLGIMHQFVPIKGETRDSIAILHSSRQTEL
                     LERGPFITKKEQQTFTDHFEQLLEISDIMTISGSLPQGCPLDYYKQLVALADKKGKDI
                     LLDTSGKFLLEVLEGKEKPLLIKPNLEELGELIGQRICSSIQLEKIQSLLAHPIFEGV
                     PWVVVSLGERGAVAKVAQCFYEVIIPKISVVNPVGSGDATLAGSAHALSKRQSIEEIL
                     AFSMACGMANAQQEKTGWIDPMKVTMLMKHIQVKKIK"
     gene            complement(14838..15833)
                     /gene="lacD"
                     /locus_tag="ACG8PO_001783"
     CDS             complement(14838..15833)
                     /gene="lacD"
                     /locus_tag="ACG8PO_001783"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009025 - tagatose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HFQ0207092.1"
                     /translation="MNEEKNRAMERLSTKDGIISALAIDQRGALKKMIKSLGKEPSED
                     QISEFKELVSAELTPYASAILLDPEYGLPAAKVRAKETGLLLAYEKTGYDATTPGRLP
                     DLLADWSSLRLKEQQADAVKFLLYYDVDEAPEINHLKHVFIERLGSECEGENRPFYLE
                     LVSYDAQISDSSSPEYAKVKPHKVIEMMKEFSDPKYKVDVLKVEVPVNMNYVNGYSTG
                     EFIHSKEEAAAFFKEQSEATPLPFIFLSGGVSTELFQQTLIFAHESGSTFNGVLCGRA
                     TWKDGVKAYVENGKDGAIHWLRTNGRENIETLNKVINETATPWFNKTKSKKVKMK"
     gene            complement(15826..17022)
                     /locus_tag="ACG8PO_001784"
     CDS             complement(15826..17022)
                     /locus_tag="ACG8PO_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFQ0207093.1"
                     /translation="MFELSKNKLKQIGAAITTQEIKQQPDLWKETIDRLMQSQENLAL
                     FFDQIKKEANGRRIRVIFTGAGTSQYIGDTLLPYLNEHGDNQFFIFHSFGTTDIVASP
                     KEFLFPNEPTLLVSFARSGNSPESVAAVQIANEQVETLFHLTITCAKEGMLAKNAQDD
                     KQNYLLLMPERANDAGFAMTGSFTCMLLSALWIFDQTAMEKKERYLEQLVKIGQDLLS
                     RDQEIQKLLPDFFERIAYLGSGSLAGATREAQLKILELTAGKVATIFDSSMGFRHGPK
                     SFLNSETLVVGFVSNQPYTRNYDLDILEEIKEDRVTANVIAIAQPGTIDFSGMTVLLE
                     DSVSKLPDAYLALAMIMTAQIIALYTSIKVANKPDTPSPTGTVNRVVKGVIIHKYETE
                     GEMTDE"
     gene            complement(17040..17768)
                     /locus_tag="ACG8PO_001785"
     CDS             complement(17040..17768)
                     /locus_tag="ACG8PO_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0207094.1"
                     /translation="MESSNKGKSLYYQLVDLLKDRIETQMTPHDKLLSERELCQHYGV
                     SRTTLRLALQELENQGYIYRRHGKGTYVSDLSDKSSNLAGAYSFTEEMREIGRVPKTV
                     ILEFRQMEANKRIAKNLNIHLGETVFKLKRLRLADEEPLMVERSYLPAKLFMGLDLPL
                     LESKPLYDVFSQDYEQQVQLADEEFYASIVQKKDAVVLRIADGAPVLHLTRITYNVKN
                     EIIEYTDSVARADQFHYKIRHIRK"
BASE COUNT         5456 a         3853 c         3095 g         5748 t
ORIGIN      
        1 ccgaactttt ttttaaaata aacgacatat tgttcagttg gtgttttttt gctctgatgt
       61 tcttttttaa actaaaaaaa ttattctttc tgactactta tcggttctgt ttcaacggtc
      121 gaggctgggg aagattcttt agtaggtctg atgttctcat ggtatttctg atagcctatg
      181 acagttacca gaagaacaag gcaaaccaat acgaccaacg agattagttt ttcttttttg
      241 ctcatatcta tacctcataa ataacaggat gatgtccatc atatttctga gtatgagtaa
      301 aagcaaaagc tgcagctgta gaatgtaaga aatgccagca actttcataa tcacaggtcg
      361 gacagtacca aaagtaacca aatcttggct gcaaagaccg ttcttctgtt tcaggaatcg
      421 cagaatcaag atctggtgtc tgtacataaa catttttgta cagacgtgtg cctgtaatag
      481 gaaaccgctg tgtttcttca gcaaaaactg gctgacttgt taaacttagg gatagagaca
      541 gcaaacacca taacagtctt tttttcataa agacctcctt atcgataagt ttttcccaat
      601 taaaatcata tctcaagaac gcccgttatt cacattttgt tcataaatga tgttttttgt
      661 atcataatcg tcaaaaagag gtcgtgacca cgtcacaacc tcttttttaa acggtgttcc
      721 agaagtagct tcttcggaaa taagccgaaa tttcacaaaa atttgagaaa taatttttgt
      781 caatctcttc ttatttcttg aagctgacca cttctgtcac aacctcttcc ttaaacggtg
      841 ttcaaaagct tgcatctgcg gtaataagcc caccaaacaa aaaatatgaa gagctatttt
      901 tgcttgctgt tcttattact tgatgcagaa cagctttttc acaacctctc atacacggtg
      961 tttaatcagc tgaccttcgg tattagctca aaaaacggca aaatatgtga aactattttt
     1021 ccgtttgttt ctctaatact caggtcaaaa cactgatttc acaacctctt ttaataacga
     1081 agaatcgctg tcagactctt ttcatctggc gcatcttttt gatccaaaat aaacactttt
     1141 ccgccattaa tgatcacatc atctgctaat gcattcaaca cttgacgccg atcatattct
     1201 gtttcaggat cttcaccgaa accatcagct aagtttgacg tagcgataaa taaatgtgac
     1261 acttttcctt ctttcgcagc tggaacgata tcaactaact gatcgatgaa tttcttatct
     1321 aagagtttat tgtattcttt tgattcttga gctgcgagtt cacttgacag cttcttcgcc
     1381 ccttcttcga tatctctaaa ggatagctgc gcaggagaag cagaaatcgc aacgtctgca
     1441 caataatagg gatttttagc aatttttttg aacatcgtct gattttctgg tagagcaaat
     1501 aaataaatcg gccattgttc tggattttcg aaagaatcct tcaagtacgt atcaactgct
     1561 tggtaatagt tgacccaatc gatctctact tcttcatctt ttggatttac gccatggaaa
     1621 gttactccat ctcgggctcc gcctcctcca ttagaagaga agttcaaacc gccacctgtc
     1681 agttcagatc ccaatgcagt aacgacatca accggtgccc cttctggtag agatacttct
     1741 tttacttgtt tgttgctgac ttcatacaat ttcatggagt cgcggttcaa tcccaataaa
     1801 taataagaat agttgaactg cccatttttg ataatagcca gtaaataagg aagatcactt
     1861 acatagtatt gatcgtctac cgggatactc aaacgatgga tataggtttg gtcagcagtt
     1921 ataatgaccg ctacactcgt agttgcacta cgccaaaacg aaggatctcc taacaaattt
     1981 tcaatttttt cttggaacgg ggaccagtca tgttcggaat attttttctc aaaacgtttc
     2041 tttgcttctt tcgcaaaatt tttgaatttg atctgatctt tttccacatc ttgatgggcg
     2101 acatgcgtat tcagcatgat cgtgacgaat ggtcctttag cgccttctga agtcaattgt
     2161 gacaataatt ctttatcctt ttttgccata aacgtacccc tcctaaaaaa atcattcctt
     2221 tgtttctatc taaagtctac cacaacacta tttttccata caataaaacg tatctaaaaa
     2281 aataaaacgg tttctacggt tttaaattta gctatttttt taagttatct tcataatttc
     2341 ctcaattatt tagccccagt cttcaactat tcttcatgaa atgctgataa actaagaaaa
     2401 agaataaaag aggtggatct tatgcatttg attatgattt tcagtttatt aagtattatc
     2461 agtttatttg ctttcagcgc ttcccgctta ttagatgcgg aaacacagcc tattcctgta
     2521 aaagtacgta aaaacgaccg ctaaccttcc ataacgtgcg ttcttatcct aaagtttttt
     2581 cagaaacatt gaaactctct cccctctctg ataaaatttt aatttgtagc gaaacaaatt
     2641 aattacaagg agatcggata tgtccataaa aaaaacacgt ctagtcctat tattggctag
     2701 tctcattctt tgccttgctt cttttctacc agctgttgct gtgcaagctg aagatacttt
     2761 caaagtaaat gcaaaagcag cctttgctgt agatgcgcaa agcggtaaga ttctttatga
     2821 tcaagatgga gaaaaaacca tggggatcgc ttcaatcaca aaaatcatcg gtctttacat
     2881 cgtccttgat caagtaaaag aagggaaact atcatgggat gataaagttt caatttcaga
     2941 ttatgcagaa aatttgagtg tcaccccaga tttatcaaat gttcctttgc ataaagaaaa
     3001 cacctacacc gttaaagaat tattcgactc tgcaatcatc cagtcagcca acgcttcaat
     3061 ggttgccttg gcagaaaaaa tctctggcag cgaagcaaaa ttcactgaac gaatgaaaga
     3121 acaattgaaa gattggggta tcaaagatgc aacgatcgtt aatgcatcag gactgaataa
     3181 ctcttatctt ggcgaaaacc gaccagaagg tactggagaa aacgacgaaa atcaaatgtc
     3241 tgctcaagat gtggctatcg ttgcccgtca tttgatcttg gattttccag aaatactgga
     3301 tgtatctagc actacgacac aaatgtttgg cgaaaacacg caatcaccag tagaaatggt
     3361 caactggaac tggatgctcc caggattcgt gaattataaa gaaggcgtgg acggattaaa
     3421 aactggtacg actgaacttg caggagcctg tttcgtcggt acgatcacta aaaacgatca
     3481 acgaatcatc actgtcgtat tgaatgcaga cggccatgcg gaaaacccaa gtgcccgttt
     3541 caatgaaaca gctcgcttaa tggattattg ctacgacaat tggtcagaaa aagagcttgg
     3601 aaaagccaat gcaagtatcc ctagtctaaa aaccattgat gtaaaagacg gaaaagaatc
     3661 aacagtgccc gttgtgctta aaagtcctgt aaaagtatgg gtacggaacg atatggatac
     3721 tggaaaatta acaatcacac caactcttga caaaaaacaa gtcactgaca atgagatcca
     3781 agcacctatg gataaaggaa caaaaatcgg taccgcaacg atcaccttgg cagaagataa
     3841 attaggttat cttgaagaca acaacgcgcc atccacagaa atcatcacgg ataaaaccgt
     3901 agaaaaagca aatattttcg ttattggctg gcgacgcgtc gcagaatttt tcggtaactt
     3961 gttttaattg aacaaaaaga aaaaagcatc tttccatgtg gaagatgttt ttttgctttt
     4021 tctagtttaa ataacagctt ttttcatcta ttttcgttat actgtataag aaaaaggagt
     4081 tgaatttatg aatctaacca gagatctgat ttttttggtt ctctatctta ttggggtatt
     4141 ctccatcgtt tattggcgaa aacagaaaaa ggatccttcc ttgttctttt acagcagtac
     4201 atggacgttt gcggtaatct tgacttattt tgtcgtgttg gaaatttttt attcgacctt
     4261 ttattttttg attcccttag tgtttttcag tatctttgcc ttttcctatt tttcagaaaa
     4321 gcgaaggctg ttgaatggtc ttttattcaa tattttcctc attagttttg gtatctactt
     4381 gtttgtcttg ctatacgaaa cacaaaatat ttttcttggc ggactgattg ccttgatcac
     4441 gatcccgtta ctgcttgtct ttctattcgg catttatgga ttgatcgttt ttcttttttg
     4501 gaacggtgtc acagttttac gtagagagtc gcattcatta gctaatcttt taacgttgat
     4561 actagccatt tttcttacat tgtttcttgt ttttgacttt ttcttgttaa aatatttacc
     4621 acaatggata aatgctttgt ttttctgcat cccattgatc ttgatctatc tatttatcgt
     4681 attctacaac tttttgactg tctcattctt gtatcagttc aaccgccctc gatacaacca
     4741 agacttcatc gttgtgttag gcgccgggtt gatcaatgga gaaacagttt ctcctcttct
     4801 tgccaagcga atcaataagg cgattgcttt ttattgggca caaagccgtg cgacattaaa
     4861 tccgcctatc ctattaatgt cgggtggaca gggagcagac gaaaaagtac cagaagcaat
     4921 ggcgatgaaa caatatgcca tggaacaagg aattcctgaa agagatattc ttgtcgaaac
     4981 caattcgaca accactttgg agaatatgcg ctattcaaag gaaatcatgg atcagcaaat
     5041 gaaaggtccc tatcgcgcga tcttcagttc caacaattac cacattttca gagctggtct
     5101 ctatgcacga caagcgaaac tgaaggcaaa tggcattggg gcaaaaacag ctttctatta
     5161 cttgcctaac gcgtttttaa gagaatacat cgccatcttg atgatgaaca aaaaaagaca
     5221 tatgatcatt tgcggattgc ttttcgcagg ttccgcattc ttatcactga tcactttact
     5281 tttctaaaaa aacaaggtgt gacaaaacat atgtcacacc ttgttttttt cttttattct
     5341 cttgacgatt ggcaagaata gtattccttt tttgacggct acttgctgtg cagaataaat
     5401 accgctgttt cctgaacaca taaagctgat caagcagatc ataaagaagt aagcagcagc
     5461 ttgtgaacca aataattcga ttcccatcac aaaacaggca atcggtgtat ttgtcgcacc
     5521 agaaaatacc ccgataaaac ctaatccagc aagaaaagga acagaaacat ggagcaaagg
     5581 cgctagtact gcgccaagcg ttgcaccaat ctcaaatagt ggtgtcactt ctcccccttg
     5641 gtaccccgca cctaaagaca agaccgtgaa gaataatttc ccaataaaat cataagcatg
     5701 tgcctgtcca ttaaatgatt cctgcagtaa cggaagactc aacccaaggt aacgttgtgt
     5761 ttgcaaaatc aacgctgcgg ctacaactac tgctccacct atcgtatttc gccaaacagg
     5821 atgaggaatc cattgcgcat accgcgcttt taaaaagacg atcgaacggc tgaataccca
     5881 accaatcaaa ccaaaagcaa tcccagcaac aaaaactttg aagaacagca acatactcca
     5941 ttcaggaatc acacccattt ggtaatgtag atgagaaaca ccatagcttt ctgtcatgaa
     6001 atttgcaaaa agcgcagcaa agaagctagg aaatattgct tctgttcgga ctttaccgat
     6061 tgccaacact tctaatccaa ataatgttcc agctaaaggt gtgccaaata cagaactaaa
     6121 acccgcactg attccactga taaccaaaat ctcccgttct gctttatcca atcggaataa
     6181 atgcgctata ttatcagcta atgctccccc catttgaact gctgttcctt cacgaccaac
     6241 agatccccca aataaatggg tagtgatggt cccaaacaag gtcaatggaa tgagccgcaa
     6301 agggatcttc tcttctcctc cattcccttg atcgatcact aagttattcc cacgactaga
     6361 aagaccgcca tgatacgcat aaagataagc aaaaagcgcg ccgctgactg gcaatagata
     6421 aagtagccaa ggatgtgcta gtcggatatc tgtcacgaac gtcaggctat ttaagaaaaa
     6481 agcagacaag ctccccatga atactccgag aatcgctgtg accaccagcc acttcaacat
     6541 gtacatcaaa atacgcactg gactcggtaa ttgtttttcc ataaaaaatt cctcctcaaa
     6601 taaaaaaacg cctactaaag aaaggggctg gccccagact ttagtaggcg tcattagcta
     6661 tcttacgtta gcatttaaag gcgaacacca tcacctattc acttccatta aaagatagca
     6721 tatttttctg taaaaaacaa taaaattatc gttatttctt tacacgttca gcaaattcat
     6781 agaaaggagc caatttcgtt aactgttgat tacattgatc actatcatga caaatataat
     6841 tccccttttt cgtatacgtg ccatcagaac cggctttcgt tgtagaaaga aagagcgaga
     6901 cattcgatgt tttctgacag atcgcacaga ttcccttatt aatcgttggt gataaagtac
     6961 cgctgatccc ttttaatttt ccatcttcgt aataaaccat gaattttttt tgcgtccctg
     7021 gatcattcca accgatgtac gtatgtgctt gcaagtcgat ttcattccaa gcgggttgtt
     7081 tgagtttttt cacttttcgg aataatttct cgatttgtgg tttgctcggc tgcataaacg
     7141 gctggacgat ttgcttcagc tcctccaatg ctcgttctgc ttgcgttttt gtcagtttta
     7201 catctaaaac tgtcgttaaa aacgtatcga tttctgatgg ttcgtaacct aatacatcgc
     7261 gaatcttttc agctgctaga gcctgaaccg tttgtaccgt ttttgagtca ttgacagatg
     7321 agtacgcatt taataactgc tctacttctc tttttataaa aaaatattga tatggtttca
     7381 ttgtttgttc catgatttta aagtcctttc ttctaaccaa agtgcatact ttgtagaaaa
     7441 aagtgatctt ctgcaaagta tgtgttacct gaagatcccg gactctttta aataagatac
     7501 gttttgcatc ttatcatcac cccctttaat gttcattctt ctatgtacat gattcattcg
     7561 ataaataaaa gtaaaccgta tcttgagcag atagtcaact gcaaacagaa aaaggactgt
     7621 gacaaaagtc gtgtcaaagt ccctttctac aaacaaacgg tgttccagaa gtagcttctt
     7681 caaaaaataa gccggaattt cacaaaaatt tgagaagcaa ttttcgtaaa ttccttctta
     7741 tttcttgaag ctgacccact tctgtcacaa cctcaatatg gtctattatt ggttttacct
     7801 gttaaatcga ggattctttt ccgaagtaac gttgccatct cttacgcata cttgctgaca
     7861 taggctgagc ggcttctttg actgcgcagt atttgtctac gaatgttacg atatatcctt
     7921 ctttgtactt aggcggtgca atcgttgcgc cccacatgtg cttcaaaatg atatctcgct
     7981 caagatccga caaatcggtg atcttttctg cattctttac agcgattctt ggatggatat
     8041 acgcatgtgt gccttcatca aatttcgtcg tacgccaatc atagtagaac agatcatgca
     8101 aaagtccagc acgcgctgtt gctttagcat tgccacccca tttttttgcg agcagataac
     8161 tatagtaaga aacactgatg gaatgctcca aacgagtgga atgaacatgc tgtgtgtaat
     8221 tcgctaattt tttcactgct tctgtttcta aaagatcttc tacataagac atatattctt
     8281 cgtcttcacg ccatttttct gtcataatta tcgatctgcc acctttttga atttcatttt
     8341 cttctgaaat tgatctaagt ataccaccag tgttcggatt attctagtcg ccttggataa
     8401 aatagccaaa gcttaatagt tccttaatca taaagaaaaa tgataaagaa caatccctgc
     8461 cgcaacccca acattcaacg attccgcatt tcctttcatc gggatatata cgttctgatc
     8521 agtcaaagac aagatttcct gacttacccc ctgaccttcg ttccccatga ttattgcgaa
     8581 gttatctgat ttttctactt tgtctaaggc gtgtgcttct tcatttaatt ctgtgccata
     8641 cactgtaaac cctttgtctt tcattaaagg aatcacttct gtcaaatctt tttgcaacac
     8701 aggcaaatga taattgcttc cctgcatgct gcgcagcacc tttgtactat aaatatctgc
     8761 cgttcctttg cctaataaaa caccagccat atcaaatgca tctgccgtac ggatcatcgt
     8821 ccctacattt ccaggatctt gtacattatc taataacagc catcctcctg aaaaatctgg
     8881 cgtgccttca gccgttaacg ccatcactgc taagattcct tgtggtgtag gtagatccga
     8941 tacagcatcc atgacctcat ccgaaactag aaaagtagaa atctctttgt ttgcggccag
     9001 ccagtctccc cattctttca tgcctcgctg tgtcacaaaa agttctttta tttctgcatc
     9061 agacgacgca gcttcttcaa tcagatggaa cccttccaaa agatattctt ccttttcttc
     9121 ccgatatttc tttttatgta gtttttttaa ttctttgatt cttggatttt ttgctgattg
     9181 gatctctttc acattcctca cctccccatg attatatcat ggaataggtg cttgagcttg
     9241 tttcatccta aaaaaatcgg tatcattaat ttataaaata ggaaagaaag gtgacgaaca
     9301 tgcgcaaagt taaaatgaac gtccaaggac gcgtccaagg cgtaggattt cgctacatga
     9361 ccaagatggt cgctgatcaa ctaggcgtga ctggaacggt aaaaaacgaa gatgacggct
     9421 cagtcacgat cgaagcaatc ggcaatgacg acatcatcca gaaatttatt gaagaagtca
     9481 agaaatctcc gagcccaagt ggacgagtgc agtatgtgga tgtccaagaa gacccgatga
     9541 ttgaggaacg aaagaaattt gatgtggtgg gataaggttg tgaaaaagct ggtctgcatc
     9601 aagcattaag gacaggttgt gaaaaatagc tctccatatt tttcaacagg ctggacttaa
     9661 tgccgtagat gcaagctttt gaacaccgtc aataaggttg tgagaaagtc gcttagtttt
     9721 gagtagtaag gagaattttg tgaaaatggc tttccacatt tttgcataaa atttgactta
     9781 ataccgagaa actgactttt gaacaccgtc gataaggttg tttttgagac gctttgcctc
     9841 aagtattaaa gataggtagt aaaaaagctg atctataact tgcaataaat ttgcttaacc
     9901 catattttcc agacagaaca aaaaagtttt tagtttttta aataagaaag aaacacttgg
     9961 aatcactctt ttctttccaa gtatttcttt cttgattttt tgtctttttt tcaaaatttt
    10021 tcgctacgta aaagaacgaa tatttatatg aaatttctat gaagttgatt gaattccatg
    10081 cgctttttta gtatagttaa gtttgtgtaa aaattataga agaggaagta ttctatgaat
    10141 aaatggaaaa actggttatt gggatccgga cttgtcagta tcctgctctt tttatctggc
    10201 tgtgtgcgga tggattcaaa cggaaaccct gacacttcgg gaatcgttta tcgagtactc
    10261 gtccatccta tgagccaagc gattacatat ctagttgaga atttcaattg gtcttatggc
    10321 tgggcagtta ttgcaatgac tgtgatcgtc cgtatcatca tcttgccact aggtattagt
    10381 caatcgaaaa aaacaatgat ccaatctgaa aaaatgcaag cgttaaaacc acaagttgaa
    10441 gcagcccaac aaaaattgaa agctgcaaca acacgtgaag aacaaatggc agcacaagca
    10501 gaaatgcagc aagtataccg cgaaaacgga ttgagtatga ccggcggaat cggctgtctg
    10561 cctctattga tccaaatgcc gatcttctct gccttgtact ttacggcacg ttatacagaa
    10621 ggtattcggg aatcatcttt ctacggcatc gatttaggct caccaagtat ggtacttgtt
    10681 gtcattgcag gggtcgccta tctgcttcaa ggatacatct caacgatcgg tattcctgaa
    10741 gaacagaaaa agacgatgcg tacgatgcta atcgtctctc ctgctatgat cgtcttcatg
    10801 tcgatcagtg cgccagccgg tgttacactt tactgggtag tcggtggtgt gttcagctgt
    10861 ctgcaaacat tcatcacaaa cgttatgatg aaaccacgtt tgaaagcaca agtggcagaa
    10921 gagttgaaac aaaacccgcc aaaacaagtc gtcactccaa gaaaagacgt gacagaatca
    10981 gcaaagccag ctaacaacaa acaagcaaca aaacaaatca aacaaccaaa aaacagtaca
    11041 aacaacaaag gccgcaacgc aggaaagcag cagaagcgga aataaaaaca taggacaaaa
    11101 aagatgagag ctaagccctc atcttttttg ttactcttcc tcttctgcta ttccattaat
    11161 taattcgttg acagagacaa tgctttcaca tcctgcacga acaaactcgg gctctgaacc
    11221 aatcatttgt gagttgataa attcaatgac catcggaaga ttcatcccag cataaagagt
    11281 aatctgttgt ccttccatta ttagtttaga aatgatattt gcaggtgtac cacctaaaag
    11341 atcgcatagt accacaaaat catttaacga atctgtgaca gtgagaaact tttgtttgta
    11401 tgtttcagtg ccatcttctg gtaataaagg taacgtgtag atatcctctt gaggccccat
    11461 gatcatttct gtactttttt tcaattcctc acaaaatttt ccgtgactaa tcaataccaa
    11521 ttgtctactc atttttcgct ctcccttatt tcgaaatcac acccaatgca gataaagcaa
    11581 cacacacaat taatacgata aaaatcgctc gagtaggagt catgtttttc tttcctaata
    11641 accaataaac accaccgacg atcccagcag gtaaaatttt aggtaagatc atatcgatat
    11701 agttttgcat attcaatgtc acatcgccaa tcgttggtgc ccatgaaaat ttaacattga
    11761 tcattgtcgc aactaatgcc cctaccatga atacccctaa taaagtggct gcgtccgtta
    11821 atgcagttaa gcgagattgc atagtagtaa ctaaggaaat tccttctttg tatgcaaatt
    11881 caagttgctt ccaacgaaaa accatgacaa cgatctgagc aaaaatccat ataaacacac
    11941 ctatcggatt tccatcagtt gccatattcg ccgccaaagc cccaaaaatc gttggaatca
    12001 aagcagcaaa tacggaatct ccaatcgctg caaaggaacc catcaatcca gctttcagcc
    12061 ccgatacggt ttcctttgaa gcaattccct ctttttcttc cattgcaaga tcaatcccag
    12121 taatgattgt attaaaaaaa ttagaagtat taaaaaattg cgtatgtgtt ttcatcatct
    12181 cttttaattc tggtgtattg tctccatata tttttcttag ttgtggcaag atagtatata
    12241 aatagccaga accctgcatt ctctcatagt tccaacctaa ttgaaaagtg aacaaacttc
    12301 ttcgattgat ttgcttaaaa tcttcttttg ttaatttgta attagatttc gtcatcttcg
    12361 atttccccct caacagaatg tgatggctca ctagctaatg gttgatggtc tttgttttga
    12421 tcattcttat aactgatcat tgctagtgct agaccaatca atgctacagc aagcatcggc
    12481 agcgaattga aagttgccgg aagatcttta acaacgcttg ccacacttgt tcctaataat
    12541 tgaacattag agaataacgt tgctaataat gccgtgatcg taaatcctaa aataagataa
    12601 gatagatgtt tttttactgg taaatagcgc agaagaatgg caaacccaac agctggtaaa
    12661 acagctcctg ctactgaaag tccatcgcct agccatttta gattcccgtt taaaacagaa
    12721 acaacatttt caactaatcc accaccaaat gctaaagcta aaaatacagg aatcatacga
    12781 gagagcgacc aaggtaaagc tcccattaag aaattgcgtt cgattgcttg ataattcatg
    12841 tcttctacgt gacggtcaat acgatgggca aagtatgtgt tagcgaatct agctaaaata
    12901 tccaattgga tcattaagct tgctacaggc actgcaatag cagcaatcgc ttgttctgga
    12961 ttcattccaa gagaaatcga gaaggcagtt gctaaaatcg tcccagagtt ggcatctatt
    13021 tttgatgcgc ctccaaacgt ccccactcct aaaactgtca actgcatact gccaccaata
    13081 atcaaacctg cgtttaaatc acccattact aaacctgcat ataaccctgc aaacactggc
    13141 gcactcattg aagaatagat ctgtaattca tctaaaattt gataacctgc atataatgtt
    13201 aataataaga tctgccacca ttgaatatcc atggatcatt cctcctattt gattaaaaga
    13261 ttcataaagt cttctgcttt atcgtttggt accatttgag caattaaatg tataccaagc
    13321 tgatctaatt ttttaaatac ttcgatatct gcatctacca cattgattga ttttgtaatg
    13381 gaccgtgttt cattcgtttg tgacatattc cccacattta cttctttgat aggaactcct
    13441 ttttctacta aagataagat tcgatctggc ttttttgcta cgatcaatac tcgttgagag
    13501 tcatatttgc cagctaaaat atttgttgct gctttttcga ttggcaaaat gcttaatttt
    13561 acccctgctg gagtcgctaa cttcaatcca cttttttcaa ttgtgtttgt ggctacctca
    13621 tcatccacga ccatgattcg actaatattc agtttcgttg tccaaagatt tgctacttgt
    13681 ccatgtatca aacgtccatc gattcttaca ccaataattg ccatcatgca ttctccttat
    13741 ctattttttc tatttattgt tcaattaaaa ctgtgacatc atcaaatatc ccttctgttt
    13801 caaagataac gttttcattt tttctttctt atatttcatt tttaagctaa gaacaaattc
    13861 cttcccaact aaagaaaacg ctttctttta tatttaagtt catttgattt tcttaacttg
    13921 aatgtgtttc atcaacatcg taactttcat agggtcgatc cagccagttt tctcttgttg
    13981 cgcgtttgcc ataccacaag ccatagaaaa tgcgagaatc tcttcgattg attgcctttt
    14041 tgataacgca tgggccgatc ctgcaagggt cgcgtcacct gaaccaacag gattaacaac
    14101 tgatatttta ggaataatga cttcgtaaaa acactgagca acttttgcca cagcacctct
    14161 ctctcctaaa gaaacaacaa cccatggcac tccttcaaat attggatgag ctaataagga
    14221 ctgaattttt tctaattgaa tcgaagaaca gatacgttgg ccaatcagtt ctcctaattc
    14281 ttccagattg ggcttgataa gtagtggctt ctccttacct tccaatactt caagcaaaaa
    14341 tttaccagaa gtatctagta atatgtcttt ccctttttta tcagctaaag caaccaattg
    14401 cttatagtaa tcaagagggc atccttgggg taaactacct gagatcgtca taatatctga
    14461 tatttcgagg agctgctcga aatgatcggt aaatgtctgt tgttctttct ttgttatgaa
    14521 ggggcctcgt tccaaaagct ctgtttgtct cgaactatgc aggatagcaa tagaatccct
    14581 tgtctctcct ttaattggaa caaactgatg catgatccct aactgatcta gctggttctt
    14641 tagatattca ccgtgctggc cacctattaa accagtagct atcacctctt ctcctaataa
    14701 atttagaacc ctcgccacat tcactccttt tcctcccgct gttttattga ctgtttcaac
    14761 tcgattgact ttgtcgaggt ttaattcttc aattgtatac gcgatatcga tggaaggatt
    14821 caaggtaact gtcgtaatca tttcatcttc acctttttgc tttttgtttt attgaaccaa
    14881 ggggtagcgg tttcatttat tactttattt aaagtctcga tgttttctct cccatttgtt
    14941 cttaaccaat gaatagctcc gtcttttcca ttttcgacgt atgctttgac cccatctttc
    15001 catgtcgcac gtccacacaa tacaccattg aaggtggaac ccgattcatg agcaaaaatc
    15061 agtgtttgtt gaaataattc tgttgaaaca cccccactca aaaaaatgaa tggtaatgga
    15121 gtcgcttcac tttgctcctt aaagaaggca gcagcctctt ctttgctgtg aataaattca
    15181 cctgtggaat agccattcac ataattcata ttgactggta cttctacttt caacacatca
    15241 actttatatt ttggatcaga aaattctttc atcatctcaa taactttgtg gggcttgact
    15301 ttcgcatact ctggagaaga actatcgctt atttgtgcgt cataagaaac taactccaaa
    15361 tagaagggac gattttctcc ttcgcactca ctacctaaac gttcgatgaa cacatgcttc
    15421 aaatgattga tttcgggtgc ttcatcaaca tcgtagtaaa gtaagaattt tactgcatcc
    15481 gcttgctgtt ctttcaatct gagagaagac caatccgcta ataaatcagg tagacgacca
    15541 ggcgtagttg cgtcatagcc ggttttctca taagctaaca ataaccccgt ctcttttgct
    15601 ctgacttttg ctgctggcaa tccatattcc ggatccaata aaatagcaga agcatagggt
    15661 gtcagctcag ctgatactag ttctttgaat tcactgattt ggtcttcact tggctctttt
    15721 cccaacgatt tgatcatttt ttttagagca ccacgctgat cgatcgctaa tgcactaata
    15781 atcccatctt ttgttgatag tctctccatt gccctatttt tttcttcatt catctgtcat
    15841 ttctccttcg gtttcatact tatgaataat gacgcccttg actactcgat tgaccgtgcc
    15901 tgttggagat ggtgtatctg gtttattagc tactttgatt gaagtataga gtgcaatgat
    15961 ttgtgcagtc ataatcatcg ctaatgcaag ataggcatcg ggcaatttac tcaccgagtc
    16021 ttccaatagc acagtcatac ctgagaaatc aatagtaccc ggttgcgcaa tcgcaatgac
    16081 attggcagtg acccgatctt cttttatttc ctctaaaata tctaagtcat aatttcgcgt
    16141 atatggttga ttgctcacaa aacctacaac gagtgtctcg ctatttaaaa aagactttgg
    16201 tccatgacga aaccccatcg aagaatcaaa aattgtagca actttacctg ctgttaattc
    16261 taaaattttt aattgtgctt ctcttgtcgc acctgccaaa cttcctgaac ccagataagc
    16321 gatccgttca aaaaaatcag gtaataactt ttgaatttct tgatctcttg aaagaagatc
    16381 ttgaccgatt ttaactaatt gttcaagata tctttctttt ttctccattg ctgtttgatc
    16441 aaagatccat aaagcactca ataacataca cgtaaaactt cctgtcatag caaaacctgc
    16501 atcgtttgct ctctctggca tcaataacag ataattctgt ttatcatcct gtgcgttttt
    16561 tgccaacatc ccctctttag cacaagtaat cgtcaaatga aacaatgttt caacttgttc
    16621 atttgcaatt tgcacagccg ctacactttc tggactgttg ccactgcgag caaatgaaac
    16681 taataaagtc ggttcatttg gaaacaaaaa ttcttttggt gaagctacaa tatctgtcgt
    16741 gccaaaactg tggaagataa aaaactgatt gtctccatgt tcatttagat agggtaagag
    16801 cgtatcccca atatattgag acgttcctgc acccgtaaaa ataacacgta ttcttcttcc
    16861 gtttgcttct ttttttattt gatcaaaaaa tagagctaaa ttttcttggc tttgcatcaa
    16921 tctatcgatt gtttctttcc agagatctgg ttgttgtttt atttcttgtg tcgtgattgc
    16981 tgcacctatt tgttttaatt tgttcttcga tagctcaaac atgttgttct ccctctcaat
    17041 cattttcgga tatgccgaat tttataatga aactggtccg ctcttgctac actatctgta
    17101 tattcgataa tctcattttt cacgttatag gttatcctcg tcagatgtaa gacgggagcg
    17161 ccatcagcga ttcgtagcac aacagcatct tttttttgca cgatacttgc ataaaactct
    17221 tcatcagcta attgaacttg ttgttcataa tcctgtgaaa acacatcata caacggttta
    17281 ctttcaagta acggcaaatc aagtcccata aatagtttcg ccggtagata agaacgctca
    17341 accatcaatg gctcttcatc tgccaaacgc aatcttttaa gtttaaatac agtttcacct
    17401 aaatggatgt tcaaattctt agcaattctc ttattagctt ccatttgtct aaattctaaa
    17461 atgaccgtct tagggacgcg ccctatttcg cgcatttctt ctgtaaagct atatgctcca
    17521 gctaagttcg atgacttatc agacaaatca gagacatagg taccttttcc atgtcttcgg
    17581 taaatataac cttgattttc caattcctgt aaagccagac gtaaggtggt acgactcacc
    17641 ccatagtgtt gacaaagttc tctttcagat aacagcttat catgcggagt catctgtgtt
    17701 tcgatacgat ccttcaataa atcaactagt tgataataca atgattttcc tttatttgag
    17761 ctctccataa aaatcacctc ttctttactg gttataacca caacacaaga atagtgaatt
    17821 attaatcatt tgtcaaaggt tttttagacc aaaaaagcta ttataacagc tctttaaagc
    17881 tttatatctt gaatagacaa agatacctca aaaataccag ttaccaataa aagaacagaa
    17941 aaaaagacga accacataag cgatccgtca aaatggtcta acttatcgag cagcgtgatc
    18001 cacattaatc cctatgatat tcctacaact atcgttttat tacttccaat atcatttaca
    18061 acaactaaaa atagcttatt aatgacaaat gttattatta gttttagata acttttgcag
    18121 ttttatactt tttttctata aaatttgaca aa
//
LOCUS       DAZKKW010000054        17819 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.055, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000054 DAZKKW010000000
VERSION     DAZKKW010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17819
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.055"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            108..920
                     /locus_tag="ACG8PO_001786"
     CDS             108..920
                     /locus_tag="ACG8PO_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HFQ0207095.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            917..1717
                     /locus_tag="ACG8PO_001787"
     CDS             917..1717
                     /locus_tag="ACG8PO_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFQ0207096.1"
                     /translation="MKQRVITAAVALVLFIPIIWIGGIAVELTAAILAVVGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            1878..3146
                     /gene="rseP"
                     /locus_tag="ACG8PO_001788"
     CDS             1878..3146
                     /gene="rseP"
                     /locus_tag="ACG8PO_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HFQ0207097.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAIVLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPGKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            3214..4923
                     /locus_tag="ACG8PO_001789"
     CDS             3214..4923
                     /locus_tag="ACG8PO_001789"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFQ0207098.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPKRDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTSLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELANTLPILLN
                     QGE"
     gene            5135..9487
                     /locus_tag="ACG8PO_001790"
     CDS             5135..9487
                     /locus_tag="ACG8PO_001790"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HFQ0207099.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIQ
                     LGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSFIV
                     KKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYAPE
                     GEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGKKA
                     GVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAVKM
                     YKGNVIESFDEFIDPGHPLSRTTVDLTGITDEMVRGSKSEEEVLRMFLEFSKDTILVA
                     HNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSLEQ
                     HHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILAKN
                     QAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKGVE
                     EARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVATGN
                     VHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEIVV
                     ENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRLEK
                     ELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAPHY
                     YCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPDID
                     LNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSAEV
                     DRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHSIH
                     DNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIYSK
                     TGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDATLA
                     EVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVPDW
                     YIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMCKG
                     KDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFVIE
                     GDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGVLK
                     DLPDENQLSLFDML"
     gene            9631..10104
                     /gene="rimP"
                     /locus_tag="ACG8PO_001791"
     CDS             9631..10104
                     /gene="rimP"
                     /locus_tag="ACG8PO_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFQ0207100.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            10128..11303
                     /gene="nusA"
                     /locus_tag="ACG8PO_001792"
     CDS             10128..11303
                     /gene="nusA"
                     /locus_tag="ACG8PO_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFQ0207101.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            11325..11618
                     /locus_tag="ACG8PO_001793"
     CDS             11325..11618
                     /locus_tag="ACG8PO_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HFQ0207102.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            11615..11926
                     /locus_tag="ACG8PO_001794"
     CDS             11615..11926
                     /locus_tag="ACG8PO_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HFQ0207103.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            11939..14245
                     /gene="infB"
                     /locus_tag="ACG8PO_001795"
     CDS             11939..14245
                     /gene="infB"
                     /locus_tag="ACG8PO_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFQ0207104.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSSDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            14272..14619
                     /gene="rbfA"
                     /locus_tag="ACG8PO_001796"
     CDS             14272..14619
                     /gene="rbfA"
                     /locus_tag="ACG8PO_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFQ0207105.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKKAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(14727..15231)
                     /locus_tag="ACG8PO_001797"
                     /pseudo
     CDS             complement(14727..15231)
                     /locus_tag="ACG8PO_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035543.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            15396..17759
                     /locus_tag="ACG8PO_001798"
     CDS             15396..17759
                     /locus_tag="ACG8PO_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase"
                     /protein_id="HFQ0207106.1"
                     /translation="MDYSSKEYFDKMTAWWRAANYLSVGQLYLKDNPLLRRTLKPEDV
                     KKHPIGHWGTIPGQNFIYVHLNRVINKYDLNMFYIEGPGHGGQVMVSNAYLDGSYTEI
                     YPEVTEDETGMQKLFKRFSFPGGIASHAAPETPGSIHEGGELGYSLSHAVGAVLDNPE
                     VISAVVIGDGEAETGPLAGSWFSNVFINPVIDGAVLPILHLNGAKIANPTILARKSDG
                     ELANYFNGLGWEPFFIEGNDPEKLNPVMAEKMDQAIEKIKSIQKEARLKTATDVVMPK
                     WPVLIVRTPKGWTGPKEWDGEPIEGTFRAHQVPIPVDQEHMDHADALLRWLKSYEPEK
                     LFDAQGRILEEIREIAPTGDQRMAKNPITNGGIDPRPLIMPDWKKYTLQFEKPGSVKA
                     EDMTELGKFVREIIEKNPENFRIFGPDETKSNRLNQVFKTTNRQWMEKIEPENDEWLS
                     PSGRVIDSQLSEHQDEGFLEGYVLTGRHGFFASYESFLRVVDSMLTQHFKWIRKSHDL
                     SWRKDYPSLNLIASSTVFQQDHNGYSHQDPGILTHLAEKKAEFIREYLPADANTLLAV
                     MDKAFRSSEKINLIISSKHPRAQFYSAEEAAVLVNEGLKIIDWASTAKEEEPELVIAA
                     AGTESNLEALAAVTLLLEEFPKLKIRFINVVDLLKLRHPSQDPRGLSDEEFDQYFTKD
                     KPILFAFHGYETLVRTIFFDRHNHHLMIHGYKENGDITTPFDMRVVNELDRYHLAKDA
                     ALKIKGSQAEDFAEKMDQKLQEHQNYIRENGIDLPEVLDWKWKNLDQ"
BASE COUNT         6083 a         3154 c         3919 g         4663 t
ORIGIN      
        1 attattcagt ttttaaataa aaaaacaatg atttcatagg aaaaacagtt agatattgct
       61 ataatactta agatagtggg aatcttttaa gtataggagg aaaaagaatg ttacgttttt
      121 ttccgcaaaa aaacaaatat attcaagaaa aaagcgaatt tcaatttaat accgatggac
      181 caattcctca gcacattgcc atcatcatgg acggcaatgg tcgttgggcg caaaaccgtc
      241 gtttgccacg agttgctggc cataaagaag gcatggaaac agtaaagaaa gtcacaaaaa
      301 aagcatcaag gcttggagtg aaagtgttga cgctttatgc cttttcgact gaaaactgga
      361 agcgaccaaa agatgaagta agttttctca tgcaactacc tgtcgacttc tttgatacgt
      421 tcgtaccaga attgattaaa gaaaatgtca aagttcatgt catgggttat gaaaacgtat
      481 tgccagaaca tacgcaagat gctgtaagac gtgcaattga gcaaacaaaa aacaatactg
      541 gaatggtatt gaattttgct ttgaattatg gaagtagagc agagatcgtg actgctgtcc
      601 aagaaattgc cgaagaagta gcaaaaggag aaatacacgc agaagaaatc gatgatgaat
      661 tgattgctaa gcatttaatg acaggtttct tgcctaaaga gttacaagat cctgagctga
      721 tgatccgtac aagtggagaa gagcgaatta gtaattttct gttatggcag atcgcatata
      781 gtgagctgta ttttacaaaa gcgctttggc ctgattttga tggtgcgcat cttgaagaag
      841 caatcgcctc ttatcaaaat cgagaccgtc gattcggcgg tgtaaaaaaa acagaagatg
      901 agaaagggga tcagtcatga aacaacgtgt gatcacagca gctgtggcgc tagtcttgtt
      961 tattccgatt atatggatcg gaggcatagc ggttgagctt acagcagcaa tcttagcggt
     1021 agtaggagtt tacgagctgt tccggatgaa aggattgaca ctcttaagtt ttgaaggagt
     1081 tttatccgcc gttggtgcag tgttacttgt tttgccaaaa gaaagatggt ttttctttct
     1141 tccagagaat gtaagtacat ttatgttatt ttatcttata gttatgattc ttttaggaac
     1201 atcagttatt tctaaaaata cgtatacgat tgatgaagca ggttttcctg tgcttgtcag
     1261 cttgtatgtg ggaataggtt tccagaattt tgtcaatgca cgaacagaaa gcttggctgt
     1321 tttgatattt ggtctattta ttgtttgggc aactgatatc ggagcatata tggttggtcg
     1381 acgttatggt cagcataagt taagtcctga tatttcgccc aacaaaacga ttgaaggtgc
     1441 gctcggtggt atcggtagtg ctgttgtagt cgcatttctt tatttattgc tatacccagc
     1501 aaaagaacta ttcaatcatg gtatgtttgt catgcttctg atgacgatta ttttttcagc
     1561 agtcggacaa tttggtgatt tggtcgaatc tgcaatcaaa cgtcattatg atgtgaaaga
     1621 ctcgggaaat atcttaccag gccatggtgg aatcttagac cgtttcgaca gtttgctgtt
     1681 tgtatttcct atcatgcatt tatttggcat tatttagtaa gtatacctgc gtttctttgt
     1741 gaatttcata aaagacgcag gtttttattt ttcattatat tgatactatt ggtgtctttg
     1801 gtatactatg gtaaaattat agtgttttat cgttgtttta taaaaaacac aaaaaacaag
     1861 taaaggagaa aaaagctatg aaaacgattc tgacatttat catcgttttt ggtatattag
     1921 tgattgttca tgagtttggt catttcttct ttgcgaaacg atcaggaatc ctcgtccgcg
     1981 aatttgctat cggtatgggt ccgaaaattt atgggcatca agcaaaagac gggacaacat
     2041 atacgcttcg cctattacct attggcggtt atgttcgaat ggcaggcaat ggggatgatg
     2101 aaacagaaat ggcaccagga atgcctcttt ctcttttact aaattctgac ggcatcgtag
     2161 agaagataaa tttaagtaag aaaatccaat tgacaaatgc tatcccgatg gaattgagcc
     2221 gttatgattt agaagatgag ctgactatca ctggttatgt gaatggagac gaaacagaag
     2281 tagtaaccta ccctgttgat cacgatgcga cgattattga aaacgacgga acagaaatca
     2341 gaattgcgcc aaaagacgta cagttccaat cggctaaatt gtggcagcgt atgctgacca
     2401 actttgctgg acccatgaat aactttattt tggcaatcgt gttattcatc attctggcat
     2461 ttatgcaagg tggtgttcaa gtgacgaaca ctaatcgcgt aggagagatc atgcctaatg
     2521 gagctgcggc agaagcaggc ttaaaagaaa atgatgaggt agtcagtgtg gacggaaaag
     2581 aaatccattc atggaatgat ctgacaacag tcattacgaa aaatccaggt aaaacattag
     2641 actttaagat cgaacgagaa ggtcaggtgc aatctgtcga cgtgacaccg aaatctgtag
     2701 agtcaaatgg cgaaaaagtt ggccaacttg gtataaaagc cccaatgaat acaggtttta
     2761 tggataagat cattggaggg acgcgtcaag ctttcagcgg ttcattggaa attttcaaag
     2821 ccttaggatc actatttaca ggtttcagtt tagataaatt aggcggacct gtcatgatgt
     2881 accaattgtc ttcagaagca gcaaatcaag gtgtaacaac tgttattagt ttgatggcgc
     2941 ttctttcaat gaatctcgga atcgtcaatc tgcttccgat tcctgcctta gatggcggga
     3001 aattagtctt gaatattttt gaagggattc gcgggaaacc attaagtcaa gaaaaagaag
     3061 gaatattgac attagctggc tttggatttt taatgttgtt gatggtgcta gttacatgga
     3121 acgatattca acgattcttt ttctagaaaa agttgacaaa tagggtataa taacaaacga
     3181 taactatttt gaggaaataa aggagtatat agaatgagac aatcaaaaat gttgattcca
     3241 acattacgcg aagttccaaa tgatgcagaa gtattaagcc atcaaatctt gctaagagct
     3301 ggatatatcc gccaagtatc agcgggtatt tattcatatc tgccattagc aaatcgtgta
     3361 ttggaaaaac tgaaacgcat tatgcgggaa gaatttgaaa aaatcggagc ggtagaaatg
     3421 ctgatgcctg ccatcttgcc agcagaactt tgggaagaat ccgggcgtta cgaaacatat
     3481 ggaccaaact tgtatcgttt caaagaccgc aatgatcgaa aaatgatcct tggaccaact
     3541 cacgaagaaa cttttactga tttgatgcgt aatgaaatca attcatataa gaaattacct
     3601 ttaaatcttt atcaaatcca acctaaatat cgtgacgaaa aacgacctcg cttcggtttg
     3661 cttcgcggaa gagagtttat catgaaagat gcatattctt tccatgcaac ggaagaaagt
     3721 ttggacgaga catataaaga ttacgaaaaa gcctataccg aagtattcaa gcgttgtggc
     3781 ttagaatttc gctcgatcat cggagacggc ggcgcaatgg gcgggaaaga ctcaaaggaa
     3841 ttcatggcga tctcagaaat cggggaggat acgatctgtt attcgactga aagtgattat
     3901 gctgcaaact tggagatggc tacaagctac tatgtatcaa aaaaatcgca tgaaacacag
     3961 cttgaattgg agaaagtagc tacacctaac gtaaaatcaa ttgacgaagt agcagatttc
     4021 ttttctgttg aacctcaaaa aatcatcaag tctgtattgt tcattgcgga tgaacaacct
     4081 gtccttgttt tggtacgtgg agaccatgaa gtcaatgacg taaaactgaa aaacttttta
     4141 tctgctgact ttttagaaga agcaacagaa gaagaagcac aaaaatatct aggtgctgag
     4201 tttggttctg tcggaccagt cggtgtttca gatgacgtaa aagtatatgc agaccgttat
     4261 gtccaagatt tagcgaatgc cattactgga gcgaacgaaa caggattcca ctataaaaat
     4321 gtgaatccaa aacgcgattt cactgtgctt tcttatgaag atcttcgttt tgtacaagaa
     4381 ggagatcctt caccagacaa caacggtgtt cttgccttta ctcgaggaat cgaaatcgga
     4441 catatattca aaatcggcac tcgttacagt gaatctatgg gagcaactgt actagacgaa
     4501 aatggacgag aaaaatttgt gattatgggc tgttatggta tcggtgtaag tcgtttatta
     4561 tcagcaatcg tagaacaaca ttcagatgaa caaggaatca attggccaaa aggaattgcg
     4621 ccatttgatc tgcatatcgt ccaaatgaat ctaaaagacg aataccaaac aagcctgacg
     4681 aatgaactag aagaagcgat gacaaaagct ggctatgaag tactggttga tgacagaaat
     4741 gaacgcgcag gagtgaaatt cgcggattct gatttgattg gctgtccaat cagaatcact
     4801 gtcggtaaaa aagcagtgga tggaatcgta gaagtgaaaa tcaagaaaac tggcgaaatg
     4861 gttgaagtac gtaaagaaga attggcgaat actttaccaa tcctcttgaa ccaaggcgaa
     4921 taataagcgt taaaacacag aaaataaaaa tagctgaatc agctgttttt gacaatctga
     4981 tgattcgctt ttaaaattgg aactgcgatt tgaatcgcag ttccaatttt ttaatgtaaa
     5041 actcagaaaa aacgagtata ctaaaaggta ctgaaaaaga agaggataag atgcgatcaa
     5101 ttgcattgtt tctcatcaga aagggcgtat atctttgtca gaaaaacgag atttatttga
     5161 aaaactgttg gaacaaatcc aactagagga agcagaaaaa aatcatccac tgcttactgc
     5221 tggtgaaatg gatagtgtag tggtccatcg taaatcgcgt ttatgggaat ttacgctaca
     5281 tttttccaaa attttgccta ttatgctata tcggtcattg actcagcatc tgactatcgc
     5341 ttttaaagat attgcacaag tcaaactgaa tatcttagca gatgaccaga cattcgatga
     5401 acagctcttg caagattact gggcccaagc tttggaaaat cagcaatgcg atacaccact
     5461 agcacagcaa gtattgaaaa cgcaggtacc tgtgataaaa gatcgtaaag tcatcttgcc
     5521 aatcgacagt acaggagcta tttcttattt gaagcaacaa tacttgccat tagtagaaga
     5581 gttgttcgtc agttacggat tcccaaaatt tcgtatcgaa ccagaagtgg atgagcagca
     5641 agcggaacgt gtattgaaac tttttgaaga acgcaaacaa gaacaggcag aagcctttat
     5701 gaagcaagca gctgaaagtc tagttgtcca cgaacaaaag aaaaaggaaa gaaaagagca
     5761 atcgccagct cttgaaggac ccatccagct aggacgaaat attccagccg atgaaccaac
     5821 gacaccaatg atcagtatcg ttgaagaaga acgaagagtc acacttgagg ggtacgtatt
     5881 cgacaaagaa gtcagagaac tacgttcaaa acgcaagatc ttgactttga aaatcacaga
     5941 ttatacttca tcatttatcg tcaaaaaatt ctcgaacggc gaaaaagatg aacaagtatt
     6001 tgatgcgatc agtgtcggta gctggttaaa ggtacgagga agtattcaag aagatacgtt
     6061 tgtccgtgat ttggtcatga atgcgcaaga tatcatagaa gtcaaacata cacctcgcaa
     6121 agattatgct cctgaagggg aaaaacgagt agagcttcat gttcatagca atatgagtac
     6181 gatggatgca acaaacagca tctctgatct tgtggcacaa gcaggaaaat ggggacaccg
     6241 agctatcgcc attacagacc atggtggtgc gcaagccttt ccggaagcac atagtgctgg
     6301 aaaaaaagca ggtgtgaaaa tcttatacgg cgtggaagcg aatgtcgtag atgatggtgt
     6361 cccaattgct tataatgatg cacatgaagc attgagtgaa gccacatacg tcgtatttga
     6421 cgtggaaacg acaggattgt ctgcagtata tgacacgatc atcgagctag ccgcagtcaa
     6481 aatgtataaa ggaaatgtga tcgaaagctt tgatgaattt atcgatccag gacatccatt
     6541 gtcacgaaca acggtagatc tgactggtat cacggatgaa atggttcgag ggtcaaagtc
     6601 ggaagaagaa gtattgcgga tgttccttga attttcaaaa gatacaattt tggtagccca
     6661 caacgcggct tttgatatgg ggttcttgaa tactagctac gcaaggtatg gtattcctga
     6721 agcggccaat ccagtcattg atacgttgga attagctaga tatctgtatc ctcaattcaa
     6781 acgtttcgga ttaggtgtgt tatctaaaaa gtttggtgtt agcttggaac agcatcaccg
     6841 agcaatctat gatgctgaag caactggtca cttagcatgg atctttgtga aagaggcaat
     6901 ggacaatcat aacatgcttt atcatgatca gctaaatgag catatcggag aaggagattc
     6961 ttacaaaagg gctcgacctt tccatgtaac gattttagca aaaaatcagg caggactgaa
     7021 agatttgttc aaactgattt caatgtcgaa tgtcgaatac ttcgaacgcg tcccacgaat
     7081 accacgctcc caattgaaaa agatgcgtga aaatctattg atcggctcag cttgtgataa
     7141 gggagaaata ttcgaagcca tgatgcaaaa aggggtagaa gaggcaagaa accgagcaaa
     7201 attctatgat tatattgaag ttatgccaaa agcggtctat gcgcctttga tcgagcaaga
     7261 attggtaaaa aatgaacatg atttggaaga aattatccaa aatttggtag aaatcggaaa
     7321 aagtttggat aaaatcgttg ttgcaacagg taatgtacat tatctgaacg aagaagatgc
     7381 catttaccga aaaattttga tcaattctat gggcggagcg aatccgttga atcgtcatag
     7441 tttgccggat gtccatttcc gaacaaccga tgagatgcta actgcatttc actttttagg
     7501 ggaagaaaca gcgaaagaga tcgtagtaga aaatacgaat aaaatcgcag atatctgcga
     7561 agaagtgatt ccagtcaaag atgaacttta tacaccgaag attccaggtt cagaagatga
     7621 aatatcagaa ttgagttaca caaaagcaaa acaaatgtac ggagatccgt tacctgagat
     7681 cattcaaaaa cgattagaaa aagagttgaa ttctattaat ggtaatggct tctcggttat
     7741 ttatctgatc gcccaaaaac tagtgcataa aagtaatgaa gatggctatc tagtaggttc
     7801 acgtggctct gttggctcta gctttgttgc gacgatgaca ggaatcactg aagttaatcc
     7861 attagcccct cattattatt gtccagagtg ccaatattcc gagttttttg aagatggtac
     7921 gtatggttct ggatttgaca tgccagaaaa acaatgtcca aaatgtggtg cacgcttaaa
     7981 caaagacggt catgatattc catttgagac atttttagga ttccatggag acaaagtgcc
     8041 cgatattgat ttgaacttct caggtgatta tcaagcagaa gcccataact atacgaaagt
     8101 attgtttggc gaagactatg tttaccgtgc cggaacgatt ggtacagtcg ccgacaaaac
     8161 agcatatggc tatgtaaaag gatacgaaag ggacaataat ctgcagtttc gaagtgcaga
     8221 agtcgacaga ttagcaaaag gcgctactgg agtcaaacgg acaaccggcc agcatccagg
     8281 ggggatcatc gttattcccg attatatgga tgtctatgat tttacgccga tccaatatcc
     8341 agcagacgat caaaattcag aatggaaaac gactcatttt gatttccact cgattcacga
     8401 taatgtattg aaattagata ttctagggca tgatgaccca actgttattc ggatgcttca
     8461 agatttatca gggatcgacc cacaaacgat ccctacggat gacccggaag tgatgcggat
     8521 atttgccggt cctgaagtac ttggagtaag tcaggaacaa atttattcca aaacagggac
     8581 attaggtatt ccagaattcg gcactcgttt tgttcgaggg atgttagaag aaacgcatcc
     8641 tacaactttt gcagaactac tgcaaatttc cggactatct catggtacag acgtatggtt
     8701 aggaaatgcg gaagaattga ttcgacgagg agatgcgaca ctggcggaag taatcggctg
     8761 tcgtgatgat attatggttt atttgatcca tgctggatta gatagcggga tggcatttaa
     8821 aatcatggaa actgttcgta aaggacaatg gaacaagatc cctgatgaat tgcgagaaac
     8881 ctatctatcg gcgatgaaag aaaataatgt gcccgactgg tatatcgatt cttgttcaaa
     8941 aatcaaatat atgtttccga aagcccatgc tgcagcttac gtattgatgg ctcttcgtgt
     9001 cgcttatttc aaggtttatt tccctatttt gtattactgt gcatactttt ctgtacgtgc
     9061 agacgacttt gatctagtct ccatgtgcaa aggaaaagat gctgtgaaac aagcaatgaa
     9121 agaaatcacg gacaagggat tagatgcttc cgtaaaagaa aagaatcaac tgacagtgct
     9181 ggagttagcc aatgaaatgc tggaacgagg attcaaattt ggaatgattg atttgtataa
     9241 atcggatgcc gttaactttg ttattgaagg tgatacgttg atcgcaccat ttagagctgt
     9301 cccaagtttg ggaaccaacg tggcgaaaca aatcgttgaa gcaagaaaag acggtccatt
     9361 tttatccaaa gaagatcttg ccacccgcgg taaagtttca aaaacattga tcgaatatat
     9421 gaacgacaat ggcgtattga aagatctacc agatgaaaat caattatcgt tattcgatat
     9481 gctataagca tctgttttcc tgtctgcttg taattttaag cagagtatgg tatagttctt
     9541 ataggtaatg atactaactg tgagtgagcg gatttttcgc tcactctttt taatggaatt
     9601 acgcacttaa tttttaagga ggcgaaactt ttgagtagcg tcgttgaaac agtcacagaa
     9661 atggtgacac cgattttaga tgaacaaaat tttgaacttg tcgaagtcga atttgtaaaa
     9721 gaaggaaaaa gctggttctt acgagtattt attgacaaag aaggcggtat cgatatcgaa
     9781 gaatgcgcct ttgtcagtga gaaactgagt gaaaaacttg atgcagcaga tccagatccg
     9841 attcctcaag cttatttcct ggaggtatcc tccccgggag cagagcggcc attgaaaaaa
     9901 gaaagcgact atgaaaaggc acttggtgat tatgtccatg tctccttata ccaaccagta
     9961 gatggcgaaa aacaatatga aggatttctt caatcatttg atgctgaaca gcttacattg
    10021 aaaatacgca taaaaacacg agaaaaagaa ttgacttttg accgaaaaaa tattgcaaaa
    10081 gctcgcttag ctatccaatt ttaatcacgg agaggaatca gaaaaaaatg agtaaagaaa
    10141 tgttaaatgc gttagacgct ttagaagctg aaaaagggat ctcaaaagaa atcgtgatcg
    10201 atgctttaga agcagcattg gtttctgcat acaaacgtca ctatggccaa gcacaaaacg
    10261 tcgaagtgga atttgatcag aaaaaaggaa agatccatgt ctatgcggta aaagaagtaa
    10321 cggaagaagt gatggattca cagttggaag tctcattgaa agatgcactt ttaatcaatc
    10381 cagcatatga gatcggtgat acgatccgtt ttgaagtaac accaaaagat tttggacgga
    10441 tcgctgcgca aacggctaaa caagttattt tacagcgtgt acgtgaagca gaaagaacga
    10501 ttatttataa cgaattcagt gcttatgaaa aagacatcat gcaaggaatc gtcgaacgcc
    10561 aggataaacg ttatatttat gtcaatcttg gtaaaattga ggcagttctt tctaaacaag
    10621 atcaaatgcc aaatgaattt tatcaaccac atgatcgaat caaagtttat gtttcccgag
    10681 tagaaaatac gtctaaaggt ccgcaagtat ttgttagccg cagccatcca gacctattgc
    10741 gtcgcttatt tgaacaagaa gtaccagaag tctatgatgg aatcgttgaa attgtcagca
    10801 tcgcccgtga agctggcgat cgttcaaaag tggcagttcg ctcaaatgat ccaaatatcg
    10861 atgcagtcgg tacatgtgtc gggccaaagg gacaacgagt tcaagcaatc gtcaatgaat
    10921 taaaaggcga aaacatggat atcgtcgaat ggaatgaaga tccagctgta ttcatcgcaa
    10981 atgcattgaa tcctgcacaa gtcatggatg tgatcttcga tgagacaaat ccaaaggcct
    11041 gcacagttgt cgttccagac taccaattat cgctagcaat cggtaaacgc ggacaaaatg
    11101 cacgtttagc tgctaaattg actaaccata aaatcgatat caaatcagaa tctgacatga
    11161 cggaatttta tgaaaatcaa gaacaacaaa aagaaacaga agaactgcat gatgaagcaa
    11221 tcgttcagtc agatttaaca gacgatgaat atgaaacaat tgcattcaac aatgaaacgg
    11281 tcgaagaaaa accagaagct taactttagt aaggaggcaa agagatgaaa caaaggaaaa
    11341 ttcctttacg caaatccgtg gtttcaggtg aaatgaaacc taaaaaagag atggtccgaa
    11401 tcacccgttc aaaagaaggg gaagtatcga tcgatccaac tggaaaaatg cctggacgag
    11461 gagcgtatgt ctcacttgag ccggaagaag tacaacaagc ttgggataaa aaaattctcg
    11521 atcgagtact cgaaacaaca ttaacagatg agttttatca agaacttctt gactatgtca
    11581 ctcaccaaaa agcacgtaaa gagttatttg gaaaatgaat acagttaatc ggcaaaaagc
    11641 catgaatctc atcggtctag cgatgcgcgc gggaaaacta atcacgggtg aagaactgac
    11701 cattgctgaa atccgcaaac aaaaagcaaa attggtgttt gtggcaaccg atgcaagtga
    11761 gaatacaaga aaaaagataa aagataagag ttcatattat gaagttcctt gctttgagct
    11821 attttctgaa gcagaaatca cgcagatgat cggaaaacct cgcaaggtat tcggaataat
    11881 ggatgccgga tttgcgaaga agaccaagga gctaattgaa ggttaggaag gtgattgcat
    11941 gggcaacaaa agaatctatg aattagcaaa agaactcaaa aaatctagta aagacgtcgt
    12001 ggaaaaagca caacaacttg gcatcgatgt gaaaaaccat atgggtgcga ttactagcag
    12061 tgatgagaag aaattgcaac aagcgttcaa aaacccaagc acgaaacaac aagctgcaca
    12121 acaagcaagc agaaaaccag ccaatcaaca gccgaacgat caacgtaaaa aagaaacaac
    12181 gaacaatcaa caaaaaaata aaaataaccg cagttatcaa gatcgcggtc aagggagcgg
    12241 tcaagtgaat caaggaaaaa aaccaacaac aaaccaaaat aatacgggaa ataccagcag
    12301 ccaaaatcgt caaggaacga gccaaggcgg caatcaaaac cgtactaaca ataacaacaa
    12361 accaaacaat aacaatcgcg gcaaatttaa taataataac cgtaatcgtt ataataaaaa
    12421 aggtaaaaaa ggcaaacagc aacaatcaac taaaccagct gtaccaccac gcaaattccg
    12481 tgaattgcca gatgtattag agtatactga aggtatgaac gtagcagata ttgcgaaaaa
    12541 aattcatcgt gagccagcag aaatcattaa aaaattattt atgcttggtg tcatggtcaa
    12601 tcaaaaccaa gcactggata aagatacgat tgaactgtta gcgacggact atggcatgga
    12661 accacaagaa aaagtccaag ttgacattgc tgatatcgat aaattcttcg aggcagaaga
    12721 agtcgatcca gataaattag tctcacgtcc gccagtagta acgattatgg gacacgtaga
    12781 ccacgggaaa acaaccttat tggatacatt gcgccattca cgtgtaacaa gcggcgaagc
    12841 cggcggaatc acacagcata tcggtgccta ccagatcgat attgatggta aaccaatcac
    12901 tttcttggat acaccaggac atgcggcctt tacaagtatg cgtgcacgtg gagcaagtat
    12961 cacagatatc acgatcttag ttgttgctgc agatgatggc gtcatgccac aaacagttga
    13021 agcaatcaac catgccaaag ctgcaggcgt tccaatcatc gtcgctgtaa ataaaattga
    13081 taaaccaggt gcaaatcctc agcatgttat gcaagaatta agtgagtatg aattaattcc
    13141 tgaagcatgg ggtggagaaa cgatttttgt tgagatctca gcgaaattcg gccaaaacat
    13201 cgaagaattg ctggaaatga tcctgttagt cgcagaagta gaagatttaa aagcagatcc
    13261 aacacaacgc gcaatcggaa cagtgatcga agcacgtctt gataaaggta aaggtcctgt
    13321 gtcaacctta cttgtccaag aaggaacact tcacgttggt gacccaatcg ttgtcggtaa
    13381 tacttacgga cgtgtccgtg taatgacaaa cgatcttggc cgtcgcgata aagaagcagg
    13441 acctgcaacg ccagtagaaa tcactggctt gaatgatgta cctcaagcag gtgaccgttt
    13501 cgtagtcttc gaagacgaaa agacagcacg tgcagcgggt gaagaacgcg caaaacgtgc
    13561 attattggaa aaccgtgctg ccacaagccg tgtgactttg gacaacttat ttgaaagcct
    13621 gaaagaaggc gaattgaaag aagttaacgt aatcatcaaa gcagatgtac aaggttcagc
    13681 agaagcactg gctgcatcat tgaagaagat tgatgtggaa ggtgtccgtg taaaaatcgt
    13741 ccattcagct gtcggagcaa tcaatgaaag tgatgtaacc ttagcagcag ccagcaacgc
    13801 tattatcatc ggtttcaacg ttcgtcctac accgcaggca aaagtgcaag ctgatactga
    13861 agaagtcgat attcgtcttc accgtatcat ctataaagcc atcgaagaaa tcgaaactgc
    13921 gatgaaaggg atgctagacc ctgaatttga agaaaaaatc accggtcaaa tgatcgttcg
    13981 tgaaaccttc aaagtttcta aagttggtac gattgcaggt gcctatgtaa cagaaggata
    14041 tattcgtaga gatagcggtg tccgtgtaat ccgtgacgga atcgtaatct atgaaggcca
    14101 acttgcaagt ctgaaacgat tcaaagatga cgtgaaagaa gtcaaaatgg gttatgaatg
    14161 tggtgcgatg attgaaaaat tcaatgacat caaagtagat gacgtaattg aaggatttat
    14221 catggaagaa gtcaaaaatg actaaaaact gataaaagga ggccaatcat tatggctaac
    14281 taccgtgatc gccgagtcgg tcaagaaatc ttaaaagaag tcaatgatat tttgcgcaaa
    14341 aaggtccgtg accctcgtgt ggagaacgta acgatcacag atgtgaaagt tacgggggac
    14401 ttacagcaag caacgatcta ttacagcatt ttatctgact tagcatcaga taagaaaaaa
    14461 gcacaagatg gattgaataa agcatcagga ttgattcgtc gagagttagg gcacaatcta
    14521 agtatttata agacacctga attgttcttt gaacttgatg aatcagttgt ctatgggaac
    14581 catatcgatg aattgcttcg cgacttgaac aaagactaat caaaaaatcc cgcaaatcct
    14641 gtgaaatcag gggttgcggg atttttttgt tttcattaag acgtattacc taagagttcc
    14701 atgactttag tctggtagcg tgtcagctaa ccaaaaaaga gaaaaagaaa agtaaaaaat
    14761 tcgactttct tttttctata gtatataaat ttttgcatat agataaagat caagataccg
    14821 ataacggaag ctagattgta atctatttta gtgacagtca ggtatagcca agtaactaga
    14881 agatcagtgg aacctgcacc ataaaagttt gagtttgtcg gtccgttgac agttaaaaga
    14941 aaaatcacgt tgaagttatt gatatttccg attacgccgc ttcgatttgg tcacgatcca
    15001 aattttggat aatccctgta gaaattagca tagtagctgg gataccaatt cacatattga
    15061 caaagataac agagactttt acataaaacg gatcaatcag gaaaggaatt gctcgatcca
    15121 gccccagttt tccaacaatg tgttaactgg cctagcgtca ttcagcaaat tatgcatgat
    15181 gaggagcgaa atgaactgcg gaacagccat tgtgataacg acaagttttt tggatcaatg
    15241 taaaaaacgt cagcgaaagg aaaaatttcg tggtttttta tacctgttca tttggctaga
    15301 tataaaatcc aaacaaatga acagaagtgc ttattaggtt tcaattggga gtatctgcgc
    15361 tagaataaga gtaataatga aaaggagtgt tatgtatgga ttattctagt aaagaatact
    15421 ttgataaaat gacggcttgg tggcgtgctg ctaattatct ttctgttggg caactttatc
    15481 ttaaagataa ccccttactg agacgtacac tgaaaccaga agatgtcaaa aaacatccaa
    15541 tcggtcattg gggaacgatt cctggccaaa attttattta tgtccatttg aaccgagtca
    15601 tcaataaata tgatttgaac atgttttata tcgaagggcc aggacacggc ggacaagtca
    15661 tggtatcgaa tgcttattta gatggaagtt acacagagat ctatccagaa gtaacggaag
    15721 atgagacagg aatgcaaaaa ttattcaagc gattttcatt tcctggaggc attgcttctc
    15781 atgcagcacc agaaacacca ggttctatcc atgaaggcgg agagcttgga tactctcttt
    15841 cacatgcagt cggagctgta ttagacaatc ctgaagtaat ctctgctgtc gtgattggtg
    15901 atggtgaagc agaaaccggt cctttagctg gaagctggtt ctcaaatgtc tttatcaatc
    15961 cagtgataga tggagctgtt ttaccgatcc ttcatttaaa tggtgcaaaa atagccaacc
    16021 caacgatctt ggcacgtaaa tcagatggag aattagcaaa ttactttaac ggacttggct
    16081 gggagccttt ctttattgaa ggaaatgatc cagaaaaatt aaatcctgtt atggctgaaa
    16141 aaatggatca agcaatcgag aaaatcaaat ccatccaaaa agaagctcgc ctgaagacag
    16201 caacagatgt cgtgatgcca aaatggccag tattgatcgt acgcacacca aaaggatgga
    16261 ccggtccaaa agaatgggac ggggaaccaa ttgaaggaac cttccgagca caccaagtac
    16321 cgattcctgt agatcaagaa catatggatc atgcagatgc tttactccgt tggctgaaat
    16381 cctatgaacc agaaaaatta tttgatgcac aaggacgtat tttagaagaa atcagagaaa
    16441 ttgcgcctac tggtgaccaa agaatggcaa aaaatcctat caccaatggt ggaatcgatc
    16501 caagaccgct tattatgcca gattggaaaa aatatacttt gcaatttgaa aaaccaggct
    16561 ccgttaaagc agaagatatg accgaacttg gtaaattcgt tcgcgagatc attgagaaaa
    16621 atccagaaaa tttccgtata tttggaccag atgaaacaaa atcaaaccga ttgaatcaag
    16681 ttttcaaaac gacaaatcgc caatggatgg aaaaaatcga accagaaaat gatgaatggc
    16741 tttcgccatc tggtcgcgtc atcgacagtc aattatcgga acaccaagat gaagggttct
    16801 tagaagggta cgttctgact ggacggcatg gtttttttgc aagttacgaa tcattcttgc
    16861 gagtggttga ctctatgttg actcagcact tcaaatggat acggaaatca catgaccttt
    16921 cttggcgaaa agattatcct tcgctgaatc tcattgcaag ctcaacagtc ttccagcaag
    16981 atcacaatgg atatagccac caagatccag gaatattgac acatttggca gaaaagaagg
    17041 cggagttcat ccgtgaatat ctgcctgcgg atgcaaatac attgctcgca gtcatggata
    17101 aagcattccg ctcgtcagaa aaaatcaatt tgatcatctc ttccaaacat ccgcgtgctc
    17161 aattttactc agcagaagaa gcagccgtct tagtgaatga aggcttgaaa atcattgatt
    17221 gggcttcgac agcaaaagag gaggaacctg aattggtcat tgctgctgca ggaacggaat
    17281 ctaacttaga agcattagca gctgtaactt tgctgctgga agaattccca aaactaaaaa
    17341 ttcggttcat caatgtcgta gatttattga aattacgtca tccaagtcaa gatccgcgtg
    17401 ggttgagcga tgaagaattt gatcaatatt ttacaaaaga caaaccgatc ttatttgctt
    17461 tccacggtta tgaaacattg gtccgcacga ttttctttga tcgtcataac catcatttaa
    17521 tgattcatgg ctacaaagaa aatggagata tcacgacacc atttgacatg cgtgtagtaa
    17581 atgaacttga tcgttatcat ttggcaaaag acgcagcttt aaaaatcaaa ggaagtcaag
    17641 cagaagattt tgcggaaaaa atggatcaga aattacaaga acatcaaaac tatattcgtg
    17701 aaaatggtat tgatctgcca gaagtattgg attggaaatg gaaaaatctt gaccaataaa
    17761 atgataaaat cgatcccgca aaacagaatt ttttacattg tattatttgt caaattaag
//
LOCUS       DAZKKW010000055        17636 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.056, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000055 DAZKKW010000000
VERSION     DAZKKW010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17636
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.056"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(116..1696)
                     /locus_tag="ACG8PO_001799"
     CDS             complement(116..1696)
                     /locus_tag="ACG8PO_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFQ0207107.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            complement(2097..3473)
                     /locus_tag="ACG8PO_001800"
     CDS             complement(2097..3473)
                     /locus_tag="ACG8PO_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFQ0207108.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(3601..4689)
                     /locus_tag="ACG8PO_001801"
     CDS             complement(3601..4689)
                     /locus_tag="ACG8PO_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFQ0207109.1"
                     /translation="MFWSLELLIIAALIMISSEINFIFEPIGTFFSTLFAPVLIAGFL
                     YYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQISSLASNMPDF
                     IKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSLGSIVGTIASA
                     TVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTLANYISGQAIE
                     CLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLVTVFNDPVKAL
                     LCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLGIFLGVPFYAI
                     CKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            4861..5295
                     /locus_tag="ACG8PO_001802"
     CDS             4861..5295
                     /locus_tag="ACG8PO_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0207110.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(5373..5906)
                     /locus_tag="ACG8PO_001803"
     CDS             complement(5373..5906)
                     /locus_tag="ACG8PO_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HFQ0207111.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(6000..7178)
                     /gene="mutY"
                     /locus_tag="ACG8PO_001804"
     CDS             complement(6000..7178)
                     /gene="mutY"
                     /locus_tag="ACG8PO_001804"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFQ0207112.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(7171..7968)
                     /gene="recX"
                     /locus_tag="ACG8PO_001805"
     CDS             complement(7171..7968)
                     /gene="recX"
                     /locus_tag="ACG8PO_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFQ0207113.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            8075..9457
                     /gene="rlmD"
                     /locus_tag="ACG8PO_001806"
     CDS             8075..9457
                     /gene="rlmD"
                     /locus_tag="ACG8PO_001806"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFQ0207114.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(9631..10209)
                     /locus_tag="ACG8PO_001807"
     CDS             complement(9631..10209)
                     /locus_tag="ACG8PO_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic polysaccharide monooxygenase"
                     /protein_id="HFQ0207115.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            10531..11001
                     /locus_tag="ACG8PO_001808"
     CDS             10531..11001
                     /locus_tag="ACG8PO_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TspO/MBR family protein"
                     /protein_id="HFQ0207116.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(11108..12334)
                     /locus_tag="ACG8PO_001809"
     CDS             complement(11108..12334)
                     /locus_tag="ACG8PO_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFQ0207117.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(13088..13288)
                     /locus_tag="ACG8PO_001810"
     CDS             complement(13088..13288)
                     /locus_tag="ACG8PO_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFQ0207118.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(13422..14030)
                     /locus_tag="ACG8PO_001811"
     CDS             complement(13422..14030)
                     /locus_tag="ACG8PO_001811"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HFQ0207119.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(14115..14585)
                     /locus_tag="ACG8PO_001812"
     CDS             complement(14115..14585)
                     /locus_tag="ACG8PO_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HFQ0207120.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            complement(14633..15547)
                     /locus_tag="ACG8PO_001813"
     CDS             complement(14633..15547)
                     /locus_tag="ACG8PO_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFQ0207121.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            15693..16496
                     /locus_tag="ACG8PO_001814"
     CDS             15693..16496
                     /locus_tag="ACG8PO_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HFQ0207122.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            16674..17621
                     /locus_tag="ACG8PO_001815"
     CDS             16674..17621
                     /locus_tag="ACG8PO_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFQ0207123.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
BASE COUNT         5334 a         3538 c         2908 g         5856 t
ORIGIN      
        1 atagtaaaaa gagccgtcac ggaaaatccg tcacggctct aaacaaaata tgagaggctg
       61 tttttgtttg gctattctta ttacttgatg cagggcagct tgtgtcaaga cttctttaca
      121 tcaaactttt taattttaca ttgggatatt tatccgcaaa ccagcgttct gcaaattggt
      181 tctcgaataa gaacaacggt tggtcgaaac ggtcacgagc caagatattg cgacttgaac
      241 tcatcttctc atccaaatct tctggatcga tccagcgagc gattttgtgt cccattggag
      301 acatgattac ttcggcgtta tactcattta acatacgata ttggaatact tcgaattgta
      361 gctgaccaac agctccaatg atatattctt ccgttagata tgttttataa agctgaatcg
      421 ctccttcttg aactaattga tagattcctt tgtggaagga tttttgtttc atgacatttt
      481 ttgcagtcac tttcataaac agctctggag taaatgaagg gagttcttca taagcaactt
      541 ttagttttcc ttcgaacaat gtatccccaa tctgataatt gcctgtatca tatacaccga
      601 tgatatcgcc tgctactgct ttttcgacgt tttctctcga atctgccata aattgtgtga
      661 cattgcttaa tttcagtttt ttatttgtac gttccaacca gacatccatt cctcgttcga
      721 atgttccaga acagattcgg acaaaagcga ttcggtcacg atgggctgga ttcatatttg
      781 cttggatctt gaatacgaaa cctgaaaatt cttcctcata aggacttacc atctgatcat
      841 cttgtgtttt gtgtccataa ggagctggtg ccaattcaac gaaagtctct aaaaaagtct
      901 gtacaccgaa gttagtcaat gcagaaccaa aaaatactgg tgtctgatcg ccacgagcaa
      961 ttttttcttg attaaatgca tctccagcct ctttgattag ttctacttct tctaagactt
     1021 gttcatataa ggaattttca gttagaggat gattttcggg aagcagtcca tcttcatcca
     1081 attctactaa tcgttcatcc tgataatttt ccggacgata aaactctaca cgattatggt
     1141 agatatcata aagaccttct agccctttac ccataccgat tggccaattc atcggataag
     1201 attcgatatc taatagctct tctaattctt ctaacaattc caatggttca cgaccatcac
     1261 gatctagttt gttgataaaa gtaaatatcg ggatgccacg tttttttaca acttggaata
     1321 atttctttgt ctgtgcttcg atcccttttg cactatcgat taccatgacc gcactatcca
     1381 cggccatcaa agtacgataa gtatcttcag aaaaatcttc atgtcctggt gtatccaaga
     1441 tatttactcg tttcccttga taatcaaact gcataacaga actagtaaca gaaatcccac
     1501 gttgtttctc gatttccatc cagtctgatt tggcaaaatt acctgttttt ttgcctttta
     1561 cggtcccggc ttgacggata gccccaccaa acaatagtaa ttgttctgta atcgttgttt
     1621 tcccagcatc cggatgggaa atgatcgcaa aagttctgcg attgtccact tgttgcttta
     1681 attttggatt attcataacc taactccatt tcttccattc aattatttat attttctcta
     1741 ggcacagatt gtccgatctc ttaaagaaag acaatttcat atagggctgt gacaaaagcc
     1801 gtgtagcagt ctctttttcc gagtaaacgg cgttctagag acagcctatc agcaataagc
     1861 ccacccaagc caaaaatacg aaaagctgtt tttgcttggc tgctcttatt acttaatgca
     1921 ggatagcttt ttcacaacct ttgtcaacta ctacaactga atcagtatag catggattca
     1981 gcgaatgaaa aagccaccaa accataagag tcaaatattt ttttgtcagc tgactaaaag
     2041 aaaagacgtg ttgcttgttt attcaagcaa cacgtctttc ataaccaagt ttccaattat
     2101 cttcttggct cgtcatcttc gaattctttt ctgaagtatc ttcgttcttc ttcaggttcc
     2161 gcttctgttt cttccggatc atgaaaaatg acacggattt taaggattcg agaaccttcc
     2221 atttcttcag aaaccagcgt aatattatcg acatcaaacg ataacttttc gccttcatcc
     2281 ggaatcatgc ctaatgctgt aatcaaataa ccagccatcg tatctacatc actcatatgc
     2341 agatcactgt caaaagcttc attgaactca tcaatcagca tacgtccttg gatgatatat
     2401 tcgtgttcat cgattttttc ataaagattc tcaacttcat ctgattcatc atcgatttct
     2461 ccgacgattt cttctaaaag gtcttctagt gttgctaacc caacgacgcc gccatattca
     2521 tctaataaaa tagccatctg attctgcgtc tttttcattt catataacag atcatcaata
     2581 aagaccgttt caggaacgaa taaaggctcc tgcatgattt tcttgatatc taaattatca
     2641 aagccgaatt tatgggcagc ttttagtaaa ttctttgtat gaaggattcc gactacttta
     2701 tctttgtctt cgttataaac agggattctt gaatagtttt cggataaaac ttcattcaca
     2761 ttttcttcca cagtgtcgtt gatatcaatc ataaatgcat ccgttcgtgg gaccatgact
     2821 tctcgggcaa ctttcgtatc aagagaaaaa accccttgga gcatttctaa ttcttcatta
     2881 ttcaatacgc cttcgttttc aagcatataa cgcatttcat ctctggtcat ttttgaatct
     2941 tcatcatcaa aagtcatcgg cgtgatacgc gccaataaac ttgtggaagc agaaagcagc
     3001 cagacgaagg gcttagctac tacaccaatc aagcgtatca gccctgaagt gaactttgcg
     3061 acttcttctg atttatttaa agcaatacgt ttagggtaca attctccaaa aacaatcgat
     3121 acataggtca aaatagccag cacaatgata tttgcaatat tttttgccgc tgtgccgcca
     3181 cctaataccg gagctaaccg ttccgataaa gtattagcta gtgaagcacc agacaaaatg
     3241 ttgaccaaag tgattcctac ttggattgtc gataggaaat tatttggatt ctgcaatatt
     3301 tttaaaagct tttgagattt cacatctcct tcatcagctt tttgctcgat tcgattctta
     3361 ttgatcgaaa caacagcaat ttcggaagct gctaaaaaag cattgatcaa tgtcaaaacg
     3421 atcaataata aaatctgcgc taacagcgac tgactctcag ggtcagcatt cataatcgca
     3481 ttctctcctt ataaaattaa tttttagttt acgatagaaa aaaacgtggc actatagcca
     3541 cgttaaaatg gtatcacaaa gcaattttct ttgcaataaa aacaagtgta ctaaaactac
     3601 ctacgaggtc gtagcaattt tcttcttgtt ttcattttgc ttgtcttctt tgacgatttt
     3661 gattatatag gaaatgatcg tcttgcagat tgcgtaaaaa ggaacaccta agaagatacc
     3721 aagcaatccg gcaatatttc cagctactag aagaacgata atgatcgtta atggatggat
     3781 tttcagtgat ttcccaatta cattaggata aatgatattc ccatccaact gttggacgat
     3841 tagcacaacc acacaacaaa gtaatgcttt taccggatca ttgaagactg ttactagaaa
     3901 tgcaggtgct agtcctagat aaggtcctaa atatggaatt aggtttgtca atcccgcaat
     3961 gaccccaaat aagaaagcat aatccacacc aattaccata tatcctaaaa atgtgaaggt
     4021 accaacgaat aaacattcga ttgcttgtcc gctaatgtaa ttagccaatg ttttattcag
     4081 ttgccccaat aactccacaa tttggtttct tcttttttct gggaaaaact gttggatgtt
     4141 tggcactagt ttttctccgt ctttcaacat atagaacaag atgaatggtg ctgtaatgat
     4201 tactacggtt gccgaagcga tcgttccaac gatcgaacct aaactattgg atactccact
     4261 aaggaattgc tgaatgattg tcccgtaaga aatgtctaat tgctcaaaat atttattgat
     4321 atctacttcc ttgaacaaag gcaattcggc tacctctttt aaccagcttt ctacctgttt
     4381 gatgaaatca ggcatattag atgccaatga ggaaatttgc tgtactagac ttgggatgac
     4441 actaagtaaa atccaaataa ttgctgcaac taaaaggagc agcacaattg cgacagccca
     4501 tattcttttg atttttgctt tcatcaacag cttgacaata ggattcaata aatagtaaag
     4561 aaagcctgca atcagtacag gtgcaaataa cgtggaaaaa aatgtcccga tcggttcgaa
     4621 aataaagttt atttctgatg aaatcatgat caatgcagct atgatcaata attcaagcga
     4681 ccaaaacatt agtttagact gtctaagttt ttcaaacaat agatttcctc ctttctttct
     4741 tactagtata tattatattc gttaaaaatt cttttattct ttttttattt ttgaaaactt
     4801 gccatacttt ttatttgttt tattttccag tatactatac gaaaggaagg tggctagacc
     4861 atgaaagttg tacaaacaaa agatacaatg agcaatattt acttagatgc tgtacggatc
     4921 aggcatcaag tttttgtagt cgaacagggt gtgcctttgt ctcgagaaat agacaaagat
     4981 gaagcccatt gtatccactt cgtcctgtac tcagataaaa aagaaccgca aggaactgta
     5041 cgtcttttgc ctttagaaaa cggaaaaatg aaattacagc ggatggcgat tctttctgaa
     5101 tacagacacc aagggttagg aaaaatattg attgaagaag cagaaaactt tgctaagaat
     5161 caaggctata atacgatttt attaggggca cagtccacag ctgaaacttt ttacgaaaaa
     5221 ctaggatata cagcttacgg cgatcctttt gaagatgctg gcatgcctca tatttatatg
     5281 aaaaagactt tataaaaatt tatttcgaaa aaagcaaaaa acagcaagct tttgagaaaa
     5341 atctctcact ggcttactgt tcttttttgt gtttacttac gcgacaattg cttgtaacgg
     5401 ttataccaaa tatcaatata gccttccgag aacgggccat ctccattatt gatccagtca
     5461 accagaatct tcacattttc ttttaggatg aagtcaatat cgttcgggta attcatcatt
     5521 ttgctgtgca tctcatattc atcgacatcc aatagtcgtt tctcgccgtc aggaaatacc
     5581 ttgatatcca agtcatagtc gatatatttc aaggcctcat cgtccagcaa aaaaggtgaa
     5641 gctagattac aataatagga aacccccttt tctctgatca ttgctattac attgaaccag
     5701 tattttttat gaaaataaac aatcgctggt tcgcgcgtta cccatcggcg tccatcagat
     5761 tcagtgacta atgtgtgatc attcacgccg atcatagagt gttcgcttgt ttttaatacc
     5821 atggtatctc gccacgttcg atgcaatttg ccgtcgtgct tgtaactttg gatcgtcaca
     5881 aactctcctt cttttggaac tcccatagca acccttcctt aagcagcatt ttgtgctttg
     5941 tgtttgggtg ttcattacac cgacatcata attattatat caataaaaat gagctttgcc
     6001 tagaaaggat tcctattgtt tcgatttttc tctaactgat ctaccagttt catttggacc
     6061 ttaggaaaaa cgaccgaatc aaatgcatca ggcttcaacc acttcgaagt tttgttctcg
     6121 gtaaattctt gttctgctcc ctcggttgcc cgtccgtaaa aaaggagaac atgccatttc
     6181 agatgactaa aaatatgagt aacttctcct aaatgtcgtg tctgccaaac aaagagttcc
     6241 tgcttttcaa atgggagatt ttgctcttct tctgctacta ggtcatcaaa cagatcgatt
     6301 tctggctttg tttcccattc ttttttcaat cgctcatact cctcttgtgt cacttccatc
     6361 attggaaatg tccacatatt cgccagcaat ttttggctat ctctttcttc aaaataatac
     6421 gctccagaat gattttgaag agctgcactg atataataaa cgtcttttgg ctttgctttt
     6481 tttgttttta caggaaaact cgtttggatt cctcgtttgt tagctaaaca aaaggcttgg
     6541 atcggacaag tctcacactt tggagaagtt ggtgtacaga tagctgaacc tagatccatc
     6601 attgcttggt tgaattcacc tggatgcttt tcatcaatga ttttccgcat cgcttcatca
     6661 aagattttcc tgctagaagc ttttgcgatg tctgcttcaa tgcaaaacaa tctgctgact
     6721 actcgcatca cgttgccgtc aactgcaggt tctggaagtc caaatgcgat acttgcaatc
     6781 gcgcctgtcg tatatggtcc tattcccttc aatgagctaa tttcttcagg cgtttgcggc
     6841 atttccccat caaattctga catgatctgt ttagcagcag cttggatgtt ccgggctctt
     6901 gagtaataac caaggccctc ccatgctttt aaaagctttt cttctggagc atttgctagc
     6961 tcttcaattg taggaaacca ttccatgaaa cgataaaaat aatcgatcac tgtgtctact
     7021 ctggtttgct gaagcatgat ctcagagatc cagatacgat aaggatcacg attatagcgc
     7081 caaggcaggt ttcttttttc ttgttcatac cattgaatga actgatcttg aaattctttt
     7141 gtttctttat ctgtccattt ttcccattct ttattcactt tcctcttcct tttccgtaat
     7201 gaatcgctgg atcatatcca aatcaaatcc tttttgatat aagctttgct ttacttttag
     7261 atttcttttt ttaggttcaa accttaaatt tcttcgccat agtttatctc cttgtttgac
     7321 taaataatca tattctattt cttgatctgc ttcttgcgga agatcagcta tcgcttgttg
     7381 gatcacttct tgagaaaagc ctttcgtcat caatgtttgt tggatttttc tcaataattc
     7441 tttttggctt tttccgtgat tttttccaaa cgctttttcg gctgtctgat gtgcattttg
     7501 aacttggtct tcaaatgtat attggctgag cgcttgttca atgatttcag gtttcaaacc
     7561 tttttgctgc atctgctgcg ctaatttctt tggtcctttg tcacttaagc gcatattcgt
     7621 gcgcacataa ctttccccat agaccaaatc atcgacaagg tccaattctt ttaagcgggc
     7681 tacgatttta tttcgatctt cttgaaggat ttccttttct tttaagtatg ttcggatttc
     7741 tttttccgat cgcaactgat aacttaagta attcattgct aggtgaaaac cgaaatcagc
     7801 tccgctactc ttcttgatct cttggatagt ttcttcgtct aattcccgtc cttttaataa
     7861 tcgatagcgt actaatacat cttcagatac ttgtaaagct tctccttcag ataaatctac
     7921 gcgatagaaa gggcctttgc ctttactgat tctcgtaacg gttaacataa acatcctcct
     7981 cgaacatata ttctctatca taccacagaa aatccccgta atcaggtatt atatgttaga
     8041 ataaaccaca gaaaaaagaa aaagggagat acttatggca gaaacaattg tcaaaactgg
     8101 acaaacggtt cctttgaaaa tcaagcgttt aggcataaat ggtgaaggaa tcggttattt
     8161 caaacggttg atcatatttg ttccatatgc actaccaaaa gaagaagtac tagtgaaaat
     8221 cacgaaagca actcctcgct atgcagaagg agaattaata aaagtcaaaa aaacaagcaa
     8281 agatcgcgta gtggcaccat gtcctgttta ctatgaatgc ggtggctgtc aattacaaca
     8341 cttagcttat caagctcaat tggattttaa aaaggatctg cttttgcaag cattggaaaa
     8401 attcaaacca gcaggctttc gatcttatca acttttgccg acgatcggga tggatgagcc
     8461 ttggcattat agaaacaaag cacaatttca gttacgaaaa aataagcaga ctcaaaaaat
     8521 tgaagctggt ttatacgaag caaactctca tcagttagtc cctataacag actgcttggt
     8581 tcaagaacct cgaacacaaa aagtaatgaa tactgttgtt caactattga ataaatttga
     8641 tgcacccatc tatgatgaac ggacaaacag tggaatcttc cgcacaatca tggttagaat
     8701 cggtgttaaa actggggagt tacaagttgt ttttattact cgcagtaaaa aattcccgca
     8761 aaaaaacgca atgatccgtg agatcaatga acgactgccc gaagttgttt caatcatgca
     8821 aaatgtccag gcacaaaaaa cgtctctcgt gatgggggat gaaacgatcc acttatgggg
     8881 aaaagaagcc attgaagaac aaatcaatga agtgactttc gatctttctc ctcgtgcctt
     8941 ctttcaactc aatcctcagc agaccgaatt gctttatggc gaaggaatca aagcattaga
     9001 tgtccagcct aatgaaacga tcgtggatgc ttattgcgga gttgggacga tcggcttaag
     9061 tgtagcaaaa caagccaaag aagtacgggg aatggatacc gttcctcaag caatcgctga
     9121 tgcaaaaaaa aatgcggaaa gattaggata tcacaacact cgatatgaag tcgggacagc
     9181 agagcaagtt ctgcctaaat ggttgaatga aggttttcag cctgatggta tcatcgtaga
     9241 tccaccacgt acgggtttgg ataacgcatt gatccaatca ttattgaaat accctccaaa
     9301 aaaacttgtt tatatctcct gtaatgtttc tacacttgcc agagatctgg ttaatttaag
     9361 taaaaagttc aagattgaat atttacagtc cgtcgacatg tttcctcaga ctgcacgttg
     9421 tgaagttgtc gtgaagatga cgagactcgc tgactaaatg aaagaggttg tgaaaaagtc
     9481 tgccctgtat caagtaataa gaacagccaa acaaaaatag ctcttcatgt tttatgaata
     9541 ctgtacttgt gaccgaagag ttaacaggag gacactattt atttaaaaaa agggttgtga
     9601 catagttttt gtcacagccc tttttgatta ttattggata ttcacgtcta tcacttgata
     9661 aaatgcattt gtcgtatcgc tgatattcca tactgctaaa atcacattat atccttttcg
     9721 atcctgcgga agtgtgatcg tttggttgac tgtttttggt ggaacagttg ctttgtcgtc
     9781 tatttttgtg atcaattcaa aatcactaaa tttcaaaggt tgattaggat tccaagttgg
     9841 tttggtaatg tagtaatccc aagtacttgt ccgatgttgt gctgttaaat tccaagtaat
     9901 atttaatgtt cctggattaa ccatgttttt atgccatctg ttagatgtct gttcatctaa
     9961 aggctcaaag ccgctgactc ctgcactagc aatttttcca tcaatgaatg tatttttggt
    10021 agcttcgata ctttgcggtt cccattgtgc tcttccgaca ttttgattta agtttcctgc
    10081 tgctgatgtt cctaggtatg cacggcttcc tggagaagta atataaccat gcgcaaaacc
    10141 tggaatgcta aaaccgaaca accctaaact aactaaccca ataaacaata acgtactaat
    10201 ctttttcatg tagaaacctc cttcatttga taaccacatt gtaaacgctt tattgtatta
    10261 acacaaacat tatgctttta taattttatg ttgaagtaaa aaaggtgaaa aaagtaagat
    10321 gcagattata acagaagtag acgagaaaag tacaaataga aaacatgaga atcaaatcat
    10381 atgatctttt tcaaaaacat cattcatata aacaaatgtt caacttttta ataacaacaa
    10441 ttatcaattt ttttcttact tcacaaacta aatacaggtc ttgctgtgag ccttcatggt
    10501 aaactaatat tatagaaagg agttagtaaa atgaagatga taaaagatat tcgtttttgg
    10561 gtttgtgtta taggaattgt catattaggc tttttatcag ggttgcttag cggaaatccc
    10621 ggaaaatatt actattcttt gcaacttcca ccatttgcac cacctagctg gatatttggg
    10681 ccgatgtgga ccttactgta tatattgatg ggtatttctc tttatttgtt acttaatcac
    10741 aacaacaaaa aacagcgaaa caatcttgtc ggtctttttg tgattcagtt catttttaat
    10801 tttatatggt ctgctctatt ttttaattta agaaatattt tcattgcagc aatcgatatt
    10861 acacttttag ttattttctt gtcagtgctt atgtatcagt tgtggctgca tcatcgcctg
    10921 gcaatgtggc tcatgattcc ttattattta tgggtactat tcgcaactct attgaattat
    10981 tcaatttatt ttttaaacta agcgctaaaa agagacgtcc tgtaatttaa cacatagaaa
    11041 aagtgccacc taagttagat tttttgtcta atttaggtgg cactctttat taaccaaact
    11101 attttttcta gtacccactt gttgttccat actcattatt ctggtcataa ccataaccat
    11161 tttgttcctg tccagcgtaa ggatcataac tttgagtatc ttgacttggt gtatagtact
    11221 gacttgtatc ttctgtttgc tgataatagg tattcggatc ttggtactga tcatatgtgt
    11281 tttctgaata ggaagaataa tcggtgtacc cttcactatt cgatccatca ccgtttgtcg
    11341 aatcgccatc ttccgaatca tcaaagaaca tcattgaagc attctcattt ttcaaattag
    11401 gtaatgtatc gcttgttttt atatttaatt gtttcttcag ttcattttgg atcgaaagaa
    11461 gatcattttt tcctaaaatt tgatagtaga tatcattgat catttgtcct tcgccttgca
    11521 actggtcttg tttgattgtt tcaaacgccg gggtgtagtt cgtcgctaca tcaagcatgt
    11581 catcccaatc taagtccgtc gtcacattgt tctcaactgc ttttaaaatt tttctataat
    11641 tgcttacgcc atctaaactt aaaactttat tgacgatttt cgtcactact tcccgctgac
    11701 gacgttgtcg accgatatcg ccttctggat cttcgtgtcg cattcgagaa taggctaaac
    11761 ctttttctcc attcaatgtt tgttcaccta ctggtacatg gacaccatcc aaagtaaagt
    11821 caattttatt attcactttg atgccaccga ccgcattgat caaatcttcc atgccatcca
    11881 aattgatcgt tacataatga tcaataggaa tatcgagcag ttgctcaatt gaatccatgg
    11941 ccatttcgac accaccaaat gcgtaggcat gatttaattt atctaccgtt ccataaccta
    12001 cgatattcgt ataaatatct cggtccaaac tgatgatcgt tgattttttt tgtttcggat
    12061 taactgttac taccatcatc gtatcagaac gtccttgttc cgttcttcct aatccaccgg
    12121 tatctaaacc taatagcaaa accgaaaaag gttctttgtc atcaatgctt actctctcag
    12181 aacgtttgga ggatattcga ttagagtttt tagaaatttt gtttactgtt tggctcgctt
    12241 ctcccataaa tctgattccg tatgctgata atccggcaat cagcaaaacg aataccccta
    12301 acaaggttaa tagaactttt tgccatcgct tcataataca cctctgtttc acattttcta
    12361 aaatattgtc tcatataact gttcaatcag caaattcact ttttaatcat aagaaaactt
    12421 taaaaaacat ttttgtgtaa cgattattat tttttaaaat ttctcaaaat tcttaactat
    12481 cataccatac cgtgaaaaag atcgaaagac cgaatacacg cagttcgcaa tcgttttatc
    12541 tttttttatg tattttttcc agaaaaagcg aacaacaagg tattttccaa ataatcgtgc
    12601 agtaaaatga tcgcacatac ctttcccatc tttttaacag tctttgccta ccgttcagat
    12661 tatcattcga tttcacgaca atttcttcct atgactggtc cttttgttaa acccatcttc
    12721 tattcgtttt atttccagct cactgcatgg ctcttaaaat gactcgaact agtgattgca
    12781 ttaccatcat cttttaatgg atagatatgc tcctcttcaa agtaaaacga ttttctttta
    12841 aagtaataga attgacatgg taatgagtat gtatactagt ggtttcacaa atggtctttt
    12901 attcaacatc caaaaactga aatttcttta aaatccatta ctaacgaaga aaatgttcct
    12961 tgcttgataa ttagttagga catgtcaagt gaacaaatgc atttacttag ccaaaaaatc
    13021 taaaaacttc ataaatagac aaaaagacac gactctctaa gaagtcgtgt ctttattcaa
    13081 caaattatta taatttagtc acttcagcag cttgaggtcc gcgtgcacct tcaacaatcg
    13141 tgaattctac ttcttgacct tcttctaggc tcttgaagcc gtctccttga attgctgaga
    13201 aatgaacaaa tacgtcatct ccgccgtcta ctgtaataaa cccaaatcct ttttcattgt
    13261 tgaaccattt aaccgttccg tgttccataa atgtatatcc tccaaaaaag ttcttgcaag
    13321 taaccttgca agaacattat acccaattta gaaaacgcta gcaagctttc gctcttttat
    13381 tttccaaatt atttttaaga ctgatatgac ggaggaaatg gttattttgc tgcttcgaaa
    13441 cgtttgttta cttcatccca attaataaca ttccagaatg ctgcaatata atctggtcgg
    13501 acatttttat atttcaaata ataagcatgt tcccaaacgt ccaaacctag aattggtgtc
    13561 tttccttcca tcaatggaga atcttgattt gcagtagagg taatagctaa ttttccattt
    13621 tccattacaa gccaagccca tccagaacca aatcgtccag cggctgcttt tttgaattct
    13681 tctttaaaag aagaaaaatc accaaaagct tcattaatcg cttcttttat ttctcctgta
    13741 ggttcgccac cagcatttgg tgccatgatt tcccagaaaa atgaatggtt agcatgtccg
    13801 ccaccattgt tacgtacagc tgtcttgata tctgttggaa tagcgtccat atcagataat
    13861 aattcttcta ttgttttttc gcctaattct gggtatttct caattgctga atttaaattc
    13921 gtcacataag tattgtgatg cttatcatga tgcagatgca tcgtttcttc gtcaatataa
    13981 ggttccaatg cgtcatatgc ataaggtaga tctggtaatg tgtaagtcat aaaaaaattc
    14041 ctcctcaatg ttttcttaat aatgtctaca ttacaaagag taacaaagag aaggaaattc
    14101 ttcaattgtt atgcctagtc gtctttttca ctttgaccta cttcttcttc aaaaacctca
    14161 gtttctttaa aaataaacaa cttgctgaaa atatagttcg caaggataac aataatttga
    14221 gtgatgattt tcgccacata ctcattgctg ttcataacat tgatcaacaa taccattgcc
    14281 cccatgtcca atccgtaaga aaggatacgg tagaagaaga acttaccgca ttcaataagt
    14341 gcatgtgtaa aattcggtga ttttgaatgg aatacccaaa gtttgttcgt tacaaaagca
    14401 aacaatacag aacaaaacca cgaaatagaa ttggataagg ccatacttaa atcaaaatat
    14461 tggcgtgcga gagcaaaact aacaaaattt acaatcgtag ccaaaccacc aaaaaagaga
    14521 tagactagta tctcccaata tccttttgct tctaaatatc cttttaactg tcgatactgc
    14581 ttcatgagcc aacctacctt attaaaaatt cttatttatc atactctata tttcaataac
    14641 tttcatcttt atttaaaaaa attaattttt tttgatcctt tgcctttgtc tctgcttgga
    14701 tcgtcacatg tgtaatacca tattttaatt ttaataaaga aattatacga ggatatatct
    14761 tttcaatctc gcttagtagc tgatcttcaa aatcaatatg tccgtcgaaa ataattatct
    14821 tttcgctata ctgccaaata tggacatgat gaacatcctt taccccatcg attgcttcaa
    14881 tttcttgttc cattttttca tagtcgatag caggtgcact ttgtatcaaa atagcggcag
    14941 cttttttgat caccattatc gcttctatca atatatatat cgaaatgatc agtgtaataa
    15001 ctggatcgat ccagtagata tcgaaaaaac ggatcaaaat tgctccgata atcacactta
    15061 ccgaagacag tgtatcactt aacaaatgca aataactaga ttttatattc aaactttcat
    15121 ctgcttcttg tcgtaataat aatactgaga acaaattcgc gagcaatcca ataactgcta
    15181 ctgttagcat caaatgacta tttatctttt ctggtgaagt gaaccgcttg aatgcttcaa
    15241 cggttaaaaa caaggataat atgatcaata tgaatgagtt gatgaaagca ctcaatattt
    15301 ctaaacgctg gtagccatac gtacgttttt tatttgcttc ttttcgggcc attttttgtg
    15361 ccccgtaaga aaaaataatt gccaatgtat cactaaggtt atggaaagca tctgaaacaa
    15421 gagacaaact ccctgacata atccctccaa taaattctga taaagtgatc accgcattca
    15481 aaaccactac tccaaatagt ttttttgatg atctctgtgg ctgtgtaggc actattcctt
    15541 cctccatttt atttatattg gatagtcttt agacaagaat aacatttttt tgatatttca
    15601 ccaatcattc agctgtttcc ttaaagcact tgtatttcac ttgatacttc gctagaattg
    15661 caagtgatga cactactaga tgaggagtac ttatgacaac aaaacaatta attatcagtt
    15721 cctttatacg attcaaagac ctaaaggagg cgagaaatgt cccattttgg aaagccattg
    15781 tctatctttt agttttgagt ataattatgg ctttgccaat ctcttttcaa gtttttcaag
    15841 tcttaaacaa cattcaattg gatgggcaaa agattgctac tcgcattcct gattttacaa
    15901 tccaaaacgg gcagcttcaa accgaagaaa aagaaggatt catttaccaa acaaactcca
    15961 ttatcttcac atttgatcca gagggaaaac gaacagaaca ggacatctct actgatttga
    16021 tggggaattt tgttagtgtc gggatgttaa aaaacaaact tatcatagct cttcctaata
    16081 caggcacgac ttctgctttg ttgaacaata accaattaga acttccctac accaatgaat
    16141 ctttaaaaaa cttaacaggt aagcagctcc gttctttttt aagtgaagca tctataccac
    16201 tttggataaa agtcctgaca tttatatttt ctatttatcc tagttttctg aatctgctga
    16261 tcactttgct atttaccaac attgctgctt ttctctatgc acgctttcga ctaacaaaag
    16321 ctacttttct tgattgttta aaaacattga tctattcaat ttcattgcct actataattg
    16381 caacaatctt gatggtcttt cttccttctt ttgacactag cgccttcatt gcttttgcag
    16441 gaatctttat ttttgctcaa gctgtcaaag gatgggcaaa aatatcgatc aattaagaaa
    16501 taaagacaac tcgttatttc tacttcaata gaccctttta gaggttgtga catttttttg
    16561 tcacagcccc ttttttattt gaaaaatatt tctttctcaa aaaacaagca aggcgcttca
    16621 aagttcttct tgtatgcgct acacttagag taaagaaagg cgggaaatta actatgtcag
    16681 aaagaatcat ttatgcagat cgtccatttc gtgatgaatt tatcgaagaa atccatacta
    16741 tagcgcctgg ttatatcttt aaaacagaat tacaaccaga ggatctacaa aatgtagaga
    16801 tcagtttggg ctggaactca gactaccaag aaaaactatt agcctcttct accttgaaat
    16861 gggtccaatc tatctcagct ggtgttgatc agctacccct tgagactttt tcaaagaaac
    16921 atattttact ttcaaatggt agtggcatcc acagtgaatc gattacggag catgtcttag
    16981 gaatcatcct tgggtattct cgtggtcttt ttcaagcaca aaaggcacag agtgacatga
    17041 aatggttagg ttcatcggtc cattaccaat ctatccaagg gaaaaaaata ctaatcattg
    17101 gaactggcca tattggaaaa atgattgcaa aaaaatgcaa agcattagac atgaactgct
    17161 atggaatcaa tacatccggt catccagcag aaggaatgga aaagactttt tctttaaatc
    17221 aactaaagga agttcttcct gaagtagatt tcgtagtcaa tatcttacct ctaacggagg
    17281 aaacaactgg gctctttgat cagcatatgt ttgaacactt tgatccaaaa tctgtattca
    17341 tcaatgttgg ccgtggtgct tctgttaaga cacaagatct ggtgcaggct ttaaataacc
    17401 agcaactttc ctttgcagca ttagacgttt ttgaggaaga gcctcttcca caagatcatc
    17461 ctctttggaa aatggacaat gtactgatta cttcccacat tgcaggtttg acacctgatt
    17521 ttcaaaagaa attaatggct atttttttaa ccaatctgaa aagctacttt gccacccatg
    17581 agcttcaaac caatcaagta aaattgtctg caggatatta atagctgaaa acaata
//
LOCUS       DAZKKW010000056        17388 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.057, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000056 DAZKKW010000000
VERSION     DAZKKW010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17388
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.057"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(88..633)
                     /locus_tag="ACG8PO_001816"
     CDS             complement(88..633)
                     /locus_tag="ACG8PO_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HFQ0207124.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDSVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(645..1337)
                     /locus_tag="ACG8PO_001817"
     CDS             complement(645..1337)
                     /locus_tag="ACG8PO_001817"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFQ0207125.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(1356..1640)
                     /gene="macP"
                     /locus_tag="ACG8PO_001818"
     CDS             complement(1356..1640)
                     /gene="macP"
                     /locus_tag="ACG8PO_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall synthase accessory phosphoprotein
                     MacP"
                     /protein_id="HFQ0207126.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(1653..2216)
                     /locus_tag="ACG8PO_001819"
     CDS             complement(1653..2216)
                     /locus_tag="ACG8PO_001819"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFQ0207127.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            2444..3106
                     /locus_tag="ACG8PO_001820"
     CDS             2444..3106
                     /locus_tag="ACG8PO_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HFQ0207128.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            3230..4426
                     /locus_tag="ACG8PO_001821"
     CDS             3230..4426
                     /locus_tag="ACG8PO_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HFQ0207129.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(4527..5903)
                     /locus_tag="ACG8PO_001822"
     CDS             complement(4527..5903)
                     /locus_tag="ACG8PO_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFQ0207130.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVIILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(5940..6806)
                     /locus_tag="ACG8PO_001823"
     CDS             complement(5940..6806)
                     /locus_tag="ACG8PO_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HFQ0207131.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMNYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(6895..7920)
                     /locus_tag="ACG8PO_001824"
     CDS             complement(6895..7920)
                     /locus_tag="ACG8PO_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFQ0207132.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(7938..8612)
                     /locus_tag="ACG8PO_001825"
     CDS             complement(7938..8612)
                     /locus_tag="ACG8PO_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFQ0207133.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(8796..9689)
                     /locus_tag="ACG8PO_001826"
     CDS             complement(8796..9689)
                     /locus_tag="ACG8PO_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFQ0207134.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(9763..11460)
                     /locus_tag="ACG8PO_001827"
     CDS             complement(9763..11460)
                     /locus_tag="ACG8PO_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFQ0207135.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(11453..12766)
                     /locus_tag="ACG8PO_001828"
     CDS             complement(11453..12766)
                     /locus_tag="ACG8PO_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
                     /protein_id="HFQ0207136.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPHAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESTEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMAELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(12766..16707)
                     /locus_tag="ACG8PO_001829"
     CDS             complement(12766..16707)
                     /locus_tag="ACG8PO_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFQ0207137.1"
                     /translation="MEKLDEPIVPSGQALQRIQRQLFNGILERLGPTATQDPYEYIYP
                     SGEVENEQHRYPKFTTNDYTKVLGNWTQSNERYQGDKGSPYDGENLIDDSDYTNGGAN
                     WRNYDYATNNGSTAGEEDRNVPAQTVQLWGNERNFENSYLDYNGAYIKKWVEPVLPSS
                     TASDLHPEDATTLYNVYLDVIGGEKKEISPIDIVFVLDKSASMSELTAGTNSQTKNAA
                     LIEAVNEMSKDLLSDPSLDIRIGMVNFYHNSTAINNHEQISSDIFPLTNDINRLTGSE
                     NTALNRTPIGGTPLTLGLKNGYETLYKDNGGENRNPEKILIVVGDGTPTFSYAPIQSS
                     YRTSTNGAWSNWTVMEDKIAEDNDVLFRNFEEFSGNTSNAGFTHPVTYASDFNRPEDE
                     VNVHYRYGEVKEGDNKATHWVGDGSSNNNTNGSPTSQEKSSAINTVAYHHWLKNKYQE
                     NPPSIFSIGLGIDGNVSGRQRLDAIGRNVLKNIADLEEDGVTPRYYNANNKNDIVTAL
                     EDISSTFKRTIQQATLYDETGFNVSPFGAGRSADIQFYHLDPGENGMKYQEPEIWDEE
                     RHGQAPPEVVATPTTYVGRENQAYKFSPISLGEGEMVRIKYQVKLDAGAQDGNFCTVN
                     NMAYIQNLEDYENDITTGRMYFPSSSIRYEHKGRNFQVSKKGQNGEPLAGVEFALYDQ
                     NPESGEAEPIETSKTTTDGIAIFETKMPILGDETGAVSDIFWIREIAGPPRYQLVDET
                     YQFQIKKYAANEDEMLGHYELVDEYTSGSRVGGPEGKDPNSYYEGEYHKLSVVQEPNI
                     EGTEYDELYVKLEMRNEFKPIHMNINKLIEGSNMPINGAEFELYETDGNGLEIGYPVA
                     KGLSGVNEVEEEKGRLTFYSVDEDGKYVIVDGEKVIHPIGEPDSFVYKDSEETTNYAE
                     YKIIESQNPEGFKEPDENDTWILRLYDNAIGTIQLKQTGEENWQYLTYTTDEEDRLWV
                     AFTAYNTFDYREIRVRKLDHEGHTLDGAGFDIRKVGDSSFPLYRAYTGNPLEPEESKR
                     LPGIGYFYSRAVGVEKYVDLNYDQPLHLSIGEYEVSESVAPNGYRLSDQVFQFALNEE
                     GKFIIEDTVIEDETAPLPEGYEIIDGVLQVTLEDELAPIDLELLKIDSMNSRRLEGAE
                     FSIEKQNSAGEYEIIEGGLAPDPNDSSLFNASDLLEGVYRIKEVKSPDSYRKLPGYFI
                     LEISYREEPDIENDRVVPGKEAGTLKVEISYYPNEEAETPDLQQELEYELLETNRIRL
                     QLSVGNDPENPLPATGGSGRLFYILLAAFFMALAGAAYGLFVRQSKKEGAR"
     gene            complement(16816..17274)
                     /locus_tag="ACG8PO_001830"
     CDS             complement(16816..17274)
                     /locus_tag="ACG8PO_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFQ0207138.1"
                     /translation="MKGLKLKLLQLVTVSLLFGPYFQSVYHMTTLDVAAESTETKEDE
                     VFLDEDYAKIEGKYIEKEETLEWHLAFEKKESANEGRIRLAIDAAAAGIGTVKNVRGT
                     GLSSYDEDGEDLKVETIDGQEWYVGKVYSKEAETGTLIFETEKIPDVNEG"
BASE COUNT         4876 a         3900 c         3019 g         5593 t
ORIGIN      
        1 taaaaaagag cataaaacaa agtcgtttgt tttatgctct tcttttatta ttttgtgctt
       61 tttattttat caaatcactt tgttttttta taaaatttct gcgcctagtg tggactcaaa
      121 atgtctcaat gcccattcat gtccaacggg atcgaaactt gccacggaat ctttgaaaat
      181 atggagtttg tatcctagat tatacgcatc gactgctgta tgcaacacac agatatctgt
      241 acatacgccg gtcaaataaa tatctgtgat ctggcgttct cgcaaacgga tgtctagatc
      301 tgttccacta aaagcggaat aatgccgttt gtctatccaa taaacatttt cctcttgctc
      361 atagtcttgg tataaacttt ttaaagagcc gtacaattcc cgtccacccg ttcctatcac
      421 attatgcgga ggaaataagc ggttctccgg atgaaaggaa tcttttggat catgcgcatc
      481 aatggcaaaa acgacaaaat ccttttgatc aataaacttc tttgtatgag cagtcagctt
      541 tttttcaatt acttgtcctg ctgtcccagt agtcagcttt ccatcctctg caacaaaatc
      601 atatgtgtag tcaatcgaga tcaatgcgtt catcttagtc ctccttatac gagatgtttg
      661 acaaattcga tcaccatttc tgctgatcgt ttgcctgcgt cttcgataaa ttcgtcaaag
      721 ctttgtgtcg ccgagtgatc tgctgtgtcg ctcattgcac gtacgatcaa aaatgggata
      781 ttgaactgac gagctgtttg tccaacagcg gctccttcca tttcgcaagc taatgcttca
      841 gggaaattcg ttaagatttc tttgatttta tctggcgagt cgacaaatgt atcacctgta
      901 acgatcaatc cttcttttgc gttcaaattc gttgcttttg ctgctttaac catttcactg
      961 cgcaaatatg tgcttgcttc ataatataga ggcattcctg gtaattgtcc tgggttgtag
     1021 ccaaatcctg ttgcatctgc atcaaaataa gctactttgt ctgatataac gatgtcacct
     1081 acttgtaagc cttcaccgat tccgccagct gaacctgtat tgatcaccat gttcacgccg
     1141 tattgttgga tcaacagact tgtggtaata gatgctaaga cttttcctat accggaacgt
     1201 acgacgatca cttcgtggtt acctaatgaa cctgaaataa ataaagcgcc tgcgcgttcc
     1261 catgataatg gttcgcttag gttctctctc aaaattttta cttcttcttc cattgcgccg
     1321 ataataccga ttttcatctt tttaattcct tctttctata taaatgcgat tgctaaaaaa
     1381 accacacata acattaggat aacaataata agagatttca tcaaaaaaga gttccatttg
     1441 gtcgtctttt cccgttcgct gatccttgtt ttcgtgattg gtttattctt tttgctttct
     1501 ttacgataaa agctggcaat ctttttttct tcttgctgat aagctgtttc tgcttttcgc
     1561 tgttttttta atgactcgct tgcttgtgct tgttgtcgct tgcgaagttc gcttcgcgta
     1621 ataagcggtc ctttggccat aagccgatcc ctctactttc ctttggtcaa tagttcccag
     1681 tattgaatcg cataaatcgt ctttgcatca caaatcaact gatctttcat cgcttgttgc
     1741 gcctcttcaa gagttagatg atacaactcc agtacttcgt cttcatcttg tgctaaagga
     1801 ttctcgacct tctctacccc ttgtgcatga taaatatgaa ggacttcatt tgcaaaacct
     1861 ggtgaaagat acatcgaagt aagatggctc agcgacttcg cacgatagcc agtttcttct
     1921 tctaattctc tagctgctgt catttcggga ttttgtcctt ctcctggatc gatttttcca
     1981 gctggaatct ccaaaatcac tttttctaaa ggttttcgaa attgtttgac aagaagcaga
     2041 cgatcttgtt cgtcaaaagc aatgatcccg acaccgccag gatgaaatac cagttctctc
     2101 ttcgaagttc ctccatctgg caaacgaact tcgtctacag caacatcaat aatttttccg
     2161 ctatagattt ctttgcgact gattgttttt tcttcaaatt gctcattgtt cagcatatct
     2221 tctcctgctt tctttgtaat cataactgct tttttttcat ttttcaattt ttctcaaaaa
     2281 ctccttatta gtttaccata gataaacctt agattttctc taaatataga aaatccttct
     2341 ttagtccgat actctttcta gtagtccgta tgctaaaata agtatgtaat cgaataacga
     2401 aaagaattca atactcaacg ataaatgaca tggagggttt attatgataa aacgaaattg
     2461 gccgtggctt ctagcattgg tcttgctatt tctctttgtc cgaaacaata gtttttcttt
     2521 tgttctagga cttttattga taggtgtagt tttagttttg tggggactct ttggttcgcg
     2581 caggaaaaag ggaaaacaag aagaaattcc tgcactgact gacgaacttg aaactcacta
     2641 tgaaaaaagc ggtatgacag cgaatgaaat ttcatttttt cgacaaacaa tgaatcaaac
     2701 aaaaaatgag atcaatcaat tgcaaatgaa tatgcagcag acagccaaac ttaaagcaat
     2761 cgatctccgc catgatccag tcaaagcagc gaaagcttta ttcaaagaat tggtgaaaga
     2821 accaaaccga ctgcatgaag caagtcagtt tctttatacg catctgccga atctagttga
     2881 tttgacaaat aagtataatg agatcaatga ccatgagatc aaaaacaaac agacctatga
     2941 aaaactagaa gaaagcgcgc agatcatcga ccagctgtca gctttaatcg cacaagatta
     3001 tcaaaaattc gttgctgagg atctagatga tatcgacgta gagatatctg ttgcgaaaca
     3061 gagcttaaag cgtgataata aatatgaacc agaacaaaag gattaactct tttgttcata
     3121 aaagaagact cggaacattc agatgatgtc tggctgtttc gagtactttt tctaaagtag
     3181 agacattcat gtaagaatcg agaacagatt caacaaggag gaatttttta tggatcataa
     3241 accgactgaa acaaaagatg ttacaccagt taacgataca cttgaagatt tattgaacaa
     3301 cccgttttca actccaacag atacgctgac cacttcacaa caagcggaga ttgatcagct
     3361 ccaagagcaa cagacagctg ctcgtttaat tgacaaacta ccggcggaac gccaagaaca
     3421 agctaaacag ctagcagcaa aaatcgatgc taatgatgca cagtctgtca tcagctacgg
     3481 atcggctgcc caagcaaaat taagtgaatt ctcacaatcg atgttgaatc atgtgcaagc
     3541 acaagatatt ggtcctgtag gagattcctt aactgaactg atgtatcgtc tccaagaagc
     3601 caatcctgac gaactgcgtg ccggagaagg gaacatcttc cagcgtgtct tcgggaaggt
     3661 caaacaatcg atctatgaag tgacagcaaa atatcaaaag attggtgcac aaattgacaa
     3721 gatttcagtg aaattagata aagaaaaaga cggtctgcta aaagataatc tgatgttaga
     3781 acagctttac cagaaaaaca aagactactt tgatgcactg aatatttata tcgctgctgg
     3841 agaattgaaa atggaagaac ttcaaacaac gatcattcca gaagcgatga aaagagcaga
     3901 agaaactggc gatcagatgg atgttcagat tgccaatgat tacacgcaat tcctagatcg
     3961 tctggataaa cgtacgcatg atcttcgctt agctagacag atcacgatcc agcaagcacc
     4021 acaaatccgt ttgatccaaa acacaaatca ggccttggct gaaaagatcc aagcatctat
     4081 cgctacagcc attccgctgt ggaaaaacca agtcgtgatc gcattgacct tgcttcgtca
     4141 aaaagatgca gtaactgctc aacgccaagt ctctgaaaca acaaacgact tattgaagaa
     4201 aaattcagaa atgctgaaaa tctcagccat cgaaacagcg aaagaaaatg aacgtggaat
     4261 cgtggatatc gagacccttc aaaccactca aaatgatttg atcgaaacaa tccaagagac
     4321 attgcgtatc caaaaagaag gaaaagaaaa acgtcgtcat gcagaagtag agcttggaca
     4381 catggaagaa gatctgaaac aaaaactgtt ggatttaacc caatagatca cctttcgctt
     4441 gttactaaaa caaacgccca acaacagttt attacaactg ttgatgggcg tttttcttat
     4501 ctttcttcta acttctccat tggagtctat ttctcattca tcttgctgtg tccatagcca
     4561 tagccaaaat aaatgatcag tccaataacc attacgatac cgaacacgat ccatgtcaca
     4621 gtttgcagac gaagcatcaa tacaatacaa gtcaagatgg agacgattgg caatagcgga
     4681 acgaatggca ctttgaattc tccagctttt ggtttgccaa acatttttct caacttcagt
     4741 aagcctagac taacaagcat caaataggcg agcgccatga tattcgttag ttccgctaat
     4801 acattcaatg ggaaaaatcc ggcaaagaat acagctgcca caccagcggc ataagtcgct
     4861 tttttcggtg tacgatgctt ttgatcgatt tctgtcataa acttaggaag taagccatcc
     4921 ttgctgacag caaacagtaa acgagcaagt gaatacatca tcgagatcgt taccgtcagt
     4981 agcgttaaaa ttgcccctac agcgatcacc gcgcccactg ttccatgtcc tacatacctc
     5041 attgcaaaag ccactgggtc attgactcct agttcagtat acggaacgat tcctgtcaag
     5101 atcagcgtca cgatgatgta aaggacagta gcaatcagta tcgaaccaat gattcctctt
     5161 ggcacatctt tttgcgggtt tttgacttcc tcggctgcca ttgcaacggc atcaaatccc
     5221 aagaaagcaa aaaacacaag agctgcacca tctagcacac cggaaaaacc aaaaggcatg
     5281 aatggctgcc agttatcagg tttaacgaaa aatattccta caatgataaa caacgcgatg
     5341 ataccaaatt tgacatatac catggcatta ttcaaacgta atgctttctt ggcttcaagc
     5401 gacacgatgt aagtgacgaa tatcactacg agcaccgcaa tcaagtcaat gtatgtacca
     5461 ttttccggat tgtacccgcc agttaatgct tgtggaagtt cggcccctaa acttttcaaa
     5521 aaaccttgca tatagccaga ccatccggaa gcaacagagg aaaccgctag taaaaattcc
     5581 ccgatcaaca accaccctgt cagccaggca gcaaattcac caaatacaac atacagatag
     5641 gcatatggcc caccaatcac tggcactcgc gaagcaaatt ctgcgaaact tagtccagac
     5701 aaaatgataa cgattgcagc taagacaaat gaaagtgaca gtgctggacc agcattttga
     5761 ttggccgcaa cccctgtcac cacaaatatt cccgtaccga tgatcgcacc aattccaagc
     5821 atgatcaaat cagacgtttt tagttctttt ttcatctcac tcgtatgatt gagattgatc
     5881 tctttcttac gaaacaaatc cattgaagtt cctccccatt ttaggttgga aaacagcgat
     5941 taaactgttt tcatataccc taaaaggata ccacctaaaa tgactaaaag gcaaccaaca
     6001 ataacataaa tgagctcttt tttcgttttt ctttcgccaa ggatgaaaat cccacctaaa
     6061 gtagagataa tgatccccat ttgtgaaagt gagaagccaa cagctaaacc aagtgatttg
     6121 actgttagaa gcatgcagat gtttcctaag ccccataata atccacagat catgtttttc
     6181 cacacaaact gatcgacttt aactttacgg aaagcaaaga agcttgcgcc taagatcatt
     6241 ccgatacttt gaggtaagat aattgacatc gcatccaaat tcgcccagtt gacgatgatg
     6301 gtatatacac cataaccaat cgtagagaag atcaatgctc taaaaccctt accaaaatca
     6361 agcattttat tatctgtatc tatttttcct tcgtcatctt gtcgggcagt caaatacgca
     6421 ccgcttacaa gaagagctaa agcaataata ccgtatacat aatctttcgt ttgtgtccat
     6481 tcatggaaga aaacggctcc cgcaatcgta ttcaaaatta attgaaaacc agtagacaat
     6541 ggtagcccta cagaaacacc catgtaattc attccatgga actgtccatt ttgtccaaca
     6601 ctccaagcta agccggataa aaatccgatc aagaaaatcc aaggggtgat tacaggacta
     6661 acaacgaaaa agacaactaa agaaaacaga aatgcaccga tcgtcattcc tagtgtttgt
     6721 tgattggcat cgccaccaat tttaccactt actaaaccaa tactacccca tgccaacata
     6781 gggactaatg ctattaaaat ttccataacc aatctccttt tatttcacat gataagaaac
     6841 ttatcagcga aaacgttaac aacacaagca ctttttttcg tttctaacga ttttctaacg
     6901 tttttcatta taaaaattta agattgggaa aaataaagtc attgttgtcc cttttccttc
     6961 agttgattca gccgtcaaat ggatacctaa tttttcggcc agattttttg ctaaataaag
     7021 tcctaatcct gttgaatgtg ttttgctaag tcgaccattt ctcccagtaa atcccttatc
     7081 aaagatccgt ttcaagtctt ccgctgggat accgatccct gaatctttaa ctgaaaggct
     7141 gactccttgc gcttcctttt ctatgctcac aatgatgttt ccgtgatccg gcgtatattt
     7201 gatggcatta ctaagcaatt ggttgaagat aaaagcgatc catttagcat cggtcaaaac
     7261 agtatgatcc tcaccaacga tctcgtagcg aatatttttt tggataaaat aatttgcctg
     7321 actgcgtatc acaggctgaa tgattccttt tagtccatat tcttggatga ggtagtcccg
     7381 cgagaaactg tcgagtcttg cataatacaa aacttgttct acgtattcat cgatttttga
     7441 taattcattt tctagtaatg tatacttttg atcgtcaata tcgaactcga tcgaacgaac
     7501 tagcaattta gaagcggaaa gtggcacttt gatttcatgg acccatgagt caatataatc
     7561 tttttggtct tgttgattac tgattgctga ttgcattact tcttggtgtt cacggatcag
     7621 ctgattgata tattcttcta ccagtttttc ttcttctgtc ctcccaccac tcaaataatt
     7681 ttgcaaaggg gaagtttttt gtatgtcggc taattttccc caccatcttc tcttgctcaa
     7741 gtaaccaccc agcagataaa aaatcagaaa gacgccctca atcaaagcta aataagcaat
     7801 tgtgctcaaa tcaactttga tgtttggcgc cagccacatc acaaagatcg tcaatgcaat
     7861 aaaaaacagc catcccaaga tcaatgtcca ctgatctttc aaatatttaa aaaaattcat
     7921 cttaaccgct catcctttca gggaacgata tatccctgtc ctacttttgt ttcgatatat
     7981 ccttggatac ctgcctgttc gatttttcgg cgtaaccgat tgatgttgac tgtcaatgta
     8041 ttgtcatcta caaatcgttc gtcatcccac agtgctcgca gcaatttttc ccgacttaag
     8101 atctttccat tgtgcttcat caaaataaat aatagacgat attcattttt gctcaaatca
     8161 atgatctctc cgttgatttc catacttccg ttgtcaacat tcaacgtaat gccattgtgg
     8221 ctcatcattt cactagataa ttctgtgtag ttgtaagaac ggcgaagtaa tgcattgatt
     8281 tttgcaatca gtacatcgat ttggaaatgt tttgtcacaa aatcatctgc ccccatgttc
     8341 atagccatga tcatatccat attcgtattc ctgctggaga taaaaatgat cggtactttc
     8401 gatacttcac gtattttttg acaccaataa tagccatcca aaacgggaag attaatatct
     8461 agtaatacaa gctgtggttc ttctctttca aagtctgaga acacttgatt gaaatcggtc
     8521 gttccaaagg tttcaaattg ccatttctca agttcctcca tgatcaattg tcgaatgatc
     8581 tcttcgtctt cgactaccat gatttttgcc ataaaaaatc cctccatctt tcttcttgct
     8641 actacaatat caaagaagcc cttgtgaaac aagttggctt acctggaaaa tgtcatttta
     8701 gcaaacgtta aaaaaaactg gaaatatgcg atttaatcgc gtttgtctct tccaagcagc
     8761 ttaaatttat tatgttattc ttaataattg gtctattatc tgttattttt ctttcgttgt
     8821 ttttgtcttt taaattttcg ataacaccaa taaccgaaaa gcgttagcac tagcaatata
     8881 cctagaagga gtataagtaa tcgataaaga tggtattttt ttgtctgttc tatctttgaa
     8941 gagacttctt ctgtaaaggg aactcgtctt cctgtcacta gtaatcggtg agtgttcacg
     9001 gtgtaaggtg tacaagtgat aagtgtgact agatcctgct tttctctaat tgctaatgta
     9061 tttgtttttg ttggaaggac aacaattttc tcaacgactt cataagcgag catccgacca
     9121 tacacttcaa gatagaatcg gtcatcttgc tccatttctt caagatccgt aaagagtttt
     9181 ttctcagcaa taccgctatg ggcggtaatc acggaatgtg tactctctcc tccagttgga
     9241 aaagaggtgc cttgcaggac tgtcgctcct ctatctaaca aaagatcatt cgtttcatca
     9301 aataaaggca agctaacatt gattttcgga atatagatgg ctccgatcat atgttgttcg
     9361 taatattctt ttcctggatt ttggacattt ctaaccgctt gctcaaaagg atctttgact
     9421 tgtcccattc ctgggattac tgatgtatga gcaagcgtct gatttttctt ttcatgcact
     9481 gctaattgtt gctcatgttt ttcttcttta tcgcgttcaa gctgcttttg ataattctcg
     9541 atccttcttt gatccaaata attgctgaga gcatctgcta aaaatggata tgtaaaaact
     9601 gcagctccgg caagaaacat cactgccata aataattgta ataaaagact gatttgggtt
     9661 tgaggtctat tacctatttt ctttttcatt ttcttcacct tcttatttaa aaaaactgca
     9721 gcaaccttta taaaaccgat tgctgcagct tatttttgtt gcttatactt cagacttcat
     9781 tttagattta cggtaccagc tgtacgctcc aatcatcaaa cttattccga ttagtaagaa
     9841 tgccaagagc ccgttcccac cagtagcagg aagtgacccg cgtttcatat tcggtactct
     9901 ttctggccct ggattttcta ctcctacgat ctgaatttct tcattatagc ttccataaga
     9961 gatgataaat tcagtaaagg ctgcctcacc tgttggaagt acataacctt ctggcgccat
    10021 ggtctctttc atttgatagg ttccataatc taacccttga acctcaaagt atccatttcc
    10081 atctgcgaca aattctgttg cttgtccgct tgttgcccac tcaattctat ctgctcctga
    10141 ttcataaaca gctaaaggcg actccccttt ataaaatatt gcgtattcag aacggtcttc
    10201 attccacaat tcgaatcttg catctttaag caattcactg cttgatgcat catatttttc
    10261 aaactttctt ccacctgttg tgactttctc tggcggctct actccttctt cataatcaaa
    10321 ttcatcattt cgtccgattt caataaccgc tgtgttgtta tggaaatcat ccgcttctag
    10381 ttctgtggaa accttcaaat tatacgtgat cgttaaatct cctccacgtg catctgctaa
    10441 tctacctaaa gtatctactt tggctactcc attttcttct gtcaaaatcg tcacgacaaa
    10501 cccgttgctt actacttcta ctatataatc ttgtcctttt gttaaaatcg tctcgccggc
    10561 tcgcacttct atttcctgat cataataagt tagaccaact gtaggcgtat ctgtgatttt
    10621 gtaatttttg ataacagcgc ttccgttttc aaggacagaa tcgatgaaat aaggaatagg
    10681 tgctgtgatc gtgtaagaag ccgtttttcc tctttctaga tcaatataag aaatcgtttc
    10741 ttgttctcca gcttcattaa taatcgttac ttgtctaagg tcgctttcct ctacattcat
    10801 ctgtttatct gcttctctga tttcgttttt cggatacaaa tgcacatctg tattgtatgt
    10861 gtctttccat gtagaaccat cccacacgcc atccaccata tcaggcatca tcactggcat
    10921 cataacaacc attggagacg cgattctgga aatatgagct ggcgaataag tttctaagaa
    10981 aagatagact ttatctctag aagaaggtgc ttcttccgtc acttggacgg tagagaatac
    11041 cgcttctcca gcttcattcg ttgtttgttt gtcaatactt gtcagtcgat attttaaaaa
    11101 ccaactttct gtgtgccgtt tttgaatcca ttcaatcgct tcggcagaat taagaccgtc
    11161 ttctcgttcc ggtgttaaag gattatctcg aatcaaatcg taatatactt ccgttacgtc
    11221 aaacaaatca aaatcgactc ctggaatatt ttcttcacca aaatccgtag aaaccaatcc
    11281 gctattcggt cgttcttccg gaacagatga gaaacctttt ttgtgtaatg taatcgtcac
    11341 ttcacttggt ttttgtgcga cttcttcggc tttgactagt tttccgttcc cgaaaatccc
    11401 tattcccgca gtaacgagta acagtacact cattcccgtg acaatcattt ttttattcat
    11461 ttttttctcc ccgttctttt tttattaata aaacagcacc taacaataaa agaattccta
    11521 gtaaagaaat caacgttctt gcttcattcg tttgcgggaa tacggaccat tttcctgatt
    11581 gacctggatt gcttggacca ctcggctctt tcggtttgtc cgttgtttct tttacggtct
    11641 cgttataaac acgtggtgtt gcggattcat aggcagagac tggaactttc ccggattcta
    11701 attcagccat ctcctgtccg ttaactacta catattgtgg ttggcctttt gcatccaccc
    11761 aaaattcagg gatgatgacc tcgattctct tacttgcttc atccatctcg taaccttcca
    11821 caccttttaa ttcttcaaaa taataggtgc cagaaggcaa gaaacgttgt cctgtcgtaa
    11881 caaggccctc tttatccgaa atgaatttgc ttacattttt gtcattcaaa ggatcattgt
    11941 ctgctggttt gatccattga tttttcaagt cattagcagg agccatatct aaataatatt
    12001 tccccccttc tatcatctga tatagagcaa aaacagcacc ttctaatggt tttccttttt
    12061 cagttgttcc tctttgacgt ccatacttga agaagtatgg atctcttaga taaccgacat
    12121 ttttaggata gatatgaatc tcttgcagtt cctcggttga ttctgtcggg tgataaattg
    12181 gcaaaatagc agcaattggg atagctgtct gatcgatatc aacatccaat ccaacttctg
    12241 gatcaatcga ttcttcgaaa atcaaataaa ctgcatcccg atcttggttt ttcttaggtg
    12301 ctttgatacg tgcaatcccg tctcctaatc cgccaaccgt atctttccca gttgttaccg
    12361 aagtccctat ttggacaagc ctttcttctg tgattagctg acggatattg tttctgttcg
    12421 tttcagcgat ttgctggacg aattctttag gagtcatgtt ttcagcagct tttttttcat
    12481 gataataatc ggtcatgtcg taaatggcaa atgtcgctcc atttagtggc accgtctgtg
    12541 aaatgatatc tgctgtttcc cctgcttcct cagagatagc caagccatca ttttcatggt
    12601 attcaaattg atcgttataa tcgatatctc gaatcatccg tttatgcaac acgaattcca
    12661 cgacatcttc ccctgattct tctgccgaca tcgcatgtgg aagaaggact gctgcaagcg
    12721 gtagtaaaac aagtaattga agcatcaaac acaacagctt tttcattacc ttgctccctc
    12781 ctttttagat tgtctgacaa acaaaccata ggctgctcct gcaagcgcca tgaagaatgc
    12841 agcaagcagg atatagaata atcgtccgga tcctcctgta gcaggcaacg gattttcagg
    12901 atcgttcccg acactaagct gtaatctgat tcgatttgtc tccaaaagtt catattctaa
    12961 ctcttgctgt agatcaggcg tctctgcttc ttcgtttgga taatacgaaa tctcaacttt
    13021 tagtgtaccc gcttcttttc ctggaactac ccgatcattt tctatgtcag gttcttctcg
    13081 ataactaatt tctaaaataa agtaacctgg aagttttcta tagctgtcag gtgacttcac
    13141 ttccttgatc cgatagaccc cttccaacaa gtctgaagca ttgaataggg aagaatcgtt
    13201 tggatctggt gccaagcctc cctcgatgat ttcatactct cctgcactat tttgtttctc
    13261 aattgaaaat tctgcgcctt caagacgtcg gctattcata ctgtcaattt tcaaaagttc
    13321 caaatcgatt ggcgctagtt catcttctaa ggttacttgt agtacaccat cgatgatttc
    13381 atagccttcc ggcaagggag cagtttcgtc ttcgatgacc gtatcttcaa tgataaactt
    13441 accttcttca ttcagtgcaa actggaacac ttgatcagat agccgatacc catttggtgc
    13501 gacagattcc gagacctcat attcaccaat agacagatgc agaggttggt cgtaatttaa
    13561 atcgacatac ttttctactc ctacggcacg agaatagaag tatccgatcc caggcaaacg
    13621 tttcgattct tctggttcta acggattacc agtgtatgct cgatacagcg ggaaggaaga
    13681 atcccccact ttcctgatat cgaaacctgc accatctaat gtatggcctt catggtctaa
    13741 ttttctaact cggatctccc gataatcaaa cgtattgtaa gcagtgaatg ctacccataa
    13801 acgatcctct tcatctgttg tatacgtgag atattgccaa ttttcttcac ctgtttgctt
    13861 gagttggata gtccctatag cattatcgta aagtcgcaag atccatgtgt cattttcatc
    13921 aggttctttg aaaccttctg ggttctggct ctcaatgatt ttgtattctg catagttggt
    13981 agtttcttca ctatccttat aaacaaatga atctggctct ccgattggat gaataacttt
    14041 ctcaccatcc acgattacat atttcccgtc ttcatctact gaatagaacg tgagtctccc
    14101 tttctcttcc tctacttcgt tcacaccaga aagccctttc gctactggat atccgatttc
    14161 cagtccgtta ccatctgttt cataaagttc aaactctgcg ccattgattg gcatattact
    14221 gccttcaatt agtttattga tattcatgtg gattggcttg aattcatttc gcatttccag
    14281 tttgacatac aactcatcat actctgtgcc ttctatgtta ggctcttgta caacagaaag
    14341 cttgtgatat tctccttcat aataggaatt cggatcttta ccttccggtc cgccgactct
    14401 cgaaccagat gtatattcat ctacaagctc gtagtgacct aacatctcat cttcgttggc
    14461 tgcatatttt ttgatttgga attgatacgt ctcatctact aactgataac gtggtggtcc
    14521 agcgatctct ctgatccaaa atatgtcgct gactgctcca gtttcatctc ctaaaatcgg
    14581 catcttcgtt tcaaagatcg ctattccgtc cgtggtagtt ttacttgttt cgattggttc
    14641 ggcttcccca ctctctggat tttggtcata taatgcaaat tcgactccag ccaacggctc
    14701 gccattttga cccttcttgc tgacttggaa attacggcct ttatgttcgt aacgaatgga
    14761 agaagatggg aaatacatcc gtccagtagt gatatcattc tcataatcct ccaggttctg
    14821 aatataggcc atattgttca ctgtgcaaaa atttccgtct tgagctcccg catcaagttt
    14881 tacttggtac ttgatcctga ccatttcccc ttcacctaaa gaaatcggag aaaatttata
    14941 agcttggttt tcccgtccga catacgttgt cggagtcgcc actacttctg gtggggcttg
    15001 tccatgacgt tcttcatccc atatttctgg ctcttggtac ttcatgccgt tttctcctgg
    15061 atctagatgg taaaactgaa tatctgccga tctgccagct ccaaatggtg agacattaaa
    15121 accagtttca tcataaagtg tggcttgctg gatcgttctc ttgaaggtag aagagatatc
    15181 ctccaaggct gtaacaatgt cattcttgtt gttcgcattg taatatcgcg gtgttacgcc
    15241 atcttcttct aaatcagcaa tatttttcaa tacatttcgt ccaatagcat ctagtcgttg
    15301 tctcccgcta acgtttccat cgattcctaa accaatggag aagatacttg gcgggttctc
    15361 ttgatacttg tttttcagcc aatgatgata agcaactgtg ttgatcgcag atgacttttc
    15421 ttgcgaagta ggactgccat tcgtattgtt attagaagaa ccatcaccaa cccagtgagt
    15481 cgctttatta tctccttctt ttacctctcc ataacgatag tgtacattta cttcatcttc
    15541 tggacggtta aagtcagatg cataggtaac tggatgagta aaccctgcat tagaagtatt
    15601 cccagaaaac tcctcaaaat ttctgaacaa aacgtcatta tcttcagcaa tcttatcttc
    15661 catcacagtc cagttgctcc aagctccatt agtgctcgta cgataacttg actgaatcgg
    15721 tgcataactg aaggtcggtg tcccatctcc cacaacgatt aggatttttt ctggatttct
    15781 attttctccg ccattgtctt tatacaatgt ttcatagcca tttttaagcc ctagtgtaag
    15841 cggagttcct ccgattggtg tacgatttaa tgctgtattc tcacttcccg tcaaacgatt
    15901 gatatcattt gtcaatggaa agatgtccga actgatttgc tcatggttat tgatagctgt
    15961 actattatgg taaaaattca ccataccgat tcgaatatcc agactcgggt cagagagcag
    16021 atctttactc atttcgttta ctgcctcaat caacgcagca tttttagttt gactattggt
    16081 cccagcggtt aattcactca tactcgctga cttatctaga acaaaaacga tatcgattgg
    16141 actgatttcc tttttctcgc ccccgatgac atctagatag acattgtaaa gggttgttgc
    16201 atcttcagga tgtagatcag atgctgtgct gcttggcaga acaggttcta cccatttttt
    16261 gatgtacgcc ccgttataat ccaagtaact gttttcaaaa ttccgttcat ttccccaaag
    16321 ctgaacggtt tgagctggaa cgtttctatc ttcttcgcct gcagttgatc cattattagt
    16381 agcatagtcg taatttcgcc agttcgctcc cccgtttgta taatcactat catcgatcaa
    16441 gttctctccg tcatatggcg agcctttgtc tccttgatag cgttcattgc tttgtgtcca
    16501 atttcctaac acttttgtat agtcattcgt tgtaaacttc gggtagcgat gctgttcgtt
    16561 ttcaacttct ccgctaggat agatatattc ataggggtct tgcgtagctg ttggcccaag
    16621 acgctccaaa ataccgttga ataactggcg ttgaatgcgc tggagtgctt gaccgctggg
    16681 tacgattggt tcgtccagct tttccatctc attttcttcg ctacgttgaa tttcagcgga
    16741 atcttctgta tcagtttcat tttcttgtaa ttcttggtta tctattcttt catcaaccac
    16801 cacttgaaga ggtagttacc cttcgttcac atcggggatc ttttctgttt caaaaatcaa
    16861 tgtgccggtt tctgcttctt ttgaataaac ttttccgaca taccattctt gaccatcgat
    16921 cgtctcgact tttaggtctt ctccatcttc atcataggag gatagacctg ttccacgaac
    16981 gttttttacg gtaccaatcc cggcggctgc cgcatcgatt gctaaacgta ttctaccttc
    17041 attggcactt tccttttttt caaaagccag atgccattca agggtttctt ctttttcaat
    17101 atacttccct tcaatttttg catagtcctc atctaagaat acctcatcct cctttgtttc
    17161 tgttgactca gcagctacat ccagggtcgt catatgatag actgactgga aatatggtcc
    17221 aaatagcaaa ctgacagtta ctaactgcaa aagcttcaat tttaaccctt tcacatcctt
    17281 atactccttt caaaaatatt tgtcttcttt cacaacaatc aacaaaacaa tgcccatcta
    17341 attataattt aatttaattt taattttaaa tcaaatgata tccttttt
//
LOCUS       DAZKKW010000057        16956 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.058, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000057 DAZKKW010000000
VERSION     DAZKKW010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16956
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.058"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..610)
                     /locus_tag="ACG8PO_001831"
     CDS             complement(<1..610)
                     /locus_tag="ACG8PO_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFQ0207139.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYL"
     gene            complement(858..1694)
                     /locus_tag="ACG8PO_001832"
     CDS             complement(858..1694)
                     /locus_tag="ACG8PO_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HFQ0207140.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(1705..2634)
                     /locus_tag="ACG8PO_001833"
     CDS             complement(1705..2634)
                     /locus_tag="ACG8PO_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFQ0207141.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            2826..3179
                     /locus_tag="ACG8PO_001834"
     CDS             2826..3179
                     /locus_tag="ACG8PO_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFQ0207142.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(3253..3909)
                     /locus_tag="ACG8PO_001835"
     CDS             complement(3253..3909)
                     /locus_tag="ACG8PO_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HFQ0207143.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            4135..6069
                     /locus_tag="ACG8PO_001836"
     CDS             4135..6069
                     /locus_tag="ACG8PO_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFQ0207144.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(6155..7378)
                     /locus_tag="ACG8PO_001837"
     CDS             complement(6155..7378)
                     /locus_tag="ACG8PO_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFQ0207145.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(7372..9243)
                     /locus_tag="ACG8PO_001838"
     CDS             complement(7372..9243)
                     /locus_tag="ACG8PO_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HFQ0207146.1"
                     /translation="MKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQIDYIHQGVG
                     YGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYWGEIHERDQR
                     MVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQFFKIIVGTII
                     QKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYGLIYSVLEPE
                     KPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAMLWADLLPFKL
                     GEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLWLNKIFLLLA
                     LPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPNFHTLTNEKY
                     LKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKTYGQFIVRNN
                     VRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLYETGFAIECS
                     PEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLMTWENTFIPG
                     LEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKNNKKRLKLC"
     gene            complement(9264..10382)
                     /locus_tag="ACG8PO_001839"
     CDS             complement(9264..10382)
                     /locus_tag="ACG8PO_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFQ0207147.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(10465..11298)
                     /locus_tag="ACG8PO_001840"
     CDS             complement(10465..11298)
                     /locus_tag="ACG8PO_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0207148.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVSATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(11291..12208)
                     /locus_tag="ACG8PO_001841"
     CDS             complement(11291..12208)
                     /locus_tag="ACG8PO_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0207149.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(12435..13559)
                     /locus_tag="ACG8PO_001842"
     CDS             complement(12435..13559)
                     /locus_tag="ACG8PO_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFQ0207150.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            13742..14650
                     /locus_tag="ACG8PO_001843"
     CDS             13742..14650
                     /locus_tag="ACG8PO_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0207151.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPSKYYLSLK"
     gene            14791..16404
                     /locus_tag="ACG8PO_001844"
     CDS             14791..16404
                     /locus_tag="ACG8PO_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFQ0207152.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNNYTSARILAGLGIKAILIEE"
BASE COUNT         5352 a         3301 c         2804 g         5499 t
ORIGIN      
        1 ataaatactc atattcgagt gattttttat attttttaat tgtgccgttt acgagaatct
       61 cttcatcttt caatactagg cctattttat ttttataacc tggcatgctc attagataaa
      121 ggatttcatc gtaatggata tagtgatcgc tattaccata acggacagtt gtccaccttt
      181 ttttcgtagc atgttgaaca ggatagtttt ctttttcttt caataagtaa ctttggatgc
      241 tttttttatt tttttcataa gtgaattctt ttatcaaata accactaggc gtaatctttc
      301 gattaataac agttattgct ttattcggat gagacgttag ataaacaatt ttacactttt
      361 cattcttttc tctaagcatt tcgcctagtt cgataccgct ataataggcg ttcaaatcga
      421 tgtctaggaa aaaaatatct gaggaataga tttcattatt tttaatgaat tcaacaaatt
      481 gtataacgtt caatccctga acgatctcaa atttccaagg acttaataaa attctggatt
      541 cttccaaaat ttctactagc attttttcaa aaaccaaact atcatccaaa ataaatatac
      601 gcgttaccat aaaaagtacc tccactcgat attttactat caaaatcatt tcatacaact
      661 tttcaacata catgttagga ataaattgta attctcttca caatcttttc caacaccttt
      721 gaaaccgata tcaaatactt tcaggatgct gcttctaaaa taaatatcaa acaaatgaat
      781 acatcatgca aaaaaagcca gacaaccgac gtccttgcgt gtcagctgtc tgagctatgt
      841 ttatttattt tgttctttta aaaagaacga tacacatact gatttttcgg agcagagact
      901 ttctcagcgt ttgtaacaac tacagatggg atttcacgct tgaatggttg ataatagtct
      961 gattggatgg ttccttctat tttataccat tcatcatttt taaccgttac tccttccggc
     1021 atatgcgtca acaaaccaaa gactcctgaa tctgccacac aatggataat gccgaatcgg
     1081 aacaagaaga tatctgtatt gctatcgttc ggtgtttggt aaacaaatcc ttcatagctg
     1141 atttttttgc caatgaattc gctagggtaa ttataaatta gctccataac ttctaaatag
     1201 ttttcatctg tgatcttgat agtttgctta tccccatact tttctttcaa ctcattcatt
     1261 tggtctaagt agtctgattt gttgaaataa atactggtat ccggctttaa gtattgtgtc
     1321 tgcatctccg gatcccctac tgattcttta cttactggga aattaaatcc tttggcttct
     1381 acgatcgtag tgtcgagact aactgttgga aacaacgtcc ccacgattac tggcaaagct
     1441 agcagtacat aagcgattcc acgttgataa ggttttgaca acccatggtc atgatcgtgc
     1501 tcgtgtgcct tttcttctgc ttttccgttt ttgacccaca aatacagctg aactaaagcc
     1561 aaaagagcag ataaaatcat tgaaagaata gctaagtagc gataatggac attgatatat
     1621 tgatcgagtt tgccagaaac ttgcaagtac atcatcaatt cgaaataccc agataaaatc
     1681 aaaaaccgca gcatattttc cctcctaaat aaataatgtg aatccaataa cgctaagggc
     1741 aatcagtccg atcagcgata aaataaagcg tcctgtaaaa tatcgtttca tcatcagtag
     1801 atttttgata tcgaccattg ggccaaagac caaaaaggca gctacaggtg ctgtgccgaa
     1861 cagacttaac aaagaagatc ctataaacgc atctgcttct gagcaaagag acatcgtaaa
     1921 ggccaataac agcatgatga taatggctaa aatctttgtt gagccgagcg ttagcataat
     1981 tctagttggt aaataagtct gcatcgcagc tgcgattaat ccgccaatga tcaaataacg
     2041 acctgtatca aaaaattcgt cgattgaatg tgtcaacaca tgcccagttt gatggatcag
     2101 ctgatttttt ggttcattct tctcactttg ttcttcttca tggacatgat gatggtgatt
     2161 ttctgctact ttagacgtga ttttttcttt caatacagcg tctttttgaa aataggcaag
     2221 ccagacacca atcacaaggg caacaaaaaa gctgcctatc acccgccagc ttgccatctg
     2281 ccaagaattg ccaaatgcga taaaagtgga aaaaatcacg attggattga tgatcggagc
     2341 tgtcagcata aaagcaaacg ctgtgtgaac tggtacgcct tttttaacaa attgatgaac
     2401 gatcggtacg atgccgcatt cacatgaagg aaagaaaaat cctaagatcg aaccagtaaa
     2461 aatagagaag atacgatttt caggaagcca tctcttcaca cgttctgggg tcaaaaatac
     2521 ttgtaaggcg cctgagataa gacagcctaa taatacgaaa ggtaatgctt caataacgat
     2581 cgataagaaa atcgtactca tctgcaaaac cgaatttgga ataaaagaaa acatgagttt
     2641 cctccttttt atttctagtt cattcgcttt gcaaatatac gcttttattc aaaaaaaaca
     2701 agttgctctg taaattttta cacaactctt attttttacc aaagcacttc aattgacttt
     2761 ttctgttatt cccattaaat taaggtctca tacatatcac ttctatcata aaagggagaa
     2821 taaaaatgaa acaaaccact aacttgactg aagttcaaga catactgcaa cagtcatccg
     2881 ttgccgtcat ttatctttcg atgcctaatt gcagtgtttg tcacgctgtt cgtccacgac
     2941 tagaggaact tctcacacat tatgatattc cggcacttca tttggatgca tttgaaacac
     3001 cagaagtagc tagtaaattt gaagtgctga ctgcgccagt tgtcctgatt tttcatcaag
     3061 gaaaagaagt ctttcggcaa gcacggttca tcgactttaa aaaaatccgt tatctactag
     3121 atgaactgac agcagaagat gattcccttt cttatgaaga aatcttccga acaaaataat
     3181 cgttgtccaa acagaaaaaa gcgattgaac aagtgaaagt tttgccttca tttgttcaat
     3241 cgctttttat ttttattctg ctgtattttc ggaatgcgca tttgcgtcag aatgcaagaa
     3301 atagatcaag gtttgcaatt cattcgtcaa atcaacattt tggatcaata catctgacgg
     3361 agcattcaat cgagcagcgg taaagttcaa gattcctttg attcctgctt ccgttaactg
     3421 attgactact tcttgtgctc tttcagcggg tatcgttaaa attgctactt cgatttgttg
     3481 gatcttgatt tgttctgaca tatcttccat tggataaacg gggataccgt ctacgatgcg
     3541 cccaacgatt tcctcattca catcaaaagc acaactgata cgtatgctat tactttgatg
     3601 gaatttgtat tttagtaaag cacttcccaa gttccctaca ccgatcagcg caacatttgt
     3661 caattgatcg tcgtttaatg tctttgcaaa aaagtgcata agatcttcta catcataacc
     3721 atacccacgt tttcctaatt cgccaaaata agaaaaatct cgacggatcg ttgcactatc
     3781 tacttgaacg gcttcactta attcagttga agacactttt ttctttccag cattatttaa
     3841 aatccttaaa tagcgataat acaaaggcag tcttttagct gtcgcctttg gaataatatg
     3901 atctttcacg aaatgacctc cacgtattgt ctaattacat ctttgatttc tccatcatag
     3961 cagtatttat aggaaaaaat caattccatt gctcagatat tgtgaaattt tattctttat
     4021 tcatttattt atcatttctg caaatctcgc agtataatgg aatttatggt agactactaa
     4081 ttgaaaaata gttttttatt tgaggagaat tcacaaagta gaaaagagga ttccatgata
     4141 ttattacaag ccaatcagat tgctcgccat tttggtgcag aagtcttatt tgaaaacata
     4201 catttagaaa tcagtacagg cgctcgtgtc gctttagttg gcagaaatgg cgctggaaaa
     4261 tcgactttat taaagatcat tgctggaatt gaagcacctg atgaaggaaa gatcgctaaa
     4321 aataaaacag cgactctcgg atacctcgct caggacacag gacttgattc caatgaaaca
     4381 gtttggaacg agatgctgaa agcttttgaa caagttagac aaatggaaga gcggatgcgc
     4441 gagctagaga ttttcattag cgaaggtacg ccagacacac ctgcttatag ttcttattta
     4501 aaagaatacg accggctgca acacgatttt tcagatcgaa acggctatgg ttatgaaaat
     4561 gaaattcgtt ccgtattgca tgggtttaaa ttcgattctt cattttatga tcagaaaatc
     4621 gatactttat caggcggaca gcgcacacgt ttagctttag ctcgcatgct tttgcaacac
     4681 cctgacattt tgattctaga cgaaccaacg aaccatttag atatcgagac actttcctgg
     4741 cttgagaatt atctgcaaag ctatactggt gcattattga ttgtttccca tgaccgttat
     4801 tttttagata aagtagtcaa tgaagtctat gaattaagca gaagaaaaat gcgccattac
     4861 aaaggtaact attcgaaata tctagatttg aaagcagaac agttagcaag tgaatggaag
     4921 gcttatgaaa agcagcaaac agaaatccat aaattagaag acttcgttgc tcggaacctt
     4981 gttcgcgcct ctaccacaaa acgagcgcaa agccgcagaa agacattaga aaaaatggac
     5041 cggctggatc gtcctcaagg ggatgaaaaa caagcaaact ttctattcag tatagaaaag
     5101 ccttctggaa atgtagtctt gcaaatcgaa gatgccgcta tcggatatga taatgtcacc
     5161 ttatctgaac caatcaatct agatatccgt cgtcaagaag cgattgctct agtaggtccg
     5221 aatggtatcg ggaaatcgac gttgctgaaa tcaatcattc accagatccc ttttatccgt
     5281 ggaaaagaaa cgctagggac aaatgtcacc atcggctatt acgatcaagg tcaagctgat
     5341 ctgcatacaa acaagacgat cctagcagaa ttgtgggatg agcatcctac cactccagaa
     5401 aaagagatcc gaagtgcttt aggtgctttc ttattttctg gcgaagatgt agaaaaacca
     5461 atccctctgc taagtggcgg tgaaaaagca cgggttgctt tagccaagtt agcgatgaac
     5521 aaagaaaatt ttttgatttt ggatgaacca acaaaccatt tagatattga caacaaagaa
     5581 gtattagaaa atgcattgat cgattatgac ggcacactgc tttttgtttc ccatgaccga
     5641 tatttcatca atcggatcgc gacaaaagtc gtcgaactct ctgaaaatgg cagcaaagtc
     5701 tatctaggag actatgatta ttatttagag aaaaagcagg aagaagaaga aatcgccgaa
     5761 ttgttggcac aagatgagcc agaatctgct cctgctccca cgaacaaatc tgattattac
     5821 caaagcaaag aacagcaaaa gcttatccgt agccttcaac gaaaaattac tcaggttgaa
     5881 gaagaaatgg cgcggatcga tgaatggatc gatcagttgg aacaagaaat ggtcgcgcca
     5941 gaaaacctgg aagatcacgt aaaactcaat gagctgaacc aagatttaga agctgctaga
     6001 caagatcaag aaacaaaatt gacggaatgg gaagaactaa gcttggaatt agaagaatat
     6061 gaaaattaac gaaaaaacca aaacagaaac cattcatgct gttttggttt ttcttttttt
     6121 attttatcaa acaaaaattc catatttacc tttttcacca ataagaagac caattactag
     6181 tcattctcac taaagcttct aaataatagt aatcacccca aatacaaggt gtatttaccc
     6241 cattattttt gggcttatta taaacagact gtgttaaaaa gccatcttct tcttccgttg
     6301 tagcatatct ttctttcaat tgtcgcataa ttttccaaac agcagactgg tataagttct
     6361 tttctgtttt gtctaataga cctgattcta ttaattctaa taatccacaa accatgatca
     6421 ccatactaga tgtatctttt tcttctctgc tagaatcatc aaagatcaaa tcccaataac
     6481 aaatggtatc ttctggcaac cgattcataa aataattggc taatattttg cttaaatcaa
     6541 aatatttttg ttctttcaaa taacgataac ttaatgcaaa accgtaaatc ccccatgctt
     6601 ggcctctagc ccaacaggaa ttatcactat aaccttgatg cgtacttcca tatcttggtt
     6661 ttccatcttt gacatccata taaaatgtat gataagttga accatcttct cttactaaat
     6721 aatctgctgc attttgaata tgagcctgag cagctttttt atatttttga tttccagttt
     6781 cttcactcgc ccaatacaat aatggaagat tcatgcaaca atccatgatc attctacctt
     6841 gttgtgaagg atcactagga tctccccaag cttgtataac tcctgccttc tcccaaaaac
     6901 gctgaagtaa tgtttctgca gcatcaattg aagcttcctt catttttcga tttcccgtta
     6961 ttttataatc tgctacggtt gataaagaat acaaaaaacc aagatcgtga tgattagtga
     7021 taatttgaca gtcaatcctg tggataaaat ctatatctgc tttttcagct gtttgttgaa
     7081 ataaaggatc acccgtcttc tcaaataaca accaattaat tcctgtccaa aatccttctg
     7141 tccaaccaat attgtctctt aatggataac gtccttttat aggacactcg cttgggaatt
     7201 gatactgact gttactgata cgtttgatta acctctccgt gtgtctgcga caacgctcga
     7261 tctcattatt ccattctttt tccattaatt ctttataaca ttcatttatc catttttctg
     7321 gatgtattat ctcttcaaac atcactttat caagcatttc cccaccagcc tttagcataa
     7381 ttttagtctt tttttattgt ttttattttg gatagttagc ccggttgtcc catcaaaatc
     7441 aattttggga cgattgttcc atacttctaa actataatcc cgatcttgat gagcaaccac
     7501 tcctgttccg agccaatggg ttccttttcc aaatgtaccc tctaatccag gaataaaagt
     7561 gttttcccat gtcatcaagt ttgtattggg caaacccata atagctgtgg attttcgttt
     7621 aatagtatcg tcacttaaac agataatacc tgaaaagcca gcttcactca ccctatagtt
     7681 ttccttgtct tcttctatga ttattccttc tttctcagga gaacattcaa tagcaaaccc
     7741 tgtctcatat agaacatatt ctgtatctaa ttctactttg tgtacgcgaa tctgccagcc
     7801 ttcaaaagga ataagccaag ttctgatttt tgcctgtaaa gtaggttccc atgtagaggc
     7861 aagtacctct ttcttacata caatgtctcg tacattattt cgaacgatga actgtccata
     7921 ggttttgatg ctctcattct taaaactggt cgtgatatat gtgtccgctc gctgaatccc
     7981 tagcattgaa tccattgcca tttctgcttt caattgattc gctcgcggta tcgaaaaacc
     8041 aaattgacta gaataagcaa attttaaata tttttcattg gtcaatgtat ggaaatttgg
     8101 ccctaattgt cccgcattca aaaaaaatgt atgtccatta tcatgttgta tgagcaaatt
     8161 ggcttctgaa agaagtttag ttccttcacg aataggcata ggcatttctt tagccgtcca
     8221 atactcatgg tcttcaggta gcgccaacaa taaaaaaatc ttattcagcc ataatggaga
     8281 taacgtacta ttatagggtt cagtcaatgt caattgaggg taggtataac caatattcag
     8341 gatacctttt tcatcaaata tttctttttg catccaccat cttatattcc gattgactaa
     8401 ccccttgatt tcacctaatt taaaaggcaa aagatcggcc cacaacatag ctgaccaaaa
     8461 gcttgaaact gcaaaacgat aaatcatgct tcgaccaaaa ggcacatttg caccatcttc
     8521 cgcaaaaaaa tgaatatagt ctctagaaaa atcaatagct cggttcttat atatctggct
     8581 aatgggcttt tctggttcta gtacgctata aatcaaacca taataatgaa atgcaaaagg
     8641 attataataa tcttggcgac ctcttgaact gtctcgatac cagccatttt gaagataaca
     8701 attttctata ttatcaaatg cttctttcag tgctgattct ttaactggat aaccaaattt
     8761 ttggataatt gttccgacaa ttatcttaaa aaattgccaa ttaccatctg cacattcata
     8821 ttcaaaaatt tgcgagaacc aatgaataat ctggcttttt tctcctgtat ctaatttttt
     8881 ccataataat tgttcatgat gatataacgc taataacatt gcgggcattt ctaccatacg
     8941 ctggtctctt tcatgtattt ctccccaata atcgggatgg gaaggatctg ttccagcttt
     9001 tattcctgat agaattaatt tcaaccatcg ttcatctaat tggtcaattg cgggccccgc
     9061 tccccataaa acccgagaga atccttccat tcctgcaatt ttatttccat atcctacccc
     9121 ttgatgtata taatcaattt gtgcgccact tgctcgataa aacggctgga gtggttgatt
     9181 tatcttttta aataagtcaa agtaatcctg tctggtcctt aatagtggta ctggatgttt
     9241 cattgtattt tctcctcttt ttactaccaa atagctaatt ccgactgttc taatcgaagt
     9301 aagccttcaa taaaaaaata atcagaatag atcagtggca cattataagt cagttctcta
     9361 tggtatgctt ctgctccgcc tccaatgatc ccatcttcat ccatattcca atttgcatag
     9421 tgcttttctg tcttttggag tatattgata gcagcttcat gatacatttt tgatcgtaca
     9481 gaggaaacat atcctgctaa ttccagaaga ccgcatgcag cgcatactcc agcactggta
     9541 tcaatattcg aatcttttac agcaaaatca atgagaggaa tataaccagt tttttcgatt
     9601 ttctgaataa aatttactgc tatcttttct gctatagcta gatagtacgg attttttgtg
     9661 taacgataac ttaacgcgaa tccatataaa gcccatgcat ttcctctgct ccaacaagaa
     9721 ttctcattgt atccttgccc gccaagtaac cgaatatatt tccctgattc tgcatcaaat
     9781 tctgcgatat gcccggttga tccatctttc ctcactagat acttagctgt tgtatctgca
     9841 tgctgccgag caattttaga gaacctcgga tcaccagttt ccctcgaagc ccaatacagt
     9901 aatggtaaat tcatcatact atcaataatc atccaaccag gtttatggtc attccatgct
     9961 actaaatagt tccctgaaat attatagcgt ccagcaagta ccgtagctgc atgtaaccct
    10021 agttgtcgac tatgtttgtt ttgagactgt tgataatgtg caatagccga tggtaagaat
    10081 aaaaaaccta catcatgatg aacgtctgaa aaatggtaca gggcagattc taataattgt
    10141 tcttgttgta ttgctgcctc tttgaacttt tctcgttttg tcttgtgaaa gagctgccat
    10201 aaaataccac tccaaaaacc attggtccac cagtcgattc ctctgtcctt tattacgtct
    10261 gtatattttc cgttgtaaga aatatatgga attttattcc ccattctgtc taattccgca
    10321 tctatctttt cagaagctac attcaaaatt tttcttgacc attccttcgt ctgttcattc
    10381 atggatatcc cgccattctt ttaattttta tgaatgtgga gttgctccta gaacgaacta
    10441 gatggttaac attcgttatt ttttttactt atttgcagct ccaatgtcat tctccttaaa
    10501 tttagactgc gcccacacat aaatcaagac cattgggatc actgaagttg ctaaaattag
    10561 cataattgcg ccataattaa ttggctcacc tgtactagaa accgtcatat ttttgataat
    10621 taagggaacc gtatatttat ctttatctga aagcatgact aatggaaata gatattgatt
    10681 ccaacttccc ataaagataa gtaaaccact agtaataatc gtaggtgata aaatcggtac
    10741 agcgattttc gtaaaaatca cccactcatt ggcaccatcg attctggcag cctccatcag
    10801 tgctgtagga aattgactgc tatattgtcg catcataaac actgaagttg tactggcaat
    10861 cgaagggatg atcacacctg cataagtatt cgataatcct aaacgactca taatgtaaaa
    10921 taaaggaatc aatgtggtaa aaaaaggcac catcctagat agcataactg tattgaacaa
    10981 cagtttattc cccataaaac gatactttgc taatgcataa cccgccattg tagaactggc
    11041 tgtagctgaa actgtactga ttacagcaat aacaatcgaa ttccaaataa tcaagggata
    11101 attatatctc gccatgatgg cttgccagtt ttttaatagc atatccccaa acttgaaatt
    11161 gattccgcca cttaaaattt cttgattact ttgcgttgaa gctacaatca tatataaata
    11221 tggcatcaaa aatattaagg caccaaacac aataatgacg tacgtaaaaa ttctgctttg
    11281 tatcttttgt ttagtcattt ttatcacctc cgaatttaaa ttgaatagcc gataatataa
    11341 cagatacaaa caccatcgtc caggcaatag cagaggcttt tccaaaatca accatttcaa
    11401 aacttgtttt atataaattg actcctacga ccaaagctga attttctggt ccattggaag
    11461 tcccaaagaa tatatttggg atattgaaat tacccaagcc attgatagtg gctaaaactg
    11521 tggccattaa aatgattggc tttagcatgg ggatcgttaa taaaaagaat tgccgaaatt
    11581 tcgatgctcc atctatttca gcactttcgt atatctcttt agaaatccct tgaagaccag
    11641 ctaaaaataa aatagtgtta tatcctacac taagccaaat catcgtgatg atcattgata
    11701 ttcgtatcca aaaaggattt gttaaccact gtacaccgtt aattcctagc gcattcaaaa
    11761 atccattgaa taacccagtc ggattgaaaa aagtcatgaa tacggaagct acagccaccg
    11821 ttgatgtaat ggttggaata taatagacag ttctaaaaaa tccttttcct tttaactgtg
    11881 ctgaattcaa taaaacagca aagtaaatag ctaatagcat cgttattgga attgatcctg
    11941 ccatgataat caaaacattc gtgtaagatt taataaacaa tggatctcgg aagatagcta
    12001 gataattgtc aaatccaaca aattcaaatg agtagccaat tttcataaaa ctaaaagcaa
    12061 acgagataat gatgggaatc aaaatcgtca caataaagaa aataacaaaa ggtgtgacca
    12121 atccataagc acttttttta tgagacgtat ctgttgcttt tttcttgctg ttctttattg
    12181 tcttgcttgg taacgaagat tgcatcatcg ttaagtcctc ctaatatttt ttaatcacca
    12241 gttaatttta cgccgtactt atgagcaaaa ttttctgctt cttttagata tgaattttca
    12301 aaatcacctg tttgccaata gttatatgca gcggtagtca aataagacgt agcatctgaa
    12361 taagtggctg gtaaaacagt atttgatgta ttttcattga ttttattcaa tagttcatag
    12421 tatttttgtc ctccttagta gtcaaacttt ttctcagctg cttctgtttg ataggccaat
    12481 gagttagatg tagccacgcc cagctccagt gcgttggcta agcattcttc atctgtcagc
    12541 ataaatgtaa gaaattgttg tgccacttgt ggatttttgg catctttagg gacataaaaa
    12601 cttgatcctc ctgatactgc accttttcct ggattttctt ttgaaaaagg aatcagtgga
    12661 gccatgcgcc atttgtcaga tgcatcagga aaattcaaag acatattcgt agcataccag
    12721 ccgccagcaa caaacattgc agattcttga aatgctttct cagcatcttg tgcaccgtag
    12781 aaatgtacaa tatctgagtc gatcatttct ttgattaact ttgcagcatg tttcgcttct
    12841 ttagtattca gacggatatt tccttcttca tcaagcaaag attgattctg ctgggctagc
    12901 atgttgataa acaattcttg gtccccagat tctggcaagg caattccaaa actaccagtt
    12961 ttttctttta atgcttttgc cacctggata tattcttccc aagaactgat ttcttcaaaa
    13021 tctacaccta tttcagcaaa tttttttggt tgataataac aaagtgctgg ggtaaaatca
    13081 ctagcccaag gaataatcgt ttcattactc gtttgttgac gcaaaatatt caatttttga
    13141 tcataaaact gatcgccata tttgtcataa aatccatatt catcagcact ataaaaagca
    13201 tcttgaaatt tttccagcac acctgctgct gaagtgtctg ccatggagat aatatccggc
    13261 atatcttcgt gtgttaccaa ttttggggtg atcatttgta ctgttgctgt tcccgaagct
    13321 acattcacaa tttttaagtc aatatttgct ccagttttgc ggttatagat ttttgcagca
    13381 ttttcaacat agtttgtcag tttgccgttt gcgacatata cttctaatgt agtggctttt
    13441 tttatcattg gatctgcgat gtttgctgtt acttcttcgt tagtttttcc acatgcactt
    13501 aagctgaata atagggtaat gtttagcaaa gctatcacca atttgtatct ttttttcatg
    13561 tctatccctt ctttcttttc aagttcttat ataaaacgct ttcacttatg tatattaacg
    13621 gaattagtaa cacaaatatt tatataaaaa aatattttac ttgtcgtttt ggcttttcat
    13681 ttttgttata atagtcatta attataaata gaaagctttt tatataaaaa gagaaaggaa
    13741 agtggttctt ttgttaacaa acaaccaatc ctccaatctt gtcaaaaaaa cactaaccta
    13801 ttccatagat acccatcttc atttatctgc tgctccctat tttcgactac taataaatga
    13861 aagcgctaat ttatatgctg aatttgcaca taaatctata gaactagata atccttccct
    13921 tttattcatt gataaacagc aagaagtcca aatcttttct actcaaaagg aaacacaaac
    13981 ttttatcatt atccagtttt attttgatga gcaattatta gcacatatta cgggacaatc
    14041 tttttctata gatcaagaaa taacatctat cattactttt ctgaaaaatg aacagttcaa
    14101 aattgattat ctaaaacata gtaagccagc tatttcctat gatcaggaaa tcaattcttt
    14161 tttcatacta tgccaacaga tgagcattca tttgctttcc ttattagtta cttattgtct
    14221 gcaaaaaatt caattgtctt ccttaccaag gcattatcaa caggaacttg ctttattgac
    14281 aataccttca aatgagaaag tcattcctaa aaaaaagcat attctttatt cagatgaatt
    14341 agttgatcca atcatacgat atttagaaga acatctttca gaaaattttt tgcttgaaca
    14401 attagcccaa atttttttta tcagtccagc cagcttaaaa aaacatttta gaaaagtgaa
    14461 tggaaaaagt gttattagct attttaaaga actaaaaatg gaaaaagcaa aacgtctcgt
    14521 gctagaaaat gaattaacct atacagaaat tgcagatata ttaggatttc attcgatcca
    14581 tcatttttct tcagctttta aaaaatatac agggttatcc ccttcaaagt attatctatc
    14641 cttaaaatag aaaaaaattt ttaggtttta tccacaaatt atttctctat attttctgtc
    14701 tattcaaaat aaacaataat tttccggcac aattatttgc tgttttttac cataacatag
    14761 tacgatcaac gtagaaaagg agatgttttt atgaaagttg ttgtcattgg cggtgtagct
    14821 ggcggtcctt catttgccac tcgtttccgt cgattgaatg aagcgcacga aatcattatc
    14881 tatgaacgcg gagaaaatat ttcttatgca agttgtgctt tgccttatta tttaggaggt
    14941 gtgatcactg accgtgactc gctgatcgaa cgtacaccag aaatattgaa aacaaaaaac
    15001 aacatcgatg tctttactaa acacgaagta acagcaatcg atccttctac taagcgatta
    15061 acagttaaag acctatccac aaatgaagaa ataaaaacag attacgataa gttgatcatc
    15121 tcttctggtg ctagaccaga ttatccggat attcccggag tttttgaagc agaaaacggc
    15181 tttgtcctcc gtagtgtgac ggatgcggat cgaatcaaat cgttccttga agaaaaaaat
    15241 ccacaacatg tcgtcattct tggtgcaggt gttatgggtc tggaattagc tgagaatttc
    15301 aagcatcgcg gcttagacgt gactttaatc gatcaattgc cacaagtcgc tttcccttat
    15361 gatccagaaa ttgctaattt agtttatgac aaattgctga aagaaggatt agccgttcat
    15421 ttagaaacaa gagttactga gatccgtgat aaaggtcgag aaatagtatt atcagatggc
    15481 tccgtccttt ctgctgatat gctaattttt gctgttggtg tttctccgaa gaatgaagtg
    15541 gtgaaagcaa ccggcataca attatcagat acaggtcaga tcattgttga tgaccagtta
    15601 caaaccaatc tgccggacat ctatgcgatt ggcgatatta tcgaaacgac tagtgtagtg
    15661 actggtcagc cgatccaaag tatgctttcc agtgcggcca atcgtcaagg acatatgctg
    15721 gcagatattt taaatggtac gcctatgcgc tatcgcggat atattggtgc aggtgtcgca
    15781 aaaatctttg atcatacagc aagttatgcg ggaatgacag aacatgaatt aaaagcatca
    15841 ggcataacaa attataaaac tgtttttatc actccctttg accatgccta tttctatcca
    15901 gaagctacaa gattaaatct aaagctgatt tttgatgcag atagcggtcg tattttaggt
    15961 ggacaagcat ttggagaaaa aggtgtcgat aaacggatgg gagaactttc tgtagcaatc
    16021 accggaaact tgacagtctt tgatttgcct gatttggagt tgccttactc tccaccgtat
    16081 tctactaccc gtgacccgtt gaatatagct ggttatgtcg caatcaatca aatgacgaat
    16141 atcgtagaaa cgatcaaagc aagtgatata cccgaaaacg atttgaaaga agcgttcttt
    16201 ttagatatac gtgaacctaa taaagcacct tctggcagta tctcggcaac aaaaaatatt
    16261 ccaatgaatg agttacgaga tcgaatcaat gaaatcccaa aagataaaaa agtttacatt
    16321 actttcagaa gaggattgaa taattatact tctgcccgaa tcttggcagg tttgggtatc
    16381 aaagcgattt tgattgaaga ataaaaaaac gaccgcggtg ggtagccgcg gtctaggagt
    16441 tatttttact tgaggagtaa aaatgaaaaa gtatataggg ttgttggttg gtatgtatat
    16501 agaatagaag ataaatgtga agaaattgtg cattatttct tcacatttat taggttatag
    16561 tttaagaaag tttaaacatt gcgcaattta attattttag ctgtttcttt tcataaaaaa
    16621 acatgttgtg actctgagac aaatcatagt ccatagtgat gtctctagag taactacgcc
    16681 taatattacg aggcatcata ttaatctatc agcactagta cctaaactag catccctttc
    16741 gaatattttt tcttatataa taaagtttgc tttcactcct ctaatctcac caaatcgact
    16801 ggcttgcgtt gtgtgatact aggaactgat tggtttagaa cgacttttct attgtaatca
    16861 tatttttcgc catcctcgtt gtcttctaat ccttcaatga atgatcccta atgtgtgtat
    16921 taaataattt aatttttttg ttcctattta tttaac
//
LOCUS       DAZKKW010000058        16786 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.059, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000058 DAZKKW010000000
VERSION     DAZKKW010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16786
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.059"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(39..848)
                     /locus_tag="ACG8PO_001845"
     CDS             complement(39..848)
                     /locus_tag="ACG8PO_001845"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFQ0207153.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
     gene            1141..2577
                     /locus_tag="ACG8PO_001846"
     CDS             1141..2577
                     /locus_tag="ACG8PO_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFQ0207154.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            2747..3943
                     /locus_tag="ACG8PO_001847"
     CDS             2747..3943
                     /locus_tag="ACG8PO_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HFQ0207155.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(4005..4190)
                     /locus_tag="ACG8PO_001848"
     CDS             complement(4005..4190)
                     /locus_tag="ACG8PO_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HFQ0207156.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            4421..7735
                     /gene="dnaE"
                     /locus_tag="ACG8PO_001849"
     CDS             4421..7735
                     /gene="dnaE"
                     /locus_tag="ACG8PO_001849"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFQ0207157.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KRQLIYLLGEDNVVFK"
     gene            7903..8865
                     /gene="pfkA"
                     /locus_tag="ACG8PO_001850"
     CDS             7903..8865
                     /gene="pfkA"
                     /locus_tag="ACG8PO_001850"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HFQ0207158.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            8939..10723
                     /gene="pyk"
                     /locus_tag="ACG8PO_001851"
     CDS             8939..10723
                     /gene="pyk"
                     /locus_tag="ACG8PO_001851"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFQ0207159.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(10758..11660)
                     /locus_tag="ACG8PO_001852"
     CDS             complement(10758..11660)
                     /locus_tag="ACG8PO_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFQ0207160.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            11846..12415
                     /locus_tag="ACG8PO_001853"
     CDS             11846..12415
                     /locus_tag="ACG8PO_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HFQ0207161.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            12473..12652
                     /gene="rpmF"
                     /locus_tag="ACG8PO_001854"
     CDS             12473..12652
                     /gene="rpmF"
                     /locus_tag="ACG8PO_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFQ0207162.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            12822..14243
                     /gene="gndA"
                     /locus_tag="ACG8PO_001855"
     CDS             12822..14243
                     /gene="gndA"
                     /locus_tag="ACG8PO_001855"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HFQ0207163.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDEAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
     gene            14436..15122
                     /locus_tag="ACG8PO_001856"
     CDS             14436..15122
                     /locus_tag="ACG8PO_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFQ0207164.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            15119..16624
                     /locus_tag="ACG8PO_001857"
     CDS             15119..16624
                     /locus_tag="ACG8PO_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFQ0207165.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
BASE COUNT         5397 a         2942 c         3529 g         4918 t
ORIGIN      
        1 attgtcacaa cctctttttt gtggaatcta attctctatt atagtttaag tagtttttcc
       61 agataaacag ctaaaccgtc ttcctgattg ctcaaaggag tgacatcatt tgcaatgcct
      121 ttgagttggt cagtaccgtt agccatagct actccccaac ctgcataatc caacaattcc
      181 aaatcattat gctcgtcacc aaaggcaatg acgtcttttt gatcgataga caggtagtta
      241 gcaatctctt gcacgccttt tgctttttgg attccttttg aaacaatctc taagatggca
      301 gtaggtccac cccatgtccg aacatcgaca taactgccaa attgacgtgt taattcagca
      361 gatacagctc ctgcaaaacg tttgtctgtt cggacaagca aagatgtagg atttgtcgtc
      421 aaatttttag gagaaagcaa attcttctct cctggacgag aagaagcaaa aattttttca
      481 tcaaagaaat caaaactgtc aataaaaaaa gtatcacgat tttcagcagc gataaaatct
      541 aaatttagct gctttttttg cgataagatt tcaaaagcga tttcacgatt gatcaacgtt
      601 tctctttcac catcccagtg ctgattaggg atgtgtacta atgcgccgtt aaaattaacc
      661 atcggtgttg ttaattctaa ttgttggtaa aactgatggc tcattcggaa tgggcgtcct
      721 gtagcaatac tgacatgatg accggcattg attgcttttt ttagagtctc ttttgtttta
      781 gatgtaatca tcgaatcttg attgagggta gttccgtcca gatcgatggc aattaatttt
      841 ttattcaata agggtccttc cttcctcaac tagcatttgt tttagtctag catattttag
      901 gtaattaaca agagcaatca gctattccta acaaaaacta cagttatggc tatataaata
      961 gctaaacata agctaaacac gaacgttaag tattattttc gctttttttg ttaaaaaaac
     1021 gttgaaaagc gaatgaaacg tctatataat ttgaaattgt tcgatggatt tgccctattt
     1081 tacttatttt ttattattat agttttccat cagaaaaaaa ttcagggagt ggaacttttt
     1141 atggaaaagt ttttcggttt aaagaaaaac aatacaaatg tatcaactga aataatggcg
     1201 ggagtaacga cattctttgc catgagttat atcttattcg taaatccgac gattttgtca
     1261 gcatcaggta tgcccttcca agcagtattt ctagcgacaa tcattgcatc gattattggt
     1321 acgctggtaa tgggattgtt cgcaaatgta ccatatgctc aggcacctgg tatgggattg
     1381 aacgcatttt tcacttttac ggtcgttttt gggcttggtt attcttggca acaagccttg
     1441 gcaatggtgt ttatttgcgg attaatcaat atttttatta ctgtcacaaa tattcgaaaa
     1501 atgattattc gggcgattcc cgaaagtctt caacatgcga ttggtggcgg gatcggtatt
     1561 ttcgttgcgt atgtcggaat caaaaatgca ggattcttat ctttttcagc agaccagtca
     1621 gctatttcga gctctgtcgt tgaaggcgga aaagcgacaa atgttacgat aaatggtgga
     1681 atcgtccctg cgttagccaa ctttgacaac gctcctatct tattggctgt gattggtttg
     1741 gtactgacag ccattttagt tgtaaaaaat gtccgtgggg ctattcttat cggtatcgta
     1801 gcgaccacag tccttggtat ttttatgggt gttgttgatt taagttccat tgattggcaa
     1861 acgaattcgt taggtaattc atttaaagaa ttaggaacaa ctttcggtgc agcatttggt
     1921 tctgaaggca tgcaatcttt attcagtgat tcttccaaga ttcctcaagt attgatgacg
     1981 atcatcgcat tcagtttatc tgatactttc gatacgatcg gaacatttat cggtacagga
     2041 cgccgcacag gtattttctc aaaagaagac gagatcgcgt tggaagatgg tcgtgggttc
     2101 aaaacaaaaa tggataaagc actatttgcc gatgcgatcg ctacttctgt gggggctatc
     2161 tttggtacat cgaatacaac aacttatgtc gaatctgcag ctgggatcgg ggccggtgga
     2221 cgtactggtt tgacttcagt agtagtggca gtgctattcg cattaagtag tttattctct
     2281 ccactgatcg ccattgtacc aaatcaagca actgcgccag cattgatttt agttggagta
     2341 atgatgatgg cttcttttgc agacatcaaa tggctagata tggaagaagc tttaccggcc
     2401 ttctttgcat cgatctttat gggattgtgc tatagcattt cctatgggat tgcagcagga
     2461 tttattttct atacaattgt gaaagtaatc aaaggaaaat acaatcaagt ttctgttgct
     2521 ttgtggatcg ttgatatttt attcattatc aacttcatca ttctagcaac aatttaacta
     2581 aagagaaggg attggaacag tatttgttct gatctctttt tgttttacat aaaaagaacg
     2641 ttacatataa aatgaccttt gcgggatttt tctcttttta ctggcgagat cgttgcttgt
     2701 ttattcgaaa ataggtatga taaaagagac gaaagaggga tgatagatgg agattaattg
     2761 gagaaaaaat ttagtgattg cgtggattgg ctgttttttc actggtgcaa gtattagttt
     2821 agttatgcca ttcataccag tttatgttga gcaactagga acaccaaaag atcaaatcga
     2881 attgttttcg ggactagcaa tctctgtgac tgcttttgca tctgccgtgg ttgcaccaat
     2941 ctggggaaat ctagctgatc gaaaaggccg aaaactcatg atgatccgtg cagcagcagg
     3001 gatgacatta acaatgggat ctttggcttt tgtaccaaac gcgtattggc ttttgatcat
     3061 gcggttttgg aatgggattt tgtctggata tattccaaat gccacagcga tgattgcttc
     3121 acaggcacct agagaaaaga gcggatgggc gttaggaaca ttgtctaccg gagcgattgc
     3181 tggaaatctg atcggacctt caatgggcgg tgcactggct cagtggtttg gtatggaaaa
     3241 tgtattcttg atcacagggg ctttgttgat gatcacaacg gtattgacca ttttcttagt
     3301 caaagaagat ttccatccaa tcgagaaaaa agatctgata agcaccaaag aaattttttc
     3361 aaaaatggat catctctcta ttttgatcgg tctcttcatt acgaccttga ttttacagat
     3421 aggaatcact acgatcagcc ctattttgac cttatatatt cgtgaattga gcggaagtac
     3481 agaaaatatc ctttttgtca gcggcttgat tgtttcgata gctggtgttt ccgctgtctt
     3541 ttcttcaccg aaactaggaa aactagggga taagattggg aatcaaaaag ttctaattgg
     3601 aggactcatt ttatcatttt gctgttattt acctatggca ttcgtcacta caccgctcca
     3661 attaggtata cttcgcttta ttttaggctt ttcaactggc gctttgatgc cttctgtcaa
     3721 tacactgatc agtaagatta cgcctcaaga aggagtcagc cgaatctata gctacaatca
     3781 gatgtttagc aactttggac aagttttagg tcccatgcta ggatcgacag tcgcgcatgc
     3841 ttataattac agtgctgttt ttattgtgac aagtttgttt gttctgtcaa atattcttct
     3901 ttctttattc aactttcgta aagttcttcg cagcagatta taatggtggt gaggagtaga
     3961 aaataaaaaa gagcttgaga aatctcaagc tcttggctgt ttttttacga atgggcagag
     4021 gtttcttttc ttggcgcaac tgcaccaatc aaaaggataa tgacacccgc aatgaatgaa
     4081 atgattgttg tcaaagtaaa atcatatgtt ccgcttgtta atgcagcacc aatataaccg
     4141 actacttgac ctagaacgac agcccagaat aatgtaacga tatatcgcat aaagaacacc
     4201 tcttttctat cttaatttta ccagattttc tttgaatgta aagaaacgag accaaatctc
     4261 aataaaattt gagttcttgc gaaagaactc attcctacag attacaagca agtaaatgaa
     4321 gtggaataat caacttcagc gcctgctatc ttaatgaaag taaaaggatc aaacctttta
     4381 acgctagtga attttaacga aaaaaaaggc ggaatgcaag atgtttttcc cacaacttta
     4441 tacaattact tcttattctc ttttacaaag tacgatacgt attcgagaat atgtacaaga
     4501 agcaaaaaaa caaggctaca cttcattagc cataacggat aaagatgtct tatacggtgc
     4561 acctgaattc tatcgtattt gtgtagaaga aggagtacag ccaatcattg gattacatct
     4621 tagttatact tatgaagaaa aaaattatgg cttgcttgcg tacgcaaaag atgaaatagg
     4681 ttatcagcag ctgatgaaac tttccagccg aaaaatgatc caagaaaaat tggaacttgc
     4741 agaaatgaca gaaaccgacc atctagcgtt tgttgttccg gcaaatcatt ctgtttctga
     4801 ggctttcaat aaagaggata actttttcgg tcattggcag tttttacttg aaaatgtttt
     4861 tactagaaat ttttatatcg gcttgacaga aaacacgcct gatcaattgc ctgaatggta
     4921 tcagtatgtg ataaaagaaa attattcgct ttgtgcatta catcccatcg ctagtttaca
     4981 acaagaagaa ctttttgcac ttgaagtgat gaaacatatc aaagagggaa gccaaatacc
     5041 tttagaaaat ttatctgcat ctttaccact aacagcaaat gtctatcttc tttcagaaca
     5101 agaaatgatc aaacgattcg aaaaagcggg tctttcccaa gctgtaatag gcgcccagcg
     5161 tttagctgaa tcgatttcat ttgaattcca gttccatcag aaattacttc cgcattatcc
     5221 agttcctaat ggaaaacaag ctggagaata tttaagagag ttatgctatt cgcaagtgga
     5281 acgccgaatc actgactata caaaggaata ccaagaacgt ttggactacg agttatctgt
     5341 catccatcaa atgggattcg atgattattt cttgatcgta tgggatgtaa tggcttttgc
     5401 tcatagaaac aaaatcgtta ccggtgcagg tcgtggttct gctgcgggtt cattagtagc
     5461 ctatgtgtta gaaatcacag atgtggatcc attagaatat gatttgctgt tcgaacgctt
     5521 tttaaacccc gaacgcttta cgatgccaga tatcgatttg gatattcctg acaatcgacg
     5581 tgaagaagta ttgaattatg ttcgagaaaa gtatggacaa tatcatatgg cacaaatcgc
     5641 aacatttggc acaatggcag caaaaatggt tcttagagat gcagctagag tgtttggttt
     5701 atcgcaaagt gaagcaaacc gctggtcaaa agcaataccc aatcaattga aaatcacatt
     5761 gacccaagct tataaaactt cgcatcaatt agtagaatta gttcaggcaa gcgaaaaaaa
     5821 ccaactttta ttcaaaactg cagtgatttt agaaggattg ccaagacatg tttcgactca
     5881 cgcagcaggt gtagttatca gcgataagaa tctacttgaa ctagttccgc tccaacgcgg
     5941 ttctgatgaa gcttttctga cccagtatac gatgaatgag gtagaagcaa tcggtctttt
     6001 gaaaatggac tttttaggac tacgcaactt atcgattatt gattttactt tgaaagctgt
     6061 taagcgagta gaagggtatg agatccgatt aaaagagatc ccgctaaatg atgcaaaaac
     6121 gctgctcttg tttcaacgag gcgaaacctc aggagtattt caatttgaat ctgctggtat
     6181 ccggaacgtt ttacgaagat tagatccaga aaacatcgaa gatattgccg cagtcaatgc
     6241 attgtatcga ccaggcccta tgcaaaatat agataccttc attgccagga agaaaggaaa
     6301 agaagccatt cgctacccag atgattcact gatcccgatt ttgaaaaaca catatggagt
     6361 gatcgtctat caggaacaaa tcatgcagat cgcctcgact atggctggat ttacattagg
     6421 gcagtcagat attctgcgac gggcagtgag taaaaagaaa aaagacgtat tagatgaaga
     6481 aaagtcgcat tttgtatcag gagcattaca acagggatat tcggaaaaaa cagcagcgac
     6541 cgtatatgaa tatatcgaaa aatttgcaaa ctatggattc aatcggtcac atgcatttgc
     6601 ttattcattc gtgggattcc aaatggcgta tctaaaagcc catcatccag gtgctttctt
     6661 taccagcctg atgaattctg ttcggcataa cacatcaaaa ttgaaagaat atattgcaga
     6721 agcacgtaaa aataaactca aactttcctc gccgtccatt aatcaaagta attccagttt
     6781 tgagttggtc aataaggaag aaatcagatt cggactgaca gcgataaaag gtattcggcg
     6841 tgattttgta gaagatatat taaacgaaag aaaacaaaat ggcccgttcc agtcagtgga
     6901 tcaattcctc atacggatcg ataagcgttg gctgaaatta gagttactgc aatcactgac
     6961 agcagtagga gtttttgatg aattatctcc taacaggaaa caattgatgc ttgatctcga
     7021 aggaaaaatc caaaatatcg tttacagtgg tggaagtctt gacttgctgg gaatcatggc
     7081 attaaaagaa gaagaagtcg ctgactatac actagaagaa cgcttgaagc tggaagagga
     7141 gtacttaggt atttatttgt caggtcatcc tgcagaaggg tttgatcgtc ttcgtttagc
     7201 taaacaagtt cagctgatta cagatatcgt ccctaatcaa ccagtcaatt tattagtgat
     7261 gatcaaggaa gttagagaaa ttcggacaaa aaaaggagaa ctgatggcct ttgttgaagg
     7321 aaccgacagt agtggagaaa tttctgtcac agtttttcca actctttatc gtaaatatcg
     7381 taatttgttt gccgttgatc gagtgatctt tgttcaaggg cggagcgaaa tcagcaaata
     7441 taatcaggaa ctgcaactaa ttggagaaag ctttgctgat cctaaccagt tagaagccca
     7501 gtatccttca caaacttgct atttgcgtat tacagaagaa cgggaagatc ccgagataat
     7561 gaaacaatta caagtgattt ttcagaaaca cccaggatat ataccagtag ttatgtatca
     7621 cgaaaaaacg caacgaaaag tagttctgtc agatgcttat tgggtggatg acagcaagag
     7681 tataaagcgg caacttattt atctgcttgg cgaagataat gtggttttca aataacttac
     7741 ggtaaaaaat actacttgct ttcatttttt ttcatttttt tatccgtaaa tcaagacaag
     7801 tgagaaaaat aatgttagaa tgttagtcgt ggttaacgca aaaatggttg taaacctaag
     7861 tcatacttag gataaagtat tttaatgagg tgaatgttta ttatgaaacg catcggaatt
     7921 ttgaccagtg gcggagacgc acctggaatg aatgcagctg ttcgtgccgt tgttcgtaaa
     7981 ggtatttacg aaggactgga agtatacggg atcaattatg gttttgccgg tttagttgct
     8041 ggagatattc gtcgtcttga tgtggcagat gtaggtgaca agatccaacg tggtggtaca
     8101 ttcttgtatt ctgcacgtta tccagaattt gcaactgaag aaggtcaatt aaaaggaatc
     8161 gaacaattga aaaaattcgg aatcgaaggt ctagtggtta tcggaggaga cggctcttat
     8221 catggcgcaa tggctttgac aaaacgcggt ttcccagcag taggtattcc tggaacaatc
     8281 gataatgata ttcctgggac tgatttcaca atcggttttg atacagcaat caatacagta
     8341 ctagaatcaa ttgaccgtat tcgtgacaca gcgacttctc atgttcgtac attcgttatc
     8401 gaagtaatgg gacgcaatgc aggagacatc gctttatggt caggtgtggc tggcggagcg
     8461 gatgaaatca tcatcccaga acatgatttt gatatggctt cagttgctaa aaaaatccaa
     8521 gaaggccgcg atcgcgggaa gaaacattgc ttgatcatct tggctgaagg tgtaatgggc
     8581 ggaaatgaat tcgctgagaa actttcagaa tttggtgact atcatacacg tgtatctatc
     8641 ttgggacacg ttgtccgtgg tggagcacca agcgcacgcg accgcgtgtt ggcaagtaaa
     8701 ttcggtgctt acgctgtcga attgcttcaa gaaggaaaag gtggattatg tgtcggtatc
     8761 catgacaacg aaatcgttgc gtctgacatc attgatacac ttgaaaacaa caaacataaa
     8821 ccagacttat cactatatga tctaaataat tcactttcat tttaagagtg aaagaaacca
     8881 agctatttaa gctgtgtaca gctttaaata aacaaaattg aatgggagag ttttttaaat
     8941 gaaaaaaacc aaaatcgtta gtacattagg accagcaagt aactcagtag aaattatttc
     9001 tcaattgatt gaatctgggg caaatgtctt ccgctttaat ttttctcatg gtgaccatga
     9061 agaacaatta tcacgtatga ctatggtccg tgaagcagtt aaaaagacag gaaaagatgt
     9121 cggtattctt ttagatacaa aaggagctga aatccgtaca actgttcaag gcacaacaga
     9181 agctgatttt ggtcgtgcag gatacatcaa atttgaagta ggagatcaaa cacgcatctc
     9241 tatggatcct gaacatgtag gttcaaaaga aaaaatcgcg gttacttacc caggattatt
     9301 tgacgatgtt cacgttggcg gacacatttt atttgatgat ggtttgatcg atatgcaaat
     9361 cattgaaaaa gacgaagcaa accgtgaatt agtcgtagaa gttaaaaatg caggtatgct
     9421 aggatcacgt aaaggggtaa atgcaccagg cgtttctatt agtttgccag gtattactcc
     9481 aaaagatgct gacgatattc gttttggttt agacaacgat atcgacttta tcgctgcaag
     9541 ttttgtacgt aaagcacaag acgttctaga tatccgcgaa atcttagaag aaaaagacat
     9601 gactcatgtt caaatcttcc ctaaaatcga atctcaagaa ggtatcgaca acattgacga
     9661 aatcatcaaa gtttctgacg gtgttatgat tgcccgcgga gatatgggtg ttgaaatccc
     9721 agctgaacta gtaccaatgg ttcaaaaacg cattatcaaa aaatgtaatg ctgctggtat
     9781 cccagttatc actgctacac aaatgttaga atcaatgcaa gaaaacccac gtccaacacg
     9841 tgcggaagct tctgacgttg ctaacgctgt atttgatggc acagatgcaa caatgctatc
     9901 tggtgaatct gcaaacgggg actacccagt tgaagctgtt tcaacaatgg cacgtatcga
     9961 ccaagaagct gaaaatgcat tatcagaatt aggtacattc caattgaacg aattcgataa
    10021 aacagatgta actgaaacaa tcggtttatc tgtagctcgt gctgcaaaaa atctaggcgt
    10081 gaaaacaatc gttgctgcaa ctgaatcagg atacactgca aaaatgattt caaaatatcg
    10141 tccagatgca gatatcttgg ctgttacttt tgacgaacgc acaaaacgtg gtttgatgtt
    10201 aaactggggc gtttacccaa cagttgctga aaaaccatca acaactgatg aaatgtttga
    10261 actagcagct aaaaaagctg ttgaattagg tttcgcatca gaaggcgatt taatcttgat
    10321 cactgctggt gtaccagttg gtgaacgcgg aacaactaac gtgatgaaaa ttcaaatgat
    10381 tggttctaaa ttattagaag ctcaaggcgt tggacaaaaa tctgtcgttg caaatgcagt
    10441 tattgcttca tctgctgaag aagctgtaaa aaaagcaaaa gacggaatgg ttctagttgt
    10501 accatcaact gacaaagaat atatgcctgc aatcgaaaaa gcagctgctg ttatcgttga
    10561 agaaggcgga ttgacttctc acgctgcagt tgtaggaatt gcacaagata ttcctgtaat
    10621 cgttggtgct aaaggcgcaa caacaacaat tacagaaggt gaattgatta ccgttgattc
    10681 acgtcgcggc atcgtttacc gtggcgaaac aacagctatc taatttagct taataaaaaa
    10741 taaaaacagc tcttctttta cgaagagctg tttttttgtg ctgtaacgaa ttcatggata
    10801 acttggacta cttgttcttg aagaggcgtg acgatctgct gtgttcgttt tgccaatgaa
    10861 atcaagaagc gaggctgaac ttcatctgaa agagggaggc aaactaaatg atcttcagga
    10921 tggacagcaa tctctgccaa aaaaccgata cccacatttt ctcggaccat actctttaaa
    10981 atcgtcaagt cattgctttg ataaacaact tttggatgag tatgtgtttg tttagaaagc
    11041 ttagtgaatg ctgcggggtg gacataatgt tcgttcaata gtacaaactg ttcatctgct
    11101 atatctttga aagagacagt atcctgcttg gaaagaggat gtgtaggtgg cacgacgatc
    11161 atgaaacgtt tgtccaaaag taactctgtt tctaaatatt gatccacgat tggctgtgtt
    11221 gagcctaata cagctaaatc gatcctccct tgtcggatca tcccgtataa gtcccgagag
    11281 cctccatcgg taatctcaat ttgattcatc aattcatgct tgataaagta acttgacagt
    11341 ttaggaaagt attcattgcc gataatagga ggcatgccaa aacgaagctt tttcttttgc
    11401 aactgagtga tttcagctct tgcagtagaa atctcttgga gaatggtttg tgcatggaga
    11461 aacagctgct ttccagaagg cgtgacgatt aattcgtgat gcgatcgatc tcttgtaata
    11521 agtacggtgt caaactcttt ttcaagacgt tgtaatgcat atgtaatagt aggttggctc
    11581 acacggaaat aattagccac tttcgtaaaa tttttttgtt tgacgatttg gacatagtat
    11641 tcgatatctt tcgtgttcat gaagatcatc cgctccttta atggaataat gattattgta
    11701 aatgattctg aggatgatag caaacagagg ctttctaata aagatttttc tgttttttta
    11761 tgaactgcaa ggtgtcaaga aaatttcatt gacaacatgc tttttgccct gtataattgc
    11821 ttttgttgct taggagtgat gaaaaatgaa atggtcttta ttggaattga gaaaatacca
    11881 ggagacaccg ttaacttttc atgagacgct cgacttgaaa gccgctctca tgaagcgaga
    11941 caatttaata cttgatgttg cgccagtgga agtcgaaggt ttggtctcag ttgataagaa
    12001 aggctatatc gttcattaca ctgtgcagac gactttgaca gttccttcaa cacgttcact
    12061 agaacctgtt gaactgccca tggatttttc tgtagacgaa gtattcatga cagaagaaca
    12121 attccaaagt agagatgaac aaatgtctgc ggatgagatt ttgctaattg aaggtccatt
    12181 attgaattta gcagagtcga ttgaagacaa catcttattg gcgattccaa tgcgggtatt
    12241 gacaaaagaa gaggaagcag caacagaact tccaaaaggc aatgattggg aagttgtttc
    12301 cgaagaagaa taccagcgca gaaaaatgga agaagccgag aataaaattg atcctcgtct
    12361 tgcaaaacta tctgaacttt ttgattctaa atcaaaagac gacgataaca agtaagattg
    12421 gaatatattg tgtctaattt agatacaaga ctttacaagg aggtgtagca aaatggcagt
    12481 accagctaga agaacatcaa aagctaaaaa agcaaaacgt cgtacacact acaaattgac
    12541 aatcaaaggg ttaaacgaat gtccaaactg tggtgaaatg aaaaaaagcc atcacgtatg
    12601 tgcaaattgt ggttactatg atggtaagga cgtaatgtca aaagaagctt aatacatttt
    12661 aattagaaaa tgtaaaacct caaggctaac catggagcct tgaggtttat ttttttgtag
    12721 tcggtaaata aaaaatatgt tgttctttag tttgcaatgt agccgtgtta agctataatg
    12781 taagagatta gggagaaatc ccgtatgtag gaggagttca tatgtcaaaa caacaaatcg
    12841 gcgttgtcgg tatggccgtt atgggtaaaa atctagcctt gaacatagaa agccgcggct
    12901 attcagtggc tttattcaat cgtacagggg ctaaaacaac cgctgtagta gaagaacacc
    12961 cagataaaaa tttcaaagca acatatacaa tcgaagaatt tgtagaatct atcgagaaac
    13021 cacgtcggat cttgctaatg gtcaaagcag gaccggcaac agatgcaacg atccaagagt
    13081 tgcttcctca ccttgataaa ggcgatattt tgattgacgg aggaaataca ttctttaaag
    13141 atacgatgcg tcgtaatgaa gagttagcca actctggcat caactttatc ggtacaggcg
    13201 tttccggtgg tgaagaagga gctttaaaag gaccttcaat catgcctggt ggacaaaaag
    13261 aagcctatgc gctagttgca ccaatcttag agcaaatttc tgcaaaagca gaagacgaag
    13321 caccatgtgt cacatatatt ggcccaaatg gagccggaca ttatgtgaaa atggtacaca
    13381 acggtattga gtatggagat atgcaactga ttgctgaatc ttatgactta atgaagaata
    13441 ttttaaacct ttcagtagaa gaaatggctg atatctttaa agaatggaat caaggcgaat
    13501 tagacagcta tttgatagaa atcactgctg acattctgac acgtaaagac gatgaaggaa
    13561 caggtaaacc agtagtagat gtgatccttg atgcagctgg gaataaagga acaggtaaat
    13621 ggacaagcca aagcgcgctt gacttaggtg tgccacttcc attgatcact gaatctgtat
    13681 ttgcccgcta tatttctgct tataaagaag aacgcgtaca agcaagcaag attttatctc
    13741 gtacgaacga ttttgagttc acgggagaca aaaaagaatt ggtcgaaaaa atccgagaag
    13801 cgttgtattt cagtaaaatc atgagttatg cccaaggatt tgctcaatta cgtgtcgctt
    13861 caaaagaatt tgattgggac ttgccatttg gtgaaatcgc aaaaatctgg cgcgctggat
    13921 gtatcatccg agctcgattc ttgcaaaaaa ttacggatgc ctatgacaaa aatcctgaga
    13981 tcgaaaattt attgctagat gattacttcg ttgaaatcac gaaaaaatat cagcaatcag
    14041 tcagagacgt cgttgccttg gctgtccaag ctggcgtacc tgtaccgact ttctcatcag
    14101 ccatcgctta cttcgattca tacagagcag aacgtctgcc tgcaaatata atccaagccc
    14161 aacgcgatta ctttggcgca catacctatg aacgtgtcga caaagaagga atcttccatt
    14221 actcatggta ccatgaagaa taatagtctt tcggctgtta ggaaatgaga aaataaaaag
    14281 aggacgaaag agctgaagtc gattgttatg agtagacttc agctcttttt taaaattgct
    14341 atccatgata aaatgactat agatttttaa gcatattatc ggtataatga tagagttttt
    14401 aaaaagccag tagaatagaa aggataaaat aataaatgag caatattcta atcattgaag
    14461 atgaaaagaa cttggcacga tttgtcgagc tagagttgaa gcatgaaggg tataacgcag
    14521 aagtccacta caacggccga actggtttag atgcggcatt aaataatgag tgggatgcca
    14581 tattgttgga tctgatgctc ccagaattga acggtttaga agtctgcaga cgtgtacgcc
    14641 aagtcaaaaa tacaccaatc attatgatga ctgcaagaga ttctgtgatc gatcgtgtat
    14701 ctggacttga ccacggagca gatgactaca tcgtcaaacc atttgctatc gaagaattat
    14761 tagcacgtct tcgtgcattg ttgcgtcgaa tcgatattga aggcgataaa aatgtggcta
    14821 aacaaactac tattacttat cgcgatttga ctatcgaaaa agaaaaccgt gtcgtacgtc
    14881 gcggaaatga agtaattgaa ctaacaaaaa gagaatacga gttgctgctt actttaatgg
    14941 aaaacgtgaa cgtggtatta gcacgtgacg ttttactaaa taaagtgtgg ggatacgaaa
    15001 cagaagttga aacaaacgta gtggacgtat atattcgtta tcttcgcaac aaaattgacg
    15061 ttcccgggga ggaaagctat atccaaactg tacgcggaac aggttatgtg atgcgctcgt
    15121 gataaagccg aaaaaatcgg aaaaaagaga actgaaaggg ccctccttga caatcaagtg
    15181 ggcctttgcg agttctttct tcatattcgt tgtgtttaca attttcgctg tcattaccta
    15241 taaatcatct atcaatctga ttgtggcaaa agaaagaaac aatgtagagc gtacgatttc
    15301 agaagtagct tctcgtttgg ctaactcgga tgaagaactg acattagtaa atacgtatcg
    15361 taatctgacg aaagcgacaa atgacggaga tgaggtcgta gatcatactt cactggtcga
    15421 aggaaactta atgaagatcg attcctttat agctgagcta gggcagccat caatggatct
    15481 ttatgtttat gatttggacg agaacttgat ttttaaaacc catgaaaaca gtcgaaacct
    15541 gattcagact acacgtaaag ttccaacgat cgttacacta gaaggaaaaa ctggcttttt
    15601 atctatccag cctatatatt ctaaaggtac aagagaaaag atcggctatg cgcaaagctt
    15661 ctatgaatta tcttctttct atgatattcg aaataagcta ttattgactc ttattgtttt
    15721 agaagttgtt tctttgatct taagtagtat tttaggtttc tttttatctt cttatttctt
    15781 aaaaccactc aaggtactca gagatacaat ggatactata cgtaaagatc cgcaatcaga
    15841 tatccacatg cctgaaatcg acacaaatga tgaattggca gatttagctg aaatattcaa
    15901 tgagatgctt gaccgtatgc gtttgtatat tgaacaacaa gagcaattcg tggaagacgt
    15961 atctcatgag ttaaggacac ctgttgcaat tattgaaggg catcttagtc tgctaaatcg
    16021 ttgggggaaa gatgatcctg agatattaga agaatcatta agcgccagca tgcaagaaat
    16081 tagccgtatg aaaagcttag ttcaagagat gctagactta tcaagagcag aacaagtaga
    16141 tgttcattat gcgaatgaaa caactaaagg aaaagaagtc acctatcaag tttataataa
    16201 tttcaagata ttatatccag actatgtgat cactttagat gatgatttga caaaagaagt
    16261 gaccctgcaa atttatcgta atcactttga acaattaatc attatcatat tggataacgc
    16321 agtaaagtat tcaactacac gaaaagaagt ccatatctct atttcttcca ctctgaatga
    16381 atttgagatc gcgattcagg actttggcga aggaataccc aaagaagacc ttaataagat
    16441 cttcaatcgt ttttatcgag tggataaagc aagagctaga acgaagggtg gaaatggtct
    16501 ggggttgtct atcgctaaac aactggttga aaactataaa ggacgtatct tagcagaaag
    16561 tgtagtaggg caagggacaa tttttagaat ctttattcct atcgcaaagg cagaagacaa
    16621 gtaaagcatg tgctttactt gtttttttat atatccaaca aagaataagg tacaaaatac
    16681 aagcaagtta gaaattcagg gatgtcatta taaaaaaaac tagcgaacaa ttaaatagaa
    16741 taacatccgt taattaaacg taagtagctt ttagatgaac gaaaaa
//
LOCUS       DAZKKW010000059        16457 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.060, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000059 DAZKKW010000000
VERSION     DAZKKW010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16457
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.060"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(114..1424)
                     /locus_tag="ACG8PO_001858"
     CDS             complement(114..1424)
                     /locus_tag="ACG8PO_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004650 - polygalacturonase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28 protein"
                     /protein_id="HFQ0207166.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     INFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1436..2854)
                     /locus_tag="ACG8PO_001859"
     CDS             complement(1436..2854)
                     /locus_tag="ACG8PO_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0207167.1"
                     /translation="MLSQNAVTANTVKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
     gene            complement(3094..4662)
                     /locus_tag="ACG8PO_001860"
     CDS             complement(3094..4662)
                     /locus_tag="ACG8PO_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HFQ0207168.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(4666..4947)
                     /locus_tag="ACG8PO_001861"
     CDS             complement(4666..4947)
                     /locus_tag="ACG8PO_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HFQ0207169.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAAGAFSLYRVRMRKGA"
     gene            complement(4944..5867)
                     /locus_tag="ACG8PO_001862"
     CDS             complement(4944..5867)
                     /locus_tag="ACG8PO_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectinesterase family protein"
                     /protein_id="HFQ0207170.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(5864..6742)
                     /locus_tag="ACG8PO_001863"
     CDS             complement(5864..6742)
                     /locus_tag="ACG8PO_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HFQ0207171.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPTVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIETLLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(6729..8066)
                     /locus_tag="ACG8PO_001864"
     CDS             complement(6729..8066)
                     /locus_tag="ACG8PO_001864"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HFQ0207172.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTSAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(8069..9172)
                     /locus_tag="ACG8PO_001865"
     CDS             complement(8069..9172)
                     /locus_tag="ACG8PO_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFQ0207173.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(9246..10754)
                     /locus_tag="ACG8PO_001866"
     CDS             complement(9246..10754)
                     /locus_tag="ACG8PO_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFQ0207174.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(10720..11412)
                     /locus_tag="ACG8PO_001867"
     CDS             complement(10720..11412)
                     /locus_tag="ACG8PO_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HFQ0207175.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(11434..12408)
                     /locus_tag="ACG8PO_001868"
     CDS             complement(11434..12408)
                     /locus_tag="ACG8PO_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0207176.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            12708..13580
                     /locus_tag="ACG8PO_001869"
     CDS             12708..13580
                     /locus_tag="ACG8PO_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0207177.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(14162..16360)
                     /locus_tag="ACG8PO_001870"
     CDS             complement(14162..16360)
                     /locus_tag="ACG8PO_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0207178.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEVEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLFSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
BASE COUNT         4842 a         3461 c         2672 g         5482 t
ORIGIN      
        1 tttgagctat gtacaagaag cattttattc tagcttaatt tgacaaatca tatatcatta
       61 aaaaaaataa aggtcaagct aaaatattat ttaagcttga ccttattata atttcaacga
      121 ttgtcaaaag tattgtttgc agctgtgttg ccagtttcta tagataaatc acagcttttg
      181 aaacataagt tttgtactgc ttcgattcgc ccgaattctt ttgcagttat ttggcaatga
      241 gaaaagcaga catcttctat aggcttttca ggaaatgcta ctaaatcaaa agctcgggaa
      301 ggaagtgagt aacctggaga aaggcatgcc tgtacgtttt tgatgtgtat gtctttgact
      361 ttcgtcattt gctttgattc aggtatttct tcagccactg cttgccaact ttctggcatt
      421 tgagcaaggt cattgctatt ttttctattg tattcattat gccagtccat tatccaagaa
      481 aaaggaaatt gtacatcaat catattcaga ttttctaccc tgatattttc gatcactccc
      541 cctctttctt ttgaagattt cattcgaata ccacagtctg actgaaaaaa attgatatcg
      601 tgaatatata cgtcactaac tccagcacta acttcgctac caatagttac accgtatcct
      661 gaacggattt tacatctagc aacctcgatt cttgatgatt ttcgattgac tcttgcacca
      721 ttgccatctc tgccagactt aatagcaata caatcatcac cacaggccaa atcacattca
      781 tagacacgca catttgttga agaatcaata tcaatcccat ctgtacttgg tccatcattg
      841 ttgcggatga ttactttttc tactaataca tcgttagaat aagtgatttg caagttccaa
      901 aaacctgatt tctgaaaaat caaatcacga acaacaacat gttctgcatg atacagtaaa
      961 caagcgcgtg gacgtttaat agcataatcc gcaatccaac gaagtccctt ttgatcgtat
     1021 tcagctcttg tcccgctttt ttgatctttt ccccaataca gctcccacca atggtctcct
     1081 tgaccgtcga ttattccttt tccttctata agaatatctt tcgccgagag aacattcaaa
     1141 atcgctgctg gccatttcat ttctacgccg gctactcgtg tatcgatttc tggaaatgct
     1201 tcgatatctg ttctcccctt gaggactgca ccttcttcaa agataagatg actcttatct
     1261 tttaaaaaaa gggctccgac taaataaatg ccttttggaa taaatacagg taagtgttct
     1321 ttcgcacctt gatcgattgc tttttgcagt attattgtat catcttgttg agtgtcgttt
     1381 gttttaaagt tcaaagcatt gataatccct ttttttgtcc gcaatacgtt tctccctatc
     1441 tttcattgat ttttttcaat tcttcaccaa taaatacagt ttttgaacga ttcagtgggt
     1501 aacctactag caaaatgatt gcggatacaa ataagcagaa tgctggtaac aagtttgcca
     1561 tcgtataaat cctttcttgt actaaaactg attggatggc tccacctgtt gtagacgatt
     1621 ggtaaccaat gagtgccagc ataaatccgc ctaccgctcc tgcacatgct tgtcctactt
     1681 ttctcgcaaa agaatttact ccatatactg ttccatcttc tcttaagcct gtcttatatt
     1741 gatgataatc aatcacgtca gtaatgaatg cccagaccat caaattgaat aaaccagatc
     1801 ctaaagtagc gataaataaa agaatcagat atacccatgc atttgttata tgaaggaaat
     1861 acatcagcaa gtacatacat gaagaagcaa gtaacgcaaa tacgcttgct tcctttttac
     1921 caaataattt tgtcagcttt gtagcaaatg gtgctaaaac tactactgtt ccgtaagtaa
     1981 ataaaagagc aacagacatt gcttgtttat tatggaagta atcattgaat aaataagtca
     2041 tattcgtccc agctaaaatc tgattgatca caatgaaaat gtcaacaatg actaaaataa
     2101 tcagtgctcg attagctaac aaacctttta ccaactgaga agcagatact ttttcgtttt
     2161 tgacaactct tactctttca gtagtcaatt tacaagttaa catatatgcg acaaatgcta
     2221 ttgcagcaca tccgattgaa atccaaaaga aatgattccc tgaaacgact tgttgtccac
     2281 tcttgttatg ggtatacatt aaaatcggaa tgaagaatcc tgtagttgat ccgccaatcg
     2341 cagaaccaat gcttcggaac gtggacaacg aggtgcggtc attaggattt gagctaattg
     2401 ctgctgccat tgctccatac gggatattga ttgttgagag aaaaatccca taaacaatgt
     2461 atgaaacaaa aatataagca atcttcaccg tcattggaag atgttggacg aatggcaaaa
     2521 acaaaatgac agtcactaag caaaatggat acttcatttt tctgatccaa ggaataaatc
     2581 ggccagtttc tgttaactct gagacatcac ataatcttcc aaccatcaca tcagcaaacg
     2641 catccaaaat tctcgaaatc aagaataacg tacctacgat tgcccctgaa acgccaagaa
     2701 cattcgtata ataaatcatc aagaaactat tgactaaacc taaaataaaa cagttaccta
     2761 gatcaccgaa catgtagcct atcttatcgg aaatcccaaa ttcctttttt tcttttttta
     2821 ctgtatttgc tgtaacagca ttttgtgaaa gcatttgaat tcctcctacc tcttctagta
     2881 aacgttttca aaattataac tctttttttc ttgtttttat attcacaatg ttggcttttt
     2941 attgcattat cttgacagat aaaaaacaaa aaataatcta aaaacattga tgcataagat
     3001 tgataacttt ttttacataa caatatatac aaacaaaaaa attcactaac tagtgaaaat
     3061 aagttagtga acctcattta ttaaagaatt aaattacaga tgatcttttc catcatactt
     3121 gaaaaactca aaatcagcat aacttcctct ttttttattt agatcatgca ctgctatccc
     3181 gataaaattt ccagtaaacc ctcctgagag tatacacatg tttttttgcg gacctacttt
     3241 cttccattct tcagttgata cgtctttcaa atgaaactgt gctgtctctt tctttacagt
     3301 gactttcaaa aaagtcttct tttctgtgag tggaatcgtc caatcttcta tttgtgctac
     3361 tccgttttct gattctatca tacgtaggca ttttcccttg tcctcattcc aagtaacata
     3421 acaataaata taattggatt catttaagta cagtaccagt ccagccatct gattaaaatt
     3481 attaggcatg tattcaaatg ctgtctccgc atcaaaagta aaatccgttt gccgaatagc
     3541 taacaaatga tgttcgaagg tagattgaag agattcaccg gaaatcagac gtaaaaaacc
     3601 tggtctacat tttgtatcac accaatcagc atttggcaaa atgcgtctgg cattccagtt
     3661 cttctttagc tctccttcaa atgtatccat aaactgatga tctttttctt gttgtacctc
     3721 ttcatttgtc tcaatcatta tttcttccat cggagttgtc tggctatctt taagtcttaa
     3781 ccaatcgtcg gacgtccaat acacttcttg aatagcggtt tctcttccca aaatagccgc
     3841 accgtttaat ggtctggtac aaagatatgt catgtaccat cttcctgacg gggtttgtac
     3901 taagctccca tgtcctgtac attgcaatgt agaagcaggt ttgtcactag ctgtcagcat
     3961 aggaaaacca ggatcaagct cgtaaggacc agtaatattc tttgctcggc aaactgtaac
     4021 tgaatgtcct cttcctgtac cgccttctgc cgtcatcaaa taataatacc cgttacgaaa
     4081 atacaaatgt ggtgcctccg ttttcgctaa ttctgtacct gcaaatattt tatagatggg
     4141 accatcaagc ttcttcttct ctgcatcata ttcttgtaat acgataccgc tggatttatt
     4201 cggtgtgtcc agacgataat cccatagttc attgacaagc catttttttc ccgtttcaag
     4261 atcatggaac aaagaaggat caaagccgtt actatttaaa taaattggtt cggaccaagg
     4321 acctgtgatc tcagatgcag tgatcaaata attgtggaca tctttaaacg gacggcctgc
     4381 acttttgaca tctgtgtaaa caagataaaa caatccatca gcataactga tctgtggcgc
     4441 ccatatactt ccatttgtag gatttccttg caaagatact tgagtggtca aaatatccgt
     4501 tgtatgtgac cagttgacta aatttttcga ctcatatact cggattcctg gaagccattc
     4561 aaaagaggag acaataatgt aataccgctc tttcactctt aatatattag gatctggatt
     4621 gaatcctctt aaaataggat ttttcacttt gatagtctgc atgattcagg ctccttttct
     4681 cattcttact cgatataaac tgaatgcccc agctgctaaa aaagcaagtg aaataaacaa
     4741 cacaacggca aacagaccag tcatgtcctg taataatcca aatgtatttt ctaaagacaa
     4801 agttgaatga tctacagcaa gcgactgata acttttttta aactctgacg cagacatatt
     4861 acttaaagta actgctaacc cgccaaagaa aatcaaagat agactgccac cgaccattcc
     4921 tagcaatagt tcccattttc ttttcatttc tgaaccccct gcttttctaa atgtatccat
     4981 tcaggtcttt tgatcgatgt ttcataattg ttattacgtt cagagaattt gacagtctgt
     5041 tcatttttct tgtcatccca attatcccaa ccagttgggt gaacatgccc acctatctta
     5101 cacgcttcaa ataagacatt tgcatacgga cgccacggtc ttcctaaata gacgtttcta
     5161 gtatcaggtt ctgcagtcaa ggtacagctt tcaaaacgat accctgtttt ttgatttttc
     5221 ggcgtagatg ctgctgtgat gtatccagga tttttactgg ctaagctatg gatatgacac
     5281 tgatgaaaaa cggcatctgc cccaccaaat atgaaatcca ccgttccttc aatccagcaa
     5341 tattcatagg tctgacgaca atactcagga ttggcagaag ccggtgtaca aaaaggagta
     5401 ccatcttttt gcaaatcagg caacggaccc gtgcataagg tatcctgata accagatagt
     5461 cgacagtttt ttaaagatac gcgatctcct gaattgaata aagcaacagc ctgtcctacc
     5521 ttttctcctg gacctgctgt attttcgatg tgaagatttt ctaatacgac attcgatcct
     5581 tctaagaaaa aagtagcact tcggaaagtc cctaactggc taccgtcaag atactcctgt
     5641 tttgcaaaac gattaccgga gataactact gttcctgctc ccacaagttt aaaattggat
     5701 aatctgcttt ctatatattc atcatatctt ccttctttta ctatcaagac ctttggatgg
     5761 ctatccggca ggcctgccaa ataatcgatt ccttcttgga tagtttcaaa atccgtcggg
     5821 gtatctatag cattctttcc aattatcagt ttgtctttca tcctcataaa tgctccctta
     5881 tgaattgttt ccaaatattt ctcatttctt gtgtttccat atgaccgcct gttaaagaaa
     5941 aagaagaaaa gttttctgga ctacccattg attgatactt tttgtccagc ccttttctca
     6001 gcttcagtac gccttcgtcc aagcacatcg tatcgttttt tccaactaag ctaagacgag
     6061 ggcgcggagc gattttttct tggattgcta acgttgtata agctgtcaat agatttggca
     6121 catagtaata aaacccatga tgatctaggc ggcgatgtag caaaagtgtt tcgatgtcca
     6181 cctgtgccgc tagatcaata caaactttga cacgagaatc caatgctgtc acccaccagc
     6241 ttaataagcc tcccatagaa agaccaatcg ttgcgagccg ttgagtatct gtgtcttccc
     6301 ttgtctccaa ataatcgatc aagctgatca catcataaat acgcattccc cataacgttt
     6361 ttcctgttaa taaaaattgt ttgaacaact cgctttcaga aatacctccg cgttcttcaa
     6421 atccccaagc atcaatcgac caaacagcat atcccattcc tgtcagtact tctgcaaaag
     6481 atggagaagc taagtaagga gcaccatgga tcaattcact tttcccgact gaaaaatctc
     6541 cgccgtgaga atgcaggtaa aggaccgtcg gaaacggtcc ttctttttct ggataagcat
     6601 agagggctgg tactgtttcc aattcattta agtacaattg atagtattca agcataaaac
     6661 cgttttgttt ttctcggtat aatcgttttc cactaggggt atgtctgcta gatggagcac
     6721 ctaaaaaatc attcaactgc ataagcaaag tttttacgaa caaccagttc atccatattt
     6781 tctgattgat ttgattggaa tttctcaata ctgatttctt tagtattttc taatagaaat
     6841 gctggtccct cgtgattttc aatcgacatc tggttaaagg aaaccttacc cgcaaagcca
     6901 atataaaatc cttgtctgct catttcctct attcctgtca tcatagcagg aacaccggga
     6961 actgcatctt gggccatcga gacatcgata ttgtcaaatg tgatttcttg tatgaattgt
     7021 tccgctaatc cataaataaa acctgcagaa gcatggacat tccttgccgt gatattcgaa
     7081 aaatggatcc ttctgaatgc tgatgtccgt tcatctatcg gataagcctt tttttcccat
     7141 acatacggtt ctttccctcg cggaccacag aaataataaa gattcagtat aaatggacac
     7201 atcacattat ccatgactat attggatact cgaatatctt caacgatccc gcctcgacct
     7261 cgtctagatt tcaatcgtat tcctcgatca gtttcttgga agatacaatt tgaaatggta
     7321 atattacgaa tactaccact catttcactt cctaatacga caccgccatg tccatggacc
     7381 atcgtgcaat ttgtgatcgt gatattttcg caagcgatcc tttcgtacgt atcttcagtc
     7441 cctgccttga ttgcaataca gtcatctccc acgtcaatat ggcaattact aatacgcaca
     7501 tttttgcatg attctggatc aattccgtct gtatttggtg aatccgctgg gttcaagatc
     7561 gtcaagttat caaacgttgc attactgcaa agaatcgggt tgatcgtcca acttggtgac
     7621 tggatcaatt taatatcttt cacagtaatt cgatgacagt tatggaaact catcaattta
     7681 ggacgaggat aagcgagatt atctggttca tttctaaaag tatgccacca tttttttcca
     7741 tttccatcta atgtaccgaa accagtgaca gagatgttct ctacattttg agcatagata
     7801 caggaagcat aaacttttcg atggactcct tcccaacgtg aatgaacaac aggataatct
     7861 tttggatcgt cagagaattt cagaaccgct cctgcgctta aatgaagttc gacattgctt
     7921 tttaaaaata aagctcctgt tagaaattca cctgctggaa caactacggt cccgcctccg
     7981 tcacttgctg cagcatcaat ggcttgttgt attgcttcag tattcaattc atcaattgat
     8041 gcgccaaatg tcaaaatatc atacatattt ataatgcctc cacttcaatc aatgcttgca
     8101 agaatgcacc aacgcctttt tgatcattac aaataattgg ttcactgatg tagtaagcat
     8161 atgaaccatc tcgctgatct gctccgccta accctgctac ttgacagttt ttattcaagt
     8221 tcagccagcc ttcttttgtt tccagaacaa attcatcaag taatccttga tgtgatttct
     8281 tcacttgtgg aagccattct tctttgttca gcagtcccaa acgtattcct ttagccattg
     8341 cgtaaacaaa catactactg ccggaagctt ccagataatt tcctttccgt tttgtttctg
     8401 ttgttacttg ataccatgtc ccgctgtttt cttcttgata tggaagtaat gcagccagtg
     8461 tcttttctag aatttctttc agcaaagaac aatctgtttc ttcatgaagt aattcatagg
     8521 tatctgctgc agccatcaaa taccagccaa tcgatcggct ccaaaaatga tgggatagac
     8581 ctgtttttgg atcagcccat ggttggacct tcttttcatc ccatgcatga atcagcaaat
     8641 ttgtttttgg atcgaataga tgtttataac tgatttcaaa ctgtctcaaa acatcttggt
     8701 aatctttttc ttcactaaaa ttctggataa attcagcata aaaaggagaa cccatatata
     8761 agccatccaa ccaaatttga taaggataaa tttctttatg ccaaaaagct ccttcggatg
     8821 tgcgtggatg gtttgccaac tgctgggaaa ggagttctgc tgctttttta tatttttctt
     8881 cgcctgtttc tttgtacaat aaaaagaaaa gctttcctgt atttacatgg tcgatattgt
     8941 attcttcaac gctgtagcca cggatcgttc catcttcttg cacgaaatag tcgatattgt
     9001 cttttatgaa atcaaaataa attttttctt gggttcgttc ccaaagtaat tggaaccctt
     9061 taagtacgac gccatagtca taagaccatt ttcctttata ccacttttct tcatataagc
     9121 gaggtgttct ttccatgata ctttttgcta atttctttga ccagttttcc attatttgct
     9181 ctcctttaaa aattaaaata ggatttggga caaataccaa gaagtatttg tcccaatcgt
     9241 ctatattact gtgccgctgc tttgtatgct tcctcgtact cttgtgtgat tcgatcgcct
     9301 ccttgtgctt tccagtcttc tacagctttt ttgaatccat cttcgtcaag ctgtcccatg
     9361 atgtattgca ccgtagcatc aatgacgatt ttttcaattt ctgatcctac ttcattgttc
     9421 gtcggtgaat ccaatggcac agaaggatcc attacagcaa attcttcatt ttctttgatc
     9481 aattgattag agaattcttt ttcagggtct gtatctttga aagagaacgt cacttcacta
     9541 ggacgagagc ttgataacgg ttgcacctct tgttgccaaa gatccatatc gataaattcg
     9601 atagaaccat catcattcaa ttcataatgc gttccttcga taccgccagt catcaattta
     9661 tacacatctt cttctaaaag atcatcaaca aattgaagta atttcttcaa ttgtgcttcg
     9721 tcttttactt gagatttggg aaatgctagc aagccgccaa taccatttcc ttctgaccaa
     9781 acatgataac cgccgccttc accatctgag atcttgttta caggcactaa ttccatatca
     9841 tcttgaatac cttgacccat cgtcttcaga ttcttgatat ccaccattcc agtgtagatt
     9901 ccagttcttc cttgtgcaaa ttgttcttgc tgatcagtct tttgggtaac tgcaaagtct
     9961 tgtgccaaat agccattctc aaatagatct cttgaaaaat tcaaagcttt catatactct
    10021 tctgtatcaa attctggagt gaatttgcca ttatcgtcta cagcccagcc atttggtgct
    10081 ccaaaataag aagtgaaaag tttaaagctg gtataacgaa tatcactagg cgcacgatcg
    10141 ccaaaaccaa ctgtgtcgtc cacaccattt ccatcaggat cgttttctgt aaattgttta
    10201 gctacttcat aaagctcatc catggtagtt ggaacatcta gtcctagatt atccaaccag
    10261 tcttttcgaa tgactaagcc agcacgagcg tagtcttttt gtatcggtac gccgtacaag
    10321 gcaccgtcga tactcgcagc ttcgattcta tcttcagaaa tttgacttaa atttttatag
    10381 tcttttaaat attctgatac atcccagaat acgcctgatt ttaacgcatt gcggacagaa
    10441 gagtttttca acatcgcaat ttgaagtgta acgatatctc ctagttcatc agaagccaat
    10501 gcagttgtta atctttcttc ttttgaagca tcaggaatcc agttgaatgt cagttccgta
    10561 tcggtttttt cttcgatcaa gttcacaatc gtgtctgtag gcggtgaagc atgatgcaaa
    10621 atatttaacc aagtgatttt agcttttcca tcttctgtct ctgaagaact gttacttgaa
    10681 ctgctgccgc atgcggcaag tgctagaatt cctgcaaaac taagacctaa ttttacgagc
    10741 gttgcttttt tcataaattt tctccttcac tgttttaata atatttttgt tttcttctgt
    10801 tttttctgta tatgagttct tccattccaa atggcaaaat atagaatttg ctgtccacgt
    10861 gactgcggaa aagaagagac tgccaccgat aaaggttaaa acacctggca ttgtttggat
    10921 gatcatcaga taaatcccta ccatacttaa tatcaacact aatgagtaat gaagatgtga
    10981 gaatccgaat aaaaatgcca ttttcatttt gaaaaaatat cctttccaat cataatgagc
    11041 catgattggg aaaatataga gtgctgagag cacatatatg aacgcagcaa tatacaaagc
    11101 gatcctaaga taccaattgt gtatgaaaga aagatccaca agtaaaacaa tcccgatggc
    11161 gccatatatc cagctcatca gcaaggtttc acgatagttt tctttaaaat atgtgaagta
    11221 cgttttcgtc aaaggaaacg tttcttttga gcgcagccat tttctaatca ctgcaaccat
    11281 cgcatacgat gcaggaccga aagtaaaaac tcccaaacct gcgatgacta aaccgcccca
    11341 cagaaaattc aaataaacca actgtaaaat acgatttagc cttccgttta ttttatctaa
    11401 tgactgcagc atatctttcc ctctctttct atcttagtaa acgccgtctt ctcctagctt
    11461 tttggcaatc ttattagcta aaattaccat aaacaatcct acgacccctt taaacaatcc
    11521 cattgctgta ctgaaactta actgtccatt cttcaatcca gctgtataaa tataggtgtc
    11581 gaaaatttct gcaacgcttc gattcaatga attcaataac aagtacatat gctcgaaccc
    11641 taagtctaat gtctgaccga tttttagaat cagcaaggta atgattactg gacgaattgc
    11701 cggcaatgta acatgccatg ttttgcgtaa gcgtcctgct ccatccattt ctgcagcttc
    11761 atacaactgt gtatcgactg cagttatcgc tgaaaggtag atgatcgtag accagcccaa
    11821 ctctttccag atcacttgtc cgatatatac tgtgcgtgtc cattcagggg atgttagaaa
    11881 actgattttt tcaaatccca ttgttgcaag gaattcattc aatacccctc cgtcaacatt
    11941 caaaaataca taagtaatcg aaacaatgat tacccaagac atgaagtgag ggatgtaaat
    12001 gatcgtttga atcccgtttt tcaatcgttt gttcgttact tcatttaata gtaaagacaa
    12061 aatgattggc atcggaaaaa acacgaagat atttaatccg aacaagatca acgtatttct
    12121 taataatgta aagaatgtcg gttctgtaaa caaacgaatg aaatgtttga aaccgaccca
    12181 aggacttccc attattccta aaaatggttg ataatcttga aaagcaataa cgagtccccc
    12241 cataggaaga tacctaaaaa tcaagaaata aaggactccc ggcagaatca tcacatataa
    12301 aaatttattc gttttgattc ctgcaattat tttttttcgt cttctgagct tttcttcttt
    12361 tttcgatagc gtcagttgaa atggttcttg tttctttaca ctttccataa gcccctcctt
    12421 agttttgtga atgtttgctg cttatgactt catgatagat aactatctga attacgtata
    12481 taatcatttt tatcgtaagt aaaaacgctt acatagcaat gatttttgtg ctatacaaaa
    12541 cgataaagtg tattttttca caaataaact acgcatagca aaccttctat ttttttctat
    12601 ctgacactta gcataggaaa tgattatata cgtaatcttt caccgatgat aaattgaaca
    12661 tatagaaaca gaaacattta atttattttg aggaggagcc tatctatatg aaaaagaaag
    12721 tctctatagg agaaagaagt tttacacttt tcaacaatac gtttctaatt ttgctagctt
    12781 tgatctgtat cgttcctttt ttaaatatca tcgctacatc ttttgcttct acacaagaag
    12841 tcgtcgcgaa aaagtttatc ctttttccta ctaccttttc tttagacgcg tatcgctaca
    12901 ttctttctac gccgaccatt tttagagcac ttgctgtttc gattggtgtt acaggtgtcg
    12961 gaacgatcgt cagtatgtgt gcaacttcac tgatggccta cggtctttcc agaaagtatt
    13021 tgtttggtcg aggtttcgtc aactttcttg ttgtattttc tatgttgttc agcgggggga
    13081 tgattccaac gttcttagtt gttcgttctc tagggctggt caattcttac tgggccatga
    13141 tcctgcctgt ggctgttaat gcaatgaata tgatcattat gagaaacttt ttccaagcct
    13201 tgccggatag tttggaagaa tcagcaaaaa tggatggttg cacagatttt ggtgtatttt
    13261 tcaaaattat gctgccatta gctttaccgt ctattgctac tatctcgctt ttttatgctg
    13321 ttacttactg gaacacttat atgactgcta ttttgtatat caacgattca tctaaatggc
    13381 cgatacagat tcttttacgc caaatcgtca ttgtatcaag tgggatgcaa gcagaaagta
    13441 gtgccgttga tgtgattcca ccagcacaaa cagttaaaat ggctgttatc gttattgcaa
    13501 cagtgcctat gctgattgtt tatccttttg ttcagaaata ttttgtcaaa ggagcactcg
    13561 taggttctgt caaagggtga ttatgtactg aacgcaaaat ggtagttgtg tcagaagtgg
    13621 tcagcttcaa gaaataagaa ggcatttacg aaaattgctt cttaaatttt tgtgaaattt
    13681 cggcttattt ttcgaagaat ctactcctgg aacaccgtgt atcagaggtt gtgaaagaga
    13741 cgttcagcct tgagcgatga taaaaactct cacaaacatt gcgacgagta acccacagga
    13801 gcaataagaa gcgattttga aaaatagctt cccatatttc acaaaattat gccttattgt
    13861 cgatagcctg tctcttgaac gccatttatt cgtaaaaaag aactgtgaca agacttttgt
    13921 cacagttctt tttgcgtgct tagccttgaa atttaactat ccataaatac aaatactagg
    13981 gttattctaa cttcttttat tgcttgaatc agtccacaag aaaaacagcc tcttgattaa
    14041 acggcgttta tcttgttgat tctgcggcaa taaaatcagc cagcagcaaa acatgaagtg
    14101 ctgttttttg ctgtcctctt ttattgcttg aatcagatca caagaaaaac agcctcttga
    14161 ttcacaaata tttttttcga tattctcccg gcgtaatcga ttccttcttt ttaaagaaac
    14221 gaataaaatt ttgcggattt cgatattgca atctttctgc aatctctttt acggagaggt
    14281 tagtctcttt caaccatttt tttgctacct ctaaacgatg attttgaaca aaatcaccaa
    14341 atttttctcc tgtttccttt ttaaatacat tacttaaata gttagggtta taatgcaacc
    14401 tatcagcgat cacatctaaa gacagatcac gatcatattc tgtgtagacg atatgcataa
    14461 tattctccga caaattttta aattcatgat cagtgattgc ttgagagcat tctgtgattg
    14521 gcagaactaa ttgttctaaa acaagttttt ctagttcttt gggatgataa ttgttcagtg
    14581 cttttacata aatttgtttc atgttatcaa agttttttga atctactccc aataactgtc
    14641 ctaattgaac gagttcattg atcaagcgga ccgaaactac ttcccgactc aaaggatttt
    14701 tattttgtgc aaacaatgca tcaatcaatt catgtactaa attcttcact tcctgatttc
    14761 cagaacgaat ggcttcaaac aatcgatttt gttcttctat cggatatcta ataagtgttt
    14821 tttcatgagc agcaggaacg atttcctgat aaaaaataat tgaacttgga ccagtattta
    14881 cacgataata cagggcttct tttgcccgat caaccgattg cttgctttca ttcagcgttt
    14941 cgaaccggtt actgatgcct atactgatag ttaaattcaa gtattctttt acacgttttt
    15001 gaatgagtcg gcaatactcc atgacttttt tgcttgcttc tgcatcatct ttatccattc
    15061 gataaatagt ggcttgcatt tcttcattca aaacgatcgg cagcattcga ttttcctttg
    15121 gcacaatttc cgtgatcatg ttgttgatcg ctaaaagtag caaatcacgc tccccaccgt
    15181 gactatcttc taacagatcg atttgaacca aagctgtata atagcagtca ttttgagaac
    15241 tataaccaaa ttgctgcaac cgctgatcta gttctcgcct ttctactcga tttctaaata
    15301 aactaagtac aaatagcgtt tccagctgtg gcttttgggt aactaagtgt gcacttaacg
    15361 cttctttttc tcctatgatc cgactgactg attgagcgac caaatctagt tctttcccac
    15421 tgaatccttt tgatttaaga ttcagacgtt cctgtatttg agaaatcgga cgggcaaatc
    15481 tgtccgaaaa taagtaggaa aaaagaccaa tgagtcctac cagtgttaaa gacactgcaa
    15541 ctagcccgat tctcagattg cggatcattg cgccaattgc actagagtca acttctaatc
    15601 tgtaaatcca attattataa tctgatttca gtactatata cgttttatct ttttctctta
    15661 tcactttcag ctgattgtaa tctagatcca cgtccgttaa tacagttggt tgctctttat
    15721 tttttactgt ctgagtagta aaaaggttgc caacttcgtt ttcaatagaa agtatgtctt
    15781 ctccactttc accaacaact tgttctattg tatgtttctc tatcgtagca attcccaatg
    15841 catatttttc tttttcaaac attggcaaag aaatcaccat ttcgattcct ttgtttttct
    15901 tttgccaaaa taaattattt ttgttattta tgtacttctg ttgatacttt tcaacttcct
    15961 cttcggtcag ttgactcaac gatccattga ttactcccca tttaccagcc aaactgacta
    16021 aatcataatt attatcatcc ataacaatcg tttctataaa atttagttct ttcctgattt
    16081 cagaataagt ggagtaatct gtatatgtca gcggatgctt gaatatgtcc gagaaatttt
    16141 tggtcgaacc atacgtagaa aacgaatatt caatcgttct aattttccat tcaacgttag
    16201 ttttaacttg cctgagcaaa ttctttttgt ctgttaaata actcgattcg atcgattcat
    16261 aggtattctg gtaaatgaat aaactaaatc ctatgacaag tacggctcct aataaaaaca
    16321 tcggtaaaaa tattttataa aatactgtac ttttcttcat tttcttcctc tcctcctttt
    16381 aaaataaaag gctttcattt ttctgtaaaa ttgatactca tctaattaaa tgagcattcc
    16441 ccgctttcaa gaatagc
//
LOCUS       DAZKKW010000060        15746 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.061, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000060 DAZKKW010000000
VERSION     DAZKKW010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15746
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.061"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(27..725)
                     /locus_tag="ACG8PO_001871"
     CDS             complement(27..725)
                     /locus_tag="ACG8PO_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HFQ0207179.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(718..2070)
                     /locus_tag="ACG8PO_001872"
     CDS             complement(718..2070)
                     /locus_tag="ACG8PO_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurT ligase domain-containing protein"
                     /protein_id="HFQ0207180.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(2293..3693)
                     /locus_tag="ACG8PO_001873"
     CDS             complement(2293..3693)
                     /locus_tag="ACG8PO_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFQ0207181.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            3854..4591
                     /locus_tag="ACG8PO_001874"
     CDS             3854..4591
                     /locus_tag="ACG8PO_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HFQ0207182.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            4716..5195
                     /locus_tag="ACG8PO_001875"
     CDS             4716..5195
                     /locus_tag="ACG8PO_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HFQ0207183.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            5192..5959
                     /locus_tag="ACG8PO_001876"
     CDS             5192..5959
                     /locus_tag="ACG8PO_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6681 family protein"
                     /protein_id="HFQ0207184.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            6189..6758
                     /gene="lepB"
                     /locus_tag="ACG8PO_001877"
     CDS             6189..6758
                     /gene="lepB"
                     /locus_tag="ACG8PO_001877"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFQ0207185.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(6862..7464)
                     /locus_tag="ACG8PO_001878"
     CDS             complement(6862..7464)
                     /locus_tag="ACG8PO_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFQ0207186.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            7570..7800
                     /locus_tag="ACG8PO_001879"
     CDS             7570..7800
                     /locus_tag="ACG8PO_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0207187.1"
                     /translation="MPISFVKDREEKGKCVREILLDLPEWFGLPESTEKYIEESSKLP
                     LWCEKRKEEYLGFITLSQTSEDTAEIYSIVWE"
     gene            7791..8039
                     /locus_tag="ACG8PO_001880"
     CDS             7791..8039
                     /locus_tag="ACG8PO_001880"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0207188.1"
                     /translation="MGIKKDYHHQGLGTKLFKEAEGYAAKHYKYLQVKTVDEGHYSIY
                     DQTICFYESLGFSRLEVFPNLWDEWNPCLVLVKKLEQK"
     gene            8145..8729
                     /locus_tag="ACG8PO_001881"
     CDS             8145..8729
                     /locus_tag="ACG8PO_001881"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFQ0207189.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            8732..9805
                     /gene="prfA"
                     /locus_tag="ACG8PO_001882"
     CDS             8732..9805
                     /gene="prfA"
                     /locus_tag="ACG8PO_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFQ0207190.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            9798..10637
                     /gene="prmC"
                     /locus_tag="ACG8PO_001883"
     CDS             9798..10637
                     /gene="prmC"
                     /locus_tag="ACG8PO_001883"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFQ0207191.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            10630..10809
                     /locus_tag="ACG8PO_001884"
     CDS             10630..10809
                     /locus_tag="ACG8PO_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0207192.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            10831..11856
                     /locus_tag="ACG8PO_001885"
     CDS             10831..11856
                     /locus_tag="ACG8PO_001885"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFQ0207193.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            12031..13275
                     /gene="glyA"
                     /locus_tag="ACG8PO_001886"
     CDS             12031..13275
                     /gene="glyA"
                     /locus_tag="ACG8PO_001886"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFQ0207194.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            13428..14375
                     /locus_tag="ACG8PO_001887"
     CDS             13428..14375
                     /locus_tag="ACG8PO_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HFQ0207195.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            14509..15138
                     /gene="upp"
                     /locus_tag="ACG8PO_001888"
     CDS             14509..15138
                     /gene="upp"
                     /locus_tag="ACG8PO_001888"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFQ0207196.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(15277..15519)
                     /locus_tag="ACG8PO_001889"
     CDS             complement(15277..15519)
                     /locus_tag="ACG8PO_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0207197.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            15640..15712
                     /locus_tag="ACG8PO_001890"
     tRNA            15640..15712
                     /locus_tag="ACG8PO_001890"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15673..15675,aa:Thr,seq:cgt)
BASE COUNT         5350 a         2823 c         3196 g         4377 t
ORIGIN      
        1 ttgtcacatt cccctttttc gaataactag gctgtttgtc cttcaagata gggcagatcc
       61 aattctttac cgtaacgttg ttctaaagct aggcggatca aacgagctgc caggttctca
      121 tttcttgcaa ggataggtcc atggaaataa gaaccgaaga catttcggta aatgacacct
      181 tcagattggt cttcaccatt atttccttga ccttgtacca ctttccccaa aggtcgttca
      241 ccttcaccta agaaagtccg accattgtgg ttttcaaatc cataataagt ttcattgaat
      301 tcttcgttat ggataacgat atcaccgata aaccggttat tttcctgact taacgtgtag
      361 tgatccagtg cactgattcc ttggatcttt tctccattag ctcccatgta gtagtgacct
      421 agcaattggt agccaccaca aatggctaac atgacgccat cattttcgat atagttgatc
      481 aaagaatctt tctttttctg gatgtctttt gaaatgatta attgctcaaa gtcttgacca
      541 ccgccaaaga aaaccaagtc atatttatca gcatcaaaag gttcatgtat actaacgatc
      601 tccgtatggc aagaaacccc cattttttct gctatatatt tcaacatcaa aaggtttccg
      661 ttatcaccat atgtgttcat caaatttcca taaaggtggg cgatattgag ttcataatta
      721 gccacggttc attcctcctt tgataaatcc ttgagcagct agttctttac gaagctgcaa
      781 tacagcagtg taagtagcta aaatatagac atgctcagtt ggcaattctt tgattttttc
      841 gattacttct ggtaattctt ttgtttctgt caatttattt tctggaatcc cggcaacttt
      901 tagacgtaaa gtcatatctg tatgacggtc tcctccagaa ataacagctg ggatatccat
      961 atcggcaaag ttttcaaaat taccatccca aatccagctg acatcgattc cgtctgcgta
     1021 attggcattt aataaagcca ctaaagaaaa tggataagga gctagaccaa tcatatcgat
     1081 tacttgattg atacctacag gatttttgac aagtactaat gtacattttt tcccattgat
     1141 attgatgact tcttgtcggc cgaatacttt ctcgtcatat cctaaacctt gtttgatttt
     1201 atcaggatcg acattgaagt actctgccac tgcagtagct gctaaagcat tgtagacatt
     1261 atacataccg ccaacttcga ttccgtattc atgaccatcg atcacgaaat ctgctgacac
     1321 attgtccatc cgtaccatat cagttaatcg ataatccaat tcaggacgtt tgaagtcaca
     1381 atttggacag taatatttcc ctaagtttgc ataagtgatc attttataat gcaagatgtg
     1441 atgacaattt ggacataaga caccgtctgt attgtaatga gccatttgtt cacgatcttc
     1501 ttcatggtca aagccataat attttctagg attgactgtt tcgatagaat tgaagattgg
     1561 tgaatcaccg ttactgataa tcgttgcatt tggtgacttt gcggcaccgt ctacaatcat
     1621 tttataggtc gtatagattt ctccataccg atccatttga tcacggaaaa tatttgtaaa
     1681 taagaacagt tctggtttca tatattctgt gactttgctc aaacttgctt catctatttc
     1741 taggacagca aactttttct gtcctttttt tggttttgcc tgcaagaaag tcgatacaat
     1801 tccctgtacc atattcgcac cagtagggtt ggttagaact tcatcgaatt gctggcgcaa
     1861 aatattgact gttaaagcgg tagtcaatgt tttaccgttt gttccggtga caacgacgac
     1921 ttggtagtct tttgccaaac tatctagtat atgtggatcg actttcagcg ctagtttacc
     1981 tggtaaactt gatccgcctt taaaaaaagt ttttaatgcc cattgcgacg tttttccaac
     2041 tgcaatcgca aaatgactgc gtattcccat tatgtaacct ccaaaaacga tttgctgacg
     2101 aataaattca actaatcata ccatatgttg aaaaaaaggt taaattgctt ttcttattct
     2161 taagaaaagg ttacggctga aagtgcatga aatcggacaa aatcgcatga tttaacaaaa
     2221 aaacagcttg aaacaaaatg attgtttcaa gctgctgtga ttttattatt ccctcttacc
     2281 tatattccca ttttaaccga taataatttt ctcgcttgga tattcatagc ctagatctcg
     2341 tgtttctttt ggcacgaata acatcaatgt atatagcatt ccgatccgcc caataaacat
     2401 cagtgtagca atcgtgattt tgcccacaac agacaagtct ccggtaatcc ctaaagataa
     2461 accagttgtt ccaaacgcag aagttacttc gatgatgatc gagatcagcg tttgttcttc
     2521 tgtagcagat aaaaaaacga tacaaaagaa acacatacca agagaaagca taaagacaac
     2581 gactgatttt cgcacatcat cttgatcgat tctccgtcca aaaatattga tgttatcttc
     2641 acttttgagg aaggaatata gatacaaacc gatgattgct acagtagttg ttcgaatccc
     2701 gccgcctaca gaactaggac tgcatccgat aaacatcagt aaagaaaaaa tgatcagtgt
     2761 tgtgatttga aaatcaccca aatcgtgaat ctgcagacca gcattacgag tcgtaatcga
     2821 ataaaacatc gaattgatcc atttgacact catattggaa tcttggaata aatggtcctt
     2881 ttccaataga taaatcaaga cagttcccga aacaaacaag ataacaaaag ctaacacagc
     2941 taatttggta aacaaggaaa aacgaaacgg aagcttcgca ttggaacgtt tgtataatag
     3001 ccattcccgg cactccatca atacaggaaa accaattcct ccgacaaaaa tcaaaaacat
     3061 gatcaggatc aagaaaaaat agtcatgtgc aaagggcttg atggaatccc cagtgacgtc
     3121 aaacccagag ttcgtgacag ccgatatcgc ctgataaaaa ccgtaaaaaa cggcatcacg
     3181 ccaacgatca aaatagcctc tgtaataaaa ataaatagaa aagaatgtcc caaaaagtaa
     3241 ttgaaaccag attaaaatag caaaagtgat ccgaatcagt cggacgatac cagacaaacg
     3301 cggttgattc atatccgtca tgatcagctg tcgctgtttc agtgtgatcc gtcgtttaga
     3361 aaaaatgaca aaagcagtag aaatcatcat gatccccaaa ccaccaactt gaaataatat
     3421 ttccagcagt ataataccgt tatcattgaa cacagaattg atatcaaaag tcgatagtcc
     3481 agtcacactc actgtactga ttgccatgaa cagcatgtcc acaaaagaaa catggctatc
     3541 tggttcgcga aaaatcggca tataaaatag aacgagtgat aagaccgtca tcaatatata
     3601 ataaaaaacg atgatttgga tcgatgagaa atgattggca gcaaacttct gtcctcgccg
     3661 ctgcagcctt cgaaagaata aatcaacatg cacagtacta tcctcctgac aatagtcgtt
     3721 tctaccagta tacaagggga attaggaata ctcaaagaaa ttatcaaaca gataacaata
     3781 cagctttcga ttaaaataga aatggcttat aataaggaaa atcctagaaa aaagaaggag
     3841 gaggaataac aaattgatta atgctcaaca aatcgtagaa aaaatgaaaa atcaaaaaat
     3901 aaattatgat acagttctga aaaaaatgat catgcaatgg gagaaagaag aagtaaagcc
     3961 taagatatta atacatacat gctgtgctcc ttgcagtacg cacacattgg aattcatgtc
     4021 tcaatatgca gagatcacgt tatttttttc aaattcgaat attcatccta aaagtgagta
     4081 tttgcgaaga ttacatgaac aaaaacgatt tgtagaccaa tttaatgaga aaactgacca
     4141 tcatgttgcg ctaatcgttg atgaataccg tccaaatgaa ttcaccaaaa tggttttaac
     4201 aaaccatctt gaaaacgaaa aagaaggtgg tgctcgctgc agtgcatgct tcaatatgcg
     4261 attagatctt gttgcgcaaa aagcgcaaga actaggatac gattactttg gaagtgccct
     4321 gaccatctcg ccaaagaaaa atgcagcttt gatcaatcag atcggaatgg atatccaaaa
     4381 gatttacggg acacaatact tgccaagtga tttcaagaaa aacaaaggct atgaacgctc
     4441 tttgcaaatg tgcaaagagt atgatattta tcgccaatgt tattgtggtt gcgtgttcgc
     4501 tgccaagcag caaggatgcg atctgaaaga aatcaatcgg gaagcaaaag cttacttaaa
     4561 aacatcaacc gtagtttttg aggaaaatta aagaaatctt tcggaaaaac gtaagaaaaa
     4621 aataaagaat ttatggtaga aataaggtag actgtataat ttgtccgtag taaagtaagg
     4681 aaacaagaat aaatttcaat gaaggaagag ataagatgcg aaaagtagca cctattttga
     4741 agccaatcgc tgtattaaca atctcgacga ttgtcgcgac tactacaggt attgtgaata
     4801 gttccagcta ccgtgcacca agttcaagtt ctagtactgt agaagaaaca acaccaacta
     4861 cttcagtctc gacatctgaa ccaacaacaa cggaaagttt gaatgaaacc actacgacaa
     4921 gtacatggga cgatacaaca actagttcgt cgtatgatga gacatataca actgacagct
     4981 caagcgaaac aagtaatacg gtaccagatg gcactccatc ggattcaacc aatccaagta
     5041 caagtccgga tacaagtgct agctccgaaa cgataccaag cagtacgact gtagatgata
     5101 acacgcaaaa tgatgatgca gcacgtacag cacagcaaaa ccaagattca ggaaataccc
     5161 aaacgtataa ccagcaagca ggtgattctc aatgacattt ttactagacc ttatcaacgg
     5221 ggtgcataaa ttccttggct atttagacat cagtcctaaa tatcttaacc gtggatatac
     5281 gattttaagt gtgattccca cgctttattt attgagaatt gtttatggac tttggcaaaa
     5341 ccaaaactat cttcaatttt tcttatatgg acttgcattt ctagtgcttg tttattttac
     5401 tgttttaaat gtattttatt actttttaga taaaaacacg aaagccgatg tcactcagct
     5461 ttttgtgaag tatttacctg atgaagcgtt caacatccag acagaagaat cgatagataa
     5521 tggaaattcg atcgataaag tgaatacaca agaggtcatg gtttcttatg acgaagatta
     5581 tcaattgaaa ttagccgaga atatgcggta tttgattgga aatggtgaga tcaaaacaaa
     5641 tgaactcggt cgcatggatg gatttctggt cgatagaaat acattgtatc cttattacta
     5701 tgtcaagaaa acaggagaga aagagtatgt tctaaatatt ggacgaagct actcggacat
     5761 ggaaaaagta gggaccattc gacttgactc aacagaagac acattggaac ctgtcggatt
     5821 gttcatcgtc ggcggtgatt ttgtcaaaga cgggttacgc tatcatgagc cttatcgtct
     5881 gaaattgatt gcaaaaaagg cacagtcgca ggaagaacca gtggtttata gtcgtagcca
     5941 aagaagaaga cattcataat gtgatgaaac gccgtgtatc agtggttgcg ataaaaacgt
     6001 ttattcggaa taagggggat gtgacacgtc ttttgtcacg ttcctttttt atataacgag
     6061 aaagataaga cgaacacatc acttattctt tcctttcatt tataaacatt gtagaataaa
     6121 gaaagtacaa cgaagtattg tgaaaaaacg taataaataa cgaggaaaat acaagtagga
     6181 ggtaagctgt gaaagaattt ttgaaaaatt gggggatttt gatccttgta ttagcggcta
     6241 ttttattagc tagagtgtac gtgtttacac cagttactgt aaacgggcat tcgatggacc
     6301 ctacattaag tgatggacaa cgattgattt catctaagat ctcaaactat gaacgaatgg
     6361 atattattac tacaaaggaa ccgggggatg aagaacggat gattgttaaa cggatcatcg
     6421 gtatgcctgg agatacagtc aagatggaaa atgaccagtt gaccattaat ggcaaaaagt
     6481 atgatgaacc ttatctggat gagttcaaaa aagaattttc agaagataag ttacaaggag
     6541 aatatgctta tagctcagga ttccaagcac aagcagaaag ttcatccacc tttacgagtg
     6601 actttgaata tactgtgcca aaaggaaagt atcttgttct aggcgataac cgcttgattt
     6661 caaaagacag ccgaatgttt gggttagtcg acaaagatat gatccaagga aaagttgttt
     6721 tccgttattg gcctctttca gaaattaaaa tcatgtgatg aaaaaataaa aagaagctgt
     6781 gacatgtttt ttgtcacagc ttctttttcc gaaaattcct tcttatttct tggagttgct
     6841 cacttttttt gcaatctcat tttaagcttt ttggctgatg gataagaaaa gcgaagcgtt
     6901 tttgttcata aatagctttt ccccacgatg gaaggtatga ctagtagcag agacaaagcc
     6961 cgttttttcc aatcgattat tcaattcttc ttggctaaag ccattatgga ctttcggatg
     7021 actaatttgt tcattcttgt caaagtctac gatgatcaat tgaccgccag gagctaaaat
     7081 ttcataaagt tttgtaagaa tattctccgt atcaggaatg tgtaaaagta caagtgatag
     7141 aaggatgaga ttagcttgga cagcaggaaa ttcgacagaa gcatcagcgt ggatcgttcg
     7201 gacatttttc agatctgctg cttggatctt ttcttctgcc attttcagca tagtttctga
     7261 agcatcggca atgatcagtt ctttgaatct ttccgcaagc ggtaaactaa caagcccagt
     7321 tccgcctccg taatccagca ggactttttg gtggctgtcg cgcggtagat aagtagttag
     7381 ttcttcgcga ataatcttgg ctaatgcttg acggtcaggg gaatcataat ggttagcgat
     7441 ggaatcaaaa atattttttg tcatcaaata gctccttacg taaaactttg aaattttaac
     7501 ttattatagc acgtgaaaag aaagaaagca tgctatactc aattcaaaaa gggagaatct
     7561 agaaagaata tgccgatatc gtttgtaaaa gatagagagg aaaaaggaaa gtgtgtccga
     7621 gaaatattac tagacttgcc tgagtggttt gggcttcctg aatcaacgga aaaatatatc
     7681 gaggaatcaa gtaaactacc tttatggtgt gaaaaaagaa aagaagaata tttaggcttt
     7741 atcactcttt cacagacaag tgaagatact gccgaaatct attctattgt atgggaataa
     7801 aaaaagacta tcatcaccaa ggattaggaa caaaactttt taaagaagca gaaggttacg
     7861 cagcaaaaca ttacaaatat ttgcaagtga agactgtaga cgaggggcac tactctatat
     7921 atgatcaaac tatttgtttc tacgaatcat taggattttc aaggttggaa gtttttccaa
     7981 atttatggga tgaatggaac ccatgtttag ttcttgtcaa aaaattggaa caaaagtgag
     8041 caatagattt aatactaatc tgtgcttgat gaaaattctc actgtaaaaa atgctgaaaa
     8101 tcaagtataa taccatagga acaaactgga aggtgaaaaa gagcatggca caactatttt
     8161 ttaagtatgg cgcaatgaat agcggcaaga cgatcgagat cctaaaagtc gcacataatt
     8221 atgaagaaca aaataagcca gttgtcttaa tgacaagcgg tattgataca agagatggtt
     8281 taggcgttgt ttcaagccga atcggactga aacgtaatgc gatgccgata tttgaagaaa
     8341 cagacgtatt tgacgtaatt cagaaaatgg aaagcaaacc ctattgtgtg ctgatcgatg
     8401 aagcgcaatt cttaaaaaag aaacatgtca ttgcttttac gagaattgtg gacgaattgg
     8461 atattccggt tatggcattt ggtttgaaaa atgattttcg aaacgagtta tttgaaggat
     8521 caaaatattt attgctttat gcagacaaaa tcgaagaaat gaaaacaatc tgttggttct
     8581 gccacaaaaa agcaatcatg aatttacatt atattgatgg aaaccctgtt tatgaaggcg
     8641 atcaagtgca aattggcgga aatgaagctt attaccccgt ttgtcgaaaa cattactttc
     8701 acccatcaat gattacagaa ggagaataag catgtacgat caattacaag caattgaaga
     8761 ccgttatgaa gagcttggag aacttttaag tgacccagaa gtgatttctg atacaaagcg
     8821 ttttatgcag ctttcaaaag aagaagcaaa cacgcgcgaa acagtcgaag tttatcgtcg
     8881 ttataaagaa gtagtacaag gaatcaccga tacagaagaa ctgcttggtg aaaaactgga
     8941 tgatgaaatg gctgaactag caaaagaaga attatctgaa ttaaaaaaag aaaaagaagt
     9001 cttagaagaa aaaatcaaga tcttgctttt accaaaagat ccaaatgacg ataaaaatat
     9061 catcatggaa atccgtggag cagcaggcgg agacgaagca gcattattcg ctggcgatct
     9121 tttcaatatg taccaaaagt atgctgaagc acaaggctgg aaaacagaag tgatggaagc
     9181 taacgttacc ggaatcggtg gatataaaga agtgatcatg atgatcaccg gagacaacgt
     9241 atattcgaaa ttaaaatatg aaagtggtgc gcaccgcgtc cagcgtgttc cttcaactga
     9301 atcgcaaggg cgtatccata cttccaccgc aacagttgtc gtgatgccag aagctgaaga
     9361 agtagagatc gatattgaag acaaagatat ccgtacagat atctatcatg cttcaggtgc
     9421 tggcggacag cacgtcaata aaacagcatc tgctgtacgt ttgactcact taccaacagg
     9481 gatcgttgtt gctatgcaag atgaacgttc tcaaatcaag aaccgtgaaa aagcaatgaa
     9541 aatcttgcgt gctcgtgtgt acgataagat ccagcaagaa gcacaaagcg agtatgatgc
     9601 caatcgaaaa tcagcagtcg gaactgggga tcgttcagaa cggatccgga cgtataattt
     9661 cccgcaaaat cgtgtgactg atcatcgtat cggattaact atccaaaaat tagatcagat
     9721 tttagcaggg aaattagatg agatcgttga tgcattgatc atgtatgatc aaacatcaaa
     9781 actggaagct atgcaaaatg ggtaaaatga cctatcgaga agtcctgtca cgggcttctt
     9841 cttttttaga ggatcatggg aaagaagcat atagtatcca atttttattt ttggaacgaa
     9901 aacaatggaa aaaacttgat tggctccttc atatgaacga agagatttca gaagaagaac
     9961 agcggttgat cgaaaccgat ctgcgacttc tgcttgcaga tcatccacct cagtatttgc
    10021 taggatatgc agatttttat gatcatcggt taaaggtaac tgaagcaaca ctgatccctc
    10081 gaccagaaac agaggagtta gtcgaatggt gtttagacga gacgcctggt gtaccgttag
    10141 aagtgataga tattgggaca gggactggag caattgctat tagtttgaaa gcagcaagaa
    10201 aaaattggca tatatctgct gtggatcttt cagaagaagc attagaagta gcgaaagaaa
    10261 atgctcagaa agaaggaacg aaaatcagtt tttatcatgg agatacatta ggaccagtaa
    10321 tggatcagca atttgatgtg attatttcta atccgccgta catcagtcgg aatgaatggg
    10381 aactgatgga tgaaagtgtc cggtcattcg aacctaaaat ggcccttttt gctgagaacg
    10441 atggattagc tatttatgaa aaaatagcag ttgaagcacc aagcgtacta aaatcaaatg
    10501 gaaagatttt tttagaaatc ggatttcgcc aaggtgaagc agtaaaaaaa atatttcagc
    10561 aagcatttcc agataaaaaa gtggcgataa aaaaggatct ttttggcaat gaacgaatga
    10621 tccaagttta tggttgaagt tgtggatgaa aaatttttta tttgtttttt tattataaaa
    10681 aaacacttat ttgacaagga tgtaaacgac tttttggttt atcaacaagt ttatccacta
    10741 ttgacaaata gattatccac aaacagtgga aaacttgagg ataaactcag acaaaataag
    10801 aatgtatgaa aaaggggttg tggataaact gtggaaacga aacgatatga tgaaacagaa
    10861 ttgcaagagg cagccgaagc actgaaagaa ggagaacttg ttgcttttcc taccgaaaca
    10921 gtatacggat tgggcgccaa cgctctttta ccaaacgctg tcaagaaagt gttttctgta
    10981 aaagggagac cgcaagataa tccattaatc gtccatgtcg cctcttttga acaagtaaaa
    11041 gagtatgtgg acaactttca tccggcagca gagaagattg tgaagaactt ctggcctgga
    11101 ccattaactt tgatatttaa gatcaaaaaa gacactttgc cctcagttgt gacgggagga
    11161 ttatccacag ctgcatttcg tataccggat aacaaaaaaa cactagaagt aatcgaactt
    11221 tccggtgttc cgcttgtcgg tccaagtgca aatacgtcgg gaaaaccaag tccaacgact
    11281 gcagatcatg tatatcatga tctacaagga aaaattaccg ggattattga tgatggagct
    11341 acgcgaattg gtgtagaatc cactgttctt gatttaagtg atccaacagc catgcctatg
    11401 attttacgac caggtgcagt aacgaaagaa caaatcgaag cagtcatcga atctcctgtt
    11461 gctattgatc agcatcttgt aaaagaaaat gaaacaccaa aagcccctgg gatgaaatac
    11521 aaacactact cacccgatac acgtgttctg atggtcagag atggcgattg gtcaactgca
    11581 gttcaatggg caaaaaataa gaagatccgt gcaggtgtca tagcaagccc cgaaattgcg
    11641 gatcaagtgc ggacagatac tgcagctgtt tatatgtata acgacaactc agtagaagcg
    11701 gcggctaaag gattgtttgc aggtctaaga ggattagatg aaccaacact tggattagat
    11761 ttgatttttg tacaagtgta tccagaaact ggattgggga atgcttacat gaatcgcttg
    11821 aaaaaagcag caggacaaaa ttattttgaa aaatgatcaa ttggttattg tcgcattctt
    11881 ttagtatgat acaatgctat caagtaaatc ttctatcagt gaacgaaaaa ctaagcgtaa
    11941 ctaatcgtaa tagaagagat acgaatattt ggcaatttgt ttttgatggg aaaaataaaa
    12001 ttgtaggaaa ataaaaagga gctgttgttt gtggtagatt acaaaacgtt tgaccctgat
    12061 ctttgggctg cgatcgccaa agaagaagaa agacaagaac ataatttgga gctgattgcc
    12121 tctgaaaact ttgtctccga agcagtgatg gctgcacaag gaagtatctt gacaaataaa
    12181 tacgcagaag gttatccagg gcatcgctat tatggtgggt gtgaatttgt cgatatcgtt
    12241 gaaaatttag cgattgaccg tgcaaaagaa ttattcggag cgaaatttgc gaacgtccag
    12301 cctcactccg gctctcaagc caacacggct gcttatctcg ctttggtaga accaggggac
    12361 acgatcttag gaatggatct ttccgcaggc ggacatctaa cacatggatc accagtcaac
    12421 ttcagtggca aaacctatca ctttgttgct tacggtgttg atccgacaac agaagttatt
    12481 gattataatg tggtacgtat tttagctaga aaacatcaac caaaattgat cgtagcaggt
    12541 gccagtgctt acggacgaac gatcgacttt gcgaaattca gagaaatcgc agatgaagta
    12601 ggcgcaaaac tgatggtaga tatggcacat atcgcaggat tagtagcagc aggacttcat
    12661 cctaatccag ttccttatgc agatatcaca acgactacca ctcataaaac attgcgtggc
    12721 cctcgaggcg gcatgatttt gacaaacgat gaagcattag caaaaaaaat aaatagtgcg
    12781 gtatttcctg gtattcaagg cggccctcta gagcatgtga ttgcgggtaa agcagtagca
    12841 ttcaaagaag cattagatcc tgcatttaaa gaatatagtg aacaaatcat cgccaatgca
    12901 aaagcaatgg tcaaagtttt caatcaagca attggaacca gagtcatttc aggggcgaca
    12961 gataatcatc tgatgttgat cgatgttcgc gagttaggta taaatggaaa agaagcagag
    13021 agcattttgg acagtgtgaa tatcactgta aataaaaact cgattccttt tgaaacacta
    13081 agtccattca aaacaagcgg tattcgaatc ggcacgccag caatcactac tcgcgggttc
    13141 aaagaagaag atgctgtaaa agtggctgaa ctagttgtca aagcattaca agcaaaagat
    13201 gataacgctc aactagacga agtcaaaaca ggtgtacgcg aattaactga aaaatttccg
    13261 ttgcataaaa aataaaaatt taaattccat tagtaaacag aaaagttcaa tgttgggctt
    13321 ttctgtttat tttttgggaa aacggattct ttgacaatat cggtgatgct ctctagtata
    13381 tgaggtgtaa ttttagtctt cacactaaat ctcagggagg aaaagtcatg actgatcgta
    13441 tctcgttaga aaaggaagca gtagcattca gtgatgcaaa tgcgcctcat ccgcgtatct
    13501 atgaactagc accggaagaa ggaagagttt tactagaaga agtacaagaa tcacctgtga
    13561 acaaatattc cgtcgatatt gaagacttga tggtggatac tggcgaatgg ggaaaactca
    13621 atattcgtct cgtacgacca gaagggaata tggataaatt accagttatt ttttatattc
    13681 atggagcagg ctgggttttt ggcagtgctc aaacccatga taaattgatc agagaacttg
    13741 cggttcgtac gaattccgtc gttgtctttc cagaatatag tcgatcacca gaagcaaaat
    13801 atccaacagc aattgaacaa aattatgctg tgttgcaaca gctgaataaa ttagccaagt
    13861 caaaaaatct ggatctcgag aatctaatcg ttgcaggaga ttcagtaggt ggtaacatgg
    13921 ctacagtcat gacgatctta acaaaagaac gaaagggcct cccaatatcc aagcaattgc
    13981 tgtattatcc agtcacaaac gctgattttg atacagattc ctatcatgaa ttcggagaaa
    14041 actatttttt gacaagagaa ggaatgatgt ggttttggga ccaatatacg acgaatgaag
    14101 aagagcgtgc tgaaatcaca gcttcgcctt tgagagcaaa tataaatgaa ctaaatgatc
    14161 ttccttcagc aatgatttta acgggagaag cggatgtatt aagagatgaa ggtgaggctt
    14221 atgcgcgcaa acttcgtgat gcaggggttc ctgttacaca agtacgcttc caaggaatca
    14281 tccatgattt tgtcatggta aatgctatgg atcagacaaa ggcaacgcgt gcagcaatgg
    14341 cattatctac ttcttggatc aagaaaaaag aataagcttt gcttgtccgc aggaaaactt
    14401 cttttcaaaa acggattttc ttaggaaaaa tggcgagttt gactagtcaa tcacttgatt
    14461 ttgttttaca attaaaaaga aacgacagaa caaaggagag cttaaaaaat gggcaaattt
    14521 caagtgatcg atcatccatt gattcaacac aaactaacta ttattcggga caaaaactgc
    14581 ggaacaaagg tttttcgtga agtagtcgat gaaatcgcta tgttgatggc atatgaagta
    14641 tcacgggata tgccattgga agacatcgtg atcgaaacac cgatcactga aacaacacaa
    14701 aaaacgttgt ctggtaaaaa agtagcgatt attccaatct tgcgcgcagg tattggaatg
    14761 gttgacggta tcttggaatt gattccagct gctaaagtgg gacatatcgg tctttatcga
    14821 gatgaagaaa cattggaacc acatgaatat ttcgtcaaac ttccagaaga cattgatgca
    14881 cgtcagttgt ttgtagtaga tccaatgttg gcaacaggcg gatcagcaat catggcaatc
    14941 gatgcattga aagaacgtgg cgcgtcgaac attaaatttg tttgtttggt agcagcacct
    15001 gagggagtaa aagctttgca agaagcgcat cctgatattg acatctatac agcttctcta
    15061 gatgaaaaat tgaatgaaca tggttatatc gttcctggac taggggatgc gggtgaccgt
    15121 ttgtttggta caaaataagt aaatacgaaa aagggtgtga cacaactttt gtcacatcct
    15181 tttttggtca tttttaacgt ttgatgcaga acagcttttt cacagctttc gtttgcatat
    15241 aaaaaaacca tcccaaaaat gcagagatgg tcaaattcaa atctttttta gttctttgta
    15301 gttaacgatt actacaccct tactttgcgt catcaagttc gcattttctg cagtttcatc
    15361 aatctcaaca acagcggaat ttttcaattg ctttgaaatc gttccagttt gcgacttgcc
    15421 gtgaattgtg aacattacgt gagtattgat cttgaatttt tcaccaattt ccgatggtac
    15481 gatagttgta tcaaattttc caaaagttgc cattttcata aaacctgcct ttcacatcaa
    15541 ttataccacg ttttatgaaa aatatcatga aattaaacta attttccatc ggactcttta
    15601 caatcagcca aatagagtat ataatttctt ttggataagg ccagcttagc tcagttggta
    15661 gagcaacgca ctcgtaacgc gtaggtcgta ggttcgatcc ctatagctgg cattaaaaga
    15721 gaagacagat atatcaagga ttttta
//
LOCUS       DAZKKW010000061        15230 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.062, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000061 DAZKKW010000000
VERSION     DAZKKW010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15230
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.062"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            278..787
                     /locus_tag="ACG8PO_001891"
     CDS             278..787
                     /locus_tag="ACG8PO_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFQ0207198.1"
                     /translation="MVQGIERGVYVVDKIQFNQVIEEFIKVNNLMTHIQKRPIRITND
                     LKLSTSMIHLIDVIGNYPETSITELADRLGVTKGAVSQQIPTLEKMNLISISQKKGNK
                     KTKLVSLTKYGRKVYESHNYLHEELYSSINEALESFSPDQISTIKQILEKVSYTINEY
                     QVSLTRKED"
     gene            880..2042
                     /locus_tag="ACG8PO_001892"
     CDS             join(880..1156,1156..2042)
                     /locus_tag="ACG8PO_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFQ0207199.1"
                     /translation="MSRRQRRTYSKEFKQQVVDLYLAGKPRAEIIRDYELTTSAFDKW
                     VKQAQTTGSFQEKDNLTPEQVELIALRKKNKQLEMENDILKQAALILGPKRQVIDTNK
                     HKYSISAMCKVLNISCQTYYYQAKPVEDESELEELVQEEFIRNRKAYGTRKLKQCLAN
                     LGLQVSQRRIGRMMKQRGLRSTYTLAHFKFHSSTCNEAKTANILDGTFLQEKSLEAIV
                     TDLTYVRVGKKWQYICLILDLFNREIIGYSCGEKKDATLVKEAFAKIPYSLNNVQLFH
                     TDRGKEFDNQTIDEILWAFNITRSLSKKGCPYDNAVAESTYKSVKVEFVHQYRFETLA
                     NLRLELFDYVHWWNTIRLHGTLGYETPISFRQQRLAQRILDNELGCDTGGEAP"
     gene            2322..3302
                     /locus_tag="ACG8PO_001893"
     CDS             2322..3302
                     /locus_tag="ACG8PO_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0207200.1"
                     /translation="MKKIGIREVAKEANVSPTTVSRVLNDRGYLSEETKRKVFDAMKK
                     LEYYPNELARALFKNETYTVGLIFPTITNPFHAELIQDIEFELSSRGYKVLLCNSLNN
                     PTKEKEYLTMLRKNQVDGIIVGTHNKDIADYDIPGLPIVAIDRSLGENTVTVSCDNYR
                     GGELATQLLVNHGCQNILCIRGDSKIKLPANSRTLAYKDVLNKFMLEPLVLEVPFVKE
                     INEKNEIIKTYLETHPNIDGVFAGDDLLASLAINNLGSSGKRVPKEVKVIGFDGAQQT
                     LIYNPELSTIQQPIDQISKVAVKKLLNMIKGEKETDELSLPVKLIKKNTA"
     gene            3424..4842
                     /locus_tag="ACG8PO_001894"
     CDS             3424..4842
                     /locus_tag="ACG8PO_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HFQ0207201.1"
                     /translation="MEKLLIKSNNHQLYYQPKDVWVGDVMPYGKEGKFFIYHQRDTRN
                     PVPFGEPFGWALATTKDFVHFEDFGESLLRGDDNERDQFIYAGSVFEANGKVHAFYTG
                     YNREFLAQGKTSQVLLHAESDDFVHWEKSETALELEPQEGYDIRNWRDPFVVWDDEKE
                     EYLLILGARKGQDKHLQTGRLVEFTSKDLTNWEFKGDFWAPGLYTMFEMPELFKIGDW
                     WYLVYSEYSDGNKILYRMSQSLEGPWIKPKDEAFDGRAYYAGRTAYDGKRRVLFGWVP
                     TKDQENDKNNYLWGGTLVPHEVYQKEDFSLGVKPIDEVWNAFINLHDIADVEIETADT
                     REEVTLVKNSGTLFNLETTISFDENLSDFSLRLYKNLETDESYEYRFNLDEGRLTFDK
                     NPCYPWFQYMDKGLIRPISLKPNQSYKLKVIVDDNIMTLYIDGIALNARVHGCFGQAI
                     TITATNGSMTAINTKFSNEIKK"
     gene            4913..6382
                     /locus_tag="ACG8PO_001895"
     CDS             4913..6382
                     /locus_tag="ACG8PO_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFQ0207202.1"
                     /translation="MDYKKIAEDVLENVGGKENISSATHCITRLRLILKDKSKANMEM
                     LEEIEGAKGVLFNSGQLQVIFGTGIIEKVYKEFVELTGAKEVSVSEMKDEGVNNSGNR
                     LQRAFKVFSDIFIPIIPAFIGAAMVLGIRSLITTPGMFGMDGSFADHNVFLNDLSKFL
                     GIIATTFDYLPVLVMYSATKRFGGNPILGLLVGFVMIHPGLANRNDFVMGTIKPDYWH
                     LFGLSIPAVAFQGGVFPAILTSWFLAKVEKITNKYSPQVLAYILVPTVTIVLANFALF
                     LVFGPLGNFIGNVLGGIIDFLYMKMGAFGAFVFAGALQPLVVTGTQHAIQGIEANLVA
                     TTGFNYIQPIWSVSIIAQGGGCIGMYLLFKKKSKDRDIAISSFIPTLVGITEPAIFAV
                     NLKYSIVPFVCSFIGAGFGGAFMKIFDVKAIGQGLTVLPGLTIANPVLPYIFGNLIAF
                     ILPIIFILAYDKYKVFIPEAKHRKFAEKTDSKKLPVQVK"
     gene            6392..6859
                     /locus_tag="ACG8PO_001896"
     CDS             6392..6859
                     /locus_tag="ACG8PO_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFQ0207203.1"
                     /translation="MLNFFRKNEISVVAPATGVFEKLEKLSDPVFSKGMMGQGFAIDA
                     KNETIVSPIDGFVSSIFPTKHALGIKTKSGIEILVHVGIDTVELNGEGFDIKVQEGQK
                     VKAGDILMTINFVVFEKNHKTKDVIVVFPDYQDEIFIPKLEEVKQGQELFVLG"
     gene            6895..7767
                     /gene="scrK"
                     /locus_tag="ACG8PO_001897"
     CDS             6895..7767
                     /gene="scrK"
                     /locus_tag="ACG8PO_001897"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008865 - fructokinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase ScrK"
                     /protein_id="HFQ0207204.1"
                     /translation="MVFGAIEAGGTKFVCAIGDKDLNIFEKVSFPTTTPKETMNLVLD
                     FFNHHKDDLEGIGVGSFGPIDIHCESKTYGFITSTPKLAWQDFDFIGSLKKQLNIPTA
                     WTTDVNASCYGEYVSGNGRGLSSVVYYTVGTGIGGGAIQNGNFIEGFSHPEMGHMLVK
                     RHNKDNFSGTCPFHGDCLEGLAAGPAIERRSGKKGQEVTENDVTWEIEAYYIAQCAYN
                     TTLMLSPDVIIFGGGVMKQKHLKKKVQDKFIELINGYVAIPDVDNYIMTPKLLDNSGI
                     VGCLALARDVKEKS"
     gene            7849..10383
                     /locus_tag="ACG8PO_001898"
     CDS             7849..10383
                     /locus_tag="ACG8PO_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFQ0207205.1"
                     /translation="MKKKMKDEVLEYIKNNLKYYDFSAQDIAMKFCIKRNVASHYLNQ
                     LFSDGKLLKNDSVRPVMFKYNQQKPKDCFSKFIGADISLKSTIDKCKATVMYPPNGLP
                     LIIKGNSGVGKSFLASLIYQYALDRKVIHNDAKFVVVNCADYANNPELLSAVLFGYKK
                     GAFTGAEKDTAGMLSSANGGYLFLDEVHNLSAENQEKLFLLMDSQKYRKLGESVQWEY
                     ANVRLILATTEDKNTSLLATFRRRIPAEITLPDYNNRSTSEKIQLLFEFLKDEAITID
                     SKIFCSIELFTDLLSKTFEGNVGELRNEIKLLCAEGYLGNKRKSSIYLGDKLDSGFWI
                     DPEISIDLKKMFLKSLSEIDLIELFQNHISIEESMSQLRDRILKECAGSSEYNYLESD
                     EFIALRNYVVNKISPIIDSTGLCLLDEFLADISLFIMFIDVIEETSRNFLLSKFRKFR
                     MKNDKQKLLANEIWSSLEVEESKQELLLKWILFLVKKFYVKIPETHCLIVMHGKITAS
                     AIARETNKLLNTYVYEAFDMPIEGETSDLINLINNFCKSIDTTNGLILLVDMGSLEQM
                     YEKIESNVIGDLVILNNVSTALAIECGIQVCQKKPISHFYQMDFDSFQVKVQYYKGLS
                     QKKNVIVSCLSGEGISEKVKDILKRYLDNQVEILIFDFNALKKIAKEKDTMIFKNTIC
                     VLSTTEFYIQGIDCLNLENLINGNQTLEKLNKYMDSDSCEFCLNELVKLFTIEGASTK
                     LRFLDSKKVFNEIEKVLYLYEKYYDVKIPSFLRINLFLHLSGMIERIMIGDGITNEHL
                     KEGAQLFDTFLNVSKDFFSEIKDIYCIEIPKGEYELIYTIFEQTIF"
     gene            10539..11312
                     /locus_tag="ACG8PO_001899"
     CDS             10539..11312
                     /locus_tag="ACG8PO_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFQ0207206.1"
                     /translation="MAFWQTFLVVLYGFFINWDKEGPKIGTSQPVVAGFLTGLIMGDI
                     QTGLYIGGTLQLMTLGITSFGGASVPDYQTAALIGTYLAISTNQDASIGITLAIPVAM
                     LIVQLDVLKWSTNIFFQQKAEKFAQKEDYQKLEWMQYLASINTSFVSGIPVLLTVILG
                     PTLVGSAINYIPEWVTGGLKVAGGLLPAVGIGMLLKYLPTKDYFAYLLVGFVLAVYFQ
                     IPILGISLLGAAIALIVYKNRSNNTQVAPAATGGFDEDE"
     gene            11305..12096
                     /locus_tag="ACG8PO_001900"
     CDS             11305..12096
                     /locus_tag="ACG8PO_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFQ0207207.1"
                     /translation="MNKKLTKIDKQVIFRWILNGATSLNYEKMEGLGYAYSMLPFIKD
                     TYKDDPEGMKQSVLAHLQFFNTTPYTAPYIIGMNVGIEKEEKNKSLEAVSALKTGLMG
                     PLAGIGDSVFVVIPWTIFGAIAANMALEGSFVGIILWILASILLRITAYPLYLAGKES
                     GASLLTQIETKLKSITDAVSILGLMVVGALIATVVKVNVGFEFKQGDLTMTGNEIFDQ
                     VMPGLLPACVVAIVYYLLGRKVKPVYVILLILVVSIALRALNILK"
     gene            12106..12597
                     /locus_tag="ACG8PO_001901"
     CDS             12106..12597
                     /locus_tag="ACG8PO_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFQ0207208.1"
                     /translation="MDEKMKGIIHVRIDDRMIHGQVATQWTNQLSANRIMVINNEVAN
                     DDVKKAVVRLAAPPNVRTSIITREVAVKNILSGKYEGQKVLIVAVSPIDVKFLIDNGL
                     PITSVNVGNLSRRNGTERIRPTINITEEEREAFKELISDGVEVTVIQTPRDSKEFLKD
                     FIY"
     gene            12615..13025
                     /locus_tag="ACG8PO_001902"
     CDS             12615..13025
                     /locus_tag="ACG8PO_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFQ0207209.1"
                     /translation="MEFIITSHGNIAIGFVDTLKMFFGDNLNCHVVTLGDAGIEEFRE
                     NIEKIVSQVKNQEVMVFADLIGGTPFNEFAKRSEVFQNGFQLLGGMNLGILIEAVNLR
                     LQGKKSDDIIENLRNMNTIACLADMQRHTNEEDE"
     gene            13029..14429
                     /locus_tag="ACG8PO_001903"
     CDS             13029..14429
                     /locus_tag="ACG8PO_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-fructofuranosidase"
                     /protein_id="HFQ0207210.1"
                     /translation="MQKIFYKPKDFWFGDCMPFYKDGTFYLFHQRDTRNPKPLTDPFG
                     WSLVTTTDFVKFEEYGEVIHRGGNDDIDQYIYAGSIVQGINEYLALYTGHNRKAKLDG
                     KTSEVLMIASSKDLIHWKKDGEAAKLVPQEGYDKNDWRDPFAIFDQDHGKYVLILGAR
                     KPASKKKPTGRLVYFESEDLKDWNFKGDYWANNEFNMIEMPDIFEIDGTWYLLFSEYC
                     EDKKTKYRVGAGLFSDWKSPVDEAFDGKAYYAARTVGTNETGRFLTGWVATKEGENDQ
                     NNWDWGGALVPHQIIQKTDKTLGVKLPEKIIENFKEITPFSSFKLEKEFGKKERVIVP
                     KIEDYFLFETTLTITSLTKEFALKLFVDSDTQEGYEFRISLINNQLTFGKTPNYPWPQ
                     MFDKGLQRPLFLEIGKEYELKLIVDDTIAVLYIDNVALSTRMYDKVGTALSMTVTEGA
                     IKVDKLDYSINYECGF"
     gene            14452..14826
                     /locus_tag="ACG8PO_001904"
     CDS             14452..14826
                     /locus_tag="ACG8PO_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFQ0207211.1"
                     /translation="MKLNGLQHIGIPTKDFGASKIFYESLGFSLINEEMNVGSKVGFF
                     ELNGTIIEIWEDKTTECRGAIDHLALDTDDIDEVFNSIQDLGYELLDKNIMELPFWEN
                     GIRYFNFYGPNHEIIEISQMKK"
BASE COUNT         5286 a         2138 c         3045 g         4761 t
ORIGIN      
        1 ttttttattt gtctcgctta attatacaaa cggcgtgata aaaaacgaga aatgaattat
       61 aggtttagac ctttatcatt tctcgtttta ttttattgta tcaaggctct atcataagtg
      121 gtaaaatcta cttttaaatc acatgtaatc attgataagt ccagtgtttt agcggataat
      181 cagcttttta tttatttttt cttataaatt tgataaatag aaattagtat agccctaaac
      241 aaattatttg tgttttattg tttagggcta tactatattg gtacaaggaa tagaaagagg
      301 tgtttatgtg gttgataaaa ttcagtttaa tcaagttata gaagaattca tcaaagtaaa
      361 taatttaatg acccatattc aaaaacgacc aatcagaata actaatgatc taaaactcag
      421 tacgagtatg attcatttga ttgatgtgat tggaaattat cctgaaacta gtattacgga
      481 attagctgat cgattaggcg ttaccaaagg agctgtatcc caacagattc ccactttaga
      541 gaaaatgaat cttataagca tttctcaaaa aaaaggaaat aagaaaacta aacttgtatc
      601 gttaacaaaa tatggtcgaa aggtgtacga atcacataat tatttacatg aagagcttta
      661 tagctcaata aatgaagcgt tagaatcatt ctcaccagac caaatttcaa ccattaaaca
      721 gattctagaa aaagtatctt atacaatcaa tgaatatcaa gtaagtctga caagaaagga
      781 agattgacat gaaaaaaatg atttttgaat cggctacact agactagaca gaaaaaataa
      841 ggtgtgtaaa ataaatgaaa acacactgga ggaattttta tgtcaagacg tcaacgtaga
      901 acctattcaa aagaattcaa acaacaagtc gtcgatcttt atctcgcagg aaagccccga
      961 gctgaaatca ttcgagatta tgagctgact acctcggcct ttgataagtg ggtcaagcaa
     1021 gcgcaaacta ctggctcgtt tcaggaaaaa gataacttga cccctgaaca agtagaacta
     1081 attgcattac gaaagaaaaa taaacagctt gaaatggaga atgacatttt aaagcaagcg
     1141 gcgctgatat tgggacgaaa agacaagtaa ttgataccaa taagcacaaa tactcaatat
     1201 cagcgatgtg taaagtgtta aacatctctt gccaaaccta ttattatcaa gccaaaccag
     1261 ttgaagatga atcagaactg gaagaactcg ttcaagaaga atttattcgc aatcgaaaag
     1321 cttatggtac caggaagctg aagcagtgct tggccaatct aggtcttcaa gtgagtcaac
     1381 gcagaatcgg ccgtatgatg aaacagcgtg gcttgcgttc aacatacaca ttggctcatt
     1441 tcaaattcca ttcttctact tgtaacgaag ccaagaccgc caatattcta gacggaacct
     1501 tcctacagga aaagtcatta gaagcgattg taaccgacct tacctatgtt cgtgttggga
     1561 agaaatggca gtatatctgc ctgattcttg atttgtttaa tcgagaaatc atcggctatt
     1621 catgcgggga gaagaaagat gcgacgttag tcaaggaagc ttttgcaaag attccgtact
     1681 cgttgaataa tgtacaactt tttcacacgg atcgtggaaa agaattcgat aaccagacga
     1741 ttgacgaaat tttatgggcc tttaatatca cgcgttcctt aagcaaaaaa ggctgcccct
     1801 atgataacgc cgttgcagaa tccacctata aatctgtcaa agtagaattc gttcatcaat
     1861 accgctttga gacattggct aatttgcgat tagagctatt tgattacgtc cattggtgga
     1921 ataccatccg gctgcacggc acgttaggct atgagacacc gatcagtttt cgacagcaga
     1981 gattggcgca gcgaatcctt gataatgagc tcggatgtga taccggtggc gaggcacctt
     2041 aattatttga atgagcttct gccttagaga atcagattcg aggaggctcg ttgtcaaggg
     2101 cagtcggaac accagcagca agcttacgca ataccttata atttttgtca aaaaaagtgt
     2161 tgccattcca catatatctt taaacagcta ttgccagata aaaataaatg atattatata
     2221 tataaatact tatccgaaaa ggggaatgag agagtcaagg ttcttggaat gtcgaatagt
     2281 cgagtaggta gattttctat ctacaacctt gttgtttaaa aatgaaaaaa attggaatta
     2341 gagaagttgc gaaagaggca aatgtttctc ctacgactgt gtcgagggta ttgaatgatc
     2401 gtggatattt gagtgaggaa acaaaaagaa aagtttttga tgccatgaaa aaattagaat
     2461 actatcctaa tgaactggcg agagcacttt ttaaaaacga aacatatacc gtaggtttga
     2521 tttttcctac aattactaat ccatttcacg ctgagttaat tcaagatatc gagttcgagc
     2581 tttccagcag gggatataaa gtgttgctgt gcaatagctt aaacaatcca acaaaagaaa
     2641 aagagtactt gacgatgttg agaaagaatc aggttgatgg aattatagta ggaacgcaca
     2701 ataaagatat tgcggattat gatattccag ggctacctat tgtagcgatt gatagaagtc
     2761 ttggtgaaaa tacagttaca gtatcttgtg ataattacag gggtggggaa ttagctactc
     2821 aactacttgt aaatcatggc tgtcagaata tactgtgtat tcggggagat agtaagataa
     2881 agctacccgc gaatagtagg actttagcat ataaagatgt tttgaataaa ttcatgctgg
     2941 aaccgttagt cctagaagta ccatttgtaa aagaaatcaa tgaaaaaaat gaaataatta
     3001 agacatatct ggaaactcat ccaaatattg acggggtttt tgcaggagat gatttattgg
     3061 cttctttggc gattaacaac ttaggaagtt cgggcaaaag agttccaaaa gaggttaaag
     3121 tgattggatt tgatggagct caacaaacat tgatttataa tccagaatta tcaactattc
     3181 aacagcctat agatcaaata tctaaagtag cagtaaaaaa attattgaat atgattaagg
     3241 gcgaaaagga gacggatgaa ctttcacttc cagtaaagct cataaaaaaa aatacagcat
     3301 aagttaaatc ggcgaaaaat tccttcgtcg atttaaaaat atttttatga aacgggttga
     3361 catatgaaac gggttgacat atattagttg tgaagataaa aaccttttca ctggaggaag
     3421 aaaatggaga aattattaat taaaagcaat aatcatcaac tttactacca acctaaggat
     3481 gtttgggtcg gagatgttat gccttacggg aaagaaggaa aattctttat ttatcatcaa
     3541 cgtgatacta gaaatccagt tccttttgga gaaccgtttg gctgggcttt agcaactacg
     3601 aaagattttg ttcattttga agactttggg gaatctcttt taagagggga tgacaatgag
     3661 agagaccagt ttatctacgc tggttctgta tttgaggcaa atggaaaggt tcacgcattt
     3721 tatacagggt ataatcgtga attcttagcg cagggaaaga catctcaagt gttgcttcat
     3781 gctgagagtg atgattttgt tcattgggaa aaatcagaaa cagcattgga gttagaacca
     3841 caagaagggt atgatattcg aaactggaga gatccatttg tcgtttggga tgatgaaaaa
     3901 gaagaatatt tgctaatttt aggggcgcga aagggtcagg ataaacatct acaaaccgga
     3961 cgtttagtgg aatttacatc aaaagattta actaattggg aattcaaagg tgatttttgg
     4021 gctccgggat tgtataccat gtttgaaatg ccagaacttt tcaagattgg tgactggtgg
     4081 tatcttgtct attcagaata ctcagatgga aataaaatac tctatcgaat gagtcaaagt
     4141 ttggaaggtc cttggattaa gcctaaagat gaagcttttg atggacgggc ttattatgca
     4201 ggtagaacag cttatgatgg aaaacgtcga gtcctatttg gatgggtacc aacaaaagat
     4261 caagaaaatg ataaaaacaa ttatttatgg ggaggtactt tggttcctca tgaagtctat
     4321 caaaaagagg atttttcact tggggtaaaa cccatagacg aagtgtggaa tgcattcata
     4381 aatttacatg atattgccga tgtagagata gaaacagcag acacaagaga agaagtaacg
     4441 cttgtcaaaa atagtggaac tttgtttaat ttagaaacaa caatttcatt tgatgagaat
     4501 cttagtgact tttcactgag attatataaa aaccttgaga cagatgaatc ttacgaatat
     4561 cgcttcaatt tagatgaggg gcgtcttaca tttgataaaa acccttgtta cccttggttt
     4621 caatatatgg ataaaggact tattagacct atatctttaa agcctaacca atcttataaa
     4681 ctaaaagtga ttgttgatga caatataatg acactttata ttgatggaat cgctcttaac
     4741 gctcgtgtac atggttgttt tggtcaagca attactatta cagcaacaaa tggatcaatg
     4801 actgcaataa ataccaagtt ttcaaatgaa ataaagaaat aataaacgtc aatcatgtgg
     4861 caggtctgaa aatcaaacat gacctgtcac atttaaaagg gaggataaat gaatggacta
     4921 caaaaaaatt gccgaagatg tattggaaaa cgttggagga aaagagaata tctcaagcgc
     4981 tacacactgc attactcgtt tacgtttaat tcttaaagac aaaagcaaag cgaatatgga
     5041 aatgcttgag gaaatagaag gtgcaaaagg tgttcttttc aatagtggac agttacaagt
     5101 aattttcgga acaggtataa ttgaaaaggt ttacaaagag ttcgttgaat tgacaggagc
     5161 taaggaagta tccgtttctg aaatgaaaga cgaaggagtc aataattcag gaaatagact
     5221 tcaacgagct tttaaagttt tctcggatat ctttattccg atcattccag cttttattgg
     5281 ggcagcaatg gttttaggaa ttcgttcgtt gattaccact cctggaatgt ttggaatgga
     5341 tggctcattc gctgatcaca atgtattcct aaatgattta tccaaatttc tgggtattat
     5401 tgcaaccaca tttgattatt taccagtttt agttatgtat tctgcaacaa aacgttttgg
     5461 cggaaaccct attttaggat tacttgtagg atttgttatg attcatccag gactagccaa
     5521 tcggaatgat ttcgtcatgg gaactataaa gcctgattat tggcatttat ttggattaag
     5581 cattccagca gttgcgtttc aagggggtgt ttttccagca attttgactt cttggttttt
     5641 agcaaaagtt gagaaaatta caaataaata ttctcctcaa gtacttgctt acattttagt
     5701 cccaactgtg acaattgttc ttgccaactt tgcactattt ttagtgtttg gtccattggg
     5761 gaattttatc ggtaatgttt taggtggaat cattgatttc ctatatatga agatgggtgc
     5821 ctttggtgct tttgtttttg caggcgcgtt acaaccatta gttgtaacag gaacacaaca
     5881 tgctattcaa ggtattgagg caaacttagt cgcaacaact ggattcaatt atatccaacc
     5941 tatttggtca gtttcaatta ttgcccaagg tggcggttgt attggtatgt atttactctt
     6001 taagaaaaaa tctaaagata gagatattgc gatttcaagc tttattccaa cactggtagg
     6061 tattactgaa ccggctatct ttgctgttaa tttgaaatat tcaatcgttc cttttgtttg
     6121 ctcgtttatt ggtgctggtt ttggtggagc atttatgaaa atttttgatg taaaagccat
     6181 tggacaagga ttgactgtat tgccaggtct gactattgct aatccagttc ttccttatat
     6241 atttggtaat ctcatagcat tcattttacc aatcattttt attctcgctt atgataaata
     6301 taaagtgttt atccctgaag ctaaacaccg taaatttgca gaaaaaactg actcaaaaaa
     6361 attgccagtt caagtaaagt gagatgagag catgttaaat ttttttagaa aaaatgaaat
     6421 aagcgttgta gcacctgcaa ccggtgtttt tgaaaaactg gagaaattaa gtgatccagt
     6481 cttttcaaaa ggaatgatgg gacaaggatt tgcaattgat gcaaaaaacg aaacgattgt
     6541 atctccaatc gatggttttg tttcaagtat ttttcctaca aaacatgcct taggaataaa
     6601 aacaaaatca ggaattgaga tacttgttca tgtaggaata gatacagttg agctaaatgg
     6661 tgagggattt gatattaaag ttcaagaagg acaaaaagtt aaggctgggg atattcttat
     6721 gaccattaat tttgtagttt ttgaaaagaa tcacaagact aaagatgtca ttgttgtttt
     6781 tccagactac caagacgaaa ttttcattcc gaaacttgaa gaagtaaaac aaggacaaga
     6841 attgtttgtt cttggctaat gataagtaat taataataga atcgaggaaa atgtatggtt
     6901 tttggggcta ttgaagctgg tggaacaaaa tttgtttgtg ccattggaga taaagatttg
     6961 aacatatttg aaaaagtcag ctttcccacg actacaccca aagaaacaat gaatttagtt
     7021 ttagattttt ttaatcatca taaagatgat ttagaaggta tcggagtggg atcgttcggt
     7081 cctattgata ttcattgtga atctaaaacg tatggcttca ttacatcaac gccaaaactt
     7141 gcttggcagg attttgattt tattggaagt ttaaaaaagc aacttaatat tccaactgct
     7201 tggacgactg atgtaaatgc ctcttgctat ggtgaatatg tttctggaaa tggcagaggc
     7261 ctttcaagtg ttgtttacta taccgtagga acgggcatag gaggtggcgc tatccaaaac
     7321 ggaaacttca ttgaaggttt tagtcatcca gaaatgggac atatgcttgt aaaacgacac
     7381 aataaagaca acttttctgg gacatgtcca ttccacggag actgtttaga gggcttagct
     7441 gctggtccgg caatcgaaag aagatcaggg aaaaagggac aagaagtgac tgaaaatgac
     7501 gttacttggg aaatagaagc ttattacatt gctcagtgtg catataatac aacattgatg
     7561 ttatcacctg atgtcattat atttggtggc ggagtaatga aacaaaaaca cttaaaaaag
     7621 aaagtacaag ataagtttat tgagttgatc aacggatatg tcgctattcc cgatgttgac
     7681 aattatatta tgacaccaaa attgttagat aattctggta ttgtaggttg tttagcttta
     7741 gcaagagacg ttaaagaaaa atcttaattt ggaaaaaaga tcgtctcgac tatttgtttg
     7801 gttgatgcga tctttttttt caattagtaa ttgaagggac gcctaaaagt gaaaaaaaaa
     7861 atgaaagatg aagtattaga gtatatcaaa aataatttaa agtattacga tttttctgct
     7921 caagatattg caatgaaatt ttgtattaaa agaaatgtcg cttcccacta tttgaatcag
     7981 ttattttcag atggaaaatt actaaagaat gattctgtta gaccagtaat gttcaagtat
     8041 aatcaacaaa agccaaaaga ctgcttttct aagtttattg gagcagacat atctttgaaa
     8101 tccactatag ataaatgcaa agctactgta atgtatcccc ctaatggcct cccattaata
     8161 ataaaaggaa acagtggcgt tggaaaaagt tttttagcaa gtttgatcta tcagtatgct
     8221 cttgatagaa aagtaattca taatgacgca aaatttgttg ttgtgaattg tgcagattat
     8281 gcaaataatc cagagctatt gtcagcagtt ttatttggat ataaaaaagg agcctttact
     8341 ggggctgaaa aagatactgc agggatgcta tcttctgcta atggaggata ccttttttta
     8401 gatgaggttc acaatctaag tgcagaaaac caagagaaat tgtttcttct tatggattct
     8461 caaaagtatc gtaaacttgg tgaaagtgtg caatgggagt atgcaaatgt tcgccttatc
     8521 ttagccacaa cagaagataa aaatacctct ttgttagcaa cttttagaag acgcatccca
     8581 gctgaaatca ctctgccaga ttacaataat agaagtacat ctgaaaaaat tcaattatta
     8641 tttgaatttc ttaaagatga agcaattacg attgattcaa aaatattttg ttcgattgaa
     8701 ctttttactg atcttttatc gaaaacattt gaaggaaatg ttggagaatt gaggaatgaa
     8761 atcaagcttc tttgtgcaga aggttattta ggaaataaga gaaagtcttc aatttattta
     8821 ggggacaaac tagacagcgg tttttggata gatccagaaa ttagtataga tttaaaaaaa
     8881 atgtttctta aatcactttc tgaaattgat ttgattgaac tttttcaaaa tcacatttca
     8941 attgaagaaa gcatgagtca attaagagat agaatactta aagagtgtgc aggttcttct
     9001 gagtataatt atttagaaag tgacgaattt atagcattaa gaaactatgt tgttaataaa
     9061 atatcaccaa ttattgattc gacaggtcta tgtttattag atgaattctt ggcggatatc
     9121 agtctattta taatgtttat tgatgtaatt gaagaaacat caagaaattt tttgctgtct
     9181 aagttcagaa aatttcgtat gaaaaatgat aaacaaaaat tattggcaaa tgagatttgg
     9241 agtagtttag aagtcgaaga aagtaaacaa gaactcttgt tgaaatggat actttttctt
     9301 gtaaagaagt tctatgtgaa aataccagaa acccattgtc tgatagttat gcacggtaaa
     9361 ataacagctt ctgcgatcgc aagggaaaca aataagttgc tgaatacata tgtttatgag
     9421 gctttcgata tgccaattga aggtgagact agtgacttaa ttaacctaat caacaatttt
     9481 tgtaaaagta tagatacgac gaatggactc attctgctag ttgatatggg atcattggaa
     9541 caaatgtatg aaaaaattga atccaatgta ataggtgatt tggtaatttt aaacaatgtt
     9601 tctacagctt tagcgattga atgtgggatt caagtttgcc aaaaaaagcc aattagtcat
     9661 ttttaccaga tggattttga ttcgtttcaa gttaaagttc agtattataa aggattatca
     9721 caaaagaaaa atgtaatagt atcttgtctc tctggtgagg ggatttctga aaaagttaag
     9781 gatattttaa aacggtatct cgataatcaa gtggagattt tgatttttga ttttaacgca
     9841 ttaaagaaaa tagcaaagga aaaagatact atgattttta agaatacaat ttgtgttctt
     9901 tcaaccactg aattttacat tcaaggaatc gattgtttaa atttagaaaa tctaattaat
     9961 gggaatcaaa ccttagaaaa attaaataag tatatggact cggatagctg tgagttctgc
    10021 ttaaatgaat tagttaaact ctttactatt gaaggcgcta gtactaagct tcgcttttta
    10081 gattccaaaa aagtattcaa tgagatagaa aaagtactat atttgtatga aaaatattac
    10141 gatgtcaaaa tcccaagttt tctgagaatt aatctatttc tacatctttc tggaatgatt
    10201 gaacgaatta tgattggtga tggaataaca aatgaacatt tgaaagaggg agcgcaacta
    10261 tttgatactt ttttaaatgt atcaaaagat tttttttcag aaatcaaaga tatttactgt
    10321 attgaaatac ccaaaggaga gtatgagtta atctatacaa tttttgagca gactattttc
    10381 taaatagcat actagcgtct aaaaaaatga aagggtgtcc aaagtatatt tagacgctca
    10441 tttaattaat atttactgta ttttcccgct ttattggata atgttagttg gcacagtaat
    10501 tgcttatata aaagtataag aagaaaggag agaacaaaat ggcgttttgg caaacatttt
    10561 tagttgtgtt gtatggattt ttcattaact gggataagga agggcccaaa atcggaacct
    10621 cccaacctgt cgttgcaggt ttcttgaccg gacttattat gggagatatt caaactggac
    10681 tttatatcgg tgggactttg caattaatga cactaggtat tactagtttt ggtggtgcat
    10741 cagtaccgga ttatcaaaca gcagcattga tcggtactta tttagcaatc tcaactaacc
    10801 aggatgcttc tataggaata actttagcaa taccggtagc aatgttaatt gtacaactag
    10861 atgttctaaa gtggtcaaca aacatttttt ttcaacaaaa ggcagaaaaa ttcgcacaaa
    10921 aagaagatta tcaaaaatta gagtggatgc aatatttggc atcaattaat acaagctttg
    10981 tatctggaat tcctgttttg ttaactgtga tattaggccc aacgttagtg ggaagtgcaa
    11041 tcaattatat tcctgaatgg gttaccggtg gtttaaaggt agcaggggga ctattacctg
    11101 ctgttgggat aggaatgcta cttaaatatt tacctacaaa agattacttt gcctatttgt
    11161 tagttggttt cgttttggca gtttatttcc aaataccaat tttagggatt tcgttacttg
    11221 gcgctgcaat cgcactaatc gtatataaaa atagatcaaa taatactcaa gtagcaccag
    11281 ctgcaacagg aggttttgac gaagatgaat aagaaactga caaaaatcga caaacaagta
    11341 attttccggt ggattctcaa tggtgcaact tcattaaatt atgaaaaaat ggaagggttg
    11401 ggttatgcgt attctatgct tccttttatc aaggacacat ataaagatga tcctgaagga
    11461 atgaaacaat ccgttttagc tcacctacaa ttttttaaca ctacgccgta tacagcgcct
    11521 tacattattg gtatgaatgt tggaattgaa aaagaagaaa aaaataagtc tttggaagca
    11581 gtaagtgctc tgaaaacagg tctaatgggt cctttagcgg gaattggaga tagtgtattt
    11641 gtagttattc catggactat ttttggagca attgcagcta atatggcatt ggaaggtagt
    11701 tttgttggaa ttattttgtg gatactggca tcaattttat taaggattac tgcttatcct
    11761 ttataccttg ctgggaaaga atcgggtgcg agcctactaa ctcaaattga aacaaaatta
    11821 aagtctatta ctgatgcagt atcaatttta gggttaatgg tggtcggtgc actcattgcc
    11881 acagttgtaa aagtgaatgt tggctttgaa tttaaacaag gcgacttaac aatgaccgga
    11941 aatgaaatct ttgatcaagt tatgcctggt ttattacctg catgtgtagt agcaattgtg
    12001 tattatttgt taggacgaaa ggttaaacca gtttatgtaa ttctattaat cttagtagtt
    12061 tctattgcgt taagagcgtt aaatattttg aagtaggtgt tgaatatgga tgaaaaaatg
    12121 aaaggtatta tacatgttcg tattgatgat cgaatgattc atggacaagt agcaacacaa
    12181 tggacaaatc aattgagtgc taacagaatt atggttatta ataatgaagt agccaatgat
    12241 gacgtaaaaa aagctgtagt tcgcctcgct gcaccaccta atgttagaac ttcaataatt
    12301 acaagggaag tagcagttaa aaatattctt tctggtaaat atgaaggaca aaaagttctt
    12361 attgttgcag tttctccaat cgatgttaaa tttttgattg ataacggttt accaataaca
    12421 agtgtaaacg taggaaattt atcaagaagg aatggaacag aacgcatccg tccgacgata
    12481 aatattactg aagaagagag agaggctttt aaagaactaa ttagtgatgg agttgaagtg
    12541 acagtgatac aaactcctag agattcaaaa gaatttttaa aggattttat ttattaagaa
    12601 cagaggatga gtggatggag tttataatta catctcatgg aaatattgcc attggatttg
    12661 tagatacatt aaaaatgttt tttggcgata atttaaattg tcatgtggta acacttggag
    12721 atgcaggcat tgaagaattc cgtgaaaaca ttgaaaaaat agtttctcaa gtaaaaaatc
    12781 aagaagttat ggtatttgct gacttgattg gtggaacacc tttcaacgaa tttgctaaaa
    12841 gaagtgaagt atttcaaaat gggtttcaac ttcttggtgg catgaattta ggaattttga
    12901 ttgaggctgt caatttgaga ttacaaggaa aaaaaagcga tgatatcatc gaaaatctaa
    12961 gaaatatgaa tacaattgcg tgtcttgcag atatgcaaag acatactaac gaagaggatg
    13021 agtgataaat gcaaaaaatt ttttacaagc ctaaagattt ttggtttggg gattgcatgc
    13081 ctttttataa agatggaact ttttatctat ttcatcaacg agatacgaga aatcctaaac
    13141 ctttaactga tccttttggg tggtcactag ttacaaccac tgattttgtg aaatttgaag
    13201 aatacgggga agtaattcat cgtggtggca acgatgacat tgatcaatac atttacgctg
    13261 gaagtattgt ccaaggaatc aatgagtatt tagctttgta tactggacat aatcgcaaag
    13321 ctaagttgga tggaaaaact tcagaagtgt taatgattgc aagttctaaa gatttaattc
    13381 attggaaaaa ggatggcgaa gcagcaaaac ttgttcctca agaaggatat gataagaatg
    13441 attggcgcga tccttttgca atttttgatc aagatcatgg aaaatacgtt ttgatactag
    13501 gagctagaaa accagcaagt aagaaaaagc cgacggggag attggtctat tttgaatccg
    13561 aagatttaaa agattggaat ttcaaaggag actactgggc taataatgaa tttaacatga
    13621 ttgaaatgcc tgatatattt gaaattgatg gaacgtggta tttacttttt tcagagtatt
    13681 gtgaagataa aaagactaaa tatcgagtcg gagcaggatt attttcagat tggaaatcac
    13741 cagtagacga agctttcgac ggaaaagcct attatgcagc aagaacagtc ggaactaatg
    13801 aaactggccg ttttctaact ggctgggtgg caacaaaaga aggggagaac gatcaaaata
    13861 actgggattg gggcggtgca cttgttccac atcaaattat tcaaaagaca gacaaaactc
    13921 ttggtgttaa actgcctgaa aaaataattg agaattttaa agaaatcact ccattttcaa
    13981 gttttaagtt agagaaagaa tttggaaaaa aagaaagagt aattgttcct aaaatcgaag
    14041 attattttct atttgaaaca actttgacta ttacatcgtt aacaaaggag tttgctttaa
    14101 aattatttgt agatagtgat actcaagaag gatacgaatt tcgtatttca ttgattaata
    14161 atcagcttac tttcggaaaa actccgaact atccttggcc tcaaatgttt gataaggggc
    14221 tgcaacgacc actatttttg gaaataggca aagaatatga actaaagtta attgttgatg
    14281 atacgatagc tgttttatat attgataatg ttgctttaag tactagaatg tatgataaag
    14341 tcggaacagc attaagtatg actgttacgg agggagcaat caaggtagat aaactagact
    14401 attcaataaa ttatgaatgt ggattttaaa acaattgaag ggatgatgta agtgaaatta
    14461 aatggtttac aacatattgg aattccaaca aaagattttg gagcttcaaa aatcttttat
    14521 gaatccttag ggtttagtct gattaatgaa gaaatgaatg tgggaagtaa agtcggcttt
    14581 ttcgagttga atggaacgat aatagaaata tgggaagata aaactactga gtgtagagga
    14641 gccattgatc atctagcatt agatacggat gacatagatg aggtattcaa ttcaattcaa
    14701 gatttaggat atgaattatt agataaaaat attatggaat taccgttctg ggaaaatgga
    14761 atccgttact ttaatttcta cggaccaaat catgagatta tagagataag ccaaatgaag
    14821 aaataaatag ataacgaagt gctctaagtt ggtatgaacg aaaagtactt cattttttgg
    14881 tatcagcatt tcaatagttt aatgagaatt tactatttca aattaattca aagtgacagt
    14941 attttttact tatttggttt caaattttgt aatccttcct atttattgga atacactcca
    15001 cattttgtat acaagtcata tcatgtttta gcaaagctct taccaagcaa agttataatg
    15061 atgttttcca aagtttgatc tttcgatgga ggctaacaca tattttttgc aggacagcca
    15121 aaattagatg tatagaatag agtctgtgag gcgtatagac agtttattgt ctgtatgcct
    15181 ttttttattt tgaaaaattt ttagtatttt tcgaaaaatg ctcactccta
//
LOCUS       DAZKKW010000062        15109 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.063, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000062 DAZKKW010000000
VERSION     DAZKKW010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15109
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.063"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            21..605
                     /locus_tag="ACG8PO_001905"
     CDS             21..605
                     /locus_tag="ACG8PO_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0207212.1"
                     /translation="MGLKQSVYIWLYPDNPMSIMADIFVGSILLCLLYLIFFHNLIGY
                     MILDWKGMFDIERKPFNNKQTYTTFAGGDLEEKEVDVTHEIGLALSGIQDADVLGSMI
                     TDTGYNAYEVFYLMYFIWRDSQDIINGRAEKTIISNTEISFCMGEGEMIVYKDGSANI
                     KTAFGDIETSIKELREYVHNPYNFQYLAGRINLV"
     gene            616..867
                     /locus_tag="ACG8PO_001906"
     CDS             616..867
                     /locus_tag="ACG8PO_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0207213.1"
                     /translation="MKKIIDQPTNRLIVEGVIFVTMYFTVGNGISNTIIQKDPQYLLF
                     SLYAWIVQMLWVSLLMLLFYWIVRVILLVLNKAKKKKDR"
     gene            874..1596
                     /locus_tag="ACG8PO_001907"
     CDS             874..1596
                     /locus_tag="ACG8PO_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0207214.1"
                     /translation="MKATKLKLVLFSTAVLLTLSACGTSKSADNTKDTKSSSTVVTES
                     SSAQTSTTMSSMTENNAEKFQKYKDVNQGKKPEEVYNEFISSVKNDESYGNDPLQNKN
                     LTIVLYKPDCPDCQKAESKIMQEIQEGFSYAPTLMHDITIGKPAYEYHAVAVDITDGV
                     PEWVQNIPGLADENNQYHTPTLAVVTPKKIDGTLYWDLMGMTTNTSDEMIESTSNYYM
                     DLGAYKSYQQSEPVMDDQSTIK"
     gene            1726..2469
                     /locus_tag="ACG8PO_001908"
     CDS             1726..2469
                     /locus_tag="ACG8PO_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0207215.1"
                     /translation="MKEISASEAHISQKNQVGIYKVLHGEGIYKIQLLTGYNNGANGK
                     WLKATSIRELRSEVEKIRLGLTSEEIAKLKGNNSFVIEKEKEKHLLYRELKIEEYYEI
                     DLPEKLRIIDQRSEEKWKTEIYPSLNDYKTSGKPMYNYTGFLTYTQYKYAFRKLKGIK
                     QAEIALYEMKKVDRLKLIQELVQNTWHQAIVELEKENGKILTNKYLIVNEIEELIGLF
                     SCEKGEVFLKSSPTYSILDSPYQFIQARR"
     gene            2475..2840
                     /locus_tag="ACG8PO_001909"
     CDS             2475..2840
                     /locus_tag="ACG8PO_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0207216.1"
                     /translation="MSSLKDDYIQAKQEYEKIQMLKSEKVELFLTQKLAEDHIANLDA
                     KTLEEYDRQFNQIHHNIILLVEQAKRRFEKAEYDLLDQGIVCLDKGLRRYYGAELKKQ
                     NVDRNALKHEILELIMQRI"
     gene            2856..3386
                     /locus_tag="ACG8PO_001910"
     CDS             2856..3386
                     /locus_tag="ACG8PO_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0207217.1"
                     /translation="MSQNISAVKQLIEEDLETTVQRLAERIHFANYQTISNWSSRNRN
                     PEEIPMKFFYRIGKEYGVDPIKIFEKTMIYYYHGTPNEVLEHLLEEAKEKSSILELSQ
                     NDKVIQVKCVDPTGKIIVGFDIERKSKIDFITQLKKINNLDILTLRPDHEQLFMSFKK
                     QLKEIQKRYNKVFPNA"
     gene            3548..3913
                     /locus_tag="ACG8PO_001911"
     CDS             3548..3913
                     /locus_tag="ACG8PO_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0207218.1"
                     /translation="MWNNRLKTGLLLIVISCAMMIGMRIQREQSYFEVSANNVIEKCY
                     YGQHYWSEEVRENIDREYVQRIVWDAYSIKDYPKSLTSRLFYSEKDNQKLSDLMMKKV
                     RKLAQSYSEEKAGVIKDKE"
     gene            3930..4319
                     /locus_tag="ACG8PO_001912"
     CDS             3930..4319
                     /locus_tag="ACG8PO_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0207219.1"
                     /translation="MNEETELFLKKVNFLVEKYFLTKHKIMYLIKGLIGGIILWILTF
                     FSLFGGFTNYKKLVDENVKQISASESLRHQAEDLVSNPLFIGGMVEVIIISFVIGYLG
                     MCIFQIIFDLIRGKVFQEFKSEKINLR"
     gene            4349..4573
                     /locus_tag="ACG8PO_001913"
     CDS             4349..4573
                     /locus_tag="ACG8PO_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0207220.1"
                     /translation="MEQTNHITEETRKFICLESFYSEGRYCNKGETYTAYPIEGGFKL
                     VFENGDMNFTTELFECVLKTWSDVLLEVTK"
     gene            4626..4826
                     /locus_tag="ACG8PO_001914"
     CDS             4626..4826
                     /locus_tag="ACG8PO_001914"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0207221.1"
                     /translation="MAYENLQLVVAGVSGDIYLAKAKDGIMDTNYRRVATNDVLVATT
                     EWFIRNNKKLIQFEADENGRHS"
     gene            4888..5130
                     /locus_tag="ACG8PO_001915"
     CDS             4888..5130
                     /locus_tag="ACG8PO_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0207222.1"
                     /translation="MVDKEIKGEIRLFQRENDDWGTEISKNLAEDIELRLSFISMIIE
                     SFAFELGIPQEKLAEILIKASNNSPQHFHIDLGTVN"
     gene            5252..5500
                     /locus_tag="ACG8PO_001916"
     CDS             5252..5500
                     /locus_tag="ACG8PO_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0207223.1"
                     /translation="MSENHLKRLSKGTASMFDANLLIFKSLLEFEKQRVEDIAKYGNY
                     ATVPSVYNDLMKWFKVQTGLEYSQQNLVVKIEEYENHG"
     gene            5527..5691
                     /locus_tag="ACG8PO_001917"
     CDS             5527..5691
                     /locus_tag="ACG8PO_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0207224.1"
                     /translation="MLLESDDPIAELDQLAERAKELEMISGAYRIISEELGQAVVSQA
                     LENYLKKQVD"
     gene            5775..5987
                     /locus_tag="ACG8PO_001918"
     CDS             5775..5987
                     /locus_tag="ACG8PO_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0207225.1"
                     /translation="MPVKTNTEWLDICDELFDTKDKKELAMRVAYAIVQKDKYFAVPS
                     TDYDIEVIVDPITGDKKLRVVIECNA"
     gene            6096..6272
                     /locus_tag="ACG8PO_001919"
     CDS             6096..6272
                     /locus_tag="ACG8PO_001919"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0207226.1"
                     /translation="MDIFEVKPDSGKFIVLQEKRVIAIERTEIEAYKKIHELAEQYIE
                     DLNNNFQEFLTQNE"
     gene            6317..6604
                     /locus_tag="ACG8PO_001920"
     CDS             6317..6604
                     /locus_tag="ACG8PO_001920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0207227.1"
                     /translation="MKIFEIDDPYFALIVAENEEKCMDIYEEVICDVEGKEEFLPCIK
                     ELETTVAITKVANAVSNETNELVGIHEAGNLVFSCLNSNKSALLALDGDLF"
     gene            6976..9216
                     /locus_tag="ACG8PO_001921"
     CDS             6976..9216
                     /locus_tag="ACG8PO_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0207228.1"
                     /translation="MSEFEFLDKSIGSAFLIDPDLFGAWAQSSGYNWAFSDDMVKLLS
                     QTMNWEAYKIKQFLDVVAQPIYTSADDHWSGRELTTLKELAEYNKRYKIPFQVSYMSD
                     DFSVIYSIGKESRYLIPNRHLEIKMNSDYTDYTLSELKALRQSDSEEFSALKNEVSAD
                     NFRRSDIEELKKKQKQQLEKQKKLIEDIEYKRVEELAIQEAKIEKMRQELYKKQQSML
                     AEANKMRFELEKKVEDLNTKIFYLETEINAIEGYFGESTTFVQLRSGQKSLPEEPVVM
                     FQKVRYLDEELGRYFSMREVEGTADTMLTFEQALSSRDGLFDLFSPNKKSISMIRISK
                     SGYGYTNGQMESTNMLEKYKKYHGETLAILLRNGDNLWICWLDENKISFKDDLFYQNK
                     VTEQASETKNMVKEIDWTHTRNLEERLKDAKKQKKAVRGEFASRYILFSILQGVLDNG
                     TMMTLPEKVNVIQAINQKSPYFIASVADGWLEDTRLPSFSELIDKYSFYKETGEDRVL
                     EAEYELNPDKFENMRDYPHKQNDDIYVLYRLRGWSPTSANYTYYNSDNRSNSESGRDL
                     AYSIDLKDGIYQINLVKQGKFQKQMFSRTFVGVDSTTGENVYDYYYERETEYYIRGQK
                     AYNKSDYWGDKAKRLTANFRVYESEFINLTFLNSVFIDYVITTKRIGSYSSTYASLVP
                     MLNSMSRFLKEREEKELEYIKEIMPDFEMHEWQIALSDWKLTNKVRRITPFQAKRFLK
                     AMKFVQ"
     gene            9354..10151
                     /locus_tag="ACG8PO_001922"
     CDS             9354..10151
                     /locus_tag="ACG8PO_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0207229.1"
                     /translation="MSEDLRELKLRKKQYDQYFSGLLNKHIEEGLDHDFGKDEYCLYC
                     ERYNEKMSEYLQLKKEIQKLEKKVHAWKYKDSMSGYGESQNNPRKKKQAYVLKMVNGE
                     ELIVLGYSKEEVRELVETPVRKKVETINEIDDSQYNSIYIETSHGGYKKLATFLRSSK
                     VGIISKKDISAVTDSFCTLSVPKAYFQYNSNRFDRLEKFCRPYTVLKTKKFNKTKRVS
                     IKLPTGQISLGEKDYLIKVGKELIIKVPERLFKENFEIRETLVWKEE"
     gene            10191..10373
                     /locus_tag="ACG8PO_001923"
     CDS             10191..10373
                     /locus_tag="ACG8PO_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0207230.1"
                     /translation="MAVNRKLQVLITDDFGGAEWFDVQSIDFEEEVVVIENVYGEEYS
                     YSLEGENVEDLRLIGE"
     gene            10417..10704
                     /locus_tag="ACG8PO_001924"
     CDS             10417..10704
                     /locus_tag="ACG8PO_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0207231.1"
                     /translation="MINKKTGKRIFSSKDEVIRDLSENFEPIELFFHQLNLEIEKLLK
                     EPTEGRINKVLKLIACKDSHAYMLYPKWYADKNIAEINDYLASIKERNAIN"
     gene            10741..11028
                     /locus_tag="ACG8PO_001925"
     CDS             10741..11028
                     /locus_tag="ACG8PO_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0207232.1"
                     /translation="MAEVKFGSKEWFEELNSMIQFPEKFTFYYNKRDNIIQVALLETK
                     GRLENTRWSMIKPSALRFHPKDLTAVVAVKEVQDSQEYYEVVKNLKDIWCK"
     gene            11119..11358
                     /locus_tag="ACG8PO_001926"
     CDS             11119..11358
                     /locus_tag="ACG8PO_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0207233.1"
                     /translation="MKELEGIKKRNQERLELLNENRNRMFSYYESMRCLNKSRSEIIE
                     SSKYKQLMEERVSLIQEIETESIFPDHFPKVRFYN"
     gene            11486..11815
                     /locus_tag="ACG8PO_001927"
     CDS             11486..11815
                     /locus_tag="ACG8PO_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0207234.1"
                     /translation="MKLEGSLQNRMMEGRTTNKEIVVGMGATELLYTDRNPYTVIEVK
                     SKNRILVQADGAINKATFPDQEWEYSRNPEGQILELIKTKNGWKVLKEDTYFYIGDRE
                     KYYDPSL"
     gene            11868..12698
                     /locus_tag="ACG8PO_001928"
     CDS             11868..12698
                     /locus_tag="ACG8PO_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0207235.1"
                     /translation="MFSQEEIISKSQPDKNGTITMKKKIPCYRCSGSGQYKFMSMGKI
                     TYGTCFKCDGDGFLVVTEKSFTPEYRTKLNDLNKKRADRKIRIAREKESQKNFDRYGF
                     EKGYIYVVIEKNSYAIKDELHHQGATYDPLLGWFFKEKQEQYKTQKVFVEEFSVINDE
                     GIIELKLEEYSKRQQEILRAKHREEGEFIGTIAQRLELDLKLTNTVTIDTQWGNVYLH
                     LFEDQNHNSLIWKTNKFLYYDEWDVGEIRKVKATIKAHDYYRDTKQTWIKNVKIQKNE
                     "
     gene            12713..13030
                     /locus_tag="ACG8PO_001929"
     CDS             12713..13030
                     /locus_tag="ACG8PO_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0207236.1"
                     /translation="MKREEYKQRLNELLEEDETLTHGSPDEILYMIDNMVIFGGYELG
                     NRSVDHNILEFDDVSWEEILDWGILAVPETKTYISDTMVPFFEELDYKRLPKNENHIL
                     GGN"
     gene            13033..13266
                     /locus_tag="ACG8PO_001930"
     CDS             13033..13266
                     /locus_tag="ACG8PO_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0207237.1"
                     /translation="MLSDLLILLIYLGVTEIIAYFLLVIGDYGISSAITQRFRLPVSE
                     LKKMMRLSAYLLMFLNVVTLIFVVIGVTFNLME"
     gene            13516..14442
                     /locus_tag="ACG8PO_001931"
     CDS             13516..14442
                     /locus_tag="ACG8PO_001931"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0207238.1"
                     /translation="MAFSTKHYYPKPIILTVKTGISLIGIKLTTNLDLKPSKFNARTA
                     LLLDEPFQYSTIFYYENDPEFTEKLYRFIDKLKALRKVYIDEYYRSLLTSYEADIKAI
                     NTALEKLEITILTGVSSSKRQRSSEDIATPEELHMWIKKTFKQRKKKIQSLYPKKEYF
                     EFYSFLNVCNDFLKSLGEQSLKFTNSLNLFFEIGSKIDWFLLNITDCSYHQSPVFKKL
                     FRSHITLNNDLVSVVGAFSILKQPRNIELYLAQEQSFIEKVDQYKKIFHELLITGNRI
                     IDLETMNIKAEKDKESLLKEIIKSKETERKVA"
     gene            14654..14815
                     /locus_tag="ACG8PO_001932"
     CDS             14654..14815
                     /locus_tag="ACG8PO_001932"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0207239.1"
                     /translation="MNYYEIWWKVKSSGQILNTTLTASSREDAKSKVLAKYGYKNWEI
                     RFAKAEICS"
BASE COUNT         5852 a         1879 c         2968 g         4410 t
ORIGIN      
        1 atggaatgag atgatgcaaa atgggcttga aacaatcagt ttacatctgg ttatatcctg
       61 ataatcccat gtcaataatg gctgatattt ttgtaggtag cattttattg tgtttactgt
      121 atctaatttt cttccacaat ttgatcgggt acatgatcct tgattggaaa ggaatgttcg
      181 atatagaaag aaaaccattt aataataagc aaacttatac cacctttgca ggtggagatt
      241 tggaagaaaa agaagttgat gtgacacatg aaattggatt agctttatca ggaattcaag
      301 atgctgatgt attaggatca atgataacgg ataccggata taacgcctat gaagtatttt
      361 atctcatgta ttttatatgg cgagatagtc aagatattat caacggtcgt gctgaaaaaa
      421 caatcatttc aaatactgaa atttctttct gtatgggtga aggggaaatg attgtatata
      481 aagatggatc agcaaacatt aaaaccgcat ttggtgatat tgaaacaagt attaaagagc
      541 ttagggagta tgtacataat ccctataatt ttcaatactt agcaggaaga ataaatttag
      601 tttaggagta agaatatgaa aaaaattatt gatcagccaa caaatcgctt aatagtcgaa
      661 ggcgttattt tcgtaacaat gtatttcact gtgggaaatg gtatttcaaa tacaatcatt
      721 caaaaagacc cacaatactt gttatttagt ctttatgctt ggattgtgca gatgctttgg
      781 gtgtctttat taatgctgtt gttttattgg atagtacggg ttattctttt agtgttaaac
      841 aaagcaaaaa agaaaaagga taggtgagta aaaatgaaag caacaaaatt aaaacttgta
      901 ctattttcaa cagcagtatt actaacttta tcagcatgtg gtacatcaaa gagcgctgat
      961 aataccaaag acacaaaatc tagtagtaca gtggtgactg aaagcagttc agcgcagaca
     1021 tctacaacta tgtcttcaat gactgagaac aatgctgaaa aatttcaaaa gtataaggac
     1081 gtaaatcaag gtaaaaagcc ggaagaagtt tataacgagt ttatctcttc agtaaaaaat
     1141 gatgaaagtt atgggaatga tccattacaa aataaaaatc ttactattgt tctttacaaa
     1201 cctgattgtc ctgattgcca aaaagcagaa tccaaaatta tgcaagaaat acaagaagga
     1261 tttagttatg ctccaacatt aatgcacgat attaccatcg gcaaacctgc ttacgaatat
     1321 catgcagttg cggtagatat aacggatggc gtacctgaat gggtgcaaaa tatccctggt
     1381 ctggctgatg agaacaacca atatcacacg ccaacactag cagtagtaac accaaagaaa
     1441 attgatggaa cactatactg ggatttaatg ggaatgacaa caaatacatc cgatgaaatg
     1501 attgaaagca ccagtaacta ctatatggat ttgggagcct ataaatcata ccaacagtct
     1561 gaacctgtta tggatgatca atctactatt aagtagtttt tagggcgtaa gccctatttt
     1621 tttaaaatta tgcgataaca aattaatgat ttctgaaaca aacgttctta ctcaactact
     1681 cgaaacacac aagctaatta actagattat aaaggagaat gcgtaatgaa agaaatcagt
     1741 gcaagtgaag cccatatttc tcaaaagaat caggttggta tatataaggt gcttcatggt
     1801 gaagggattt ataaaataca gttacttact ggttacaata acggtgcaaa tgggaagtgg
     1861 ttaaaggcaa caagtattcg tgaacttaga agtgaagtag aaaaaattag gcttggtctc
     1921 acaagtgaag aaatagctaa actgaaggga aataattctt ttgtaattga aaaagaaaag
     1981 gaaaaacatc tactttatcg tgagctgaaa attgaagaat actatgaaat tgatttacct
     2041 gaaaaattaa gaattattga tcaacgttca gaagaaaaat ggaaaactga aatatatcca
     2101 agcttaaatg actacaagac atcaggaaag cccatgtata actacacagg ctttttaacc
     2161 tacacacaat acaaatatgc ctttcgcaaa ttaaaaggca ttaaacaagc tgaaattgcc
     2221 ctgtatgaga tgaaaaaagt ggatagattg aaattgattc aagagttagt gcaaaataca
     2281 tggcatcaag cgattgtcga gctagaaaaa gaaaatggga aaattttaac taataaatat
     2341 ctgatcgtaa atgaaattga agaattaatt ggtttatttt catgtgaaaa aggtgaagtt
     2401 ttcttgaagt catcaccaac ctattcaatt ttggacagtc cgtatcagtt tatacaggca
     2461 aggagatgag ttagatgtct agcttgaaag atgactatat ccaagcaaaa caagagtatg
     2521 aaaaaataca gatgttgaaa tctgagaaag tcgaattatt tttaacacaa aaattagcag
     2581 aggatcatat tgctaactta gatgctaaaa ctttagagga atatgatcgg cagtttaatc
     2641 agatacatca caatatcatt ttgcttgttg agcaggcaaa gcgaaggttt gaaaaagcgg
     2701 aatatgatct attagatcaa ggaattgtat gcttggataa gggattgcga agatattatg
     2761 gtgcagagtt aaaaaagcaa aatgtagata gaaatgcact aaaacatgaa atcttagaat
     2821 tgataatgca acgaatttga agggatagaa agcaaatgtc acaaaacatt agtgctgtaa
     2881 aacaattaat agaagaagat ttagagacca cagttcagag attagcggaa cgaatccatt
     2941 ttgcaaatta tcaaaccatc tctaactggt caagcaggaa cagaaatccg gaagaaattc
     3001 caatgaaatt tttttatcga atcggaaaag aatatggtgt tgatccgata aaaattttcg
     3061 agaagaccat gatttattac tatcatggaa cacctaacga agtgttagaa catcttttgg
     3121 aagaggcaaa agaaaaatca tcaatattag agctatcgca aaatgacaaa gttattcaag
     3181 tgaaatgcgt tgatccaaca ggaaagatta ttgtcggttt tgatattgaa agaaaatcca
     3241 aaatagattt tattactcaa ttaaaaaaga taaataattt agatattcta acgcttagac
     3301 cagatcatga acagttgttt atgagtttta aaaaacaatt gaaagaaata caaaaaagat
     3361 ataacaaggt gtttcctaat gcgtgaaaag ctcaaagaaa tcagagtttt ttggataatt
     3421 aacaaagaat ttatcatctt atatactctt ggatttctca gcatgatctt gttttggtgg
     3481 ttatatttaa aaaagtaatt ggagagggtc aagaggattt ttatcaagat aaggatgtgg
     3541 aaactgcatg tggaataata gattaaaaac aggattgtta ttaatagtta tttcctgcgc
     3601 tatgatgatt ggtatgagga tacaaaggga acaatcctat tttgaagttt ctgctaacaa
     3661 cgtaatagaa aaatgctatt acggtcaaca ttattggagt gaggaagtca gagaaaatat
     3721 agatcgtgaa tatgttcaaa gaattgtttg ggatgcatat tctatcaaag attatcctaa
     3781 aagtttaacg agtagattat tttacagcga aaaagataat caaaaactct cagatttgat
     3841 gatgaaaaaa gtaaggaaac ttgcacaaag ctattcggaa gaaaaagcag gagtaataaa
     3901 agataaggaa taaatggaga aatttgctat tgaatgaaga aacggaatta tttttaaaaa
     3961 aagtgaactt tcttgtagaa aaatattttt taacaaagca caaaattatg tatctaataa
     4021 aaggattgat cggtggaatt attttatgga tacttacttt cttttcttta tttggtggat
     4081 ttactaatta taaaaagtta gtagacgaaa atgtgaagca aattagtgca tctgaatcat
     4141 taagacatca agctgaagat ttagtaagta atccattatt tataggaggt atggtagaag
     4201 tgataataat tagcttcgtt attggttatt taggtatgtg tatcttccag ataatctttg
     4261 atctaataag aggaaaagtc tttcaagaat ttaaatctga aaaaataaat ttacgttaga
     4321 gataataggc tatgagagga gaagaatagt ggaacaaact aatcatatta ctgaagaaac
     4381 taggaaattc atttgtttag aatcgtttta ttcggaaggt aggtattgta acaaaggtga
     4441 aacttatacg gcatatccta ttgaaggggg atttaaatta gtttttgaaa acggagacat
     4501 gaattttacc actgagttgt ttgagtgtgt tcttaaaacg tggtcagatg tgttgcttga
     4561 agtcactaaa taatcaatgt tatctagtag aattaagcaa ctaaaaaaga aagaaggaag
     4621 aaaatatggc gtatgaaaat ttacaattag tagtagcagg agttagtggc gatatatatc
     4681 ttgcaaaagc aaaagatgga attatggaca cgaactatag aagagttgct acaaatgacg
     4741 ttttagtagc aacgacagaa tggttcatta gaaataacaa aaagttaatc caatttgaag
     4801 cagatgaaaa tggtagacat agctagtttt atacaaacga tcaaaaaaaa gctgatcgga
     4861 tcatagcgat tctaaaggag aatgatgatg gtggacaaag agattaaggg cgaaataaga
     4921 ttatttcaaa gagaaaatga cgattggggt acggaaatat caaagaatct tgctgaagat
     4981 atagagttac ggttgagctt tataagtatg ataattgaaa gttttgcttt tgagcttggt
     5041 ataccgcaag aaaagttagc agaaatactg attaaagcaa gtaacaatag tcctcagcac
     5101 tttcacattg atctaggtac agtaaattag tctttaatca ttaatattaa tttttttgat
     5161 aagctgatct gattaatatt tcttaatgta atcattccaa tattgatctt cagccgttaa
     5221 cggaatatat agaggaaagc ggtgaaatga aatgtctgaa aatcatttga aacgattatc
     5281 gaaaggaacg gcaagtatgt ttgatgccaa tctactaatc tttaaaagcc tgctagaatt
     5341 tgaaaaacaa cgtgtagaag atattgctaa gtatggtaac tatgctactg ttccgagtgt
     5401 ttacaatgat ttaatgaaat ggttcaaggt acaaacaggg ttggaatata gccaacagaa
     5461 tttagtggta aaaatagagg agtatgaaaa tcatggctaa aagtttatca caagaattaa
     5521 gagaatttgc ttttagagag tgatgatcct atagctgaac tagatcaatt agcggaaaga
     5581 gcaaaagaat tagaaatgat ttctggtgcg tatagaatta tttctgaaga attaggtcaa
     5641 gcggttgtgt cacaagcact tgaaaattat ttgaaaaaac aagttgatta gtgacagaaa
     5701 taacggaata ggatataaat gatggattca agatgatatt agttaataaa aaatagtatg
     5761 gagggcaact aattatgcca gtaaaaacaa acactgaatg gttagatata tgtgatgaac
     5821 tatttgatac taaagacaag aaagaactgg cgatgcgtgt agcatatgct atagttcaaa
     5881 aagataaata ttttgctgtt ccatctacag actatgacat tgaagtgata gttgacccta
     5941 ttactggaga taagaaatta cgagtagtta ttgagtgtaa cgcctaatgt attctttaga
     6001 acaataggag gaatcttatt tcttaaaagt gcaggaatca tatagaagag tggttgctaa
     6061 ccgtcttaag tatattagaa agagatgcaa atttaatgga tatttttgaa gttaaacctg
     6121 attcaggaaa atttattgtt ttgcaggaaa aaagagtgat tgcaattgaa agaacagaaa
     6181 tagaagcata taaaaagata catgaactag cagaacaata tattgaagat ttgaacaata
     6241 attttcaaga atttctaacg caaaatgaat aggcaggtat agacagagat aacaaactag
     6301 gaaaggaaga ccataaatga aaatttttga aattgatgac ccgtattttg cacttatagt
     6361 agctgaaaac gaagaaaaat gtatggatat atatgaagaa gtaatttgtg atgtagaagg
     6421 caaagaagaa tttttacctt gtataaaaga acttgaaacg acagttgcga ttacaaaggt
     6481 tgcaaatgca gtgagtaatg aaacaaacga gctagtagga attcatgaag ctggtaatct
     6541 tgtgtttagt tgtcttaata gcaataaatc tgcattattg gctcttgatg gggatttatt
     6601 ttaatccaac aaccaccaaa atcacctaaa atggtcgatt tgaccagttt ggataaataa
     6661 ttgtgtagat caattttgta aatgaagatg ttagataaac tagacatcaa tctgtatagc
     6721 taaagaaaag agaacggtaa tttatatccg ctctcttttc tttagctatt tatagaatat
     6781 accttctcta aatccttcca tcaatgtgag cttgcttaat tcatttgaaa ttaaaaaagt
     6841 tattgctatt atacacaccg ttgtgtataa tataaagtgt aaatataatt gttcctttga
     6901 gctattcatt agagttgaag catagaatag tttcatagaa tccatagtaa aaaatttgag
     6961 agtaaaggag aagttatgag cgaatttgaa tttttagata aaagtattgg ttctgctttt
     7021 ttgattgatc ctgatctgtt tggagcatgg gctcaatcat caggttataa ttgggctttt
     7081 agtgatgata tggtcaagct tttaagtcaa acgatgaatt gggaagctta taagatcaag
     7141 caatttttag atgttgttgc acagccgatt tatacatctg ctgatgatca ttggtcaggt
     7201 agagagttga caacactcaa agaactagcg gagtacaata aaagatacaa aattccgttt
     7261 caagtttctt acatgtcaga tgatttttcc gttatttact caataggtaa agaaagcaga
     7321 tatttaatcc ctaataggca cttagagata aagatgaata gcgattatac cgactatacg
     7381 ctaagtgagt taaaagctct tagacagagc gacagtgagg aatttagcgc actcaaaaat
     7441 gaagtgagtg cagataattt tcgcagatca gatattgaag aactgaaaaa aaagcaaaaa
     7501 cagcaactag aaaagcaaaa aaaattaatt gaagacattg aatataaacg agtagaagag
     7561 cttgcaatac aagaagccaa aattgaaaaa atgcgacaag aactctataa aaaacagcag
     7621 tcaatgttag cagaagcaaa taaaatgcgg tttgaattag agaaaaaagt agaagatttg
     7681 aatactaaaa ttttttattt ggaaactgag attaatgcaa tcgaaggcta ttttggtgag
     7741 agtacaactt ttgttcagtt gcgttcaggt caaaagagct tgccggaaga gccagtggtg
     7801 atgtttcaaa aagtacgata tctagatgaa gaattaggaa gatatttcag tatgcgagaa
     7861 gttgaaggta cagctgatac catgctgact tttgagcagg cgctatcctc acgagatggt
     7921 ttgtttgatt tattctcacc gaacaaaaag tcaatctcaa tgatacgtat ttccaaatca
     7981 ggatatggct acactaacgg tcaaatggaa agtacaaaca tgctagaaaa gtataaaaaa
     8041 tatcatggag aaactttagc aattttactg agaaacggtg ataacttatg gatttgttgg
     8101 ttggatgaaa ataagatcag tttcaaagat gatttgtttt accaaaacaa agtaacagag
     8161 caggcatctg aaaccaaaaa tatggtaaag gagatagatt ggacacatac tcgtaattta
     8221 gaagagcgat tgaaagatgc taaaaagcag aagaaggctg ttagaggaga gtttgctagt
     8281 cgttatattc ttttttcaat tttgcaaggt gttctagata atggaacgat gatgacctta
     8341 ccagaaaaag taaatgtgat ccaagcaatt aatcagaaaa gcccatattt tattgcaagc
     8401 gtagcggatg gatggttaga agacacacgc ttgccgtcat ttagcgagtt gattgataaa
     8461 tacagtttct ataaagaaac aggtgaagac agagtattag aagcggaata tgagttgaat
     8521 cccgataaat ttgaaaatat gagagattat ccacacaaac aaaatgatga catttatgtg
     8581 ttataccggt tacgtgggtg gtcgcctaca tcagcaaatt acacttatta caacagtgat
     8641 aaccgttcaa atagtgagag tggacgtgac ttagcatatt cgattgactt gaaagacggc
     8701 atttatcaga tcaatttagt aaaacaggga aaattccaaa aacaaatgtt ttcccgaacg
     8761 tttgttggag ttgattctac tacaggcgaa aatgtctatg attactatta tgaaagagaa
     8821 acagagtact atattcgagg acaaaaagcc tacaacaaaa gtgactattg gggtgacaag
     8881 gcgaagcgat taactgcaaa ttttagagtg tatgaatccg aatttataaa cttaaccttt
     8941 cttaatagtg tttttattga ttatgtcatt actacgaaac gtattggaag ttattcaagt
     9001 acctacgcat ccttagttcc aatgttaaac tcgatgagcc gtttcttaaa agaaagagaa
     9061 gagaaagagc tagagtatat caaagaaatt atgccagatt ttgaaatgca cgaatggcaa
     9121 attgctttat cagattggaa attgacgaat aaggttcgta gaataactcc ttttcaagcc
     9181 aagcgctttt tgaaagctat gaaatttgtt caatagaaaa atccaccaga caagctgatg
     9241 gagactaacc caattactaa cagttgtaat tggtgttagc ttataaatta attatggtgt
     9301 ttagtcaatc ccaaattgtg aaacggcacc aataattgga aaggaaaaat gaaatgtctg
     9361 aagatttacg agagctgaag ttaagaaaga agcagtatga ccagtatttt tccggtttgt
     9421 tgaataaaca tattgaagaa ggacttgatc atgattttgg aaaagatgaa tattgtttgt
     9481 attgcgagcg ttacaatgaa aaaatgtctg aatatctaca gctgaagaaa gagatacaga
     9541 aattagagaa aaaagtacat gcttggaagt ataaagattc catgtccggt tatggtgaat
     9601 ctcaaaataa cccaagaaaa aagaagcaag cgtatgttct aaagatggta aatggggaag
     9661 agttaattgt attagggtac agtaaagaag aggttcgaga actcgttgaa acacctgtca
     9721 ggaaaaaggt agaaacaata aatgagatag acgacagcca atacaatagc atctatattg
     9781 aaacaagtca tggtggatat aagaaattag caactttcct tagaagttct aaagttggga
     9841 taatttctaa aaaagacata agtgctgtta ccgatagttt ttgtacttta tctgttccta
     9901 aagcatattt tcaatacaac agtaatagat ttgatcgctt ggagaaattc tgtagaccat
     9961 atactgtatt gaaaactaaa aaattcaata aaactaaaag agtttcaatc aagttaccga
    10021 ctggtcaaat ctcgctaggc gaaaaagatt atctgataaa agtaggaaaa gagttaataa
    10081 tcaaagtgcc ggaacgctta tttaaagaaa attttgagat acgtgaaacc ttggtatgga
    10141 aagaagaata atagtttgga agacatagca gggtataagg aggaattgaa atggcagtaa
    10201 atagaaaatt gcaggtattg attacagacg actttggcgg agcagagtgg tttgatgtgc
    10261 agtctataga ttttgaagaa gaagtcgtag ttatagaaaa tgtatacggc gaagaatact
    10321 catattcgct tgaaggagag aatgttgagg atttacggtt gattggtgag tagaaaatag
    10381 cagaaatatc aaaatggaga tgaaatcagc gcgtatatga ttaacaagaa aacaggaaaa
    10441 cgtattttca gcagtaaaga cgaggtaatt cgggatttga gtgaaaactt tgaaccaatt
    10501 gaattatttt tccatcagtt aaatctagaa attgaaaaat tactaaagga acctacagaa
    10561 ggacgaatta ataaagtatt gaaattaata gcatgtaaag atagtcatgc ttacatgctt
    10621 tatccaaaat ggtatgcaga caaaaatata gctgaaataa acgattatct agctagtata
    10681 aaggaacgca atgcaattaa ttaacaatag acgaaaaatg aaattaaagg agataggaaa
    10741 atggcagaag ttaaatttgg atcaaaagaa tggtttgaag agttaaactc aatgattcag
    10801 tttccagaaa agttcacttt ttactacaac aaaagagaca atatcattca agtagcttta
    10861 cttgaaacta agggaagatt agaaaatact cgatggagta tgataaaacc atcagcactc
    10921 agatttcatc ctaaagactt gacagcggtt gtagcggtaa aagaggttca agactcacaa
    10981 gaatactatg aagtagtaaa aaatctgaag gatatctggt gtaaataatt ctaaattgac
    11041 cagttatttg gtcaaaaata gtatgtgatt atttcatcca atatggcatc ataattggta
    11101 tgaaagggga taaattattt gaaagaacta gaaggtatta agaaaagaaa tcaggaaaga
    11161 ttggaactgt taaatgaaaa tagaaatagg atgtttagtt actatgaatc catgaggtgt
    11221 ttaaataaat ctagaagtga aattatagaa tcatcaaagt ataaacaatt gatggaagag
    11281 agagtttctc tcattcaaga aatagaaaca gagtctattt ttccagatca ttttcctaaa
    11341 gtccgatttt ataattaaac attaacaaat ggaacgatgc taaataggca tcgtttttta
    11401 tttatttgtt aaatttattt atttacaaaa tgtgtatcag tgtgtataat atatttgtag
    11461 ataagttaag aaaggatgat ctgaaatgaa attagaaggt agcttacaaa acagaatgat
    11521 ggaaggcaga acaacaaata aggagattgt agttggtatg ggagcaaccg agcttctata
    11581 taccgacaga aatccttata cagtgattga agtaaaaagc aaaaacagaa ttttggttca
    11641 agcggatggg gcaatcaata aagctacttt tccagatcaa gagtgggaat acagcagaaa
    11701 tcctgaaggt caaatattgg aattaattaa gacaaaaaat ggatggaaag tgcttaaaga
    11761 agatacttat ttttatatag gtgatagaga aaaatactac gatcctagtt tataaacaaa
    11821 ttgaagatga gcgaggtttt atcctcgctc agaaaggaga atgaaggatg ttcagtcaag
    11881 aagaaatcat cagcaaatcg caaccggata aaaatggaac tataaccatg aagaaaaaaa
    11941 ttccttgcta tcgttgtagc ggatcaggtc agtacaagtt tatgtctatg gggaaaatta
    12001 cttatggcac atgctttaag tgtgatggtg atggatttct tgttgtaact gaaaaatcct
    12061 ttacgccaga atatagaact aagctaaatg atttaaataa aaaaagagct gatcgaaaaa
    12121 taaggatcgc aagagagaaa gaatctcaga aaaattttga tcgttatggc tttgaaaagg
    12181 gttatattta cgtggttata gagaaaaata gctatgcgat caaagatgaa ctgcatcatc
    12241 aaggcgctac gtatgatcct ttattaggtt ggttttttaa ggaaaaacaa gaacaataca
    12301 aaactcaaaa agtttttgta gaagaatttt ccgtaatcaa tgatgaagga attattgagt
    12361 tgaagctaga agaatacagc aaaagacaac aggagatttt aagggcgaag catagagaag
    12421 agggtgagtt tattggtaca atcgctcaac gattggaatt ggatttgaaa cttaccaata
    12481 ccgtcaccat agatacacaa tggggcaatg tttacctgca tttatttgaa gatcaaaacc
    12541 ataattcatt gatttggaag acaaataaat ttctttatta tgatgaatgg gatgttggag
    12601 aaataaggaa agttaaagcg acaataaaag cgcatgacta ctacagagat accaagcaga
    12661 cttggattaa aaatgttaaa attcaaaaaa atgagtaaga aaggataaaa gtatgaaaag
    12721 agaagaatac aaacaaagat taaacgaact gcttgaagag gacgaaacat taactcatgg
    12781 ttcgccggat gaaattctat acatgattga taatatggtg atttttggtg gatatgaact
    12841 tggtaatagg tcagttgatc ataatatttt agaatttgat gatgtatcat gggaagaaat
    12901 tcttgattgg ggaattttgg cagtacctga aactaaaacc tatattagtg acactatggt
    12961 tccttttttt gaggaattgg attataaaag acttcccaaa aatgaaaatc atatactggg
    13021 aggaaactaa caatgttaag tgacttactt attttactga tttatctagg tgtaactgaa
    13081 attattgcgt attttttgct tgtaattggt gattatggta tttccagtgc aatcactcaa
    13141 aggtttagat taccagtaag tgaactaaaa aaaatgatga gattatcagc atatctttta
    13201 atgtttttga atgtggtcac gttaatcttt gtagtgatcg gtgtgacttt caatttaatg
    13261 gaatagaggg aaatctataa tggaaaaaag agaaaagaat gaacatattt tttgagtcaa
    13321 tattgttgat ggttttgcag tcagcgctta ccagaaagct atacctattt ttgatttaaa
    13381 taaccaagtt atcaacttct ttgtaatagc aggaagtatt atcaactctt ttttcattac
    13441 taatatatta agaatagtgt tcaagcgaat ctatcaattt ttcgctaatg aaagataaag
    13501 aaaaggagaa atattatggc gttttcaacg aagcattatt atcctaaacc aataattctc
    13561 actgttaaaa ctggaatcag tttaattggc ataaaactta ccacaaacct tgacttaaaa
    13621 ccaagtaagt ttaatgcaag gactgcctta cttcttgatg aaccctttca atatagcact
    13681 attttttatt atgaaaacga tccggaattt acggaaaaat tatatcggtt tatagataag
    13741 cttaaagcac ttagaaaagt gtatattgat gaatactata ggagtctttt aacttcatat
    13801 gaagcggata taaaagcaat taatacagcc ctagaaaaac ttgaaataac gattttaaca
    13861 ggagtttcaa gttccaaaag acaaagatca agcgaagata ttgcaacacc tgaagagtta
    13921 cacatgtgga tcaagaagac atttaaacaa cgaaagaaaa aaattcaatc cctatatccc
    13981 aaaaaagaat actttgaatt ttactcgttc ttaaatgtat gcaatgattt cttaaaatcg
    14041 ttaggtgaac aatcacttaa atttactaat tcactcaatt tattttttga aattggatca
    14101 aaaattgact ggtttctatt gaatattacg gattgtagct accaccaatc acctgttttt
    14161 aaaaaattgt tccgttccca cataacgctt aacaatgatt tggtaagtgt cgttggtgct
    14221 ttcagtattt taaaacaacc tagaaatatt gaattgtatt tagcacaaga acaatcattt
    14281 atagaaaaag tagatcaata caaaaagatt tttcacgaac tattaattac cggaaataga
    14341 attattgatc ttgaaactat gaatattaaa gcagaaaagg ataaagaatc acttttgaaa
    14401 gaaatcataa agagcaaaga aacagaaaga aaagttgcgt gaaatcaatt ctatgcatga
    14461 gattgcagta gatagaaaga ttcaagatat agattgaccc aacaaagatt taccagaaga
    14521 gaagaaaact catgttaaaa aaacgggaat attggttcga tggcgaaatg ttagtatttg
    14581 ttgaaattga tagtgatctt gaattaagaa ttaactttat gataaatcaa gggttataga
    14641 ggagtgaaaa atagtgaatt attacgaaat ctggtggaaa gtaaaatcga gcggtcaaat
    14701 tttgaacacc acattaacag caagcagtag agaagatgca aaaagcaaag tgttagcaaa
    14761 gtatggatat aaaaattggg aaattagatt tgccaaagct gaaatttgta gttaattgat
    14821 ataaaaatat aagtaaatag ggagagatgt ttggtgggaa ttaaaaaatc agagtgaaat
    14881 ctaacctcac caccaaatca tggataagca tattgctaag tttgtaagaa acgatgagga
    14941 tagaattggt gatatagaaa gttttcataa ggatttgtca aataaaaaac tacccaaaaa
    15001 tgcagattat atgttaggag gaacctaaaa gtggtaagca atttgtttag tattttgata
    15061 cttttagtgg cgttagaatt attattttat tgtgtgcttt tgtttgtaa
//
LOCUS       DAZKKW010000063        15050 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.064, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000063 DAZKKW010000000
VERSION     DAZKKW010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15050
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.064"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(23..301)
                     /locus_tag="ACG8PO_001933"
     CDS             complement(23..301)
                     /locus_tag="ACG8PO_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0207240.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            569..2578
                     /gene="metG"
                     /locus_tag="ACG8PO_001934"
     CDS             569..2578
                     /gene="metG"
                     /locus_tag="ACG8PO_001934"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFQ0207241.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(3057..3644)
                     /locus_tag="ACG8PO_001935"
     CDS             complement(3057..3644)
                     /locus_tag="ACG8PO_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0207242.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            3819..5141
                     /locus_tag="ACG8PO_001936"
     CDS             3819..5141
                     /locus_tag="ACG8PO_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFQ0207243.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            5296..6072
                     /locus_tag="ACG8PO_001937"
     CDS             5296..6072
                     /locus_tag="ACG8PO_001937"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFQ0207244.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            6065..6634
                     /gene="rnmV"
                     /locus_tag="ACG8PO_001938"
     CDS             6065..6634
                     /gene="rnmV"
                     /locus_tag="ACG8PO_001938"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043822 - ribonuclease M5 activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HFQ0207245.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            6627..7511
                     /gene="rsmA"
                     /locus_tag="ACG8PO_001939"
     CDS             6627..7511
                     /gene="rsmA"
                     /locus_tag="ACG8PO_001939"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFQ0207246.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            7962..8591
                     /locus_tag="ACG8PO_001940"
     CDS             7962..8591
                     /locus_tag="ACG8PO_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0207247.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(8715..9824)
                     /locus_tag="ACG8PO_001941"
     CDS             complement(8715..9824)
                     /locus_tag="ACG8PO_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0207248.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            10033..10455
                     /gene="mgsA"
                     /locus_tag="ACG8PO_001942"
     CDS             10033..10455
                     /gene="mgsA"
                     /locus_tag="ACG8PO_001942"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HFQ0207249.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     regulatory      10642..10740
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01831"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="THF riboswitches regulate genes encoding folate
                     transporters and folate metabolism; They are most commonly
                     found in Clostridiales and Lactobacillales; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="tetrahydrofolate"
                     /db_xref="RFAM:RF01831"
     gene            10778..11314
                     /locus_tag="ACG8PO_001943"
     CDS             10778..11314
                     /locus_tag="ACG8PO_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HFQ0207250.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(11354..13117)
                     /locus_tag="ACG8PO_001944"
     CDS             complement(11354..13117)
                     /locus_tag="ACG8PO_001944"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0207251.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(13120..14835)
                     /locus_tag="ACG8PO_001945"
     CDS             complement(13120..14835)
                     /locus_tag="ACG8PO_001945"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0207252.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
BASE COUNT         4826 a         2733 c         3050 g         4441 t
ORIGIN      
        1 cttgaaaaat ctaggttatc atttaaatat gaaaaataaa tggaagtatg agattgaata
       61 aaaagaaaag cagataaaac ggcaatactt tataccgctc ttcacgaatg gcaaagaagc
      121 cacaaataac gatatatacg tcaatgataa aacgcaaaat ccaagctgct gtcataaaac
      181 tgttcaaatg cccatggagg ttaaaataac catacatacg aatataaaaa tagctgaata
      241 ttaaatgtgc taatgaaaga aaagcaatac taagtgtaaa aaaaagatgt ttcttctgca
      301 tgattgttcc agtcccttac taagatagat agtctttatt ataccacgtg tgaggggaga
      361 aataaaaagc cgttttttag tagatatgaa acaaaacaaa aacccgcatt tattcaacct
      421 attcatcctt ttaaaggata gattgaacgt agatagaagt cgtttatcca gtctttttca
      481 ttttgaaaaa aagctaacag catgcttttt ttatggtata ctagcaagga aacgaattcg
      541 catcatgcga taaaggagga agagccccgt ggcagaaaag gaaacttttt atatcactac
      601 gccaatctat tatccaagcg gcaaacttca tatcggcaac tcgtatacta cgattgcatg
      661 tgacgcaatt gctcgttata aacggttgat gggctttgat gtgttttatc tcactggagt
      721 ggatgaacat ggtcaaaaaa tcgaaacaaa agcagaagaa ctaggtgtaa aaccgcaaga
      781 atatgtagat aaaatggcgg ctgatgtcaa gaaattatgg aaaacgcttg atatcagtta
      841 tgataaattc attcgcacga cagatgatta ccataaagca gctgttcaaa aaatatttga
      901 tcgtttatta gaacagggag atatttactt aggtgaatat gaaggctggt actctgtttc
      961 tgacgaagaa ttctttacag aaacacaact tgcagaagtg tacaaagatg acgaaggtaa
     1021 cgtgatcggt ggtaaagcgc caagtggaca cgaagtagaa ttggtaaaag aagaatcata
     1081 cttcttccgc atgagcaaat acgcagaccg tctattggaa tactatgaag aacatcccga
     1141 attcatccag ccagaatctc gtaaaaacga aatgatcaac aactttatca aaccaggatt
     1201 agaggatctt gcagtttcac gaacaacttt tacttgggga gtaccagtca agagtgatcc
     1261 gaaacatgtg gtgtatgtat ggatcgatgc attatccaat tatattactg cactaggata
     1321 tggctccgaa gatgaaagtc tattcgaaaa atactggcct gcagatgttc agatggtcgg
     1381 taaagaaatc gttcgctttc atacgatcta ttggccaatc atgctgatgg cccttgattt
     1441 gcctctacct aaaaaagtat ttgggcatgg ctggctattg atgaaagacg gaaaaatgtc
     1501 taaatcaaaa ggaaatgtcg tttatcctga aatgcttgtg gaacgttatg gtttagatgc
     1561 actccgttat tatttgcttc gtgctgttcc gtttgggtca gatggtgtct ttacgccaga
     1621 agattttgtt tcccgtgtca attatgatct ggcaaatgat ctagggaact tattgaatcg
     1681 aacgattgct atgatcaaca aatattgtga tggactcgtt cctgactatg cctcacttgt
     1741 cactccattt gatagtgaac tgtctacgac ggctgctaac gttgtcggac gttaccatga
     1801 tgcaatggaa aaaatggaat tcaatacagc attggctgaa atctgggtgc tgatttctcg
     1861 tgcaaacaaa tacattgatg aaacagaacc atgggtatta gcaaaagacg aagaaaagaa
     1921 agctgaattg aatagtgtca tgatccattt agctgaatct ttgcgtatcg cagcgatatt
     1981 acttcagcca atcatgacag aaacgccaga gaagatcttc aatcaactag gattggactc
     2041 tgaaacaatg aatcttgaag gattacactt tggcgaattt ccttcaggaa caaaagtagt
     2101 tgcaaaaggt acaccaatct tcccacgttt agatatggaa gaagaagttg cttttattca
     2161 agaaaaaatg tctgaaggta ctcaaacaaa cgaagataca gtcaaatggg atccagaaga
     2221 aacagaactt gtttcaacga aagaaaaaca aatcaaattt gatgtatttg aaaaagtaga
     2281 actaaaagta gcagaagtca tcaactgtca aaaagtagaa ggggcagata agttgctgca
     2341 attccgacta gatgctggag acagccaaga ccgtcaaatc ttgtcaggaa tcgctgaatt
     2401 ttatcctgat ccaagcgaat tgatcgggaa aaaagtcgtc attgtagcaa atctgaaacc
     2461 acgaaagatg cgtggacaaa tcagtcaagg aatgatcctt tctgcagaag caccagatgg
     2521 ctctttacaa gtaatcgaag caccaaaatc catgccaaat ggctcagaaa tcgcatagaa
     2581 attcaaaggg ggtatgacac tagttgtgtc ataccccctt tttccgaaat ttgagaagca
     2641 attttcgtaa attccttctt attacttgaa gccaatcata taatacagaa acagagaact
     2701 atccaaaata aatcctagaa aaagaagtac gagcttcgct gatttttgtt aatttagaat
     2761 aatactaata ggaatcgtaa aaacagaaaa aaagcataat gataaagggt cgggcagatt
     2821 ttaattgatt catctggtac tcttttttat tgtccttaca ttcataaagt ttgaatgcaa
     2881 tgactttatt acatctatat ttatatctaa cagtagcgtt gatgtgttaa ttataagttc
     2941 tatcacttta tgttcaatta gtttgtgctc ttatatgtgt gattgaaaat atagataaaa
     3001 aaacaatcat cggctttgac ttcgatgatt gttttcttta ttttgtaaaa atcctatcac
     3061 ttgattttat ggtaaccttt tatttttgtc ttcaaacggc ttggtaagag agcagtaagc
     3121 agtgcaaatg ggatcgaaaa gacataccag aaaatcgtac gtccgatacc ataatgttga
     3181 tagagctttg tcaacatcac gtgtaacaag atcaacccta aaagcaagga gccagctata
     3241 aaccaatgat tatcccaatg catgacttca ctaagtgcca tcactccaaa aggatagaaa
     3301 taaaaataga tcccgactaa ggtcatccgc cagttctttg taaatgttcc gatattcaat
     3361 aaaaagaaaa tcgctgaaag caaaataccg ataggcggaa aaagcccaac caaaaaggaa
     3421 tacaacatcc cccacaagat catcacagga cctttgatca atgcaacgat tgcgatgaac
     3481 aaagtcagca atattagttt ttgtgataac gttgcaacag tcattaatcc aaataaaatt
     3541 aatgtgaacc aatgccaatg ggactgctgt tgttctttac gggaaaaatc gacccggttg
     3601 gataaataat ctgctaattt cggataagaa cgcttaaaat ccataaactg ttcctccttc
     3661 gtggaaaagg taggtttagt atacgaaaaa taaaaaagaa atacatccgt cttaagaggg
     3721 agtttttcac tgaaagtgga gaaaaaagaa tggtaaatta aaatattctc agttatactg
     3781 aaaattatac caaatttcaa agaagaaaga ggatgggaat gaaaggcaaa ctaaaacgag
     3841 aaatcaatct gtttggcgca ttagctaccg ttatgggaac cgtgatcggt gcaggcgtct
     3901 tcttcaaaac tgctgctgtt actgctagta ctcagtctac aagtttgaca ttgctagctt
     3961 ggcttttggg tggtttctta acgatctgtg ctggattgac agtggctgaa ttagctacag
     4021 caatcccgga aactggtgga gctgtaaaat atattgaagc tgcgtatgga aaattgccca
     4081 gctttttatt aggatgggcg caaagcttga tttactttcc tgctaatatt gctgcattat
     4141 cgattatctt tgcgacacaa ttgacgaact tattacaatt atctaccgat tatcttttgt
     4201 tgattgcagt gatcaccgct gtttctgtga cagggttgaa tttgttaggg acaaaagtcg
     4261 gtgcttctgt acaatcagtg acgttgattg ttaaattaat accaattgct gtgattgtca
     4321 tatggggatt attgacccca ggacaaggaa ctgtccagtt atttccaatc gaagctggaa
     4381 aagatgttac ttttgtcgaa ggattgagca gtgcgttgtt agcaacgttg tttgcttatg
     4441 acggctggtt aggtgtcggt gcaatggcag gggaaatgaa acggccggaa aaagatttgc
     4501 ctaaagcaat tattcttggt ttgagttttg taacagtcgt ttatctattg atcaattttg
     4561 tttttttgaa aactttgccg attgatcatc ttgccggtaa tttgaatgca gcttctgaag
     4621 catcagacgt catttttgga ggaatcggtg gaaagctggt cactattggg atcttgatct
     4681 cggtatacgg tgcattgaac ggttatacac taactgggat ccgcgtgcct tatgcgatgg
     4741 ctttagaaga cgagctacct ttcagtaaac agctgacaaa gctttcaaag aagtttacgg
     4801 ttccttatgt tcctgcggtt ttccagttag cagtagcttg tattatgatg agccttggtt
     4861 ctttcgactt tttaacagac atgttgattt ttgtgatgtg gctattcagt ttactgattt
     4921 ttatcggtgt ctttatttta agaaaaaaag cgccggaatt gccacgtcca tataaagtac
     4981 cactttatcc aatcgttccg attattgcga ttctcggtgc tattttcatt ttaggtatga
     5041 cgatgatgac gcaaacgaaa ttagcattga tcggtatagg tgttaccttg ataggtatcc
     5101 cagtctatta tcaaaaaaag aaaaaacgct cagaagagta aaatatttca acaacagaca
     5161 gaaaaagtac gaactaggtc ctctagatct tcgtgctttt tctatttcaa taaaacaagt
     5221 tcttctattt tgatttttgc tacttctgca agtagtgctt atgtgctaaa gtaagaatga
     5281 aaaggagaga taaacatgat atttgattct catacccatt taaatgctga acagttcaat
     5341 gaagatattc cagaaacgat tcagcgagca caagaaatgg gggtaacgaa aatggcggtc
     5401 gtgggcttcg atacgccaac gattgaaaag tctttgcaat taagccatga ctatccaaat
     5461 atatacagta ttatcggctg gcatccgaca gaggctggca gttatacgaa agaaatcgaa
     5521 aataaattac aagaacaatt gactttacca aaggttgtag cattagggga aattggcttg
     5581 gattatcatt ggatggaaga cccgaaagaa gtccaagata aagtatttcg cagacagatt
     5641 gctatcgcaa aagaaatgaa cctaccaatc agtattcata ctagagaagc acttgaagac
     5701 acgtatcaaa tcctaaaaga agaagatgtt cgagatatcg gtggaatcat gcatagtttc
     5761 agtggtgatt atgaatgggc aaaacgattt cttgacttag ggatgcatat ttcatttagc
     5821 ggtgtcgtca ctttcaaaaa agcattagac gtccaagagg ctgcagcaaa tgtaccaatc
     5881 gaacgattac ttgtggaaac agatgcccct tatctggcac cagttccttt tagaggaaag
     5941 cgaaatgaac caggctatac tcgttataca gtagaaaaga ttgctgaact tcggaatctt
     6001 ccgacagaag aagtggctgc ccaaacatgg acaaatgcgc accgattatt tggtctaagc
     6061 gaacatggat aagatgaaga tccaagagat tatagtcgtt gaaggaaaag acgatacaag
     6121 aaggctgcgg gaagtagttg atgcagatac gatagaaaca atagggtctg caatcaatga
     6181 agagatcctg atgcaaattg aacatgccca agagactcgt ggagtgatcg tttttaccga
     6241 tcctgatttt tcaggagaaa aaataagaaa aatcattatg gaagctgttc ctcaggcaaa
     6301 acatgctttt ttgccgcgca cacaagctaa accaaagaaa aagggaagcc ttggtgtcga
     6361 gcatgcaagt gatgctgcta ttttagaagc attgaaaaag gtcgtgacgc cagacatttc
     6421 ccaaacggct gtttctgaaa tcactagaca agacctttta gactatggtt taatagctgg
     6481 ggcacaagca aaagaaaaac gagaaaaact aggtgacgag ttgcgcatcg gttacacgaa
     6541 cggcaaacaa ttggaaaaac gtttgaagat gtttcgtatt acgccgaaag aactcgccca
     6601 agcaatgaaa atagtggagg aacaacatga gtgaatatcg tgaaattgcg acaccctcta
     6661 gaacaaaaga gattttattg aaacatggtt tttcatttaa aaaaagttta ggacaaaatt
     6721 ttctgactga accaaatatt ttaaggaaaa tcgtagaaac tgcgcaaata gatgaacata
     6781 cgaacgtgat cgaagttgga ccaggaatcg gtgcattgac ggaacaatta gccaagcatg
     6841 caaaacaagt cgtagcattt gagatcgatg atcgtttgat ccctgtattg gctgatacga
     6901 tgcaacccta tgacaatgtg actattgtac atcaagatat tttaaagacc gatttgtcaa
     6961 cagctgttag agaaacattt catgaggagc ttccgttaaa agtcgtagcg aatttacctt
     7021 actatattac gacaccgatc atgatgcatt ttcttgaatc agatttggtt gtggatgaac
     7081 tagtcgtcat gatgcaaaaa gaagtagctg atcgtatctc tgctgaacca ggaacgaaag
     7141 cttatggttc attatcgatt gctgttcaat attatatgga agccagttta gcattcatcg
     7201 taccaaaaac agtatttgtc cctcagccaa atgtagattc agcgattttg aaactgacaa
     7261 gaagagatat accggcagtc gaagtgacag acgaaaaagc atttttccga ttgacaaaag
     7321 cggcatttca gcaaagaaga aaaacgcttt ggaataactt gcagcattcg tatggcaaag
     7381 atgaccagac aaaagcatgg ttagcaaaaa gcttggaaac agctggaatc gatccaaaac
     7441 gtcgtgggga gactttgtca ttacaagaat tcgccgcatt aagcaatgcg atgtcagaaa
     7501 atatgcagta aaaaaagagg ttgtgacaga agttgtgaaa aagctgtcct gcatcaagta
     7561 ataagaacag ccaaacaaaa atagttcctc atgtttttcg tttgtttggg cttattaccg
     7621 cagatgcaag cttttgaaca ccgtttaaga aagaggttgt gggcctgccg tttaactcta
     7681 agtcacaagg aacaatttca aaaaacagct tctcatgttt ttgagttagt tggtcttatt
     7741 acttgatgca agcttttgac cactattatg ccgaacaagg agatgtgaca caaattttgt
     7801 cacatctcct atttttgttt cttgatcaaa aaattcttat acttgatact cacactcatt
     7861 ttgtatggat ttagaaaaga atatgtgtct tgacagttat tagtaagtga gttatactaa
     7921 gtcgacattt atgtgtcaag acatattagg agagagaaaa aatgagaaaa aattcagttc
     7981 gaaaactaac gatttctgct ttattgattg ggatgggggt cattatccca atggtcatgc
     8041 ctaaaatcat gattccacca gcttccttta ctttggcgtc tcatgtgcca ttatttattg
     8101 cgatgttttt tacaccagga gttgcagtgg ctgttgcgct aggtacgaca tttggtttct
     8161 ttttaacaac gccggttatc atcgcattgc gagcattgtc acatcttgtg tttgcgttga
     8221 ttggggcttg gtacttgcaa aagcatccga gtatcgtttt aaaaaatgga cagtttactt
     8281 ttgctaattg gcgtttccaa gggttcaatt ttgtgatcgg tgtgattcat tctttagcag
     8341 aaatgctagt agtcagcatc ttttacttta ttggtaatat gcctgaaaca tattattctc
     8401 aaggatattt ttatacagta ttcatcttga tggggattgg tgggcttatc cacagtttag
     8461 ttgattataa tattgcttat tttatagcag gaacactcag caaatatttt gatatccccg
     8521 tatttacggc agcaaaaaaa gaaaatcgga aagagtcgat tcaagggtac gcgaagaaaa
     8581 cgacatatta gacatgatct ctcaaaaaaa aaactctcat ctagtaatgg ataataaatc
     8641 ctctaagatg agagtttttt gtcctaaaaa gagctgcacc aacatcttct ggcacagctc
     8701 taacaggggg caatttatgg aagtttgatg acttgttctg tttctttatc aaagaagtgg
     8761 cctttgctga tgttgaaggc aaggtcgact atttcgcctg gtttatggaa atctcttgca
     8821 tctacttttg agatgaactc agtagaacca gtttttgtgt aaagcatcgt ttcagctcca
     8881 agtaattcgg aaacgacaac ttctgatttc actacggctt caggtgaagc ttcaagggct
     8941 acttgttcac tgtggatgtc ttctggacga ataccgaaga taacttcttt tccttcataa
     9001 cctttttcaa ctaatacttt gttttttcct tcaggaatac gaagcttaag cccatgtcca
     9061 tctgtgataa caccattttt caatgtcaca ttaaagaagt tcatggcagg agatccaata
     9121 aagccggcaa cgaacatatt gacgggtgta tcataaactt ctttcggtgt accgatttgt
     9181 tggataaatc cgtctttcat gataacgatt cggtcagcca tagtcattgc ttctgtctga
     9241 tcgtgagtaa catagatggt cgttgtttca agacgctggt gaagtttggc aatctcagca
     9301 cgcatggcta cacgtaattt agcatctaaa tttgataatg gctcatccat taagaagact
     9361 tttgcatccc gaacgattgc ccgtcccaat gccacacgtt ggcgttgtcc accagataaa
     9421 gcagctggtt ttcgtttcaa gtattctgtc agacctaaga tctctgctgc attgtccaca
     9481 cgttttttga tgtctgcttt atcatattta cgtaatttca agccaaaagc catattatca
     9541 aaaacagtca tatgaggata caatgcgtag ttttggaaaa ccatggcaat atcacggtct
     9601 ttaggtgcca cgtcattcat gacttttccg ccgatattta gttctccttc agagatatct
     9661 tccagtcccg cgatcattcg caaagtagtt gactttccac aaccggaagg accaacaaaa
     9721 acgataaatt cacggtcttt gattgataga ttaaaatcag ttacagaata attttctgca
     9781 ttgtcatatt ttttatatac gtttttcaga gccatttcta ccataatacg ttccacactt
     9841 tcttttttag ttttattaac tatttaaagt ataaatgaaa gcgttaacta acttcaatgt
     9901 catgttgaac aagaattttt tctttttttt gtcaatgtgc tagttttaag ctttaataaa
     9961 tatccataag aaataaaaac tgatacactg gagtaaataa gtatgttaac aatatcaaaa
    10021 ggagtgtctc ttatgaaaat cgctttgatc gctcatgacc gaaaaaaacc tatgatggtg
    10081 aaattagcaa cagcgtataa aacaatattg gaaaaacatg aactttttgc taccgggacg
    10141 actgggcaga ggatcacgga agcaaccggc ttgccgatcc atcggtttaa atccggaccg
    10201 ttaggcgggg atcagcaaat tggagccatg atttcggagg atcagttaga tttggtgatt
    10261 tttcttcgag acccgctggc tgcacagccc catgaaccag atgtaacagc acttatccgt
    10321 ttatctgatg tctatgagat tccgttagct acgaatatcg gaacagctga ggtgctacta
    10381 agaggactgg aagccggctt cctagatttt cgaagaatcg tccatgaaat agacaagcat
    10441 ccactttcat tttgatggat aagcgtatgg aaaaataacg tttaatgttc gcctatatgt
    10501 agctataaaa gctaaacaaa ctgataatat gcgatataat acgaacgtat tggataatat
    10561 aaacaataat attagttttt cgtatttgca gtaaatgtga aaatatgcta tagtttctgt
    10621 gggaaaagat cccaaaaata atacagagta gaaaataaag cgttaagtgc taagaggatg
    10681 ggatgttgcc tcttggacga aggacgtaat gttcgcggtt ttattttcgc attcgctgca
    10741 tgatacgatg tagcagctct taaaggagat gctaaaaatg aagaaacacc ggttagacgc
    10801 acgtatgatt gctattatgg gcttattgat tgcattgatg gtcactttgt cccgactggt
    10861 agcaatcgag accccattta ttaaaatcag tgtcacattt attccacaag tgatcatggg
    10921 cattcttttt ggtccttttt ggtcagggat aggcgcagtt ttagctgatt tagtaggaat
    10981 ggccctcttc tcgaaatctg cattctttat tggatttaca ttgaatgcat ttattgaagg
    11041 agcaatctac ggattctttt tctatcgaaa agagatcact tggaaaaatg ccattctggc
    11101 tacgcttagt gtcacgctga tcatcaactt atttttgaca ccattatggc ttgcgttgat
    11161 gtaccatgtg ccattgttca gctgggtcgt ttgggcgccc cgcctgttaa aaacagtaat
    11221 atggttaccg attcaatcaa ttgccattta ttacgtagga cgtagtattc cttacaaaaa
    11281 aattttaaga agtttagcga tccacgctaa ataaaaaaca agcagtcttc ccatcccaga
    11341 agactgcttg tttttattgt gctgtgaatt gtgcttgata gagccgtcgg taataaccat
    11401 ctttttgaag cagctcatca tgggtgccca tttcgacaat agctccctga tccatcacta
    11461 atatctggtc cgcatttttg attgtggaca ggcgatgggc aatcacaaaa ctggtttttc
    11521 cttccatcat tgctaagaag gcttgttgga tttttttctc tgtcaatgta tcgactgagc
    11581 tggtagcttc atctaagatc aacatagggg gattactgat catcgttcga gcaatcgtca
    11641 acagttggcg ttgtccatct gatatcttta acccttgacc gccaatctta gtatctaatt
    11701 tttccggcaa acgttctaca aattcataca tataagaggc ttttagggca tcattgattt
    11761 ctgcgtcact tgcttccgga ttcccaaaac gaagattttc tcgcaatgta ctgtcaaata
    11821 gccaagtatc ttgtaacacc attccaaaac ttttacgtaa tgtatcacgt tggatagagc
    11881 ggatgtcatg gtcatcgact gtaattgttc cttttgttgt gtcataaaaa cgcatcagca
    11941 aattaacaag tgtggatttt ccggcccctg tttttcctac tatagcaatg gtttgacctg
    12001 gataagcagt aaaattgaaa tcttcgatca aacgctgctt ggggtcataa gaaaaagaaa
    12061 catgatcaaa agagactttt cctttgatat ttttgggttc caaggcatag gcatctggca
    12121 aatcaggctt ttcttctggc tgatcgatca aatcaaatgc tcgttctaga ccagaaaaag
    12181 cggtttgcag ttgtgtcgtg attccagaaa gttcgataaa tggtttagaa aactgacttg
    12241 agtagatcgt aaagctggaa atcacaccaa cagtgattga cgaaccactc ttcaatgcca
    12301 ataggccgcc aactaaacca atcgataaat aagcaaggtg atctacaaaa cgagaagacg
    12361 ggtttgtcaa ggaagaagaa aattgtgccc gttgtccttt ttcatataga tcagcattta
    12421 gcagttcaaa ttgctgttga ctagcttctt cttgctgaaa agctttcacg attttctggt
    12481 taccgattcg ttcggtcaca aagcccgaga tatccccaat aatcctttgc tgtctatcga
    12541 aatttgtttg tgaagcacga gcaacgatcc aactcacaag gaaaataatt ggtgtagaac
    12601 aaagaacgac caatgtcaga agtggactta gacgcatcat aaaaataaac gaaagaatga
    12661 tgattgccac tccagaaaat aattgattga aggccgcaga aattgcaatg gaaatattgt
    12721 ccatatcatt tgtaaatcga ctgacgatat ttccgtggga gttttgatca taataacgaa
    12781 gtggcaatcg attcagatga gcaaatgcat cttttcttaa ttttgcaacg gataagtaag
    12841 ctaaccgatt gcttaacact tggatgagcc attgactgac tacgttgatt aaaacgatac
    12901 caccgaatag aataagtaaa tggaacagtc gattgaagcg gacctgtcct tctccaatca
    12961 tttgatcgat actttgacca atttgtaacg tcatcacaac tgttgcaatc ccattgatga
    13021 ttcctagtat cacagctagc caaatgtctt ttgcataggc agtaaggtaa ggaatgaatc
    13081 ggcggagcgt ctgcaaggat aattttttta ctttcataac tattcttcct cctgtgatgc
    13141 gacgatttcc tgatattctg ctgattgagc aagtaattct tcatgggtac cctgagcagc
    13201 aatcttacct tgatccatca ctaagatatg atgtgcttct tgaatagagc ggacacgttg
    13261 ggagataata atcactgtac tgccaagata agtctgtagc gccttacgca aatccaagtc
    13321 tgtttgataa tccaacgcac ttaaagagtc atctaaaatc agtaatgctg gcttggcaat
    13381 caaagctctt gcaatcgtca gtcgctgttt ttgtccgcca gagaaatttt tcccattttc
    13441 catgactttt gtatctaaac catttggaag ctgacggaca aattcttctg cctgagcgat
    13501 tttcagagct tcccaacatt ctttttctgt ggcgtctttc ttaccccatt gaaggttttc
    13561 acgaatagtc ccggtaaata gtacagaagt ttggggaaca agagcaattt gctgacggag
    13621 atgatccagc tgccaagaac gaacatccat tccgtccatt ataactgtac cggaactgac
    13681 atcatagaaa cgcggaatca attgtgtcaa agtcgttttc ccgcttccgg ttggaccaat
    13741 gattccaagg actgtatttc gcttcagctg aaatgtaata ttttggagag aaagaccaga
    13801 atcaggagta taacggaagt caacatggtc aaaagcgata attgcttcag aagaaaaatc
    13861 gggagtagta ctgacctcag cttcaagaat cgaattttct gtgtctagca cctctttcac
    13921 ccgatttcct gaagcttcgg cacgtgtgaa gatcacgacg aggtttgaaa caacgatcag
    13981 tgccaaaagc atttgattca tataattgat caaagcaaga acttgtcctt gttgcaagct
    14041 accaatattt actttgatcc cgccaactgt cagcaaacag atgatcccga cattcatgat
    14101 caaggttgta gcaggtgata atagggcaga gatattggaa acacgtaaat aattgttcgc
    14161 tagatcattt gtgcttttat cgaacttctc tgtttctttt tttgtctgcg caaatgcgcg
    14221 gatgacacga acaccgctaa gattctgact aacgtgttca ttgagctgat ctagtttttc
    14281 ttgcactttt tggtagagag gaacggtttt tttgatgata aaaaataaaa tgattgagaa
    14341 aataggcaat aatgctaaga aaaacagacc tacttcccag tcgatcacaa aagccatgac
    14401 cactgacccg atacttaaaa acggtgctct gatcaataaa cgaatgacca ttgctaaagc
    14461 tagctgaagc tgattgatgt cgtttgtcat tcgggtgatc aaggtatctg ttccaaaatg
    14521 attcaactcg ttgtgagaga aagtattgat ttttttgatt agctgattac gtaactctgt
    14581 accaaatcct tgtgaagcta ctgaagcata gtattggcaa atgatagcac aaatcagacc
    14641 aatcacagac atagcgacca tccataagcc catttcaatg atttttcctc ggtcattcat
    14701 ttttagtcca ttatcgatca aggaagccat cattaaaggc aggacaagtt cgaagacagc
    14761 ttcaagaaat ttgaagaacg ggcccaaaat gatttgctta cgatagtctt ttgcataacg
    14821 taataactga atcatatgat tcctcctatt atttatttgt ccacaattga aaaaacaagt
    14881 actaccacat tgtagcttag gaaatttaga atgaaaagag aacaagcctt ttcataaatg
    14941 aaagttttat tatgaagaag ctttagaaat tgaatagact atttttttta aaaaaaagaa
    15001 aatgagaaaa catgccagtc tcttcattca taggaacgtc tattttactt
//
LOCUS       DAZKKW010000064        14821 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.065, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000064 DAZKKW010000000
VERSION     DAZKKW010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14821
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.065"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(51..931)
                     /locus_tag="ACG8PO_001946"
                     /pseudo
     CDS             complement(51..931)
                     /locus_tag="ACG8PO_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
     gene            complement(1177..3174)
                     /gene="tkt"
                     /locus_tag="ACG8PO_001947"
     CDS             complement(1177..3174)
                     /gene="tkt"
                     /locus_tag="ACG8PO_001947"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFQ0207253.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPIGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(3276..3527)
                     /locus_tag="ACG8PO_001948"
     CDS             complement(3276..3527)
                     /locus_tag="ACG8PO_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HFQ0207254.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            3674..4297
                     /gene="lexA"
                     /locus_tag="ACG8PO_001949"
     CDS             3674..4297
                     /gene="lexA"
                     /locus_tag="ACG8PO_001949"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HFQ0207255.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            4476..4694
                     /locus_tag="ACG8PO_001950"
     CDS             4476..4694
                     /locus_tag="ACG8PO_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0207256.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
     gene            complement(4982..5494)
                     /locus_tag="ACG8PO_001951"
     CDS             complement(4982..5494)
                     /locus_tag="ACG8PO_001951"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFQ0207257.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(5508..7805)
                     /gene="recJ"
                     /locus_tag="ACG8PO_001952"
     CDS             complement(5508..7805)
                     /gene="recJ"
                     /locus_tag="ACG8PO_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFQ0207258.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSN
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     IMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(7947..8390)
                     /locus_tag="ACG8PO_001953"
     CDS             complement(7947..8390)
                     /locus_tag="ACG8PO_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA
                     domain-containing protein"
                     /protein_id="HFQ0207259.1"
                     /translation="MKELKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPL
                     ILVIIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQRE
                     FRNERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(8391..9179)
                     /locus_tag="ACG8PO_001954"
     CDS             complement(8391..9179)
                     /locus_tag="ACG8PO_001954"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HFQ0207260.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(9176..10117)
                     /gene="rnz"
                     /locus_tag="ACG8PO_001955"
     CDS             complement(9176..10117)
                     /gene="rnz"
                     /locus_tag="ACG8PO_001955"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HFQ0207261.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(10294..10881)
                     /locus_tag="ACG8PO_001956"
     CDS             complement(10294..10881)
                     /locus_tag="ACG8PO_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HFQ0207262.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(11102..12415)
                     /gene="obgE"
                     /locus_tag="ACG8PO_001957"
     CDS             complement(11102..12415)
                     /gene="obgE"
                     /locus_tag="ACG8PO_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HFQ0207263.1"
                     /translation="MSMFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGG
                     DVILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAET
                     GALLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVL
                     ADVGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLE
                     RPQIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNAT
                     ADLLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTN
                     FDHDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            12703..13713
                     /gene="gap"
                     /locus_tag="ACG8PO_001958"
     CDS             12703..13713
                     /gene="gap"
                     /locus_tag="ACG8PO_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFQ0207264.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(13798..14733)
                     /locus_tag="ACG8PO_001959"
     CDS             complement(13798..14733)
                     /locus_tag="ACG8PO_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFQ0207265.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
BASE COUNT         4107 a         3307 c         2616 g         4791 t
ORIGIN      
        1 aaagacaaag atataccata tatggtagtt tctttgcctt tttatctaaa tcaaacgttt
       61 agttcttgac caactaataa agaataactg gacatagaca tgccattttt cgccattaga
      121 tcatcaacag acatgccaaa tttttgtgca atatcccata gcgtatcacc tgacacgact
      181 gtatatgtct gaccattatt tgtatttaca tctgttgttg aagatgatga cgttgaagtc
      241 gtcgtagtag tcgttgttgt tgtacttgtg gtagccgaac ctgttgatgg cgtatcaaaa
      301 cgggttaaat tataagtatt aatcaatgag ttcaatttgg ccccataatt tggatcggta
      361 gcataacgac ctgtcaagta cgctgttgca tcataaaaac ttgttgtatt gcttttccac
      421 acgccagcat aatgataatc gcccgtggaa aaactggttg aacgaagaac attcgcatgg
      481 tcttggaagg attcccaata agaagaatat tttcggaatg gttcattcat cgttacccat
      541 tgtccgttac atattctttg gttgccatat aaacgacctg tccgttatag cttcctttca
      601 caccgaatag attataattt ggcgcactag ctaatccgga agtgccatat gcactttcca
      661 acaacgcttg agcgatcatg actgaagcat acaagtcatt acttgcagca acttctgacg
      721 cagaccagcc gatttgatcg ataaacgctt gaggatcagc aactgtttga gatccttgtt
      781 catctgcatg aacatgttgc ggtaccgctg taaccatcat cggcccaact aaaagactcg
      841 ttccaattaa aggagcaatc gcttttttat tgatcaccat ttttctttta ggtctatggt
      901 gtcgggcact gcgggtcttc agctcttgca ttcaaattcc tcctcaaaat aaaaaaactt
      961 aataaaaagt tctcttctta aaacaaactt aattaattaa attatacttt taattctcaa
     1021 gaaacacaaa ctaatccgaa gaattctttg ttttgtcatt taaatgtaac atagccgaaa
     1081 acgaaaagta atattgattt attcttctaa cacataaaga acagctgtct ccaacttgca
     1141 gacagctgtt cttatcagtc aaacaattgc ttggatctat gatagttgat tgtacgtatt
     1201 cacgacattt tctactgtaa atccaaattc ttctaaaatt ttagtgccag gggcagatgc
     1261 accgaaatga tcgattgtaa tcgttgcgcc ctctgttcct acgtagcgtt cccaaccaaa
     1321 tgatgcagca gcttcgatag ccacacgttt cttcactgat ttaggaagta cactttcttt
     1381 atattcagaa gattgttttt caaagaggtc aaagcttggc atagaaacaa cagaaacatc
     1441 tttgcctttt tcagccaatt ctttttgagc tttcacagcc aaatcaactt cagaacctgt
     1501 tgcaatcaaa ataccttctg gtgtttctcc ttgtgatgga gaaagaacat acgcgccttt
     1561 tttcaccatt tcatctgcta cttcttttgt agaaggcaat actggaaggt tttgacgact
     1621 caatacaagg atagtaggcg catctgttgt ctccatcgct actttccaag cagcacgtgt
     1681 ttcatttccg tctgccggac gaatcacttg aacaccaggc atgcagcgaa cagatgctag
     1741 ttgttcaatc ggttcgtgcg ttggtccgtc ttctccgaca gcaaccgagt catgtgttaa
     1801 gacgtaaaca acaggagtat gttggattgc tgctaaacga actgctggtc ttagataatc
     1861 gacaaagaca aagaatgttc caccgtagat acgagttccg ccatgaagtt ggataccatt
     1921 catagccgaa gccattgcaa attcgcggac accgaaccag atgttgcgtc cttcataatg
     1981 ttcaggagtg aaatctttat ctgcagccac cattgtatta ttggaacctg acaagtccgc
     2041 tgaaccaccc cagaagcttg gtatagcttt cgataattct tggatgactt ctttacttga
     2101 aacacggctt gcttgactgc ttccaacttc ataagttggc aattctgcat cccagttttc
     2161 tggtagtttg ccatcaaaag catcttcaaa ttgttgtgct aattcaggat aagctttttt
     2221 ataattggca aacatttcat tccatgcatc ttcagctttt tgtccttctt ctatcatcgt
     2281 ttgatggaag cgggcagcta cttcttctgg aacagtgaaa tctggatact cccaaccata
     2341 aacttcttta gccatcttga tgccatcttc accaatagga gcaccatgaa cagcagatgt
     2401 tccttcttta ggagcaccat agccgatgac tgtttttact tcgatcaatg ttggtttgtc
     2461 tgtttcagct tttgcttctt caatcgcttt agagattgct tctaggtcat ttccgtcttt
     2521 cactaagata tgttgccagc catatgcttc atagcgagca ccaacgtttt ctgtgaaagc
     2581 ttttgaagtt ggaccgtcta gtgaaatatc gtttgaatcg tataaaacga tcaatttccc
     2641 tagtttcata tgacctgcca tcgaacttgc ttcttgagat accccttcca tcaagtctcc
     2701 gtcaccgcat attgcatacg tatagtgatc catcacatta aagttttctt tgttatacgt
     2761 tgctgcaagg tgcgcttctg ccattgccat accgacagcc attgcaatcc cttgtcctaa
     2821 tggccctgta gtggcttcta caccatctgt gtgatgtact tcaggatgtc ctggtgtttt
     2881 agaatcccat tgacggaatt gtttcaaatc atcgattgtc acttgatagc cagctaaatg
     2941 aagcaagcta tacaacattg ctgacccatg tcctgccgaa agaacgaaac ggtcgcggtc
     3001 agcccagttt tttgatgtcg ttggatttac tttcaggtgt tttgtccata acgcatatgc
     3061 cataggtgct gcgcccatcg gcaatccagg atgtcctgag tttgcttttt ggactgcttc
     3121 aatacttaat gtacgaattg tgttaacgcc aagttggtca attttgtcaa acaaaataac
     3181 ccctcctatt aatttaggtt ttttttacct ttatttctta cactttcatt ctataagatg
     3241 tcacacgaaa aagcaatctt tttcagtatt ttttactaaa agttgtcatt tcggttgtgt
     3301 aagccttttt tcttttggat ttcttttaat ttttccgggg tcacgtcatt gccctcagga
     3361 tcgacgactt tcattccttc gatatgatga cgcataccgg aacgaaaagc ttccaaatac
     3421 tctttgcgta agttgatttg ttcttgttct tcggcttttg agagaccatt ttccttcttt
     3481 ttacgtgcaa gttcattgat cctctctatt ttttcaggtg acagcatgct cagccctcct
     3541 tttactctcc ttcatattat ataaaaactg aaaaaaaaac aactagataa actaaataaa
     3601 atacgaacat tcgtttgatt tagcgctttt tatatggtac tatttattta tcaagtgaaa
     3661 gaaggtgtac cttgtggccc gacaaactga atcaagacaa atcgaagtct tacgatatat
     3721 ccatgaacaa gtaacccaaa aaggataccc gccgactgtc cgtgaaatcg gggaagctgt
     3781 tcagctttct tctacttcta ctgttcacgg tcatcttgca cggcttgaaa aaaaagggtt
     3841 tatccaaaga gatccgacga aacctcgtgc aatcgagttg acaaaacaag gtttagaaaa
     3901 gattgggatt cgtccaacaa ctattccagt acttggcgtc gtaacagctg gagaacccat
     3961 tttagcagta gaggatgctt cagacttctt tccaatccct cctaatcttt cttccgaaga
     4021 aggcagtctc tttatgctga caattcgtgg agatagtatg atcaatgctg ggatcttgga
     4081 tggtgacaac gtgatcgttc ggaagcaaga aactgctcaa aatggcgata ttgtcattgc
     4141 aatgactgcc gaggacgaag cgacttgcaa acgattctat aaagaaaaaa actttttccg
     4201 tctgcagcct gaaaacgact tgctcgaacc aatcatctta gatcaagtat ctatattagg
     4261 acgagtagta ggactttatc gtagtcatat ctattaattg ctactaataa aagaaaactt
     4321 tttaaaaaga gtttgagacg atggtctcaa actctttttt cgcccgggtt cttcttcttt
     4381 caaattttta tcacttccct ctttacaaac cgaacaaata ttcgtatact aaatatacga
     4441 acaattgttc gcataaatta acgaggtgag aattcatgaa atcaatgaga cgtcttggct
     4501 tagtattaac tgttttgttt ttaggtatta ttattggtaa tttcggcata cgtccagctt
     4561 tacttgtgtt tgttccgtta tttactttgt ggttcatgtt gtgggatgaa aagaagtacc
     4621 gtcaatctga gcggcgcaga attcatgaag atcatttcta tcatgagccc tactacagat
     4681 ccaggcaatc ataataagga ttgactgcta attattcaaa agacaagaag ttaaactttt
     4741 acgggggtgt gacaaaaatc ggtcacatcc ttttttcgaa taaacggtgt tctaaagcca
     4801 gtttttcggt attaagtcaa attgcggaaa aagtgatttt ttaaacttct actggtcttt
     4861 gcttgtttcc atttataaaa aaatagcgac cctcaaaagt cagatagaga atctgacttt
     4921 tgagggtcgc tttaaaatcg cttctttaag aataacatca agaatagctt cttttttctg
     4981 cttaatattc cattaatgta atgatgtcat atcctttgat tttgtcgcgt ccatggagat
     5041 ccatcaattc gatcaaaaat gcgcagccta cgactacacc gcctaattgt tcgatcagtt
     5101 cgatcgtcgc tttgattgtt cctccagtag ctagcaaatc gtcacaaatc agcacacgtt
     5161 gacctggttt aatggcatcc ttatgcaatg tcaatgtatc tgttccatat tctaatccat
     5221 aagtcacttc gatggtttct cgaggtaact tccctttttt gcggactggg gcaaaaccga
     5281 ctcctaactc ataagcaaca ggacatccta cgatgaatcc acgggcttct ggcccaacta
     5341 ccatatcaat tcgtttttct tttgcataat ttacgatttg cttcgtagct tcccgataag
     5401 cgtcaccatc tgccatcagt ggggaaatat ctcgaaacac aattcctttt gacggatagt
     5461 cgggaatact ggcaatataa tcttttaaat ccactctttg tttcctccta ggttgttaac
     5521 catttcttta tggtaggaat atcgctcaaa agcaaaaatt cctcaacttt gattttattg
     5581 agccgccgct ggtataatct gctctcagtc aaaggatgac tttctgggct agcgactttt
     5641 tgtaaaacac cgttctctat tgtaacaaat ttcaattcaa aaaacacctg tatcataaaa
     5701 attaataatt tttcagggat acgcaaatat tgagctattg cttttatttt atgacgtata
     5761 tcaattcgtt cttgagaatg gatcagttta aacaattttg cgtactgttc tcttgtgcct
     5821 accccattca aataggcctc atcaggcgaa atccccatca gataaacacg attgaatgaa
     5881 taagaccgaa aaacttcttt cagatccgct aaatcatctg gacaatccat aataactaga
     5941 gagttcggtt ttcttttttc aattattgcc tttacagaat ctagtccttc gaaaacaatc
     6001 gcagcttcct ggataatctc aggcacgaat ttcaacgtag aagaatcaaa tgctaaataa
     6061 aggctacctt ccgacgttcg gctctgttca cggaaacgtt ttgcccgcca atcaaatact
     6121 tgcagaccac ccacagaaaa atcagatacc atcagctgcg gtttcttacg cccgttccat
     6181 tcattgatcg aaagctttcc taccacatct acctgattat tcagcagttc ttcttcttga
     6241 gtaccaaaac caaaagcgac cgcatcaagc tgactcgatg cattggaaag gacaaatttc
     6301 agatgctgtt ggtttgctcc gattcttttg atattttctg cttggacttg tcgaaataaa
     6361 aactgaggca aaggattatc cgtaccaaat ggcgccaata gtttcagctc atcaattcgc
     6421 tctactgtta cttcatttgg cagcaatact tcatcaattc tcaactcagg ccctctcatc
     6481 aagtcaattt gatgatcgac tatatattgg ttcatcttct cctgtagtat agtgacattt
     6541 tcacttggca tcgttaaacc gactgctgca tgatgtcctc cgaagtaagt aaataaatct
     6601 cgcatctggt caagcatttc aaaaagattc aaggcttcaa tactacgtcc agatccttta
     6661 gctgatccat cttcttttaa agtaaggaca agcgttggtt tacccgtttc attcattatt
     6721 ttcccagcaa caatgcccaa aacgccttca tgccatcctc ggtttaccaa tagatggata
     6781 tgattctcct tattgaccat cgctaatgct tcttgtgtta tctgctctac gattgctttt
     6841 ctttcttcat tgatctcatt caattttttt gcttgaacgg atgcttcctc ttcatcaaac
     6901 gtcgcaagta acgagacggc aggacgtgga tcgcccatcc gaccgattgc atttagtctg
     6961 ggagcaatcg aaaaaccgat cgttgtttcg tctgcgtccc tcatttttac gccgctttct
     7021 tcaaatagcg cctgaagtcc tatccgttct gaatgatgaa tggcatcgat tcctaatgaa
     7081 acaaggattc gattctcatc agtcaatgaa actaaatcgg cgatcgttcc tatcgctact
     7141 aaatccaaaa attctgcagg cggttcttcc aataatgcag aagctacttt gaatgctact
     7201 ccgactcctg ccaaatctgg aaatggatag tgccccgctg gatgacgagg gtgaacaatc
     7261 gcataagcgt ctggtaaatt ctcaggtagt tcgtgatgat ctgtaatgat cacgtctaca
     7321 ccagcttgct gagcgtatgc taccgcatca tttcctgcta ccccgttatc aacagtgatg
     7381 atcaactgga ttccttcttc gatcttttct tggtataccg attggtttgg tccataccca
     7441 tgctcaaatc gatttggcag gaatacttct acttctgcac caagtaattc cagtgtttct
     7501 ttcattaccg ttgcactggt gatcccatct gcgtcataat caccgtaaac gaggattttt
     7561 tctccactga ttacagcttc ttggattcgg ctgattgctt tatccatatc gtgtatcaga
     7621 tacggatcat gtaattcttg ttcctttgga tgtaaaaatc ggttgatatc cttttcctca
     7681 tggtaacctc tttgccacag taacttaccg acaaatggag ataatttcag ttcacggatc
     7741 atttggagaa aggattcaga aggctctgtc tcgcttaact tccaatcaaa atttgcttgt
     7801 ctcactttct cactcctaac ttagttatta tagcaaagga aatcagcaaa aaaaagcaga
     7861 aagaaagaat aaaaacagat cagatccgct agacagatgt tcagcaggac ctgatctttt
     7921 gtacgaccat ttatgaaata tccctattag tcaaaataat tgaagctttg tttgggcgat
     7981 tgagtttttt gttcttccgg cgctttcaat tgactgattt gtgcggtttt cgcttgtaac
     8041 tccgcctgat aagcagcttc caactccgca cgttcgtttc taaactcgcg ttgctgttct
     8101 tcttttgctt cttcaatttt cttttgaagt tctgtttgat attctgacat ttcctgagtc
     8161 agctgtttga tttgcttttt ttgctgccat agtgttgccg aagcagtcaa aaatacaatc
     8221 agtgcaccaa tcagcgcaga accgatgatc actaagatca atggcgcaga aaaggaagtg
     8281 aagccaaaac ttacaggaac atttttattg ttcaatacag caaaaagaac aataatcaaa
     8341 actaaaacaa atccaagaat cacattgctt tgctttttca attccttcac ctactttttg
     8401 ttaaaaattc cgctggctaa ataatcacca atcgttggaa acagcgtata aaattttgat
     8461 gcaacttcta aaataaacgg ttgattgatt tcccgttttg gatgtctcat attgcggacg
     8521 atcatttttg ccaacttggc aggttctaaa acgatttgtc cgactttttc caaatagctt
     8581 ccgcttggat cagccttatc gaagaaattt gtctcgattg gacctggatt cactgtcgta
     8641 acagctacgc caagtggttt caattctaat cgcaaagcgt tggagaagcc aaggactgca
     8701 aatttcgtgg cagaataaac cgttgatttt gcagttgcca tttttccagc catagaggcg
     8761 atattaatga tatgaccttg tcttctttct gccatatcga tcgcaaattt ttgtgtcaaa
     8821 accatcattc ctaagatgtt gacttcaaac atatcgtatg cttttccaag gtcgaatgta
     8881 aggaagtctt cgaaaatccc aaaacccgca ttgtttacca gaatatcgac gcgcccgact
     8941 tcttccatca ccttttcata taattcgtcc acgctttcag gattgctgac gtctaccttg
     9001 aaagcataag cctctttgcc gctcaagcgt ctgcactcgt ctctgacacc ttctacaaat
     9061 gccattcttc gtgcgcaact tataacgatt gctccttgct ttgctgcttc ataacagatt
     9121 tgtgcgccca gaccgcctga actacctgtt actaaaacaa ctttatcttc taaattcatg
     9181 cctcttcctc ctcgcgaaat ggaatctcaa tgctatcaaa atctttcata atcgtcgtgt
     9241 tttcaaacac ttcttgtgct tctttttcaa gctctaaagc tgcttttcct aaatatctcg
     9301 cactgatatg agtaagtagc agccgttttg cctgcgcttc ttttgcaact tcagcagctt
     9361 gatgagtcgt ggaatggaaa tagttgtgcg ccattcttgc ttcatcttta ttaaatgtgc
     9421 tttcatgtac cagaatatcg ctattttccg caagcacaac ggaattatgt gtttttctcg
     9481 tatcgcctaa aatagtcacg attcgtcctt ttttatctgg acctacgaaa tcttttccat
     9541 tgatcgtccg cccatcttct agttggatcg tttcaccttg cttcagtttg ccgtacagtg
     9601 gtccagctgg aatacctaat tcttttagac gatccacctg caattctcct ttatgatcat
     9661 gttcaaccac acgataacca aaactgtcga tcccatgatt tagttttttt gtataaacag
     9721 aaaattgctg atcagtaaaa atcggggctg tttcatttag ctcaatgaat ttcagcggat
     9781 aacttaaacg cgtttgcgaa atacctaaag agacttttat gtattcctct atcccttttg
     9841 gcccgtaaat ctctaatggc gtatccccac cttgaaatga ccggctgctg atgagcccgg
     9901 gaagtccaaa aatatgatcg ccgtgtaaat gtgtaatgaa aattttaccg atttttctcg
     9961 gacgaatagt cgttcgcaaa atctgcattt gcgtgccttc gccacagtcg aacaaccaaa
    10021 cttcattgcg ctcgtctaaa agtttcaatg ctatacttgt cacatttcta tgttttgcag
    10081 gaacacctgc acctgttcct aaaaattgta attccataat ctacctgact ttctttaagt
    10141 tcctctttaa ttttagggta aaaagaaggg ataatcaacc gtaagtcgag aatgggttca
    10201 aattaaaaat aacgttctaa agcgatagcg ttttctttga aaattactat aaaaacagca
    10261 gcgaacccgt ttgagatcct ctgctgtttt ttattaattt gaagatccag aactttcatt
    10321 gtcgttggaa gtttgcggat gtttgtcctt ccaagtcttt tgtgcagctt ctttcgcatg
    10381 attttcagct tctttcgcat catgcacatc tttttcgaca tcttcataat ccaagcgagt
    10441 aatttctccg cctaattcca tcattaccaa ttgattcagt gggcgattgt cctcttcatc
    10501 agcaatcaag atcagtcctg tagctccttc accgatttta tttcctacaa agtcgaacac
    10561 ggtctgggca tcttttattt ctttggcatc ttgtgtcgca ccgatcattc ctccggcaaa
    10621 ccatcctaat aaaatcccta gcggaccacc taatacacca accaacattc cgattaatcc
    10681 acccgtagaa gatttgttat taccggtaaa gtcgataaaa tcttctattt tgaattgatg
    10741 tgtcccatta ggatcatggc tgacaactgc catctgttct cctttaatcg aacgaacagc
    10801 atgtaacttt ttgatttctg aaaatgcctg atatgcctgg ctttctactt gaaaatttaa
    10861 aatgattact cgtttgctca ttaaaaacgc ccctttcata ccttcttgtt atttatttta
    10921 cctttaattg aaaaataaga aaagaaaagc actctttttt tccaataaaa tagggctgtg
    10981 acaaaagtcg tatcacagct cctttttccg aataaacggc gttcttgaag ctgatcactt
    11041 ctgtcacaac ctcccttaaa tctgaatcta tattttttgg tatttgtttt tatttgcttg
    11101 cctattcgac aaattcaaat tcaaactcac cgatacgtac aaggtcgcca tctttcgcac
    11161 cgcgtgcccg aagagcttca tccacaccca taccacgtaa ttgacgagca aaacgcatca
    11221 ctgtttcatc atgatcgaaa ttcgtcattt ggaataattt ttcgatcttc tcaccagata
    11281 agatccacgt tgcatctgaa tcacgatcga tttggaactc aggaccttct ggattgaatc
    11341 catagtgtac agtttcttct tccagttctt cttcgtacaa tgggaattct ggcgttactt
    11401 ctaacaaatc tgctgtagca ttcaataacg cgtccaatcc ttgtcgagtc actccagaaa
    11461 ttggaaatac tgggatgtca tcagcaaatt catcttcttt ttccttgttc agctgttctt
    11521 tgaatttcac tagattttcc ggtgcatccg gcatatccat tttgtttgcc acgatgattt
    11581 gcggacgctc tagcaagcgt aagttataag tagataattc tttgttgatg gcaagatagt
    11641 cttcataagg atctcgtcct tccatcccac tcatatcgat cacatgtaaa attactcgtg
    11701 ttctttcgat atgacgaagg aattgtgtac ccagcccaac accttgcgaa gctccttcaa
    11761 tcaagcctgg caagtctgct gcggcaaaac ttctgccatc acttgtagta accattccta
    11821 gattaggtac gagcgtcgtg aaatgataag cgccgatttt tggacgagca gatgaaataa
    11881 cggatagtaa agtcgatttc ccgacagatg gaaaacctac aagtccaacg tctgccaata
    11941 ctttcaattc tagttcgatt tttcgctctt gtcctggttc accgttttca gcaatttccg
    12001 gtgcaggatt ccgaggagag gcaaaacgga tattccctcg tccaccgcgt ccaccttttg
    12061 ccacaactag tgtttgacca ttttcaatta aatctcctaa aagagcacca gtctccgcat
    12121 ctcggacagt ggttccttgt ggtactttaa cataggtatc ttctgaacca cgaccgtgca
    12181 tccctttact catcccattt tcacctggct gcgctttgaa atggcgattg aaccgaaaat
    12241 ccatcaatgt acgtaaccct tcatcgacga tcaagatgac atcgccgcca cgaccaccgt
    12301 ctccgccagc cggtccgccg tcaggaacgt atttttcgcg acgaaatgca accattccgt
    12361 ctccgccctt gccagctttc acatcgatcg ttacttgatc taaaaacatg gacataatta
    12421 agtcctccta ttattcattt atttatttga ctgcctgaat ttccacaata aacaaaagtc
    12481 ttacttattt tatcggtgaa atctgatttt gtctatcatt tcagcaaaaa gattgtttac
    12541 cttcttcttt ttttatcatt tggtaaggtt tccaaccttt tttctgctac caagtataat
    12601 tttttgacaa aatattcttt gcgtagcaga ataacagtgt attaattgta ttaattgagg
    12661 atgccgtttc acagcacccc taataaagga ggaacttttg atatgacagt aaaagtaggt
    12721 attaacggat ttgggcgtat tggccgtctg gctttccgcc gcatcaaaga agtctcagat
    12781 gatattgaag tagtagcaat caacgactta acaagtccaa ctatgttagc acaattattg
    12841 caatttgact ctactcatgg tacttatcct ggcacagtta ctgcaacaga agactcaatc
    12901 gtagtggatg gcgaagcaac tcgtgtttat gcagaacccg acgcaagcaa aatcccttgg
    12961 gcaaaagaaa acggtgtaga tatcgtacta gaatgtactg gtttttatac atctgaagaa
    13021 aaagcaaaag cgcatttaga tgctggtgtc aaacgagtag ttatttctgc cccagctggt
    13081 gctatgaaaa ccatcgtcta taacgtcaat gatgatacat tagatgaaaa cgacaaaatc
    13141 atctcagcag gttcatgtac aacaaactgt ttagcaccta tggcttattt cttaaataat
    13201 gaatttggga tcgaagttgg tacaatgaca acagttcatg cctacacttc cacacaaatg
    13261 ctcttagatg gacctgtaaa aggcggtaat ttgcgtgctg ctcgttcagc tgctgacaat
    13321 actatcccac attctactgg tgctgcgaaa gcaatcggct tagttatccc tgagctacaa
    13381 ggaaaattac aaggacacgc acaacgtgtt ccagtcgttg atggttcatt aactgaattg
    13441 gtttcagtat tgaaaacaaa agtaactgct gatcaagtaa acgaagcaat caaaaaacac
    13501 acagttgaca atccttcatt cgggtatgat gaccgtcaaa tcgtttctag cgatgtcatt
    13561 ggaacaactc aaggttcgat ctttgaccca acgcaaacag aagtaacaac tgctggtgat
    13621 ttccaacttg ttaaaactgt tgcatggtat gacaatgagt atggatttac ttgccaaatg
    13681 attcgcttac tagaaaaatt cgctaacttg taatttgata gatcaatcga taaaagaagc
    13741 ctttgactgg atccagtcaa aggcttcttt cttttcatcc cctactttta aactcgttca
    13801 atgaacatat tggcttaaaa attgttggac tacttgctga tatttttcag gatctgtctt
    13861 gtatgcttcc gcatgctcgg ctccttttac aacatacttt tcttttggtc ccttcgttgc
    13921 acgatagacc ttatctaaca tttcaaatgg tacaaatgta tcttcgtctc catgtataaa
    13981 gagcattggg cgctggtttt tcttcaattg ttccactgca tttgcttcac cgaaaaagta
    14041 gccagcacgg acttttgtca ctagactcgt tacttgcata agcggaaatg ctggaagact
    14101 aaacatatcg tctagctgat aagcaagctc atcatgaacg gaagaataac cgcaatcttc
    14161 aatgattgct ttcacattat ctggtagttt ttctccgcta gtcatcataa ctgttgctgc
    14221 tcccatgctg acaccgtata gaacgatttc ttgcgatttt ccattgtttt ctagcacttt
    14281 gtttatccac tgtacatagt ctttacgctc tggccatcca aatccaatgt aatccccttc
    14341 gctttttcca tgtcctcttg catcaggaac aaggacattg taacccatat catgaaacat
    14401 ctttgcgtaa ttggtcatcg tttcagcgtt tcccatatag ccatgagcca caagagctgt
    14461 tttttcactt ttcgtttcag caggaagata aatcgctgat aatttcagac cgtcttttga
    14521 agtgatggtc caatcgcttc ggttttcttt tgaactaaac catttatgtg catccgtttc
    14581 tttggatgtc tgtgttgtac caggtgtatc ttcgtctaaa aagtctttct cagaaggcac
    14641 aacagcataa ttataaaaat aattccctgc aaacactaac gcaacaatca gagctaaagc
    14701 gacgcctaaa aggattttca tccaaagttt cataaatcat ccatctcatt tctctttttt
    14761 catataacct tgaattagta taatcaaaga atctctacta tgcaatgttc atttttataa
    14821 c
//
LOCUS       DAZKKW010000065        14815 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.066, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000065 DAZKKW010000000
VERSION     DAZKKW010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14815
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.066"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(296..1024)
                     /locus_tag="ACG8PO_001960"
     CDS             complement(296..1024)
                     /locus_tag="ACG8PO_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0207266.1"
                     /translation="MLRIAIVDDDCTIVEGIRHYLLKKVPDIIIDHCYTDGDEAFKNL
                     QNQRFDLLITDITMGKISGLELIESLQQTSNRYFTSIVISAHQNFQYAKKGIDLSIKS
                     YITKPIDFPYLVKLVQEEKMRKSAANQNSLTDDSFNESIQQVIDYVQNNYSEKLTLKD
                     VATQFHYHPAYLGRAFKKETGYSFHDYLLDFRIKRASALLKDTFLSIDEISQKVGIDH
                     VSYFSVLFKKTTGHSPSNYRKKLL"
     gene            complement(1002..2576)
                     /locus_tag="ACG8PO_001961"
     CDS             complement(1002..2576)
                     /locus_tag="ACG8PO_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFQ0207267.1"
                     /translation="MKNDLSKVIMRFTYILIFFITLLACFTLFFSYINVIEKEKNQNL
                     QNIQQITHYMETYNRILSGIVTEIYQNYDFQENLNDYYLSSPAEYMEDILSKDRSPTY
                     LVDNLKKLVQDDEVKNISISLDYEKYLSAYAPTKNEKISFIRSLSFISEDMPDATLIL
                     DVSINALKKSLFPTHYNYSLSNGIDSVNLSEDISPSKWKKTQQIAIGNHTLQVYENPN
                     YSSQTLLGLAIMIFLLMSLFILAIHQLLNRSFRNYIQQYQEIITSLNHSSTGDYKSIN
                     IENKVGDLKEIAMHINQFYLDNATYQKTLYENNWLKQEAKYAALQYQIEPHFLFNNLE
                     FIRMTAILNDDEEISDSIYLLSKLYRNNTYQPDIIDLKEELINIDLSLQLHQRRYRHN
                     FDYQLIDHIDQLMLPKLSLQPFVENYIKHGFDSSKKENRLTVYATETTNHYVLKIVEN
                     GKGMDQDKLEHIQQKLNDDIPYSENIGISNSFRRIKHLYPGDTRFVIESQLHVGTTIT
                     IKIQKGGNMHVENCNC"
     gene            2805..3743
                     /locus_tag="ACG8PO_001962"
     CDS             2805..3743
                     /locus_tag="ACG8PO_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0207268.1"
                     /translation="MKLKKGLLGNLKKNRALFIMLLPGALWLFIFCYIPMFGVVIAFK
                     DFRISSRGFFTSLFNSPWVGWENFKYLFASQDAWIITRNTVVYNVVGILLSTFCTVSL
                     AIILSQMANKRLLKKMQTFMIFPNFLSWVVISYFVYAFLSTDKGLINILLSNFGVEPI
                     NWYSEPKYWPFIIVFTSLWKGVGYGSIIYYASIMGIDTSYYEAALVDGASKWQQIRNV
                     VLPHLRSIIVIMLILSVGSIFRADFGLFYQLPRNSGALYDVTNVLDTYVYRGLMNSGD
                     IGMSTAAGLYQSSVGFVLILITNKIVNKLDPSSALF"
     gene            3755..4666
                     /locus_tag="ACG8PO_001963"
     CDS             3755..4666
                     /locus_tag="ACG8PO_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0207269.1"
                     /translation="MISKLKRQMYFSLPVERFFTILLILFTLSCIFPFIFVIVISLTD
                     EKALQLNGYELLPAQWSTDAYQYLIQDGGQLLRSLGVTIMITVIGTLITVFMTGTYAY
                     VLSRASFPYRKFFTFYLFFTMLFAGGMVPSYLVMTKMLGLKNTIWALILPLAFSPYNV
                     IILRTFFKKSIPESIIESAKMDGCSEFRVFFQIVLPLAIPGVATIGLFSSLGYWNDWF
                     NALLYIDTNKLIPLQYLLMKIQNSMEFLANNNDITLAQQQAIQNSLPQESTRMAMVVV
                     ATLPIAIVYPFFQKYFVQGLTIGGVKE"
     gene            4690..6162
                     /locus_tag="ACG8PO_001964"
     CDS             4690..6162
                     /locus_tag="ACG8PO_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFQ0207270.1"
                     /translation="MKKNMQKYLIGGASVLTLGLLGACGSSNGEANNETGTTKVTWYQ
                     VGEEPKDFDEVMTKVNEKLKEEINVELDMRLIPDGDYQQKLGVMINSGEEYDICFVNG
                     TDYVNYGNKGAFISLNDHLDKELKDYAAELNEGFIEGGAINGETYGLPVNNDIATTEY
                     WTFNKDLVDQYQLDVENVEGVETLAPVLEKFAEKNTDTNITPIGMTRTSVCIPYDNII
                     GGGVPLAVAFEGDTQTVVNTYETPEMMTYLKAMHEFYKKGYILKDAATVTTDPFRIDS
                     KNWFLGRNSSGYQPLAQKKLEALAGHGLILKPAGKAFKTTGFARVAMHAVSASSKHPE
                     AALKLLNYVNTNQEVADLMVYGIEGKHYDRNEDGSITLTNSDDYYQGAWALVNTYLVS
                     KTSPITEESKKLEEESKKFQNEAPMSPILGFSFDASDFRTEIASVQNIVSQYEVLLSS
                     GTVDPEQYVPELLEKLEKAGLDKIQASAQEQYDEWRKSNK"
     gene            6213..6824
                     /locus_tag="ACG8PO_001965"
     CDS             6213..6824
                     /locus_tag="ACG8PO_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFQ0207271.1"
                     /translation="MNENKWTKGFNWVFQLLLLNFTYFILICLGFGILGFFPATIALI
                     AGVVNRKKYGLQESVKFMSKIYKKNFVRYNLYCWSATLLLAGNITTIWLCWQQSAPLF
                     YLLSTLLSVITLFLVFAMLNWVVIKSLGLWMMGDRLIDYLLFTATMLPTVVMQGLVLG
                     IYFRILMILPVIFLFGGTAVTVILISKLTERALAKLPCQIQSH"
     gene            6854..8767
                     /locus_tag="ACG8PO_001966"
     CDS             6854..8767
                     /locus_tag="ACG8PO_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HFQ0207272.1"
                     /translation="MKLTDKQVANIAFIQQLEIPLTQKDEEQIDLIFTDTKANVRQIK
                     ITKNEQQRICLSYSHEDLLYRGLFICLPRFLAQGGEMPYIEETSIIETSYSLDASRNG
                     VMNKETLKKFISYLALMGYNTLYIYTEDTYEVEDLPYFGYLRGAYTKEEVNEIVEFAS
                     GFGIEVVPSIQTLAHLTQFLKWYAQQELMDDQNTLLIGEEKVYAAIEKMIIACKEMYQ
                     TERIHLGMDEAYQAGLGRYLSLYGYCDRTTLMIQHMDRVLSIVDKYKMKPMIWSDFIY
                     KLLDETGKDRYYNPDFKLKALAAKGLPAGLTYVHWDYGSDEVSSYEKVIDNHLKFCDI
                     DHYVLASGAHIWGRLAPNHGKTEAIVSAAVKASKNKGVRSVMLTTWGDEGQETEHWHS
                     LIGALKLADYVYSEEEWNDFEEIYDRLLGTGSYQLMSELRYFDEVDTITNNNLQMASV
                     SKLLLWQDPLVGIYDFHVAAHNKIATRTIGTYYHELAQKLDMNKNWNPKQPILTTIRQ
                     RYILLAEILAQKAELGIYLQHCCLVEDLAGLMELANRCEALANRVNELASIHEIIWYQ
                     TYKTYGWEVLEQRYAALAHRLKTVARRIKNHLQGQDALEELLEERLPFCQYQVPLEIS
                     GFNYRQSSVSGYN"
     gene            8895..11876
                     /locus_tag="ACG8PO_001967"
     CDS             8895..11876
                     /locus_tag="ACG8PO_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HFQ0207273.1"
                     /translation="MLSDYYQNLSVISVNTEPLRNYYIPYASKEEAKQELEREKTSQF
                     IDLNGTWDFQYYPSVQQLPENFFQEAFTESYDRISVPGVWQTEGFDLPQYINVSYPIP
                     FDPPYVPNDTPCGVYRRQFQIEQTDARFYLNLEGADSNCFVWINRNFVGYDQVSHSTS
                     EFDVTKYLQVGVNEIQILVMKWCDGTYFEDQDKWRMSGLFRPVYLLQRPTMHLTSYQL
                     RTLTDPKKGSGTLMVSFQRFTPIEVSLPISYELFHPDGRLIAKGECYSEQQINLDVED
                     CLFWTAETPKLYTLLLTCNGEYFKEKVGFRALTIKNRKLLLNGTPIKLYGVNYHDVNQ
                     QGTPVMTREQLRADLLLMKKLNVNTVRTAHYPKSPDFYELADEIGLYVINEADLECHG
                     VVNLYDTEANYQLIANDPTFEKPIMDRVIRSILPYQNRSSIIMWSMGNESGYGVNFEK
                     AQALARKLDDSRLIHNERSIEPVIDQVNDFSNLDVISQMYPPLEDVHRHCQRSDTEKP
                     FFMCEYSHAMGNGPGDLKTYFDLIQQYDSFLGGCVWEWTDHAINRGTLETPSYLYGGD
                     FGEKYHDGNFCVDGLLTPDRQLTVGALEYRNIHSPIQLIAVDEELQEVTLSNITAFTA
                     GSAFNLSLEWYIDGKKTVYEVMNPFILEPGKSCSLPITVPTTIHGAHTVAVSLSVYKN
                     DELYGQQQLFLKKEKMRLPRTAHHNDAPQISVKGTELIIKGESFIYIYQLAKAAFTSV
                     KINQKELLERPTQFSIWRAPTDNDRNLVNKWKAAGYAEMSLEIRKKDWKFVDDHLLIE
                     TSYRGVIPGLQPIFTMHIQLKINGAGVINQKVSVEKNPIFPDFPRFGMDWPLNDTFSK
                     VSYCGYGPYESYPDKHYAAMFGIYEMEIEEFDQPYIKPQEYGNRWQCDWVSIKNHQGS
                     TMTVYGNSNFSALPYSQAQLTEKSHHYELIKENRTWFNLSYKQNGIGSHSCGPYLEDR
                     FRFMEDTFDWNIVITFKGENR"
     gene            11873..13534
                     /locus_tag="ACG8PO_001968"
     CDS             11873..13534
                     /locus_tag="ACG8PO_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein O-GlcNAcase"
                     /protein_id="HFQ0207274.1"
                     /translation="MKETKINQVKKKLSRYQEVVYRNHCLKWSKNKLNVRFNGLFYRF
                     EQLEEVWKPVTNFQSCPVQLINDPKETYDLCITHLYNQHLKNDGFYLKVTNDNQLTIE
                     SANLRGFQYAMEALLESFFSVGDQLLLPELILKHEPSVKIRGIIEGFYGIPWTHEMRL
                     SLFSYMKDNQLNTFMYAPKDDELLRKKWRELYDAQELDKFKELLSAAEASNIDFWYLI
                     SPGNDIDITCEEDIQVLLKKLEQLIELGVFQFGLLMDDIDYQLKGAAKRRFREPAFAH
                     AYLVNRVEEYLSTRLSNHELVICPTEYDNRHGSRYLATLSQEIPEQLPFFWTGPSTLA
                     ASISTEELQEMASVYQRPMLIWDNIPVNDYLEDKELLFMSPYENRTPNLSKERYQVTG
                     VVSNPMAQLEASKFTINSMANYLWNCERFDPLETWTSVVEKVVGSKLQPAYLTLTNAF
                     PNHYTSSLLSDEELLLAKDPEWVTKEMAALVQAVKWIQQEQHTSRLRTELEPWLQAIT
                     ESWTFWQEWQLKKDPKEAEEWLAKKKTHRIGRDLVTAHLEQIIKS"
     gene            13574..14506
                     /locus_tag="ACG8PO_001969"
     CDS             13574..14506
                     /locus_tag="ACG8PO_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HFQ0207275.1"
                     /translation="MVKRLISANYSDVKKMTSKELKQSIKASEGRVIVCETVANTLPQ
                     SGEITNAEVASAFGADLILLNGFDCYRPAVFGLPNVTIEKVFDLQQTIENPIPKLKEL
                     VGRPVGINLEPIAPKTNMMSEKVEISQGRASSVETIQKAEELGVDFICFTGNPGTGVT
                     NLEITKSVKMAKKYFSGLIIAGKMHSAGSEEPVVNKEAIISYAEAGADILLVPSVGSI
                     PGFLEQDLIEAVRLAKKYDLLTMSAIGTSQESARPETLRQLAIMNKSCGVDLQHIGDA
                     GYGGLAPAENIFETSVAIRGMRHTINCVSRSINR"
BASE COUNT         4815 a         2332 c         2978 g         4690 t
ORIGIN      
        1 atttaaataa ctacatccat ttattctatc ttctaaaata gcctttttca tttcagttaa
       61 atagttcagc gccatttttg cactcgactt gcatagtagt tttttccata ttcctcaacc
      121 tcagcggggg ttgaagacat aaggaaaatc ctatgcgtta tgctcaactg gatctgatta
      181 aatattctaa tgtatttttc tctaccatca ttaaatggtt aatagccaaa aatcaccact
      241 tttcaatggc tccttgttgt ataagtataa ctagaagatg aacacgtctg agaaattata
      301 gaagcttttt acggtaatta ctggggctgt gtccagtagt ttttttgaag agaacagaaa
      361 aatagctcac gtgatctatt cctacttttt gactaatttc atcaatagat aagaatgtgt
      421 ctttcagtag agcacttgct cttttgattc gaaagtctag caagtaatca tgaaaggaat
      481 aacctgtttc ttttttgaag gcccgtccta aataagcggg atggtaatgg aattgtgtag
      541 ctacatcttt gagtgtaagc ttttcactat aattgttttg aacgtaatca atcacttgtt
      601 gaattgactc attaaaagaa tcatcagtta gagagttttg attagctgct gatttacgca
      661 ttttttcttc ttgtactaac ttaactaaat aaggaaaatc aatgggtttc gttatgtaac
      721 ttttaataga taaatcaatc cccttttttg catactgaaa attttggtgg gcactaatga
      781 caatactagt aaagtagcga ttggatgttt gttgcaaaga ttcgattaat tcgagtccag
      841 aaattttccc catcgtaata tctgtgataa gtaaatcaaa gcgctggttt tgcaggtttt
      901 taaatgcttc gtctccgtct gtgtagcagt gatcgataat gatgtcgggg actttcttca
      961 aaagataatg ccttatgcct tctacaattg tacaatcatc gtcaacaatt gcaattctca
     1021 acatgcatat tccccccttt ttgtattttg atagtaatcg ttgttccgac gtgtagttgg
     1081 ctttctataa caaaacgtgt atctccagga tagagatgct ttatacgtcg aaaagaattt
     1141 gagatgccga tattctcact ataaggaata tcatcattaa gtttttgttg aatatgctcc
     1201 agcttgtctt ggtccattcc tttcccattt tcaacgattt ttaacacata atggttggtt
     1261 gtttcagttg catagacagt caaacgattt tccttttttg atgaatcgaa gccgtgttta
     1321 atataatttt caacaaatgg ctgtaaagaa agttttggta acattaattg atcaatatga
     1381 tcaatcagct gatagtcaaa gttatgtcga taacgacgtt gatgtagttg caaggagagg
     1441 tcgatattaa ttaattcctc tttcaaatca attatgtcgg gctgatatgt attattgcga
     1501 tacaacttgc tcaataaata tatactgtcg ctgatttctt cgtcatcatt caatatggca
     1561 gtcattcgaa taaattccaa attattaaat agaaaatgag gctcgatttg atattgaagt
     1621 gctgcatact ttgcttcttg cttcaaccaa ttattttcat aaagtgtttt ttgatatgta
     1681 gcattatcta aataaaactg attgatatgc atagcgattt ctttcaaatc accgactttg
     1741 ttttcgatat tgattgactt gtagtctcct gtagatgaat gatttaagga agtaatgatt
     1801 tcctgatact gctggatata gttacgaaaa cttctattta ataattgatg aatagcaaga
     1861 ataaacaaac tcattaataa gaaaatcatg attgccagac ctaataaggt ttgtgacgaa
     1921 taatttggat tttcatatac ttgtaatgtg tggttaccga tagctatctg ttgcgttttt
     1981 ttccatttag aaggcgagat gtcttcagat aaattgacgg aatcaatacc attagacaaa
     2041 gaataattgt aatgtgttgg aaagagactc ttttttagcg cgttgatcga aacgtctaga
     2101 atcaatgtag catcgggcat gtcctcacta atgaaactta atgaacgaat aaaagaaatt
     2161 ttttcgtttt ttgtaggtgc gtaggcactt aagtattttt cgtaatctaa tgagatagat
     2221 atatttttaa cttcatcatc ttgcacaagt ttttttaagt tatctactaa ataggtaggg
     2281 gagcgatcct ttgacaaaat atcttccatg tattcagcag gtgatgagag gtagtagtca
     2341 ttaaggtttt cttggaaatc ataattttga tagatctctg tcacgatacc ggagagaata
     2401 cgattatatg tttccatata gtgggtgatt tgctgaatgt tttgcaggtt ttgatttttt
     2461 tctttttcaa tcacattgat ataggaaaag aacaatgtga aacaggcaag taaagtaata
     2521 aaaaaaatca atatgtatgt aaaacgcata ataacttttg ataaatcgtt tttcattcaa
     2581 agtcctcatt ttgataatct ctaattttat gaatcattat agcacatatt acaagtgatg
     2641 taaacgcttt gcaaacaggg ctacttatga ttgtgaatac agtttccgtt taaaaactat
     2701 tctattgtag aggtttaata aactaaaaat aatgtataaa aaattaatgt aagcgattaa
     2761 ccaagtgggt ataatcaagg aaacgctgag agaggagaga caaaatgaag ctaaagaaag
     2821 ggttattagg aaatctaaaa aaaaaccgag cattgtttat tatgctttta ccaggtgcgt
     2881 tatggttatt tatattttgc tatataccaa tgtttggagt tgtaatcgct tttaaagatt
     2941 tcagaatttc atcaagggga ttctttacaa gtttgttcaa cagtccttgg gttggctggg
     3001 aaaatttcaa atatctcttt gcttcgcaag atgcttggat cattactcgc aatacggttg
     3061 tttataacgt tgtaggtatt ttgctaagta ctttttgtac agtgtcatta gcaatcattc
     3121 tgagccaaat ggcgaacaaa cggttattga aaaagatgca gacatttatg atttttccta
     3181 attttttatc atgggtagtc atcagttact ttgtttacgc atttttgagt acggataaag
     3241 gattaattaa tattttacta agcaattttg gtgtggaacc cataaattgg tatagtgaac
     3301 cgaagtactg gccttttatt attgttttta cgagtttatg gaaaggcgta ggctatggat
     3361 ccatcattta ttacgcgtca attatgggga tcgatactag ttattatgaa gctgctttag
     3421 ttgatggtgc cagtaaatgg caacagataa ggaatgtagt cttgccacat ttaagatcga
     3481 ttattgtcat tatgttgatt ttgtctgttg gtagtatttt tcgagctgat tttggattgt
     3541 tttatcaatt gccaagaaat tcgggggctc tttatgatgt gacgaatgta ttagatactt
     3601 atgtctaccg tggattaatg aatagcggag atatcggaat gtctacggca gcgggattat
     3661 atcagtcatc agttggcttt gtcttgattt tgattactaa taagatagtc aacaagttag
     3721 atcctagttc agcactattt taggaggaat caaaatgata tcaaaattaa aacggcagat
     3781 gtacttttcg ctgccagtcg aacggttttt cacaatatta ctaatcttat ttactcttag
     3841 ctgtattttt ccctttattt tcgtaatcgt catttcatta acagatgaaa aagcattaca
     3901 actcaacggt tacgaattat tgccggctca atggagcact gatgcatatc aatacttaat
     3961 acaagatggc ggacagttac ttcggtcatt aggcgttaca attatgatta cagtgattgg
     4021 aacgttgatt actgttttta tgacgggaac ctatgcttat gttttatcaa gagcctcttt
     4081 cccttaccgg aagttcttta ctttttatct tttttttacc atgttatttg cgggagggat
     4141 ggttccttct tacttagtga tgacgaagat gcttggctta aaaaatacaa tttgggcttt
     4201 gattctacct cttgctttta gcccatacaa tgtcattatt ttaaggacat tctttaagaa
     4261 gtcgattcca gaatcgatta tcgaatccgc taaaatggat ggctgtagtg agtttcgagt
     4321 ttttttccaa atcgttttgc ctttagcgat cccaggggtt gcaacaatag gtttattttc
     4381 aagcttaggt tattggaatg actggttcaa cgcattgctg tatattgaca ccaacaaatt
     4441 gattccattg caatatttgt taatgaagat tcaaaattca atggaattct tagcaaataa
     4501 taatgacatt acattagcgc agcaacaagc aattcaaaat agccttcctc aagaatcaac
     4561 acgaatggca atggtcgtag tagctacttt accgattgca attgtttatc cgttcttcca
     4621 aaaatacttt gttcaaggat tgacgatcgg tggagtgaaa gaataaaaaa gaataggaga
     4681 tgttaaaaga tgaaaaagaa catgcagaaa tatttgattg gtggtgcttc tgtactaacc
     4741 cttggcctat taggtgcttg tggtagcagt aatggcgaag caaataatga aacaggaacc
     4801 acaaaagtta cttggtatca ggttggagaa gaacccaaag attttgatga ggtcatgaca
     4861 aaagtcaacg aaaaattaaa ggaagaaatc aatgtagagt tggatatgcg gctgattccg
     4921 gacggggatt atcagcaaaa gcttggtgtg atgatcaatt caggagaaga atatgatatt
     4981 tgtttcgtca atggcacaga ttatgtgaat tatgggaaca aaggtgcttt cattagtctc
     5041 aacgatcatt tggataaaga gttgaaagat tatgcagctg aattgaatga aggatttatt
     5101 gaaggtgggg caattaatgg cgaaacttat ggattgccag tcaataacga tattgccaca
     5161 acggaatatt ggacctttaa taaagatttg gtagatcaat accaattgga tgtagagaat
     5221 gttgaaggtg tagaaacatt ggctccagta ttagaaaagt ttgcagaaaa aaatactgat
     5281 acgaatatta cgccaatcgg tatgacccgt accagtgtct gtattcctta tgacaatatt
     5341 atcggtggtg gtgtaccgct agcggttgca tttgaaggcg atacacaaac agttgttaat
     5401 acttatgaga ctccagaaat gatgacttat ttgaaggcta tgcatgagtt ttataaaaaa
     5461 ggatatattt tgaaagatgc agccactgtt acgacagatc catttcgaat tgatagtaaa
     5521 aactggtttt taggtcgtaa ctcaagtggt tatcaaccat tagcacaaaa aaaattagaa
     5581 gctctagcgg gacatggatt aattttaaaa ccagctggaa aagcttttaa aacgactgga
     5641 tttgcaagag tggcaatgca tgcggtatct gcttcaagta aacatccgga agcagccttg
     5701 aaattgttga attatgtgaa taccaatcaa gaagtagcgg atttaatggt ttatggaatt
     5761 gaaggaaaac actatgatcg caatgaagat ggttcgatta ctttaacaaa ctctgatgat
     5821 tattatcaag gcgcttgggc attggtaaat acttatctag tttcaaaaac ttcaccgatt
     5881 acagaagaaa gtaaaaaact agaagaagaa agtaaaaagt ttcaaaatga agcaccgatg
     5941 tctcctattt taggtttctc ttttgacgct tctgattttc gaacagaaat tgcaagtgta
     6001 caaaatatcg tgtcacaata tgaagtactt ttaagctcgg gaacagttga ccctgaacaa
     6061 tatgtaccag agttgttaga aaaactagaa aaagctggat tggataaaat tcaggcatct
     6121 gctcaagaac aatacgatga atggcgaaaa tctaacaaat aattttttac taaatgaaaa
     6181 gaatagacta agcagttagt ggggggattc aaatgaacga aaacaaatgg accaaaggat
     6241 ttaattgggt ttttcaattg ttgttactaa actttactta ttttatacta atttgtttag
     6301 gatttggaat tttaggtttt ttccctgcta cgatagcttt gattgctggc gttgtgaatc
     6361 gaaaaaaata tggcttgcaa gagtctgtga agttcatgag taaaatctat aagaaaaatt
     6421 ttgttagata taatctatat tgctggtcag caactttgtt gttggccggc aatattacta
     6481 cgatctggct ttgttggcag caaagcgcac cattgtttta tttattaagt acattattat
     6541 cggtaattac actgttccta gtctttgcaa tgttgaattg ggtcgtgatc aaaagtcttg
     6601 ggttgtggat gatgggagac cgactgattg actatttgct tttcacagca acgatgttgc
     6661 caacggtagt catgcaaggc ttggtgcttg gaatttattt ccgaatattg atgattttgc
     6721 ctgtgatttt tctgtttggc ggaacagctg taacggtaat attgattagt aaattaactg
     6781 aaagagcgtt agcaaagttg ccttgtcaga tacaatcgca ctaatttttt cacaaaaagg
     6841 agtatttctt tctatgaagt taactgataa acaagtagct aatatagcct ttatccagca
     6901 acttgagatt ccattgacac aaaaagatga agaacaaata gacttgattt ttactgacac
     6961 aaaggctaat gtgcgacaaa tcaagattac taaaaatgag cagcaacgaa tttgcttgtc
     7021 ttattcacat gaagacttgc tgtatcgagg attgtttatc tgccttccac gttttttagc
     7081 tcaaggagga gagatgccat acattgaaga aacctcgatc attgagacca gttattcatt
     7141 agatgcttct cgtaatggcg tgatgaataa agagactcta aaaaaattca tttcttactt
     7201 agcgttgatg gggtacaaca cattgtatat ttacacagaa gatacctatg aagtagaaga
     7261 ccttccatat tttggttacc ttcgcggagc gtatacaaaa gaagaagtga atgaaattgt
     7321 tgaatttgct tctggttttg gcatagaagt agttccatcg atccagaccc ttgctcactt
     7381 gacccaattt ttgaagtggt atgctcaaca agaactgatg gatgatcaaa atactctact
     7441 gatcggtgaa gaaaaggttt atgcggcaat cgaaaagatg attattgcct gcaaagaaat
     7501 gtatcagaca gagcgtattc atttgggaat ggatgaagcc tatcaagcag gtctgggacg
     7561 atatttaagc ctgtatgggt attgtgatcg gacaacttta atgattcaac atatggaccg
     7621 tgtgttatca attgttgata aatataagat gaaaccaatg atttggagtg atttcattta
     7681 taagctatta gatgaaaccg gtaaagatag gtattacaat cctgatttta aactcaaagc
     7741 attagcagct aaaggattac cagcaggatt aacctatgtt cattgggact atggttcaga
     7801 tgaagtttcc tcctatgaga aagtaattga taaccatttg aaattttgtg acattgatca
     7861 ttacgtttta gctagtggtg cccatatctg ggggagacta gccccgaatc atgggaaaac
     7921 agaagcaatt gtttctgcag ctgtaaaagc ttctaaaaat aaaggtgtac gttctgtgat
     7981 gttaactact tggggagatg aagggcagga aacagagcat tggcattcct taattggggc
     8041 attaaaatta gcagattatg tctattctga agaagagtgg aatgattttg aggaaatcta
     8101 cgatcgttta ttaggaacag gtagttatca attaatgtcc gaattacgat atttcgatga
     8161 agtggatacc attacaaata acaacttaca aatggcttcc gtgagtaaac tgctactttg
     8221 gcaagatccg ttagttggta tatatgactt tcatgtagcc gctcataaca aaattgccac
     8281 tagaacaatt ggtacttatt atcatgagtt agctcagaaa ttagatatga ataaaaattg
     8341 gaatccaaaa caaccaattt taacaactat tcgtcaacga tatatacttt tagctgaaat
     8401 tttagcacaa aaggcagaac taggcatcta tttacaacac tgctgtctgg tagaagacct
     8461 tgcaggatta atggaattag cgaatcggtg tgaagcacta gccaatcgag taaatgaatt
     8521 ggcatctatt catgaaataa tttggtatca aacatacaaa acctatggtt gggaagtatt
     8581 agaacaacga tatgctgcat tagctcatcg tctgaaaacc gttgcgcgtc gtattaaaaa
     8641 tcatcttcag gggcaagatg ccctagagga gcttttggaa gaacgattac cgttttgtca
     8701 atatcaagtg ccgcttgaaa tttctggctt taattatcgg caaagctcgg tgtcaggcta
     8761 caattaacga aggaatcttc ccgttgaacg gaccacttgc tcatctaatg ggaaagtggt
     8821 tcgttttcta ttcataagta gcttaccact aactctttta ttaagaagag aaaaaaagta
     8881 ggaagtgaga aaatatgtta tcggactatt atcaaaatct gtcagttata agtgtcaata
     8941 cagaaccatt acgtaattat tatattcctt atgcaagtaa agaagaagca aaacaagagt
     9001 tagaaaggga aaaaacgagc cagtttatcg atctcaatgg tacatgggat tttcagtatt
     9061 atccttctgt ccaacagtta ccggaaaatt tttttcaaga ggcctttact gaaagctatg
     9121 atagaatttc tgttcctgga gtttggcaaa cagagggatt tgacttacct cagtatatca
     9181 atgtatctta tccaattcct tttgatccgc cttatgtacc taatgatacc ccttgtggtg
     9241 tatatcggcg tcagtttcaa attgagcaaa cagatgcacg cttttactta aatctagaag
     9301 gagcagatag taattgtttt gtttggataa atagaaactt tgtaggctat gaccaagtga
     9361 gtcatagtac atctgaattt gatgtgacca agtatttaca ggttggggtt aatgaaattc
     9421 aaattctagt gatgaagtgg tgtgatggta cctactttga agatcaggat aagtggcgaa
     9481 tgagcggtct atttcgtcca gtgtatcttc ttcaacgacc aactatgcat ctgacaagtt
     9541 atcaactacg gacgttgact gatcctaaaa aaggaagcgg aacattaatg gtttcttttc
     9601 aacgatttac tcctattgaa gtatcgttac ctatttctta cgaattattc catcctgatg
     9661 gacgtctcat agcgaaaggg gagtgttact cggaacaaca aataaatttg gatgttgaag
     9721 attgcctttt ttggacagca gaaacgccta aattatatac attactttta acatgtaatg
     9781 gtgaatattt taaagaaaaa gttggctttc gagcactgac gatcaagaat cgtaagttat
     9841 tgctgaacgg aacacctatt aaattatatg gagtcaacta tcatgacgtg aatcagcaag
     9901 gcacgccagt gatgacccga gagcagttac gtgcagattt gttattaatg aaaaaattaa
     9961 atgtgaatac tgtacgaacg gcacattacc ctaaatcacc agatttttat gaattggcgg
    10021 atgagattgg tctgtatgta atcaatgaag cagatttgga gtgtcatggt gtggttaatc
    10081 tatatgatac tgaagcgaat tatcaactaa ttgctaatga tccgacattc gaaaagccga
    10141 ttatggaccg agtgatccgt tctattcttc cttatcaaaa tcgaagctct atcattatgt
    10201 ggtctatggg gaatgaatct ggttatggag taaattttga aaaagctcaa gctttagcac
    10261 ggaaattgga tgattcacgg ttgattcata atgaacgctc gattgaacca gttattgacc
    10321 aagtgaatga ttttagtaac ttggatgtta ttagtcaaat gtatccccca ctggaagatg
    10381 ttcatcgtca ttgtcaaaga agtgatactg aaaaaccttt ttttatgtgt gaatatagcc
    10441 acgctatggg caacggtcct ggagatctta aaacgtactt cgatttgatt caacagtatg
    10501 attcctttct tggcggttgt gtctgggagt ggactgatca tgcaatcaat agaggaactt
    10561 tagaaacgcc atcttattta tacggtggtg attttggtga aaaatatcac gacggcaatt
    10621 tttgcgtaga tggtttattg accccagatc ggcaattaac agtaggtgca ttggagtacc
    10681 gtaatattca ttcgcctatt caactgattg ctgtagatga ggagctgcaa gaagtaacct
    10741 tgagcaatat aactgctttt acagccggtt cagcatttaa cctttctctt gaatggtata
    10801 tagatggaaa aaaaacagtt tatgaagtaa tgaatccttt catacttgag cctggcaaaa
    10861 gttgttcatt gcccatcaca gtacctacaa cgattcatgg tgctcatacg gttgctgttt
    10921 cgctatctgt ttataaaaat gatgagcttt atggacagca acagctcttt cttaaaaaag
    10981 aaaaaatgag attgccaaga actgctcatc ataatgatgc accacaaatt tcagtaaaag
    11041 gcacagagct gataatcaaa ggagaatcct ttatttatat atatcaatta gcaaaagctg
    11101 cgttcacttc tgtaaagatc aatcaaaagg aacttttgga acgtcctact caattttcaa
    11161 tttggcgtgc accaacggat aatgatcgta acttagtaaa caaatggaaa gcagctggat
    11221 atgctgaaat gtctttagaa atcagaaaaa aagactggaa attcgtagat gatcatctcc
    11281 tgatcgaaac gtcttatcga ggagttattc caggattaca gccaattttt acaatgcaca
    11341 tacagttgaa aataaatggt gcaggcgtaa tcaaccaaaa agtatcagtg gagaaaaatc
    11401 cgatttttcc agatttccct cgctttggga tggactggcc gttgaatgat actttttcga
    11461 aggtttctta ttgtggctat gggccgtatg aaagttatcc tgataagcac tatgctgcga
    11521 tgtttggtat ttatgagatg gagattgagg aatttgatca gccatatatc aaaccacaag
    11581 aatatggtaa ccgctggcaa tgtgattggg tatcaatcaa aaatcatcaa ggatctacca
    11641 tgacggttta tggaaactct aatttttctg ctctgcctta tagtcaagct caattaaccg
    11701 aaaagtcaca ccattatgaa ttaataaaag aaaatagaac ttggttcaat ttaagttata
    11761 aacaaaatgg tatcggctca cacagttgcg gtccttattt agaagatcgt ttccgtttta
    11821 tggaagatac ttttgactgg aatatagtga ttactttcaa aggagaaaat agatgaaaga
    11881 aacaaagatc aatcaagtca aaaaaaaatt atctcgttat caagaagttg tttatcgaaa
    11941 ccattgctta aaatggtcaa aaaataagct gaatgttcgt tttaatggat tattttatcg
    12001 tttcgaacag ttagaagaag tctggaaacc ggtaacgaat ttccaatcat gtcctgtaca
    12061 gctcataaat gatccaaaag aaacatacga tttatgtata acacaccttt ataaccagca
    12121 tttgaaaaat gacggatttt atttaaaggt tactaatgac aatcaactta cgattgaatc
    12181 agcaaacctt cgagggtttc aatatgctat ggaagcctta ttagagagtt ttttttcggt
    12241 aggagatcaa ttattattac cggaattgat tctgaaacat gaaccatcag tgaaaattcg
    12301 aggaatcatt gaagggtttt atggtatccc ctggacacat gaaatgcgcc tctctctttt
    12361 ttcatatatg aaagataacc aattgaatac ttttatgtat gcccctaaag atgatgaatt
    12421 attacgtaaa aaatggcgtg aactctacga tgcgcaagaa ttagacaaat ttaaggagct
    12481 tttatctgct gctgaggcta gcaatatcga tttttggtac ttaattagtc ctggaaatga
    12541 tattgatatt acgtgtgaag aagacattca agttcttctg aaaaagttag aacaactgat
    12601 tgagcttggc gtatttcaat ttggtctatt aatggatgat attgactatc aattaaaagg
    12661 tgctgctaaa cgccgttttc gagagccagc attcgctcat gcctatttag tgaatcgagt
    12721 agaggaatat cttagcactc ggttgtccaa tcatgaatta gtcatttgtc ctacagaata
    12781 tgataatcgt catggttctc gctatttagc aacgttaagt caagaaatac cagaacaatt
    12841 accgttcttt tggactggcc caagcacgtt agcggcgtca atttctacag aagagctgca
    12901 ggaaatggct tctgtctatc aacggccgat gctgatttgg gataatattc cggtaaatga
    12961 ttatttagag gataaagaac tattgttcat gagtccatat gagaatcgaa caccgaatct
    13021 ttctaaggag agataccagg ttacaggagt agtttcgaat ccaatggctc aattagaagc
    13081 ttcaaagttt acgatcaata gtatggctaa ttacttatgg aactgtgagc gttttgatcc
    13141 tttggaaact tggacgtcag tcgttgaaaa agtagttgga agtaaactac aaccagctta
    13201 tcttacctta accaatgctt ttccaaatca ttacacaagc agtctgcttt cagatgaaga
    13261 attactgcta gccaaagacc cagaatgggt cacaaaagaa atggctgctt tagtccaagc
    13321 agtaaaatgg atccagcaag aacaacatac atctagattg agaacagaat tggagccttg
    13381 gttacaagca atcacagaaa gttggacttt ctggcaagaa tggcaattaa aaaaagatcc
    13441 aaaagaagca gaagaatggt tggctaagaa aaaaacacac cgcataggac gtgatttagt
    13501 aactgcccat ttagagcaaa ttatcaaaag ttagatatct taaaaaataa aaagcaagaa
    13561 aaaggagaac aaaatggtca agagattaat tagtgcaaac tacagtgatg tcaagaaaat
    13621 gacgtcaaaa gagttgaagc aatccatcaa agcgagtgaa ggacgggtga ttgtttgtga
    13681 aacggttgct aatactcttc cacaatctgg agaaattacg aatgcagaag ttgcgtcagc
    13741 atttggtgca gatttgattc ttttaaatgg tttcgattgt tatcgtcctg cagtttttgg
    13801 actaccaaat gtgacgattg agaaggtttt tgatctacag caaacgattg aaaacccgat
    13861 tcctaaatta aaagaactag tcggtcgtcc tgtgggaatt aatttagaac ccattgcccc
    13921 taaaacaaat atgatgagtg agaaagtaga aatcagccaa ggaagagcaa gctctgttga
    13981 aacgatacaa aaagcagaag aactaggagt cgattttatt tgctttaccg gtaatccagg
    14041 cactggagta accaatttag aaattactaa atccgtaaaa atggcaaaaa agtatttttc
    14101 gggattgatt attgctggga aaatgcatag cgccggctca gaagagccag ttgtgaataa
    14161 agaggcgatt atttcttatg cggaagcagg cgcagatatt ttactagttc cttcagtagg
    14221 gtctatccca ggctttttag agcaagacct aattgaagcg gttcggttgg caaaaaaata
    14281 tgatttgtta acgatgtctg caatcggtac tagtcaggaa agtgcccgac cagaaacatt
    14341 gcggcaatta gccattatga ataaaagttg tggtgttgat ttacaacata ttggagatgc
    14401 tggatacggg ggattggcac cagcagaaaa tattttcgaa acctcggttg caattagagg
    14461 aatgcgacat accatcaact gcgttagccg ctcgatcaat cgctaacaaa attaaataaa
    14521 aaatggttaa attgattatt taagtgggaa catacttgag atatcagcta acttctgtca
    14581 ctttaaaaaa gggactgtga cagaagtgaa aatagacaaa aaatatccga acaagagact
    14641 aggatagcag aaaaactatc gttttgcaac gaggaccaac tggaccgtag gagcaattct
    14701 cttattgttc ggatatttcg cttgttcaag gacttatgtc tgtagtgccc ttttttggta
    14761 ggaaaaatgg tagggaacca tatcattttt aatgaaaatc aagcatttca aaagt
//
LOCUS       DAZKKW010000066        14283 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.067, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000066 DAZKKW010000000
VERSION     DAZKKW010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14283
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.067"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..888
                     /locus_tag="ACG8PO_001970"
     CDS             <1..888
                     /locus_tag="ACG8PO_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HFQ0207276.1"
                     /translation="RNLDSRTRFEQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYY
                     WVKKLDKPDKYSKIKQEITAIVKESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMG
                     LTCQIRIKRYKSYKGTVGKIAKNVLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPIL
                     DLFNGEIISYSISTSPTYKLIEEMLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLK
                     ENNIIQSMSRKGNCLDNSVIENFFGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKR
                     IKLKLNGLSPVEYRKQSIK"
     gene            complement(928..1626)
                     /locus_tag="ACG8PO_001971"
                     /pseudo
     CDS             complement(928..1626)
                     /locus_tag="ACG8PO_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
     gene            1705..1881
                     /locus_tag="ACG8PO_001972"
                     /pseudo
     CDS             1705..1881
                     /locus_tag="ACG8PO_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348541.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
     gene            complement(2057..2626)
                     /locus_tag="ACG8PO_001973"
     CDS             complement(2057..2626)
                     /locus_tag="ACG8PO_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFQ0207277.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(2700..3023)
                     /locus_tag="ACG8PO_001974"
     CDS             complement(2700..3023)
                     /locus_tag="ACG8PO_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0207278.1"
                     /translation="MLLLKTLQERGYLDVHPPEVKIVFFFRYEHQEARRLAGVLNKTL
                     RHRKRISIHVCTRNKPPRYADLVIIDHFFPLDHNEDQKTYLYHPSFGIERLLTDINYW
                     LGKNR"
     gene            complement(3177..3963)
                     /locus_tag="ACG8PO_001975"
                     /pseudo
     CDS             complement(3177..3963)
                     /locus_tag="ACG8PO_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274430.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            4290..5165
                     /locus_tag="ACG8PO_001976"
     CDS             4290..5165
                     /locus_tag="ACG8PO_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010758089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0207279.1"
                     /translation="MGKRSTIQLMIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGT
                     FSRVQCTPDLLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLE
                     AMAENHATIDNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDD
                     ELLLLLDKFEKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAI
                     QLGISPRGSLSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKK
                     QIIHTLVAQIPIPVR"
     gene            5179..5502
                     /locus_tag="ACG8PO_001977"
     CDS             5179..5502
                     /locus_tag="ACG8PO_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0207280.1"
                     /translation="MEKIKDYFINFVLFLVYFIIVLFALVFNNSLGWFLLAFFTLYVS
                     WSLISLSSFLKKLSVTCTLQPLVYRKKSMQVIFSIAKKRTFSLPLPRLQIGFPAIFAE
                     KNKRF"
     gene            5499..6251
                     /locus_tag="ACG8PO_001978"
     CDS             5499..6251
                     /locus_tag="ACG8PO_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HFQ0207281.1"
                     /translation="MILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFRKSAKR
                     EISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNRIDWKL
                     SAHTQEWLYREYDYQEEECSPLFCFWGSPSPKFEPLLDLYFSLWHLRKEKQPELLILG
                     NRAFHGKDPGHTYFASLEAGDEKTFFAHLKKYAKKQIVLFVPENSPEVSEAIETLSKD
                     RTVYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            6241..8412
                     /locus_tag="ACG8PO_001979"
                     /pseudo
     CDS             6241..8412
                     /locus_tag="ACG8PO_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
     gene            complement(8524..10695)
                     /gene="copB"
                     /locus_tag="ACG8PO_001980"
     CDS             complement(8524..10695)
                     /gene="copB"
                     /locus_tag="ACG8PO_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HFQ0207282.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIR"
     gene            complement(10715..12901)
                     /locus_tag="ACG8PO_001981"
     CDS             complement(10715..12901)
                     /locus_tag="ACG8PO_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFQ0207283.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGLIAVADQIKLEVKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLCLNRHMKK"
     gene            complement(12901..13110)
                     /gene="copZ"
                     /locus_tag="ACG8PO_001982"
     CDS             complement(12901..13110)
                     /gene="copZ"
                     /locus_tag="ACG8PO_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HFQ0207284.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(13123..13563)
                     /locus_tag="ACG8PO_001983"
     CDS             complement(13123..13563)
                     /locus_tag="ACG8PO_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HFQ0207285.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYYPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(13637..14176)
                     /locus_tag="ACG8PO_001984"
     CDS             complement(13637..14176)
                     /locus_tag="ACG8PO_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFQ0207286.1"
                     /translation="MKIIVIGQSGSGKSTLARKIKEITDFPLLPLDLLWHTTDYSIQA
                     KKWFLQEQQLFMVSNPSWIVEGNYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLK
                     RKINKKSRPDMPESFSEKLNREYLEFLSFIWHFEENNEPKIQQLIQVANARNKLIILK
                     TRREKQEFLTKLKIESKNN"
BASE COUNT         4150 a         2920 c         2524 g         4689 t
ORIGIN      
        1 agaaaccttg attcaagaac aagatttgag caagaagaaa tcaaggttaa aacaatcctt
       61 cagctcaaaa aagaatttaa attgaatcta ttactttcaa ttgcacaatt agcgaagtca
      121 acttattatt actgggtaaa aaaattagat aagccagata aatatagcaa gataaagcaa
      181 gaaattacag cgattgtaaa agaatcaaga aactcttatg gttatcgtag agtcacttta
      241 gcgttaaaga tgaaaggata tacaatcaat cataaaacag ttagaaaatt aatgtcccaa
      301 atgggactta cctgtcaaat ccgaataaag cgatataaat cttataaagg aacagtagga
      361 aaaattgcca agaatgtgtt aaaacgaaat ttttcagtag atacacccaa taaaaaatgg
      421 gtgacagacg tcactgaatt caagataaaa ggaagaaaaa tctatctatc tcctatactt
      481 gatttattta acggagaaat aattagttat agtatatcaa ctagtccaac atataaactg
      541 attgaagaaa tgcttcaaca agcaattaaa aaaaagggaa ctgagggttc actgattcta
      601 cattcagatc aaggatggca gtatcagatg ccacaatatc aaaaaaaatt aaaagagaat
      661 aacattattc aaagcatgtc tagaaaagga aattgcttag ataattctgt aatagaaaat
      721 ttttttggtg tgcttaaatc agagtttttt tatcgagaaa aattccgatc aattgagata
      781 tttcaaagta aattaaatga gtatattagg tggtataata acaaaaggat aaaactaaag
      841 ttaaatggac tatctccagt agaatatcgg aaacagtcca ttaaatagtt caacttttgg
      901 ggttcagttc aaagaggggg ggatttttta tcgttgtgga atatttaaat accaacgctt
      961 gtcatgaaaa tcttgtgctc cacccttagt atttccttct ggatcgtttg tcgcacgcat
     1021 catgacgtat actttcttat tagggaaatc acgcatattg aaagatacat gatagccaac
     1081 atttcctaaa gtattataag cttgatttac gtctggtcta taaatcccat cagctcttac
     1141 tctagctagt tcttttccag tattgtagtc cataatgaaa atatactcgt atttataatt
     1201 agcaatgtgc catccagcta catgcaagtt tgcgttttcg atttctccga actgatcaat
     1261 gtgggcgtaa tttgttccat ctgtcagcgt agaatttgca gcacctgctc gagttggatc
     1321 aattactggt ttatcctccg aagtagttgg attatcatcc gtaaaaccat gagccaaatc
     1381 ataggctagt ttttctttac ttacacctat ttcagaaaga taaccgtaag gatctgtatg
     1441 atcgccccaa atattttgtg ttacccataa atgcgatttg attcctggtt ggttataagg
     1501 agtgtctaat gttaatggaa taccatattt cattgctgaa tctcttgcca attcaacgta
     1561 tactttatag ttcttttcaa aagtttcttt atcatgtgtg tgttgtaact cgatttgcac
     1621 aggacttttg gctcacgagg ttgtcctact ctacatcttg ctgatagata tgctgaaagg
     1681 aaaacaccaa cattatgttt tgctttttca gctagaacga gacaagcagg aataccttta
     1741 gaagatatca aaggtttttt aggtcataaa gatgtttcca ctacacaagt ctatgcccat
     1801 atttctcccg aagtgaaaaa aagatcaatg aatcaactag aaaactatat agaagagcaa
     1861 ataaaaaagc actcaaattg agtgcttttt taagctcact atcactaaaa ctatcactaa
     1921 ttttatttat aagcgaacat gtttctatca ctgatttttt aaatctagcg aaaaaccctt
     1981 atcttaaagg cgttcgttca attctttggc aaggtcttca aagcctggct tgcctagaag
     2041 tgcgaacatg gttttcttat agctcatgta gaaaaatttt cttgatgatg attttatcca
     2101 tcaattgaga ttgactgttt tgctctccta tgatttcagc tatgattgtc cgattttgcg
     2161 gttttcttct taactggtag ttgattttcc cagtttccat caactcgcta agcatatcca
     2221 cgactccgcc aaatccggtc tttttcgtaa taaaacctag tgctaccatt cgttcatcta
     2281 gattttttgc tgtaatcgtc acaccagtaa tgagcgagtg ctgtaatgtt tgggccagct
     2341 cttccatccc ttcagcattg gatacgacgg aagctgtttt gttaataggt tcttcgttta
     2401 tttcttttgg ttctttcctc tccactttca ctgtttgacg aaggacatct agttcttgca
     2461 agagcgtttc gtattgttct tgggtcagca tcacgccagc tactttttca cgattaaata
     2521 tatatacgcc agttcccgcg tctcgcgctt gagcaaatac atccatcggc gatcgtttca
     2581 cttctgtgat cgaagatgtg ggaacttcta atttctttaa ttccaatgga tttcctcctt
     2641 tactaatcgc tttttattat agcacagact gacttgtttt gatctttttt ccaaaggttt
     2701 tatcggtttt tgcctaacca gtaattgata tcggtaagaa gtctttcgat accaaatgaa
     2761 ggatggtaca gatacgtttt ttgatcttcg ttatggtcta gtggaaaaaa atgatcgatg
     2821 atcactagat ctgcataccg aggcggctta tttcgagtac atacatgtat agagatccgt
     2881 tttcgatgtc ttagcgtttt gttcagtacc ccagcaagtc ttcttgcttc ttggtgttca
     2941 tagcgaaaaa aaaagacgat tttcacttca ggagggtgga catctaaata ccctcgttcc
     3001 tggagtgttt tcagtaaaag catcccccac tctactgcgg ggtgtgcaag tgcttgttgg
     3061 atcgctagcc gaaattgata tagtttatct ttggaattgg gaaatttttc taatgtttcc
     3121 tgataataac gaacttcttc tatcagccgg tcatggatac caattacgta acgaagtcat
     3181 tcttggtaaa gtacttttgt cagctttgat ttgctttcgc tctcaaattc gctattcagc
     3241 caaaacagcg aatcaagctt ttcagtcaca atcgtcaatt tccgatagtc ttgtgtctct
     3301 tctatccgtt tccaccgctt ttcagtaatc tggttacagc tcaagcgcaa aattgctaga
     3361 taattttttt cttttattgg caaaatctgc tcctctctaa atagcaattc aggaaacaat
     3421 tcaaagaata gtgttcgact gtctcctaaa atctcgattt ctgccggatc ctttctttgc
     3481 ttcaataatt ggatgaacat ctcagaaaag gactgctctc catcatctgc aaaaaaatgc
     3541 ggaaaatctt tcaaaaagca aaacagcaga tatcttactt ttctttctga accaaagatc
     3601 tgtgcttttt tataagaaaa agaaaggtgg tactcgccaa accactttct caatcgcttc
     3661 gtaaggttca ttcaatagca gatttttcag taaacaaaac gggggtgatt ggatcagaaa
     3721 aacaaacaag agataaggtt cgctatatcc ttgatcataa actaactgaa cggttttctt
     3781 atgaagaatc agctgaatgg gacagtcgct ttccgccaaa cgtttcagat gccgatccac
     3841 tgttttaaca tcgattttca gttcttcagc taaatcagct tttcgccaga tcaaatcttt
     3901 cattagattg attagtgaaa ataaattctg tgcatttttt gataatagtt tttgccatct
     3961 catcgatcat ttctcacctg atttctaaac atattgtttg atcaaaatgg gttcactcct
     4021 aaaaagataa aaaactccct ttctatttat ttaaatacgc aaatcttcct cattattttt
     4081 tattttactc aatttaatcg tattctcatt tcaaaacgaa tgtactttaa aaaaacataa
     4141 taagatcact ttgcttgtag gatgtattcc cttccattac aatgaaataa atagcgattt
     4201 tatgagaagg tgatgtgaaa atggaagaaa aaatagaaga atccaaacaa aaaatcgatc
     4261 agttgatata agaattagaa aaagcggtca tcggcaaacg ttcaactatc caattgatga
     4321 tcgtggccct attagcaggt ggtcatgtct tattcgaaga tattcccggt gtaggtaaaa
     4381 cgttactggt caaagcacta gcaaaagctg tacagggaac cttcagcaga gtccaatgta
     4441 ctcctgatct gctgcctagt gatattcttg gtttttccgt atacaattca caatcaaaag
     4501 agtttgaatt tcgtccggga ccgatcttta ccacgatctt gttagcagat gagatcaatc
     4561 gtacgactcc tcgtactcag tcagcacttt tagaagcaat ggccgagaat catgcaacga
     4621 tcgacaacaa tacataccct ctagacaatc atttttttgt cttagctacg caaaatccaa
     4681 tcgagtacga aggaacatat cctttgccgg aagcacagct cgatcgtttt ttattccgac
     4741 tacaaatcgg ttatccttct tttgatgacg aacttttgct acttttggat aaattcgaaa
     4801 aagtgttgga taacttaaat gaagtattga acagttatga aatagaagaa ataaaaacaa
     4861 atgtggcgaa tgtctttgtc agtcccgaag tggcatctta tgccttgcag ttagtgtcag
     4921 caacaagaag tcatgctgcc atccagcttg gcatcagtcc acgaggaagc ctttccttta
     4981 ttcaggcagc aaaagcttat gctttaatcc atgggagaaa ttacgtcacg cccaaagatt
     5041 tgcaggattt agttccttac gtgttttccc atcgcctgat tttttacgat cgttcattga
     5101 aggaagacga aaagaaacaa atcattcata ctcttgttgc acaaattcct attcctgtga
     5161 ggtagattga ggtagacaat ggaaaaaatc aaagattatt tcattaattt tgtgttattt
     5221 ctggtatatt tcatcattgt gctttttgct cttgtattca acaattcttt aggctggttc
     5281 ttgttggcct tttttactct ttatgtatca tggagcttga tttcattaag cagctttcta
     5341 aagaaacttt cggttacttg tacattgcag ccactcgttt atcgcaaaaa aagtatgcag
     5401 gttatttttt ccatagcaaa aaagagaact ttttctttac ctctccctcg tttacaaatc
     5461 ggcttcccag caatctttgc agaaaaaaac aagagatttt gattcttaca aataaaccgc
     5521 aagaagtcca attgctttgg cagccaaaag aaagaggatg ccatgaaaca ttaactgtca
     5581 tttttacagc ctatgatagt ctaggacttt tcagaaaaag tgcaaaaaga gagatctctt
     5641 ttcctgtgac tgttctgcct gctttttgta agattcaagc agaaaaatta cagagagttc
     5701 tggaaaaaaa acagcagctg aacgcgtacc aaagaggtct tgattttcgg gaacatcgcg
     5761 cttatcgtcc aggtgattct ttcaaccgaa tcgattggaa attgtctgct catactcaag
     5821 agtggttata ccgtgaatac gattatcaag aagaagaatg ttctcctctt ttttgctttt
     5881 ggggttcgcc cagtccaaaa tttgaacctc tgctagactt gtatttttct ttatggcatt
     5941 tgagaaaaga aaagcagccg gaacttctga tattaggcaa ccgcgccttt catggcaaag
     6001 accccggtca tacctatttt gcttctttgg aagctggcga tgagaaaact tttttcgcac
     6061 acttgaaaaa atatgcaaaa aaacaaatcg tcctctttgt tcctgagaat tctcctgaag
     6121 tctcagaagc tattgaaaca ttgtcaaaag atcgaacggt ttatcttgtt tatttcgtag
     6181 aaaaacaact catggtccaa gaaaaagaca agatatgttc attgccagga ggtgaatgga
     6241 ttgacgatta aacgggaaaa atggctgcgt ggcgtttact cctttgtgat actaagtgta
     6301 ggtgctccga tatttgtagt agctaatcaa ttgcaaaaca gctaccttct tatcctaact
     6361 attggtgtcc tatgccttat tgtctgctgt attcctaaaa aatgggtacg tttgctgctt
     6421 tctttgccag taatgatagg ttgtttgttt ttatattttc cttcgaagac attttccata
     6481 ttatggatct tacagttttt ttcgtcagtt acagaggaat tcttccatct atcccaaggt
     6541 aaactttatt atttgccaga aaagacagct ttttttctca taatgttgct gatctattta
     6601 tttattacat tgaccgtgga ttacgactat tggtacgcac cctttctttc tttgatggct
     6661 tacttcatga tgcttaccgt ttttcggaaa aaagatttac tgtacgaaat gattgcggta
     6721 gtaactgttt tgttcgtgta tctggcagca aggcaatttt tctccatccg gttgttgtcg
     6781 agaaacactc gattccagtc attgactaca gttctgctta tttgtttctt aacttttagc
     6841 gctatcttgc ctttatatct tccgaaagta caccaatctc tcgaggaaac gagtgaaccg
     6901 atccgagaat atcttaatga tgaagggctt tatgacacac tccatgagta tcaattaacc
     6961 ggaagtgcaa gaacaggctt cagtgagaat gatgaacagc ttggtggacc tttatatgat
     7021 aatggcgaag ttgtttttac agcgaaccaa aaaggaaatg gctattggaa gatcgaaaat
     7081 aaaaatcatt acaccggttc tggatgaata gaagatcagg acgcacccat ccaaccaatc
     7141 caagaattcc ctgttgacat cacgagctac caaaccgtta tagaagacaa agaaaacatt
     7201 acattgaccc taaaaaaaca agctttctta ccttatcctt atggaaatac taccttgact
     7261 gatttttcat tagctagtgg acaaacgctt tactatttgc ctgaaagcca gcgattcatt
     7321 cctgtcccga atgaatcgac acgttctgca actttacaaa cagaaaggtt agtttttacg
     7381 agagaagaat taaaaagcag tcagttaaaa gctgatgatt tagaaaatca gcagacaaat
     7441 cttcaattgc caaaaagttt gcctaaacgt attgaggact tggcggtcga attgaccaaa
     7501 aatgaagcaa ctttaattcg taaagtcgaa gctgtctaac agtatttaaa aacaagcgga
     7561 aatttccgct attccaaaac ggataccggg catactccaa aagaacacga ttatgtggat
     7621 tatttcctct ttgattcaaa agtcggctat tgtgacaact tttcaacttc tatggtcgtc
     7681 cttcttcgaa cacttggtat cccagctcgc tggacaaaag gttttacaaa tgggctcaga
     7741 acaggcacag ataatcaagg gaatgaggaa tacagcattc tcaatagtca tgctcattct
     7801 tggccagagg tatactttcc tggatttggc tggcttccat ttgaaccaac cccttcattt
     7861 gccaatccaa tgggttcaca accagttgct gaaacaatcg aaaccgtcca gcctgctatc
     7921 gaacaaccaa atgaaagcga aaatatggca gaaactattt ccagtgagag atctgaggtc
     7981 attccatcag aagaaacaac actcacacca gcttataaaa tcgtcttatt cagccttgga
     8041 gtgtgtttat ccgtcttgtt gttctttcta ttgttattca gaaaatctct gcgctggaca
     8101 agttatctta tctatcttca tttttttgct tctactaata cgattgcata tcaaaaactt
     8161 cttaaccctt tggaaacatt ggtaccgcgc aaacccgcag aatctttgca ggaatatagc
     8221 ctgcgaatag aacaagaatt caagctagat ctgcgtttca ttcgattaac tactcaatat
     8281 gaagcagaaa tatatggacg acaagcagat ccggtaaaat tcgataaaaa aacctattta
     8341 gaaataacgc atataataag aaaaatggta tacaaaaaaa ttctgacaaa agggagccgc
     8401 ttcaaaaaat aagataaaat ttctggagaa tctacttctg gaatgccgag aaccagctga
     8461 ttgagtatcg attactcgaa aaaaaggttg tgacacgttt tttgtcacaa ccttttttca
     8521 ttattatctg attttcaatg tcaaagcatt tagagctaca actaccgtac ttaacgacat
     8581 caagacagca ccaactgctg gacttaatat gataccaatt ggcgcaagaa ttccggctgc
     8641 tagcggaatt gcaagaatat tgtaacctgc cccccaccag aggttctgga tcattttctt
     8701 tcgcgtttgc ttggctagat caagaaaatg caaaatatct tttggatcgc tgtttgtcaa
     8761 tacgacatcc gcagaatcga ttgcgatatc tgtccctgca ccgattgcca tcccgattga
     8821 agcacgggct aaacttggcg catcattgat cccatcaccc accatgatta cttttttccc
     8881 ttggtcaagg tatttctgga caacagcttc tttatcatct ggtaataaac cgccataata
     8941 ttcttcgatc cctaaataat ctgctactgc ttgggcagcg tttttattgt caccagtaag
     9001 catgacagga gtgatttttc tttctttcaa tgttcggata aattcttttg cttctgtttt
     9061 tactacgtca cctaaagcaa cgattgcagc tagatgattt tctaaaatca ggaagctgat
     9121 ggtatttcct tgttcttggt aaggtttcaa taatgcttgt tctacgttta atccgagacg
     9181 ctcaacttct ttttcattaa ctattttcac ttgttgtttt ttgactgttg cctctagccc
     9241 gactcctgaa agatttttta gattttcagc ttgataaggc ttgatttcat gttctttcaa
     9301 ataattcata atgccaacag ccaatggatg attggcattc gcttccaagg ctcctagata
     9361 ttgaaggatt tcatttttat tgaacgcatc atcaagtact tcgacacccg ttacagtaaa
     9421 ttttccttct gttagtgttc ctgttttgtc cagcatgatc acatctagat cattggcttg
     9481 ctccagtgca ttgcggtttt tcaaaagtaa tccgttttta gctgctattg atgtggaacg
     9541 agcaacaact aatgggatag ctaaacctag agcatgagga caggcgatga taaagactgt
     9601 taccatacgt tctaatgcat taggaagatc tgtcaagaac aaccaggcga tgaatgccag
     9661 gatacccaca attaatgcga cataaaacag ccattttgcc actttgtcag ataatgactc
     9721 tagcttggat ttttctcctt gtgcttttcg caccatttcc ataaccttcg ctagataacc
     9781 attttcacca gttccagtta cttcgatttc gatcgttcca tccccattga tagaaccgcc
     9841 aatcactttg tcattggctt gttttttgat tcctcttgat tcgccagtca cagctgattc
     9901 gtcaacaatt gtttcacctt tcaagattac accatctgtt ggcattttat caccagaccg
     9961 gacgatcaaa cgatcacctt cgtttacttc tttcaatgaa actgtctctt cttttccatc
    10021 tttcgtcaag cgtttcactg attctggcag taattctgct agtttttgta aagcattgct
    10081 ggcattcgac acagcattca tttcaatcca gtgacctagt agcataataa cgatcaaagt
    10141 agccaattcc cagaagaaat ccatgacgtg tgtatgtgga ttgagcagat ttgcgataaa
    10201 cgaatagaca ctataaatat atgcaactgt aatccccatc gcgatcagtg tcatcatagc
    10261 aggacttttt tgtttcaatt ccatcttagc tccacttaaa aatggctgac cgccatagat
    10321 aaatagtata gttgctaaga tcaatacgag ccaatcagaa ccaggaaacg ttacttgaaa
    10381 tggaaattcc attcccatca ttggtgaaaa aagaatgatt gggatagcta ggataagcga
    10441 tagccaaaat ttttgtttga aattccccat gtgcatggaa tgatccatgc cgccgtgttc
    10501 gtgatccatg tgcatcgaat ggtccatttg tttgctgttt gctatatctc catggttatg
    10561 tcccatgtgc atggaatgat ccatctgact atggtcatgt ttcatctcca tctgtttttc
    10621 atgagattct gtactttctt tgtttactac ttctttttta tgctcatgtt ttttctccat
    10681 atcgttccca ttcattttta ctccatccct tctcttactt tttcatgtgt cgatttaagc
    10741 acagggagtt caacaataca ctgacggaac taaacgccat cgctcctcct gcaatgattg
    10801 gattcaaaaa gcctagtgcg gcaaaaggaa taccaatcgt attatagaca aaagcccaga
    10861 aaagattttg tttgattttt ctcaatgtca acctagacaa ctcgattgtt tgtgcaatgg
    10921 atgccaaact gctattcatc agcgtcacat cggctgtttc catcgcaatg tccgttccac
    10981 ttcccattgc cataccgaca tcagctaatg ctaaggccgg tgcatcattg attccatcgc
    11041 ctgccatacc gacttttttg ccatctttct gaagtttttc tacgtaggct gctttatcct
    11101 ctggaagtac ttcagcaaag atatgctttg gatcaatacc aacttgttta ccaatcgttt
    11161 cggctgccag tttgttgtct cctgtcagca taaaaacatc taatccttta ttctggagct
    11221 gcttgattgc ttgtttgact tctagtttga tttgatcagc cacggcaatc aacccgatca
    11281 cttgcttttc atcagctaaa aacatgactg tttttccctg ctgttctaat tctaacgcgt
    11341 gttcttggta ttcatcaaat gataggttca attcaattaa tcgtttacgt gtacccgcaa
    11401 aatagcgtac tccgttaatc gtaccgctga ttccagcacc tgggtgggct gtaaaatcaa
    11461 tcatatcgta tgttgctgct tcttgcagtt ttccgtattc cacgattgct ttacctagtg
    11521 gatgttcaga agcttgttct aaagtataaa aaatagatag gacttcttta gaaccgacta
    11581 gatctgtaac tttgggcttt ccttctgtaa tcgttccagt tttatccaaa acaatgctgt
    11641 ctaagtgagc agctgcttct aatgcttctc cgcctttaat taaaattcca tttcgtgcac
    11701 caacacccgt tcctaccata atagctgttg gagtcgctaa acctaatgca catggacacg
    11761 caatgactaa tacagagaca ctatggatca atgcgagttg ccaatcacca gtgatcaaac
    11821 ctgtagctat gagcgtgata aacgcgatga ctaatacgat cggaacaaaa atccctgaga
    11881 tccgatctgc gattttttgg attggggctt tgctcccttg agcatcttcc accatttgaa
    11941 tgatttgcgc taatactgtc tgcttgccga cttgtgtaac ttccatgtga agtagaccat
    12001 tcgtattgat tgtcccgcca aagagcgtgt catccggatt cttttctaca ggaagatttt
    12061 caccagtaag catactttca tctatcgcac tgcttcctga aataattcga ccatctgctg
    12121 gaatctgctc accaggacgg atgactagtt gatcaccaac gactacttct tcaatcgcta
    12181 acgtttcttc tttcccgttt cttatcactt gggcggtttt tgtttgaaga gacatcaatt
    12241 gtttgattgc attacctgtt ttcgtttttg ctgtatgttc caaatatttg ccgagcaaaa
    12301 tcagcgtgat gatcatactg ctgctttcaa aataaagatc ttgggggtgt ccacggaaaa
    12361 atccgttgta aatgctcaat gcaaaggcag ccgatgttcc aattgctacc aatacatcca
    12421 tatttggtgc tttcgtcttg attgcatggt acgcaccttt ataaaaacga gcaccgacat
    12481 aaaattggac aggagcagac agcactaact ggacgattgg aaaatgaaaa aagtgaacaa
    12541 tcgccgcatg gcttcctaac atcatagcaa tcattgacaa catcaacggc aaagtcaaaa
    12601 ccgtactcaa tataagatcg aatagcattt ttttcaaata cgcttgcttt tcttctgcaa
    12661 ttttttgttt gtgggcttca tcgtacaaaa ttgcaccata gccgatgttt tctacgcttt
    12721 ggattaattt ttcagtggtc gtcccttcat atttgacact tgctttttca gtggccaagt
    12781 tgactgtggc attgaccaca ccaggttgtc cgttcagctc tttttcgatc ctagcagaac
    12841 aatttgcaca agtcatacct gtaatgacga acgtatccat tttggtttct gctgttgcca
    12901 tcaaatcacc tctgctttat aacccaatcc attgattgct tgacaaatct tttcggattg
    12961 aacttttgtt tcatcaaact tgaccgtacc gtttgctttt ttcaaattta ctttcacttt
    13021 ttgcacgccg tcaagtgcgc ttactgtttc ttctactctt gctgcacaat gattgcagct
    13081 cataccttcg attgaaaatt gttgtttcat tttttcttcc tcctattgat gttgtttaca
    13141 ttcacattga cctgggatac agttacattc gatcgtatct actggttgct tttgttccag
    13201 ttgtttcaaa atctcattga tatcttcttg agtcaaagtg gcttcttcta ctagatccgc
    13261 aatcgtctct ccaatccttt ttgcacagat atgggaaaaa agattttccg tcgcactgcg
    13321 aactgtttcc atctccgaga cagctggata ataaatgaat ttttttcctt cttgttctgt
    13381 ccagatcact tcttttttta ccaaacgacc cagcaaagtt ttgatcgttg ctggtttcca
    13441 atccatcgaa tcagctaaga tctgcgtgat ttcttgggct gtcgtctgtc caagtgtcca
    13501 aataacacgc atgatctccc actctgaatc actgatcttt actggcgttt tgactttttc
    13561 catttatttc acacctttcg tttacaaatg taatcattat cttatgaata cagattacac
    13621 ttgtaaacaa aaactgtcaa ttatttttgc tctctatttt caatttagtt aaaaattctt
    13681 gtttctctcg tcttgtttta aggatgatca gtttatttct tgcattagct acttggatca
    13741 gctgctgtat tttcggttca ttgttttctt caaaatgcca gataaaagat aaaaattcta
    13801 agtattccct gtttagtttc tccgaaaatg attcaggcat atctggccga gattttttgt
    13861 ttatctttct ttttagagaa cgccaaacta ctcggtagat ggcgagataa cgcggcactt
    13921 taagccaaat gatcttatct gcttctttaa tcctttcctc taatgtagaa gtataatttc
    13981 cttcaacgat ccatgacgga ttagatacca taaacaattg ctgttcttgc aaaaaccact
    14041 tttttgcctg aatactgtag tctgttgtat gccataaaag gtctagcggc agtagcggaa
    14101 agtcggtgat ttctttgatc ttccttgcga gggtcgattt tcccgaccca ctttgtccaa
    14161 tcacgatgat cttcattctt ctccctcact tctctttgga atttggttcg atcataccac
    14221 ttataacgcc gcaaaaaaac aagggactgt gacaaaagtc gtgtcaaagt cccttgtttc
    14281 gaa
//
LOCUS       DAZKKW010000067        13818 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.068, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000067 DAZKKW010000000
VERSION     DAZKKW010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13818
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.068"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            155..349
                     /locus_tag="ACG8PO_001985"
     CDS             155..349
                     /locus_tag="ACG8PO_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0207287.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            618..917
                     /locus_tag="ACG8PO_001986"
     CDS             618..917
                     /locus_tag="ACG8PO_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0207288.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(1014..1448)
                     /locus_tag="ACG8PO_001987"
     CDS             complement(1014..1448)
                     /locus_tag="ACG8PO_001987"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0207289.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1680..2390)
                     /locus_tag="ACG8PO_001988"
     CDS             complement(1680..2390)
                     /locus_tag="ACG8PO_001988"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFQ0207290.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(2380..3240)
                     /locus_tag="ACG8PO_001989"
     CDS             complement(2380..3240)
                     /locus_tag="ACG8PO_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HFQ0207291.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(3245..3883)
                     /locus_tag="ACG8PO_001990"
     CDS             complement(3245..3883)
                     /locus_tag="ACG8PO_001990"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFQ0207292.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3898..4197)
                     /locus_tag="ACG8PO_001991"
     CDS             complement(3898..4197)
                     /locus_tag="ACG8PO_001991"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0207293.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKEVTVIGIKNVMSAKEISEKFAETKWYQKNK"
     gene            complement(4240..5724)
                     /locus_tag="ACG8PO_001992"
     CDS             complement(4240..5724)
                     /locus_tag="ACG8PO_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFQ0207294.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQVLIFMGLILGCYWAVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPEGYFLIVEDYDTYAEKQRV"
     gene            complement(5744..6205)
                     /locus_tag="ACG8PO_001993"
     CDS             complement(5744..6205)
                     /locus_tag="ACG8PO_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFQ0207295.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGEDEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(6223..7290)
                     /gene="ulaG"
                     /locus_tag="ACG8PO_001994"
     CDS             complement(6223..7290)
                     /gene="ulaG"
                     /locus_tag="ACG8PO_001994"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HFQ0207296.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADSKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            7508..8278
                     /locus_tag="ACG8PO_001995"
     CDS             7508..8278
                     /locus_tag="ACG8PO_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFQ0207297.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(8551..9378)
                     /locus_tag="ACG8PO_001996"
     CDS             complement(8551..9378)
                     /locus_tag="ACG8PO_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0207298.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
     gene            9945..11180
                     /locus_tag="ACG8PO_001997"
     CDS             9945..11180
                     /locus_tag="ACG8PO_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFQ0207299.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
     gene            complement(12179..12439)
                     /locus_tag="ACG8PO_001998"
     CDS             complement(12179..12439)
                     /locus_tag="ACG8PO_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFQ0207300.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(12466..13767)
                     /locus_tag="ACG8PO_001999"
     CDS             complement(12466..13767)
                     /locus_tag="ACG8PO_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFQ0207301.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEKAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNATLLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPMIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
BASE COUNT         4440 a         2828 c         2178 g         4372 t
ORIGIN      
        1 cttttattat atatattatt caaattagtt atatcgagtg aaaaaaactt tcaaattgat
       61 tctttttatt tatcataaag atatagatac ataatcattt tcacttacat atatttgaat
      121 attaactaaa aacagcaaga aagaagagga tcaaatgtat tatgtagaag taaaaacaaa
      181 aggcgtcaaa aacaaacaat atgtgaaagg tatcagtaac gaatatcctt tacttggttc
      241 atggaaagaa gctgctcctt tttccaaacc atgtgccata aaaataaaga acgagctaga
      301 aaaagaactg acttgtggca aagctgttgt agatatcatc gaaaagtaaa tccaatgtat
      361 agaaaggaag atcacacatg aaaagcgacg tacaaattgc taagattata gaattagaag
      421 aagaagacgc agtgttgaca attgaaaaaa aataaatgtt tgtataccat atgattattt
      481 caatatgtat cctggaattg gagatctcgt agagctatat caagatgaat caaatatact
      541 tgtcattcat catgaaatat agagacttat aaaattgttt atttttataa tgtatcagta
      601 tagaaaggaa ataaaacatg gattatactg ttgaatttga cagtgaacta cttaagcaat
      661 ttccacaaga agaacatttc atgtataaag aagcaatttc ccacaaagtg acagagtatt
      721 tggagcataa tttttttcgt ataaagccag tgcgtactcg ttcggcaaaa ggaatatacg
      781 agatgaaagt caaaattaat aaaaaaaatt atcgcatagc atttgcttta aaaaaaaatc
      841 atgtgttcat tttttatatt agttcgaatt tgcaaaaaat tgtatttgat aaagaggtga
      901 gtaaaaaaat aaaatagctg agcattggag gatagattga gtgatactaa gtctatcctc
      961 ttgtttttct aaagaactaa gctagaattt tttcttttgt acgttattat atgttatttg
     1021 tcattaccaa aaagcacctt ttttcttcta gtatctatga cataagaaat accacccatt
     1081 tcctttactt gaacaaatcc tactgattca taaaattttt ttgttttctc agtatcatct
     1141 gtcaataatt cgatttggac tggtccataa ctagtgatct tctccaaagc ttcattcaaa
     1201 agcactttgc ctattccttg gcgctgatac ctcggatgaa tgaggatatc ttgaataaat
     1261 gcgatcgtcg caaaatcagt gatccaacga acgattccga taatcttccc ttccccatta
     1321 cgctttatca gagattcaga ttgttcaaat gctctagcta accttgcagt gtcttttgtg
     1381 taattcgtcc aaccaacgct cttatataat tcagaaatct catcgatcat aagggaatca
     1441 atattcattt ttttcttcct ttcttataca attctccctc taataataca gttctttgac
     1501 tatttaatct atcacatata actctcctgt aaagattctc tcgattcctt aaaatctctc
     1561 tttcctttta gaatgcatcc tttttgataa ataaaaaggt tgtgataaat tttcttcaca
     1621 acctctgttt ctctctatca agtgttatct tttaattttc tacccttacc ctagcatctc
     1681 tattgcccgt agtatgcata ctttccatgt ttgcgaaaat aatgtttatc tagtaaatat
     1741 tgcgggattt cacatatatt ggggttgatc agctcgtttt ctttggccat cagacaaatt
     1801 tcatctaaga tcaaactgtt ttctactgct ttcataggtg aatcccccca tgtgaacggt
     1861 ccatgtccat agacaagtac tccagggact tcgtttggat ctaattttct ttccttaaac
     1921 gtctcgacaa tcacattccc tgtatgaact tcgtaagctt ctcttacttc ttctttcgtt
     1981 aattggcgtg tacaaggaac cttaccataa aaagcatcag catgagtcgt gccataagct
     2041 ggcaaatcgc gtcctgcttg cgcccacatg acggagtgcg tggaatgagt atgagtaata
     2101 gcagttatat cttcaaaagt ttgatacaaa acaacatgtg tggggagatc tgaagatggt
     2161 tttaagctat cttcttctat tatatttccc gacaaatctg ttaccaccat ttgatctgct
     2221 tgcatgcatt catatctgac accgcttggc ttaatgacga tgactcctag ctcacggtcc
     2281 acttcactca cattgcccca agtcaatttg actaatccgt atcgcggcaa tgccagattt
     2341 gcttcaagga cacgttgctt cattgctgca attttttctt cacaagacat catagccagc
     2401 ctccttcaat ttagaactta agtagtcttt tgcctgctgg atttctcttc gaaagtcttg
     2461 gcttttttca ctccacattt caatcagaaa tgttccatca taatctagac gtttcaaatg
     2521 tcttaataat ccaagaaaat caacacaacc gctaccaaaa ggaacatcac gaaactggcc
     2581 tgaatattct tttgttacag ggtaagtatc ttttaagtgg atagcagtga tataatcgat
     2641 ccctttctca agttctcgag ccggattatt ttccggccat gcggacaaat tgcctaggtc
     2701 tggatatact tgtagaaaag gggaatgaat ctgttctttg atttctaaaa attttgaaat
     2761 ggagttcata aatggatcat ccatgatttc cacggaaaga atgatgccat atttagaagc
     2821 ttcctttacg ctttctttta ttccttcgat aaagtcttct ctggaattca ttgatttctc
     2881 ttcataataa acatcgtatc cagctatttg gattactttg acgcttagat tatgcgccaa
     2941 atggattgct tttttcatta gttgtttcgc tgcttttttc ttttctggat cagcagaacc
     3001 aaacggaaat cgccgatgcg cacttaaaca aatcgaatgg atacgtacac cggagctgaa
     3061 catctcttct ctcagctcag ctatcttttc ttcagaccaa tctaatcgtg ctaaccgctc
     3121 atccgtctca tcaatcgaca tctcgataaa atcgaagtcc atttctttta caagggaaaa
     3181 acgttctttc catgaaatat ctttgggtaa tgctttttca taaatcccaa tttgagccat
     3241 ttccttaccc ccagatacga atgatctcat ctttgaagtt ttgtgcggcc ttcatcggat
     3301 catttgctgc tgtaatacca cgaccagtga taaaggtata cacatcgatc ccttcaaata
     3361 agtgtaatgt gtgtggattt aatccaccag ttacagaaac attaaagcct agttcaatca
     3421 gttttttgat cttcgataaa tctttttcac cccaagtttc accagataac agcgcatcac
     3481 ggctttgatg gtagattacc tgccggatac cgatgttatg ccaatccatg gcttgttcaa
     3541 aagtccaatt gccgtagagt tctacttgca attcacctat ttctttttgt gctgcctcca
     3601 tagttggaat cgttgcacaa caaatgactg tcatccagtc tgctcctgct tttgcaacat
     3661 tactcgccac tgttcctcca gcgtctgcac atttcgtatc agcgactagt tttttatctg
     3721 ggaacatact gcgaatacag cgaatcgctt tttgtccttc ctgtaagcaa agaattgtgc
     3781 ccacttcaat aatatctact acatcaccaa ctttcataac atctcctaat gcatgttcta
     3841 agtcattgtg atctaacgca atttgcaaat tcggcctgct catcttagtt ccttccttta
     3901 cttgtttttt tggtaccact ttgtttcagc aaatttttca ctgatttctt tggctgacat
     3961 cacatttttg ataccgataa ctgtaacttc ttttttctct gcctgagtaa acatattcaa
     4021 aaaattgact ggcgtaaaaa ctacatcgta ttggccagct gaactttttc cctctgaaat
     4081 ggcacaatga tggattttgg taattggtac gtttagttct ttcattgctt tttccacact
     4141 tctcatcatc atcaagcttg ttcctgatcc atttgcacac gatactaaaa ttctcatctt
     4201 aatccctcca ttatgagtgt tgtctaatca gattttcttt tatactctct gcttttctgc
     4261 atatgtgtca tagtcttcta caatcaaaaa ataaccctca ggatttttcc gatattgtaa
     4321 ctgcggaata gccaataaac caatgacaac tacggcaatt ccagcataac ctaggacttt
     4381 catgattact gtaaacaatg gccaagctgt tgcccagtcg aacatcccga tataaccacc
     4441 atatgttgac atcccaatcc atgtagcgaa taatgctgaa ccgcctactt gtatgattcc
     4501 tgaaagaaac gggaacaggc aagcggcttt gaatccacct cggttattgg cataaactgc
     4561 tataacggca ttatcaaaga acaacggaat gaaaccggct atgacgatcg ttggtgattt
     4621 caataaaatc agtaaaccaa tcatcaaaaa ctgtcccaaa gcaccaaaca agaacccaat
     4681 cgttaccgca tttggcgaac caaagccaaa agtagcagct acgtcaattc ctggaacagc
     4741 tcctggtaaa attgtgtttg agattccttg gaaagattga gttagttcat cgacgaatgt
     4801 ccgaacacct aattgcaaga tcgccagata taccgcaaaa tttaaagaag tcgtcataat
     4861 gtagaataag aaactctgtc cttcttgcat gaattgcgct tgaatcaaat aatctttgcc
     4921 tagtacaatc agaatgatcc caaagaaaaa tagcatgagt aacgaagtag caaccatatt
     4981 ctcattgaaa atcgacaaga atcctggtaa tttgatatct tcgatttttt tgtcttgctt
     5041 gattgatcta tttttctttt catcccgctt tttcatccac tcagctaatt ttgcaaatat
     5101 aaagattccg aacatttgct gatgtgccac tgcaaatccg gcaccttctg tcaattcctg
     5161 cgtgattcct actgttaagt tagaagctac tgcccaataa caacctaaaa tcagtcccat
     5221 aaaaatcaat acttgtatcc tacctagatc aggaaagcaa aacagtaaaa tccaaaaagc
     5281 ggtagctgct tgttgaacct gaacgtttcc ggtagtgaaa acggaacgca gtttcgtata
     5341 tttctgaaaa cggactaata agatatttaa aacaaatgca atcagtaata aaatcatcac
     5401 atctccaaag gtacggccaa atgtctccat taatccagca tccacagcat tttgtccaaa
     5461 ataaggatct gtaaccatgg catccaaatt gaaccgttcc tttagtccaa ccaaaatcgg
     5521 ccgaaaatta ttgaccaaac caccagagcc aacagtcaaa ataaagtaac cgaccgttgc
     5581 ctttaagaat ccagcgatgc attcatgaag cgggcgtttc agcaaaatat agcctaataa
     5641 cacgataaaa ccaatcaggt atgctggctg cgtcaagata tttgttgcaa aatacgtcca
     5701 aatgcttaac aaaaattccc ccatcttttt tctcctctat caattaatta tattctgcca
     5761 tcacaacttg gtaatcatcg atggagttcg tagagatgag acgctcaacc aatccatctg
     5821 tcattaataa ttcggataat ttttggatat tttctagatg ttcttctgga tcttttgctg
     5881 caagtgtaaa aaacaaacta gccgttttct cctcatcctc cccttcactt tcaaaacgga
     5941 ttggctgttt caacttggta aatgaaatag ctgttccaaa aacaccatca cttttttctg
     6001 aagaatgcgg cattgctact tgaggtgcga tcacaatata aggaccgtat ttttctacac
     6061 aatggatgat ctcctccaca tacgtttcat caataatttc cttatcgatc aatacaagac
     6121 agctttcggc gatagcttca cgccaatccg tgatttcttt atctgggtaa tgaatcaatt
     6181 gatggtcaaa aaagtatttc agcaagaaac tccccttctt tcttataaaa acgacggaaa
     6241 cggtgtatca ttttcaatcg agaagacatc ttcaaatcct cgataaaaat taaactcaag
     6301 atcatctttg tttgtcggat agatgaattt cccgcctact tgccagatat aaggtttaaa
     6361 tccatacttc aggcgatcct ttttgaattt ccaaatttca gtgatctctt ttgaatcagc
     6421 cataaaatta gaccaaatat cgtagtggac aggaatcact acttttgcat tcaatgactc
     6481 cgccatgcgc agcatatcaa cagaagtaac tttgtctgtt atccctcgtg ggttttcacc
     6541 ataggcccct aaacagacat cgattttgtg ttcattacca tgtttggcaa ataaattgga
     6601 gtaatgtgaa tctcctgcat gataaagatt ccctcctgat gtttcgaata aatagtttac
     6661 tgcaatttga tccatctcct gaggcatttt tccttttaat acctcttctg gatcttcaca
     6721 agtgaccaac gctgtccgat caaacgcttc taaagctact acattgatgt ctttgatttt
     6781 gactgcatca cctggcttta ctgtaatcgt tttttcttca ggaattcccc atttttgcca
     6841 cgtgcgaacc acttcttttg gcccaatgaa tgttgcttca ggacaatttt gatgaactgc
     6901 tgcggctgta tggatatcta gatgatcgga atggatatgt gtaacgatta aagcgtctac
     6961 attttttatc gcaaaaggat cgattacgaa aggctgagta cgtaaattag gctgcatatt
     7021 ttgaacgcca ctcatacgca tcatctgatg gccttttttc atcatgccat tcccatgact
     7081 acgttttccc gtcccacacc atagatcaca taaaatattc gttccttcat gacttttcag
     7141 ccaaattccc gtacagccca gccaccacat agcaaaagtt ccaggatcca ctacttctgt
     7201 ttcgatttct tcattcaagt atgtgcccca ctcaggaaat gtttctacta accacttttc
     7261 ttttgttact tcattgacag ttttactcat ttgcttgctc cccccatatc gttgttccaa
     7321 cttacaagta aatattaatg tgtatcagca tccatggaaa cccttttata tgaccgtttg
     7381 ttttttatat gattttcttt cagtaatcaa tgattgtttc agtataataa agacagatat
     7441 gaatgtttaa atgaacaact agtcaaataa atccatggat caagatgtcc ctcaggaggc
     7501 aaaagtcatg aaaaactcac ttgtcaatat tgaaaaacgt caaaaagaaa ttttggaact
     7561 gcttcagcag actgaacaac tatcaacttt cgaattagct gaatcccttg atgtatctat
     7621 tagtacgata cgaagagatt taaatttatt agaagaaaaa aacgagatca tacgcaatta
     7681 cggttattgt tcttataatt acaaaaacca gactgatttt gatcaatctg gtcctgaaag
     7741 gattaaacaa gcgattgcca gagaagctag taaatacatt aacgattatg atacgctgtt
     7801 tatcaattct agctcgactg cgttgaaaac actggatttt cttcaatcta accatttgac
     7861 agtcattaca aacaacgtga aaatcaatta ctctccgcac aaagataatt gtagttatat
     7921 tttaactggt ggagagatgc gctttccaaa agaagcacta gtgggcgaca tcgcattaaa
     7981 tacgatttct tcagtcaatg cagacatttg tatcattggc tgtagcggag tcgatgtagt
     8041 acatggggtc acaacaaaga tattaaatga atcaacgatc aacgaaatga tggtcaacaa
     8101 gacaatcaaa tgtaagattt tagtcgctga tcatcgaaaa atcggactga catcaaagtt
     8161 caagattgct gatatttcag tctttgatta tttgatcact gataaatttt gttctgaaaa
     8221 agtattagag gagatcagaa atacggggat aaaaaccatt caagtagatt cattttaaga
     8281 accaggacgt aaatatgtaa ataggattat caaaaaaatg aataacaaat ttatcgataa
     8341 atgaaaaaca cgctacgctc aaattgataa aggaatccca agatgtgtcc atccaatcga
     8401 taccatcata atctattgct tcttttataa ccgttatatg ttgcctaaat aggaatcaaa
     8461 caatatcgaa aaaaggaggt atgacaaaag tcacacctcc ttttttcgaa taaacggtat
     8521 tcaaatcaga acactgattt cacaaccact ttatttcagg tcgttttcaa tcacctttac
     8581 cgcgtaatca tttccgttga aacgcgctat tttttttgcg tccatatatc tttctaaata
     8641 atcatcttct cctaattgct tatggcaaga agcaatttca taaagcaagt attccaatgc
     8701 ataagtcgat ttatgtttct tattatactc aatacccttc tcacataatt ggattgcttt
     8761 atcataatcc tcgattttac ggtaataggt ggcgatatca taatatacaa tgcagaccat
     8821 cagattatca aaatcagtct cactcaatat tttaagtgta cgctccacat aaactttcgc
     8881 aaaatctaat tctccttttg acaagtagat cattgccacg tttgcagctg cttgtaagtg
     8941 atggatatct gtttcggtcg tttgttccaa cacttgatta aaataaaata atgctgcatc
     9001 tggttttttc gcataaagat gatcaagcag tccttgataa aataaaattt gatttaaaag
     9061 atcagaatta cttacttttt cctgatcgat cgtttgtaaa attttataag attcttggta
     9121 ttttgataca gaattcaatg attgaatctc atttattttt ttcctgattt tgtcttcttc
     9181 tgtttctact aatatatcgg agatctcgac attcagcttt ttgcaaagcg cacttaagat
     9241 attgatgcct ggcgacatat tcttgttttc gatattgctg acagttgctt gttcacaaat
     9301 gccttctgct aattgtactt gtgttaaccc ctttttcttt cggtattctt ttagtaatgc
     9361 tccatctact ttaatcaaaa caatttcctt ccctttaaaa aattatttct cctttatatc
     9421 atacacaaaa aaaagttcta aacaaccaaa aatgtttaga acagaataaa aattttaatt
     9481 ttaaaataat attcaaatta aacaactggc gtatctgctc cttgatttct ttttgtgtta
     9541 tctgaataat tatcgtttac agttataaca catagtgaaa attgtcatgg cgagaaagtc
     9601 taagaatatt tttaaacata actatttctt tcttacgtgt gtataatgac gacagcacac
     9661 ctatcaatta atataattat tttatttttt atgttctcat gtttattttt aatgacacaa
     9721 cttctcgtat ttatctggca caacatataa atcgatcaaa aaattgttgc gtgttttttt
     9781 gaaaattgga gtttcaactt gattacaaaa tttaaattta gaagttgtga aaacagcgag
     9841 agacagttta atattcaaaa tatacatagg ctactcgaac gctgtgtttt ttagtatact
     9901 agtaagaaac gaagggatag actcattcca ataaggagga tttcatgaag acaattcttt
     9961 ttgctcctgc agtattcaat ttagctgaga caacacgaat gattgaggtt gccaaagcta
    10021 tgaaagaccg ttattactgt gtattctttg gcttttctaa aacttttgct cctttgatca
    10081 cagaaagtgg ctttgagtat catttactag cgccgatcct tacgaaaaaa caagaagaac
    10141 aaatcataaa atttgaccag ttgcgtacac ttaaaaaccc ttttaccaaa gaaatggttg
    10201 cctctcgtat cactagtgaa cagagtctga ttgctaagtt tgagcctcga ctgattgtga
    10261 caggttcgaa tgtgacaatt tttttatcgg cccgcaataa aaagattcct ttagtatatg
    10321 ttaaacccta tgctttgagt gaaccttttt ttcaatcaga gcatgcgctg atgccccacg
    10381 agttacgttt cttcggccct ttaagcaatc ccctttggca ctggacaaaa aagcgtatag
    10441 taaatacccg atggatcccc aatgccttca aacacgctgc acaaaaagaa atgcttcctt
    10501 taccaaaaac aagtttacaa atgatggatg ctgatctaaa tctcatcaca acgcctaaac
    10561 tatttttgga agaagaacag ctgccggaaa attatcgaat cgttggtcca atttttgcta
    10621 agctcagtac ttccttgcct gaagaagtgc taacttttat acagcgaaag caacaagaaa
    10681 gaaaacgtat tatctatttc gcaatgggta gttcaggcaa tccgcgtatg ttacgcaaaa
    10741 ttttgttctt cttacaaaaa caaccggatc tagcggttat tgcacctatt gactatctgc
    10801 ttgatgacat tcaatctgac tcagaagatc ttttaataac agaatatctg tctagtcatc
    10861 ttttgcatga attcattgat ttttctttta tccacggcgg ggaagggacc gtccaaactg
    10921 cctgtgaatc cggcaaaccg tttatcggtg tggggatgca atttgagcaa agctgtaata
    10981 ttgactattg tgtaaaatac ggaaacgcaa tcgccctcac aaaaccagtg actgaaagaa
    11041 aactgcagat tgctctgcgc gaagtttctt cacccaagat tcgtcagcag gcacgcacgc
    11101 tgcaagccgt ttttccaata aatggtccac aacaagcaat tcaagaaatc gaaaccttcc
    11161 tgagcaacca cccaatatag tttcaagtag aaaaagcttt tttaatagag actcatcagt
    11221 aatttcgcta ttcgagaccc agacagtgaa aatacaaaaa aagtaaatag tggattgaaa
    11281 agttagagtt acttaaatta cggtgtctaa aaaacacaaa atcatcctta aaataaaaga
    11341 tctgacgcaa tccaaatgag cataggtagc gtctgccatt tattcttagt caaattcttt
    11401 gacacctttc tctggttgga tcatctcaac gtttcttcaa aagaaagacc ataataatta
    11461 ggcaaataca ttttcttaaa atgtgtcata tagatctaga agcatcgctt tgtagagatt
    11521 tgtgctgcta gtcccttcaa caattactaa atcaaatgta ggatttacca ctttatcttt
    11581 gataatcaac ctccctcttc tgttgagaca atgatctcgc aatccgatgc atactcctat
    11641 ataaactgcc agcaatcccc acatgctgag tagatcatgt cagaatgttc tatgtcgtca
    11701 gcaacgataa actaaaagtc acgttcacca tgactgattg ctattcccag cgcaattatt
    11761 tctatacacg tttcatgatt tgaataaaca ctctcatcat acatattacc attcatacac
    11821 catactgcag cccctactat atggttgtaa gctcccaaat ctatatttgc cttgatcact
    11881 ttggtagtag atcgactaat tcttaatcca ttgctgataa aattttcatt tttgcctcct
    11941 tctttctgcc aaaacagtta aaacaataat aatctttatg taaaaaaggg ctgtgacaaa
    12001 agccttgtca cagtcccata ttccgaataa acggtggtca aaagcttgca tctgcggtaa
    12061 taagttcaac caaacgaaaa ataagccaac atttcacaaa aatttaagaa gcaattttcg
    12121 taaatttctt cttatttctt gaagctaacc acttctgtca caaccacttt tagaacattt
    12181 aaagcttcga tgtgacaaac catctatgct gctcgatcat aaaattgata gcaaaaattc
    12241 cacaaagtac atatgtcccc catgaaaaca aaacattcat atgcggtaca atgatctttg
    12301 gaagaatcac gatcaaaaat gatgccagtg cccaaatcca tcgattgatt tgaatatgat
    12361 atgtagctag catattcaat ataaggtgac tagccgttaa taaaaagata aaaacaaaaa
    12421 ataaaatcag ccaatacatt gtttcccacc ttctaaaaac caaacttaga cagtttggac
    12481 ttcgttttct ccacgacttt gttcatctga cagcatcttt ttatcataag cttttacaaa
    12541 tggataatag ataataaatg ctatcaatgt gttgatcatg atcaagataa caacacgcca
    12601 gtcatttcct gctgaaagat aaccgccgat tggagctggt aatgtccaag ttggcaaaac
    12661 agtaaatcta ttgaccattt ctaatttgac tgcaaaataa ctgataatag tcaaaactaa
    12721 aggaactaag ttgaacggga tgaacatgta aggattcaac atcataggag ctccaaaaat
    12781 catgggctca ttgatattga agataccagg aataatagaa aaacgaccaa cctctttcaa
    12841 gaattttgat ttacagaaga atataaacaa aacagatacc acgatcgttg ctcctgcacc
    12901 accaattgtt accgtccatt gataaaactg ttcaggagcg atatagggta attgggtggc
    12961 agctgtacca tctgccaatg cctttgcatt ctcatccaac atgatcagcc acattggacg
    13021 aacaacagcc cctacaatac tcatgccatg gataccaaat gaccacaaca aatggatcaa
    13081 aaacattggt agtaaaacac caagcaaatt attccctaaa atattggtta aaggctcgaa
    13141 taacgataat aaggtagcgt ttacatcaaa gcccgcgacg cttcgaatca gccaagtaat
    13201 tgttaaaatg actgcacctg gaatcaatgc ctcaaaacta cgggcaacag caggtggcac
    13261 ttgttctggc attttgatag taatatttcg atttttaaag aaacgatata cttccactgc
    13321 aaaaatcata gataagatcc ccgcaaacat cccagatgct cccaaagaac tcataggtaa
    13381 gacccagcca agaggtgcct tttcaggagt taaaacgttt acaggaactt gtaacataaa
    13441 gaacgttcct aaagataata taccacctgt cacactatct aatccatatg atttagctag
    13501 atccgaaccc ataacaaaag ttgcataaat agccatcaat cccatcgtca tcctaaaagg
    13561 taaaacaatc gttgccgaat aaggggcaat aaattcattc catccaggaa acggtaaatt
    13621 gataaagacc ataaaaaatg tcccaatcaa aatcaatgac aatgttgaaa caaccccatt
    13681 acgaattgct aataaatgac gctgtgtacc aatttttgcc attggtggga caagtttctc
    13741 ttcgacccat ttcatcagct tttccactaa aaccactcct tttcaataaa atatgaacca
    13801 ttcattgcat gacaacgc
//
LOCUS       DAZKKW010000068        13732 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.069, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000068 DAZKKW010000000
VERSION     DAZKKW010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13732
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.069"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(143..1345)
                     /locus_tag="ACG8PO_002000"
     CDS             complement(143..1345)
                     /locus_tag="ACG8PO_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0207302.1"
                     /translation="MKTKYPCVYQDKNGRFSYLVELGTDQVTGKRIQKKGTKDSLGKR
                     FSSAREAHKEVMRIKNEYMVVNGFANYDLTYKEFMEQSYVPHYKASVQKSTWSSRECG
                     LIQITEFFGDKKLREINVRDCENYRIWLLNDSGYSQAYSSLMYGMFRKSLDYAVTLQF
                     LNENISKRTKAIPKGKSIVPYWTKEEFEEVLAKIYKDDFYEHMCFLMIWLYYMTGVRV
                     SEGLALNWNDVDFKRKKLRVHHTLEMTNQKDFIRKPYTKTESGLRIISLDDDTIEVLK
                     KWKVIQKNHGVEQFILSYTDLPLYRSTVQRIIQRYAKLAKVPLIQGKGLRHSHVSYLI
                     NEFNADILVVSQRLGHSSPEITLKHYAHLWSRNDEGIAEQMTGNIKFTCATKSKVANF
                     NGNQAVKI"
     gene            complement(1348..1542)
                     /locus_tag="ACG8PO_002001"
     CDS             complement(1348..1542)
                     /locus_tag="ACG8PO_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFQ0207303.1"
                     /translation="MKNTICRKDLEALGFKTHQATTIIRQAKTRMVNQGYPYYNNKRV
                     GVVPRQVIEEIIGVPLSQEE"
     gene            complement(1589..2437)
                     /locus_tag="ACG8PO_002002"
     CDS             complement(1589..2437)
                     /locus_tag="ACG8PO_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rep protein"
                     /protein_id="HFQ0207304.1"
                     /translation="MQAYNQKIIETPTYIEIYDYEAIITPKRKTIENEVNLERKQKLA
                     WIYENDITLEKRKNYDELSAQGQYDSLKRKQKHYQSMRFEIARLVDTNFDNQTKFLTL
                     TFKENIQDIEYANNEFKKFIKRLNYDLYKTKKANLKYLATWEKQQRGAIHYHIILFDF
                     PFISFNRLTTIWGQGFVKINKIDVDSIENRGRYISKYFDKELELKEHKKKAFFKSRNL
                     KLPTVTKKMVKKPYDSTNQDILYTSEYIVRRPFFYNALALNELGSPEQVMDFQESTVH
                     YTKIKK"
     gene            complement(2642..2992)
                     /locus_tag="ACG8PO_002003"
     CDS             complement(2642..2992)
                     /locus_tag="ACG8PO_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0207305.1"
                     /translation="MPIKITDLSLPKETLEKEYQVVNVTRWQKEGEILGWSYECILPK
                     LRYEKISVKVKSEHPVITPEELTQQGLAIITFTNLVIVPWGRSNGQFVSYGLSATADT
                     ATLIPRKQGTTTQN"
     gene            3170..4033
                     /locus_tag="ACG8PO_002004"
     CDS             3170..4033
                     /locus_tag="ACG8PO_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0207306.1"
                     /translation="MFGLVQPNKVKVGQRIKEIKDGMSLSFTELGNRLGLKKPTISSY
                     VQGYALAPKSVINQLSSISGKPVGWFYFGHVEEYIREYLFLIGQKKIVDDYPEIIEKI
                     KQEFYTGEFKNPAWENELGYPLEEFIQECFVEYSSEIMKDYVVQITKEVLEESKTLNK
                     LSSKAKEEAVTILSSGIIDYIEMSGEIKYGEKDRIAKMVKESIKTFDVKQDLTFNDSY
                     LIGKLINILGTDEDTELFLSSLSMIFTDKHFSTRFGGEELVEIFQSMRPALIKLYREK
                     TPDEFYDWFEK"
     gene            4657..5649
                     /locus_tag="ACG8PO_002005"
     CDS             4657..5649
                     /locus_tag="ACG8PO_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0207307.1"
                     /translation="MYWEEIELEKTLGPGGEIRAVQRDKKNISKWEKLKKQLRRDKWM
                     YLLLLPGIIMTFIFKYVPMYGAVIAFKDYNPMLGILESPWVGLDHFREFISSPNFTLL
                     LSNTLKISIYGLLLGFFPPIILALSLNQIINKKIKQKIQLILYAPNFISVVIIVGMLF
                     LFLSANGPINGLVEKVTGQPIMFMSNPAYFRTIYILSGIWQGIGWSSILYTATLAGVS
                     TDLYEAANIDGANILQKIWHIDLPALRPVMVISFILAAGGIMNVGYEKAYLMQTSMNI
                     PASEIISTYVYKVGLQSGDYAYSAAVGLFNMIINVVLLLIVNNITKRLNDGQGL"
     gene            5665..6549
                     /locus_tag="ACG8PO_002006"
     CDS             5665..6549
                     /locus_tag="ACG8PO_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0207308.1"
                     /translation="MNSALSKIDRQILWLNRVIVTFLVVITIVPLIYVLVASFMDPAT
                     LINKGISFNPKDWTIQGYQRVFADDSILRGFVNSLFYSFSFSLLTVFITMITAYPLSR
                     PNLAGKKIIMTFFVITMFVGGGLIPTYLLVKNLGMLDSVWAIIVPGAINVWNIILART
                     YFSALPKELSEAATIDGANDLQILFKVMLPLAKPIMFVLFLYAFVGQWNSYFDAMIYI
                     KDPDLQPLQLVLRNILIQNQPSQDMVGANTAMAEMKQIAELIKYATIVISSLPLIIMY
                     PFFQKYFDKGVLVGSVKG"
     gene            6598..8205
                     /locus_tag="ACG8PO_002007"
     CDS             6598..8205
                     /locus_tag="ACG8PO_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0207309.1"
                     /translation="MKAKWMLPIVFTSVLILGACGGSKEKSKSSPDYELENVSFPLKE
                     KVSLKMMSQSAPLAPNDPNDKLIFQRLEEETNLHIDWTNYSSDFAEKRNLDIASGDLP
                     DAIFNAAAGDYDLLNWAESGVIVPIEDLIEDYMPNLKKIFDENPEYRQMSTAPDGHIY
                     SMPWIEELGEGKESIHTVNGMAWINKEWLDNLGLEMPQTTDQLMTVLEAFKTQDPNGN
                     GEADEIPFSFINNGGNEDLKFLMGAFGLGDNDDHLVVDNDGQVQFTANKEGYKEAIKY
                     FNEMNNKGLIDKEAFEQDWNTYVAKGKEHKYGVYFTWDKANITGADDSYDVLPVLAGP
                     DGTKHVTRTNGMGFSRDRFVITSANKNLELTARWIDKMYEPLQSVQNNWGTYGDEAQA
                     NIFELKDGMLKHLPLEGTAPGELRQKTEAGGPLAVLDSYYGTVTTMPDDAKWRLDLMH
                     DTYLPYITEDNIYPRVFLEKEETDRLAKIETDMWDYIYRKRAEWITGGKIDEEWEEYL
                     AELERVGLEEWIQIKQTGYDNFLKGAQ"
     gene            8206..9690
                     /locus_tag="ACG8PO_002008"
     CDS             8206..9690
                     /locus_tag="ACG8PO_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016621147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HFQ0207310.1"
                     /translation="MLVEETAILDKANRFIEMMRGKVNPQYRQNYHFSAPIGWINDPN
                     GFIYYKGHYHLFYQYYPYDSRWGPMHWGHARSSDLVHWEDLPVALAPDQPYDSGGCFS
                     GSAIERDGKLYLFYTGCQEIDGISHQHQCLAVSEDGIHFSKYSGNPVVAEKQLGENGL
                     IQEFRDPKVIVHNDGYYMVVATKSTQSTGRIVMFQSSDLYTWHFYSVLLEGTSEQGIM
                     WECPDLFHLDGRDVLIMSPIQIKKNGLEYHNISSTMACIGEVNWETGQMIVDNFHEMD
                     FGMDFYAPQTTRDPNGGRVMIAWMQMWDRTLPTHELGHLWAGSMSLPRELRVKKNRLV
                     QKPISTVYSHLVYQQGSENIAVTDTSVVFRNALSDSSYLHLVADLEKAEVFTIELLRS
                     KTDCLKLAYDTATQVFELDRQKLGRELTGAEEDPLNVRKVEVPLIDNQLVLEIFRDTS
                     SIEVFINGLEVMTATFYELEKARDILFHSIGESRIQAFETASVK"
     gene            9867..10859
                     /locus_tag="ACG8PO_002009"
     CDS             9867..10859
                     /locus_tag="ACG8PO_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005230454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0207311.1"
                     /translation="MKATMKDVAKLAGVGVGTVSRVLNNGSVKASTREKVEAAMTELN
                     YQPDFYARGLKMKHTNTVALILPTVWHPFFGEFAYHVETALERQNYKLYLCNSSGNSE
                     KEQEYIQMVSQNKVDGIIGITYSDIDEYISSNLPFVSIDRYFTEEVIYVTADNAAGGR
                     LAANELYRRGCQNVAYIGGYQETPNETKSRRKYFEIECRQLGISYKALDMLEPLDHLS
                     ERVTNFLLENPDIDGIFTINDPMALAVIDMLEKIGKNVPEDVQVIGFDGQKMTEGDPY
                     LVSTIAQPTKQMAEVAVAKLIAVINGETVEKRTVLPIEFGEGDTTKPLVRNDFA"
     gene            10911..11780
                     /locus_tag="ACG8PO_002010"
     CDS             10911..11780
                     /locus_tag="ACG8PO_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005472675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFQ0207312.1"
                     /translation="MLYGGIEAGGTKFVCGIGNEQLQVIERKSFPTTTPEETMEQVFD
                     FFDKYKGNIASIGVGAFGPIDIQIESRTYGYITNTPKLAWQNYHFVGALENALSVPIY
                     WTTDVNAACYGEYMAGYGKGKNSVLYYTVGTGVGGGAVVDGKIVAGFSHPEMGHMLLQ
                     RHNEDQFEGICPFHHDCLEGLASGPAIEKRFGRKAQTISFDHPYWTIEADYLAQCVYN
                     TTLLYAPEVIILGGGVMKQPQLLELIRQSFKEKMGSYVDTPTLEEYLLTPRLADNAGL
                     IGCLALAIKKLNH"
     gene            complement(12200..>13732)
                     /gene="guaA"
                     /locus_tag="ACG8PO_002011"
     CDS             complement(12200..>13732)
                     /gene="guaA"
                     /locus_tag="ACG8PO_002011"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFQ0207313.1"
                     /translation="KIIVLDYGSQYNQLITRRIREFGVFSELMSHRITAEEVKAMNPK
                     GIILSGGPNSVYDEKAFGIDEKIFDLGIPVLGICYGMQLITYKLGGKVKPAKNREYGK
                     AELEITSAEAALFQNTPEKQTVWMSHGDLVTQAPTDFDVVATSKDCPIASIQNAERKM
                     YGIQFHAEVRHSEYGNDLLRHFTLDVCQCTGDWTMDNFIDMEIAKIREQVGDKKVLLG
                     LSGGVDSSVVGVLLQKAIGDQLTCIFVDHVLLRKGEAEQVMESLGGKFGLNIIKVDAK
                     ERFLSKLAGVSDPEEKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQGTLYTDVIESGT
                     ETAQTIKSHHNVGGLPEDMQFKLIEPLNTLFKDEVRELGTQLGMPDSIVWRQPFPGPG
                     LGIRVLGELTEEKLEIVRESDAILREEIANAGLDRDIWQYFTVLPGIRSVGVMGDGRT
                     YDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVYDITSKPPATVE
                     WE"
BASE COUNT         4214 a         2322 c         2832 g         4364 t
ORIGIN      
        1 ttgaaaaacg ttgatttatc ggcattcccc atccatatta tatttacgtc ttattcccac
       61 tcaatatgag tagcctcgta aagaagctat tatatcagtg ctttttcagc ctattcctca
      121 ttttggtagg gaaatggtag ggttagattt ttactgcttg atttccatta aagtttgcaa
      181 ctttagattt tgttgcacag gtaaatttaa tgtttcctgt catttgttct gcaattcctt
      241 catcattacg gctccataga tgagcgtagt gctttaaagt gatttcaggg ctagaatggc
      301 ctaaccgttg cgacacgacc agaatgtctg cgttaaactc attgattagg tagctaacat
      361 gagaatgacg taatcctttt ccttgtatta ggggtacctt agctagtttg gcataacgct
      421 gaataattcg ttgaacagtt gaacgataga gtgggaggtc agtgtagcta agaataaatt
      481 gttctacacc atgatttttt tgaataactt tccacttttt tagcacttca attgtatcat
      541 cgtccagaga aatgatccgc aagccgcttt cagtttttgt gtaaggtttc cgaataaaat
      601 ctttttgatt tgtcatttcc aaggtgtgat gaaccctcaa ctttttacgt ttgaagtcca
      661 cgtcattcca attaagtgct aatccttcgc ttacacgaac accagtcatg taatatagcc
      721 agatcattaa aaaacacata tgttcgtaga aatcatcttt ataaattttt gccaaaactt
      781 cttcaaattc ttcttttgtc cagtaaggaa cgattgattt tccttttgga attgcttttg
      841 ttcttttgga aatattctca ttcagaaatt gcagggtgac cgcatagtct aatgatttac
      901 gaaacatacc gtacataagt gaactgtatg cttgtgagta tccgctatcg ttcaataacc
      961 aaattcgata attttcacaa tctcgcacat tgatttcacg aagcttctta tcaccgaaaa
     1021 attcagtaat ctgaatcaat ccacactcac gagaactcca agtacttttt tgtacgctag
     1081 ctttgtagtg tggaacgtaa gactgctcca taaattcttt gtaagttaaa tcataattcg
     1141 caaatccatt tacaaccatg tattcatttt ttatacgcat gacttctttg tgtgcttcac
     1201 gagctgatga gaatcgtttt cctagagaat cttttgttcc tttcttctgg atcctctttc
     1261 cagtcacttg atctgttcct aactcaacta gataagaaaa gcgaccattt ttatcttgat
     1321 acacacaagg gtattttgtt ttcatagtta ttcctcctgt gaaagaggaa cacctataat
     1381 ttcttcaatt acttgtcggg gtactacccc aactctttta ttattataat atggatatcc
     1441 ctgatttacc attcttgttt ttgcttgtcg gattattgtg gtagcttgat gtgttttaaa
     1501 tcctagagcc tctaaatctt ttcgacaaat tgtatttttc attgtataaa ctcactttca
     1561 aagaaaacac tatcattggg taatagtgtt atttctttat ttttgtataa tgaacggtac
     1621 tttcttgaaa atccatcact tgttcaggac ttcccaactc atttaaagct aaagcattgt
     1681 aaaaaaatgg acgtctaact atatattcgc ttgtatatag tatatcttga ttcgtggaat
     1741 catagggttt ctttaccatc tttttggtga cagttggtag tttgagattc cgggacttga
     1801 aaaaagcttt tttcttatgc tctttcaatt ccagctcttt atcgaaatat ttgctgatat
     1861 agcgcccgcg attttcgata ctgtctacat cgatcttgtt gatcttaacg aaaccctgac
     1921 cccaaatcgt agttaaccgg ttaaatgaga taaaggggaa atcaaacaag ataatatggt
     1981 agtggattgc accgcgttgc tgcttttccc acgtggcgag atactttaaa tttgcttttt
     2041 tagttttata gaggtcataa ttcagtcgtt tgataaattt tttaaattca ttatttgcat
     2101 actcaatgtc ttgaatgttt tctttgaaag tgagagttaa aaatttcgtt tgattgtcaa
     2161 aattcgtgtc tacaaggcgg gcaatctcaa atcgcatact ttgataatgt ttctgtttgc
     2221 gttttaaact gtcatattgt ccttgagcgg ataattcatc atagtttttt cttttttcaa
     2281 gagtaatatc attttcataa atccaagcta atttttgttt tcgttccaga tttacttcgt
     2341 tttcgatcgt tttacgtttg ggagtaatga ttgcttcata atcataaatt tcaatatacg
     2401 tgggagtttc aatgattttc tgattataag cttgcaaaaa tcatcactcc ttgttgcgta
     2461 ttcgattgtc aaagaaccag tcattttctc ctgatgttga tctataaatc aagttaaggg
     2521 ggaaggcctg cgtagcagcc cttctagcct atggggtgtt gggcggtctt aacggacaga
     2581 aacggctctg tgggcgctgc cccgccgttt cgtcctaacc acccaacact ccatagtata
     2641 gttagttttg tgtagtggta ccttgttttc tgggaatcaa agttgccgta tcagcggttg
     2701 ccgataaccc ataactgaca aattgtccat tcgagcgtcc ccaaggtaca atcaccaaat
     2761 tggtaaatgt aattatggct aaaccttgtt gggtaagttc ttccggcgtg atcacaggat
     2821 gttcagattt gactttgaca ctgatttttt cgtagcgaag ttttggtagg atacattcgt
     2881 agctccaacc tagaatttcg ccctcctttt gccagcgagt gacattgacg acttggtatt
     2941 ctttttcgag tgtttctttt ggtaaactga gatcggtaat ttttattggc atcacaattc
     3001 tcctttttta tacgaaagtt tatacgattt gattaaaaaa tatagatggc catctacgac
     3061 ttgtattata tacgagaact tatacgtagt caagagattt tctaaaaaaa tatatacgat
     3121 tttttatacg agtgttataa tgaaaaaaat gataaggagg aatttgaata tgtttggttt
     3181 agtacaacct aataaagtga aggtgggcca acgaatcaaa gaaataaaag acggaatgtc
     3241 tctctctttt acagaattag ggaatcgatt aggtttaaaa aagccgacga tcagttccta
     3301 cgttcaaggc tatgcgttgg ctcctaagag tgtgatcaac caattatcta gtatcagcgg
     3361 aaaacctgtc ggctggtttt attttgggca tgtggaagaa tatatccgag agtatttgtt
     3421 tctcattggt cagaaaaaaa tagtggacga ttatcctgaa atcattgaga aaataaagca
     3481 agaattttat acgggcgagt tcaaaaatcc tgcttgggag aatgagctag gctatccttt
     3541 ggaggaattt atccaagaat gttttgtaga atattcgagt gaaatcatga aggactatgt
     3601 cgtacagatt acgaaagagg tacttgaaga atccaaaacg ctaaacaaac tatcatcaaa
     3661 agcaaaagaa gaagctgtca cgattctttc atctgggatc attgactata tcgagatgag
     3721 tggtgagatc aagtatggag agaaagatcg cattgcgaaa atggtgaaag aatcaataaa
     3781 aacctttgat gtgaaacaag acctgacatt taatgattcc tatttgattg gaaagttgat
     3841 caatattctt ggtacagatg aagatacgga gctgtttctt tcttctttat caatgatatt
     3901 tactgataag catttttcca ctcgatttgg tggggaagaa ttagtcgaaa tatttcaatc
     3961 gatgcgacca gcgttaatca aactctatcg ggagaaaaca ccagatgaat tttatgattg
     4021 gtttgagaag taaaataaga aaagtggctt agattacaag ttggtgaaaa aatttttttc
     4081 agcataacat cagtaatcta taaaggggtt gttgattata ctgtagaagt taagtactca
     4141 agagattttt tgtacgtaaa caaagcaaaa aagggtgcag tttcactaga aaatgtactc
     4201 ttttttgtga tttttcttag ttatagagtt tttttgaaac tttaaaaatg agaaaacata
     4261 ttgattttaa agagtgtaga catagcttct tttgctaaat agattaaact tactggtgag
     4321 tggtcttgaa catactttta aatcacattt tactgagttt tattggattt tataattcat
     4381 tggaaacctc gtacttaatc tgtttttatc tatcgactag aatctgtata tattccagct
     4441 atcaatgagg ccgcagaaaa gggtgcttaa aaataatctg ttgtttaaaa tattattatc
     4501 agaatactta gatacaaaat tctggcacaa agtaaaaaaa atattgactt aaaaaaatgg
     4561 atatggtaat ctataaacga aaatagtggt aacgtttcca aaatgcatct tcgttaaatt
     4621 tttatttttt taaacgaaaa tggaaacgtt tctaaaatgt attgggagga gattgaattg
     4681 gaaaagacat tgggaccagg tggtgagata agggcggtac aacgggataa aaaaaatatt
     4741 tcgaagtggg aaaaattaaa gaaacagttg cggcgtgata agtggatgta cttgttgctc
     4801 ttaccaggta tcatcatgac gtttatcttt aagtatgtgc caatgtacgg tgctgtgatt
     4861 gcatttaaag actataatcc tatgctagga attttggaaa gtccttgggt aggactagat
     4921 cactttaggg aatttatcag ttcaccaaat tttacgcttt tgctgagcaa cacattaaaa
     4981 attagtattt atggtttatt gttaggtttt tttccaccaa ttattttagc actgagcttg
     5041 aatcaaatta tcaataaaaa aataaaacag aaaatccagt tgattctata tgcgccgaat
     5101 tttatctcag tagttattat tgtggggatg ctgtttttgt tcctatccgc aaatggacct
     5161 attaatgggt tagtagaaaa ggttacgggg caaccaatta tgttcatgtc taatccagct
     5221 tatttcagaa cgatttatat tttatcggga atttggcaag gaatcggctg gtcatccatt
     5281 ctttacaccg caacgctggc gggagtcagt accgatttat atgaggcagc aaatatcgat
     5341 ggggcaaata ttttacaaaa gatttggcat atcgatttgc ctgcattgag gcctgtgatg
     5401 gtgatcagct tcatcttggc cgccggcggt attatgaatg taggatatga aaaagcctac
     5461 ttgatgcaaa cttcaatgaa catccctgcc tcagaaatca tttccactta tgtatacaaa
     5521 gtcggcttgc aatcaggtga ttatgcctac tctgctgctg ttggtctgtt caatatgatt
     5581 attaatgtcg ttttattact gattgtcaac aatataacca agcggctaaa cgatggtcaa
     5641 ggcttgtaat gaaaggagaa aattatgaat agtgctttat caaaaatcga ccgtcaaatc
     5701 ttatggttga atcgagtgat tgtaactttt ttagtcgtca tcaccattgt tccgctgatt
     5761 tatgtactgg tagcttcttt tatggaccca gctactttga tcaataaagg tataagcttc
     5821 aatccgaaag attggacaat acagggatat caacgggtat ttgcagatga ttccatttta
     5881 cgcggttttg taaattcgtt gttttactct tttagcttca gcttattgac tgtatttatt
     5941 acgatgatca ctgcgtatcc tttgtctcga cctaacttgg cggggaaaaa gattattatg
     6001 actttttttg tcattacaat gttcgtgggt ggtggcttga tacctaccta tttattggta
     6061 aagaacttgg gcatgttaga tagtgtatgg gcgattatcg taccaggtgc tataaatgtg
     6121 tggaatatta tcttagcccg tacctatttt agtgctttgc caaaagaact ttctgaagct
     6181 gctactatcg atggcgccaa tgacttgcaa atactgttca aagtcatgct tccattagcg
     6241 aaaccaatta tgttcgtatt gtttttatat gcctttgttg ggcaatggaa ctcatacttt
     6301 gatgccatga tttacatcaa agatcccgac ttacagccgt tgcaactcgt attaagaaat
     6361 attttgatcc aaaaccaacc ttcacaagac atggtgggag ccaatactgc catggcagaa
     6421 atgaaacaaa ttgctgagtt gatcaaatac gcaacgatcg tcatttctag tctgccatta
     6481 atcataatgt atcctttctt ccaaaaatat tttgataaag gtgttttagt tggttctgtt
     6541 aaagggtaag tattgttcac tatttgtatg aataaaaatt ataaaggaga ttttaagatg
     6601 aaagcaaaat ggatgctgcc aatcgttttt acttccgtac taattttggg ggcttgtgga
     6661 ggctcaaaag aaaagagtaa atcaagccct gattatgaac tagaaaatgt ctcttttcca
     6721 ttgaaagaaa aagtatcttt aaaaatgatg tctcaaagtg cccctttggc tcccaatgat
     6781 cctaatgaca aattgatttt ccaacgatta gaagaagaga ccaatctcca tattgattgg
     6841 acaaactaca gtagtgattt tgccgaaaaa agaaatttgg atatcgctag tggtgattta
     6901 ccagatgcca tcttcaacgc cgctgcaggt gactatgatt tattaaactg ggcggaaagt
     6961 ggcgttattg taccgattga agatttgatc gaagattata tgccaaatct gaagaaaatt
     7021 tttgatgaaa accccgaata ccgacaaatg tctactgcac ctgatggtca catttactct
     7081 atgccttgga ttgaagaatt aggcgaagga aaagagtcga ttcatacagt aaatgggatg
     7141 gcttggatca acaaggagtg gttggataat ttgggtcttg aaatgccgca aaccactgat
     7201 cagttgatga ctgtgttgga ggctttcaag acgcaagatc caaatggtaa tggtgaagca
     7261 gatgaaattc cttttagctt tattaacaat ggaggaaacg aagacttaaa atttttaatg
     7321 ggggcattcg gtctaggtga taatgatgac cacttagtcg ttgataacga tggtcaggta
     7381 caatttactg ctaacaaaga agggtataaa gaagccatca aatatttcaa tgaaatgaat
     7441 aataaaggtc ttattgataa agaggctttc gaacaagatt ggaatacgta tgttgccaaa
     7501 gggaaagaac ataagtatgg cgtgtacttt acttgggata aagcaaatat cacgggtgca
     7561 gatgatagtt atgacgtctt gccagtctta gcgggaccag atggaacgaa acatgttact
     7621 cgtacgaatg gcatgggttt ctcaagagat cgtttcgtaa ttactagtgc gaataaaaac
     7681 ttggaactta cagctaggtg gatagataaa atgtatgaac cgttacagtc tgtccaaaat
     7741 aactggggaa cctacggtga tgaagcacag gcgaatattt ttgaattaaa ggatggtatg
     7801 ttaaaacatt tgcctttaga ggggacagca ccgggggaac taagacaaaa aaccgaagct
     7861 ggaggaccat tagcagttct tgattcttat tacggcacag tcactactat gccagatgat
     7921 gcgaagtggc gtttagattt gatgcatgac acgtatttgc catatattac agaagacaat
     7981 atctatccac gtgtcttttt agaaaaagaa gaaacagatc gattagcaaa aatcgaaacg
     8041 gatatgtggg attacatcta tcgcaaaaga gcagagtgga tcaccggcgg taaaatcgat
     8101 gaagaatggg aagaatacct tgctgaattg gaacgtgtag gtttggaaga atggattcaa
     8161 ataaaacaaa ctggctatga taattttttg aagggagcac aataagtgtt agtagaagaa
     8221 acggcgattt tggataaagc aaatcgattt attgaaatga tgcgaggaaa agttaaccct
     8281 cagtatcgac aaaactatca tttttccgca cccatcgggt ggataaatga ccccaatggg
     8341 tttatatatt acaaagggca ttaccatctc ttctatcaat actatcctta cgatagtcga
     8401 tgggggccca tgcattgggg gcatgcgcgc agtagtgatt tggtccattg ggaagatcta
     8461 ccagtagcgt tagccccaga tcaaccttat gacagcggcg ggtgtttctc aggaagtgca
     8521 attgaaagag acggaaaact atatttattt tatacaggct gtcaagaaat tgatggtatt
     8581 agccatcagc atcaatgttt ggcggtctct gaagatggta ttcattttag caaatacagt
     8641 ggtaatcctg tggtagcaga aaaacagcta ggtgaaaatg ggctaataca agaatttcgt
     8701 gatcctaaag taattgttca caacgatggt tactatatgg tagtagctac taaatctaca
     8761 cagtctactg gtagaatagt gatgtttcaa tcgtcagatt tgtatacttg gcacttttac
     8821 tctgtgctgc tagaagggac ttcagagcaa ggaataatgt gggaatgtcc tgatttattc
     8881 catttagacg gacgagatgt cttaatcatg tctccgatac aaatcaagaa gaacggactg
     8941 gaatatcata atatcagttc taccatggct tgtattggtg aggtaaactg ggaaacaggt
     9001 cagatgatag ttgataattt ccatgaaatg gattttggaa tggattttta tgcaccacaa
     9061 acaacaagag atcctaatgg tggacgggtg atgattgctt ggatgcagat gtgggatcgt
     9121 acattgccta cacatgagtt aggccatctt tgggcaggct ctatgagttt gccccgagag
     9181 ttaagagtga aaaaaaatcg attggtacaa aaacccatct caactgtata tagtcattta
     9241 gtttatcaac aaggcagtga aaatattgca gtgactgaca catcagtcgt atttcgaaac
     9301 gcgctgtctg attcttcata tttacatcta gtcgcagatt tggagaaagc tgaagttttt
     9361 acaattgaat tactgcggtc taagacggat tgtctcaaac tagcctatga cactgctaca
     9421 caggtttttg aattggatcg tcagaaacta ggaagagagc ttacaggggc ggaagaagac
     9481 cctctgaatg tacgaaaagt tgaagtacct ttgattgata atcagctcgt tcttgagatt
     9541 tttagagata cctcatcaat tgaagtcttt attaatgggt tagaagttat gacggctact
     9601 ttctatgagc tggaaaaagc tcgagatatc ttgtttcatt cgatcggaga aagtcgtatt
     9661 caagcttttg aaacagcgag tgtgaaataa atgtttgatt gcttaggcga tgaatcaatt
     9721 atttaatagg ttttgaagga taatgttttt agaaaagaac agatagctag atttcccatg
     9781 gatttgctat tagtattttg aaaaaattag taatttatga caaaattcat tatactaaac
     9841 ttgtgcagaa atgtgaggga tcaactatga aagcaacaat gaaagatgtc gcaaaattag
     9901 caggtgttgg agtaggaact gtttcccggg tattgaataa cggttctgtt aaagcttcta
     9961 ctagagaaaa agttgaagca gcaatgacag agttaaacta tcaaccagat ttttatgccc
    10021 gggggttaaa gatgaagcat acgaacacgg ttgctttgat tctacccacg gtatggcatc
    10081 cattttttgg cgaatttgcc taccatgtag aaacagcatt agaaagacaa aactacaagc
    10141 tttatttgtg taactccagt ggtaatagtg aaaaagaaca agaatatatc cagatggtca
    10201 gccaaaataa agtagacggc atcattggaa ttacttattc agatatcgat gaatacattt
    10261 cgagtaatct gccttttgtt agtattgacc gttatttcac tgaagaggtg atttatgtga
    10321 ccgctgacaa tgcggccggt ggacgtttgg ctgcaaatga actttatcgc cgaggatgtc
    10381 agaatgtagc atatatcggt gggtaccaag aaacaccgaa tgaaacgaag agtcgtcgaa
    10441 aatattttga aatcgagtgc cgtcaactgg ggatttccta taaagcgtta gatatgttag
    10501 aacctttaga ccatctctca gaacgtgtaa caaatttttt gttagagaat cccgatatcg
    10561 atgggatttt tacgatcaac gatccaatgg cattagctgt cattgatatg cttgaaaaaa
    10621 tagggaaaaa cgtaccagag gatgtccaag ttatcggatt tgatggtcaa aagatgacag
    10681 aaggagatcc ttatctagtc tcaacgattg cccaacctac aaagcaaatg gcggaagtgg
    10741 cagtggcaaa attaattgct gtcattaatg gagagacagt agaaaaacgt accgtattgc
    10801 cgattgaatt tggcgaaggt gacacaacga aacctttagt gagaaacgat tttgcttagt
    10861 gcaaatacgt ttcttttttt gaaaatgagt gaatgaaagg tgtagataaa atgttatatg
    10921 gtggaataga agccggtgga acaaagtttg tctgtgggat tggtaatgaa caattacaag
    10981 tgatagaacg gaaaagtttt cctaccacaa caccagaaga aacgatggag caagtgtttg
    11041 atttctttga taagtacaaa ggaaatatcg catcaattgg tgtaggagca tttgggccta
    11101 ttgatattca aatcgaatca agaacttacg gatatatcac caacacaccg aagttagctt
    11161 ggcaaaatta tcattttgta ggagctttgg aaaacgcctt gtcggttccc atctattgga
    11221 ctaccgatgt aaatgctgct tgttatggag aatatatggc agggtatgga aaaggtaaaa
    11281 atagtgttct gtattacacc gttggtacag gtgttggtgg tggtgctgta gtggatggga
    11341 aaatagtcgc aggttttagt catcctgaaa tgggacatat gctcttacaa cgtcataatg
    11401 aagatcaatt tgaaggaatc tgtccttttc atcatgattg tttggaaggg ttagcatcag
    11461 gacctgcgat tgaaaaacgc tttggaagaa aagcccagac tatctcattt gatcatcctt
    11521 attggacgat tgaggcagat tatcttgctc agtgtgtgta taacacaacg ttactatatg
    11581 caccggaagt aataatttta ggcgggggtg taatgaaaca gccgcaacta ctcgagttga
    11641 ttcgccaatc gtttaaagaa aaaatgggtt cctacgtgga tacgcctact ctcgaagaat
    11701 atctactaac tccaaggctt gctgacaatg cgggcttgat tggctgccta gctttagcaa
    11761 taaagaaact caatcattag aatagtattt attagaacta ttcataacat accggaagtt
    11821 taaaagggta ttctgaaact taaatagtct caattcattc ttctttcttg agaatctgag
    11881 tagagacttt tattctgttt caaattaata taattacgaa atgatattga gtacaagaaa
    11941 gttcttttta ccagatcttt taaatcgaaa aatctgatat atatatttat agtactaaaa
    12001 atctgattag ataaaaagct aattcaatag gaaaataaag gtagtgaata aaagcaaagc
    12061 ccttgttagt agttaatcta acaagggctt tggtagggaa aatggtagga aaccatctta
    12121 ttttttacga aaatcaggaa ctttagtaat tgaataatgt tgatttatag gtattcctca
    12181 tccatattat gttggctctt tattcccact ccactgttgc aggtggtttg ctggtaatat
    12241 catacacgat acggttaacg tgtgcgactt catttacgat acggacactg attttttgca
    12301 agacttccca agggatacgg gcaaagtcag cggtcatgcc gtcaatagaa gtgacagcac
    12361 ggatacctac agtgtagtca tacgtacggc catctcccat aaccccaaca gagcgaatgc
    12421 ctggtaggac ggtgaagtat tgccagatgt cgcgatctaa accggcattg gcgatttctt
    12481 cgcgcaagat agcgtctgat tcacggacga tttctaattt ttcttctgtc aattcaccaa
    12541 ggacacgaat acctaagcct ggtccaggga atggttggcg ccaaacgatg ctgtcaggca
    12601 tgcctaattg ggtacctaat tcacgtactt cgtctttgaa caatgtgttc aatggctcga
    12661 tcagcttgaa ttgcatgtct tcaggtaatc cacctacatt gtgatgtgat ttgatggttt
    12721 gggcagtttc agttcctgat tcgatcacat cggtataaag ggttccttgt gcaaggaaag
    12781 aaacgccttc ttcaccggca agtttggttg cttcatcatc gaagacatat acaaattcat
    12841 ttccaatgat tttacgtttt tcttctggat cagacacgcc agcaagtttt gataagaaac
    12901 gttctttggc atcgactttt ataatgttca agccaaattt tccgccaaga ctttccataa
    12961 cttgttctgc ttcaccttta cgcaacaata cgtgatcaac gaagatacat gttaattgat
    13021 cgccgatcgc tttttgtaaa agaacaccaa caacagagga atctactccg cctgaaaggc
    13081 ctaataaaac ttttttgtca ccgacttgtt cacgaatttt tgcgatctcc atgtcgatga
    13141 agttatccat tgtccagtca ccagtacatt ggcagacatc tagtgtaaaa tggcgcaaca
    13201 agtcatttcc atattcggaa tggcgaactt ctgcatggaa ttggattccg tacattttgc
    13261 gttctgcatt ttggatcgat gcgatggggc aatctttgct ggtagcaacg acatcgaaat
    13321 cagtcggtgc ttgggtaact aggtcaccat gactcatcca aacggtttgt ttttcaggag
    13381 tgttttggaa aagggccgct tctgcagaag tgatttccaa ttctgctttt ccgtattcac
    13441 ggtttttggc tggtttaact tttccgccta atttataagt aatcaactgc ataccgtaac
    13501 agattcctaa aactgggatc cccaaatcaa aaatcttttc atcaatacca aaggccttct
    13561 catcataaac actgtttggt ccgccagaaa ggatgattcc tttagggttc attgccttta
    13621 cttcttctgc ggtaatgcgg tggctcatca actctgagaa aacaccgaac tcacgaatac
    13681 ggcgagtgat cagttggtta tactgactac cgtaatcaag tacgataatt tt
//
LOCUS       DAZKKW010000069        13305 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.070, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000069 DAZKKW010000000
VERSION     DAZKKW010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13305
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.070"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            64..2853
                     /locus_tag="ACG8PO_002012"
     CDS             64..2853
                     /locus_tag="ACG8PO_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HFQ0207314.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLVQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            2900..3427
                     /locus_tag="ACG8PO_002013"
     CDS             2900..3427
                     /locus_tag="ACG8PO_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HFQ0207315.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEETDHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            3448..4638
                     /locus_tag="ACG8PO_002014"
     CDS             3448..4638
                     /locus_tag="ACG8PO_002014"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFQ0207316.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNPVIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            4678..5976
                     /gene="asnS"
                     /locus_tag="ACG8PO_002015"
     CDS             4678..5976
                     /gene="asnS"
                     /locus_tag="ACG8PO_002015"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HFQ0207317.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            7152..7826
                     /locus_tag="ACG8PO_002016"
     CDS             7152..7826
                     /locus_tag="ACG8PO_002016"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFQ0207318.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     KFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTAEICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            7823..8164
                     /locus_tag="ACG8PO_002017"
     CDS             7823..8164
                     /locus_tag="ACG8PO_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0207319.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERVDQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSIFAQF"
     gene            8194..8553
                     /locus_tag="ACG8PO_002018"
     CDS             8194..8553
                     /locus_tag="ACG8PO_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HFQ0207320.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            8814..8936
                     /locus_tag="ACG8PO_002019"
     CDS             8814..8936
                     /locus_tag="ACG8PO_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0207321.1"
                     /translation="MNLDKLPATGFKLSCYPVKIKKASAGWIRAGAMIEEKKKE"
     gene            9078..11162
                     /locus_tag="ACG8PO_002020"
     CDS             9078..11162
                     /locus_tag="ACG8PO_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFQ0207322.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTTEGLMTVNPEDVQIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKKFMQYIASAEQFSTHPIAQSVLEGYAGPLLP
                     TTNIQEFAGEGILAEVDGKQVLAGNHKLMERFEISFPLSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQRNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLALGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKKEDE"
     gene            11453..12919
                     /locus_tag="ACG8PO_002021"
     CDS             11453..12919
                     /locus_tag="ACG8PO_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFQ0207323.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
BASE COUNT         4403 a         2310 c         2876 g         3716 t
ORIGIN      
        1 tttttctgca ctttgtagta taatttcaaa agaacattag aggcaggaga agaggaagat
       61 attttgaaac caagaactta tgcagtcgtt gatatcgaaa cgactgggac gaatccaaaa
      121 gaagatcgga tcatccaatt tggctgcgtc atgattgaat caggtcggat cacttcacgt
      181 ttttcgatcg atattcatcc agggcggaag atttcaaaac aaatccagca tttaacaggg
      241 atcacgaatc aacgcgtcca aaaagcgcct tatttcgaag atgtcgccca aacgatttac
      301 aatcttttgg cagacaccat atttgtcgcg cataatattt attttgatta taattttttg
      361 aaccatgaac tcatgcgatg cggacttcct agtttgaaga taccaggcat cgatacagta
      421 gaacttgcac aaattttctt gccgacggaa ccaagctttc gcttagctga tttgtcagaa
      481 agccttggat ttattcatga gaatcctcat caggcagata gtgatgccga agtgacaggt
      541 caacttcttt tgctgatcga agaacggatg agaaaattgc caatcatcac gttagagcag
      601 attgcgcgat taagtagaca cacaggtatg gatacgagtc gtttcattta tcatgtgatt
      661 gaagaaatga aagaaaaacc agagccactt gatccctcat tagaaatcgt cgatgggcta
      721 gctttgcaga aaaaagaagt ggaactattc acatccgtcc attatggcga gcggacatat
      781 ccaagaaaaa aacaagcaaa agaaaaaatg tttggtaaaa cactgatgta tcgaaaagaa
      841 cagaacagac tgatgaatgc ggtctacgac catttcacaa aagatgaatc aaaagatttg
      901 atgatcgaag cagctacagg aatgggtaaa acaatcggtt atctgctgcc attgagttat
      961 ttggctacac ctgaaaaacc cgctgtcatc agtaccgttt cgctagtttt gcagcagcag
     1021 atcatagaaa aagacattcc attgctgaat caattgctag atcagcccat acaacctgta
     1081 attatcaaaa gctatcgcca ctatattgat ttgcagcgtt ttaaaggcac acttgtggaa
     1141 ccgccagaac aaaaacaata tgctttgtat caaatggcgg tcttagtttg gctgacgcaa
     1201 acgaagactg gtgatttgga tgaattacat ttaacaaatt tgaaccattc attctttgca
     1261 gatatcgcgc acagaggtat tggattttta gcaagaaatc agtcatttta cgaacaagac
     1321 tttgttcgtt tcttgcaaaa gaaaatcagg caaagtaatt ttttgattgt caatcatgcg
     1381 tttttagtac aagaaacaca gcgaaaagaa ccattgcttc cagctagtcc atttttgttg
     1441 atcgatgaag tacatcattt gccggaaatc gctgaaaaag taaatgacaa acgattaagt
     1501 attaactatt tccataaaca aatccagcaa ttagaagaac caaatcagct gtttgaccga
     1561 atacaaagct atttaccgaa agaacatgaa gccttacgct ggttatcgat ttatcgagaa
     1621 gaactttctg cgctatgcga agcacagatt tgtatggcag aaggattaat gagtttagtg
     1681 agcggcaagc aacctaacgg acagtatcca gaagaagtca tgatgacaaa agagatgttg
     1741 gatcagctgc cactggaatc tgggacagcc attgatcacc tgcagctttt ttatcaggag
     1801 atccagcagc tacagtcaca aatgcgtctg acttttgaga ttatccaaga aagctggaca
     1861 aaccagatgc ggatcgattt agcaagttta tatagcctat ttgatcaatt agacaagcaa
     1921 tacttgctga tggatcaatg gctggaagac tggcgagatc atttgatcca ttggttcgtt
     1981 cctgggaaca cagcgcagca gatgattttt catctccatg attttcaagc ggctatgttg
     2041 ccgtcgactg tttggtatcc tcgatatgat cgtattcttt atattggagg aacgctgaag
     2101 atcggcccta atcgacacta tctctctaag aggcttggca ttgaagcagc acctcttaaa
     2161 gtgattgcct ctccttatga ttatgagaaa caggcaagga tctatgtgcc aaaagaggca
     2221 cctgccgtcc atcaagtcag caatcaagca tatatcgatt atttagagac agcacttaca
     2281 ggtctgatcg aacaagaaga tcggccaata cttgtgttat tcacttctca tgatgttttg
     2341 cagaaggtct atcagaaaat gcatctgtct atgctagaaa aaggacgaga aatactagct
     2401 caagggtttg gtggaagccg ggagaagctt ttgaaacgct tcattttgtc agaaaaaagc
     2461 gtattgtttg gcgctgacag cttttgggaa ggtgtagatt tgcctggaga tgctttgcag
     2521 atcgttgtag tcacacgttt acccttcgat aatccgcttc gcccgatggt caaagctagg
     2581 aacgcttatt tagaatcaga ggggatcaat cctttttacc aggaatccgt acctaaagca
     2641 gctttgaagt tacgccaagc attaggtcgc ttgataagga cggagacaga tcgtggcgta
     2701 ttgcttattt tggatcgaag actagtcact gcaaaatatg gcgcacggat catccatgca
     2761 ttaccgaaaa aagtaccgat caaagagcta gctatggagg aaatctatca ggatatccat
     2821 gaattcttaa agaaaaatga agaaggaagt tagaggagct gtcttttttc ataattaaaa
     2881 tctgctataa tgaaaaggaa tgaagataaa ggagcgacga cccttgaaga aaaaacagac
     2941 aaaggaaaaa acgattaatc gcgtgttact tggcttgatc gtatttttat tagcggtcat
     3001 cgtgatcagc tcgatttttt acttgcgttc cagtcgtccg gcggtgcagg cgaagaaaga
     3061 agcagtagaa atcgcaaaga aatatgcaaa tcttgaggaa accgatcatt tttattggtt
     3121 tactcgaaag aagacatact tttcactgac aggtacaaat gataaaggaa aagaaatcgc
     3181 cgttatcatt cctaaatctg gtgaaaaagt aaaagtattg gatcaaacaa agggactgac
     3241 tgaaaacgaa gcaaaacaga caattgctgc ggcccatcct gaaatccaaa tcgaaaaagc
     3301 tgctttaggg atgtatgatg atcagcctgt gtgggaagta gtaggaaaag aaacaaatgg
     3361 gaaactgaat tattacctga ttggctttga atctggagat gaagtcaaga caatcaaggg
     3421 aatttaaaag gtaaaggaat gaatgggatg gagttatcaa aaagaacaaa acgattagaa
     3481 ccgtctgtca ctttggcagc tgcggcaaag gccaaggcat taaaagcaaa aggcagagat
     3541 gtgctgagtt tgactgtggg agaaccggat tttgcgacac cagaaaacat ccaagaagca
     3601 gcaatcgaag caatccgcaa tgggaaagct agctattata caccaacagc aggaatccca
     3661 gagcttcgac aggcaatcgt tgattatctg aagaaaaact atggacttgt ctatgaacct
     3721 tcacaaacag tcgtaacaga cggagcgaaa tttgctttgt ataccctttt ccaaacgatc
     3781 cttgacccgc aagatgaagt catcattcct gtgccttact gggtcagtta cggtgagcaa
     3841 gtaaaactgg cagaaggact tcctgtcttt gtgaaagggg aagaatccaa tagctggaaa
     3901 gttactgttg aacagctaga acaagcacgt tcagaaaaaa caaaagccgt aatcatcaat
     3961 tctccatcga atccgactgg aatgatttat agcaagaatg aactgcaggc aatcggtgaa
     4021 tgggcagtaa aaaatgatat tttgatagtt gctgatgata tttatggccg tttagtttat
     4081 aatggcaatg agtttactcc aattgcaacg atttcagaag caatcaaaaa tcagaccatc
     4141 atcatcaacg gcgtgtctaa aacatatgcg atgacaggct ggcgcattgg ttatgcggtg
     4201 ggaaatcctg tgatcatcaa tggaatgatc gctgttgctt cccaatcaac tagcaatccg
     4261 acagcagtca gtcaatatgc agctgttgaa gcattgaaag gtgagcaaga tacagtagaa
     4321 gaaatgcgga acgcctttga agaacgcctg aatacgttat atccgcttgt ggcagaactg
     4381 ccgggtgttt ctcttgaaaa gccgcaagga gctttctatc tttttcctaa tgtaaaagaa
     4441 acgctacgca tgtgtaagta tgaaaatgtc acacaatggg tagaagatct tttagaagaa
     4501 acaggggtag cgcttgtgac tggcgaaggc tttggggcac cagaaaatgt ccgcatgagt
     4561 tatgccactg atcgcgctac gttagaagaa gcggtacgca gaatcaagca atttattgag
     4621 agtaaaagtc aaaattaatc ctgaagaaat tcaaggaata taaaaggaga gtcatacgtg
     4681 gaacaaattc aaatcatcga ttcgaaaaac catgtgggag aaacagtaac aatcggcgct
     4741 tgggtcgcaa ataagcgatc aagcggaaaa atcgcatttt tacaattaag agacggaaca
     4801 gcctttttcc aaggtgtagt tgtaaaaagc gaagtgtcgg aagaagtttt ccaaacagct
     4861 aaaaatctac accaagaaac atccgtgtta gtaactggag aaatccgtga agacagtcgt
     4921 tcaaaatttg gttatgaaat cggaattaca aatgtggaag tggtaagcga gagccacgat
     4981 tatccgatca caccaaaaga acacggaaca gatttcttga tggatcatcg tcatttatgg
     5041 ctgcgttctt caagacaaca tgcgattatg cagatcagaa atgaaatcat tcgtgcaacg
     5101 tatgaattct ttaatcaaca tcattttgtc aaaatcgatc cgccgatctt gactggttct
     5161 gcaccagaag gcactacgga tctattcgaa acaaattact ttgatcaaaa agcttattta
     5221 tctcaaagtg gccaattata catggaagct gctgctatgg ctttcggaaa agtcttttca
     5281 ttcggtccga ctttccgtgc tgaaaaatca aaaacacgac gtcatttgat cgaattctgg
     5341 atgatcgaac cagaaatggc tttcactcat caagaagaaa gtttagaagt acaggaacaa
     5401 tatgtagcat tcttagtaca aaatgtttta gatcattgcg aatatgcatt agatgtacta
     5461 ggacgagaca aagaagtctt aaaaacttat acaaaattgc cattccctcg tattacttac
     5521 gatgatgcaa tcgaattact gaagaaaaat ggatttgatg acatcgaatg gggcgaagac
     5581 tttggttcac ctcatgaaac gttcattgcg aattcattcg aacagcctgt ctttatccta
     5641 aactatccaa aagccatcaa gccattctac atgaaaccac atccaacaag agaagacgtg
     5701 gtaatttgtg cagatatgat cgcaccagaa ggttacggtg aaattattgg cggaagcgaa
     5761 cgggcaacag actatgattt cttgctagaa caaatccgta atcacggttt ggatgaaaaa
     5821 gaatattcat ggtatcttga tctacgtaaa tacggttctg ttccgcactc tggttttggt
     5881 ctaggccttg aacgaacagt cacttggatt gctggtatcg accatgtacg tgaagctatt
     5941 ccattcccac gtttgttgaa ccgaatttat ccataagagg tgatgaaaaa gctgttctgc
     6001 atcaagtaat tagaacagcc aagcaaaaac agctgttcat gtttttgaat tggcgggact
     6061 tattaccgca gatgtaagct tttgaatacc gtttagtgga gaggttgtga aaaagctgtt
     6121 tagtttcgag tattaagaaa aattttgtgg aaatggcttt ccacattttt gcataaattt
     6181 ggcttaatac cgagaaactg gcttttgaac accgtttaag gaagaggttg tgaaatcagt
     6241 gccttgacct gagtattaga gaaacaaaac ggaaaaatag cttctcatat tttaccgttt
     6301 ttttagctaa tatcgagggt cagctgattg aacaccgttt atatcaagag gttgtgcgcc
     6361 tgccgttcag ctccgagtca caaggagtaa tttcaaaaaa cagtttttca tgtttgatga
     6421 ctattgtgac ttgtgaccga ggagttggcc ggagagcaac gtttatttaa aaaaaagaac
     6481 tgcgaactct cgcagttctt tttttaaaat acgggataaa ggaagtttcc agcagacaag
     6541 agaagaaagc aaaagaaatt gctatttgat tcacaaggtt ctataacgaa atcgcaatca
     6601 atcaaacata tacaaagaag gttattcaaa tggaaatctg atttgatgat gcttctgctg
     6661 ttgaaataaa gcagcatcta gtacctgaaa acaaactaat agtaacattc gaagatggag
     6721 taggtccgta ttttcagcat gcaatgatcc atatgtaagt ttaattcatc cttgaaatca
     6781 tcggaccaga taaagttatg accaatctat cccaagcaat ctagaagaga tattagtaaa
     6841 agaacattta ctggaagtct tagatgagcc tataaatata tgttctgatg gcgtcatcag
     6901 tggtctgatt gacggaagct taggtgtgat cgatttctct ggaccaaaca acgaaggaat
     6961 caaggggaat ccggcacgtt aagaaaaaaa gtataaaaaa ctagacactt gatgagcgtt
     7021 cattcaattg attgaaggga ctaggctggt gtcatttttt atacatatat tttctctttc
     7081 gtcttgatag ggcttatggg ataatataag gaagaaaatc atcgttttat tcaaaaagga
     7141 gtttttgaca aatgaaaaaa cgtatattat ttatcggtga cagcattaca gacgcaggaa
     7201 gagaccgagc agaagcaact gatctaggta aaggttatcc acttttgatt gccggaaaac
     7261 taatctccca atatccaacg atgaaatttg aatgcatgaa ccgtgggata ggaggagaca
     7321 aaattgcaga tctgcaagta cgctggcaag aagattgttt agctttacaa cctgactatg
     7381 tatcgatttt gatcggaatc aatgatacct ggcataatac tagcgatcag gctatattcg
     7441 gaactgagaa atctgcgcag caatttgaat ccgtttatcg aaaattacta aatgaacttc
     7501 aaacagcgga aatctgcaac atcatcttga tggagccgtt tgtacttcct ataaccgacg
     7561 accgtcatgg ctggcgtaaa gacttagacc ctaaaatcca aattgttcgt aaacttgcta
     7621 aggaatatgg cgcattgctg gttcctttag atggcatatt caatgcttta gggattgctt
     7681 atggtttttc gaaatataca agagaagatg gggtccaccc aacgattggc ggacatgaga
     7741 tcattgcaca aagctggata gatgcaattg ccgcagtcga tttttttagt ggagataaaa
     7801 atgagcgaaa gaaaagtgag aaatgatgga aatacggtgg gcagtaaaag aagatttacc
     7861 tcaaatcaat cagctgatta ctgatcatcc agaatcattc gtaaaagcaa aaacggcagc
     7921 tgacctaaat gataagaata aactattgct cgttgctgta acaaaagaaa aacggattat
     7981 cggatttgcg caagcggacg tgcaacagtc agaagaacgg gtggatcagc gaccatcagg
     8041 aaaactaatc caacacacat tagtaacaaa agaaaatacg gaagaaatcc aaaaaaaatt
     8101 tgcagaatac ttgatgctgg aagctaaaaa aagaagaata aaatcaattt ttgcccagtt
     8161 ttaatcaaaa acagtaatag aaggagaaat acaatgagtt gtttaggaaa tgtcatctgg
     8221 tttatattcg gcggactaat cggtggtctt agctggttag cagcggggat actttggtgt
     8281 atcacgatca taggtatccc aatcggtatg caatgtttta agatcgcctc cctttctttt
     8341 gcgccttttg gcaaagaagt aatttatcac cagtctggca tgtcattgat cgttaatatc
     8401 atctggctga tcgtttcagg cctaccaatt gctctcgcac atgtcgtcag cggcatcctt
     8461 ctatgtatca cgatcatagg gatacctttt gccaagcaat ctttcaaact tgctcgattg
     8521 gcgttgatgc cattcggtgc agaaattgta taaaaagaga ataataaagg ttagaaaacg
     8581 catagagtca ggtgggatac atgtatttca cctgactcta tgtgttttcc tgtataagtt
     8641 ctcccatgtg acttttacgt cttttattta tatgggatta ctgtttatgg aaagttgaaa
     8701 taattttcat taaagaactc tttctatatt tttgaaagcg tagtggagct tcgaatatct
     8761 gggaaggaca ttttgcaggg attgaaaaaa gatattgtcc tatcgaaaaa ttaatgaatt
     8821 tagacaaatt gccagcaaca ggcttcaaac ttagttgcta tcctgtcaag atcaagaagg
     8881 caagtgcggg ttggataaga gcaggggcaa tgatcgaaga gaaaaagaaa gaataagcca
     8941 aaacatctgt atggaagaaa ttttccgatg cagatgtttt tcattttttc tttcggatac
     9001 cttgacatat gaataaccat tcatttataa ttagttatga agagatgttc atatgttgag
     9061 gcgaggaggt cattaaaatg aaaagttaca gattagaagg tttagattgt gcgaactgtg
     9121 caatgaaaat cgaaaaagga gttcagaaga tcaatggagt caaggaagca acggtaaact
     9181 tcacttctgg aaaactaacg attgacgcag aggaagatca tttggctacg attgaacaag
     9241 aaacaaaaaa agtagtgaag gaattagaac cagatgtcaa agtaacagaa atagataagg
     9301 aaaaagtatc tgaacatgga aatgaaaaag agagaaatac gctgttccgg attttatttt
     9361 cccttgcagg tatcgcttta ttgctattat ttgattttaa tgaaccaatt cgcttgatag
     9421 ggtatttatt gatttatctg ctgataggat atgatgtagt gaagaaggca gtgatgaata
     9481 tcgtcaaagg aaaaatcttt gatgagaatt ttcttatgtc tgttgctacg attggcgcta
     9541 tgttgatcgg tgaatatcca gaagccgtag cagtgatgct attttatcaa atcggcgagt
     9601 atttccaagg attggcagtc agtcattcaa gaaaatcgat tcgcgaattg atggcgatcc
     9661 gtccagaaac agctcacgtg cagacgacgg aaggattgat gaccgtgaac cctgaagacg
     9721 tgcagatcgg tcagtttgta cttgtgaagc ctggtgaacg tgtaccgtta gatggaacga
     9781 tcatcgaagg agaatcatta gtcgatacgt cagcacttac aggagaatca gtccctaaat
     9841 ctgtatatgt tggtgaaaca gtattaagcg gatttatcaa taaaaataaa ccatttttag
     9901 ttcaagtaga aaaatcttat gaaaattcta ctatcagcaa attattagaa ttagtagaaa
     9961 atgccagcag taaaaaagca ccagctgaaa atttcattac taaattcgcg cgctattaca
    10021 caccagtcgt tgtaggattg gccgttttat tagctgtttt acctccactt gttgtttctg
    10081 gagcggcgtt ttctgaatgg atctatcgag cattgacatt tttagtgatc tcatgtccat
    10141 gtgcattagt gatttccgtg ccacttagtt tctttggcgg tatcggaggc gcaagtaaaa
    10201 tcggtgtatt agtaaaaggt tcaaattact tggagttgct tgcgcaaacc gagacagtgg
    10261 tattcgataa aaccggcaca ttaacgaaag gtgacttttc gatccagacg atcgatacaa
    10321 aaatcgatcc aaagaaattc atgcaatata ttgcgagtgc agaacagttt tctacacatc
    10381 cgattgctca atctgtttta gaaggctacg caggtccgct tttaccaaca actaatatcc
    10441 aggaatttgc aggtgaaggg atcttagctg aagttgatgg caaacaagta ttagcaggta
    10501 atcacaaatt gatggaaaga tttgaaatct cttttccttt aagtcaagaa atcggtacat
    10561 tgctttatct agcgatcgat caaagttaca gtggttatct tgttattgca gataccttga
    10621 aagaagatgc cgtagacgca ttggttcaat taaaacaggc tggtgtgaag aacacagtca
    10681 tgttgacggg cgactcgaaa aaaattgctg atcatatcgg gaagcaagta ggcgtggaca
    10741 agatatatag cgaattgctt cctgaagata aagtgcagcg gttagaggaa atcttacaaa
    10801 gaaacaataa aaagaccgca tttgtcggag atggaatcaa cgatgcaccg gtgctagcaa
    10861 gagcagatgt aggaatcgca atgggtggat tgggaagcga tgctgcaatt gaagcagcag
    10921 atgtagtcat catgaatgat caaccatcga agattgcaga agcaatccat ttagcaaaga
    10981 aaacattgaa aatcgtcaaa caaaacattg tctttgctat tgggataaaa attttggtac
    11041 ttgccttagg tgcttttggt tttgcatcaa tgggagatgc agtatttgca gatgttgggg
    11101 tgacagtatt agctgttttg aatgccatgc gaagcttgca tgtaaaaaaa gaggacgaat
    11161 gaacgtcctc ttttttttat atccttttct taatgaaaaa tgtaaagaaa atagatagga
    11221 ttataaaata actaatgttt ttaattttgc agaaaattca aaatagcttg aaattcaata
    11281 aggtcaatga aataaaagaa aataacttat ttttttgatg gaaaaaatat gaaaaaacga
    11341 tgtgtattaa ataaaagaat acacacataa aataacactg tcttgtaagt ttgttaaaaa
    11401 ttgaatatga ttacatctgg ataacaaatt tttatcataa aggagagaac gaatgaacaa
    11461 caaacaatta cgctggttta ctgttgcgtt aattgcattt aacatggttt ggggcatggg
    11521 gaatgtagtt aataactatg ctcagcaagg agtatcagtt gtcacctctt ggctattgat
    11581 tttggccatt tattttattc catacgcatt gatcgttgga caattaggat cagccttcaa
    11641 ggatagtaaa ggaggcgtca gttcatgggt cgaaaatacg acatctaata aacgactggc
    11701 atactatgcc gcttggactt actgggtcgt ccatattcct tatttggcac aaaagcctca
    11761 agcaattctg attgctggtg gttgggcagt ccaaggaaat ggaaatatcg taaatacgat
    11821 gtcagtacaa atggtagcag ttatttcact tattatattt cttgcctttt tatatttatc
    11881 aacaaaagga ctttctactt tgaaagtgat tggcggactg gccggaacag cgatgttcgt
    11941 catgtcgatc ttatttattc ttttagctgt aacggcaccg ttcatcaatc caacgatgga
    12001 atttgccaca ccacatatga atcaagtgaa aacatatatt ccaacatttg atttcagtta
    12061 tttcacaacc gtttcgatgc tagtatttgc agttggaggg gcggaaaaaa tatcccctta
    12121 tgtcaactct acaagaaatc ctgcaaagga atttcctaaa gggatgatct tcttagcagc
    12181 aatggtaggg atttgtgctg tattaggctc agtcgcaatg ggtatgctgt ttgctagtga
    12241 taatattcct aaagacttga tggcaaatgg ggcatacagt gcttttcaaa ttttaggtaa
    12301 ttactatggt atcggtaact cattgatgat catttacgca ttgacaaaca tgattggaca
    12361 aatctccgca ttagcctttt caattgatgc accactaaaa atcttgttag cagatgcgga
    12421 tccagaattt gtaccaaact ggctacgtaa acgtaatcgt aaaggtacat tggtgaatgg
    12481 ttatcttctg acaggtatac tagtaggaat cattattttg ttgccgattt ttggtatcaa
    12541 agatatgaat gaattagtaa aatggttgac gaacttgaac tcagttgtca tgccaatgcg
    12601 ttacctatgg gtgttcttgg catatatgat gttgaataaa gcttataaga aattcaattc
    12661 tgaatataaa tttgttaaaa atccaaaaat cggttttatc tttggtgctt ggtgctttgc
    12721 cttcactgcg tttgcatgta tcctaggaat ggtgccaaaa gtagattata ctgctgatcc
    12781 taaagcatgg tggttccaat tatttacgaa tatcatcaca ccaatcgtct tgatcttatt
    12841 aggtatgatc ttgccagcaa ttgctcgtcg agacaaaaac aaagaaatca ctgaaccaga
    12901 aacagtggtt cagccttaaa atagaaatac ttgttacaag aggttgtgac agaagtggtc
    12961 agcttcaaga aataagaagg aatttacgaa aattgcttct caaatttttg tgaagtttcg
    13021 gcttgttttt cgaagaagct acttctggaa caccgtgaat cagaggttgt gaaaaagctg
    13081 tcctgcatca agtaataaga acagccaaac aaaaatagcc cctcatattt ttcgtttggc
    13141 tgggcttatt accgcagatg caagctttgg aacaccgttt aaggaagagg ttgtaaaatc
    13201 agcgatttga cctgagtatt agagaaacaa acggaaaaat agctcctcat attttgccgc
    13261 tttttgagct aatgctgaag gtcagctgat tgaacaccat ttatt
//
LOCUS       DAZKKW010000070        13254 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.071, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000070 DAZKKW010000000
VERSION     DAZKKW010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13254
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.071"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(118..1227)
                     /locus_tag="ACG8PO_002022"
     CDS             complement(118..1227)
                     /locus_tag="ACG8PO_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFQ0207324.1"
                     /translation="MKNKILLFLTLICTLVLTIYPASQLYHQFKTKENVAEIQEKIEK
                     KQTPSKEEVAKKVEQIKSNSGNQGLDNTQKTDKGVDNTQETDGGSFDIREAEGKVNPY
                     TGDVFSKNQTKDFEPSTYSGAVGYVYIPKINVLELLFLGASQEHLYRGVAQMSATSLP
                     LSGKGHQTVIAGHRGGYTSPQFLYVDQLVAGDHIYIDYLGEKAVYTVFRTDVIRNYGQ
                     NSTLTDIPDLDMLTLYTCHPYPQNYDRLLVRSLRDEKAKYITPQVTVGNTAYADAIDS
                     ESVSDKPETVTSSNDLFEDVDRANKELDGNNDLNIPEQGSVEMQDRTFAEKASSIFTG
                     ITNKHQKMLLVIWTVLGALASLKVLILLIKEFRKA"
     gene            complement(1217..2074)
                     /locus_tag="ACG8PO_002023"
     CDS             complement(1217..2074)
                     /locus_tag="ACG8PO_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFQ0207325.1"
                     /translation="MKKKNIKLIILKIGMLLCLGVGLYPVITQAYTYYQTQQTLNSYK
                     EKYDEASEKENEEVFKRYSEVNRKRKDTEQTSVTIPEDNNNIPSNGNLQKDKLGELVG
                     EILIPKINVDLPIYDGVNDFQLSAGAGILTGTDYPTGGIGNHSVISAHSGIPNKKYFT
                     DLELLKLGDKFFIDISGKKLAYQVDRIKTILPTEFEDLLPVAGKDYVTLMTCTPVGDN
                     SHRLLVRGVSIPYQKSIVEKEKKKEDLKVKAQMNWYPIVVVGVLLIIAVIVIIKKIVA
                     KRRGMKNEE"
     gene            complement(2149..3984)
                     /locus_tag="ACG8PO_002024"
     CDS             complement(2149..3984)
                     /locus_tag="ACG8PO_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFQ0207326.1"
                     /translation="MKSKKIFSNLMLFGTLLGVGLGASSISSSLGLGGQVAYAQQNYN
                     YQGTGTIHIHKVTNTNGDGNPVSDIDNKGKEIALPNGVKPLEGVEFSYVKLTKDEAKS
                     LGWTDSDTTKGQHIDDAKARLLLNKKYSEVSRMKTDSQGNIDIPIQVNTDDLTDNLYL
                     IVETGTVKGVVEEAYPMIVNVPELNGSDDQYFNYDVHVYPKNYFQLNSAELDKKDEDG
                     KALAGTVFELRKDDGSDQVVGTYKTDSTGKLTVDGLELGDYYFLETDLGTDANRTTYL
                     LDGKKFKFSVKRGETAKVVLDVTNYKRPVITKVIDKETVDTGEDVQYTLKPSIPGNIG
                     NYEKYVVKDVLGAELTYDSSEVIEGMTLDTDYSIERDGQTTNYVFTESGMDKLQKQFD
                     AGDRFFTILMNAHTNSKAKMDTPLKNDVSTIYNNGYEPEDETPVDHAYTIIGDHQFIK
                     TDGDTSKALSGAKFVVKNKIDPNKVTYLKQDPTTKAVTWIADKSKATEFVSGSDGKFQ
                     IEGLQYTYDVDFDKQGNPTITDKKINDYALEEVVAPSGYTPTRNDVPFVVSNDDASSE
                     VTDIKNLKTPETVRTGGIGTVVFLVVAGVAGTGLYVVYKREQHKA"
     gene            complement(4033..6423)
                     /locus_tag="ACG8PO_002025"
     CDS             complement(4033..6423)
                     /locus_tag="ACG8PO_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen binding domain-containing protein"
                     /protein_id="HFQ0207327.1"
                     /translation="MKHEKTIILKSKIIYCFATLVMVLSTLIMPFSAIRAEADVTKPT
                     SAKFTETGVKTDGYIRVNMPNHPNEWMISSQFKDSHGNIGYCMDSELPSPTGSGAGSL
                     KYKGAGSDEFYRMFKGGFPSKTAKELGAGNDTEAWYATQLVSWVLAGNFKVSQIVWSH
                     PNHTAAETARVKKAFEKIYDYAKNGKDTPNTEFSITASKTADEGKYHTFTYKTASNKT
                     GNAKLTFTSAKPAGMKIYDADGKEITNNTVKLNSSFTIKVPVTTPSGTLSFKGTANVS
                     TTNPFTFDGRGVYQDAVVMITTSKTKDSKSLSAKWTRAVGQVTVKKVDQDGNALKGAE
                     FTLTDNNNAKSVKTTGDDGLATFDLQQGLTYSLEETKVPEGYQGSFKKDGITVNTDGQ
                     EFSYTATNTIKKGKIKVKKVDQDGKPLANAEFTLTDDSSQTQAEKTDENGLASFDIVA
                     NKTYSLEETGVPTGYTGAFKQEGITVKDDGQTFEYTATNTLKKGVVKVHKVDQDGKPL
                     ANAEFTLTDEKGATQVEKTDENGLASFNIVANKTYSLEETGVPVGYTGAFKQEGISVK
                     DDGQTFEYTATNNLKKGVVKVHKVDQDGKPLADAEFTLTDEKGVKEVKKTDATGLATF
                     NIIANKTYNLEETKVPTGYTGAFKQEGITVKDDGQVFEYTAKNTLKKGVIKVHKVDDQ
                     GKALANAEFTLTDDKGVKVVQKTDRNGLATFNIVANKTYKIEETQNPNGYTGKWSKEG
                     ITVADDGQTFEYTVINHLVPETVRTGGIGTSAFLLVFLIAGLSMVLLYYREQHKAK"
     gene            complement(6693..7940)
                     /locus_tag="ACG8PO_002026"
     CDS             complement(6693..7940)
                     /locus_tag="ACG8PO_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HFQ0207328.1"
                     /translation="MNMKKNSLGMVLFASAVLAGLSVPVTSHADEWTANTPDQIQITT
                     GQSSYAFKKGDTIWSTVEKLKSLGYKTTVAEFAELNNVDLAKGEQRTIPIGRTFNISK
                     DTLTVKEADGEVVSQTVQKDKPDPVENTEQENKKSETTKESEPTANTSDKTETSASSV
                     DGASSSNTNNSTSEKEASQGTSESSTTEADTGTSTNNSQSTSNDANESTSTSSSDQSQ
                     TGSSDETDSSTSPSKPEQPTNVVLGVPFISQGNTMLCEGTSLLEALHYKGVATNQDLM
                     TFVKSMPLSPDNNPNNGFSGEWRHNVDGTYQGMNPAPVVSWGVANGGNVVDISGSSVQ
                     GLKDEIVNGNPVVAWVTYAFEPAQHKSMFWGDAIWNRHVVAVDGFKDGFYHVVDPVFG
                     ASWINAGSFETAYNVSQLAVSVR"
     gene            8332..8748
                     /locus_tag="ACG8PO_002027"
     CDS             8332..8748
                     /locus_tag="ACG8PO_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0207329.1"
                     /translation="MNKKRKLSVLITLISILTILSGILSLKIFNQINTSSNAKDGIVV
                     ENTSNSKETGTTTKKDKSAEITTKNYLIKTVYFDERKTAILKDEEGSELKLPITVSDK
                     FNGTFSLQTAEGSPDNLKVGQTIILGKNDELYSFVK"
     gene            complement(8765..9502)
                     /locus_tag="ACG8PO_002028"
     CDS             complement(8765..9502)
                     /locus_tag="ACG8PO_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0207330.1"
                     /translation="MADRKLEKLLEETWNPKEFSEFFMENFETDLAVIVKDALREQGY
                     PETANYININFTLYTENKGTWDFWATLANKELSDKSDTGIRNFFESNRDDYMYANNQN
                     KLNFRVEFDETPEEFIERQPPKENVAKVLEDRWNSDEIVSTISELGGQYEPLVEAVRE
                     ELRLNKFPDVQNIDVSQIEINVKITNKLDYGSWADIALEKYIYSTLKEFIENRMDIMY
                     LQHPQYLNFGVEIATPLEEWKMEQGLD"
     gene            complement(9924..10487)
                     /locus_tag="ACG8PO_002029"
     CDS             complement(9924..10487)
                     /locus_tag="ACG8PO_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0207331.1"
                     /translation="MQNHEITTAKKAIGILSIIVILCCVVLFQKKNAYDNNVKELEDL
                     KSKQIEVVKNNQKVKAMQEAQYKELGNAEIKEQSRKFFNLFYNWNSWEKYTKNMKEIQ
                     KEFPQIVKSSPVDISGTMVGTGNSPISSYTADYYTTNQKGKMVELITQARNTPTTATT
                     TIWRGVTSIGENQLFYLEDLDSYVRAE"
     gene            complement(10474..11001)
                     /locus_tag="ACG8PO_002030"
     CDS             complement(10474..11001)
                     /locus_tag="ACG8PO_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0207332.1"
                     /translation="MNSKVKDTIIISLLSGMLLALLVGILFFNKQNQDIEAEKHSVHI
                     EDKLTFFKEVKPNEVDGLRKDLENDIQEMLNGSVQNKFADQSGEEFLRKLFIVTGKPP
                     ITDKSSDKEKIEYYKPFTFKLTNFGIKTSSSGLYEVLTDFEVQYNGKKVYDTTLTITL
                     NNDKKVTGGAFYAKS"
     gene            complement(11024..12409)
                     /locus_tag="ACG8PO_002031"
     CDS             complement(11024..12409)
                     /locus_tag="ACG8PO_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tip lysozyme"
                     /protein_id="HFQ0207333.1"
                     /translation="MKKSKKTFGTLLIAVMQIIGITASLITINPQVTHADGIATYDAQ
                     FNCSTEGSQGSSGSGKDSDGNETGSGAVSGDVAKNIDDIYKFMNGEYGLSGATIAGIL
                     GNWKQESGNDPRTVEGYYGDGQREVDFIKQFTDGLMGIGYGQWTAGRHTALVNWAKKN
                     FGGAWWTTEAQLDFMFKGDGGFVAILKDYALNASDDVVANAVAFHKNWEISADSESAV
                     VAVRGGNAKEIWEYMKSKGMDGAKDVSKINKIAGGTSTGGGSNNSSSSASGTVEDACA
                     TTDDANSGSGSMGKEGKINGKKGQVIANMTYEEALKKYGKAITLPKFKEPDWSTSPFN
                     GIYQGQCTELTWAYMSQLYDGNQPTTGNGGYVWESYKSAGAKITNKPIVGYGFSASDG
                     WLWAVGDAGHTGVVIAVFEDGSFLCANFNVPPTQAPTRGVSVTLIDGVDGSDNVHFFS
                     GVGKAKFEIEK"
     gene            12492..12983
                     /locus_tag="ACG8PO_002032"
     CDS             12492..12983
                     /locus_tag="ACG8PO_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0207334.1"
                     /translation="MTDKTGNLPKLAPKTSQTKSKKDEKIKNAVKKKSVKESSSLLLD
                     IPMSTFDEILDDNTDETSVKIASHVGMNSQNDSFYHLLNSLRELEDKKNQVIASKAPD
                     LTTDLDAEIIQVKQDERSVKSLLKTYFDGFDKELAPYSQAILTKPIQAPHISISEQKK
                     SDD"
BASE COUNT         3758 a         2672 c         1967 g         4857 t
ORIGIN      
        1 attaagatca gttttgattt tatttaaaag tgaatctagc tctattcaaa ataaaaaaga
       61 gatcaataat agtatcaatc cctttttact gtactctatc aatttttata atcttcatta
      121 tgcttttcta aactctttga ttaaaagaat aagaaccttt agacttgcta atgctcctaa
      181 tacagtccaa atgactaaaa gcattttctg atgtttattg gtaattcctg taaatattga
      241 acttgccttt tcagcaaacg ttctgtcttg catctctacg ctaccttgtt ccggaatatt
      301 aagatcatta tttccatcta attccttgtt ggcacgatca acatcctcaa ataaatcatt
      361 agaagatgta acagtttctg gcttatctga aacggattca ctatcaatgg catcagcgta
      421 tgcggtattg ccaactgtta cttgtggcgt gatgtatttt gctttttcat ctcttagact
      481 tctaactaat aaacgatcgt agttctgagg atatggatga caagtataaa gtgttaacat
      541 atctaaatca ggaatatcag tcagtgttga attctgaccg taatttctaa taacgtctgt
      601 tcggaaaacc gtataaactg ctttttcacc aagataatct atataaatgt ggtctccggc
      661 tactaattga tcaacataaa gaaattgtgg tgacgtataa ccgccacgat gacctgctat
      721 tactgtttga tgacctttac ctgataaagg tagactagta gcactcattt gtgccacacc
      781 tctatataaa tgctcctgtg aagcgcctag aaacaaaagc tctagcacat ttatttttgg
      841 aatatagaca taaccaaccg ctcctgaata ggtagatggt tcaaaatctt tcgtttgatt
      901 ttttgaaaaa acatctcctg tgtatggatt gacctttcct tcagcttcac gaatatcaaa
      961 tgatccaccg tctgtttcct gagtattgtc tactccttta tctgttttct gagtattgtc
     1021 taatccttgg ttaccgctgt tcgattttat ttgttcgact ttttttgcca cttcctcttt
     1081 tgaaggagtt tgttttttct caattttctc ttggatttcc gccacattct cttttgtttt
     1141 gaattgatga tagagctgac ttgcaggata aattgttaaa accaaagtgc aaataagagt
     1201 caaaaaaagt agtattttat tcttcatttt tcattcctcg tctctttgct actatttttt
     1261 taataatgac tatcacagct attattaata aaacccctac aactacgata ggataccagt
     1321 tcatctgcgc ctttaccttt aaatcttctt tcttcttttc tttctcaaca atactttttt
     1381 ggtaaggtat gctaacccct ctaacgagta acctatgaga attatctcca acaggtgtac
     1441 atgtcataag tgttacataa tcttttcctg ccacaggaag caaatcctca aattcggttg
     1501 gtagaattgt ttttatccga tctacttgat aagcaagttt tttccctgaa atatcaataa
     1561 aaaatttatc accaagtttg agcaactcta aatctgtaaa atatttttta ttaggaatgc
     1621 ctgaatgagc tgatataaca gaatggtttc caatcccacc agttggataa tcagttccgg
     1681 ttaatattcc tgctcctgct gatagttgga aatcgtttac accatcataa attggcaaat
     1741 ccacattaat ctttggtata agaatttcac ccactaattc acctaattta tccttctgaa
     1801 gatttccatt tgacggaata ttgttattat cttcaggaat cgtgactgag gtttgttctg
     1861 tgtcttttcg tttcctatta acttctgaat atctcttgaa gacttcttca ttctcctttt
     1921 cagaagcttc atcatatttt tccttatagg agtttagtgt ttgttgagtt tggtagtagg
     1981 tataagcctg agtaattaca ggatatagac ctactcctaa acatagcaac atcccaattt
     2041 ttaagatgat caacttgata ttcttctttt tcatgctcat acctactttt cacacacttt
     2101 ttaaaaagaa accctagagc caaaaacact ctagggaatt ttatttcatt aagctttgtg
     2161 ttgttctcgt ttgtagacaa catatagccc tgttcctgcc acacctgcaa ccactagaaa
     2221 tactactgtt ccaattccac ctgtacgaac tgtttcaggt gttttaaggt tcttgatgtc
     2281 tgttacttcg ctagaagcat catcattcga taccacaaat ggaacatcgt ttctagttgg
     2341 tgtatatcct gaaggagcaa caacttcttc aagagcataa tcattgattt ttttatctgt
     2401 gattgttgga ttaccttgtt tatcaaaatc tacatcatag gtatattgca aaccttcaat
     2461 ttggaactta ccgtctgatc cggaaacgaa ttcagtagct ttagatttat ctgcaatcca
     2521 agtaacagct ttcgttgttg gatcttgttt taggtaagta actttattcg gatcaatttt
     2581 gttcttcact acaaattttg ctccactcaa tgctttgctt gtatctccat ctgttttaat
     2641 gaattggtga tcaccaatga ttgtataagc gtgatcaact ggtgtttcgt cttcgggttc
     2701 atatccattg ttgtagatcg ttgaaacatc attcttaagt ggtgtatcca ttttagcttt
     2761 actattagta tgagcgttca tcaaaattgt aaagaagcga tcccctgcat caaattgttt
     2821 ctgaagctta tccatacctg attcagtaaa gacatagttt gtcgtttgtc cgtcacgttc
     2881 gatactgtaa tcagtatcta aagtcattcc ttcgatcact tctgatgaat cataagttaa
     2941 ctctgcacct agaacatctt tgacaacata tttttcgtaa ttaccaatat taccaggaat
     3001 tgatggtttc aatgtatatt gaacatcttc gccagtatca acggtttctt tatcaattac
     3061 cttagtaatt acaggtcgtt tgtagttggt aacatcaaga actactttag cagtctcccc
     3121 acgtttaacg ctaaatttga attttttacc atctagtaaa taggttgttc tattagcatc
     3181 tgttcccaaa tcggtttcta ggaaataata gtcacctaat tccaaaccat ccacagttaa
     3241 tttacctgtg ctgtctgttt tataagttcc aacaacctga tcagatccat catcttttcg
     3301 caattcaaaa acagttccgg caagagcctt tccatcttcg tcttttttat caagctctgc
     3361 tgagtttaat tggaaatagt ttttaggata gacatgtaca tcataattaa agtattgatc
     3421 atctgatccg tttagttcag gtacgttaac aatcatagga tacgcttctt caactacacc
     3481 tttgactgtt cctgtttcaa caatcaaata caagttatct gttaaatcgt ctgtatttac
     3541 ttgtataggt atgtctatat tcccttgtga atcagtcttc attctactta cttcactgta
     3601 ttttttgttt agtaataatc gtgctttagc atcatcaata tgttgacctt ttgtagtatc
     3661 actatccgtc catcccaagg atttagcttc atctttagta agtttcacat aagagaactc
     3721 aacgccttca agaggtttta caccatttgg taaagctatt tctttccctt tgttatcaat
     3781 atctgagaca gggttcccat ccccattagt atttgttact ttatggatat gaatcgttcc
     3841 agttccttga taattatagt tttgttgtgc atatgctact tgccccccta gtccaaggct
     3901 agaagagatt gagcttgcgc ccaatcccac tcctagcaaa gtaccaaata gcattaaatt
     3961 tgaaaaaatt ttttttgatt tcatacgtca gccttctttt ttcatattta cttcttttcc
     4021 attaacactt ttttattttg ctttgtgctg ttctcgataa tacagtagca ccatactcaa
     4081 tcctgcaatt agaaatacaa gaaggaaggc tgatgtaccg attccgcctg ttcttactgt
     4141 ttcaggtaca aggtgattaa ttactgtata ttcaaaggtt tgtccatcgt cagcaacggt
     4201 gatgccttct ttagaccatt tccctgtata accattaggg ttttgtgttt cttcgatttt
     4261 gtacgttttg ttggcaacaa tattaaatgt tgccaagcca tttctgtccg ttttttgtac
     4321 aacttttaca cctttgtcgt ccgtcaaagt gaattctgcg tttgctaatg ctttcccttg
     4381 atcatcgact ttatgaactt tgatcacacc ttttttcaaa gtgtttttag ctgtatattc
     4441 aaagacttgt ccatcatctt tgacggtaat tccttcttgt ttgaatgcgc ctgtgtaacc
     4501 tgttggtact tttgtttctt ctaagttgta agttttgttc gcaataatgt tgaacgtagc
     4561 cagaccagtt gcatctgtct ttttaacttc ttttacacct ttttcatccg ttaaggtgaa
     4621 ttctgcatct gctaaaggtt ttccatcttg atcgacttta tgaactttta ctacaccttt
     4681 tttcaaatta tttgtagctg tatattcaaa agtttgccca tcgtctttaa cactgatacc
     4741 ctcttgctta aatgcacctg tgtaacctac tggtactccg gtttcttcta gactatacgt
     4801 cttgtttgct acaatattga agcttgcaag tccattttca tctgtttttt ctacttgtgt
     4861 tgcacctttt tcatcggtta aagtgaactc tgcatttgcc aaaggttttc catcttgatc
     4921 gactttatga acttttacta cacctttttt caaagtgttt gtagctgtat attcaaaagt
     4981 ttgtccatca tctttaacgg taattccttc ttgcttgaat gcgcctgtat aaccagttgg
     5041 tactccggtc tcttcaaggc tgtaggtctt gtttgctaca atatcgaagc ttgcaagtcc
     5101 gttttcatct gttttttctg cttgtgtttg ggatgaatca tccgtcaagg taaattcagc
     5161 attagctaat ggttttccat cttggtcaac ctttttaact tttattttac cttttttaat
     5221 tgtgttcgta gcagtgtaac taaattcttg tccatcggta ttaacagtaa taccatcctt
     5281 tttaaatgaa ccttgataac cttcaggaac ttttgtttct tctaagctgt atgttaagcc
     5341 ttgttgcaaa tcaaacgtag ctaacccatc atctccggtt gtcttaacag atttagcatt
     5401 gttattgtca gttagagtga actccgctcc ttttaaagca tttccatctt gatcaacttt
     5461 cttaactgtt acctgaccaa cagctcttgt ccatttagca ctcaaagatt tagagtcctt
     5521 tgtttttgaa gtagtgatca taactacggc atcttgatac acacctcgtc catcaaaagt
     5581 aaaaggattg gtcgtgctaa cattagcagt acctttaaat gacaaagtac cacttggtgt
     5641 agtcacaggt actttaatag taaaacttga atttaatttt acagtgttgt tagtaatttc
     5701 tttaccatct gcatcataaa tcttcatacc agcaggtttt gctgatgtaa aagtgagctt
     5761 cgcattacca gtcttatttg atgctgtttt atatgtgaaa gtgtgatatt ttccttcgtc
     5821 agctgtctta gaagctgtga tagaaaactc agtgttgggc gtatcttttc cattcttagc
     5881 atagtcataa attttttcaa atgctttttt tacacgagct gtttctgcgg cggtatggtt
     5941 tggatgtgac catacaatct gtgaaacttt aaagtttccg gctaaaaccc atgaaactaa
     6001 ttgtgttgca taccatgctt ctgtatcatt tcctgcaccc agttcctttg ctgtttttga
     6061 tggaaaacca cctttaaaca tacgatagaa ctcatcactg ccagctccct tgtacttcaa
     6121 gcttccggct ccacttcctg ttggggatgg caattctgaa tccatacaat atccaatatt
     6181 tccatgagaa tctttaaatt gacttgaaat catccattca tttggatgat tcggcatatt
     6241 aaccctaata taaccgtctg tttttacgcc agtttctgta aattttgcac ttgtcggttt
     6301 tgtgacatct gcttcagctc ttattgctga aaaaggcatt ataagcgtag ataacaccat
     6361 tactaaagtc gcaaaacaat aaattatttt tgattttaaa attatcgttt tttcatgctt
     6421 catctctcgt tactccttct ttataccagt taggcgaaga aaatgagcca cgatatgtcg
     6481 gtgtggtaat gaccttcgcg cccattccta agttaaaatc accacaccta ttaatcactt
     6541 ttttcgttga aagcaagatg tttcaacgct ctattattca tagcatcacc ccttttagtt
     6601 ctacccacca tgagcaacaa gttttctaag tagttaagta agggcaaata aagactatac
     6661 ttaactactt agtatataaa cgatctcttt tattaacgta ccgaaaccgc taattgagat
     6721 acgttatatg ctgtttcaaa tgatcctgca ttaatccaac ttgccccaaa cacaggatcg
     6781 acaacatggt aaaaaccgtc tttaaatcca tcaacggcta ctacatgtcg attccaaata
     6841 gcatcgcccc aaaacattga tttatgttga gcaggttcaa aggcataagt tacccatgct
     6901 actacaggat ttccatttac aatctcatct tttaatcctt gtaccgatga accagaaata
     6961 tctacaacat ttccgccatt agcaacaccc catgaaacta caggagccgg attcatacct
     7021 tgataagttc catccacatt gtgtctccat tcaccagaaa aaccattatt tggattatta
     7081 tcaggagata gtggcattga tttaacaaaa gtcattaaat cttgatttgt tgcaacacct
     7141 ttataatgta acgcttctag caaagatgtt ccttcgcaaa gcattgtgtt accttgacta
     7201 ataaatggaa cgcctagcac aacattagtt ggttgttcgg gtttacttgg tgaagtagaa
     7261 ctgtcagttt catcactcga acctgtttgt gattgatcac tagaagatgt actggttgat
     7321 tcatttgcat catttgatgt tgattgtgag ttatttgttg atgttccagt atcagcttcc
     7381 gtagtgctac tttcacttgt tccctgtgat gcttctttct cacttgtaga attatttgta
     7441 ttagatgaac ttgctccatc aacacttgaa gcacttgttt ctgtcttatc agaagtgttt
     7501 gctgttggtt ccgattcttt tgtagtctcg gattttttgt tctcttgctc cgtattttca
     7561 acaggatcag gtttatcctt ttgaacagtt tgactaacta cttcgccatc tgcttcctta
     7621 actgttaatg tatctttact gatgttgaaa gttcgtccaa ttgggatagt ccgttgttct
     7681 ccttttgcca aatcaacatt gttcaattcg gcaaactcag caacagtagt cttgtaacct
     7741 aagcttttaa gcttctctac agttgaccaa atagtatctc cttttttgaa cgcataactt
     7801 gattgtcctg ttgtaatttg gatttgatcc ggtgtgtttg cagtccattc atcagcatgt
     7861 gaggttactg gtactgataa ccccgcaagt acagctgatg caaataaaac cataccaagc
     7921 gaatttttct tcatattcat tccttctttt tgtttttatt ttctgtttgt gatcgcaatg
     7981 cttctattcc ttcttccgtt ataatatagg ttttgtcacc cactttcttg aacgaacctt
     8041 taggcaaacg atttggataa tagttaatta tgtttgtaac ataatttcta cctttaccca
     8101 aaatcaaact tgcttccctt gcggtataca agttgtattt gtctaggttc aatttctttt
     8161 tgtttgcgat ttagacctac ccctttcgca aaatttattg tataataata gtaccattgc
     8221 actgtcataa ttgcaatatt tttttggggt ttatcttcat ttaatagcct aaaaatgctt
     8281 gttagataag gaatatctaa ctaatcaaaa caaaaaagga agttaagaaa aatgaacaaa
     8341 aaaaggaaat tatcagtatt aattacactt atttctatat tgactattct tagcggtatc
     8401 ttgtcgctga aaatattcaa tcaaataaac acaagctcaa atgccaaaga tggaatagtc
     8461 gtagaaaata cctctaattc taaagaaact ggtactacta caaaaaaaga caaatctgct
     8521 gagatcacta cgaaaaatta tttaataaag actgtttatt ttgatgaacg aaaaacagca
     8581 attctcaaag atgaggaagg atcagaatta aagctaccta taacggtatc cgataagttc
     8641 aatggaacgt tctccttaca aaccgctgaa ggatcacctg acaatctcaa agttgggcaa
     8701 acaatcattc ttggtaaaaa cgacgaactg tattcatttg taaaataaaa aaatggagca
     8761 aaggctagtc aagcccttgc tccattttcc attcttcaag aggtgtagct atttcaacac
     8821 caaagtttaa atactgtgga tgctgtagat acatgatatc cattctattt tcgataaact
     8881 cttttaacgt tgagtagatg tatttttcta atgctatatc tgcccaacta ccataatcaa
     8941 gtttattcgt gatcttgacg tttatttcaa tttgagaaac atcaatattt tgaacatcag
     9001 gaaacttatt cagtcttagc tcttctctaa ccgcttctac tagcggttca tattgtccgc
     9061 ctagttcgct aattgttgaa actatttcat ctgaattcca acgatcttcc aatacctttg
     9121 ctacattttc tttaggcggt tgtctttcta taaattcttc aggtgtttca tcaaactcta
     9181 ctctaaagtt caatttattt tgattgttag catacatata atcatctctg tttgattcaa
     9241 aaaagttacg aattcctgtg tctgatttat cacttaactc cttatttgct aaagtagccc
     9301 aaaaatccca agttccttta ttttcagtat acaaagtaaa attaatgttt atatagtttg
     9361 cagtttcagg atacccttgc tctctaagag catctttaac aatcactgct aagtcagttt
     9421 caaaattctc cataaaaaat tcactaaatt cttttggatt ccacgtttct tctaataact
     9481 tttctaattt acgatctgcc attattattc tcccccactt tactttcaat cttatatcta
     9541 aaactgctta aaattaagag ctatacctta tcatagaagg tattttatgc tttatttaag
     9601 tctacaattc tatattacaa ttatcatcta gaccattttc ttatgtcaaa taaaccatat
     9661 atattgtctt taatagtttt atttgacttt ttaagaaagt atttactttt gacttttttt
     9721 acgttttaca acatatacgt attgtaatgt tcagctcaat ttattataat ctaaatgaat
     9781 tgattaataa aaattctgag gttcgggttt tttatcaatt cttttctgtt tgtgtttgtg
     9841 atgaaacaat cctttttcgg taggttttgt tatcatcgtc agcacaaaga gctaacgttg
     9901 gtaccgttag ctcttttttt attttactct gctcttacat agctatctaa atcctccaag
     9961 taaaacaatt gattttctcc tatgcttgtt actccacgcc aaattgttgt tgttgctgtt
    10021 gttggagtat ttcttgcctg tgtaatcaat tcaaccattt tacctttttg atttgttgta
    10081 tagtaatcag cagtatagct agatattgga ctattaccag tgcctaccat cgtgccagaa
    10141 atatcaacag gactagactt tactatctga ggaaattctt tttgtatttc cttcatattc
    10201 ttcgtatatt tttcccatga attccagttg taaaataaat taaagaattt cctactttgt
    10261 tccttgattt cagcattccc caattctttg tattgggctt cctgcatagc tttcactttc
    10321 tggttattct tgactacttc gatttgttta cttttaaggt cttccaattc tttgacatta
    10381 ttatcataag catttttctt ttgaaatagt accacacaac aaaggatcac aattatggaa
    10441 agtattccaa tcgccttttt agcagtagtg atttcatgat tttgcataaa aagcaccccc
    10501 agtcactttc ttatcattat ttagtgtgat ggttaatgtg gtatcataca ctttttttcc
    10561 attgtattga acttcaaaat cggtcaatac ttcatacaac ccactactag atgtcttaat
    10621 tccaaagttt gttagcttaa aagtaaaagg cttatagtac tcgatttttt ctttatcact
    10681 actcttatca gtaattggtg gtttacctgt gacaataaat agttttctca aaaattcttc
    10741 acctgattga tctgcaaact tattttgaac tgatccattc aacatttctt gaatatcatt
    10801 ttctaaatct ttccgtaatc catccacctc atttggttta acctctttaa agaatgtcag
    10861 cttatcttca atatgcacac tgtgtttttc agcttctatg tcctgattct gcttattgaa
    10921 gaataggatt ccaacaagca gtgctaataa cataccagaa agcaaagaaa tgataattgt
    10981 atctttcact ttactattca tctatatcgt ccttcattcc aatttatttt tcgatttcaa
    11041 atttagcttt tcctacaccg ctaaagaaat gcacattatc tgatccgtcc actccatcaa
    11101 tcaaagttac cgaaactccc cttgttggag cttgggttgg tggaacatta aagttagcac
    11161 atagaaatga cccatcttca aacaccgcta taactacacc agtatgccct gcatcgccaa
    11221 cagcccataa ccatccatcc gaagcactaa acccataacc tacgattggt ttgttcgtta
    11281 ttttcgctcc tgccgatttg tagctttccc atacatagcc accatttcca gtagtcggtt
    11341 gattcccatc gtataattgg ctcatataag cccacgtcaa ctctgtacac tgaccttgat
    11401 atattccgtt aaaaggtgaa gttgaccaat ctggttcttt gaattttggt agcgtgatcg
    11461 ctttcccata ttttttcaaa gcttcttcat aagtcatatt cgctattact tgaccttttt
    11521 taccgttaat ttttccttct ttacccattg atccagaacc agaattagca tcatctgtag
    11581 ttgcacaggc atcttctact gttcctgatg ctgagcttga tgaattattt gatccaccac
    11641 cggtacttgt tcctccggca attttattga ttttgcttac atctttagct ccgtccatgc
    11701 ctttgctttt catgtattcc caaatttctt ttgcatttcc accacgtaca gccactacag
    11761 ctgattcgga atctgctgaa atttcccaat tcttatgaaa cgctacggca tttgccacaa
    11821 catcatcaga agcgtttaaa gcataatctt ttaatatagc aacaaatcca ccatcgcctt
    11881 tgaacataaa atccaattgc gcttctgtcg tccaccaagc gccaccaaag tttttcttcg
    11941 cccagtttac taatgcagta tggcgacctg ctgtccattg accatagcca atacccatca
    12001 gaccatcagt gaattgtttg ataaagtcaa cttctctctg tccatcaccg taataacctt
    12061 ctaccgttcg aggatcatta cctgattctt gtttccagtt acctagaata cctgcgatag
    12121 tagcacctga tagcccatat tccccattca tgaatttgta aatatcatca atattttttg
    12181 ctacatcacc tgatacagca ccactccctg tttcatttcc atcactatct tttcctgatc
    12241 cactagaccc ttgcgagcct tcagtagaac agttgaactg tgcatcataa gtagctattc
    12301 catcagcatg tgtcacttgc ggatttatcg tgattaaact cgctgtaatc ccgattatct
    12361 gcatcactgc gataagcaac gtgccgaacg ttttcttcga ttttttcaat aactgcgccc
    12421 ccaaaagtgt acatttttat gttattatga tattataata acataaataa gtagaaagga
    12481 aaagctaatt aatgactgat aaaacaggta atctacctaa actagctcca aaaacatctc
    12541 aaacaaagag caaaaaagat gaaaaaatta aaaatgctgt aaagaaaaaa tcagtaaagg
    12601 aatcatcctc gttactactt gatattccaa tgagtacttt tgatgaaata cttgatgaca
    12661 atacggatga aacttctgtt aaaattgcat ctcacgttgg aatgaacagt caaaatgatt
    12721 ctttttacca cttgttaaac agcctaagag agttggaaga taaaaaaaat caggtaatcg
    12781 catcaaaagc tcctgacctc acaaccgatt tggatgctga aattatccaa gtcaaacaag
    12841 atgagcgatc agttaaatcg ctactgaaaa cctattttga tggttttgat aaagaactag
    12901 ctccatatag ccaagcaatc ctaactaaac caatacaagc tccacatatt tcaatttccg
    12961 aacaaaaaaa gagtgatgac taaatagtca ttactctttt ttcaaatttt gtttattaag
    13021 gattatttgc ttattggaat ttcttttctg caacaatcaa gttctttctt tttggtactt
    13081 ttattcaaat atttgttatg cagttcttaa ttatttaagt agcatatata ccccacaaat
    13141 tactatagta atatttatta ataattgaac aatagctgta aattttatca tatctattag
    13201 ttcatccttt tctaatctaa aatttaaggt agcggcatac ttaagaatat aact
//
LOCUS       DAZKKW010000071        13244 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.072, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000071 DAZKKW010000000
VERSION     DAZKKW010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13244
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.072"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            179..1048
                     /locus_tag="ACG8PO_002033"
     CDS             179..1048
                     /locus_tag="ACG8PO_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFQ0207335.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            1257..2282
                     /locus_tag="ACG8PO_002034"
     CDS             1257..2282
                     /locus_tag="ACG8PO_002034"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFQ0207336.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            2321..3271
                     /gene="manA"
                     /locus_tag="ACG8PO_002035"
     CDS             2321..3271
                     /gene="manA"
                     /locus_tag="ACG8PO_002035"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HFQ0207337.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIATEPGK"
     gene            3365..5068
                     /locus_tag="ACG8PO_002036"
     CDS             3365..5068
                     /locus_tag="ACG8PO_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFQ0207338.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKKSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            5070..7103
                     /locus_tag="ACG8PO_002037"
     CDS             5070..7103
                     /locus_tag="ACG8PO_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HFQ0207339.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            7114..7554
                     /locus_tag="ACG8PO_002038"
     CDS             7114..7554
                     /locus_tag="ACG8PO_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFQ0207340.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAKTIEEVQMIFEHGHI"
     gene            complement(7754..8881)
                     /locus_tag="ACG8PO_002039"
     CDS             complement(7754..8881)
                     /locus_tag="ACG8PO_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0207341.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLPLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(8884..9561)
                     /locus_tag="ACG8PO_002040"
     CDS             complement(8884..9561)
                     /locus_tag="ACG8PO_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFQ0207342.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            9825..11591
                     /locus_tag="ACG8PO_002041"
     CDS             9825..11591
                     /locus_tag="ACG8PO_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol-specific transporter subunit IIBC"
                     /protein_id="HFQ0207343.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEALATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            11606..12043
                     /locus_tag="ACG8PO_002042"
     CDS             11606..12043
                     /locus_tag="ACG8PO_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFQ0207344.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            12056..13207
                     /locus_tag="ACG8PO_002043"
     CDS             12056..13207
                     /locus_tag="ACG8PO_002043"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFQ0207345.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
BASE COUNT         4496 a         2302 c         2735 g         3711 t
ORIGIN      
        1 cttgatgcag aacagctttt tcacaacctc tttttataag agtttttact cattaattta
       61 caaaaaacaa gcaaaatgcg aattttctca catttttttt aacaaaatca aagttattgc
      121 ttgaaagaag aagaatccat cgttaaaata aagcttgacg tagaggggag caagaaatat
      181 gaaagatttt caattggggg ctgccaccta tttaaaaagt gtagccattc gggtgaaaga
      241 ccgagataaa atgattgatt tttacaagaa cgttgttggc tttgatttga aaagagaaga
      301 aaacgaatta gccattttag gaacagaaga agctggaagt gaaatgttgc tgctggaaga
      361 aagtcctaga gcgaatgatc acatgggggt gatcaaaaag ctgaaccgtt tttcattgat
      421 tatccctagc acagaagaaa tggcagatat cctttgtcgt atccgtaaaa tgaattatcc
      481 aatcaaagaa gctttagaag atcatggacg tatggggatt ttgctcgtag acccagaaga
      541 gaatgaattg gaaatttacc atgaagagaa taagaacgac caaagagaaa ttccagaaac
      601 aatggatcaa gaagcattat taagaaaatc tgaagaaaaa ttcagtaaac tatcaacagg
      661 ttcttatttt gaaaaaatcc atttaaatgt tactaactta acaaaagaac gccaatttct
      721 taatcatgta cttggtttga aggtacataa tgaagaagag ggcatccatg tattgaatga
      781 aggagatttc catgttggtt tgacagaagc tagcggaggc gcaattgatt tgcctacaga
      841 tgaagtgatt ggacttgatt ttctgaaatt catgatcgat actgaaagca tggaaggact
      901 gattgctcac ttgcagcaac tatcagttga tttcttctta gacaaaaagc acacagtact
      961 cacaatctat gatgctatag gaatcgaatg gtggttcgtc agtgagccaa aagaaaagaa
     1021 taaaactgac attcgatgcg acgcttaact ttttctttct taacgattgt cgcatgtctc
     1081 gtcattttat gaacggtcat actatcgctt attctagaga tagaaagaga aaatagtata
     1141 ccgggggaaa ctgcgcggtt tttcgcgcag tttttttgtg taaaaaaggt tgattcctca
     1201 tagattaagc tttaagcttg gactgtaaga atctgataga aaagcaggag aaaaagatgt
     1261 atccacacac gaaacagctt attttgaaaa agatggaaga aggattttat ccaggtgtag
     1321 cagtcgcctt cataaaagaa aatcaagtag aaacatttac agccggcttt gcccaagtcg
     1381 ttcctgaaaa gcagctgatg acagaagatc tgttgtttga tgcagcctca ctgacgaaag
     1441 tcgttgcaac cacttcatta gttttacgtc tattagaaga tggaaagata gaactcgatc
     1501 aaccgcttca taaatatctt cctgaatttg aaaatcagac aatcactttg cgtcatttac
     1561 tgacacatac ttctgatatc aaaacatgga taccaaacag agataagcta tctcaggaag
     1621 aattgatcgc cgcttatttg aagctagaac caggtgagca actaggaaaa gaagtaaaat
     1681 atacagatgc cggtacgatt cttcttgggt tgatgctaga ggaaatttac caagaaactg
     1741 cagtggaaat ctttcaagaa caagtattgg aaccattagg aatgatgaac agcttatttc
     1801 ttccttttcc ttccaaaaaa atcgttccca cagaacaact gccttcgggt gaggttttgc
     1861 gtggacaaac tcatgatccg aaagcaagag ttttggctga acatgcaggt aacgcgggtc
     1921 tctttattga tttgaaagac agcattactt ttgtccaaat gtatttgaac aagggaaaaa
     1981 tccgtaatgg tcgtttttta gaagaagaga cgattgattc tttactgcaa gatcaaacac
     2041 cgaaaaaaaa tggctatcgt tctttaggat gggacttaaa aaaggatgta gaagacggtc
     2101 atgcgattct ttttcatact ggttatacag gtacgttttt cttgattgat ataaaaacac
     2161 aagaagcgtt gatatttttg tcaaatcgcg tacatcctgt cgatcatcgt gcggagtatc
     2221 tcatatgtcg agatgaaatt gttgaaaact ttttgaaaga gaaagcggta ttaccgaaat
     2281 aatgctataa tagctatgaa aaatgagagg agtttgaatt atgcaggaac ctatgttttt
     2341 aaaaccagtt tttcaagaaa aaatttgggg aggaagtcgt ttacgttcag tctttggttt
     2401 tgatatccct aacgataaaa tcggagaaga ttgggcgatc agtgcgcatc ctcatggtgt
     2461 cagtgtagta gaaaatggcg aattcaaagg gaaaagatta gatgaactat ggtcagaaca
     2521 caaggaattg tttggccatc cttcagaacc agtgtttcca ttattgatca agatcttaga
     2581 cgcagaagat gatctatctg tacaagtaca tccagatgat gcctatggga tgaaacatga
     2641 aggtgaatta ggaaaaactg aatgctggta tatcatcgat gctgaacctg gtgcagaaat
     2701 catctatggt catcatgcaa agacaagaga agaactagct gagatgatca aagatgggcg
     2761 ctgggatgac cttttgaaga aagtaccagt taaaaaaggt gatttctttt atgtaccaag
     2821 cggaacgatc catgctattg gtaaaggaat catgatttta gaaacacagc aaagttctga
     2881 cacgacttac cgcgtgtatg actacgacag aaaagatgcg caaggacaaa caagagagct
     2941 gcatatccaa caatcgatcg atgtaacaac tgtccctgcg aaaacaccag aattacaaat
     3001 caaagaagtc cgaaaaggga attcatcgat cgtcacttat ttagaaactg agtttttcaa
     3061 tgtatatgag tgggatatta aaggaatcac atctttcaaa aaacaagccc cttatacatt
     3121 gatgacggta atcgaaggag caggagacct tgtcgttgac ggaaaaacat atccattaga
     3181 aaaaggaaca agctgtatta ttccaaatgg cgtttctgag tgggcggtcc aaggtgaact
     3241 agcaatcatt gcgactgaac caggcaaata gttcccagat agctgaaagc agcagacggt
     3301 ttttcgtttg ctgctttttc tacataaaat ccgtccatca ataaatagaa gtatggtgaa
     3361 aaagatggaa tacaaatgga aagcaaacaa cagcagtcgc gtgtctatga aacaactgag
     3421 aaaagctgga catcacttaa atgaaatgat catgccaaat ctaagtctgt ttatcgcttg
     3481 gggaatcttg tcattagcaa caaaaggtgt gtctggcgat ttgcacacca cattagaaga
     3541 ggtatgccat tggatgatcc agctgctttt acccatcttg atcagttatt caggcgggaa
     3601 atcggtaggg ggacaaaaag gggcagtcgc cggtgcaatt gcttctttag ggttgatcgc
     3661 tcgttccaat gctccacaaa tcgtaggagc gatgattatc gggccgcttg ccggcatcgt
     3721 atatgaacaa tttgtttcta gattccacgt gaaattcaag gctggatatg aaatgcttgt
     3781 cagcaaccta ctagtaggac tgactggtag cgttatttgt ataattggaa tcgtttttat
     3841 tgaaccagtt ttaaatagtg ttcacttgtt gctcgcctcc gttgtgtctt ggctagtcac
     3901 aatgaatctg ttaccacttg tcagtctgat cttagaacct ctaaaagtac tatttttcaa
     3961 taatgcaatc aatcatggtg tattgacacc tttaggaatc gaattctcac aaacagtcgg
     4021 gtactcttcc ttatttttga tggaggcaaa tcctggacct ggtttaggga tcttactttc
     4081 tatcctttgt tttgcagaaa aacaggaaaa agtaaatgcg agtggagcat tgatgatcca
     4141 tgcgattggc gggattcacg aaatctattt tccatttgtc ttgctgcgac cgtatctatt
     4201 tttagctgta atggctggtg gggcaagcgg cacgttgata tttcaatggt tagatacggg
     4261 attagctgca cctgtatcgc ctgggtcact actcgtgatt tttgccaatg cgccttatcc
     4321 acaactagct ggggtaagta tagggatagc aatcagtaca tttgtgacct tcatttgtgc
     4381 agcgatacta ttgaaattac caaataaaga aaaagagaac aaaacggtca aagaaagggg
     4441 agaaatcttg gaaccatcta tcattcattc cgtcattttt gcttgcgact caggtatggg
     4501 atcaagtgct atgggagccg ccctgctgaa aaagaaatta gcagcagaaa aaatcgatct
     4561 ttctgtcgga tacagctcta tctatgaatt gaataaccaa gtaaatcagc tggttatcgt
     4621 gcaacatgaa ctagcgggtc ttgcaagaga aaaagtacct caaagtcaaa tctatgtact
     4681 ggatcacttt ttggaaacag atatcttgct gtcacgaata aaagaagcga tccaatttcc
     4741 aaagcgggaa gaaaaaaaag agatagcaga tatcacagaa caaaaaaagt cgcctgctaa
     4801 tattgtattt ttgtataaaa acaacagacg cggctcacaa actatgggca tgacgatttt
     4861 aagtcaatta gcaaaaaaag cgaaaaaaca gtttgttatc tcaaaagaat caatagaaaa
     4921 aatcaaaatc caagaagaaa cgatttatat cgcacaaaga gaactagctg taactgaaca
     4981 gctagaagaa aagatccctc ggctagttct agttgaacat tgggtagcta cgaatgaata
     5041 cgaaaaactc gtgaagggag aaatataaga tgtatttatc gcccagagaa aaacaattat
     5101 tagcagaatt tctcagtagc ccgtcacctg tatctatcca aaaaatgatg aatttattga
     5161 aggtcagtaa acggactatt taccgcgaat tagataatct tgaagtctca ttgaaatcgg
     5221 taaatgcttc tttgaaaaaa gcagctagag gctcatttgt cattgaggca tcagaagaag
     5281 tattagagca attaaaagaa aatacagatg ggaaaagtaa tgacttatcc actgctaaac
     5341 gacaacacgc gattttagcc gagttgttac tgacgacaga gccgttgagt cttgctcatt
     5401 tcatggaatc atatcgcatc agtaatacca ctttttatgc agatatcaaa cagttggaag
     5461 aaagtattcg ccaactgcct ttggaaatca ttcggaacca gggatacgag atcgaaggac
     5521 cggaaaaata ccgccggctt ctgacagcga atgtattaga attagaaatc aatgaatatg
     5581 aactttttca tagtatttct ttcgatgcat cactgaatta tttttttcga ttcgttgatc
     5641 cgcaacatct atctcttgca cgcaaagtaa tcggggaaga attgatccaa aaaaagacca
     5701 atctaagtga tagaaagctt gaacatttgg ttttgatgct gacattgacg atggagcgtg
     5761 tgactaaaaa tcatttactg acaaatgaga catataatag tctggcaaac aaagaattac
     5821 tacagacagc taaacgattg ttttcacgga tcgcttctga gacaaaacaa ctttatccag
     5881 taaatgagat cgtctttttt gcaggattat taaatgactt cacgaactca tttgatcaga
     5941 atttctttga agagacgttc gatacacagc ttgcttattt agtaaaacaa ctgattcaag
     6001 aagtaagtga agagacgaat gtccattttt atgaagatga aactttgtac aaaatgctgt
     6061 tgactcattt atctggtgtg ttttcaagag ccgttttaca agaagagaca ctgaccaatc
     6121 cgattttaga aaaaatcatg gttcaatacg aggaagttgc gaatgcaatc cgtaaagcag
     6181 tagagcggat ttttcaagaa aaaaaattat ccgaagaaga gatcgcttat atggtccttc
     6241 attttgcgaa ttcattggaa cgaagtccga aagaaagcaa tatcagtatt gcaggatttt
     6301 cgccaagtgg attagcttct actagcatgt tggaactacg attgaaaagg tattttccct
     6361 tcatccaaca aattcatttc tatcggattg ctgatttaaa aaaaatcgat ttacaagaag
     6421 aatatgatgt tgttatttct acatctattt taccaggata cggtggaaaa tatattttag
     6481 tctccccgct actgctagaa gatgaagtca agcagctaaa agaagctttt tcgcatattg
     6541 agaagaaaaa aaggcaaaaa attacgtata acagcaataa cgagtggatg atagaagata
     6601 cttatgagga aacaatgcat ttcatggatc aaatcaatca attactcggt cgttttttta
     6661 tccaagaact ggataatcct gagacgattt ccgaaacgat tcgcagctta tttccttatt
     6721 ttcctgaagg actagtaacg gatgaagagg tggttcatcg ccggctgctt aagcgatatg
     6781 aacaagcacc tatcggtatt cctcatacaa atatggggtt attccataca tccagctcat
     6841 tgatttctaa accgatcttt tgtattttca atttgaaaca tccgttgacc attgaaggga
     6901 tggatagaca gccagtcgat ttgtcccgta tgttagttat gctggcgcct tcgccaatcc
     6961 aagaaaaaga cgggattctt ttaggaaaga tcagtggagc aatcatcatg aatgacttga
     7021 acaccgagat cttccgctca ggaaataaag aaatcgttta tcagttgtta gcgaaaatcc
     7081 tgattgaaga aataaagaaa tgaaggtgaa aagatgatac agctaccagc agaaaaaata
     7141 ttgctcgatc aatcctatca aaattggaaa gaagcgttga ccgagatcgg ccagtggatg
     7201 acccaacaag ggggaatcaa accggcatat atccaatcaa tgatcgaaag acaagaaaaa
     7261 gcgagtgtct atatcgggaa tttcgtagcc ttgccacatg ggactgacaa agatgaagaa
     7321 gtagtagaag aaggaatcta tttagtccaa gtgcctgatg gtgtggactt tggaacaaaa
     7381 gaggaaccaa aaatcgcgac actcttattt gtagtggctt taaaaaaaga acatcagtta
     7441 gcggtgctcc aagatttagc ctttttctgt tcagacatcg accaagtaat ggcgctcagt
     7501 gatgcgaaaa ccattgaaga agtccaaatg atttttgaac atggacatat ataaccaatg
     7561 ttcacagtaa aaaaagctgc cttgcatcaa gtaataactt caaccaagat aaaaataaga
     7621 ggagctattt ttgttgggag gttcttatta ctgcggatgc aagcttttga aaactattta
     7681 ttctagaaaa ccgtttattt gaaatagggg acggtgacaa ggtttttgtc accgccccta
     7741 ttatttttgt gttttaatag ctctttttta tatagatagt gcccatacct atgaataata
     7801 aaacgatact ccatatacca agtgtcaaga taccattcat gaaagtaacc tgttctgaag
     7861 ctagtggcga gaaaatatct cctcctatga tcacattttg aaaatcaaca taagaagttg
     7921 gcaagtaatg actgattgat tctatccatc caatcgaaga aaacaagcga tactctgcta
     7981 agaaaagaca aagaagtgta cctaaaagat tgactagaga atgttcaaat aaagcagata
     8041 gaagaaaact gattgtactt aaaaagagca cccacaacaa caggaacaga aagctctgga
     8101 aaagatactc atacatcgga aggattcgta tctcctgacc aatgatcgtt cctacaggat
     8161 aacggaagtc tcccaaacca tttttgatta ttactagtat agagacgatc aaaaatggca
     8221 tcacgagact tagcatagct gcaccataat aaatggtaaa acggatgcta tgtttcttta
     8281 ccatactatc cggtaataga ttgactagag agattacctg ctttcttttt tcataagaga
     8341 tgatggaaga aagaaacaag gcggttatac ataaaatcaa aaaactagaa atcattcctg
     8401 aaaaaatatt ttcgtagtaa tttgctaaag ggagatgcgc tggcaagtca aaaatagaat
     8461 atctttggat ctgatgttct ttcatataac ggagcaactc gacacgtttt tgttgttcaa
     8521 tgattggaat tccttgtctt tgaccagaaa tcagtcgatc cagtatatct ttttcataat
     8581 gataagttgc ttcgaccatt tgctggacat tccctgtgtt ttttgcatgt aagattgctt
     8641 cgatcagttt gttgcttttc tctaaacttt ccacaaattc tttctcattc tcatcttctt
     8701 tcaacgtttc aatcaaacgt tgcatctctt catagttatg attcagttca tcgaattctg
     8761 tataagcaag tttatgatca aaaaaaagtg ggaatgcaaa gagcacgacc aacaatagag
     8821 gaaataaaaa gtttactttg ttttttagta agagattgcg ttcaaatgta agtaaagaca
     8881 tagttacacc tcatataatt tacgataaat agctaaaatc tcttccatat ttgaggattc
     8941 tgtgacgatt tctccatttt ttagaaaaca tgctttagaa caaatcgatt ggatattcgc
     9001 gatagcgtgg gaagaaagaa gaatcgtctt cccctctttc tgccattgtc tgatgaggct
     9061 attcaagata tcaatacttc ctgggtctaa tccgttcaac ggttcatcga ataacatgac
     9121 agatgaatca ctgatcaagt aagcagcaag caagacatgt tgtttcattc ctagagacat
     9181 ttttccaatc ggcaaagtaa ggtactcagt catcttcaat cgttggaaaa cagtttggca
     9241 ttctactttg ctctgccaat agtatttgat caaagtcaaa taatcaacag ccgatagata
     9301 gtcggataac tgattcgttg attctagaaa aaagaaagac cggcgataat ctgtataagc
     9361 agtaggagag atgttgtgaa gggacaattg atcataagtt gttggcagca gttgggcaat
     9421 gccattcagc aaagttgttt tgccaaatcc gttcggggca atcaaagcag ctacttctcc
     9481 tgattctact aaaagattca attgtcgaat cacacgtttt ttaccacgtt tcatgttcat
     9541 gtttttgatc agtaatgtca tagataggcc tccaatataa aatttaataa cataatagaa
     9601 tggaaaacat ctaaattaaa tgctaacaaa agttattgtt tgcatattat aaaaaaatta
     9661 aaataacagt tttatttaaa attgaagttt tctgataccg aaatcttttt attttgctga
     9721 catactcaaa cctttggcac aaagtttcgt gccaaagtac gactatttct ctttttagta
     9781 agcggataca attagagtat caaaaggaga gagggagaga caaaatggaa cggacgattc
     9841 ccaaaaacaa catttcgcta aaagcgaggg tacaaaaatt aggcagtact ttatcaagca
     9901 tggtcatgcc aaatattgga gcactgattg cctggggtgt attaacggct ctatttatcc
     9961 cagatggtta tttaccaaat gaggctttgg ctaccatggt aagcccaatg ctaacttatc
    10021 tgattccttt attgatcggt tatactggcg gaaaagtaat tgctggtgac cgcggatcag
    10081 ttgttggggc aattgcaacg atgggtgtga ttgtcggaac agatattcca atgatgctag
    10141 gcgcgatgat catgggacct cttggtggat atgccatcaa gaaatttgat cagctattcc
    10201 aaaaacggat caaatccggt tttgaaatgc tagtcaataa tttttcagca ggtcttatcg
    10261 gttttggatt agcgttatta ggtttttctg cgattggtcc agttgtcgat gcattgactc
    10321 aagcaatggc aaaaggtgta gagatcattt tatctgccca tttgattcca ttgacaagta
    10381 tttttattga accagcaaaa atcttatttt tgaataatgc aatcaatcat gggattttga
    10441 caccattggg aactgaacaa gtagcagagg ctggaaaatc tattctattc ttgcttgaag
    10501 caaatcctgg tccaggactt ggcgtattac ttgcttttac tttgtttggc aaaggagcag
    10561 caaaatcttc agcaccagga gccatgatca ttcacttttt aggcgggatc catgaaatct
    10621 attttcctta cgttatgatg aaaccgttat tattcttatc agttataggc ggaggaatgt
    10681 caggaagttt cattttccaa ttgatggatg ctggactaag agcgccagct tctccagggt
    10741 cgatcattgc gattctagcg atgacaccgt tcaattctta ccttcctgtt attttgggcg
    10801 tgattgttgc cgcagctgtt tcgtttggta tttctgcagt aattttgaaa gcggatgcga
    10861 aagatacaaa tgaagacttc gaaaaatctg ttcaagagac aaaggcagct aagcaagctt
    10921 ccaaaggtgc ccaaggtatt cctcaaggag ctacattctc tggcgtacaa aaaatcattt
    10981 ttgcttgtga tgcaggaatg ggatcaagtg cgatgggcgc ttcgatcttg agaaaaaaag
    11041 tacaggaagc agacttgcct caaacggtga ccaatcaagc aatcagccaa ctgacagatg
    11101 actcgaatac attgatcgtt actcaggtcg aactacaaga aagagcaaaa caaaaagcac
    11161 cgagcgcgcg ttttgtctca gtagaaaact ttttgaattc acctcgatat gatgaaatca
    11221 ttcaagaatt aagcagcaag cagaaaacat cagtttctga aattgagaat gagccagcag
    11281 aagagtttct ttcagcaaac atcaaacaaa tcaaagagat tctacttgtc cacgatgatc
    11341 gttccggatc agcaacaatg gggatgaccg tgctcaaaga tatattagac aagaaccatt
    11401 taactatccc gttaagaaaa gtcaatatcc aagatttaaa agatgaacct gattcactga
    11461 tcgtagccaa agaaagttta gcggaaaaag tacagatggc tgcacctcaa tccattcatt
    11521 tatcagtcaa cagcatggtc aatccgcaaa aatacgaatc gattgcacaa tatttgaaac
    11581 agacagctta agggaggaaa acagcatgca aagtttgcca ttcgacatga tcgagctcaa
    11641 tcgatctttt aaaaccaaag aagaagcgat ccgctattgc ggtcagatgc ttgtagaagc
    11701 agggtgcgtt gatgaggaat atatcgatgc catggtagat agagacaaaa tgttatctgt
    11761 ttatatgggg aattttatcg cgattcctca tggaacagat gaagcaaaaa aatatgtgaa
    11821 aaaatccggc atctgtgtcg ttcaagtacc agatggtgta gattttggta ccgaagaaga
    11881 agaaaagatt gcgactgttt tgttcggtat tgcaggagtc ggagatgagc atttgcaact
    11941 tgtacagcaa atcgcacttt attgcagtga tatggacaat gttgtccagc tagcagacgc
    12001 gctcagcaaa gaagaagtaa cggaaaatct agcaattgct taaggagaga gaaaaatgag
    12061 agcagtacat tttggtgcag gaaatatcgg tcgaggattt attggagagg ttttggcagc
    12121 taatggattt gagattactt tcgtagatgt gaatcacacg atcattgatg ctttaaatga
    12181 aagaaaagaa tacgaaatcg aattagcaga tgaatcaaaa gaaaaaatca ctgtaaaaaa
    12241 tgtcaaagga atcaacaatc aaacaaatcc agaaaaagta atccaagaaa tcgctgaagc
    12301 agatttagtt actaccgcca ttggcccgaa tatcttacct tttattgctg agttgattgc
    12361 aaaaggtatc caagaaagag aaaaggaagg aagcacaaca tctttagata tcattgcctg
    12421 tgaaaatatg attggcggtt cagcatttct agaaaaagaa gtgtataaat atcttccgga
    12481 aacatcattt actgacaaat atatcggctt tccaaatgca gcagtcgatc gaatcgtccc
    12541 attacaacac catgaagatc ctttattcgt ccaagtggaa cctttcaaag aatgggtcat
    12601 tgacgatagc caaagaaaaa ataaaaaaat ccaattaaaa ggggttttat atgtagatga
    12661 tctagaacca tatatcgaac gcaaactatt tagtgtcaat actggtcatg cgactgtcgc
    12721 ttatacaggt gccttgttag gttaccagac cattgatgaa gcaatgcaag atgcgttagt
    12781 tgtggcacaa ttaaaatcag ttttgcagga aacaggaagt ttgctgattg ccaaatgggg
    12841 gtttgatgca gagcagcatc atgcgtatat tgaaaaaatc attcatcgct tccaaaacaa
    12901 aaatatctct gatgcgatca cacgtgtcgc tcggacacca ttacgtaagt taggttatca
    12961 agaaagattt acacgtccag tgagagaact acaagaacat aacttgactt gcccacattt
    13021 gacggcaaca atggggatta tttttaatta ctatgatcct gaagatgaac aaagccgtca
    13081 attgcatgaa atgaagatcc atgaaaactt ggaacaattg attcaagaag tcacaggaat
    13141 caatgatccg aaaacaatcg ggaatatcaa gcaaaacgtg aaccgttatg ccaagcaagt
    13201 cgcttaaaat aggatacgaa aaaggggaat gtgacgaaat tttt
//
LOCUS       DAZKKW010000072        13200 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.074, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000072 DAZKKW010000000
VERSION     DAZKKW010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13200
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.074"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..998)
                     /gene="uxuA"
                     /locus_tag="ACG8PO_002044"
     CDS             complement(<1..998)
                     /gene="uxuA"
                     /locus_tag="ACG8PO_002044"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFQ0207346.1"
                     /translation="MEMTFRWYGANEEKISLQEIRQIPAMKGIVGTLMDIPAGEVWPR
                     ERIKSLKDEVEANGLTLKVIESVNVHDDIKIGLPTRDQYIENYKQTIRNLAEFGIEVI
                     CYNFMPVFDWVKSDLDYRLDDGSSTLAYISKNIPTDPEEIIREVENSTNNFELPGWEP
                     HRLSQIKQLLEAYKEVDEEKLRENFAYFLKAIIPTCEEVGIKMAVHPDDPPYSIFGLP
                     RIVKNRDDLDWLCNVVDSPSNGITLCTGSIAERPDNDVYEILSEFCQRDRIPFAHVRN
                     IKYLSDNGLDFYEAPHKSEYGSLDMYKILKTMYDNGFNGYIRPDHGRMIWGETGRPG"
     gene            complement(1001..2632)
                     /locus_tag="ACG8PO_002045"
     CDS             complement(1001..2632)
                     /locus_tag="ACG8PO_002045"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFQ0207347.1"
                     /translation="MVSIKNWQNSIEEFNEMQILLPCFDVKEVKQEGQQHPVWLHFGG
                     GNLYRGFHAEIAQCLADQNELETGVVVCETFDEEVVEKAYHDYNNDILEIVMSGDGEL
                     KKRLLAVTADSLYCNPKNIADYQRVVKYFEDPKLQFTTFTITEKGYSLKDSKGQFFSV
                     VKADIKSGPSNPQHTMSIVTSLLYSRFNAGELPIAMVSTDNFSQNGQRFQDSIFTIAE
                     AWQKEGYVSEKFIAYLKDKEKVTFPWSMIDRITPNPSENVKEDLLMMGLEDLDIIHTK
                     KHTNIAAFTNTEVVHYLVIEDSFPNGRPKLEKAGVIMTDRETVDKADIMKVTTSLNPL
                     HTALAVTGCLLGYQSISAEMQDDDLVGLIKGIGYLEGLPVVENPGIIDPKQFIDEVIN
                     KRLPNPYIPDTPQRIATDTSQKVAIRYGETIKKYVEKDGNAENLTFIPIAIAAWLRYL
                     LAVDDQGENFVPSPDPLLVELQDKLSVLKLGEQDPSKIHCAVQEILENKEIFGLDLYQ
                     VGVGKKTEKIFCEMLVGPQAVRKTINKYITEVGGK"
     gene            2936..3607
                     /locus_tag="ACG8PO_002046"
     CDS             2936..3607
                     /locus_tag="ACG8PO_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0207348.1"
                     /translation="MTIKTSLQNQAYQSIRRQIIYADLEPGKKVSEKKLEETLSIGRT
                     PIREALIQLRQQGLVDTIPQSGNYISLIDLDSAKNARFVREHLERQIMLECCAKMNNQ
                     KKRILETIISEQEKAVSQKNARSFFHNDNLFHKACFEIAEREEVWQWLENNNTHLERF
                     RWLRVKTEGLKWEKIMEQHYQLFEALTKQDPEEANFLTSLHLHLMLNEQNIVLATHPK
                     YFKNI"
     gene            complement(3767..3958)
                     /locus_tag="ACG8PO_002047"
                     /pseudo
     CDS             complement(3767..3958)
                     /locus_tag="ACG8PO_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
     gene            complement(4326..4667)
                     /locus_tag="ACG8PO_002048"
     CDS             complement(4326..4667)
                     /locus_tag="ACG8PO_002048"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0207349.1"
                     /translation="MSIDDCLEEQIRLFLKFLKVKVQNNYFTCYSYAHPVLNSLPFNS
                     LVQGLITNIVLLVCTPNEIFVKKIGSGISSVRLKEKEYTQKIIHIPKPEIKRFEIKDY
                     SALGLFSIGYW"
     gene            complement(4764..5216)
                     /locus_tag="ACG8PO_002049"
     CDS             complement(4764..5216)
                     /locus_tag="ACG8PO_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0207350.1"
                     /translation="MKSLEQEHMAFKQAMFKENIYLNHNYIRVSKACSPVLNMLGGGN
                     GLYHLLFVDVCWLIFLPDELVIVNEKITSKNEVFNYSLTRINYKEITKFSLEKVPFWG
                     EFCLKIKCNWKRMYFYIDGDDALTFGKTTFSSFNFQFLLKNNFYGLLK"
     gene            complement(5223..5771)
                     /locus_tag="ACG8PO_002050"
     CDS             complement(5223..5771)
                     /locus_tag="ACG8PO_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HFQ0207351.1"
                     /translation="MKRIIKIFGVLGVFLLGLSTFVSTGTSVYATEKVNTVDLEIKNK
                     LVDELTFYFDTVGFLDEHGVYHIKDFGAIRERISQDNEQGRAARVFYEHIIVPRMEKV
                     DWKRYAGCVAINSLPGGGVAWAIGTQGDFIEALKAFNFEKAADLLLKIAEKQLGKAAA
                     KELAKVNIVAGIAAAAASCALE"
     gene            complement(5816..6148)
                     /locus_tag="ACG8PO_002051"
     CDS             complement(5816..6148)
                     /locus_tag="ACG8PO_002051"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0207352.1"
                     /translation="MSEEKKEIVESLVALKDSLSKIEYVDRQEIQKLIDDTIIEIQDA
                     RCEGIKISVALSKVIEKMNRSLAFNGLKLDRQTSLIWDHLKDLYDKSKRSERTAVSIL
                     KGLWGMNS"
     gene            complement(6162..6419)
                     /locus_tag="ACG8PO_002052"
     CDS             complement(6162..6419)
                     /locus_tag="ACG8PO_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003653123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0207353.1"
                     /translation="MKKLIGYSVMCIGFVGVGLFVSTESASAAEWYNPIWYNPNIECQ
                     KGYPYGPTNLKNGKCRVNWNGVVDSVITGSMDSALHGFTGH"
     gene            complement(6614..7156)
                     /locus_tag="ACG8PO_002053"
     CDS             complement(6614..7156)
                     /locus_tag="ACG8PO_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF0163 family protein"
                     /protein_id="HFQ0207354.1"
                     /translation="MKKGIILVFSWLCLIGLTGCHWFSSEKQKTQLRTTEETIEAVKE
                     TSGTLQSTESNSQIGSLEDETAQQMQIDEMTKNFLKDFARKWSNFTDVYKRNQAVRDY
                     MTDRCIKDNSIDVDPHVQMKTTGKLYQVTQDIENPDQYLLFGEETMNKVTQFVLLQLK
                     VDQEAQKIDAIKIYYVRSAY"
     gene            complement(7177..8268)
                     /locus_tag="ACG8PO_002054"
     CDS             complement(7177..8268)
                     /locus_tag="ACG8PO_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFQ0207355.1"
                     /translation="MKKGCLGCLLFFILPFFLLPFFFSGELSEHGGEATGLAVAFNGE
                     YTEGLPIFSEIKGRGPITDEHAQAAVGAAVKYQLLPSVILSQLGYESAYGQSQSGRND
                     TNFFGITWFPGCPYPQGSARGIGGSEGGFYMKFPNLKACYDYYGFMVASQSNFNQCVG
                     NKDPGSCLLILGRGGYAAAGITEGSPYYRGAMSIITSYNLTEYDTFAIKHWNSLPTKK
                     TSLIAGKSKGNAKVLDRIIGRSVNNGECYGGTAWYVSQLGGPQLMGSGHMFAMLIGED
                     YNWTTYGWQVIMNPKPSDLQAGDVVNWQAGGALSPGIYGHTGIITDVSYGGQHFSSVE
                     QNAEQGRIMARYQRTYNQTKIRSLVRKVK"
     gene            complement(8276..10486)
                     /locus_tag="ACG8PO_002055"
     CDS             complement(8276..10486)
                     /locus_tag="ACG8PO_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFQ0207356.1"
                     /translation="MAYIKHKEKKWCVLFFLTIGFGLLFVGGLVYAAEKQVDQSIYEI
                     GKFHIDTFSSYLSDSEFSLGKMLFGANGTDFLKMMTNMFFSLSKLIWQGLDFVIEKLY
                     EGAAMNELIHQFFTFSDTIYSKIYDPIGAGIVVLYVLYLFTLRIFRGKHYAKMVFIRF
                     LFVVIFSMVWFGYGSNVPSKGEAFTKGLNDLSVEVEGFIFSATNSLDELATKDVNGVL
                     VPTTQKEAIQKVRQLYYKAAVVDPYILLNYGTTNLEELYQAEIEPQEFLAKEASSEEL
                     SDIETKVTTAAGEDRGKKKAYRVYIQPTKAVYKAVVGSMVPLLNLSIGLPLVVIGMIR
                     FIFQMLVLCLLLVIPFSLLASFVPVFDYMLMNTLKSFLGLMFQKSFYSVIILVAFLIY
                     NIVDDLIPMNTVFGFLGNMFVKALLSILLIVKRKDLLKKLGLGAADQTINNTKNIASH
                     SLSQMKQLPGQLSTAGQNLAIKGAGLAGNVYRATNPLASAAIHGFQKFQQKRTPQESV
                     KGRQVVNPFELGGRSNQALPYSGRHVRYHGVEPEMSSTKSKTEWNSGLSNGLIPNSSM
                     NSVRPKQQVQSLAQLSKQQGNNQRKPLLNKGIAFVGQSIPLPSGKVPVPQLTAAGSLS
                     QQIRQVKNQAKNGSYPAMQQLLNQYSVPNSSVATLSGNRSVQQPGQRSSESKPSISAP
                     KSLQPAFSSLDKPTEQSEKIEKDPKTLPILDAKPGTDFTLPKGRTVQKPLSKKG"
     gene            complement(10502..13030)
                     /locus_tag="ACG8PO_002056"
     CDS             complement(10502..13030)
                     /locus_tag="ACG8PO_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFQ0207357.1"
                     /translation="MVLVKEPVKAFKKNCLLNGEDCIWAYYHVFPRTISKNTPKKTQE
                     IKEMMARLLLLLADYEDVELVMYPRTMNLRTKFDTLGKGFSPDSQAIGAYYAKETVTL
                     LEREGQVYRPSFILGIKVKEKATVESFHDVINLIWLDLAGSLARLFGLEVDDLETLFS
                     ESDPYVKEVTQLLYSFSIRPLVKKELLYLNRLQYIRGMAHKVEEQNNWEVLKDNQGTY
                     VRIPKEHKGLLQLEDELGSSYLALLPIGETPENMRNCDLFQFAQDLSFPAELRMKIHY
                     PRLKGQGGIGLKLNWLSERFKTEHKELVQNDEEPSERLLTVRGLVKHLKQRVDNGQPV
                     VDWIACFIVYGQTVEEVRQNSATVIQQLHTIIHIHRAKNDQRELFYKCLLGQPLGRQL
                     HWKHRTTVEAVGEWLFSAVSELGMESGWYIGRQDTLGDGGSRGEKTPLEELIYASNRF
                     VFLNLLAIAEGIKGALFDAPHVAVTGKTGKGKTFLVGLLFLYSSFMNVQSLFVDPKGE
                     KRKWFTKLIQDPYYQKHYPLFVEHLQKMSYVTLDATNPRNYGVLDPIVCLSRVDAKQV
                     AQDMIDELSPLGTNHLLKGAVLKGIQEVLVQKEAGQQVGLMHVVDYLEQMEQKEVREY
                     GEFLRLTVEDSILRLGFSYGENPGLDFNAKTTILEIQDLKLPNDNVRPELYTDADRKS
                     LCLMISLGRFCEMFGKRDSSKKTAIYFTEAWVFNNSNAGRSIIAAMARVGRSQMNQLV
                     LDTQFIGDLGSDKEKGNFGVVFAFDEDSEREKILNHLRLEVNEANLAEMSHMIKGQCW
                     MLDPYGRVGKLNVHSLFEEMTAALQTTEKTSHSQVEEQDTLIMQ"
BASE COUNT         4160 a         2718 c         1835 g         4487 t
ORIGIN      
        1 ccaggtctac cggtttctcc ccaaatcatc cgaccatgat ctggacgaat atagccatta
       61 aatccattat cgtacattgt ttttaatatc ttatacatat ctagagaacc atattctgat
      121 ttatgcggcg cttcgtaaaa atcgagtcca ttgtcactta aatatttgat attacgaaca
      181 tgtgcaaaag gaatccgatc ccgttgacaa aattcagata gaatttcata gacatcatta
      241 tctggtcgtt ctgcaatact tccagtacat aatgtaatac cattagaagg agaatccact
      301 acattacaca accaatctaa atcgtcacga tttttaacaa tacgaggtaa tccgaaaatg
      361 gaatatggtg gatcatctgg gtgaactgcc attttaatcc caacttcttc acatgtcggg
      421 ataattgctt tgagaaaata tgcaaagttc tctcgtaatt tctcttcatc aacttcttta
      481 taggcttcta ataattgctt aatttgtgat aaccgatgag gttcccaacc cggcaattca
      541 aaattattag tagaattttc tacttctcta ataatttcct ccggatcagt agggatattt
      601 tttgaaatat aggctaacgt agaagaccca tcatctaaac gataatccaa atccgatttt
      661 acccaatcaa atactggcat aaaattataa cagataacct caatcccaaa ttctgccaaa
      721 ttgcgaatcg tctgtttata attctcaatg tattgatctc gtgttggtaa accaattttg
      781 atatcatcat gaacattaac actctcaata actttcaagg taagtccatt ggcttcgact
      841 tcatctttta aagatttaat tcgttctctc ggccaaacct ctccggctgg tatatccatt
      901 aatgttccaa caattccttt cattgctggg atctgacgaa tttcttgtag tgagattttt
      961 tcttcatttg ccccatacca tctaaaagtc atttccatag ttactttcct ccaacttctg
     1021 ttatatattt attgattgtt ttacgaactg cttgaggacc tactagcatt tcgcaaaaga
     1081 ttttttcagt tttctttcct accccaactt gatataaatc taaaccaaag atttctttat
     1141 tttctaaaat ttcctgtacc gcacaatgaa tctttgaagg atcttgttca cctaatttca
     1201 atactgataa tttatcctgc aactctacca ataacggatc aggacttggc acaaaatttt
     1261 ccccttgatc atcgactgcc agtaaataac gtaaccaagc agcaatagcg ataggaataa
     1321 atgttagatt ttctgcattt ccgtcttttt cgacatactt tttaattgtt tcaccatagc
     1381 gaatcgctac tttttgagaa gtatctgttg caattcgttg tggtgtatct ggaatatagg
     1441 gattaggcaa acgtttatta attacttcat caataaattg ttttggatca ataatgcctg
     1501 gattttcaac tactggtaaa ccttctaaat aacctattcc tttgattaaa ccaaccaaat
     1561 catcatcctg catctctgct gaaattgatt gatatcctag cagacaacct gttactgcca
     1621 aagcagtatg aagtggattc aagcttgtcg taaccttcat gatgtctgct ttatcaaccg
     1681 tctctctatc tgtcataatc acaccagcct tttccaattt aggccggcca tttggaaaac
     1741 tatcttcaat aactaaataa tgaactacct cagtattagt gaaagcagca atattggtat
     1801 gtttttttgt gtgaatgata tctaaatcct cgagccccat catcaataga tcttccttta
     1861 cattctctga aggatttggc gtaattcgat caatcatact ccaaggaaaa gtcacttttt
     1921 ctttatcctt taaataagca ataaattttt cagaaacata tccttctttt tgccaagctt
     1981 ctgcaatcgt aaaaatacta tcttgaaaac gttgaccatt ctgtgagaaa ttatcagtag
     2041 aaaccatcgc aatcggcaac tctccagcat taaatctgct atataacaag gatgttacaa
     2101 tactcatcgt atgttgagga ttgctaggtc cagatttgat atcagctttg actaccgaaa
     2161 agaattgccc tttagaatct tttaaactgt aacctttttc agtaatagta aacgttgtaa
     2221 actgtaattt aggatcttca aaatatttaa ccactctttg atagtccgct atattcttcg
     2281 gattgcaata taaactatca gccgttactg ccaaaagtct ttttttgagt tcaccatctc
     2341 cactcataac aatttccaaa atgtcattgt tatagtcatg ataagctttt tccactactt
     2401 cttcatcaaa tgtttcacac acaactactc ctgtttctaa ttcattttga tctgctaagc
     2461 attgagcaat ttctgcatga aatcctcgat aaagatttcc tccaccaaaa tgaagccata
     2521 ctggatgttg ctggccttct tgcttaactt ctttgacatc aaaacacggc agtagaatct
     2581 gcatttcatt gaattcttcg atagaatttt gccaattttt aatcgaaacc ataattaacc
     2641 tcctcaaact gatatgttag ttttgataca ttttatattt taaaacccat tgtaaccgtt
     2701 gtcaatataa aaagggaata aacttgcttt tttctaccat atcagaatga ttgtattttt
     2761 tattcagaaa aataaaatac tccttctttt tggactgatt ttatcgaaac ttattttttc
     2821 tttttttcat gtattctttt atttttataa ctagtgtatt acttcaaaat ttgatattat
     2881 atggcatgaa cagaattaaa atatcagtta aaataaaagt taaggagcat tgtgtatgac
     2941 aattaaaact agtctccaaa atcaagctta ccaatctatt cgccgacaaa ttatctatgc
     3001 tgacttagaa cctggaaaaa aagtatcaga aaaaaaatta gaagaaacac tttctattgg
     3061 tcgtacacca atacgtgaag cactgattca actccgacaa caaggtcttg ttgatacaat
     3121 tcctcaaagc ggaaattata tctcattaat tgatttagat tcggccaaaa atgctcgatt
     3181 tgtcagagaa catttggaac gacaaattat gttagagtgt tgtgccaaaa tgaataacca
     3241 aaaaaaacgg attttagaaa ctataatttc agaacaagaa aaagctgtta gtcaaaaaaa
     3301 tgctcgtagt ttttttcata atgataattt attccataaa gcttgttttg aaattgcaga
     3361 gcgagaagaa gtatggcaat ggttagaaaa taacaataca cacttagaac gatttcgatg
     3421 gctacgagta aaaacggaag gattaaagtg ggaaaaaatt atggaacaac attatcaact
     3481 atttgaggca ttaacaaaac aagatccaga agaagctaac tttctaacat cgttgcatct
     3541 tcatttaatg cttaatgagc aaaatattgt tttagctact catccaaaat atttcaaaaa
     3601 tatataactt ttaactcttt ttaaattaaa tggttgttct gatcagaaaa atatcgcttg
     3661 ttttcttatt atgtaatata tttcataaac tattctgcat aacaactatt gtttgtactt
     3721 tttacattgg ataactaatt atgactgctg ataacttttg atattaaact agtatctcca
     3781 atacaaataa aaaatagata tcttgttcat caaaattagt tcgtggatac ttaccttcat
     3841 ttacatcatt cagcggtttc ccattttctt ttattctatt atttcatttt ctaatagact
     3901 tggcttcatt tcgcaatact ttttttcaat aggcatcaaa ttggtgttgt ttagatattt
     3961 agttgtagtt ttgtgacatg ataagtatgc tcctttttct cttttccact aatacaacga
     4021 tcaaagggtg cttttttcaa aaaactagaa aaatcaaaat aagattcgta ttttgattct
     4081 atatatcctg tttcaactac atcttatatt tcacaataga ataaatagcc ttttgcatag
     4141 ctgcttaact tataagaaat tgcaatttac ttcttcaaag tgaaaacgaa tatgagtaaa
     4201 ataaaattat tcttcttaca gataattaaa aatagcgcgt aacaaaaatc ggcattcaat
     4261 cacataatta ggattgatgc cgattactta tattctacgt atagttttag tattcttatt
     4321 ttttatcacc agtaaccaat tgagaacagc cctaaagcag aatagtcttt tatctcaaaa
     4381 cgcttaattt caggcttagg aatatgaata atcttttgag tatattcttt ctcttttaat
     4441 cttactgaag aaataccact gcctatcttt tttacaaata tttcatttgg tgtacagact
     4501 aataaaacaa tatttgtaat caatccttgc actaaagaat taaaaggaag actatttaaa
     4561 acagggtgtg cataactata acatgtgaag tagttatttt gaactttaac ttttaaaaac
     4621 tttaaaaata atcttatttg ttcttctaaa caatcatcta tactcatata atcactccac
     4681 taattaatct ccccaaaaag acacagcatt aagagatata ccaacacaac agtgcaaata
     4741 actctacatc ttccttcgca accttatttc aataaaccat aaaaattatt ttttaataga
     4801 aactgaaaat taaaactaga aaaagtagtt ttaccgaatg taagagcatc atcaccatca
     4861 atatagaaat acattctttt ccagttacat ttaattttta aacaaaattc tccccaaaaa
     4921 ggaacttttt ctagtgaaaa cttggtaatc tctttgtaat taattcttgt taaagaataa
     4981 ttaaacactt catttttact tgttattttt tcattgacta taactagttc atctggtaaa
     5041 aaaattaacc aacaaacatc gacaaacagt aaatgatata atccatttcc acctccaagc
     5101 atattcaata ctggtgaaca tgccttagaa acacgaatat agttatggtt taaatagata
     5161 ttttctttaa acattgcttg tttaaaagcc atatgctctt gttctaaact tttcataaac
     5221 ttttactcta atgcacagct agcagcagct gctgcaattc ctgctacaat atttacctta
     5281 gctaactctt tagctgcagc ttttcctaat tgtttttctg caatttttaa taataaatcc
     5341 gcagcttttt caaaattaaa agcttttaat gcttcaataa aatctccctg cgtacctata
     5401 gcccaagcca ctcctcctcc aggaagagaa ttaatggcta cacatccagc atatcgtttc
     5461 caatcaactt tctccattct aggaacaata atatgttcat aaaagactct tgctgctctt
     5521 ccttgttcgt tatcttgact gattcgttca cgaatagcac caaaatcctt tatatggtat
     5581 acaccatgtt cgtctaaaaa accaactgta tcaaaataaa aagttaattc atctactaat
     5641 ttattcttta tttctaaatc aactgtattc actttttctg tggcatacac acttgttcca
     5701 gtactaacaa aagtacttaa acctaataag aaaacaccta aaacaccaaa tattttaatt
     5761 attcttttca taatttctcc tccataaaaa ataagttact ttttaaatat cttgtttatg
     5821 aattcattcc ccaaaggcct tttaagattg atacagcagt ccgttcactt cttttagact
     5881 tgtcatacaa gtcttttaaa tgatcccata tcagactagt ttgtctatct agcttcaatc
     5941 cattaaacgc taatgaacgg ttcatttttt ctattacttt agataaagct acagaaattt
     6001 ttataccttc acaacgcgca tcttggattt caataatagt atcatcaatt agtttttgaa
     6061 tttcttgcct atcaacatat tcaatcttag aaagagaatc ttttaaagct actaatgatt
     6121 caacgatttc ttttttctct tcagacattt tcttcctcct tctagtgacc cgtaaagcca
     6181 tgtagagcag agtccattga accagtaatt acactatcta caacgccatt ccaatttact
     6241 ctacattttc catttttcag atttgtcggt ccatacggat agcctttttg acattcaatg
     6301 ttcggattat accagatagg attataccat tcagccgcag aagcagattc agtcgataca
     6361 aacaatccca caccaacaaa tccaatacac atcacactat agccaattag ttttttcatc
     6421 tcgtatccat ctcctaaaaa atagaatagt tcttatagtt attcataaga catatttttt
     6481 ccgtttttgt tttatccttt ctgtcctata acaacttttt tcaagtaaag aatgacatag
     6541 tttttcttaa aaaaacagtc tgtttttttc tatttcttcg gtgtataata aatatgagtg
     6601 ttcatttttt attttaatac gcactccgta cataataaat cttgatcgca tcaatctttt
     6661 gagcttcttg atctactttc aattgaagta atacaaattg agtaacctta ttcattgttt
     6721 cttctccaaa taacaaatat tgatccggat tctctatatc ttgtgtcacc tggtaaagtt
     6781 tcccagttgt tttcatttgt acatgcgggt ctacatcaat actattgtct ttaatacaac
     6841 gatctgtcat atagtccctc acagcttgat tgcgtttata aacatctgtg aaattactcc
     6901 attttctagc aaaatctttt aagaaatttt ttgtcatttc atctatttgc atttgttgcg
     6961 ctgtctcgtc ttctaatgaa ccaatttgac tgttactttc agtactttga agagttccac
     7021 ttgtttcttt tactgcttcg attgtttctt ctgtcgttct caattgtgtt ttttgttttt
     7081 cagaagaaaa ccaatgacaa ccagtaagac ctatcaaaca taaccaacta aatactaaaa
     7141 ttatcccttt tttcacgttt ctagtcactg cctttcttat ttcaccttac gaaccagact
     7201 acgaattttt gtttgattgt acgtgcgttg ataccgagcc ataatccgtc cttgctcggc
     7261 attctgttcc acactagaga aatgttgtcc gccatatgaa acatctgtta taattccggt
     7321 gtgaccatag attccaggag acaatgcacc acctgcttgc caattcacca catctcctgc
     7381 ttgtaaatca cttggttttg ggttcatgat tacttgccaa ccataggttg tccagttata
     7441 atcttcccct attaacatcg caaacatgtg tccagaaccc attaactgtg ggccacctaa
     7501 ttgagataca taccatgccg taccaccata acattcccca ttatttacgg atcgtccaat
     7561 gatccgatcc aaaaccttcg cattgccttt actttttcct gcaataagcg aagttttttt
     7621 tgttggtagg ctattccaat gcttgatcgc aaaagtatca tattccgtta agttatagga
     7681 cgttatgata ctcattgctc ctcgataata cggactccct tcggtaatac ctgctgccgc
     7741 atagcctcca cgacctagaa tcaataaaca acttccagga tctttattgc ccacacattg
     7801 attaaagttg ctttggctcg ccaccataaa gccataatag tcataacaag cttttaagtt
     7861 cggaaacttc atatagaaac caccttcaga accaccaatt ccacgtgcag aaccttgtgg
     7921 gtagggacaa ccaggaaacc aggtaatccc aaaaaagttc gtatcgtttc ttccagattg
     7981 tgactgtcca taagctgatt catagcctaa ttgagaaaga atcacactcg gtaataactg
     8041 atactttaca gctgcaccaa ctgctgcttg cgcatgttca tctgtgattg gaccacgtcc
     8101 tttaatctct gaaaaaatcg gtaatccttc ggtatattcc ccattaaaag cgacagctag
     8161 acctgtagct tctcctccat gctcagaaag ttctcctgaa aaaaagaatg gcaatagaaa
     8221 aaagggaaga ataaagaaaa gaagacaacc tagacaccct ttcttcattt cttcctcatc
     8281 ctttcttcga taatggtttt tggactgttc gtccttttgg taaagtaaaa tcagttcctg
     8341 gttttgcgtc caaaatcggc agtgttttag gatccttttc tattttttcc gattgctctg
     8401 ttggcttatc taaagaagaa aatgcaggtt gtaacgattt tggtgcggaa atcgacggtt
     8461 tactttcaga cgaacgttgc cccggttgtt gcacagaacg atttcctgat agagtggcta
     8521 ccgacgaatt aggaacagag tattggttca ataactgctg cattgctgga taactcccat
     8581 ttttcgcctg atttttcact tggcgaatct gttgtgaaag agaaccagca gctgttaatt
     8641 gtggcacggg tacttttcca gaaggcaacg gaatcgattg accaacaaaa gcgatacctt
     8701 tatttaacaa tggttttctt tgattattcc cttgttgctt actcaattgt gccaatgatt
     8761 gtacctgttg ttttggtctt actgaattca tggacgaatt tggaatcaaa ccatttgaaa
     8821 gaccagaatt ccactctgtt ttcgatttgg tagaagacat ttcaggctct accccatgat
     8881 aacgaacatg acgaccacta taaggcaagg cttgatttga acgcccacct agttcaaagg
     8941 gattaaccac ttgcctccct tttaccgatt cttgtggtgt acgtttttgt tgaaactttt
     9001 gaaatccatg tatcgctgca gaagcgagtg gattcgttgc tcgatacaca ttccctgcta
     9061 atcctgcacc tttgattgcc aaattttgtc cagcagttga tagttgacca ggcagttgtt
     9121 tcatttgaga caaagaatga cttgctatgt ttttcgtgtt gtttatagtt tgatctgcag
     9181 ctcctaaccc tagtttcttt aataaatctt tccgttttac aatgagtaaa attgatagga
     9241 gtgctttgac aaacatattt cctaaaaaac caaaaacagt attcattgga attaaatcgt
     9301 ctactatgtt atagatcaaa aatgccacca aaataatcac actgtaaaaa gatttttgaa
     9361 acatcaagcc caaaaaggac tttaaggtat tcatgagcat ataatcaaat actggaacaa
     9421 aagaagcgag taatgaaaaa ggaatcacaa gtaataaaca caacaccaac atttgaaaga
     9481 taaaacgaat catgccaatc actacaagtg gtaatccaat acttagatta agcaatggca
     9541 ccatagaacc aacgactgct ttataaacag ctttagttgg ttgaatatag acacgataag
     9601 ccttcttttt ccctctatct tctcctgcag cagtggtcac ttttgtctca atatcagata
     9661 attcttcact tgacgcttcc ttggctagaa attcttgtgg ttcaatctct gcttgataca
     9721 attcttctaa atttgttgtc ccataattta acaatatata cggatcaaca acagccgctt
     9781 tataataaag ttggcgcact ttttgaatgg cttccttttg tgtcgttggc actaaaacac
     9841 cattgacatc ttttgtcgcc aattcatcta gactatttgt agcagaaaaa ataaagcctt
     9901 ctacctccac ggacaaatca tttaaccctt tggtaaaagc ttctccttta ctaggtacgt
     9961 tactaccata gccaaaccaa accattgaaa aaatgacaac gaagagaaat cgaataaaca
    10021 ccatttttgc gtaatgtttc cctctaaata tacgaagagt aaatagatat aacacataca
    10081 agacgacaat ccccgcacca ataggatcgt atatttttga ataaatagta tccgaaaaag
    10141 tgaaaaactg gtgaattaac tcgttcattg cagctccttc atagagcttc tcaatgacaa
    10201 aatcaaggcc ttgccaaatc aatttactta aactaaagaa catattcgtc atcattttta
    10261 gaaaatctgt gccatttgcc ccaaataaca ttttgcctaa tgaaaattca ctatcagata
    10321 aataagaaga aaaggtatca atatgaaatt tcccaatctc atagatcgat tgatccacct
    10381 gcttttctgc cgcatacacc aatccaccaa caaaaagcag tccgaatcca atcgttaaaa
    10441 aaaacaaaac gcaccatttc ttttccttat gctttatata agccattgtt tcacctcatt
    10501 tctattgcat gattaacgta tcctgttctt ctacttgaga gtgacttgtt ttttccgttg
    10561 tttgtaatgc agcggtcatt tcttcaaaca aactgtgtac attgagtttt cctacacgtc
    10621 catagggatc taacatccaa cactgtcctt taatcatgtg gctcatttca gctaagttcg
    10681 cttcatttac ttccaaacgt aaatgattca aaatcttttc tcgctcactg tcttcatcaa
    10741 aagcaaagac gacaccaaaa ttcccttttt ctttatctga acctaagtca ccaataaatt
    10801 gtgtatctaa cactaattga ttcatctggg aacgccccac acgtgccatt gctgcaataa
    10861 tactacgtcc tgcgttcgaa ttattaaata cccatgcttc tgtaaaataa atcgctgttt
    10921 tcttactact atctcgtttt ccaaacattt cacaaaaacg acctaaacta atcatcaaac
    10981 acaaagactt tcgatccgca tctgtatata gttctggtcg gacattgtca tttggtaatt
    11041 tcaaatcttg gatctctaaa atcgttgttt tggcattaaa gtctaatccc gggttttctc
    11101 cataagaaaa tcctaaacgt aaaatcgaat cttctaccgt caagcgtaaa aactcaccat
    11161 attctcgaac ctctttttgt tccatttgtt ctaaatagtc gaccacatgc atcaagccaa
    11221 cttgttgtcc ggcttctttt tgaactaaaa cttcttggat tccctttaat accgcccctt
    11281 ttaaaaggtg attggtacct aacggactta actcatcaat catatcctgg gctacttgtt
    11341 tcgcatctac tcgggataaa caaacaattg gatctaatac accataatta cggggatttg
    11401 tagcatctaa cgtgacataa ctcatttttt gcaaatgttc tacaaacaaa gggtaatgtt
    11461 tctgataata cgggtcttga atgagtttcg taaaccattt gcgcttttct cctttgggat
    11521 ccacaaataa actttgtaca ttcataaacg aactatacaa aaagagcaag ccaactaaaa
    11581 aggtcttccc tttaccggtt tttccagtta ctgctacatg aggcgcatca aacaacgcac
    11641 ctttgattcc ttccgcaatg gctagaagat tcaaaaagac aaaacgattt gacgcataaa
    11701 ttaattcttc taatggtgtt ttttctcctc gagaaccacc atcccctaat gtatcttgcc
    11761 gaccaatata ccagccggat tccataccta attctgatac tgctgaaaac aaccattcgc
    11821 caactgcttc tacagttgtt cggtgcttcc aatgtaactg ccgtcccaat ggttgcccta
    11881 ataaacattt atagaacaat tctcgttgat cattttttgc acggtggata tggataatcg
    11941 tatgaagttg ttgaataacc gttgcactat tttgacgaac ttcttcaacc gtttgaccgt
    12001 atacaataaa acatgcgatc caatcgacga caggttgccc attatctact cgttgcttta
    12061 aatgttttac taatcctcga acagttaata agcgttcact aggttcttca tcattttgaa
    12121 ccaattcctt atgctctgtt ttgaaccgtt ccgatagcca attcagtttt aaaccaatac
    12181 ctccctgtcc tttcaagcgt ggatagtgaa ttttcattcg caattctgct gggaaagaaa
    12241 gatcctgagc aaattgaaat agatcacaat ttctcatatt ttcaggtgtt tccccaatag
    12301 gcaacaatgc taaataacta ctccctaact catcttctaa ttgaagaagg cctttatgtt
    12361 ccttcggtat acgtacatac gttccttgat tgtctttcag tacttcccaa ttattttgtt
    12421 cttctacttt gtgagccatt cctcgaatat attgcagacg attgagatac aacagctctt
    12481 tttttactaa gggacgaata gaaaagctat aaagtaattg tgtcacttct tttacataag
    12541 gatcactttc agaaaacaaa gtttccaaat catctacttc taatccaaat aagcgtgcta
    12601 aacttcctgc taaatctaac cagattagat ttattacatc atgaaaactc tccaccgttg
    12661 ctttttcttt gactttgatt cctaaaataa atgaaggacg atagacttga ccttctcgtt
    12721 ccaacaatgt cactgtttct tttgcataat acgcaccaat tgcttgacta tcgggagaaa
    12781 atccctttcc taatgtgtca aacttcgtcc gtaaattcat tgttcgtgga tacatcacca
    12841 actccacatc ttcataatct gctaaaagca acagtaaacg agccatcatt tcttttattt
    12901 cctgtgtctt ttttggtgtg ttttttgaaa ttgttcgtgg aaaaacatga tagtaggccc
    12961 agatacaatc ctctccattt aacaaacaat ttttcttgaa tgcttttact ggttctttta
    13021 caagcaccaa tgaatttcac ctcccaataa aaaaggaaca accagttgat tgtccctttt
    13081 tctaatcctc taaataaaca tacttattca caaaaagtaa ttcttccaat gcattgtttg
    13141 ataccttgtc ccagttataa tacacacaat caccattcat catacgaatc atataacaaa
//
LOCUS       DAZKKW010000073        13178 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.075, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000073 DAZKKW010000000
VERSION     DAZKKW010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13178
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.075"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            143..1882
                     /gene="yfcC"
                     /locus_tag="ACG8PO_002057"
     CDS             143..1882
                     /gene="yfcC"
                     /locus_tag="ACG8PO_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HFQ0207358.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            2039..3223
                     /gene="iadA"
                     /locus_tag="ACG8PO_002058"
     CDS             2039..3223
                     /gene="iadA"
                     /locus_tag="ACG8PO_002058"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFQ0207359.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            3334..3537
                     /locus_tag="ACG8PO_002059"
     CDS             3334..3537
                     /locus_tag="ACG8PO_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFQ0207360.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(3638..4300)
                     /locus_tag="ACG8PO_002060"
     CDS             complement(3638..4300)
                     /locus_tag="ACG8PO_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0207361.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(4313..5815)
                     /locus_tag="ACG8PO_002061"
     CDS             complement(4313..5815)
                     /locus_tag="ACG8PO_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0207362.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVTVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            6363..8042
                     /locus_tag="ACG8PO_002062"
     CDS             6363..8042
                     /locus_tag="ACG8PO_002062"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HFQ0207363.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            8169..8648
                     /locus_tag="ACG8PO_002063"
     CDS             8169..8648
                     /locus_tag="ACG8PO_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HFQ0207364.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            8867..9547
                     /locus_tag="ACG8PO_002064"
     CDS             8867..9547
                     /locus_tag="ACG8PO_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFQ0207365.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            9555..10886
                     /locus_tag="ACG8PO_002065"
     CDS             9555..10886
                     /locus_tag="ACG8PO_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HFQ0207366.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            10901..11863
                     /locus_tag="ACG8PO_002066"
     CDS             10901..11863
                     /locus_tag="ACG8PO_002066"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HFQ0207367.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            11974..12312
                     /locus_tag="ACG8PO_002067"
     CDS             11974..12312
                     /locus_tag="ACG8PO_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFQ0207368.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            12391..13098
                     /locus_tag="ACG8PO_002068"
     CDS             12391..13098
                     /locus_tag="ACG8PO_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HFQ0207369.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
BASE COUNT         4197 a         2322 c         2888 g         3771 t
ORIGIN      
        1 cataggagaa aaaaaacaaa atagaaaaaa gtgattttct aataaattat tcatgtggaa
       61 atgtcatttt tactgtataa ttcatttgat tatccatttt ttattcatca aaattactga
      121 aaaaagaaag gaagaaataa atttgaagaa aacaaattct tcaggaaaac cgaaacgcga
      181 cttcactaaa ttatcgacac cccatacgta tgtcattatt tttggtgtag tcatctttgc
      241 atggatactg acttttgtcg ttccagcagg aaagttcagc acgcaagata ttgaatacaa
      301 agatgcaaat ggcgaaacaa gtactcgtac tgttttacgt caagattcct tccgttatgc
      361 ttacgagctg gataagtcgt atgtgtttga tcaattagaa gaactgcaag atcatccagc
      421 agaacgtgaa aaactggatg ttccggaaaa aggattggaa aaagtcattg ctgacggaga
      481 aaaaaatctt acgcaggaaa aactagatga aatctccttg acagatgatg tgctttacga
      541 tcagtacggc gaaaatatct atgatacttc taaaaaattg cataaaacag ccaaaatatg
      601 gggaacagat gattttggcg gctttggttt cttaaatttt gtttttgaag gactagtatc
      661 tggagataag tatggatctg ctgtagggat cgctgcattg atccttgttg ttggaggtgc
      721 ttttggtatt attatgcgta ccggagcgat cgacgcaggt atctatgcgt ttatcagtaa
      781 aactaaggga ctggagcgtt tagctttacc gctattattc tttgcttttt cattcggtgg
      841 cgctacattc gggatggcag aagaggtaat tccattctca atggtgatgg ttccatttgt
      901 cattgcgtta ggatatgact cgatcgtagc agtaaccgtg acttatgtcg catctcaagt
      961 cggaaatgcg acctcttgga tgagcccgtt tagcgtagcg gtggctcaag ggattgctgg
     1021 aatccccgtg ttgtctggtg cgacattccg attgattatg tgggtagttg ttactgcatt
     1081 agccgctggt tatatgatga tatatgcaga aaaaatccgt aaaaaaccag aaagttcgtt
     1141 gacttacaaa tcagatgatt atttccgttc tcatatcaaa aagacatctg atgaaaataa
     1201 aaaattcatg cttgggcata aattgatctt attagagatg ttagttgtcc ttgtctggat
     1261 cgtttggggc gtgactcaaa aaggctatta cattccagaa atcgcttctc aattctttgt
     1321 gatgggatta gttgctggaa tcattgctgt tcttttcaaa ttagatggaa tgaaagtcaa
     1381 tgacatcgct tcttctttcc aatctggtgc agctgattta gctggtacag caatcgtagt
     1441 aggtatggcc aaagggattc ttcttgtctt gggaggatct gatgcatctg ttccttcagc
     1501 tttgaatacg attttgcatg ggatcgggac ggcgttgacc ggtgttccag ctgtcattgg
     1561 tgcttgggcg atgtacatct tccaaagttt gttcaacttg gttgttacat caaattcagg
     1621 acaagcagct ttgactatgc caatcatggc tccactggca gatttagtag gtgtatccag
     1681 acaagtagca gttcttgctt atcagcttgg ttcaggtttt atggacgctt ttacacccgt
     1741 atcagccagc ttgattggtg tgctaggtgt tgcacggatc gaatgggcaa aatgggcaag
     1801 attccaaatc aagatgcaag gattcctgtt tgttttagga actattttca tcatgattgc
     1861 cattgcaata ggtctgcagt agtactggag acctgtaaat aaaaaagtaa tccaaacagt
     1921 agataaggac tccaagaacc gtcattttac ggtaaaatca aaggagtaaa gatcctatcg
     1981 tttggattat tttttaagat cctatcatca gttatactta aagaaagagg aaaaatgtat
     2041 gaaaatcatt cgacaaattg aagtgtttgc accagattac ttaggaaaaa tggatgtact
     2101 gattgccggt gggaagattt tggctgtaga agaacagcta gaaggcggct acgaaggagt
     2161 ggaagtagaa gaactttcag gtgagggaaa agtactaacc ccgggtttca ttgactgtca
     2221 tttccatatt ttaggtggag gaggagaagg cggttatcaa aatcgaacgc cagaagtcac
     2281 attgagccaa ttgactacag ctggtgtaac gacagttgtc ggttgtttgg gaacagacgg
     2341 agaaggtaga gatatgactg cgttgatcag caaagcgaaa ggtctggaag cagaaggaat
     2401 ctccacttat gtatacgaag gttcgtaccg attgccggta aaaacagtaa cagattctat
     2461 catcaaagac tttttgacaa ttgataaaat tatcggaatc ggagaaatcg cagtatccga
     2521 tcaccgttct tctcagccaa gttttgaaga gttcgctcgt gcagtagcag atgctcgtgt
     2581 aggaggcatg ttatccggaa aagctgggat catcaatgtc cacttaggcg gaggaaaaag
     2641 aaagctagag ctattgacac gaatcgtaga agaaacagaa attccgatca cacaattttt
     2701 gcctactcat gcaaatcgca caccagaatt atttgaagct tgcgtggcat ttgccaaacg
     2761 tggtggcacg attgacttca ctgctagcga agatcctgat ttttgggaga agacagatgg
     2821 tgaagttcgc ttcagcaaag cactgaaacg attgatcgag gaagatgttt ctttagataa
     2881 tttcacgatg acctcagatg gacaaggaag cttgccttat tttgatgaaa acaatcattt
     2941 cctagggtta ggagtaggaa gtgccaaggc attgctagta gggatcaaag aagctgtgca
     3001 aaaagaaagt atccctttgg aaatcgcctt acgagctata accagtaatc cagcacgtat
     3061 attgaaattg gataaaaaag gaaaaatcga aattggtgct gatgcagatc tgtgtatctt
     3121 ggataaagaa acgcttgata ttgatacagt tatcgctaaa ggagaaataa tggtccaaga
     3181 aaaagaagtc aaagtatggg ggactttcga aaaaagtttt taatcaacag ctctaactat
     3241 cttttttaga aaacgagcgt tctgtttgtc tgtatctgtt gttgatgtta ccctgaaggt
     3301 agatcaataa acaaactaag aggaggaagc acaatggcag atttaaaagg acgttctaaa
     3361 gatatgaaag ataaagtagt cggtgaagga aaagaagcat acggcaaagc aacagacgac
     3421 aagagcaaag aaacagaagg gaaagcacaa tcactaggtg ctgacatcaa agaaaaagct
     3481 cgaaatttag gcgacgaagt caaagacaag ttttctcgaa aagacgaaaa aaaataaaaa
     3541 ccacttatta ctaattgaat caagcaaata aaaaatccga accatatctg cgagacagat
     3601 atggttcgga ttttttattt gatcattatc ctgtttttta aacttcgatt ttttgaaatt
     3661 ttttattttt tagttcaaag acttcatcac agctttttgc tacttcaatc tcatgagtca
     3721 cgatgataat acagcgattt tgttcatggg cgatttcttg gaaaagtttg acgatatctt
     3781 gtgaagttgt ttcatctaga tttccagtag gctcatccgc tacgattagt tgatgttcac
     3841 agcagatagc acgaacgata gcgactcgct gctgctgacc tcccgaaagt ttggtcacct
     3901 gtttttttgc taatatttca tcaattccta cacgagccag tcgatctaaa gcaacttgtc
     3961 gataattctc ttgcttggtc ttagtgattt ccattgctgc tatgacgttg tctagagccg
     4021 ataaataagg aagcaagttg taagcttgga aaataatcga gacatcattt ttacgataac
     4081 ttcttaaccc gattttgttc agtgaacgat cttgatagta tacctttcct tcttttggtg
     4141 catctagtcc agaaataagc gaaagaaaag tcgtttttcc agaaccactt gctcctaaaa
     4201 tagcgtacat ttttccagct tcgaaattca gttctacatc tttataaagc gcatcttgcg
     4261 gatcttgata ccaaaatccc aattgttctg tccgtaacat caaattcctc ctttagttta
     4321 ataggatttt tcttgggttt aatcttaata tccctataga agctaaaaag acagaaaaca
     4381 gactgattcc aaatgctact ccggctaata gtcccaattg tgcaggtttg acattgattt
     4441 ccaattcgct gacttgatca gaaacagcaa atggattatt cgtggttcct tgttgaccat
     4501 cgcgttgacc gccttgttct tgtctgttac ctggcatttg tccaccgcca ggagcttgct
     4561 ggttttgtgt cgtggtttct gtctgttggt caagtagctg ctgtcccacg gcattcgcga
     4621 cgacgtttcc gctaagcgaa gcaactccta atgcaagaat catacaaata gccacttcgg
     4681 taaacaattg gagaatgatt ttacttctgg attctcctaa agatagcaag acgcctaact
     4741 catgccgacg ctcccgaatg gtaatcataa tgatcagagt aagtatgata ataccggcta
     4801 cagtcactag taaaacaaca tttttagaaa aacttgccac gttgtttaaa ggttccaaca
     4861 ttgactgata catggaatca tttgattgta agctaaatgt atctgtgtcg atcagcttct
     4921 cagcttcttt gagaaactca tccattttat ctggatcaga tagagtgtaa atagcagaat
     4981 ctatcgtgtt atcgtcagat gtcccattta gttcatttgc aaaagtgtaa gaagtgtaaa
     5041 gtgtattcga cggattcatg aagttgaatt tcatacccat actgccgctt gtttcgctgg
     5101 attcataaat tcctttgatc gtcatttcat aagtggtatc ttcatcttca gtactcgtga
     5161 ttgtgaaagt atctccgact gccaggtcat ttgtttctgc tagagtagaa tcaatgacta
     5221 cattgtttgt gcctttgtcg ctgtcatcta ttccttcacc atccgtgatt tttgctgttc
     5281 catcagaaaa attactattt tgtgcagttg ctgacacacc actgatttga aagtctgctt
     5341 gagtcatttg cggattcccg cccattcctc cagcaggttc accgcctgca tcagtagttt
     5401 ctgaactgtc ttcacttgaa tcagaactag tgatggcagt tattccatct tttgccatag
     5461 ctgaggcgga actttcaaag gaataactct ttacattatc taattcagct attttttccg
     5521 cgtccgttaa ggaaactggt gtaagagaaa aactgcctgg atctggacgt tcttcagtgg
     5581 tagaaccgct tgaagaagtt tgttcctgtt ttttaaatga agcttctcga ttggcactta
     5641 aagtcactgt tgctccaact tctttttgtg cttgattcgc tgcttcttct gcagcgcttc
     5701 gaatcgtgag gccagctagc acaaaaataa gaatggctgt gaacacggca aacaataaca
     5761 atgaacgtcc ccattttact tttgtacttt tccatgcacg tttcataaaa ttcatatttt
     5821 gcatccctcc tagtagatgc atttgataat agacaaccta tcttaaagct agcttaaatc
     5881 ttatgctgtt tgcacaccat caacatgagg ttctgtatat ataaaaagtc actatagtta
     5941 ttgcaaaaaa tgtggtaaaa tgaaaaaagt atgtttcaaa ccgacgaaga agaaatgaaa
     6001 gaggaatagc aaaagaaatc ctagatgcta cagagggatt tttacgtgca tagttgttct
     6061 aacaattgct gatactagcg atttagttta tttgttgtat aataatgggg aacagctgaa
     6121 tacttatata agtattctgg ctatagcgtt tcgcaaaaga tccattttgt taatgaagta
     6181 tatccaaaat aaaatgccca tccaactgtc agaacgcgga tgtgctggta attttcctgc
     6241 tgattctcca taagttgaga aatagtgatt ttgcatatgc ttcattggaa tggctcatat
     6301 atttagataa agaagaaaat cgacatcttt aaacttggat gtcataagaa gaggtgaagt
     6361 ttgtgagtac cattaaaatt atcccattag gcggcgttcg tgaaaatgga aaaaacatgt
     6421 atgtcgcaga agtgggcgag gatattttta ttttagattg tgggctaaaa tatcctgaaa
     6481 atgagttgtt ggggatcgac gtcgtaatcc ctgattttac gtatttagaa gaaaacattg
     6541 atcgtgtagc tggtgtcttt ttaacacatg gacatgcaga tgcgatcggg gcacttcctt
     6601 acttgttgtc aaaaatttct gtaccagtat tcgggacaga gttgacgatc gagttagcaa
     6661 aattgaatgt cagtcgtaat gaagcagcca aaggatttaa ggatttccat gtggtagatg
     6721 agcatacaga aatagacttt ggtgaaacag tagtgagctt tttccgtacc actcatacga
     6781 taccagattc agtggggatc aatctgaaaa caaaagaagg tagtatcgtt tatacgggag
     6841 attttaaatt tgaccaaacg gctatcccaa tgtatcaaac agattatgca cgattggcgg
     6901 aaatcggcaa agaaggagtc ttagccttac taagtgaatc ggcaaatgca gaaaatcctc
     6961 agcctgtagc ttctgaagcc caaatagcag atgaagtatt tgatacgatc aaatattggg
     7021 aagggcgtat catcgtcgct tgtgtagcaa gtaatttgca acgagtgcaa caagtattgg
     7081 atgcagcaga aaaagcacaa cgaaaagttg tcttgactgg tcaagatttc gaacgaatta
     7141 ttcgcacagc gatgcgttta gaaaaacttc aattaccaag tgaagatctg cttgttacgt
     7201 tgaaagacat gaaaaactat gatccagaag aactgatcat tttagaaact ggccgaatgg
     7261 gtgaacccat caagtctctg caaaaaatgg caaacggtac acaccgtaat gtcaagattg
     7321 aagaaggaga tttggtttat atcactacta cgccaagcat cgcgatggaa accatcgtgg
     7381 cgaaaacgga agatatcatt tatcgagctg gtggaacagt gaagttgatc tctgagaata
     7441 tgcgcgtttc tggccatgcc aatcctaatg acttgcaatt aatgatcaat ttgatgaaac
     7501 caacatattt tgtgccagtt caaggagagt atcgtgaatt agctgctcat gcagatttgg
     7561 ctcatgcagt agggatgcca tacaaaaata tctatattac tggacgcgga gacgtgctgg
     7621 aatataagaa caagcgtatg agtgttgcag gaactacaag tgcagaaaat gtaatgattg
     7681 atggattagg cataggtgat atcggaaaca ttgttttacg tgatcgaaaa atcttgtcag
     7741 aagacgggat tttcgtagcg gttgtcacta tcagtcggcg agaaaagaaa atcatttcaa
     7801 aacctcaaat tacttcacgg ggattcgttt acgtaaaagc tagccgtgat ctgatgaaag
     7861 aaagcagcaa tatgattgaa acaatcgttg agaaacattt agaaagcaat gattttgagt
     7921 ggagcaaatt aaagcaagac atccgagatc aattaggccg ttacttattt gaacaaacaa
     7981 aacgccgccc agtgatcttg ccagtcatta tggaagctac tcaacgtcga aataaaaaat
     8041 aaacaaaaag agtaagatga acctctctat agagtgctcg tcttgctctt tttttgcgaa
     8101 aaacaaataa tttttgaata cagcatccct cgctttataa taaacgatag aataaaggga
     8161 gtgaatcaat gatgagtcaa acaaaagtcg aacgttattt agaagaacaa aagatcagct
     8221 atcaaccact tgattttagt catgtggtag aagaatcttt cacagaagaa ctacaaaaaa
     8281 gagggattga tccagctttg atatgtaaaa ctctcgtagt gaaaggaaac aaaactgggg
     8341 tgatcatcgc attgattcct ttgaacgatc acttagatta caaaaaaacg agacaagtga
     8401 cgaaagatcg taaagttggt tttcctggaa tggattttgt attaatgcat acaggatatc
     8461 cccacggagc aaacacccct attggaataa aactcgcaca tcctgattat tgttttttag
     8521 cagacagttc tttaaaagaa aaagaagaag taattgtgtc aagcggtgaa atcgggagaa
     8581 gccttcggat aaaagtagcc gatttagaaa agatcgtcca tcctatttac gctgatttaa
     8641 ttagttaagg gacgagaaga gaagctaagc agagcagaga cctcaaactt gagattatat
     8701 ctgcttggta cttggtgcat cctgtattat ctgttacaat tgctttgtac atactgtact
     8761 atcaatccaa tgaaaatata tttagcagct atattttttc attgatgtgc aagtgatgaa
     8821 atcgttttta ataaaaaaga tacagagaaa acgcgaggtg taaaatatga aaattacgta
     8881 tcatggacat tcctgtttaa caattgaaat ggaaaatggc caacgcgtga tgattgaccc
     8941 gtttattaca ggaaatccat tgacagactt aactgcagaa gaagtaaaga cagattggtt
     9001 gctgattact catggtcata gtgaccacat aggagatatg attcctattg caaaagaaaa
     9061 tcaagccaca attatcagta ttgttgagat tgctgattat gcgaacagca gaggagtaga
     9121 ttcattcggt atgaatatcg gtggaaaaca tgcgtttcca ttcggcgtgg taaaattcgt
     9181 acatgcacag catagttcaa gctatgaagt agacggtatc gttcagtata tgggcgagcc
     9241 tagcggaatt attattcaag cagaaggaaa aacgatttat catgcagggg atactgctta
     9301 tttcagtgat ctaggattgc ttgcagaaga attcgatatt gatgtcgctt tcttgccgat
     9361 tggcgataac tatacgatgg ggccagaaga tgcagcaaag gctgcgcaaa caattcgtgc
     9421 caagaaagta gttccaatcc attacaatac tttccctgtc attgaacaag atcccgaatc
     9481 ttttgtagag ttactggaag atagtgaagg aaaaataatg agcgtctcgg aaacgctgga
     9541 agtgtaggca gaaaatggcg acaaaacatg atttgatttt agaatatatt gaaagtttac
     9601 ctgttggcaa tcgaatttct gtaagaagca tcgcaaaaga attgaatgtc agtgaaggaa
     9661 cagcgtaccg tgcaatcaaa gatgcagaaa atgtcggact agtttcaaca attcaacgag
     9721 tagggaccat ccgtatcgaa cgcaaattaa aaaaacatat tgaacgtttg acatttggcg
     9781 aggtcgtcag gatcattgaa ggggacgttt taggcggggc ggcaggatta gacaaggtct
     9841 tgaataaatt cgtcatcggc gcgatgacta aacatgccat ggaacgctat attacgccag
     9901 gatcactaat gatcgtcggc aaccgggcgg aagtccaaaa attagcattg gaagatggcg
     9961 ccgcagtatt gatcacagga ggattcaata cgactgctga gattgcaaag ctagctgatg
    10021 atcttgaaat gccgatctta cgcacctctt atgatacatt tacagtcgca accatgatca
    10081 accgagcgtt aagcgaccaa ttgatcaaaa aagatatcat gcttgtcagt gatatctata
    10141 tgccagctga aaagactcat tatttgcagc agacagatac gatttctgat tatcagcgtt
    10201 tgtcagaaga aacgcagcat tcgcgttttc cagtcgtcaa ccgccatcat cgtttgatgg
    10261 ggattgttac ggcaaaagat gttttaggga aatcgccaaa ccagatcatc gatcgggtga
    10321 tgacgaaaga accaatcagt gtaaagaaaa caatgagtat tgcctctgtt agccatcaaa
    10381 tgatctggga tgggctagaa gtcatgccgg tcgtttccga tgacttgaca ttggaaggct
    10441 tgattacccg gcaagatgta atgaaagcga tgcagcttgt ccagcgacaa ccgcagattg
    10501 cagatacgat ttcggatcag atatctggaa atatccatac gattgatact gaccgtgaag
    10561 gaaatcgttt ggaaaaccct aagttcaaat tcgtagtagc accacaaatg gtcaatggcg
    10621 ttggaaccat ttcttttggc gtacttagcg aaatcatttc tgatgtcgca caaaaaacaa
    10681 tggtgatgaa ccaaaaacga aatatcttga ttgaacaagt gaacctacat tatttgcggc
    10741 tgatccaatt ggaaagcgaa ttagacattc gtccacgtgt attggaaatc ggcagacgtt
    10801 ccgctaaact ggatatcgaa gtgtttattg aaaacaccat cgtttccaaa gcaatcgttg
    10861 tttgtcaagt aatggaacgt tcttagaaag caggagtgaa atgacagtac agcaagaaat
    10921 agtagataca ataaaaaaat acgataaaat cattatccac aggcatttgc gccctgatcc
    10981 tgatgcatta ggatcacaag taggattagc cgaactatta cgggaaagtt tcccagaaaa
    11041 agaaatacgt caagtaggtg aaacgattga agggcttcgc tttttagcag aaatgcaaga
    11101 agttgaagat gtcttctacc aaggagcgct agtcatcgtg actgatacag ctaattcacc
    11161 cagaatcagt gatcaaagat atcagttggc agaaaaactg atcaaaatcg accatcatcc
    11221 caatgatgac ccttatggag atcttgtgtg ggtaaataca aaagcaagta gctgtagtga
    11281 gatgattgca gaatttgctt tgatgtttcc tgaatcttta aagatgaacg ttaaggctgc
    11341 acggttactt tatgcaggaa tcgtcggaga tacagggcgc tttttgtatc cctccacgac
    11401 gtcccgtaca ttagagattg cttcgatatt gagaaattat ccatttgatg caagtgcttt
    11461 gaacagagag attgaacaaa tgccgatgaa agtcgctaaa ctttccggtt atctttatca
    11521 aaatatccag gtggatgaaa atggtgcagg aagaataatc cttccgcaag aaattcttga
    11581 taaatatgga atcgatgatt cagagacagc ttcgatcgtt tcacttcctg gagtgattga
    11641 cgaagtgctg gcttggggga tttttgttca gcagccttca gggtattacc gcgtacgttt
    11701 acgttctaag gggccgatca tcaatgaatt ggctaaacgc catcatggag gaggccatcc
    11761 attagcaagt ggtgcgaatg caaaagattt agcagaagta gaggaaatct ataaagagat
    11821 ccaagatata tgccaatcgt ataagagcag aaaaacagaa tgatacttcg ttcgaaaacg
    11881 tgccttttgt agtaaaaatc acaaatgtat cttttcaaga tcttcttttt ccgttacaat
    11941 agtgctcact aaagatttta aaggagaaag aagatgacat taccaaattg cccagaatgt
    12001 ggctcagagt acacatatga agatcgaggc ttgttgattt gcccagaatg cgggaacgaa
    12061 tggaacaaag aagaacaaga aactgtcata gaagacggat tagtggtatt agatgcaaat
    12121 ggaaaccgtc tgttagaggg cgatagcgtg acagtgatta aagacttgaa agtaaaaggt
    12181 gcgtcaggtg ccattaaaca aggaacaaaa gtcaagaaca tccgtttagt agaaggcgat
    12241 cataatatcg attgcaaggt agaaggattt ggaccgatga aactgaaatc ggaatttgtg
    12301 aaaaagaatt aaagaatatt caaggtcttt tcacattctt ttcctactac taatgataaa
    12361 ataaagtaaa cgaaaaggag tgaaacgaaa atgtatggtt atggaatgta tggtttttta
    12421 gatccgacat ttcttttggt cattcttgga ttagttattt ccggaattgc gtcagcgtac
    12481 gtcaatagta ctttccgaaa atacgatcaa gtgagaagca aaaacaatgt gactggtaca
    12541 caagcagcac aatatatatt acaaagtcaa cagataaata atgtcggtgt tcaacaaatt
    12601 gccggagatc tcacagataa ctacaattca ggtaataaga tgttgagttt gtctcaagca
    12661 actgcacagt ctacttcagt tgctgcgata ggtgtagcag ctcatgaatg cggacatgcg
    12721 gtacaagatg cttcgggtta tgtcccattg aagattcgtg cagcacttgt tccagcagct
    12781 aactttggtt cgatgatttc gttccccttg attatggtag gtgtattgct tagctggaac
    12841 tctacgttaa tcaatatcgg gattttcgct ttttctttag cattactttt ccaattggtg
    12901 acattaccag tcgaatttaa cgcgtctcgg cgagcaatcc aaattttgag tgaaggcgga
    12961 ctactgacgg aagaagaagt accgatggca agacacgtat tatttgcagc agcactgact
    13021 tatgttgcag cagcgctttc gacattcctg cagctgttgc gcttgatctt attgttcggc
    13081 ggcaaccgaa gagattaaaa taaagaagtt gtgaaatcag cgctttgatc tgagctaata
    13141 ccgagagtta gctgattgaa taccatttat ttggaata
//
LOCUS       DAZKKW010000074        13172 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.076, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000074 DAZKKW010000000
VERSION     DAZKKW010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13172
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.076"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(133..808)
                     /locus_tag="ACG8PO_002069"
                     /pseudo
     CDS             complement(133..808)
                     /locus_tag="ACG8PO_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
     gene            complement(818..1633)
                     /locus_tag="ACG8PO_002070"
     CDS             complement(818..1633)
                     /locus_tag="ACG8PO_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0207370.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTYTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            complement(1895..2338)
                     /locus_tag="ACG8PO_002071"
     CDS             complement(1895..2338)
                     /locus_tag="ACG8PO_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HFQ0207371.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     LSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            complement(2472..2810)
                     /locus_tag="ACG8PO_002072"
     CDS             complement(2472..2810)
                     /locus_tag="ACG8PO_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFQ0207372.1"
                     /translation="MYQVKKSRSIAMELRLLKYFWTIAEEGTISQAAEVLHLTQPTLS
                     RQLKELEEELGTELFVRENRKMILTEAGYFLKKPSRRNFRFNESNHSRIREQKKTIIQ
                     RPIYDRLCGS"
     gene            complement(2798..3175)
                     /locus_tag="ACG8PO_002073"
     CDS             complement(2798..3175)
                     /locus_tag="ACG8PO_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFQ0207373.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKKIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            3399..4592
                     /locus_tag="ACG8PO_002074"
     CDS             3399..4592
                     /locus_tag="ACG8PO_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0207374.1"
                     /translation="MTTYQRKLLLSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFIMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            complement(4758..5180)
                     /locus_tag="ACG8PO_002075"
     CDS             complement(4758..5180)
                     /locus_tag="ACG8PO_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0207375.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQKYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
     gene            5575..5769
                     /locus_tag="ACG8PO_002076"
     CDS             5575..5769
                     /locus_tag="ACG8PO_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0207376.1"
                     /translation="MVAGSLLSGCGATTSTTQSTSSDQASSSSTTISESTADSSKEQE
                     QIEKKKEEKQKAEKAALEKK"
     gene            5766..6260
                     /locus_tag="ACG8PO_002077"
     CDS             5766..6260
                     /locus_tag="ACG8PO_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0207377.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQEAIAQQQAEQARQQSA
                     AEQASQQAQAAAPEQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLSEAQARGLTPCSK
                     CYGN"
     gene            complement(6422..7594)
                     /locus_tag="ACG8PO_002078"
     CDS             complement(6422..7594)
                     /locus_tag="ACG8PO_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFQ0207378.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEESSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(7800..8615)
                     /locus_tag="ACG8PO_002079"
     CDS             complement(7800..8615)
                     /locus_tag="ACG8PO_002079"
                     /inference="COORDINATES: protein motif:HMM:NF033902.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFQ0207379.1"
                     /translation="MTGFDENTGVALYDPAESALTFTTDTEGSISVNGLIDGNYTLVE
                     TKAPDGYQLTNPQKETTFIVKNGKLQQTTGIIHGADEANLGNPNGVVNRENTTTDNTI
                     TVENVQNYGDFHFIKQDVNTQEKLADAEFSILRAKDPTVKLMKKIANLASGEYPYAWS
                     DEVTGSDWEEVKLISDSEGKFSIEGLKQGAYCLQETKAPEGYALPKNEAAYTEFTVDD
                     DPTTSDETTIDNQPKGILPSTGGMGIIVFILVGTALVGGAVIYFKKRHQETEA"
     gene            complement(8765..9211)
                     /locus_tag="ACG8PO_002080"
     CDS             complement(8765..9211)
                     /locus_tag="ACG8PO_002080"
                     /inference="COORDINATES: protein motif:HMM:NF027869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
                     /protein_id="HFQ0207380.1"
                     /translation="MKNIKTKIWTFLGTAIMLLPFVLGLGTAEVSAAVSPTPENVTVN
                     LHKLKFTSAPENQINNGTELTFPNSEPLNGVEFNVYDITATYYPSKDTAVPADATPFA
                     SVTTSGEGLANLTLPGKSDGKDAVYVFVETPKPGVETSPNIVLSLP"
     gene            complement(9208..9723)
                     /locus_tag="ACG8PO_002081"
     CDS             complement(9208..9723)
                     /locus_tag="ACG8PO_002081"
                     /inference="COORDINATES: protein motif:HMM:NF037639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prealbumin-like fold domain-containing protein"
                     /protein_id="HFQ0207381.1"
                     /translation="MDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFT
                     SSKDGTFSVHGLSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEP
                     KANPTNNGKTPVQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKK
                     QQKGNNRREIK"
     gene            complement(9796..10710)
                     /locus_tag="ACG8PO_002082"
                     /pseudo
     CDS             complement(9796..10710)
                     /locus_tag="ACG8PO_002082"
                     /inference="COORDINATES: protein motif:HMM:NF027869.4"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
     gene            complement(10707..13034)
                     /locus_tag="ACG8PO_002083"
     CDS             complement(10707..13034)
                     /locus_tag="ACG8PO_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBG domain-containing protein"
                     /protein_id="HFQ0207382.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSAIETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDATFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
BASE COUNT         3795 a         2550 c         2368 g         4459 t
ORIGIN      
        1 aaacctgttt cattcaaaag tatagcatgt tttatttcat ttgtttagcc atatgtttca
       61 aaaaatgaaa aagaatgaca gtttttacat aaaaaactgt cattcttaat tgaaagtttc
      121 aatttataaa gtctacagat ccatagttct catcaattta cgattgttga agtaaatcaa
      181 accaaatacg atagaaggac ctagtagaat acaccattgg ggctttacga aaaagaagag
      241 aaacaaactt gtcaatagta aaaaatctag taaactcaca taccattttt tcaacgtata
      301 gtagaacgct atccgtagaa cttgatttgg tttcgacgct ggatttctca cctgaaaata
      361 ccagcagttg atgctaaagg aaactgataa acaagcaagt aaaacaagca agtaaaacaa
      421 acaacggaat caaccatttt ccataagaaa aacgtataaa aaagaaacaa tcaaaaatca
      481 ttatcacact aactatccaa gcaaatagcc aataaaaaaa tccctttagc caaaattggc
      541 gaaacgtctt tatgaacaaa cgtactggtg aaaaactttt ttcttttgct aattcttctg
      601 tcatacctat catcacgatg caacctggac caataaaaag taaagaaaaa ataaaggaaa
      661 agacattcct tccatctatc gacaaaagga acgcagctgc tatatacaag atattgacac
      721 accaaaaaca aaggctactt gccaatgatt gatacaacca ataaaagaat tccatataaa
      781 catttttgtc aaaacgctgc tctttcataa tcacctccta ttccttcatt cctgatgaag
      841 cgatcccttc tacaaaatat ttttgcatcg ctaaaaatat aaaagcaaca ggaagaacag
      901 aaacaactga ggcagccatg attaatgcgt actctgcatc aaatagccca ataaacattt
      961 tcaaccctaa ctggattgtt ttattttccg tagaagacaa ataaatgaat ggccccatat
     1021 aatcgttcca tgtattaacg aatgtgatga tcgtcaaact ggcaatcgcc ggttttgtaa
     1081 gaggtaaaat gatcttgcga tagattcccc actctgataa gccatcgatc cgcgcactct
     1141 cacataaaga atctgggata ctactgtagt attgcttcaa caagaacaca ccaaaagcat
     1201 tgaacgcttg taatagcacc aacgaccaca acgtatatgt taaacccact tttctcatca
     1261 taatgaactg cgggatcata taagactgcc aagggacagc aatcgtgccg atatatgcta
     1321 aaaataaaat atcgcgccct ttaaaattta gtttagaaaa cccgtaagca gcaaaactag
     1381 aagtaaacaa ctgaatcaac gtgatcacaa cgcttaaaaa agctgtattc ttaaaaaatg
     1441 tcactaaagg aatacgagac caaataacca gataattctc taaatgccaa gttttaggga
     1501 tccattgaat cggaatcgtg aacacttcat tatttgcttt gaatgaagca gaaaccatcc
     1561 ataaaaaagg aatgatcgtc aataaagcta atacaatcag cagaatcata cccaaccatt
     1621 gaataaaggg catgtttttt gaacgaattt ttgctttttt tctttcattt cgaacacctc
     1681 caaagttata atcttcattt ctttgtaatg attacaatcc agaacaacgg ttattatagc
     1741 tgattcattt ccgttcttct tatgtttgta agcgcttatt taacaagtta tgtaaatctc
     1801 tttatttttt ttgatttaaa aatgaaaaaa tacaaaagca agactgtcat acgtctcgct
     1861 tttgtattga aatttcttcg attagagata cttattactg ttcaaaagct tctttgaaac
     1921 ggtgaatcaa ttcattgact acaggtgata aaatccgatt ctttttccag actagcatgc
     1981 agttcgtttc aatcattggg tcaagcggaa cgaatgtcac tcgctttgaa agatgactta
     2041 cagcaccttc tatgactaaa gcagcaccta cctgattctc aattagcgca aatacattga
     2101 aaatcaaatt gtacgttcct gtgatcagca actgctcctt ttttatgcct aaccagtcag
     2161 caaacattcg ctgtatttct ggacgctttg agatcaaaag aggtacattc gcaaagtctt
     2221 cttttgttat cacgtctttt tttgctaaaa cagaattttc taaaatgagc aatccccatt
     2281 tttcctttct tggtaacaaa atcgtttcgt atttctctgt atctactgga tctagcaaaa
     2341 tcgctaaatc taacaggctt ttttctagtc actccgtgat atcatcactt gttccgctgt
     2401 ggatatgaaa ttgaacgctg ggataatcac ggatcaactt ttctagtaat agtgccaatg
     2461 tattcgaatt atcagcttcc acacagccga tcgtaaatcg gccgctgaat aattgttttt
     2521 ttctgttctc gaattcttga gtggtttgat tcgttaaatc taaaatttct tctgctcggc
     2581 ttttttagaa aataacctgc ttctgtcaag atcattttgc ggttttcgcg tacaaacagt
     2641 tctgttccta gttcctcttc taactccttc agttgtcggc ttaaggttgg ttgtgtaaga
     2701 tgtagaactt ctgcggcttg agagattgtt ccttcttctg caattgtcca aaaatatttt
     2761 agtaagcgta actccatagc gatgctcctt gattttttta cttgatacat tccattttat
     2821 ttctagataa cacacgatca tatttcttga tttttccttg taaacgttcc aaacattctt
     2881 gcatttcagc aatttttttc gtcaaaagtt cactttcttc aatcaaaagc ttacgccgct
     2941 caaatacagt tgcttctcct tcgctagata ggcgaatata ttcaatcaaa gactcgatcg
     3001 ataatccggc atttcgcata cagatcgtaa agtcgaccca ttttaggtct tcttctgtat
     3061 aatcacggat tccgcctttt gtccgattaa ctggtggaat caacccgatt cgttcataat
     3121 agcgcaatgt atcggccgaa atgccctttt cttcactgac ttgtttgata ttcatctgat
     3181 cactctttct cttaatttct tgccacttta cttatgtatt ttatttattc tacaacttta
     3241 agtgtactcc aagtcaatac tcatttctat taaaactaag ctggattatc tatatcggtt
     3301 gtttgtgtat tatttcaaat aaaaaagtct tgttttttta ttcttttttg gatatacttt
     3361 taacaattta gggaaatttt aggaaaaggg gtataaaaat gacaacgtat cagcgaaagt
     3421 tattattatc gacatcggcg ggaattgctt tagagaatat ggatattatg tttttagctt
     3481 tttcattatc ttcaatgatt acttcttttc atattagtgg tacgcaagcg ggtttcattt
     3541 caacgatcac taatttgggg atgctcattg gaggtatttt ttttggcatc atagctgata
     3601 aatatggacg tgtgaaagta ttttctcaaa cggtccttct gttttcgatt gcttctttat
     3661 tgatgtattt tgcttcaaac atctacttgg tttatctctt tcgctttatc gcaggtgtag
     3721 gagcgggagg agaatacggg gcgtgtatgt ccttgatttc cgaaagtttt tcaaaaaaac
     3781 aaattggacg agcttcttcg gttgctggta ttggtgcgca ggtcggtgcg gctttggctg
     3841 cgattttagc tgctgtgatc attccaacgt ttggttggaa gatgttgtat gtcgtaggtg
     3901 tactgccggt gttgatggtc ttgttcattc gtcgcggctt gactgaacca aaggaattca
     3961 aatctgtaca atctgccgaa aagaaaacca aacttagcag actgtttgaa tcaaaacatc
     4021 ttgcttggca aacatgtggc cttagtatca tggtaactgt ccaaattgcc ggctattttg
     4081 ggttaatgaa ttggctgcct tcaattatgc aaaatcgatt agggctttcg gtttctgggt
     4141 cttcattgtg gatggtcagt actattttag gcatgtcttt ggggatgatg acttttggca
     4201 taatcatgga taaattgggg cctaaactag ccttttcttt gtttttaatt tgttcatcct
     4261 tatctgtcta tctacttgtg ttagcccata gccagtggag tttggtagca gccgctgtga
     4321 tagtcggcta ctttatcaat ggcatgtatg gtggatatgg cgcagtgatt agtagtttgt
     4381 atccaacaga aatccgcgcg acggcgaata atttcattat gaaccttgga cgagctgttg
     4441 gtggtttttc ttctatcgtc atcggtttct tgatggatca ttatagttta actgccgtaa
     4501 tcttgttttt gagcggtatt tacctagtca gtctgctggt cttgacaact cttactggtg
     4561 ttacagcatt aagggaaagc ttgcagcatt gatagttgag tctgtaaatg acaagaaaga
     4621 ggtgtggcaa aagcttgcat ctgcggtaat aagaccagcc aaatcaaaaa catgagacac
     4681 tgtttccgtt tggctggtcg tattacttga tgcagggcag ctttttcaac tgctaatttc
     4741 cttctatctg gcggctacta ttctttgacg tccgtcattt caaagtactt tactaataac
     4801 ttagcataac gaaaaactgt ggatacactc gtatattgag cgtctgcata atcttccaaa
     4861 tttttctcag gatgttgata catgaattca atcaaatgat aattatatga ttttctgtag
     4921 aaatgaagat aaataatgta gattgggaag ttctcctgct tttccataga aaagcgccga
     4981 ttattctcat tcttcaaaat gaccttatca ggatattttt ggaacaattc ttctaaatct
     5041 gttagataat tcaagataga cttacttgat accttcaatt cgttttgtag ttcttctatt
     5101 gaacaccaac gttctgtttc aagcagtaaa tgaatcattt taattttctt ttgagaaact
     5161 tttgttaaaa aagtctgcat attttctcct cctgtatcta ctttactata ctacatacaa
     5221 gaaaaaaaac ggatacaaat atcatattgt cacaccgtca atagtgcata aacctgttat
     5281 tatttctctt tttttcctaa gtattctatt gatatgaatc atttaatcgt ttattatata
     5341 aatgtcaata atctaataat aaaataacgt acatatttta ttattgcata cttaataaca
     5401 gaactatagc aaaaaatccc gtgtatcaac acgggattag attaagtttc ctcaatcact
     5461 attcataaca caattattat aacaagtaag taaacaattg aaaaggacat ttattgaaaa
     5521 cggttgtttt ttacttaagg aggaaattta gcgaaaagat tagttgcatt gtttttggtc
     5581 gcaggaagtt tgttgagtgg gtgtggtgct actacgtcta ctactcaaag cacaagttca
     5641 gatcaggctt ccagcagcag taccacgatc tccgaaagta cggcagattc atcgaaagaa
     5701 caagaacaaa ttgaaaagaa aaaagaagag aaacaaaaag cggaaaaagc tgcactagaa
     5761 aaaaagtgac agaagcggaa tctgctttgt cacaagctga agcaaatcct acaaaagaaa
     5821 actatgcaac tgcttcagca gctatccaag cgctacctga aaacaaacaa gagttgacaa
     5881 gccgtcttgc tgcaattgat tccacaatca aagcaaaaga agcagaagaa gaacggaaaa
     5941 agcaagaaga aattgcacga caacaagcag aacaagcgca acaagaagca atcgcacagc
     6001 aacaagcaga acaggcgagg caagaagcaa tcgcacagca acaagcagaa caggcgcgac
     6061 agcaatcagc agctgaacag gcctctcagc aggcacaagc cgcagctcct gagcaaaata
     6121 atgaacaaac tgtctatgtc acaccgaccg gttcaaaata ccatacacat aaatgtggaa
     6181 atggtacgta tacccctgcc actttaagcg aagctcaagc tcgtggttta accccttgct
     6241 ccaaatgtta tggaaactaa ttcaaaagtt atactataat aataaaattc ttctgaaaca
     6301 taaaaaaata gaagatggcg ttccagatag taaaccaaaa ataacagttt acacgtccat
     6361 ttcgtcagct tctctctttt ttcatttagt ctgttccctc tgcttttttg gacatcttca
     6421 ttcattcttt ctcaatcaaa tagtacacct ctttttcttt tcgcagttta tatcttcctt
     6481 ttttcaattt cattgaaaac tgtttcgaat gtttatgttt tacatggatt ttaagcagga
     6541 gctcctcatg ttgcaagcga tagttcctgc cttttaaatc agaaaatctc actagtccgt
     6601 attcatcaga agctttcaca aatggaactt tttctctagt aatatagtgt ttgccgttga
     6661 gcgtttttac agaaaagacc attccttcga ttggtcgtcc gtttgtgttc tttacataaa
     6721 aagataaagg atagtttcgc ttagcgatgg ccaaagaatc taaataagcg atgagtagcc
     6781 aaactaagcc gattgatcca agtagtatac cgataaacca aataaaaaat ttccattttt
     6841 tccattgatc aattccttgg atcgcttttt ttgcctcttt tgcttgataa ggaatacgat
     6901 gccccgtcac aagcagtcga tgtgtattga ccatataagg agtacaggta agcaaagtca
     6961 cgagatctct gcctttttgg atacccaaag aatctacttc atcaggtaag accacttgga
     7021 ttttttctac ttgatacgcc aatgtctttc cattgatctg gatgaaaaac tggtctcctt
     7081 ttttcaactt aggcaaatct gtaaataatt ttgcttctgg gagtccacga tgcccagaaa
     7141 gaacggcatg ggtgtttttc ccgccagtcg gataagaact ttcttccaat aatgacgttc
     7201 ctttttgcaa aaatatttcc gttgtctgat cgaatatagg taagctgaca ttgatttttg
     7261 ggatttcgat gactccaagc atatgttcca taaaaaaggc attactaggt aaatctttta
     7321 atgcttttgc atcaactgct tggttgaaac tgcctatccc tggtgatacg ttttgctctg
     7381 ccaactgttg gtttttcttt gtcatttttt cctgctgttt ttttatttcc tctgaattct
     7441 tattcgatgc ttgcttttga taatggatga tcatttgctg ggcaagaaaa tcattcagtg
     7501 aatcttctac aaaaggataa gaaaagatcc cgatcccaac gactaaaatg attgccatga
     7561 agatgtccag tattcttatt ctgcttttat tcatcatgtc cacctatttt ctaatatgta
     7621 tatgaagagg ttgtgaaatc agcgccttga cctgagtatt agcgaagcaa acggcaaaat
     7681 agttcctcct attttgccgt ttgttgagcg aataccaaga gaccgaccac ttgtcacaac
     7741 ctttctcccc atttgaatca ctatcgttta tttatcgtat gatttttgtt tccaatagtt
     7801 taagcttctg tttcttgatg gcgttttttg aaatagatca ctgctcctcc aactaaagca
     7861 gtacctacca agatgaatac gatgatcccc ataccaccag ttgaaggcaa gattccttta
     7921 ggttggttat cgatcgttgt ttcatcagaa gttgttggat catcatctac tgtaaattca
     7981 gtgtacgcag cttcattctt aggtaatgcg tagccttctg gtgcttttgt ttcttgaagg
     8041 cagtatgccc cttgtttcag cccttcgatt gaaaatttcc cttctgaatc agaaatcaat
     8101 ttcacttctt cccagtcaga acctgtgact tcatctgacc aagcatatgg gtattctcct
     8161 gaggcaaggt ttgctatttt tttcattaat ttaacagttg gatcttttgc tcttagaata
     8221 gaaaattctg catcggctaa tttttcttgc gtgttcacat cttgtttgat gaagtggaaa
     8281 tcaccgtagt tttggacatt ttctacagta atcgtgttat ccgtagttgt attttcccga
     8341 ttgactacac cgtttggatt cccaagattg gcttcatctg cgccatggat gataccggtt
     8401 gtttgttgca attttccgtt ttttactata aaagtagttt ctttttgtgg attggttaat
     8461 tgataaccat ctggtgcttt tgtttcaact aaagtataat tgccgtctat taatccattg
     8521 acactgatgg atccttcagt atcagttgtg aatgtcaacg ctgactcagc aggatcatag
     8581 agagcgacac cagtgttttc atcaaaaccg gtcacgaatt gattctctga atttttgatc
     8641 tggaattgcg cccctgctaa cttatctacc cctgaaaagt tcccagcttt ctggattttg
     8701 atattgcttt ctttgatgac atttttagca tataggtgaa tcgtgtctaa aggtttgtca
     8761 gggtctaggg taagcttaac acgatatttg gcgacgtttc gacaccgggc tttggtgttt
     8821 ctacaaagac gtatacagca tctttcccat cagatttacc tggaagagtc aggttagcta
     8881 atccttcacc agaagtcgtt acacttgcga agggtgtagc gtctgctgga actgctgtgt
     8941 ctttacttgg atagtacgta gcagtgatgt catatacgtt gaattcaacg ccgttcaacg
     9001 gttcgctatt cggaaatgtc aattccgttc cgttgtttat ttgattttct ggtgctgaag
     9061 tgaatttcaa tttatgcaaa ttaaccgtaa cattttctgg tgtaggtgat acggctgcag
     9121 aaacctctgc agttcctagg ccgagaacga aaggaagcag catgatagca gttcctaaaa
     9181 acgtccatat ttttgttttg atgtttttca ttttatttcc ctcctattgt ttcctttttg
     9241 ttgtttttta taaaaccaag ctgtccctgt tgaaaggact agcaataacc ctaaaattga
     9301 gaaacctcca agcatctctt cgcttgtttt tggaaagctt cctccatttt tttcatttga
     9361 tggaccattt gtctgcatct gaactggcgt ttttccatta ttcgtcggat ttgcttttgg
     9421 ttcgttgatg atttccaatg gtataggatg aagcggatca taactgcctt tttcaacttc
     9481 aaagagaatg gttgattgac tcaatatata tcctttaggc ggtttaatct ccactagttc
     9541 gtaacttcca aaagacaatc catggacaga aaatgttcca tctttactgg aagtaaaaat
     9601 cgttgctctt gaatcatctt tggctaccca atgatttatc ccgttttctt gcgctaagta
     9661 ttggtcattt tttttgacta gaaattgagc atctgctagt ttttgtttcg tttgccgatc
     9721 cactttgaca aattgtttgc ctcctgtttg gatggcaaga tgtgtttcta ctgtttgatt
     9781 gtcagtcgtc aaacgtacat cgttatcaaa tgtcgttttt tcactgtttt ctttcaatgt
     9841 catttggtaa cggatcgtta atttttttcc agcaaacgaa ctaagatctt ttccagaatc
     9901 tgataagaca aaaccatgtt ttattttttg tgtgtgaaat gtatgaatct tgtctccacc
     9961 aatcaaaaaa tcgatcgttt ctggttctag ccataactga ggatcagctg tatctttgat
    10021 cgtataggtt cctatctcgt tgatgttctc aggaattgtt acggtgatga gataagggac
    10081 tttatcccca tctgcaaaat ctgtctgctt attctctact gtttttgtta atgtgagtgc
    10141 agcaggtatt ttttgttcgc tctttggata caagtggatg gtcgtcaatt tatgattaga
    10201 tgaatctgtc acaggaagca caacgacaaa tggtgctgat ttttctttta cttaatctga
    10261 agtctttatt tccgtaaatc ggtaaacagc gtcacgacct tgctcatctt tgtccgatgc
    10321 actaaaagaa gcaatgccct cttcctcgtc tactgttttt gtcacatttt ctgccagtat
    10381 tttttcacta tcactttttt gtgctagttt tctttgtgcg tcttccacac tacttccttc
    10441 tgaccgcagc ttgtaaaatt catccgagac atcatatata gcgaaagtaa cgccgttcaa
    10501 cccttttact ccttgaaagt tatccgggat atttattccg tcgttttgac tgatacttgg
    10561 catcttatcg ctttcgaaca ttcgattatg caaagtaaat gtaataggtg attctctagc
    10621 tgacacagtc ggagaaccta taaaaaagac cagtaggata aaaaagagaa cagcaaatat
    10681 atccacacgt acattttttg tttttttcat cgctgttcac ctgctttttt cttacgatgg
    10741 ataaaataaa agaagccaga aaaaataaag atcagtccta aaaagacaaa gggcagtaga
    10801 cctatcccac ctgtcgaagg aagcaagaga gtcggattga tcgtcaactt agcggtatct
    10861 gttgcgataa tcgtataatt attgtttaca cttccttcgc ctaaactaat cgttactatt
    10921 tgtgatccaa tatctgtccg gctgttaact gttagggtat aatccaatga ttcgcctaag
    10981 acaacaccgg taacgtggag atttccaacc gaaatcgagt gactttgggc atcataatta
    11041 aaggtagcgt catcgacagt aatcactgcg ttagctttat agatggtata agttcctgat
    11101 gtgttggcta tatcagtaaa tgacaacttg ggatttgctg ctttgacttt gtctattccg
    11161 ctattcgtta gtttaattgt atagctgcca gcattaacca tattttttgc tgattgtgca
    11221 tctgctgtgt aatccatact gctaagtgcc acttgtactg gagtaagcga actagtgaca
    11281 ccctctgcta gcttgacatg gattgacggg acacctgctt ttccatcata tgtcttactg
    11341 ccactgagct ctaccgcacc gctgatttgc ttaatcgtat agacatcaga tgtctgcacg
    11401 atagtaaata aatagtttgt tctggcagct agtttagcta gtccgctttt tgtcaattgc
    11461 caagacgtat aggttccaat ttcagtaggc gcagttactt caactccctt ttcattcatc
    11521 gctttgaaat cagttggatt ccatgtgatt tgatcaatac tgagatcatt gtttaaaagg
    11581 gatgttgatt ctgttttatc attaaaggca tatttaacga tcggtacaac agaaagacca
    11641 tcatagtact tactgccatt tgcattgatt gatactgttg catttgtctt ataatatagg
    11701 atgacttcag aaatctgttt tccatttgcg tcctttacga ttacatttcc atttgtatct
    11761 aaactagcta aaacactcat tgctgattgg ttctttgttt ctatcgctga aacagctgta
    11821 aaggtatatc cgttgatagt aggcgtatta gtcgtcaggt cttgtgcagc acttccgtaa
    11881 gtaagattca ttattttaga aggagcaatc atctgtccaa aatagtcttg atacttaata
    11941 gtgattggtg tttgtttgcc tgtataggtc accgtatcga cgttactttc tagggctgtg
    12001 aaagaaccgt ttgtttgtgc aaaagaatag cccgttactg ctgttggagt gataaccact
    12061 ttgtcaccca catggattcc agataatgta gtcccgtacg ttccgatttt tgctgctcca
    12121 ccattgactg tataagaaaa agttcctgtc cgattcgcat tgacctgaac cgttgctgct
    12181 tgataggttg gaaaatttga aacatttccc ttaccatcaa cgatctgccc atttttgatc
    12241 gagtaccccg tgaatggaat atcatttgct aactgtatac cgattgaaaa agtactcata
    12301 cctcctccgg caggaagccc tccccatgtt gagggtgtaa aagaataact tttatccgca
    12361 ttgggtgtaa ctgaatcgat cgtatttcca gcggtagcca cccccatata gatacttttt
    12421 gggaatgtga tagtagtacc atttactgtg gagccaataa acagattatg gatatctgga
    12481 cggttatcaa taaaagcgat tgctttggtt acgccttgta acgcagtttt atccgcactc
    12541 atcgatccgt taaccgcaac aatgactcca tctttggcat atatcactgt atatccatct
    12601 ggtatggtat gtccgttgaa attcgcagtg gccgcaacag tactaaatgt ctggcttcta
    12661 cctgtttctg aataagcttt ctcccctgct gcttttgctg ctgtgatttc tttttctgtc
    12721 ggatcaatca gttgagtcga ggatgtggtt gaggttgtct cgttgttgct agactgattg
    12781 gtgctttcta tggactgtat ttcagaatag ggtggactat ttgtctcctc tgtcgtttgg
    12841 ttagcaaaat ttgacgtttc tgtataaacg tttacataaa tacttgataa gctccctaag
    12901 aaagtaaaaa tcaagatgat cgaaatcata atgggatgta agtattttct tttagataca
    12961 gctttgagtc tagaaacaaa atgtttttga catatacgtt ggattttatt ttgagttttc
    13021 ttaatcattt tcatccctcc tctcaataaa aaatcatcaa ataatccttt acacgttatt
    13081 ctaaatttaa tttttattat tttattgatg tatagaaagt ttggggcttt ttttctcttt
    13141 ttattctaag gttattttag tcttgtcctt ta
//
LOCUS       DAZKKW010000075        13085 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.077, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000075 DAZKKW010000000
VERSION     DAZKKW010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13085
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.077"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(79..2199)
                     /locus_tag="ACG8PO_002084"
     CDS             complement(79..2199)
                     /locus_tag="ACG8PO_002084"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFQ0207383.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLAKGKNVIYIHLESFQQFLIDYKLTDENG
                     LQLEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYTSAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHKG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            2429..3607
                     /locus_tag="ACG8PO_002085"
     CDS             2429..3607
                     /locus_tag="ACG8PO_002085"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HFQ0207384.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIVGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            3623..4381
                     /gene="aroD"
                     /locus_tag="ACG8PO_002086"
     CDS             3623..4381
                     /gene="aroD"
                     /locus_tag="ACG8PO_002086"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFQ0207385.1"
                     /translation="MVWVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLAGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            4404..4889
                     /gene="ybaK"
                     /locus_tag="ACG8PO_002087"
     CDS             4404..4889
                     /gene="ybaK"
                     /locus_tag="ACG8PO_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HFQ0207386.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSHIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(4960..6213)
                     /locus_tag="ACG8PO_002088"
     CDS             complement(4960..6213)
                     /locus_tag="ACG8PO_002088"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFQ0207387.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            6329..7678
                     /gene="gdhA"
                     /locus_tag="ACG8PO_002089"
     CDS             6329..7678
                     /gene="gdhA"
                     /locus_tag="ACG8PO_002089"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HFQ0207388.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNAQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            7790..9136
                     /locus_tag="ACG8PO_002090"
     CDS             7790..9136
                     /locus_tag="ACG8PO_002090"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFQ0207389.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(9444..9830)
                     /locus_tag="ACG8PO_002091"
     CDS             complement(9444..9830)
                     /locus_tag="ACG8PO_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFQ0207390.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYTEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
     gene            complement(10098..10247)
                     /locus_tag="ACG8PO_002092"
     CDS             complement(10098..10247)
                     /locus_tag="ACG8PO_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0207391.1"
                     /translation="MTFTKTNMQYRVAFDTTSNRFMAIDAQNENNVAFGITIEQAVRA
                     LNESR"
     gene            10709..10921
                     /locus_tag="ACG8PO_002093"
     CDS             10709..10921
                     /locus_tag="ACG8PO_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0207392.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPTELVS"
     gene            complement(11199..12998)
                     /locus_tag="ACG8PO_002094"
     CDS             complement(11199..12998)
                     /locus_tag="ACG8PO_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase
                     membrane domain-containing protein"
                     /protein_id="HFQ0207393.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRSFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
BASE COUNT         4291 a         2353 c         2577 g         3864 t
ORIGIN      
        1 ttattctaag atttctcttt accaacatca tttgacaaag agcctaattt tgtcgctgct
       61 ccttttaaat ttattttatc attcggaaga acttgtggtc gttccgtgaa gttcttggta
      121 cgtttgcgtt ttataaagat caacagttga ttgtcccttg tgttcactaa agacactagt
      181 tgacttgtca ccaagagatt tttcagcttc ttttaatttc tctaagccat tcttgtagtc
      241 ataatcactg gtatcaattg ggtctaatcc actattcgta tagaaccgaa gaagatcacc
      301 attattgatt tggtcagaaa cagctaactg tttatcaact ttttctttca gtgaatcaat
      361 ttcattctgt acttcctcag taggttcagt aatagcttca ccagtttcgt tactatagat
      421 cgtaccaccg tagtaagtat attttggtgt aacaaaatca ccatcacgga aagaaacaag
      481 ctgttcatga tctttcgaga atagatcttg tcctaactga atataatttt ttgtatctac
      541 acctagtaag tgaagtaatg taggcaaggc atcaacttgt ccgccgtaag tgtgattgat
      601 tccgccctgt gtttgtccag ggatatggat catataaggg acacgctgca tttgtgcatt
      661 gtcgtagttt gtccaagtag aacttgtttt tccaactaat tcagctagat ttttatttct
      721 tgaattcgag acaccgtaat ggtcaccgta aaggacgatg actgaattat catacaagcc
      781 gctttctttc aaataattga agaattcttg tacggcggta tctaaataat tggctgtcgc
      841 aaagtaaccg ttgatcgttt catcatttgt attggctaac gggaatcctg cttcgtcatt
      901 ggtgaatttt gaataaggat aatggttaga tacagcaatg aattttgaat aaaatggttg
      961 ttgcagacgt tccaagtatt gtacggattg ttggaaaaat ggtttatcat gcaacccgta
     1021 ttggaacgaa ttgtcatcat taacatcata ataagaagaa tcaaagaaat aatcatatcc
     1081 taaattcttg tatgtctcgt ttcgattcca gaatgtacca gcgttcccat ggaataccgc
     1141 actagtgtaa ccttgtgttt gacttaaaat atcaggtgca gcttggaaag tattttttcc
     1201 gcctaattgt gtgaacaagg agccttgatt caagccaaac aatgaatttt ccatcagcgt
     1261 ttccgcatcg cttgtttttc ctgcttttac ttgatggaaa aagttgtcaa aactgaacgt
     1321 gctgttgctg tggtaaagac tattgatgaa aggtgtaact tcaagctgga gaccattctc
     1381 atctgtcaat ttataatcga tcaggaattg ctggaagctt tctaaatgga tataaatcac
     1441 attttttcct ttagcgagcc cgaacaaatc atcatttggt gctgcataat gttcttttac
     1501 ataattttct acttctttca tatcattggg acttgcttct gcccgcactt ggtttgtctg
     1561 gtaagtttga acaccgtcat aaaccatgaa agcattcaag ccaagatatt tgaccaaata
     1621 atcacgagag aatgttcgag tcagcaattc tggacgatca gcttccgcca taaaaagatt
     1681 tcctgagaaa atcataactg ctaatgtaga gatcgcaaaa gccattcttg ctcggatcgg
     1741 gcgagggtcc aacttgattt tttttgcaat cagcagaaca gtcaagataa taaaatcgat
     1801 ccaatagatc acatcatatg ggcgaaacag tcgaatggca ctttctccca gaccactcgc
     1861 tactttccca gcacctaaaa tcgtgttgat cgtaatgaag tccgtgaatt ctcgataata
     1921 agtgacattc gaaaaaacaa gcaatgtcaa aagaaaataa atgaccatca tcgtgatata
     1981 agcaactcta gggcgtctga tataaagagc aattccaagt aataacaatg tagttgcaat
     2041 agggttgata aacaagataa agtattgaat gccactttcg attccgagat ggaaatcaac
     2101 taaataggca aaaatcgttt tcaaccaaag taaaacaact agtaaagaaa aaaatccgag
     2161 cctcttattc aaaaaggcag gtgtttttat acatttcaag ataatcttcc tttctgcact
     2221 tcttaatcac agaagtgtca tgaaaccatg ttacacagct cacttattct catttatttt
     2281 actagtatga caatatagtg tcaattgaaa ccagaagttt atgctgtttc attaaggttt
     2341 tctttaagct tcatgaaaaa atgagcagta agacaagtcc tatgtttttt gttatacttg
     2401 ggaagagaaa gctaaagcag gtgaaggaat gaaagtaaaa gtaaaaaaaa gagcaagcaa
     2461 tcgattgaaa aaaggatatc cactgatcca aaaagaagac cttgctcatc cccaaaaaga
     2521 aaccacgcaa tgggtggaat tagttgattc gcaaaaccaa tttatcgcaa aaggatatct
     2581 aggaaagcag aataagggaa tcggttgggt attgagccag cagaacatcc catttgatca
     2641 atcttttttt gaagaatttt ttgtgaagtc aaaagaaaaa agaactttct ttttttcaga
     2701 tgattctact accgcttttc gtatattcaa tggagaagga gacgggatcg gcggtctgat
     2761 tgttgattat tatgcaggtt atgctgtttt ttcttggtat aacgaaacgt tatatgcaca
     2821 tcgtgaatta ttgattcaag cttttcgtga aatctacccg gaaatcgtag gaggctatga
     2881 gaaaattcga tttgaatcag taaatctgcc tgagagccag ttcatttacg gaaaagaggc
     2941 accggaaccc ttgatcgtaa aagaaaacgg agtaaaattt gccgtttatt taaatgaagg
     3001 attaatgact gggatctttt tagaccaaaa agaagtacgg ggatcattag cagaaggttt
     3061 cgcttctggg aaaacagttt tgaacatgtt tagttatacc ggcgcctttt ctgttgctgc
     3121 cgcaatggga ggggcaatcg aaacaacaag tgtcgatcta gcaaaacgca gtttaaaaaa
     3181 aacacaggaa cagtttgaag tgaatggttt ggatgtcgat cagcaaaaaa tcattgttat
     3241 ggacgtattc gagtatttca aatacgccaa aagaaaacaa tttttatatg acatgattgt
     3301 attggatcca ccaagttttg ccagaaataa gaaaaaagtc tttcgcgtag caaaagatta
     3361 tggggaactg gtcaaggata gtctggatat tttagcagaa gacgggatac ttattgcgtc
     3421 tacaaatgct gcgaatgtct cgatagaaca attccaaaca atgattgaag aggaattcca
     3481 tgccaaaaat gtttcttttg aacaacttgc tttatatcgt ctgccaaaag atttccagac
     3541 gacggatacc tttttagaag gcaattatct gaaagtgttt gtttatcaaa ttaaaaataa
     3601 ccattagaag gagcgatgca gaatggtttg ggtaaaggga ttgaatatag gaagcggtag
     3661 accaaagatt tgtgcgcctc ttatcggggc cgtccgagag gaagtattac gtgaagcagt
     3721 tcttgcacgt gaagcagggg ccgatttagt cgaatggcga gtcgatcatt atcgtgaagt
     3781 tttttctttt gatgaagtag cagaaacatt ggttcttatc catgagatgc ttgatggact
     3841 gccgttatta tttacgtttc gtaccttagc tgaaggcgga gaacaagaca tatcgatccg
     3901 tcagtatcgc gacctttatg aacatgtgat acacacagga ttagtagata tggtcgatat
     3961 cgagttgttc aaagtagaaa gtctaggaaa aagactgata gaggaaatca agtctttaaa
     4021 cattccttta attatcagta gtcatgattt taaagaaaca ccagcagacc ctgtcttgct
     4081 ttaccgattg aatgttatgg aacattttgg agccgatatc ggaaaactgg cagtaactcc
     4141 caataatgaa cgagatgttt tgcggttgat ggagttgact agacgggcaa atgcttttgt
     4201 ctccatgcct attattacga tgtccatggg aaatcttggt aaaatttctc gtctggctgg
     4261 cggaacgacg ggttccgtta tgacatttgg ttccttaagc agcgattcta cttctgctcc
     4321 aggacaatta ccggtcaaag cattgaagca aattattcaa ttattaaatg aaccgttgta
     4381 gaacaagttt gaggtgatat tttttggcaa agaaaaaaat agtaaaaacg aatgcaatcc
     4441 gtatggtcga acaaaaaaag attccctata cagagcacga atatgagtgg gacgaaagcc
     4501 acctaagtgc gtcatcggtc gctgaacagc ttccagaaag ccaatctcat atatttaaaa
     4561 cactggttgc tgtgggcaat gtgacggggc ctcttgttgc tgtgataccg ggagaagccg
     4621 aattgaatct aaaaaaatta gcaaaagttt cagggaataa aaaagtagaa atgctccatt
     4681 taaaagattt ggaagcaacg accggataca tacgtggtgg ctgctcgcct ataggtatga
     4741 aaaaactatt tcctacttat ttagatcaaa ttgcagaaag ctatgaacaa atcattgtct
     4801 cggctggcag aagagggcta cagatggagt tagcacctca agatatctgt gcattaacga
     4861 gcggacaatt tgcagatata aaacaataaa tcatacgtat aaaagaaaga aggaggccgg
     4921 gatgttgttt gtccagtctc cttctttctt ttttatacat tatgctcttt gcccggcatt
     4981 cattcctgct acatgccctg tagcgaaagc cgctgtgata ttaaagccgc cagtataacc
     5041 attgacatcc agtaattctc ccgcaaaata cagaccatca atgattttac tctccaatgt
     5101 tttaggataa atttccttta gagaaacccc tccgccagtt acaaatgatt tctcaatagg
     5161 aaaagttttg ttgattgata gcttaaactc tttgcatagt tgaacaaaat cttgaatttg
     5221 tttttgagag gcttgttgtc ctgtcagatg atccatttca agccgctcaa gaaagaaaac
     5281 taacaatcgc tcaggcaata taccatgcca agcattgacc aagttctttt tggttgcttt
     5341 acttttttct gtcaatatat gaataagctc ttgtttggtt tgtgtaggga aacaatccag
     5401 agaaagtgtc actggttctc ccgttttctc taattcttta ttgacaaaac ttgaacaacg
     5461 cagtgcagcc ggaccagaaa taccgaaatg ggtgaaaagt aaatccatcg tatgttcagt
     5521 caatacacga ccttttttat ttaaaacgcg taaagtgatg tcttgtaaag aaagcccttg
     5581 aagtgttttc tcctgaatat acggttcatc cgatatcaag ggggactctg tcgcataaag
     5641 aggcgtcacc gtgtgaccca ctcgcttcac gatcttgtac ccatctccag tagaaccagt
     5701 agatggataa gtccgtccac cagttgttaa gataatggca ggagcatgga actcttcgaa
     5761 ttccgtgcgt ataccgtaaa ttttatgttc tttgtggatc aatttctcta ctcgagtaga
     5821 gaatagaagc gtcacatcta gttctttcag acgattgagc aatgcttcga taatcgtttt
     5881 tgatttattt gttacaggaa acatccgtcc atgatcctct tcttttaaat gtacgccttg
     5941 tgattcaaag aagttcatga tatcgaaatt attccattgc gcaaacgtac tatataaaaa
     6001 ctttccattt ccaggaatat gtgcaatcaa atcatcaaca ggacgattgt tcgtaacatt
     6061 gcaccgacca ccacctgtca tcagtagttt ttttcctgct tttttgtttt tttcgatcaa
     6121 gagtacgcgt gcgccttgtt cagctgcact gatcgctgcc atcatcccgc ttgttccagc
     6181 gccgactacg atcacatcat acagtttttc cattccctca cctcaaggaa agtgtagcat
     6241 atctgaagaa agaaaacaga aaaaatgttt tgagccaaaa gagaaaatga gctaaaatga
     6301 gggaagataa gcaaaggagc gtggaacgat gacaaacgca acagaatatg ttcaagcaat
     6361 tcaagaaaag ctacatcaaa aggatcaagg gcaagaagaa tttttacaag caatcgatga
     6421 attcatgccc acggttatgt cttttttgga cactcatccc gaatatatcg aaaagaatat
     6481 cttagaactt ttaacggaac cagaaagagt tattcaattt cgagtgcctt ggcaagatga
     6541 tgaaggaaac tggcgagtaa atagagggta ccgtatccaa tacaactcag ctatcggccc
     6601 ttataaaggt ggattgcgct tccatcctag tgtcaatcta agtatcttga aatttttggc
     6661 ttttgaacag attttcaaga atagtttaac cggtctgcca atcggtggag gaaaaggcgg
     6721 aagtgacttc gatcctaaag gaaaatctga taatgagatc atgcgttttt gtcaaagttt
     6781 catgctggaa ttgtcaaaac atatcggccc atcgattgat gttcctgcgg gagatatcgg
     6841 tgtaggagct agagaaatcg gctatttgtt cggcgaatat aaacgattga aaaaatatga
     6901 tactggtgtg ttgacaggta aacctttgga tttttgggga agtaagatac gtacggaagc
     6961 tactggttat ggattagtgt attatgtcaa acatctattg aacgaagaaa aagattcatt
     7021 taatcaaaaa acagtttttg tttccggaag cgggaatgta gccatttatg caatcgaaaa
     7081 agtgcaagag ctaggcggga aagttgttac atgttcagat tctagcgggt acatctatga
     7141 tccagaagga atagatgtga aactattgaa agaagtcaaa gaaatcaata gacaacgttt
     7201 gacggaatac gcaaaccaac gtccttctgc tatttatcat gctggagaat ctgtttggac
     7261 attgaaagaa aaagcggata tcgctttgcc atgtgctact caaaatgaaa tagacgaaag
     7321 cttggccgaa atcttagtag aaaatgggat caaaatcgta gcagaaggcg ctaatatgcc
     7381 atgtacagta aaagctgttt ctgttcttca tgaagcaggc gtgtggtatt gtccaggaaa
     7441 agctgctaat gctggaggag tagcagtatc tgctttagaa atgagtcaga acgcccagcg
     7501 attgacttgg gaaagagagc aagtggatga gcagctggat caaatcatgg aaacgattta
     7561 ccatacgtgt cgccaaacag cagccgagta cggcgacgaa aaaaatctgc ttcttggagc
     7621 aaatgttgct ggatttgaaa aagttgccaa agcaatggca gcagaaggtt tagtttgaaa
     7681 aatgaaactt tcttggaaat attgtcagca ttttgtttat ttgcttgaaa aataataaaa
     7741 aaaaacgtaa agtattacct gtagagtaaa cctataagga ggagaacaaa tggcacacat
     7801 tcattttgac tattcaaaag tagcaccgtt tgtcaacgaa caagagttag ggtacatgca
     7861 aagccaagta acagcagccc acaacgaatt acgggaaggt actggcgcag gaagcgactt
     7921 ccgcggctgg atcgatttac caacgaacta tgacaaagaa gaatttgcac gtatcaaaga
     7981 agcagctaaa aaaatccaat ctgattctga agtattagtt gttatcggta tcggtggttc
     8041 ttatttaggc gctcgtgcag cgatcgactt cttgaatcac acattcttca atttattaga
     8101 tagcaacaca agaaaagcac cacaaatctt ctttgccgga aattcaatca gctcaacata
     8161 tattgctgac ttgatcgaag taatcggtga tcgcgacttt tcagtaaacg ttatttcaaa
     8221 atctggaaca actactgaac cagcgattgc gttccgtgta ttcaaagagc ttcttgttaa
     8281 gaaatatgga caagaagaag caaacaaacg tatctacgct acaacagata aagcaaaagg
     8341 tgcagttaaa gtagaagcag atgcagaagg ttgggaaact ttcgtgattc ctgatgacat
     8401 cggcggacgt ttctctgtat taactgctgt tggtttgtta ccaatcgctg ttagtggtgc
     8461 agatatcgat gcattgatgc aaggtgccgc tgatgcaagc aaagcatact caagcgacaa
     8521 actagaagaa aacgaagctt accaatacgc agcaatgcgc aacattcttt accgtaaagg
     8581 caaagtaact gagttgttga tcaactatga accaggaatg caatacttct cagaatggtg
     8641 gaaacaattg tatggtgaat ctgaaggaaa agaccaaaaa ggcatctatc cttcaagcgc
     8701 aaacttctca actgatttgc actctttagg acaattcatc caagaaggac gtcgcaatat
     8761 ttttgaaaca gttgtgaagg ttgaaaaacc tcgtaaatca atcacaattc ctgaacaagc
     8821 agaagattta gacggtctag gctacttgca aggaaaagaa gtggacttcg taaatacaaa
     8881 agcatttgaa ggaacattgc ttgctcatac agacggtgac gtgcctaact tgctagtcaa
     8941 aattccagaa atggatgctt atacattggg ttatacaatg tacttctttg aaattgctgt
     9001 aggtatttca ggttacttga atggaatcaa cccattcgac caaccaggtg tagaagctta
     9061 caagaaaaac atgttcgcac ttctaggcaa accgggcttt gaagatcttg ctaaagaatt
     9121 gaacgaacgc ctgtaagata agggtttttc gctagattta aataatcagt gatagaaaca
     9181 tgttcgcttt aaaatgaaaa ctggtaccca tttaggtact caaacatact gcatcgttta
     9241 ttcagaataa ggggctgtga caagacgttt gtcacagtcc cttgtttgta tagaaatcga
     9301 ttgattgcct tgctctttag tttgttttag gactttaaga acagtgaggc tgacttcgaa
     9361 ggttaattct gtttttttgt tttctagaat cagtaagcaa aaaagcactc acttcgagtg
     9421 cttttttgcg atatattctt agcttaagat aggcctaatt tttcttttac agatgagggt
     9481 accttatcgc tggtaattac ttttttctct atgatccgct tttcgcttgc tttcaactgc
     9541 aagatcgttc cttcggtgta agcagaacca ttggcagagt caccagaagt aatgacatcg
     9601 aaatctaact gtcttttttc accgttttca gcataagcat ctacgttgta tttaaaacct
     9661 cttccaatta cttccccatt atctgctttg atatcagttt ctgcaggcat tggagctttg
     9721 actaccgcat aataatcttt acctacataa gtagactgat agtagtccca acctttccat
     9781 cctataaaag caatcaatat gataacgatc aaccctaaaa tttttttcat gaaagatctc
     9841 cttttttatt tttattatat taaatattat acagataata acggaatatt atcatcgaaa
     9901 cagctaacaa aattgtcata aaaaacgaga tggttgatat tagaacttgt gtttttgttt
     9961 aggtctagag ataaagagag acaatttcca aatatatgta atgaatacaa aaagacgttt
    10021 gtcgcggatt accgttacaa acgtctttta ttgattttcc gagatctcat cagtcgccat
    10081 ttggaatggt tcactcctta tctagattcg tttaatgctc ggactgcttg ttcgattgta
    10141 ataccaaacg caacgttatt ttcgttttgt gcatcaatcg ccataaaacg gttagaagtt
    10201 gtatcaaaag ctacgcggta ttgcatattt gtttttgtaa acgtcataaa attcatctcc
    10261 tttgtaatgt aatttagttc ttgaaagttt cctttaaact ttcgaactta taccaagttt
    10321 agcatcgtac gttttttgat gcaattaaaa agcatttatc attttcctta tttatttttt
    10381 cttaaatggt ttagctattt tcttttaatc cgataagtag cccaatcatc aaggttacaa
    10441 atactattgc ttgaatataa atcatatgct cacctcttta taaataactt agcaatataa
    10501 agaaaaaaac gcacgaacaa cataggaata taaacaaacg tttttgtcac aacgtctaag
    10561 atcgtgttaa aaaatagtta tctaaaaagc agaaagatac tcaaaataaa aaataaaaaa
    10621 atgaataaaa acgttttcaa atacaaggta atttgttatt ataaaataaa gaaaaaacaa
    10681 aataaaaata aagaaaagag ggaatgaaat ggtaaaagca gaagtaggaa gatcttatat
    10741 atgcaaaccg atcggagtat ctcatgaact aatcggctgt attgaacgta catatatcaa
    10801 cacggctgtc gtgtatgttg aaagttatga ccaacgagac tatcaactta tcaatgaaag
    10861 caagtatcgc atgctcgtaa aattctgtga tattagcgca cctactgaat tggtttctta
    10921 aacagtattt ttgagaattc gcaagaactc ccttataata taaataaaga cagaagggag
    10981 cgagaagata tgccaaaaag aaaaagttca gggaaaatgg gaattattct tgcagtgatc
    11041 tctgcaattc ctgctatttt agctattgtt cgtgcgatga aggaatcaag agaaagttaa
    11101 gcacacatta tgagcgtcaa acaaaaaacg catatacctg cggtggcagg ttatgcgttt
    11161 tttgttgtat aaagactttt ttcattaacg atttttgatt agccgactcc caaaacaaaa
    11221 tttagcagtt tatctgaata agtgatttct tcatctgttt gtttcacact ttcatttagt
    11281 agtcccatct catcggttat gataccatcc acaccataaa acatcatgcg actcatggca
    11341 tctgaatcgt tgattgtcca tacatatact tcttttccgt cttggtgggc agaattaatg
    11401 aagttacggt tgatcgtact atattccatt gtcagaaaat cagcaggcgt cacagggggc
    11461 ccaacaatat tgaaaggtaa gatgtagccc acataaaagt ttggttctgc ttcttttaaa
    11521 tctgaaacgg tttggtacgt caaactttgc aaaatatgat ggttctctaa gataactttg
    11581 cggtatttat ggacaaatcg ttcgacaagg tcatccgaat cttttttaga ggtcttgatc
    11641 tcaataagta atttttggtt tagcttatgt gctttttcca aataagaatc aaaagaacaa
    11701 acaggggctt gcactccgtt ttcagtaact gtaagatttg tcaattcttt caatgtaaga
    11761 tcctgtggtg ttccatttac ccctgttaag tgacgaagat ttgcatcatg catcactacg
    11821 aaatgaccat cttttgtttc ctgaacatcc atctctacat aatcaggatg atagtctctg
    11881 cttgtttttt caagtgcttc taaagtattt tgcgccccat tttgggtact cacacctctg
    11941 tgagagacag tcactggttt tgtctgaaca gctgaagtca gataatccat attgtaaaga
    12001 cagacgccaa taccgaaaaa aactgcaaaa agagtcaaac cagtgttttt cagcgcagag
    12061 aaacgcaagt tgggggcttg tgggataaac cacttaggga tctcaggcaa gaagccttcg
    12121 tcatccatga aatcaacaat gatataaaaa atccctacgg tagacatcac gatattgaat
    12181 aataaaatcc cttgtaaaag cgtcatagca aacacagcgc tgatcaatgc atagtctggg
    12241 aaaaattgtt caaccatact ttgtgccaaa ataacaacga tatatcctaa agaagaaagg
    12301 agtaaaatcg taccgccaat cacaataaat tggcccgtga tggccaaaag acgcgatttg
    12361 gtcaatgacc agctttcccg aatggctgct cgaaaagaac gatctcgtag aatcatttcc
    12421 ggtaaagcaa aaatcagtcg aatcccaata tagccaagga aaatataaac gagtaaaaag
    12481 gaactgacga tgatccaacg attagcaaaa ataaaatcca taatgaaagc ggggattttt
    12541 atttttgaca gtagatcaga attaaaactt aatccgctga tcggtagaat caataaaaaa
    12601 taagcaagga aaaacaaaaa agtaatgggt ctcactttct tcagctgaag tatcgtcaag
    12661 tgtaagagtt gcttcaaaga gattggttct ttctttttaa taaaaaaaac actcatcaaa
    12721 agaaaagtaa attcgaaata aactaacagt aaaatcgtca tcaaaactac gattaacgag
    12781 agtagaaccc caggatgttg agaaaaaatt gaaggaatat tgtcataaga caaatagtca
    12841 attgcccctc ggtgtaaaat aaaacgggtc gtactgctca ataagggtaa acaaataaat
    12901 aaaataaagc catgcatcag caagacatca cggaaataag cttgtgttcc ttttaaaaag
    12961 tcccaagcat tacctaagct acgtttcaaa taattcatcc tatcacctgc tcttcacatt
    13021 gtagcgcaaa tctaatgaaa aaggaaaggg tgcagtgtgt tttgcagacc ctttcccaga
    13081 tttaa
//
LOCUS       DAZKKW010000076        12515 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.078, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000076 DAZKKW010000000
VERSION     DAZKKW010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12515
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.078"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     regulatory      198..364
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
     gene            512..1069
                     /locus_tag="ACG8PO_002095"
                     /pseudo
     CDS             512..1069
                     /locus_tag="ACG8PO_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
     gene            1127..3166
                     /locus_tag="ACG8PO_002096"
     CDS             1127..3166
                     /locus_tag="ACG8PO_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFQ0207394.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKIAFDSLGGKAASTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKMITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSFKTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(3275..4426)
                     /locus_tag="ACG8PO_002097"
     CDS             complement(3275..4426)
                     /locus_tag="ACG8PO_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFQ0207395.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(4580..5110)
                     /locus_tag="ACG8PO_002098"
     CDS             complement(4580..5110)
                     /locus_tag="ACG8PO_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HFQ0207396.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(5209..5559)
                     /locus_tag="ACG8PO_002099"
     CDS             complement(5209..5559)
                     /locus_tag="ACG8PO_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HFQ0207397.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(5716..7245)
                     /gene="ahpF"
                     /locus_tag="ACG8PO_002100"
     CDS             complement(5716..7245)
                     /gene="ahpF"
                     /locus_tag="ACG8PO_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008785 - alkyl hydroperoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HFQ0207398.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     RTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(7255..7818)
                     /gene="ahpC"
                     /locus_tag="ACG8PO_002101"
     CDS             complement(7255..7818)
                     /gene="ahpC"
                     /locus_tag="ACG8PO_002101"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HFQ0207399.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(7962..8624)
                     /locus_tag="ACG8PO_002102"
     CDS             complement(7962..8624)
                     /locus_tag="ACG8PO_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0207400.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            8808..10454
                     /locus_tag="ACG8PO_002103"
     CDS             8808..10454
                     /locus_tag="ACG8PO_002103"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFQ0207401.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAVLLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPNMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDLQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            10445..11527
                     /gene="uxuA"
                     /locus_tag="ACG8PO_002104"
     CDS             10445..11527
                     /gene="uxuA"
                     /locus_tag="ACG8PO_002104"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFQ0207402.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFGLPGWEP
                     ERLAPIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLA
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(11635..>12515)
                     /locus_tag="ACG8PO_002105"
     CDS             complement(11635..>12515)
                     /locus_tag="ACG8PO_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HFQ0207403.1"
                     /translation="KENFPATSVKDVQKEFTEYEYIDFFIDLSVDILRPNLPICREQR
                     ILVNILARLSITKIESEETSLEQYKLLKKANVPHVLYSREMVESLCTVFDLFIPEKEK
                     ILYSLAFARNKLYNGFLNFNQPNTPLPTFMLYQEHSVLKEVQTAIEQFYMDFREQNQH
                     LLPVILEKENVQWMVEDLVHLYDRYKKQPRIVIGVNYTRDFYISKDLIVKIEQIFSNE
                     SIKIQNDFMENCNIVISDCPLKQLPSHIKKIYILKGIITPEDWKKVIMRISEYIFELK
                     EEALMDQSKKENQVTS"
BASE COUNT         4184 a         2307 c         2341 g         3683 t
ORIGIN      
        1 tataaaaaaa cacataaaac taaaaaatta tcaaagaatc ctggtgcaac tgaaagaatt
       61 tgcaacggga ttctttcaac ttatttgtta tttttttcta aaaaatatta aagattgtta
      121 aatttcacta tttcatttca aattccattg cctaatcatt aggaaaagtc tatcatgaac
      181 tcgtaaatag aatatagtaa taaccttgtt aggtgaggct cctatacaaa cataggctgt
      241 tgcccagaaa cgtcgaaagt cgccaatggg taaaacagaa actgttggat taaggctttt
      301 tccaatagct aagaatgttt tctttacgtt gtatagtgct aaaactcgga cgatgagatg
      361 atgctaggtc agaattcttg ttcgaaaagg tttctgacct tttctttata tttttgtgac
      421 tgacttattt gatgagacaa actcgctcct cacctgacaa gtggattgct gtgtgaggag
      481 gagatgcggt gaaaaaagat cgattggtgg aggtcgctcg gattcacttc tggttgcaca
      541 atacaaccag aaaacacaac aagcaaaact gatctctatc atgagagatt catatgtcga
      601 tattccaggt tacggaatga ataagataaa tgcagcttac tcttatgggg gagttgattt
      661 attaaatcaa acattaaagg aaaatttcaa atttgaagct ccctattatg caagtatcac
      721 atttcaagat tttatcgatt gcgttaatga actgtttcct ggtggagtca agatcgatgc
      781 agaaaaatct ctagatttag atggcgtata tataaacaaa ggagagcaag tgatggatgg
      841 taatacttta ctccagtatg ctcgatttcg ggaagacaaa gaaggagact tcgggaggat
      901 cagaaggcag cagcaagtga tcaaagctat ctctcagcag ctgaaagacg taacttcaat
      961 attcaagcta cccaaagcgg ttgggaaatt acttggtagt atccaaacga accttccaga
     1021 aagtgtgctt ctggattgtg gtatggattt cctcaaagac atatgttaga ataaacaggt
     1081 ataaatagtg aaataaagga atgacaagca agagaaggag gaaaaaatga aaagaagtga
     1141 caagcacggc aaaaatcgaa caggcgctta tattgccggc gcagtgatct taatagcagc
     1201 tgcgggtggc ggttattttt actaccagca ctatcaagaa acccaagcag tagaagctgg
     1261 agaaaagaca gttgagcaat ttgtccaagc tttgaacaag ggagattata acaaagctgc
     1321 agaaatgaca tcaaaaaagg cggcaaataa aagtgcatta tctgaaaaag agatcttaga
     1381 taaataccag aatatatacg gtgctgctga tgtcaaagga cttcagatat cgaatctaaa
     1441 agtagataaa aaagacgatt ctacttacag tttttcatat aaagcaaaga tgaatacctc
     1501 attaggtgaa ttgaaagatc tttcttataa aggaacatta gacagaaatg atggtcaaac
     1561 cacgatcaac tggcaaccca acttggtttt tccagaaatg gaaggaaatg acaaagtaag
     1621 cctgaccaca caagaagcag caagagggaa tattatagat cgaaatggtg aaccattagc
     1681 aacaacaggc aaactaaaac aattaggagt cgttccaagt aaacttggag atggaggcga
     1741 aaaaacagcc aatatcaaag cgattgcttc ctccttcgac ttaacagaag atgctatcaa
     1801 tcaggcgatt tcacaaagct gggtacaacc cgattacttt gtcccattga aaatcattga
     1861 tggagcaacg ccagaacttc cagctggagc taccattcaa gaagtagacg gcagatatta
     1921 tcctttgggt gaagcagctg ctcagctgat tggttacgtg ggagatatca cagcagaaga
     1981 tattgataag aatccagaat taagcagtaa tggaaaaatc ggtcgctcag gtttggagat
     2041 ggcttttgat aaggatcttc gtgggacaac aggtgggaaa ttaagcatta cagatgcaga
     2101 cggtgtagag aaaaaagttc tgatcgaaca tgaagttcaa aatggcaaag atatcaaatt
     2161 gacaatcgat gcgaaggcac aaaaaatagc ttttgacagt ctaggaggaa aagctgcatc
     2221 aactgttgcg acaacgccaa aaaccggtga tcttcttgcg cttgctagct ctccaagcta
     2281 tgatccaaac aaaatgacaa acgggatctc acaagaagat tacaaagctt atgaagaaaa
     2341 tcctgaacat ccattcatca gccgatttgc gacaggttat gctcctggat ctacgtttaa
     2401 aatgattaca gcagcaatcg gtctcgacaa cggcactatc gatccaaatg aagtgttgac
     2461 gatcaacggg cttaaatggc aaaaagacag ttcttgggga tcttttaaaa caacgcgtgt
     2521 cagtagtgat gtatcacaag tagacttaaa aactgctttg atctattccg ataatatata
     2581 tgcggcacaa gaaacgttga aaatgggtga gaaaaaattt cgtacaggct tagataaatt
     2641 catttttggt gaagaccttg atttgccaat tagtatgaat ccagcacaaa tttctaatga
     2701 agatagcttt aactcagata tcttgctagc tgatactgga tatggacagg gcgaacttct
     2761 aattactcct atccagcaag cagcaatgta ttctgttttt accaacaatg gcacacttgt
     2821 ctatcctaaa ttgattgcag ataaagagac aaaagataag aagaatgtca tcagcgaaac
     2881 agcagtacaa acgatcgtgc cagatctgag agaagttgtg caagatgtaa atggtacagc
     2941 acattctctt tctgctttag ggattccgtt ggcagcgaaa actggtacag cggaaatcaa
     3001 agaaaaacag gatgtaaagg ggcaagagaa cagtttcttg tttgctttca atcttgataa
     3061 ccaaggttat atgatggtca gcatgttgga aaataaagaa gatgatgatt cagcaaccaa
     3121 acgagcatcg gaactattgc aatacctcaa ccaaaattat caataaaaaa ttgcttttta
     3181 agtacagtaa aaggactgtg gcgttatcgt cacagtcctt ttttcaaata aacggttttc
     3241 acgatgtctc tttcacaact tctttttgat ttactcacac ttcataaact ttttttgtaa
     3301 aatatttgag aacaagtgga gaaataagtg tacttaataa aatcacgata actaaaggtg
     3361 aatagtactg tggttcgatc aattgacttt gctgtccaat ttgtaaaatg atcaaagcca
     3421 tttctccacg cgaaatcata ccagcaccga ccatcaatga actatgttga gaaaaaccgg
     3481 caatctgagc accaaaataa cctccaagca atttggtcaa aatcgctaca agcgtcaata
     3541 tagaaataaa gagtagttgt tcactaaagt cagaaaaatc aacttctaat cctacactga
     3601 caaaaaatac tggtataaat actgcatatc ctaaagcctc tacattgtgg tatacttctt
     3661 ctttaacctt tgtctgactg acagctatcc cagcaaaaaa agcaccaatg acagagctaa
     3721 gcccaacaag atccgctacg taagacattc ctaaacaaat aactagcgat acgatgataa
     3781 tagaagaatt tgcgtacact ttttctgcaa atgaaagtag gaaaggcgca atccatttga
     3841 ctaataagaa aataaaaaca aaataaatta gctgttcaat caaaacaagc ggtaaagaag
     3901 cattgctaga agcttccccc gttagaaaag ataggctgaa acttagaact aaaacgacta
     3961 aaatgtcatc taccacagaa gcacccaaaa ttgttgatcc ttcttttgta ttcactacgt
     4021 ttagttcttt taatacttct actgaaatac tgacagaagt tgccgcaaga ataatcccaa
     4081 agaaaatcgc ttcatttccc gaaacttgga atgctttgcc tgtcaaccag ccaaaaacaa
     4141 ccgggaagat gatcccaagc aaagcaacga acataccagg tcggaaatat tttttcaaaa
     4201 gagataaatc actttccaga ccagctaaaa acattaataa gatcacacca atctccgaaa
     4261 agtcatggac tagaatatca ggtttgaccc aattcaatcc agcttggcct agcaaaacac
     4321 caacaagtaa ttgtccaatc actgctggga tacctaatct ccgcgagata tgtgagccaa
     4381 tcgttgttga aatcaatatc aggcataaaa taccaataaa ttccattctc atcattcctt
     4441 tgtataaaat atagcccgaa cttagcataa catacattat tgaaaaaata ggaaaatata
     4501 ctcacaaaaa aaccaacaat ccaaaagatt tttttggatt gttggttttt atacggtcta
     4561 ttttaataac taaaaatgat taacgtatag tcatttttct tattctgatg aaattgataa
     4621 tctcctggat gcccatgaaa atcaaaataa agccgaatat ttggaataga agcatcaaac
     4681 ttttgaatgg attgaacaat aagacgaatc cgccaatcaa caacagaata ctactataaa
     4741 cactccaact tttagctttt gtattcatgc tgaagaacaa ctggaacaag ctgttgatga
     4801 taacagctaa tccaagaaga atcggcagga tagaaacgat tgcaggtgca aaagcataga
     4861 cgatcagagc taagatcagg aaaaaaagac cactgaacag accgatactc caagaacctg
     4921 ttttcctttt aaaacggtaa gcctcaaaaa gattcaacaa cccaagcaaa acaagatagg
     4981 cggtgatcag ataaccgata aaatgaaaaa cagcagaagg gtttagcaca atcgcaatcc
     5041 cagaaataat ataaacagca gctcttaata aggcgtattt ttgaatattt tgtttaatcg
     5101 tctcaaacat ataagaaaac ctcctttgac ttattttaac gtgaactagt cattttacca
     5161 aattaaatgt tacaaaatga gcgaaggaga agaatatctc tggaaaaatc aacttctgag
     5221 gatcttttcc atttttttgc cttttgctag ctcatcgatc attttatcta aataacgaat
     5281 ttctttcatc aagttatcct cgatgtcttc tactcgtaca ccacaaacaa cacctgtaat
     5341 caaggaacgt gaaggattgg ggacaggggc ttccgcaaaa aattgttcga aagtcacttg
     5401 tttttgtagt tgatattcta gttctgtttg ggaataaccc gtcagccaac aaatgatttc
     5461 atctacttcc tcttttatac gatcttttct ctccgctttt gctacataca taggataaac
     5521 gcttgcaaaa cttgtcgtaa aaatacgatt ttgcgccata atcagtcacc tttctttttt
     5581 tcaaattatt tactacttat agtatataag aaaaagagaa gaaaaagcga ttattcaaaa
     5641 aaacaaaaag gattgtgaca aaattcttgt caccatcctt ttttccgctc tctgactttt
     5701 tacaatcttc atagctcaag tgcgcatcag ataatcgaat gctcctaaag ctgcagtagc
     5761 tcctgaaccc attgaaacaa tgatttgttt gtatggacta tctgtacaat caccagctgc
     5821 gaaaacgccg gggatattcg ttgcgccatt tttctcggtc actatttcat ttcttggatt
     5881 catttcgatc gttctatcta gccaatccgt attagggagt aagccgacta gaatgaatac
     5941 tcctgaaaca gctagtgtat gcatctccat cgtacggcga tcgatatagg agagagattc
     6001 tacctgattc gtaccgctga tttctttcgt cgctgcatgt ttgataacag tgacattcgc
     6061 aagagagact agtttatcct gtaagatttg atctgctttt aattctggta agaattctag
     6121 aacatataca tgttttgcta acccagccaa atcgattgct gcttcaacac cagaattacc
     6181 accaccgata acagcaatct cttttccttt aaataaaggg ccgtcacagt gtgggcaata
     6241 agcaattcct ttatttttaa actctttttc gcctggtaca ttgatagaac gccagtgcgc
     6301 acctgtcgcg atgatggcgg tttttgcttt aagtgatata ccgttagcta agccaatatt
     6361 aataaaatgt tgctctttat ttaaggaaac ggcctgttga ttcatcataa tgtcgatagg
     6421 gtaactacgc acatgacttt ctacttgctg catcaatttt ggaccttctg tgtaagaggt
     6481 accaatcata ttttcaatcc ctagtgtttc aacgacttgt ccgccgaaag aatcagcgac
     6541 aagtcctgta cggattcctt tacgtgctgc atagatagct gcactactag ctgctggtcc
     6601 accgccaatc actaatacat cataaggttc tttttcaaac aactcttctt caatcaaagg
     6661 gccagtgatt ttttcaacaa tttgttcgat cgtcatgcgt ccgctactaa attcttctcc
     6721 atttaaaaac acagttggca ctgccatgat ttttttatca tcgatttctt tttggaacat
     6781 cccaccttca atcatcgtat gagtaatatt tggatttaat acagctaata tattcaatgc
     6841 ctgtacaacg tcggggcaat tatgacaagt caagctaaca taactgtcaa aatgcaatgt
     6901 ttgtgttatt tttttgattt taagactcaa agattcactg attttcggtg cccgtccact
     6961 aatttgtaac atagccagta aaaaagagga aaattcatga ccgaggggga taccagcaaa
     7021 aacgattcca ctttctttgc caactggatt gatagaaaaa ctaggtgtgc gcatcgaatc
     7081 tcttttttcc caagagattt taggagacat ttctgctatt tccttcgtaa attcagctag
     7141 tttttgacta ttttcactat tatccaaact aacagaaaaa acaataggag attcaatcag
     7201 tgctaaatat tgttcgagat tttgtttagt ttctttgtct aacatatcca ccacctaaat
     7261 ctttccaaca agatcaaaac ttggtttcaa tgtttcgcca ctttctttcc attttgccgg
     7321 acaaacttca cctgggtggg tacgtacata ttgagcagcg cgaatcttat caatcagtgc
     7381 actagcgtca cgaccgattc catctgcatt gatttccata gcttgaacga ttccgtctgg
     7441 atcaataata aatgttcctc gctgagctaa tccttgctct tcatccaaaa catcaaaaat
     7501 acgagaaatc tgatgagacg gatcaccgat catcacgtat tcgattttac cgatagcatc
     7561 tgatgtatca tgccatgctt tgtgagtaaa gtgggtatcg gtagagcaag aaaagacttc
     7621 aacacctaaa gatttcaatg tactgtattg ttcttgtaag tcttctaatt cagtaggaca
     7681 aacaaaagta aaatcagctg gatagaagca aagaatactc cagttcccca taagatcttt
     7741 ttcactgact tctaaaaatt ctccttgatg ataagcatgt gcattaaatt cttcgatttt
     7801 tttcccaatc aatgacatag taaaacctcc aataataatt atttatttat aattattgta
     7861 atttagttgg agttgcaaag tcaatggata ttttgttatt taataaagat tactcaaaaa
     7921 cgaaggttaa ttataaaaat agaagaggtc gcaagaaaat gctactgctg gaaatactcg
     7981 ggataacgtt gagtaacagt ttcctgttct ttcagcatca tgtgtaagtg caatcccgtt
     8041 tgaaatcgag cttcttctgg ttttttatca actaaagcct tgagtaaatc ataatgttgt
     8101 tccatgatcg tttcccatgg aagcccctga atggtcactc gtaaccagcg gaaacgttct
     8161 aaatgggtat tatggtcatc taaccagttc caaacttctt ttcttcctgc aatttcgaaa
     8221 caagcttcat gaaataaatt atctgcacgg aaaaaagcgc gttcgtcatt tattttgact
     8281 gctttttctt gttcttctaa aatcgtttct agcactattc gtgtttgtgt gtccaatttg
     8341 gaacaacatt caatcatgat ttgctcttcc aactgttctc ggacaaagcg agcattttct
     8401 gcagaaacaa gattaatttt ggaaacatac gtaccacttt gaggtatcgt atacacgagt
     8461 tcttgctgac gtagttgaat caatgcttcg cgaatgggag tccggccgat agcaagatgt
     8521 tcctccagtg ccttttcaga tattttcctt ccaggttcta agtcagcata gataatttgt
     8581 ttacgaatcg cttgataagc ttgtgattgc aagctgcttt tcatctttat tcctccaaaa
     8641 tcatttctat gaacgtagta taacagaaga ggtctgaaaa acaatctggt atgtcacatt
     8701 aactagtaaa tttgtataaa tagtaaaatt ttggttgaca tagcgatttt tattgcataa
     8761 aattcacttg aatacaaaaa ctaatatgtt agtttaggag gggaaaaatg attgatttaa
     8821 acaactggag gaactttatc cctgaattat ctcaaaaaga agtagaaata cctcattttg
     8881 accgtgcatc attaagagaa aatggacaaa aaaatcctgt ctggatccat ttcggtggcg
     8941 gaaatcttta tcgaggattt catgcagaaa tcgctcaacg tttagcggat ctaggcgaat
     9001 tgtccactgg tgtcattgtt tgtgaaacat tcgatgaaga aataattgaa agggcttacc
     9061 aagattttga tgatttgatt cttgaagtgg tcatgccaag cagtggagag ttcgtcaaac
     9121 gtatattagc tgcgacagct gacagtctgt attgtcatcc agattgttct gaacactttg
     9181 ataagatgat tcgacacttt gaaaatccta gtttacaatt agttactttt gctattactg
     9241 aaaaaggtta tcgattgaaa gatacaaata ataaatatat aggaccgatt gcagtcgata
     9301 taaaaaatgg tccaaagaag ccatcacata caatgagtat tcttgctgtg ctactttata
     9361 aacgtttttt atctggagct tggccgattg cacttgtctc tacagataat ttttcacaaa
     9421 acggtagtca attacgtact tctttgctaa cgatcgcaaa agaatggcaa aaggaaggtt
     9481 atgtacaaaa tacatttatc caatacttat cgaatgaaga aattgttgcg tttccatgga
     9541 cgatgattga tcgtatcacc ccgaatcctt ccttgtcagt tagcaaaatg ttggcaaaag
     9601 agggcttttc aaatatgtcg attttccaaa ccgcaaaagg aaataatatt gctccatttg
     9661 tcaatacaga ggacatccat tatttaatca tggaagatca ttttcccaat ggacgtccga
     9721 aattcgaaaa agctggggta ttcttgactg atcgtgcaac ggtagataaa gcagatacga
     9781 tgaaggtgac gacttgcctt aatcctttgc atacgtcttt agctattatg ggttgtttac
     9841 tggggtatag gagcatagct gaggagatga aagatccaga tttatcgctg ctagtttctg
     9901 aaatcgggta caatgaaggc ctcccagttg tggagtctcc aggtatcatt gatccaaaga
     9961 tctttctgga agaagtaatt actgtgcgct tttctaatcc aaatattcct aatatgcctc
    10021 agcgaatcgc cacggatacc tctcaaaagg tggcgattcg ttatggtgag acgattaaaa
    10081 aatatatgga aatagaagtt aaagacccgc agacacttgt attcatacca ttgactctcg
    10141 ctgcttggct tcgatacctt attggcattg atgatcaagg aaaaccattc atcccgagtc
    10201 cagacccact attgacagac cttcagcata agctttccga tattcaactg ggtacgcaag
    10261 atacatcaca tattcatgag aaaatggctc ctattttgtc aaataaagaa atttttggtg
    10321 tagatcttta ccaagcagag cttggcagaa aaatagaaga aatgttcatc aagatggtag
    10381 caggaccaaa tgccgtacga aaaacattac aggattactt acaagaacat ggaggaaatt
    10441 tatcatggaa atgactttta gatggtacgg atcacaggaa gaaaagatta ccttagatga
    10501 aatcagacag attcctgcgg tcaaagggat tgtaggcaca ttgatggata taccagtagg
    10561 agaggtatgg cctagggaga ggatcaaaga actaaaagaa gaaattgagt ctcgtgggtt
    10621 aacactaaaa gtaattgaaa gcgtcaatgt gcatgatgcc attaaaattg gattaccttc
    10681 ccgtgagcag tatatcgaaa actataagca gacaatccga aacttggcag aatatggaat
    10741 agaagtcatc tgctataatt ttatgcctgt atttgactgg gtgaaatctg accttgacta
    10801 ccgcttagaa gacggctcat ccacgttagc ttttatatct gatgatattc cagatgatcc
    10861 aagagaaatc gtcgcgcgca tcgaacattc cagcaatgaa tttggattgc cgggatggga
    10921 acctgagcgc ttagctccta tcaaatcctt gcttgaagcc tatgcatcag tagacgaaga
    10981 aaaacttcga gaaaattttg cctattttct ccaatcaatc atcccaacat gtgaagaggt
    11041 aggggtcaaa atggctgtac atccagatga tccgccatac tcgatttttg gattagctcg
    11101 aatcgtaaag aatcgagaag atttagattg gttgtgtcgc atagtagaca gcccttataa
    11161 cggaatcact ctttgtacag ggagtattgc agaagatcca gaaaatgatg tatatgggat
    11221 tttagcagaa ttttgccaaa gagatcgaat cccatttgca catgttcgaa atattaaatt
    11281 tttagctaat cgagatttct atgaggcacc acataaatcc gaatacggtt cattagacat
    11341 gtaccgcata ctaaaaacaa tgtatgacaa cggatttaac ggatacatcc gtcctgatca
    11401 tggaagaatg atctggaatg agacaggaag accaggatac ggcttatatg atcgagcatt
    11461 aggtgctgtt tatctaaatg gattatggga agcaatcgaa aaaggaaaca aaagtaaaga
    11521 taattagtga aaaaaactgt aattttatta tgtaaataag aaggatgttc aaattcttga
    11581 tacataaatc cggaggttgt taataagcat tcggatttat gtatcttatt tggtctagga
    11641 agtaacttga ttttcttttt tcgattgatc catgagtgct tcttctttta attcaaagat
    11701 atattctgag atcctcataa tcactttttt ccagtcttct ggtgtgatga ttccttttaa
    11761 aatatagatt tttttgatat gagaaggcaa ttgtttcaaa gggcagtcgc tgataacgat
    11821 gttgcagttc tccataaaat cattttgtat tttgatcgat tcatttgaaa aaatttgttc
    11881 tatcttaacg atcagatctt tactgatata aaaatcacgc gtatagttca caccaatcac
    11941 aattcgcggc tgttttttgt agcgatcata caaatgtact aggtcttcaa ccatccattg
    12001 cacattttcc ttttctaaaa taactggtaa caagtgttga ttttgttctc tgaaatccat
    12061 ataaaattgt tcaatggcgg tttggacttc tttcaatacg ctatgttctt gataaagcat
    12121 gaaagtagga aggggcgtat ttggttgatt gaagttaaga aatccattat aaagtttatt
    12181 tcgtgcgaat gccaatgagt aaagaatctt ttctttttca ggtataaaca aatcaaatac
    12241 agtacaaagg gactccacca tttctcttga ataaagaaca tgaggaacgt ttgctttttt
    12301 taaaagtttg tattgctcca agcttgtttc ttccgattcg attttcgtga tagaaagtcg
    12361 agctaaaata ttcactaaga tgcgttgttc gcgacaaatt ggcagattag gtcgcaaaat
    12421 atctacagag agatcaataa aaaagtcaat atattcatac tctgtgaatt ctttttgtac
    12481 gtctttcaca gaagttgcag gaaaattctc tttac
//
LOCUS       DAZKKW010000077        12502 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.079, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000077 DAZKKW010000000
VERSION     DAZKKW010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12502
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.079"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            159..731
                     /locus_tag="ACG8PO_002106"
     CDS             159..731
                     /locus_tag="ACG8PO_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HFQ0207404.1"
                     /translation="MTENKATQERTPQERLQEEQEHKEHVHHTKINAAAIADHLLGNM
                     HTLHVKLHQYHWYVKGANFFTLHEKFEELYNENEKWFDKLAERLITSGHKPASTTVEF
                     EKYSMLSEDAVNKYAKAEDMVENIIEDFRSTRDLTIRAIRLAQDEGDDALEDTLIAFK
                     NDLDKNIWMLQAFIGKEALEDDDALDEDED"
     gene            876..1580
                     /locus_tag="ACG8PO_002107"
     CDS             876..1580
                     /locus_tag="ACG8PO_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011476586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFQ0207405.1"
                     /translation="MTKHTHHEIGDHAACIRLVPIFNHLEDSAMNYIAEKAHTKQYQK
                     GEFLFRSQEKEDTLYIINRGKIRIYRLADSGKEQLVRILNPGDFTGEWTLFNPDAVHE
                     EYAEATRDTVVCMIQQKDVQDFLEQYPAISLKLLAEMSKRLESSERQTTQVAVESVIT
                     RLVLFLAENVEPEMGNSPTITLPMAKKDIASYLGTTPETISRKFGELEDEGLIQQLSG
                     KRIKIQDLDDLLLYSE"
     gene            1808..2146
                     /locus_tag="ACG8PO_002108"
     CDS             1808..2146
                     /locus_tag="ACG8PO_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0207406.1"
                     /translation="MHCFWVIVAITLSAAGAFFVVPFTKNTIGGEAKTSNVVEADRLA
                     MEKEEEVVKGAGVIGHGKVAYYEASDQGDKSVFTVWFPWSLAGILFSITMTRMTRKIE
                     EMEQIEWDSY"
     gene            2131..2985
                     /gene="lgt"
                     /locus_tag="ACG8PO_002109"
     CDS             2131..2985
                     /gene="lgt"
                     /locus_tag="ACG8PO_002109"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFQ0207407.1"
                     /translation="MGFVLGVLNPHVVEIGSVVIHWYGVIIAAGILAALQLSTKEAKK
                     KGLEEDTIIDMALWAIPIGLLGARLYYVLFELGYYLQNPGQILAIWNGGIAIYGGLIA
                     GGGTLYWYAKKKGTSLALVLDILAPNVLLAQSIGRWGNFMNQEAHGGPVTRQFLENLY
                     LPEFIIEQMNMNGTYYHPTFLYESLWSLLGFVLIVTIRNRKQLLRQGEVALSYVLWYS
                     VGRFFIEGMRTDSLWIGEWIRVSQALSLALFIGAIVVWFIRRQDYPPISYYSDGNKGQ
                     KKTIKMVN"
     gene            3132..3365
                     /locus_tag="ACG8PO_002110"
     CDS             3132..3365
                     /locus_tag="ACG8PO_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HFQ0207408.1"
                     /translation="MEKAILQLETLSCPSCMQKIEGAVKSVNGIDKDSIKVLFNSSKV
                     KTNFDSAVTSIEEIQKAIENVGYPVLKAKVKAV"
     gene            3563..5467
                     /locus_tag="ACG8PO_002111"
     CDS             3563..5467
                     /locus_tag="ACG8PO_002111"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFQ0207409.1"
                     /translation="MQQYILSKKNVITVISGLLIVLGFFSHFVLENVGLSEWSLIIAS
                     VFGITPIAIQAFQAMKVKVISIDVLVSIAAIGALFIQNYEESAIVTFLFLFGHYLEQR
                     TLNQTRSAIKELTEMAPESALKQMDNGEFEEVEVDDIDEGDILLVKTGAKVPVDGIVL
                     TGEGHINEASITGEAIPVNKLADAEVFAGTILENGTIQIRADRVGEDTTFGKIIELVE
                     EAQDSKSEAERFIDRFSKYYTPAVLVLAIVVWAFSQNIELAITILVLGCPGALVIGVP
                     VSNVAGIGNGARNGVLLKGSEVIQDFSNVDTIVFDKTGTLTVGNPTVAATELYEKDPA
                     ETLGYLASVERESDHPLAKAVLNQIGETNFYPVEGTEVVKGGGIVSSVAGHRVAVGNV
                     ALMEKENVTLSKKVQKDVKRFEQQGNSLVLTAVDGELKVLMGIRDQVRPGVKANLQEL
                     KDLGVKNLVVLSGDNQGTVDVVAGELGLTEAHGYMLPEDKSAYIGKLQQRGQIVAFVG
                     DGVNDSPSLALADIGIAMGSGTDVAIETSDVVLMNSNFSNLPHALGLVKATANNMKQN
                     IVISIGVVLVLLTSVFFSEWMNMSIGMLVHEGSILVVIFNGMRLMKYKLKGRKEQKEV
                     SAPAEKVKIS"
     gene            5777..6138
                     /locus_tag="ACG8PO_002112"
                     /pseudo
     CDS             5777..6138
                     /locus_tag="ACG8PO_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342386.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
     gene            6155..6766
                     /locus_tag="ACG8PO_002113"
     CDS             6155..6766
                     /locus_tag="ACG8PO_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CadD family cadmium resistance transporter"
                     /protein_id="HFQ0207410.1"
                     /translation="MLQSIFSAIAVYISTSIDYLFILLIIFSQSHTKKGIRHIFWGQY
                     LGTGILVAVSLFAAYVLNFIPQDWIIGLLGLIPIFLGIRVAFVGEEEEEEEEVVEKLE
                     SRGTNRLFWTVALITIASGGDNLGIYIPYFTSLAFSEIIIALIVFAISVAVLCYISYK
                     LAKISFVSETLEKYERIIVPIMFIALGIFIMIENGTIQTLLGF"
     gene            complement(6868..7776)
                     /locus_tag="ACG8PO_002114"
     CDS             complement(6868..7776)
                     /locus_tag="ACG8PO_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFQ0207411.1"
                     /translation="MSKSDKDHNHSNQNQPKKNIKIAFFINLIFSISEFFFGFLFNSV
                     SIMSDAVHDLGDSISIGFAWFFQGYSEKKQDERFTFGYNRFSLLGALITGVVLISGSF
                     FMIYRSVPRLIDPQPVNYSGMFWLSLVAIALNGYAAWILSKGTSKNESMLNLHMLEDV
                     LGWIGVLIVSILMNFTEAYRLDPILSILIALYILYKTVPEFFTTMKILLNGSPENVNV
                     KSLADSIKNVPAVKDLSHFHIWSLDGEENALIITVLIDPSDIDKAREIKEEIAEVAHK
                     SSVENHITIEITTSKEELEKGYSHNT"
     gene            complement(7818..8132)
                     /locus_tag="ACG8PO_002115"
     CDS             complement(7818..8132)
                     /locus_tag="ACG8PO_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFQ0207412.1"
                     /translation="MDIKTTSPIDKGSILSISKLFKVISDPTRLSVLFLLQKKECSVG
                     NIAISLDMEQSAISHQLKTLKDARLVKSRREGKSMLYSLDDLHVFSILEQVLTHVNEL
                     EK"
     gene            8431..10362
                     /locus_tag="ACG8PO_002116"
     CDS             8431..10362
                     /locus_tag="ACG8PO_002116"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFQ0207413.1"
                     /translation="METIQEQQSQEKHNHNHNHDHDHDHGKMPVVLYFVGLALAVIAL
                     FLNEDYQLVQNVLFSIATISAGYHVILLEGLGETIENSKAQKKFIPNSHILMGLAAIG
                     ASLMGNFWEGTLLILIFSGAHFLEDYAEGRSKREITKLLEMNPTTARLILPDGSTKNV
                     DVSELKVGDQLQVLNGDQVPIDGVILSGSTSIDESSITGESIPKEKSKGDGVFGSTIN
                     GTGTFTMKVTKENKDTVFSKILQLVNQNQDNQTKAASIIQKFEPKYVTVVLIAIPLFM
                     LLAPFLLDWTWSQSVYRGLVLLVAASPCALAAATVSATLSTTSNLAKKGVLSKGSSYL
                     SQLADIKAIAFDKTGTLTKGKPEVTNYYFADSVNEENMIDIVVALEKESNHPLADAIL
                     RKFEQKNKLTIEVENQIGKGLTGDYKGRNYRIGKPTSFDDVSDEYSRLNNEWASEGKT
                     VVYVAVDEEVIGLIALMDVPSEYAKETIEYFRKQGIHTTLITGDSEMTGKAVAKQLGI
                     DEVIANVMPEDKSRIIDEQKEKYGVTAMVGDGVNDAPALVNADVGIAMGDGTDVAVEV
                     SDLVLMQNNLSKLVQSHNISSKMNRVIWQNIIFSMAVVAFLIVVSFLGLTDIAISVII
                     HEGSTLVVILNGLRLLKAV"
     gene            10655..11206
                     /locus_tag="ACG8PO_002117"
     CDS             10655..11206
                     /locus_tag="ACG8PO_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0207414.1"
                     /translation="MGYNVQPLRSQQEINDFLFCLRRNKNAERDVFLFLIGINSGLRM
                     SDIVKLKKKDVITSKNPRIVEQKTGKTRILYLSSLQDLIHGYTAPLDPEAYLFPSTKG
                     GHLEVNTVYQMFQKVAKLLGRDDIGTHTLRKTFGYHYYKKTKDVATLMEIFGHSSEKI
                     TKRYIGINEDEISETLLNFRLGF"
     gene            11519..12061
                     /locus_tag="ACG8PO_002118"
     CDS             11519..12061
                     /locus_tag="ACG8PO_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBECR4 domain-containing protein"
                     /protein_id="HFQ0207415.1"
                     /translation="MSIKETTQITRQLNAIYKAAHLLQEHFVDKKVSFVGEVSTVAII
                     FSTTNFMHLCGIDYRRGTHLFFQDALDRKINLQDIQIKTDGTTFQKLQVIGSLDLLLG
                     KHISIVGRGVYSSLRYDAAIRTRKKILALSLKQNGLIYIPISLLNLSSKEIGPGQKVT
                     GIFSEDLTSGELKMIMEVID"
BASE COUNT         4049 a         2093 c         2613 g         3747 t
ORIGIN      
        1 gaatttaata gagaagcctg gcgtctatta agaaataatt ttcttgatga gcgtcaattt
       61 ttttaacgga aatctatcct atactaaatt cagtaaagaa aaaggaggaa tcaaagaaag
      121 ctattttaaa caaaccaaaa ttataggagg aataaatcat gactgaaaat aaagctactc
      181 aagaaagaac accccaagaa agattgcaag aagaacaaga acacaaggaa catgttcatc
      241 atacgaaaat taatgcagcg gccattgcgg atcatctttt aggcaacatg catacattac
      301 atgtgaaact gcaccaatat cactggtatg taaaaggagc caatttcttt acgttgcatg
      361 aaaaatttga agagttgtat aacgaaaatg aaaaatggtt cgacaaactt gcggagcgtc
      421 taattacttc tgggcataaa cctgcttcga caacggttga atttgaaaaa tattcgatgc
      481 tttcggaaga tgcagttaat aaatacgcta aagcagaaga catggttgaa aatattatcg
      541 aggacttcag aagcactcgt gacctaacta ttcgcgcgat tcgtttagct caagacgagg
      601 gtgacgatgc tttagaagat acattgattg cttttaaaaa tgacttagac aagaacattt
      661 ggatgctgca agcgttcatt ggtaaagaag cattagaaga tgatgacgct cttgatgagg
      721 atgaagatta aggtaaaagt agaagctgat ttagggattg gaattctttc cagtcctttt
      781 tccattttct tgatgtgcat caattttttt gtagtaagag acttatataa taaaagcagc
      841 gaatataaac aaaataaata aagggggaga atgaattgac taaacatacc catcatgaaa
      901 ttggcgacca cgcagcctgt atccgcttgg ttccaatatt caatcactta gaggatagcg
      961 cgatgaatta cattgctgaa aaagcccaca cgaaacagta tcaaaaagga gagtttctgt
     1021 ttcgttcaca agaaaaagag gatactttat atattatcaa tcgtgggaaa atccgtattt
     1081 atcgattggc agactctggc aaagagcagc tagtgcggat cttgaaccct ggtgatttca
     1141 ccggagaatg gaccttgttc aacccggatg cagtacacga ggaatatgcc gaagcaaccc
     1201 gagataccgt tgtctgtatg attcagcaaa aagatgtcca agattttctg gaacaatacc
     1261 cagctatttc tttaaaacta ttggcggaaa tgtctaaacg gttagaaagt tcagaacgtc
     1321 aaacgacaca agtagccgtg gagagtgtca ttacccgttt agttttgttt ttggcagaaa
     1381 atgtggaacc tgaaatgggc aactctccca ccatcaccct gccgatggca aaaaaggaca
     1441 ttgcttccta tttaggaacg acacctgaga ccatcagtcg caaatttgga gaattggagg
     1501 acgaaggatt gattcaacag ctgtctggga aaaggatcaa gattcaggat ttagatgatt
     1561 tattgcttta cagcgaataa tcggacacac ttttttctac acgttggtgt tctgtcgctc
     1621 ttcattaaca acgggacaga actcagtgct tttgcttcca tctgtccgtt tttggctttt
     1681 ttaagacaga acgaccttca ttcattggaa aaataaaagc acaaacatga taaccctcaa
     1741 gtttttttgt tttatttttc tttaagatag ggttgtcgaa caaaacgtaa aaataagtag
     1801 ggtgaatgtg cattgttttt gggtgatagt tgccatcacg ttgtcagcag cgggggcatt
     1861 ctttgtcgtc cctttcacca agaacacgat tggcggcgaa gcaaaaacgt ctaacgttgt
     1921 tgaggcagat aggttagcta tggaaaaaga agaggaagtt gtaaaaggag ccggcgtaat
     1981 cggacacgga aaagtggcgt attacgaagc gagtgaccag ggagataaaa gtgtattcac
     2041 tgtttggttt ccatggagct tagccggaat cttgttcagc atcaccatga ctagaatgac
     2101 gcgaaaaatt gaagaaatgg agcaaataga atgggattcg tattaggcgt tttgaatccc
     2161 catgtggtgg agattggttc tgttgtgatt cattggtatg gagtcattat tgcagcgggc
     2221 atattagcgg ctctccagtt gagcaccaag gaagcaaaga aaaaaggact ggaagaggat
     2281 accattatcg atatggcctt atgggcaatt ccgattggac tcctcggcgc tcggctttat
     2341 tatgtgttat ttgaacttgg ttattatctg caaaatccag gacaaatcct tgcgatttgg
     2401 aatggcggca ttgcgattta tgggggattg attgcaggag ggggtacgct atattggtat
     2461 gcaaagaaaa aaggtacttc tttggcactc gttcttgata ttttagcgcc caatgtattg
     2521 ttagcccagt ccattggtcg ttggggcaat tttatgaatc aggaagctca tggtgggccg
     2581 gttactcgtc aattcttgga aaatctttat ttacctgaat ttatcattga acaaatgaat
     2641 atgaatggta cctactacca tccgactttt ttatacgagt cattgtggag tttgttgggc
     2701 tttgtcctca tcgtcactat acggaaccga aaacagcttt tgcgtcaagg agaagtcgct
     2761 ttgagttacg ttttgtggta ctcagtgggt cggttcttca ttgaaggtat gcgaacggac
     2821 agtttatgga ttggcgagtg gatccgcgtt tcgcaagcct tgtctctggc cctgtttatt
     2881 ggcgcgattg tagtatggtt tatacgcaga caagattatc caccaatttc ttattattct
     2941 gatggcaata aggggcagaa aaagactatt aagatggtga attgagcaaa aagataaaaa
     3001 gaagtgcaaa acttgatatg cgtcaatttt cgtttgctaa aagtgagcta tactacagtt
     3061 gtaaagagga aaaggcagtg ttcattaatc tgctgccgat aaaataaaaa cacataaagg
     3121 agataggaat tatggaaaaa gctatattgc aattagaaac gttgtcttgt ccaagttgta
     3181 tgcaaaaaat tgaaggtgcc gtgaaatcag ttaacggtat tgataaagac agcattaaag
     3241 tattattcaa ctccagcaaa gtcaaaacca attttgattc agctgtcact tcgattgaag
     3301 agattcaaaa agctatcgaa aacgtaggct atccggttct taaagcaaaa gtcaaggccg
     3361 tttaacagat taaatgaact aggcaattct ggaagaaaag ctatttttcc aggattgctt
     3421 tttttgttgc aatccgattc gaggtgctta gaaacgataa aagagaactt tctaaacttg
     3481 atgtcagtca attaattact taggcaatta tcttatactg tgtttatcaa taaaagagaa
     3541 attaaatttg gagggaaaaa gcatgcaaca atatatatta agcaagaaaa atgttatcac
     3601 cgtcatcagt ggattgttaa tcgtactcgg tttcttcagt cactttgttt tagaaaacgt
     3661 aggactttca gagtggtctt tgatcatcgc ctctgtcttt gggattacgc caattgccat
     3721 tcaagcgttt caagcaatga aagtcaaagt catcagtatt gatgtcttag tcagtattgc
     3781 agcgattggt gcgcttttta ttcaaaatta tgaagaatcg gctattgtca cgttcttatt
     3841 cttattcgga cactacttgg aacaacgaac attgaaccaa acgcgatctg ctatcaaaga
     3901 attgactgaa atggcacccg aaagcgcctt gaaacaaatg gataatggcg aatttgaaga
     3961 agtagaagtg gatgatatag atgaagggga tatcttgctc gttaaaacgg gtgcgaaagt
     4021 tccagtagat ggcatagtcc ttacgggtga agggcatatc aatgaagcta gcattacagg
     4081 tgaagctatt ccggtcaaca agctagctga tgcagaagtt tttgcaggaa ccattttaga
     4141 aaacggaacg attcaaatca gagctgaccg agttggtgag gacaccacat ttggaaaaat
     4201 tattgaactc gtggaagaag cacaagattc taaatccgaa gcggaacggt tcattgatcg
     4261 cttttctaaa tactacacac cagctgtctt ggttctggca attgttgtat gggcgttcag
     4321 tcaaaatatt gagttagcaa tcaccatctt ggttctaggt tgcccaggcg cattagttat
     4381 cggagtgcct gtctcaaacg ttgccggtat tgggaatggt gctcgtaacg gtgttctttt
     4441 aaaagggagt gaagtcattc aagacttcag taatgtggat acaattgtat ttgataagac
     4501 aggtacttta actgtcggaa acccaaccgt tgcagcgact gaactgtatg aaaaagatcc
     4561 tgctgaaacg cttggctatc tggccagtgt ggaacgggaa tcagatcatc cattagcaaa
     4621 agcggtctta aatcaaattg gagaaacaaa cttttatcct gttgaaggaa ctgaagttgt
     4681 gaaaggcggc ggaatcgttt caagtgtagc tggacataga gtggctgttg ggaatgtggc
     4741 cttgatggaa aaagaaaatg tcactctcag caaaaaagtg caaaaagatg tcaaacggtt
     4801 tgaacaacaa gggaactctc tcgttttgac agcggtcgac ggtgaattaa aagtactgat
     4861 gggcattcgc gatcaagtcc gtccgggtgt gaaagctaat ttgcaggaat taaaagactt
     4921 gggcgtgaaa aatctagtcg ttctttcagg agataaccaa ggaaccgttg atgtggtcgc
     4981 cggcgaactt ggtttgaccg aagctcacgg ctacatgttg ccggaagaca aatcggctta
     5041 tatcggaaaa cttcaacaac gtggtcaaat tgtagccttt gttggagatg gcgttaacga
     5101 tagtccgtcc ttagctttag cagacattgg aatcgcaatg ggaagcggaa cggacgtagc
     5161 gattgaaaca tccgatgtcg ttttaatgaa ctcgaacttc agcaacttgc ctcacgcatt
     5221 aggattagta aaagccaccg caaataatat gaaacaaaat atcgtgattt caattggagt
     5281 agtgttggtc ttgttgacca gcgtcttctt cagcgaatgg atgaatatgt ctatcggaat
     5341 gttggttcat gaaggtagta tcttggtggt aattttcaat ggcatgagat taatgaagta
     5401 taagttgaaa ggtcgaaaag aacaaaaaga agtatcagca cctgcagaga aagtaaaaat
     5461 atcttaagaa taaattagaa gaggctgaga caatagtcca tccttgaaat gacaaaagcg
     5521 gaaactcaga tttttaaatc tgggattttc cgcttttttg agttcttgaa aaaaaataag
     5581 agtatagagt atgttcttta taaaattaat tttcgtgtaa atgattaaaa aatttaataa
     5641 taaaatttaa attcctatta agttgtaaca catgaaattg cttataatag tgtattgaaa
     5701 ttaaggtaga ttgacaaacg agtgatcaaa tagatatact accttcaaac gaccgtttga
     5761 atgtgaggtg agatgaatgg aaaaagtttg ccaagtgact atcgtacata aagaaaaagt
     5821 aaatgtaata aaaaaaaatt ggatcaaaaa gatttttcaa acttacttgt gttagggaaa
     5881 tgtttcagcg attcttctag gattaaaatt ttctatgctt tagaaaccta taaggaaatg
     5941 tgtgtctgtg atttagcaga aatacttact gctagtgtcg ctacaacgtc acaccattta
     6001 cgttttttaa aaaaacatgg aatggcaaaa tcacgtcaag atggaaaagt agtttattat
     6061 tctttagcga atgaagacat cctttccgct attcgtgttt ttttaaagtt atcagaaaat
     6121 ctatctatga aatcttagtt tggaggaagt tattttgtta caaagtattt tttcagctat
     6181 cgctgtttat atttctacca gtattgatta tttgtttatc ttgctgatta tcttttctca
     6241 aagccacaca aaaaaaggta ttcgtcacat tttttggggt caatatcttg gaacaggtat
     6301 tttggtagcc gtaagtttgt ttgctgctta tgtgctaaat tttattccgc aagattggat
     6361 cattggtctt ctcggtctaa ttccgatttt cttaggaatc agagtcgctt ttgttggcga
     6421 agaggaggaa gaagaagagg aagtcgttga aaaactcgaa tctagaggaa ctaatcgctt
     6481 attctggaca gttgccttaa taacgattgc ttctggaggc gataatttag gtatttatat
     6541 tccatacttt acctcgttag ctttttcaga aatcattatt gctttaatcg tattcgctat
     6601 ctctgttgcg gttctctgct atatcagtta caaattagcc aagatttctt ttgtttccga
     6661 aacgttagaa aaatacgaac gaatcattgt ccctataatg tttattgcgt taggaatttt
     6721 tattatgata gagaacggga cgatacaaac gctactaggt ttttaaaaat cataatcacg
     6781 aaagataaaa agagaggcag cgactaatca aaatcgctgc cttttttgct tttcaaataa
     6841 tgaagtattt tttagcgtag aactatctta cgtgttgtgg gagtatccct tttcaagctc
     6901 ttctttgctt gtagttattt ctattgtgat atgattctct acgcttgatt tgtgtgcgac
     6961 ttctgcaatt tcttctttta tttccctagc cttgtctatg tctgaagggt ctataagaac
     7021 cgtgatgata agggcgtttt cttccccgtc cagtgaccaa atgtgaaaat gtgagaggtc
     7081 ttttacagca ggaacatttt taatggagtc cgccaagctc tttacgttta cattttcagg
     7141 cgaaccattc aataaaattt tcattgtggt gaaaaattca ggaacggttt tgtagaggat
     7201 ataaagtgca atcaaaatcg ataagatagg atccagacga tatgcctcgg taaagttcat
     7261 tagaatactc acaatcaaga ctccaatcca ccctaatacg tcttccagca tatggaggtt
     7321 caacatgctc tcgtttttgg atgtgccttt gcttaaaatc cacgcagcat atccattcaa
     7381 tgcaatagct acgagagaaa gccagaacat gccactataa tttacgggtt gtgggtcaat
     7441 caaacgtggc acactacgat agatcataaa aaacgagcca ctaattagca caacgccagt
     7501 tatcagcgcg cccagtaaag aaaagcggtt atagccaaaa gtgaaccgtt catcttgttt
     7561 tttctctgaa taaccttgaa aaaaccaagc gaatccaatt gaaatagagt cccctaaatc
     7621 atgaactgcg tcggacataa tggacacact attgaaaaga aagccaaaga aaaattcgct
     7681 tatagagaaa ataagattta tgaagaaagc aattttaata ttctttttcg gttgattctg
     7741 attcgagtga ttatggtctt tatctgattt tgacatttaa aaacctccta agtttgtatg
     7801 ctacatacta attggtttta tttttctaat tcattaacat gagttaagac ttgttcgaga
     7861 atactaaaaa catgaaggtc atctaaactg taaagcatac tttttccttc tcttcttgat
     7921 ttcactaagc gtgcgtcttt caacgttttt aattgatgag aaatagcgga ctgttccatg
     7981 tccagagaaa tagcgatatt tccaacacta cactcttttt tctgtaaaag aaagagaacc
     8041 gacaatcgtg taggatcact aatcacctta aatagtttgc ttattgacag aatagatcct
     8101 ttatcgattg gagaagttgt ttttatatcc atagtgtttt tattcctttc atatgacctt
     8161 ccgttcatat ctatattacc atatgagcga aacaatgcta atcttttgtt tataaaagag
     8221 aagtggcgtt ctctttatct tgacaaaatg tatatagcga tataaaatga gtatacaaac
     8281 atatgattaa ttagtcatat gtaaggtgga ttcattacga tttacttttg gaataaacga
     8341 aaggagtcag aaatagcaga aatttatgtg attcaagaag taaatgatgg aaaacaaagg
     8401 aattataaat tatataggga ggcaatagaa gtggaaacta tccaagaaca acagtctcaa
     8461 gaaaagcata atcataatca caaccacgat cacgaccatg atcatgggaa aatgccagtc
     8521 gttttgtact ttgttggttt agcattagca gtgattgctc tatttttgaa tgaagattat
     8581 cagttggtac aaaatgtttt gttctcaatc gctacaatca gtgccgggta tcacgttatt
     8641 cttcttgaag gccttggaga aacaattgag aattcaaaag cacaaaaaaa gttcatacct
     8701 aattctcata ttttaatggg attagcggca attggagctt ctttgatggg gaatttttgg
     8761 gaaggaacat tattgattct tattttttct ggcgcacatt ttttggaaga ttatgcagaa
     8821 ggaagaagca aacgagaaat aacgaaatta ctcgaaatga atccaacgac tgcccggtta
     8881 atcctgcctg atggtagtac aaaaaatgtg gatgtcagtg aattaaaagt gggagatcaa
     8941 cttcaagtgt tgaatggcga tcaagtacct attgatggcg tcattttgtc tggatctaca
     9001 tccattgatg agtcgtctat tactggagaa agtatcccga aagagaaatc caaaggagat
     9061 ggcgtcttcg ggagtacaat caatggaaca ggtaccttca cgatgaaagt caccaaagaa
     9121 aacaaagata cagtattttc aaaaatttta caattagtga atcagaacca agataaccaa
     9181 acaaaagctg cgagtattat ccaaaaattt gaaccgaaat atgtcacagt tgttttaatt
     9241 gccattcctc tattcatgtt attggctcct tttctacttg attggacatg gtcacaaagt
     9301 gtctatagag gattggtcct tttagttgca gcttcaccgt gtgctttagc agcagctact
     9361 gtctcggcaa cgctatcgac gacttctaat ttagccaaaa aaggagtcct ttcaaaaggt
     9421 agttcttacc tgtctcaatt agccgatatt aaagcaattg catttgataa gacgggcacc
     9481 ttgaccaaag gaaaacctga agtaaccaat tattattttg ctgattccgt gaacgaagaa
     9541 aacatgattg atatcgtagt agctcttgaa aaagaatcca atcacccctt agcagatgct
     9601 attctaagaa agtttgagca aaaaaacaaa ctaactattg aagtagaaaa ccaaattggt
     9661 aaaggattga cgggagatta caaagggaga aattatcgta taggaaaacc gacttcgttt
     9721 gatgatgttt cagatgaata tagtcgttta aataatgaat gggcgtcaga aggtaagaca
     9781 gtcgtatacg tagcagtaga tgaagaggtt ataggcctta ttgcccttat ggacgttccg
     9841 agtgaatatg ctaaagaaac cattgaatat tttaggaaac aaggtataca caccacatta
     9901 attactggtg actcagaaat gacgggaaaa gcggttgcta aacaattagg aatagatgaa
     9961 gtgattgcga atgtcatgcc ggaagacaaa tccagaatta tagacgaaca aaaagaaaaa
    10021 tacggcgtga ccgccatggt tggagacggt gtaaatgatg ctcctgctct tgttaatgca
    10081 gatgtgggaa tcgctatggg agatgggact gatgtggcag tagaggtatc tgatttagtt
    10141 ttaatgcaaa acaatttatc taaattggta caatctcata atatttcttc gaaaatgaac
    10201 cgtgttattt ggcagaatat tattttttca atggccgttg ttgctttttt aattgtcgtt
    10261 agtttcttag gcttaacgga tattgcaatc agtgtcatta ttcatgaagg aagtactttg
    10321 gttgttattt tgaatggact tcgattgcta aaggcagtct aaagaatgat tgcacaactt
    10381 caaattaatc gagtaataga gataattaag gattacacta cattttttat ctctattact
    10441 tgtttatatt tatctagatg ttcaaagtca aaatgatata tcaaccatac tcttgtggcg
    10501 cgggttgaat ataagataca tgaaattgga taaaatcatg tatcaagaat cctatcctta
    10561 tagggttctt tttttttact ccccctaaac acacgatttc gtgttgtaat aactataatc
    10621 atgtattcga acagactaaa aaggagggat tgtcatgggc tataatgttc agccactacg
    10681 gtcgcaacag gagattaatg attttttatt ttgcctgcga cggaataaaa atgcggaacg
    10741 tgacgttttc ttgtttctaa ttggaattaa cagcggtctg cgcatgtccg acatcgttaa
    10801 actcaagaaa aaagacgtga ttacctcaaa aaacccgcgc atcgtggagc aaaagacagg
    10861 gaaaacacgc attttatact taagcagctt acaggacttg attcacggct ataccgcacc
    10921 cttagatccg gaggcttact tgtttcccag tactaagggc ggccatttag aagtcaatac
    10981 ggtctaccag atgtttcaaa aggtcgcgaa gctgttagga agggacgata tcggcaccca
    11041 caccctacga aaaacctttg gttaccacta ttacaagaaa accaaagacg tggccacctt
    11101 aatggaaatc tttggtcaca gcagcgagaa gattaccaaa cgctacatcg gaattaatga
    11161 agatgaaatc agtgaaacgt tattaaattt tcggttaggt ttttgattgt attatataat
    11221 agataagcac agtaagacgg ttataatcac ccacagtatt ggacaaatca accttttttt
    11281 ggtatattat atatacaaat gagagcaggc tcatagtcaa cttgatataa atccggaaca
    11341 taagggtccg gcgggtggcg agcaccaaaa ctaaactcga tacaaatccg gaacgtaagg
    11401 gtccggcggg tggcaagcac caaaactaaa cctgtacaaa aggctgctct tttgagcagc
    11461 tttttttatt gggaaaaagg agcattcttc gcacgcggca aaaaaagaat acaaacgcat
    11521 gtctataaaa gagacaaccc aaattaccag acagttaaac gcgatttata aggctgctca
    11581 tctcttacaa gaacattttg tagataaaaa agtatccttt gtcggagaag tctcaacggt
    11641 agcgattatt ttttcgacaa ctaattttat gcacctctgt ggcattgatt atcgcagagg
    11701 cacacacttg ttttttcaag atgccttaga caggaaaata aatttacagg atattcaaat
    11761 caaaacggat gggacgacct ttcagaaatt acaagtaata ggtagccttg atttattatt
    11821 ggggaagcat atttctattg ttggcagagg tgtttactct tctcttcgct atgatgcagc
    11881 tattcgaaca aggaaaaaaa tattggctct gtctttgaaa caaaacggat taatctatat
    11941 ccctatctct ttgttaaacc tatcatcaaa ggaaattgga cctggacaaa aagttactgg
    12001 catttttagc gaggatttaa catccgggga acttaaaatg attatggaag tcatcgatta
    12061 atcattaata gtgatatgcg acgtcatttt gaagtaatta ttctattttg ggttataaaa
    12121 taaagcattt taagacgatt tcaaatcatt catgtgggtt atttatcacg gatgagcttg
    12181 aaatcgtctt ttatacgtat caaattgaaa tgttcggcat acaacctatc gttgttacta
    12241 cattactaat tgtcacttgt taaggaactt ggatcctttc taaacattcc ctagagataa
    12301 attttacttt atccctccgt taatatccaa tttaagcacc tttccgattc agggttaatt
    12361 tgcatatcaa gtgcagcttg tgttctagta agattagccc ttttagtccg gctgagttga
    12421 cttatggata taagatatgg tcatagcaaa tagatactac gtatagtctg ttaatttagt
    12481 gtcattaatc ttaagttgga ta
//
LOCUS       DAZKKW010000078        12203 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.080, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000078 DAZKKW010000000
VERSION     DAZKKW010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12203
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.080"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(83..640)
                     /gene="frr"
                     /locus_tag="ACG8PO_002119"
     CDS             complement(83..640)
                     /gene="frr"
                     /locus_tag="ACG8PO_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFQ0207416.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(643..1365)
                     /gene="pyrH"
                     /locus_tag="ACG8PO_002120"
     CDS             complement(643..1365)
                     /gene="pyrH"
                     /locus_tag="ACG8PO_002120"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFQ0207417.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(1501..2382)
                     /gene="tsf"
                     /locus_tag="ACG8PO_002121"
     CDS             complement(1501..2382)
                     /gene="tsf"
                     /locus_tag="ACG8PO_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFQ0207418.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(2481..3263)
                     /gene="rpsB"
                     /locus_tag="ACG8PO_002122"
     CDS             complement(2481..3263)
                     /gene="rpsB"
                     /locus_tag="ACG8PO_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFQ0207419.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            3622..4101
                     /locus_tag="ACG8PO_002123"
     CDS             3622..4101
                     /locus_tag="ACG8PO_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFQ0207420.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            4318..5409
                     /gene="phnW"
                     /locus_tag="ACG8PO_002124"
     CDS             4318..5409
                     /gene="phnW"
                     /locus_tag="ACG8PO_002124"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047304 - 2-aminoethylphosphonate-pyruvate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HFQ0207421.1"
                     /translation="MYKLLTPGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSGSFGVESVLSSVIRPEDTVLICSNGAYGQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQEKDLL
                     FIVDAMSSFGGIPIRVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            5402..6187
                     /gene="phnX"
                     /locus_tag="ACG8PO_002125"
     CDS             5402..6187
                     /gene="phnX"
                     /locus_tag="ACG8PO_002125"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050194 - phosphonoacetaldehyde hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HFQ0207422.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIREP
                     MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEHYLFKDLVKHSTLKP
                     DTLETIERLRNAGINIGSTTGFTAPMMTIVAETAKKEGYSPDFMATSEDVEGYGRPFP
                     YMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEGSSLMGISYDEYQKL
                     EMGKKDRLKQSVALRFQQAGADICVNNLKELTEYILYADKNPE"
     gene            complement(6533..8224)
                     /gene="argS"
                     /locus_tag="ACG8PO_002126"
     CDS             complement(6533..8224)
                     /gene="argS"
                     /locus_tag="ACG8PO_002126"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFQ0207423.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDEEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMRHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(8646..9593)
                     /gene="arcC"
                     /locus_tag="ACG8PO_002127"
     CDS             complement(8646..9593)
                     /gene="arcC"
                     /locus_tag="ACG8PO_002127"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFQ0207424.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(9708..10727)
                     /gene="argF"
                     /locus_tag="ACG8PO_002128"
     CDS             complement(9708..10727)
                     /gene="argF"
                     /locus_tag="ACG8PO_002128"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFQ0207425.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(10818..12047)
                     /gene="arcA"
                     /locus_tag="ACG8PO_002129"
     CDS             complement(10818..12047)
                     /gene="arcA"
                     /locus_tag="ACG8PO_002129"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016990 - arginine deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0019546 - arginine deiminase pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HFQ0207426.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
BASE COUNT         3349 a         2537 c         2192 g         4125 t
ORIGIN      
        1 ctttttttaa tttttaaata aaagaaatat aaataaaaac agaggaagcg gaatgatccg
       61 cccctctgtt tttatgattt tattaaactt ccaataattc tttttcttta tcagcagcga
      121 tcttatctac ttctttaatg ctgttatctg tcaatgtttg tacgtctttt tctaatccgc
      181 gcaaatcatc ttcggtaata tcaccatttt tttgttgttt tttatattca tcgatcgcat
      241 cacgacggat gttacgaatc gcgatttttg cattttctgc ttcttttttc acatctttag
      301 ctaattcttt tctgcgttct tctgtcagtt gcgggataac taagcgaatg acatttccat
      361 cattggttgg actgattccg atatcacttg ccataatagc tttttcaaca tcttgaagaa
      421 tgctcttgtc aaaaggtgtg atcattaaaa cacgtgcttc tggaatttgg attgatgcca
      481 tttggttcaa tggtgttggc gcaccgtagt agttcacagt aatacggtcc aaaaggctag
      541 cgttcgcacg gcctgcgcgg atttgcccaa gttcacgttg aagattttgt gctgcttttt
      601 tcattttttc ttttgcttct gtcataattg catctgccat ttttatttcc cccttacagt
      661 tgttccgata ttttcgccta agattgcacg gcgaatattt ccgtgttcat ttaaattgaa
      721 gacaagcagt ggaatatcat tatccatgct tagtgaactt gcagttgaat ccatgacttg
      781 taatcctttg gcaataactt ccagatgagt cagttcttca aattttactg catttgcatc
      841 tactttcgga tcagcagaat aaacaccgtc tacattgttt ttagccatca ggataacatc
      901 tgcgccgatt tctgccgctc gtaatgctgc tgttgtgtct gtcgagaagt acgggttgcc
      961 ggtaccgcct gcaaagataa cgatccgtcc tttttctaaa tgacgttctg ctttacggcg
     1021 aatatatggc tctgcgattt gacgcatttc aatcgaagtt tgcacgcgtg tcggtacacc
     1081 cacgttttcc aacgtatcct gtaaagctaa ggcattcata actgtagcta acatacccat
     1141 gtaatcagct tgagcacgtt ccatgcccat ttgtgctccg atttgtccgc gccagatatt
     1201 tccgccaccc acaacgattg ccatttcaac gcccagctcg tgaacttctt tgatttcttg
     1261 gatgatttct ttgattactg gtggtttgat tccaaatcct gcatccccag ctaaagcttc
     1321 tccactcaat tttaagacta cacgctgata tttcggtgtg accatgttca ttcctccaat
     1381 attttctatt gccattttac catacttaga tgatttcgac actctaagac tggtttttct
     1441 tgttttgctt aaaaaaaggg agcgcacaaa ccgtgcgttc ccgtctgtta ttcaacagaa
     1501 ttattttttc acttggctca taacttcttc tacaaagtta tcttcgcgtt tttcgatacc
     1561 ttcgccaact tcgaaacgta cgaatgtttt aacagttgcg ccttttgatg caacgtattt
     1621 ttcaacagtc atatcaggat ctttaacgaa tggttggtcc accaaagcga tttctgcttt
     1681 gaatttcttc aagcgtcctt ctaccatttt ttcaacgata ttcgctggtt tgccttcgtt
     1741 caatgcttgt tctgtaagaa cagttttttc atgttctaat tcagcttctg gaatttgtga
     1801 ttcgtttaca taacgaggat tgatagctgc tacgtgcata gctacgtctc ttgcaactgt
     1861 ttcgtcagtt gttccatcta gaacagtcaa tacggcaata cgtccgccca tatgcaagta
     1921 tccaccaaat gcagcattgt cgtctttttc tacaacttca aagcgacgga agctgatttt
     1981 ttctccgata acttgagttg cttcgatcaa gtcagattca attgtacctt tatctgtttt
     2041 gattttcatt gcagcttcca tgtctgctgg tttgttttca gcaacaagtt ctgcaatttc
     2101 ttttactaaa tcttggaaca tttcgttttt agaaacaaag tctgtttctg agtttacttc
     2161 aacgattgct gcagtgttac ctttcacagc aactgaagcc aatccttcag ctgcaatgcg
     2221 gtcatttttc ttcgcagcct ttgccattcc tttttcacgt aaaagatcaa ctgctttttc
     2281 catgtcgcct tctacttcaa ctaatgcttt tttcgcatcc atcattccta cgccagtcat
     2341 ttcgcgtagt tcttttacca ttttagctgt aacgtctgcc atgtttaact cctcctgtgt
     2401 gtttttcttt ttaaaaaaag ctgcttcaaa ggagagtgag gcttgtcgcc cagcctttga
     2461 aacagctcca tcattcaaaa ttattctgct gaagaattgt cgccttcaac aacatctacg
     2521 atttcttcga ttgatgttgc tgtgttttct tctgtgaaat cttcttcaac tacttgatct
     2581 tcaccttggt ttccttcgat gaaagcatca gccatttttg aagtaatcaa tttaacggca
     2641 cgaattgcat cgtcgtttga agggattact acgtcgattt catctggatc gcagtttgta
     2701 tcaaccatag ctacgattgg gatatttaat ttgtgtgctt cttgaacagc aatacgttct
     2761 ttacgtgggt caacgatgta cattacatct gggattcttg gcatatcagc gataccgcct
     2821 aagaattttt caagacgttc acgttctttg ttcaaaccag caacttcttt tttaggtaga
     2881 acttcaaaag ttccatcttc ttccattgca ttgatttgtt tcaaacggct gatacgtttt
     2941 tggatagtat cccagtttgt caatgttccg cctaaccaac gatggttaac gaagtattgt
     3001 cctgcacgag ttgcttcttc tttgattgct tcttgtgctt gtttttttgt acctacgaat
     3061 aatgctacgc cgccttcttc agcaacgctc ttcatgtaat cataagctgc atctactaat
     3121 ttaactgttt tttgcaagtc aatgatgtag attccgtttc tttctgtgaa gatatatttc
     3181 ttcatttttg gattccagcg acgagtttgg tgaccaaagt gtacgccggc ttctagtaat
     3241 tgtttcatag aaattactgc catgtttgtt tcctccgatt tggttttatt tttcctcttc
     3301 ttatcttcta ccttgccggc aaactttttc aagcacctac cgacaatcca ataagaatgt
     3361 ggatttgctg atgcataaag caccctcagc tattatacag acaaaagatc cgtatcgcaa
     3421 gagcaaatat acttttctgc tcaacattca atagtttacc tgaaagataa aaagaattca
     3481 agaaaaacag tagatttctc tttgtttctt tattatcctt tttccataaa ataattttag
     3541 gtataaaaga cgaatattat tgggcaatcc ttcttttttt tgttatgata catgtgaaat
     3601 ctttcgtaaa ggagaaagaa aatgcgcaaa gctcaaagac atttgctgat taaacaagtc
     3661 attgaagaat tcccgatccg tacgcaagaa gaactattaa acaaacttgg gcattatggc
     3721 gttaccgcca cgcaggcaac gatttctcga gatatccgtg atctaaaaat cgtgaaggca
     3781 cctgatgaaa atggcgtttc ccgcttcgta ttgtttcgcg aaaaaggaac tcctgagact
     3841 aggactgagg aagaggatcg gctggttcaa atgattgaag acattgtatt gaaagtcgaa
     3901 cgagtccact ttttgacgat cgtcaatacc ttgccagaca atgctcattt atttgcatct
     3961 gttttagatg agatcaaacc tccctctatt gtcagtacaa ttgctggatt cgatacgatc
     4021 atggtcatct cggaaaatga ggaggacgca aaacgagtag aagaatttct tcaccatcca
     4081 actgaagtat cactttttta atcagagcac gtattcctaa tatggaatgc gtgttttttt
     4141 attttcaata ttttccttat taaataaacc tttacactct ttacaaaaat acatcatttt
     4201 ttattcaatt ttttcaacac cttgattgaa tcacaataat catcatttat attggatgtt
     4261 aacaagagca gcttcttgct ctttgtgtat ttcaaaaaag aaatgaaagg aaatcatatg
     4321 tataaattat taactcctgg accattgacg acttctgaaa cagtgaaaaa tgctatgctg
     4381 atcgatcatt gtacttggga tcaagaatac aaagaaatta cacaatggat aagaaaaaaa
     4441 ttattggatc ttgctcaagt atcggaagga atatacacaa cggtcttaat gcaaggtagc
     4501 ggctcctttg gggtagaatc ggtattaagt tctgtgatca ggccagaaga tactgtactg
     4561 atttgttcaa acggcgcata tggacaacga atgatcaaca tgtgcgaatg tctgaaactg
     4621 aactataccg tttatgaggt atcggaacat caaatcctcc aagctcatgt gattacacag
     4681 ctgattgaag agaatccctc tatctccgca ctagcaatgg tccatagcga gactacttcc
     4741 ggtattttga ataatttgga agagattgct gctgtcacac aagaaaaaga tcttttgttt
     4801 atagtggacg cgatgtcaag ctttggtggg ataccgattc gtgtagccga actagggatt
     4861 gattttttga tttccagctc aaacaaatgt attcaaggtg ttccaggctt ttcttttgta
     4921 cttgctaata aagcggaact agaaaagcga aaaaatgtga gtcgaagttt gtcacttgat
     4981 ctatatgacc agtaccgcgt gatggaaaac gagaatggca aatggcgttt cacttcccct
     5041 acccatacgg ttttggcttt tcaacaagcg ctgaaggaac tagaggcaga aggaggaatc
     5101 gaaactcgat attcacgtta tctcgccaat aatcatgtac tacgacaaaa aatgacagag
     5161 atggggttcc agcctttcat tcaagaatat caggggccat tcatcacctc ttttctttac
     5221 cctaaggact ggcgatttga ttttaatcat ttttatcagt atttaaagca aaacggattt
     5281 gtgatctatc ccgggaaaat cagcgatcat gaatgttttc gtatcggaaa tattggcgag
     5341 atctatccgg aagatatttt ggaactgaca gcaattatca aagactataa ggagaacgtt
     5401 aatgattaaa actgtaattt ttgattgggc aggaacaact gtggactttg gctgtatggc
     5461 tcctgtccac gcttttagaa atgcttttct agaaaaagga atccaactga ctgacaaaga
     5521 aatccgcgaa ccgatgggaa aattaaaatg ggatcatatc caacagcttc tttccctccc
     5581 ttctgtcaaa aaacaatgga tagctattta tggacaacta cctcaggaaa aagaggtgga
     5641 ggaattgtat caacaatttg aacattatct gtttaaagat ttggtcaagc attctacctt
     5701 gaaacccgac acattggaaa cgatcgaacg gttgagaaac gctgggatca atattggttc
     5761 cacgactgga tttactgctc ctatgatgac aatcgtagca gaaaccgcaa aaaaagaagg
     5821 gtattcacca gacttcatgg caacttctga agacgtcgaa gggtatggtc gaccgtttcc
     5881 ttatatgatc cataaaaaca tgcagtactt caaaaacaag tcggttgatg aagtaatcaa
     5941 agtcggagat acggtctcag atatccagga agggaaaaat gccggagtgt tgacagtagg
     6001 tgtaattgaa ggtagttcgc taatggggat ttcttatgat gagtaccaaa aattagagat
     6061 ggggaaaaaa gatcgtttaa aacagtctgt tgctcttaga ttccaacaag ctggcgctga
     6121 tatttgtgta aacaatttaa aagagttaac agaatacatt ttgtacgctg acaagaatcc
     6181 agaatgactt ttatacaaaa tcagaggtcg tgaaaaagct gtactgcatc aagtaataag
     6241 aacagccaaa caaaaatagc tcctcatatt tttcgtttgg ttaggcttat taccgcagat
     6301 gcaagctttt gaacaccgtt tattcgaaaa aaaaagggag ctgtgacacg acttttgtca
     6361 cagtcccctt atttacaaac aaacagtgtt taagagacaa cctctccact aaacggtgtt
     6421 cattcagctg actctcggta tttgctcaaa aaacgataaa atttgaaaag ctattttctc
     6481 ttttgtttct ctaatactcg gatcagaacg ctgatttcac attctctttt tcttacatct
     6541 tatctggtgc atgaagtcct agcaaacgaa ggtcttcttt caataataca gtcactgcat
     6601 aaactagagc taagcgtgct tctttttgtt catcatcagc taaaatcttc gtatgagcat
     6661 agtatttgtt gaatgcttgt gctaatttga ttgcatgttt cgcaatcact gacggttcgt
     6721 atttttcccc agcagacaag accgtttcag ggtatttttg aactaattta accacttccc
     6781 agctggtatc gtcattcaat gcatagtctg ctgtttctga agggacaaat cctgcttttt
     6841 ccaacaggct gactgcacga gcatgtgtat attgcacata aggtccagtt tctccttcaa
     6901 atcgaacaac ttcttccagg ttaaagtcaa atgtattcag tcgatcgttt ttcaaatcgt
     6961 ggaaaatgac tgccccaacc ccaacttgtt tagctacagc atctttgttt tccaaatcag
     7021 ggtttttctc gctgatttgt tctttagcag attggattgc ttcatttaat acttcttcta
     7081 ataagacgat ctttccttta cgagttgata attttttacc accttgcgtg atcaaaccaa
     7141 atggaatatg gcgcatatcg tcagaccagt caaagcccat ttccttcaag acagctttca
     7201 actgtttaaa atgatagctt tgttcattcc ctactacata tagagattgt ttgaagtcgt
     7261 aagtacgttt acggtacaat gcagcagcta agtcacgtgt aatgtatagt gtcgcaccgt
     7321 ctgacttctt aatcaacgct ggattcaggt cataagcaga taggtcaacg atctctgcac
     7381 ctttatcctc gttcagcaaa tgtttttctt ctaaaagttt gacgatttca tccatcttgt
     7441 cattgtagaa agcttcaccg ttcaacgaat cgaaacgtac ttccagcaga tcatagattt
     7501 tattgaattc tttcatcgat tcatcacgga accattgcca taattgaata gcctcttcat
     7561 cgccttcttc cagacgtttg aaccaagcac gagcttcttc atttaattct ggttctgttt
     7621 cagctacttc atggaattgg acatataaac gaagcaattc attgattggt tctgctttaa
     7681 cagcttcttc tgtaccccat tttttgtaag cgacgatcag ttttccaaac tgtgttcccc
     7741 agtcacctaa atggttgatg cggattggct gataaccgat tttttccata atgaaaccga
     7801 tcgaattacc gataactgtc gaacgcaaat gtcccattga gattggtttg gcaatattag
     7861 gagaagacat gtcgattggt actgttcctt gattcccgat attgctatcc ccataatgtt
     7921 ctttttcttt cactactgtt tgcaaaactt ttttgctgat aagttcttta ttcatgaaaa
     7981 agttcaagta aggtccgaca acttcgattt tttcaaagtt ggcagaatcg attttttctg
     8041 ctaaatctgc agcgatttgc tgtggcgctt ttcggtaaac ttttgctaat gaaaaggctg
     8101 gaaatgcaac atctccgtgt tcagctgatt ttggattttc tagtaattgt tcgacttgtt
     8161 ccagtgttaa atcatctttg acagcgtcat agacagcttt tgcgacaaga tctttattat
     8221 tcatctttat tcctcctaaa gtgctccctt gattgttaaa caaaaaaaat ctctagcgac
     8281 ggcatactta gtatgccatc actagagacg agatttcccg cggtaccact ccaattgtcc
     8341 taaaaaagga ccagctccat ctgatatcgg tagacgaccg tctgtttaaa acagaattct
     8401 ccaagtgcgc ttcgttgtat cttcttcatt cggcttccac cacttccgaa ttcgctagcc
     8461 aagaatgata caagtactct cttgttcaac aaatcttagt cttattaaac tttcttgaat
     8521 tatatcagaa aaaaaaacga aaggctagtc gtttctgata aaaaaacaat ccagatggcc
     8581 taaaggagaa gaaataccac ctggatagct ataaatcatt tcgtaaatac gaaagactta
     8641 acaaattaat cagctacgat aatcgttcct tcttcagaag ctaaaagatt ctcgatattt
     8701 tctaaagaag tgatgattgc cttagcgttt ggttttgctt caacaaatgc aatagctgcc
     8761 tctactttcg gaagcatgct tcctggagca aattgctttt catcaatgta ttgtttcatt
     8821 tctgaaacag taacagtttc taatttcttt tgatctggtt tttggtaatt gacatacaca
     8881 ttgtctactc ctgtcaatac gatcagcaaa tccgcatcga tgatttcggc taatttttct
     8941 gaagcaaaat ctttatcgat tacggcttct cgtccttcta atccagtagc tgtttctaca
     9001 acaggaatcc caccgccacc aacagagact gtgataactc cttgatcgac cagcgtttcg
     9061 atgacacgtg cttctttaat cgagatgggt ttaggggaag cgactacttt tctccatcca
     9121 cgtccagcat cttctttgaa tgtcacagtg gagtcagcat tcatttgttc tttggcttct
     9181 tcttccgtgt agaatggacc gactggttta cttggatttt tgaatgaagg atcattttca
     9241 tccacaataa cttgagtgac taaagaaacc acatctttat cgatcccttt ttccttcagt
     9301 acttcgccca tggcattttg taaccagtaa ccgatagatc cttctgtcat tgcaacacat
     9361 gtatctaaag gcattgccgg tgttttctca gaatcagcag cttgctgctg aatcaatagg
     9421 ttaccgactt gcggaccatt tccgtgggag atgatcaatt catctccttg ttcgataaat
     9481 ttcactaaat actttgctgt ttccattaac gcttcttgtt gggcttttgc acttgcatcc
     9541 gtagacaaga ttgcattacc ccctaaagcg acaactactt ttcgtttggc catttcattc
     9601 gtcctttctt cagttctacc gaattttcta gataacaaaa cgagccagcg ttttgtgatt
     9661 ttccctcaat gctgactcgt tttttcgtga gatgaatgtg gttatgctta aacgcgaggg
     9721 ataaacaagt tacctaatgt tgctgccatg atcgctttga ttgagtgcat acgattttct
     9781 gcttggtcaa attgacgagc atatttgctg cggaagactt catctgttac ttccatttca
     9841 gtgatgccaa agcgttcttt catttgttca ccgtaaacag tgtttgtatc atggaaagct
     9901 ggcaagcagt gcaagaagat caaacgatct gtgttgtgtg ttttttctac catttccata
     9961 ttgatttggt aaggtttcaa caatttgata cgttcttcga atttatcttc ttcacccatt
    10021 gatacccata catcagaata aaggacgtct gctccgtcta cacctttatc aacatcgtcg
    10081 gtaatcatca attgtgcacc ggatttttca gcaaagcctt ctgccatttt gacgatttct
    10141 tcttcaggtt gtaattcttt tggtgcaacg atacgcatat tcgctcctaa aattgcacct
    10201 gtgactaata aagagttagc catattgttt cgaccatctc cgcagtaagc aacagtgata
    10261 ccttctactg ttccaaagtt ttcttggata gttaaaaagt cagcgatcat ttgtgttgga
    10321 tgccattcat cagtcaagcc attccaaact ggaacaccag agaattctgc tagttcctct
    10381 accatttttt gactgaatcc acggaattcg attccatcaa acatacgacc tagtacttta
    10441 gctgtatctt ctgtggattc ttttttacct aattggatat catttgcacc tagatattca
    10501 ggatgtgcac ctagatcgat cgctgcagta gtaaatgcag aacgagtacg tgtagatgtt
    10561 ttttcaaata aaagcgcaat attttttcct tctagatagt gatgtgggat accacgtttt
    10621 ttcaagtctt tcaagtgttc tgaaaaatcg atcaaatatt gtaattcttc ttttgtaaag
    10681 tctttttctg ctaataagct tcttccttgg aatactgatt ctttcatatt aaacattctc
    10741 ctttagattg tttttattta agattaaaga ggaaatcccg acggcagctg acagtaagac
    10801 cgccgtcagg aaacaaatta cttcaagtct tcacggacaa gtggttggct catgcagcgt
    10861 ggaccaccac ggcctcgtga caattcactt gaattgattt ctaacacttt gatgccatag
    10921 ctacgaagca attcgttcga tacataattt cggttataag tgactacaac acctggagcg
    10981 attgccaatg tgtttgatcc atcattccat tgttctcgag gtgcaacgat ctcatctcca
    11041 ttaccagttg ggatcagtac aagatcatct aatccaagag cagctttcat cgtttcgtgt
    11101 aagttatctg aatgagtaat cttgatatca tcaccatctg gttcgattgt aaagacatcc
    11161 atcttaccgt ctttactttg aattgctgga tggatagtga atttatcgtg atctaccatt
    11221 gtaaatacgg tatccaaatg catcattgca cggttgtttg gaattttgat tgctaacact
    11281 ttttcaaatc ctgaattttt tgcgaatagg tggcgtgcta atttttctaa agctttcgca
    11341 ttagttcgtt gagaaatccc aacagctact actttatcgc ttaagatcaa ttcgtctccg
    11401 ccttcgatat gatctggatt ttcacgatcc aaccatactt cgattccttt atttgcaaat
    11461 cttggatgat gtttcaaaat atattctgta aacatcgatt cacgacgacg tgcttcaaaa
    11521 gtcatcttat tgactgtcat cccatttccc attgatgcgg aagggtcgcg tgtaaagtac
    11581 aagtttggca ttgggtccat gtaaaatgga tagtcatcgt ctgctgacag atcgtataga
    11641 ctgctagaac gtacttcaat atcttttgta cgaaccccag ccatgatttt atctaccatt
    11701 tcttgcgttt ccattgatag aaggaactca tgaagaccct ctcttacagc attcgatgca
    11761 atatgagatt catccaacat tttattcaag aattgttctt tgacattgcc tgcgtcaatc
    11821 gcttctgccg ctaatttttc caagtaaaga gtctcgacgc cttcattttg caatgtcttt
    11881 gcaaagttgt catgttcttc ttgagcaatc ggaagatatg gaatatcatc gaacaacaga
    11941 cgatccataa tatctggtgt caaattttcc acttcttgtc caggcctttt cagtaaaact
    12001 gtctttaatt tccctatttc agagaaaacg tgaataggtt tatccatgtt gtgttgcctc
    12061 cttctttttg attacatgta taggatatcc tccttttgta atcactttca taattttgtc
    12121 tcattcaaaa tgagacattc ctcacattaa tattcgataa ttaaataatt aaacgttttt
    12181 ctatttttaa gcgtaattta ttc
//
LOCUS       DAZKKW010000079        12186 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.081, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000079 DAZKKW010000000
VERSION     DAZKKW010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12186
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.081"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(148..312)
                     /locus_tag="ACG8PO_002130"
     CDS             complement(148..312)
                     /locus_tag="ACG8PO_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0207427.1"
                     /translation="MKNLIAGGFLFVGGGILMNTDNFGNVIPFLGAACALIGVGMLIY
                     FVFSDDGKYG"
     gene            607..1935
                     /locus_tag="ACG8PO_002131"
     CDS             607..1935
                     /locus_tag="ACG8PO_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HFQ0207428.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            2137..2970
                     /locus_tag="ACG8PO_002132"
     CDS             2137..2970
                     /locus_tag="ACG8PO_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HFQ0207429.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            2986..3723
                     /locus_tag="ACG8PO_002133"
     CDS             2986..3723
                     /locus_tag="ACG8PO_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP thioesterase domain-containing protein"
                     /protein_id="HFQ0207430.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(3878..4846)
                     /locus_tag="ACG8PO_002134"
     CDS             complement(3878..4846)
                     /locus_tag="ACG8PO_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFQ0207431.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            5078..5911
                     /locus_tag="ACG8PO_002135"
     CDS             5078..5911
                     /locus_tag="ACG8PO_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HFQ0207432.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            5945..6784
                     /locus_tag="ACG8PO_002136"
     CDS             5945..6784
                     /locus_tag="ACG8PO_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFQ0207433.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            6810..7370
                     /locus_tag="ACG8PO_002137"
     CDS             6810..7370
                     /locus_tag="ACG8PO_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFQ0207434.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            7569..9290
                     /locus_tag="ACG8PO_002138"
     CDS             7569..9290
                     /locus_tag="ACG8PO_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HFQ0207435.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            9455..10597
                     /locus_tag="ACG8PO_002139"
     CDS             9455..10597
                     /locus_tag="ACG8PO_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HFQ0207436.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            10613..11824
                     /gene="thiI"
                     /locus_tag="ACG8PO_002140"
     CDS             10613..11824
                     /gene="thiI"
                     /locus_tag="ACG8PO_002140"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HFQ0207437.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
BASE COUNT         4153 a         2044 c         2531 g         3458 t
ORIGIN      
        1 actgccggag taaataaagc ttatgcaaag aaaaagaaat ccaatcactt gggtttcttt
       61 ttcttttatt attatgatga atcaagcttt ctgtttttgt cacgtatgtt gtaatcttta
      121 cgaaagagta gagttggcta ctcataatca accatacttc ccatcatcgc taaatacgaa
      181 ataaatcagc attcctacac caatcaaagc acaagctgca ccaagaaaag ggatgacatt
      241 cccaaagtta tctgtattca taagtatgcc accacccaca aacaaaaagc cgccagcaat
      301 caggtttttc attttattca tctcctttct tacgttatag tatttccttc tttcaatgag
      361 tattttgaaa gctatcttat tgtatgatgt tcttctaggc ctcttcttta ttatacaggc
      421 cattttcgtt atctcataga aatattcctt tttatctgaa aactattgta taccgtatga
      481 catttttctt catgaagttg taagtatatt ctcattcaaa tgaaaatttc atcatagaag
      541 agattgtatt tctaaattct taagagtaaa atacgaagta taacttggat ttggaaggat
      601 gattggatga aagctattac gcctgatatg acaacaaaat ttaacaaaca gtttcaagca
      661 aatatcaaac agcaggcatt gcagcgggca gttgtgaaga acggaattac tgcttctgct
      721 gaaaatgttt cagcacatgt ggagaataca ccggtatttt ctatagattt ggctacgggg
      781 aaagtggcta atcaaatgca atcggggcgt tgctggatgt ttgctgcact gaacacattc
      841 cgtcataaga tgctgaatca attccattta aaagagtttg aattatcaca aaattacact
      901 tttttctggg ataaatatga aaaggcaaat tatttctatg aaaacatcat tgcaacagga
      961 aaagaggcaa tcgatagccg gaaagtagct ttcttactcg ctactcctca acaagatggc
     1021 ggccaatggg atatgatcgt ttcattattc caaaaatatg gcgttgtgcc taaatcggtg
     1081 atgccggaaa gtgcgaatag ttcaaattct cgagatttga ataactactt gaacaaatta
     1141 ctgcgtaaag atgcagtagt gttacggaaa atggttgctc aaggagaaac attagacgct
     1201 attgaagaaa agaaagaaga aatgctagaa agcatttata acttcttagc tatctcatta
     1261 ggaacacctc caaaagaatt cgattttgag taccgcgatg aagaaaagaa ctatcatctt
     1321 gaccgaggac tgacaccaca aattttttat gataaataca tcggtgtaaa attagatgat
     1381 tatgttagcg tgatcaatgc accaacaaaa gacaaaccat tcaatcgttc ttatacagta
     1441 gagatgctag gaaatgtcgt tggcgggaaa gaagtcaaat atctgaatgt tgatatggaa
     1501 actttcaaaa aattagcgat tgctcagctt gaagaaggag aatctgtttg gtttggttgt
     1561 gatgtaggtc aatcttctac tagaactagt ggcatcatgg ccttagatgc ttattcaatg
     1621 gatgatctat ttgatacgga ttttacaatg actaaagctg aacgtttaga ttttggagaa
     1681 agtctgatga cccatgcgat ggtattgacc ggtgttgact tagtagacgg gcaatcaaca
     1741 aaatggaaag tcgaaaatag ctggggcgaa aaagttggaa agaatggctt ctttgttatg
     1801 agtgatgcct ggatggacga atatacgtac caaatcgttg taagaaaaga tttattgacg
     1861 aaagagcaat taaatgcgtt caatgaagaa ccaattgttc tatctccgtg ggatccaatg
     1921 ggtgctttag catagacgag tatttcaatg aagagtatgt agtaaagata aaaagagctg
     1981 gcttaagcca gctcttttta tctttaaagt ccattccctg ttttttatgg tccctgtgct
     2041 ataattctaa ataaaggata aggattggaa ataatccttt tatcattaaa aatatgatga
     2101 cgaagtagaa taaaattggg agtagggggg aaagtcatgg atatccaaac tgttttatct
     2161 gatctgaaat taaatggtaa agtgattcca gttgttggtg gcgatgttaa tcagacctat
     2221 cgtataaaaa ccgaacatcg tgcttatttt ttaaaaatcc atccgaatgt aaaaaaagga
     2281 ttttttgaag cagaagtaga cggattaaaa gaactgtcag cattcgtacg tgtaccggat
     2341 acgtatatgc taggtgaaac aagtgaaggc gcttatttgt taatggaatg gatcgagccg
     2401 ggaaaagggg atcaacgaga tcttgctgca gctttagcta atctccatca gcaaacagcg
     2461 ccacaatttg gttttcgtaa agataactat ttgggtacac ttgttcagaa aaattctttt
     2521 gaagaagatt ggtggacttt tttctttaaa gatcggcttg aatcccagat ttctttggca
     2581 gaagaaacga atcgatggaa tgtacagcgt caagaaaagt atctgcgatt taaagaacgt
     2641 gtattgaaat cggttgaacc aaaaaagatc actccacgcc tcttgcacgg agacctgtgg
     2701 agtggtaatg tgttctttga ccagcaaggg catccagttt ttgtcgaccc agctgtttct
     2761 tatgggaatc gggaacaaga tattgcaatg agccagttat tcggtggttt ccgaccggaa
     2821 tttttagatg cttatcaaac tatatttcca ttagaaaagg gatggaaaga ccgactgccg
     2881 atttaccagt tatattatct tttggcacat ttgaatatgt ttggagaaag ttacggttcc
     2941 caagtagatc aattactaga aaatttttaa gaggagggcg tgtttttggc aaaaaaatat
     3001 accaaaaagc atgaagtttc atattatgag tgtgacatca accaaacgat gacatttcct
     3061 tcgatgttag gagtagttat caaaacgtct gaagatcaaa gcgaccagtt gggaagaggc
     3121 agtgaattcg tcaatagctt tggacttact tgggtcatca cgaattattc catggagatt
     3181 aatcgtctcc ctagagtcgg tgaagtaatc tcagtaacga ctcaagccat ggagtacaat
     3241 aagtttttct gttatcggaa cttctggatc catgatgaag atgggaaaga attagtcaaa
     3301 atagaatctg ttttcgtatt gatggatttt gtgaatcgaa aaatgagcag tgttaatgaa
     3361 gagatcattg ctccttttga gagtgagaag atcaagaaaa tcaagcgtca ggaaaaaatt
     3421 gaaaagattg attcgggctt catgttgcca tatcgtgttc gtttttatga tatcgatagt
     3481 aatcagcatg ttaataatgc gatgtatttt aattggatca ttgacgtatt aggatatgac
     3541 tttttgacta cgcatgttcc agaaaaagta attattcgat ttgataaaga agtagagtac
     3601 gggaatgaga tcgaaagcca ttttgaacaa atccctatcg aaaatggtgt caaaacaaaa
     3661 catgaaatcc gaatgcaaga acagctgtat tgtgaagcaa atattatatg gaaaaataaa
     3721 taaaaaagag gttgtgacag aagggggcaa cttcaagaaa taagaagaaa tttacgaaaa
     3781 ttgtttctca aatttttgta aattttcgac ttagttttcg aagaagctat ttttgtcaca
     3841 gcctctcttt ttaagtttag ctattagata atgatgttta gttttggata aacgaaatca
     3901 attcttcacc cgacaaacca tagtttacag caatcaatag ttttaaacgt gctttttgac
     3961 tattaatact gttacagaaa ataatcccca ttttttctag ttcttttccc ccgccttcat
     4021 aatcataaac aggctctgcg attccattga aacaacgaga aacaagtact acagggattt
     4081 tttggacaag aagcttttcc aaagcagcta aggcttgtgg agggagattc cccgcaccta
     4141 aagcttcgat taccaaaccg tctactttcg tatgggcgat ggcttccaat aagtcccctt
     4201 gcatcccagc atatgcttta acgatcggga tagtcccatc tacagcggaa atgtccagtc
     4261 gctttgtttc taaaagttcc tgcaagaata aaattcgatt cttagttact aagccgatgg
     4321 gacccaatgt aggtgttcgg aaagtagcaa catttgtggt atgggtcttt gtcacgtagc
     4381 gtgcagaatg gatctcatcg ttcatgacca ccaaaacccc cttatctagc gcttcttcac
     4441 aacttgcgac acgaatcgca ctttcaaaat tataaaggcc gtcacttcca agttcattac
     4501 ttgaacgcat tgccccagtc aaaacaattg gtaatttgtt gccaatcgtt gtatcaagaa
     4561 aaaaagcagt ttcttctaat gtatctgtac cgtgtgtgat caccactccg gatatacctg
     4621 atgcataagc ggacaagata cgttctttta ataccaacat gtgagaaggc atgatgtgtg
     4681 gtgagggaag attgaagata tcttcaacta ataattctac atgcttgggg attgtgattt
     4741 ctgcatctaa taatgggttt gccacatcag gactaacacc gcccgtgagc atgttttctt
     4801 tcatggcgat cgttccgccg gtgtgtagta caagtatttt tttcatttta cagacttcct
     4861 tacgaataat tttgcatagc cgttaaatct tattgtatac tatataaaag agttttgcta
     4921 tcagtgaaga ataacgaata gcctaactta tcataaaaaa gctgacctta ttaaaatgga
     4981 aaagactcaa ccttgacctc ctaatacaat ggagtatctg gttgagtttc ttttacaaga
     5041 aagcagccaa ggaaatcaat cacgaaggtg gccggaaatg atcagaatcg gattaacatc
     5101 gtttaaagaa catgacaaat taactggtaa aaagaataac actctttatg aatatgcagg
     5161 gtttctaccc ttagtggaat tagatactgc ctattatgga attccccgaa agtcctcagt
     5221 tgagaattgg ataagtgaag tacctgaatc atttcgcttt gtagtcaaag tctatcaagg
     5281 aatcagcggt caaggagact ggaagcctta ttatcagacc gaagaagaga tgattgcgca
     5341 ttttttagat gttatgtcgc caatggtaga tgctggaaag atgttttgct ttttagtcca
     5401 attcccctca tcattcaaat gtacgaaaga aaatgtccag tacttgcgca aacttagaaa
     5461 gtggtttgag ggttatcctg tttctataga attgcgggat tattcttggt acagcgatga
     5521 atttcttgaa cgtacaagaa actttatgtc tagagccgct ttttctttag ccatcatcga
     5581 tgagccacag ttgctgaata caacgattcc ttttgatcca tttgtcacca atccgaactt
     5641 tgctcttttt cgattccatg gtaggaatgc gcaaggatgg caagcaaatg ataaagagtg
     5701 gcgtaaaaaa agaacgttat atcgttattc agaaaaagaa ctgcaagaac tagcacatga
     5761 agtcaaagta gttgattcta agacaaaaga ggtaggtgtg atttttaata ataattctgg
     5821 tggtgatgcg gctgaaaatg cattagcact gcaaaaaata ttaaatatca agccagatgg
     5881 actcaatcca acccaaatgg atctgtttta agggcttatt ttattcaaga aaggggcgaa
     5941 gtctatgatc aaagctattt tttttgatat tgacggaaca ttggtcacca atcgttcaaa
     6001 agcgttggaa tcaacaaaac aagcaattga atatgctcgg aaaaacggca ttttatgcgg
     6061 tgtagcaacg gggcgcagcc cagtaaagat caaagaaatc attgatgaat tggaattaga
     6121 tatgtatgtc gtgtataatg gtcaactggt ttttactgca gatcgcacga ttatcgacca
     6181 cccttttgaa caaaaagtat tggaacatat tgttgaattt gcagatgaga accatcgaca
     6241 aatcgttttt ggtgcgagaa atcggttaga tggatcaacg acgatgctat taggacaatc
     6301 gatttttatc aagcgactag tcagcttttt accgcgtaaa ttcccggtac gtctgatgaa
     6361 aaaaatcctc caggttttta gccctcaccg acaaaaggat cgttatgaaa cgttagagat
     6421 attaaaagaa ccaatttatc aatgtatatt gctcagtccg gagtcagaac agaaaaagtt
     6481 agaacatcgc tttcccgagt gtacgtttca gcgttcaaat acgtatactg tagatatcat
     6541 cccaaaaggt ggttcaaaat tactaggcat ccaagctttc gcaaatgctg tcgaaattga
     6601 aatggaagaa attatggcat tcggcgatca ttacaatgat atcgagatgc tgaaaggtgt
     6661 aggaattgga gtggccatgg ggaatgctca gatcgaagta aaacatgctg cagactttgt
     6721 gactcaatca aatgaagaag atgggatata cgaaggactg aaacacttcg gtctgattta
     6781 ttagtcaatg aaagaaggaa aaaatcagta tggaagcaga gacacctttt caaaaagtag
     6841 aaacattcca caaaactttt gatccaagaa gaccagagat acccacggct tttacagaaa
     6901 atgaagctca tacacgtgct gcgttcaaaa tagaagaact tgttgaattt gttgcagcta
     6961 cttcacctaa taaagaacaa ctgcatgaag ctgttatgct gctgcacgaa gcattagata
     7021 aagcagaaga aaaaatactt ggtaaaacac agtggggaag tgatccgctg gttgaacaag
     7081 tagatgcact gacagatatc ctttacttta cttatggttc tttttcatta ctaggaatag
     7141 tgcctgatcg aatattcgaa atcgtccatc aggcaaatat ggggaaatta tttccagatg
     7201 gaaaacctca ttatgaccct gtaacaaata aagtgttgaa acctgaaaac tggcagatgg
     7261 aatatgctcc agaacctaaa atcgcggcag aaatccaacg tcagattcaa gaagcgattg
     7321 ctaagaagaa atccgctgat ataggcatgt gcacaaatga aaaagagtga tcagagtttc
     7381 actatttttg ttatctttat acgatggaaa aacaaaagat tttcgtttaa gttagtgaat
     7441 gcatgaaaat agtatgctat aatctagaat agatgtcttt tgcacgagag acgtattatt
     7501 tggatttatg ccaagcagaa cgccatctgt tgcataaaag atgcatactg aaaacggagg
     7561 aaccataaat gggaaataat gttatcatcg gaatcgtcat tgcaatcatt atagtcgcca
     7621 ttgtcgtctt tttggttagt tacttgatga agaaaaaaaa ccaagatcga ttggatgcct
     7681 tagaaaaacg caaggaagaa ttatttgact tgccagtgat tgaagaagtc gatgatgtta
     7741 aaaaaatgca tctggttggg caaagccaaa atacctttcg tgaatggagt caaaaatgga
     7801 atgacatatc tacttcctcg ttcgctgaac tagaaagtca aattttcgaa gtggaaaacc
     7861 tcaatgaaag ttttcggttc ttaaaaggaa aaaaagcagt tgttgaagca gaagctacaa
     7921 tggaccaaat ggagaaacaa gtgacggaaa tccgtgaagg tctaaaggaa ttgcgggaaa
     7981 cggaagaacg caactcacta gctgttcagc aggcacttga cgtctatgaa gaactgaaag
     8041 aaaaagtcag agaagacgag catgaatacg gaccagctct ttttgaattg caaaaacaga
     8101 tcaaagtgat cgaaaaagaa tttacgcagt ttgtgacttt aaacacttct ggagaccctg
     8161 ttgaagcccg cgaggttttg gataaagcag aaaaacatac gtatgaagtg gaagctctga
     8221 tgaaaaaaat tccaccgctg tatgaggagc tccataccac tttccctgaa caacttgaag
     8281 aaatcagtga tacttacgag aaattgatgg aagaacaata tgtattccca gaagaagatc
     8341 ttgctgaaga tatcgcaaaa gtaagtcgaa gaattgaaaa ttcattagcc aatcttgaaa
     8401 aaacagaagt tgaaacggtc gaatttgaaa accgtgaaac agctgatttg attgattcgt
     8461 tatatgatat cttagaacgg gaaatggaag ctcagagata tgtaaaaact aaccaatcaa
     8521 cgattgcaga gtatatcaaa catacaacaa agaataaccg tcaattattg attgagctgg
     8581 atcacacatc tcaaagctat acattgaacc acaatgaaat tggccgtgta cgaggtttcc
     8641 aaaccgaagt ggaagaaatg gaacgtcaaa atgaacagat gattccacag atccgtcaac
     8701 acgaaattcc ttattcagaa gtgcgcacgt tctataaaac agtcttcaaa gtcttagaag
     8761 atatcgaaaa gcaacaagta gagatcgacg actcattaca tgaattgaga aaaggcgaaa
     8821 aagaagcgca agaaaagatc gatacattcg aattcaaatt gcgcagtttg aaacgttttg
     8881 tagaaaaaca acgtttgcct ggcttgccga atgattacct agaattcttt tttgttgcta
     8941 ctgaccgtat tgaagaacta agtgttgtct tgaataaaat tcgagtaaac atggaagagg
     9001 tcaatcgttt agttgctcta tgtgaagaag atctagaatt attagataag aagacacatg
     9061 atttagtaga tgcagcagca ttaacggagc aaatgctcca atatgcaaat cgttatcgtc
     9121 atacacacga agaagtgaga gaagcaattg ataaaagcta ctatttattc aataaagaat
     9181 atcactatca agaagcatta gacgaaatcg gtacagcatt agagcgagta gaacctggtg
     9241 ctttcaaacg catcgaagat ttttatttta atcatccaga cttagtataa agcaaaaaag
     9301 ttagtaaatc agcaagagca agacggatta ttgtcttgtt cttgtctttt tttgtatatt
     9361 tgaaaattga aacaagtgaa aatttcacct gctcttgaaa gcaccttgga caaccgttat
     9421 aatagaaaag aatttcaaaa aagaggggtt tcttatgata tattttgata acagtgcaac
     9481 aaccccaatc tttccgcaaa gcttagatgc atatgtaaaa accagtcagc ggattattgg
     9541 gaatccatca agcttgcatg atttaggcaa tcaagctaac cggttgttgc agcaagcaag
     9601 aaagcagatc gctgatttga tccatgtaga accagaagaa atctatttta cttcaggcgg
     9661 aacagaagga gataactggg tattaaaagg aacgatgatc gaaaaaagag aatatgggaa
     9721 tcatatgatc atttcaggag tcgaacatcc ggccgtcagc gagacagcag aacaattaaa
     9781 agaattagga atcgaagtaa gtatcgcccc agtggataaa cgcggatttg tacgtgtcga
     9841 cgaattgaaa gaattaataa gaaaagagac agtattagtc tctgttatgg ctgtgaataa
     9901 tgaagtagga tcgattcagc ccatccagga aatcagtgat cttttggatg attttcctaa
     9961 aattcatttc catgtagatg ctgttcaggc gattggaaaa gtaccattct cgcaatggtt
    10021 gacagaaaga gttgattttg caacattttc agctcataaa ttccatggcc cacggggaac
    10081 tggtttcatt tattggaaaa agggtcgtcg tttagcacca ttactgacag gtggagggca
    10141 agaaagaaat cagcgaagtg ggacagaaaa tgttccaggt attgtggcta tggttaaagc
    10201 tttacgttta tcattagaga taaaggaaca aaaacctgag cagacacaac gcttaaaagt
    10261 agctctaatc aatgcgttga agacgtatga gaaagtgacg attttttcag aaggaactga
    10321 ctatgcaccg catattttat gttttgctct aaaaggaatt cgcggcgaag ttctcgtcca
    10381 tgccttagaa gaaaaacaaa tatttgtttc tactactagt gcctgctcct cacgtaaaca
    10441 tatggcaagc agtacattgc acgcaatgca tgtaccaaat gatctggcta catctgctgt
    10501 tcgaatcagc ttagatgaaa acaattcgct tgcagaagtc gagcaattta tgattatttt
    10561 caagcagctt tataagaaat tttctgcaat caattaaaag aggagtgttc ttgtgaaata
    10621 cagtgaaatt atggtccgtt atggggaact ttcgacaaaa ggaaagaatc gaaaaagctt
    10681 tatcatgcaa ctagcacaaa atgtcagaca agctttgaaa gactttccag aaatcaaaat
    10741 ccatgctgat cgggaccgga tgcatctttt attgaatggc gcagacgggc agctagttat
    10801 tccaaaacta gcaaaaatat ttgggatcca aaatttctct ccaagtattc gtgtagaaaa
    10861 agatgtcgaa gttttgaaac aagctgttca agacatcatg aaagaaattt atactgggaa
    10921 agaaacattt aaaattatcg ctaaaagaag cgatcaccaa tttgaattga caagtaatga
    10981 attgaatcaa acattaggta atgctgtttt tgatattttc ccgcatatcc aagttcaaat
    11041 gcgtcaacca gatattccat tacgtgtaga gattcggcgt gatggcgctt atctatcgta
    11101 tgaaacgatc aagggagctg gaggattgcc cgtaggaaca agcggaaaag gaatgcttat
    11161 gctttctggg gggattgatt ctcctgtagc agggtacttt gccatgaagc ggggagtgga
    11221 aattgaagct gttcattttg ctagtccgcc gtatacaagt gaacaagcac tgcaaaaagc
    11281 aaaagatcta acagccaaac tagcacctta tgtgggaacg atccatttta tcgaagtgcc
    11341 atttaccgag attcaagaag aaatcaaaaa gtcagtgcca caaggatatt ggatgacaat
    11401 tactcgtcga atgatgttgc gtttgaccga tgaaattcgc cgtattcgtc atggtttagt
    11461 cattttgaat ggtgaatcat taggtcaagt ggcttcacaa actttgcaaa gtatggtggc
    11521 tatcaatgaa gtaacgaata cgccaatcat tcggcctgta gcgacaatgg ataaacttga
    11581 aatcattgaa gtagcagaac agatcgatac atttaatctt tctatccagc cttttgaaga
    11641 ttgctgtacg atttttgctc ctccacagcc aaaaacacgc cctcaactgg aaaaggtcca
    11701 tcaatatgaa gagcgtttag caatagatga aatgatccaa cgagcactag ctggcttgaa
    11761 gatggaagag atacaagcag aatcgtctaa tgaaaaaact gaagaatttt cagaattgct
    11821 ataagaggtt atgaaaatag tgttttgacc agagtattag agaaacaaaa cgaaaaaata
    11881 gctccatgta ttttaccgtt tttgaactaa tgtcgaagat tagctgattg aataccattt
    11941 atcaagaagt tatggaaaag ccggttcttt tttactaact gaaaaataaa attttattta
    12001 gagatcaagc tacagttgat aaatgatata aaaaagagaa tttcccatga aatagcgagg
    12061 ttgtgacaga agtgggcagc ttcaagaaat aagaaggaat ttacgaaaat tgcttttcaa
    12121 atttttgtga aatttcggct tatttttcga agaagttact tctggaacac cgtgtatcag
    12181 aggttg
//
LOCUS       DAZKKW010000080        11834 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.082, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000080 DAZKKW010000000
VERSION     DAZKKW010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11834
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.082"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(301..2376)
                     /gene="glyS"
                     /locus_tag="ACG8PO_002141"
     CDS             complement(301..2376)
                     /gene="glyS"
                     /locus_tag="ACG8PO_002141"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFQ0207438.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDVKTGRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     QEMIVTQANELAEKNHWVIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGELPQSTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISD
                     VISKDTDRFGIGLSEGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAELEKQSEVDPELFENEAEKELHKAVEA
                     MKKSFADQTINENYDQLVSLRPLIENYFDQTMVMAEEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
     gene            complement(2378..3295)
                     /gene="glyQ"
                     /locus_tag="ACG8PO_002142"
     CDS             complement(2378..3295)
                     /gene="glyQ"
                     /locus_tag="ACG8PO_002142"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFQ0207439.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPENMKEAE"
     gene            complement(3679..4491)
                     /gene="recO"
                     /locus_tag="ACG8PO_002143"
     CDS             complement(3679..4491)
                     /gene="recO"
                     /locus_tag="ACG8PO_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFQ0207440.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKSGGVICEKHWPMDEHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRDLIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTPEKNMPEKEIPEKEE"
     gene            complement(4634..5533)
                     /gene="era"
                     /locus_tag="ACG8PO_002144"
     CDS             complement(4634..5533)
                     /gene="era"
                     /locus_tag="ACG8PO_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFQ0207441.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            complement(5548..5943)
                     /locus_tag="ACG8PO_002145"
     CDS             complement(5548..5943)
                     /locus_tag="ACG8PO_002145"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFQ0207442.1"
                     /translation="MDSKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFGIL
                     ALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKDMA
                     AGAVLVTACFAVIIGLILFVPKIYHLLFN"
     gene            complement(5927..6403)
                     /gene="ybeY"
                     /locus_tag="ACG8PO_002146"
     CDS             complement(5927..6403)
                     /gene="ybeY"
                     /locus_tag="ACG8PO_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFQ0207443.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLKLPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISIER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            complement(6420..8612)
                     /locus_tag="ACG8PO_002147"
     CDS             complement(6420..8612)
                     /locus_tag="ACG8PO_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing metalloprotein"
                     /protein_id="HFQ0207444.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIICGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQKRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDAINKSYDEKIDKAKEDENIPKPTTEERIAALKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEEQIQYLNVTPAQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSIV
                     LMKFFQLFQTEQATYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTIILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIVYQGMSFGD
                     ATTWLMLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICKQHHGTTLMRYFYIKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKTEVEKIKEEQEE"
     gene            complement(8636..9607)
                     /locus_tag="ACG8PO_002148"
     CDS             complement(8636..9607)
                     /locus_tag="ACG8PO_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFQ0207445.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            10313..11692
                     /gene="brnQ"
                     /locus_tag="ACG8PO_002149"
     CDS             10313..11692
                     /gene="brnQ"
                     /locus_tag="ACG8PO_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HFQ0207446.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGSNWI
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYDSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFQPMGNLNQSAADSYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMPLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKSPFVYQVTMFFSAVPAVLDM
                     LANSPALVSQQTVVASLLGFYHHHVPFAALGLGWMVPTLLGYAGSLLFYYAYRLSGYK
                     QEANELPEE"
BASE COUNT         3227 a         2487 c         2146 g         3974 t
ORIGIN      
        1 tctaagatca ttttgaatta atcagtctta tagttgattt ctaaattgtc atatattatt
       61 aaaaattttt gccatttaaa aagaaaagcg acagtttacc aataaatttc aactgaatta
      121 tctaaaagta gatcagttag tttagataat tcagttgaaa ttgaccgact caaattactt
      181 gtataatacc taggaaaaaa ttaactagca ccacgggtat agttatttat atttataaca
      241 aataaaccag agaaatagcc tattttgtgc ctattctctg gtttatttac atattagaaa
      301 ttattttgtg atgatttgat ctaaacttgc tagcgacaaa gccatggcag caatttgact
      361 taattgcgtt aatcggttct tacgtactgc tggttcttct gccatgacca tcgtctgatc
      421 aaaataattt tcgatcagtg ggcgaaggct tacaagctga tcataatttt cgttgatcgt
      481 ttggtcagca aagctttttt tcattgcctc tactgctttg tgcagttctt tctccgcttc
      541 attttcgaac agttcagggt ctacttctga ttgtttttca agctccgctt ttttcgctaa
      601 gttcacgaca cgtgtcaaag cttccatcga tggtttgaag tcttcatctg caagatgctg
      661 cttgaacaat tgtgctgctg caaatacttt tgtcagatct ttttgttctg cattcaaaac
      721 agcttcgatg acatcatgac gaatattctt cgttgtcaat aattggcgca gacggccttt
      781 gataaagtcg atcacctgtt gttgcccttc tgataaacca atgccgaaac gatccgtatc
      841 tttactgata acatcactaa tcgtttcttg cagaacagac aaaggaaagg cccatttttt
      901 cgattcgatg atccgaacga caccgtatgc ttggcgacgt aaggcataag gatcgtttga
      961 cccgcttgga atcatcccaa ctgcaaagaa agaaaaaatg ctatctaatt tatctgcaat
     1021 cgctaaaaca gaacctactg tagactgagg tagctctcct tcactggata taggcatgta
     1081 atgctcacga atcgctgttg ctacagcagg tttctcgcct ttcagcagag catatttttc
     1141 gcccatgatc ccttgtagtt caggaaattc ccctaccatg ttggttacta gatcaaattt
     1201 atagatttca gcagctcgtt gcagatccat caattcttcc tcagatagtc ctacttgttc
     1261 gccaatgata cgggcaatga cttgtacccg ctgcattttc tcatagatac taccgatttt
     1321 ttcatggaaa gtaacagttt ttagtttttc aacgtattgg tcgattgaag ctttcttgtc
     1381 ttcattataa aagaattccg catcttctaa acgagccgtc aatacttttt gattgcctcg
     1441 aaccacattt tccaatttta cccgatcacc attacgagca gaaataaaat gaggaagcag
     1501 cataccttga tcgttccgta cctcgaaata acgttgatgt tctttcatag aagtgactag
     1561 tacttcttca ggaacagaaa gatatttttc ttcgaagtcg ccagaaaaga cagttggata
     1621 ttcgactaaa ttgttgactt cttctagtaa atcttcgtct agatcaataa cccagtgatt
     1681 tttttcggct aattcatttg cttgtgtaac gatcatttct tgtctttttt gcggtgaagc
     1741 aataacgaat tgttcattca atttttcttc atattctttt gcatgcgcaa atgtcacatc
     1801 ttcgcctaag aaacgatgtc ctcgagaggc tctgcctgtt ttgacatcta agatctcaaa
     1861 cgggattact tcgtcatcta acaatgccac gatccagtga atagggcgga tatattcaaa
     1921 atcgtattct gcccaatgca tcgttactgg gaaagtgagg cttttaatga catctgctaa
     1981 acctgtcaat acttctttcg cttctttgcc gtgagtgtat ttcgttacat agacatattc
     2041 cacgcctttt aattctctaa aagtgatagc atctgtcgtc agaccttgtc cgcggacaaa
     2101 tccttcagct gctttcgtcc agtttccatc tgcgtccaaa gcaatttttt tggctggtcc
     2161 tttgacttct tcttcactgt ctgcttgacg ttctggaatg tttgttactt tgattgctaa
     2221 gcggcgcggt gtagaaaacg tttcgatcgt atcataagtc aattggtttt catctaaaaa
     2281 ttttgctgtt ttttcttcta attgtttcat gcttggtgtg acaacatgag caggcatttc
     2341 ttccagcccg atttcaagta ataagtcttt tgccatctta ttcagcctcc ttcatgtttt
     2401 ctggatttgc tgtgttttta ttcaataaag ggaatcctaa tttttcgcgt tctgccacaa
     2461 agattttggc tacagcacga gccatgttgc ggatacggga aagataacct gctcgttctg
     2521 ttacggatac tgcaccgcga gcatctaata aattgaatgt atggctgcat ttcaaaatat
     2581 aatcatacgc tggatgaaca agattttcat cgatacagcg tttagcttct ttttcaaaac
     2641 ggtcaaagtt gcttagtagc aagtcttggt cacttacttc aaaagaatat ttggaatgtt
     2701 catattctgg ttgtttgaag atctcgccat atttcacacc atcagcccat tccagatcat
     2761 agacactttc aacttcttga atatatgaag caagacgttc tagtccataa gtgatctcag
     2821 aagtaactgg tttacaaggt agtcctccga cttgctggaa gtacgtgaat tgtgtgatct
     2881 ccatcccatc aagccatact tcccaaccca caccggcaca ccccatcgat gggttctccc
     2941 agttgtcttc aacaaaacga atatcgtgtt ctaatggatc aattcctaaa agacgcaagc
     3001 tttctaaata aagctcttgg atattttcag gtgaaggttt cattacgact tgaaattgat
     3061 gatgctgata caaacggttt gggttttctc cataacgccc gtcagctggt cggcgggatg
     3121 gctctacata agcagcattc catggttccg gtccgattgc ccgtaagaat gtatatgggc
     3181 tcatcgtacc tgctcctttt tctgtgtcat aagcttgcat cagcatgcag ccgtttgatg
     3241 accaaaattt ttgtaatgtt aaaatcatat cttggacggt caatttttta ctcatctgtt
     3301 ttgccccttt caatttttaa gcgttgctgt caataagaat acaggaaaga aaacgacaaa
     3361 aagtccctat gtctgattac agacataggg acgaataatt tcgcggttcc accctacttc
     3421 cgaataaaga ttcggcagct tcatttatcg tactcgcaag tgcctcttca aaaatcgttt
     3481 gggtccggct tccactctcc cgaactcgct aaaccacgtg ttttttactg atcttggtca
     3541 atgtacgtat tcattttgca gttttcttgt ttatataata gatcctctca gtgaacgatc
     3601 tatttaatct acggttaata atagacggaa gtccctgtat ttgtcaaata gttttgattt
     3661 ttttgttgta tctttcattt attcttcttt ttcaggtatc tctttttcag gcatattttt
     3721 ctctggcgtc tttaatacgt gtgcccaaga cttcatttga tcaatgaact ttttgctttt
     3781 tagatgaata ccgacatatt catcatacaa ctggtcaatg aggtcacgga ttgcctgctt
     3841 cgtttcttct ttcaagtgga ttccactgat cttatcataa gaaatagccg aaaacattcg
     3901 aacgaaataa acagcgcgag gatctgcatg ataacggtgt tcgtccattg gccagtgttt
     3961 ctcgcatatc acaccgccag atttggaaga atagtcaaat tttccttttg tttccccgca
     4021 tacgacacag tgcgtccaaa ttggcgcgat tccgaagcgt tgcagtattt gaacttcaaa
     4081 gatattcgtg atgatttctg catcatctcc ttgatccatc cgctgcagag cttgttgcgt
     4141 aaattggaat aaatgcgggt catattgatg atcttcgatt gctgcatcta ctagatttag
     4201 caaataggta ccataaccgc tcaaaaagat atcttcttgg atgttccgaa acgcatggac
     4261 gtctttgaca ctgcttaaaa acgacaaacc ttcacttcga aaattaccga tatagactgc
     4321 ttcggtaaag gggagcaaag ccggagctaa tggctgattt tgccgatgga ttcctttgat
     4381 ataaaacatc attttgccag aagattccgt aaagattttg actaatttat ctttttcttt
     4441 gtaattacgg ctagataaaa tcaagccttt cgattctgca agctggctca tattctccct
     4501 cctaaaaaat aaatctggaa aagcagattc tgccgatcca gattatttat gtttcgatct
     4561 tccgccgttt ttctcaacgt gcggattttt atccagttta tttctatact taacctataa
     4621 gaagttcaga atatcagtat tcgtcttttc ggtagccaaa gtcttgcaga tagacttttt
     4681 tgtctcgcca gtctttttgg actttgaccc aaagttctag aaatactttg tctcctaaaa
     4741 gattttcgat atcctttctt gctttcgttc ctacttgctt cagcattttt ccgcctttgc
     4801 cgataatgat tcctttttgg ctatctcttt cgacgatgat cgtggcctgg atatggattt
     4861 tatcattctc gtttcgcttc atcgaatcaa caacgactgc gacagaatga ggcacttcgt
     4921 ctcgtgttaa aaacaagact ttctcacgga tcaattcaga aacgataaag tattcaggat
     4981 gatcagtcac ttgatcatct ggaaaatatt gtgggccctc tggcatttca ctgactaata
     5041 catccattaa gcgttcgaca ttgtttcctt ctgtagcaga gatcggaatc acttgtgcaa
     5101 aaggcatctg cgtcgaataa tcttcgatga ttcccagcaa ctcgtctgga tggatcttat
     5161 cgattttatt gataatcaga taaactggag aatgcacgtt ttttagacgt tcaataataa
     5221 aatcgtctcc ccggccgcgt ttttgatcag cactgatcat aaataagact gcatccactt
     5281 ctttcaacgc actgtaagct gcttctacca taaaatctcc tagacgatgc tttggtttat
     5341 ggattcctgg tgtatcgata aaaatcagct gagcttctgg tgtagtatat actccttgga
     5401 ttttatttct agttgtctgt gccttgtcac tcataatggc aattttttga cccacgattc
     5461 ggttcaataa tgtggatttt cctacattcg ggcggccgac gattgcgaca aaccctgatt
     5521 tatgttctgg catatacgtt ctccttttta attaaacaac aaatgatata ttttagggac
     5581 aaataaaatc aacccaatga tgacagcaaa acaagcggtc actaaaactg ctccagctgc
     5641 catatctttg atttttttcc caatcggatg aaaatgaaaa tccgtaaaca tgtccactac
     5701 attttcaaaa acagtattga tgatctctac tatccaaacg agaaaaacag ctaaaagcag
     5761 ccataaccac tctatccgat caagctggaa aatcacgccc ataaataaag caaggatacc
     5821 gaacgcaaca tgtttcctca tattgcgttc ttcctcaaaa acggttttga ctccttggac
     5881 agcaaattcc acagacatca ggaaatgttt gtttttctct acgtttttat cttttgagtc
     5941 cataggcatc caagatctct ttctgtaaac caaacatttc tttctcatct tctggtgtca
     6001 tatggtcata cccgttgata tgcaagaatc catgtaccgc taaaaagcca agttcccgat
     6061 catatgaatg gccgtaagct tctgcttgtt cagcagcacg ttcaatagaa atcatgatgt
     6121 ctcctagtac acgaggcaat ggattctctc catcttcaaa aatcactggg atttcgtctt
     6181 cgccttcttc ttccagagca aaactgatga cgtctgtcgg tgcatctttt ccacggtaat
     6241 cacgattgat tacttggatt gccgcattgt ccatgaaagt aacagacatt tctgtttcct
     6301 ctggcagctt taaataagat gctgcaaatt gcagcaagtc atcgatttct ttcattttct
     6361 cttctgagac ttggtttgtt tcatccataa aggtaatatc cattatacat cgcttctttc
     6421 tattcttctt gttcttcctt tatcttctca acttctgttt tcattttagg atatttgatc
     6481 cgagaatgga aggtgctact taaaccgctg atcaatgatt tttccacttt tcggatttct
     6541 ttcaaggtca gtccactatc gtctaattga ccatcggaaa tacgctcttc gataagatta
     6601 tggacaaaac tttcgatttt ttcgctcgtc ggatgatcca ttgcccgtac tgcagcctca
     6661 caactgtcag caatattgac gatccctgct tcacgacttt gcggttttgg tccaggataa
     6721 cggaattgct cttctgttac ttccggattg cgttctttcg ctttgatgta gaaataacgc
     6781 attagcgtgg ttccatgatg ctgtttgcaa atatcgatca ccatttgcgg catattataa
     6841 tcttccaata ttttggctcc atctgtcaca tgtccaaaga taatatgttt actgtcttct
     6901 ggcaataaga aattgtgcgg gttttccgct cctgaaggca agttctcaac gaaaaagcta
     6961 gcatgtttga tcttgccgat atcatgataa tagcaagcta ctcgagttag caaagaacgt
     7021 ccgccgattt ctgctacagc gttagcactt agattagcta ccatcatact atgatgataa
     7081 gtacctggcg cttcttccag caactgtttc aataaaggat gatttggatt gctgagttca
     7141 tttaatacaa ttacactgtc atcattgacc atcaattcaa tatatgggtg tagaccgatc
     7201 gtcagcaaga atgagaagag tgcaccagtg aacccgcaga caagcattaa ccaagtcgtt
     7261 gcatcaccaa agctcattcc ttgatagacg atcaaaatga catccatcat cacaggaaaa
     7321 ataatgaccc acatggcagc agataatcct tgttctgaga tccgtttgcg tctgacaatc
     7381 gttgccatga gcccagagaa aacataagac atcaagataa tcgttagaaa gttcgtgcca
     7441 atcgaatcat aaaaaatgaa aagtgcagat actgcttgga aaataccagc catgatccct
     7501 gctcttcggt tcaagaaaaa gcttaaaaca agtggtgcaa aagcagcggg ataaaacagc
     7561 gggatataag tagcttgttc cgtttggaat aattggaaaa atttcatcag cactatgctg
     7621 actgacatag ctgtcacata gaaaagcacg tattttgttc tttctcctgc attttggaat
     7681 tgaagtgaat tataaatcag tacgccgatt tgaagcaaaa cagctaaaac catggcaatt
     7741 agcggaaaaa tcgaagttgt ttgattagtc agtcctagta attccaattt tttcatagct
     7801 gaagcatcga tttgattacc ttcacgaaca atgatttctc cttggaagat catcactggt
     7861 tgtacagctt ctcgtgcgga ttgtttcaat tcttctgtct ttttctcatt caatacgtca
     7921 ttcactacga taccttgatc cactaagtag cgaatcattt gttgttgtgc tggtgtgaca
     7981 ttcaaatatt ggatttgctc ttctgcttct tgtttaaaag cttccaattc actttcacga
     8041 acttgtttgc tcatctgttc gtcaatgagc ttcagactct catccctcac agtatggatc
     8101 tcagtttcag tcatttcaaa tatcgtcgaa taaaaattgt tcggtagttt ttgataaaac
     8161 gcaacatttt ctgcattcac attttcaaaa tttttcttca acgcggcgat ccgctcttct
     8221 gtagtaggct ttgggatatt ttcatcttcc tttgccttat caatcttttc gtcataagat
     8281 ttgttgatgg catcattcgt tttatcgatc aattcaaata gttgcttgat ccgattgttc
     8341 tggtcatctg caagatcttt ttggtaagtg tattctggag tgactgcttc tgctgccagt
     8401 tttctttttt gttcggtttc ttccgtgttc tcgatcgttt tgttcgcacg gatactttct
     8461 tctgctactt ggccttctct gaaatcattg gccttttgtt tgacactact aaatgtaacg
     8521 ccgcagataa tgataaaaaa caagaacagc aggaaaggca atgctgcctt tccaagtttt
     8581 tcttgtagtt tctgcagtgt tttattcaac atgtgcttat cctcctaagc aaagcttatt
     8641 cttgatggag atctgccttt tcataagctt tgatgatttc ggcaactaca gggtgccgaa
     8701 caacgtcact tgcttcaaaa ttaacaaaat cgattttctt gatttgcttt aatgttcgtt
     8761 ctgcatgaac aagaccactt gttgtccctc gaggcagatc aatctgactc gtatctccat
     8821 tgacaatcat ttttgattga taaccaagac gtgtcaggaa cattttcatc tgagcaactg
     8881 tcgtgttctg tgcttcatcc aaaataacga atgcatcatc caatgtccgc ccccgcatgt
     8941 aagctaaagg agcaatttcg atcacgcctc gttccatcaa tcggttcgta tgatctaatc
     9001 caaaaatttg gtataaagca tcataaacag gacgcagata aggatctact ttttctttta
     9061 gatccccggg aagaaagcct agattttccc ctgcttccac agcaggccgc gtcaaaatga
     9121 ttttttgcac ttcacctttt ttcaaggcag cgattgctaa tactactgca agaaaagttt
     9181 ttcctgtccc ggctggtcct acgccaaata cgatatcatg tttggctacc gattcgacat
     9241 atttcttttg gccgctgttt tttacccgta ttggttttcc attacggtct ttgacgatct
     9301 cttcctcata catttcaaca aaataatcta atgtgccttt ttgccccatt ttcagtgccg
     9361 tcacgacatc tggtgtactt acatggattc ctcgtttgat caattcctgc aatgtccgaa
     9421 tgactgaggc agccaaggaa atctgggatt gttcgccaat gatctgaatg atttctccac
     9481 gtgtatggat ggttgtttct gttgtttctt caatcagttt tatatgcttg tcgtgtgcac
     9541 ctaaaagcat gctgacatca tcagcagccg tcaatctaat ctctaaagaa ctagactctt
     9601 ccgtcaaaaa tagacatccc ctttttttcg attcattctt tcctatttta ccatactcag
     9661 aagaaactat atagcaaata agaaaaggtt ctaaaagagt tgcttaacca taagtaccaa
     9721 gaaacgttaa aaaatagctg cttccattct ctaaaggtct cgtttgaata aaaaaagctg
     9781 atcatgtaag ctatgcataa gatagaggac aagtattccg tctaacttag caagtattct
     9841 ctgggtattt ttcttgtttc aaaaaaagaa aacaaaatat ttgaaatatt cttgcatttc
     9901 aaaaaaaaca ggtgtattat aacgtcaaca aataaatata cagcaaagcg tcgaggaaaa
     9961 cagtaggcta ttcagcgatt tattcccagt gagtcatgta agtgagaaat gacaataaat
    10021 cgaatagacg aacatcattt ccgagtcaat gtcgaaaacg tagatcattg tggctgcctc
    10081 cattatcagg ctagcggata actttatcga aaagcgtccg tgaatgaaga agctttttta
    10141 agcgaaaaaa ggtggtaccg ttctagaatc tagagccctt ttcccaagtg gaaaagggct
    10201 tttttttata ttttaaggtg gtggttttca tggaatctga tattagtggc atttctagtt
    10261 tctgcaacgt ttattttgtt actcaaaatg ataattattg gaggaaaaat ttatggaaga
    10321 aacaaaaact tattcatgga agcaattatt gatggtcagt tcacttatct tcgggatgtt
    10381 cttcgggtca ggtaatctga tctttcctgt ccacttaggt caaatggctg gatcaaactg
    10441 gatcagcgct ggtgtaggat ttgccatctc aggcgctctt ttcccattat tagctatctt
    10501 agctgtagta gtgacaaaaa gtgatggtct gtatgactta gctaaacctg tcggaaaatg
    10561 gtatgctgcc ttgttcttga tccttgttca tttaacgatc ggaccattct ttggtacacc
    10621 gcggacagca gcaactgctt ttgaaatggc tgctaaaccc tttttgcctg aaaaatatga
    10681 ttcattaggt atgtttttat tctctgctct tttcttttta cttgcttatt tccttactgt
    10741 acatccaaca cgtttggtca aatatgtcgg aaagtattta aactccttgt ttcttgcatt
    10801 attagctatc atttttgtcg tttctttatt ccaacccatg gggaatctaa accaatctgc
    10861 tgcagattct tatcagacga acgctttgac aaatggcgtt ttagaaggat acaacacggt
    10921 tgatgcagta gctttacttg ctttaagtgt cacttttgtc catgcagttc gtgatcttgg
    10981 ttttaaagat aaaaaagtca cagaattaac agcaaaagct ggtagcctga gcgttgcgtt
    11041 agaagtcatg atttattttg gtctggtcct tctcggtgcg atgagtttga ataagatgcc
    11101 tttatcagaa aatggcggaa ctgcactttc tcagatcact aatttttatt taggtaactt
    11161 cggtgctagc ttcttagggg taatggtcac attaggtgtc ttcactaccg ctatgggact
    11221 cgttgtttct tttgcacaag atttccataa actgttccct aaagtcagtt atatgacatg
    11281 gctgcggtta acgacttttg tctcttttgt cgttgcgaat gccggattgg ataatatcat
    11341 ccaatggtct ttaccagtat tgatgctttt gtatccattg tcacttgcgt taatcttgct
    11401 ttccttaaca gcaaaattct tccaaaagag tccatttgtc tatcaagtaa cgatgttctt
    11461 ttctgccgtt ccagccgtat tggatatgtt ggcaaattcg cctgcattag tgagtcagca
    11521 aactgtcgta gcaagcttac taggatttta tcatcatcac gttccttttg ctgcattagg
    11581 acttggctgg atggtcccaa cgttacttgg ttacgctggc agcttactct tttattacgc
    11641 ctatcgccta tcagggtata aacaagaagc aaatgaacta ccagaagaat aaccaaatca
    11701 aacacaattt aacataagag atattaaaca ggaaaaaaac agctagaaga aagaatctta
    11761 ttctttcttc tagctgtttt ttagttcttt ttttacgaac acaaaaaact gcggacaatc
    11821 actgtccgca gttt
//
LOCUS       DAZKKW010000081        11809 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.083, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000081 DAZKKW010000000
VERSION     DAZKKW010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11809
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.083"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            508..1071
                     /locus_tag="ACG8PO_002150"
     CDS             508..1071
                     /locus_tag="ACG8PO_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0207447.1"
                     /translation="MLYIKKGIIVISFFKEYLEIINFRLKTKKESVIILVYFQGGNLM
                     IKKLFLLSILTLAMAGCSDSGLATELITKSTEATTEELKCGNDHSDYRGLNRRCMGIL
                     GRSNCKHRCSNRRRCRRSNRKIKRKIKRSNLCCASYTDVEDAVLLNHVRRCSFMSREV
                     GDEPKLEYAFSFAREDEDNPKTYDILA"
     gene            complement(1132..1497)
                     /locus_tag="ACG8PO_002151"
     CDS             complement(1132..1497)
                     /locus_tag="ACG8PO_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02328 family protein"
                     /protein_id="HFQ0207448.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(1573..1818)
                     /locus_tag="ACG8PO_002152"
     CDS             complement(1573..1818)
                     /locus_tag="ACG8PO_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0207449.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            complement(1888..2142)
                     /locus_tag="ACG8PO_002153"
     CDS             complement(1888..2142)
                     /locus_tag="ACG8PO_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0207450.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            complement(2306..2629)
                     /locus_tag="ACG8PO_002154"
     CDS             complement(2306..2629)
                     /locus_tag="ACG8PO_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0207451.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
     gene            2816..2889
                     /locus_tag="ACG8PO_002155"
     tRNA            2816..2889
                     /locus_tag="ACG8PO_002155"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2850..2852,aa:Ile,seq:cat)
     gene            complement(3274..3771)
                     /locus_tag="ACG8PO_002156"
     CDS             complement(3274..3771)
                     /locus_tag="ACG8PO_002156"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HFQ0207452.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(3811..4620)
                     /locus_tag="ACG8PO_002157"
     CDS             complement(3811..4620)
                     /locus_tag="ACG8PO_002157"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFQ0207453.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            4954..5811
                     /locus_tag="ACG8PO_002158"
     CDS             4954..5811
                     /locus_tag="ACG8PO_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFQ0207454.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            5804..6706
                     /locus_tag="ACG8PO_002159"
     CDS             5804..6706
                     /locus_tag="ACG8PO_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFQ0207455.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            6739..7089
                     /locus_tag="ACG8PO_002160"
     CDS             6739..7089
                     /locus_tag="ACG8PO_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFQ0207456.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            7138..8061
                     /locus_tag="ACG8PO_002161"
     CDS             7138..8061
                     /locus_tag="ACG8PO_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFQ0207457.1"
                     /translation="MDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPDDIFVG
                     TEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLITCIDR
                     KTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAARKTTEN
                     EELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQRQTEK
                     MIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTGGLPGK
                     SSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            8139..9032
                     /locus_tag="ACG8PO_002162"
     CDS             8139..9032
                     /locus_tag="ACG8PO_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFQ0207458.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKDAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            9070..9633
                     /gene="amaP"
                     /locus_tag="ACG8PO_002163"
     CDS             9070..9633
                     /gene="amaP"
                     /locus_tag="ACG8PO_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HFQ0207459.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            9647..9835
                     /locus_tag="ACG8PO_002164"
     CDS             9647..9835
                     /locus_tag="ACG8PO_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HFQ0207460.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            9848..10750
                     /locus_tag="ACG8PO_002165"
     CDS             9848..10750
                     /locus_tag="ACG8PO_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFQ0207461.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            10782..11024
                     /locus_tag="ACG8PO_002166"
     CDS             10782..11024
                     /locus_tag="ACG8PO_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFQ0207462.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(11280..11651)
                     /locus_tag="ACG8PO_002167"
     CDS             complement(11280..11651)
                     /locus_tag="ACG8PO_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0207463.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
BASE COUNT         4191 a         1988 c         2235 g         3395 t
ORIGIN      
        1 tcattggtcg ttgaatgggc tgaatagtag tgtataaaac aattttgtgg agcgtgaagc
       61 ttttatggta gaggaaaata cgaataaagt gatgttgata aaaagattcc tatcttgaat
      121 atgaatcgag atgtcttgaa aatgaatgag tttgaccaat cattatccta tcgaaattta
      181 cagtttcgtt cgattggtct gtcaattaat gaaaaagtaa aaaagaatat gttctaggaa
      241 gagggcggca catctaaatt tttaagtaac ggacagttca aatatcaagc attgaaagag
      301 gctatcctac tgtttgaagg aatacgaata tataaaaacg acaaagagga aaaagtagtt
      361 cattaaatcc cttcaagtga tgaaagaatg agatctaata aaacctgatc tttcatctgt
      421 ttttttaata acgtaataat ttttttattt tttgtgcttt cgccgttatt gtagagtgat
      481 ttagcattct tatttttaaa aataactgtg ttatatatta aaaaaggcat aatagttata
      541 agctttttta aagaatattt agaaattatt aattttcgtc tgaaaacaaa aaaagaaagc
      601 gttattattt tagtctattt tcaggggggg aaccttatga taaaaaaact atttttacta
      661 tcaatattaa cgctagcaat ggcaggttgt tctgactctg gcttagcaac tgaactgatc
      721 acaaaatcaa ctgaagccac gacagaggag ttaaaatgtg gaaacgatca cagtgactac
      781 agaggcttaa atcgacgatg catgggaata ttaggcagaa gcaattgtaa gcatcgatgt
      841 agtaatcgaa gaaggtgtag aagaagcaac agaaaaatca aaagaaagat caaaagaagc
      901 aatctgtgct gtgcatcgta tacagacgtc gaagatgcgg tacttttaaa ccacgtcagg
      961 agatgttcat ttatgtctag ggaagttggc gatgaaccaa aactagagta cgcttttagt
     1021 tttgcacgtg aggatgaaga taaccctaaa acttacgaca ttttagcttg atctaagatg
     1081 gttaaagctt tttaatgaag taagaggaga tagaaggctt ttctagttat attaggtaag
     1141 ataaattcct ttttgctcca aatttgctaa acattctgct agataaactt catcgtgttc
     1201 cggataaatc ggcacgttgc gctctacagt aggtaaatca aaaaatgctt cgcatgattt
     1261 gccacgatac ataggatttt tccactctgg tgctgggctg taaccacgtt tttccatttc
     1321 atccatgaca agctgatgat attggaataa tctataagga gaatagtgaa aaacataatc
     1381 cacagtagca tgtttctttc cccagccatt tcctcttaat gcacagcatt cccgatgttg
     1441 tcctaaaagc tgttgacgag gaagctttga aatcaatttt tcatgccata atcgcataga
     1501 aatgtctcct ttatatatag attgttaacc ttagacgtta aatgaaaaaa ccagcaccaa
     1561 ggctggaaaa aatcacttca gtttgcttgc tgtttctgag cgaccaatta ggtaatcaac
     1621 ggaaatatcg taaaagtcag cgatatagat taattcaatg atgcttggaa tttcttctcc
     1681 ttcttcgtac ttacgatatt tgaataaagg aatacttaac gcgctagaaa cagctacttg
     1741 agataaagat cggccagttc ttaagaaatt cagacgacaa cgtaaagtat ccagtaaaat
     1801 agcttcttcg atactcattt aatcaccttt cattatagta gaatagttat aatgactata
     1861 gaaagctaca catgcaatca atagtactca atgtttttta tttaaacgta tcaaataaag
     1921 aatgacgatc aataaagaca aaaccagtcc tgataattct tctataaaaa cactatcggg
     1981 ttcaaagtag ttgtaattaa aaactttcca cataaagtag atagccggaa gagtcaaaat
     2041 agctaaataa aaccgttctt tgttccaatt cataagataa acaatacttg cgaccagtgc
     2101 gaccgcaggc acccaaaaca aaactctcac caacgtaatc atgtaacgga ctcctcaacg
     2161 attcatataa taaaagataa catgttattg catacatcac aaatgaaatg ctaggaaaat
     2221 aaaagaagaa tagatcaatg aaaaatgaaa aagcagaaca gttttctgct gataagcagg
     2281 aactgttccg ctgaatgtgt cttatctatc gagaaaaaat caatggattg atatctaagt
     2341 aattcaatac atataaaaag aggacgatcg cattcactaa aaaggaaaga atgatgatcc
     2401 catagcgaat ggaaacatat ttaacaaata cttttgtttg aatacgggca tatctttttc
     2461 ctttatagct gagcatcatg acaagaagca aaagggtaat cacaccttga aaaatcattc
     2521 caataaacag aattcctgag gtttgataat ttaattggag aaaataagct gctgccgcaa
     2581 ctaaatcgaa taaaatcgca ataaatggca aaataatcac tcctaacatt gagaataaat
     2641 aaaagaataa cattttttct aaaataagca aataaaaatt aaagtaacaa aatagttatg
     2701 acacaagtaa aagaaataag aataacaata ttattttact tatattttga aacttgtgag
     2761 agtttgtcat tcgtgctata gttgaaaagt aacaaaaata aattttattt atgatgtacc
     2821 tttagctcag ttggttagag cagacggctc ataaccgtcc ggtcgtaggt tcgagtccta
     2881 caaggtacat aagaggttgt gaaaaagctg ccctgcatca agtaataaga acagccaagt
     2941 aaaaacagct tcttatgttt ttacttggct gttcttatta ctgcagatgc aagcttttga
     3001 ataccgttta aggaagaggt tgtgaaatca gcgttttgac ctgagtatta gcttaacaaa
     3061 cggaaaaata gttcctcata ttttgccgtt tgttaagcta ataccgaggg tcagctgatt
     3121 gaacaccatt taagaaagag gtcgtgagcc tgccgtttca ctctgtgtca taaaaaagcc
     3181 aatctacttt gatagaaaaa gcacgtagga ctaaaacagt tctgcgtgct ttttttattg
     3241 tgtgttaaat atcgtaaatg aagatagata tttttatgat tcgacgatag agacgttgaa
     3301 atctttcaac agctctaaag cggattctcc gtgttttcta ctggagacta acattggatc
     3361 aacaaaaaag tctgcttcag gaaaagcact ctgcaggcct atcagattag agagtacaca
     3421 caagtttgtt tcaatcccga ctacttcgat ctctttttgt tcttctttgc tttcaaataa
     3481 agtttcctgg atttcagcta aagtttcagg tggaatcgta aagtaatact ttttaatcac
     3541 tcgttcattt tcttgtggag aaagaagagg gatcaattgc aaatcttctc gttcttcttc
     3601 tttgtccttc agttcaactg gtatatcccg tgtgaagaga atatattcat tattttggcg
     3661 tgctttagct aatctttgag caatacgttc tgccaattga tctgactctg gaatataaaa
     3721 ttcgctgttt ggatcaagaa tatgattttg catatcaata ataactaaca ttttctttcc
     3781 tcctacattc atatcgtcat tttattcttt ttaatagaag ttttttaaaa tataagcaac
     3841 accagcttct tcattcgtca gtgttacttg gtttgcaatt tcttttactt gtcgctgtgc
     3901 attccccatc gcaatactca acccgctgta attcaacata gcaatgtcgt tttcttgatc
     3961 gccaaaagcc atgatgtcac tcgattcgat tccaaggtaa tccgccagaa aaacaagtga
     4021 atgagcttta gtgatacctg taggcgtgat ttctaagaaa tgcggatcag agaatactga
     4081 ttggatacca tctgcttgca gatctctttg tagcttctgt agtttttctt cactcttatc
     4141 tgttaatgct aatttaagat agctaagaga aggatcttcc aacatattta aaatttgatc
     4201 aaaagaatgg ttctgtaaat tgaaatgttc ataataaggg tcatctttga ctagatttga
     4261 ttcatcgaat gtcagctgat catcttgggt ttctcggcta gcaatgatgg atattttcgc
     4321 ttcttgagca tagaagaagg cttttctcag tgtatcgcta tctatttttg aacgtctgag
     4381 aactttcgcc atttttccat ccttcatttc agcaatcagt gaaccattgg aagaaatcaa
     4441 atagccaccg attcctaatt cggaaaaaat atctttagca gaaatgctgt tccttccggt
     4501 agcgacggtt acgattccgc cattttcatc aaatttccga agtgcttgga tattttcttc
     4561 tggtatcttt ccatttcgat caagcaaggt accgtctaaa tcaatcgctg ctagtttcat
     4621 tttatcttcc tactttcttt ttacatatct attttcctgt taccttaaga ttaaatgaga
     4681 atttaaaaaa atgaaactaa cttgctttgt cattatgaat tttttcaaaa aacattctta
     4741 agaaaaaggt ggctatatat agtagttttt ggtcaatacc tttgaataaa aagccatgaa
     4801 aaacgaatag ttagcgctta ccgatgcgtt ttgtgataaa aaaattttaa aatattcaat
     4861 gaaaacaaag catatatttt gctttaaaac caaatgagga atattctaga tgtagaacag
     4921 gttagaaaaa gattagaaaa aggagtgatt ttgatggaga agagatacgg cgtagttcct
     4981 gtggtaactg cttcagacga aaattatgct ccttacttga gcgtgatgat cgcaactgca
     5041 ttagaaaatt gcaataaaac aagacgcata aaattttatg tgattgacga tggtctatca
     5101 gaatatagta aagaaggatt ggaagaaacg gtaaataaat attcaagtaa tgcaagtatc
     5161 caattcctga cagtggaaaa agatatctac gaagacttcc tagtcagtga ccatatcaca
     5221 actaccgctt acttaagaat ttctttacca aacttattag ctaaagagga ttataagaaa
     5281 gtcctttact tagattcaga tgtgttagta ctagacgata tcgtaaaact atatgatgag
     5341 ccgttaaatg gaaaaacaat cggcgctatt atcgatccag gccaagtgaa agctttggaa
     5401 agattaggaa tcgattccga tgacttgtac ttcaattcag gagtgatggt gattgacatt
     5461 gatcaatgga ataaaaaaga aattacagaa aaaactatcc attacctcag tgaaaatggc
     5521 gatcgaatca tctatcacga ccaagatgct ttgaatgctg tactttatga agattgggaa
     5581 caacttcatc caaaatggaa catgcagact tcgctgattt ttgaacgtca tccagcacca
     5641 aatgaaaaat atgaacgtca gtataaagaa ggaaatgaaa aaccttcgat cgttcacttt
     5701 acaggacatg ataaaccttg gaatacgttg aaagatcatc cgtataccaa tttatactta
     5761 aaaaaattag cacacagtac tctaacgaaa gtaggcgaga tcaatgaata aaaaagaaat
     5821 agcagtagtc gcaagttgta acacaaaatt cgtaccacat ttagctgcat tatttgtctc
     5881 agtattagat aattgcaacc cgtctaaatt tgttcggttc tatgtgattg acgatgatat
     5941 cgattttgaa agtaaacagc ttttacgctt ctcagtgaaa aacgcacgaa tgaactcaga
     6001 tgtggaattc ttgaaaatca ataaagaatt ctttacaaat gtggtaatca gcgatcgtat
     6061 tccagaaaca gcttattatc gtattgcgat tccggaattg ttccgcggca cagaagttga
     6121 acgtatctta tatatggatt gcgacatgat cgctttacaa gatatctcta aattatggcg
     6181 tcttgacttc ggtgattcaa tcgtagcagc tgttgaagac gctgggttcc atcaacgact
     6241 ggaaaaaatg aaaatttcag ctaaatcaat gcgttatttc aactcaggtt tgatgctgat
     6301 caatgtgaaa aaatggttag atgaaaatat tacgcaaaaa gtattagatt tcatcgaaca
     6361 taatccagaa aaattacgat tccatgacca agatgcatta aatgctatct tacatgaccg
     6421 ttggttacca cttcatccgc gttggaatgc acaaggctat atcatggcaa aagcaaaaaa
     6481 acatccaaca gcagcaggtg aacgtgaata cgaagaaact cgcaacaatc cttacattat
     6541 ccatttttca ggacatgtga aaccgtggag caaggatttt gaagggccaa cgaagaaata
     6601 ttacgaaaaa tatgcaggga tgacggcttt ccgttgtgta gctaaattcc ctaaatatcc
     6661 aaaatatgca aaagttcaac caaaaacaga tgtagttgtt aaataataga agacaacaac
     6721 tgaagagggg gagtaggaat ggaaattaca atgtctaaaa atgcagtaga gacgttaatt
     6781 gaaaaggttg gggaaaatac aaaaatcgca ctcgcgctta tcaatgattc cgatccattt
     6841 ctaagggaca aaggtgcttt tgcaaaaggc agcttttttc aaatcattcc ttttgtttcg
     6901 gaatttggag aatatgcgac taaaatcgag catccattat tggacatcta tacatctaaa
     6961 ttagagcaaa attatttcgg taaacgattg aacatggact tcaacaaaca gcttgacagc
     7021 ttttcattag aaaatgaaat cgcagtactc gattataata tcaaattaaa gaactgcttc
     7081 ttcagctaat agtttcgaga aagtccaaaa aggaggtata cattgagata ctactttgtg
     7141 gatgaagaaa agaatgtaat agaaacagat gaaaaaaaat ataattggct cgtacttgat
     7201 agtgcaaagc ccgaagagat tgagcaagca atcgaagaat tcgagcttcc tgatgatatt
     7261 ttcgtaggaa ccgaatatcc ggaagaagtt tctcggatgg agcgccttta tgagacgaaa
     7321 ctagagcatc cattttcttt ggtagtgatt aatctagatc agtcagataa tcgtatcgaa
     7381 aaaaaattaa cgccaatatc ttttgtactt tccgataatc tcttgattac ttgtatcgat
     7441 agaaaaaccg aatttgtgga tcggctgctg acaaagcatg gaaatcattt aaactcgttt
     7501 gaaaagatca tcacgtatac attattagat atttacaccc attacgtgaa agaactaaga
     7561 gagatgaaaa agcgaattga cgctttggat aaagcagctc gtaaaacaac ggaaaatgaa
     7621 gaactgtata aacaggcaga tcttgaaaga gacatcgtat atatcgatca taccttaaga
     7681 gatcaacggg aaacaatgga tcaattatgg aattctgaaa ctttcatcaa acgtttagac
     7741 gatgaacagc tactctatga tgtccatttg agacagcgac aaacagaaaa aatgatcgag
     7801 atctatcgag atttattaga gagtattggt gatctattca acgggatgat ggacaataat
     7861 ctaaatcata tcatgaaata tctagattca gctgcattaa tcatttccgt tccggcgttg
     7921 ttcgcaggga tttggggaat gaatacagga ggattaccgg gtaaaagttc tgctctaggc
     7981 tttatactcg tagtctttgg ctctttgata gcagcagtag ttttaggtta ccatttatac
     8041 cggaaagatt atacaaatta aagaatctat ttaaaacagg tatcaaacaa agaaatttgt
     8101 tttggcaata caatttaaaa actaggagga atacagcaat gacagaacca aaattaatcg
     8161 acccgcgaaa cttgtaccat acggagaaat ttcctaaaca agatcaggat acaccagctt
     8221 tacagcagaa tatgattcct gttcctgatt gtggagaaga atcctatgaa ggcaataatc
     8281 aactagaaaa ccgccgtgta ctaatcactg gtggagattc agggatcggt cgtgcagcag
     8341 cgattgcatt tgctagagaa ggggcagata tcgctcttca attcttccca ggagaagaaa
     8401 aagatgcaga ggaagtagca aaatacgtga aagatgcagg acgtaagatt gtcttactac
     8461 cttttgatct tcgtgacgaa caagcaccta aagaaatcat agataaagcc gtgagtgaac
     8521 ttggcgggct agatacgatg gtactaaacg caggacagca aatttcttgt cagtctattg
     8581 ctgagttacc acttgagcaa gtcaaagaca catttatgat aaacatcatc gctatgtttg
     8641 ctttagtgaa agaagctgtt ccgcatatcc cagcaggagg agcaatcgta acaacaactt
     8701 ctgttcaagc attcaatcca agcgaacatt tattagatta cgcagcaaca aaagcttcaa
     8761 tcgctaactt tacgatcggt ttagcaaaac agcttgcacc taaaggaatc cgtgtaaatg
     8821 gtgtagcacc tggtccaatc tggacaccat tacaactaga cgaaggacaa ccttctgatt
     8881 cgatccctga atttggtcag caaagtttac tagaacgtgc aggtcagcca ggagaattag
     8941 caccagttta tgttttctta gcatcgaata aagcaagtta tgttacagcc caaatttacg
     9001 gagtaactgg tggagaagcg atcaacttat aagaagttga tcgtttcacc cctatgagga
     9061 ggtgcgaaaa tgaataaagg tgtaaaaacg atcggagtga tcggaacatt acttttgcta
     9121 tctgtgcttt tgtttgcgat actattaagt gcaacctatc cattgccttt tcagctagag
     9181 aagtttcgat tcttttccat cactaattca tacgtgcaac aatatgtatt ttggcttgcc
     9241 gttgcatttg ctattttgac aattatcacg ttacttgttt tcctgtttta tccaaaaaca
     9301 atcggaaact ttctactgaa acatggtgat ggggagttga cgttggataa aaaagcaatt
     9361 gaaggaatgg ttcgttctca cttgcatgaa gatgaattca tccattcacc aaaagtaaat
     9421 gtcaaagcaa cgaaaaacaa gatcgccgtc aatgtaaaag gggagttgaa acgaacttca
     9481 gcactcattg gtaaaacagg aacattgatg aaagaaatcg aaaatgaagt acaacacata
     9541 ttgggaacaa aagagccagt gaaagtatcg gtgaaataca caggctttga agaaccagct
     9601 aaacgaagca atgaccgcaa acgagtagaa taggggggga ccaaaaatga aagaactatt
     9661 ttcgacatac aagctcccta tcatctttgg ggtacttgga ttgatattag ccgttttatg
     9721 gatcaccgtg ggctttttca agaccttatt gatcttgatc atggcagcga tcggcgtcgc
     9781 tatcggtttt tatctgaaac aaacgaaaat cttagatcac tattttaaat cttaggagga
     9841 aacatccatg aataacaata acaaaaataa taacaataaa atgaacaacc aaaaaccagt
     9901 gtctgcacat ccagacccaa cgacaccaaa ccaatcaaat ggtgtgaacg atgaaaagaa
     9961 caaaacagca ccagtatctg cacatccaga tccagtagtc cctaacgttc aacaaggagt
    10021 agaagagaag aaacatgatg cagaaaagca tgcacctcat catccaggaa ctccagaatc
    10081 taaagttcca gaacaacatg atgcaatcga tccttctaaa cctgtggaag agcaaaaaca
    10141 tccttctgac catgcgaaag cacaagaaaa acttagttct gaccacacaa acggacaagg
    10201 aaaacttggt tctgatcata caaataacca aggaaagcat gattctgaac aggaagattc
    10261 aatgcgcggt gagctgaatt atgaagataa agttgtgcaa aaaatcatcg gaatcgcaat
    10321 tgaacaagta gacggtttat tatctgcaaa cggtggtttc ttctctaatg tggcaggaaa
    10381 acttgtgaat acagataatg taacggctgg gatcgaaaca gaagtcggta aaaaacaagt
    10441 agcagttgat atggatgtca ttgtggaata tggcaaagat atcgaaaaaa tctttgaaga
    10501 aatgcaagaa gtaatcggca aagaagtaaa aaatatgaca catttagaag tgatcgaagt
    10561 caacgctaac gtagtggaca tcaaaacaaa agaagaattt gaaaaagatc aagaaacggt
    10621 gcaagacaaa gtaactgacg cagcgaaaaa aaccggtgaa tttgcttcga atcaaacaga
    10681 taaagtaaaa tcagcagctg gaagcggcgc acaaaaagta aaagaaaata cagaaccacg
    10741 ggttcagtaa taaggaagta aaacattagg aggaacgaaa catgggattt atttggtcac
    10801 ttatcgtcgg aggaattatc ggagcaattg caggagcaat cacgaataaa ggttcttcga
    10861 tggggattat tgcaaatgtc gtagcaggtt tagttggatc agcacttggt caagcattat
    10921 taggttcttg gggaccaagt attgcaggaa tggcaatcgt gccatcattg attggtgcca
    10981 tcattttagt actaatcgta tcattctttg ctcgaaaact gtaaaaagac ttttaaaaag
    11041 aggtcgtgaa aaagctgttc ttcatcaagt aataagaaca gccaaacaaa aatagcttct
    11101 catatttttc atttggttgg gcatattatc gaagatgcaa gcttttgaac actgtttata
    11161 cggaataaga ggctgtgaca agacttttgt cacagcctct tatttatgcg ggcaaattcc
    11221 caattagatt tttaaaagtt tttccttaaa tgtgtcttta ttgatacaga gaagcttctt
    11281 taataaaaca atgcctccct aaattttctt gagataggaa aggtgaacat acaaaataat
    11341 acgaccactt tgaaaaccca gatgatccac aaatggatcg tatgatactg atgaagtaaa
    11401 aagcaatgga tcagaaactc aaagagattg aaaaagacaa ggaagaaata atatctcttt
    11461 tttactcttg tctgttttag ccaataatag ctcgtaaagt aatctagaca aacaaataga
    11521 agtaaacaaa gtttcgtaag agcagcaatc gtatcatgtt gctctgctag acgattgacg
    11581 agcaggaaaa atgttggtaa tggaatatag atcacgagta atgaaagaaa aaagtaaaca
    11641 ataaatggca tactattcct cctaattgtg tgagttcgcc ctcattttaa aataacgata
    11701 tttgtttgtg aagctttttc gtgtcgtaac aaatgttatc taatgtattt ttaaataaat
    11761 aatcattatt atcaaaataa tgttaaaaat aaaaaagaat tgtccatca
//
LOCUS       DAZKKW010000082        11243 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.084, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000082 DAZKKW010000000
VERSION     DAZKKW010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11243
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.084"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(396..569)
                     /locus_tag="ACG8PO_002168"
     CDS             complement(396..569)
                     /locus_tag="ACG8PO_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0207464.1"
                     /translation="MSMLVRKLPLSVREFLFYLVCLIASGFKLIEHLFIFAYILAMIA
                     FLSGGWLIIYLLS"
     gene            complement(572..970)
                     /locus_tag="ACG8PO_002169"
     CDS             complement(572..970)
                     /locus_tag="ACG8PO_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0207465.1"
                     /translation="MQLRRNKNYTTIILEDFTDQELLIVGKTKADFIKKGNASIVKAS
                     KVAVEKYFDSLEESAKEIFNGEKAGSYELDGIRFSLIPTTENIIPKELYYIFSSEKHT
                     SMKPCIFDWQWLIDAVLDVKCSKYNITLGC"
     gene            1186..2241
                     /locus_tag="ACG8PO_002170"
     CDS             1186..2241
                     /locus_tag="ACG8PO_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0207466.1"
                     /translation="MKEPLLFSVDLGNKSVKEMSTHVSPLSLPSRILNKNIINSGRFS
                     NIGSSLKRSLNVSEYSLVNTTESYYFGKDIHELGKDDYVIESLGFGISRYKQETYQNI
                     LSFSLANLARLEDQSDDVLTVNLVLGLPSSDYTETIVKYVMQQATRQHSVEVDGKAYN
                     INVDTVWVLPQPIGTLYNLLLADDGSVQDETLISQKIGFVDIGGGTKLFDVVQNFEID
                     ENNREQKDTGIFTLYNRIVNMLDLPISPNAFQLEKIIREGKEKGVYLFKPSGNESFDI
                     SEVVEREIKTYTNMVVAEVLATFKNFTTLDKVVFTGGGASIIDKQLVKDKIPHTLFVE
                     DSEFSNVKGFYKFGLTQ"
     gene            2261..2614
                     /locus_tag="ACG8PO_002171"
     CDS             2261..2614
                     /locus_tag="ACG8PO_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0207467.1"
                     /translation="MNKGKSERYVVIINREKVSELPLAEFLESEYETLGVKYSQTIKK
                     ALNLYRRVKNNEVTIIEAGNDRNDSKVAEVKNKPTDHNPIQQEHPSTVTSDHSAKKEK
                     PMLKGFNAPSNKDLL"
     gene            complement(2796..3083)
                     /locus_tag="ACG8PO_002172"
     CDS             complement(2796..3083)
                     /locus_tag="ACG8PO_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0207468.1"
                     /translation="MKENRFKKFAEAEVEEVPEVVEAVEEPKQTPKETTSSLVAKLKS
                     DSSEKKKNYQFTLQPSQRKKLSKISEKLGIRSDSALLGEMIDNLYDEVFGE"
     gene            complement(3096..3914)
                     /locus_tag="ACG8PO_002173"
     CDS             complement(3096..3914)
                     /locus_tag="ACG8PO_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0207469.1"
                     /translation="MEKKLSIITGNIEKGGTGKTTTIYNLGGYAVENLGMKVLYIDED
                     KSQNLSKRFPDAFSKAKRENTINHLYLHGSAEPLAINENMDILVAGEDLKQVEMQMRD
                     MPNNRTLLFRWIVVNYEQLKEKYDLILIDTHNDAGLLAQNAWAVSDLVVGISDPSKDG
                     FEALLKLGKDIDALAKQLIDIKTGETLMETKYATIGSRIKHNTNSSREFLELVPTLSN
                     YLGYVQEKELLNATSLEGKNIAEFAKDKNIYRKNKDFFESTFTLYNKIIDTAQE"
     gene            4152..4373
                     /gene="relB"
                     /locus_tag="ACG8PO_002174"
     CDS             4152..4373
                     /gene="relB"
                     /locus_tag="ACG8PO_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB family
                     antitoxin"
                     /protein_id="HFQ0207470.1"
                     /translation="MPVISIRVSKEEKDFLEKVAEFNGDNISEFMRSNSLQTAETLVD
                     FATYKELMKEHEENDQSISHEDMLKELGL"
     gene            4375..4641
                     /locus_tag="ACG8PO_002175"
     CDS             4375..4641
                     /locus_tag="ACG8PO_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFQ0207471.1"
                     /translation="MSYQVRYEREAQKTLKKMDKFQAKVILNWIEKNLVGTDDPRKHG
                     KGLTANKSGYWRYRVGAYRIIADISEHEVTILILSIGHRSEVYK"
     gene            4808..5293
                     /locus_tag="ACG8PO_002176"
     CDS             4808..5293
                     /locus_tag="ACG8PO_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0207472.1"
                     /translation="MGWYGAYSSANEVIEEKKGYAKNGLEIVDTKVTKTYGIILFKDA
                     EGTYDIDSFIFRDEMYKPIYFLNDSPAYFKKIPKKWLKFFVGDQEKMVKRQLDRIKEE
                     SNKPKLDDLLVAGKTYSIWDGSYVAVYQYKLKRSFIFRLQDGSLTRFTRLSAGDVKEV
                     A"
     gene            5784..6068
                     /locus_tag="ACG8PO_002177"
     CDS             5784..6068
                     /locus_tag="ACG8PO_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0207473.1"
                     /translation="MLKATLQQPTHVAIAESYTINAGVVKQLIKDREFKKFNSRQAYN
                     RQLKKAILKHKNSFIGKLESVDVKTVSTAFSVVEVTIDGRLISLFAFGIN"
     gene            6075..6320
                     /locus_tag="ACG8PO_002178"
     CDS             6075..6320
                     /locus_tag="ACG8PO_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0207474.1"
                     /translation="MWTLEGKTFAEIWTEFIEVPTRIDPITSEKVLDADFYNLKAGTS
                     LECVWMYLEEHFKGVTLGHVLYHNGAEWDAIGNIHFS"
     gene            6342..6596
                     /locus_tag="ACG8PO_002179"
     CDS             6342..6596
                     /locus_tag="ACG8PO_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0207475.1"
                     /translation="MNYTDEEQITLEYVFTNEEGEEIEKVLIISSKDLEKALAYEPYE
                     STVTVIEFLDTYTSNDTWEIMNICNANNIQFAEKTNKSFY"
     gene            6678..6863
                     /locus_tag="ACG8PO_002180"
     CDS             6678..6863
                     /locus_tag="ACG8PO_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HFQ0207476.1"
                     /translation="MPKTAREIVKILKSNGFKKTGQTGSHAKFYNSKTKRTVVVPIHN
                     GDVPLGTEANIWKQAGL"
     gene            6930..7325
                     /locus_tag="ACG8PO_002181"
     CDS             6930..7325
                     /locus_tag="ACG8PO_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HFQ0207477.1"
                     /translation="MKTKNIFYPAIFEKEGEAYNIVFPDLPEISTFGNSLEEAYLNAK
                     DALGLALYSVPDAEFPKPSAVEELTLKANQVVVIIQLNIKLFRRSLNTKTIRKNVSVP
                     EWLVLLGKEKNINFSQLLQKALEEELLEK"
     gene            7342..7692
                     /locus_tag="ACG8PO_002182"
     CDS             7342..7692
                     /locus_tag="ACG8PO_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0207478.1"
                     /translation="MKATHLNYFSRTLINNLLKDTTGISVSTKHFANDLIIFEDNNLR
                     IEMFIEQATPNSIPEFCYTHTEKMKHAKSVKDVEALSESLTNALNLVKKAKLLFEFQT
                     DYHNELLAQSEILD"
     gene            7714..8088
                     /locus_tag="ACG8PO_002183"
     CDS             7714..8088
                     /locus_tag="ACG8PO_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0207479.1"
                     /translation="MKQTQTETVNDTLADQLAGYYKLFEMSKAQRLIHYLWKYKILFL
                     FWEATLTFSVLFYKSDFYNQHLVLGSFLLIYTILMLNLYTFITLEENLVISDAGIERN
                     IKRVSIKITLNKPTDKKLSEAF"
     gene            complement(8396..8794)
                     /locus_tag="ACG8PO_002184"
     CDS             complement(8396..8794)
                     /locus_tag="ACG8PO_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HFQ0207480.1"
                     /translation="MKYFFCAVFEWKGNEYAVKFPDFPDLYTHGETLEDAFEMAQEVL
                     EGYLVLLELQEKEIPSPTPVQELNARIKRSENVNLFVVKANTKVAKARNKQNYDRKSV
                     TVEHYLNVLAAEENINLSKALNDLLREKLI"
     gene            complement(8986..9753)
                     /locus_tag="ACG8PO_002185"
     CDS             complement(8986..9753)
                     /locus_tag="ACG8PO_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFQ0207481.1"
                     /translation="MKKDELTKYNEEFLATIDMKMDLKSYELYQLLFSYISPTKLKKT
                     GVVDLPVKAIINYMDVDQNLETELKTIVNTLQRQAVYEISNLDKKYKTYTTVSAIDRI
                     HWDLEEHSVSFSFSEDVIPLIVSIKKKYPFFVFEDLASFQKKYSIVLYKYLAMYYKKY
                     LIAKNKGDFEKAEIYKNPLIEIVKIREITKTENEYKTISNFDRRVLRESVEEINEKSM
                     LKIDFAKVRKGRNYNFAVQFNISSEFDEKPYREKKLG"
     gene            complement(9789..10949)
                     /locus_tag="ACG8PO_002186"
     CDS             complement(9789..10949)
                     /locus_tag="ACG8PO_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFQ0207482.1"
                     /translation="MKITEKNVNPKFEPTESNQKMFDLTVTLSNQLVDSIVEMDAMPQ
                     KIYLYAIGHLDPKNPPFNNTVKVSKKALFALMSSSSSSSKHSRLKQVIEKLHQQSVIK
                     IQRTKTNGKTEYKVISPLEITEFNDFSDAVSIKFSSSIMPLLTNLEDHFTQYTFDQLV
                     NLTSKYAISFFQLFTKDFNLYEANRDNLTWSTKSLDRLKNPVFSIAELRKKFSIEKKY
                     KAFRDFERRVIKEPIAQLNKYTDFKIDYELIKKGHTYTDIKFLLTRTMSVPVPYKQEQ
                     RDPAYLKGAEDKTEKMNQSYLKAMNSKYTKLLIKNFLISSADLCDLKFMSRLQNKVYP
                     LYDRLSKDRGFESVVNHLSYVATHKEDYTIKNTVKYLEKSITSYLPYVNLYD"
BASE COUNT         3858 a         1919 c         1747 g         3719 t
ORIGIN      
        1 tttattagca ggaaaatcta aatattttta caagaaatcg tagaaatata ttggtttcgc
       61 cttcagatta atactggatc atattaatct gaatacaata tatgaactaa taaatagctt
      121 aacctcgcta ttttgttttt tctgatcatt atctacacag cattaacaaa tagaatttta
      181 aaaataaaaa aagtaagaca aaaaaagtat caacataaac atatacatat acacgtttat
      241 gttgatacac ttaatttgtc ctttaaaaag cactgatttt taaattgcgt tattttataa
      301 cccgaataca gtctgcttgt taggcattgt ccttaaatta catttttcat aggttattta
      361 atcatttaat ctgttgatta ttcttgatct aaaacttagc ttaacaaata tataatcagc
      421 caaccgcctg ataaaaatgc gatcattgct aatatataag caaagataaa aagatgttcg
      481 atcagcttaa accctgatgc gattaagcag accaagtaaa atagaaattc tcttactgat
      541 aatggtaatt ttctaactag catactcata attaacaccc caaagtaata ttgtattttg
      601 aacattttac atccagtaca gcatcaatca accattgcca atcaaaaata caaggcttca
      661 ttgaagtatg tttttcagat gaaaagatat aatataattc ctttggaata atattttcag
      721 ttgttgggat caagctaaat ctaattccat ctaactcata gctaccagct ttctctccat
      781 taaaaatttc cttagcactt tcttctaaag aatcaaaata tttttcgaca gcaacctttg
      841 aagcttttac tattgaagca tttccctttt tgataaaatc tgcttttgtt ttaccgacaa
      901 taagtaattc ttgatcagta aaatcttcta aaataatggt agtgtaattc ttatttcttc
      961 tcaattgcat caggatcact ccttaatata atttatgttt ttataatatc ataataagat
     1021 aaatttacaa gtatttgttt acaccttttt atttacactt tattataatt aattatactt
     1081 ttatgattgc atttttttct tataaacagt tgcaactaat agtaaatagg tgtaaactat
     1141 tttcgtaccg agataccata aacacaaaca gaaagaggtc ataccatgaa agaaccatta
     1201 ttgttttccg ttgacttagg gaataaatca gtaaaagaaa tgagtaccca cgtttctcca
     1261 ctctcacttc cctctcgtat tttgaacaaa aatatcatta attcaggacg tttttctaat
     1321 ataggttcct cattaaaacg atcactaaac gtttctgaat atagcctagt aaacactact
     1381 gaaagctatt actttgggaa ggatatacac gagctaggaa aagatgacta cgtgatagaa
     1441 tcactaggtt ttgggattag cagatacaag caggaaacct accaaaatat cctatctttc
     1501 tctttagcta acctagcacg tttagaagat caatctgatg acgttttaac cgttaatcta
     1561 gttttaggtc tgccttcatc cgactatacc gaaacaatcg tcaaatacgt gatgcaacaa
     1621 gccacacgac aacattcagt tgaagttgac ggtaaggctt ataatataaa cgtagatacc
     1681 gtttgggtac tgccacagcc aatcggaacg ctatataacc tcctacttgc tgatgatggt
     1741 agcgtacaag atgaaacctt gatcagtcag aagatcggtt ttgttgatat tggtggtggc
     1801 acgaaacttt ttgatgttgt ccaaaatttt gaaattgacg aaaacaatcg tgaacaaaag
     1861 gataccggca tttttacttt atataatcgt atcgtaaata tgcttgacct tccgatcagt
     1921 cctaatgctt tccaacttga aaagattatc cgagaaggca aagaaaaagg cgtatatcta
     1981 tttaagccaa gcggaaatga aagttttgat atttcagaag tagttgaaag agaaataaaa
     2041 acatacacaa atatggttgt tgcagaagtt ttagctacat tcaaaaactt cacgacctta
     2101 gataaagttg tctttactgg tggtggagct tctattatcg acaaacaact tgtaaaagat
     2161 aaaattccgc ataccttgtt tgttgaagac agtgaatttt ctaatgtaaa aggcttttac
     2221 aagtttggtt taactcaata aaacagcgaa ggagatcaac atgaataagg gaaaatcaga
     2281 gcgttatgta gttataatca atagagaaaa ggttagcgag ttacctttag cagagtttct
     2341 tgaatccgaa tatgaaacac taggcgttaa atactctcag acgatcaaaa aagctttgaa
     2401 cctatatcgg cgtgtaaaga acaatgaagt tacaattatt gaagcaggca acgatagaaa
     2461 cgatagcaaa gtagcagaag taaagaataa accaactgat cataacccta ttcaacaaga
     2521 gcatccttca acggttacat ccgatcattc agcaaagaaa gaaaagccaa tgctaaaagg
     2581 gttcaatgca ccaagtaaca aagacttact ttaaaatgaa aaaggcgagg ttacaatacc
     2641 tagccttttt attatgtaca aatataaaca taaacatata cgtatacatg tatataaaca
     2701 tgtatacgta tatatccata aacacgtata tgaaatttag ttaagctata cctaaccgct
     2761 tattccaata aaaaaagtga tcagctttaa actgatcact caccaaaaac ttcatcatac
     2821 aagttatcta tcatttcacc aagtaaagct gaatctgatc gaattcctaa cttttcgctg
     2881 attttagata gcttctttct ttgcgatggt tgaagcgtga attgataatt tttcttcttt
     2941 tcagaactat cactttttag tttagctacc aaagaacttg ttgtttcttt aggtgtttgt
     3001 ttaggttctt caactgcttc aaccacttca ggaacttctt ctacttcagc ttcagcgaat
     3061 tttttaaatc tattttcttt cacttcaatc actccttatt cttgtgccgt atcaattatc
     3121 ttgttgtata gcgtaaacgt gctttcaaag aaatccttat tcttacgata gatattctta
     3181 tccttagcga actcagcaat attcttacct tctaagctag ttgcattaag caattccttc
     3241 tcttgtacat atccaagata attagataac gttggtacca attctaggaa ctcacgactt
     3301 gaattagtat tatgtttgat cctgctaccg atcgtagcgt atttagtttc catcagtgtt
     3361 tcaccagtct taatatcaat caactgtttt gctagtgcat caatatcttt accaagcttc
     3421 agcaaagctt caaaaccatc tttagatgga tcagaaatgc ctacgacaag atcagataca
     3481 gcccaagcat tttgagctag tagacctgca tcattatgag tatcaatcag aattagatca
     3541 tacttctctt taagctgttc atagttaaca acgatccatc taaacagtag ggttctgttg
     3601 ttaggcatat cacgcatttg catctcaact tgttttaaat cttcacctgc tactaaaata
     3661 tccatgttct catttatagc taaaggttca gctgatccat gaagatataa atgattgatt
     3721 gtattctctc ttttcgcttt actgaaagca tcaggaaagc gttttgatag gttttgcgac
     3781 ttatcttcat caatatacag caccttcata cctaaatttt cgacagcata tccacctaga
     3841 ttgtagattg ttgttgtttt acctgttcca cctttttcaa tgtttccagt aatgattgac
     3901 aacttctttt ccatgattaa acctccctta ttaacctttt gtaactccat tatacactat
     3961 aaacaccttt atgtatatgt ttatatacat aaaggtgttt atatttatat ttgcttttat
     4021 attctataac ataaacatat atatgtatat gtttatgtat atgtttatgt ttatgtatat
     4081 gttcatatat ataactattg catctgtcat acaatcgtag tacaatgtaa ttgaaaagga
     4141 gttgataaac tatgccagta ataagtattc gtgtatctaa agaagaaaaa gattttcttg
     4201 aaaaagttgc tgaattcaac ggagataaca tttctgaatt tatgcgtagt aattccctgc
     4261 aaacagcaga aacgttagta gactttgcca cctacaagga actaatgaaa gagcatgagg
     4321 aaaacgatca aagtatttcc cacgaagata tgctaaaaga gttaggctta taagatgagt
     4381 taccaagtaa gatatgaacg tgaagctcaa aaaacgctaa aaaaaatgga taagtttcag
     4441 gcaaaggtta tcctaaactg gatcgagaaa aatcttgtag gcactgatga tccaagaaag
     4501 catggtaaag gtctaacagc taataaatca ggctactggc gatatagggt aggtgcatac
     4561 cgtataatag ctgatattag cgagcatgaa gtgacaattc ttatattgtc tattggtcac
     4621 agaagtgaag tttataagta acagacaagc tgataaggct tgtctgtttt tttgttaacc
     4681 caacaaaatc actgtttacg atcccacttt acctaaaaaa gaacaatatc ctcttgttat
     4741 tatacacacc agtgtgtata atataagtgt aaacaagcta attaactttc aaaaggagcg
     4801 attttttatg ggatggtatg gtgcttattc atcagcaaat gaagtaatcg aagaaaagaa
     4861 agggtacgcc aaaaatggtt tggaaatcgt agataccaaa gtaacgaaga cctatggaat
     4921 tatccttttc aaagatgcag aaggaaccta cgatattgac agcttcatct ttcgagatga
     4981 aatgtacaaa ccaatctact tcttaaatga cagtcctgct tactttaaga aaattccaaa
     5041 aaaatggttg aaattctttg tcggtgatca agaaaaaatg gttaagcgac aactagatag
     5101 aattaaagaa gaaagcaata aaccaaaact tgatgactta ttagttgcag gtaaaactta
     5161 ctcaatctgg gatggatcgt atgtcgctgt ttatcagtat aaactcaaac gtagctttat
     5221 ttttcgacta caagatggat cactcactcg gttcactcga ctatcagcag gagatgttaa
     5281 ggaagtagcc taaaacttaa atacataaac ataaacgtgt atacgtttat gtttatgtat
     5341 atgtatatga atataaacgt attaggtacg aacgctttta aatgcttttc tgggctttga
     5401 ttttagggta gggctaaaac atcaccatct gatccaaaac agctcagaaa cgcatttaaa
     5461 cgtatctgat accaatatta cagaaggagt gtatcgtatg attaacctat ttcttttagt
     5521 gacagctagt acattattct ttactggaat atcacttata atttcctatt tgatctataa
     5581 gaaagccgaa acttacacaa gcaaagcaac cggactagcc gtttcatcct ttttaacttt
     5641 gctgactttt tttgttactc tatcgttttc ttttgtttag cacagctgat ccatcagcaa
     5701 aaaacctctc tttacattat acacactagt gtgtataatg taattgtaaa caagctaatt
     5761 aacttttagg aaggaatgac aatatgctaa aagctacatt acagcaacct actcacgttg
     5821 ctattgcaga aagctataca attaatgcag gggtcgttaa acagctgatc aaggatcgag
     5881 aattcaaaaa gtttaacagt cgtcaagcgt ataacagaca gttaaagaaa gctattctaa
     5941 agcacaaaaa ttccttcatc gggaaattgg aaagtgtgga tgttaaaact gtttcaacgg
     6001 cattttcagt tgttgaagtg actattgacg gtcgcttgat ttctctattt gcttttggaa
     6061 ttaattgata gggagtgtgg actttggaag gtaagacttt tgcagaaatt tggactgaat
     6121 ttattgaggt accaactcgc attgatccga taacttctga aaaggtattg gatgcagact
     6181 tctacaatct caaagcaggt actagccttg aatgcgtttg gatgtattta gaagagcatt
     6241 tcaaaggcgt aacgctcggt cacgtcttat accacaatgg tgccgagtgg gatgcaattg
     6301 gaaatatcca tttttcttaa tacaagaaag gagaacacaa tatgaattat accgatgaag
     6361 aacaaatcac tttggaatac gttttcacaa atgaagaagg ggaggaaatt gaaaaagtct
     6421 tgatcatttc atcaaaagac ttggagaagg ctttggctta tgaaccatac gaatcaaccg
     6481 taactgtcat tgaattctta gatacctata cttctaatga tacttgggaa attatgaata
     6541 tttgcaacgc taataacatt caatttgctg aaaaaaccaa taaatcattt tattagtttt
     6601 gcaataaaac atcctctttt tctattgatt ccatacacaa caatgtgtat aatagatttt
     6661 gtaaagggag ggtagctatg cctaagacag caagagaaat tgtaaaaatt ctcaaatcta
     6721 acggcttcaa aaagactgga caaactggca gtcatgcaaa attttataat tccaagacta
     6781 aaagaacggt cgttgttccg atccacaatg gtgatgtacc actaggaaca gaagctaata
     6841 tctggaagca agcagggctt taacagcctt gccttcctat ttggtattag gcatactatc
     6901 ctctcttaca ctagaaaagg agcgaggaaa tgaagacaaa aaatattttt tatccggcaa
     6961 tcttcgagaa agaaggagaa gcttataaca tcgttttccc tgacttaccg gaaatatcta
     7021 cctttggaaa tagtcttgaa gaagcatatt taaacgctaa ggatgcttta ggattagcgc
     7081 tatatagtgt accagatgca gaatttccta aaccatcagc agtggaagaa ttaactttga
     7141 aagccaacca agtggtagta atcatccaac taaacattaa acttttccga agaagcttaa
     7201 atacaaaaac aattagaaaa aatgttagtg ttccggaatg gttagttctt ttagggaagg
     7261 agaaaaacat aaacttctct caactacttc aaaaagcact agaagaagag ttgcttgaaa
     7321 agtaaaaagg agaccgaacc tatgaaagct acacacttaa actatttctc tcgaacgtta
     7381 atcaataatc tattaaaaga tacaactggc attagcgtta gtaccaaaca ctttgccaat
     7441 gatctaatta tttttgagga taataacttg cgaattgaga tgtttattga acaggcaacc
     7501 cctaactcaa tacctgaatt ctgctatacg cacactgaaa aaatgaaaca tgcaaaatca
     7561 gttaaagatg ttgaagcatt aagtgaatca ctaacgaacg ctttaaacct cgttaagaag
     7621 gcaaaattgt tatttgaatt tcaaaccgac taccacaatg agttgcttgc tcaatctgaa
     7681 attttagatt aattgaaaca ggagaagaga aagatgaaac aaacacaaac agaaaccgtt
     7741 aacgatacgc ttgctgatca attagcaggt tattataaat tatttgagat gagtaaagca
     7801 cagcgcttga tccactatct ttggaaatac aaaattttgt ttcttttttg ggaagcaaca
     7861 ctaacattct cagttctttt ttataagtca gatttttata atcaacactt agttttaggg
     7921 agttttttgc ttatctacac cattttgatg cttaatttat ataccttcat cacacttgaa
     7981 gaaaacttag taattagcga tgcagggatc gagagaaaca tcaaaagggt atctatcaag
     8041 atcacgttaa acaagcctac cgataaaaag ctctcagaag cgttctgaga gcttttgaca
     8101 tttgcttgta taaaatttat cctaacacat tacaaagcac ctaattcgag cttatatcaa
     8161 gcaaatttgc tatttattac tcaatacata tctaaaaatt acttgatgga aaactagata
     8221 catggtaagc atgaaaagaa aatcaatttg agcagatgca gtataataca tagcaattta
     8281 aaagctctca gaagcgttct gagagctttt aatatattct ttgataattt gtctttctta
     8341 aatccatacg tcctaaaaca agctgttatt gcttaatatg atcatttgag agacatcaaa
     8401 tcaacttttc tctcaacaaa tcattcaaag ctttcgacag attaatattc tcttcagccg
     8461 ctaaaacatt taaatagtgt tccacagtca ctgattttcg atcgtaattt tgcttgtttc
     8521 gagctttagc aactttcgta ttagctttta cgacaaataa attaacattc tcagatcgct
     8581 tgattcttgc atttaactct tgaacaggcg taggcgatgg aatttctttt tcttgcaact
     8641 ctaaaagcac caagtatcct tccaatactt cttgcgccat ctcaaaagca tcttctaaag
     8701 tttcaccatg cgtatataag tctggaaaat ctgggaattt aactgcatat tcatttcctt
     8761 tccattcgaa tactgcacaa aaaaagtatt tcatcgcatt gcacctctct aattaaaaaa
     8821 taatttttgc tttttagttt caaaaaaaag aatcgcctac caaaaaaatt gataggcgat
     8881 cacaagctaa ttaacctcta aaggagatta ataccttgct cccaaggcac tacatatctt
     8941 agcatacgtc tgaagcatct tcaaacgagt aagatatatt ttgcattaac ctaatttttt
     9001 ttctctataa ggtttttcat caaactcact agaaatatta aactggacag caaaattgta
     9061 atttctgcct tttctaactt tagcaaaatc aatcttcagc attgattttt cattaatttc
     9121 ctcaactgat tcccttaaaa cacgtctatc aaaattcgat attgttttat actcattttc
     9181 tgttttcgta atctcacgaa ttttaactat ttcaatcagg ggattcttat atatttcagc
     9241 tttctcaaaa tctcctttat ttttggcgat aagatatttt ttataataca tcgccaaata
     9301 tttgtataac acgatgctat attttttttg aaaagatgct aagtcttcaa aaacaaagaa
     9361 cggatacttc tttttgattg agacaattaa gggaattaca tcttctgaaa aactgaaaga
     9421 tacactatgc tcttctaaat cccaatgaat ccgatcaata gcagatacag ttgtatacgt
     9481 cttatatttc ttgtctagat tcgatatttc ataaactgct tgtctttgca aagtgttaac
     9541 tatggttttt agttcagttt caagattttg atcaacatcc atgtaattaa taatcgcttt
     9601 aacaggtaaa tctactacgc ctgttttctt tagctttgtg ggcgagatat aactgaacag
     9661 caattgataa agttcatacg actttaaatc cattttcatg tcaattgttg ctaagaactc
     9721 ctcgttgtac tttgtcagtt catctttttt caaaagcttt tccttccttc acgcaagttt
     9781 ttcagttgtt aatcataaag gttcacataa ggtaaataac tcgtaattga tttttcgaga
     9841 tatttaacag tattttttat tgtgtaatcc tccttatgag tagcaacgta agatagatga
     9901 ttgacaactg attcaaatcc tctatctttg gataaacgat cataaagcgg ataaacttta
     9961 ttctgaagcc gactcataaa tttcagatcg cataagtcgg cagaactgat caaaaagttt
    10021 ttgattagta atttagtgta tttgctattc atagctttca agtacgattg attcattttc
    10081 tctgttttat cttctgcgcc ttttaaataa gcagggtctc tttgttcttg cttataaggt
    10141 accggtactg acatggtacg tgtaagcaag aactttatat ctgtataagt atgacccttc
    10201 ttgataagct catagtcaat tttaaaatca gtgtatttat tcagttgggc aatcggttct
    10261 ttaattacac gtctttcaaa gtctcgaaaa gctttatatt ttttctcaat actgaatttc
    10321 tttcgtaact ctgcaattga aaaaacgggg ttttttaatc tatcaagtga ttttgtactc
    10381 catgttaagt tatctctatt tgcttcatac agattaaaat ctttagtgaa taattgaaaa
    10441 aaactgatcg catatttgct tgtcaagtta actaattgat caaatgtata ttgtgtaaaa
    10501 tgatcctcta agttcgttaa caaaggcata attgaactac taaatttaat agatactgca
    10561 tcagaaaaat cattgaattc agtaatttct agcggtgaga tgactttgta ttcagtctta
    10621 ccgttagttt tagttctttg aattttaatg acagattgct gatgcaattt ttcaataact
    10681 tgttttaatc ttgaatgttt tgaagaagat gaactagaag acatcaacgc aaataaagcc
    10741 tttttagata ctttgacggt attattaaaa ggcggatttt tagggtcaag atgtcctata
    10801 gcgtaaagat aaattttttg tggcatagca tccatttcaa caatgctatc tacaagttga
    10861 ttcgataaag taacagttaa atcaaacatt ttttgattgc tttcggtagg ttcaaatttc
    10921 gggtttacat tcttttctgt tattttcata cgattgcctc ttctttctaa gcttggtacg
    10981 agcttaaaaa cttggaactg gtacgattcc attgaataag tatagacctg ttttcgatac
    11041 gatacaataa gaaatgtatc gtaaatttat aacataactc aatctcagta aaacgcattt
    11101 tatttttatt aatcaaattt cattgataag acagcagaaa tcgctatttt agaaccttta
    11161 caaaataaaa atccttttca aaaaaaatac gtaattataa aaattcacaa aattaaaaaa
    11221 caaatatttt tttagttttt aga
//
LOCUS       DAZKKW010000083        11146 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.085, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000083 DAZKKW010000000
VERSION     DAZKKW010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11146
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.085"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            6..305
                     /locus_tag="ACG8PO_002187"
     CDS             6..305
                     /locus_tag="ACG8PO_002187"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0207483.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="ACG8PO_002188"
     CDS             378..1739
                     /locus_tag="ACG8PO_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HFQ0207484.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFLSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLLFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGILTMPIYLYGATYLAPILSDSAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="ACG8PO_002189"
     CDS             1761..2021
                     /locus_tag="ACG8PO_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0207485.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="ACG8PO_002190"
     CDS             2035..2787
                     /locus_tag="ACG8PO_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HFQ0207486.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="ACG8PO_002191"
     CDS             complement(2881..3486)
                     /locus_tag="ACG8PO_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFQ0207487.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIEQAREWTDHLLTK"
     gene            complement(3542..4516)
                     /locus_tag="ACG8PO_002192"
     CDS             complement(3542..4516)
                     /locus_tag="ACG8PO_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HFQ0207488.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DCPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4894..5613)
                     /locus_tag="ACG8PO_002193"
     CDS             complement(4894..5613)
                     /locus_tag="ACG8PO_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0207489.1"
                     /translation="MPKYEEIANILRERIRSKEYPPDSFLPNQVDLVKEFGASRMTIK
                     KAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEYEGLSLQMKRRHRSLKSQIIH
                     FDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVLEHTFMPVHLVPGLKKEHLLA
                     SIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAENDPVLEVQQVVYLKDGQPIE
                     YSSSRNRFDVRNYSVLDVRST"
     gene            complement(5740..7011)
                     /gene="celB"
                     /locus_tag="ACG8PO_002194"
     CDS             complement(5740..7011)
                     /gene="celB"
                     /locus_tag="ACG8PO_002194"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0019191 - cellobiose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFQ0207490.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7164..8561
                     /locus_tag="ACG8PO_002195"
     CDS             7164..8561
                     /locus_tag="ACG8PO_002195"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFQ0207491.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWNHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSRLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKAIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGTIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8642..9082
                     /locus_tag="ACG8PO_002196"
     CDS             8642..9082
                     /locus_tag="ACG8PO_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFQ0207492.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9229..9555
                     /locus_tag="ACG8PO_002197"
     CDS             9229..9555
                     /locus_tag="ACG8PO_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFQ0207493.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9556..10305
                     /locus_tag="ACG8PO_002198"
     CDS             9556..10305
                     /locus_tag="ACG8PO_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease prefix domain 1-containing protein"
                     /protein_id="HFQ0207494.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10331..10741)
                     /locus_tag="ACG8PO_002199"
     CDS             complement(10331..10741)
                     /locus_tag="ACG8PO_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFQ0207495.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNK"
BASE COUNT         3536 a         1948 c         2291 g         3371 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttcgaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcctgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtctta ttgctgcttt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattacca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcctc acgatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgattcagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaaggaccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcaaaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgggagat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc tcgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccaatc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa aaatgaagta gcatgaagag
     3541 attattcacg ttttttattt gcatcacttg tatcgaaagg acggacatag gcttcaatgg
     3601 cttcacgacg ttcttctaag aatggaggaa gagatagttt ttctcctgct gattctgctg
     3661 gttcatcttc aaggaatcca ggacaatcag ttgctaattc aaacagtacg tgatgagcgg
     3721 ctaggaagta ctcagattgg aaatagaaac gatcgacgaa accagaattt ggcaattcaa
     3781 tcttattgat tacttgaatc cagtattcca atgcttcttt gtctgctaca cgcaatgcta
     3841 agtgatggat atttccaaat ccttcgattg cttttggcaa gtctgtccga tgttcaataa
     3901 tgatgcttgc accatttccg ccttcaccga tttcaaataa gtagaaatct cctgaatggt
     3961 ctgtttcttt gaatcctaag attttttcaa gaatcaagcg gatatgctct aattgactga
     4021 cacggacaaa gacaggtcca agtcctgtga tcgcaaactc tgtcggtaca ttgctgtttt
     4081 tccaaggaat gccagctgct acaccttgat tgttttcgtc agagatcaat tgataatact
     4141 gattatcgaa atcttcaaat tcgatatatt ttttgccgaa acgttcattg attgctgaat
     4201 gagtaacgtc atatttttca aaacgttcca accaaaagtc taatgctgcg tcacttggca
     4261 cacggaaaga agcacgagag atcgtatctg ttcctttttt tccttttggc tgatttggaa
     4321 aatcaaagaa agtcatatct gttcctggcg caccagtatc gtctgtaaag taaagatggt
     4381 aagtttcgat gtcatcttgg ttaactgttt ttttgattaa gcgcattcct aaaatatctg
     4441 taaaaaagcg gtagattttt gtagcgctag atgtcattgc tgtgacgtgg tgaattcctc
     4501 tgatttgtgt atccatgata aatttcctcc tgtcaactta caaaaagtaa gtagttgatt
     4561 tagacatagt atagagggtg catctctatt ttgcaattaa tttgtcttct aaagtatcag
     4621 gaaggaatca agaatggaaa agaggaggga cggatcaata aaataaaaag acttacgact
     4681 agctgtatcg atttcaaaaa aactagcttt aaaaagctaa ctttagaatc attacgggtg
     4741 gtcataagtc tttttatgct gagttatttt ttcatttttg agctaatgct taggatagga
     4801 tagaatgttg atttcacgat ctcttcttgt tacttgaaac tgcttactac tgtcacgatc
     4861 tcttggtaaa cggctttttc acaacctctt catttaagtt gaacgcacat ccagcacaga
     4921 atagttccga acatcaaatc ggtttcggct ggaagaatat tcaatcggct gtccatcttt
     4981 gagatagacg acttgctgaa cttccagtac aggatcgttt tctgcacagt tcaaatattg
     5041 ctgatcatat tcatcacttt tatctgcaga aatagcacga taagcccctg caaaatggac
     5101 attcaattct tgatgaagat gatcatagat agaggctaac aaatgttctt tttttaaacc
     5161 cggcacaaga tggactggca taaaagtatg ctctaaaaca tatggctcgc catctaagat
     5221 ccttaaccgg tgaatattgt agacaggttg ctcggcatcg atcatcaatc gttcttggat
     5281 atattgatta gggaattcaa catcaaaatg aatgatttga ctcttcaaag aacgatgtct
     5341 gcgtttcatt tggagactca aaccttcata ctcgttagcg ggcgatgtgt ctttttgcat
     5401 aaatggatga ttcaagattt tcgttcctga tcctcgctgc gaatagacaa gtccttccat
     5461 tgctaagata ttgatggctt ttttgattgt catccggctc gcaccaaatt ctttgaccaa
     5521 gtccacttga ttaggcaaaa aactatcggg aggatactct tttgaacgga tacgttctct
     5581 taaaatattt gcgatttctt cgtattttgg cacgttattc acctcttcta gagtataacg
     5641 aaagattatg cttccgacaa ctcgaagtag catctggaac attgtttatt cgaaaaaaga
     5701 ggtcgtgaca agtttttgtc acaacccctt aatgatctat taagcaatat ctaaatctgg
     5761 ttgtttatct agtactttca ggaatgggta ccagataaag gcaacgatga caaagtcgat
     5821 gacttgcagc ataccaccaa gccatgaatt tgttgctaag acaccgcttg caatgattgg
     5881 cacagtccat ggaacagaga cgccagttgg tgcagggaca agtccagcag ccattaccaa
     5941 ataattgaac gtcgttacga ttagaggtgc cacaacccaa ggaataagga ttgttgcatt
     6001 caatacgatc ggtagaccga agatcgctgg ttcatttaca ttgaaaatac caggtgctaa
     6061 agctaatcgt ccaacatcac gatactgttt ctttttaaga acaaaggcca tcaataaaac
     6121 gacagctaaa gtcattccag agccaccaat accaacagtg aaggtttcca tgaatggttt
     6181 tgtgatgatg tgtggtaatt cttttccatg ttgataagct tctaagttat caagcatcag
     6241 tgtattccaa atcgggtcca ttacagagtt gacgatgatt tgtccgtgaa gtccgaagaa
     6301 ccataagaat tggacaaaga acaaagcaat caatgttgca ggtaaactac ttcccaaacc
     6361 agttaatggt ttttggatca cttcataaac gacgtcatgt aagttagcat tgaaaatacc
     6421 agtcatcaca gcattgatta ctaagaaaat agttagtgtc actacagcag ggatcaaagc
     6481 ggcaaatgat tttgttactg caggcggaac tccatcaggc attttgatct gccagccgcg
     6541 tttcgtgatt ctgcagtaga tttctccagc caagaaagca gcgatcattc caatgaacat
     6601 tccttttgcg ccaagacgat caagtgaaag cacaccagtc acatcttcac cgccagcggt
     6661 tttcattgca aaaggcgtaa gaatcaagaa gctggcaaaa gacacggcac caccgaaaat
     6721 accttcaacg tcataagatt ttgataaata atagccgata ccaaaagtaa caaaaaccga
     6781 catgatgctc attgttgcat tttgcccatt accgaataag ttcgataaag ttccctttgt
     6841 tgcgtcactg aaaaaaggca agttattgat aacgacaacg atcgaaccga acatcgttaa
     6901 agggaaggaa agcatgaaag catcccgcag cactgtaagg taacgatttt gccctaataa
     6961 attagctaag ggcatgattt tctcagaaag cttgtccata aaaccgttca tttttattca
     7021 cctcaaattt atttgcaatc gattacatac aaagtataac gccaaaaatt ttaaatgtct
     7081 agtcattttc ataaaaagta ttgatttatt ttttagggtt cgttatgatt acgatatcaa
     7141 gaaaaaagag aggaagtaaa aaaatgaaaa cctacacatt cccagaaaat ttttggtggg
     7201 gttctgctgc tagcggaccg caaacagaag gaagagtaag tgatgatggt aaaggagaaa
     7261 atatttggaa tcattggtat gaattagagc ctgaaaaatt cttcgatcaa gtaggacctg
     7321 aaaaaacttc gcaagtttac acaaaataca aagaagatgt tcagttaatg aaagaaactg
     7381 gccataacac atttcgtaca tctatccaat ggagtcgttt gattccagaa gggacaggtg
     7441 aaatcaatca aaaagctgtt tctttttatc gagattattt tactgaattg ctggataatg
     7501 gtatcgaacc ttttgtgaac ctttaccatt tcgatatgcc actaccactg caagaaaaag
     7561 gcgggtggtt atcaagagaa acggtcgacg cttatgtttc ttatgcagca gcgtgctttg
     7621 aactgtttgg agatctggtc aaaaaatggt ttactcataa tgaaccaatc gtacctgtag
     7681 aaggaggcta cttgtatcaa ttccactatc caaatcatat caatatgaaa gaagcggtcc
     7741 aagtaggcta tcatgagaat ctagcaagcg caaaggcaat caaagcctac catgaaggcg
     7801 gacatgatgg tgagatcgga atcatcttga atctgacacc aagctatccg agagatgaaa
     7861 cgaacccaga agatgtaaaa gcggcgaaga tcgctgacgc attcttcaac cgatcattcc
     7921 ttgaccctgc agtgaaagga gagtttccac aagagttgat cgaaattgtc aaagaattgg
     7981 ctataatgcc agagatccat gaaggagacc tagaactcat ccgtgaaaac acgattgacc
     8041 tgctaggaat caattactat cagcctcgtc gaatcaaagc aaaagaaact ccaatcaatc
     8101 acgaacatag ccccatgcca gatgattatt ttgataatta cgacatgccc aataaaaaaa
     8161 tgaatccata tcgtggatgg gaaatttacg aaaaaggaat ctatgatatt ttaacgaata
     8221 caagagaaaa ctatggtaat atcaaatgct atatttctga aaatgggatg ggggtcgaag
     8281 gagaagaacg atttgttaac agcgaaggaa ctattgaaga tgattaccgg attgagtttg
     8341 ttacggatca tttgaaatac gtccatcaag caatccaaga aggaaccaat tgtgtgggtt
     8401 accacatgtg gacatgtatg gacaactggt cttggacaaa tgcttataag aatcgctatg
     8461 gctttatcgc tgttgatttg gcacaagacg gtaaacggac gatcaaaaaa tcaggtcgct
     8521 ggtttaaaac ggtctctgaa aaaaacggat ttgatgcctg atattttttg ataaaacgtt
     8581 cacaaaatgt ttgttctaac actgtaatcg tgctacacta acagtgaagg aggcgtttac
     8641 aatggtagat acttatcgta atattttagt agcaattgat ggttctgaaa aggcagaaaa
     8701 agcatttctt gaggcaatca cgattgccaa acgaaatcag gcaacgttgc acatcctgta
     8761 tgtcaatgaa gtaacaggca attactttgg tgattttgca tttgttacaa caagccttca
     8821 ggaagaattg gatgaggttg cggaaaacca gatgaaagaa catcgaaact tggcaattga
     8881 aaaaggcttg acagatatcg agacatatgt tctttacggt tatccaaaga cactgattgc
     8941 taatttccat gaatcgaaag aaccaatcga tctgattgtc atgggggcga caggtctgaa
     9001 tgcggtagaa cgtgcactag taggttccac tacttcctat gtagtcaacc acgcatcatg
     9061 taacgtcatg gttgtaaaat aaataaatta agggaacaga aagcaaaaaa gcataacgct
     9121 tcttttgctt tctgttcctt taattttttg taaaaaatta ctttacctga tgaagtaatc
     9181 atgctattct aattacaacg acagatggag taatatggag gaaagaaaat gatttcaagt
     9241 gatggaatac gcggatacaa tgatgcgatt attttatcga tccttaacaa aaacgattct
     9301 tatggatatg aaatttctaa aacgattcgt gagaggacaa atgattccta tgtgatcaaa
     9361 gagacaacac tttactccgt ttttgcccgt ttagaaaaga atcaactgat tgtttcgtat
     9421 cctggagaag tgactcatgg aaaaaagcgg acatattata aaatcactga aatagggcgt
     9481 aatcttttag aagaaaaaaa gcaagagtgg ctgacgacga aagacgtagt cgatcaattt
     9541 ttggagggaa attaaatgga tacaatccgt aattatttag acagtctttt tatcggagta
     9601 cctcaaagta ccgagataga taagctgaaa actgatttgt tggcaaatat ggaagaccat
     9661 tatcatgaat tgatgggaga aggaaaaaat gaacaggagg ccatcggtac agtaatttcg
     9721 acatttggtt caatcgatga attattagaa gaactagatg tcgaaaaaaa acatcaagct
     9781 gacgagacgg aaacaaacac agcttctatt tatttaagcg aagcagaaaa ctactggaaa
     9841 gaatacagag cggcaagtct ccaagtggca tcaggtgttc tattcattag cttatctttt
     9901 gcaagttttc ttttcttttg ttcagcaggg tatgtgttca tgggtatttc ttgtctgatt
     9961 ttcggtatag cattagctgt cggttttttt attgcttctg gtatgaaaat cacacgttta
    10021 aatcactttt tgcaccatcg gaaaatacct gaaaaagtat tagcagaagc aaaagaaaaa
    10081 gaagaagagt atcagcgaag ttttggcttc tcactgattg caggaattgg attatgtatt
    10141 ttttcacttt tcccactttt ggcttcttta atgtggtata tggatggtag tatcggtgcc
    10201 tctatatttt ttgttacggt aggaacggga gtcttcttga tcatttatgg cagtctggtc
    10261 cgtcattctt atcgtcaatt cactcaaagt gcttattact ggtgaatgta ttcagatttg
    10321 taaaaatatt ttatttattg cttacccatg taaaatcgtt atagacagat ttcagttgac
    10381 ttctgcgcca ttgtacaggg ataaaaagac gcatactatc ttcattgaat ccgactaata
    10441 atttctttcc gtcagtaatg atgggacgtt gtaacaaagt aaaatgcgag acgatcagat
    10501 cgacaagctc tgaggtcgta cattggtcga tttgatcttt caaattttta taatcaatcg
    10561 agcgtttagc taatagatcg tcaaatccgt tttctgttaa tgacaataaa taaaatatct
    10621 ctttccttgt taaaggctgt ttaggtgtga aaattcgttc tctgtaaggc atttgatggt
    10681 ctttcagcca gtttgccatc agatggtaac tatgtgtgcg agaagaatag atcgtaagca
    10741 taaaaaaact ccttaaatag tccattaatt tgttagatct gttgagataa ataataggta
    10801 aggagctgtg cataagcatt gatcaaaaca acccaaacat cattagaaat aactttctaa
    10861 agtcttaaac ttgcgacgag aaacaactag actaatagaa aaaatgtctt agccatttgg
    10921 cttcctgttt gtttttttgc ttatgctgca gctcctcggg aaagggttct aacaatttga
    10981 agaaggttga tgattgatct agtttaaatt gtgtgaaatt tgaaatctgg tgtttttact
    11041 agatgaatca cctaacaact atgattataa cttatagaac ttctgttttg tacttggttt
    11101 tgttatagtt gttataaagt tttattttat tacagaacat ttttaa
//
LOCUS       DAZKKW010000084        11132 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.086, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000084 DAZKKW010000000
VERSION     DAZKKW010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11132
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.086"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..832)
                     /locus_tag="ACG8PO_002200"
     CDS             complement(<1..832)
                     /locus_tag="ACG8PO_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFQ0207496.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            complement(846..1643)
                     /locus_tag="ACG8PO_002201"
     CDS             complement(846..1643)
                     /locus_tag="ACG8PO_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFQ0207497.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNAAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            complement(1676..1960)
                     /locus_tag="ACG8PO_002202"
     CDS             complement(1676..1960)
                     /locus_tag="ACG8PO_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HFQ0207498.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            complement(1957..2958)
                     /locus_tag="ACG8PO_002203"
     CDS             complement(1957..2958)
                     /locus_tag="ACG8PO_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-isomer specific 2-hydroxyacid dehydrogenase
                     family protein"
                     /protein_id="HFQ0207499.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            complement(2960..3862)
                     /gene="gnd"
                     /locus_tag="ACG8PO_002204"
     CDS             complement(2960..3862)
                     /gene="gnd"
                     /locus_tag="ACG8PO_002204"
                     /EC_number="1.1.1.343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydrogenase
                     (NAD(+)-dependent, decarboxylating)"
                     /protein_id="HFQ0207500.1"
                     /translation="MEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDI
                     PAYQEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSI
                     RRAEEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLY
                     AGASGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWL
                     MEIMEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEED
                     SFSAKVVSALRNGFGGHEIVEKGE"
     gene            4026..4874
                     /locus_tag="ACG8PO_002205"
     CDS             4026..4874
                     /locus_tag="ACG8PO_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFQ0207501.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            5520..5726
                     /locus_tag="ACG8PO_002206"
     CDS             5520..5726
                     /locus_tag="ACG8PO_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HFQ0207502.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(5928..6929)
                     /locus_tag="ACG8PO_002207"
     CDS             complement(5928..6929)
                     /locus_tag="ACG8PO_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFQ0207503.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(6934..8846)
                     /locus_tag="ACG8PO_002208"
                     /pseudo
     CDS             complement(6934..8846)
                     /locus_tag="ACG8PO_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
     gene            complement(9014..9520)
                     /locus_tag="ACG8PO_002209"
     CDS             complement(9014..9520)
                     /locus_tag="ACG8PO_002209"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HFQ0207504.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            9680..10120
                     /locus_tag="ACG8PO_002210"
     CDS             9680..10120
                     /locus_tag="ACG8PO_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFQ0207505.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            complement(10146..>11132)
                     /locus_tag="ACG8PO_002211"
     CDS             complement(10146..>11132)
                     /locus_tag="ACG8PO_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0207506.1"
                     /translation="SSIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHS
                     ITYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTY
                     LEEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFG
                     TKKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCM
                     LVRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDY
                     PIDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
BASE COUNT         3211 a         2291 c         1757 g         3873 t
ORIGIN      
        1 aaaatccgtc atcatagtca tcttcgtcat caaaagatgc tgaattgtta ttgcttgtgg
       61 caggctgtac ttgtttccct ttttgcgact ggacaaagat caaagcgata ataatcccaa
      121 aaatggcata agtaaccatc gtaatatcta aagatttcaa aacaacactc ataaaataag
      181 caaggaagaa aaagaccaga taatcttttc gaccgataac gtatacagtt gtcgcgatac
      241 cgattgccgc taaccctcca cctactactt caagaatgtg gataacgact gttccctcta
      301 aagctttgat tacatcagcg atcaatggcg cccctaaata taaagcaatg aaaacaagcg
      361 gaacgaataa taaaaatgag gcaaccgttg gataaagaat aattgatcgg gccatagctt
      421 ttgtattcaa atcttctaca gctttgtctg tatatttacc taaaaaagta ttgatgaaaa
      481 aacgaaactg atacaagtaa cttccgagca atccgactgg caccgcaacg gcaatcgctg
      541 cttctggcga cagatcccct aataatgcga ctggtactgc gatagctgct gcaatagatg
      601 gttcagctgg cattgatcct cctggagaaa aaacccccat ataaatcatt tgcaaagcgg
      661 ctgtaacaat catcgcttgt gcaacttccc ccatgatcaa cccgacgact aatccagtca
      721 tcaaaggaga aaaacgtaag gtcaacgtgg ctcctccacc taaccagcgg gacataaccg
      781 cggctaccca taaagcaatc aacaaacttt gtactacact taatgtttcc atgtgtcctc
      841 ctccttcaga tctttctgat ataaccttcc agttccatca ttgactgtat cagtgatttc
      901 tgtgttacct ctataggaat caaatgcact ttctctttcg aatcaataaa ttcagccatt
      961 ttatctatta tctgtttatc tttcggaaat aatcccattt gttctagaga aaccggcagt
     1021 tctaattccc gatacaaagg atataactcg tctatttttt cccagcgacc ttctagtgcc
     1081 agttgataga aaatcccata cgctactttt tctccatgta gaaaacgatg gctttccgga
     1141 atatatttac tgatagcgtc atgcatagca tgtgctgccg catttcttgc gtatttatct
     1201 cctaatcctc caactaaacc agcaacagca aagataatct ctgaaagctg tttgaactcc
     1261 gatgtcagtt ttctttgtgc catatcttgg attgctttag cagaatgatt caaaatctct
     1321 tctttgcaga tcttcgcagt atcttgtgct agcttcaaaa atgattcatc ttgcaaaaaa
     1381 tcttgttcta agatgagctc agattcatac cattttgcta gtgtatctgc taacccggca
     1441 atgaaatatg gaacagggga atcaatcact aattggggat ctgttataaa aaaagcagct
     1501 tgtctgtaaa agtgttctgt tttcccttct gcttgtccat tctccttata catgacagca
     1561 agaggtgtcc atggagcaca attactagct aaagtaggga tgactccgaa atcgattctt
     1621 gcaagattgg cactataacc cactagatcg gtcagttttc ctccgccaac gccaactacg
     1681 aaatcgattt catttttttg atgattgtag ctattcgtct ggcttcagca taactacatt
     1741 ctccagaata acgatgataa aaaaattgaa tcgtttcgtc attcaaaaat actaatttag
     1801 gtagcgcctt ttcccatgat accgttccgt gggtgaccag tactcttttc gcgccgtatg
     1861 atttcaagag atcagaaact tttgaaatac caccttcttc atataaataa aattgagggc
     1921 ctgttttcac ttttaaaccg ttcaccattt tcttcatcat ccttttatat agttgctctt
     1981 tgtctggaca gaacgttcag gaagaaggtg ctgactgatc tgttcgacat ctctgacaca
     2041 tttttcaccc attgcttcta aacattccct tgtgtaggcc gatatatgtg gcgtaatcac
     2101 tacttgttca aacgctaaat acggatgatc ttgacgtccc ggttcttctt ctaaaacatc
     2161 tgtggcatat cctgcgatct ttccgctttt cagaccatta atcatcgctt cttcatcaac
     2221 tagagcgcct cttgcactat tagatagata aacattttct ttcatctgat cgacttcttt
     2281 atcagaaatc atgtgatagt tctcttctgt caaattcgca cataaacaaa tgatatctga
     2341 ttgttccagt aattcttgaa gttctacttt ttttgcacca aagctttcga tgtataaatc
     2401 tgatttgtaa ggatcataag ctagaacatc acagcgaaaa ccgttacgca aaatttcgat
     2461 tactccgctt ccggtatttc ctgcaccaat cacaccaact gttttgttaa acagcgtcct
     2521 accgacgaac tccgcccgat cttcccatcg atcgtccttg actcttgctt gtgcttgtgt
     2581 cgtgcgtcga agcatggaca acaaattagt tatattattt tctgctacag catttcgttc
     2641 aactaaagca ggtatgatag atacgattgt ctggtgttct tttgctgctt ttaaatcaat
     2701 attgttgtaa ccaataccat gtcgggaaat caatagcagt tcatctttgt tttcaaaaaa
     2761 atctcgtcga aaaaatggcg taacacttgc aataatgata ttgaatcctt taagtaactc
     2821 cgctagttct tttcctggaa tctcttgatc aacagtaaag aaatgaacgt ctccaactga
     2881 ctttaatctt tccaagtggt gtggaaagac tcttccgaaa ctgctggaat taacgattgc
     2941 tatatgataa tctggcattt tactcaccct tttcaactat ttcatgtccg ccaaacccat
     3001 ttcttagtgc tgaaactact tttgctgaaa aactatcttc ttcctgtgaa agattcctca
     3061 tgaacaaact agttgcaatt actggaactg gaacagccat ttctagcgct gtttcaaccg
     3121 tccattttgc ttcacctgat gcagaaacga ttccccgata attacttaaa ttgggatgtt
     3181 cgtttaactg atcttccatg atttccatca gccagccgcg aatcaccgaa ccatgattcc
     3241 aaacacgtgc gacttgttcc aagtcaaaag catactcact tttttgtaca agttggaatc
     3301 cttcaccgat tgcttgcatc attccatatt cgattccatt atggatcatt ttcaagaagt
     3361 gtccgcttcc ggatgcacca gcatacagat atcctctttc aattgctaca tctttgaata
     3421 attgttccac ggatagaaaa gtttctttat ctcctccaat cattaagcag gcttgatgtc
     3481 gtgctccttc gattcctcca gaagtcccac aatccaagta accgatttca tttttctgaa
     3541 actcttctgc tcgacggatt gaatctttat aataagagtt tccgccttca ataataatgt
     3601 ctcctggtcc gagttttgga aaaagttttc ttaacatgtt ttcggttatc tctccagcag
     3661 gaagcattaa ccagatgatt tttttctgct caaaagtagc tagaaattca gagatctctt
     3721 gataagcagg aatgtcattt tctcttgcct gactaacagc tgtacttgaa atgtcgagtc
     3781 cttgtacttt gtgatggtgc tcttttaagt ttaaagctaa attcaatccc atttttccca
     3841 aaccaattac accaatttcc atgatagcgc ctccatttct tataccctca ttatacaaaa
     3901 aaattttttt gtaaatagat tttacaaaaa tattttttgt ttgatttttt cctactctcc
     3961 ttgtatcttt aacattaatg agaacggaaa aaatgataat cttattatga aaatgaggaa
     4021 ttctcatgag cgtctccata agactacaaa tcaaaacaat gtacaacgac ttcagcccaa
     4081 aagaacaggc tatcgccgat tatattttag aaaatccaag caaagtaagt catagtccta
     4141 ttagcaattt agcaagtgaa ctgggcattg ctgactctac tttttttcaa tttacaaaaa
     4201 aacttggtta taatggattt aaagatttta aaatggccat gttgatccaa gaaaatgatc
     4261 tatctgctat ttctatccac gaaaatattc agaaaacaga tactgagctc actatggcgc
     4321 aaaaggtgtt cgagtctagt atttcaactt taaatgacac aaaaaagttg ttacaacaaa
     4381 aggatctaaa aaaagcggct actattatca atgaatctaa acgtgtctat tttttcggtg
     4441 ttggcggttc agagattgta gcaactgacg cttatcataa atttttgcgt tcccctatcg
     4501 caacttctca cagcacggat taccatattc aattgatgga agcttcttta ttgacggaaa
     4561 aagactgtgc tgttttgatt tctcacacag ggcaatcaaa agaaacaatc catattgctg
     4621 aaaccgtaag aaaaactgga gcaaaaacaa tcgttattac tagccaagct aattcttccc
     4681 ttgctaaact tggcgatgtc gtttttattt ctatttctga agaaacagaa ttccgatctg
     4741 aagctttagc ttcccgtatt tctcaactaa gcattttgga ctctctatat gtgattttga
     4801 tgttttacaa cgaaaaagaa tcacgtcgtt ctatcgctaa agttcgacat actatttctg
     4861 aaatcaaaga atagcaccat aacagaaaaa gcgaaaaatc cataaaacaa aaaaagccac
     4921 taccaaatat ttggtagtgg cttttttggt tctgcaaggg tggtacagaa catcaatagt
     4981 cggtgggtta gaacgtatgt catcgcatct ttggagaagg ttgtattggt attattcgag
     5041 ttacttgtta gatgcgataa caaagttagt atacagtagt aaaaaattta tcttttcttt
     5101 tttttattat ttttgtttct tattttctaa aaaattaaaa aaaattttgg tgaattaagt
     5161 atttccttga taacactggc ttttggcctc attttaggtt atcatttgta attttatttc
     5221 acagttgata tgattgttat gtaaaataaa aaagtttagg atacatcgaa tttttgggga
     5281 aattcttttg ggggtatcct aaatcttttc tattatattc ccatattttc tattattggt
     5341 ctagacgtat gatggtaaac tttgtaccta tctgattgct gaatcattaa ttgcttatca
     5401 ttagccatct ttgtttcaag ttcgctagat gatcatttta ttcacatcct aacaaacttg
     5461 tcacatttat agattcttaa tctatttatc atacaattat aaaaaaggag gtgctgctta
     5521 tgaaagctat ccatatttct ttgctttttt tcgctagtgc attgatttta tttgctattc
     5581 atctttgttc ccagccttat atcaataaca acggaatatt agtggaacca gcattctttt
     5641 gcttaccact cggctttttg tcccttcttg cctccgcagg cttcggattt gttgcttttt
     5701 ttaaacgatc aaaacaacaa atctgactta taaaatttac gtaaaagaga tcgtgacaat
     5761 aatcgtgtga tattcccaaa tttcgaataa aaggtgcccc ttgccatctc taactcctca
     5821 gtcacaagtt acaatattca taaaccatga aaagcttgtt tattgaaatt gctccttgtg
     5881 acttggagct aagtgacaga ttacaacctc ttttgcatca taagacttta ctccttgaaa
     5941 aacattacta acttttgata tgtttcttgc gcttgtttat tgctgaaatc aaactgatat
     6001 tcatggttca aaacagcatt tttctccgca taatctactt ttgtgacttt cacccccgct
     6061 ttctcaaata cagaagttac ttcttccatt tgtttcgtaa acgtatttgt atttccatcc
     6121 gttaagaatg tgttttgcgt ccaacgatcc gcatgtctga ccaaggctaa acttttgacc
     6181 ttctctgatt gttcaaagtc cttattatca aagtaagccc atccacattt ttcataaaga
     6241 aagctagaga ttttatcact tacttgagta agtgcagaag gatccaacag accagacaaa
     6301 gaaataaact tcgtaatctg cttgttctcc aaagtcggat caagatgatc agctttttta
     6361 atttcttcat tttgaagtgc taacaaatat tctccactaa tttgtgcacc tgccgaattt
     6421 ccaccaatcg aaatattcga ccaatctgct tgatctttta tctctttttt agctgcttga
     6481 atggcttcgt tcaattggat aactggtgta ggatgttttg cttccggtga tcgttcataa
     6541 tctatattta cgatgatttg ctgcgtgtct gaacatagat attccaaata atcacgacaa
     6601 tcatttttat cccctcctac atatgcgcct ccatgcatcc agaaaagaat cggtaatttt
     6661 ttcttttcca ctttcggtgt gtagatatcc atattattat ttttgtactt tgaaggatag
     6721 gatatttctt tttttatatc gatttgttcg attttttgct ggaaatcatc tggtcgggca
     6781 tatacttgtt ctttttcact tgtgtcaaat tgttttctaa caagcaagct aatcggcttt
     6841 ggtgtgattt gattaatcaa aaatacagtg actaaaataa ctacgatgat aactggtact
     6901 gcaatcacta ttcgcttgaa ccatttcatt catttaccct ctctttcttc gccaaatcca
     6961 tgtgataatc gtgctaacag ataaaaatcc acctgaataa atgagaagcg aatgtttctt
     7021 ttctccagtt ttcggcaaca ttcttttttt gcatctgtct ttttttgtac tttcactgtc
     7081 atctgaatcg gttgcgtatc atcacttgat tgatcctctc gaggttcatt ctttccctta
     7141 ttgtctgcat tctctgtcgg atcttgcttt tttggtggag ctactgggat ttttttgtaa
     7201 tagaaaataa tagttggtat cgtctcatta aaaatccctt cagtcggtcc ttcaacattt
     7261 gctaattcgt atccttcgaa tgtcttttgc tctgtcatat aattttctcc ataagctccg
     7321 taaagctgga tttcgtcctg gtctttgagt agacttttat tgttttcatc cacatacttt
     7381 acgtttatct gatcatggaa cactactttc aaaaagtccg ttacatgttt cgtgtaggca
     7441 gcatggtcaa cttcctgtgc ctttccatgc cctgctcctt ctactaataa aagatcttta
     7501 taccctcctg ataactcata taatttttgc gcgttagaca caggtactac atcatctgct
     7561 gttccatgga taatcaattt aggcaatgaa tctgacattc caatcgtagg tagagcagct
     7621 tttgtatcca aatgcatctc ctgatcaaaa tagtattgtt tcaaaaggcc cataaataaa
     7681 tcttcatgct cttgatcccc aatgatgtct aactgatttt ttatggcatc cggtagtgcg
     7741 ggggctactg tctggctgta cgtaaaacgt agttgttcat caattgatgc aaatccgcaa
     7801 ttttcaataa ttcctttgac attgacagga atatctttcg ctaatacaga catcatcgta
     7861 gctgctccca tcgaaccacc ataaagtaaa atcttttgag atggccgctc tcgaacttct
     7921 tgatttatcc agtaaaggac gtcatctgac tcatattgtc cataggtcac ataatcgcct
     7981 ccgctattac cagtcgctct ggaatcaaca aataataaat tatacccggc cttataaaaa
     8041 tcattgtatt cttctgtaac gatcccgtta ttccaattcc ctcgaaatcc gccatggatc
     8101 atgactgttt tatctgaacc ttgatcgaca taatatgcat atacatccat cattttgcca
     8161 tcttgtttgt ttagtacagt ttgatgaagt tgttttgtat ttggatagaa gcctgtgttt
     8221 tctgtaggcg ttgtcccatc atctgtacct ccaagcatcg acatcaacac actgtttttt
     8281 gcaaaagcga tatcgtacaa tagataagta gcaatcagat gtttgataat ctctttatct
     8341 acaccataat cattaacagc aagctcagtg actaataact ctattaattt tgacttgatg
     8401 tcttctgttg gtccattaag tatttctgtc atctgtttac cgaagtaatt caaatattct
     8461 ttaccattga aattttcacc tttaccgata tcatcatttt ctgtcccatt ctctttgtca
     8521 cttactttag cagaagagga ttcgtcgttt gacatttttg tagctgttgc ttcttctgtt
     8581 tgttggttat ctgtggtagc ggtcgtttct tgatctgtgc tagtaacaga tgtctcaacc
     8641 gttgtttcag tatttgtttc tgtttgtgaa gtactcgatt tcgtttcatt tccagcatca
     8701 gtagtttctg tggtttctac ctcccgagat tccgattcgg aagaaatgct ctctccatat
     8761 actgtccatt gcgggacgct tgtacttaca gcaaataaga tgaccaacgc tccctttaat
     8821 acttgttttg ttctcaagct attcactcct ttttatgttt ctaggagaat tatatgtaaa
     8881 cgcaaacatt ttttatacaa aaaataaatg gtttatccca taaaaataga acaggattat
     8941 tttctcattt tacgtaataa agcagaatgg ataaaccagt atccattctg ctttttttaa
     9001 acgaagaaac aacttattgc tccaaaaatg tcagaaatcg tttatcccaa attttctcgt
     9061 aaaaagcgat tgtttccttt gctttcataa aaggattatc ataagttgtc gttgctcctc
     9121 tacacatcat aagctggtat cctaatccat gagcaaactt cacagtcgta tctatacaat
     9181 actctgcttg tgccccacat aattcgatcg aatgacagtc caattcagtt aataaaacac
     9241 tcaaattagt cttataaaat gaattcgcat gggtttttct cacaaaataa ctgtgctcag
     9301 gaatagataa ttccgacaat agctcccatg gttttgtttg tggcactaat tgagcatctt
     9361 cgtgttgaac gaaaataact ggtttcccaa tcgtttcgta tgttttgatt cttttattca
     9421 ctaatgctgt cactgtttct aattcgtgta tcggcgaaga atcaaaacat accccttttt
     9481 gtaaatcgat cactactatt gcgtcggctg tgtttgacat acttgtgcct tctttctttt
     9541 ttcttgattt aatagtattt cttatctagc tagttccatt atacgtgaca acattttctt
     9601 attttacacc tctaaatggc acttttcgac tatttcttca tgttattatt tcataaatac
     9661 ttagctattg gaggaatgta tgatgattaa attgtataca ggttctggct gctcgtcttc
     9721 aagacgcgca aaacgttggc ttatcgtcca tgatataccg tttatcgaac agaacttaat
     9781 tcgtcaacca ctaaccaaaa atgaatttat ccacttacta aaattgacaa acaacggcgt
     9841 gaccgaaatg ctctctactc attctattag ttataaaaat cttgttcaca gaatagatga
     9901 actcagtttg agtgaactat atattgagat tcgtacatcc ccttctttat tgaaaaaacc
     9961 gattatctac gaccaatctg caaaactttt gactggattt agcgaagata aaatcaggat
    10021 gttcctttct aaaactaaaa gaaagcaaga gctcaacgaa ctcttgcttt ctggtcatct
    10081 tgtcgatttt aaagaaaata atctttttgc aatgtcttaa ttttggcata aacaatctgc
    10141 ttttcttaat taacgaaatc caatttttcc aatatggatc tatttttctc attcctgatt
    10201 tttatgattt cagctttcag acgcgcccaa tcattttttg taggttgtcc attccagtaa
    10261 aatatctgtg tattcgtttg atcatatgcc tttttgtcga taggataatc gctaatgaca
    10321 atatcaggta actgatcagc ggatgcacta tattttattg ggcgttcagt catagataat
    10381 atccttttct ttgcccataa ttcctgtgtt ttaccatact ttgattgaat atagaatatg
    10441 acaggttgtt cgtgttttat aaaaatatcc ctgactaaca tacagtattg aaaaatcaac
    10501 cgtctgttct tttcataata agtattaagc tctggaattc tgcgagctac cccactaaaa
    10561 aactgttgca tgattggata catcactgga aatatatgct gaaaatctct gtcttctgct
    10621 gattttccaa aagcatccac cttctttttt gtcccaaaaa cccttgaata caaatggatc
    10681 attgccaaat tgacatatag ataatttttt tctgaagttg taaaacaaat cttgaagttt
    10741 ttttcaaaat tttctacaat agacgattca atccagcccc aactttcttg tactttatct
    10801 ggagtatcca acaaatcttc ttccaaatag gtgttcatta caccaaacat aaaataaagg
    10861 taagcttgct ctgttggagt gatgtgattc atcgaccaga agtttgcttc aaaaaaaggc
    10921 tctagtatgt aatgcgtaaa ctcttctttt gttatcaatg gatgcacaaa tttgttcatc
    10981 tcttctggga catacgtaat cgagtggcct tgtttcattc gttgcaacga aattcttagt
    11041 aggctgatcc atttctttct atctatacta cgcgtatatg gcgcatgttt cgcaaatttt
    11101 tctaatagtt gacgtttatt ttttatactt ga
//
LOCUS       DAZKKW010000085        10940 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.087, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000085 DAZKKW010000000
VERSION     DAZKKW010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10940
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.087"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(112..795)
                     /locus_tag="ACG8PO_002212"
     CDS             complement(112..795)
                     /locus_tag="ACG8PO_002212"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFQ0207507.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            complement(904..1362)
                     /locus_tag="ACG8PO_002213"
     CDS             complement(904..1362)
                     /locus_tag="ACG8PO_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HFQ0207508.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            complement(1446..3209)
                     /locus_tag="ACG8PO_002214"
     CDS             complement(1446..3209)
                     /locus_tag="ACG8PO_002214"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFQ0207509.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            complement(3430..4890)
                     /locus_tag="ACG8PO_002215"
     CDS             complement(3430..4890)
                     /locus_tag="ACG8PO_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0207510.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            complement(5098..6024)
                     /gene="glsA"
                     /locus_tag="ACG8PO_002216"
     CDS             complement(5098..6024)
                     /gene="glsA"
                     /locus_tag="ACG8PO_002216"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HFQ0207511.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(6047..7474)
                     /locus_tag="ACG8PO_002217"
     CDS             complement(6047..7474)
                     /locus_tag="ACG8PO_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFQ0207512.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            complement(7505..8719)
                     /locus_tag="ACG8PO_002218"
     CDS             complement(7505..8719)
                     /locus_tag="ACG8PO_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HFQ0207513.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
     gene            complement(8805..9032)
                     /locus_tag="ACG8PO_002219"
     CDS             complement(8805..9032)
                     /locus_tag="ACG8PO_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0207514.1"
                     /translation="MKNKFENRSDTRCGSFLYILYEVFFIPYEKISLKIKKDENKRFS
                     IKRRPFIFCLYLCEKRKRAQHIKLLLIVCLL"
     gene            complement(9060..9302)
                     /locus_tag="ACG8PO_002220"
     CDS             complement(9060..9302)
                     /locus_tag="ACG8PO_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFQ0207515.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
     gene            complement(9334..9891)
                     /locus_tag="ACG8PO_002221"
     CDS             complement(9334..9891)
                     /locus_tag="ACG8PO_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFQ0207516.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            complement(9904..10092)
                     /locus_tag="ACG8PO_002222"
     CDS             complement(9904..10092)
                     /locus_tag="ACG8PO_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HFQ0207517.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            complement(10105..10671)
                     /gene="amaP"
                     /locus_tag="ACG8PO_002223"
     CDS             complement(10105..10671)
                     /gene="amaP"
                     /locus_tag="ACG8PO_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HFQ0207518.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
BASE COUNT         3237 a         2278 c         1789 g         3636 t
ORIGIN      
        1 cagatcttga agacaaaatg aaaagtaatg cttttttcat cttcttagaa tctgctttaa
       61 actatctatc aatttttctt ttttgtcaag aatctgtgcc gtgcgtgttt tttagttatt
      121 tgctttactt gcggcttcga catatctttt agtgatcaat tgcggacggg tgataataga
      181 tccaactacg actgaaaaag cacctaattc atatgctttc tctacttgtt caggagagtc
      241 agtatgtcct tccataatta ctgggatatc gacttgttct atgatttgat tcaataaatc
      301 aaaatcaggt gtctgcgtct ttcgtgaata ttctgtatag cctgctaaag tcgtagacaa
      361 aagatcaaaa ccatcttctt gcgcttgaag cgcttcctca aaagtcgaaa tatctgccat
      421 agctaaccga ccgtgttcgt gaatcatttg gacaagttca gcagttgttt tttctgtcgg
      481 acgtttacga gaagttgcat ccagtgcaat gatttctgct tgcgtctcca aaagttcttc
      541 cacttctttt tctgtcgcag taataaacac ttctgaccca tcgtaatctc ttttgataat
      601 ccctattacc ggtaaatcaa cgattcgctt gatttcattg atatcttctg tcgtatttgc
      661 tcgaatacca actgctccgc cttcttttgc agccaaagcc attctcccca taataaaaga
      721 actgtgtagc ggctcgtttt ccaaagcttg acaagaaacg acgactccgc ctttgatttg
      781 ttccagtgta tccacaatat ctcctccagt aatgttatat aaaaatacag cttcatataa
      841 aaatgtaacc cttttctatt caggagacaa taatgagcta tttctttcag cagacctttc
      901 ttactaaaag aaaattactt caaattcttc gtatacgacc agcttctctt ctgtaaattc
      961 tatgttcaac gcttctttat aagcatgttt aaaaaactca gcatggactt tccgccaata
     1021 agctaatgtc cgatcccctt ctccttcttt ccaagcatgc tctttcgtga cttcactaaa
     1081 aggcaagatg gtcacattta tatttttgat gatacaaact ggttcatttt ttccattcag
     1141 gaccacgtct aattgaccaa cttttggtaa aggctcattc tcttgtttat acagatcgta
     1201 accacttgat gtaccagtct ttatcttttt tacaactagt tcgctcaact cgtctgccat
     1261 tttttctgta tccccaaatg aaaagaaacc taatttttct gattgataac taggatattt
     1321 ttcttggaac tttttgacat attcttctac ttgctgatac ataacatcca cccctttgct
     1381 ttttataatg aaaaaaccat ccagatacta gtataaacaa ttatctggat ggtgagcata
     1441 atgaattatt gaatattcaa gacacgcaga ctatcttcaa cgatttgttt tggtgttaaa
     1501 tgataacgtt cataaagctg ccctacagga acgctatccg tgaattcttt ctctgcacca
     1561 aaatttaata cgttcattga agaatcacca taataacgac tgatattttc gccaaatcct
     1621 cctgacaaac taccatcttc taatgtgatg actaattcgt gtgtttcttt taatttatct
     1681 aatgtttcta cgtcattttc tgtgatcgtt cctggattga tcaacgttgc ttcgattcct
     1741 ttttttctaa aagcttttac tgtttctttt cctaattcaa gaaaaccgcc taaagctaaa
     1801 attgctactt ttcctccatt atttactgtt tgataggctg gaatactata atcatttgcg
     1861 atagctgtac catgttctac accatgtgta ggaattcgga ttgctacagg ctgcgctgtt
     1921 tgttcgaacg cccaatctag catagagatc atatcctctt ttgttgctgg agcaagatat
     1981 ttgatgttcg ggatacttga taagtaagga atgtcaaatg ttccttggtg tgttcggtca
     2041 ctagctgaga tcgttccgcc accgactaaa ataatcactg gattctcgtt gatcgctaaa
     2101 tcatgtgata attgatcata tgcccgttgc aagaaagtac ttgattggaa gacgactgga
     2161 cgtactccct gagaagccaa agcagaagcg aaagtgatag agtgctgttc agcgatcccc
     2221 acgtcaaaat aatgatctgg atgtttttct tggaatgttt tcagtccaaa ggcgcctggt
     2281 atcgctgcat tgactgcggc aataggagct gtacttgttt gcattttctt atccaaataa
     2341 tccatgatga tcatattaaa tgtttctcca gaagttactt gctttgcctc cccggtggac
     2401 agatcaaatg gtacatgcca atgatatttc attttattat cgattgccgg ctgataaccg
     2461 cgaccttttt ccgtatggac gtgaagtaca atcggatgat caatatcttt tacttcttca
     2521 aataaacgga tcaccgtttc cagatcgttt ccattatcca cgtaacgata atcaaatccc
     2581 attgctttaa ataggttatt gctgctttta ccgttggttt gacgaagctc agctaagttt
     2641 ttatataaac tgccatgatt ctcatcaata gacatctcat tatcgtttac taatacgatt
     2701 agattggatt gtaaaacacc accattgttc aatccttcaa aagctaagcc accacttagg
     2761 gagccgtcac cgataaatgc taccacattt cctgattctt tcttcagatc tctcgcttta
     2821 gctaaacctg ttgctaagct gatagatgta gatgtgtgcc ccactgtaaa atagtcatgt
     2881 ttactttcat gttggttgga atatccgctc acttcacggt aatgtccttt ttcaaaacca
     2941 ttttttcgac cagttaaaag cttatgacca taagactgat gggaaacatc ccaaacgatt
     3001 ttatcctttg gtgaatcaaa tacataatga aacgcaatcg ttaattctac tacacctaga
     3061 ttcggaccaa cgtgcccacc tacatcgctg tccttttgca agattttttg gcgcatctct
     3121 tctgccaact gtttcatttc ctcaacagat aattttttga catctgcggg gctattgatt
     3181 gcatccaata catgtaatac tgtactcata atacattctc tcctccgtac tatcttcttt
     3241 taatgaaaat agatttgtta cactttatct attctactac ttagagttga ctctaagtca
     3301 atagcatttt aaaaaaattt cagagaaagc ttgttatata agtagatctt cgcacgaatc
     3361 aggactcaaa taaaaacctt ctacagacaa ttaaaaagct ttgtagaagg tctcctaatt
     3421 aaggtctcat caagaaatat gtttttttaa taggtgaagt tcctgagcaa tatgatacaa
     3481 atggatgttt gcacctttta atgagattgg atagatcttt gtcttttcag tttctaatcc
     3541 attttgatag ctagcagcat cgcacaaaac cactcttttt ttcgcacata gacattcatc
     3601 aggcaattct tctaatgaac gaaataaaat aggatttatt tcagcgatgc cttcttccgc
     3661 atacaaatga aattgggttc gataaaaaaa ttgtttgact gaactttctg aaacaacaaa
     3721 aatttctatt tcctgtctcc ccgcttgttc actgttgatc aaagaaagac ttttgacgaa
     3781 ttttcgaatg aaattttcat tccatcttag ttggatttga tttttctttt tccactcttc
     3841 caatatttct acaatttttc cgtacagaag ttgttctttt tctgatagaa gatagatcct
     3901 ttccggtaaa aatacttgtc catctgccca caacgtacgg acaaattgta aaaaggattg
     3961 cttgaaaata aaattttctg catcgtcgat ttcagcgata ctaataatct gtaaaatcaa
     4021 ttcattcagt gataggtcat tgagaacaaa atttttataa acaacttcga aatcttttct
     4081 taacagctcc atgttctcat tggtataatt tctactgttg aacaaagaaa aaatataata
     4141 gacatcttct tcttgtaagt ctagaattcc tttttgctct tccaatcctt gacagactaa
     4201 ttgaaataac ggcttcgttt ctgcgctttt cttatcttct tctttcagtc caaggggaaa
     4261 tgcctttctt ccgattgcga tctctattcc tcgtatgata aaggactttt catcttccga
     4321 atatcttctt ttaaaaaaat gagtctctac ataatcaatc aactcgttac aagcttcttc
     4381 tacatacgga tttttatttt catagccttc ccaattgaca taacgtacta aagctagcaa
     4441 gatgttccgc gagtcaaatt ctgggataat gatttcttta tttttcaaat aatcattttc
     4501 tttaaaaaaa tccaatacga tttttttgat tgtatatact ttgctcaaag aaataaattc
     4561 aagatcagcc agttctgtac tcgtgaatat accattaaaa taataactta aaaactttaa
     4621 aaaataagat tgaccgtaca gaaactgtgt gagctcagaa agagcaatac catgtttgaa
     4681 tacgagttta tacttatgct tctctcttac ttgttctttt atacaatatt ctagacactc
     4741 ggctagactt tctagatcgt gaaggatcgt tggtgtagtc acccttagag aaatagctgt
     4801 ttgttcgatg tctatttctt ggaattcaaa taattgttca cacagataca cttttctaaa
     4861 tatatttcgc tcgatgtaat tttccagcat tcctgataca cgcctccagt ttttcttatg
     4921 attttattta atcatatctg taaatcgtat ttttgtttcc tttttattta gaaaagccct
     4981 ttcaaaaacc aataaaaaaa gaaactggga caaaagtcaa acaagctcac aatttcagcc
     5041 tcattgtagg tagctttgag ccgtttggac ttttacccca gcctctttca agaatggtta
     5101 ttcaaagatg tcagcgtcta gttctttgac tacatctttc aacaattgga taccagctgc
     5161 gctgttacca aatggatcta gtgccgggct gaagacacct atgccgtaac gatttggcac
     5221 agctgccatc aacccgccgc ctacaccgct ttttgcaggt acaccaacat ggacggaaaa
     5281 ctctcctgat tcatcataca aaccagcagt tgtcataatt gatttgacga ttgtagcact
     5341 ttcttcagta atgatttgtt tgccattcca aggagcaatt cctttatttg ccagcacagc
     5401 agctaatttt gctatgcctg tagcggtgac attgagggag cattgtctga aataagcatc
     5461 gagtatctcc ggtacgtctc cttcgatcat ttgattacct ttcatatagt aagcaagaga
     5521 gcgattgata tcacctgttc ttgattcaga atgatagatt tcttcattca atgtgatatt
     5581 aggatcatca cagatctctt tcataaatga caagattcgc tcaaattttt catctgcatc
     5641 tttaccggca atcaacgagg ttgttgcaat cgctcctgca ttaacaaaag gattcattgg
     5701 atgtttgcga tgattgattt ccatattcat gatagaattg aaggcaaaac cggttggttc
     5761 cgtgttgatt gtttgaaaga ctttttctat tccgttgtct ttgattgcta ataacaatac
     5821 gggaactttt gacatgcttt cgatggcaaa gcgcacatcg gcgtctcctc cctcaatcag
     5881 ttgttttgta tccaaatcat aaatcgccat acctaattgt ttgggatcca cttcagctaa
     5941 tgctggaatg taatccgcca cttgtcctgt ttgataatag tggaaatttt ttccaatcaa
     6001 ttgttctaat tcttttactt ccatttgaat aaccagcttt cattttttat ttttctgcat
     6061 gctggaattt atgacggcat gcgtagatcg taaatggaat aactaagacg ataagataag
     6121 aaacaatcag aatcgtttca taaatcccat gctgtccagc tgcgatacta gcaggtggaa
     6181 caaatgagat acaaaaagca aaaagtgaag tcaaaagacc taaacaagca acgattactt
     6241 ttacaacttt tccgcctgga acctgatagg ctctttcaag actgtcatct ttaaaaacca
     6301 gcttcaaata gcccgcgaaa aacataaagt acaacatcag gtagattaca acagtcaatg
     6361 acatcgctgc aagaaatgaa agattattcc ccccgccgct taatgtaaag acggctgccc
     6421 agattgttgc aacgatccca tttgcgatca tcaaatgagc aggaacaccg tgtgtgtttt
     6481 cttttgcaaa atattcagga agtaatcctt cttctgctgc tacgtgaagt gcttttgaag
     6541 gagaggttac ccaagaagca atttctccga tgacacctaa acaaacaaga atcgcaagaa
     6601 ttttcgcaat ccatgcaaga tttggatcaa tgtgtcttaa taggaattct aacgattgaa
     6661 tcacccctgt attaagagac aaaccatgtt gaggtactgt agtagcgacg gtcaatccgc
     6721 caaaagtatc taaaatgatt gccagaataa ccaatacaaa cattgctaat ggataatcct
     6781 ttttagggtt ttccatttca tttgcatgac tggcagatgt ttctacgccc atatatgcta
     6841 aaataaagga aacaaaaaca accagcgtag gcaatttcga aaaatcagga atcaatgttt
     6901 tcatagacag tatcgtttgt agtggagcgc ctgaagcgac ataatggatc cctaagccaa
     6961 acaagactaa tgctggcaac aagataccag ctacaaaacc gatccgagca atcaatgaag
     7021 tgtatttggt tccttttaac tgtgagagta aaatagccca gaaaataatc aataggattg
     7081 ccaatttcag atatttattg ttttgtatag caccaatacc cgttgcatag gataatgcac
     7141 cagttaagaa atacagcatg gtcaagtagc caactgttat ctcaaaccat tggaaaaaga
     7201 ttgcggcaaa accgaatcgt tttcccaatg tgcgactgac ccaagtatag acaccgcctt
     7261 tttcccatcc tttcaccgtt gccatctctg ctgcgcacaa tgccacaggg ataaaccata
     7321 gcaaaccgcc taataacaag aaaaatacta agctgaagcc tgaggtcgcg aatgtgggat
     7381 attcatataa tgaaacgacc attgagatgg tcatagccaa aaagccaaag aacgttaatt
     7441 tttttgcagg aattttcgtt ttgctttgtt gcatgaatct tcctcttttc tatttgaatt
     7501 tgtttcaatt gagtttgttg ccaaagaaga aaaagtccaa tccttcttct tgttcttctt
     7561 ttgtagtagt gatcgttgta aagtgcatcg ccgattttaa aatcactgtt gtcagcacat
     7621 aggcatacac ggcagtaatg atgacaccaa tcacttgaat ccctaagaag taccagctgc
     7681 ttggattcgt tagtaaatgg ctgttagatg caaagatccc tatcagaatc gtacctgtga
     7741 atccgcccat gccatgaaca ccccacacat caagtgcatc atcccattgt ttctttttac
     7801 gaaaatcaac agcagcatga cagacgatac ccgcaatgat cccaatcagc atggcacttt
     7861 ttgcttctac ataaccggca caaggcgtaa tggctgccaa tccagcaacg gatcctgtta
     7921 gtacgtcgac aaaatcaaag ccatttcctt ttacttttgc aatgatcagc caagtaatca
     7981 tcgcaaatgc aagagcgatg aatgttgaac caaatgcatt gactgcttgt tcatctgctc
     8041 gaagtgcacc accggaatta aaaccgaacc agccaaacca aagcagtcct gtaccgatag
     8101 cagctgccat aagactgcta tgctgacttc tttctcctaa aggaatcttt ctttgaccaa
     8161 gtgtgaggat acatgctaaa gagccgaatc cagctgtcgt gtggatgact gctccaccag
     8221 cgaaatctct aaatcccatc tgatccaaaa atccgccacc ccatatccag tggcaaacag
     8281 ggaaataaat cagcagattc caaaaaataa caagtataat atagcctttt aaattcaatc
     8341 tccctgcaaa agctcctgtc atcaaaggaa cggtaatcac gataaacatc agttggtaaa
     8401 gaaagaacat taaaaatgga atcgttgcac catgaaacat gttggggaag cttgtaacat
     8461 gtctcatcaa aaagaaatct gccgggtttc caataacacc gccgatatcc cgtccaaagg
     8521 ccaatccgaa tccaccaaat acccacataa tcgttgttat cccaacggag agaaaagatt
     8581 gagacatgat cgttagtata ttattcttcc gtgctaatcc gccgtaaaaa aaagctagac
     8641 caggagtcat caggcaaacc attgctgtac ataaaatcat gaatgctgta tctcctgtat
     8701 tgataggtaa tgtattcatt tttttcgcct cctttctacg attacgagta taaaagaagg
     8761 tgtagttgtt tttttcttct gtttttttga attaatactt gcatttataa aagacagaca
     8821 attaataaaa gcttgatgtg ttgtgctctt tttcgttttt cacacaaata aagacaaaaa
     8881 ataaacggtc ttctttttat agaaaaccgt ttattttcgt ctttttttat ttttaaagat
     8941 attttttcat aaggaataaa gaatacttca tataaaatat ataaaaaaga accgcagcga
     9001 gtgtcgctac ggttctcaaa tttgttcttc atttcaacat aagtttttct tcaatttgct
     9061 tataatttac gtgcaaagaa tgaaacgact aatactaaaa tgattgcacc gatcaatgat
     9121 ggaatgatag ccattcctgc aattgttggt ccccaagttc ctagtaaagc ttgtccaatt
     9181 gctgaaccta ctaaaccagc aatgatgttt gcgatgatcc ccattgatga tcctttattc
     9241 gtgattgctc ctgcaattgc tccaatgata ccgccgacga ttaatgccca aataaatccc
     9301 attttaaatt cctcctaact gacttcttac ttcttattgt acacgtggtt cgttattttc
     9361 tttgactttt tgtgcacctt tgcctacagc agattttgct ttatctgttt ggcgtgaagc
     9421 aaattgtcct gtggattttg ctgcttctgt tactttgtct tgtactgttt cttgatcttt
     9481 ttggtattct tcttgtgttt tgatgtctgc aacattcaca ttcacttcga taacatctaa
     9541 atgtgtcata ttgtttactt cagtactgat caattctttg atttggttgt aaatatcttc
     9601 gatgtcttta ccgtattcta cgatcacatc tagatccaca gctacttgtt tcttacctac
     9661 ttctgtattg atacctgctg ttacgttatc tgtgttgact aatttttcag ccatatttga
     9721 gaaaaatccg ccatctaatg tcaatagacc atctacatct ttcaatgcat aaccgatgat
     9781 tttttggatg actttgtctt cataagtcaa ttcaccattc atcatgcggt tttctccttt
     9841 ttcatttgcc aaaactgatc catttgttgt attcatgttt ttgttttcca tctttgtttc
     9901 ctcctatgat ttaaaaaatt gatcaactat tcctgactgt ttcaaataaa aaccgattcc
     9961 tgcgccaatt gctgtcatga ttatgatcaa caatgtcttg aaaaatccaa tcgttaacca
    10021 taaaacagct agcagtagtc caagaccccc aaagataata gggagcttgt atgctgaaaa
    10081 tagttctttc attatttggc cctcctattc cactcgagag tgcttgtagt ctcttgtatc
    10141 ttcttgttct tcataaccgc tgtatgtgac tgccactttg accgtttctt ttgttccgag
    10201 tactttagtt acttcttctt cgatatcttg catcaataca cctgttttcc ctactaatga
    10261 agaagttcgt ttcaagtctc cgttgacatg gatatggatt cgatttttcg ttgaacgtat
    10321 ccgtacttta ggtgaatgaa tgaactcttc ttcgtgtaaa tgggaacgta ccaacccttc
    10381 gatcgctttt ttgtcgattg ttagtttgcc atcttcccgt ttcataacga aagttcctct
    10441 tgactttgga tagaaaagaa cgactagcaa gatgataata gcaagaacgg caaatactac
    10501 agctgcccag aaaatatatt gttgcatgta gtaattcgtt aaagtaaaga agcgaaatcg
    10561 ttccaattgt tcaggcataa tatacggcgc attgatcaac gcaatcgcta gaaatactga
    10621 aagtagcagt aaactcatga taacaccgat tatttttaac cccttattca ttttccacct
    10681 cctataaact tatttttacc ctgtaaataa ctctaccgac aatcacagta tagatagcga
    10741 acagttagca tagcatcagt aaataaagcg ctaccaaaca ttgctggatc ataacggcgt
    10801 tttattgcca gtctgatact gaaataacaa ttgcttactt attctctatt tacttttgat
    10861 tgctggctat acttaaagga tattattcaa gattaaaaat tgcaaagatt atgctcagtt
    10921 attattacaa gattctattt
//
LOCUS       DAZKKW010000086        10667 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.088, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000086 DAZKKW010000000
VERSION     DAZKKW010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10667
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.088"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            339..2285
                     /locus_tag="ACG8PO_002224"
     CDS             339..2285
                     /locus_tag="ACG8PO_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFQ0207519.1"
                     /translation="MIERHVNLLKLLSRQEDFKPASFFSDKLAVSTKTIYQDIELLED
                     YVVNYQVTIERLPSLGIKLIGDRKNVEHLISSVENYLEKDIYSPEKRRVEIIKEVLFG
                     DKPVSLTHLSQSFMVSKTSLYNDLRIINKIIGSGEVKLQSSAEGISFTGNEQAIQRAI
                     KQLVFYYANDMESKTFFQVLNMLFDSKVISVIHDLLFNDYSELTEKVSDYYVRSLIST
                     LVIQCNRLLKGHHLTVEEEFLFNSIRYMETYIVANGLIEELKAKLNLPFYDADKEYLC
                     RQLFAHRVTNNLKTSNEEYAMSVHKLIERMSEIEKIDLTQNDRLYQSLLYHVPAMVLR
                     LKKGIKIQNPLLENIKSQYSELFSIVWYAMSILEEEFDVTLTDDEVSLILIHFQIAIE
                     DQSKANNIIIICQYGISSAQLIYNKVRRFLPAKDNVEISTLDKFHLAEIGEVDLIISS
                     LSFDAGGIPLVRVSPLVSKTDYMNIMEAYTKYLIEEPSRFIQNELIPKAVKVPTISKF
                     VQSSLVKTGVQVESKEECLDLMISELEANSLVTEEFRASIFSREKMGNTNLESGVALP
                     HANPSMVIESNIFIVALKNAINWGDRKVKLVIMVSLAEEDVSEIRGVYEELYQFIGQK
                     EMVDRLLQVHDSEKLLRIFQQKGE"
     gene            2288..2743
                     /locus_tag="ACG8PO_002225"
     CDS             2288..2743
                     /locus_tag="ACG8PO_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFQ0207520.1"
                     /translation="MYLDKELCLFQLEVKDQKELFQVMSEQLKNAGCVKDSFLEGITN
                     REQEFPTGLEVNQIGFAIPHTDSAHVNSSQICFASLKEPLVFSDMTDKSHEIPVRLVF
                     MLAMSQPHEQIDTLQNLVSLFQNEEKVNELLACTTKEAFIELVHSAGVY"
     gene            2757..4085
                     /locus_tag="ACG8PO_002226"
     CDS             2757..4085
                     /locus_tag="ACG8PO_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HFQ0207521.1"
                     /translation="MDIVLNAFEWFIGLGSNVFLPLIIFVIGILFGLKPGKAFVSGVT
                     VGIGSIGLGLVLSLLSDGLGTAIQQMGEQYGGGLNILDIGVGVGGPLAYSTSLGILMI
                     PISLILNFIFVVLGWTKTLNVDIWNFWFAIFLGMITQSVTGNFWFGLIGAIVAVILQW
                     FLADRTQKTVSEFFGYPGISITHMMGLSGVLFAKPVNWVFDRIPGFNKIDADAETLTK
                     RFGVLGDTVVIGLIIGIVVGIMAGYDVAGIGNLGIMTAAVMKIMPKMVAMFMEGLMPI
                     AEAAKEFTNKKLNGRQVNIGMDAALTVGHPTIMSTSLLLVPISLLLALILPGNKVLPF
                     GDLAFFAFAICLMIPYFKGNIVRSLVGCSLYVVTTLYMATWLAPIITDVFTLASFDVG
                     TAGLVTMLLCGLWPVGLFVLLASYFGVIGIGGLGVVLVGASIYMNKVKKA"
     gene            4119..4403
                     /locus_tag="ACG8PO_002227"
     CDS             4119..4403
                     /locus_tag="ACG8PO_002227"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0207522.1"
                     /translation="MKKLLVICGAGHATSTIAVAKLSKWLEEEGLTDQVKIYQSKIAD
                     ELNKIDEYDAVVSTTIVPDVIKAKVTNGLGLLTGIGTDAIYADVKERLGV"
     gene            4405..4920
                     /locus_tag="ACG8PO_002228"
     CDS             4405..4920
                     /locus_tag="ACG8PO_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HFQ0207523.1"
                     /translation="MRFHHDTLTIETVSGRPSYHDIRKELLDTVDTAGIENGMLVISS
                     PHTTCSLFFEETMHDTNFFGDDYLHVDINNVMETIIPKMTSENQYNSPGPKHIDFGTR
                     LSDPNYPAEKWVMLNTDAHLRSSIYGTNSMTLIVQEGKLLLGEMGRVYFVDWDQLRER
                     TRTINLMIMGE"
     gene            4936..5598
                     /gene="rpe"
                     /locus_tag="ACG8PO_002229"
     CDS             4936..5598
                     /gene="rpe"
                     /locus_tag="ACG8PO_002229"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFQ0207524.1"
                     /translation="MTIAPSLLNAPIAEVKELFEILKEKNVDYLHIDVMDGCFVPDMA
                     FGPSVIKDIRPLTDVVFDVHLMIDTPEKIVTRFVEAGADIVTFHIEATKHAMKVIQLV
                     KEAGAKVGITFNPATPVSFIEPVLSLVDQVLVMTINPGTENKHFIQETVVKIEQLDVI
                     RKQNDYTYDIEVDGKIDNQTIKVCSKAGADIFVSGGFIFNGKNTPQEQIDKLNQSLEE
                     VK"
     gene            5605..6177
                     /gene="hxlB"
                     /locus_tag="ACG8PO_002230"
     CDS             5605..6177
                     /gene="hxlB"
                     /locus_tag="ACG8PO_002230"
                     /EC_number="5.3.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0043800 - hexulose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-3-hexuloisomerase"
                     /protein_id="HFQ0207525.1"
                     /translation="MTNKDQNKNYLEAIILELQNNSKRINSVELEQFSEKIVQAKHIF
                     LAGAGRSGIAMQAFTNRLMHLGFSVSLVGEISSPHSQPGDLLIICSGSGETGSLKNLA
                     AKANQSGVSIALVTMKKDSSIGQLADSVLVLPGITKDENQREEDTFSQPMGSSFEQLA
                     FLTFDSLVLSLMDKTGESSETMFARHADFE"
     gene            complement(6364..7884)
                     /locus_tag="ACG8PO_002231"
     CDS             complement(6364..7884)
                     /locus_tag="ACG8PO_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HFQ0207526.1"
                     /translation="MTKMESELLVQIKQLTQLLEIQQQENTLLREQIAEMNRRLFGRK
                     KETPPVDGQIDLLDDSTFNEPEHTGQESQEPITVSSFKRRKRKGLKALSLEGLPEVPI
                     HYELKGADCLCEDCGHTLHDIGATRLREEPLYVPAHIEKQVIYQHAYHCRECEALGET
                     KIKKAPVPKPLINNSLGSSSIVTQSIWEKFVKKVPAYRQEKEWHALGFPLDRKKITNW
                     HIKVSEMVLEPIYNCLHQELSNQEVLHADETSYRVLNTHKEKTFYWLYASGKSEEKQV
                     VLYEHANSRSVDEPKSFLESFSGFLHTDGYAAYGKLPVNNVYCWAHVRRKFFEALGKQ
                     PSPKSHAKIGLDFCDKMFRIERTFNKLSAEERFVARKETLQPVMTEFFHWCESFAYLP
                     KSQLGKAVQYAVNHKDGLQIVLLDGRLELSNNRAERAIKELVIGRKNWLFSKSLKGAR
                     SNGIILSIIQTAVANGLNIRKYLNHLFTEIPNLSSMTPEALRAYLPWNQQIQEICK"
     gene            complement(8127..8312)
                     /gene="tnpB"
                     /locus_tag="ACG8PO_002232"
     CDS             complement(8127..8312)
                     /gene="tnpB"
                     /locus_tag="ACG8PO_002232"
                     /inference="COORDINATES: protein motif:HMM:NF017528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFQ0207527.1"
                     /translation="MPMLIDATALKHIFIVCGKTDLRKGIDGLAAFVIEEYDLDVYDQ
                     ALFLFCGTKKDRFKALY"
     gene            complement(8296..8478)
                     /locus_tag="ACG8PO_002233"
     CDS             complement(8296..8478)
                     /locus_tag="ACG8PO_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0207528.1"
                     /translation="MNKKDIVPVQFSSAAQKSQIDTKPKLACRIKSEDKEIFIYNGMD
                     KYLLYTLLKELNANAH"
     gene            8719..8937
                     /locus_tag="ACG8PO_002234"
                     /pseudo
     CDS             8719..8937
                     /locus_tag="ACG8PO_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            9432..10031
                     /locus_tag="ACG8PO_002235"
     CDS             9432..10031
                     /locus_tag="ACG8PO_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016622858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0207529.1"
                     /translation="MKTVEPIRDKKKIDAMKAILASGKYGQRNLVLFSIGINTAYRIS
                     DLRQLKLADVLEISRGRVVVKDRLAMKEQKTAKHNSVYISNKLRKTILEYVQQEFPDQ
                     LLQQDFDGYLFPSRKGTNEPLNRQSLWRIIHDAGTAIGLKDIGPHSMRKTFGYFLYKQ
                     GTKTEIIQSLLNHSSQRETLRYIGITQEDKDTAVKSLDL"
     gene            10095..>10667
                     /locus_tag="ACG8PO_002236"
     CDS             10095..>10667
                     /locus_tag="ACG8PO_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbiH family protein"
                     /protein_id="HFQ0207530.1"
                     /translation="MSTLYIIGNGFDLSHGLKTRYTDFSKWVEKSHSEIFDKIDTMIN
                     ENNEVRSTADDKIGWFNFESSLHELIQSPVFEDYVEESKDYLIGYGHEDWSDSAHHDF
                     QNMVYDYVVSLEELQQLFKEWIRKEIQPTIEHKIPKYQFSSDVLFFTFNYTDVLEILY
                     KQPKENIYHIHGSYDDIIIGHSENFDNFIGD"
BASE COUNT         3528 a         1679 c         1990 g         3470 t
ORIGIN      
        1 atgtttagac attttcctct aggaaaaaat taactagcac cacgggtata ttataataaa
       61 aaaatacgaa ttttagtgac gccattatat tgtttttatt tttcaattgt aatatttcat
      121 attacttcca taataaggag tattttctat tggaatatct tagctctttt aaggatttct
      181 ccttttttgt ttctcatttt ttaagattat ttggatttcc tttacaacaa acttccacaa
      241 gtattattga catagatgta attgagggaa gggggttcca gtgctagaat aaataatgaa
      301 aagaggtaac aaatgctagc gttaagaggt gaacaagttt gattgaaaga catgtaaatt
      361 tattaaaatt attaagtcga caagaagact ttaaacccgc ttcttttttt agtgataaat
      421 tggcagtgtc gaccaagacc atttatcaag atattgaatt gttggaagat tatgtagtta
      481 attaccaagt aacaattgag cgattacctt ctttgggtat caaactcata ggtgatagga
      541 aaaacgttga acatttgatt tcatcggtag aaaattatct tgaaaaagat atttattctc
      601 cagaaaaaag acgtgtcgag atcattaaag aagttttgtt tggtgataag cctgtttctt
      661 taactcattt gtctcaaagt tttatggtta gtaaaacatc gttatacaat gacctgagaa
      721 ttatcaataa aattattggc agtggagaag ttaagttgca gtcatctgca gaaggaatta
      781 gttttactgg taatgaacaa gcaattcaac gagcaatcaa gcaacttgta ttctattatg
      841 cgaatgacat ggaatcaaaa acattttttc aagttttaaa tatgttgttt gattcaaaag
      901 ttattagtgt cattcatgat ttgctattca atgattacag tgaactgact gaaaaggttt
      961 ctgattacta tgttcggtca ttgatttcta ctttagttat ccaatgtaac cgtttactga
     1021 aaggtcatca tttaactgtt gaagaagaat tcctcttcaa tagcattcgc tatatggaaa
     1081 cgtacattgt ggcaaatggt ttaattgaag aattgaaggc aaaattgaat cttccatttt
     1141 atgacgcaga taaagaatat ctatgtcgtc aactatttgc acatcgtgtg acaaataatt
     1201 taaaaaccag caatgaagag tacgctatga gcgttcataa attgattgaa agaatgagtg
     1261 agattgaaaa aattgatttg actcaaaatg atcgattata tcaatcctta ttgtatcatg
     1321 tgccagcaat ggtccttcgt ttaaaaaaag gtattaaaat tcaaaatccg ttattagaaa
     1381 atatcaaatc tcaatattca gaactcttta gcatagtatg gtacgccatg tccatccttg
     1441 aagaagaatt tgatgtgact ttaacggacg atgaagtttc cttaattttg attcattttc
     1501 agattgctat tgaagatcag tccaaagcaa ataatatcat cattatttgc caatacggta
     1561 tttcttctgc gcaattaatt tacaataaag ttcgacggtt tttaccagca aaagataatg
     1621 tagaaatatc aactttagat aagtttcatt tagctgaaat tggggaagtt gatttgatta
     1681 tttcctctct ttcctttgat gcaggtggta tacccttagt aagggtatct ccattggtaa
     1741 gtaaaacaga ttatatgaat attatggaag cttatactaa atatttaata gaagaacctt
     1801 cgcgatttat tcaaaatgaa ctgattccta aagcggttaa agttcccacc atatctaaat
     1861 ttgttcaatc atctttagta aaaacaggag tacaggtgga aagcaaagaa gaatgtttgg
     1921 atttgatgat ttccgaacta gaagccaatt cattagttac tgaagagttt cgtgcttcta
     1981 tcttctctag agagaaaatg gggaatacta atttagaaag tggtgttgct ttaccgcatg
     2041 cgaatccatc aatggtcatt gaatccaaca tttttattgt agcattaaaa aatgcgatta
     2101 attggggcga tcgaaaagtc aaattagtca ttatggttag tttagctgaa gaagatgtat
     2161 ctgagattag aggagtatat gaagaacttt accaatttat tgggcaaaaa gaaatggtcg
     2221 atcgcttgtt acaagttcat gatagcgaaa aattactaag aatttttcaa caaaaaggag
     2281 aataaaaatg tatctagata aagaattatg tttatttcaa ttagaagtga aagatcaaaa
     2341 agaattgttt caagtaatgt ctgaacaatt aaaaaacgct ggttgcgtga aagatagttt
     2401 cttagaaggt atcaccaacc gtgaacaaga atttccaact ggattagaag taaatcaaat
     2461 tgggtttgct atcccacata cagattccgc acatgtaaat agctctcaaa tctgttttgc
     2521 ctcattaaag gaaccactgg tattttcaga tatgacagat aaatctcatg aaatacctgt
     2581 acgtttagtc tttatgttgg caatgtcaca acctcatgaa caaattgata cactacaaaa
     2641 cttagttagc ctatttcaaa atgaagaaaa agtaaatgaa ttacttgcat gtaccacgaa
     2701 agaagcattt attgagttag tacattctgc aggagtttat taggaggaat agagagatgg
     2761 atattgtatt aaatgccttt gaatggttta ttggattagg gtcaaacgtc tttttaccac
     2821 tgattatttt tgtaatcggg atattatttg gattaaaacc tggtaaagca tttgtttccg
     2881 gcgttactgt tggtatcggt tctattggtt taggattagt tttaagttta ttatcagatg
     2941 gtttaggaac agcaattcaa caaatggggg aacaatacgg tggtggcctg aatatcctag
     3001 atattggcgt aggtgttggt ggacctttgg cttactctac atcattggga attttaatga
     3061 ttccaatttc attaattctt aacttcatat ttgtggtttt gggttggaca aaaacattga
     3121 atgttgatat ttggaacttt tggttcgcaa ttttcctagg tatgattact caatctgtta
     3181 cgggaaactt ttggtttggc ttaattggcg cgattgtcgc agtaatttta caatggttct
     3241 tagcagatcg tacacaaaaa actgttagcg aatttttcgg ctatccagga atctctatca
     3301 cacatatgat gggattatca ggagtactat ttgccaaacc agtaaattgg gtttttgatc
     3361 gtattcctgg atttaataaa atagatgcag atgcagaaac attaacaaaa cgtttcggtg
     3421 ttttaggtga cacagttgtt attggcctaa ttatcggtat tgtagtgggt attatggcag
     3481 gctatgacgt agcaggaatt ggaaacttag gaattatgac tgcggcagtg atgaagatta
     3541 tgcctaaaat ggtagcaatg tttatggaag gtttaatgcc aattgcagaa gcggcgaaag
     3601 aatttacgaa taaaaagtta aatggtcgcc aagtaaacat tggaatggat gcagcgttaa
     3661 ctgtgggtca tccaactatt atgtcaactt ctctattgct tgtaccaatc tcattattgc
     3721 tagcactaat tcttccagga aataaagtat taccttttgg agatttagca ttctttgctt
     3781 tcgccatctg tttaatgatt ccttatttta aaggaaatat tgtgagatca ttagtaggat
     3841 gttcgttgta tgttgttaca acattatata tggcaacatg gttggcacca attatcactg
     3901 atgtgtttac attagcaagt ttcgatgtgg ggacagcagg attagtaact atgttgttat
     3961 gtggattatg gccagtaggg ttgtttgttt tattagcatc ttactttggt gtaattggaa
     4021 taggtggatt aggagttgtt ttagtaggtg cttctatcta catgaacaaa gtgaagaaag
     4081 cttaatcata aaattaaaat aagaaagaag agatcaatat gaaaaaatta ttagttattt
     4141 gtggagcagg tcatgctact tctacaattg cagtagcaaa attatcaaaa tggttggaag
     4201 aagaaggatt aacagatcaa gtgaaaattt atcaatctaa aattgctgat gagctaaata
     4261 aaattgatga atatgatgct gtagtaagta caacaattgt ccctgatgta attaaagcta
     4321 aagtaacgaa tggcttgggc ttattaacag gtattggtac agatgccatt tatgcagatg
     4381 taaaagaaag attaggtgtt taatatgcgt tttcatcatg acactttgac tattgaaaca
     4441 gtttcagggc gtccttcgta tcatgatatt cgaaaagaac ttttagatac agttgataca
     4501 gcaggtattg agaatggaat gttagtaatt tcgagcccgc atacaacttg ctcactcttt
     4561 tttgaagaaa cgatgcatga cacaaacttt tttggtgatg attatttaca tgtagacatc
     4621 aacaatgtaa tggaaacgat tattccaaaa atgacgagtg aaaatcaata caacagtccg
     4681 gggccaaaac acattgattt tggaacaaga ttatctgatc ccaattaccc tgcagaaaaa
     4741 tgggtcatgc taaataccga tgcacattta cgttcatcta tttatggaac caactcaatg
     4801 actttaattg ttcaagaagg taaactttta ttaggagaaa tgggccgtgt ctattttgtc
     4861 gattgggatc aactaagaga gcgaactaga acaatcaatc tcatgattat gggagaataa
     4921 acgggggaat agagaatgac aattgctcca tcattgttaa atgcacctat cgcagaagtt
     4981 aaagagttat ttgaaatctt aaaagaaaag aatgttgatt atttgcatat tgatgtaatg
     5041 gatggctgtt ttgtaccaga tatggctttt ggaccaagtg ttattaaaga tattcgtcca
     5101 ctaacagacg ttgtgtttga tgttcatttg atgattgata caccagaaaa aattgtcaca
     5161 agatttgtag aagcaggagc agatattgtt accttccaca ttgaagctac caaacacgca
     5221 atgaaggtca ttcagttagt caaggaagca ggagcaaaag tagggattac ttttaatcct
     5281 gcaacaccag tcagttttat tgaaccagta ttatctttag tcgatcaagt attagtaatg
     5341 acaatcaatc caggaacaga gaataagcat tttattcaag agacagtagt taaaatcgaa
     5401 cagttagatg taatacgcaa acaaaatgat tatacgtatg atattgaagt agatggcaag
     5461 attgataatc aaacgattaa agtttgttct aaagcagggg cggatatttt tgtatcaggc
     5521 ggtttcatct tcaatgggaa aaataccccg caagaacaaa ttgataaact aaatcaatca
     5581 ttagaggaag tgaagtaaaa gatcatgacc aataaagacc aaaataaaaa ctatttagaa
     5641 gcgattatct tagaattaca aaataactca aaacgtatta attctgtaga gttagaacaa
     5701 tttagtgaga aaattgttca agctaaacac atctttctag ctggtgcagg acgctctgga
     5761 attgccatgc aagcatttac gaatcgctta atgcatttag gtttttcagt cagccttgtc
     5821 ggtgaaatta gttcaccaca ttcgcaacct ggagatttat taattatctg ttctggctca
     5881 ggtgaaacag gtagtttaaa aaatttagcg gccaaagcaa atcaaagtgg tgtaagcatt
     5941 gctttagtta ccatgaaaaa agactcgtcg attggtcaat tagcagattc tgttcttgta
     6001 ttaccaggta ttaccaaaga tgaaaatcaa cgagaagaag atactttttc acaacctatg
     6061 ggttcaagtt ttgaacaatt agcatttctc acctttgata gtttagtgtt atctttaatg
     6121 gataaaacag gtgaaagctc agaaacgatg tttgctcgac atgcagattt tgagtagtag
     6181 attttaaaga taaaaaacca ttctctaaat gctaattatt cagcaaatat gagaatggtt
     6241 tttttatttg attagacata acttaataat tcagtagttt tgagcgtaag cgtgtcatag
     6301 aatggtacct aaacacaaat agcccgattt gatacactca aatcaggtta tttgtggctt
     6361 atattattta cagatttctt gtatctgctg attccatggc aaataagcgc gtagcgcttc
     6421 aggtgtcatc gaagacagat tgggaatctc tgtaaaaaga tgatttaaat atttgcggat
     6481 gtttaaccca ttcgcaacag ctgtctggat aatactcaat ataattccat ttgatctagc
     6541 gccttttaag cttttagaaa atagccagtt ttttcgaccg atcactaact ctttgatggc
     6601 acgctctgct cggttatttg aaagttccaa gcgtccatca agcaacacaa tctgaagtcc
     6661 gtctttatga ttgactgcgt attgaaccgc ctttccaagc tgcgattttg gcaaataagc
     6721 aaaactctca caccaatgaa aaaattcagt catcacaggc tgcaacgttt ccttcctcgc
     6781 tacaaacctt tcttctgcgc taagcttatt aaaggtccgt tcgattcgaa acatcttgtc
     6841 acagaagtct aatcctattt tggcatgact ttttggggac ggttgtttac ccaatgcttc
     6901 aaaaaacttt ctgcgaacat gcgcccaaca ataaacattg tttaccggca gctttccata
     6961 ggccgcgtaa ccatcagtat gcaagaaacc gctaaatgat tctaaaaaac tttttggctc
     7021 atcaacggaa cgcgaatttg cgtgctcgta gagcacaact tgtttttctt cgcttttacc
     7081 tgaagcatat aaccagtaaa aggtcttttc tttatgagta ttcaataccc gatagcttgt
     7141 ctcatccgcg tgtaagacct cctgattgct taattcttga tgaaggcaat tgtagattgg
     7201 ctctaaaacc atttcactta ccttgatatg ccaattagtg atttttttac gatcaagagg
     7261 gaaaccaagt gcatgccatt ctttttcttg tcgatatgcc ggcacctttt taacaaattt
     7321 ttcccaaata ctctgtgtca caattgaaga ggaacctaga ctattattga tcaacggttt
     7381 aggaacaggc gcttttttga tttttgtttc tcctaatgct tcacattctc tgcaatgata
     7441 ggcatgttgg tagatgactt gtttttctat atgggcagga acatacaatg gctcttctct
     7501 aagccttgtc gcaccgatat catgtaaagt atgaccacag tcttcacaca aacaatcggc
     7561 accttttaat tcataatgaa taggaacttc aggcagacct tctaatgaaa gtgctttgag
     7621 tcccttgcgt tttctgcgtt taaaagaaga taccgtaatg ggttcctggc tttcttgtcc
     7681 agtgtgctct ggttcattaa aagttgagtc gtctaataaa tcgatttgcc catctacggg
     7741 tggtgtttct ttttttcgtc caaacaagcg tcgattcatt tcagcaattt gttctctaag
     7801 aagagtattt tcctgttgct ggatttcaag caactgtgtt aattgcttga tttgtacaag
     7861 aagttcagat tccatttttg tcatttttac accaccattc tagcgatttt cttacatcta
     7921 ttataccatt ttatgacaac gaaaaacaga caaaatcctt gatttaacaa ggttttcgtc
     7981 taatatatcg cacctttttt cgtttctttt agcgtacatt tttcttcgat tgaaaggcca
     8041 gtcagtaaga cttcttttct ggaacgcggc cattgaaggt acccactttc aaatcttttg
     8101 tacattaata caaatccatt ttgatcctag taaagagcct tgaagcgatc tttcttcgta
     8161 ccgcaaaata agaaaagcgc ttgatcgtaa acatcaaggt catattcttc tataacgaaa
     8221 gctgctagtc catcaattcc ttttcgaaga tctgtttttc cacaaacgat aaaaatatgt
     8281 ttcaatgcag ttgcatcaat gagcattggc attcaactct tttaacaagg tatacaaaag
     8341 atatttatcc attccattat aaataaatat ttccttatct tccgatttga ttctgcaagc
     8401 tagttttggc ttggtgtcaa tttgcgattt ttgagccgca cttgaaaatt gaactggtac
     8461 aatatctttt ttattcattc gtttttctcc ttctatattt gatagtttaa ttatagaaga
     8521 aagataatcg gcgtacgaga tactatccta tgacacgctt acatttaatt taatattttg
     8581 cctcaaatta gccacacttt ttatagaact aagaggaaaa atgaagtttt cttcctatta
     8641 atatagttgt ttgaaatctt aaatatagcg ttttacgatg taatgggaag tatggtgaat
     8701 aaatcaacta tgttcgtacg aagcatttac tcaactaatt taatcgaatc tttcaataaa
     8761 caaatcaaga aatacagcca ccgcaaggaa cagttccaaa atgaagagtc gatggaacgt
     8821 ttcttagtct cgtcttttga tacttacaac caaaaattcc taggtcgcag tcataaaggt
     8881 ttccaacagg ccgaaggcga gctcgaacaa atgctgagtc aaccgatgga gaattagaaa
     8941 caatctacag tagggaagaa ccattgacac aaaattattg acgctcccaa ctaaaagtgt
     9001 caaaataatg atgaaatcat ttagttctta taaattagaa aagagaaaat aaatattcca
     9061 tatctacttt attggatccg taacatgact aaattctgaa tactttttaa agcttatcat
     9121 ttggtaaaac tgaatataag ttaattatat agactgaaaa ttttgaattt tcagaataga
     9181 ataacttaaa agtactgaca aaatttttta aaggggcaat attaatgttc tgagataatc
     9241 aaatcctacc gaaaatatca aaaatccaat gtgacacaat cagattgtgt ctcattcgag
     9301 ggaatcacac aatcccacgt ggctctaggc agaattgaat ttttgcttgg ggctttttcc
     9361 ttatattgat tttgaatgag acttattata tgttataatg caattaagta tcattggaga
     9421 gggcgaagaa aatgaagacg gtagaaccta ttcgagacaa aaaaaagatc gatgcgatga
     9481 aggcaatctt ggcttcggga aaatatggcc aacgaaattt ggttcttttt tcgattggga
     9541 tcaacaccgc ctatcgcatt tctgatttgc gtcaattgaa gctggccgac gtgctggaaa
     9601 tttcacgcgg tcgcgtggtg gtcaaagatc gattagcgat gaaggaacaa aaaacagcta
     9661 aacataattc ggtctatatt tccaacaaat tgcgaaaaac gattttggag tacgttcaac
     9721 aggaatttcc tgatcaactc cttcagcaag attttgacgg ttatttgttc cctagtcgaa
     9781 aagggacgaa tgaaccgctc aaccgccaaa gtctgtggcg aatcattcat gatgctggca
     9841 cggcaatcgg attgaaagac attggcccac attccatgcg aaaaacgttc ggttactttc
     9901 tctataaaca ggggaccaaa acagaaatca ttcagtcctt gttgaaccat tcttctcagc
     9961 gcgaaacatt gcggtatatt ggtattacac aagaagacaa agatacagct gtaaaaagct
    10021 tagatttata atataaaagt taagtttaaa catggatagt tttaaattag tagattgagt
    10081 gttgggagga aagaatgagt acattatata ttataggaaa cggttttgat ttaagtcatg
    10141 gtttaaaaac tagatacact gatttttcaa aatgggttga aaaaagtcat agtgaaatat
    10201 tcgataaaat agacacaatg ataaatgaaa ataatgaggt gagaagtaca gctgatgata
    10261 agataggttg gtttaatttt gaaagtagtt tgcatgagtt aattcaaagt ccagtctttg
    10321 aagattatgt agaagagtct aaggattatc tcataggtta tggacatgaa gattggtcag
    10381 attctgccca tcatgatttt caaaatatgg tttatgatta tgttgtttct ctagaagaat
    10441 tacaacaact tttcaaggaa tggatacgca aagaaataca gccgacaata gaacataaaa
    10501 ttccaaaata ccaattttcg tccgatgtac tattctttac attcaattat acagatgttt
    10561 tggaaattct atacaagcaa ccgaaagaaa acatatatca tattcatggc agttatgacg
    10621 atataataat cggccactcc gagaattttg acaactttat aggagat
//
LOCUS       DAZKKW010000087        10475 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.089, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000087 DAZKKW010000000
VERSION     DAZKKW010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10475
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.089"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(339..4736)
                     /locus_tag="ACG8PO_002237"
     CDS             complement(339..4736)
                     /locus_tag="ACG8PO_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019788126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Eco57I restriction-modification methylase
                     domain-containing protein"
                     /protein_id="HFQ0207531.1"
                     /translation="MTTALKSKAKYFDYLPKYGSKLEHFLGIPNLVDAINTYRVTVNQ
                     IPNEVAIGTRRYSEVAARKIASNVGIDQQSMKYVDVLRELRVRNNITPTIADLFHKLR
                     KYGNVAAHENGIIPVSEAVESLVTLDTLLRVFARNLSGDKSLNPKEEITDEMFVITYN
                     TFDRKLIYIQSAKDADGTWKMYQGLEKIGDASVPENYEADFTPNSEYLREYASRRIKQ
                     YMTTAGVPFVLHWAQLAVNKKNQFFRDHDVHEVLKRSGYNPQTIGEDDTGKPNEWFSV
                     TVDVAKKAIQAVKDGKGSLDSEKDDFDEFKINFRPEQKDAIEQTKTVFKTKDEMLWNA
                     KMRFGKTLSSLQVIKESQYKKVLIMTHRPVVSDGWFTDFKKIFTDGSYIYGSKQDGEK
                     IQNLVDTEKPFIYFASIQDLRGSEWAGGKAGDKNYEFLAIDWDFIIIDEAHEGNETEL
                     ANNVKAKLRKEHTKVLELSGTPFNLFDKYDEDNIFTWDYTMEQEAKEKWSVEHPNEPN
                     PYEGLPKVSMFTFEIPNKFDYFDENKAFNFREFFRVKEDDETKLLHEGDVRKFLDYIT
                     TNDEKTNFPFSKPEFRENLRHTLWLMPGVKEANAFERVLETHPIFKDYNIANVVKTGD
                     SEHASDSDLELVRKAIGNPAKSKTITLTVRKLTTGVNVPEWTGVFFLSNTESPTSYLQ
                     AAFRAQTPFNHAELGIKKNCYIFDFAPDRALKIMSGSVGLTSKKGKVNSSEQKKKLER
                     MLNFLPILGKQGNVMKEFSVDRMLTALKKAYAEKAVRTGFEDSSLYNDNLLNLDQADL
                     TKFEDLKKIVGSSKPTKVKDFIVSDNGMSEEEYEKASRAEKKPKRQRTKEEQEAIDRA
                     KRLREQRNKMISILRGVSIRIPMMIYGMDIDIKDDVTIEMFLNLVDEASWNEFMPEGL
                     SKGRFREFIEYYDADVFVEAGRIIRRRAKSYDELDVIERTRKIGELFSTFKNPDKETV
                     LTPWRVVNMHLGNTIGGLSFFDEEYQNSTENGQPIVRWRKTDCTDDIFKENRKILEIN
                     SKTGLYPLYAATSMYYVQLLKQSDINAGRVDSEAIWEKVLSEQIFVISKTPMAKTITK
                     RTLIGYNAWKVNVEYSSELVTSMKRSINDGLTNLKGVYKNVKFDVIIGNPPYQEKDGG
                     SGASATPLYNLFVETSVKLSERYVSMITPSKWFAGGKGLDKYRDFMLNSSSLKVMVDI
                     VNGKEIFPNTSIGGGVSYFLWDKKYNGLTQYTNQHDGVSNTQERKMNEFPVFVRYNEA
                     ISAIHKIGEHASVGDFVISRNPFGLPTKIRGEEVQDSRNSIKVISSGGVGFVPLEDIT
                     KGQDMVGKYKVISSSLTSEHAGEPNKNGMFKVISTNRVIKPNEVVTDSYLVLFSADTE
                     TEAINYLKYLRTRFYRFLLLQAVTSIHLSKDKFMFIPVQDFTSGSDINWNVGIEEVDM
                     QLFKKYSFDASEILFIDQTIVPILHEEYY"
     gene            complement(4714..5499)
                     /locus_tag="ACG8PO_002238"
     CDS             complement(4714..5499)
                     /locus_tag="ACG8PO_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006701865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylase"
                     /protein_id="HFQ0207532.1"
                     /translation="MVEDMVMVEEEQGRLIKSKKRVQKHGEVFTPQRTVKDMLDLPGI
                     KEACEELQTTFLEPSAGEGAFLVEVLTRKMAMVAREYNESLEQYENYSLLALSTLYGV
                     ELLEDNAQTCVMNMFQVFNDLYREQSKKHGTAMKRKVQDSAKTLISRNIKQGDFLTKR
                     TPDDNPIIFSEWTPKTLRKGQKKITVNRTEYSLEDIYSGVTREFGEAYSALPVFEQLD
                     LFSFDDEVDEEVSNDPILRYVAMPITEVYKEEMEEIDDDSVEI"
     gene            complement(7255..8031)
                     /locus_tag="ACG8PO_002239"
     CDS             complement(7255..8031)
                     /locus_tag="ACG8PO_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0207533.1"
                     /translation="MMKKNYLKTGIFLVIGTILVIQLSGCRNKDKDFYGYWSGTAEKI
                     PVDYNFKITKDRVIELQKNGEMVDFKYTLRHKKFDSTTFRSIMISKDELVKAIPELKE
                     TTGISNITFMPIDGEKDKLTVLVNLDEHLSTATLYRTTKDESPIGKVEKKNDTISRQT
                     VDKKIDTFIKTYAAIPPTWKLVNEDKGITINTDTEIPTDAQSLSDLLANLTGVSNAVS
                     EQVGKDIPLRLLENGKKDYSVVFKNGKVTDYNEIFEKVIE"
     gene            complement(8232..8558)
                     /locus_tag="ACG8PO_002240"
     CDS             complement(8232..8558)
                     /locus_tag="ACG8PO_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0207534.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKATLDNGSAININVEYYPKKDRVYVEIPYT
                     EVLMLN"
     gene            complement(8604..8966)
                     /locus_tag="ACG8PO_002241"
     CDS             complement(8604..8966)
                     /locus_tag="ACG8PO_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HFQ0207535.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGADEVKKSTEQTFS
                     EMKEQIVHGQETGKLYLIIGWVSMVVSLAFIPVVFGAVAVIMGYLYRETDEKMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            complement(9003..9218)
                     /locus_tag="ACG8PO_002242"
     CDS             complement(9003..9218)
                     /locus_tag="ACG8PO_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0207536.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPINELFSDVFNKVNS"
     gene            9345..9680
                     /locus_tag="ACG8PO_002243"
     CDS             9345..9680
                     /locus_tag="ACG8PO_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0207537.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(9756..>10475)
                     /locus_tag="ACG8PO_002244"
     CDS             complement(9756..>10475)
                     /locus_tag="ACG8PO_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0207538.1"
                     /translation="LSLMTFIGLIKKLDDEDVPLKMLEYLNRLKEEKALGNFNHKRSN
                     VYRLDVFNATSVIQKLNEDILPLLEKIHFTYQYFSYEWVKICFNEKEAKRVFPQNSRS
                     ELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVCERLRNVGDNSVKNALIKNRG
                     YFVKQGFTLERASKDIKKLYNVKASSSFLIYLAKGAGIDETIKELILSGAGIVPLYQF
                     TSVTRDDGRRVRKRKDPIVLN"
BASE COUNT         3074 a         2222 c         1545 g         3634 t
ORIGIN      
        1 attacacact agtttaaact aaaacaaaca aggaaaaact taccatttaa tcataaaatt
       61 agctcatatg aatatattag aatgtaaaat agactggtca aaatctggtc aaaaaagagg
      121 actaatacag gtagtcctct tttttgttat tttgatattc taaatcattt tttattggat
      181 tattcccttg ttcgatgtag actcaaatca atctctttac ttgctaacat cctttatttt
      241 accttgacct gttgataata gacagttaaa tcttgattta aagtggggca attaatcaag
      301 aaaacagggt tctaagcctc atttgttagc ttgttctctt agtaatattc ctcgtgaaga
      361 atcgggacga ttgtttggtc aataaacaat atctcacttg catcgaaaga atatttcttg
      421 aacaactgca tatcaacttc ttcaatacct acattccagt taatgtctga acctgaggta
      481 aaatcttgta ctggtatgaa catgaactta tctttggata ggtgaataga tgtcactgct
      541 tgaagtaata aaaatctgta aaaacgtgtt cgtaaatact tcagataatt aattgcttcg
      601 gtttcggtat cagcagaaaa tagcactagg tatgaatctg taactacttc attaggttta
      661 ataactctgt tagtagaaat caccttaaac ataccatttt tgttcggttc acctgcatgt
      721 tcgctagtca gtgagctact aatgacttta tactttccaa ccatatcctg tcctttagtg
      781 atatcttcca gtggaacaaa gcctacccca ccactagaga ttacttttat tgaatttctg
      841 gaatcctgca cttcttcccc tcgaattttc gttggaagcc caaatgggtt acgtgatata
      901 acaaagtcac caacactagc gtgttcccca attttgtgaa ttgcagaaat ggcttcatta
      961 tatcgcacaa acactggaaa ctcgttcatt tttcgctctt gtgtatttga tacaccatca
     1021 tgttgattag tatactgtgt caatccatta tatttcttat cccacaagaa atagctaaca
     1081 ccgccaccaa tactggtgtt tgggaatatt tctttcccat ttactatatc caccatcacc
     1141 ttcaagctag agctgttaag cataaagtca cggtacttat ctaacccttt accacctgca
     1201 aaccacttcg aaggagtaat catcgaaaca tagcgttctg ataacttaac agaggtttct
     1261 acaaatagat tatatagcgg tgtagcacta gctcctgaac ctccatcttt ctcctgataa
     1321 ggtgggttac caattatcac atcaaatttc acgtttttat aaactccttt caaattcgtt
     1381 aaaccgtcat tgattgaacg tttcatactc gtaactaatt cacttgagta ttcaacattt
     1441 accttccatg cgttataacc aatcaaagtc ctcttcgtta tcgtcttagc cattggagtt
     1501 ttagaaatga caaaaatttg ctctgataat actttttccc atattgcctc tgaatctacc
     1561 cttccagcat taatatcgct ttgtttaagg agctgaacat agtacatact cgttgctgca
     1621 tataaaggat atagccctgt ttttgagttt atctcaagaa tctttctatt ttccttaaaa
     1681 atatcatctg tacagtcagt tttcctccat cgcacgattg gttgaccgtt ctccgtagaa
     1741 ttttggtatt cttcatcaaa gaaggaaagt ccaccgatag tgttaccaag gtgcatatta
     1801 acaactcgcc atggagtcaa tacggtttcc ttgtctggat ttttaaaagt gctaaataat
     1861 tcacctattt tcctagtacg ttcaataaca tctaactcat cataagactt tgcacgtcta
     1921 cggattatgc gtcctgcctc cacaaaaacg tctgcatcat agtactcaat aaactctctg
     1981 aatcgccctt tacttaaccc ctctggcatg aactcattcc atgaagcctc atcaaccaaa
     2041 ttaagaaaca tctcaatagt aacgtcatct ttaatatcaa tgtccatccc ataaatcatc
     2101 ataggtatgc gaatagaaac gcccctcaaa atggaaatca ttttatttcg ctgctctcga
     2161 agcctctttg ccctatctat tgcttcctgt tcctctttgg tacgttgacg ttttggtttc
     2221 ttttctgccc tcgaagcttt ttcatactcc tcttcactca taccgttatc tgaaacaatg
     2281 aagtctttca ctttggttgg tttacttgag ccaacaattt ttttaaggtc ttcaaatttg
     2341 gtaagatcag cttggtctaa atttaatagg ttgtcattgt aaagagagct gtcttcaaag
     2401 ccagtccgta cagctttttc tgcataagcc ttcttcagtg ccgtaagcat tcggtcaacg
     2461 ctaaattctt tcatcacatt cccctgttta ccaaggatag gtaagaaatt tagcatgcgc
     2521 tctagttttt tcttttgctc tgaactatta acctttccct tcttgctagt tagtccgaca
     2581 cttccactca taatttttaa agcacggtca ggtgcaaaat caaagatgta gcagtttttc
     2641 ttaattccaa gctctgcatg gttaaaaggt gtctgtgcac ggaacgctgc ctgcagataa
     2701 gatgtaggac tttctgtgtt tgaaaggaaa aatacccctg tccactctgg aacattaact
     2761 cctgttgtaa gcttgcgaac tgttaaggtt atcgtcttag acttagctgg attaccaata
     2821 gctttacgca ccaattctaa atctgaatca ctagcatgct cactgtcacc tgtcttgact
     2881 acgtttgcaa tattgtaatc tttgaatata gggtgcgttt cgagcactct ttcaaaagca
     2941 tttgcctctt taacccccgg catcagccat aaagtatgcc gtaagttttc tctaaactct
     3001 ggttttgaga aaggaaaatt agtcttttca tcgtttgtag taatataatc aagaaacttg
     3061 cgtacgtcac cttcatgaag caactttgtt tcatcatctt ccttaaccct aaagaattct
     3121 cggaagttaa atgccttatt ttcatcgaaa taatcaaatt tatttggtat ttcaaaggtg
     3181 aacatagata ctttcggtaa accttcgtat gggttaggtt cattgggatg ctctactgac
     3241 catttctcct tagcttcttg ctccatagta taatcccaag tgaaaatatt atcctcatca
     3301 tacttatcga aaaggttaaa tggtgtaccc gaaagctcta agaccttggt atgctctttc
     3361 cgcaacttag cctttacgtt attagctaat tccgtttcgt ttccttcgtg agcttcatca
     3421 ataataataa aatcccagtc aatcgctaag aattcatagt ttttatcccc tgcttttcca
     3481 cctgcccact cagaaccgcg taagtcctga atacttgcaa aatagatgaa aggtttttcg
     3541 gtatcaacaa ggttttgaat cttttctcca tcttgttttg aaccatagat gtacgaacca
     3601 tctgtgaaga tttttttgaa atccgtaaac caaccatcac ttacaacagg tcgatgagtc
     3661 atgataagaa cttttttgta ttggctctct ttaatcactt gaagagaaga aagcgtctta
     3721 ccaaaacgca tcttggcatt ccaaagcatc tcatctttcg ttttgaatac agtcttagtc
     3781 tgctcaatag catctttttg ttctggtcta aaattaattt tgaactcgtc aaagtcatct
     3841 ttttctgagt caagactacc tttaccatct ttaaccgctt ggattgcttt cttggcaacg
     3901 tcaactgtta cactgaacca ctcattaggc ttccctgtgt catcttcgcc aatcgtttga
     3961 ggattatagc ccgaacgctt taatacttca tgcacgtcat ggtcacggaa aaattggttc
     4021 ttcttattaa cagccaattg cgcccaatga aggacaaaag gtacccccgc tgttgtcatg
     4081 tactgcttta ttcttctact tgcatactct cttaaatact cgctattagg agtaaagtct
     4141 gcctcataat tctctggtac tgatgcatcc ccaatctttt ccagtccttg gtacattttc
     4201 catgtgccgt cagcatcctt ggcactctgg atgtaaataa gtttacggtc aaaggtgtta
     4261 taggtgataa caaacatctc atccgtaatt tcttcctttg ggtttaacga tttatcccca
     4321 gacagattgc gtgcaaagac tcttagcaag gtatcaagag taactaaaga ttcgaccgcc
     4381 tctgaaacag gtataattcc attttcatgt gctgcaacat ttccatattt tctcaactta
     4441 tgaaataagt cagctatcgt aggagtaata ttatttctga ccctaagctc gcgaagtaca
     4501 tcaacatact tcatactttg ctggtcaata cccacatttg aagcaatctt ccgtgctgct
     4561 acctccgaat aacgtctagt tccaatagca acttcattag gtatttgatt aacggttaca
     4621 cggtacgtgt taattgcatc tactaaatta ggaatcccaa ggaagtgttc tagcttagaa
     4681 ccatatttag gtaagtaatc gaagtattta gctttagatt tcaacgctgt cgtcatcaat
     4741 ttcctccatc tcttccttat aaacctcagt tatcggcatc gctacataac gtaatattgg
     4801 atcatttgaa acttcttcat cgacctcgtc atcaaatgaa aacaaatcga gctgttcaaa
     4861 tacaggcaga gctgagtatg cctctccaaa ctctcgtgtt acccctgaat aaatatcctc
     4921 caaagaatat tcagttcgat ttacagtgat ttttttctgt ccttttctta gcgtcttagg
     4981 tgtccactca ctaaaaataa tcggattatc atctggtgtt cgttttgtaa gaaaatctcc
     5041 ctgtttgata ttcctagaaa tcaaagtctt tgcactgtct tggactttcc ttttcattgc
     5101 ggtaccatgt tttttcgatt gttctctgta caaatcgttg aatacttgaa acatgttcat
     5161 tacacaggtc tgagcattgt cttcgagtaa ctccacgcca tatagagttg aaagcgccag
     5221 taaggagtaa ttttcatact gttctaggga ctcattatat tcacgagcaa ccattgccat
     5281 ttttctagtc aaaacctcaa ctaagaaagc accttctcca gctgaaggct ctaagaaagt
     5341 cgtctgcagc tcctcgcaag cctcttttat gcctggcaaa tccaacatat ctttgacagt
     5401 tctttgcggt gtaaatacct caccgtgctt ctggacacgt tttttacttt tgattagtcg
     5461 tccttgttcc tcttcaacca tcaccatatc ttcaaccact aaaaatcacc catcttccca
     5521 acacaaataa ccgaaacgga tgctacgaat cggttatcac tttcatatat gggcttttgg
     5581 gcagactgct ggctagtact gtgattgcgt gtcatcatgc ccttttcctc cttacaagtc
     5641 aagattttta cttgaaaaaa ctcgacaatt tctaattatg cttatgttca attttaacac
     5701 atttttgttt tttcctctat ctcaaaataa agaacaaaat atagcttaac tggagaatta
     5761 tagagtttgc ctcttgcaac acaggggagt tagcgaagat gttcgtctgt cctattttat
     5821 ttttatgaac atgaaaaaag accaccaaac taggcagtct ttgacttatt attatttact
     5881 tttagctatt cccattaaat tttaccactc gtcccttttt cgccctcttt agatttagcc
     5941 gagggtcgtg tggtaaaagc tcattattgt cttaacgtgg tcaaaaacct ctttcctacg
     6001 tttatttgat aacccatact gacgaaacac ctctttcatt atcgcaatca tttgctcatt
     6061 agataacttt tcatcatacc catcaatgaa gttctctaat gactgactat ctaaagcttt
     6121 ctttatttca aagaacaagt cataaacagg tttatcttgc ttcttcagcc agtgaacata
     6181 gccctccctg tgctcttttt cccgttcacc tgttactcct tgctgtttag gtaatcacag
     6241 aatgttgacc gactgacttc tatacctgta tcttcttata ttctcgataa agacttctta
     6301 atggtgagtc cttcttgata atacttcagc ccagcctgta aaacttcgtc tggaaacctc
     6361 tttcgatgtt ctgtcttgtt cgctgaaaaa ctcttgtact catcatcaaa gctgagtgta
     6421 aataccacaa caagatgttc gtacgatagt ctgaccagaa aactgacttc cttgtcgttt
     6481 gtacccaaat tttcatatga agtagataaa agaactgtat acctggaaaa gacgtcttct
     6541 tttacgaagt caatgcacaa tagacgtaga ataggctatc ttcttcgaca gttagtgaac
     6601 ctatatcgtc tgctaatagg atatccgact ttaaatctaa atatttttcc tctgattttt
     6661 taatttccct cttgaaataa tctattgtaa tttgtttatc tgaattgttt gcaattaacc
     6721 cctgcagaac ttctctagct tgttcaacat cccgtattac catggaaatc actaggtatc
     6781 aaactgagtc atacgaagac aaaaacataa aaattgacct gttaaagggt cactgaataa
     6841 catataaaat caataaaacg gagtcagaca cggagtcaaa aaacgctgag agtaaaaaat
     6901 atacagtccc ataggtaaag tgccacagga ctgtatattt ttttatttca gattacctga
     6961 aatgattcca tttacttttt atctcatttt tccctactag ttagtatcaa atactctcct
     7021 attttctcaa taaactattt tgggttacat ggtatctgaa acagctaaaa ccttgtcatt
     7081 acagcaaaaa atcactttat ctccgtttca tttcagatac atttcactta agcggattcc
     7141 caaaaaataa aagaaaaaga aacccctagt tagcctatgc tttctaatga ttcctctcga
     7201 aacacggtca aaaaacaacg gggtagggta gttaataaaa agatagaaaa accattattc
     7261 aatgactttc tcaaatattt cgttgtaatc agttaccttt ccgttcttaa ataccacaga
     7321 ataatctttt tttccatttt caagtaacct aagtggaata tctttaccga cttgctctga
     7381 caccgcatta ctgactcctg ttagattagc taataaatct gataaagatt gagcatcagt
     7441 cggaatctct gtatcagtat ttatcgttat tcccttatct tcatttacaa gtttccaagt
     7501 aggtggtata gccgcgtatg tttttatgaa cgtatcaatt tttttatcta cagtttgtct
     7561 tgatattgta tcgtttttct tttctacctt accaattgga ctctcatctt tcgtagtcct
     7621 ataaagagtg gctgttgata agtgttcatc caaatttacc aacacagtta atttatcttt
     7681 ttcaccgtca ataggcatga acgttatatt gcttattccc gtggtttcct ttaattctgg
     7741 aatcgcttta actaattcat cttttgatat catgattgaa cgaaatgtag ttgaatcaaa
     7801 ttttttatgt cttagagtat acttaaaatc caccatttca ccattttttt gtagttctat
     7861 tactctgtct ttagttattt taaaattata gtctacagga attttctcag cagttcctga
     7921 ccaatatcca tagaaatctt tatctttatt tctacaacct gataattgaa taaccagtat
     7981 agttcctata accaaaaata tacctgtttt taaataattc tttttcatca taaagctcct
     8041 cttttaaagc atttaatcaa caatgtaaga acccaatttt taaagctctt taaaaacaat
     8101 ttccatcaat tattataaca ttatttttct catctatatt agtattttat ttacatggaa
     8161 tcaaaaaaac tccttagtaa gcttacacct ttaggagttc ctgtttggac ggtatacata
     8221 atacatttaa atcaatttaa cattaacacc tcagtatatg gtatctctac atatacacgg
     8281 tctttctttg gatagtactc gacatttata ttgatagcac ttccattgtc taacgtagct
     8341 ttagcccggt atgtgttatc tccattttca tgagtaatcg tcacatcatc acatgaccta
     8401 tttaagttgt actgttcttg taaaatctgt gtcaccaatg gtttagatga cttctctaat
     8461 ccagatgtac ctgtcatttt tgaaaaaatg atacctataa tagccaagac aacgatacct
     8521 ataatgacaa acttcttgtt catatttact ttattcaact ttttatctcc tatctccaaa
     8581 ataatttgct cttatccctc ttatcagtac attgacatcc ctaaaagtac accaaagatt
     8641 gctccagcta cccctgctat catcaaaatc gtacccattt tctcatctgt ttctcgatat
     8701 aagtagccca taataacagc aacagcacca aatacaacag gaataaatgc taacgatact
     8761 accatagaaa cccaaccaat aatcaaatac agcttccctg tctcttgtcc atgtacgatt
     8821 tgttccttca tttcactaaa tgtctgctca gtacttttct ttacttcgtc tgctccagtt
     8881 gtctcatctg tttcttgtaa tgctccgcaa aactggcaat acttactttc atctgttatt
     8941 tctttaccac attttctaca atacatgtct tctcctccta aaatcattca attgcttaat
     9001 agttaactgt ttactttatt aaacacatca gaaaacagct cgtttattgg tacacttaat
     9061 atctcagaaa gcattttcat ttgatagggt ttaggaatcg ttctccctgt ttgccacgaa
     9121 cttacacatt gctgtgtaac attcattttt ttggctaact ccgtttgaga aagtcctttt
     9181 tcttttctgt atcggctgag attacttact tttttcatga ttctcccctc ctgttttatt
     9241 atatacaaca atatgatgta tttcaactca taaaatataa aaaacttcaa ataccattac
     9301 aaatacaaca acttgttgta ctatactgag atgggggtgt attaatgaaa cagaaacaat
     9361 atgcgaaggt cattagagaa aagcgaaaag ctctcgggtg gacacaaaaa gaattagctg
     9421 aaaaaatctt ctctacacaa caggcagtag caagatggga aaattctgta acagagccta
     9481 acctcgatag cttaacagct ctttctagag ccctaggaac ccctgtaagc cattttctag
     9541 acaatgttgt cgtagattat gaggaagagt ttttggctct ctatcgttct cttagcactg
     9601 aagatgcagt tcgaacaatt gactatatga agctattaaa aaggcaagaa aatgaacgca
     9661 atcagctatt gaaagattga aaaataaata acccaccgaa ctttaacggt gggtgtattg
     9721 agatgaaata agattaagag ctatggataa cctgtctagt ttaatacaat tgggtctttt
     9781 ctcttcctta ctctccgtcc atcatctctt gttacacttg taaattgata cagaggaact
     9841 atcccagctc ctgataatat caattctttt attgtttcat ctatccctgc ccctttcgct
     9901 aaatatatta aaaaactact acttgctttc acattgtata gtttcttaat atccttagaa
     9961 gctcgttcaa gagtgaatcc ttgtttaaca aagtagcctc tgttttttat taaagcattt
    10021 ttcacgctgt tatccccaac attacgtagg cgttcacaaa cagtgttaat aagttctttc
    10081 tctgtcatta gatatgtccc ttgccacgat aagccctgta aaacttcatg tgcttcatct
    10141 aaaatcaatt gtttttcctt gctgagttct gatcgggaat tttgaggaaa tactctttta
    10201 gcctcttttt cgttaaaaca tatcttaacc cattcataag aaaaatattg atatgtaaaa
    10261 tgaatttttt ccagaagtgg aaggatatct tcattcaatt tttgaatcac agaagttgca
    10321 ttaaaaacgt ctaatcgata gacattgctt ctcttatgat taaagtttcc taacgctttc
    10381 tcttccttta acctatttag gtattcgagc atcttcaaag gaacatcttc atcatctaat
    10441 ttttttatca atcctataaa agtcataagt gatag
//
LOCUS       DAZKKW010000088        10094 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.090, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000088 DAZKKW010000000
VERSION     DAZKKW010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10094
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.090"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            131..3358
                     /gene="fss3"
                     /locus_tag="ACG8PO_002245"
     CDS             131..3358
                     /gene="fss3"
                     /locus_tag="ACG8PO_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HFQ0207539.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVAPVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYCQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            3471..3785
                     /locus_tag="ACG8PO_002246"
     CDS             3471..3785
                     /locus_tag="ACG8PO_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFQ0207540.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            3798..4172
                     /locus_tag="ACG8PO_002247"
     CDS             3798..4172
                     /locus_tag="ACG8PO_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFQ0207541.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            4182..4517
                     /locus_tag="ACG8PO_002248"
     CDS             4182..4517
                     /locus_tag="ACG8PO_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HFQ0207542.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            4600..5940
                     /locus_tag="ACG8PO_002249"
     CDS             4600..5940
                     /locus_tag="ACG8PO_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFQ0207543.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKICYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVEKNFFMKHIKGRGYVDTGGSVISEFYTPLVPKGYDFLDSISKLEKMND"
     gene            6018..6692
                     /locus_tag="ACG8PO_002250"
     CDS             6018..6692
                     /locus_tag="ACG8PO_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFQ0207544.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDVLLHIIIKDYTNNISEVHYATDNLELFLPLGYSRKDL
                     EVQLHD"
     gene            6685..6849
                     /locus_tag="ACG8PO_002251"
     CDS             6685..6849
                     /locus_tag="ACG8PO_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0207545.1"
                     /translation="MTEKEQVTKIVKKYNKSIADLSENATAKEFKTVIKYVADQANEK
                     QRKLVGLNKK"
     gene            6925..8067
                     /gene="dcm"
                     /locus_tag="ACG8PO_002252"
                     /pseudo
     CDS             6925..8067
                     /gene="dcm"
                     /locus_tag="ACG8PO_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
     gene            8057..8347
                     /locus_tag="ACG8PO_002253"
     CDS             8057..8347
                     /locus_tag="ACG8PO_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0207546.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            8606..9790
                     /gene="mobT"
                     /locus_tag="ACG8PO_002254"
     CDS             8606..9790
                     /gene="mobT"
                     /locus_tag="ACG8PO_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobT family relaxase"
                     /protein_id="HFQ0207547.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     II"
     gene            9787..9924
                     /locus_tag="ACG8PO_002255"
     CDS             9787..9924
                     /locus_tag="ACG8PO_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HFQ0207548.1"
                     /translation="MKRGIVMGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKG
                     K"
BASE COUNT         3343 a         1707 c         2281 g         2763 t
ORIGIN      
        1 aaatttttag gctcattcat tctcaaatcc attctattca gtcaataaaa attttaaaaa
       61 gactttataa acaaagatgt tcaagcgagc atcttatttt tttgtgctta atttgaaagg
      121 agaacatttc atgtataaaa aacaaaaaaa gaaattatgt gtattaacta ttttgagttt
      181 ggctgttcaa gcaattgttg gagcaattgc tcctacgatt acttttgctg atgaaattac
      241 ccatccacaa acggtaaccg tagaactaga tttagcacat caatatgctg ttgaaggaac
      301 ttttagtgat ggtcgtccaa tgtcagaagt cactgttccg cattatgcag tgtacaacgg
      361 ctttaaacaa gatattttct gtattgaacc aggagtcccg atttacaatg agttcactcc
      421 tggctatgag aaaaacccgt taccagatat gtccgataaa gccaaattag tttccgtttt
      481 atggaaaaaa gtaggtacag atgcagatac tcatattgtg gcacagaaga tgatctggca
      541 agaagttaat gggtataccc ttcactcaat aaaacgatca gatggtagtg ccgtaaatat
      601 cgcagccatt gaggcaaaaa taaatcaagc cattgacgat tatcagaaga aaccaacctt
      661 ccataatagt acggctaaaa cagtattagg gcaatcgact actgtgacgg atacgaataa
      721 tctgaatttg tcagagttcg atgaggttgt ggaaaatgcc gcaaacattg attatcgtgt
      781 gaatggtaat cagttgataa tcaccccaaa tgccaattca aaagaaagtg gtgtgctgac
      841 gcttaagaaa tctgctggta ccggaacacc tgtcgcctat aaaaaggctg gccaacagac
      901 tttaatggcc ggggcgattg ataaacctaa tacctacaca atcaaaattg atgtggaaac
      961 ggagggttct ttaaagatca aaaaagtcga taaagaatcg ggtgatgttg taccaggaac
     1021 ggttttccat ttagactttg gaaaaacttt acctgcaaaa gacgtgacta ctgacaaaga
     1081 aggcattgct acattggatg agattcctca tggtacaaaa gtaaccatta ccgaaaaatc
     1141 agtaccggcg ccttatacga ttgataccac ccccatgact accaccatta aagcaggcga
     1201 aaccatttac gtgacttcaa aaaacgcacg ggaaaaaggg caaatcattc tggataaaag
     1261 cggagttgaa acagggagtg atctttggaa tgacaattat tccttagctg gtaatacgtt
     1321 tgccattcgc aaagacagtc caaccggtga aatcgttcaa gaaatgacca cggatgaaaa
     1381 cggtcatgcg gaaacaccaa aagagattgc taatgcgttg gaattgggaa cctattatgt
     1441 gaccgaaact aaagctagtc atgggttcgt gaatacgttc aaacctgtaa aagtcgaatt
     1501 gaagtatgcc aatcaaaccg tagcccctgt taccagtaac gtaaaagggc aaaatcaaga
     1561 agtcactggc gaaaccactt tgacgaaaga agacaaagat actggggata aagcacaagg
     1621 taaggcagtc tttgagggtg ctgaatacac cttgtttact gctaaggatg gaaaagctgt
     1681 taaatggagt gaggctttta aaccagagtt agcgaaggga acgaaaggtt ctgatgaaac
     1741 ggtcactttg actttagacg aaaagaacca agttgccgtt aaacatttag ctatcaacga
     1801 gtattattgc caagaaacaa aagcgcccga aggatatagc cttgatgaaa caaagtatcc
     1861 tgtatccatc aaaaaggtgg atgatagcga aaaaaatgcc gtgattaccc gaaatgttac
     1921 ggcaaaagaa caagtgattc gctttggctt tgatttcttt aaatttgctg gatcagctgc
     1981 gggcactacc gaaactggct tcaatgatct gacctttaaa gtgtcgccat tggaagggac
     2041 caatgaaatc acaggtgccg aagatgaagc gaccacagct tataacgagc aattagggtt
     2101 tgatggttat ggcaagtttg aaaatcttcc ttatggggat tatttacttg aagaagtaga
     2161 agccccagaa ggttttcaaa agattacgcc attagaaatc cgttctactt ttaaggaaaa
     2221 caaagaagac tttgtgaaga gtgagtatgt ctttaccatt accgaacaag accaaaaaca
     2281 accaattaaa acggtgaccg ttccttacga gaagctgacg aacaaagcat tttctgttag
     2341 tttgaaccgt ttaatgctct atgatttgcc tgaggaagaa gatagtttaa cttctcttgc
     2401 gacttggaaa gacggaaaca aagaattaac ttctctggat tctaccgagc tagttgataa
     2461 attgagttac aatctacatg aaatcaaaga agactggtat gtggtagcgc aagccattga
     2521 tgtggatgcg acaaaagctg cccaagaaaa agatgaaaaa gccaaaccag tggtgattgc
     2581 agaaacaagc gcaacgttgg cgaacaaaga gaaaacagga acttggaaaa ttcagcataa
     2641 attaaccgcc gaacaagttt taaataaaac tattgtctta ttcaactatg tgtatgaaaa
     2701 taaggaagct tttgaagcag gcgataagcc agtagcgaag gatgttagct tgaataacca
     2761 agcccaaact gtcagttgta cagtggaaca ccatgtttct atccaaacaa aggcccacct
     2821 agaaaacggt tctcagacct ttactcatgg tgatgtggtg gatatgtttg atgatgtgtc
     2881 gattactcat gatgtactgg atggctcaaa agaagctttc gaaacaattc tgtatgcttt
     2941 attaccggat ggtacaaaca aagaaatttg gaaatctggc aagattgatt atgaagtaaa
     3001 tgataaagaa ttcaccaaaa ctgtgcttgc gaaaaaagta gataccggaa aatatccaga
     3061 aggaaccaag tttacttttg cggaaatcaa ttacgataaa gatgggaata tcaatggaaa
     3121 acacaatgaa gatttgaaag aaaaatctca aaccttaaca ccaaaagaag ttccaaccat
     3181 actaagtacg ccgaaacaac cggaaacacc aactgttcca agtggctctc aagaatctag
     3241 tcccacagtg aagaccttcc ctcaaaccgg ggagaaaaat tccaatgttt tactgttcat
     3301 tggttttact ttgatttttg cgacggcggg ctattatttc tggaatcgcc ggaactaagg
     3361 tgatgagatg atacaagtaa atcctaagaa ccaacgtgcg cgaccgcctc ctaaaaaagt
     3421 agtcgcgcta tttttgtatc caaaactaac aatgaaaaga ggaaaagaag atggaattga
     3481 aatttgtcgt gccagatatg gctgaaacct ttggaaagtt aagttatgcc ggtgaaggag
     3541 aaattataac agaaggatat ggacggaata ctacggtgat tggtcgcagt tatcatttgt
     3601 attccagtaa gcaacgggcc gatgatattg aggtggtagt agctgcggaa gccggtgaaa
     3661 aggattttga tcaagaccaa ccgctaaaag ccgtgaatcc ccatttggtt gccaaaggct
     3721 atgagattga gaatcgtggg tttactgatt acgtgttgta tgttgatgat ttagtgaaag
     3781 cataggagga aaagaagatg agattaacag aaggtattgt cgtagattca ggtttaacat
     3841 ttgggaagtt gcgtttttcc gcattacgcc gagaagtacg gaagcaaaat gaggatggaa
     3901 caattggtaa tgaggtaaaa gaacgaacct ataatttaaa atcctctgca caagggcgaa
     3961 tgattcaagt tagtatccca gctaatgtac ccttacgcga gtttgcttat gatgcagagg
     4021 tagagttggt caatccaatc gtggataccg ttgccaatta tgtttttcga gaaggaacta
     4081 ccgttaattg gttcattaag gcggatgatt tagtcttgaa gcggcagccg aatcaaggaa
     4141 atcctacgaa ccaaaatgaa agaaagaaat aggtgaagat tatggaaatt aatgttgaac
     4201 atattttaga ttgtctcgat caatatggaa aaggcgagct gactgaggaa caactcgtta
     4261 aagcattaac ctctgatgaa aaaatgtttc tgattataca tcaaggttta cttggagaaa
     4321 atagcgacag tgaagaagac tctgatgtac tagactggtt aggagaagaa gagtctttct
     4381 ttatagttgt cgaagtaaat gaaaacttat gtcggcaggc agaatccatt ttggaagaaa
     4441 ttggtgtgga gatgccggat gctattgaag gctttttaaa tcaattggtt gaaacgaaac
     4501 aactcccctc ctcgtagcag taaatgacta gcataacttt ttgaaattgc ctaatgaaat
     4561 cctattgggc tttttcgatt ggaggtggaa accatcaaaa tgtacaaagg acatcgcata
     4621 cgcgcaggag atcagcactt ggtttatcac tttgttcttg gctggctact cgctctgttc
     4681 atcggttgga tgagcgtctt ttattttcaa gagtttagac aatttgatat ttctaagcta
     4741 tcactttcta ctattgaaat cgtttggtct ataaaagatt tagtatgtct gcttggaagt
     4801 ctcgcttttt caggagctat gatattgctt tatattcact tttttcttga tcattggcga
     4861 agcttgtggc atcgacaaaa gctagctcgg atgattctag agaaccattg gtatgaagtg
     4921 aaacaaactc aaagtgaagg ctttttcaaa gatttgaata gtagccgaac cagagagact
     4981 atcagttact tccccaaaat ctgttatcga atgaaagatg gattactgtc tattcgcgtt
     5041 caaatttcac ttgggaaata ccaagatcag cttttaaaat tggaaaagaa gttagaaagc
     5101 ggactgtatt gtgagttagt agaaaaggaa ctcaaagact cctatgtgga gtatacttta
     5161 ctgtatgata tgatcgccaa tcgaattggg atagacgaag tagtggcaga aaatgggact
     5221 ctgcgattga tgaaaaatca agtatgggct tatgattctt taccccatat gttaatcgct
     5281 ggtggcacgg gtggagggaa gacctatttc ctcctcacca ttatcgaagc actattgaag
     5341 tcagatgcag aattgtttat cctcgatccc aaaaatgcag atttagctga tttaggtacg
     5401 gtgatgcctc atgtttattc acaaaaggaa gaaatttctg cttgtgtgga agatttctat
     5461 gaacgtatga tggctcgtag caaggcaatg aaagaaatgt ctaactacaa aacaggagag
     5521 aattatgcct atcttggact tccacccaac tttttaatct ttgatgaata cgtggcttat
     5581 atggagatgt taacgacgaa agaaagtgcg gtgattttga ataagctcaa acaaatcgtg
     5641 atgttgggtc gtcaatctgg tttctttctg attttggctt gccaaaggcc ggatgcgaaa
     5701 tatttaggag acggaatccg cgatcaattc aactttcgcg tggctttggg tcgtatgagt
     5761 gaactcggat attccatgat gtttggggag gtcgaaaaaa acttctttat gaagcacatc
     5821 aaaggtcgag gttatgtgga tacaggtggc agtgtgatta gtgagtttta tacgccactt
     5881 gtaccaaaag ggtatgattt cttagattca attagtaaat tagaaaagat gaatgattga
     5941 gtgatcttca atactgactt tatacttcaa gtaacgtata atacttttat atagtaggaa
     6001 gaaaggggat aattaatatg gaaagtaatg attcttattt agagtatgca aaagcaaata
     6061 aggataagtt tatttcttat attatcaaag ggaaatcccc cgaagttgga cgagacgcag
     6121 tttttatggc agggagtcct ggggctggaa aatcagaggt tgccattgga ttagctgaga
     6181 attatgataa ccatgtgatt attgatgcgg attactttcg cacccagttt cctgattata
     6241 atggaaaaaa ctctagtctc tttcaaaaag catcttcttg gttggtagag caggcattta
     6301 gatatgtctt agatcatggc tattctttta ttctggatgc aacttttgca atattaagtg
     6361 ctgaaaaaaa tatctcgcgg gcgttgaaaa aacattttcg agtgacgatt ttctatgttt
     6421 atcaagaccc taaaatcgct tggaaattta cacaagaacg tgaaattgca gagggacgtc
     6481 atgtgccgaa aaaggcattt attaacgctt ttttcaaatc aagagagaac attgcaaaag
     6541 taaagagtaa acatccagat gtattgcttc atatcataat aaaagattat acgaataata
     6601 tttccgaagt tcattatgct acggataatc tagaattatt tttaccacta gggtatagta
     6661 ggaaagattt ggaggttcaa ttacatgact gagaaagaac aagtaacgaa aattgtaaag
     6721 aaatacaata agagcattgc ggatttatcg gaaaatgcta ctgcaaaaga gttcaaaacg
     6781 gtgattaaat atgtagccga tcaggccaat gaaaaacaac ggaaattagt tggattaaat
     6841 aaaaaatagt aggacaaatt aataatgtat gagccaagag caaaatgttc ttggcttttt
     6901 tctttgcaat gaatgaggta atgtatgaga tttttagatt tattcgccgg tattggtggc
     6961 tttcgtttag gtatggaaca ggcgagccat cattgtatcg gtttttgtga aattgataaa
     7021 tttgctagac gtagttataa agcaattcat gatacaagta aggaggtgga aatgcatgac
     7081 atcacaagtg tatcagatga gtttattcaa tccctcggac cagtggacat cctttgcggc
     7141 ggatttccgt gccaagcttt ttcaattgcg ggaaagcggc aaagattttc cgatactcga
     7201 ggtactttat tctttgaaat cgctcggttc accactcttc tccgacctaa gcttttattc
     7261 cttgagaacg tccgaggatt gctcaatcac gaaggagggg ctacgttcga gacgatcctc
     7321 cgaacgctgg atggattggg gtatgacgtg gagtggtagg tgcttaactc aaaggcctac
     7381 attccccaaa gccgggagcg gatattcctt atcggacatt ctcgagacgc atgtaccgaa
     7441 caagtatttc ctatcattgg accgtcttca acatctgatc aaaacatcag aaatttggta
     7501 aatatcaatc cttccaatcg aggaatgggt ggacaagtct atggttcaga tggcgtagcg
     7561 ccaaccttaa caggtgatga aggaatcaaa atcgcactgc cagcaaggga tggaatctct
     7621 gtagcaggac tattaccggg aaagtttgaa caaggtaatc gagtttatga agttactgga
     7681 accgccccca ccttgtcaac gaaacaaggt ggtacaaaaa tcatgattcg aaaaaatgaa
     7741 acagaatatc aagaggtgaa gccagaagac agtgtgaatc tggcttttcc taattccaca
     7801 agtcgccgag gtcgtctggg aaaacagagt gttcatacct tactaacagg agaccaacaa
     7861 gcgatcgtca cggatcagta tcaaattcgg aagctaacac ctagagagtg ttggcgactt
     7921 cagggctttc ccgattgggc ttttgatcga gattctcaag tcaattcaga tagtcagttg
     7981 tataagcaag ccggaaactc tgtaacagtt ccggtcattt ttgatattgc cagaagatta
     8041 aaagagttgg atgtacatgg tttatgatga gttgattgga gaaatttatt gggtgattgg
     8101 aaagattcag tcagacccag aattagaaga acaattgcga cggttaaaat ttgatattcg
     8161 caagaatggt gtcaaagtcg ctggcgatcc atatttaatg aatgaggaga cggatgctcg
     8221 aattgaaata aatcaagtaa ttgcagaatt tgagcggatt gcggaccaag caaaagaacc
     8281 agatattcgt caatacttgt tagaaatgaa agcagagttg gaagtaaatg gtattacaga
     8341 tgagtaacct aaaccaacac aactgactgt taggcgctga ccgcggagga ttcgcgaagc
     8401 agaaatcctc cgtggtgctg tgcctgttca tggacggcgt tagccgacca tgatgtctca
     8461 cccccgtatc taacaggggg gtacaaaaac acaaaaaata agtaagcaac cattaagaat
     8521 gcttgttgta tcaaagatag taagtaattt gtataggtgt taactcattt gaaaaagtta
     8581 acaccttgaa tttggaggtg agaacttggc acaacggaat ttagattatc ggctattaaa
     8641 agaccgacgc aacgaatacg gcgtttcaca aaataaattg gctacggctt gtggacttag
     8701 tcggccgtac ttaaatcaga tagaaaatgg tggcgtaacc gcatctataa aaaccatgag
     8761 gaaaattttt gaccaactgg aaagctttaa tcccgatttg cctttgacgt tactgtttga
     8821 ctatgtaagg attcgctttc ccacaacgga tgcacgaaaa attattcaag aaattctcca
     8881 tttgaaattt gagtatatgc tccatgaaga ttatgccttt tactcctatc aagaacagta
     8941 tgtcatgggt gatattgtgg tgatgttgtc tcatgaagaa gataaaggtg tgcttttaga
     9001 attaaagggg cgaggttgcc gacaatttga aactttttta ctcgctcaaa agcgtagctg
     9061 gtacgacttt ttcgaggatt gtctaaaggc cggtggcgtg atgaaacgct tggacttggc
     9121 aatcaatgat cgagtggggc tattggatat acctgagcta acgaaaaaat gtcagaagga
     9181 agaatgtatc tccttgtttc gtacctttaa aagttatcgt tccggggaac ttttaaaggc
     9241 tgatgaaaag gacggcatgg gcaatacctt atatatcggc agtctcaaga gtgaggttta
     9301 tttctgtttg tatgaaaaag actatgaaca atatatcaag ttagggattc cattagacca
     9361 aactgagacg aaaaatcgtt ttgagattcg actgaaaaat gaccgtgctt ttcatgcgat
     9421 tcaagatttg ttaaaaggtc gtagcattga gagcaccacg ttttccatta tcaatcgtta
     9481 cttgcgattt gcggataaag tagaaggtaa aaggcgaacc aactggccct tgaatgaaca
     9541 atgggggcgt tttattggac ggaatcggaa agagattcaa ttaacttccg agccaaaacc
     9601 gtataccatt gaacgaacct tgaattggct aggccgacaa gtcgccccta cgtggaaaat
     9661 ggctaaggag ttggatcgat tgaatcggac aacctatatc caggatatgg tacagaatgc
     9721 acgcttatca gaccgacata aaaagatttt ggagcaacaa agtatggcaa ttgaaaattt
     9781 gattatatga aaagaggaat cgtcatggga attgtaaaag atattttttt ggtatttggt
     9841 ggcagtatgc ttggtgttac tgtgatgtgc ttgatgaatg caagtgcagc agcggatcga
     9901 gcaatggaaa agaaaggaaa gtgacaagat gaatttcgga caaaatttat gtgcgactcg
     9961 ttccttagtg aaaagctaag gcactaataa aagaacgaaa aagtttggtt attaggagaa
    10021 ctgataatct gacaggtgga atgatgaagt aacgctctga aacgcctgcc ctgagcctcc
    10081 gacaagcatt gtat
//
LOCUS       DAZKKW010000089        10056 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.091, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000089 DAZKKW010000000
VERSION     DAZKKW010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10056
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.091"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            272..541
                     /gene="rpsN"
                     /locus_tag="ACG8PO_002256"
     CDS             272..541
                     /gene="rpsN"
                     /locus_tag="ACG8PO_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFQ0207549.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            556..735
                     /gene="rpmF"
                     /locus_tag="ACG8PO_002257"
     CDS             556..735
                     /gene="rpmF"
                     /locus_tag="ACG8PO_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFQ0207550.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            767..1144
                     /locus_tag="ACG8PO_002258"
     CDS             767..1144
                     /locus_tag="ACG8PO_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0207551.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            1165..1314
                     /gene="rpmG"
                     /locus_tag="ACG8PO_002259"
     CDS             1165..1314
                     /gene="rpmG"
                     /locus_tag="ACG8PO_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFQ0207552.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            1338..2321
                     /locus_tag="ACG8PO_002260"
     CDS             1338..2321
                     /locus_tag="ACG8PO_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFQ0207553.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKYYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            2351..2473
                     /locus_tag="ACG8PO_002261"
     CDS             2351..2473
                     /locus_tag="ACG8PO_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HFQ0207554.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            2491..4014
                     /locus_tag="ACG8PO_002262"
     CDS             2491..4014
                     /locus_tag="ACG8PO_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HFQ0207555.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNELHNWPTFYPASLSKHEIAQEMMAH"
     gene            4038..4277
                     /locus_tag="ACG8PO_002263"
     CDS             4038..4277
                     /locus_tag="ACG8PO_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0207556.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(4470..4931)
                     /locus_tag="ACG8PO_002264"
     CDS             complement(4470..4931)
                     /locus_tag="ACG8PO_002264"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFQ0207557.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(5126..5344)
                     /locus_tag="ACG8PO_002265"
     CDS             complement(5126..5344)
                     /locus_tag="ACG8PO_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0207558.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            5462..7078
                     /locus_tag="ACG8PO_002266"
     CDS             5462..7078
                     /locus_tag="ACG8PO_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFQ0207559.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKEIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEGCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
     gene            complement(7319..8659)
                     /locus_tag="ACG8PO_002267"
                     /pseudo
     CDS             complement(7319..8659)
                     /locus_tag="ACG8PO_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(8935..9090)
                     /locus_tag="ACG8PO_002268"
     CDS             complement(8935..9090)
                     /locus_tag="ACG8PO_002268"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HFQ0207560.1"
                     /translation="MPTVIHSDRGSQHRSFIYQELLNDHHITHSVSQTGTPVDNAVIE
                     AFHRSIK"
     gene            complement(9097..9243)
                     /locus_tag="ACG8PO_002269"
     CDS             complement(9097..9243)
                     /locus_tag="ACG8PO_002269"
                     /inference="COORDINATES: protein motif:HMM:NF012870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0207561.1"
                     /translation="MMDITYIPCNDGRLYLSTYIDLATRILRYYMVDSHMKKEIVVRP
                     LQIY"
     gene            complement(9282..9476)
                     /locus_tag="ACG8PO_002270"
     CDS             complement(9282..9476)
                     /locus_tag="ACG8PO_002270"
                     /inference="COORDINATES: protein motif:HMM:NF024673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFQ0207562.1"
                     /translation="MFYEHKDRYGSPGITQKLYDEGIEINKRVVAVLMREMNLCTRGF
                     HRRKSSHGREKTIKEMVKKC"
     gene            complement(9386..9718)
                     /locus_tag="ACG8PO_002271"
     CDS             complement(9386..9718)
                     /locus_tag="ACG8PO_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFQ0207563.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            complement(9864..>10056)
                     /locus_tag="ACG8PO_002272"
     CDS             complement(9864..>10056)
                     /locus_tag="ACG8PO_002272"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0207564.1"
                     /translation="KNNFCHSLTPKITLFTYEVGRRSNPELGYGTSTRTSPLELKSMR
                     FTFRNDGRFIQLCVQSKFV"
BASE COUNT         3335 a         1599 c         1994 g         3128 t
ORIGIN      
        1 ggaaaagcta cttaaaacgg tatttgtgtt tgattggact aataaaagtg aattcttatt
       61 tttcagagcc taaactgcat attcttttaa atgatttgat tatctctatg taccgataaa
      121 ttcgttttca aatagttatc tattcatatg agttttattt catgttattt attgttgaca
      181 agagcaactc ttgttggtaa agtatcagtg tcaaaacgta acgattacga tttgaggtgt
      241 ctaaatcaaa ataaaaggag tataaaggta tatggcaaaa aagtcgaaaa ttgctaaagc
      301 aaagaaacaa atggcgatga ttgaaaagta tgcagataaa cgtcaggaat taaaagccgc
      361 aggagatcgc accgctctcg ctaaattgcc aagagattca aatccgaatc gtctgaggct
      421 tcgtgatcag acggatggtc gtccaagagg gtatatgcga aagttcggta tgtcccgtat
      481 taagtttaga gaacttgccc atcaagggtt gattcctgga gtaaaaaaag caagttggta
      541 aaaggaggaa atatcatggc agtaccagca agaaaaacat ctaaggcgaa gaaacggtta
      601 agaagaactc atcaaactgt agtaaaacca gaaatttcat ttgacgaagc gaatggtgat
      661 tatagacgta gccatcatgt atctttaaaa gggtactata atggaaagaa agtaattaaa
      721 ggagcttcaa aatagtttaa tatataactt ttaggaggga atcaaaatgg aaattattta
      781 cccaccttta gttgaagaag gattaaagta ttatcttgaa acgacccagc aatcgctaga
      841 taaatgcact ttttatcgtt cgatggtaga gagaggaatt attactgaaa cgggtttacc
      901 gacccaacag gcaattgaaa atggattagt caaagattac tatgaggacc aaggcttatc
      961 atttgatgat tttttaagaa tctatccaat ttttgaagag tatgacgagg agttatttca
     1021 atgtatcgat ggttattggg agatacctgt tgatatgaaa gaaaatttag tttcacaatt
     1081 ggaatctggc gagttaactt ttgaagatgc acaacaaatt caagcatatc ttgaagatcg
     1141 ataagtattg aaggaggaag atttatgcgt caaaatatta ttttggaatg tgtcgaaact
     1201 ggagaacgtc tgtatctcac cagcaaaaat aaacggaata accctgaacg gttagaatta
     1261 aaaaagtatt cgccaaaatt gcgcagacgg gcaattttta aagaagtgaa atagctaagt
     1321 ggtaaaaagg tgacaaaatg ggcataccag ttacaatcgt ttctgggttt ttaggggctg
     1381 gaaaaacaac gctaatcaat caagccttgc aagtctcacc ctaccccagg gaagaaatta
     1441 ttataattga aaatgagttt ggtgaagttg ggattgatca caaattatta gttcatagcg
     1501 aggaacgggt gttacaacta aataatggat gtatgtgttg cagtcttcgc agtgatttat
     1561 tagcaatatt gatttcttta ctggaaacaa tagaagaaca tcaacaacca atttcacaaa
     1621 tcatcattga aactacaggt atagcagatc cccaaccaat cattcaaaca cttttaacag
     1681 cgcctcaact aagaggaaga gtatatatcg atagtttatt gactgttatt gatagtgaca
     1741 gttttgaact tattgaaaga gaaccacaag cccttaagca acttgtcatg gcagatcgca
     1801 tttttgtgtc tagaaaacaa aacttgaatt tatctgcact aaaaaaatta cagagagaaa
     1861 tcaaaagtat taacccttta tccgatattc gaatgttcac acatagtgaa acgattcaaa
     1921 aaaaggattt ttataatttg aaaaaattta atcatccctt agtttttgaa agtgattcat
     1981 cagaaaatca ctataatggc aaatactatc atcaacacca tcaagtacat gagtttaaat
     2041 caattctctt aacttcagat caagacataa aaaaagattt tttactccaa tggattgact
     2101 ggctgatttt aaccaaccaa gggagcctgt atcgttttaa aggattgatt gctttacaag
     2161 agcaggattt tatagttgcc atgcaagagg taaacgaaca ggttaatttt caatacacca
     2221 ctcgacaaaa agaccaaacc actattattc taatcggaaa aatgttggat gaagaaagga
     2281 tcaaacagag ttttgataaa ttactggcgg aaagcaaata atgataagta gtgattgggg
     2341 gtaatttcta atggagaaaa cggatatatc tagtgcatat cgacgactta aaagcccgaa
     2401 tattaaaact agaaaaaggg cattaaaaat tatcaaagaa cataaacgaa ataaaatgaa
     2461 gaaattagca taattaagga aggaaaaaga atgaagaaaa tcagtgtagg acttattggg
     2521 atagcagctc taggattatt gggagcttgc tccagcacta atgatgcaaa agtatcgaat
     2581 gataaagatg gaaaattaga aattgtaaca acattctatc caatgtatga tttcaccaaa
     2641 aacatcgtcg gcgatgaagc gaacgtggat ttaatggttc cagcgggatc agaacctcat
     2701 gactatgagc cctcagctaa ggatatggca aaagctcatg atgctgatgt ttttgtatat
     2761 cataacgaaa acatggaatc atgggtacca aaagctaaag aaagttggaa gaaagctggg
     2821 cctaatgtag tagaaggaac aaaagacatg attttattgc caggtagtga agaagaagat
     2881 catgatcacg gagaagaaga tcaccatcat gaactagatc ctcatacgtg ggtttcaccc
     2941 aaaatggcaa ttaaggaagt ttcaaatatc aaggatcagt tagttaaatt gtatccgaaa
     3001 aaagcaaaag tatttgagac aaatgctgaa aagtatttga ctaaactgaa acggttggat
     3061 gctgactata ccactagtct taaagaagcg aaacaaaaaa gttttgtgac gcaacacgca
     3121 gcctttggat atttagcttt ggattatgga ttgaaacaag ttcctattgc tggactaagt
     3181 cctgaagaag agccatcttc tggtcgatta gctgaattaa aagagtatgt taaaaaaaat
     3241 aagatcaatt atatttactt tgaaaagaat gctaatgata aaattgcaaa aactttggcc
     3301 aacgaagctg ggataaaatt ggaagtactg aatcctcttg aaagtcttac aaaagaacaa
     3361 atggataatg gtgaagatta tgtttcagta atggaagaca atctaaaagc gttagaaaaa
     3421 acaactatgg ttgctggtga agaggtagtt ccagaaaaag aagcgaagga tgaaaagaca
     3481 gttgctaatg ggtactttaa agatgccgat gtgaaggatc gcgaattgag tgattataca
     3541 ggcgaatggc aatctgttta tccgttgtta aaagatggaa ttttggatga agttttcgat
     3601 tacaaagcaa aaatcaataa agacatgaca gctgccgagt acaaagatta ttatactact
     3661 ggctataaga cagatattga tactatcaac attaaagaca acaccattga ttttgtagtc
     3721 aacggtgaac atcatcaata cacatacaaa tatgttggat acaaaatttt gaattatgaa
     3781 aaaggaaacc gtggtgttcg ttttaatttt gaaactgatg atgcaggtgc tggtcgtttt
     3841 aaatatattc aatttagtga tcatggaatt gcaccaagta aggcagagca tttccatatt
     3901 ttctttggtg gagaaagtca agaaaaactc tataatgaat tgcataattg gcctactttc
     3961 tatccagctt cattgtctaa acatgaaatt gctcaagaaa tgatggctca ttaaaattca
     4021 atttttggag gaaatacatg gtaagaaaag tattattgtc tatggattcc aagaatattg
     4081 atgcttatcg gcgttatcga tttgcaaagc aaatgagatt aaatgggagt acattagctg
     4141 atattgctgc aactttacat gttagtattg acacagttca tagagtatta aaacgggatc
     4201 ctgattgttg ttttatcaag gtcaatactg gaagaagtag acgtttagca aaacggcata
     4261 atagcttggc caattaattg ataataccag agtagatcat ttttccctta tttggatttg
     4321 atctgctttt tattatgtct tgaattgtca aatcaagcgc aacactttat caaagaagac
     4381 ttcataaggt gttctccaat ctaaacactt tctaggtctg ttattcagtt tttcaatcat
     4441 ctgatttatc gttgagttat ctaccgtagc tatctctttc gattttggca agtattctcg
     4501 aatgagtcta ttcgtatttt aatttgttca ttgttgccaa gggcatgagg atctggaaaa
     4561 taaaagggca tattattcaa tgcttctgta actgcgaaat gattggagaa ttcttttcct
     4621 ctatctggag tgatcgtttt gaccgcctca tttggaatac tgcttaaaag ttcaatcatt
     4681 ttctcgctca cataagcggc tttctttctg gggaccttct ttcctgctaa aaggaagcat
     4741 gattttctgt ctgtcaaagt cactaaacaa gtaccaccag ttttgccagc tacaatatcg
     4801 gctttccaat gaccaaaatc acttctatca tctgcggatt ttggacggcc atggatcgaa
     4861 tgagtaaggc aaatctttcc acgagactcg acatgatttt ttgtatgtct tgtttttcct
     4921 cgatggtgca atgaacggat caggccttta ttgccgtgtg agaggggttc tgtatcgaaa
     4981 agatcattgc aaattgcacg ttagatcgtg ataaaactaa ttgaaaagtc catatgttca
     5041 tgcttcaaac gattcgagat ttcctctggc gaccactgaa aatcgacaaa taaatgacga
     5101 acaattgacc aaactcacgg atcatttaga atgtgataac gtccgcattt ttttcttcga
     5161 taagcataat ttttttccgc tttggaaggt gaatagtttt tttcttgttt cttctcagtt
     5221 catgtaaaat agtaaacggg ctatgctgaa tgtcgttgcc aattatacta atagaaaatc
     5281 cttcttcgtg aagcagaaag attctttctc gttcgagtat agtaagatga cgatactggc
     5341 tcataattat tttccttcta ggagtgttct cgacaaactt atactagttg gatatcgatt
     5401 atgactcatt tttatgtgtt gcacttaaag tgtaaattca aggtatagaa agaggttccc
     5461 tatgaaaaaa atacctatta caatcttaac tggctatctt ggttcaggaa aaacgatact
     5521 tctaaatcat attctgtacg gtgatcacgg aaaggaaatt gctgtcattg taaatgacat
     5581 aggggaagta aatgttgatg gggtattagt cgacaagaaa ggttttaaaa gaactggaga
     5641 aaaacttgtt tcgatgccga atggatgcat ttgttgtaca ttacgcgaag atttgattgt
     5701 tgaactagat caattagtgc aattttctga cttgaatcaa attgttattg aagcttcagg
     5761 aatcagcaaa ccaattccaa ttattcaagc aattatatta gctaaaaacc ctttagacat
     5821 tgatttgacc caacgattag agattggtgc cattattata gtagtggatg taaaacgttt
     5881 ttgggataat ttcgaaacgg gtgaaaagat tcaggaaaga aaagctgaag gtaatgtagt
     5941 tgaagaagaa caggatattc gtggcttgtt aattgatcaa ttcgagtgtt gtaacatatt
     6001 acttttaaat aatagtgact tagtgtcaac caagacgatt tacaaaatta acggtctaat
     6061 tgctaagtta cagtccaatg ccaaaataat taggacaatt aaaggtgaag tagacttatc
     6121 gaaaattcta aatacaaatc tttttaatat agatgagact tcctattcag tgggctggtt
     6181 caaagagtta gagcttggct atcaaaatca tattcctgaa acagaagaat atggtatctc
     6241 aagttttatc tatcgaaatc ggttactcct tgacgcacaa aaatttgccc atcttacgga
     6301 taaaaatttt cctggcaata ttacaagagc aaaagggttt ctttggtttg cccaacatag
     6361 tgatttttcg acgatctttg aaacatcggg tagctataaa agtattgaat cattaaatta
     6421 ttgggtagct agtttaccta aaattgagtg tcaaatgatt atacaaaaaa atcccgaatt
     6481 gaaggaagcc tggcatccgt tgtttggtga acgcattaat aaactagttt ttattggtat
     6541 gaagatggat caacaatgta ttaaggaaca acttgagggt tgtttaactg attctcttaa
     6601 tgcagataat ttccgaaaaa tttcaccatt tccatggaaa aagtctaata tgtttagcca
     6661 gatatcgtta atgaaatttt taagaaaagc tggtgtcaag ataggcgatg tcataattat
     6721 tggcattttg attattacct cttttattcc cttatttgta ttctctatag aaaaggagaa
     6781 tgaaacaact gaagatgcta tcaaatatgc catcgttcga attgatggca aggaaatgga
     6841 tcgcttcgat ttggataaga tcaatcataa actcgtgaat tatcatcctg cgaagggaca
     6901 atataatatt gttgagatta aagatggaag aattcgagtt aaagaagata atagtcctga
     6961 tcaaatcgcg gttagaactg gatggatctc aaaacccggt caaacgagta tttgtttacc
     7021 ccataagtta gttattagta ttgagaaaaa agaaagcaca gattactaca tttactaatc
     7081 tggaaataga tgaggaggtt tttaataaga aaaagatatt attattaata atagcagggg
     7141 ctttattaag tgtgttaagc gcttgtggac aagaaaaaga agcggtgaca acaggcacaa
     7201 gtatctttaa cgggacctgc aatggtttag ttaacagtcg gttagattac ttattttggc
     7261 tcttcgtcaa tgagtgttgg tgaagaaatt caatagaata atcgagatac tcacacggct
     7321 agacagctat cttgtctagc cgtgttgttg ttctggcttt agtcagttgg gtagtattct
     7381 tagttttctt aaaatatttt cctcgatata aaaagattcg ttctcggtag ttttgaaagt
     7441 ttctatagcc ataggcaatt cgttttagta ctttgatatg gttattcaaa cattctatag
     7501 ggccgttgga atagggaacg cgcaacgctc gtttgatttc tgtttggtat ttcttgaagg
     7561 tcgtgaacac tggttggtag tgctctggca actccttgta gtcttctttt aggagcgcga
     7621 cgaattcagg aacatcttgt cttctgataa cggaaagaaa atcctggtag acttcgtagc
     7681 ctcgctttaa ggaatcatca taagataata gccgatcgac tatctctgct tctgtcagat
     7741 gatctcgaaa tgacggacgc cagtagcgat gctgaccttt caattcccac gcattctttt
     7801 gaagtagttt ccagtatttc tttaagtgct tcgaccgacg atcccctttt ttaaaggtct
     7861 tcattacttg aattcggtgg ttggtaaaag cccgaccaat gtgttggcta atatgaaagc
     7921 gatcggcgac gattcgggca ttcggaaaac actctttcac caatgtgcga tagggtgtat
     7981 agaaatctga aaccactaga cgcacttgtt cacggacttt tatagggtag cgcaagaaat
     8041 agtcgcgtaa aggattgagt tgccgatttt ccacaatatc aatcagttga tgggtcttgc
     8101 cgtccatcat gacaaagctc atggctcccg agacattttt cactgatttg aattcatcga
     8161 aacacaccac ttctggtaac agctggctca aagaaatggt tttaggccga aggctgcgaa
     8221 gtattcgaat gacagtggtc ggagaaatgt gcttcatacg tgcgatcaaa gacatagaaa
     8281 gaggttcgct taatagctca agaatggctt gtttgactct gcgagcaatg gagcagcgac
     8341 gatcactaac tgaatcttct gctaaaaaag tctttccaca gtgtttacat ttgaagcgct
     8401 gtttgttaat ccgcagaatg gtcttgtagt tactgacatc attcaataag atggatactt
     8461 ttttccagcc ctatttaatg atcgtttgtt cattttgata gtggcgatga aagcattcct
     8521 ccggttgata ggtcaatgtg ccatcaaaaa caaaacatgt ttcgtcattg attcgctctt
     8581 tgcgaaaggc atcttcatga aagataagat ttaggtctag aatatctagt aactctttgg
     8641 taaactctga ataggacatc ttgaaacact cctttgaatg gttttcgtcg actttattct
     8701 aaaggatttt aaggtgtctg tttgcgttta ttagaaaaaa gaaaaagtat tggcaacaga
     8761 aggcgtcacc aacactagat attatagatc ccttattttc ttcgcttcaa attgtcaagg
     8821 agtcatcata aattttgtat ggattctttt gtttgggtag tagtcttcta aataatcttg
     8881 gattaacacc ttcatctcta ttttactttt atatttgttg gagtcgatta attctcattt
     8941 gattgatcga tgaaacgctt caattactgc attgtcaaca ggagtcccag tttggctcac
     9001 tgaatgtgtg atgtggtggt cgttcaacag ctcttgataa ataaaagacc gatgctggct
     9061 acctctatcg gaatgaatta ccgtaggcaa ttctccttaa taaatttgta atggacggac
     9121 gacaatctct tttttcatgt gtgaatctac catatagtaa cgtagaatac gagttgctaa
     9181 gtcaatatag gtggacaaat agaggcgacc gtcattacat ggaatgtaag taatatccat
     9241 cacccaaaca gcatcaattt ggttctgttc aaattatcga ttcaacattt ttttaccatt
     9301 tctttgattg ttttttctct accatgagaa gatttgcgtc gatgaaatcc tcttgtgcat
     9361 aaattcattt cacgcataag aacagctaca acacgtttat tgatttcgat tccttcatcg
     9421 taaagttttt gagtgatccc tggagatccg tatcgatctt tgtgttcgta gaacactcgt
     9481 cttcctcttt tagtcgttta ttttcctgtt caagaatgta gaaatctgct ttatcaggta
     9541 atctttttcc ttgtccagca aaagcatttt tcccgtatcg acgatattct gaaacccatc
     9601 gggaaattgt tggacgagag atattgtatt cttcagcaat tttcttggta gttacttttt
     9661 tctcaaggat atcctgtgaa atctttagtt taaatttacg atcatatgtt tttctcaata
     9721 gagttcattc cttccggtaa cgtaactgat agtcaagaaa agtgtatcat agcccaactt
     9781 agtatcaggg tttgtcaact aaactgtgga agttgataga taagagtttt taacaaccaa
     9841 aattctcttg actatttttg aaatcagaca aattttgatt ggacacatag ttgaataaat
     9901 ctaccgtcgt tacggaaggt aaatcgcatg cttttcaatt ctagaggaga agtcctcgtt
     9961 gaagtaccat agcccagttc tggattcgac ctcctgccca cttcatacgt aaataaagta
    10021 attttaggag tgagactgtg acaaaagtta ttttta
//
LOCUS       DAZKKW010000090         9684 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.092, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000090 DAZKKW010000000
VERSION     DAZKKW010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9684
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.092"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            191..1189
                     /locus_tag="ACG8PO_002273"
     CDS             191..1189
                     /locus_tag="ACG8PO_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFQ0207565.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            1301..1810
                     /locus_tag="ACG8PO_002274"
     CDS             1301..1810
                     /locus_tag="ACG8PO_002274"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HFQ0207566.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(1840..2499)
                     /locus_tag="ACG8PO_002275"
     CDS             complement(1840..2499)
                     /locus_tag="ACG8PO_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HFQ0207567.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(2501..3265)
                     /locus_tag="ACG8PO_002276"
     CDS             complement(2501..3265)
                     /locus_tag="ACG8PO_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HFQ0207568.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(3273..3950)
                     /locus_tag="ACG8PO_002277"
     CDS             complement(3273..3950)
                     /locus_tag="ACG8PO_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HFQ0207569.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(3987..5372)
                     /locus_tag="ACG8PO_002278"
     CDS             complement(3987..5372)
                     /locus_tag="ACG8PO_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HFQ0207570.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(5582..5666)
                     /gene="ffs"
                     /locus_tag="ACG8PO_002279"
     ncRNA           complement(5582..5666)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACG8PO_002279"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(5689..6204)
                     /gene="tadA"
                     /locus_tag="ACG8PO_002280"
     CDS             complement(5689..6204)
                     /gene="tadA"
                     /locus_tag="ACG8PO_002280"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HFQ0207571.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            6311..7168
                     /locus_tag="ACG8PO_002281"
     CDS             6311..7168
                     /locus_tag="ACG8PO_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0207572.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYRYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDGTEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(7249..7584)
                     /locus_tag="ACG8PO_002282"
     CDS             complement(7249..7584)
                     /locus_tag="ACG8PO_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0207573.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(7651..9021)
                     /locus_tag="ACG8PO_002283"
     CDS             complement(7651..9021)
                     /locus_tag="ACG8PO_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HFQ0207574.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(9099..9635)
                     /locus_tag="ACG8PO_002284"
     CDS             complement(9099..9635)
                     /locus_tag="ACG8PO_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0207575.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
BASE COUNT         2843 a         1998 c         1620 g         3223 t
ORIGIN      
        1 actaatattt gcatacttca taataataat aacataatac ctaacctctc gtaaaaatac
       61 tagatttgtt ttcttttatt tttcatcact agatacattc tataacttac cgtttcgatt
      121 tcgaattcaa acatttttga gaagcattct caattaaatt ctgctatagt gtgaaaaggg
      181 aggaattttt atggaaatct atgacattac aatcatcggt gcaggtcctg tagggatgtt
      241 tgccgctttt tacgcaggaa tgcgccaagc taaaacaaaa atcattgaca gtctacctca
      301 acttggtggt caattagcaa ctttgtatcc cgaaaaatat atttacgata ttcctgggta
      361 tccagcaata aaagcaagtg aattaatcga tcaattagaa aaacaattaa ctacttttaa
      421 ccatacattc catttaaaag aagaagttct ctcgcttact cgtgaagacg aagttatcga
      481 gattactaca aataaaggca tccattattc caaagcagtc attttagctt taggaaatgg
      541 ttcattccag ccaagaaaat tgaatttaga taatgctgaa tcatttgaaa accacggatt
      601 ggattatttc gtgaatgatt tgatgaagta tgctggaaaa aaagtcgcta ttgctggagg
      661 tggtgattct gctatcgatt ggacgttgat gttagagcct attgcaagcg aagtttactt
      721 gattcatcgt cgcccggaat ttcgtgcgca cgaacatagt gtatcacgct tgaaatcttc
      781 atctgtcaac ttgcttactc cctatctcat cgatggatta tcaggcaata atggggaact
      841 gacagatatc cggttaaaaa aagtgaaaag tgacgagacg attgatttga tgattgattc
      901 tctgatcgtc aattatggat tctcttctaa tttagaacat ctttcttctt ggggacttga
      961 tagtaccaga aatgccatta ctgtaaaatc agatatgtct acttctattc caggcgttta
     1021 tgcagctggt gacatctgta cctatgaagg aaaagtgaaa ctgattgcta ctgggctcgg
     1081 tgaagcacct actgcagtga acaacgcttt acactatatt aatccaaagg aacgaactca
     1141 gcctggtcac agcacaagct tatacgataa aaatcttcgt cctagttgat tttttatatc
     1201 tctttgcatt tcaagttaaa cccgaaagca gtttcttgca tttttttcat gttccgttaa
     1261 catgaaatag agaaagaaaa aataaagggg gagcaataat atggtcgtca aaactgagac
     1321 tttagaagaa aaggcaagag aactattgaa ggaacgtggc gtaactgtag aagatatcgc
     1381 acaacttgtc atgtttttac aaaaagacta tatttctgat ttgactttgg cggattgtac
     1441 tgaaagtgtc aacagcgtgc taacaaagcg tgaagtacat aacgccatta tcactggaat
     1501 ccaattagat atattagcag aaaagaaaaa attgatgagt ccgttgcaac atattatcga
     1561 agatgacgaa ggcctttacg gtatcgatga gatcctagcc ttgtcaatcg tgaatgttta
     1621 cggtacgatc ggctttacga attatggtta tatcgacaag gtgaaacctg gtattttaaa
     1681 agaactaaac agccacgatg gcgttcatgt ccataccttc ttagatgata tcgtcggagc
     1741 gatcgccgct gctgctgcca gtcgattagc acacaaagaa ccagaaaaaa gaagtcgcct
     1801 tactcaataa ttcaacaaaa aaagaaagaa aacgtctatt tagacgagtt ctttcttttt
     1861 tgtttgtttc aagctatttt ttcctaagaa aagaaaaaca gcaaaaaata attctaataa
     1921 tacaaaaaat aagataaagc tatgcatgaa aaattcttct ggaagtacat tgatgattgg
     1981 atcagtaacg ggatcgaata accagtcatc gttgttgaaa aacgtttcat gaaaaaggat
     2041 aaaaaagcga tcaaatccta tcatcatgaa aaaaccgaaa acaactggca gtaacatgcc
     2101 gatttgaaac ggacgaatca accgccaaag ccgatcattc ttttgcaaat atttgataaa
     2161 caaaatactc gggatcaaag tgatgaaaaa cacagcataa tccactaaaa acaaaatttt
     2221 cacttcataa aagtgatggg ctccgctagc agaaactggg aaatctggca agctgaggat
     2281 ggttttgaag ggattattga gataattcat taggtgatca aaatttttca gtaacgtctc
     2341 ttgatctaaa gaagtataat ccaaaatttg caaatgtccg atatcaaata cgtatagtgg
     2401 tcgaaagttg atcgtcacac caactgccag actgatcagt gtcagaaata ggctgaacaa
     2461 tccagcatat tccatccact gccatctcga atttttcata ttagaagctc cattcatcca
     2521 gtgattgttt cacataggta gctgggatag gcaacccagg tacatcttct tcttttgtaa
     2581 aacctgataa aacgagcaaa gtatctatat tattgcgtat cccagcttgg atatctgttt
     2641 catagttatc tccaaccatg attacttctt ctttttgcag ccctaatact tcaacggctt
     2701 tatccataat gatggcttct ggttttccga tgtagatcgg cggcgtttgg gttgctgtct
     2761 ctacaaaaga gatgactgac cctgctccag gaagaaggcc gcgttcagta ggtatattct
     2821 tatctgggtt cgttccaatg aaagtagcac ctttttgaat agcaagtgtt gctttaacta
     2881 ctttttcata ggtcagataa ttatctaagc ctaccactac atagtcagga gcctcttctt
     2941 cccatgtaaa tccggcagct aaaatcaagt caatcaatcc tgcttctccg ataacataga
     3001 cttttttccc ttttccatct gctttcataa aatcaattgt tgccaaagta gctgtgtaga
     3061 ctgtctctgg agctacatga atgtcaaact catctgctag acgattagct actgtttctg
     3121 gtgacttagt cgtgttattg gtaacaaaca agaaaggtaa ttttcttttt tgcaattctt
     3181 ccacaaatcg tttaccggcc ggtatgggtt gtgtcccacg ataaatcgtt ccgtctaaat
     3241 caattaaata tcctttatag tccattcatc cactactctt ccctttttct gatcgtaaat
     3301 gctgtttttg ttttttctac ttgtttaggt tgttcttgtt tttttacttt ttcagaggtt
     3361 tttgctgact ctgtttttcg atttttaata accggttttt tgcgattaga tgcgactggt
     3421 ccttttttct catcgatata cgcacgatta tggttttttt tcttttttcg gcgctgagtt
     3481 gtcttttctt ttttaccgcc gattctctcg atgacaaaat acgcacaacc aaaattgcaa
     3541 tattcgtaaa gatagtcttc caaggaatcg attcgctgat ctggatgcgc ccgtcgatta
     3601 tccgcttgga aaaatccttt taggcgtagt tgttcataac cccaatctcc tacgatataa
     3661 tcatatcggg ctagtacatc actgtaacgt tctccaagct tttctgcatt aaatccttca
     3721 cgataatcgg acacaagccg ataatgtctt tcacctatca aaaaattact gtcatctaac
     3781 agtgtcacta gctcgccttt gactttttct gctttaggct cctcaacgat ttcttccaaa
     3841 gcagctgtca gttcttcggt gactgttgtt tcctgttcag ctgatgattt tttcttagca
     3901 atttctgttt tatgctcaac gttctgctgt tcattttgtt ttgcagacat gcgttcacca
     3961 cctatctttt ttattatacc ttgtttttat ttgattgggt agtgagcttt caagtaatct
     4021 cctaccacac ttcggataaa ttcagggaac aaaaattcat tttttccagc aatctcaatc
     4081 gttggaaaaa atggaacaaa catcaaatga tctacggtag taaaagtata atttttattc
     4141 agatcgattg gcttgttctt ccaaaagact tgatggttaa tagaatcata tgtgattcca
     4201 ttatagacca tctgcccaaa aatcttaccg cgaaatccca ttcccttcat aggatagtta
     4261 cgtaaaaagt tacgattttt ttctatttct aaaactaagc ggatcagatc atttccaccc
     4321 aatgtgacat ttaacaagtg catcggatgc ggcaacgcag tgtgaagctg atcctgattg
     4381 acccgccctt ttggaatttc agttaaaaac agaccactgt ttaatattgc tgcttccgtc
     4441 tttcctcgtt ctttcaccgc ctcaaaggct acttggataa acgaatgctc attgaagata
     4501 tctagactca gagaaaatgg caaatctgct acttcttttt cttttaataa ttcatgccca
     4561 cgttctagat aactatttat ttcttgttca tcttctgtaa aagctgtcat tgtttcggta
     4621 gggatggctt tggctgaatg cttaaggatt tttttctctt catcgatggt taaatggact
     4681 tcaccgacat actgcccgta ctttccagcg gctgctattt ggacatcatt gattacgtga
     4741 ccttctctga ataaatgatg ggtgtgcgat cctaaaataa catcaattgc tggtatttct
     4801 tttgcaattt ggaaatcatc ctgaatcccg agatgactca tcaaaactaa aacatccact
     4861 tgtgaacgta aatcttcgac taattctggc aaaatatcat aaggattccg tatgtcccag
     4921 ccattcggat tataagtaag cggaaatgga gcagtaaatg caattagtcc gattttcgtc
     4981 tggtgtttcg ttgtaatgat tttatatggt ttcgtccatg ccggtggctg gagcgtattt
     5041 ttatcgaata agttatccag gatgatatca aactcagcat gatcgtataa atgattcaac
     5101 tcttctttgg aatttcctac gccttcattg tttcctatcg tcgctgcatc ataaccaact
     5161 tcattcatca gcaagatatt ttcctgtcca ttcgttgctt ctgacaatgg atgccaacga
     5221 tccacaaaat cacctagatc tactgcaatc gttgtcgttt tttctttctt ttcgttttct
     5281 cgtttacgtt gatccaaaaa ccttctgatc cttggccaat tttctaaatg agaatgcaga
     5341 tcattcgtat gtaaaatcac tattttttcc atgtaccctc tcgcctctct ttatctttat
     5401 cataccatag gcagcttttt cttccattga aacaagctct atttcagagt ttttcctgcc
     5461 ttgaagcaaa ttgtataagt acaatattcg ataaatcagt ctggctaaaa aatcaggaac
     5521 attcattttt acagtactat aaaaataaaa aaaacagacc cgaaagtctg ttgtcgtttc
     5581 attataatca gacacatggc ggaacctgct tagtgctgct tccttccaga cctgacacgc
     5641 ttcacaagcc caccattgtc ctagcctttc ggcaaagaat agtatacctt atttttcatt
     5701 cgttgaccag tcttttttta atttcttttt tcgctggcgg agctttcgga aaaagtcagt
     5761 caaaatcatc ccacattcgt cttctaaaat gccaccttct acatatgctt ggtggttaaa
     5821 ccgctcgtcc tccaaaagat tcatcaaggt accggctgtt ccgccttttg gatcatacgc
     5881 gccgaaataa acttctgcta cacgagacaa aatcatcgcg ccgctgcaca taggacaagg
     5941 ctctaaggta acaaaaagtg cagcatcttc caagcgccag cttcccactt tttcacaggc
     6001 ttccttgatt gcaagcattt ctgcatgtgt agtagcatct tgcgtggttt ctctaaggtt
     6061 atatcctctt ccaatcactt ttccatccaa tacaaccact gctccaatcg gaacttcatg
     6121 aagcatttct gctttctttg cttctgccaa tgcaaggcgc atccattttt ctttttctac
     6181 tgcatcaaga tttgtattta ccaattaccc ttccctgcct ttgttttttc ttgttttact
     6241 ttagcatatg ataaaaaggc tgtaactaac ttttttttct atggtaaaat aaatacaaga
     6301 ggtgaacaag atgaaaatgg ataaattact tgctctttac ccacaagcac agaaaaaaaa
     6361 ctctccagca tcagaagcgg atatcctttc ttttgcaatt gatgatgctt ttatctggat
     6421 caaacaagat tcattatctc aacaagaaac cagccttttg aaagcgttat tccctgtaat
     6481 caacgatcaa aaaaaacacc catggtatcg ctatcttttt aaagatgctt cttgtgttca
     6541 agaaaaaagc tttcgtttga tccagcttca cgtcgaatca aaaggagaat tcttaaaaaa
     6601 agaatggcaa gatactattt tggaattatt tcgctattct gctgatttct ttttttatac
     6661 agacacagat gcgctcttag tcgaacaaat aaaccgtgat tatcttgatg gtactgaaat
     6721 caacggtatt tttctatctt tagatgctga ttttgatacg acaacgtctg tattcgttgg
     6781 ttctttccat tcacctggca ccgacctcgt ccctttgttt gctgaagaaa gaaaaatttt
     6841 tttaacagag caaaacaatc tttatactca cagccgtact ttttctatgc aagatgtggc
     6901 tttacattac tatacaaaag atactgtaaa agatagtaag ttgatcaaaa gttacataga
     6961 actgatcaaa caaaatgaaa tggaagtgat cattcaagaa ttatggaatg aattaggcaa
     7021 tgtcagctct gctgcaaaaa cactgtttct tcatcgtaat actctaaaat atcgaatcga
     7081 aaaatttcaa gaacagtctg gtttcaatct aaaaaaagcc aacgatttac tgttttgcta
     7141 tcttttattg cttcatgaac ataattaaag atagattaaa ggggttgtga caataatttt
     7201 gtcacaaccc cttatttcga atgaatagtg ttcaaaagct agtttctctt acatcatttt
     7261 ttcaacaaaa tgcattagcc ggtcttttac atcttcggtt gaatctttga ttgaatcacc
     7321 ataaacagag attttctttt ttcctgattt gatttttgct aacattccca tcaaagaatc
     7381 caagtcacta tctgaaagac catctacgat ttctagtaat ttttcatttc catcaaattt
     7441 gtcatagacg aattttttta ctttcgtccg agtagtcata tgatggatct tttccattac
     7501 ttttcccgat acaacgactg ctgtcgctac actggcagcg gctgctacgc ttaatccgat
     7561 tgttactttt gttgatgttt tcataagtat cagctccttt ttatctatct ccatcatagc
     7621 atagacaaaa aaataaacga actaatccac tcagtgaaat ctcaatcctt tagggaaaaa
     7681 gttctttact gttcctttta cttgtttccc aaatccgaca ggtatcttat ctaatactag
     7741 caaaacccat ccttgattcc catcacgttg aaatgtttct cctgctgtgt aagattgcca
     7801 ttccttttga gtgatcggca agcaaggtat attctctatc ttttttgtag caagcgctaa
     7861 tgcatagcta ggttcaaatc gattcttttt gaaatctcct aaatgcaaac ctgtacgtac
     7921 gacttttaaa ccatcgagag aaggggctaa ctcaggaact tcccaaagat ggtcgttgaa
     7981 cactaaaagt cttccagtag cttcataatg aaaatcattt gaaaactccg tccagagttt
     8041 ttcctgctct tttgtcattt gcactttact tttttttcgt gtcttctttt ctttatgcat
     8101 ctgattttgt ccatgaaaag tcagctttgc aacaaaatgt ccttcaccct gatctttatg
     8161 aggccatata cgtattgttt tttccaaacc agctacgctt ccccattcag aacgtcctga
     8221 actaacggat tgagtcaaag gaatctcctc aatcgttact ggataatttt ctacaagcca
     8281 gctaatgatc tcttcatttt cttctggtgc aaacgtacaa gtcgaataaa tcagttgtcc
     8341 tttatttttt agcattttta ttgctgaaga caaaatctct tgctgacgtt tttgacagta
     8401 tagcggagat tcttctgtcc actcttttat tgcatttgga tcttttcgga acattccttc
     8461 tcctgaacaa ggagcatcaa ccacgatccg atcaaaaaag cccgaaaaat gagggacaag
     8521 ttctgcgggt gcatgatttg tcactattgc gttcgaaact ccccatcgtt ctatattttc
     8581 tgataaaatc ttcgcacgtt tcggaaagat ctcattcgtc actaataacc ctttcccctt
     8641 catttgagca gctaattggg ttgatttacc accgggagcc gcacacaaat ccaaaacttt
     8701 ttcaccgggt tttgctgcag cagctgttcc aacgatcatt gcacttggtt cttggctata
     8761 ttcataccca gcttgatgga ggaaagactt tccattgaca gtgccaagaa atccctcatt
     8821 gctgtaggga gcgggttgta attcttcgct atggtaagtt tggaccatgt ccaatcctgc
     8881 cggttttagt ggattccacc gaaatccttt tttcacgctt ccttgttcaa gcgcagaaaa
     8941 aaaatcggat gcttcttcgc ctaaaagaag acgatatttt ttgataaatt gctgtggtaa
     9001 cgttgtcgct tcctccttca ttccaatgtt tcgttagaca ttttacaatc ttttttacga
     9061 aaaataaagg tggctgatct actttttcat gttgctgcct aaggtgactg ttcctttaag
     9121 atattttcca agtattcatc cgtcacctcg tttttatccc aaacagcgtt catcatttca
     9181 agaaaatctt gagaagaatc atctgagcgc aaacttccca atgcattaag ccataccaat
     9241 tcaagcgact tttcttcttt gatcttcgtg tgacctttga catatgagaa aaatggccgc
     9301 ttaaatagcg aatccatgtc taaatcagat tttgcaatga tttcacgaat actttcatgg
     9361 atagaaaaaa aatctttcaa taagtagaca atgcttaaaa catcatcgga actgcattca
     9421 gctaagtagt tttcaaaata aacagtgatt tcttctaata tcttctcttt taacccgctg
     9481 attgatttga aatgtgtata aatcgggtat gtagaacaac cgcagaactc tgctaactct
     9541 cttgcagtaa ttgacttcat tccatattct tccacgtata ccatactttt gaacagcaaa
     9601 tattctttac tataatttgt tactctcccc atcattctta cctccgtctc ttttcatcta
     9661 cccataaata aataacataa ataa
//
LOCUS       DAZKKW010000091         9660 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.093, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000091 DAZKKW010000000
VERSION     DAZKKW010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9660
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.093"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..613)
                     /locus_tag="ACG8PO_002285"
     CDS             complement(<1..613)
                     /locus_tag="ACG8PO_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element IS1476 family transposase"
                     /protein_id="HFQ0207576.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGKLDDSP
                     SYCEHCRSVRIIRNGSYTTRTQILKVKEKLTILELKRTRFLCHDCGRTFSAKTDLVDE
                     HHQLTKELKQAILMELYENQSRKLIAKKYFVSDGTVTRILREATKHYQSRMNFLPTVL
                     CMDEFKSMKSVSGSMSFICVDGTTHQLFTILEDRRLYKLTQYFL"
     gene            814..2028
                     /locus_tag="ACG8PO_002286"
                     /pseudo
     CDS             814..2028
                     /locus_tag="ACG8PO_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(2207..2542)
                     /locus_tag="ACG8PO_002287"
     CDS             complement(2207..2542)
                     /locus_tag="ACG8PO_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFQ0207577.1"
                     /translation="MKMNTNDIATVYVAFTAAEGGKRRPVYILKNGLDTISFLSITSK
                     YHTKSDKIKLKYVEIRDWKQAGLKKESWIDVGTLNELPKNNKKVKWKKIGELSPSDLQ
                     RLEERMEEL"
     gene            complement(2526..2789)
                     /locus_tag="ACG8PO_002288"
     CDS             complement(2526..2789)
                     /locus_tag="ACG8PO_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HFQ0207578.1"
                     /translation="MAKLQISMDEKLKNEAESIIDELGMSPKTAVTVFYKQIVEQGKI
                     PFSIELSEKSKRIHNIQRLAEKLPVQKLDTDEKIEEWFNEDEY"
     gene            complement(3249..4052)
                     /locus_tag="ACG8PO_002289"
     CDS             complement(3249..4052)
                     /locus_tag="ACG8PO_002289"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFQ0207579.1"
                     /translation="MRFLHLSDIHFRENYQTNLFTAELFPTIKNPQLALEKLLQELSL
                     TVYDFILLTGDLVHEGGISDYQVLRKMLDQYFPTTPYYFCRGNHDRRSVFFEGMSVES
                     NETGEYIHCTNQNGLRIISLDSAQDTQHEGKISIGQMIQLQEWLGTPAEKGTILLVHH
                     PLSWEDPSIVTEVPEGFAELIAESDILGIFVGHIHQGSSAEFAGKKQFMAESLSFGVD
                     EYPDYSLFTNRTGFTSGSLIDGKIFTYQHYLTPHQIEIGRMKKPITGNH"
     gene            complement(4030..5154)
                     /locus_tag="ACG8PO_002290"
     CDS             complement(4030..5154)
                     /locus_tag="ACG8PO_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0207580.1"
                     /translation="MGETLELRNIGKKYGEHTILKEIDLAVAAGERIVLLGPSGSGKS
                     TLLRMIAGLEKITSGELFLGTQLANKLPCGEREIAMVFQNYALYPHMTVKENIIFALR
                     ANKIKTADIQHRLEEALKMLGLTPYQDRLPKDLSGGQRQRVALARAVVKRSNYFLLDE
                     PLSNLDVRLRLDARKELVKIHQRYKQTFVYVTHDQIEAMTLADRIVLLNEGRIQMIDT
                     PKNVYTKPKNTFTANFIGSPGMNIMPVTYDQGTLKVASQFLTLNPAWQKQLLQVSGSL
                     YLGIRPEHVTFSKETSDLHGQVKYRELLGQSYAYTVKVDGIEMIVLNEKPTWQIGDAV
                     NLRIEPERMHFFDYQTQENLGYPDYFIEERGNSHALFASL"
     gene            complement(5170..5985)
                     /locus_tag="ACG8PO_002291"
     CDS             complement(5170..5985)
                     /locus_tag="ACG8PO_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0207581.1"
                     /translation="MKIKRWLPHLLFMFIIIIIIYPLLFIMANSFKPLKDAYQTITSL
                     IPQPFTLENFCYLFENLPIIKITRNTFFIATIITVVKLGVAFFAAYSFTYCRIKGKKI
                     WYFIIIGTMFIPFTVAMIPNYLLIAKLNLIDTTWGVIIPQFADAMGIFLLTQTMREIP
                     VSLLDVAALDNIKQTTVMSKIVFPLTRHAITSTGIWFFITSWNEYVWPVLILRTVDNY
                     TLPLAMQTYISSEGGTNFTVAMAISLITMFVPLVLYAIFQKYIIGTFVSAGIK"
     gene            complement(5975..6847)
                     /locus_tag="ACG8PO_002292"
     CDS             complement(5975..6847)
                     /locus_tag="ACG8PO_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013669610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0207582.1"
                     /translation="MKRYVGYLYLAPAIILLGVFVFYPLIYTIYLSFFDWNMVAPTKE
                     FVGFANYVKVLTDPVFHKVLKNTLSYTVLFVLLSCVFPYITSFLLGIVIEKGQNLYKP
                     LFFIPAVISLVVAGMVFTWILNPVSGPIAVLLGNFGLRLPMWMNLQGWVIAVIAGITA
                     WKVFGYNFIVIFAAIVGIDQEMIEAAKLDRVPIWRIFFTIVLPMSSSTGLYLLVMTIV
                     QGLQYVFTPIKIITRGGPNNGSANVIYYAYQKAFEMYKVGESSAISVLTLLIFAVFLG
                     VIFKFVEKGVYYEN"
     gene            complement(6932..8221)
                     /locus_tag="ACG8PO_002293"
     CDS             complement(6932..8221)
                     /locus_tag="ACG8PO_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0207583.1"
                     /translation="MLKKNLVIGVVSVLSVISLAACGSGNTKTTKKSEGRVTIEYWHV
                     NADTLGGKTVNELVDKYNQSQDKVKVVAKYNPDMYKGLMQNLQASVASKEIPDVVQVG
                     WAFKDYFAENFKYTNPQSLVEEVDPDNADYFKDHFLDNIMALSEKDGKQVGIPYSISN
                     PVLYLNQDILKAAGLDENGPKTWEEVQTFSKIIKEKTGKYGVYIQEPADNWAQQAILE
                     SNGASVIKDGKATFASKDGIAAYELYQRMVKDQTALHTTWEQGVQSFIDGNVAMVYTT
                     IAQRDNIQKNSTFTAKAVESPSWEGKKVKLPAGGAMLAVTSQTDKKKEATWDFLKYLY
                     SVESLAKWTEGTGYLPPRKDVADAQNGLKAFLAENQMMIPAIDQMDKMVSWSSFPGDA
                     GLEAEQKLLDMRDKILSGNSVNETMKATQKEINELLK"
     gene            complement(8365..8898)
                     /locus_tag="ACG8PO_002294"
     CDS             complement(8365..8898)
                     /locus_tag="ACG8PO_002294"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0207584.1"
                     /translation="MANEKATMTINCDLKEEGLEMAVFSLAQTYQIWPQIVLSGYVQL
                     SWAAIWGKHIFYNIENQFEGKKLGDITEADLIDTLQKLAYQSVQIVNIHEGWLTKLAR
                     EVGQQLGLTFSVWTVNELETIEYLARCGVYNITSRCAWAYLWKKGAQNKLFYNQNRGV
                     KRFGDGIHDHMGALFLA"
     gene            complement(8944..9087)
                     /locus_tag="ACG8PO_002295"
     CDS             complement(8944..9087)
                     /locus_tag="ACG8PO_002295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFQ0207585.1"
                     /translation="MITAHSGSDNTEENSWAFLRFCLDRQLCFEVDVRQQTDGTLILA
                     HDR"
BASE COUNT         2985 a         2041 c         1565 g         3069 t
ORIGIN      
        1 gcaggaagta ttgagttagt ttgtaaagtc tgcgatcttc aaggatcgta aatagctgat
       61 gggttgtccc atcaacacaa ataaaactca tggaaccaga aacagacttc attgatttga
      121 actcatccat acacaacacc gtcggaagga agttcatacg tgactggtaa tgcttcgttg
      181 cttcacgcaa gatccgtgtc accgtcccat ccgagacgaa gtacttctta gcgattagtt
      241 tacgtgattg attttcgtac agttccatga gaattgcctg cttcagttcc tttgttagtt
      301 gatgatgttc atcaactaag tccgttttcg cagaaaaggt ccttccacaa tcatggcaga
      361 gaaaacgggt acgttttaat tctaaaatag ttaacttttc tttgactttc aatatctgag
      421 tgcgtgtcgt ataggaaccg ttgcggataa tacgaaccga tcgacagtgt tcacaatagg
      481 aaggagagtc atccaatttt cctttaataa actgcgcttg aacgccttta tattcttttt
      541 caatcagcca ttcttcatca aaaaataaat gtttatctgt taatccgagt aattttctag
      601 tatgattatc cattgagaga ctcctttggt aggatgacgt tttggtcgac taaattttac
      661 caaaagtgag tctctttttg agttgaaacg aagaaaaagt gttagtagaa aatcgccttc
      721 gattttccac caacacaaaa tattatagaa gcaaaatcaa ttctacctta gatgttctta
      781 tatcactcta tcagtcctat ttgacaaaga gccaagaaaa agacgttggt cgtcgatgct
      841 gttctctcgc ctactcctcg tgcttgtaga agttgtggtt ctactgtggt agatggaaac
      901 gggaaagcaa ttatagtgaa aaatgggaag aaggaaacga ttgtccgttt tgaacaatac
      961 aatcatatgc ctttggttac gcgcctaaaa aagcaacgct atacctgtaa aaactgtcgt
     1021 acccattgga cggctcaaag ttattttgtc cgactcagcc attcaatcgc agatcatgtt
     1081 cgatataaaa ttgcttcttt actgactgaa aaagtatctt tgtcttttat tgcgaaaagt
     1141 tgtcaggtat ctttgactac cgttactcgt acattgaaag agtttaaaag ctatttacca
     1201 aagccttcta agaagattct ccccagagta ttgatggttg atgaatttcg ttcgcacgct
     1261 tccatagaag ataaaatgag ctttatttgc gcagatggtg aaacaggaaa cttaattgct
     1321 gtcttgccta cgcgtaaatt acctcgatta acaagctatt tcttaggctg caccaatcca
     1381 gaagaagtag aattcttagt cacagacatg aacgccgctt acttccagct taccaaacgt
     1441 gttctgccaa atgcgaaagt agtgattgat cggtttcata ttgtcaaaca catgaatcag
     1501 gcactcaatg agttgcgtat ccgtgaaatg aatgagcttc gtaaagcagg acagaaaagt
     1561 caggcagaaa aactgaaaaa gaactggcgc tttctgctaa aaaatcgtgc aagcatcaac
     1621 cattatgagt acaaaacatg gaaaagtttc cgagcaccaa aatacccatt tctcactgaa
     1681 gcaatgatga ttgatcgatt gcttgagttt tctgcgcctc taaaggaggc gtatcccttt
     1741 tttcatgaat tagtcgaagc ctttcgagac aaagaccctg acttattttt ctccttattg
     1801 gcagaacttc ccgaaacgtt agatgacggc tttcgggaga agcttcaaaa ccttctgacc
     1861 tatgaagaag gtatcaccag cgcaatgatc tatccttatt ccaatggaaa aattgaagcg
     1921 aagaataccc acataaagac aatgaaacga gtatcctacg gatttaaatc attcgagaac
     1981 atgagaatta gaatcttttt gatcaatcaa ttaatcaatg taagataaca aaaaatctga
     2041 accagaatgt ggttcattct gacccggatt tgattttgca caactcatca gtccttattg
     2101 acaaagagcc aaaaatctga gccagaatga ggttcattct gacccagatt tgattttgta
     2161 caacccatca gtccttattg acaaagagcc cttcttttta gtattattat aattcttcca
     2221 tgcgttcttc cagtcgctgt aaatcgcttg gacttaattc cccaattttt ttccatttga
     2281 cctttttgtt gtttttgggt aattcgttta gagttcctac gtcaatccaa gattcttttt
     2341 ttagtcctgc ctgtttccaa tcccttattt caacgtattt cagctttatt ttatcggatt
     2401 tagtatgata ttttgaagta attgacagaa acgaaattgt gtccagtcca ttttttagaa
     2461 tatagacagg ccggcgttta ccgccctctg cagctgtgaa agcaacgtag acggttgcta
     2521 tatcattagt attcatcttc attgaaccat tcttcaattt tttcgtcagt gtctagtttt
     2581 tggacaggca atttttccgc taatctttga atattatgga ttcgtttact tttttccgac
     2641 aattcaatag aaaatgggat ttttccttgc tcaacaattt gcttataaaa aacggtgacg
     2701 gctgttttgg ggctcatgcc tagttcatca ataatgcttt ctgcttcatt tttcaatttt
     2761 tcgtccatag aaatttgtaa cttagccatt ttctccacct ctttctgaca aacacattat
     2821 atcacggaaa gagttcaaat ggaattaata caactacttt tgaactcctt gaaatgcagt
     2881 gtatttatct aaatataagg aaaggatata gtgctaaatg gccttgatag atagaaaagt
     2941 ttcaaaatcc gccacagcga taattgaagc gaacactcaa cgacacacag aacaggaaaa
     3001 gactcctaaa agaaccaaat taactaggac gtgtctgaaa actccacaaa gatgtggtat
     3061 gctctggtaa aaaacgaatg gcacaaccac gaagaccgat atggttcttg gaaaactttc
     3121 tccacccttt ttgtaattgg agggatagtg gtttattgat agcgattttc caagttcttc
     3181 aagtggaacc agacattgag caccacggct aaaaagtgaa tgaagtcttt atttttaatc
     3241 aataattttt aatgattacc agtaattggt tttttcatgc gccctatttc tatttgatgt
     3301 ggagttaaat aatgctgata agtaaaaatt tttccgtcaa ttaaactgcc actagtgaat
     3361 cctgttctat tggtaaaaag agaatagtct gggtattcat ctacaccaaa tgataaagat
     3421 tcagccataa actgtttttt accagcaaat tcagcactgc taccttgatg gatatgtcct
     3481 acaaaaatac ctaaaatatc actttcggca atcaattcag caaaaccttc cggaacttca
     3541 gttacaatag atggatcttc ccacgacaat ggatgatgaa ccaataaaat cgttcccttt
     3601 tcagcaggtg tacccaacca ctcttgtaat tgaatcatct gtcctatcga tatttttcct
     3661 tcgtgctggg tatcttgagc agaatccaag gaaataattc gtaacccatt ttgattcgta
     3721 caatggatat actctcccgt ttcattgctt tcaacactca ttccttcaaa gaaaacactg
     3781 cgacgatcat gattcccacg acaaaaatag taaggtgtgg ttgggaaata ttgatccaac
     3841 attttccgta agacttgata atcagaaatc cccccttcat gtactaaatc acctgttaat
     3901 agaataaaat cgtacactgt taacgagagt tcttgtaaca atttttccaa tgccaactgt
     3961 ggatttttaa ttgttggaaa tagttcagca gtaaacaagt tagtttgata gttttctcta
     4021 aaatggatat cagagagatg caaaaagcgc atggctattc cctctttctt caataaaata
     4081 atcaggatat cctaaatttt cttgcgtctg ataatcaaaa aaatgcattc tttcaggttc
     4141 tatgcggaga ttgactgcat caccaatctg ccaagtaggt ttttcattaa ggacaatcat
     4201 ttctatacca tcgactttaa cggtgtacgc ataactttga cctaatagct cacgatactt
     4261 cacttgaccg tgtaaatcgc ttgtttcttt tgaaaaggtt acgtgttctg gtcttatgcc
     4321 taaatacaat gagccactaa cctgaagaag ttgtttttgc caagctggat tcaatgttaa
     4381 aaattgactg gctaccttta atgttccttg atcataagta actggcataa tattcatgcc
     4441 tggcgaacca ataaaatttg cagtaaaagt atttttaggt ttcgtataaa catttttcgg
     4501 cgtatcaatc atctgaattc taccttcatt caataaaaca atccggtcag ctaacgtcat
     4561 cgcttcaatt tgatcatgag tgacgtagac aaaagtttgt ttataacgtt gatgaatttt
     4621 taccaattct tttcgtgcat ccaatcgtaa acgcacatcc aaatttgata acggttcatc
     4681 taataaaaaa taattactcc gtttaaccac cgcacgagcc aatgctacac gctgccgttg
     4741 ccctcctgac aaatctttag gaagtcgatc ttggtatggc gttaaaccta acatcttcaa
     4801 agcttcttcc aaacggtgct gaatgtcagc tgtcttaatt ttattcgccc ttaatgcaaa
     4861 aataatattc tcctttacag tcatgtgagg atataaagca taattttgaa aaaccattgc
     4921 gatctctcgt tctccacaag gtagcttatt tgctagttgt gtacctaaaa aaagctctcc
     4981 actcgtaatt ttttccaaac cagcaatcat tctcagcaaa gtagattttc ctgaaccaga
     5041 tggtcccagt aacacaattc gttctccagc ggccacagct aaatcaattt cttttaaaat
     5101 ggtatgttct ccatattttt ttccgatatt tcttaattca agcgtttctc ccatgatttg
     5161 tcctccaatt tatttaatac ctgcagaaac aaaagtcccg ataatatatt tttgaaaaat
     5221 tgcatacaag actaacggta caaacattgt aattaaacta atcgccattg ctacggtaaa
     5281 atttgtccca ccttcagaac taatataggt ctgcatcgcc aacggcaaag tataattatc
     5341 tactgtccgt aaaattaata ccggccaaac atactcattc caagaggtaa taaaaaacca
     5401 aataccggta gaagtaatcg catgtctagt aagaggaaaa acaattttac tcatgacggt
     5461 tgtttgtttg atgttatcca atgccgccac atctagaagg ctcactggaa tttcccgcat
     5521 tgtttgcgtc aataaaaaaa tgcccatcgc atcagcaaat tgtggtatta tcacacccca
     5581 agtagtatca attaaattaa gttttgcaat caacaaataa ttaggaatca tcgcaacagt
     5641 aaaaggaatg aacatcgtac caatgatgat aaagtaccag attttcttcc ctttgattcg
     5701 gcaataagta aaactatatg ccgcaaaaaa agctacgcct aacttaacaa ccgttataat
     5761 tgtagcaata aaaaaagtat ttctcgtaat tttgataatt ggaagatttt cgaacaaata
     5821 gcaaaaattt tccaaggtaa acggctgtgg tatcaaacta gtaatcgtct gataggcatc
     5881 tttcaatggc ttaaaagaat tggccataat aaacaataat ggatagataa taatgattat
     5941 gatgaacata aataagagat gtggcaacca gcgtttaatt ttcatagtaa accccctttt
     6001 ccacaaattt aaatattacc cctaagaaaa cagcaaatat taataaagtt aaaactgaaa
     6061 ttgctgaaga ttctccgact ttatacattt caaaagcctt ttgataagca taatagatca
     6121 cgtttgcact accattattc ggtccaccac gagtgatgat cttaatcgga gtaaacacat
     6181 actgcagacc ttgaacaatg gtcatcacca ataaatatag tcctgtcgaa ctactcatag
     6241 gcaacacaat tgtaaaaaaa atgcgccaga tgggtacacg atccaactta gctgcttcga
     6301 tcatctcttg atcaattccc acaattgctg caaaaatgac aatgaaatta taaccaaaaa
     6361 ctttccaagc agtaatacct gcaatcacag caattaccca accttgtaaa ttcatccaca
     6421 taggcagtct taaaccaaaa tttcccaaca agacagcaat tggtcccgat accggattta
     6481 atatccatgt aaaaaccatc cccgccacta ccaaagaaat cacagctgga ataaaaaata
     6541 atggtttata caaattctgc cctttttcta taactattcc taataaaaag gatgtaatat
     6601 atgggaacac acagctcagc aagacaaata gaacggtata ggacaaagta ttcttcaaca
     6661 ctttatgaaa gactggatca gtcagcacct ttacatagtt ggcaaagcca acaaattctt
     6721 tagtgggggc caccatattc cagtcaaaaa aactcaaata gatcgtataa atcaatggat
     6781 aaaaaacaaa cacgcccaac aaaataattg ctggcgccag atacaaataa cctacatagc
     6841 gcttcatttc cgacagcctc ctcatcctta tgtcaaaagc agacgacctt gtttttcctt
     6901 tcttacaggc gctgtcgtct gcttgctttg cttatttgag taactcatta atttcctttt
     6961 gagtagcttt catcgtttca ttgacggaat tacctgataa aattttatcc cgcatatcca
     7021 acaatttttg ttcagcttct aatccagcat ctccaggaaa tgaactccaa ctgaccatct
     7081 tatccatctg atcaattgct ggtatcatca tctgattttc agctaagaaa gcttttaatc
     7141 cattttgggc gtctgcaaca tctttacgtg gtggtaaata accagtacct tctgtccatt
     7201 tagctaaaga ttcaacacta taaagatatt ttaagaaatc ccaagttgct tcctttttct
     7261 tatccgtttg tgatgtaaca gctaacattg cgccaccagc aggtagttta acttttttcc
     7321 cttcccaact tggtgactca actgctttag cagtaaaagt actatttttt tgaatgttgt
     7381 ccctttgagc aatagtggta tagaccatcg cgacgttacc atcaataaag ctttgcacac
     7441 cttgttccca cgtagtgtgt aatgccgtct ggtcttttac catcctttga tataattcat
     7501 aagcagcgat accatcttta gaagcaaagg tagctttgcc atcttttatt acacttgccc
     7561 cattactttc taaaattgct tgttgtgccc aattatcagc aggttcttga atatacacgc
     7621 catactttcc agttttttct tttattatct tagaaaaagt ttgaacttct tcccaagttt
     7681 ttggaccatt ttcatccaag cccgctgctt ttaatatatc ttgatttaaa tataaaaccg
     7741 gattactaat ggagtaagga atacccactt gtttaccatc tttttcagac agtgccatga
     7801 tattgtctaa aaaatgatct ttaaaataat cagcattatc cggatccact tcttctacta
     7861 aagattgagg attagtatat ttgaaatttt ctgcaaaata atctttaaaa gcccatccca
     7921 cttgtactac gtccggtatt tctttggagg caacagaagc ttgtaaattt tgcatcaagc
     7981 ctttatacat atcaggatta tattttgcta caaccttcac tttgtcttga gattgattgt
     8041 atttatctac taattcattc actgtttttc cacctagggt atccgcatta acatgccaat
     8101 actctatcgt tacacgaccc tccgattttt ttgttgtctt ggtattccca ctaccacaag
     8161 ctgccaaaga gatgacactt aaaacagaca caacccctat aacaagattt ttctttaaca
     8221 taacattaac ttcctcctaa tttctttatg gccatttttc taatcaagaa atattatgaa
     8281 ctaccaatgt aaatttctag ttaattttct gtaaataatt cgaaatttgt gcatttcggg
     8341 gtacagggtc actggacata tccatcaagc taagaataaa gcccccatat ggtcatgtat
     8401 cccatctcca aatctcttaa ctcctctgtt ttgattataa aatagtttat tctgtgctcc
     8461 tttcttccat aaataggccc atgcacaacg tgaggttata ttataaacac cacagcgcgc
     8521 aaggtactca atcgtttcta gttcattgac tgtccataca gaaaatgtca atcctagctg
     8581 ctgccccact tcacgggcca acttagttaa ccatccttca tgaatattga cgatttgaac
     8641 gctttgatag gctaatttct gtaaggtatc gatcaaatct gcttcggtaa tatccccaag
     8701 ttttttccct tcgaactgat tttcaatatt ataaaatata tgctttcccc aaatcgcagc
     8761 ccaagataat tgtacatagc cactaagaac aatttgtggc cagatttgat aggtttgtgc
     8821 cagcgaaaaa acggccattt ccaatccctc ttcttttaaa tcacagttaa tggtcatggt
     8881 tgccttctca ttggccactg cttgaaacac ctgctcaagg gttaccgaat ctgggacaac
     8941 ttcttacctg tcatgggcta gtattagcgt accatctgtt tgctgacgca catctacttc
     9001 aaaacaaagc tggcgatcca aacaaaagcg aagaaatgcc cagctattct cctccgtatt
     9061 gtcactacca gagtgtgcag tgatcatggt cattttgacc cctcctttaa ttccgtgatt
     9121 ttctcccaca cgtccactat accgatgaag cgttaatttc ttgttaattt cttgtaaatt
     9181 ccctgcgttt tttcagtaaa ctagttgtaa atcttgcatg ctccttccta cgaactcatg
     9241 aatcgtcgat tttgcccttc tagctagttt caccaattaa gtagttctac attccccgct
     9301 tttaacctac tacacctttt tttgttttca gctattcctt cttcttgctg aaagtccttg
     9361 aggttctttc ttacaggata ccatcaaggt gttcaatggc ttattcccat tgaatgaccg
     9421 agacttacta tcagatttct gctcgttgaa ccaagtcttt gatacaggat ttcttccgtc
     9481 atcttctcac aaataccacc ttgcgtttct ctagcgattg gtcgatgtgc accctcccag
     9541 tggactgtca ctaccccttt ttgctatgcc tagcccacaa aaaaagctgt cctcacagac
     9601 agccagactg tagacaaaag ctgttccaaa ctcgtaactc gagcttggaa cagcttttta
//
LOCUS       DAZKKW010000092         9462 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.094, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000092 DAZKKW010000000
VERSION     DAZKKW010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9462
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.094"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..135
                     /locus_tag="ACG8PO_002296"
     CDS             <1..135
                     /locus_tag="ACG8PO_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFQ0207586.1"
                     /translation="FNQVNQGFISSVASKRNHIPRKSLNYQTPLEVFLSYVNGKFCLA
                     "
     gene            complement(88..300)
                     /locus_tag="ACG8PO_002297"
     CDS             complement(88..300)
                     /locus_tag="ACG8PO_002297"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0207587.1"
                     /translation="MENHVLETGMQKITTHASITAKPFFEKRGYKVINEQTVELRGQL
                     FTNFLMILFVKLSETKLSIYVTQKNF"
     gene            complement(744..1865)
                     /locus_tag="ACG8PO_002298"
     CDS             complement(744..1865)
                     /locus_tag="ACG8PO_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HFQ0207588.1"
                     /translation="MDKYNVKTSFQKWFSAINFSGLSENSQSLISDFDFYSKKLDFQT
                     TLKVLLHAVYEELPSYREIDRAFLDQRLCKELGIDSLCYSSLSRRAPEIKQEVLMEIF
                     TQLVARISAQQPSSKTTSLQLIDSTTIPLNKALFPWAKFRKTKSGIKLHLNLCYLDKT
                     NQYPESFTMTNASEHDRNHLEVLVDKTQATYVVDRGYFDYKLLDKLNRDGYFFVTRTK
                     SNTKITILDQIEVADTTTRDGTIISDQQVILVGGVNHVTERFRLVTVLTKGQKILRMV
                     TNLFDVSPNEVADMYQARWQIELLFKHLKQNLTIKRLYSHSEQGAINQVILTLIATLL
                     TYVIKIELNTTATLFQLKRTFHYLMFEPAEEWFKRYRPG"
     gene            2193..3602
                     /gene="istA"
                     /locus_tag="ACG8PO_002299"
     CDS             2193..3602
                     /gene="istA"
                     /locus_tag="ACG8PO_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HFQ0207589.1"
                     /translation="MDDIKTIKHLRNNRDQSIDQIRKAMNINWRTAKKYADSDQLFIT
                     DPTRKNKSMMDDGWGEIIGSWLEEDLRLRRKLRRTAKAIHEQLKPLGFPGSYRTVCVY
                     ISQWKKQRYDETNYQAVDRLDHPIGEAQVDFGTMEVMHEDHMKDIQLLIMSFPYSNAT
                     SASAMPAQNQECFLEALKQLFNQCGGVPKTIRIDNLTPAVKKTKSKFEEAQLTDEFLR
                     FTIHYGCDVQVCNPRSGHEKGHVENKVGYVRYNFFSTSPIMDSFESLNKQLEEQLKAD
                     RKRTHYSKHQLIEDLWKKENKALHSLPQEDYPIFKEVEAKLNRYHEMKLDQTVIHIPK
                     AYGYSQLTVVLTWNKFKVLTPHGEILMNEYRPYMNKKRLIPWSDIMKQWSNKLNVVSY
                     SRYWKYLPARLQNYLAIDNNHLKYRRIKQLLSLLATYDIQQINEQFYELVVQEDAPSD
                     FDVNWSNYDALTKTGGVQK"
     gene            3599..4327
                     /gene="istB"
                     /locus_tag="ACG8PO_002300"
     CDS             3599..4327
                     /gene="istB"
                     /locus_tag="ACG8PO_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HFQ0207590.1"
                     /translation="MIGQTLEDYCKKLRLSHIITNIPRASFEAPEQFITDLFRLELET
                     RQQIKLERMEKLAKFPVPKSLKNYEWHEDIFLPSHLDKETLVSLDFLKRQENIVCVGS
                     PGTGKTHLAIALGRKACSEGVSTKFFRVNNLVEQLENALKKQQLASFKKRFETTELII
                     LDEMGYLPLTKEGAELLFQLISDWYEQKSIIITSNLEFSQWNRVFMDSRLTAALVDRL
                     IHHAHIISFKGESFRLKNSLSKSY"
     gene            complement(4455..5366)
                     /locus_tag="ACG8PO_002301"
     CDS             complement(4455..5366)
                     /locus_tag="ACG8PO_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFQ0207591.1"
                     /translation="MKIKIERSNKEKIPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GIYKNFTAIDQHTTHEKVVVKEKIVNTSGVENFTVDFVKEYFSWKNNKEVIEKRMTNL
                     EQYLTEEGLALSQDMVRADIPTSSEVVSVKILDVEKHSEEFVVSFVVDQKITEGKKTQ
                     SISSAYRLMIFEDEKGNHIVTSLPTMIGKPEKAKHKVRQVKSDSKIDSKTTEEITEFL
                     ETFFRLYPAASEKELEHYVEKDAMQPINTNLKFVELVNPIYKQKGKAIQVIVGVEYLD
                     ELSKTTSVFQYRLKLYKNENWTISNCE"
     gene            complement(5383..6390)
                     /locus_tag="ACG8PO_002302"
     CDS             complement(5383..6390)
                     /locus_tag="ACG8PO_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HFQ0207592.1"
                     /translation="MNLKKLSIISLTFLTISFVGILMCVGLLFGEDGEGSGSSGTSPG
                     GSSVSEVVLKHRSMVEKYAKESDITEYVSILLAIIQVESGGTMEDVMQSSESAGLSPN
                     SLSTEASIKQVCLYFSSLVKCSKELGCDQDSIIQAYNYGDGYLDYVAKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENSGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSSPGTGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLIFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTDSYWQQHIIGAGRYK
                     Q"
     gene            complement(6387..8516)
                     /locus_tag="ACG8PO_002303"
     CDS             complement(6387..8516)
                     /locus_tag="ACG8PO_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFQ0207593.1"
                     /translation="MKKKLFRIVGIVFISIVTLTVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLSAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAKSIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRTVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALSLGTKIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEGRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKRAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRTFAFGGGAAVGGKLATSSTKGKGSSKINRVSNRKNHSARSINELNSDLKK
                     QEQPVGTKKESRINLVGRKVGKVLDTKEIVKDKAKQAKNQVTDAPTNLKYSLHKGMEK
                     TKKVPEDFKRGPFEEKANRGELREKQRQRRDAKMDEKRKVLNEAWNRHEKGRENVPIK
                     KEIKPQKNELPENRSLKPRVQVHSNPEIKRKLSSQEVIPNGSTQPLSKNNSFQQVKQR
                     FTFPKRTNQQVQRKKNTRPKPKPGEKR"
     gene            complement(8521..>9462)
                     /locus_tag="ACG8PO_002304"
     CDS             complement(8521..>9462)
                     /locus_tag="ACG8PO_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFQ0207594.1"
                     /translation="RGGWKETLPEIADEISILNLTSKSENQGLLDPYVIMKKKKDSES
                     LAIDILTFLTGISSRDGEKFPVLRKAIRTVTQSDQRGLLQVINELRKDGSPIAENIAD
                     HIESFTDYDFAHLLFSDGSITQSISLDKQLNIIQVADLVLPDAETTFEEYTTMELLSV
                     SMLIVISTFALDFIHRNREIFKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYF
                     VTQNSDDLLDERLKNNIGLKFAFRSTDIHEIKKTLEFLGLDQEDQSNQKRLRDLENGQ
                     CLMQDLYGRVGVVQVHPVFEELFHAFDTRPPVQERME"
BASE COUNT         2896 a         1943 c         1649 g         2974 t
ORIGIN      
        1 ttcaaccaag tcaatcaagg attcatctca tccgttgcgt ccaaaagaaa ccatatccct
       61 agaaaatcac taaattacca aacaccatta gaagtttttt tgagttacgt aaatggaaag
      121 ttttgtctcg cttaatttga caaataatat cattaaaaag ttagtaaata attgccctct
      181 aagttccacg gtctgttcat taataacttt atatcctcgt ttttcaaaaa atggctttgc
      241 ggtaatggaa gcgtgggtag tgattttttg cataccagtt tctaaaacat ggttttccaa
      301 ttcatctgca attttagtcg caataccttt tccttgatgg ctatgttttt gtcacaactt
      361 cagtattcct gtaacaatac ggatttcagt atacaacaac agcttttgaa aagatacttg
      421 caaaaggatt aagatatcta tgacctgcta tgctcactga tgttgaaaag aatgttttat
      481 taggattgag gtaagcaaga tagtgaatat gtccggaaag cctgtaaaat aaggcttttc
      541 ggacatatgt gaagttataa aaagatagcg aaaaatctat ttagtatcac taatgttgcg
      601 aacatatcgt cagaatagtc tgacatttat ttcttgctaa aaatttcaaa aaagtcaagt
      661 gctgacgcag cggatgacta ttttccagaa aattttagta tgtattcaaa aatcaaacgt
      721 gacaacagct attaactaca tcctcatcca ggtctgtaac gtttgaacca ctcttctgcg
      781 ggctcaaaca ttaagtaatg gaaggtacgt ttcaattgaa acagcgtcgc tgtcgtattc
      841 aactcaattt ttatcacata agtgagaagc gttgcgatta aggtgaggat gacttgattt
      901 atcgcccctt gttcactatg tgaatatagt cgtttaatgg tcagattttg ttttaagtgt
      961 ttgaacaaca attcaatctg ccaacgggct tgatacatgt cggccacttc gtttggcgat
     1021 acatcaaaga gattggtcac cattcgcagg attttttgtc ccttggtaag aaccgttacc
     1081 aacctaaaac gctctgtcac atggttgaca ccgccgacaa gaatcacttg ttggtcactg
     1141 attatcgtcc catcacgggt tgttgtatct gctacttcaa tttgatccaa gatggtaatt
     1201 tttgtattag attttgtccg tgtaacgaag aaatagccgt cacgatttag tttgtctaac
     1261 aacttgtaat caaagtagcc gcgatccacc acatacgttg cttgcgtttt atcaacaaga
     1321 acttcaaggt gattacggtc atgctcactc gcatttgtca tcgtaaaact ttcaggatac
     1381 tgattggttt tatcaagata acagagattc aaatgtagct tgatgccaga ctttgttttc
     1441 cgaaacttgg cccatggaaa caatgctttg ttcaacggaa tagtcgttga atcaatcaat
     1501 tgtagactgg ttgtttttga gcttggctgc tgagcagaaa ttctggcgac taattgcgta
     1561 aaaatttcca tcaatacttc ttgcttgatt tctggcgctc tacgagataa cgagctgtaa
     1621 cacagggaat caatgccaag ttccttgcag aggcgctgat ctaaaaaggc gcgatcaatc
     1681 tcgcgatagc ttggcaattc ttcataaacc gcatgaagta acactttaag cgtcgtttgg
     1741 aagtctagtt ttttactgta gaaatcaaag tcgctaatta agctttgaga gttttctgat
     1801 aaaccagaaa aatttattgc agaaaaccat ttttgaaatg aggtttttac gttatactta
     1861 tccatgcgtg agtccttttt attggtcttg gataagtcat ccgagaatag tataaaggac
     1921 ttttttgcgt ttggatagct ttgtattagg cttttgatgg gttaaaaagt ctttgcaaca
     1981 ctagtaattt agtatttatg tataaattga aagatatttt accaccactt agtcttctta
     2041 aaagtgatcc aacattttgt ttgtggcaga atttaactac acactaatgc agccgaaagt
     2101 acacaactat gcagcagttt gactgcgaac tggtttcctt ccatcataag tttgaatgac
     2161 ttagtgatgg aggtacagaa aggttgttaa ctttggatga tattaaaaca ataaaacatt
     2221 tgagaaataa tcgtgatcag tcaattgatc agataagaaa agcaatgaat atcaattggc
     2281 gaacagcgaa aaaatacgcc gatagtgatc aactattcat tacagatccc actcgaaaaa
     2341 acaaatccat gatggatgat ggttggggag aaatcatcgg tagttggcta gaagaagacc
     2401 tacgtttacg cagaaaactt cgtagaacgg ccaaagctat ccatgaacaa ttgaaacctt
     2461 tgggtttccc tggctcctat cgaacagttt gtgtatacat ttctcaatgg aaaaagcagc
     2521 gttacgacga aacgaattac caagctgtcg atcgtcttga tcatccgatc ggagaagcac
     2581 aggtcgattt tggaacaatg gaagttatgc atgaagatca catgaaagat atacaacttc
     2641 ttatcatgtc atttccctat agtaatgcga cgtccgcaag tgccatgcca gctcaaaacc
     2701 aagagtgttt cctggaagca ttaaaacaac tatttaatca atgcggcggc gttccaaaaa
     2761 cgatcagaat agataactta actccagcgg tgaaaaaaac aaaatcaaaa tttgaagaag
     2821 cacaactaac cgatgaattc ttacgtttca ccattcatta tggttgtgat gttcaagtct
     2881 gtaacccacg tagtgggcat gaaaaaggac atgtcgaaaa taaagtgggt tatgtccgat
     2941 acaatttctt ctcgacttcg ccaatcatgg actcttttga gtccctaaat aagcaacttg
     3001 aagaacaatt aaaagcagat cgaaaaagga cgcattattc aaaacatcaa ttaatcgaag
     3061 atttgtggaa gaaagaaaat aaagcgttac attctcttcc acaagaggat tacccaattt
     3121 ttaaagaagt ggaagctaaa ctaaatcgtt atcacgaaat gaaattggat caaacggtta
     3181 ttcatattcc taaagcatat ggctattctc aattgactgt tgttttgact tggaataaat
     3241 tcaaagttct tacgcctcat ggagaaatat taatgaacga atatcgcccc tatatgaaca
     3301 aaaaaagatt gatcccatgg agtgacatca tgaaacaatg gagtaataaa ttgaatgtcg
     3361 ttagttattc gaggtattgg aagtacttgc ctgcacgcct tcaaaattat ctagcgattg
     3421 ataataatca cctgaaatac agacggataa aacaattact aagtttatta gcgacctacg
     3481 atatccaaca aattaatgaa caattttacg aattagtcgt tcaagaagac gccccctctg
     3541 actttgatgt gaattggtca aactatgatg cattaacaaa aacaggaggt gtgcaaaaat
     3601 gattggacag acgttagaag attattgtaa aaagcttcga ttaagccata tcatcacgaa
     3661 cataccaaga gcttctttcg aagctccaga acaatttatt accgatttat tccgtttaga
     3721 actggagacc agacagcaga tcaaattaga acggatggaa aaattagcca aatttcctgt
     3781 accaaaatcg ctaaagaact atgaatggca cgaagatatt tttctaccat ctcatctaga
     3841 taaggagacc cttgtctctc ttgattttct aaaaaggcaa gaaaacattg tttgtgttgg
     3901 ctctcctggt acagggaaaa cacatctagc gatcgcatta gggagaaaag cctgtagtga
     3961 aggtgtttca accaaatttt tccgagttaa caatctcgta gagcaattag agaatgcatt
     4021 aaaaaaacaa cagctagcca gtttcaagaa gcgattcgaa acaacagaat taattattct
     4081 tgatgaaatg gggtacttac cattgacaaa agaaggagct gaacttctct tccaactgat
     4141 ttcagattgg tatgagcaaa agagcattat tattacctct aatttagaat tcagtcaatg
     4201 gaatcgagtg tttatggatt ctcgattaac agctgctctc gttgatcggc taatccatca
     4261 cgctcacatt atcagcttca aaggcgaaag tttcagattg aaaaattcat tatctaaatc
     4321 atattgaagg aaaaaactag ttgcagcaaa actctgcact ttcggctgca tttttttgta
     4381 aaaagctatt gcacaacaca aacatttgaa cgacgtaatt ccaaatgttg gattgctttt
     4441 agataatctt ttatttattc acaattgcta atagtccaat tttcattttt gtatagtttt
     4501 aatctatact gaaaaacact tgttgttttg gacaactcat caagatactc aacaccaaca
     4561 atcacttgaa tcgctttacc cttttgtttg taaataggat tcactaattc cacaaatttc
     4621 aaattagtat taattggttg catcgcgtct ttttcaacat aatgctctag ttctttttct
     4681 gaggctgctg gataaagcct gaaaaacgtc tctaaaaact cggtgatttc ttccgttgtc
     4741 tttgaatcaa tttttgaatc actttttact tgtcttactt tatgttttgc cttttctggc
     4801 ttaccaatca tagtgggtaa actcgttaca atatgattgc ctttttcatc ttcaaagatc
     4861 atcagacgat atgcagaaga aattgattgt gtctttttcc cttctgtaat cttttgatct
     4921 accacaaatg aaacgacaaa ttcttccgag tgtttttcaa catctagaat ttttactgat
     4981 acaacctcgg aactagtggg aatatccgct cgaaccatat cttggctcaa agcgagccct
     5041 tcttctgtaa ggtactgctc aagattagtc attctctttt caattacctc cttgttattc
     5101 ttccatgaaa aatattcttt cacaaaatct acagtaaaat tttctactcc agaggtattc
     5161 actattttct ctttcacgac cactttctca tgagttgtat gctgatcaat ggccgtgaag
     5221 tttttgtaga ttccaaaagc aaggctacaa gacaataaaa gccaaagggc aagcaccatt
     5281 tttcgatgct tgcccacgga tactactcgt tctttcttct tttttggaat cttttccttg
     5341 ttacttcgct caatttttat cttcattttc cattcctctt ttctattgtt tataccgacc
     5401 agcaccaata atgtgctgtt gccaatagct atcggtaaga ttggtccaac ccaccggatc
     5461 tcctgcatga aacattctgt tctctcctgc ataaatgccc acatgagtca cataagtacc
     5521 ggcatcataa gttgaatgaa agaaaatcaa atcgcctggt ttggcatctt ttaagtcaat
     5581 atgagtcata gcatcatatt gatcctgagc cacccgaggg agtttcaaac caactttggc
     5641 ataacaccat tgagttaaac cactacaatc aaacccagtt ccgggacttg acccaccaaa
     5701 tacataagcc gttccttcat acttgaacgc ctcgtcaaaa attccttgga ctgttttatc
     5761 atcaaactgt gttgtagtta ggtactgctt cactaataag caataaaaca tattgccata
     5821 gttataccgc cacccactat tttccttaat cgcgatggga ttgggataat cgactttttg
     5881 accacccgat ttttcttttg aaaaagattc ggctaaagca aaactatgct tctttccgtt
     5941 tttcgcgaca taatccaaat acccgtcacc atagttataa gcctgaatga tactatcttg
     6001 atcgcatcct agttctttgg aacacttaac caaagacgaa aaatagagac acacttgttt
     6061 gatggatgcc tcggtactta atgaattcgg tgataaacct gctgattcag aactttgcat
     6121 gacatcttcc attgtacctc cagattcgac ttgaataatc gccaaaagaa tcgaaacata
     6181 ctctgttata tctgattctt tggcatactt ttccaccatt gaacggtgtt tcaaaactac
     6241 ttctgagaca ctactacctc caggagaagt tccagaacta ccacttccct caccgtcctc
     6301 accaaacaac aaacccacac acatcaagat gcccacaaaa gagatggtca ggaaggttaa
     6361 cgaaataata ctcaattttt ttaagttcat cttttctcac ctggctttgg ttttgggcga
     6421 gtgttctttt ttctttgtac ttgttgattg gttcgctttg gaaaagtgaa tctctgtttc
     6481 acttgttgaa aggaattgtt cttgcttagt ggctgagtgc ttccgtttgg aatgacttct
     6541 tgactagaaa gtttccgttt gatttcagga ttcgaatgta cttgtaccct tggttttaaa
     6601 gacctatttt ctggtagttc atttttctgt ggttttatct ccttttttat cggaacattt
     6661 tcccttcctt tctcatgacg attccatgct tcgttcaata ctttgcgttt ttcatccatt
     6721 tttgcgtctc ttcgttgtcg ttgtttttct cgcaactccc ctcgatttgc tttttcttcg
     6781 aaaggccctc gcttaaagtc ctctggtact tttttcgtct tttccatgcc tttatgaagg
     6841 ctgtatttca agttagttgg tgcatcggtt acttgatttt ttgcttgttt cgctttgtcc
     6901 ttcacgattt cttttgtatc gagtactttt ccaacttttc taccaactaa atttattcgt
     6961 gattctttct tcgtgcccac tggttgttct tgttttttca gatcagaatt tagctcgtta
     7021 atgcttctcg cggaatggtt ctttctattg gacactctat tgatttttga actacctttc
     7081 ccttttgtag aactggtagc tagtttccca ccaactgctg caccgccacc aaaagcaaaa
     7141 gttcgaccca catttcgatt aagctgtcgc aattttcgat tcatcaacat ttgcggtttc
     7201 cgcatgactc gccttcccat tgactgacta tcattacttt gaaggttaaa catgctcata
     7261 atgtcaccta atttgaaata gataccggca aaggtcacaa tttgtagaaa agctaccata
     7321 aagaagggat aggtggccga aatattgaaa aacatcgtgg agatactgaa tgcagtcgta
     7381 atgactaagg taactcccgc tcgcatcata atcgtattga acaaacgaat aatcgccctt
     7441 ttgcccaaac tctcatacgt cggcaacatc gacaataaga aactaatggg cagaaacatc
     7501 gcaaagataa tgaaaaggat ttgtgagaac aacataatcc ctgtcagaag aaacacaaaa
     7561 aaggaaatcc caatattaaa caacactaaa aagaccacgg ttcctaaacg gttcatggtc
     7621 ttggtaatac tgagattcgc attgtcatta tcttcaatct ctgctttgac tgcttcttcc
     7681 cgatccttac ccttattttc atccggactc acggataaaa tcttattaac ccgaccctcg
     7741 ccaatttctt ctatattgga atcatcgaat tgtaatagta accagggttg ttgaacttga
     7801 atcgaaaata aactgtctcg aatcaaatcc acactatctt ttccctggct ttctgaatta
     7861 ggtacaacaa tcttggttcc aaggctaaga gccgcttcac taacgtctga gctaaagtca
     7921 ttgatttttg taatataggt gggcgcatac gcaatgaaag aacccgagag tagaaaaatc
     7981 atcacaaaat taatgacggt tcggactgct ttggtagttt ctcgttttaa taatccggta
     8041 tacgccacat aaactcctag agctaaaatc atcaataata ggaacccaaa gtagaatccc
     8101 gaagtctgta atccattttc agtgataccg gctaaagttt ggatattctt tccaatactt
     8161 ttagccgtat ccgaaatgaa atccaacttg taagcttctt gaaccacata gcccgtggcg
     8221 tttgataaat acagacttac tgtccaaata aaattggtaa tggcagaaag accatacatc
     8281 acgcttttcc ctaagccatc cccccaattc caaggtaacc aatcccaaga actatccaca
     8341 aagaaatcca gttgataatt gttcagagaa tatttggaat aaaggtttcc ggcttcaact
     8401 gtatcatcaa ccagacctgt cgcctcagct accgtaccga ctaaactcaa tagaactgtc
     8461 agagtgacga tgctaataaa aacgattccc acaatccgaa aaagtttctt tttcatcccc
     8521 tcactccatt ctctcttgaa ccggcggacg agtgtcaaaa gcatggaaga gttcttcaaa
     8581 gactggatga acttgaacca cacctactcg cccatacaag tcttgcatca agcattgacc
     8641 gttttccaaa tctcgcaggc gtttttgatt gctttgatcc tcctgatcca aaccaaggaa
     8701 ttccaaggtt ttcttgattt catgaatatc ggtactgcga aaagcaaact tcaacccgat
     8761 attatttttt aaacgttcat ctaataaatc gtcgctattt tgagtgacga aatagacccc
     8821 agcattcatg gcccgaccag cacgaacaag cttattcgat aacgtttttc cttgagccac
     8881 ttgaagaaaa ctccaagcct catccaaatc gacaattttg aagatttctc ggttacgatg
     8941 gataaagtcc aaggcaaaag tggaaatgac gattagcatc gaaacactaa gcaattccat
     9001 ggtggtatat tcttcaaaag tcgtttctgc atccggtaaa accaaatccg ccacttgaat
     9061 gatatttagc tgtttatcta aactaatcga ttgggtaatc gagccgtctg agaaaagcaa
     9121 atgagcaaaa tcataatcgg taaaggactc gatatgatcg gcgatatttt ccgcgattgg
     9181 tgacccgtct tttcgcaatt cgttaataac ttgtaataac ccccgttgat cactttgagt
     9241 caccgttcga atggctttac gtaacacagg aaacttctcc ccgtctcgac ttgaaattcc
     9301 tgttaaaaaa gtcagaatat caattgctaa actttccgag tctttctttt tcttcataat
     9361 cacataagga tcaagcagac cttgattttc tgacttactc gtcaagttga ggatactgat
     9421 ttcatccgca atttctggca aggtttcttt ccaccctcct cg
//
LOCUS       DAZKKW010000093         9444 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.095, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000093 DAZKKW010000000
VERSION     DAZKKW010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9444
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.095"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            59..205
                     /locus_tag="ACG8PO_002305"
                     /pseudo
     CDS             59..205
                     /locus_tag="ACG8PO_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
     gene            282..611
                     /locus_tag="ACG8PO_002306"
     CDS             282..611
                     /locus_tag="ACG8PO_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFQ0207595.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            601..888
                     /locus_tag="ACG8PO_002307"
     CDS             601..888
                     /locus_tag="ACG8PO_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFQ0207596.1"
                     /translation="MAYEIVPSKHVIKYLKKLKEKPLKEKFLNLIYDEIAIDPYKGEQ
                     KTGDLSGIWSSGFKYAGTTYRVAYEIIENKVIPVLLCGTHENFYEQLKKLI"
     gene            1174..2031
                     /locus_tag="ACG8PO_002308"
     CDS             1174..2031
                     /locus_tag="ACG8PO_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFQ0207597.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYVEVE"
     gene            2032..2580
                     /locus_tag="ACG8PO_002309"
     CDS             2032..2580
                     /locus_tag="ACG8PO_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0207598.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            2840..3301
                     /locus_tag="ACG8PO_002310"
     CDS             2840..3301
                     /locus_tag="ACG8PO_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermophilic serine proteinase"
                     /protein_id="HFQ0207599.1"
                     /translation="MKKFKKKIYLGLVVGVSMFGFMAHTKQVTADTNQKERIMSQLDD
                     SQIEYVPNELIVSLKATQRISTEKNKPQNLLGVKVMVEKDLTLRTNESDYNKRDQVLL
                     VKLNEEISVEEAVRTFNASDMVNYAEPNYVVELEPLNDVEEPNTTDSLGNR"
     gene            3345..3431
                     /locus_tag="ACG8PO_002311"
                     /pseudo
     CDS             3345..3431
                     /locus_tag="ACG8PO_002311"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
     gene            complement(3397..4467)
                     /locus_tag="ACG8PO_002312"
     CDS             complement(3397..4467)
                     /locus_tag="ACG8PO_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFQ0207600.1"
                     /translation="MKNDIVTYANTKCEEIGISNLEECFFDTNDLSQFLRTGRTNASK
                     LANDLVAKNVLFKINTRPVLFAIKERFEEHFLIPVKDVYESISELKTDLGPNSTDSIL
                     QKMIGYDMSLSDAVEQMKTAVFYPGRGLPMMITGDTGVGKSFFVHTAYQFMKKVNVIH
                     ENAPFKVLNCAQYHNNPELLSGILFGYVKGAFTGADEDRNGLLQDADGGVLFLDEVHR
                     LPPEGQEKLFVYMDGGTYSRVGETIHHASNVRLVFATTEGKEDFLETFMRRIPIHIHI
                     PNIEERGILEKNKLSKILSLAKAKTLARLLMFLLKSLVLHKYSQNILNYYFLEKSKKN
                     RIPNFHLHFQKDLHVDNQFLNQ"
     gene            5069..6913
                     /locus_tag="ACG8PO_002313"
     CDS             5069..6913
                     /locus_tag="ACG8PO_002313"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFQ0207601.1"
                     /translation="MNYKQTAEEVLQYIGGEENISHLEHCSTRLRFTLKDNSKVNMSQ
                     LEKVDGVMGVRQNVQCQVIIGNDVVEVYDEVKALLGDNVSNTEEAPKQKQKLGSVLLD
                     FIISIFQPLIPAIAGGGVLKSLLLLASILGLISDQSQTYQILNLVGGAPLYFLPILVA
                     MTTANKLKVNQLVAVSAVGALILPEMTAMLAEGATFLSFGLDNIAYASQVFPAILTVL
                     FYAQLEKWFTKVSPKPIRIFFVPMMSLLITVPIALLLLGPLGYNVGTAFSSIIVSLYN
                     QFGWVATGVLAAVLPLMVVTGMHKAMIPYAVSSMSELGAELLYLPASLAHNISEAGAC
                     FAVSIKTKDKKLKSTAVSAGISALFGITEPALYGVTILHKRVLYAVMFSSLIGGAFAG
                     IVALQAFALVGPGIASITMFADKADPMNLVWAFAVSGISFVVAFAAALVLYKDKEVVN
                     EVETKMETAVVSDAVLSSPVNGKVISLSEVKDTVFSSGMVGDGVAIIPEEGILVAPED
                     GEITMVFETKHAIGLKTANGAELLFHIGIDTVQLEGKGFEAFVRSGEKVAKGQKLIAF
                     DIDLIKEAGFDPTVICVVTNKDNYTIRSFNLEKLVTNDSDLLAISPIA"
     gene            6969..8435
                     /locus_tag="ACG8PO_002314"
     CDS             6969..8435
                     /locus_tag="ACG8PO_002314"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016620473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFQ0207602.1"
                     /translation="MTNTTSFPKGFLWGGAIAANQVEGAWNVDGKGLSVADMATYKPN
                     LDNKDFAAHMHVTLQSIKEASEDKSDKWYPKRRGVDFYHHYKEDLALFAEMGFKTLRL
                     SIAWTRLFPTGEELEPNEKGVAFYENVFKEMQRLGIEPIVTLSHYEMPLALAVKYNGW
                     VERRVIDDFVRFANVCFDRFGKYVKYWLTFNEIDSIHRHPFTTAGIIQEKSAEGQEAQ
                     DIYQALHHQFVASALVTKDAHEKIPGSQVGCMLTKLMTYPLTCAPEDVELTLKKNLEN
                     NFYADVQVKGEYPALIEKDLENRGIQIKMEDEDREILKKHTVDFVSFSYYMSMAESAD
                     PNAERTPGNTVLGVKNPHLPSTDWGWQIDPKGLKISLIELYDRYQVPLMIVENGMGAV
                     DQVVDGKIHDPYRIDYFKAHFQQMKEAIDEGVDLIAYTSWGPIDLVSAGTSQMSKRYG
                     FIYVDADDEGNGTYNRLKKDSFNWYKEVIETNGASLFE"
     gene            8472..9314
                     /locus_tag="ACG8PO_002315"
     CDS             8472..9314
                     /locus_tag="ACG8PO_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFQ0207603.1"
                     /translation="MISIKKVLNSSVVLVEENGKEMIALGKGIGFGQKAGDEISGDKV
                     DKIFLPFEEKKSSQLLELVEEIPIEFFEITRTIVFEAEKGLDKKLNPGVYLTLTDHLH
                     FAVERAQKGVNIANRLYWEIKIYYPKEFQIGKYALTLLNEQYGIDLPKEEASNIAFHL
                     INAQSEDPDSGAGFKYAKMIGGIVNLVRYSLQKDIDTNSIHYARFITHVRFFVERYYS
                     DALLDDGEEEFYNQMWILYPSAMESAAKIKDYIEKVYHKTIPNDEVVYLGVHINRLMK
                     HANG"
BASE COUNT         3246 a         1444 c         1906 g         2848 t
ORIGIN      
        1 agcttaaatc atagataccg taagggattt tattctttat ttaaaacttt gcaacagaac
       61 ccaatcattt gagaagttaa aacctacaca tgaaggtagg tattttagaa gattgaatag
      121 acaacaccgg gttgtttata aggtagatga agatgaacac gtggttgaaa tttattcagc
      181 aaggacgcat tacgagtcac agtaattact tgtaaataca ttatctcgtg gtataatgta
      241 tttactaagt aattacatac tgttagaaat gagggattta aatgattaca aaaacaagaa
      301 aacaaggcaa ttcaatcatg ttgacagtac ctaaagattt taatgttcct aatggagtcg
      361 aagttgaagc aaagttggta gagaatggta ttctttatga atttgttgaa ccacaaaaag
      421 aattttatga ttttagcgaa gatattttgt ctgatataat tgcagaaggc tacgataaag
      481 atgagattct tgtagaattt aagaatagaa aaaacaaaat gcattcttcc tttagagata
      541 ttgctgaaga tacacttact aattctaaag taatgactaa agaggagttg gcaacagaaa
      601 ttggcttatg aaatcgttcc ttcaaaacat gtcattaagt atttaaaaaa gctaaaagag
      661 aagccattga aagaaaaatt tttgaattta atttatgatg aaattgcgat tgatccatat
      721 aagggtgaac aaaaaactgg ggatttatca ggtatttggt ctagtgggtt caaatatgcg
      781 ggcacaacat atagagtagc ttacgagatt atagaaaaca aggttattcc agtattgtta
      841 tgtggaacgc atgaaaactt ttatgaacag ttgaaaaaat taatctagtg atttgttgtc
      901 gttcttaaga aggacggctt cttttttatc gaaaatctga ttcgtctaca tgactaaagt
      961 cgcgagtatt ctctgcttct cgataaatta aataacgtca ttactttgag gaaagctata
     1021 aatcaatata tttcagcttt tccttattta tgtactacct tttacttatt aattataagg
     1081 tagtacatat atatcttttt tttggtagta actaaaaata aatatgttat aatagtatca
     1141 aatgaatatt gctaaaagaa aagaggtagg aacgtggtag cgaagaaata tgtaatcggt
     1201 aattttaaag gtggcgtagg taaaagtacg tgcgcgcaaa tgttagggtt tgaatcagca
     1261 gttaatgaag gaaaaagaac cttgataatt gacttggata tgcaaggaaa tacttctgat
     1321 gtgatgaact taacgcatat gaacttcact aacgaagaag gaggcggaga aggtgaaccg
     1381 attgtttacg aaaatactgt gactgacgtg ttgataaaca atcttccacc taaagaggct
     1441 gtctataaag taatcaataa cctctatata cttccagcag acatgtcctt tgaaatgtat
     1501 gatgattgga tcaaagacca atataatgat tcattatccc aattcaaata tatgaaagaa
     1561 aagctagaac cattatttga tgagtttgac gtcatctatt tagatgtccc accttctatt
     1621 tctgtatata gtaaatccgc gatgtacatt gcggattggg caatcgtcgt cttacagacc
     1681 caagtaaaat cgatgagaaa tgcgatgcaa tatcttgagt atatggactt ttttgtgaaa
     1741 gaatttgata caaatttata tgttgctggt gttattcctt tcatgttgga aagcggtgac
     1801 gcagtagatc aggaaatgta taaacaagcc caagagattt atggggaaca tttgattaaa
     1861 aatgttgtac tcaaaaatgc gcgcttaaaa cgatacgacg gatcaggtat tacgatggag
     1921 aagacaaaga aaggtctctt aaaacaatgg gatcgcaaaa gccacgaatt atttatcagt
     1981 attttgaaag aattggaaga acacgagaat tggtatgtgg aggtggagta attggctgac
     2041 atcaaaggac ttgatatttt tgggaacaac aaaggactcg ccaaaataaa aaaagaagca
     2101 gaagcaaaga aaaagcagca agcaattaca ccatctgaaa ataagcaatt agaagtgcac
     2161 caagttcaag aaataaaaac ttcctccaat gttgaaagac agagtagagg aagaacaaaa
     2221 aaagttggcg caccaatcag aaagtttgat aaggtttatt ccaccaagca tcctttaaaa
     2281 ctgtcgactt tgttaaatgc gacttccaga gttttagtag aaaagtatga gactaatatg
     2341 acaagagatg aattgcttag aaaagcttta gatgagtata ttaaacagaa tcttacgcaa
     2401 gaagataaac aagatttgtt taatgacgtg gtgaaagaac tagacttgtt cagagaaaaa
     2461 tatccaattg ttccagaatt agatgatcgt ggagaaataa taagaactac cagtcagata
     2521 aaacaagata ccgaagactc actgcgcaaa gggtggggaa tgaataataa agttagataa
     2581 taatcaaaga agaactaagt aactaccttt aaacggtagt tacttagttc ttctttgatt
     2641 tgcgtgaaat agaggggatt tatttttctg gttacgtgat taaaaaatcc aaaaaatgag
     2701 cataggaacg gttatgattt tagggtgtgt tttaatgaga tattgtgatt tcattaataa
     2761 gtgtatgata atctgttgtt gagttagtaa ataagtaata gagctaactc taaaataaaa
     2821 ctattaggag gagatgtaga tgaaaaagtt taaaaagaaa atatatttag gattggttgt
     2881 tggggtgagt atgtttgggt tcatggctca tactaaacaa gtaactgcag atactaacca
     2941 aaaagaaagg attatgagcc aattagatga ctctcaaata gagtatgtac ccaatgagct
     3001 aattgtcagt ttaaaggcta cacaaagaat ttcaacagag aaaaataaac cgcaaaattt
     3061 attgggtgta aaagttatgg tggaaaaaga tttgactctt aggacaaatg aaagcgatta
     3121 taataagcga gatcaagtat tattagtgaa attgaacgaa gaaattagtg tggaagaggc
     3181 agtaagaaca tttaatgctt ctgatatggt caattatgca gagccaaact atgtggttga
     3241 actagagccg ttgaatgatg ttgaggagcc aaatacaact gattctttag gaaatcgata
     3301 acgatcctaa ttggagtaaa caagctaatt taagagagat tagcatgcca tccgcttggg
     3361 aagaaaccaa gggttctaag gatgtgaagg tagcggtcat tgattcagga attgattatc
     3421 aacatgaaga tctttctgga aatgtagatg aaagtttggg atacgatttt ttttactttt
     3481 ttcaagaaaa taataattca gaatattctg actatattta tgcaacacta gtgactttag
     3541 gagaaacatc aatagccttg ctaaagtttt tgctttcgct aaagagagta ttttcgataa
     3601 tttgtttttt tctaaaatac ctcgttcctc aatatttggg atatggatat gtatagggat
     3661 tcttcgcata aaggtttcca gaaaatcctc tttgccttct gtagtcgcaa aaactaaacg
     3721 tacattggag gcgtgatgga tagtttcacc gactctagaa taagttccgc catccatata
     3781 aacaaatagt ttctcttgcc cttcaggagg caatctgtgt acttcgtcta aaaacagtac
     3841 tccaccgtct gcatcttgca acaatccatt tcgatcttca tctgcgccgg taaaagcacc
     3901 tttaacatag ccaaatagta tccccgacaa taattcagga ttattatggt attgcgcaca
     3961 attaagtact ttaaaaggag cattctcatg aataacgttc acttttttca taaactgata
     4021 agcagtatga acaaaaaaac tttttccaac tccagtatct ccagtgatca tcataggaag
     4081 ccctcttcca ggatagaaga cagcagtttt catttgttcc accgcatcgg ataagctcat
     4141 atcataccca atcatctttt gtagaatgga atctgtagaa ttgggcccca agtctgtttt
     4201 taattcagat atactttcgt atacatcttt taccggaatc aaaaagtgct cttcaaagcg
     4261 ctctttgatg gcaaataaca ctggacgagt attgatttta aatagaacgt tctttgcaac
     4321 taagtcattg gcaagtttac tggcatttgt tcttcccgtt cttaaaaatt gcgacaggtc
     4381 atttgtgtca aaaaagcact cttctaaatt actgatgcca atttcttcac attttgtgtt
     4441 tgcataagtc actatatcat ttttcatctt ataaacagtc ctttctaaac ttctatccct
     4501 taaataaata gcaaaaaaaa ggaagcaata tcgatatgct gtcctcttaa tttaataaag
     4561 cgctaatcta attcactgta ttatcatttt ttagtagtat agcacaatta tgaatgcctt
     4621 ttctttatat caaataaagt ttatataaaa aacctcatcc tgcaagaaag aaaaaatgaa
     4681 cccattcttt ttatactagg tcttaaaaag acagaacatc tgctagaaga tagaagagaa
     4741 taagagttat atatgttgga agatattctt agaaaaaaga caaaactttt agaacacgaa
     4801 aactatattg aacgaataaa atgaaaaaat gattaaaaaa tcattttatt atttacgttt
     4861 acattctaat atgttaaatt gaatataact taaacaggca tgtgactggt aatgcaggca
     4921 ggacctaaat aagttgaata acaatgtatg cgtttcaata cacgtttgcc ttgttgtcaa
     4981 tttatttagt ttctgcctgt tttttgtgtt gaggatggcc acttcgactc aaaagttcat
     5041 cactcaaaaa agaaaagagg gaaacaccat gaattacaaa caaacagcag aagaagtatt
     5101 gcagtacatc ggtggggaag aaaacatatc ccatttggag cattgctcaa ctcgtttacg
     5161 ttttacgtta aaagacaaca gcaaggtaaa tatgagccag ttagaaaaag tggatggcgt
     5221 tatgggcgtt cgccaaaatg tgcaatgcca agtaatcatt ggaaacgatg ttgttgaagt
     5281 gtatgatgaa gtgaaagcgt tattaggtga caatgtttca aatactgaag aagcaccaaa
     5341 acaaaaacaa aaattaggtt cagttctatt agattttatt atttctattt tccaaccttt
     5401 aattcctgcg attgcaggtg gaggagtatt gaagtccttg ttgctactag cttctatctt
     5461 agggctgatt agtgaccaat ctcaaaccta tcagatttta aatttggtcg gcggggcacc
     5521 gctttatttc ttgcctattc tggtggcaat gactacggct aataaattga aagtcaatca
     5581 attggttgca gtatctgctg tcggtgcatt gatccttcct gaaatgacag ctatgttggc
     5641 ggaaggtgct acattcttat cttttggatt ggataatata gcgtatgcat ctcaagtatt
     5701 cccagccatc ttaactgttt tattttatgc tcaacttgaa aaatggttta caaaagtttc
     5761 accaaaacca attcgtattt tctttgtacc tatgatgagc ttattgatta cagttcctat
     5821 tgctttgttg cttctaggac cattaggtta taatgtagga acagctttct cttctatcat
     5881 tgtgtctttg tacaatcaat ttggatgggt tgcaaccgga gtcttagcag ctgtactgcc
     5941 attaatggtt gtaacgggta tgcataaagc aatgatccct tacgctgttt catccatgag
     6001 tgaattgggt gcagaacttc tttatctccc ggcttcattg gcacacaata tttcagaagc
     6061 tggcgcatgc tttgctgtca gtatcaaaac aaaagacaaa aaattgaaat ctacggctgt
     6121 ttctgcgggt atatctgcat tattcggaat tactgaacca gctctttacg gggtaacgat
     6181 tctgcacaaa cgtgttttat atgccgttat gttttctagc ctgatcgggg gagcatttgc
     6241 aggaatcgtt gcacttcaag cctttgcttt agtgggtcct gggattgcaa gtatcacgat
     6301 gttcgctgac aaagctgacc caatgaatct ggtttgggca tttgcagtat cggggatttc
     6361 atttgtggtt gcttttgctg cagcgttggt tctttataaa gataaagaag ttgtcaatga
     6421 agttgaaact aagatggaaa cggccgttgt ttcagatgct gttctttcaa gtcccgtgaa
     6481 tggaaaagta atctcactat cagaagtaaa agacactgtt ttttctagtg gtatggtagg
     6541 tgatggagta gcaatcattc ctgaagaagg gatattagta gctcctgaag atggagaaat
     6601 cacaatggta tttgaaacga agcatgctat tggtttaaaa acggctaatg gtgcagaact
     6661 tctttttcat attggtattg atactgttca gttagaagga aaagggtttg aggcatttgt
     6721 tcgttctggt gaaaaagtag ccaaaggaca aaagctgatt gcatttgata ttgatttaat
     6781 caaagaagca ggattcgacc ccacagttat ctgtgtggta accaataagg ataattacac
     6841 tatccgttca tttaacctag aaaaattggt aaccaatgat agtgatctct tagctatcag
     6901 tccgatagct taaacacaag atagtgaaaa aaaatatatt gaatgaagga atagaggaga
     6961 atagaataat gacaaacaca acaagttttc caaaaggatt tttatggggc ggcgcgatag
     7021 cagcaaatca agtagaaggt gcttggaatg tagacggtaa aggtctttca gtagcggata
     7081 tggctactta taaaccaaat ttagacaaca aagattttgc tgctcatatg catgtcaccc
     7141 tgcagtctat aaaagaagct agtgaagaca aatctgataa atggtaccca aaaagaaggg
     7201 gcgttgattt ttatcatcac tataaagaag atttagcttt atttgccgaa atgggcttta
     7261 aaaccttgcg cctctctatt gcttggactc gcttgttccc tacaggagaa gagctggaac
     7321 cgaatgaaaa aggggttgca ttttatgaaa acgtctttaa agaaatgcaa cgtttaggaa
     7381 ttgagccgat tgtgacacta tctcattatg aaatgccatt agctttagca gttaaatata
     7441 atggctgggt ggagcgtcga gtaattgatg attttgttcg cttcgctaac gtttgttttg
     7501 atcgcttcgg caaatacgta aaatattggc tgacatttaa tgagatagac agcatccacc
     7561 gtcatccatt tacaacagct ggaattatcc aagagaaaag cgcagaaggt caagaagctc
     7621 aagatatcta tcaagcattg catcatcaat ttgttgcgag tgcgttagtg acaaaagatg
     7681 ctcatgaaaa aattccagga agccaagttg gttgtatgtt aacgaaattg atgacttatc
     7741 cattaacttg cgcaccagaa gacgttgagc tgaccttgaa aaagaactta gaaaataatt
     7801 tttatgcaga tgttcaagtt aagggagaat atcctgctct aatcgaaaag gatttggaaa
     7861 atcggggaat tcagattaaa atggaagatg aagatcgaga aattttaaag aaacataccg
     7921 tagattttgt ttctttcagc tattacatgt ccatggcaga atcagctgat ccaaacgcag
     7981 aacgcacacc gggaaatact gttttagggg ttaagaatcc tcatttgcct tcaacagatt
     8041 ggggctggca aatcgatcca aaaggtttga aaatttcgtt gatcgaactt tatgatcgtt
     8101 accaagtacc gttgatgatt gtggaaaatg gtatgggagc agtcgatcag gtagtagatg
     8161 gtaaaatcca tgatccgtac cgcatcgact actttaaagc acacttccaa caaatgaaag
     8221 aagcaatcga tgaaggtgta gatttgatag cttatactag ctggggacca attgatttag
     8281 ttagtgcagg aacttctcaa atgtctaaac gatatggatt tatttatgtg gatgcagacg
     8341 atgaaggaaa tgggacatat aaccgcttga aaaaggattc gttcaactgg tacaaagaag
     8401 tgatcgaaac aaacggtgca tccttattcg agtagtctcg atctttttaa gcagcgatag
     8461 ggggataatg agtgatttca ataaaaaaag tattgaattc aagtgtggta cttgttgaag
     8521 aaaatggcaa agaaatgatt gcattaggta aagggatagg ctttggacag aaagctgggg
     8581 atgaaatatc aggagataaa gtagataaaa tatttctgcc ttttgaagaa aagaaatctt
     8641 cacaactatt agagttagtt gaagaaatac ctattgaatt ttttgaaatc actcgtacta
     8701 tcgtttttga agcagaaaaa ggattagata aaaaactgaa tcctggagtc tatttaacct
     8761 taacagacca cctgcatttt gcagttgaac gcgcacaaaa aggggtaaat attgcaaacc
     8821 gtttgtactg ggaaataaaa atctactatc ccaaagaatt tcaaattggg aagtacgcac
     8881 tcaccctttt gaacgagcaa tatggaattg atcttcctaa agaggaagcc tcaaacattg
     8941 ctttccattt gatcaatgca caatcggaag atcctgatag cggcgctgga ttcaagtatg
     9001 caaaaatgat cggcgggatc gtcaatttgg tccggtattc gttgcagaaa gatatcgata
     9061 ctaattcgat ccattatgcc cgcttcatta cacatgtacg tttctttgta gaaagatatt
     9121 attctgatgc tttgttggat gatggtgagg aagaattcta caatcaaatg tggatcttat
     9181 acccgtctgc catggaaagc gcagctaaga tcaaagacta cattgaaaaa gtttatcata
     9241 aaacaattcc gaacgatgaa gttgtatacc taggtgtcca catcaatcgg ttgatgaaac
     9301 atgctaatgg gtgagcgctc agtaatacta ttgatgcttt taaaatcttt agagatggat
     9361 tatagtaaaa acaaggtgat ttccagctgt ttgttatcta taagagacat ttaaaaagga
     9421 ttaacgtaag ataataaaaa atct
//
LOCUS       DAZKKW010000094         9443 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.096, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000094 DAZKKW010000000
VERSION     DAZKKW010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9443
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.096"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(78..839)
                     /locus_tag="ACG8PO_002316"
     CDS             complement(78..839)
                     /locus_tag="ACG8PO_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HFQ0207604.1"
                     /translation="MHTPEEIMELSIHLGTKKIHKPILAKLILGFIGGAMISLGYLAY
                     VRVSASIPESMASLTSLVGASVFPIGLLVILLGGGELITGNMMAVAIAFMDKKVTFSQ
                     LVKNWSVITFANIIGAIFVAYFFGHFVGLTHSGVYLDQVIHIADHKIAVSWWQALGSG
                     IGCNWFVGLALWLSYGAKDAAGKVLTIWFPVMIFVAIGFQHSVANCFVIPAAIFEGQA
                     TWLEFLSNFIPVYIGNIIGGAIFVSGFYYVSYKHH"
     gene            1159..1404
                     /locus_tag="ACG8PO_002317"
     CDS             1159..1404
                     /locus_tag="ACG8PO_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0207605.1"
                     /translation="MSKSKKNITIIGLGLTVYLFTEFVYRHVNLFAEPLFVLYESNSS
                     IVSELANLMIALLFTPALRVITILVCLVLLFFVNKKK"
     gene            complement(1588..2337)
                     /locus_tag="ACG8PO_002318"
     CDS             complement(1588..2337)
                     /locus_tag="ACG8PO_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFQ0207606.1"
                     /translation="MLKLERHQKILDLLNAEQKVIASDLSQRFNVSEDTIRRDLKDLD
                     SQGLLKRVHSGALRVGPPITNFNYRETVASEQKTALAHKALPFLKEESVIIIDGSTTN
                     LALAKEIPQDFRATIITNSPPIAIALAQHPNLEIINIGGTFYKQSMINIGVQAYKQLQ
                     NIRADLYIMGIYNIDLDVGTSVPTMQESDIKREMIRISTEVLGMITNDKFGTISNHIV
                     GPIEDLTYLISEEIPEKVRKEYNKQKIVLID"
     gene            2523..3227
                     /locus_tag="ACG8PO_002319"
     CDS             2523..3227
                     /locus_tag="ACG8PO_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFQ0207607.1"
                     /translation="MEKKIILSPSLMCGDLANLEGSIQELESIGMDSLHIDVIDGSFS
                     PSMPLGIETIKRIREITSMNFDVHIMAKDNDNEFFIGEMLKIGVESITFHYETSLHID
                     RYIQLIKNSGTKVGIALNPATPLNVLQYILPEIDRICLMLINPGFATNKSEKQVSYAL
                     KKITDLCQLVNEKELAVDIQVDGRVSLETIPGLLDAGATNLVLGSTSLFLKGNTLAEN
                     QKLLYQAMKQSVRKNQ"
     gene            3267..3824
                     /gene="hxlB"
                     /locus_tag="ACG8PO_002320"
     CDS             3267..3824
                     /gene="hxlB"
                     /locus_tag="ACG8PO_002320"
                     /EC_number="5.3.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0043800 - hexulose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-3-hexuloisomerase"
                     /protein_id="HFQ0207608.1"
                     /translation="MSYKAAKQNIVREITETLDRISSKDVEQLIKKIEKAERVFFVGV
                     GRVLLALEGMAKRLGHLGIQTVVVGQITEPAITDKDLLIVGSGSGESAFPLTIAKKAK
                     EFGSQVAHIGSNPDSTMKEYSDLFIRIPVQTKLALPEEISSIQPMTSLFEQSVLLLGD
                     SIALVMIEEKAIDMSKLWQYHANLE"
     gene            3832..4971
                     /locus_tag="ACG8PO_002321"
     CDS             3832..4971
                     /locus_tag="ACG8PO_002321"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008926 - mannitol-1-phosphate
                     5-dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0019594 - mannitol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFQ0207609.1"
                     /translation="MKAVHFGAGNIGRGLIGQILHQNGFDLCFIDTNPELIHQLNQDK
                     GYTIEIIDEMNRSDFIDHVVALNSLSELEEVITATKNADILTTSVGAINLVKIAPIIA
                     QALSKRFEQKHSINIVANENVINASDVLKKEIYSLLSEKEQENFDEYAYFVNTAIDRQ
                     SLGKVLGGKMISVVEPYYEWIMDRQQLDPTTSFDMKNVNLVDDMEPYIERKLYIVNAA
                     HAAVAYLGALKGYKTIQEAIQNMEIFNLVKQFLMENIEYFVQKYHFSREELTSFVEKT
                     LNRHGNQKLSDEITRVGSSPIRKLGPNDRLIAPIVKLDSYHLPYDAGIKVIAAGYHYR
                     NLADPEAEQLRRMIVNDGLKATIKKISRLEGKLLNEVVAAYESQI"
     gene            4983..5414
                     /locus_tag="ACG8PO_002322"
     CDS             4983..5414
                     /locus_tag="ACG8PO_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFQ0207610.1"
                     /translation="MLKEQLSGNTMFMDAIDSWQEAIRVSARPLIGKGIIEESYVQAM
                     IQNVLDNGNYIILLPLVAMPHARPEFGSKGVGMSFLCLDKPVMFPSEEPVKFFFTFSS
                     DSPKGHLDLMANLGELLSDEKMYQKLFDVKTEKALLDLIKK"
     gene            5428..6873
                     /locus_tag="ACG8PO_002323"
     CDS             5428..6873
                     /locus_tag="ACG8PO_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HFQ0207611.1"
                     /translation="MEVANKKKRVSMRAKVQAIGGFLTNMVLPNIGAFIAWGIVTALF
                     IPTGWFPNESLNELVSPTVSYLLPLLLAYTGGRMVGGTRGAVLGTIGTIGLIIGADIP
                     MFMGAMIMGPLGGWMMKKTDDFLDGKVPAGFEMVVNNFSIGIIGFILMCLSYLVVGPI
                     IQSANQFVTSAIEALVSTGFLPILSLINEPAKVLFLNNVIDQGVYYPLGMQQTLEAGR
                     SIYFMVASNPGPGLGLLLAYSLFGKKAAKQTAPGAIIIHFFGGIHEIYFPYVLMKPLT
                     ILSMIAGGMTGIITFELFDAGLVAGPSPGSIFSYLALTPRGNFLGVMAGVVTATLVSF
                     FVTSVILKADKSTDEDRNFELSVQKSKTMKSEGRKLLKEENAREICTEKKEMNKISFA
                     CDAGMGSSAMGATIFKKKLQKAGITDVEIKNYRIEEVPEDSDLVIVHQDLEERTRKAH
                     PNMKIVTLKNYLQDPVINGLAEEIIGLKTED"
     gene            7159..8784
                     /locus_tag="ACG8PO_002324"
     CDS             7159..8784
                     /locus_tag="ACG8PO_002324"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFQ0207612.1"
                     /translation="MKKIYSKEIDCFRGIAVLAVIFYHLPNSLLPGGLQGVTIFFVVS
                     GFLMAKNTIRDEKNRQFSFLHFYYKRIKRIYPFLLFSLVVFIFILGISNIRLIGNIRS
                     ELPSLIFGYNNWWQLNQGVSYFDSYFNASLFKHYWSLSVELQFYLIWPFLFIVIKRMR
                     RKQVFYLIYTLIFVSILFSLFLPSTKAYYHTIAKLFPFLLGVWGYFNRITIGRFFEQN
                     TFSKIWLLLLASLCLILFPIFPYTLNELLISICFALLLASVDDMNIAKKTKIASTKLS
                     FVGKISYELYLVHFPVLIALTNLFKHHHWKYLDIAGLFTVFLCTLLMITCFRKGVQKM
                     RSPKIWTAMALMIIGVSVLVLRAPASRLTADQNQLQSLLKENSSKANAGENLLSQEKK
                     ILFIGDSVMLGAYQELKETFKKEALVDAKESRQITALPEILKEHKDLVSYQQIVIGLG
                     TNGVITDEAIEQTMSLLKDKKVYWVNIKAPTGWQDTINATLATLPNQYSNITMIDWYS
                     ESKNHPEFFYDDETHLNESGRTAYAKYIASVIQ"
BASE COUNT         3236 a         1402 c         1838 g         2967 t
ORIGIN      
        1 aaaggctatt gttcttaaaa tataaatagt atataaagag attgaaggta atgctcgaat
       61 gaacattacc ttcaatctta atgatgctta taactaacgt aataaaaacc tgaaacaaaa
      121 atagctccgc cgatgatatt tccaatataa acggggataa aattcgagag aaattctaac
      181 caagtcgctt gaccttcaaa tatggcagca ggtatcacaa aacagttggc cacactatgc
      241 tggaaaccga ttgccacaaa aatcatgact ggaaaccaaa tcgttagtac tttgccggcc
      301 gcatcttttg ctccatatga taaccataac gctaaaccga caaaccaatt acaacctatt
      361 ccggagccta atgcttgcca ccaagaaaca gcgattttat gatcagcaat atgaatcact
      421 tggtcgagat agacacctga atgagttaaa ccaacaaagt gtccaaagaa ataggcaacg
      481 aaaattgcac caataatatt tgcaaaagta atcactgacc aattcttaac cagctgtgag
      541 aatgtgactt ttttatccat aaaggcaatc gccacagcca tcatatttcc ggtgatcaat
      601 tcaccaccgc caagaaggat gaccagcagg ccgataggaa aaaccgaggc accaactaaa
      661 ctagtcaagc tagccataga ttctgggata gaagcagaga ctcgtacgta tgccaaataa
      721 cctagagaaa tcatggctcc tccgataaaa cctaaaatca gtttagctaa gataggttta
      781 tgtatttttt ttgttccaag atgaatactg agttccataa tttcttctgg tgtgtgcata
      841 atacttcgat tcctactttc ttaatgaatt ttcatgtgat aaaatatagt gaaaaaaatc
      901 acattttctt tgttgattat atcatcttat tatttatatg tgcatattat tgtaaaattt
      961 tctatttaat cacatttgat tctaaaaaag aaacaaaaat aaaagtcatg tatatggtgg
     1021 tcaaaaagta aataatatca gttatcaaat ttcctgtaaa ttttcttgaa actacagcat
     1081 attattactt caaaacaatc tacatttttt ataaaaaagg aaatgaataa ctgaaaaaat
     1141 tattatagga gagaaaaaat gtcaaaatct aaaaaaaata tcaccataat tggtttaggc
     1201 ttaaccgtat atttatttac agaatttgtt tatagacatg tgaatttatt tgcagaacct
     1261 ttatttgtat tatacgaatc aaattcctca atcgtcagtg aacttgctaa tttaatgata
     1321 gcattgctgt ttactcccgc gttgagagtc ataactattt tggtctgttt agtgctattg
     1381 ttttttgtta ataaaaagaa ataacgtagt atttcaaaag acaaagaagt gggatgatga
     1441 ttgctatagt atgttatacg tggtgctagg taagtgagaa taaacaaaaa aggaaacacg
     1501 caataaagtt tatttcaaaa ctttattgcg tgtttctttt ttagattttt tatatctgtt
     1561 gctttagtag ttaccaatag ttttcgctta atcaatcaaa acgatttttt gtttattgta
     1621 ttctttacga accttttcag gaatttcttc tgaaataaga taggtaaggt cttcaattgg
     1681 accgacgata tgattactga tggtgccgaa tttatcatta gttatcatcc caagtacttc
     1741 agtagaaata cgtatcatct cacgtttaat gtcggattct tgcatagttg gcacgctcgt
     1801 tccaacatct agatctatgt tataaatacc cataatataa aggtcggctc gaatgttttg
     1861 tagttgctta taagcttgta ccccaatatt gatcatagat tgtttataaa aagtaccgcc
     1921 aatattgata atttccaaat taggatgctg tgctaatgct atggcaatcg gcgggctatt
     1981 ggtaatgatt gttgcacgaa aatcttgtgg gatttctttt gctaaagcca agttagttgt
     2041 acttccatca atgatgatga cagattcttc ttttaaaaaa gggagggctt tatgagctaa
     2101 tgcagttttt tgttcgctag ctacagtttc tcggtagtta aaattggtaa ttggcggacc
     2161 aacacgtaaa gcgccgctgt gtactctttt taagagacct tggctatcca aatctttcaa
     2221 atctcgtcga atcgtgtctt cggatacatt gaaacgttga cttaaatcac tagcgataac
     2281 tttttgttct gcgtttaata aatctagaat tttttgatga cgttctaatt tcaacatgtt
     2341 gcgtgctcct tcttatttat tcttgtttat catgtcattt atgcgttttt atgtcaataa
     2401 tttccgtttt taatacacta aaaaggcata aacaagcaaa aacaaaatat ttttatgcag
     2461 ttttatgttg actagtattt gttaagcggt tacaatgagg gggtaattaa aggagggaat
     2521 gtatggagaa aaaaattatt ttgtcgcctt cacttatgtg tggggatcta gcaaatttgg
     2581 aaggaagcat ccaagaacta gaaagtattg ggatggattc cttgcatatt gacgtgattg
     2641 acggttcttt tagtcccagt atgcctttag ggatcgaaac aatcaaaaga attagagaaa
     2701 tcacttcaat gaattttgat gtacacatca tggctaagga taacgataac gaatttttta
     2761 ttggagaaat gttgaaaatt ggcgtagaga gcattacttt tcattatgaa acaagcctcc
     2821 atattgatcg atatatccag ctcatcaaga attctggaac aaaagtaggg atagctttga
     2881 atccagcaac tccgttaaat gtattacaat atattttacc cgagattgat aggatttgtt
     2941 tgatgctgat taatccaggt tttgcaacaa acaagtcaga aaaacaagtt tcttacgcgc
     3001 taaaaaaaat tacagatctt tgccagttag taaatgagaa agagttagca gtagatatcc
     3061 aagtggatgg acgggtatcg ttagaaacga tcccgggatt attagatgca ggcgcaacaa
     3121 atctggtatt aggaagtaca agtttgtttc ttaaaggaaa tacattagca gaaaatcaaa
     3181 aactacttta tcaagcgatg aagcaatcag ttaggaaaaa tcaatagatg tagtggtcct
     3241 gaataataga aatggagggg aaatagttga gttataaggc ggcgaaacaa aatattgtaa
     3301 gagaaattac tgaaacatta gaccgtattt cttcaaaaga tgtggaacaa ttgataaaga
     3361 aaattgaaaa agcggaacga gtttttttcg tgggggttgg cagagtactg ttagcgctag
     3421 aaggaatggc aaaacgctta gggcatctag gaattcagac agtagtcgta ggacaaatca
     3481 ctgaaccggc aattacagat aaagatttat tgattgttgg ttctggtagt ggggaaagtg
     3541 ctttcccact aactattgca aaaaaagcaa aggaatttgg gtcccaagtt gcccatatag
     3601 gatcaaaccc agacagcaca atgaaagaat attcagatct ttttattcgt atacccgtac
     3661 aaacaaaatt agcgttacca gaggaaattt cttctataca gccaatgact agcctgtttg
     3721 aacaaagtgt tcttttgtta ggagatagta ttgctttagt gatgatagaa gaaaaagcaa
     3781 ttgatatgtc aaagttgtgg caataccatg ctaacttaga gtaggtgaaa aatgaaagcg
     3841 gtacattttg gagcagggaa catcggcaga gggcttattg gacaaattct acatcagaat
     3901 ggattcgatc tatgttttat tgatacgaat cctgaactga tccatcaatt aaatcaagat
     3961 aagggttata ctatcgaaat tattgatgaa atgaacagga gtgatttcat tgatcatgtt
     4021 gtggcactga atagtttatc cgagctagaa gaggtgatta cagcaacaaa aaatgcagat
     4081 atcttaacaa catctgttgg cgcaataaat ttagtaaaaa ttgcaccgat cattgcgcaa
     4141 gccttaagta agcgatttga acaaaagcat agcatcaata tcgtagctaa tgaaaatgtg
     4201 ataaatgcaa gtgatgtgtt gaaaaaagaa atttattctt tgctgtcaga aaaagaacaa
     4261 gagaattttg atgaatatgc atattttgtg aatacggcaa tcgatcgaca atctttagga
     4321 aaagttcttg gaggaaaaat gatctctgtt gtcgaacctt attatgaatg gataatggat
     4381 cgacagcagt tagatccaac tacatctttt gatatgaaaa atgtaaattt ggttgatgat
     4441 atggagcctt acattgagcg aaagctttat atcgttaatg ctgctcatgc ggcagtagct
     4501 tatttaggcg cactgaaagg atataagacg attcaagaag caatccaaaa tatggagatt
     4561 ttcaatctag tcaagcaatt tttaatggag aatatagagt actttgtaca aaaatatcat
     4621 ttttctagag aagaattaac atcttttgtg gaaaaaacat taaaccgtca tgggaatcag
     4681 aaactctccg atgaaattac tcgtgtagga agttccccta ttcgaaaatt aggccctaat
     4741 gatcgtttaa ttgctcccat agtaaaacta gattcatatc atttacctta cgatgcagga
     4801 ataaaagtaa ttgctgcggg atatcattat cggaatcttg ctgatccaga agctgaacaa
     4861 ctacgtcgaa tgattgtaaa tgatgggttg aaagcaacga ttaaaaagat ttctcgtttg
     4921 gaaggtaagc tgctgaatga agttgtcgct gcttatgagt cccagattta gaggaggaga
     4981 atatgctaaa agaacaattg tcaggaaata caatgtttat ggatgcgatc gattcatggc
     5041 aagaggcaat ccgagtaagt gcgcgtcctt tgataggaaa gggaataata gaggaaagct
     5101 atgtacaagc gatgatacaa aatgtgttgg ataacggaaa ttatattata ttactgccac
     5161 tagtagcaat gcctcatgcg cgtccagaat ttgggagcaa aggggtcggg atgagctttt
     5221 tatgtttaga taagccagtc atgttcccaa gtgaagaacc agtaaaattc ttctttacct
     5281 tttcttctga ttcgccaaag ggacatttag acttaatggc caatcttgga gaattgctga
     5341 gtgatgaaaa aatgtatcaa aaattattcg atgtgaagac agaaaaagca ttgttagatt
     5401 tgataaagaa gtaaaggaga gaagaaaatg gaggtagcaa acaaaaaaaa acgagtctcc
     5461 atgcgagcta aagttcaagc gattggcggt ttcttaacaa atatggtatt gccaaatata
     5521 ggcgcattta ttgcatgggg aattgttact gcattattca ttccaactgg gtggttccca
     5581 aatgaatctc tgaatgaact cgtaagtccg acagtcagct atctattgcc gttactactt
     5641 gcctatactg gtggtcggat ggttggtggt acacgtggag ctgttttagg tacaatcgga
     5701 acaataggtt tgattattgg tgctgacata ccaatgttca tgggagcaat gattatgggg
     5761 ccacttggtg ggtggatgat gaaaaaaaca gatgattttt tagatgggaa agttcctgct
     5821 ggttttgaaa tggtcgtcaa taatttttct ataggaatta ttggtttcat actgatgtgc
     5881 ctttcttatc tggtagttgg accaataatc caatcagcta atcaatttgt aacgagtgca
     5941 attgaagcat tagtttcaac tggtttttta ccgatactgt ccttaatcaa cgaaccggca
     6001 aaagtcttat ttttaaataa tgttattgac caaggtgttt attatccatt aggtatgcaa
     6061 caaacattag aggcaggcag atcaatttat ttcatggtag catcaaatcc tggccctggc
     6121 ttaggactat tgttagctta ttctttattt ggtaaaaagg cagcaaaaca gacagcacca
     6181 ggtgcaatca tcatccattt tttcggcgga atacatgaga tttattttcc atatgtatta
     6241 atgaaaccac tcactatatt atctatgatt gctggtggaa tgacaggaat catcactttt
     6301 gaattatttg atgcgggcct ggtagcaggt ccaagtccag gttctatttt ttcttattta
     6361 gctctaacgc ctcggggaaa ttttttgggt gtcatggcgg gagtggtcac agcaacactg
     6421 gtttcctttt ttgtaacatc agtaatttta aaagcagata aatcaacaga tgaagacaga
     6481 aattttgaac tatcagttca aaaatcgaaa acgatgaaat cagaaggaag aaaattatta
     6541 aaagaagaaa atgctagaga aatatgtacc gagaaaaaag agatgaataa aatttccttt
     6601 gcttgtgatg caggaatggg aagcagcgca atgggcgcaa caatattcaa gaaaaaattg
     6661 caaaaagcag gtattactga tgttgagata aaaaattatc gaattgaaga ggtaccagaa
     6721 gatagtgatt tggtgatcgt acatcaagat ttggaggaac ggactagaaa agcacatcct
     6781 aacatgaaaa ttgtgacatt aaagaattat ttgcaagatc cagtaatcaa tggattagca
     6841 gaagaaatca ttggattaaa aacagaagac taatttatgg gaatttttga ttaaccttta
     6901 ttaaaattat tttgcctaat ataaaaaggt tgtgacatgg cttgtgtcgc aacctttttt
     6961 tgtcgcacaa ataattaatc ggatatttta tgttcatatt ttttgtgatc aacgagaact
     7021 gatggggcaa agatacattt tagatacttt taactgttaa tttacaaaac aaggtgaaac
     7081 gaatagaaaa atgaaaaaat cgatacggaa gaatatgttt gattaaaaca tgcatgaaga
     7141 gaaggaggtt gatgatacat gaaaaaaata tattcaaagg aaatcgattg ttttagaggc
     7201 attgctgttt tagcagtgat attttatcat ttgccaaata gtttgctgcc aggcggattg
     7261 caaggagtaa cgatcttttt tgtggtttct ggttttttaa tggctaaaaa tacgataaga
     7321 gatgaaaaaa acagacaatt cagtttttta catttttact ataagcgtat caaacgtatt
     7381 tatccatttt tactgttttc tttggtcgta ttcattttca tactagggat tagcaacatt
     7441 cgattgatag gaaacattcg tagtgagcta cccagtttga tttttggcta taacaattgg
     7501 tggcagctga atcaaggagt atcttatttt gactcttact ttaatgcttc tttatttaaa
     7561 cattattggt cgctttcagt tgaactacaa ttctatttga tatggccatt tttgtttatt
     7621 gtcattaagc gtatgagaag aaaacaagtg ttttacttaa tctatacatt gatttttgtt
     7681 tctattcttt ttagtttgtt tttaccttca actaaggcat attatcatac gatagctaaa
     7741 ctttttccat ttttattagg agtttggggg tattttaatc gaataactat tggccgtttc
     7801 tttgaacaaa acacttttag taaaatctgg cttttattac tagctagtct ttgtctgatt
     7861 ctgttcccga tttttcctta tacactaaat gaactactga tcagtatttg ttttgctcta
     7921 ttgttagcca gtgtcgatga tatgaacatt gcaaaaaaga ccaaaatcgc tagtacaaaa
     7981 ctttcttttg taggaaaaat cagttatgaa ctttatttgg tgcatttccc agtattgatt
     8041 gctctgacaa atctattcaa acatcaccat tggaaatatt tggatatcgc aggtctattc
     8101 actgtgtttt tatgtacatt actcatgatt acttgcttta gaaaaggagt gcaaaagatg
     8161 agatcaccca aaatttggac agctatggca ctaatgataa tcggagtttc tgtactggta
     8221 ttacgtgctc ctgccagtcg actgaccgct gaccaaaatc agttgcaaag tttattaaaa
     8281 gaaaactcta gtaaagcaaa tgcaggagaa aatctgttgt cacaagagaa gaaaatatta
     8341 tttatcggtg attctgttat gctgggggcg tatcaggaac taaaggagac atttaaaaaa
     8401 gaagcattgg tagatgcaaa agaatcaaga caaatcactg cgttaccaga aatcttaaag
     8461 gagcataaag atttagtttc ttaccaacaa atagtgatcg ggttaggaac caatggtgtg
     8521 ataacagatg aagctataga acaaacaatg tctttgctga aagataaaaa agtatactgg
     8581 gtaaatataa aagcaccaac tggctggcaa gatacgataa atgccacatt agctacattg
     8641 ccaaatcagt attctaatat aacgatgatc gattggtata gtgagagcaa aaatcatcct
     8701 gaattttttt atgatgatga aacccatttg aatgaaagtg gacgtacagc ttatgcaaag
     8761 tatattgctt cagttattca ataaggtagt gattacgtgt gttaacctta ctttttccta
     8821 ataggtgaat caatttaaat aaaaaatact tcatatgata aacggtgttt aagaggagga
     8881 gctttttaat attcgtggta aaaaagagat gggcgaggtc gttaactgag agcttcttgg
     8941 aaagaaatcg ctttggattt atcctctttg ttatacagaa catgagcgag acgacaatac
     9001 taggatgtat gttctgatcg aagttttatg gttacaagca gcaatcagct tagataaaat
     9061 taaggatcgt aggaaccaag taaagaaggc tgttagtaaa gaaacaaacg atcaagtatg
     9121 ttttactctt aagaagatgc agtgaataaa taatagtaat ataatgctag aagggactgt
     9181 gacaaaagac gtgtcacagc cttttttgtt attttaatta tcgtccagtc tttttttata
     9241 aatgattgct taagcattaa atattaaaaa atatagagtt caaaattact gcacaattag
     9301 atgaaaaatt tgaacaacaa aaagtgtatt tttatactat aatagctttt gaatttagtt
     9361 actctattca aaagttatgc tatggacaag aaaaagaata aatagaggtg agaaaatgaa
     9421 tagtttcaag aaaaaatttg tga
//
LOCUS       DAZKKW010000095         9395 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.097, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000095 DAZKKW010000000
VERSION     DAZKKW010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9395
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.097"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(126..794)
                     /locus_tag="ACG8PO_002325"
     CDS             complement(126..794)
                     /locus_tag="ACG8PO_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFQ0207613.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            928..1215
                     /locus_tag="ACG8PO_002326"
     CDS             928..1215
                     /locus_tag="ACG8PO_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0207614.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            1631..3169
                     /locus_tag="ACG8PO_002327"
     CDS             1631..3169
                     /locus_tag="ACG8PO_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HFQ0207615.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            3166..4131
                     /locus_tag="ACG8PO_002328"
     CDS             3166..4131
                     /locus_tag="ACG8PO_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0207616.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            4118..5440
                     /locus_tag="ACG8PO_002329"
     CDS             4118..5440
                     /locus_tag="ACG8PO_002329"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFQ0207617.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      5503..5607
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            5681..6424
                     /locus_tag="ACG8PO_002330"
     CDS             5681..6424
                     /locus_tag="ACG8PO_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0207618.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            6439..7431
                     /locus_tag="ACG8PO_002331"
     CDS             6439..7431
                     /locus_tag="ACG8PO_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0207619.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            7443..8180
                     /locus_tag="ACG8PO_002332"
     CDS             7443..8180
                     /locus_tag="ACG8PO_002332"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0207620.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLPGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            8170..8892
                     /locus_tag="ACG8PO_002333"
     CDS             8170..8892
                     /locus_tag="ACG8PO_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFQ0207621.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(8976..>9395)
                     /locus_tag="ACG8PO_002334"
     CDS             complement(8976..>9395)
                     /locus_tag="ACG8PO_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HFQ0207622.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTEK
                     QAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYL
                     NGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT         3080 a         1612 c         1976 g         2727 t
ORIGIN      
        1 actattttcg cagatcgttt gcaaaaatag tttatttttt tataaagaag attttactga
       61 ataatatcat tttagtttac ataatgatat ttctgtaaat taaataaagt agtgaaaaat
      121 aagaatcagt gctctaactg ttcaagagga tcaagtggtt caaatgtttc tgtagtctgt
      181 tttgcttcca attggggcaa aacagatcca atttccagat acttcacttt attcaatgta
      241 taagatagaa tagaacagct aagtaatgta tacagaaaat gtgtgagatc caaataaagc
      301 aaggagacaa ctaaaatacc aaaagtaatc aagaaaagac ttttgccaat cttaatatga
      361 aaatattttt caatggcttg ataaatgata tctgttccgc ctgtgcttcc gccgaatcga
      421 aagacgagac cgcaaccaat gccagaaaga atacctgtca tcaaactatt gatcagtaga
      481 ctatctacag caaaatgtat atgaaggctc tcccataaat gtaaaaagaa aactagcgca
      541 aatgttccga cgatcgtccg gagaaaagcg tgttttccaa aaagaaacaa tgagataagc
      601 aataaaggta aattaaaaag cagtgaagtg acagaaggag cgaatccaaa aagattatgc
      661 aaaagcaaag caataccagt aaccccacca tctgccagct tagatggaat attgattcct
      721 gtcacagcta atccgtagat tcctgcgcct aaaatgattc cgcacaattc tgtaaataaa
      781 tttaattttt tcatttctag cctcttttct tttgaaaatt ttcagcaact tctttattat
      841 actgacttat actcttactt caagaggaaa atgaaaaaaa attatttttg gctgtttctt
      901 tttatttatc aaataaggag tggaaaaatg cgagcagcag acttattgac aattcttaca
      961 aaatcaccca aaaaaacaga gtatcaagga gtttttttgg ctcaagaaca aaaactggtg
     1021 cctgtgacaa tggtccagat aaataaagca aatcaattta tctttttatt tgaacacggc
     1081 aaagcaccac ttgctatgaa agacatcttt ctttttttga tgaagaaccg tgagaaagct
     1141 gtttattatc agtcaaaaga agaactcatt cctctttatg gagtgaaaga gatagaaaat
     1201 aaagtggtga tataagtaaa aataagaaaa caagacgttt atgatgtcaa aacaaccatt
     1261 ttttacattt ttggcaaaaa agtgtcatga tacagtataa tattcgttga acaggccaat
     1321 aaaggtgttc tcccccacga gatttctact ttattggctg tttcttttta ttaaaataaa
     1381 agttgtgtgt gtaaaaaaga tatagctgat tccaagtgac aatttcgtat agatttttat
     1441 ggtattcgta gtgaaaaaac gagaaccaaa aagttgatcc gactttgttg cttggattct
     1501 ttttttgtca gcatatttag aggtattttt ttcttttttt tgttcgtgct gtaaggttaa
     1561 ggcaagacaa gttagttggg aaaaaggaaa gaaatgttta tacttaaaga ataacagaaa
     1621 ggaaggtcgc atgaataatt ttatacaaac attaagtgaa agaaaaggcg agttgctgga
     1681 agctgcattt caacatctgt ccatttctct catttctttg ctgattgcag cactgattgc
     1741 tattccttta gcgatttggg cagccaatca taaaaaaatg gcagaagtct tattgcagat
     1801 cacgggtgtg ctccaaacga ttccttcact tgccttatta ggtttactta tcccatttgt
     1861 agggatcgga actgttccag cattgattgc acttgttatt tacgctttgc ttccgatttt
     1921 ccaaaacacg tatattggtc tagctgaaat tgacccgtct attgaagaag cagcggtagc
     1981 atttggtatg tccagaatga gacgcttgct gaaagtagag cttcccatcg cattacctgt
     2041 cattatctca ggtatccgaa ctgccttagt tttaatcatc ggtacggcta ctttggcagc
     2101 attgatcgga gcaggtggat tagggacatt tattttgtta ggtattgacc gaaatacacc
     2161 tgttttgaca ttgattggtg cgatcagttc tgccttgctt gcaattatat tcagcggact
     2221 gattcgctgg ttgcagcata agaaacctcg atattccatc attactttag cagcagtggt
     2281 gtgtttattt ggcggcgttt ctttatatca gtctgccttg tttaaaaatg aaaccatcac
     2341 gattgctgga aaattaggag cggaacctga aatcctgatc caaatgtata aagaattgat
     2401 tgaagacaac acaaaaacag aagttgaatt aaaaccgaat tttggcaaga cgagtttttt
     2461 gttcaatgca ctggatcaag gtcaaatcga tatctatcct gagttcacag gtactgtcct
     2521 agaaagcttg gtgaaggtac ctgaagcaat gaaaagtgct gatttgacaa aagaaaaaac
     2581 atatgacgaa gcagaagaat tattgaataa gcaattcgat atgacattat taaaaccaat
     2641 ggcttaccaa aatacgtatg ccttggcagt caagagatct tttgcgaacg aacatcaact
     2701 taaaaagatt tccgatttgg cagctatctc tgatcagatc aaagcgggat tcacactgga
     2761 atttattgat cgttcagatg gatataaagg aatccaagaa gcatatggct tgaatttttc
     2821 aagtgtacaa agcatggagc caagtttgcg ttaccaagca atcaataatg gagatgtcaa
     2881 tattgtggat gcctattcca cagatagcca gctcaaggag tacgatctag tgactctaga
     2941 agatgatcga gggttattcc cagcttatca aggcgcaccg ctgatgaaaa aagattttgc
     3001 taaagaacat cctgaaatcg ttaaagcact gaataaatta agtggaaaaa taacagagga
     3061 tcaaatgatt gatatgaatt accaagtcaa tgtcgaaaag aaaaagccga cagaagtagc
     3121 acgaatcttc ctgaaaaatg aaggattagt aaaggaggaa gatcagtgac taaattaatc
     3181 gagttcaaac atgtgcaaaa aaagtatgat ggaaagtatg tcatcgatga cctaaatctg
     3241 gcaatcaaaa aaggagaaat ctttgttttg gttggtcctt ccggtagtgg aaaaacaact
     3301 actttgaaaa tgatcaatgg attatctaaa ccatcagcag gagacatcta cttcaaagga
     3361 aaatcgttaa atgaatataa tctgcagaaa atgcggtgga atatgggata tgtgctgcag
     3421 caaatcgctt tgttcccgac catgacagtc aagcaaaata ttgaagtcat ccctgaaatg
     3481 ttaggttggg aaaagcagaa aagagcagac cgtgtagatg aacttcttca aaaagttggc
     3541 ttatctcctg atatttatcg agatcgtatg cctagagagt tgtctggagg agaacagcaa
     3601 cggatcggga tcatccgagc tattgctgca agcccagatg tgattttgat ggacgagcca
     3661 tttagcgcat tagatccgat ttcaagaaac tcattacaag aattggtttt gtccttacac
     3721 gaagaattgg gcacgaccat cgttttcgtt acgcacaata tggaagaagc aatcaagcta
     3781 ggtgatcgta ttgcatttat gaaggatgga gaaattatcc aatgtgacac accagagcag
     3841 ttattgatga atcctaaaaa cgactacgtt cggcatttct ttgatgaacc gaaacagacg
     3901 aaagagtggc gagtagaaga tttagtcgtc aatggatatt tcttaaatga aataccagaa
     3961 aatgctcgtc aacaagtttg ctttgatacg cctatgaaag aagtatattc gcttctttcg
     4021 gtgtatccaa gcattgtgat agtagaaaag caaagaagta ttgggtcgct gacaagtaaa
     4081 gaaatctttg cttttttaag tcgagaggag gaaggaaatg aaaacatatg atgcaatcat
     4141 catcggttca ggtgtgagcg gcttaagtgc tgcttatgga ttgaaagaag caggtaaaac
     4201 agtactagtg gtcgaagaag atctatgggg cgggacttgt cctaaccgtg gatgtgatcc
     4261 taaaaaagtc ttgttaagtg cagtagaggc acgtaatcgg gtaaaacaat tgagtggaaa
     4321 aggttttaac gagatcccaa ctgccaactg ggaagaactt caaaaattca aacgaacatt
     4381 tacggatcca gtcccagaaa gccgaaaaaa acaattagca gaagctgaaa tcgatcatct
     4441 ttcaggaacc gctcgttttt tggatgattc atctattgaa gtcaacgaag aagtcttcca
     4501 tgcggattac ttagttttgg caaccgggca aagacctacg atcttaccag tggagggaaa
     4561 ggaatacttg aaaactagtg cagacttttt atcattgcct gtactgccta aagaaatcat
     4621 ttttatcggt ggtggttata ttgcttttga gctagctacg attgccaatg ctgcagggag
     4681 caaagtaacc attgtccatc ataatcaacg gccattaaaa gaatttgagg cgtctttggt
     4741 agaggaagcc gtccatcaaa tggaagcatc tggcattcag tttgcttttg gtgttgaaac
     4801 ccaaaagatc atttcagaag gtactcgcta tcgtttagtc ggaaaagaga cggaattagt
     4861 agcagatatg attttttgtg cgactgggag acagccgaat acagaatctt tagcacttga
     4921 gcaagcgaat atcgtctttg ataaacacgg aattgcagtc aatgattatc tgcaaacgag
     4981 caatccaaaa atttttgcgt gtggagatat tgtttcaagg aaaaccccaa aattgacacc
     5041 agtcgccact tttgaaggaa attatgtagc taagcggata acggatgcaa caagcgaacc
     5101 tatcaaatat ccgattatac ctacgatcgt ctacgccagt cctaaacttg ctgaagtagg
     5161 agttacaaaa agtcacgcct cttcgtcaga tcaagtggta gagatggatc ttaccagttg
     5221 gttcacctat catcgtgtca atgaaccagt tgctaaggct gaattgacct ttgaccagca
     5281 aaattatttg attggtgctg cagtgattag cgagcaagca gacgaattga tcgatgattt
     5341 gacactcgtc atcaatcaaa aattgactaa aaaagagttg gatagctata tcatggggta
     5401 tccgacattg gcaagtgatc tctcctattt attgaaataa tcagcatgat aactaaagcg
     5461 cttgttgctt tttccttctt gtgctacact ttaaaaaatg aatacctgtt tctggggatg
     5521 tcgtagacga catgagagta agttgtgaac ttagacccat tgaacctgtc agttaatact
     5581 ggtgaaggga gaaacatagc agacgcttga agcgggttac tatgtcgtgc ccatttcaag
     5641 cgttttttgt tattcatatt agaagaaagg aggtaacaaa atgaagaaaa atctttatcc
     5701 agcagtcagt atggcattca ttttaattat ttggcagatt gttgtcatag tttggaaaat
     5761 acctgccttt attttacccg gacctgctgc tgtattttct gcattagcgg cagatgcaca
     5821 agtattactt tcccattctg tcacgacatt aaatgaagca gtgcttggtt tggtaatagc
     5881 tagcgttctt tcttttatta ctgccttggc aatggattat tggaagtttt tagaaatgtc
     5941 tttttatccg ttactagtga tttcacaaat gttgccaatc atggtgttag ggccattact
     6001 taccctatgg tttggtttcg gtatgcttcc aaaagtgatc cttgttgtct tgataagcta
     6061 ttttccgatc gtagtttctt tttcggatac attgaggaaa gtatcaaaag agcaaatcat
     6121 ctttttgaaa acgatggggg cagatacagg gaagatttat cgaatatata aaataccagc
     6181 gggtttatcc ggcttctttt ccggtttaaa agtggctgct acttattgtg tcagcggagc
     6241 agtcgttgga gaatggctaa gcgctcaagc aggcttaggg tattacatga tacgcgtaaa
     6301 aaacagctac cagatcgata aagtttttgc tgcgatcatt tgtgtcattc tattgagttt
     6361 attgctgaat ggcgcttgtt cattattgaa aaaagggtat tatcgtatgc tttatatttt
     6421 ttaagagaag gggatcaaat gaaaaaatta tggcttttat taccgctact gttgctttta
     6481 tccgcttgtg gcactgcaaa agaaacaagt agcaaacaag aaataaagga tttgaaaaaa
     6541 gtgacacttg ttttggatta tgtaccgaat actaatcata ctggcatcta cctagcaaaa
     6601 gaaaaaggct attataaaga ggctggttta aatgtccaaa tcatcgaacc aggagataat
     6661 tcgacaagta ttggtctagt aggtgcggac aaagcacaat ttggtgtaag ttatcaagaa
     6721 gatgtcactt atgctcacgc agatggacag aatataccag tcaaagcaat tgctacagtg
     6781 atcaaacata atacttctgg attcgccacc ctatcagaca gtaacatcca ttcaccaaaa
     6841 gattttgaag gaaaaacata tgcaggttgg caaagtccaa gtgaagaagc tgtcttaaaa
     6901 gctgtgatgg aaaaagacgg cggggacttt tcaaaactga caatggtagg aagcaatgga
     6961 gaagggccag aaagcttagg aaaatcctca gatatccaat ggtactttga aggttgggat
     7021 atgatcaaag caaaagaagc gggtattgaa ctaaactata tcccattgaa agaattagat
     7081 gaacggttag attactatac tccggtaatt attacgaatg atcaactgat caaaagtgat
     7141 ccagaattgg tacagtcttt tatggatgcc acgaaaaaag gctatcagga agctattaag
     7201 gatccaaatg acagtgctaa actgttgcaa aaatatgcaa aagaaaatga tcggacgttt
     7261 ttagaagaat cacaagcatt cttgtcaaaa aattatacgg atgatcctga gaattgggga
     7321 ttgatggaag aaaaagtttg gcgcaattat acagctttta tgcaggaaaa cggattgatc
     7381 aagcaagatg taccttcgca agaactattc actaatcagt ttataaaata ggagtgatag
     7441 atatgtcaat ccaaatcagc cacttgaatg tagtcattga aaacctgaaa atattagaag
     7501 acatccattt cactatccct aaagatacct ttacatcgat tgtcgcgcca agcggagccg
     7561 gtaagtcaac attgctgaaa actttaacag gagtccagcc tatgacttct ggaacgatca
     7621 aagtagacga tcaaaaagta actggtctcc atacagcatt tagttatatg ccgcaagaag
     7681 atatgttact tccttggctg agcgtctatc aaaacgttac gttatatcaa aaaatcaatc
     7741 atctaacaat caatgaagaa caagtaaggg attatctgga tatctttggg ttaaacggct
     7801 atgaacattt tttgccggaa caattacctg gcgggatgaa gcagcgaacg gccttgttac
     7861 gaacaatcat gaatccttct tcttacttgc ttcttgatga accatttgga gcgttagatg
     7921 ctatgaccag agggcagatg caagactgga tgttgaagct gcctaaaaaa gcaaagcgga
     7981 ctactttact tgtgacgcat gatatcgaag aagcaattta tttatcagac aggattcttg
     8041 tgctttccgc ccgtccggca catgtgattg ctgagattca agtgcctgaa aaacgacggt
     8101 cacgtgaatg gctactgcag caatcagagt tgaagcaaac gatttatcag ttattggcag
     8161 gtgaattaga tgttaagtga tgctcattgt catttagatg ggagtcagag tttagcactc
     8221 cttcagcagg aacacacgat tttgacgatc atcaattgtg attcgccaga agaatggaag
     8281 gaaaacagac agcttgcagc aagtaagact caagccttaa gttacggtat ccatccttgg
     8341 aaagcagata gctatacgtt tgaacaagtt gagccttttt tgaaaaaagc aaggatcatc
     8401 ggtgaaatag ggttagataa tatttggacg aatgtaccga tgactacaca aaaaaaagta
     8461 tttgagcgac agcttgcttt tgcagccata aatgagaaac ctgttgtact gcacacaaaa
     8521 ggatgcgaaa aagagatctt aaattatatc caagcttacc ctaatcacta tttgatccat
     8581 tggtattctt ctttggacta tcaacaagac tatattgatt taggctgtta tttcagcatt
     8641 ggtcttgatt tgaaaaagaa tccagcagtc tggcaactag ctaaagcagt cccaatcgac
     8701 cacttactta ttgaaacgga tggactacat gcagcagagt gggctttagg ggggattttt
     8761 tcagaaaaag attatgtctc actgcttgaa gaacagttaa agacaatcgc tgagatcaaa
     8821 gagctatcag ttgaagaagt taaaaaaaat acttatcaaa acttggaacg tttcattaga
     8881 ttaggtaaat aaaaaagaga tgtgacacaa ctagtgtcgc acctcttttt ggctttcttt
     8941 gtataaaagt aaactgtttg attaataaat gaatcttagt accaaccgtt tgcttcccag
     9001 aatgctttcg ccgcttccca agaaccataa cgtgaagtaa catattgttc tgctactctt
     9061 tcttggtttg cagcagagta gtcaccattt aagtaagaag aatctaattg gtaacgtccg
     9121 atgtaacgtc cgttcgttgc tgtgtaagaa ccgcttgatt ctttttgtgc gatccattct
     9181 tttgcagagc ttgtgcttgc tggagtagca gcagtttctg ttacagcagg agcaggttgt
     9241 gtttcagcaa caggcgctgc ttgtttttcc gtttgaaccg tttcttgtac aacaggttgt
     9301 tcagcttgga ctggtgcttc ttgaacggct gcttgaggtt cagcagctgc ttgtgcagag
     9361 ccttctgttg ggattgttaa ttgttgtcct gagta
//
LOCUS       DAZKKW010000096         9248 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.098, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000096 DAZKKW010000000
VERSION     DAZKKW010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9248
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.098"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..389)
                     /gene="comGD"
                     /locus_tag="ACG8PO_002335"
     CDS             complement(<1..389)
                     /gene="comGD"
                     /locus_tag="ACG8PO_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGD"
                     /protein_id="HFQ0207623.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAY"
     gene            complement(364..642)
                     /gene="comGC"
                     /locus_tag="ACG8PO_002336"
     CDS             complement(364..642)
                     /gene="comGC"
                     /locus_tag="ACG8PO_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus major pilin ComGC"
                     /protein_id="HFQ0207624.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            complement(639..1688)
                     /gene="comGB"
                     /locus_tag="ACG8PO_002337"
     CDS             complement(639..1688)
                     /gene="comGB"
                     /locus_tag="ACG8PO_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus assembly protein ComGB"
                     /protein_id="HFQ0207625.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            complement(1645..2574)
                     /gene="comGA"
                     /locus_tag="ACG8PO_002338"
     CDS             complement(1645..2574)
                     /gene="comGA"
                     /locus_tag="ACG8PO_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus ATPase ComGA"
                     /protein_id="HFQ0207626.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLKLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            2706..4460
                     /gene="ade"
                     /locus_tag="ACG8PO_002339"
     CDS             2706..4460
                     /gene="ade"
                     /locus_tag="ACG8PO_002339"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006146 - adenine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HFQ0207627.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            complement(4558..5220)
                     /gene="trmB"
                     /locus_tag="ACG8PO_002340"
     CDS             complement(4558..5220)
                     /gene="trmB"
                     /locus_tag="ACG8PO_002340"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFQ0207628.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(5290..6072)
                     /locus_tag="ACG8PO_002341"
     CDS             complement(5290..6072)
                     /locus_tag="ACG8PO_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HFQ0207629.1"
                     /translation="MMEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAA
                     LSREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLK
                     TMLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHG
                     DVNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDE
                     MLEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            complement(6276..7487)
                     /locus_tag="ACG8PO_002342"
     CDS             complement(6276..7487)
                     /locus_tag="ACG8PO_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0207630.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            complement(7480..8217)
                     /locus_tag="ACG8PO_002343"
     CDS             complement(7480..8217)
                     /locus_tag="ACG8PO_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0207631.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            8355..8789
                     /locus_tag="ACG8PO_002344"
     CDS             8355..8789
                     /locus_tag="ACG8PO_002344"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HFQ0207632.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            8782..9126
                     /locus_tag="ACG8PO_002345"
     CDS             8782..9126
                     /locus_tag="ACG8PO_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HFQ0207633.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
BASE COUNT         2940 a         1912 c         1591 g         2805 t
ORIGIN      
        1 tgataagctt ttagtgaact ttcattcccg gttctagcag caaaagtaat agaagcatgg
       61 cgtttcaagg taatctcatc aggaatatct aatacttccc aatttatttt atctggattc
      121 ggaacatgaa aaatgatttg attctcttca ctattccaat aaaagcctgt ttggatctga
      181 ttcacaatag cgattttttg cgtagaatag attcgttttt caaattggcc gaaaaattga
      241 tagactgcca attcattctt ccatgcagaa aatgcgagaa ctggaaaaga aaggatcgtt
      301 gtaagtaata aaatcagtaa gagtgattca aacaacgtat atcctgatgc taaaacctta
      361 ccttcaaggc cgctgataga tttctgcatg ttctcgtttg atatagcctt cattcaacaa
      421 ttcctccaca gtaggaactc ttccattatt ttgaagcgca tagagctctt tttgtgtgtc
      481 taccaactgg atgatcgctt cacgactttc ttggtctaca tgattcctat aacgcgacaa
      541 attagggacg aacaacaaca ctaatacact gatgatcagt aagacgatta gcatttcgat
      601 caatgtaaaa ccgctataat aagatatttt tctctttttc atggcaaaac ctcctctatg
      661 cttccgtaaa caggtaataa aacagcggca taaactaata aaatcaaagc cgctacgccc
      721 aaaaaaatga ttggctgaat aaaatgcatc cagtgatgaa gacgtttgaa aagctgtttt
      781 cttgtccatt cgctgtaaaa aatcagttct ttacctaaac ttccctttgc ctctccctgt
      841 aaaacaattt tactgaattc ttctgataga aaacgataat tctggaactg ctcagccaat
      901 gacatacctg cctccaatcc ttgaatcaac ttagaggcaa gatgctgaat cagactttct
      961 tgtttcgttt cctttaagca gactatgatt tgccttaatt cgaaaccttc atgaaaaagc
     1021 tttccaatct ctagtgacaa ataagccgat tgatacaagg aatatatcgg tccaatcaat
     1081 aattgtctgc ttatccattc actctgatca acaggagaca acttcatcag acgatacctt
     1141 actagtaaag ccaatagagt aaccgctagg atgaatagta gaacatacca atgaaagact
     1201 tgtatcagct gaatacccca atgctcttgc atgaccattc ctgaaccaat aagctgaggc
     1261 aaaacgaaca accgcatact aaacaagata cctagcaaaa aggtgagcaa tagcatcgga
     1321 tatgcaagca gctttttcaa atcattgcga aatccttcaa caagccgaaa ctgtttggca
     1381 atttccaaca aagttgtcgg cagattccca tgacgttcag ctaactcgat ctgtaccagt
     1441 tgctctgctc catatcctat ttgcgaaagt acctcactaa acgcttgccc ttcctttagc
     1501 tgatgttgga tatttgccaa aataacaggt ggaaacgaac ggatcgtgag taataaataa
     1561 agggattctt gcagactaaa accagataaa agcagttctc ccatggtttg tgcaaactgg
     1621 ttttgctggc ttcttgttaa tcctttagaa tagttctgac tgccaaatat tttcctgagc
     1681 ttcttcataa ctttcctccc atgatttttt tactgattct tcctccataa attgataact
     1741 ccataaaaca gaaggatggt gattggcatc ttccaaaagt tcttgataaa tgacaccagc
     1801 taaacattca gatagctctt cttttttctt tgtcagttca tggattctga ccaatgtacc
     1861 ttcaagattg cgtgcatgta cggttgcaaa aacccggtgc cctgttagtg ctgctcttac
     1921 cgcagcatgt gcagttgatt catctcttat ctcgccaatg attagcaagt ctggacgatg
     1981 ccgtaatgcg agtttcaaaa gtgtatcata agtctgtgct attttcggat tgatctgtag
     2041 ctgcagaaaa aacggttctt ctatctctac aggatcttcg atagtaatca cttgcaattt
     2101 ttcttccaaa gccaaccgat acattagaga agtcttgcct gatccgactg gtccgctgaa
     2161 aaggtataat ccacgtctga cggttttctg taaaatagtt gcaaaatctc gagaactaaa
     2221 gcaacgatgg ggtgtttcaa aagtaccgta taatagccga atcactaaac tctctcgttg
     2281 aaggtaatct cctacagaag acaaccgcag acgaacttcc tgtttttcta aagaataagt
     2341 aaatgcacct aactgagctt ttcttttttc tccgatatcc atctgtccca aatacttaaa
     2401 tcttgagatc agttgctgtc catctgccga agagactttc ttccagaatg ttcgtttttt
     2461 tccatagcga aaatagatgt aaaatacttc tgaagctggc aatatatata cgtcttgggc
     2521 atgtttggca aaactttcac ttatcatttg atccgataat cgttttattt ccattattct
     2581 cccctccaca aaaaacatac gtctttttga tctttttgtt tcaaataaaa tgaatttaca
     2641 aaaaaatatt ggcaaaagcc gaatttatgt tatacttata aaaatttatc ttgggaggca
     2701 ctccattgga aacaacatta aggaaatata tcgaagtagc aagtaaaaaa aagcctgctg
     2761 acctagtgat caagcatgct aagatcgtca acgtctttac gaaagaaatc atggaaaaag
     2821 atgtagcaat ctgcgaagga atgatcgtag gaatcggtga atacgaagga aaaagcgtac
     2881 ataatgctaa tgggcaatat ctagtgccag gattcatcga tggccatgtg catatcgaaa
     2941 gctcactttt atcaccaagc gaattttcaa aagtatcctt gcttcatgga gttactactg
     3001 tagtgacaga tccccatgaa ataggaaaca ttgctgggag tacaggtctt gagtttatga
     3061 tagaagatgc aagacaaaca ctcatgaata tcttttttat gctaccttct tgtgtacctg
     3121 taaccccttt tgagaccaat ggtgcaaatt taaatgcaaa aaaattagcc ccttttttac
     3181 aaaaacccga agttttaggc ttagctgaag tcatgaacta tcaagcagta gcaaataatg
     3241 aaacagacat cctagaaaag atccaattga tgcaccagca taagaaaaaa attgacgggc
     3301 atgcagctgg tatcggcatg gaagagttaa atgtttatcc tgcagcaggt attcggacgg
     3361 atcatgaagc tacaactgca aaagaagcta aagaacgttt ggatcttggt atgtatttaa
     3421 tggttcgaga agggaccgtt gcaaaagatc tggcatcgct actaccggcg atcacacctg
     3481 aaaattcaag aagatgcttc tttgtcaccg atgataaact gatcaatgat ttagtaaaag
     3541 aaggatcaat cgatcatatc gtccgtcaag ctattgcact tggaatcgat ccattacaag
     3601 ccattcagat ggcaacattg aatgcagcag aatgttttga tttgaaaaca ctaggtgcga
     3661 ttgcccctgg gtatcaagct gatttcttct taacagacga tcttaccaag ctgccaattc
     3721 atgaagtttt ctataaggga acattagttg ttcaatctgg gcaactccaa gaccatttat
     3781 ttgctgatca ttcaaattca tatacgtgtt gtctgccaaa actaaacgct caaccattaa
     3841 atccacattc gttagagctg cttctttctt cttcacaagc ccatgtgatc gaaattatcc
     3901 cgaatagtct agtgactaaa gatttggtag aaaaagtgga ccgacaaggc gaacgattca
     3961 ttccttcaat cgaaaaagac caattaaaga ttgcggtgat cgagcgtcac catcaaacgg
     4021 gaaacattgg attaggcata gtgaaaggat ttcagctgaa acaaggagcg atagcgacaa
     4081 cgattgccca tgactcgcat aatctagttg tcgttgggac aaacgacgag gatatgctgt
     4141 atgctgctaa tcttttgatt caaaaaggtg gcgggatgac cgttgtcaac aaacagcagg
     4201 aaatagcttg tgtttctctt cctataggcg gaatcatgac acaggaacct ttttcgatcg
     4261 tctatgaaca gttgaatgaa ttaataacta aagcacatca actaggagca gctaaaacct
     4321 ttgatccttt tttgacactt tctttcttaa cattatctgt catacccgaa ttgaagatca
     4381 ccgacaaagg attattttca ttttctcgct tccaattgat tccaccttgt ggaaaaaccg
     4441 atgaaataaa tacattttaa atcaaataaa tcccagaaaa tacattacat ctgtaatgtt
     4501 cattttctga gatttattta tggatattaa tgtttttatg aaagcagaaa gttagtttca
     4561 cagacttttc tttttggtct tatcttgaaa ttgagcttct actcgataaa tacgttgacc
     4621 ttttgaagaa aatttttctt catactccgt cataatattg ccttcaaatt gactttgatg
     4681 taaatctaac catacctgtt tgaggatcat gccatattga gaaaaacttg ccagagaata
     4741 ctcaaacaat ccttgattat ctgttttgaa atggatttct ccatttggac gaagaatctg
     4801 ttcatctaca gcaagaaaag tcttatatgt caatcgccgt ttttcatgac gtttttttgg
     4861 ccatggatcg gaaaaattca gatagatttg atctacttcg ctgtcagcga aatactctgt
     4921 caacgctgat ccatccacat gtagaagttt caggttagga agcggttgtt cgatcaactt
     4981 gtctagtgca atcgaaacaa cactgacctg catttctact ccaatataat tgatttcagg
     5041 atgggctttt gccattccag tgatgaactg gcctttcccc ataccaatct caatgtggat
     5101 tggatgatcg ttttcaaaca attcattcca ttttcccttc cacaatgttg gatcactaat
     5161 tacaaaattc gggtgagccg ctaacatttc cgctgctccc ggacgatttc ggacacgcat
     5221 tatttatctc cattcctttc ctctaattaa aaaggtctgg ccaaatctcc gcagagtttg
     5281 ggccaaacct cagccactga aaattcgttt taattgtaaa atttcttcgt tcatccgtcg
     5341 atgatcttca cgtttgcaat atctggtgat ttcttggagc aaattcatgg ccgcatacca
     5401 atgaaatttt tcaagcattt cgtcagtcgg gcgtatgcca taactgatca gccactgatt
     5461 ccagctagac agtggcacat aatttcctaa aatcgttgca atatccaccg cagggtctgc
     5521 aaacatgatt gaatcccaat cgactagata taaataattt ttacatacta accaattacg
     5581 atggtttaca tctccgtgta ctactgataa gttttcctta tctattgacg ggatctgctc
     5641 acgaagaaaa cgatggacaa tttgcagaaa ttgatttttc tggagttctt ccggtaattc
     5701 tttttcatat ttttgcagca tcttctccgg cgtcatgaca cgaccaccga tacgggtcag
     5761 catggtttta agagaatttg aatgatgtaa atggtacaaa acatcaatca cgtcgtttct
     5821 ttgaccaatc tcattaggag tcagcaagcg accttccagc cactcttgtg ctgtcaaagt
     5881 atcgccattc cccgttcgtt ttgtccaaac aagttttggt gtaattcctt cccttgaaag
     5941 agcagccaac atcggagtcg tgtttcgttt aataaagact ttgtcctcat cacgcatgcc
     6001 tatgtatgtt tttcctgtgt cgcctttgat gggacgtaac cgccactctt ggtctaactg
     6061 aaactccatc atgcattctc ccgtcctctt actctttgtt ttgcagtact tattattcta
     6121 gtcgcctgaa cactacacgt caagcacata tacgttcttt ttcatttaat tgccattcaa
     6181 aaaaccagct tttctcaaaa aaaatagaac gaacagcatt cttactgtca acgctctatt
     6241 tttcattctc atttttaccc tacacagctt tgttttcagt ctgacatctt cttcaaacga
     6301 gttggtacat atagtttaac aaagagcata gtaacaatca aataagccgc tagcggaacc
     6361 catcgctgct gcccatctaa tacgactgca gcaatcaaac caaaaataac cgacgcgata
     6421 attaataccc atgtgagtag atactgaatc gcctgcttct tctgattctt atggattgga
     6481 taaagctgaa cgagaaccat atagcggaac tggttcgcta aaggtaacag ttggaaaccg
     6541 atcaaatata aaaataagaa accgataatc agactaaaat aaaagtcgac tactccggca
     6601 atcaataacc cacctataat tactaatcgg aaataaagac cactaaaatc agaacctctt
     6661 gccaatcttc ttgcaaacaa gtaaagataa gtgttttttg attctctttt aatatgagtc
     6721 aatataggat ccaaatagcg tcttcttttg atctttgccg taatttcagg aacatctgtg
     6781 aataaattga taaattgata gatctgatgc aacctatgtt cttctacatg caccattttc
     6841 tcccaatcca atcctttttc ttgttgcttc cacaaaaacc agtaatatag acttgcctgt
     6901 actaatgcac ctgctattcc tgcccatatt agccaataaa atgatagaaa caaaaagatc
     6961 agagaagaaa aaaaccagcc taatttccaa taacgaataa cttgttgatc tagtcgaaac
     7021 aattctgctc tcttgatttg cagatgactg aatttcaaac tccataatat gatcagatag
     7081 acgaaaaaca tcgaaaagga ttgctccgta ctaaccacca ctaatggcat ggccaatccg
     7141 catgccaata tcaaaaagat caacggaaaa atgcaagaat agcgaaaagc tcgttccaaa
     7201 taggtttgca tttctttttc tttaggaagt aaaaaaacaa tatctgctgc ctctgcaaaa
     7261 gtagccatct tacccacgaa taaacagagc atccaaaata cagagattat gagccctcca
     7321 attgcaaacg gtggctgcag ggttttcaac caattagaat aatataatcc cacaccgcca
     7381 atcaagaaaa gacaaactaa aacaaaatga tcgttcaata cataacgcat atatttcatc
     7441 attttctttt gatgtcttgc taaacgtttt tgaaaaaaac tactcatgtg cctgctcctt
     7501 tgttaaagca atatagattt cgtccaaaga agaatccggc agctgaaatt cttttctcaa
     7561 catctcaaga gaaccgtctg ctcgtatctc tccgttatgt aagaccacaa aacgatcaca
     7621 atatttttct gcagtagcta aaatatgcgt agacatcaaa ataccggctc cttctttttt
     7681 catttctacc atcaattcaa gcaatgcatt gatagctagt ggatccaagc ctaaaaatgg
     7741 ttcgtcgatg atatataaag aaggctcgat caaaaaggcg cataagatca tcactttctg
     7801 tttcattccc tttgaaaaat tagctgggaa ccagtcaaga cgattagata accgaaaagt
     7861 ctccaacaac ttttctgctc ttttcattgc tatatccata ggtatatcgt aggccatcgc
     7921 agtgacttcg atatgttctc tcaatgtcaa ttcttcatat aaagaaggag tctctggaat
     7981 atacccaatt tgtttgcgat attcttccgt atttttcttt aaggatagat gattgatttc
     8041 tatcgttcct tttgaagctt ccagcagacc gataatgtgc ttgatggtcg tacttttccc
     8101 tgctccgttc agtccaatca accctactat ctctccaggc tgtacatcga aactgatatc
     8161 ttttaacaca gggagatgtc cgtacccccc tgtgacatga tcgataatta aactcattct
     8221 ttttcctccg ccatttattt ttatttatta taccacgaag aaaatgacgg tattttgttt
     8281 ttttccttac aatagcttta acttatggta aagttaagct gaagtattta ttctttatag
     8341 aaaggatgtt ttatatggaa aactgtattt tttgcaaaat catcaatcaa gaaatcccaa
     8401 gctataaaat atatgaagac gacaaggtct atgctttttt agacatctct caagcaacga
     8461 aaggacacac attagttgtg cctaaacgtc atgtagcaga tatttttgaa tatgatcccg
     8521 aacttgcagg agaggttttt tcgcgtgtgc ctaagattgc tcaagcattg gaaaaagctt
     8581 ttccagaaat gaaaggtctt aatatcttga ataacaaccg tgaactagcc tatcaatctg
     8641 tttttcattc acatatccat ttagtccctc gctacagcaa agaggatgat ttttctattc
     8701 actttgtcaa tcatcaagat agttatggat cggaagaact aaaagctatt caagaaacaa
     8761 tcgtaaagca ggtgtcctgc gatgattaaa aactttttca aaggactgtt atttggagct
     8821 gctgctggca ctgtcggagg tctgttattg gcgcccagga gcgggaatga aacaagaaag
     8881 aaactgatcg aagaattgga agaagcaaca actctgacaa atgatttaaa taacagcctg
     8941 aatcatttta aaagcgcatt gatcgaaaca aaagaaacag ctgaatctgt gattcctgct
     9001 ttccaagaag caatcaaaaa agatatcgaa gattttaaat tccaagccga accacgtatc
     9061 acacaaatca atgaacaagt agaaaaacta aaagctgatc tgccagagta tcctgaacaa
     9121 atataataat gattattttc tcaacagatt taactatata agtgagaaaa gtttgcgtat
     9181 ttcctaggaa tgtgatgaaa ttttgaattt tgtttgaaac taccacgaca acgtttttaa
     9241 aggaaatg
//
LOCUS       DAZKKW010000097         9027 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.099, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000097 DAZKKW010000000
VERSION     DAZKKW010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9027
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.099"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..488)
                     /locus_tag="ACG8PO_002346"
     CDS             complement(<1..488)
                     /locus_tag="ACG8PO_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0207634.1"
                     /translation="MKYILCQPAINRFKWELEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFFEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDDRYFYIDSDVIFNK
                     RINLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVNVTVESLETINTN
                     SGG"
     gene            complement(498..1658)
                     /locus_tag="ACG8PO_002347"
     CDS             complement(498..1658)
                     /locus_tag="ACG8PO_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFQ0207635.1"
                     /translation="MTIKNLKGATAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNGFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKKAEPITALDAVTMGGLMARLSKFNIEGVNDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTQA"
     gene            complement(1658..2344)
                     /locus_tag="ACG8PO_002348"
     CDS             complement(1658..2344)
                     /locus_tag="ACG8PO_002348"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0046797 - viral procapsid maturation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head maturation protease, ClpP-related"
                     /protein_id="HFQ0207636.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSSGTEAKKEQSVEEILNDVKEKRAEKIAAFTAAL
                     NKTFGQGDAK"
     gene            complement(2307..3485)
                     /locus_tag="ACG8PO_002349"
     CDS             complement(2307..3485)
                     /locus_tag="ACG8PO_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFQ0207637.1"
                     /translation="MGIFQKAVGYFTKKATVPLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRPQDNTTQDQINKMMTSQFKAFMEA
                     DNAGAVFQLQNEYTLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGVFTINEGRERVGNDRSDKAMADEIFITKNNQQVSKGGEANDDNENISSSKE
                     "
     gene            complement(3505..5199)
                     /locus_tag="ACG8PO_002350"
     CDS             complement(3505..5199)
                     /locus_tag="ACG8PO_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFQ0207638.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDVFKVIKDHPDLDKKVFK
                     ATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEIHENEDYSMINVATSGGGKIR
                     DYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKDGAEFSSLFPFICRLDNDNEV
                     DDPDMWEKACPTINYNADLKRKMFQEYSQMQRNAGLRLTFMTKRMNRPMEDTRFAVAS
                     YDDVLHTKEKEFPEKMDEVIGTVDFADRRDFASVGLLGKYDKDVYFTQHTFIHESALR
                     LQNIKREVIDISIDQGKSQIVHGKNIEADYIVGWFLEMSNKYYIKKIAMDMYRAKILK
                     PALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQRLFFGDDAIMRWYCMNVYEEHISN
                     GNIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSAPVTVTNSSVENTGTGFTPLVF"
     gene            complement(5177..5491)
                     /locus_tag="ACG8PO_002351"
     CDS             complement(5177..5491)
                     /locus_tag="ACG8PO_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P27 family phage terminase small subunit"
                     /protein_id="HFQ0207639.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
     gene            complement(5594..5875)
                     /locus_tag="ACG8PO_002352"
     CDS             complement(5594..5875)
                     /locus_tag="ACG8PO_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0207640.1"
                     /translation="MIINDNGREYDTEKIEEYSSYTQGLIKRLIYVRYVGIRDLLSDN
                     CCSKYKVNQVREALNKDNNVERIKNVFGYSIEEINYYIDFAEAFIPMVR"
     gene            complement(5880..6224)
                     /locus_tag="ACG8PO_002353"
     CDS             complement(5880..6224)
                     /locus_tag="ACG8PO_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFQ0207641.1"
                     /translation="MTEEFYRWLLQLIREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
     gene            complement(6250..6417)
                     /locus_tag="ACG8PO_002354"
     CDS             complement(6250..6417)
                     /locus_tag="ACG8PO_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0207642.1"
                     /translation="MYRPQYLEQKHEVITVQNGNGEIVRKYRRPIKSDTYKRKESNEV
                     IPLYGKRIAKY"
     gene            complement(6484..7131)
                     /locus_tag="ACG8PO_002355"
     CDS             complement(6484..7131)
                     /locus_tag="ACG8PO_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0207643.1"
                     /translation="MVGVVKFLGFTFDGYGTLGEWVGIILIVITIQQTSKYYWYSNSS
                     KIVSRITEGITKKDITVVGKYGFTITLLNEGKSETAIKFCGIIGKYNIFQRFIRKHWY
                     KYIYKYPVVKKLIEKYVDHEISYLNPTDLINQEFQNIGYLEKGLKIPIVNESLVTAMV
                     ANIKNSKKLEKRYKKCKKLKFSFLFLEYNGKEYYQDIYVLRGSNLYKDIKNKISV"
     gene            7341..7532
                     /locus_tag="ACG8PO_002356"
     CDS             7341..7532
                     /locus_tag="ACG8PO_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HFQ0207644.1"
                     /translation="MVKNLYFVIREATNGQYYFVIKSNNNEVVATSETYLTKYSAEQT
                     INSIKNGITKDSQVIDMTK"
     gene            complement(7525..7866)
                     /locus_tag="ACG8PO_002357"
     CDS             complement(7525..7866)
                     /locus_tag="ACG8PO_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0207645.1"
                     /translation="MEVEQIKYQGMDRERLIDIIEQQRLELIQKEAVCKEYKKHLEQV
                     IEYHSVEKYRSVVQKNRATSPDSRQDVSISIKTPVFNSVKSEKLESISTALVEKAAEM
                     NSLANEVSRLI"
     gene            complement(7890..8105)
                     /locus_tag="ACG8PO_002358"
     CDS             complement(7890..8105)
                     /locus_tag="ACG8PO_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0207646.1"
                     /translation="MKIASEKIDQLTILVNQRIEDIIKEFEGEKIKNIKLTLMDHKFE
                     LNSTKRKIVELIEEEIALQINEQQLAN"
     gene            complement(8401..8868)
                     /locus_tag="ACG8PO_002359"
     CDS             complement(8401..8868)
                     /locus_tag="ACG8PO_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family transcriptional regulator"
                     /protein_id="HFQ0207647.1"
                     /translation="MVLFDVKKYETPSAKDVDMERTKHNVAVFLSAYLSARCRVGQPR
                     EPKVTASYSLVPPSTADHIFEAERMLIDKEEAQEEFEYLHKLFVRGYSAIQHPHKPDV
                     TERRKKIFYDRYINGLPIYVTAQRNNTSEESVKVESNRIIIQFASSLELVAFK"
BASE COUNT         2652 a         1841 c         1276 g         3258 t
ORIGIN      
        1 cctcctgaat tagtgtttat agtttccaaa gattctactg taacatttac aatgtttgcc
       61 atatctttta gaatattttc tccgttttca cagcttctaa tataatcaag acttagataa
      121 ctacagcagt cgctacaata ccaaacatca tctttagaag gcaatttgcg caaattaatt
      181 cttttattga aaatgacatc cgaatcgata tagaaatatc ggtcgtcctc acgcgaatga
      241 tcttcttcta aatatttcca ccataaatat ggtttaatcg aaggaatata ctctttgtcg
      301 tcccgcagat catcgtacac atgaacttca acaccatatt ccttctcaaa aaaaatagga
      361 atctgatcat cgtgtctgct gaaaagcaat acgatatctt tgattcctag tttcttcaga
      421 ttagttaaac aaacttcaag ctcccattta aaccgattga ttgccggctg acaaagaata
      481 tacttcattc tgatcaccta cgcttgtgtt gtagttgttg tggttgttgg ttttgtagtt
      541 gtagtagtag ttcccaaagc gctaatatct aatacaatga aactatcgtt acgtttaggt
      601 tgaccgtttg catattgttt agctagataa atgcgttcgt cttcaacaaa atggtattca
      661 tctgaagctt caatttttag tgtagatcct acacccatga agtaatctga ggctacccca
      721 ataactgctt ttccttctgg cacagccgtt gactgcaaat ctgaaactgg tactggcaat
      781 acttgtacgt attctccatt agcagttagt acagtcttag ctgggaatac tttagaccag
      841 taatcagttg gattcacaat taggaccaca tcagaaggat tcacattacg ataaatcgga
      901 tcattcacac cttcgatatt gaattttgat agtcgcgcca tcaaaccgcc catagttaca
      961 gcatctaaag ctgtaatagg ttctgctttt ttttcagcat attctccgct agtttgtttg
     1021 ctcatgtcac gcatcattcc gactggcata tctttaccag taccatcaac aattgcttgt
     1081 tctaatgcaa ttctcaatga ttctactaaa acagtacgga cataacgatc taaccatact
     1141 ggacctaaat caagcattgc cttacataca ggaatataac ctgatagctt gaactgcttc
     1201 atgttaatta catcaaagcc attatctaaa acttttttaa cagcttcgca aagtttaccc
     1261 caccatgctg gattgactcc acgtgacaca atccattctg ttacaccagt tgtgttaaca
     1321 aaagtaattt tttgcaatag tggatgagat tgttctaaat cttcaaatac acgttcaaat
     1381 acagtagctg gcactaattc ttcgacccct gcaaaacctt cgtttttcac tacttcgtta
     1441 tagaattttg tttcttgtgt agttaataca cgctgaccac ggttcattaa tactaattga
     1501 tcttgatttt ttgctgttgc ttcttctaaa attttatcct gaatttcctt agataagctt
     1561 accatagctg cgctaaaaga ttcttcgtta ccatctttaa aagctttcat caattggtcg
     1621 cttgcagctg ttgcaccttt taaattttta attgtcatta ttttgcatct ccttgtccaa
     1681 atgttttatt taatgctgct gtaaatgcag caattttttc tgctcttttt tctttaacgt
     1741 cattcaaaat ttcttcaacg ctttgttctt ttttagcttc agtacctgag ctattttctg
     1801 catcgataat ttcatcgacc aatccataac tcaaagctgt ttctgcatcc ataaacgatt
     1861 ctttttcaag aagttcttgc aatgcttcat ctgtgccatt gaatcgtgtt ttatatgaag
     1921 cctttaccga tttatcaatt gattccagtt ggtcagcaat cgtacggaag tcatcgacat
     1981 ttccttctcc gtatgtggaa gcgcggtgaa tcatcaattg tgcattgttg tagattttta
     2041 tagtatcgcc agccattgcg ataattgaag cagcactagc ggctaagccg ttaatcacaa
     2101 cgttaacttt tgctttattt gacttaagta agttcccaat agcaatccct tgaaatacgt
     2161 ctccaccgtt tgaattaatt actacttcaa tttcttcttg atcacctaga ctatccaaaa
     2221 tatttttgat tcccttgtca gtattccctt caaagaacca actagaacca ataaatccct
     2281 gaataaaaat ttgcggtact gcgccttcat tctttactgc tagaaatgtt ttcattgtcg
     2341 tcattcgcct cacctccttt cgatacttgt tgattgtttt tagttataaa tatttcatct
     2401 gccatcgcct tatcagagcg atcatttcca acgcgctctc ttccttcgtt gattgtaaat
     2461 actccatttc taatgcctac atcaatagcg tcaaccaaat ctttgaagct agtaatcttg
     2521 atcatagttg tatccacacg tacaaaattc cctgacaagt attcttctac ttcatagaga
     2581 ctagcgttaa acgcatcctg aataagttca gcaatcggta tgatttcgaa cattaaaaaa
     2641 gcgtccactt gatccgataa cccactcatg tctcccttta gtaggttttt cggaacgtga
     2701 aacgctgctg ctgtcatctc aaagatgtcg tctattaagt tttttatatc tcttgaattg
     2761 ctttggaagt ttccgctgaa atcttctaat gtgtactcat tttgtaattg aaataccgca
     2821 cctgcattat cagcttccat aaaagcctta aattgtgatg tcatcatttt attgatttga
     2881 tcttgtgttg tattgtcttg cggtcggaat aaattccctt tcagtacgta tctacgagcg
     2941 ttagagcgct tgtaaacatt catggcacta gaaatgagtt tcccatacgc ttgataatac
     3001 gcatcgacta gttgcctaat ttgttgatct gcgtatttta tatagataac atcactttct
     3061 agaaattctc tatcaaggac tatgttgtta atttgcactt gagaaaacac atcatctttc
     3121 aatgcatatt ctgtgacatc ccaactatcc gcaataaata tttcgctaga attattagac
     3181 ggagaaacga tcaatacttc attgtagaat attaatctcc tgatcagttt ttttctaaat
     3241 tctgttgcat tatttttctt attaggagct acattcagcc tatagtaaag atcattcttt
     3301 ttattttttc catcttcata tgacttgaat tccgctttac tcatcgcatt tgcaatcaaa
     3361 tcaatacaag tttcaatcgc aaattttcga tacacaaaat caacttgcaa tttacaaaag
     3421 tattcttcta aaggaaccgt tgcttttttt gtgaagtatc ctaccgcctt ttgaaaaatc
     3481 cccactttct cacctccttt caagttagaa tactagagga gtaaatccag ttcctgtatt
     3541 ttctactgag ctatttgtga ctgttacagg agcagaatca taaatatcat ctaaaaaatt
     3601 caaaccatga aggaatgaaa aaaagccatc cgtttttcta gtttcaggtt ctattttttc
     3661 atagcgtata tttccattag aaatatgctc ttcatataca ttcatgcaat accaacgcat
     3721 aatcgcatcg tcaccaaaaa ataaacgttg attaataaaa aggtcatcaa ccagatcttt
     3781 taacatacca tgtgtaacag atccgcttcg aacaatttcc acagtaaaac ctgcttcttc
     3841 taaagcgggc ttcaatattt ttgcacggta catatccata gcgatttttt taatataata
     3901 tttattactc atttcaagaa accaacctac aatataatca gcttctatat tttttccatg
     3961 aacgatctgt gattttcctt gatctataga aatatctata acctctcgtt tgatgttttg
     4021 taatcgaagg gctgattcgt ggataaaagt atgttgtgta aaataaacat ctttatcgta
     4081 ttttcctagc aacccaacgc tggcaaaatc tcgtctatca gcaaaatcga ctgttcctat
     4141 cacttcatcc attttttcag gaaattcttt ttctttcgta tgcagaacat catcatatga
     4201 agcaacagca aatcgtgtat cttccatagg tctgttcatt cgtttggtca tgaacgtaag
     4261 tcttaaacca gcattacgtt gcatttgaga gtattcttga aacattttcc gttttaaatc
     4321 tgcattgtaa ttaatagttg gacaagcttt ttcccacatg tcgggatcat caacttcatt
     4381 atcgttatcc aagcgacaaa taaatggaaa caaactagaa aattctgctc catccttgtc
     4441 aattccaagt tctccagaaa gaatcatttt tgattcttct ataatgtcat caagcggacc
     4501 accacgaaca tgaccattag ttgtatcata aaattctcta taatctcgaa ttttaccacc
     4561 accagaagta gccacattta tcattgaata atcttcattt tcgtgaattt catcaaagcg
     4621 gtttgcacct ggtcgcttcc catcttttgt tctagcattt gccgtgttat aacgaagttt
     4681 gctgtttgta gcgatatttt gaataacttc cttcgtagct ttaaatactt ttttatctaa
     4741 atcaggatga tctttaatta ctttaaatac atcatcaaaa ctagtctttg cttggctttc
     4801 attattggca tagatatcaa tatcataatt tttaataccg tgtttagcgg ttagtagaaa
     4861 gaagttgttc caagaagcaa aaccagtttt accattacca cgtcccatta atgaaagata
     4921 tctattgaac actagtgttt tatctttttt ccatcgaaca ccataaataa aacattgtag
     4981 aaatttttcc cacggaatta attcgaatgg aaagtattgt gctggtatat tgattgaatc
     5041 ctctaccatc tgcttatcga agtaaatatc ttctctagta aagactcttt cttctagata
     5101 attttttagc aataattgct ctttgcatac cttgatagtg ccttcttcta tagctttgaa
     5161 ccaattttca atatgcttat aactcaggaa ttgattcatt tgcttcacct accaattcag
     5221 gagtaatggc aagtttatcc aacatcaatc ccatttgttt gttgacagaa acaagcaacg
     5281 ctactgattc attcttttta ccattctcca gtctaatgcc gttctcggat atatcttctt
     5341 ccagtgatat cgccgtttcc cataaactga tataacgatc aacattatct aagaatggct
     5401 caatatttgt tttctgactt tccaattggc ttattaaaga gcggcgtaat ttttctctgt
     5461 agcgattttg agacaattcg tttttaaaca ttttagccct cctttcatga taaagttcga
     5521 aaaaatctct tttcctgaca gccccctccg tttcatcacc cccaaaaaat ttgcgattta
     5581 ttttaagggg gggttatctc accatcggaa tgaaagcttc agcgaagtca atgtaataat
     5641 taatctcttc aatactatat ccaaaaacat tttttattct ttcgacgtta ttatctttat
     5701 tcaacgcttc tcttacttga ttcactttgt atttactaca acagttatct gataacagat
     5761 ccctaatacc tacatagcga acgtatatca aacgtttaat taatccctga gtataagatg
     5821 aatactcttc aatcttttct gtatcatact ctctgccatt gtcattgatg atcatgcact
     5881 taccaccttt cacttgcatc gaagttagcg aagctttcta tcttcttctc ttgtttatct
     5941 aacgctgtaa gatatctgcc atgaacttca ttatgatgtt caacacataa acaaataaga
     6001 ttatctaaat ctaaagctaa gtcaggtcta tccttgactt cctttatatg atgaacgttc
     6061 tctactctat gatacttacc tagtcttcta cactcttggc attcatagtg atctcgtttc
     6121 atcgctttct ctctaagcct gcgccattta ggagactgat agaacttaac caaacgatct
     6181 tctcttatca actgtaataa ccatctatag aattcctcgg tcatgtccca tctcctttcg
     6241 caatcttatt taatacttag ctattctttt gccatacaat ggaataactt cattgctttc
     6301 cttccgttta tatgtatcgc tctttattgg tcttctatac tttcgtacta tctcaccgtt
     6361 accgttttgc acagtgatta cttcatgctt ctgttctaag tattgtggtc tatacatcgt
     6421 cgtgcctcct ttacgcaaaa taaaaagacc actcaaagag tgatctacga attttaaaaa
     6481 atctcatact gaaatcttat ttttaatgtc tttatataag ttcgagcctc gcaaaacata
     6541 aatatcttga taatattctt tgccattata ttctaagaat aaaaaggaaa atttcaattt
     6601 tttacatttt ttatatctct tttctaattt cttgctgttc tttatgttag ctaccattgc
     6661 agtaactaaa ctttcattta caattggtat ttttagacct ttttctaagt atccaatatt
     6721 ctggaattct tgattaataa ggtctgttgg attgagataa gatatttcgt ggtccacata
     6781 tttttcaata agctttttaa ctacagggta tttgtaaata tatttgtacc aatgctttct
     6841 gataaaacgt tggaaaatgt tatatttacc tataatcccg caaaacttta ttgcggtttc
     6901 agatttccct tcatttaata aagttatagt aaacccatat ttaccgacga cagtaatatc
     6961 ttttttggtt attccttcag ttattcttga tacaatttta gaactattag aataccagta
     7021 gtactttgat gtttgttgaa ttgtaatcac tatcaaaata atacctaccc attcgcctaa
     7081 cgtaccatat ccatcaaatg taaacccaag aaatttaact acgcccacca ttttctatca
     7141 cctcagaaat aatattatca aataatctta gataactaaa caggggactt gtaaatttca
     7201 gataattact cattgacttt tcccattcat cctttattgc gtctaccctt tccgccacag
     7261 cgacaaattg atattgtgaa attttataca tagcttataa ttttaattat cagcgagtgg
     7321 tccgctgaaa taaattatag gtggtgaaaa atttgtactt tgttataaga gaagcaacta
     7381 atggccaata ttacttcgtt attaagtcca ataacaatga agtagtagca actagcgaaa
     7441 catacttgac taaatattcc gctgaacaaa ccattaattc tataaaaaac ggaatcacca
     7501 aagactctca agttatcgat atgactaaat aagacgacta acttcatttg ctagggagtt
     7561 catttcagct gctttttcta ccaaagcagt tgagatactt tctagctttt ctgatttaac
     7621 agaattaaat actggcgtct tgatactaat cgatacatct tgacgagaat caggactcgt
     7681 tgctctattt ttttgcacca ctgatctgta cttttctact gaatgatact caattacttg
     7741 ttccaaatgt tttttgtact ctttacatac agcttctttt tggatcaatt ctaaacgttg
     7801 ttgctcaata atatcaatca accgttcccg atccatacct tgatacttta tttgttcgac
     7861 ttccattcat ttcccctcca atacataaat taattagcta attgttgctc attgatctgg
     7921 agagctattt cttcttctat aagctcaacg atcttccttt ttgtcgaatt taattcgaac
     7981 ttatgatcca ttaaagtaag ctttatgttc ttaatctttt caccttcgaa ttctttgata
     8041 atatcttcga ttctttggtt tactaaaatt gttagttgat caattttttc ggaagcgatc
     8101 ttcattttat ataccctcca atacataaat taatagacag caacggatga tagataataa
     8161 gaacaattta gaaggagttg aaattcacat ccttattctt aatatttccg ctgctgccta
     8221 tcgaagctta attaaacgat gagggagatt tcctccctta cattttattt tgtcgatcct
     8281 gtttcctaat ctttcgacac tatcataata caacgttgaa taggtaagtg attggtataa
     8341 aaaaggtata aaatggaaac caaattggta ataaaagggt ataaaaagtg taaaaactgg
     8401 ctacttgaaa gcaaccagtt ctaacgatga agcaaattgg atgataattc tgtttgattc
     8461 tactttaacc gattcttcgc tagtattatt cctttgagca gttacataaa tgggcagacc
     8521 attgatataa cgatcataga atatcttctt gcgcctttca gtcacatcag gcttatgcgg
     8581 atgctgtatc gctgaatagc ctcgaacaaa caatttatgc agatactcaa attcttcctg
     8641 tgcttcttct ttatcgatca gcattctttc tgcttcaaat atatgatcag ctgtagaagg
     8701 tggaaccaag gaataagatg ctgtcacttt tggttctcga ggttgcccta ctctacatct
     8761 agctgataga tatgctgaaa gaaacacagc gacattatgt tttgtgcgtt ccatatcaac
     8821 atctttcgcg cttggtgtct catatttctt tacatcgaaa agtaccatcc tctgattccc
     8881 ccaattatga tataatactt atgtcggaaa tattattcat agtcggagga atccgacttt
     8941 ttttattttg tacgtgaaat gagttctcct gatttatacg cctcagcaaa ttcaaccaaa
     9001 gctactgcct tcattctgtc tttttct
//
LOCUS       DAZKKW010000098         8889 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.100, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000098 DAZKKW010000000
VERSION     DAZKKW010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8889
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.100"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(504..728)
                     /locus_tag="ACG8PO_002360"
     CDS             complement(504..728)
                     /locus_tag="ACG8PO_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0207648.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            919..3696
                     /locus_tag="ACG8PO_002361"
     CDS             919..3696
                     /locus_tag="ACG8PO_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFQ0207649.1"
                     /translation="MNKLLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNT
                     VSHYLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQP
                     KDLFSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLH
                     DLLEDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLD
                     EVHRLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRI
                     PIRIELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGEL
                     KNIVKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLM
                     EKQQPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHEL
                     LVYWTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELE
                     QQVSQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIA
                     AHGYATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILV
                     DMGSLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPE
                     WRLLYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNS
                     DPLFSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQ
                     RIIRNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLI
                     ERLIRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSE
                     DTEFLSIEEEF"
     gene            3777..4196
                     /locus_tag="ACG8PO_002362"
     CDS             3777..4196
                     /locus_tag="ACG8PO_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFQ0207650.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            4208..4678
                     /locus_tag="ACG8PO_002363"
     CDS             4208..4678
                     /locus_tag="ACG8PO_002363"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0207651.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            4695..5474
                     /locus_tag="ACG8PO_002364"
     CDS             4695..5474
                     /locus_tag="ACG8PO_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFQ0207652.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            5461..6282
                     /locus_tag="ACG8PO_002365"
     CDS             5461..6282
                     /locus_tag="ACG8PO_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFQ0207653.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            6299..7348
                     /locus_tag="ACG8PO_002366"
     CDS             6299..7348
                     /locus_tag="ACG8PO_002366"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFQ0207654.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            7361..8365
                     /locus_tag="ACG8PO_002367"
     CDS             7361..8365
                     /locus_tag="ACG8PO_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFQ0207655.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
BASE COUNT         2962 a         1472 c         1835 g         2620 t
ORIGIN      
        1 ctctatttac ttctttttta atttgttctt gcaatctttt ttgctacgta atttttgtaa
       61 aaaagtgggt tgagagaaat ctaattgatt ttcaaagaag gagtatgaaa atgagaaaaa
      121 tatttattcg tttattgtgg gtactatctt agaggaataa ataatagtgg agaaaactat
      181 ggtttggcgc agattctatc aggtttgatg gaacgaagac aacctcccga catcaagtca
      241 aaagctactt gagattttga tctgaaaata aaaaaagaaa acaaggtagg aacatcaatg
      301 tttctacctt gttttcgtaa tcaatgggaa aaaactaaga gtggtgtacg taatcaataa
      361 aaaggatggt gacaacagta cctctttact ctctgaaagc atcataactc gtttttcctc
      421 attatgccaa taaattgttg ttataccaca tataaggatt tgtaatagct aaaaacagac
      481 aggaaagttg aaatctgtga atcttatttt gtatggaagg atttatagaa cgcaaaaatc
      541 aagataaaga tgaataaaaa tcctgtttcg gctatatatt gcgtatctac gccaataaaa
      601 cgagtttgtt ctgtcatagc catataaagc aaaggcaaaa atacaactac agcgagataa
      661 gatccgatga tttctcgaaa agttttccag gaagtatgtt tcctgttttt atatttatcc
      721 gatttcattg aatagtcata gcctccttat tctttcatat gttgaaattc cagtataaaa
      781 actggaagga actgtacgtt ttttagaata aaaattataa gggataagta ggctgctgtc
      841 tataataaga tatatttttc aattctttat taaagatttt attgacaaaa tgaatccgtt
      901 ttctatgatg gaggaggggt gaataagttg ttgaagaagg aaattttatg ttacttagag
      961 aaccagacag cctttttcga tttagaacat atgaacgagg tctttacagc acaaaggtta
     1021 gctgatcatt ttggggtaaa aagaaacaca gtcagtcatt atttgaacca gttaaatgat
     1081 gagcaactac tagtcaaaat caattctcga ccagtgattt atttccacaa agaagctttt
     1141 gaaaaacaat tttttaagtt acgcacgaat ttttattttt ctatcgatga tctcaaagag
     1201 gaacagcctt ttttcgcgca accaaaagac ttattctctt ttatgatcgg atacgacgcc
     1261 agtttgaaag aaagtatcga acaaatcaaa acagctcttt actatccaga tggcggatta
     1321 ccacttttga ttacaggcga aagtggcaca ggtaaaagtt atttagttaa tttggtctat
     1381 cagtattgtc tgcttcatga tctgctagaa gattctgcac cattcatcac ttttaattgt
     1441 gcacaatatg cagataatcc tgaacttttg actagcaatt tgtttggaca tgttaaggga
     1501 gcgtacactg gtgcagaaga aaataaaaaa ggggcttttg aagcagcaga tggtgggatc
     1561 ttgtttttag acgaggtaca tcgcttaaca ccagaaggtc aggaaaaact gttcacctat
     1621 cttgatcaag gtgtgattta tcgtatggga gaacctaatt tgtcacgccg gatcaaaaca
     1681 cgtctctttt ttgctacgac tgaagagatt actagtcatt tcttaacaac tttcattcga
     1741 agaataccta tccgaatcga actacctgca cttaatcagc gtagtcgtaa tgaacggcta
     1801 gaactgattt actctttttt tctcgaagaa caaagaaaaa tggggagccc tttgtcagtc
     1861 agtggaccag ctttgtcact tttaacaagt cagaaattat ccggaaacat aggagagctg
     1921 aaaaatatcg tcaaagtttc agcagcaaaa gcatatgctg aacagcgtga acagcctgaa
     1981 atccatgtca cgatccatca tttgcaaaaa gaactacttg cgcagcctat tactaaaaat
     2041 acagcacagc aaggaatcta tattacgcaa gaccagacct tagagcaatt aatggagaag
     2101 caacagcccg aacagcagcg aatcgttaaa agttttgagc aaatcctaat tgatttcaag
     2161 aaaaataact gtctgctata ttcttgcgaa ggaaaattaa agcaagaagt gattcaatta
     2221 tttgattttt tgctgtttga gaccagtaga caagaaaaac acgagctgct ggtttattgg
     2281 acacaatcta tccgagagac ctttcgacaa atggaaactg cctatcaaat caaattcgat
     2341 ggaaacagtg tctatgcctt gagttactat ctctatcagc gaagcactat caaatggtta
     2401 ccggaagaac atgatattat ccaactaatc aaagaattag aacaacaagt ttctcagtct
     2461 tatccatcta gttatcacta tgtccaacga attctggaat taagtaagat caaattagat
     2521 attgaagaca caagtatgga tcggatttta ctgacgatct atctaaaaaa agcgaaatgg
     2581 tcaaaagaaa aacgactgcc aaaagcgatg attgctgctc acggttatgc aactgctagc
     2641 agtatggcaa atgtagtaaa tcgtttatta caagaggatc tatttgaatc atttgatatg
     2701 ccactagatg tcacaccaca gcagattgct tcagaaatcc ttgattattg cgaaaacagc
     2761 gatgtttcaa atggattgat catattagta gacatgggat ctctgaaaga aatccatcag
     2821 ttttttaaaa aacagttatc cgtaccgctt ttgattttga ataatgtgac gacaccttta
     2881 gcaatcacag taggggaatg cctgcaaaaa aatgtatcat tagaagagat agcagaagag
     2941 actgttcgac agatccagcc agaatggcga ctgctctatc cagaggaaaa caaacccaaa
     3001 gcactcatta ctacttgctt cactggaatc ggtacggcgg tccacttaag tgaactgcta
     3061 gaaaaaagct tacctacggc ttgtcagttg aaaatcatcc catatgaata tcagcaatta
     3121 aaagataaga aaaacagtga cccattattt tccatatacg aagtcgtagg aatgattggc
     3181 accacagatc ctgatatcac caatatcgac tatctttcat tagaagacct catctcgggg
     3241 gaaaagatgt ctgtactagc tgaatggttg gacactacta tgaatgcttc agagaaggaa
     3301 atattcaatc agcgcatcat tcgtaatttt tcattggaaa aagtactgga ttccgtgact
     3361 attctagata cagagaaggt gatgggagaa atcgatcatt ttatgagaga attagaggtg
     3421 cagttgaatc gaagcatcag caatcccaaa aaattagcgt tgtatgtcca tgtgagctgt
     3481 ctcattgaac gattgatccg acaaatgcca atcgaaacct atcaaggatt agagaaatgg
     3541 aagcagtgtc aaaaagaagg gttatctgct attaaatctg cgtttagtgt cattgaagag
     3601 cattatagtg tcatgatccc caattctgag attgcctata tctatgatat tttgagtgaa
     3661 gatacagagt ttctctcgat agaagaagaa ttttgaacat aagcaagttg ttgccactaa
     3721 ttttggcatg caacttgctt ttttagtatg tgtaagtgaa aagaggagat gaaaaagtgg
     3781 atagagaaat tatcttagct tcccatggaa aatttgcttc agggatatta gattcattag
     3841 agctgatcta tgggaaaaat cattctatta ctgtattcga ttgctatact gatgagaatt
     3901 ttgatttagc ggaaacagta aggcagcttt ttatgaggta caaagataaa gaaatcattg
     3961 ttctgacgga tctttttggc ggaagtgtca acaacgaatt cttacaatat atcaatcggg
     4021 atcatgtcta tctcgttgcc ggattgaatt tgccactatt gattgagcta gtatctcggt
     4081 tggacgctga agtagagaca actgttttga tccagcaggt gctaactgat tcaaaaaaaa
     4141 tgatccagtt ttgtaacgag cggctcaatc agaaaataga agaagacgaa ttttaggagg
     4201 aagaaaaatg attgtattga caagagtcga ccatcggtta ttacacggac aagtagcttt
     4261 ttcatggaca caaacaatag gcgcagactg tattttgatt gctaatgatg atgtacctac
     4321 gaatgagata cgaaagacaa cgatcaaatt agccaagcca caaggagtca aactagtgat
     4381 caaatcaatt gatgactcga ttgccgcgtt gaaaagcggg gtgacagata aatataagtt
     4441 attcatcgta gtggaatcga tcgaggatgc atataagctg gcagaagcgt atccagaaat
     4501 caaaaccatc aacttaggag ggatcaaggc aaaacctggt actcgcagca tcggaaaagc
     4561 ggtaaatatt ttaccagaag aggaggaacg cttgaaacga ttaattgaca aacaaataga
     4621 agtagaaatc cgtcaagtac cagcagataa aaaagtggat gtaagagaag ttttgtaaga
     4681 aaggaagaac ctcaatgatt gtacaagcag tcttattagg aattgttgca tttgtcgcac
     4741 aatcagaata tgcactaggg acatcgttgc tttctcgtcc gattgtgacg gggctgttta
     4801 caggaatcgt gttaggtgat gtaaaaactg ggatcattat gggcgcaact ttagagttag
     4861 catttatcgg atcgttctca gtaggtgctt cgattccacc tgatgttgtg accggtggga
     4921 ttttagggac ggcttttgct attacagctg gagccggtac agaaaccgcg cttttactag
     4981 gtttgccgat tgctacgttg acattgatat tgaaaaatat ttacttaggg cttttgattc
     5041 caattatgaa ccataaagca gacaattatg cagaagaagg aaattataaa ggagtcgagc
     5101 atatgcactt gcttgccggt atcggtcttt ccttgatgct tggtgttgtg gtaatggttt
     5161 cttatatggt gggaagcaat acgatcggaa atctattaaa tatgattcct gacttcgttc
     5221 aaaaggggtt gtcagtagct acagggttga ttccagctct tggttttgcg atgcttgctc
     5281 gtttgctgat caacaaacaa gttgcaccat attttttctt aggttttgct atagcttctt
     5341 acttagagat tcctgtgacc ggagtagcta tttttggtgc gattttagca gttgtggtag
     5401 tcaatattat gaatgtccgt caactaccac ttcaaacaac tgcaggggag gtagaagaag
     5461 atgaagaatt ctgagatcaa aaacagcaaa ataacgaaaa aagagctaaa tcacgtattt
     5521 tggcgttctt ttcaaatgga gttttcttgg aattatgaac gtcagatgaa tatggcttat
     5581 gtttatgcaa tgattccgat tttgaaaaag ttatatcagt cgaaagaaga aatggcagca
     5641 gctttgaaac ggcatctaga gttttttaac acgaccccac atatcgttac actgatttta
     5701 ggaatcacgg ctgcaatgga ggaagaaaat aaggatgatc ctgaatttga tgtgactgcg
     5761 atcgatagta tcaaaacatc tttgatggga cctcttgcag ggttaggaga ttctttcttt
     5821 tggggaactt tgcgattgat tgccacaggt gtgggtacct ctttagccat gcaaggaaat
     5881 attttaggcc ctatcttgtt cattttgatt ttcaatattc cgcacattct gtttcgctat
     5941 ctagcaacag gttggggata tcgcttagga acgggttttt tgaaaaagat ccaggcaaac
     6001 ggtatgatgg aaagtttgac attaggcgcg tctattattg gactgatggt cgttggagca
     6061 atgactgcca cgatgatcac catcaatatt cctttaaaaa tcggttcagg agaaaatgct
     6121 gtgactgttc aaagtatttt tgatgatatc gttccaaata tcttatgtct tggagcattt
     6181 ggcatagtgt tttatctatt gaagaaagaa gtgaaaccat taacgatctt gatcggacta
     6241 gctttctttg gaatttttgg ttcgtggata gggatttttt aaaaaaggag cagagaaaat
     6301 gtttacgatg caagattata tctatgaaga aaaggaagta ctaagtacga tcttgaaaaa
     6361 gaatgatttt tcgacaagag aacatatgaa aaaaacagtg aatttactga tcttagcaac
     6421 gggttcatct tataacgctt gtttagcagc gaagccagca ttggaatctt atggtgatct
     6481 tactgtcgat attcaagaac ctttttattt tttcaattat ggaaaacttt ctccttcaat
     6541 cgatactgtg attgctgtgt cacaaagcgg gaaaagtgct tctactgttg aagcagtaaa
     6601 aatgatacaa aaacaagggt tacctgttgt tgctatcaca aatgacgtgc aaagtccact
     6661 tgctttagaa gctgcacaaa ttattgattt aggtgccggg gtagaatcag taggcttcgt
     6721 tacaaaaggc tattctgcga ctgttttaca gttactactc ttagggatag gaattggaat
     6781 aagtaaaaat aaaatcagca aaaaaataga acaagactac atgcaacagt tgcgcaaaat
     6841 aatcaaccat ttaccagcga ttattcaaaa aacggaagaa ttttttgatg gatatcaaag
     6901 tttatttcgt ttagcacagc gtttcgtcac cattggatat ggtccaaact ggggaacagc
     6961 taaagaagca gagacgaaat tgactgaaac aatccgcgtt ccgtcacaag gatttgagtt
     7021 agaagcttat atgcacggcc catacctgga ggcagatgct tcgcatttgc tattctttat
     7081 tgaaggggaa agtgtaaata aggaacgttc gcaaaagttg caaagatata tgagtcggta
     7141 cgttggagag actctcacca ttaccacaaa aaaagcaaga aacgagaaaa cacttggatt
     7201 agcaatagaa tgtgatgaat atctgtctgt attagcgctt gtcgttccgt ttcaactatt
     7261 cgcttataaa accgcagtag ctaaaggtat tgacttgaac aaaaagattt ttgaagactt
     7321 tgatacagtc ttgaaaagta aattataaga ggtggaaatc atgctgaaat tcaatgaaga
     7381 agaacaaatc aaagcaataa aaggcgcact agctttacgt ccgcaagtag aagaaatcat
     7441 cgataaactt tatgcggaaa aatttgacgc tgtctattat ctaggaatcg gtggaacgta
     7501 tgcttcctcg atgcaagctg tcacttatat gaatgggaaa agcaacttgc ctgtctttgt
     7561 acagcatgct gcagaatatt acactactgg aaacaaaaga ttaacaaaag attctatcgt
     7621 tattttgtcc tctgttaccg gtacaactca agaagtagtg aaagcagtag aacaaatcaa
     7681 gaaagtagga gcgacactga ttggatttat cgataaagcg aacagtaagt tgtctcagct
     7741 atgcgatttt gtggtcactt atccagctcc tggaacagaa caaatcaaat tttttatggc
     7801 agcagatcga ttgatgtatt tgaatggaga attcgaagct tattcagaat actatgaaca
     7861 gctcgaacgt tatttaccaa cagggttagt ggaagcagag aaaaaagcag atgcatttgg
     7921 cttatcattt gcagaaaaac atcgacgtga tagtatgcat tattttattg gagcagggaa
     7981 tcaatgggga gctgtttatt catatgccat gtgttactgg gaagaacaaa gctggcttcc
     8041 atcgaaatct atccatgctg ccgagtttct tcacgggacg ttagagatcg tagaagaaac
     8101 aaccccagtc acattatttt taggcgaaga tgaacagaga gtgttatctg aacgtgtggc
     8161 taaacttttg ccaaaaattt gcagcaatta tacattgata gacacaaaag actatccagt
     8221 agaaggtatc agtgaaaaat atcgcggacg tgttttgtct ttcttattga tgcatgtagt
     8281 gacacaacgc atcgatgctc atgtggagca gttgaactgc catccattag agatacgccg
     8341 ctactatcgt cagtttgatt attaggatag actggataaa aagaatataa aaaataaaga
     8401 gagcagcgat aactgctccc tttatttttt taaaatattg gcatcaatta cgttataaaa
     8461 cacatttttt gcattatcta cagttcaact tataatctgt ttaatgggac atgtgggtgc
     8521 caaccatttt ttgttatgca atagtactat ttagttgttg catggatccg tagtaaaagt
     8581 ccaagtacaa cgagttgcgc cagtttacat agcgttttgt atccagaatg ttgactaaaa
     8641 caaaagttcc tatgtcaacc gtcaacagag acaatctgtc cgttgcagga cctgacagat
     8701 ttagaaatgt tcaatctgtc tcttatgtat tttaaatcaa tactcactag gagaagtttc
     8761 ctaacctcct aatagtaatg ctggtagtaa cattgtcaaa actgcttcct cttaggaaca
     8821 agcaaagcat aacgagtgta aaaataaaat gtagactatt gtaacatatc attgattatt
     8881 tcaagtaaa
//
LOCUS       DAZKKW010000099         8439 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.101, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000099 DAZKKW010000000
VERSION     DAZKKW010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8439
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.101"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            363..1802
                     /locus_tag="ACG8PO_002368"
     CDS             363..1802
                     /locus_tag="ACG8PO_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0207656.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
     gene            2097..2315
                     /locus_tag="ACG8PO_002369"
                     /pseudo
     CDS             2097..2315
                     /locus_tag="ACG8PO_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
     gene            2564..2779
                     /locus_tag="ACG8PO_002370"
     CDS             2564..2779
                     /locus_tag="ACG8PO_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0207657.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(2996..3252)
                     /locus_tag="ACG8PO_002371"
                     /pseudo
     CDS             complement(2996..3252)
                     /locus_tag="ACG8PO_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(3439..4350)
                     /locus_tag="ACG8PO_002372"
     CDS             complement(3439..4350)
                     /locus_tag="ACG8PO_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFQ0207658.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGNWKIVDVK"
     gene            complement(4369..5373)
                     /locus_tag="ACG8PO_002373"
     CDS             complement(4369..5373)
                     /locus_tag="ACG8PO_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HFQ0207659.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            complement(5370..7493)
                     /locus_tag="ACG8PO_002374"
     CDS             complement(5370..7493)
                     /locus_tag="ACG8PO_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFQ0207660.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            complement(7498..>8439)
                     /locus_tag="ACG8PO_002375"
     CDS             complement(7498..>8439)
                     /locus_tag="ACG8PO_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFQ0207661.1"
                     /translation="RGGWKETLPEIADEINILNLTSKPKNQGLLDPYVIMEKKKDSES
                     LAIDILTFLTGISSRDGEKFPVLRKAIRTVTQSNQRGLLQVINELRKEGSPVAENIAD
                     HIESFTDYDFAHLLFSDGSITQSISLDRQLNIIQVADLVLPDAETTFEEYTTMELLSV
                     SMLIVISTFALDFIHSNREIFKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYF
                     VTQNSDDLLDERLKNNIGLKFAFRSTDIHEIKKTLEFFGLDREDESNQKRLRDLENGQ
                     CLMQDLYGRVGVVQVHPVFEELFHAFDTRPPVQERME"
BASE COUNT         2423 a         1720 c         1559 g         2737 t
ORIGIN      
        1 gaatatcata gcaatgtatt tgaaaagcaa attggaaaac aaataggtgc aacaccataa
       61 atatgaaaaa attgggaaaa agtagaatgg aaatttgctt acgtctcacg agatagctca
      121 cactattaat ctgaagaaca atgaagtcga cttttagaaa ttgctagaag aaagtaaagc
      181 gaattatcac tagttactat agagtaagtt caaataaagc atctccaact tacatggtta
      241 catgaggata tcaaatcgaa attactcaag cttcctatgg ctagagtcgc gagaagttgt
      301 tgggcagtag acataggtta tattgttctt agctacacga tcattagaaa ggaaatccta
      361 ttatgtatag ttatcaagaa gatgcaaaag tccaaaaaaa tcgctggtgg attttgattg
      421 ctatatctat gtttaccttc atgtccacat tggatggcag tattgtcaat attgcactac
      481 ctaccatctc agatgatatg ggcataccaa tgaatcaagc ggaatgggta gtgtccgttt
      541 atctgatgat tgtctgttct tgtttgttgc tgtttggtaa aattggcgat agccgtggga
      601 aaatcaaagt atttcggatt ggtacgctga tctttgtctt gggatcgctg ttgtgtgggt
      661 tcaatcattc gctaggtatt ttattgttcg cacgggtggt acaagccatt ggtgctagta
      721 tgactatggc tacaaatgcc ggaatcatca cggaagtctt tcccatacat gaacgtggtc
      781 gggcattggg agcaataggt gcgtttgttt cattaggctc gattgctgga ccaggtcttg
      841 gcggcttgat tttgtcccaa ttttcttggg cgtacatttt ttggatcaat gtaccggtgg
      901 gaatcgtaac gatgttgatt gctgaaaaat tcttaccaaa agatatcacc atgagcggcc
      961 atacaatcga tggagggggc tttgctgcca ttgcggctac gattatcagc ttttttggag
     1021 caatcttttt ggggcaagaa agtggctttt tccatccagt cccccttgta ctgttcgtaa
     1081 tttcagtgat tgcctttgtc agctttttaa aagttgaaaa gaaagcagcc tctccattga
     1141 tttctttcgg tatttttaag aacaagattt ttaccatgag cttgattaca gccttgttga
     1201 ttttttcttc caacttcttt gtcaatgtag tgattccttt ttatctgcaa aatgctcgcg
     1261 ggttgagtcc tagctatgct gggctgttga tgatggtctt tcctttgatg atggtcgtcg
     1321 gggcgccttt aagcggctac ctgaccgacc gggttggccc ggagattttg gtattagtag
     1381 gcttgggctt attgtcggta acccaggtca tgtatatgtt tttgtcagtg gactctcctt
     1441 tgtggctata cattgtcgct actgccatca tgggtgccgg caatgccttg ttccagtccc
     1501 ccaataatac gatggttatg agctcggtca ctatcgaaaa cttgggactg gcaggctcga
     1561 tgaattcctt tgcccgaaat ttggggatgg tcagcgggat ttcgctttct acgacggtct
     1621 tatatcaagc gatgtctgct aagatgggcc agcaagtaac taactatatc gcaaatcggc
     1681 cggatgtctt tttatatgga atgaagatta cctttttagg cagctttgtt ttgtgttttg
     1741 ttgccttttt cctgactatc tggcgtatca aaaaacaagg aggtattcag aaaaatgtct
     1801 gaaaaaacca ctgttgaaga atttttggag gcctgcgtat gggtgagtgg gatttccatg
     1861 gatgatggaa gatgaagtga acttctaaaa gccgaaaaac tcaagtatta tcttcaaaat
     1921 actagtgctc aatgagatgc ttgatttgat cacaagaaga ctgcgaaagc cgctggcaac
     1981 cgcaagctag gtatcccacc gatggactac gttttagagg ctactggtta tgaatacgga
     2041 gccaacgctc tcatctgcat aagaatacat cacccagatt atgtaaagat tttcgatact
     2101 tctgtcaaaa aatcattaag atagataaat gatttccgtc tagtcaaatt ggttcagaat
     2161 gtagacacac agatggtaag aagtcactca caattcgaga atggacttgt cctatttgcc
     2221 gtatacatca taatcgaaac gtcaacgata gtatcaatat tttgaccaag ggctatgaat
     2281 acaagcattg ccttagaaaa catcaagaac cttaggaaca acggggatag cttggttaat
     2341 aagagatacc gctgtttagg cttgcaagta tactctttta tcaagaattc cgtggcttta
     2401 gtcatgagag gcttaatact atgaaaattt tctttagcat ttttgattta ggttcctttt
     2461 ctaattataa taagatgacc actacagtaa cctactgaaa tatataaaca gccactgtag
     2521 taccattttc aaagtagtct atgagaggag aatcttcatt cttatgaacg ctggaattgc
     2581 ggatatgaat ttaataaaaa aaacccttaa tgattttact tccaattcaa tatcaaaagg
     2641 tactggtatc aatttaagca caataaaaaa gctgaaaagt ggtgaaagat cagtggaaaa
     2701 gctgaatctt ttagatgcta taaaaataac ggaatttgct atgaaaaatg gaaaagctga
     2761 gattgaaata tggagataat tatcgtctag tgaattactt gccacttagc tgaatttaac
     2821 gatccttggt gcttgaaggg ggagattctt gggaatagag catacttgca agcctatgtc
     2881 tttactaaca acgatatctc ttattaacca agctatcccc gtagttctaa ggcttttact
     2941 tttatctaag caataatcgt attcaaagga aatttttgac ccttagaaag tctcatttta
     3001 tcagacgtga gcttaggcat tgctgaattt tgttaattcg gttttaggtt gtgctttgag
     3061 cataatatga aagtattctt tatcacgatt tcattcatct aaggtaatgt gatgtgattc
     3121 tgaaattctt tcaaaggcag tttttgcata agctgacatt acatcatcaa tgacatgttt
     3181 acaatgtttc actactaaaa taagggggta accaagaaga aaaactgaat gattatttgt
     3241 atctaattcc atggtatatc aaccatttcc tatatagact gattatgaca tggattaaaa
     3301 taaaaaggca ctatgtctta attgtcggtt ttcgagttac cgaatggcat tcatcaccca
     3361 cctatagagg atgggcgact tctgcctgaa ataagttaca ctacttataa cttctaataa
     3421 aattagattg agtcattatt atttaacatc aacgattttc cagtttccat ttttttgtaa
     3481 tactaatttg tactgaaagt agctagtggt ttttgataca ttatcaaggt attttatctt
     3541 tgtactcact agaaaatttt ttccgttttg tagataaact ggattctcta actccgaaaa
     3601 ctttaagtca atattgattg gtcgcatcgc accattttca acataatact ccaattcttt
     3661 ttcagaagct gatggataaa gtttaaaaaa tgtttctaaa aaatcggtga tttcttccgt
     3721 tgttttggca tcaatttctg aatcgttttc tacttgtttt gcttcatatt ttgccttgtc
     3781 tggctttcca atcattgttg gcaaactagt tactaaatga ttcctgtttt catcttcaaa
     3841 gattgtcact cgatatgcag aagaaattga ttgcgtcttt tttccctctg taatatgttg
     3901 ctccactaaa aatgaaacga caaattcttc cgagtgtttt tcaacatcta gaatttttac
     3961 agattgaact tcggaactcg tgggaatatc tgctcgaacc atatcttgga ttaaagcgag
     4021 accgttttct gtaaggtact gttcgagatt actcattctc ttttcaattg cttctttgtt
     4081 atttttccat gaaaaatatt ctttcgcaaa atcctctgta aaactttcaa cgccagaagt
     4141 attcactacc ttctctttta taaccacttt ttcatgagtt gtatgttgat caatggcggt
     4201 gaaattttta taaacgccaa aagcaagact acaagacaat aaaagccaaa gggcaagcac
     4261 catttttcga tgcttaccca cggatacaac tcgttctttt ttcttttttg ggctcttttc
     4321 cttttgattc cgttcaattt ttatcttcat tctttttcct cttttctctt atttgtatcg
     4381 gccagctccg ataagatgct gccgccaata gttttccgtt agattcgtcc accccactgg
     4441 atctcctgca tgaaacatgc gattctcccc tgcgtaaatg cccacatggg tcacataagt
     4501 accggcatca taggttgaat gaaagaaaac taaatcacct ggtttggcat cttttagatc
     4561 aatatgagtc atggcatcat attgatcctg agccacccga ggaagtttca ggtcaacttt
     4621 ggcataacac cattgggtca agccactaca atcaaacccc gtttctggac ttgagccacc
     4681 aaacacatag gccgttcctt catacttaaa tgcctcatca aaaatttctt ggactatttt
     4741 gtcatcaaac tgagttgtag ttaggtattg cttcactaat aaacagtaaa acatattgcc
     4801 ataattatac cgccacccac cattttcctt aatcgcgatt gggttggggt aatcgacttt
     4861 ttgaccacct gatttttcct ttgaaaaaga ttcagctaaa gcaaaactat gcttcttccc
     4921 gtttttcccg acatgatcta gatacccgcc accatagtta taggcctgaa taatactatc
     4981 ttgatcacac cctagttctt tggaacgctt aaccaaagac gcaaaataga gacagccttg
     5041 tttgatggat gcctcggtac ttaatgaatt aggtggtaat ccggctgact cagaactttg
     5101 cataacgtct tccattgtgc caccagattc cacttgaata atcgccaaaa gaattggaat
     5161 atactctgtt atatccgatt ctttggcata cttttccacc attgcacggt gtttcaatac
     5221 ttcttctgag acactagttc cgccaggaga agtgccagaa ctaccatttc cctcactgtc
     5281 ttcaccaaac aacaaaccca cacacatcaa aatacccata aaagagatgg tcagaaaagt
     5341 taatgaaata acacccactt tcttcaagtt cattttttct cacctgacct tgattttgga
     5401 cgatggttca ttcttttttg tactttttga ttggtctgct ttggcagagt ggacctctgt
     5461 ttcacttgtt ggaaagaatt tttcttactt gctgattggc ttttcccttt tgaaatgatt
     5521 tcttgactag ttagttttcg tttgatttcg ggattcagat ttgctctcac aatccgcttt
     5581 ggtgcttctt ttttcagcat catatctttc tgaggttgag ctgctttatc tattggaata
     5641 ttcgcccttc tttttccctg acgatttgtt gcttcactca gtatcttgcg tttttcgtcc
     5701 attttcgcat ttcttcgttg tcgttgtttt tctcgcaaat ccactcgatc cgctttttct
     5761 tggaacagtc cgcgcttgaa ttcttctggt gcttttttcg acttttcgat tcctttatgg
     5821 agattgtatt tcaaattcgt tggcgtattc cttacctgat cctttacttg tttagtctta
     5881 tctttcacca aagcttgggt atccaaaact ttgccgattt ttctcccagc caaattcatt
     5941 cgggattgtt tcttattatt tgaaactgga ttctccttag ttaagtcaga tagtatttcg
     6001 tgatcgttcg gtaatcgtga attctttcgt agagaagaac cggatggctt aaatttggat
     6061 tttgattgtt cctttgccaa cttatttccg actgctgcac caccaccaaa agccaaagct
     6121 cgtcccacat ttcgattgag ttgccgcaat tttcgattca ttaacatttg cggttttcgc
     6181 atgacgcgtc ttcccattga ctggctatca ttactctgga ggttgaacat actcataatg
     6241 tcgcccaatt tgaaatagat accggcaaag gtcacaattt gcagaaaagc aaccatgaaa
     6301 aatggatagg ttgccgaaat attgaaaaac atcgtggaga tgctaaatgc agtcgtaatc
     6361 accaaagtaa cacccgctcg catcataatc gtattgaata aacgaataat cgccttcttt
     6421 cctaaacttt cataagtcgg caacatggac aataagaaac taattggcag aaacatcgca
     6481 aagataatga agagaatttg cgagaacaac ataatccctg ttagaagaaa aacaaagaag
     6541 gaaatgccaa tattgaacaa taccaaaaag accacggtcc ctaaacggtt catggtcttg
     6601 gtaatactga gattcgcatt gtcattgtct tcaatctctg ccttgaccgc ttcttcccga
     6661 tctttgcctt tgttttcatc tggactcaca gatagaatct tgttgacccg gtcttcacca
     6721 atttcttcta tgttggaatc atcaaattgc agtagtaacc aaggttgttg gacttgaatc
     6781 gaaaacaaac tatcccgaat caaatccaca ctatcttttc cttggctttc tgaattgggt
     6841 acaacgatct cggttccaag ggtaagggcc gcttcactaa catctgagct aaagtcattg
     6901 atttttgtga tataggtagg cgcataagca atgaacgaac ccgatagtag aaaaatcatc
     6961 acaaaattaa tcacggctcg aactgctttc gtggtttctc gttttaataa tccggtatac
     7021 gccacataca cccccagagc taaaatcatc aataatagaa acccaaaata aaatccggag
     7081 gtctggagtc cattttcagt aataccagcc aatgtttgga tattctttcc aatactttca
     7141 gccgtatccg aaatgaaatc taatttgtaa gcctcttgaa ccacataacc cgtggcgttc
     7201 gacaaataca aactcaccgt ccaaatgaaa ttagtaatgg cataaagacc atacatcacg
     7261 cttttcccta agccatcacc ccaattccaa ggcaaccaat cccaagagct atccacaaag
     7321 aaatctagtt gatagttgtt cagtgaatat ttggaatata gatttcctgc ttcaactgta
     7381 tcatcaacta gccctgtcgc ctcggctacc gtaccaacta aactcaatag aactatcaga
     7441 gtgagtatgc taataaaaac gattcccaca atccgaaaaa gtttcttttt catcccctca
     7501 ctccattctc tcttgaacag gtggacgggt atcaaaggca tggaagagtt cttcaaagac
     7561 tggatgtacc tgaaccacgc ctacccgacc atacaaatcc tgcatcaagc attggccatt
     7621 ttccaaatct cgtaggcgtt tttgattgct ttcatcttct cgatccaagc caaagaattc
     7681 caaggttttc ttgatttcat gaatatcggt actgcgaaac gcaaacttca acccgatatt
     7741 attcttcaag cgttcatcca gtaaatcatc actattttgc gtgacaaagt aaaccccggc
     7801 attcatagcc cgcccagcac gaacgagctt attcgataat gttttccctt gcgctacttg
     7861 aagaaaactc cacgcctcat ctaagtctac aattttgaag atttctcgat tactatggat
     7921 aaagtccaag gcaaaagtgg aaatgacgat tagcatggaa acactaagca attccatggt
     7981 ggtatattct tcaaaagtgg tttctgcatc tggtaaaacc aaatccgcta cttgaatgat
     8041 atttaactgc ctatctaaac taatcgactg ggtaatcgaa ccatctgaga aaagcaaatg
     8101 ggcaaaatca taatcggtaa aggactcgat gtgatccgcg atattttccg caactggtga
     8161 gccttctttt cgaagctcat taattacttg taataatccc cgttgattgc tttgggtaac
     8221 cgtccgaatg gccttacgca acactggaaa cttctcccca tctcgactag aaattcctgt
     8281 taaaaaggtc agaatatcaa ttgctagact ttcagaatcc ttctttttct ccataatcac
     8341 ataaggatca agtaatcctt gattttttgg cttactagtt aagttgagaa tattgatttc
     8401 atccgcaatt tctggcaagg tttctttcca gccaccccg
//
LOCUS       DAZKKW010000100         8229 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.102, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000100 DAZKKW010000000
VERSION     DAZKKW010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8229
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.102"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            329..1252
                     /gene="truB"
                     /locus_tag="ACG8PO_002376"
     CDS             329..1252
                     /gene="truB"
                     /locus_tag="ACG8PO_002376"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFQ0207662.1"
                     /translation="MDGLLTLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEEKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVKRFKRVDITDEVWQKVKNGMRLNYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            1256..2197
                     /gene="ribF"
                     /locus_tag="ACG8PO_002377"
     CDS             1256..2197
                     /gene="ribF"
                     /locus_tag="ACG8PO_002377"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006747 - FAD biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HFQ0207663.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGEIWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            2592..3377
                     /locus_tag="ACG8PO_002378"
     CDS             2592..3377
                     /locus_tag="ACG8PO_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFQ0207664.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWINNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENDKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            3390..4241
                     /locus_tag="ACG8PO_002379"
     CDS             3390..4241
                     /locus_tag="ACG8PO_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HFQ0207665.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(4365..4817)
                     /locus_tag="ACG8PO_002380"
     CDS             complement(4365..4817)
                     /locus_tag="ACG8PO_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0207666.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQIREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNHVVNFSQWINEKLLMQTDISLVS"
     gene            complement(5282..5650)
                     /locus_tag="ACG8PO_002381"
     CDS             complement(5282..5650)
                     /locus_tag="ACG8PO_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HFQ0207667.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            5800..6282
                     /locus_tag="ACG8PO_002382"
     CDS             5800..6282
                     /locus_tag="ACG8PO_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HFQ0207668.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            6319..7329
                     /locus_tag="ACG8PO_002383"
     CDS             6319..7329
                     /locus_tag="ACG8PO_002383"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S66 peptidase family protein"
                     /protein_id="HFQ0207669.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPPWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQPSEKYQKEWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTISHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(7444..8157)
                     /locus_tag="ACG8PO_002384"
     CDS             complement(7444..8157)
                     /locus_tag="ACG8PO_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0207670.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIKQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLINVIVMDN"
BASE COUNT         2785 a         1337 c         1667 g         2440 t
ORIGIN      
        1 attaagaaac tgaaaaaata aataaaaaaa aacggaaaaa agactggaag aatggacagt
       61 cttttttttc gtttttggga agtgaaaacc aaactattta cctgtaagcc acccataaat
      121 ccaatcaaat ggtgcaaaga aaaaatcttc cattctattc cctcctttaa aaaaaacata
      181 acatgatttc tatttacgtg agcatgaatt ttgttacgtt tttattgccg aaaaataaaa
      241 accgttcatt atgatgtcaa attcgtcagt aattgatgca atgtgttata ataattcagt
      301 taaatcaaaa atgaaatgga gaaaagacat ggacgggtta ttaactttat ggaaagaacg
      361 cggaatgaca agtcatgatt gcgtattcaa attaagaaaa atcttacata cgaaaaaaat
      421 cggacatggg ggtacgcttg acccagacgt agacggtgtg ttgcccattt gtattggaaa
      481 agccacaaaa gttatcgaat atttgacaga ttctgggaaa atctatgtag gcgagataac
      541 tctaggtttc agtacaacga cggaagatcg atccggagaa cgggtagaaa caaaaccagt
      601 agatatgtct ttatctgaag aaaaaataga tgaagtgatg gcttcattta ttggtgagat
      661 cacccagata ccaccaatgt actcagcggt aaaagtcaac ggtaagcggt tatatgaata
      721 tgcccgcaat aatgaaacag tggagcgtcc tgttagaaag gctcagattt accgttttat
      781 ccggacaagt gaattgacaa gagacgaagt gacaggaacc atttcttggc gcttcgaagt
      841 agaatgcggg aaaggaacct atgtccgcac attagctgta gatacaggca agaaacttgg
      901 ttaccctgcg cacatgtctg atctgacccg aacagcaagt gccggtatgt cccagaaaga
      961 tgctctgaca ttagaagaag tagctcaagc tgtgaaaaat gggacaatcg atgattgttt
     1021 gctgccaata gaaaaaggag tgaaacgatt caaacgagta gatattactg atgaagtatg
     1081 gcagaaagta aaaaacggta tgagacttaa ttatactgta tttgcgctcc cagaaatgcc
     1141 gtcagaagaa attgctttgt tttataaagg caaagttgtc agtatctatc agccaaaccc
     1201 gaaagaaaaa aacaaattaa aacctagtaa agtattgaga aatgaggaat aacgaatgaa
     1261 tatcatcaag atcagacatc cttaccaatc agaacaaatt ccagaagacg atgtcgtttt
     1321 agtactagga ttttttgacg gcgtgcatat tggacaccag aaagtcataa agacaggtat
     1381 ggaaattgca caaaaagaag gattgaaatt agcattgatg actttcaatc agcatccatc
     1441 gattgtcttt aaaaagatcg atcctagcag cgtgaaatat ttgactagcc ttaagcaaaa
     1501 agaagaaaaa atggccgctt tgggtataga ttatttatat gaagttgatt ttacttcttc
     1561 ttttgctcat ttagcaccgc aggaatttgt tgatcaatac atcgtagggt tacatgcaaa
     1621 atatgctgtt tccggttttg attatacgta tggaccaaaa gacattgcag atgtcgctca
     1681 ttttcctgaa tatgccaaag gaagattcaa aatcgtcaca gtttctaaag aagaagaaca
     1741 aggtgaaaaa atcagctcta cccgtattcg aggattacta gacagcggac aagtaaaaga
     1801 agcagcaaaa ctattaggtt gcacatatga ggtagaaggg gtagttgtcc atggcgatgc
     1861 acgaggcaga ctactaggat tccctacagc aaatgtcaaa gtaaagagca cagttcattt
     1921 gcctaaagaa ggtgtttatg tatcggaaat aaaaattggg gaaatttggt atccagccat
     1981 gggttcaatt ggacataatg atacattcgg tgaaggacgg gagctgactg tagaattgta
     2041 tattttggac tttaatcaag atatctatgg tgaacatgtc gcagtacgct ggaatcaatt
     2101 tttaagagat caagtgaaat tttctagtgt agaggaattg attgaacaac tcaaagagga
     2161 tgaacaagca acagctgaat atttttctca gcaataaaac attatgcata aaatctcttg
     2221 tttatgtata actagcaatt tcaataaaat gggtgataaa taagcccagg aaaggggctg
     2281 tgacaaaagt cttgtcacag cctttttttc caaataaatg gtttttcaga agtagcttct
     2341 tcggaaataa gccgaaattt cacaaaaatt tgagaagtaa tttttgtaaa ttacttctta
     2401 tttcttgaag ctggccactt ctgtcacaac ctctttcttt atagaaataa aaggacacta
     2461 gcaattcatg aaaacgattg ctaaagcgta tcatttgctt tagactatat atcttggtgt
     2521 acactcactt taagacggaa ataaaagtta tccgtaataa caggtttagg tatgaaaaag
     2581 gagtgggtac tatgtataag gaattagaaa acaaagtcgc agtagtaact ggtggctcaa
     2641 aggggattgg aacagcgatt gctcaacgat tcgggaaaga aaaaatgaat gtagttgtca
     2701 actataactc ggataaagaa ggagcagaaa aagcagtcaa agcaatcgaa gaggcaggcg
     2761 gaaaagcagt ctctgtacaa gcagatgtca gttctgaaga aggaatacag cgtttacttg
     2821 atacggcttt agaaaatttt gggacactgg acgtttggat taataatgct gggatggaaa
     2881 atcagcatcc aacccatgaa cttagccttg aagattggga aagagtatta aaagtcaatt
     2941 tgactggcgt ttttatcgga acgaaagcgg ctttaaatta ctttacagag aatgataaaa
     3001 aaggaaatat catcaatatg tcttctgttc atgaacaaat cccatggcca acgtttgctc
     3061 attacgctgc ttcaaaaggc ggagtaaaac tattcaccga aacggtagca atggagtacg
     3121 caccaaaggg aatcagaatc aatagtatag gacctggtgc tatccgtaca cctattaatg
     3181 cggcaaaatt tgatgatcct gaacaaaaag aactcttgga aagtatgatc ccactaggac
     3241 gcgtaggtga accaaaagaa atagcggcga cagctgcatg gttggcttca gacgaatcca
     3301 gttatgttac aggtattaca ctatttgcag atggtgggat gactttatat ccttctttcc
     3361 aaggtggaaa aggataaggg tgaataaaaa tgggtttatt gattgcattg atcccggcag
     3421 ttgggtgggg gatccagcca ctgattttga aaaagattgg tggcagacca acaaatgaga
     3481 tattaggaac tggtattgga gccgtgctcg ttggcttact tgttcaatta ttcgcttctc
     3541 cagctggtat ttcagtgact acatttttga ttagtttact ttctggtgca ttctgggtca
     3601 tcggacaaac aggacagtat acatctttca acttgattgg tgtttctaaa acgatgccaa
     3661 tttcgactgc attacagtta gtcggaacct cattgattgg tgtcttcgct tttggagaat
     3721 ggtctggaag tatgggaaaa atcattggtg cagcagcaat cattcttctt gtatttggtt
     3781 ctgctttaac ggcaatcagt gatgatcctg gaaaaaaagg cggtatcaca aaaggaatca
     3841 gtctattagc atcaacaagt attggttatt gggtttacag tgcattgccc aaactagttg
     3901 atgcaagcgg actttccatt ttctttccgc aaatgatcgg tgtcttttta ggggcagtga
     3961 tttatgtgat tttccgaaaa ccaaccgcat tttctgaaga caagagttgg aaagcaacaa
     4021 tagttggagc catcttcagt gtgtcagcat tggcttatat tttttcagca aatgaaaatg
     4081 gggttgctac tgcatatatc attacacagc taaacgtagt tatctctaca ttaggcggta
     4141 tgtacatcct gcatgaaaat aagacaccaa aagaaaaaaa attaacgatt atcggattat
     4201 tgctgatcgt agtaggaagc atggtcacgg tctttatttg aggataaaga tggtaaaatc
     4261 tcatttatta aaaaaatatt tgtttgaacc aaacagtaag tacttataaa aaaatagcgc
     4321 tagaatttcc aacatctctg gaatttctag cgctttttaa ttgtttaaga tacaagtgaa
     4381 atatctgtct gcatcaataa tttttcattg atccattgac taaaatttac gacatgattg
     4441 aagttaccac cgattagtat ctccgcatga ttgtctttat aaagatcgta ttgtaaaaga
     4501 cggtcttttt tatttgcgta aacatcgtta tggtactgga ctaattgttc tcggatttga
     4561 ttgattgctg taaaatcacg atactgaagg ttgaaacgca tcgtgattgg tgcatttagc
     4621 atgaatcgct gcatttcttt tgctgcttcc tctgcttgat ctccgtatag gaactcaaaa
     4681 agattacgtt cagaaaattt ccattgcccg ccaacctttg ttccttttag ttttccttca
     4741 tgaagataat ttctgataga acgtgtagta acacctaatt gttcggctaa ttcatttact
     4801 gagtaaaaat ctttcaatgt tcaccctcct atgtgaatgg ttctactgac ttagttgata
     4861 tttgcttaat cacgaaatgt agaaagaacg attttaatta aggtcattgt aaaaatcaag
     4921 tcagaaaatt ctaacttttc tttctctaaa gaaattcgtt ctctcctaaa gtgatattat
     4981 atttaaatat acctgaaaat aatgggcagt attatgaaag atcactgata ttttctcgta
     5041 ttttttcctt attcaaaagt tttgcaggaa aattgctgaa aaaataattt tactaagtgg
     5101 aaaattcaaa gccattcaat cagtgatttt tttcagcaca agaaagactc cttataaaaa
     5161 tagcagatac tttgtcaatt aagtgtttaa ccagggatga taaaaaggat aggattgaac
     5221 aaaacctgta taagctgatg aatagtttcc tcgccagtaa aaggtttatt cgtataaagc
     5281 tttatcgcca aaatttatca tgaccttttt tatcttcaat attaaatgaa atcaattgtt
     5341 caatcaattc ccagttgatt acacttttcc aaggaacttt gatgacgtta tccgtatata
     5401 cgtagcctgc ttcttggata tttttttcaa gggcacgaat cgctgctttt tccggtgcga
     5461 tagaaaaatg atttttagca aaactaaatg caataataaa ggtaccatgg tcagtaaaca
     5521 taggttggtt ccattttaca gttgtgtcta gctggggata cgtttctgtt atcttagcaa
     5581 aaaggtcttt gatcttttgt tgatgttctt tttctttgat ctgattgatg atcggctgaa
     5641 atttttccat tctaaaacgc ctccattaag tggtttgatt tattttacca gaaaaacaat
     5701 agttatgtat aaaaaacgaa caaaacacaa gtaatgttgt aaccgctata tttatatgat
     5761 aagatgtctc aaataataaa atagaaaaga gacataaaaa tgattgactt ggcaaagaaa
     5821 ttcttacacg aaaatatata ttttgagttg tatcatcatc gagccattta tacaaatgaa
     5881 gatgctttgg taataaagaa agaacaagga tttacaggta cagagaccaa aagcttattc
     5941 ttaaaagata aaaaagataa taactacgta tatctgactt ttactacgaa acaaacggat
     6001 ttcaaagcat tagggaagat cgtcggtaaa aggctttcta ttgtttcatc agaacgcatg
     6061 gaagaacaaa cgggacaaaa gcagggcgct gtttctccgt ttggttatga aaaacagatt
     6121 ccgattatta ttgatgaaga gctcttatca aatgaaaaac ttgtttttgc accaggtaga
     6181 ccagatcaaa cgatggtcat ccaaacggtt gacttaaaca aaatcatcca gctacttgag
     6241 atgccgatat ttttgatgcc ggcaaacgaa aaaaacaatt aaccaagtaa aatagtgcat
     6301 acttggaggt gaagcaacat gataagttat ccattattag aaagaaatgc aacgattggg
     6361 gttactgcac catcctcagg cgtatcagag gaacaccatt cgttactcga atcagcttta
     6421 aagagaatgg aagaaaaatc tttccaagta gttactggaa agacgctttg gacgcaagat
     6481 aaggaaaaat caggtccagc aagaaaacga gcagatgaat tcaatcaaat gatgcaagat
     6541 gaaaatatcc aaatgatctt tccgccttgg ggaggagaat tattgattga gattctcgat
     6601 tttgtcgatt ttgatgctct acaaccaaaa tggatagtag gttattcaga tattagtacg
     6661 ttgctacttg ctgtcacatt aaaaacaggg atagccactg cacatggaac caaccttatt
     6721 gatttaagag gagagtactt ggatgaaacg acagcaatgt gggaaagtgt actgaaaaca
     6781 agagaaggag aatcgattct acagcaacct tctgaaaagt atcaaaaaga gtggccccat
     6841 gagcattcat catcttctgt tttccatctc actgaaccaa caaattggca atcagtcagc
     6901 ggagatgcag taaggatgaa gggacgatta ttaggtggct gtattgatac gatcagtcat
     6961 ttaatcggta ccccttatgg agatgtccaa tcttttaaaa gaaagtataa tgatggaaag
     7021 ccgatcttgt ggtttctaga aaattgtgag ctatcgtcag ttgatttaag aagatcactt
     7081 gttcagatga aactggcagg atggtttgat ggcagctcgg ggatcatgtt cggtagaagt
     7141 gcagcagagg atttgttaga ttataaaatg aaggatgtat atgaagatct gtcaaaggaa
     7201 ttaaaactac cgatcttgta tgatattgat tgtggtcatg taccgccaca actgacattg
     7261 atcaatggtg cctatgcaga agtagcggta gaagatagaa aagcgactgt tttgcaaacg
     7321 tttatttaaa aaattttttc ctgccttgga gtttatcaaa gaccaacaaa tctaaataga
     7381 aaagaaaatg aaagaagctg acagtatttt gccagcttct ttttgtatga tctattagaa
     7441 attttaatta tccataacta tcacattgat cagctgtttc acatcatttg gattacctgt
     7501 aggaatagct actacagatg cattatcaat aatttcttga gttgtttctc taatgaacgc
     7561 tggataagat gtttgatctg caggcatttg ccaatgtgaa ggaactggat aactagtgat
     7621 tgtcccgtca tggttacttg aatacactaa aactccctgg taactgtatt tcccagtgag
     7681 cataaccaca tctttcggat agactgcact ccctgcatca taaggggcga taggtgttcc
     7741 tgcaggataa tgaataacat gtagttcgaa atcacccgat gtatctctaa aagtattacc
     7801 tctaattgct agccaaacac gtgcgtactc aatttgtaga tcagaatatc ctgctaacgg
     7861 atctattgac tgagaagact gtgaggaagt tgtgtctatt gttgaagaag aaatatttgt
     7921 ctcaactgta ctactagatg tagaagaagt attgggctct gagactttac tgcttgttga
     7981 agatgtattc gtttcagtag aagctgattt ctgagtactt gatttttcag ctgaactggt
     8041 ttgtttgatt gaacttttgt ctgtggtcgc agatggatca ctattttctt tactaggaga
     8101 cgtacatcct acaaggaata aagagaccaa caataaaata aaacctaact ttttcatata
     8161 aaatacctca ttattttttt aataccagta tagcataatc ctgaattttt gaatccgtaa
     8221 aaccttgtc
//
LOCUS       DAZKKW010000101         8144 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.103, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000101 DAZKKW010000000
VERSION     DAZKKW010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8144
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.103"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            168..1001
                     /gene="msrA"
                     /locus_tag="ACG8PO_002385"
     CDS             168..1001
                     /gene="msrA"
                     /locus_tag="ACG8PO_002385"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFQ0207671.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQVERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            1081..2070
                     /locus_tag="ACG8PO_002386"
     CDS             1081..2070
                     /locus_tag="ACG8PO_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFQ0207672.1"
                     /translation="MYIADSARYEKMEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQ
                     REILRGAFDMGITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMW
                     EGPYGEWGSRKSIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGK
                     ALYIGISNYNGEDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGA
                     ITFSPLAQGLLTNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQS
                     LAQMALAWNLGQKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
     gene            2359..3057
                     /locus_tag="ACG8PO_002387"
     CDS             2359..3057
                     /locus_tag="ACG8PO_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HFQ0207673.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            3207..4523
                     /locus_tag="ACG8PO_002388"
     CDS             3207..4523
                     /locus_tag="ACG8PO_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFQ0207674.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            4540..5955
                     /locus_tag="ACG8PO_002389"
     CDS             4540..5955
                     /locus_tag="ACG8PO_002389"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFQ0207675.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            6419..6739
                     /locus_tag="ACG8PO_002390"
     CDS             6419..6739
                     /locus_tag="ACG8PO_002390"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0207676.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            6765..7082
                     /locus_tag="ACG8PO_002391"
     CDS             6765..7082
                     /locus_tag="ACG8PO_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFQ0207677.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LNA"
     gene            7111..>8144
                     /locus_tag="ACG8PO_002392"
     CDS             7111..>8144
                     /locus_tag="ACG8PO_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFQ0207678.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLV"
BASE COUNT         2684 a         1321 c         1726 g         2413 t
ORIGIN      
        1 tttcatttat aaaataaatt gattaaaaag gagaatctgt cgataaggat tctctctttt
       61 ttatacgaaa aatctgtagg gggatgaagt aatcgcttat ttcctcactt tatgataaat
      121 tacagctaaa gtcactagct tgtcttcttc ggaaaggagg agaatctatg gatgaggaac
      181 aaatattcat tcaaacactt tataatctga ttttgaatcc gaatacacga gattgggaac
      241 gaaaagtatt gattcagaca aagaatgaca taagagaaaa tatatccgtt aaagaacagt
      301 tatcaaaact agaagcaaca ctacgtccat tagctatccg aatgaatctg acgcctgatg
      361 taatggactt ttatttattg attacggaag gatttgataa ggagcaaaaa tatgactttt
      421 ccaagcatgc catgcaagat gctgactatc aagaacgtgc agtatttgca gggggatgtt
      481 tctggtgtat ggtcgagccg tttgaatcga aaaaagggat cctatctgtt ttgtcaggat
      541 acacgggcgg acatgttgaa aaaccaaatt atgatcaagt aagtggtggt tacacaggtc
      601 atgtggaagc tgtggagatc atttatgaca caagagaaat aagttatagc gagttgctga
      661 caatctattg gcaaattact gatcccacag atacatttgg acaatttcaa gatagaggga
      721 agcaatatcg tccagtgatt ttttatcaag tcgaacgaca aaaagaatta gcggaacaat
      781 caaaacagaa actagacagt tcaggaactt tccaccaacc aatcgtcaca aaaatcgaac
      841 cagccggtac gttttggcca gcagaaaact atcatcagca attttacaaa aaacaaccga
      901 aacgttataa aaaaatccag caagcacgta atcaatttct gatctatcaa cgagtaaaaa
      961 acaaatggca aaaaaatata cgaaaaaatc attttgatta actccttttc tttaaaatgt
     1021 agtaaactaa aaatagaaaa aagaaagcgt tttaaaaaga tttgatgagg aggagaaaaa
     1081 ttgtatattg cagatagtgc acgttatgaa aaaatggaat atcgtaaatc cggtcattca
     1141 ggtttgaaat taccgatttt atctctcgga ttatggcaaa attttggtga ttatgatcct
     1201 atccataatc aaagagagat tttaagaggc gcattcgaca tgggaattac ccatttcgac
     1261 cttgcgaata attatggagg ccctgctgga gctgcagaga aaaactttgg gcgtattttt
     1321 agagaagatt ttcaagccta ccgtgatgaa ctgattattt ctagcaaagc aggttatcat
     1381 atgtgggaag ggccatatgg ggaatggggc tctcgtaaat ccatcatttc aagttgtgac
     1441 caaagtctcc aaagaatggg gctagattat gttgacatct tctatcatca tcgcccagac
     1501 cctgatacac ctttggaaga aacagcagaa gctttgatgc aacttgtaag acaaggaaaa
     1561 gcattatata ttggtatatc caactataat ggagaagata caaaaaaaat gactgaaatt
     1621 ttaaaaagaa aggaagcacc gttcattatt catcagatgc gctacaatat gttttctcgt
     1681 gcactactag aagacgatct atctccagta ttagaagaag aagggctagg ggcaatcacg
     1741 ttcagtccgc ttgctcaagg gttgctgaca aatcgttatc tacatggtat tcctgaagat
     1801 tctagagcac atcgtaagga aataccattc ctttcagaag aacaagttgg atcaactctg
     1861 gaaaaaatca aagcactgca aacaatcgcc gtatcacgag gacaatcatt agcgcaaatg
     1921 gcccttgcat ggaatttggg acaaaaatct gttacaagtg tactggtcgg agcaagtcgg
     1981 ttaagtcagt tacaagaaag tgttcgaatg atggataatt tagatttctc accagaagaa
     2041 gaattgagaa tcgaccaaat tctagaatag aaacgatgta atcaaacttt ttatccatat
     2101 attaaaaaaa acaaaagatg acatcaagaa tcgaatgaag atcgtctgtt attgaacatc
     2161 atattttttg tcgttcaaaa taaaaatgat cgaaggctag gagtgaattt tatcctagtc
     2221 ttttttatgg taaatatctt cttcgtttgt atgtctatga acggattaat caattttgta
     2281 tacaaaaaat catcagatga ttcaaacgtg ttataataat tacgaaaaaa tacatcagat
     2341 gaaaaggagg tatgtggttt ggaagaaaat aaaaccctag tagaagtaac tactgacaag
     2401 atccttagat atttaaagga aatgcagttg caagtaggag ataaactgcc gaatgaatat
     2461 gaattgtccg attatttaga ggtggggcga agcacggtgc gagaagctgt gcgggctcta
     2521 gcttccagga atatccttga agttcggcaa ggagcgggga catttgtcag tgataagcga
     2581 gggattgcca atgacccact cggtttttct ttgattgaag atcaggatcg gatgatacga
     2641 gacttatttg aactgagata tttattggag cctaagatgg caggtttggc agcggaatat
     2701 gcaacgaaga atcaaatcgt ccaaatgaaa aaattaacgc aaaagattga aacttctttt
     2761 cgagaaggca cacaagaaca tgttcaatta gacatcgatt ttcatacgat gattgcagaa
     2821 gctagcggaa acgtagcctt gctgcatatt atcccaatca ttaatgaatc aattgctttg
     2881 tttaataaaa attatgatat ccagcatctg aaaatcgaga cgatcgatat tcatacagag
     2941 atcgttcagg ccatcgaaag gcggaatgca cttgctgcgt ttgatgcaat gactgtccat
     3001 atggcaataa atcggagaga attacagaaa ataataggag aaaaagaaac taattgacag
     3061 ttcatctgat gtattttatg ataaatacat cagatgaatt tttgttttat atttggtaaa
     3121 aaagaggagt aaatagtttc tgttcatcga aatgtaagcg ctgactaata gacaaaaaga
     3181 gatgacgtca aggagaagaa gcaggaatga ggggatctgg aatgagtttt atggataaat
     3241 tagccaatgt tttagggaac gtggcaacga gaatcaataa tttaaggtat atcatggtga
     3301 taaaaaacgc atttgctgca ttgattccgg taattattac gggtgctttt ggcacattgt
     3361 tttcagcaat ggtctttgac tcggaaaatg gcttagcaca gataagtttc ttgcgttttt
     3421 tagaagaatt aaagcctatc gcatctgcca tcagctatgt cacactaagt ttcttgacca
     3481 tctatgctgt ttttctgata gggatcgaat tagccaagct gaataaagtg gatggtgtat
     3541 tccctggtat catcgcagtg atgtcttatt tatctgttaa tccaactgtt tatgaattag
     3601 tgaatgaaaa tcagactgtt atagtagaaa acgtattggc taaacaatat acagatacga
     3661 aagggttatt tttgggaatg ttggtctcca tcctttctat tgaactttat tgctggttga
     3721 ggaaacagaa gaaactacag ctgagaatgc ctgataccgt gccaacgaat gtttctgcta
     3781 gtttttctgc tttattcccg acgattctga ctgtatcggg tatcgcggct gcgggattta
     3841 tcatcaaaga acttacagga atgtaccttt atgatatcat ttacaatgtc gtacaacgcc
     3901 cattagaagg tgtcgtacaa gggttaccgg gtattttgct attgatgttt attgcgcaag
     3961 tcttttgggt gattggtatc catggaaatc aaatggtgaa gccgatacgg gaaccgttgc
     4021 ttttagcggc aattgctgta aatacggaag cttttgaagc tggaaaagaa gtaccgaaca
     4081 ttattactat gccattttgg gatatgtata tgagtatggg cgggtctgga gtcacgatcg
     4141 gtttactgat tgcagtattg ttagttggaa aacgggaaga tatgaaacaa atcaccaagt
     4201 tatcccttgc tcctgggatc ttcaacatca atgaaccagt tatttttggt atgccgatca
     4261 tgctgaatcc aattttagct attccgttta tcttaacgcc tcttatcact ggaacgattg
     4321 gttattttgc aaccgcttta ggatttgcgg cgaaagcagt tgtaatggtc ccttggccaa
     4381 tgccgccgat cgtcaatgct tatctggcaa cagcaggaaa tatcggtgca gttattacgc
     4441 aacttgtttg tattattgtt tctattttga tctatctgcc atttgtaaaa gtagcaaatc
     4501 gtgtggaaaa tgatgggatc taaaaaacag gaggaaatca tgaaaattac tattccaaaa
     4561 aattttattc ttggtgctgc atcttctgct tggcaaacgg aaggttggaa aggaaaaaaa
     4621 gaaggacagg attcgtttat agatagctgg tataaaaatg aacattttgt ttggcataat
     4681 gggtatggcc cagccgtagc aactaatttt atggaacaat accagcagga tgtggatctg
     4741 atgaaatcca tccatctgac acattatcgg acttctatca attgggctcg tttttttact
     4801 gattatgaga actgtatagt agatgaagaa tatgctcagc atataagtga aatgatcgac
     4861 tctttgatcg aagcagacgt agaaccgatg atttgtttgg aacattatga agtcccagct
     4921 tttcttttgg aaaaatatga tggctggtct tcaagaaaag tagttgattt ttatgctaag
     4981 tatgctcaaa ttgcctttga tcgttacggt gatagggtaa aaaactggtt tacattcaac
     5041 gaaccaatcg ttccccaaac gcgttgctat ttggatgctg ttcgctggcc gcatgaacaa
     5101 aacacaaaaa agtggatgat gtggaattat cataaagttt tagcaacggc taaagcagta
     5161 gagatttacc gcagcggaaa ttatggtgga aggattggct gtattttaaa tcctgaaatg
     5221 gtctatgctc gatccaattc tccagaagac caaaaagctg cttatatgta tgatttgttt
     5281 tataatcgag tattttttga tccgatggta aaaggatact acacagaaga gttcctggag
     5341 ttgtgtcaaa aacaatcgat ttatttcgat cctgcaacag aggatttaga aacgattcag
     5401 catcatacag tagatttcct tggaatcaat caatattatc ctaaacgcgt tcgcgcccct
     5461 cgttatcaat ggaatgaaga gacacctttc catcctgaac gatattatga agtctttgat
     5521 ttacctggga agaaaatgaa taattccagg ggatgggaaa tctatccgaa aatcatgtat
     5581 gatatagcca tgtatctaaa agaaaattat cataatatcc cttggctgat aacagaaaac
     5641 ggtatgggaa gagaatatga agaacagtat atggatgaga aaggaatcgt gcaagatgat
     5701 taccgtatcg aatttatcgg acagcatttg cattacttga tcaaagcaac agaagaaggc
     5761 gcaaattgcg aaggctatat gttatgggca ttcactgact gtgtgtcacc aatgaatgca
     5821 tttaagaatc gatatggatt agttcgaatc gaattgaacg atgaacgaag cagaacattg
     5881 aaaaaatctg cttcgtggta tcagactttg attgataaga gagaattgga tttgcctgac
     5941 gaaatctata aataaataga agttttttgt agatgttcat tgtatttact ggttatttga
     6001 aataaaagag gttgtgaatc cagcgttttg agctaatacc cagggtcagc tgattgaaca
     6061 ccgtttattg agaggttgtg aaaaagctgt cctgcatcaa gtaataagaa tagcaaagta
     6121 aaaacaacat cttatgtttt taacttggtt ggacttatta ccgcagatgc aagcttttga
     6181 ataccgttta tttgaaaaaa gttgtgacaa gttttgtgtc acaacttttt ttgtgcacgt
     6241 taaaagaaaa aaggtaataa aaattaattt gtgtgtttag tgtgcgcttt cttttttagt
     6301 gtgtatgcaa cttggtatag accttaaatg tgatgatatc aactttttaa agcgctttta
     6361 aagttggcac gctacttgct tatatacaag tgtaagtata gaaaggaacg tgtttaaaat
     6421 ggaaaagaaa acaatcatgc ttgtatgttc agctggaatg agtacaagtc tcctagtcac
     6481 aaaaatgcaa aaggcagcag aagcaaaagg aatggaagca gacatctttg ctgtatctgc
     6541 ttcagatgcg gataatcaac tagaatctaa aaatgtagac gttttacttt taggaccgca
     6601 agtacgcttt atgcaaaatc aatttgctga aaagctagca cctaaaggca ttcctttaga
     6661 tgtaatcaat atggctgact atggcatgat gaatggtgaa aaagtactcg accaagcaga
     6721 aaaactaata aacaaataat aaaaattatc ggaggataca ataaatggaa gatcaaaaaa
     6781 atctagaagc catcatgggt ctgatcatgt ttggcggaaa tgcaaaaagc gatgcaatgg
     6841 aagcgattgc tgctgcgaaa aaaggtgatt tcactttagc agatgaaaaa atcaaagatg
     6901 cagaggaatc tttagtccaa gcacatcact ctcaaacaga gatgctgaca caagaagcac
     6961 aaggaaatca cgttgaagtc acgcttttga cggtccacag tcaagatcat ctgatgacat
     7021 ctatagcttt tacagattta gcaaaagaga tcattgatct gtatcgtcga ttgaacgcgt
     7081 aatctacagt aaagaaaaga gggaagattt atggataaat ttgttgcatt tatggagaaa
     7141 cattttatac ctgtagcatc aaaaattggt gcacaacggc atttagttgc gattcgggac
     7201 tcatttatgg tgagtatgcc gttaatgatc ttaggagcat tagcagtaat gatcaataac
     7261 ttaccgattc ccgggttcca agaattgatg aactcaattt ttggtggtga atcatggaaa
     7321 ggattcgggg ctgctgcttg gaacggtaca tttgccattc tttctgtctt gattgctttc
     7381 ttgcttgctt atcatttagc taacggctac cgaaaagacg gtgtttccgc aggtgtgatt
     7441 tctttaggct ctttcttcgc tttaggcgga gcattaggta tgagctcaaa cggtttgttt
     7501 attgctatta ttgttgggat tatttcaaca gaaatctttg tacgtttaag cggaaatgaa
     7561 aaattgatca tcaaaatgcc tgatggtgtg ccaccggcag ttgcaaaagc atttgcttca
     7621 ttactaccag caatgatcac tatctctgcg tttgctttag ttgctgcaat ttttgctgca
     7681 ttcggtgttg atgatatcgt aggttctttc tatacagtgg tccaagaacc atttatggga
     7741 cttgcaaact cttatccatc tgccttactg cttgcattta tcacaccatt cttatggttc
     7801 tttggtttac acggagcgaa tatggtcgat cctttgatgc aaacaatcaa tgcgccagcc
     7861 attgaagcaa acgttaatgc aatcgcagct ggacatagcg caccgtttat cgtgaacaaa
     7921 cctttctttg acagctttgt caacttagga ggaacaggtg caactttagg tttactgtta
     7981 gctatttatt tagtaggacg taaaaacaaa ccatatatgg ttgtaacaaa cttgtcgatt
     8041 gcgccaggtg tcttcaatat taatgaacca acgatgtttg ggttacctat cgtgttgaac
     8101 ccgatcatgt tcattccatt tattttgaca ccaatggtct tagt
//
LOCUS       DAZKKW010000102         8118 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.104, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000102 DAZKKW010000000
VERSION     DAZKKW010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8118
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.104"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1275)
                     /locus_tag="ACG8PO_002393"
     CDS             complement(<1..1275)
                     /locus_tag="ACG8PO_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFQ0207679.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIGDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQI"
     gene            complement(1416..2018)
                     /locus_tag="ACG8PO_002394"
     CDS             complement(1416..2018)
                     /locus_tag="ACG8PO_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HFQ0207680.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFVLEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            2263..2469
                     /locus_tag="ACG8PO_002395"
     CDS             2263..2469
                     /locus_tag="ACG8PO_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0207681.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            2781..3500
                     /locus_tag="ACG8PO_002396"
     CDS             2781..3500
                     /locus_tag="ACG8PO_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFQ0207682.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(3581..4264)
                     /locus_tag="ACG8PO_002397"
     CDS             complement(3581..4264)
                     /locus_tag="ACG8PO_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family protein"
                     /protein_id="HFQ0207683.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            4482..6668
                     /locus_tag="ACG8PO_002398"
     CDS             4482..6668
                     /locus_tag="ACG8PO_002398"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HFQ0207684.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
     gene            7014..7424
                     /locus_tag="ACG8PO_002399"
     CDS             7014..7424
                     /locus_tag="ACG8PO_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFQ0207685.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            7438..7794
                     /locus_tag="ACG8PO_002400"
     CDS             7438..7794
                     /locus_tag="ACG8PO_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFQ0207686.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT         2968 a         1332 c         1524 g         2294 t
ORIGIN      
        1 tatttgtata taaatcgttt gagaaaaacg ctggttttca attgatgttt caaggaaaag
       61 ataaggattg gtctttgtca attcatccaa gataaacaag ccaaggcctt cgttttttgg
      121 atttgttttt gttgagaacc ctttttgttt caactggttt aacggtaatg tatttgttgt
      181 tgtattgatc acacgaatca tcacggaatg attcttcttg agtatggtca attcgatttt
      241 tttcttcttt gcagcactag ctccttcaat cgcattgtcc aagataatac caagcatacg
      301 tacaaggttt accgtatgtt tcgtatccag ttcgattggc tcaggaaaat gaagctggac
      361 gatcaaccct ttttcctgtg ccatcatgag ttttaatgac agtaaactac ggatctcagg
      421 aactttcata ttatccaaca tacttagatt ggccggaaaa aggttcatca tctggcgtgt
      481 aggtaatatc gtttgatcat aataagaccg taatccttct aagtcgcctt ctgaaatata
      541 gctgtgaaga gaaaaaagca gattttggta atcatgtttg aattctctca agtcttgata
      601 gtttttttct agatctttga tataatcctg ttgcatcctg acttgttgct ctaataactg
      661 atcagctaat ttcttattag agattttcca ataataaacc aagatgatca ggccaacaca
      721 cagatataga atgaaaaaac tcgtattcaa agctaagaca tcaggtgttt caccagaaaa
      781 acgggtataa aaaataatga tatagtatgt aaataatatc aatgtcccga tcgtaccgat
      841 catacggcgg tccacagtgt gaaaccaagt ctctaacgat ttttttaaac aaaaagctaa
      901 taaagcagat aaaaaagcgg ataagacaat gtgaaaatac tgcaatgaat ctcctggttc
      961 aataaccgaa gatttcaaaa caaaaaaatc gccgatgctt gttaagtgat ctcctaaaag
     1021 cgacgtcaat aacccaaaag gaagcgcgta aaccaattgt tgacgcgaag tcgaaaagaa
     1081 gaaaaaagta agcatcagca ttcctatagc aattagagca gtaaaaatcc ctaaaatcga
     1141 atagacccat ccaatcaaaa tacaagaaat cagaatgact cccgcaaaca gatatttgta
     1201 tcggatgtga tggtcaatca ataagataca aattgtgtaa atcaaatatt gcaacgctat
     1261 ggaccataaa aacataaagt acagctccca ttgattttta ccaaattata gcacaattgg
     1321 cgtattattt ttttaatttt tcagggattt tgaattcaga actcatacca aagcagcagc
     1381 cagatacaaa aaagtcagat aagttagtta attgtttaat gattgttttt ttcatgaaag
     1441 attcctccaa aattcatcaa gtagtttgta ataggaagca acgtagctat ttctaaaaca
     1501 atcccaagta gcaaataggt agaaaagtaa gaagctgaga aaaataacgt aatgagagaa
     1561 aaaattagaa acatgctgat cgccttcttt aataatgggg gacgtttctc gggttcaatg
     1621 ggattgattg ctgtacctct tgatccatag cgaagcagta acaaaaagca gctgattaac
     1681 aaaaagacca tcattgggaa ggagatagtc aattgcagat gtttcatcac caagggaacg
     1741 ccccataaat aaagtagtgt ccacagcaga caaacgaatt cagagtcgga atgcaatcca
     1801 taagcatatc tgcggatgca gatataagaa agaaaggtga ttcctgtttc cgcccatgag
     1861 cctgtcacca gagaaaagac tagaacgacc gctagttttc caagattatt caataaaact
     1921 tctaagacaa attttacttg gacatacacc atatcttctt ccgatttgtc tccgctgaat
     1981 aaggcttcta ataatttgtt cacaacttta tctttcatct catacgtacc tccaccacaa
     2041 attttatgct aaaaaaaata aaaaacgtct tttgtttcat aaaaagtggt tttgacatca
     2101 aaaccgtcat gatctcctaa aaaaatcttc gttttccaag aaccttacca aaaaaagtga
     2161 cagttttgat acgaaaagta ctttttatga caagtttgtg ctttttcgag tgatttatgt
     2221 taaaaaatta ctgaccttat aaaaggtgat ggagggattc ttatggaaaa tgaacaattg
     2281 tttgaagtcg gcgaaacata tgcatttaaa tctgatttat ttgaatcacc aataaaagga
     2341 aaaatagaaa aaatctatga aaatagtgtg atgatacgtg tcatgaaatg cgcagtagaa
     2401 gacaatgata ccgctcataa tctgcactgg cagttagttg tacgcaaatc aagtacaata
     2461 gaagaataaa aacagataaa atgttgagag cataaagagg ttgtgagtct cccgtttagc
     2521 tctgagttac aaggaataat gttaaaaaac agcttttcat gttttatgaa tattgccttg
     2581 tgaccgaggt gttggcagga gaatactgtt tatttgaaaa aagggctgcg acaagacttt
     2641 tgtcacagcc tttattttca tgaataggag ttacttttgg ctacttttag agaagtagct
     2701 tttaaggagc agataaagtc aaggctttta tttctataaa tgaagtagta aaatgaagaa
     2761 cagattttat tacaggaagg atgccgataa ttgtgatcat tgttgcagga acgatcggtg
     2821 cagggaaaag cacactaact gaaatgcttg cccaagattt agaaacaaaa cctttttatg
     2881 agaacgtgga agataatgaa gtattgccgc tattttattc taatccagaa aagtatactt
     2941 ttttattaca gattttcttt ttgaacaaac gctttttggc aatcaaagat gctttcagcc
     3001 atgatgacaa cgtactagac cgctccatct acgaagatag tatgctgttt catttgaatg
     3061 cagatttagg gcgtgtttct gaagtagaag taaagcagta tgaaggtctt ttagaaacga
     3121 tgctgaagga gttagaagaa atttcgccac aaaagaaacc agatctactg gtttatattc
     3181 gtgtatcctt tgaaactatg ttagcgagaa tcaaaaaaag aggccgggaa tatgaacagc
     3241 tagaacaaga tcctgaactc tattcctact ataaagaact caatcgacga tacgaagagt
     3301 ggtatgaaca gtttgatata tgtcccaaaa tagtgatcga tggggacaaa tatgatttcg
     3361 tagccgatcc ggcatgtgga gaacaaatcg tacaagaaat caagatgcgg gcaaagaaaa
     3421 tgacagaaga ggcagatgcc cagtcgatcc gctacactac aaataacccg aaagcaaagg
     3481 gaatctttac aactaaataa tataaaaact aagcaaattg ctcactcgca gatatttctc
     3541 tgtgagcagc atcttgctta gtttttttga ttcattgaga tcaaatagtg aaccgattcg
     3601 tatgataaaa ttgccataaa tgaaatgtct gagtgagggc catgatcaaa aataaaatgg
     3661 aaacgatcaa aatcgaatgg gcgccaaaca gatgatgaca gaaaacaagg acaatagctc
     3721 ctgcgataaa ggcaagaacg acacttagat agtggaaaaa tcgatcaaaa tctgcttttt
     3781 ctttagaaat acaataatca tataagttcg ttgctacccc ttttaaattt ccagttgtaa
     3841 ataaattagt gtaggcacgc ccgttgattt tatcaaaagc tacccactgg atcgatgcag
     3901 taaaagcaat caaaatcgtt actaatgcgg gatgctcatg gacgggtgtc aaactaacaa
     3961 agaaaaaaat cactgcttca taatatagaa caaacaagcg ccagaaatgt ttttcttttt
     4021 ttctaaaata atcaattaga tactttgtta gaagaatccc taagaaaaag aaaagggtag
     4081 acaatacttt tttaccaaca cttagccatt ctccgtcaaa tgcttggatg atcgatagaa
     4141 tgatattgcc agtttgagca gaggcaaaag caccatattg gatataagta taactgtcca
     4201 tggctcctcc gataaatgtc agcaaaagtc ctattgtacg cccctcgtga aaggaagtct
     4261 gcattttttt catcaccttt tatgaatatt tttgtcttcc ttatcttagc ataaacgatt
     4321 gaaaaaaagg aaaaattttc agaaaactaa aatcatatcg cttcccattt tcatttttag
     4381 aaaggtctgt tagtataaag aaaaaataga caacgaaact ttaaataacc aacttgaaat
     4441 aaacaaattc aaagaagaaa gatgggaagg aaatcaatca gttgaatgaa aaagaaattc
     4501 tccgattaca aaaagaattg aaacgctggg agaacaagta taaaaagctt caacaagtct
     4561 ttcggctaca agaagaaatc attgctacct atgaagcggc ttatggcgaa gcacccggac
     4621 ttattgagga agaagtacaa gtaaaagaaa gtgaaggaaa cagctctttc tttaaaatga
     4681 tggagaaacg ttcctataat cgcttcaatc aagaacaaaa agcagctatc acatatgata
     4741 tgaagaaaca tcttcgaatc atagcaggag ctgggagcgg caaaacgcaa acaatctgtg
     4801 caaaagctgt ttatctgatg acgcaaaaac aagtagacga agaacgcata ctaatgatca
     4861 cttttacaag gaacgctgca aatgaactaa aaaaacgagt agataatttc agccagagaa
     4921 aaaccgctgt ccatatcgga acattccata gtatcttttt taggatatac aatgaaattt
     4981 gtcgaaaatt tcctgaagta gcaatgcaag gaatacaagg agacttttca aaggatactg
     5041 cacaaaaagt gaatgctgtt ttgcaacagc tgatacgtaa atataatctt tatatatttg
     5101 atcaatatgg agaaaaaacg attgcttctc ggttagacta ttggcagaat atgaaccttt
     5161 cagtagaaga aatggttcag ttagttaaag aaaaatatga ttccattgat aagaatgcac
     5221 gacagccgat atcagaacga ctgtatggat tgctcacgga actccaagaa caaaaaagaa
     5281 gccagcaatt gctagagttc aatgatgtac tccaaaatct gaaaagcgct ttggagaatg
     5341 aggagatcca gcattatatc ggccaaaaat atgattatct attcattgat gaatttcaag
     5401 atactaatcc acttcaatgg gaaatcgtga aacggatgac aaaggaaaac aggatcaagt
     5461 tgattgttgt tggagatgat gatcagagta tctatggatt cagaggatca gagccagcat
     5521 acatcaagaa ctttgaaaaa gaatatccta ccaaaactct tttcttatta actaattatc
     5581 gttctcgggc agctattgtc caaggagcaa atagattgat cagctataat aaaaatgatc
     5641 ggataccaaa ggcgatgatt ccagcacaaa aagaagcagg tgttatggaa gcctatttgt
     5701 tttctgatac taaagcagaa agtcagtggc taatcaaaca aattcagaca tttattcaaa
     5761 aaaatgggaa atataaggaa tcaatcatat tataccgatc agcttcacaa acgcagcagc
     5821 ttgtccaatc tctgctgaaa acgaatattc cttttgtact ggaagcagat agtccatatg
     5881 aaggtatctt tggtatcaag tctttccaac atttctatca aaaaattgtt cattggcaaa
     5941 cagcactgaa tctgcaaaag aaaaatcagg cctaccagca attgctccgg cagctgatgg
     6001 cagactgcta cctgaaaaaa agtgaatgcg atcaatactt ttctcctaaa tatcaaaata
     6061 tagccgttgc agattatatt ttgtcgataa gccccaattt aaaatcgaaa tctgcagaaa
     6121 tccgagagtt tgaggaagcg ttgcgtaaaa cagaccagca tcaagaaatc tattcgcttg
     6181 ttcaaagcta tcttcatctg ccacgtatag ctaaggaaat cgaccagagt gatcaagaat
     6241 ggatacgaga agaagtaaag cagcatgcta ctttcagaag actccaagcc ttatcgcaag
     6301 aaacccaagc gacagctaaa gaattaaaaa cccgcttgat tgcttacaga caaggaaaac
     6361 ttgatgcact atgtatccag agcattcaca aaagtaaagg tcttagttac cgaaatgtct
     6421 ttttgattgg ttgtaacgaa ggaagtttgc cttataacgg agcgacagaa aagaaagtaa
     6481 tcgactttaa tgaaataaaa gcagaaccag taacgacgat agaagaagaa cgaagactat
     6541 tttatgttgc gatgactaga gcaagaaatc gtctgtatct atgtgtacct caaatgaaaa
     6601 attcaagaaa attgaaagta tcgagattta tcaaagaaac cgggagtcgt gtgaaaaaag
     6661 gaaagtgata gaagagaggg tgtgacagaa gtggtcagtt tcaagaaaga agaaggaatg
     6721 tacgaaaatt gcttctcaaa tttttgtgaa atttcagctt attttcgaag aagctatttc
     6781 tggaacacag tttattcgga aaagggaggt gtgacaagaa ttttgtcaca cctcccttat
     6841 ttattttgaa aatgagaata ctattatcaa aatagagaat atatagaaaa attctcatta
     6901 ttcaaacgta aataatcagt tattttgatt ttttttatat tttattagct aataattggt
     6961 attttaaaag aaattactgt agaattggat tataattaaa aaagggtgag aaaatgataa
     7021 aagtatatac ttcgcctagc tgtacttcct gtagaaaagc aagagcatgg ttgcaagcga
     7081 atcaacttga atttgaagag aagaatattt ttgccgaacc gttaacagaa agtgagatca
     7141 aaaagatctt ggcagcgact gaaggaggag ttcaagatat tatttccact cggtcgaaaa
     7201 tttacgaaaa gctgggcata gattttaacg aattaacttt aaagcaaatg atcgtcttat
     7261 tcaaagaata tccttcatta ttgagacgac ctattttgat tgatgaaaaa agaatactaa
     7321 tcggatttaa tgaagatgag atccggtcat ttgtccctcg tgaaattcga tgtgttgcca
     7381 gaaatctgat ctatttacaa aacaataaca tcttaaattc atagggaggg agccttcgtg
     7441 gaagtgaaag tctatggatc agcgcactgt gtaacaagtc tgaaggcgaa acaatggtta
     7501 gaacaccatc aattgcctta tcaatttgtt gacttagaag aacgcgatct aagtacagaa
     7561 gaagcagaag aattatgcag ttttgaagaa gtgcaagcag aacagctatt tgcgacttgg
     7621 tctgaggctt ttcacaacat tgcgattgac ctttcctgtg taggaaaaaa acaactgatt
     7681 tttttatgca cacaacatac caatctcctc agacgtcctt tgattattat cgatgaagtt
     7741 ctatttattg gatacaatga gcagttgatg gaacagctta tcttaaataa atagacttta
     7801 actgttttaa tcaagtcact gagcatttca tacagttgta acagtcgaac acttagcatc
     7861 aagcgtgtta tgtgttcttt ttttgacttt atttttctac tatagagaca tgctcaattt
     7921 atgtggtcaa aattcaaatc taggtgggga tatagagtta aaaatatatt gacatgtgta
     7981 aatttacatg atatattcat ttctgttgtt ttaagtagca aagcaataaa atagtatttt
     8041 aaaaaacata taaaatagtt gttgacatgt tagaaatgac atgataagat aactgagttg
     8101 ttaaaaaaga accacttg
//
LOCUS       DAZKKW010000103         7722 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.105, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000103 DAZKKW010000000
VERSION     DAZKKW010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7722
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.105"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            175..1602
                     /locus_tag="ACG8PO_002401"
     CDS             175..1602
                     /locus_tag="ACG8PO_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0207687.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(1694..2059)
                     /locus_tag="ACG8PO_002402"
     CDS             complement(1694..2059)
                     /locus_tag="ACG8PO_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HFQ0207688.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPVDVQNK"
     gene            complement(2056..2421)
                     /locus_tag="ACG8PO_002403"
     CDS             complement(2056..2421)
                     /locus_tag="ACG8PO_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HFQ0207689.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(2613..2861)
                     /locus_tag="ACG8PO_002404"
     CDS             complement(2613..2861)
                     /locus_tag="ACG8PO_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0207690.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            2860..2988
                     /locus_tag="ACG8PO_002405"
     CDS             2860..2988
                     /locus_tag="ACG8PO_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HFQ0207691.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            3045..4739
                     /locus_tag="ACG8PO_002406"
     CDS             3045..4739
                     /locus_tag="ACG8PO_002406"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050151 - oleate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HFQ0207692.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            4839..5012
                     /locus_tag="ACG8PO_002407"
     CDS             4839..5012
                     /locus_tag="ACG8PO_002407"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0207693.1"
                     /translation="MQAFEHRLPRGYEKAALHQVIRAAKLKQLLMFLTWLDLLPQMQA
                     FEHRLPRGYEKGV"
     gene            5390..6523
                     /locus_tag="ACG8PO_002408"
     CDS             5390..6523
                     /locus_tag="ACG8PO_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HFQ0207694.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            6554..6874
                     /locus_tag="ACG8PO_002409"
     CDS             6554..6874
                     /locus_tag="ACG8PO_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhK family protein"
                     /protein_id="HFQ0207695.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(6903..7367)
                     /locus_tag="ACG8PO_002410"
     CDS             complement(6903..7367)
                     /locus_tag="ACG8PO_002410"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0207696.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(7499..>7722)
                     /locus_tag="ACG8PO_002411"
     CDS             complement(7499..>7722)
                     /locus_tag="ACG8PO_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFQ0207697.1"
                     /translation="VSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAA
                     VNLVLSILIYLPFVKVATIQERRKEALDA"
BASE COUNT         2531 a         1481 c         1380 g         2330 t
ORIGIN      
        1 aaattcgcaa tttatttttt aatagaatta tctgttttcc aaattttata acataaaaaa
       61 ccttttttaa cggcatttat atgctttttc agaaattaac tgatgatttc aaaaagaaaa
      121 catgtcataa tatttcccat attgtgcaaa aaaatacaag gcggaggctc tactatgatt
      181 gaagaatata ttgagaaaaa tattttaaga cagctgtttt tatgtgggca attctatgta
      241 aataaagaag tcaatctaga aaagctctcc aatctcctgc atgtttgcaa aaccacttta
      301 ctcaatgata tcaataacat aaaaaaagaa tttgaagaac agatcgctta tacacatcgg
      361 gaaaaggacc gttacacttt atatttttca gaacatatac ctcgatgcaa gatcatgcag
      421 caattgtctc aaaattcgtt gtttttaaaa acttgtctgc tttatttgga agaagatgaa
      481 cctgattatc ttcaattaac tgaatgtgaa ttcatctctg tcagcaaagc ttattcatta
      541 aaaaaacaag tattagctta ttttaatgac tgcgggatcg aaatcgaccg ctactcccct
      601 cgattcactg aaatggaaag acgtctgctg cttcttaatg tgtcgtatcg tttaggcggt
      661 tttaattctt gggagctacc tgaatcgttt tttgaacgtg ccgatcgttt cattgaatcc
      721 gtaacggaaa atagtggcag attttatgat aaagaaaata aagaaatctt atcgattggt
      781 tttgctatca gtttcctacg ccagcaagtg tgtgctgtga caatagattc aaaatttatc
      841 gaagaaatca aaaaacgtcc tatttacaat tatgtcgaat cagcatggga aaacactgat
      901 ttccaaacct attataaaaa agaagaattc gcctttattt tgacactatt caatctttgt
      961 aattatggtt tccattctta tcagttgatt gcggaagact ttcaacagct acaccaagtg
     1021 tttattgaca atactcccga aataaaagaa ctagttgcta cctttgaatc tcacttcaat
     1081 caagaattat tcggaaacca accgttcgaa cgtgcgctga ttcatctgat gcgttcggct
     1141 tgggataact atcaattatt catgccagaa aaattttatc ttttaaatga ggaacagaca
     1201 aatttatata aagaagtcca gacaattttt tccagctggt ccagccagtt accttatgat
     1261 ctacggttga atcctaattg catgcgggct tttgtgatcg aattgagcgg aattctccgt
     1321 ctgacaaaag aacatctgac tatctatatc gtcacaaatt cggatgttca ttatctgatt
     1381 tatcgggaag ccttggaagc tgtaactact ttcgattttc aagttgcacc aacaatttat
     1441 tcaagtatat ctgatataaa gaagtatgcg cagcagtcct caaaccgggt attatgtgaa
     1501 cgtacattat atacaccaga tgcggtccaa tatgaaaata ttattcctat ttcgatcaat
     1561 acgatcgatc gagctatcat ttcagctgtg caaaacaaat aaggggctgt gacaaaaatc
     1621 atgtcacagc ctcttatttg ttgaagatgc ccatttctgt aacaatctct tgatacacgt
     1681 caggctcaca acctcactta ttttggacat ctacgggtcc gatctcttta tttaaaaaat
     1741 aagaaatgat ggttcggata actaccaacg ccgctagttg gaaaatatcc tgcaaggtag
     1801 gtttgataat cgattcgatg atcccagctg aaacaagaat ttccaaactt agaagtatat
     1861 agcctcccaa catcttttta atagcattgt tctcgctgac tcggtcgctt tgcacttttg
     1921 tatggacaaa taaatatttc aaaaaatcct tgattgatag acaaacaccc caaaccaata
     1981 aacagatagc aaagatatca agtcccagta caagtttttc gaaatatgga agaagaaact
     2041 gcatcaactc tgtcgtcatc ctacctctcc tcttctttgt ttttctttgt cttctatttc
     2101 ctgattcaag aaataagaaa tgactgttcg aatgataata ataagtgcaa gcttcaaaat
     2161 atctgtccaa gtcggcttga taactgactc gatgatatca gcaacgacta gtacttccaa
     2221 gcttaacaaa atataacttg ctagcagttt tcttgtatct gcattacgag tattacgttc
     2281 tgcatagttt ttcttgacat gagaccaatc aaaaagtttg aatagaacaa gaacgatccc
     2341 gacaattaag ataccgatag aacaaatatt taaaatagca atgatccatt ctagaaattc
     2401 aattaaattt gagcgaaaca tatacgctac ctactttctt ttaatttgtt cctattcttt
     2461 tattgtaaaa gaaagttacc ttgttcgtct atttaaaagt tgttctaatg ataacggttc
     2521 gcttaaattt ttctttttca ctctcccatt tttctccctc catccataaa tcgtggaagt
     2581 atccaaaaca acgtagcaag ttaaaataag gcttagtccg tcatcagctt ttcaaagaag
     2641 cactgtctcg gtgctttttt tgtacccaaa tttctaaaaa tagaaccata cacctcctca
     2701 aatcttttct tcttacctac cctattttta tttacaggaa aatcagacac ttcttctttt
     2761 ttgtcaaaat ttcctttttc ttctcgtcct atcatttggc attctaataa aaacgaacta
     2821 tccttggata tagaaagaga aaacaaggag gcgtttttca tgagtaaaaa attaatcaaa
     2881 gtcggcatcg gtttaggttt gcttgccctt ggcgctgcat atctaggaaa aaagacagga
     2941 ctattcgaag atgacagtca tttatatgat gaattcgaat caatctagca tcctatttag
     3001 ttgaatcact acggacatac ttggaagaca ttggaggaat cacaatgaga aaaagtaaag
     3061 caataatgat tggtgccggt attgccaata tggcagcggc tgtttatttg attcaagaag
     3121 gcaaatggaa aggggatcag atcacctttt attcattgga tgatcacggt tcaaatgatg
     3181 gtgcaccgac cgacactgta gtggatgaat attggaataa aaaccatcca ttagaaaacc
     3241 aaaaaggcta tattgctcga ggcggtcgca tgcttaatta ccgtacctat gtagatttga
     3301 tggatctgtt aagtcgaata ccttctgtta ctgagccagg gatgactgct gaagaagata
     3361 cgagagattt tgatgcaaaa caccgaacct ttgacaaagc acgtttgatg gaaggtggca
     3421 aaggaatcat tgatggcgga aaactgggat tcaataataa agaccgtatt cttctgacta
     3481 aactgattgc tatgcctgat tcagaagaag aaaaactaga caatatcact atcgaagaat
     3541 atttcaaaga tgatccacac ttttttgaaa caaacttctg gtttatgtgg gaaacaacat
     3601 ttgctttcag aacacgtagt tctgctcaag aattgcgtcg ctatatgcat caaatgattt
     3661 atgaattcac tcaaatcgaa catctagtgg gcgtcaatcg aacacgttac aatcaatacg
     3721 aaagtattat gcttcctttg atcgagtact tgaaagaaca aggatgcaaa atcattttga
     3781 accgccgtgt cactgactgg gaattcaaag aaacaccgat gcaagatgaa atcactgtga
     3841 ctggtttgaa aattaccaat acactgactc aagaagaaga atacgttgtg gtcgatgaag
     3901 atacagcagt tattttcact aatgggtcca ttacagattc agctacttta ggagattacg
     3961 aaacaccagc tgtagaaaat atggattatg gtgcagcttc tagtctctgg aaaaaagcca
     4021 gtgaacgttt ctacaacttg ggaaatcctg acaaattctt tgctgatcga gatgcaagcg
     4081 aatgggtcag ctttaccctg acgacgaaaa accatttgtt gttgaatgaa atcacccgta
     4141 tcacgactca agaaccagga aatgccttga attcatttat ttcaaccaca ccgatcacgc
     4201 cgttaggcca aaaagatgtc aatatgtcta ttgtcgtgca tcatcaaccg cattttacgt
     4261 ctcagaaacc aaatgaaaca gttatttggg gttatttcct ttatccaaga cgacgtggcg
     4321 aatttgtgga taaacagtat attaaaatga atggaaaaga aatgctagaa gaactgattg
     4381 gacaactttc aaaagtagat cctggaccag tgaatattcg tgaaaaagaa acagaaattt
     4441 tcgacagcgt aataaataat atccctgtct atatgcctta tgcttccgcg ttgttcaata
     4501 atcgtgccaa aagcgatcga ccaaaagtca ttcctaaaca ttcgaccaat ctggccttta
     4561 caggagaatt tgtcgagcag ccttatcaga tgatctttac tgaacaaagt gctgtacgtt
     4621 ctggagaaat cgctgctttc cattttgcgg gtattcctat gtcccgtctt gtcaaaacac
     4681 cgcgttacga caaagatatt cctactctag ctcgtgccgc aaaaaaaatg tttgaataat
     4741 caagaggttg tgaaaaagct gccctgcatc aagtaataag aacaaccagg ctaaaacagc
     4801 ttcttatgtt tttgatttgg ttggacttat taccgcagat gcaagctttt gaacaccgtt
     4861 taccaagagg ttatgaaaaa gctgccctgc atcaagtaat aagagcagcc aagctaaaac
     4921 agcttcttat gtttttgact tggttagact tattaccgca gatgcaagct tttgaacacc
     4981 gtttaccaag aggttatgaa aaaggcgttt agtttcgagt atgaagaaaa attttgtgaa
     5041 aatggttcct cacgtttttg cataaatttg acttaatacc gagaaactga tcccttctgt
     5101 cataacctct ttttatttat gtctgtttct ttttgaaaac aactattttt tcaacgttta
     5161 ctctcttgcc aatccaacat catctaacgg atacaactat ttcaatgatt accattacac
     5221 taaaaaatcg gaaacacacc tctccgaaca caaatacata agactataga atgtttcatt
     5281 taagaaaaaa agcgtttcat tatcaaaaag gtaagatctc ctttaaaatg aaattatctg
     5341 tagtactaat aatgcagaca attttatgaa tctgaaagga gcatctccaa tgaaagcagt
     5401 cacttggcaa ggaaaacaga aaatggaaat cagaaacgta gatgatccaa aaatattaga
     5461 accaacagat gcaatcatcc gtatcacagc aacagccatt tgcggctccg atctgcatct
     5521 ttaccatcat ggtgaagcag ttttggaaaa agggtatgtt gtaggacacg aaccaatggg
     5581 aatcgttgaa gaagtgggat ctcaagttca aacattgaaa aaagggcaac gtgtggttat
     5641 cccttttaat attagttgtg gtcattgtca tttctgcctg aatcacatgg aaagccaatg
     5701 tgatgaatca aatccagatc agttatatgg cgggcttttc ggatttggca agttgaacgg
     5761 taatcattgg ggtggtcaag cggaatatct aagagttcct tatgcagact ctacttcatt
     5821 tgttgtacca gataatgatc ttcctgatga aaaagtttta tttctatcag atattttgcc
     5881 cacagcttat tggagcgttg aaaacgcagg agtaaaaaaa ggcgatacag ttgtgattct
     5941 aggttctggt ccagtcggtc tattcgctca aaaattcgct gttatggctg gagcagagcg
     6001 cgtaatcgca gtagatccag tacagcaccg tctagataaa gcggctaatt acaatggggt
     6061 tgaaacttat ttcttagaag acacggcaca agctggaaca gaattgtatg aactggctaa
     6121 aggtggcgca gatgtcatta tcgattgtgt aggaatggac ggattagagc ctgtcaaaga
     6181 aaaagcgaaa aatttagtaa gcttacaaag cggcacaatc tctccattac agatggcatc
     6241 gcaagctgtc aaaaaatttg gaacagtcca agtaactggt gtttatatga ctccagcttc
     6301 ttcttacccg ctgcaagaat tttttatgcg ggatatcgct gtcaagcatg gacaggcacc
     6361 aattatccat ctaatgccaa aaatttacgc aatgattgca gaaggactat tcgatcctac
     6421 acaaatcatt actcattcgc tgccactatc agaagcagca caagcatatg agatctttga
     6481 caaaaaagaa gataaaaata taaaagttgt tttgaaacct tgaattcagt aaaaaataca
     6541 ggtgattttc tttatgccca aaatcaaacg aaatacccat gagatcgaaa taggaaaaga
     6601 agaagatatc gaaatacgag gacgtcgctt ccgcttctat ttccaaaatc gttacacatt
     6661 gatctcactg gctgtcgatt tattaaccgg gattttctat atttttggaa gccttgcttc
     6721 cttaactaaa atccccgatc gatatggcac ctatttttat ttggctggtg ctattttctt
     6781 gacgattcgt cctattctca gaatcgtaag gaacattttt atttacgacg aaaaaaaagc
     6841 aatgaagaga acaagcaaaa aaaaagaggc atgagaattt ttcaaatctc atgcctcttt
     6901 tttcacccta tcgtatcact ttgatacaga cgagcggtcg tcccactttc ttccttgacc
     6961 attgtcaaaa atgtccactc gtatcgttca taaaacgtgg tatgatctgt aattaagtaa
     7021 agtttttcgt acccaagttg atgcatttct tttctgattt ccgctaatat ccgcttggct
     7081 atcccctgct tacgcttttc cggttcaaca aacagggcac aaagatttgg tgtcagatca
     7141 gggcggttat ggaagtcgtt ttcaatgacg cccgctcctc cgacgatggt ttctttttca
     7201 tctaagacca cataccattg aggaatactt tccttattag cgattccagt acgaatactt
     7261 tcctcataca caacagcagg aactccccat tttgatgaaa accaattcgc gctttgtttt
     7321 actaattctg ggtgttctct taatttgatc cagttatatg taaccaatgt ttaaacctcc
     7381 gactaaaata aaacgtttac aaaaattata caagtcttta tgaaaattag caatgacaac
     7441 aatagtgatg taaaaaaaaa aacggaaaac aatcacttgt tctccgtttt ttgattaatc
     7501 aagcatctaa agcttctttt cttctttctt ggatcgtagc taccttgacg aacggtagat
     7561 agatcaaaat agaaagaacc aaattgacag cggctagtac accacctgca atactttttg
     7621 ttgccaatac accgccaata attggtggag ttacccatgg aggcataacg gtagctgctg
     7681 gcactaaacc tgtagaagtc gcaaagtaag caacactaac ta
//
LOCUS       DAZKKW010000104         7599 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.106, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000104 DAZKKW010000000
VERSION     DAZKKW010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7599
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.106"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(136..426)
                     /locus_tag="ACG8PO_002412"
     CDS             complement(136..426)
                     /locus_tag="ACG8PO_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0207698.1"
                     /translation="MGEKKIKGAGILLVMSKALFLLPCTFMFIVLCYFKRRKKWCWID
                     VLLFLPFFPLIYFLEEIWDDMACDSAFLFSHSLGLMISLTIFAIVLFFLNKK"
     gene            complement(544..804)
                     /locus_tag="ACG8PO_002413"
     CDS             complement(544..804)
                     /locus_tag="ACG8PO_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0207699.1"
                     /translation="MGVDHLIVLSKLLFWIPFIIMTSAFFYLFKRPNKYLLLSACPMI
                     YFIHKIRGFYYYDSGISLFCYEIGLLASAIFFIFVLEYFRKK"
     gene            complement(941..1354)
                     /locus_tag="ACG8PO_002414"
     CDS             complement(941..1354)
                     /locus_tag="ACG8PO_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HFQ0207700.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1810..2295)
                     /locus_tag="ACG8PO_002415"
     CDS             complement(1810..2295)
                     /locus_tag="ACG8PO_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0207701.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(2434..2649)
                     /locus_tag="ACG8PO_002416"
     CDS             complement(2434..2649)
                     /locus_tag="ACG8PO_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HFQ0207702.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVIGICAIYCLKFFPMITRKVDERY"
     gene            2873..3673
                     /locus_tag="ACG8PO_002417"
     CDS             2873..3673
                     /locus_tag="ACG8PO_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFQ0207703.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            3692..5560
                     /locus_tag="ACG8PO_002418"
     CDS             3692..5560
                     /locus_tag="ACG8PO_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFQ0207704.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            5553..6044
                     /locus_tag="ACG8PO_002419"
     CDS             5553..6044
                     /locus_tag="ACG8PO_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HFQ0207705.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            6032..6586
                     /locus_tag="ACG8PO_002420"
     CDS             6032..6586
                     /locus_tag="ACG8PO_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HFQ0207706.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            6604..7599
                     /locus_tag="ACG8PO_002421"
     CDS             6604..7599
                     /locus_tag="ACG8PO_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HFQ0207707.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVSTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2708 a         1212 c         1443 g         2236 t
ORIGIN      
        1 tgttactatg aataacataa taagaaagta aagtggattg atttgagatc tttttatact
       61 tcaaaaaaga taacacccaa ctttcaaaaa tatgttttgg aagttgggac aaacgagtta
      121 atacatttta atacattatt ttttattcaa aaaaaatagc acaatggcaa agatcgtaag
      181 agagatcata agccccaatg aatgactaaa aagaaaagca gaatcacatg ccatatcatc
      241 ccatatttct tcaagaaaat aaataagcgg gaaaaaggga aggaatagta gaacatcgat
      301 ccagcaccat ttttttcttc ttttgaaata acataaaaca ataaacatga acgtgcaggg
      361 aagtaaaaac aaagctttac tcataactaa caaaatacca gctcctttta tttttttctc
      421 ccccacattc aaagagtaac aatggtatgt acttaataat agaaaaatgg atcaacttgt
      481 atgaataaca gctgaaaaga tctgcaccaa ataaaaaact tccagccaaa actgactgga
      541 agtttatttt tttctgaaat attctaacac aaatatgaaa aagatggccg aagctaataa
      601 gccgatttcg tagcaaaaca atgagatacc agagtcgtaa taataaaatc ctctaatctt
      661 atggataaaa tagatcatcg gacaagcaga taagagtaag tatttgttag gccttttgaa
      721 taagtaaaag aaagcactgg tcattatgat aaatggtatc caaaacaaaa gtttacttaa
      781 gacaatcaaa tgatcaactc ccatattttt taccaagaga gatcataaca tctttatcgt
      841 aaaaacaaaa tatttttttg ctcattttga aaatttaaac atactcgttc ttatccaaat
      901 atataaaaat cgcccctcat tcatcgagag gcgataagtt ctaatttaac tcaatttttt
      961 ttgtttcttt tggctgataa gctgttttag gtaataccaa tttcagcaca ccgtttttaa
     1021 aggttccagt gattttttct tcatcaacat taggaagata gaatgaacgt ttgtaagaga
     1081 ccgaatgtct ttctttccgt acatagttgc catcttcttt atcttctgta tgggactcat
     1141 gattggcgga gatcgtcaaa gtattatctg aatattcaac tgtcgtgtct tctttgtcac
     1201 agccaggcat atcagcagtt agtttgtatt ctttttcatt ttcgactaaa tctacttttg
     1261 gataacttga aacacctaaa aaatcattga atgctggact aaacacgtca ttgaaatcag
     1321 ggaacatgtc gcgaatgcta ggtacatttg ccatttttga tcatcctttc aataagtatt
     1381 ttttatttat ccctatttac gtaataactc tattttgaaa aaaatgcaag tataatggtt
     1441 agctttgtga atatttcatc taatacgata caaaggctat ctttctaatt tgaaaaaggg
     1501 atcattaaaa taataagaag aaaatatgtc aaaaggtaaa aaagtaaatt cttctatcca
     1561 aatatagaaa aattgaatct agaaaaccgc ccgacaataa atttcattaa ctcagttgac
     1621 cgaaaatgaa gtagatacca aaaatttatc aagagagcaa gtagagaagt gagtatattc
     1681 ataagagaaa tagctgtaac gaattattag gttaatgcaa atgcttattt atactccgta
     1741 taactttctc agaaaaaatg ataccaataa tgacttgcta gtactgtaaa tccgtataat
     1801 atcatctaat caaaatggtg ttccatttgc atctgtcatc gtaatcgatc cttcgggtga
     1861 cactcgataa aaaccaacgc ttccagatcc tccttgggaa cggatttgtt tgctggaagc
     1921 tgtgaacaaa taatctcgat caattttttg ataaaagcta ataacaatat cgtcactttg
     1981 taattcggaa cggtgggcaa taagaaatag aagtgctttg tcagagtttt ctaatggaag
     2041 agaaggtaca gtgctttctt gcatttcaga tgttgaagaa atgctaaacg actgaatact
     2101 tgattggcta tgttgcgaat ctacattacc agtagaagta ttagtaatgg aagtagtaga
     2161 agtactttta gttgaaccaa cctcagttgt tgaaatggct gctgaagatt ctttaacaga
     2221 attctttgtt ttatatccac aggcagaaaa aagtattaca gttcccaaca agcctgcgta
     2281 aatcccttta tgcatgatag ctcctcctaa aatagattat ctttattcag tatatcactt
     2341 aaacaaagaa aagcagatgg tttaaaatga aaaaaccttc ccaataaagg gaaggttcat
     2401 gaagataata acttttttaa taattatagc tttctaatat ctttcatcaa cttttctagt
     2461 aatcattggg aaaaatttca agcagtagat cgcacaaata cctatgacga tataaatgat
     2521 cttcgcaaaa atagttgttg aaccaccagc aatcatagct actaaatcaa gttcgaacaa
     2581 gcctaccaac agccagttca accctcccac aatcaataaa gttaatgcaa tagcatctaa
     2641 agttttcatt agaattctcc tttcttaaat gataaaacaa ttatatatca aaaagagaaa
     2701 cattgcttat ataacgctca ggaattttag caatctatgc aaaaagatga aaattttttt
     2761 tgctcttcca ttagtacaca ttttttagct gaagctgtaa atttcatgtc ttaatttttt
     2821 ttagtgagct tttatactgg atttgtaaga aaaaagaagg agaggatcac atatgtcgga
     2881 ttggttggat attgcaggaa aagtagtaat tgtaactgga ggttcctcag gaattggaag
     2941 aagtatcgtc gaaaatttgt tgaagcagaa tgcacaagta gcgaattttg atgtaactga
     3001 atgccgtata cagcatgaaa acctactatc tttaaaagtc gatgtgtctt ctaaaacgga
     3061 catagaagaa gggatatata aagtaatgaa acatttcaag acgatcgatg gtctagtcaa
     3121 taatgcagga ataaatattc cttctcttct aatagataga aatcatccaa aaagcaagta
     3181 tgaattatca gaacaagttt ttgataagat gatcgctgtc aatcaaaaaa gtgtatattt
     3241 gatgagccaa gcagttggaa gaatcttggt gcaaaaggga tcaggagtga tcgtgaatct
     3301 ttcttctgaa tcaggattgg aaggatcaga aggacagagc tgttatgctg ctacaaaagc
     3361 agcgatgaac agttttactc gttcttgggc aaaagagtta ggaaagagga atgttcgtgt
     3421 ggtaggtgtt gcaccaggga tactggaaga aaccggtttg agaacacaag aatatgaaga
     3481 agctctttct tatacgagag gtataagcgt cgaacagctg agaaacggtt acagtcgaac
     3541 aagtacgatc cctctaggaa ggagtggtaa gctgcaagaa gtggcggatc tagtctgcta
     3601 ttatctttca gaaagatcaa gttatatcac tggagtcacg actaatattt caggaggtaa
     3661 aacaagagga taaaggatag gggggacgca cgtgacgcta gtaaacagat ggtaccaatt
     3721 attgaaatta ctagtcactc acaagaaagt ttcaatccag gaagtgcaaa aagaaatcaa
     3781 aaccagtaat caaaccataa aaaaagatat tggggagttg aatgaagaaa ttgccggtat
     3841 tgcaaaaatc atccaaaaaa acaaacattt tgaacttgaa atttatgatt ttgatttttt
     3901 tgatgagatc atgcaaggaa aattgaaaac agccagtgat ttcaattcgg catccaaaag
     3961 aatcgcttat ttactaaata ggctgatcga aacagaggat ttcttacgta tggatgattt
     4021 gtctgaagaa gtcggtgtca gcagaggaac agtggtcaag gatctaaaga cattaaaaga
     4081 gatgatcaat gattttgatg taaagattac aggcacacca aataaaggac taagaataat
     4141 aggagatgag ctagaattac gactactttt attttatttt gtttctccct acttttcaga
     4201 gacgaaaaca gaacagtttc tagaagaaaa acaagcaatc aaacattttc agaagaagac
     4261 gaatgctagt aaacaagaaa tcaccctttt aacgaaagtt atcggtattt ctttgaatag
     4321 aatctctagt aactatctgt tgaagaagcc catcaatcgt tacagtgggt gttttgaata
     4381 tatccaagaa ttcaatgaac tgatcaatca gttggaagaa atctatgaac taactttgag
     4441 ccaatatgaa aaagatttta tcagttttcc tttgaacatc atcaacacaa gtataacgat
     4501 gataaggaaa gctaactctg atttgcgctt atatttcgac cccatgatga tgcgtattcg
     4561 acgattagat ctcattgatc tggatgagtc cttcttatat caagagatga aacagcacct
     4621 ctctttattg ataaatcggg taatttttcg ttatcgattg actgatttat tttatggtga
     4681 gatagaaaaa aagtatcctt tttcttacca aatcgctagc gaatgcatac aagcattaga
     4741 agctatcttg caacataaga tctctaaagt agagatcagc tacctaacgt tgtatttgga
     4801 gttagcattt cgatatcaaa aagagcattt ttcctctaaa aaagtagcca ttgttcatac
     4861 gacaggaaaa ggaacagcat taatgatcca aagacaaatc aaaaggatat tggggaggga
     4921 tatacaaata acacatgttt cagaagaaca atatgaaaaa gtagatctaa acaaatattt
     4981 cgctgttttt acaacgattc cgttaaaaaa tgtaaatgaa gagacaccgg tgatccattt
     5041 gactaatctt tttgatgact cttggttaag ggcacaatgg caaaaagcaa aaaaaaagcg
     5101 attagccggg tttcattcta ctgttttttt ctatcaaata tggaaagaaa aaaagacgta
     5161 tcaagaatat ttaaacaaat tagtcgctcc tttgatcaga ggctattatg tggatgaaga
     5221 atttgaaaaa aggatcttga aaagagaata tcagcaaagc acatttgtta acaaagaatt
     5281 tgcttttcct cacacaacca accagctatc tcaacacatt gttttatcag tagggctaat
     5341 aactgatcaa ggtttagcag aaacaggagg aaatggagtt atttttttat ttgccatacc
     5401 tgaagaaatg acggctgaag ttgagaatga attatttgag ctatacgata tcctatttga
     5461 actcataaaa atgggaatag atgagtttat tgaattagca ccgacacaag aatcattttt
     5521 aaaactaatc aaaagtaaag gagagagact tcatgaatga tatgtatcta ttcatagttt
     5581 tcgcagcttc agcttatgtg ctgcaagcag ttatcagttg gaagcaacta aagcatttca
     5641 atcaaacata tagcgaactg cgaagaaaag gaaaagtagc gattggtaaa cgacctggga
     5701 aaatccgttc aggcacgatt gttatgctag ctatagacga tcatgatcaa gttttagaag
     5761 tcagaaaaat gcaaggggtt acatctttgg caaaatttca ttcattacct gtctatgcgg
     5821 gtgaagatat ccattacatc gatcgttacc atccctcagt tcgagcggaa gatcgtttga
     5881 cacaagcagc tttagaaaat gccagagaag tatacttacg aatagaggca ggaaatgaaa
     5941 aaagtgaaca accttcattg ccttttattg ctatgctcaa taattttcgg atatggaaaa
     6001 gtacgatcaa agaaaaaagg agtgacaaac gatggatatc ataacaaaat ttgctgaggg
     6061 atttatgagg ctgttccaaa caggtgcaga aacattcata agctggatga caggaatcgt
     6121 accagttgtc ttgatgctga tggttgcaat gaatacgctg attgcttttt taggtgaaga
     6181 ccgagtaacg aaagtagcca aattatcagc gaaaaatccg cttactcgtt accttgtgtt
     6241 accgttctta tctgctttta tgttgggaaa tcctatgtca tttacaatgg ctcgtttcct
     6301 tccagaatac tacaaaccaa gctattatgc tgctcaagct caattttgtc atacgagcaa
     6361 tggtgtattc ccccatatta atcctggaga attgtttgta tggatgggga ttgctcaagg
     6421 gatcgaaacc ttagggctaa attcgatgga gctggctatc cgatacttgc ttgtgggact
     6481 gctgatgaac tttattggtg gttggataac tgattttact actggatttg tatgcagaca
     6541 gcaaggaatc gttcttagca aaaaagtaga gctttcagtt gattagagaa aggagagttt
     6601 taaatgtatg gtgatattaa gatcgtaaga ggaaacggcg ggtatggagg tcctttgatt
     6661 attaggccga caaaagataa acacaagttc atctatgtca caggtggagg tgagaggcca
     6721 atgatcgtag ataaaataga agaactgaca ggtatggagt ctatcaatgg gttcaaaaca
     6781 tcaatacctg atagtgaaat cgcgcttgca atcgttgact gtggagggac tcttagatgt
     6841 ggaatttatc ctaaaaaagg gataccaacc atcaatatcg tttccacagg aaaaagtggc
     6901 ccattagcac aatatatcac tgaagatatt tatgtttcag cagtagggtt agaacaaata
     6961 cagccactaa tggaaactga tgataacaaa gaaacagtaa atactgcatc tccagataaa
     7021 aagtacaaat atgatacttc taaaaagata accgagcaaa aagcagagca gcaaaactgg
     7081 atagcaaaaa ttggaatggg cgcgggaaaa gtagttgcta cattcaatca atcagcaaga
     7141 gaagcagtag atacgatgat ccggacagtg atccctttta tggctttcgt ttcccttttg
     7201 attggtgtga tacaaggttc tgggatcggt aatgtatttg ctaacattat ggcaccttta
     7261 gcaggaaatg cagttggatt aacaatcatt ggctttattt gttcactgcc atttttatct
     7321 cctttgcttg gtccaggggc agttattagc caagtgattg gaacattgat cggtgtagag
     7381 attgggaaag gaaatattcc tccccaatta gcattaccgg cactatttgc tatcaatact
     7441 caaaatgcat gtgattttat tccagtcggt ctaggtttgg aagaagcaaa agctgagaca
     7501 gtagaagtag gtgtaccatc agttctttat tcaagatttc ttaacggcat cccacgagtt
     7561 atcattgctt gggtagcaag ttttggatta tatagctga
//
LOCUS       DAZKKW010000105         7568 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.107, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000105 DAZKKW010000000
VERSION     DAZKKW010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7568
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.107"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(56..349)
                     /locus_tag="ACG8PO_002422"
     CDS             complement(56..349)
                     /locus_tag="ACG8PO_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HesB/YadR/YfhF family protein"
                     /protein_id="HFQ0207708.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEIDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(377..1042)
                     /locus_tag="ACG8PO_002423"
     CDS             complement(377..1042)
                     /locus_tag="ACG8PO_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HFQ0207709.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(1182..1814)
                     /locus_tag="ACG8PO_002424"
     CDS             complement(1182..1814)
                     /locus_tag="ACG8PO_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFQ0207710.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(1821..2888)
                     /gene="liaS"
                     /locus_tag="ACG8PO_002425"
     CDS             complement(1821..2888)
                     /gene="liaS"
                     /locus_tag="ACG8PO_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     LiaS"
                     /protein_id="HFQ0207711.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            complement(2885..3616)
                     /gene="liaF"
                     /locus_tag="ACG8PO_002426"
     CDS             complement(2885..3616)
                     /gene="liaF"
                     /locus_tag="ACG8PO_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein
                     LiaF"
                     /protein_id="HFQ0207712.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            complement(3785..4264)
                     /gene="greA"
                     /locus_tag="ACG8PO_002427"
     CDS             complement(3785..4264)
                     /gene="greA"
                     /locus_tag="ACG8PO_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFQ0207713.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     KEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(4400..5575)
                     /gene="mltG"
                     /locus_tag="ACG8PO_002428"
     CDS             complement(4400..5575)
                     /gene="mltG"
                     /locus_tag="ACG8PO_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HFQ0207714.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
     gene            5779..6948
                     /locus_tag="ACG8PO_002429"
     CDS             5779..6948
                     /locus_tag="ACG8PO_002429"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFQ0207715.1"
                     /translation="MKKGKHAKKRKYSFLMIVVVLFSLSAFSTGTILGWQFYNNEHSQ
                     IVAHAQSAQKKLILTTNEQQLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKL
                     TQQPNDPSLYYQIGSVQKALTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDL
                     LYMRSGLHRTASPTIPMTDEEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQ
                     PYEKLLTDDIIEPLGLQHTKFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNEL
                     GTGNISMSVYDLNTLFTQVLSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQG
                     NIDSFDTSAIFSKDSQDAIIMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
     gene            complement(7215..>7568)
                     /locus_tag="ACG8PO_002430"
     CDS             complement(7215..>7568)
                     /locus_tag="ACG8PO_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0207716.1"
                     /translation="QLLLKNHQVTEDGQVIYSSFSPNKDEITFTLKSSKGKTIVLPVF
                     RYKGLTVTVNSKNATVLKQGGPFVTVKSESGINKVTIRYSYTFFSKISLFISIITLIV
                     LLFYCIKLKIRTSHE"
BASE COUNT         2294 a         1518 c         1159 g         2597 t
ORIGIN      
        1 atattttcaa aagaaaaaag agaaggtgac aaaaattgtg tcaccctctc tttttttatt
       61 gttttttaaa gtgataggtt ggctcctcca ggttgggatc ataatcaaca agtaaatcat
      121 atcctttaaa gaaccattca tctgcttcct cgataaaaaa aagtgtccca tcaatttcca
      181 ctttctttac aggatgttct ggctgttcaa ctgacattcc aatcgaaaaa ccatcatgaa
      241 cctctgtttt gccgtagact tttccaaaaa aacgtattcc gtaagtttct ggcagatcaa
      301 tttctgagtc aaaccacttt tgtgcttttg gcgttaccgt taatttcatc ccaacaacct
      361 actttctctt ttctttctat tgattcatct tatcgtctag aatatccaca tcgcctgtct
      421 ctccaataac cagtagatta tcattttcac gaacatattc atctgctgca ggagaagcga
      481 taaccgtttg atctactctt cgtatggcga ctactgttaa gtcaaaacgt tgcctgaaat
      541 ttaattcagc taatgttttg ttgaaaaatt taggattgga gactcggatt tctgctaaag
      601 aatatttatc tgacaactct aggtaatcta aaatattctt tgacactaaa ttatgcgcaa
      661 tccttattcc catgtctcgc tcgggatgga cgacgcgatc agctccgatc ttttcaagta
      721 ctctggcatg atactcattt tgggcttttg ctaaaatatt tggtacgccc atttccttta
      781 ccatcagagt aactaagata ctggcttgaa tatcttctcc aatagcgaca attacatgat
      841 cgaaatttct tatgcctaat gatcgaagtg tcatttcatc ctgtgcattt gccacaacag
      901 catgagtggc tatattcata tattcattga cccgatcttc attactatca atagccagta
      961 cttcttgacc agcttcgatc agtgttcggc agatacttcc gccaaatcgt ccaagaccta
     1021 caattgcaaa attttgtttc accgttttcc tcctttagta tcttcttttc tcagtataac
     1081 aaagccttta caaaaacaaa agcaagcatc attattgtaa aaaagccaag aaaaataata
     1141 gttattttct cagcttttga ttatcctctt tatcatcgtt tttatttcac caaaccatgt
     1201 ttgaatgcat agatagcggc ttgtgtacgg tcttccactt ctagcttagc caatatattg
     1261 gaaacatgtg tttttactgt tttcagtgta ataaacaatt cgtccgctat ttcctgatta
     1321 ctttttcctt cagaaatcag cattaaaatt tcattttccc gattcgttag ttcttcatgt
     1381 aatacaggct catttcgccg actcattttt tccatcattt ttgttgtcac ttctggttct
     1441 aatactcgct cgccacgttg cgttgccctt atagcatcag caatttcatg tgcggtagag
     1501 gtcttcaaca aataccctgc tgcacctgct tcaatggcag gataaacttt ttcgtcatca
     1561 ataaaactgg ttacgataat gattttagct tctggccaat cttttaagat tgcttttgtt
     1621 gattcgatac catccatttc ttccatgact aagtccatta aaatgacgtc cggtcgaagt
     1681 gtcatcgcct tctcatagcc ttgtcgtcca ttttcagctt ctccaattac ttcaatgtct
     1741 tcttgaatag ataaataaga agagacgcct aagcgcacca tttcatggtc atctactaat
     1801 aaaactttta tcatccttat ctatgcctcc ttcatcaaag ggaccttgat ttctacactt
     1861 gtaccttgac ctttaaaact aatgattttt actgttccac caataccagc tactcgttct
     1921 ttgatattgt tcaatccata acttcccgtt ttcgtttcat tcatattaaa ccctgttccg
     1981 tcgtcaataa tacgtaacaa aagattattg tctattttgt gcaaatatac ctctaattca
     2041 ttggctttag catgtcttaa tgtatttgat aacaattctt gaacgattcg gaacaagtga
     2101 tcctcaatgg aactagatag ttttacatct tctacatccc atttcagcga aatctggatt
     2161 ttattttgca attccttcaa taactgctcg ataccttgct ttaaactttt ctcttctaaa
     2221 ttgactggac gtagatgaag aagcagcgca cgcatctcag actgggaagc gttgatgatc
     2281 tctgctacca ttttcaactg cttgcgaaac atttctggtg tctcgctttt ttctgcttgc
     2341 tccgtcaatg ccgacatcat catcattgct gcaaacaatt gttgcgaaac tgaatcatgc
     2401 agctcacggg ctaatcgatg tctttctaat tctaaaattt cttctttggt ttgtccatct
     2461 acatattggg gacggctcgt tacaatttgt aattcactag atacttcaat cattttttct
     2521 tttatcttag tgatttcctt atcaatatct ttgatgtata aatcgtccga tgcacttggg
     2581 atcggaagaa gcaacaattt ggattcataa ttgccagaag aaagtgcacg cagcttttct
     2641 tcgattttcc cgtattttgt cttttgaata agtgataata atagaaagac aattaatcct
     2701 acacctaagg aaatggctaa caaatggaag ataagcggaa catataataa acgtgcacgc
     2761 agtaattctc cccaccaatg actttgattg ctggcataaa aataagtgaa aagtgaaaat
     2821 agaataataa ggaaagccgc aatccccgaa taaacagcta gcatcgctct ggatattttt
     2881 cccatcatat tcgtatcacc tcgacatctc caatcaaggt attagtaata atcttcaatc
     2941 tacgagggtt ttcatcataa tcactgctgt agattttgat ttgttcattt ttcaaagaaa
     3001 attgctcttc ttcaaacaag acatttccta ctaatgtcgc atgctctaat cggatggcta
     3061 cgcccaaagg tactaagatc cgtgttctgc ctatcccttt tcttacaatc acaatattat
     3121 catcttttgg caaaagcgta ttgcccaaat ctatgatagt atcaccagaa atgacggcga
     3181 tgttgatatc gtcccattca tagacgtcgt ttccaatacg ttggttaccg aataattgct
     3241 gtcttttccg ctcattatta tgtgttttta attgctctgt ttggatcatc atgatctgtt
     3301 tctttcgcca aaaagcattt ttggttaaat ctatccctga aatctctaca cctttcagac
     3361 caataaataa aacggcaaat acagccatca tccataaagc aggactgttg actaagctaa
     3421 aaaaaatgac caaacttcct aatatcaatt gaaaattttg aaatttagta cgaggatgtt
     3481 tgcgcatagc tagatatata ttgaaaatac cgaatatcac taaaagcaaa agtccagtat
     3541 tgtttacgat ttgccaaaca gcaaataaca gcagtagtgc ttcaactacc acaaaaaaac
     3601 gccaagaact attcatcgct ctttctccct cctgacacat tctcatttca tcatacctat
     3661 ttttttaact gttggctata ggactttaga tgtaagatcg tttgttgaaa tctaaaatac
     3721 acaaagagaa aaagatctag agtgtatcat cagtaagatg aactctctag atctttcagt
     3781 cagactacgc ttgttctact ttgacgattt taactaacat atcgccacct ggagtagcaa
     3841 tagttacttc atcattttta cgtttgccaa tcaatgcttg cgcaatagga gaatcattgg
     3901 aaattttgcc agaaaacgga tctgcttcag cgcttccgac aatcgtgtac tcttcttttt
     3961 ctccatctgg tagttcgatg aatgtaactg ttttaccaat tgatacctca tctgcatcta
     4021 caccgtcgtt atcgatgatt tgagcaaaac gaatcatatt ttcaattgtt gtgattcttc
     4081 cttcaacaaa tgcttgttca tctttcgctg attcatattc agagttttcg gaaagatctc
     4141 cgaaaccgcg ggcaattttg attcgttcaa tgatttctcc gcgtttgact gtttttagtt
     4201 cttctaattc tttttctaat ttttctttac cttctagagt cattggataa actttctcta
     4261 ccatgtgtaa cgtctccttt tttcgataaa ataccgcaaa aggtaatctt ttgaggaata
     4321 tagtccaaaa aagattacca cgcttttctt caaatgtaaa tgtttttcta aaaatgatca
     4381 atgtctagag cctgtttttc taagaattat ttacatattt ttccactaat tccatatgtt
     4441 cttcataagt tttagcgaaa taaacatttc cagtgctcgt atctgctacg aaataataat
     4501 agtcattttc tgctggttct aatactgcct tgattgcctc ttcgcttgga ttgtcaaaag
     4561 gtccaggtcc atagccggta ttggtataca aattatatgg tgaatctaca gctgtatctt
     4621 catacgtcac taattctttg tgttcaccta atgcgtataa aacagagata tctgactgaa
     4681 gtggcatatt tgctttgatt cggttaaaga agacttgtgc aatgttttta cgatcatttt
     4741 cttttacgcc ttctttttca actagagaag ctagagttaa aacttcttgt acagtcaaat
     4801 ttttctgctt gatagctgaa taatagtttt gcatgaccgt attcgtcttg ttcaccattt
     4861 caatcactag atccttaagc gaagtactcg tgtaataatc atatgtggct gggaataagt
     4921 aaccttccaa acggtatttt gtgttctcag ctttagatgc actttccaac agttctggga
     4981 atttttggtg caactcgtta aagaaagtct catcattcac taaatctaaa aattctttct
     5041 tgtctttacc agttaccttc gctacccgtt cagcaatctg atcgatatcg tacccttctg
     5101 gaatggcaat tttcccatct gcaacttttg ttggctcaga agtaccgcct tcttgcaact
     5161 gtttgccaat ctcgtcaaga gtcatattag gcgctaactg ataatagcca gcttgaaagc
     5221 cagttaaatt tttaaatttt gtataataat tgaaaacaat cccgctttta ataatattgt
     5281 ccttctccaa aatctcacca atctgcttgt tagaagagcc actcgggatc tcgacttgga
     5341 ctaattggtc atctgattta tctaaaggct tcaacccgct atctacataa cgatacacgg
     5401 taaatccaag aaatccgcca ataatcaata atgtaagagc aatcactaaa atgatcttcc
     5461 tcacaatctt atcttctttt ttacgcagat ttctccgctt gtcgattata tctgctgtag
     5521 ctgccttact ggcttctttt ttatctttac ttgggtgttg tttgtttcct gacaaaacaa
     5581 ctcctcctta ctatcaagtt cgtaacttta ttcattatac atgatatttg aattggcgtg
     5641 aatgatttta tttaaatatt ctatcaaaaa tgtaagtagt aaatccatta ctataagatt
     5701 aaaataaatg ttatcaagat acaagctatt ttagctttat tctttctgta aaaaagaaag
     5761 gagactaaat aaactattat gaaaaaagga aaacatgcta aaaaaagaaa atattcattt
     5821 ttaatgatcg ttgtcgtgtt gtttagcctt agtgcgttct ctacaggaac tattcttgga
     5881 tggcaatttt acaataatga acactctcag atagttgccc atgctcaatc agctcaaaaa
     5941 aagttgatac tgactaccaa tgaacaacag ctcgttgaaa aaatcgatca actattaaga
     6001 aaaaatgatt atattggctc aatttacgta aagaaaaata atcgcatcat catggaaaaa
     6061 ggctatggct atgcgaataa actcacgcaa caaccaaatg atccttctct ttattatcag
     6121 attggttcgg ttcaaaaagc attgactact cttttgattt taaaacaagt cgagcgtggg
     6181 cacctttctt tagatactaa gctggctcaa ttctatccac aaattgctgg cagccagcaa
     6241 attactatcc gtgacttgct gtatatgcgc tcaggcttgc accgaactgc ttctccaacc
     6301 ataccgatga ctgatgaaga agtcgttcaa tttgcgattc agcacttaaa atttacagat
     6361 tatcagacct ctcattatga ttcattgaac tttaccatat tgactggaat tttgatcaaa
     6421 ttaacaaatc aaccgtatga aaaattattg accgatgata tcatcgaacc tttaggatta
     6481 cagcatacaa aattttatga ccaagtcaaa aattcatcca atcatgcact atcctatcaa
     6541 atgtctaaag aggatgatta ctttaaacca ctgacagaat ctgaaacaga gatccataac
     6601 gaattaggga ctggtaatat cagtatgtcc gtctatgatt taaatacgct gtttacacaa
     6661 gttttatctg gaaaactagt ttccaaagac ttactcttct cattatggcg tgaaaatgcg
     6721 aatggacatc catatagtgg tggggtatat agcggagata attatatcct gtcgcaagga
     6781 aatatcgact cttttgatac atctgctata tttagcaaag attcacagga tgctatcatc
     6841 atggaaagca atataaaagc tgacaaaact atcaaactgc ctactgctga tctaagaaac
     6901 caaatctatg acctgatgga aggaactact aagttggcaa atagctgata tgatgaaaag
     6961 atagcaaata ctacttcttt gaatagcatt tgcttaaaaa aagtactcga ctaaacgtag
     7021 gaaagagtgt gggacaaatc taaaaatcaa tttttgctac agactttaat tccaaataaa
     7081 cagaacagaa agaggtgttc ttctttctgt tctgtccaaa ttattctaag atttctcttt
     7141 accaacatta tttgacaaag ggcctattaa atctaaaata gtatctttag attgctaaaa
     7201 tatgtgctag caaatcattc atggcttgtt cttattttta attttatgca ataaaaaaga
     7261 agaacaatta gcgtgattat agaaataaat aatgagattt tagaaaaaaa tgtgtatgaa
     7321 tacctgattg ttactttatt tattccactt tcacttttta ctgtaacaaa aggacctcct
     7381 tgttttagta ctgtagcatt ttttgaatta acagtaaccg ttaatccttt ataccggaat
     7441 acaggtaaaa ctattgtttt gccttttgaa gatttaagcg taaatgtaat ttcatcttta
     7501 tttggagaaa agctactata aataacttga ccatcttctg taacttgatg gtttttcaaa
     7561 agcaattg
//
LOCUS       DAZKKW010000106         7545 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.108, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000106 DAZKKW010000000
VERSION     DAZKKW010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7545
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.108"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(151..1449)
                     /gene="eno"
                     /locus_tag="ACG8PO_002431"
     CDS             complement(151..1449)
                     /gene="eno"
                     /locus_tag="ACG8PO_002431"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFQ0207717.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
     gene            complement(1565..2320)
                     /gene="tpiA"
                     /locus_tag="ACG8PO_002432"
     CDS             complement(1565..2320)
                     /gene="tpiA"
                     /locus_tag="ACG8PO_002432"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFQ0207718.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            complement(2424..3614)
                     /gene="pgk"
                     /locus_tag="ACG8PO_002433"
     CDS             complement(2424..3614)
                     /gene="pgk"
                     /locus_tag="ACG8PO_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFQ0207719.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            complement(3724..4725)
                     /gene="gap"
                     /locus_tag="ACG8PO_002434"
     CDS             complement(3724..4725)
                     /gene="gap"
                     /locus_tag="ACG8PO_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFQ0207720.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            complement(4766..5857)
                     /locus_tag="ACG8PO_002435"
     CDS             complement(4766..5857)
                     /locus_tag="ACG8PO_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="central glycolytic genes regulator"
                     /protein_id="HFQ0207721.1"
                     /translation="MGRFKSTLDVKCPTKEIAMLDEIKMIESVAPDIIEVLQERYKIL
                     RNIYWMQPIGRRSLSESMGITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSL
                     ENFMDQLLGTHQTEQQLAEYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEG
                     ENIIAVMGGTTMAVVAEQLSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKAN
                     GNHRALYVPEQLSLATYNSLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEK
                     ELVMLKQANAVAESFGYFFNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAI
                     RAYMKNAPKQTWLITDEAAANEILKGVTL"
     gene            complement(6006..7340)
                     /gene="rpoN"
                     /locus_tag="ACG8PO_002436"
     CDS             complement(6006..7340)
                     /gene="rpoN"
                     /locus_tag="ACG8PO_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HFQ0207722.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            7400..7471
                     /locus_tag="ACG8PO_002437"
     tRNA            7400..7471
                     /locus_tag="ACG8PO_002437"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7433..7435,aa:Arg,seq:ccg)
BASE COUNT         1959 a         1594 c         1392 g         2600 t
ORIGIN      
        1 cattctttgt aaacatcata tacagaagct agttgcaaaa aaactctcta cctgctgata
       61 tttcagcaag tagagagttc gattacttaa ctaagtgatc atactcgact atctttcaat
      121 agtcgatcat gtatcttcat tagaaaagaa ttatttgttt tttaagttgt agaaagattt
      181 caaacctttg tattctgcaa cttcaccaag ttggtcttcg atacgtagta attggttgta
      241 tttagcaata cggtcagtac gtgatagaga accagttttg atttggccag cgtttgttgc
      301 aacagcgata tcagagattg ttgaatcttc tgtttcacca gaacggtgag atacaactgc
      361 tgtgtagcca gcttctttag ccatttcgat agcttcgaaa gtttctgtta atgtaccgat
      421 ttggttcact ttgataagga ttgagttagc aattcctttt tcgatacctt cagataattt
      481 ttgagtgttt gttacgaaca agtcgtcacc aactaattga actttgtcgc ctaatacgtc
      541 agttaatttc ttgaatccgt cccagtcgtt ttcgtctaat ccatcttcga ttgagatgat
      601 tggatattta gaaactaatt cttcgtagaa tttgatcatt tcgtcagttg ttttttcgcc
      661 ttcgcctgag tcagctagta cgtaaacacc tttttctttg tcgtagaatt ctgaagaagc
      721 agcatccata gcaagaacaa cgtctttacc aggtacatag ccagcttttt cgattgcttc
      781 gatgattact tcaaaacctt cttcgtttga accaaggttt ggagcaaatc caccttcgtc
      841 accaacagaa gttgctaatc cgcgagcttt caagattgaa gctaatgcgt ggaatacttc
      901 agcacccata cgtagagctt ctttgaatgt tggagcgcca acaggcatga tcatgaattc
      961 ttggaagtcg attgagttat cagcatgtga tccgccgttg atgatgttca tcattggtgt
     1021 tggcaatact tttgtgttga atccgcctag gtagtggtat aaaggtactt ctaggtagtc
     1081 agcagctgca cgtgcaacag cgatagaaac acctaaaata gcgttcgcac ctaatttacc
     1141 tttgttagga gttccgtcta aagcgatcat agctttgtcg atagccattt ggtcacgaac
     1201 atcgtagcca atgattgctt cagcaatgat gttgtttacg ttgtcgacag ctttagttac
     1261 acctttaccg ccataacgag ctttgtctcc gtcacgtaat tcaactgctt cgtattcacc
     1321 agttgaagca cctgaaggaa ccattccgcg gccaaaagct ccgctttctg tgtatacttc
     1381 tacttcgatt gttgggttac cacgtgagtc taagatctcg cgagcgtaaa catcagtaat
     1441 aattgacatg ttttgtctct cctttgagtt tgttttttcc taagggattt ccctcatata
     1501 cgattttagt taattttgct cattagtgca aacatttctt gcatttttcg caaattttca
     1561 acaattattt tacagcgtct aacaatgaca agaatgaatc tgcttgcaag cttgcgccac
     1621 cgactaaagc accgtcaaca ttttcttttg ccatgtattc agcaatattt tctggtttta
     1681 cagaaccgcc gtattggata cgaactgctt ctgatacttc ttttccataa agtttttcaa
     1741 ctgtttgacg tacaactcca caaatttcgt cagcgatttg tgcgtcagct gatttaccag
     1801 taccgattgc ccagattggt tcataagcaa taaccatgtt tgacacctgt tcagctgaaa
     1861 gatcagctaa accagctgta atttgtcctt cgatccattc agcagttttg cctgcttcat
     1921 aggtttctaa agattcgccg cagcatagga ttggtgtcat accatttgca aagatagctt
     1981 tcgctttttt attgatgtct tcgtctgttt catggaaata ttcacgacgt tctgaatgtc
     2041 cgatgatcac ataatgaaca cctagatcag caagagctgc tggtgaagtt tcaccagtga
     2101 acgcacctga gttttcccag tagcagtttt gtgctgagat ttttagttct gtaccttcag
     2161 ctgatgtcat taattcttgt aagaataatg caggagatcc gatgactgaa tcaactttgt
     2221 catttgaagg gattttgttt tttactgctt cagcaaattc ttttgcttct gatgcagttt
     2281 tgttcatttt ccagttacct gcgataatag gtttacgcat tgaggaacac actccttaat
     2341 ttattgaaaa atggctaggg agtcatgaaa actcccttca ccttttgttc gattactcgt
     2401 cagaattgag caaacttggt cgtttattta tcgttgattg ctgctagtcc tggtaattct
     2461 ttaccttcaa gcaattccaa gcttgcgccg ccacctgttg agatgtgtgt gaatttgtca
     2521 gcaaatccta attgttctgc tgctgcggct gagtctccgc caccgatgat tgttgttgcg
     2581 ccttctaagt tagcgattgc ttcacaaaca ccgattgtac ctttagcaaa gttactcatt
     2641 tcaaatacac ccataggtcc gttccaaaca actgtttttg caccttgtaa tgtatcagca
     2701 aataatttta ctgatgctgg gccgatgtct aaagccatca aaccatcagg aatgtctcct
     2761 tcgattactt gtgtttcaac atcatttgaa aattctggtg cacagacatt gtcgattggc
     2821 aagacaagtt tgtcgcctgc tttttcgatt aattcttttg ctagttccac tttatcagct
     2881 tctactagag agttaccgat cttgattcct tttgcttcgt agaatgtgta agtcattccg
     2941 ccaccgatca agattttatc tgctttagga atcaagtttt cgattacgcc gattttatca
     3001 gaaacttttg cgccaccaag gattgcaatc attggtcgtt ttggttcttc tactgcttca
     3061 ccgatgaact tgatttcttt ttccatcaag aatccagcaa ctgttgggat accagttgaa
     3121 gcgataccta cgttagaagc atgtgcacgg tgagctgtac cgaaagcatc gttaacaaat
     3181 acatcgccta atgaagccca gtatttacct agttctgtat catttttgct ttctttttta
     3241 ccgtcaatat cttcaaatcg tgtattttcg aatacgacaa cgtcgccgtc attcatgttg
     3301 cggattgctt cttctaattg ttctccgcga gtttcaggta cgaatgttac ttctttacct
     3361 aataattcgc ctagacgttt tgcaactgga gctaaagatt ttccttcttt atcttcttct
     3421 gtttttacac gtccaaggtg agagaaaagg attgcttttc cgccttgttc tagaacgtag
     3481 ttgattgttg gcaatgctgc tttgatacga gtgtcgtcag tgatcacgcc gtctttcaaa
     3541 ggaacgttga agtcaacacg gacaagaact tttttgtctt ttaaatcgat atctttcact
     3601 gttttcttag ccattttatt tcctcctgaa taaacttatt atcaaaaaaa gcggaagaag
     3661 cgcgattgct tccccgcttt taaatgtaca tgtctataat ttacacttgt acgtttgtct
     3721 atcttataag ttagcgaagt attctaaagt acgtactaat tgtgcagtgt atgacatttc
     3781 gttgtcgtac caagcaacag ttttaactaa ttgtttgtcg ccaactgtca ttactttagt
     3841 ttgtgtagca tcaaataatg aaccgtaagt cataccaacg atatctgaag aaacgatttc
     3901 gtctgtgttg tatccgtaag attcgtttga agcttctttc attgctgcat tgatttcgtc
     3961 aacagttact tctttttcaa gaactgttac taattctgtt aatgaacctg ttggtacagg
     4021 aacacgttga gcagctccgt ctaatttacc gttcaattcg ggaattacta aaccgatagc
     4081 tttagcagca cctgttgagt taggaacgat gtttgcagca gcagcgcgtg cacggcggaa
     4141 gtcaccttta ggatgaggtc cgtctagagt catttggtca cctgtgtaag cgtggatagt
     4201 tgtcattaat ccttcaacaa caccaaattt gtcgttcaat gttttagcca taggagctaa
     4261 acagttagta gtacatgaag cacctgagat aactgtttct ttaccagtta atgtttcgtg
     4321 gtttgtgtta taaacgattg ttggaacatc gtttccgcca ggagcagaga taacaacgcg
     4381 tttagcacca gctgttaaat gtttttcagc tgctgattta gaagtgaaga aaccagtaca
     4441 ttctagaacg atatctacgc ctagttcgcc ccatggtaat tcttctgggt tgcggttagc
     4501 taatacttta acttcttttc cgtttacgtt gaatgaacct tcatgaactt caactgttcc
     4561 gttgaaacgt ccttgagttg tgtcgtattt taacaagtgt gccaacattt tagcatctgt
     4621 caagtcgttg attgctacta cttcgattcc atctacatct tggatacgac ggaatgctag
     4681 acgtccaata cgtccaaaac cattaatacc tactttaact gtcatttaag atttcctcct
     4741 tatgaaaatc aaaaaaatat ttattttaaa gggttacccc ttttaaaatc tcatttgcag
     4801 cagcttcatc agtgatgagc cacgtttgtt ttggtgcatt tttcatataa gctcgaattg
     4861 ctttagcttt tgattttccg cccgcgattg ccatgatgat cgggactttt tccaaatctt
     4921 gcaattctat gccgattcta gggaccttat aaacgacttc acctttttca ttgaaaaagt
     4981 agccaaaaga ttccgcaaca gcatttgctt gtttcagcat gactaattct ttttcagtca
     5041 tcttacgacg tgctgccatg tgtaatgcac gcccaatact atgaataaca caattcgctt
     5101 cactaatcaa gtttaagact tcttggatag atggctcatt gagtaaagaa ttgtaagttg
     5161 ccagacttaa ttgttccgga acataaagtg cgcggtggtt accgtttgct ttagcagcca
     5221 ttcttgcact gacagaattt gcctgtacag ttatcgcttc accaatcccg cctcttgcag
     5281 ggacaaacag gttatgccgt ttcttatttt ccagattaga caattgttcg gctaccacag
     5341 ccatagtagt tcctcccatc acagcgatga tattctcgcc ttctggaagt agtcggtcta
     5401 aggcttcgct cagcgcttcg ccgaatgcat cagccacttt ggtctgctct tcgctgtttc
     5461 ctgaaacaac gatacagcgt tggttaccaa aatattctgc caactgttgt tcggtttgat
     5521 gcgtacctaa aagctgatcc atgaagtttt caaggctttg ataaacttct tcgcccttag
     5581 ttgtcagcgt cataccgctt tttgaggtgt cgatcaaatt cagatttttc aaaaggtctg
     5641 tttctgttcg taagactcgt tctgtgatcc ccatactttc cgataaactt cgacgcccaa
     5701 tcggctgcat ccaatagata tttcgtaaaa ttttatagcg ttcttgcaac acttcgataa
     5761 tatctggtgc aacagattca atcattttta tttcatccag catagcgatc tccttagttg
     5821 gacactttac gtccaatgta gacttaaaac gacccatata cgataaaaaa agaaattaac
     5881 tattcgaatt tcacatcaat aaagggaaat ctcaacctca cgactgtaag tctaacagtt
     5941 ttgctctcgt ttttcaacta aaaagactag aaaaagtgaa atactttttc tagtcttttg
     6001 actgattagt tatcataacg ttttcttttt gatgaactcg ggattcctaa taaatcacga
     6061 tattttgcta ctgttcgtct tgagatcgct atctcatctt ttttcagcaa ttcaaccaat
     6121 ttctggtcag acaacggctt gtttttgtct tcttgatcga ctaattcctg cagctttttc
     6181 ttcgctgtat ctgctgaaag gtcttctgtc tgttctgtac tattcgtagg aattcgtgta
     6241 gtgaaaaatt tttttaattc aaatacaccg aagtctgttt ccaaatattt cccgttgact
     6301 gcacgactga ccgtggattc atgcacatct atctcagttg caacttcctt taacgtaaga
     6361 ggcttgattg gacgttcctt gtcgataaag aaagcttttt gccgggaaac aatgacttga
     6421 gcaacccgca aaattgtatc cccacgttgt aggatcgttt tcttcaacca ttcgaattcc
     6481 tgtttccgtt cttttaaata attttcagtt tctttatctg cttgttgtga catttgtttg
     6541 aagtaactct cttgaaagcg tatatttggc tgattttttc ggtttgagat aacttgtaca
     6601 tttttatcgt cgtccactaa tacccggaca tcaggaataa tgtacaattc gctgctacgg
     6661 tcgaaaatcc ggccagggct tggactcaac gtttgtaaat agtcgaatat ctgctgaaca
     6721 gcatgtagat caacagaaaa acgttgtgcg atttctttcc attttcgctc caccagttcc
     6781 tcaaaacatt cttccaacac gatataagct aattccggcg cataatcatc tcgttctgtt
     6841 tgaagcatga gacattcttg caaactacgt gcacctactc cagctggttc taattgctga
     6901 atcagagtca acgcatcgag catttggata ggcgtagctc caatctcttt tccggcttct
     6961 tctaaactaa tcgtcaaaaa tccgttcaaa tcgatgtatt ctacaagata aagaacgatt
     7021 ttcctcaaaa aagtatctcg gtaattcaaa tggacttgtt cgatcaatga atcaaataaa
     7081 gacccttttg tatcaggaat ctgatttagg taatttgtct cttctccttc ataagaagat
     7141 gaagaagctt ttatgtaatc tggctgccat tccggctcaa caacttccac caatggattt
     7201 tccattgctt gattctcaac gaatgccaac agctcctcac tgttatattg taaaacttgg
     7261 atggactgtt gcaactgttg cgtcatggct agtttctgtg tctgctgctg tttttgtgaa
     7321 aaattttgct caaatttcat ttttttgtcc aatccccttg taatttttta tctgtttagt
     7381 tacaattatc ccagtgcttg cgtccttagt gtagtggata tcacgtaaga ttccggttct
     7441 tgagacgggg gttcgattcc ctcaggacgc gttctactgc tattttagca taatttacga
     7501 atagttgctt ctttattttt aagtgaaaac tgtccgccaa agaaa
//
LOCUS       DAZKKW010000107         7496 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.109, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000107 DAZKKW010000000
VERSION     DAZKKW010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7496
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.109"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            299..772
                     /locus_tag="ACG8PO_002438"
     CDS             299..772
                     /locus_tag="ACG8PO_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HFQ0207723.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            769..2901
                     /gene="feoB"
                     /locus_tag="ACG8PO_002439"
     CDS             769..2901
                     /gene="feoB"
                     /locus_tag="ACG8PO_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HFQ0207724.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            2903..3037
                     /locus_tag="ACG8PO_002440"
     CDS             2903..3037
                     /locus_tag="ACG8PO_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HFQ0207725.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            3131..3775
                     /locus_tag="ACG8PO_002441"
     CDS             3131..3775
                     /locus_tag="ACG8PO_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0207726.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            3962..5155
                     /locus_tag="ACG8PO_002442"
     CDS             3962..5155
                     /locus_tag="ACG8PO_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HFQ0207727.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            5148..6875
                     /locus_tag="ACG8PO_002443"
     CDS             5148..6875
                     /locus_tag="ACG8PO_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HFQ0207728.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            7269..7466
                     /locus_tag="ACG8PO_002444"
     CDS             7269..7466
                     /locus_tag="ACG8PO_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HFQ0207729.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
BASE COUNT         2446 a         1280 c         1659 g         2111 t
ORIGIN      
        1 ctgtggaata ctaagttttg tgtttttgaa aaaagagctg aaaacaagta tcgtgtggga
       61 aaatagcaaa tttaatagag aaaaaattgt ggaaaactat gaagaaatat ttttttctca
      121 tttttatcta tcaatactaa agattcgctc ataatatcac aagtataatt gacattctca
      181 attaccgaga gtattctatg tgagaatgat tctcatttat ttgcgtaaca cgtaagaaaa
      241 ttagaacata taatgaatga ttttaataca tgatttattt cacaagagag gaatcaatat
      301 gcagacctta gcgcaggcaa agcagaattt cgtttatcaa gtcgaacgaa tcgaaacaga
      361 agaaaaaaca aaacgccatt tgcaaaatat cgggatcgtt gccggaagta aaatcgtcgt
      421 cgtcaattta tcaggcaaca atgccatttt actggtaaaa agtcatcgag tggcagtaag
      481 tcgggaaata ctaggtcaaa tctttgtcaa taaaaagact gcttcctctg aaagctggac
      541 ttctcttgat caattaaaag caggagagac tgggatcgtc gtagcaatcc acggacaagg
      601 cgcagtgaag cgacggttaa tggatatggg attgacgcga aatgtaaaaa tagttatccg
      661 aaaactagcc cctctgggag atccaatcga actgacagtg cgagggtatg aactgacgtt
      721 acgaaagtca gaggccgaac taatcttgat acaaagggag gaaaaagaat gaatatcgcg
      781 ttagtcggaa atccaaacag cgggaaaact agcttgttca atctcttgac tggttcgaat
      841 caatatgtag ggaattggcc gggcgtaaca gtagaaaaaa aagaaggatc gttgaaaaaa
      901 aatccgaaca ttcatattca ggacctaccc ggcatctatt ctttgtctcc ttatacacca
      961 gaagaagtga ttgcaagaga ttatttggtt caggaacagc ctgaactgat catcaacatt
     1021 attgatgcta ccaatttaga acgaaatctt tatttaacta cgcagttatt ggatttagac
     1081 attcctatga tcattggtct gaatatgatg gatgtcatca aaaaagaagg aaaaaaaatc
     1141 aatctagaaa agctttccta tggtcttggt gtaccagttt taccaatcag cgtattgaaa
     1201 aagcaaggaa tcgaaaaact aatcgaaaag attcaggagt ctgcaaaaaa gcggccttct
     1261 attcctgttc cctgtacata tgatccacgt ttagaagcag ctttacatga aattgaagat
     1321 gtactaggag aacaaagcgg caagaaacgc tggtatgcaa tgaaatattt tgaacgagat
     1381 cagaaggtaa gagaggatta cgagatagca gtttctcagc aaaaagagat cgagcaactg
     1441 atccagttaa cagagaaact attggacgac gatagtgaga cgattctggt caatgaacgt
     1501 tatgaattta ttacccaact atgtacactc tgtgttgtgt caaatgattc tttccaatta
     1561 tcgatgagtg acaaaatcga tcagattgca acgaatcgtt ggttagcctt gccgatcttc
     1621 gcatttgtga tgtggctgat ctattatctg gctattcaaa cagtaggaac aatggggaca
     1681 gattggatca atgacacatt gtttggtaca tggttaccag aacatgttgg cagattgtta
     1741 gctgaatggc aagtagcggg atggatgcag gagctgatct tgaacggcat catcgcaggc
     1801 gtgggagcag tgttaggatt tctaccgcaa ttgattatat tgtttttatg cttgagcttt
     1861 ctggaagact gcggatatat ggcgaggatc gcttttgtca tggatcgact tttcaggaaa
     1921 ttcggtcttt caggaaaatc cttcattcct atgctgattg ctacaggatg tggagtgcca
     1981 ggcgtcatgg ccagtcgcac gattgaaaac gagcaagatc gtcgtttgac gatcatggtc
     2041 acaaccttca tgccttgttc tgcaaagttg ccgattattg ccttagtagc aggggccttt
     2101 tttcctcatc aatcttgggt agctccttct gcctattttc ttggtatgac tgcaattatt
     2161 ttctctggaa tcagcttgaa aaaaacacgt ttgtttgctg gagatacagc tccttttatc
     2221 atggaattgc ccgcatatca ttttcctcaa tggtcagcgg tcctaagaca aacctatgac
     2281 cgcagcaagt cttttgttaa aaaagcagga acgattatct ttgtttctag tatcgtgatc
     2341 tggttcagct cttcttataa tttttatgct caacctgttt ctgaagacca aagtatctta
     2401 gcctttttcg ggcgaatcct ggcagttgtt tttcagccgc ttggttgggg aaactggcaa
     2461 ggaacagtcg cagcgattac aggattggtc gctaaagaaa atattattgg cacatttggt
     2521 attttgtttg gtcatgcgga agtatccgaa aatggcagag aaatctggca agtgctacaa
     2581 cacacatata ctcccgcagc agcttattca ttgttagcct tcaatttact ttgtgcgcct
     2641 tgttttgcgg caattggggc tatccatcgg gaaatgaatg ccaagaaatg gacttggatc
     2701 gccataggct atcaatgcgg tttagcttat ttagttagtt ttgtgattta tcagttggga
     2761 cacttattgg agggtggaca ggtagcgtta gggacgtatc ttgctatttt actgatgata
     2821 ggactcgttt atgttttgtt ccgtcagcca aaatccaaga accagtttta tactatattg
     2881 tcattagaag gagaggaata ggatggcgac attcttatta agtgtgttaa tctttggaag
     2941 tgcaggcgca attgtctata ctcgtttaaa atccggtaag aattgtgatg actgtcaaac
     3001 agcttgccca gtaaaaaagg aacagatcaa gcgatgatca gtcgttgcaa gaggctattc
     3061 tccccttttt tgcttatttt gctagagcat agtaagatga gggaaagtaa aaaaagaaag
     3121 aggaaagaaa atgtctggaa agaaaaaaat cgtccgcccg cgctaccagc agattgctgt
     3181 tgatttagcc gaacgtatcg ttgaaaaccg atataaagtg ggtgagaaga tccatgcccg
     3241 ctcaacactt gccagtacgt acaatgcttc tcctgagact acccgcaagg ctatcaatgt
     3301 gctggtagat cttggaatca tggaagtacg gcatggaagt ggggcatttg ttgcttcgaa
     3361 ggaaaaagca aaagatttcc tattgcaata tcaagatgta caatccatcc aagatacaaa
     3421 agcagaaatc atggcaagtg tggagaagca acaagatgag ctgaatcatt tttctcaatt
     3481 attggatcag ctggtcaatc aaacgaagcg aatacataag atgaatcctt tacttccgtt
     3541 tgaattatct ttatcagaaa aagcaatgca tctggaccgt tcaattagcg agttgaatat
     3601 ctggcaggcg actggcgcca cagttatcgc aatttctcat aatgaagaac tgcttgtttc
     3661 acctggcccc tatgctaaat ttacggcagg ggacatcgtt cattttattg gaaatgaata
     3721 tacactacag cgtatgacaa acttttttta cccggagcat cagcttgcag aatgattgtg
     3781 tgaaatatta aaaaaatatt aacttggcta aaccttttat ttacaaagat ttagccaagt
     3841 ttttttcagt ttggacaagc aaatttcttt catttttgac aaagtgacta catgatgtta
     3901 acatagtgtg gtcacttttt tcagaaagaa aaatgaattt acataaaaaa ggagaatttg
     3961 tttggtaaaa gtagaagtaa aacatttaac aaagatattt ggtaaaaaga cacaagccgc
     4021 tttagatatg atgaatgatc accagccgaa aacggagatt ttaaagaaaa ccggtgcaac
     4081 ggttggtgtg tatgacgtga attttgacgt aaaagaagga gaaatcttcg tgatcatggg
     4141 gctttccggg agtgggaaat ccacattgat ccgtctattg aatcgtctga ttgagccaac
     4201 ttcgggcagt atctatattg atggagaaga tgtatcgaag ttaagcaaag aagaattgcg
     4261 agaagtccgt cgtcataaaa tcaatatggt tttccaaaac ttcggtctgt ttccacatcg
     4321 aacgatcttg gaaaatacag aatacgggtt agaagtccgt ggtgtaccta aagaagaacg
     4381 acaagaaaaa gcagaaaaag cattagaaaa ttctagtttg ctttcattta aagaccaata
     4441 tcctagtcag ttatctggtg ggatgcaaca acgggttggc ctagccagag ctttagcaaa
     4501 tgatcctgag atcttgctga tggacgaagc gttctcggct cttgatccgc tgatccggcg
     4561 agaaatgcaa gacgaattac tggacctgca agccaatgtt caaaaaacga ttattttcat
     4621 tacccatgat ttgaatgaag ctttacggat tggggacaga atcgccttga tgaaagacgg
     4681 cgaaatcatg cagatcggaa caggtgaaga aatattgacc aatccagcaa atgattatgt
     4741 acgtgaattt gtggaagaag tcgatcgttc caaagtgctt acggctcaaa acatcatggt
     4801 acctgcattg acgacgaata tcgaatctga tggaccaaac gttgcattga caaggatgcg
     4861 taatgaagaa gtcagcatgc tgatggctgt tgacagaaag agacacctga aagggatcat
     4921 cactgcagat caagcgttag aagctaggaa gcaaaaacgt ccattgatcg acttcctaga
     4981 tgaaaatgtg acggtgattg ggaaagacat ggtggtaagt gatattttca atattattta
     5041 tgactcaccg actccacttg cagttgtaga ggatggaaaa ttaaaaggtg tcgtcatccg
     5101 aggtagtgtg attgaagcat tagcagaaac aagcgaggtg agtgaacatg aataatctat
     5161 tgaactatca attaccagtg gcaaactggg tagaaaatat cacagaatgg tttacgacaa
     5221 ctttttctgg cctattttca tttttacaaa cgattggaca ggcagtgatg tcggggatta
     5281 caaatttact gttagtgatt ccagctcctt tgtttatctt gttattgacg gtagcagctt
     5341 tctttatttc taagaaacgt ccaggactta cgttgttcac attaatcggt ctatggttta
     5401 tttataacca aggtctatgg aatgatttga tgaatactgt cacacttgtt cttttatcta
     5461 gtgtgatctc gatcatcatt ggggtaccat tagggatctt gatggcaaaa agcagtaaag
     5521 cccaaagtat tatcaaaccg atccttgact ttatgcagac aatgccagga ttcgtttatt
     5581 tgattccagc agttgcattt ttcggtatcg ggatggttcc aggggtattt gcctcagtca
     5641 tctttgctct gccaccaacc gttcgtttta cgaatttagg gattcgacaa gttcctaaag
     5701 aattagtaga agcttctgat tcattcggga gcacaagtcg tcaaaaacta ttcaaactag
     5761 aattgccttt agctaaaagt acgatcatgg cagggatcaa tcaaacaaca atgctgtcac
     5821 tttcaatggt cgttattgcc tctatgatcg gtgcacccgg cttaggacgc ggcgtattat
     5881 cagcattgca aagagcacaa gtcgggaatg gatttgtcaa cggggttgca ttagttatct
     5941 tagcaatcat cattgaccgt tttacacagc atttgaacca gccaaacaat aaaaaagctg
     6001 caggagcaac aacacagaaa agtaaaaaac gtcaaggact gatcattggt gctgtagtag
     6061 tagtgatctt aggtgcgatt ggtataggga gtttttcatc tgccaaagaa acaaaacgta
     6121 tcaacttatc ttatgttgaa tgggatacag aagttgcttc aacaaatgta gtcggtgaag
     6181 tattgaaaca aatgggctat gatgtaacga tgacgccatt agataattca atcatgtgga
     6241 aatctgtttc aaatggcgag tcagatgcca tggtttcagc ttggctacct aaaactcatg
     6301 gttcacaata tgcacaatat aaagatcaag tcgaagacct aggtgccaat ttgacaggag
     6361 caaaagttgg tttagcagtg ccagcctaca tggatgtaaa ttcgatcgat gagttaaccg
     6421 atcaggcagg taagaaaatc ataggaatcg agccaggagc tggtgtagtt acggctgctg
     6481 aaaacacgat ccaaaaatat gacaacttga aagactggaa agtagagaca tcttcttcag
     6541 gagcaatgac cgttgcacta ggtcaagcga tcaaaaaaca cgaaccaatt gtagttacag
     6601 gatggacacc tcattggatg tttgccaaat atgatctgaa atacttagaa gatcctgaaa
     6661 acggaatggg aagcgaagag cagatccata cgatggttcg aaaaggattg aaagaagacc
     6721 agccagaagc ttacaaagta ttagacaatt tccattggtc agaaaaagat atggaaaaag
     6781 ttatgttgga aatcaacaat ggaaaagatc cacagcaagc agcaaaagac tggatcaaag
     6841 agaaccaaga attagtcgaa agctggaaaa aataaaaaga cagacacgaa ataaatggag
     6901 cttccaaaat tggatcaaat atccaaattt ggaagctcca tgttcgtctc tatctttttt
     6961 gtctataaaa aaagagaatg cgtagctcat cttcgaaacg tccaaagacg ccgacaaact
     7021 attatgaatt tatgggtgcg agtcaaagag atcgatcgta ggtatgaaag actaatgtga
     7081 ttaaagttta ttatgtacga agtgcattct caaaaaatat taaagaatat acatagttta
     7141 tatacttaaa agtgattaaa ttcttattca ggaattattt tgctacgttt caagaaggaa
     7201 ttgttttttg aaatttaatg aatgttagaa taatagtcta aaaacattaa tttaggggtg
     7261 attagattat gaaacattta aaaattttgt ctattaaaga gacacaactt atctatgggg
     7321 gtaccactca tagtggaaaa tattatggaa atggagtgta ttgcactaaa aataaatgta
     7381 cggtcgattg ggccaaggca actacttgta ttgcaggaat gtctataggt ggttttttag
     7441 gtggagcaat tccagggaag tgctaaaatg aaaaaaaatg ctaagcaaat tgttca
//
LOCUS       DAZKKW010000108         7461 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.110, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000108 DAZKKW010000000
VERSION     DAZKKW010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7461
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.110"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(65..370)
                     /locus_tag="ACG8PO_002445"
     CDS             complement(65..370)
                     /locus_tag="ACG8PO_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0207730.1"
                     /translation="MSVDKKATMKRIAELTKSESWQEDKEIVAEVQKLGKSMWTEKPK
                     RKTPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEER"
     gene            complement(367..528)
                     /locus_tag="ACG8PO_002446"
     CDS             complement(367..528)
                     /locus_tag="ACG8PO_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFQ0207731.1"
                     /translation="MTTEEVIQMRIRNIQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIRRLEE"
     gene            complement(543..902)
                     /locus_tag="ACG8PO_002447"
     CDS             complement(543..902)
                     /locus_tag="ACG8PO_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HFQ0207732.1"
                     /translation="MNKYRNKKTVHRGIKFDSIAEAEYYDLALWQAEANGWKVKLQER
                     FELMPKFELDGKKYRKIEYIPDFTFYKNGKLVKVVDVKGMQTKDFKIKAKLFCHQYQA
                     PLILAKKYRNTFKEERF"
     gene            complement(899..1750)
                     /locus_tag="ACG8PO_002448"
     CDS             complement(899..1750)
                     /locus_tag="ACG8PO_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFQ0207733.1"
                     /translation="MESLANAMEKLIRRVLVQSGKCPECSEPLYSWRAKNKDGSERCK
                     PTCMSCGYKALRVKEDIQTERIYNDSLKARALSFFQNGSVLTDKTLFKCKMENYHVVD
                     QETKIALEKAKSYTNEVLLNHPAHFILSGKSGSGKSHLSMATAWEILERSNYDKKILF
                     ISYQELLEQIKFSYNNTELRKEIEGSLIADIKTTDLVVFDDIGAELGSGVSNSRQFTN
                     NTLNTLLEARQNKATIITTNLSGPELREAYGERIVSRIFKNSEGYALKFQQTADKRIK
                     PVKGSIA"
     gene            complement(1767..2597)
                     /locus_tag="ACG8PO_002449"
     CDS             complement(1767..2597)
                     /locus_tag="ACG8PO_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer N-terminal
                     domain-containing protein"
                     /protein_id="HFQ0207734.1"
                     /translation="MAEISWIKLKTTMFDDEKIRLIQAVPESDAIIVIWIRLLVLAGK
                     TNDDGLIYIQRNMPYTEEMLATLFGKNVNTVRLALTTLANFNMIDLSSDGLIAISNWE
                     KHQNIEGMDKVRLKNAERNRKYRERKRQERLKLENDVSVTSRDGTDKDIEEDKDIDKE
                     EKKGKYSDEHLRLAKKLQSNLTEDFPKEMNKVDIEKWADTIRLMEERDKASIEAIEYV
                     INWLPTNEFWFGNIRSAKKLREKFEKLKFEIKADKKNHKKQSQKLQYSNPSEYDDLPI
                     "
     gene            complement(2600..3286)
                     /locus_tag="ACG8PO_002450"
     CDS             complement(2600..3286)
                     /locus_tag="ACG8PO_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative HNHc nuclease"
                     /protein_id="HFQ0207735.1"
                     /translation="MFKPLIDSYSAVLKKFKGNDIGATINEEVNIERLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGREISLK
                     DESNTTVSDANILANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHSEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     ETIKKLRIGG"
     gene            complement(3292..3531)
                     /locus_tag="ACG8PO_002451"
     CDS             complement(3292..3531)
                     /locus_tag="ACG8PO_002451"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0207736.1"
                     /translation="MENELSTLDQYLADPDWGKSNLQLVANKYEKSIDEAIVALTDAA
                     SITADDSKWTKRDLGILKRGVNWLEDQYREETKEK"
     gene            complement(3590..3718)
                     /locus_tag="ACG8PO_002452"
     CDS             complement(3590..3718)
                     /locus_tag="ACG8PO_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0207737.1"
                     /translation="MAREEALKIGKVIADNWWANSRSIILSKQHIEKKKAWQQIKK"
     gene            complement(3857..4045)
                     /locus_tag="ACG8PO_002453"
     CDS             complement(3857..4045)
                     /locus_tag="ACG8PO_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0207738.1"
                     /translation="MSYTLQQEHQILGLIKQRRKQLQDDRAALRKSDELSDRQAELIA
                     SELEDLRMLEIKNREIRL"
     gene            complement(4066..4350)
                     /locus_tag="ACG8PO_002454"
     CDS             complement(4066..4350)
                     /locus_tag="ACG8PO_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0207739.1"
                     /translation="MQITIPDNLVVSELTTQITNAVLNSLDERLHLMNKSVELPPYPN
                     KSEVKKVLGIGDDKLTHWISLGLKTQQWSKLDIRIERSELQRFLKENFEF"
     gene            complement(4352..4540)
                     /locus_tag="ACG8PO_002455"
     CDS             complement(4352..4540)
                     /locus_tag="ACG8PO_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFQ0207740.1"
                     /translation="MSQEFILKVRIQLAKYGKSQNWLADTIGISRPYMSDIMNGRRKP
                     DKQIKPIEAALAELEKEK"
     gene            complement(4543..5319)
                     /locus_tag="ACG8PO_002456"
     CDS             complement(4543..5319)
                     /locus_tag="ACG8PO_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HFQ0207741.1"
                     /translation="MNTPQIFNFEQHEVRTVTIHDEPFFVGKDVAKVLGYQNGSRDIN
                     RHVDVEDRQNYQNGTFESPRGLTIINESGLYSLILGSKQPNAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATKLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMRQGYTKSHTTEIPK
                     SDGGTKVVMNTKWTQKGRLFIYESLKKEGYIPEIDLLEEE"
     gene            complement(5446..5667)
                     /locus_tag="ACG8PO_002457"
     CDS             complement(5446..5667)
                     /locus_tag="ACG8PO_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0207742.1"
                     /translation="MKQNKKTPSAKGALITLNFKDQKFSAVVDEIHPQEHQKNSFATD
                     EKKTQKRSEKVLRIIQFLLEKIRHFGSLF"
     gene            5968..6312
                     /locus_tag="ACG8PO_002458"
     CDS             5968..6312
                     /locus_tag="ACG8PO_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0207743.1"
                     /translation="MLFERVKELCKKRGISISELENRIGFGKNSIYKWKTQSPKIETL
                     QKVADYFHVSTDYLLGRTDDPNAGVAPEERKLTVEEALASVMSSDGKPLTDNDREILS
                     AMIEAYLEKKDN"
     gene            6348..6743
                     /locus_tag="ACG8PO_002459"
     CDS             6348..6743
                     /locus_tag="ACG8PO_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFQ0207744.1"
                     /translation="MIINELGVKVEERENLDADGHYVACMNTIVIKANLSKYRRQRTL
                     LHELGHASKHHDNYFLYNLAFSLHSKMEYEADRFMIEKLLDRYIAKSELEPHNINYMK
                     FIEDNNLSVRFEPLVKELLKARIYCYAAL"
     gene            6796..7107
                     /locus_tag="ACG8PO_002460"
     CDS             6796..7107
                     /locus_tag="ACG8PO_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0207745.1"
                     /translation="MIYTEFKEWLEKSTTGYETFIIKATNYQIEKNKNRPQKKRWDDK
                     KIDKAVLEMWKQVVTNLYQTIRKEKGVPLINGKEIWLEFIEEQGLIEFFNDSMAELEF
                     E"
     gene            7119..>7461
                     /locus_tag="ACG8PO_002461"
     CDS             7119..>7461
                     /locus_tag="ACG8PO_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arm DNA-binding domain-containing protein"
                     /protein_id="HFQ0207746.1"
                     /translation="MAMIKQYKKKNGEKAWYFKTYLGIDPLTGKKKYTTKRGFRTQKE
                     AKTALSRLELELQKTGMPTSTNTTFKEAAELWLESYKKTVKESSYSRTKIIFNKHIYP
                     KFGNIKLSKINT"
BASE COUNT         2134 a         1426 c         1200 g         2701 t
ORIGIN      
        1 tctttctgaa tcttcatcat gattttcttg aggatgatgt cactgtattg tgtaatgaga
       61 tccattatct ctcctccaca tacctaaact gtcgtccttt tgaatcaatc cataagctcc
      121 tagctctatc ccagataatg tttttgctta atccagtaat ttcagataac tgttcagctg
      181 tacctgttac tagaattcga tcaccatgcc agattgcaat ttttctcggc gttttccgtt
      241 taggcttttc agtccacatt gatttaccga gcttttggac ttctgcaact atttctttgt
      301 cttcctgcca agattctgac ttggttaatt cagcaattcg tttcattgtc gctttcttat
      361 ccacgctcat tcctccaatc tacgaatttc ccttcttaag ttctctatgt gcaaatcgat
      421 tgcctttctc gccgtttcat tgaccatcac tgcctttgtt cgttctagat cgtcaatttc
      481 acgctgaatg tttcgaatac gcatttgaat cacttcttct gttgtcatga tggaccacct
      541 cgttaaaaac gctcttcctt gaacgtattc cgatattttt tagctaatat caacggcgct
      601 tgatattgat gacagaacaa ctttgccttg atcttaaagt cttttgtctg cattcctttg
      661 acatctacga ctttgacaag tttgccgttt ttataaaatg tgaagtcggg aatatactcg
      721 atcttgcgat acttctttcc gtctagttca aatttcggca tcagctcaaa tctttcctga
      781 agttttactt tccagccatt tgcttcagct tgccacaagg ctagatcgta atactctgct
      841 tctgcgatag aatcaaactt gatacctcga tgaacagttt ttttattacg gtatttattc
      901 atgcgatact acctttcact ggttttatgc gcttgtctgc tgtttgttgg aatttcagcg
      961 cataaccttc tgaattctta aatatcctag aaacaattct ttcaccgtag gcttctctta
     1021 gttcaggacc agataagttt gttgtgatga tcgttgcctt gttctgtctg gcttctaaga
     1081 gcgtgtttaa cgtgttgttt gtaaactgcc tactatttga taccccacta cctaattcag
     1141 ctccaatatc gtcaaaaacc accaaatcag ttgttttaat atcggctata agcgatcctt
     1201 caatttcttt tctcagttca gtattgttat aagaaaactt tatttgctct aataattctt
     1261 gatagcttat aaaaagtatt ttcttgtcat aatttgagcg ctcaagtatt tcccaagctg
     1321 tggccattga taagtggctt tttccgcttc ctgatttccc tgatagaatg aaatgtgcag
     1381 gatggttcag cagaacctca ttcgtatagc tttttgcttt ttctaaagcg atttttgttt
     1441 cttgatccac tacgtgataa ttttccattt tgcatttaaa caaagtttta tctgttaata
     1501 ccgaaccatt ttgaaaaaaa ctcaacgctc gtgcttttaa gctatcgtta tatatccgtt
     1561 cggtctgtat atcctctttc acacgtaacg ctttataacc acaactcatg catgttggtt
     1621 tacaacgttc tgaaccatcc ttatttttag ctcgccaact atacaaaggt tcgctacatt
     1681 ctggacattt tccgctttgc actaatactc ttcttattag tttctccata gcatttgcta
     1741 ggctttccat gtgatgcatc tcctttttaa attggcaagt cgtcatattc actaggattg
     1801 ctgtactgta gtttttgact ttgcttttta tgattcttct tgtctgcttt gatttcgaat
     1861 ttgagcttct caaatttttc tctcaatttc ttagcacttc taatatttcc aaaccaaaat
     1921 tcatttgtag gtagccaatt gatcacatac tcaatcgctt ctatagatgc tttatctctt
     1981 tcttccatca acctgattgt gtctgcccat ttttcgatat ctactttgtt catttctttt
     2041 ggaaaatctt cagttaaatt actttgcaac tttttagcaa ggcgtaagtg ttcgtcagaa
     2101 tacttacctt tcttttcttc tttatctata tctttatctt cttctatatc tttatctgta
     2161 ccgtcacgtg acgtcacgct aacgtcattt tccaatttga gacgttcctg tctctttctt
     2221 tccctgtatt tacggtttct ttcagcattt tttagcctta ctttatccat accctcgata
     2281 ttttgatgtt tttcccaatt actgatggca attagtccat cactgcttag atcaatcatg
     2341 ttgaaatttg ccaatgtagt tagcgctaag cgaaccgtat ttacgttttt gccaaacaat
     2401 gtagcaagca tttcttcggt ataaggcatg ttcctctgga tatatatcag accatcgtcg
     2461 ttagtctttc ctgctaaaac tagtaatcga atccatataa cgatgatggc atccgactca
     2521 ggaacagctt ggattaatcg tattttttca tcgtcaaaca tagtagtttt aagtttgatc
     2581 caacttatct cagccaaatt tatcctccta tccttaactt tttaattgtt tcctggttta
     2641 acttgatccc tttgatttga tatttatttt tgaaattgat cacacctatt ttgtgtttct
     2701 ctgtgtgatg gattctgcag agtgctgcaa atgtgtactc tgaatgatca acttctttgc
     2761 gctttcgtct tcccagcgct ttgtcaaagt gatcgatatc agctcctgtt ttgccacaga
     2821 tgcagcagac tctttttgta atgcatttgt agaagtaata ctcttgattc gctggtaaaa
     2881 tctcatatcc ttctttgaaa ggaatatgat gttcaaagat gaaatctagg atgatattcg
     2941 ctaagatatt ggcatcactc acggttgtat tcgattcgtc tttgaggctt atttcgcgcc
     3001 ctgtgacacc ttcaaaacgg aagtagaaga attccttcca gaagtccgtt ggcatgcctg
     3061 tatcgatgaa aatatcgcct atgagcgcat agatgaagtt tctttgctgt actgtgaacc
     3121 gtcgtggatc aataaagcga atttcaatga ctcgatcgcc atcatatccg tcatacatcg
     3181 ttttcagccg ttcgatattt acttcttcgt tgattgttgc accaatgtcg ttacctttaa
     3241 atttctttag taccgccgag tatgaatcta ttagtggttt aaacactcat atcacttctc
     3301 ttttgtttct tctctgtact gatcttcaag ccaattaacg cctcgtttta gaatgcccaa
     3361 gtctctcttg gtccatttac tgtcatcagc ggttatggaa gctgcatcag tcaatgcaac
     3421 aattgcttca tcaattgatt tttcgtactt gttagcaacc agttgtaaat tcgatttgcc
     3481 ccaatcagga tcggccaaat attgatctag agtggaaagt tcattttcca tatgttaaaa
     3541 tctccttagt tatgattttt tgagtgactc attgctttgg tcggcggagt cactttttta
     3601 tttgttgcca tgcttttttc ttttcgatat gttgcttgct taaaataata gaacggctat
     3661 ttgcccacca attatcagca atcactttac cgatttttag cgcttcttct cgtgccatag
     3721 ttgctccttt cttttgagtc aagcagattg attaaaacca tcaatgctgc gaacaagctt
     3781 cctccgataa tactttggtg cgctacaatc actaataacc ccaaaatgaa tcctataaaa
     3841 agtgtgtctg tcttcttcat aatctaatct ccctattttt tatttctagc attctcaaat
     3901 cctcaagttc agaagcaatt agttcagctt gtctatctga tagctcatcg gattttctaa
     3961 gcgctgcacg atcatcttgt aattgttttc tacgctgttt aatcagacct agaatttgat
     4021 gttcttgttg caatgtgtag gacataaaat cattctcctt tgcctttaga actcaaagtt
     4081 ttctttcaaa aatctttgga gttccgatcg ttcaattctg atgtctaact tgctccactg
     4141 ctgtgttttt aagcctaggc ttatccaatg tgttaatttg tcatcaccaa tgcctaaaac
     4201 tttttttacc tctgatttgt ttggatatgg aggaagctct actgatttgt tcataaggtg
     4261 taatcgttcg tccaatgaat taagaacagc attcgtaatc tgtgtagtta attcggaaac
     4321 tactaaatta tctggaattg ttatttgcat gttatttctc cttctctaac tctgctagtg
     4381 ctgcctcaat tggcttgatt tgtttatctg gttttctacg cccattcata atgtccgaca
     4441 tatatggtct agaaattccg atagtatcag ctaaccagtt ctgagacttt ccgtattttg
     4501 ctaattgaat tctgactttc aaaataaatt cttgtgacat cgtcattcct cctctaataa
     4561 atcgatttct ggaatatatc cttctttttt cagcgactca taaatgaaca gacgcccttt
     4621 ttgagtccat tttgtattca tcacaacttt tgttccgcca tcagatttcg gaatctcagt
     4681 tgtatgagat tttgtgtaac cctgtctcat gtgttttttg cataataacc attggttgcc
     4741 tacttttttc tgaataccta gcttatgaag tagtttgttc atctgttgtg gagacatccc
     4801 ataatcagcc gcgatttgac taatagttac tgaatctgta gaagataaga tactatccaa
     4861 atacgaaatt ttgggttcgt attctgcaat tttttgttct gcgattagtc ttccagtacg
     4921 ttcttctttc aacttagttg ctaattggat gattgtatct ggattaagca aagcttcttc
     4981 tactttttct ggagttagat aacctccatg ttttctaatt gctggcaaca cttcgctagt
     5041 cacccaacgt ttaaatttct tagcatttgg ttgtttgctt cctaaaatta aggagtacaa
     5101 acctgattcg ttaataatag ttaaccctct aggcgattca aaagtaccgt tttggtagtt
     5161 ttgccgatct tctacatcga cgtgtctgtt aatatctcta ctaccatttt ggtaccctag
     5221 aactttagcc acatcttttc caacaaaaaa tggttcatca tgtattgtta ctgttcttac
     5281 ttcatgttgt tcaaagttaa aaatttgcgg tgtgttcatt tttgctcatt cctttctttg
     5341 atataatttt gaatagaaag cgaggtgaaa atagtatgga agaatttaat atggatgtcg
     5401 acgccttatt caaacaaacc gtattcaaga ctgttaataa agaattcaaa atagacttcc
     5461 aaaatgacga atcttttcca acagaaattg aattattcga agaactttct cagagcgttt
     5521 ctgagtcttt ttctcgtcag ttgcaaaaga atttttttga tgctcttgtg gatgaatttc
     5581 atcaacaaca gcactaaact tttggtcttt aaaatttaaa gttattaaag ctccttttgc
     5641 ggaaggagtt tttttatttt gtttcatgat gtttcctcct tttctttaaa tatgtaagct
     5701 aataaaatta gctaattttt attgacattt ctatccaact gtcttatatt tatatacagt
     5761 taaataagcc tagaaaaacc ctaaaaatta acattcttag tttgccgacc tcgaattcgt
     5821 ttattttatt aggtgtattt cttgttgctt gttagcttat taaattagct tacgagttaa
     5881 atataagaca attggataga tttgtcaact acctttctat ctttttgtat aaattattat
     5941 cgtacgctct aggaaggttg atttgacatg cttttcgaaa gggttaaaga gctctgtaaa
     6001 aaaagaggta tttctatttc tgaattagaa aatagaatag gatttggaaa aaactctatt
     6061 tataaatgga agacacaaag tcctaaaata gaaactttgc aaaaagttgc tgactacttc
     6121 catgtatcaa cagattattt gctaggacgc acagatgatc ctaacgcggg agttgcacca
     6181 gaggaaagaa aactaaccgt ggaagaagct ttagcatctg ttatgagtag cgacggaaaa
     6241 ccgctcaccg ataatgatag ggaaatacta tcagctatga ttgaagcata tttagagaaa
     6301 aaagataact aaataagtag gtgagtcatt tggacagtca aattgaaatg ataattaatg
     6361 aactcggcgt taaggtagaa gagcgtgaaa accttgatgc cgatggccat tatgttgctt
     6421 gtatgaatac catagtaata aaagctaatt tatctaagta tagaagacaa agaaccttat
     6481 tacatgaatt aggacacgct tctaaacatc atgataatta ttttttatat aacttagcat
     6541 tctctctcca ttcaaaaatg gaatatgagg ctgatcgctt catgattgaa aaattattag
     6601 atagatatat tgcaaagtct gaattagaac cacacaatat caattacatg aaatttatag
     6661 aagataacaa tttaagcgtt cgcttcgaac cacttgtgaa agaattatta aaagctcgca
     6721 tctattgtta tgcagctctc taaaattttt taagcaaaaa agaacatacg ttcaaaaata
     6781 gaaaggtgaa caaaaatgat atatacagaa ttcaaagaat ggttagaaaa aagcacaacc
     6841 ggatacgaaa catttatcat caaagctact aattatcaaa ttgaaaaaaa caaaaataga
     6901 ccccaaaaaa aacgctggga tgataagaaa atagataaag ctgtattaga aatgtggaaa
     6961 caagtcgtga ctaacttgta tcaaacaatt cgtaaagaaa aaggagttcc attaattaac
     7021 gggaaggaaa tatggcttga atttatagag gaacaaggac tgatcgaatt tttcaatgat
     7081 agcatggcag aattagaatt tgaatagggg taatattgat ggcaatgata aaacaatata
     7141 aaaagaaaaa tggcgaaaaa gcatggtact ttaaaactta tctcggtatt gatccgctaa
     7201 ctggaaagaa aaaatatact actaaacgag gatttagaac acaaaaagaa gcaaaaacag
     7261 cactttctag gttagaacta gaattacaaa aaacaggaat gcccacaagt acaaatacta
     7321 ctttcaaaga agcagcagaa ttatggctag aaagctacaa aaaaactgta aaagaaagtt
     7381 catattcaag gactaaaata atctttaata aacatatata tcccaaattt ggaaatatta
     7441 agctttctaa aattaatacg g
//
LOCUS       DAZKKW010000109         7394 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.111, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000109 DAZKKW010000000
VERSION     DAZKKW010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7394
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.111"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            66..149
                     /locus_tag="ACG8PO_002462"
     CDS             66..149
                     /locus_tag="ACG8PO_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HFQ0207747.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            274..1011
                     /gene="erm(B)"
                     /locus_tag="ACG8PO_002463"
     CDS             274..1011
                     /gene="erm(B)"
                     /locus_tag="ACG8PO_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HFQ0207748.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNIRVTLIHQDILQFQFPNKQRYKIVGSIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            1181..1441
                     /locus_tag="ACG8PO_002464"
                     /pseudo
     CDS             1181..1441
                     /locus_tag="ACG8PO_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
     gene            1604..2269
                     /locus_tag="ACG8PO_002465"
                     /pseudo
     CDS             1604..2269
                     /locus_tag="ACG8PO_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558228.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182-like element IS1182 family transposase"
     gene            2305..2550
                     /locus_tag="ACG8PO_002466"
     CDS             2305..2550
                     /locus_tag="ACG8PO_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFQ0207749.1"
                     /translation="MIDVNELLEEAIRETENLNDGEVFLVKDLFKGYVGNRIPRKDRL
                     LLGTLFLNHVSKMNGNLKAIEKTSSNQQRYKKTIDKF"
     gene            2653..3522
                     /locus_tag="ACG8PO_002467"
     CDS             2653..3522
                     /locus_tag="ACG8PO_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HFQ0207750.1"
                     /translation="MVDNIIKSVAEKLSSLSYIEGIVLGGSRARGTHTEDSDIDIGIY
                     YNSESFDINTINQFATKLDDEHRNNLVVPPGAWGDWINGGGWLVINGYHVDLILRDIK
                     RVEQIMKDTEHGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAETY
                     PNALQKSLVNFFMFEAGFSLMFVKANSGTDDKYYIAGHVFRIVSCLNQVLFACNNAYC
                     INEKKAIKLLETFEHKPEKYTEKVNHIFEVLGISLFECYDMTEKLYNEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            3503..4237
                     /locus_tag="ACG8PO_002468"
     CDS             3503..4237
                     /locus_tag="ACG8PO_002468"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008425 -
                     2-polyprenyl-6-methoxy-1,4-benzoquinone methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0102208 - 2-polyprenyl-6-hydroxyphenol
                     methylase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFQ0207751.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            4270..5178
                     /locus_tag="ACG8PO_002469"
     CDS             4270..5178
                     /locus_tag="ACG8PO_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HFQ0207752.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            5175..5717
                     /gene="sat4"
                     /locus_tag="ACG8PO_002470"
     CDS             5175..5717
                     /gene="sat4"
                     /locus_tag="ACG8PO_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HFQ0207753.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
     gene            5810..6604
                     /locus_tag="ACG8PO_002471"
                     /note="aph(3')-IIIa"
     CDS             5810..6604
                     /locus_tag="ACG8PO_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HFQ0207754.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            6880..>7394
                     /locus_tag="ACG8PO_002472"
     CDS             6880..>7394
                     /locus_tag="ACG8PO_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFQ0207755.1"
                     /translation="MNRINRVTSILIQLQSKKIIPAKEIAQRFNISLRTVYRDIRTLE
                     EAGIPIGSEAGKGYFLVEGFLLPPVMFTAAEVGALITAGKFLNCHGDESFIKDFDSAM
                     YKIKSILKHGEKNYAQELENSINVYSTSGQKNTLADNVIAAIQTAICNKRVISIQYPA
                     SGGQEPESRMI"
BASE COUNT         2592 a         1117 c         1553 g         2132 t
ORIGIN      
        1 tgtataatag gaattgaagt taaattagat gctaaaaatt tgtaattaag aaggagggat
       61 tcgtcatgtt ggtattccaa atgcgtaatg tagataaaac atctactgtt ttgaaacaga
      121 ctaaaaacag tgattacgca gataaataaa tacgttagat taattcctac cagtgactaa
      181 tcttatgact ttttaaacag ataactaaaa ttacaaacaa atcgtttaac ttctgtattt
      241 atttacagat gtaataactt caggagtgat tacatgaaca aaaatataaa atattctcaa
      301 aactttttaa cgagtgaaaa agtactcaac caaataataa aacaattgaa tttaaaagaa
      361 accgataccg tttacgaaat tggaacaggt aaagggcatt taacgacgaa actggctaaa
      421 ataagtaaac aggtaacgtc tattgaatta gacagtcatc tattcaactt atcgtcagaa
      481 aaattaaaac tgaacattcg tgtcacttta attcaccaag atattctaca gtttcaattc
      541 cctaacaaac agaggtataa aattgttggg agtattcctt accatttaag cacacaaatt
      601 attaaaaaag tggtttttga aagccatgcg tctgacatct atctgattgt tgaagaagga
      661 ttctacaagc gtaccttgga tattcaccga acactagggt tgctcttgca cactcaagtc
      721 tcgattcagc aattgcttaa gctgccagcg gaatgctttc atcctaaacc aaaagtaaac
      781 agtgtcttaa taaaacttac ccgccatacc acagatgttc cagataaata ttggaagcta
      841 tatacgtact ttgtttcaaa atgggtcaat cgagaatatc gtcaactgtt tactaaaaat
      901 cagtttcatc aagcaatgaa acacgccaaa gtaaacaatt taagtaccgt tacttatgag
      961 caagtattgt ctatttttaa tagttatcta ttatttaacg ggaggaaata attctatgag
     1021 tcgctttttt aaatttggaa agttacacgt tactaaaggg aatggagata aattattaga
     1081 tatactactg acagcttcca agaagctaaa gaggtcccta gcgcctacgg ggaatttgta
     1141 tcgataaggg gtacaaattc ccactaagcg ctcgggaccc cttgtaggaa aatgtcctaa
     1201 gtgtggcaac aatattgtat taaaaaaatc gttttatggt tgttcaaatt atcctgaatg
     1261 taagtttact ttagctgaac attttagaaa gaaaaaacta accaaaacga atgtaaaaga
     1321 attactggag ggaaaagaaa ccctggtaaa aggaatcaaa aacaaagaga aaaagcccta
     1381 caatgccgtt gtaaaaattg gggaaaaggg atatattgat tttatctctt tttcaaaata
     1441 aacatggggc tgacccaaaa gtagctccta tatgaaaaat agaggtcttt ccatttagat
     1501 agtagtggaa agacctctat ttatcttttg aaagtgaata caaaaataaa gcacactctg
     1561 ctataatata ggtgattaat ccatttgaga gaggtgcttt atcatgtatc aaaattatac
     1621 cataaaccaa ctttgtttgc ctatagatct tgaaattaaa ttagaagaaa atgattttgc
     1681 tcatgccatt gtgcagtttg ttgactctat tcccgatgaa gtctttcttc cttattatca
     1741 atctatgggt agaccccaat atcatcctcg tatgctactt tccattattc tttgtgccta
     1801 tattcaaggg gtttattcgg gccgccaaat acaaaatatg ctgatagata gtattcggat
     1861 gcgctactta tctcaagagc aattccctaa ctttcgtact attaatcgtt ttcgcgttca
     1921 tccgattatg aacgatattc tagatcattc atttgttcaa tttcgtgagc tactggttca
     1981 aagtggctta atcagtggca gtgctctata cattgatggt acaaaaattg aagcggatgc
     2041 gaataaatac tctttcgttt ggaaaaaaag tattttaaag tataaggata gccttgatca
     2101 aaaagccctt gaaaactacc aacaaatggt ggaaacaaaa atcttacctg agttaataga
     2161 tgatttaaag gatgaacttt ctatagaaga gattgaaagg attcgtcaat ctttggaaga
     2221 aaaggaaaat caattaattg aagcgattga ccaaaccgaa acagtagaag ggggctgacc
     2281 cattattaat aaggagtgga caacatgatt gatgtaaatg agctattgga agaagctatt
     2341 agagaaactg aaaatctaaa tgatggcgaa gtttttcttg ttaaagattt attcaaggga
     2401 tatgtgggga ataggatacc cagaaaggat cgacttctgc ttggaacgtt atttttaaac
     2461 catgttagca aaatgaatgg taatttaaag gctattgaaa agacttcatc taatcagcag
     2521 aggtataaaa agacaattga taagttttaa ctatcttttg tgaaattaaa aaaattataa
     2581 atatgttgag tagaagcaaa agatttggct tggaattttt acagcagtga atcgataaaa
     2641 ggagcatgct tgatggtaga taatattatt aaatcagtag cagagaaatt atcctctctg
     2701 tcttatatag aaggtattgt tttaggtggt tcacgtgcaa ggggcaccca tacagaggat
     2761 tcggatatag atatcggcat ctattacaat tcagaatcat ttgacataaa tactattaat
     2821 caattcgcta caaagctgga tgatgagcat agaaataacc ttgttgtacc tcccggagca
     2881 tggggtgatt ggattaatgg cggcggatgg ttagtcataa acgggtatca tgtggattta
     2941 attttacgtg atattaaacg tgtggaacaa ataatgaaag atacagagca cggaattgtt
     3001 actgccaatt atcagactgg gcatccccat ggttatatta gtgcaatgta tcgaggagaa
     3061 ttagcgatta gcaaaatact atatgctaag aatgaaagct tatgcgaatt aaaaaaacag
     3121 gcagaaactt atcccaatgc tttgcagaaa agtttagtta acttttttat gtttgaagca
     3181 gggttctctt taatgtttgt aaaagcaaat tcgggaacag acgataaata ttatattgcg
     3241 ggtcatgttt ttcgtatagt ttcatgttta aatcaagtgt tatttgcatg taataatgct
     3301 tattgtatca acgaaaagaa agctataaaa ctgcttgaaa cttttgaaca taaacctgaa
     3361 aaatataccg agaaggtaaa tcatattttt gaagtactcg gtatctcact ttttgaatgc
     3421 tacgacatga ccgagaagct ttataatgaa gtgaatgaaa ttgtatcgga gataaataac
     3481 tttttaaacg aggagagttc agatgaaaga aaacaaatat gatgataata tattttttca
     3541 aaaatacagt caaatgagtc gctcgcagaa aggactggct ggtgcgggag aatgggagac
     3601 tttgaaaaag atgctacctg attttaaggg taagcgtgtg cttgatttag gatgcggcta
     3661 tggatggcac tgtatatatg cgatggaaaa cggtgcttcc tctgtagtag gtgttgatat
     3721 ttctcataaa atgctcgaag tagcaaaagg aaaaacccat tttccacaga ttgaatatga
     3781 atgctgtgcc atagaagatg tggatttccc agaggagagc tttgatgtaa tactaagttc
     3841 gcttgcgttt cattatgtag cagactatga gaatttaata aaaaagatat ataggatgct
     3901 gaaggctggt ggcaatttag tttttacagt tgaacatcct gtttttactg ctcatggaac
     3961 acaagactgg tattataacg aaaaaggaga aatactgcat ttcccggtgg acaattatta
     4021 ttatgagggc aaacggacag ctatgttttt ggaagaaaag gttacaaaat atcatagaac
     4081 actgaccaca tatctaaata cactgctttc aaatagtttt ataataaatc agattgtgga
     4141 gccacagccg ccagagaaca tgatggatat tccggggatg gcggatgaaa tgcgacgccc
     4201 aatgatgctg attgtatcgg caaaaaagaa gatgtaataa tatagaaaaa ataaacgagg
     4261 agtatgtaaa tgagatcaga aaaagaaatg atggatttag tactttcttt agcagaacag
     4321 gatgaacgta ttcgaattgt gacccttgag gggtcacgcg caaatattaa tatacctaaa
     4381 gatgaatttc aggattatga tattacatat tttgtaagtg atatagaacc gtttatatct
     4441 aatgatgact ggcttaatca atttgggaat ataataatga tgcaaaagcc ggaggatatg
     4501 gaattattcc cacctgaaga aaagggattt tcctatctta tgctatttga tgattacaat
     4561 aaaattgatc ttaccttatt gcccttggaa gagttagata attacctaaa gggcgataaa
     4621 ttaataaagg ttctaattga taaagattgt agaattaaaa gggacatagt tccgactgat
     4681 atagattatc atgtaagaaa gccaagcgca agggagtatg atgattgctg caatgaattt
     4741 tggaatgtaa caccttatgt tattaaagga ttgtgccgta aggaaatttt atttgctatt
     4801 gatcatttta atcagattgt tcgccatgag ctgctgagaa tgatatcatg gaaggtcggc
     4861 atcgaaacag gctttaaatt aagtgtaggc aagaactata agtttattga aaggtatata
     4921 tccgaggatt tgtgggagaa acttttgtcc acctaccgga tggattccta tgaaaacata
     4981 tgggaagcat tatttctatg ccatcaattg ttcagggcgg tatccggtga ggtggcggaa
     5041 aggcttcatt atgcctatcc ggagtatgat aggaatataa caaaatatac cagggacatg
     5101 tataaaaaat acactggtaa aaccggctgc ctggatagca catatgccgc tgatatagaa
     5161 gagaggcggg aacagtgatt acagaaatga aagcagggca cctgaaagat atcgataaac
     5221 ccagcgaacc atttgaggtg ataggtaaga ttataccgag gtatgaaaac gagaattgga
     5281 cctttacaga attactctat gaagcgccat atttaaaaag ctaccaagac gaagaggatg
     5341 aagaggatga ggaggcagat tgccttgaat atattgacaa tactgataag ataatatatc
     5401 tttactacca agacgataaa tgcgtcggaa aagttaaact gcgaaaaaat tggaaccggt
     5461 acgcttatat agaagatatc gccgtatgta aggatttcag ggggcaaggc ataggcagcg
     5521 cgcttatcaa tatatctata gaatgggcaa agcataaaaa cttgcatgga ctaatgcttg
     5581 aaacccagga caataacctt atagcttgta aattctatca taattgtggt ttcaaaatcg
     5641 gctccgtcga tactatgtta tacgccaact ttgaaaacaa ctttgaaaaa gctgttttct
     5701 ggtatttaag gttttagaat gcaaggaaca gtgaattgga gttcgtcttg ttataattag
     5761 cttcttgggg tatctttaaa tactgtagaa aagaggaagg aaataataaa tggctaaaat
     5821 gagaatatca ccggaattga aaaaactgat cgaaaaatac cgctgcgtaa aagatacgga
     5881 aggaatgtct cctgctaagg tatataagct ggtgggagaa aatgaaaacc tatatttaaa
     5941 aatgacggac agccggtata aagggaccac ctatgatgtg gaacgggaaa aggacatgat
     6001 gctatggctg gaaggaaagc tgcctgttcc aaaggtcctg cactttgaac ggcatgatgg
     6061 ctggagcaat ctgctcatga gtgaggccga tggcgtcctt tgctcggaag agtatgaaga
     6121 tgaacaaagc cctgaaaaga ttatcgagct gtatgcggag tgcatcaggc tctttcactc
     6181 catcgacata tcggattgtc cctatacgaa tagcttagac agccgattag ccgaattgga
     6241 ttacttactg aataacgatc tggccgatgt ggattgcgaa aactgggaag aagacactcc
     6301 atttaaagat ccgcgcgagc tgtatgattt tttaaagacg gaaaagcccg aagaggaact
     6361 tgtcttttcc cacggcgacc tgggagacag caacatcttt gtgaaagatg gcaaagtaag
     6421 tggctttatt gatcttggga gaagcggcag ggcggacaag tggtatgaca ttgccttctg
     6481 cgtccggtcg atcagggagg atatcgggga agaacagtat gtcgagctat tttttgactt
     6541 actggggatc aagcctgatt gggagaaaat aaaatattat attttactgg atgaattgtt
     6601 ttagtaccta gatttagatg tctaaaaagc tttaactaca agctttttag acatctaatc
     6661 ttttctgaag tacatccgca actgtccata ctctgatgtt ttatatcttt tctaaaagtt
     6721 cgctagatag agttctatat tagagatgta aagagttttg gtgaagaggt ttgtgagtat
     6781 ggcttttata accagggtgc aatgagaagc actaacagca gctagcttaa gaacgcatag
     6841 gatatacttt ttatggaagt cacagaggag gaataaaatt tgaacaggat taacagagta
     6901 acctctattc ttattcagct gcaatcaaaa aaaataattc ctgccaaaga aattgcacag
     6961 cgatttaata taagcttaag gacagtttac agagatatcc ggacacttga agaagccgga
     7021 ataccaattg gatctgaggc cggaaaagga tattttcttg tagagggctt cctacttccg
     7081 ccggtaatgt ttacagcggc ggaagtgggt gcattgatta cagcagggaa atttttaaat
     7141 tgccatggag atgaatcatt tataaaggat tttgattcag ctatgtataa aatcaaatct
     7201 attttgaagc atggtgaaaa aaactatgca caggagttgg agaacagtat taacgtgtac
     7261 agcacatctg gacagaaaaa tacattggcg gataacgtta ttgcagcaat tcagactgca
     7321 atctgcaata aaagggtaat atcaattcaa tatcctgcat caggagggca agaaccggaa
     7381 agcagaatga taga
//
LOCUS       DAZKKW010000110         7036 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.112, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000110 DAZKKW010000000
VERSION     DAZKKW010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7036
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.112"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            348..944
                     /locus_tag="ACG8PO_002473"
     CDS             348..944
                     /locus_tag="ACG8PO_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFQ0207756.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAGEKEYMNDADRFRVFG"
     gene            complement(1002..1484)
                     /locus_tag="ACG8PO_002474"
     CDS             complement(1002..1484)
                     /locus_tag="ACG8PO_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0207757.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(1590..1787)
                     /locus_tag="ACG8PO_002475"
     CDS             complement(1590..1787)
                     /locus_tag="ACG8PO_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0207758.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            2082..2840
                     /locus_tag="ACG8PO_002476"
     CDS             2082..2840
                     /locus_tag="ACG8PO_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HFQ0207759.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            2900..3328
                     /locus_tag="ACG8PO_002477"
     CDS             2900..3328
                     /locus_tag="ACG8PO_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0207760.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            3374..3446
                     /locus_tag="ACG8PO_002478"
     tRNA            3374..3446
                     /locus_tag="ACG8PO_002478"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3407..3409,aa:Gln,seq:ctg)
     gene            complement(3596..3967)
                     /locus_tag="ACG8PO_002479"
     CDS             complement(3596..3967)
                     /locus_tag="ACG8PO_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFQ0207761.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            4182..4367
                     /locus_tag="ACG8PO_002480"
     CDS             4182..4367
                     /locus_tag="ACG8PO_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0207762.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            complement(4450..4638)
                     /locus_tag="ACG8PO_002481"
     CDS             complement(4450..4638)
                     /locus_tag="ACG8PO_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0207763.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(4662..4925)
                     /locus_tag="ACG8PO_002482"
     CDS             complement(4662..4925)
                     /locus_tag="ACG8PO_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0207764.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            5083..5340
                     /locus_tag="ACG8PO_002483"
     CDS             5083..5340
                     /locus_tag="ACG8PO_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HFQ0207765.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            complement(5411..5602)
                     /locus_tag="ACG8PO_002484"
     CDS             complement(5411..5602)
                     /locus_tag="ACG8PO_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0207766.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(5743..6063)
                     /locus_tag="ACG8PO_002485"
     CDS             complement(5743..6063)
                     /locus_tag="ACG8PO_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0207767.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            complement(6086..6520)
                     /locus_tag="ACG8PO_002486"
     CDS             complement(6086..6520)
                     /locus_tag="ACG8PO_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFQ0207768.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            6855..>7036
                     /locus_tag="ACG8PO_002487"
     CDS             6855..>7036
                     /locus_tag="ACG8PO_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFQ0207769.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNS"
BASE COUNT         2213 a         1368 c         1072 g         2383 t
ORIGIN      
        1 tacatcttta ccgaaaaatt taactaaaaa acttatttaa gaaatataaa ctaattttct
       61 ataaaagcaa gtaattttta atggcaatat cataaaaaaa agaagataga gatttaataa
      121 ttatatatca gctgtttttt tcaaataagc aaaaattcct aaaacacaac aatgaaagcg
      181 ataactaaga aaatccttac ataacgattt ttcttattaa aattgttttt tgaacttcgc
      241 ttattttttg tgagcatatc tgtttactta ctttttgtaa ggtgctatac ttcatttcgt
      301 aatcaattaa aaaaatgcaa aacgcaaatc aaaggagaaa ttttattatg acaactttta
      361 ctgacacttt gaaaaaccgc cgttcaatct atcatctagg acgtaatgtt tcactttcta
      421 atgaagaatt aacaactttg atcaaagaag caattaaaga aagcccaact gctttcaatg
      481 ctcaatcaac acgcgcagtt atccttttcg gagatgcaca tgaaaaactt tgggaaatca
      541 ctgaagaagc acttcgccca ttgactccag cagaagcttt cccaaataca caaaacaaat
      601 tagctggttt caaaaatggt tatggtacag tattattctt caaagatact gacgtggtaa
      661 aaggtctaca agaacaattt gaattatatg cagacaactt cccagattgg tcagaacaat
      721 caaacggaat cgcaacagct aatacttggg tagcattagt tgataaagga ttgggcgcaa
      781 acttgcaaca ttacaaccca gttattgacg aagcagttgc aaaagaatgg aatatccctt
      841 caaactggaa actacgttcg caattagtat tcggttcacc agaaacaccg gctggggaaa
      901 aagaatacat gaacgatgcc gatcgcttcc gcgtatttgg ataattttat ttgaaataaa
      961 aaacagaggt tgtgacaaac atcttgtcgc aacctctgtt tttattctgg atcaatcata
     1021 atatcaatct ctgcctcatc gtttaagcgt tgggacaaaa atggaactaa ttcttctaat
     1081 gatgcagaaa atttcagcat cggtcctcgc catccgtttc ggaaaaaatt catttttacg
     1141 attttagaac cttttggctg atataataca attttattga tctcagaaaa aggaacacct
     1201 tttttgtcaa agctattcaa aacaagtcct tcttctgtga ttcctctact gtccaacaaa
     1261 aaactaataa aaatcattgc tgacaagatc ccttttgcca attcatctgc tgtaggatga
     1321 ctgatgagtg tataccaaat gactagtgac actaagaagg gtatcaccaa gtagacccat
     1381 cgatttcgca cagtccgata aataatttct tttcgaacca gccaaagaaa taaaaacatc
     1441 acaacaacga ataaaaacaa tagaaccgta aagaatgtta ccatgacaac tcgcctcgct
     1501 ttttcttctc cagaaaatag gaaaagaata ctgatttacc ttctcaaaga aattggactt
     1561 gcagcacgcc gcacgtccaa ttgttttctt tattcaaatg tatcatattt tgcaacaacc
     1621 atgcttgctt tttcatctaa taattcttgg tcctctgcag cacattgggc aactttgacc
     1681 acggcgcagt ttgtcatttt tgatacaact tcaccaatga ccgtttttgt aaaaccaatt
     1741 ggtttgcatg catattgttc tcctaaatta atcatttctt ccgtcatcaa aatcctccta
     1801 aactgctgga acttttctcc acattaccac tgctatttct ttggagattt aactcattga
     1861 tttttattta cagttaaaaa tcaaccttac aggcattata gcgtactgtt ttcgttttca
     1921 caattaaaac agctgttgta tttagaagaa tttttttcaa aaaattaaaa catattcctt
     1981 aaaaactaat tatttcctac atattaaaat ataatacttc catttccaat aaaaatacat
     2041 ttataatagg aaagataaac agctgaaaag ggagatttca aatgaagaaa aaaacgttca
     2101 aaaaactatt gtttctaggg ggcacagctg ctcttttatt aagcggtaca agttattacc
     2161 tacatatcca ttctccagaa catacaatga acactgcaca aaaaagttca acacaaaaaa
     2221 cgaaaaagac aaaagagaaa gcagaagttt tactagatgt tccattagag agccagtttg
     2281 attctccttc tttagaaaat ggctgtgaag tcacttcgct ttctatgctt ttatcttttt
     2341 atggttatga aacaactaaa aaccagttag ctgagcaatt ggattacgtc cctgttttca
     2401 atgcagatgg gactcatggc gatcctaatg aaggatttgt tggtgatatc agtggcggag
     2461 actgggctat ggtcgtctat gttcccccag ttgcctccct tgctcagcaa atcgtccaaa
     2521 cggattatca cacttttcca aaaaccgatg ctcaactcga tgatatcaaa aaagcactat
     2581 ctaaaggaaa tcctgtctgg acttctgtga ctatcaattt tgaagtccca gatgaaactg
     2641 attttatgac ttggaccaca aataatggag aagtaagagt cactcctctt gttcatgctt
     2701 gtgtagtcac tggatatgac aaagaaaata tttatgtcaa cgatccctat ggtgtaaaaa
     2761 atcgagcagt accgatcaac gatttttcgg cgattttcac tgcaatgggt ggacagatgc
     2821 taactttgga gaaaagctag taattcaaac aaaaatccct tatactcaag ataccaaacc
     2881 tttgaaggga gcgtagataa tggaaaaatt cacacataaa aaaatggatc caaatgaaat
     2941 tccgattatt tttgtccgtg actgcaaggg aaatgttcaa ggtaaagtct ctattaatga
     3001 gtggaacgag cgtcgcagac ctgctaccct caatgaactg gaaatcaaat tgtatcgtca
     3061 atctcttgtt tattacgcag atcaagagta cgagaaagcc accgatttat tgaaatttct
     3121 aattgctcga acagagtata ctcgctttga atatattgaa cgtttagcaa acatctatca
     3181 tattatgaat gaacctgtca aagaatatca gttattggac accgtcttgt cagtagcaga
     3241 actgattgcc cttcctgctg gattagaaaa gaaactagta cgccgccttt tacgagtaaa
     3301 gcaacaacta tccgatcaag aaaaataact tgcgtttttt taggaaagtg ctatgattat
     3361 tttgttgaat aaatgggatg tagccaaact ggtaaggcac ttgactctga atcaagcaat
     3421 tgtaggttcg aaccctgcca tcccaatttg atgtgcttgc tgaaaaaatg aaccccctgt
     3481 aaaagaacaa tctttttgca gggggattat tgtattattt ttactacttg tatttagttt
     3541 acataattaa attatgaata ataaaatcgg gcgtattctt aatacaaaaa ctgatttacc
     3601 caataattgc taatgctttc tccggaactt tttctctagg aacttcctca tacgtttcta
     3661 tgtgtgctcc tttattgcta attttcaaat aatgattttc ttttaacaca gaaagaccat
     3721 taaattcaac cgttcgttct ttcccattgg catcagcagc tttctgcaca taccttgctg
     3781 taccatattc atttacttcc tctggttttt gtgtctttac atagacctct cctttttcaa
     3841 ctaatggatt tagctgatca agtataccag ggatttctcc ataacttcct tcagtataat
     3901 atttcagtcc aaatagcaaa ataccactaa tcgccgccag tccaatcaaa tatttaatca
     3961 ctttcatctt tctacctcct tgttatatat tcagtatatg ggtcagttaa agaaattcca
     4021 tccgtcccaa gttggagatt tttctcctac tttagaagcg agaatcgagt gtttttttag
     4081 taaaaatgat cgttatttga tattattgac gtgtggataa atcagttttt ctcgttttgt
     4141 ccatgtaaaa aatcatgttg atagcaaagg atgtgttcaa aatgaatcaa gaagaattat
     4201 ttcaaaaagt caaggaaatg attaagaacg gcaatttcga tggcgcaaaa cgatttatcg
     4261 aagaacataa agaacaatta ggaccttata aagagaaagc ccaaaatctt ttaaaagatg
     4321 taaatatcga cagtgtcaaa aacaaattta aaaatctatt caaataagta aaactttcac
     4381 ttttgtaaaa tcaaaggagg acaatgctgc tgcatcgtcc tcctttggtt cttttctgaa
     4441 tacttcatac taaaataaac gtgtcggatc gtaatgataa gtcacattgc cgttcagtga
     4501 ggcttttccc accacatatt tacgattctc caacttcagc cacgcttctc gaaaagccat
     4561 taattcttct ctttttacat caatcttttc gatttttcca tcgatcaaat cttgaataag
     4621 ttgttcatat ggattcattt ttttcctcca tctgctgtca atcaataagt tgtaaaaata
     4681 ggttcatatg cttttttggt atctagatct tctctgactt tgactgtcgt ataaataatt
     4741 ttttctactg ggatactata ataacttcct tcaccattat tgaatcggtg aacgtctttc
     4801 tttgctaaaa taccatttat tttattcgtc agtacacgtt gttcgacact gttacttaca
     4861 acatagtaaa tcacccgaat attattgatc aaatgcaaat cgatttctaa cactacatga
     4921 tccatgaaaa tcccactcct tctttcactt tgattatagc aagtttctcc tctaatttca
     4981 aaatcgatac agattttacc ggataaatcc acaaaactgc ttgcgccatt tcgcacaaag
     5041 tgttattttg tatcatggac tttatttatc ggaggcaata gtatgatcgc gatcggtcaa
     5101 tttgtttttt atattccttt ttttattatg ataagcattc ttttctatta cataaaatgg
     5161 acaaaaaaga agttttcagt tttactggcc tctttacctg ctgtatattt tacttatcaa
     5221 attttttctt ttaggcattg ggaaacaacg tctgtattaa taacacacat catcgaattg
     5281 acgctttcag taatttttct tattatttgg atctattttt tatataagaa tcagaattga
     5341 atccctcgca tgtttaaaaa gaaaacgatt aaagatgcag caaatttctg ccgatcttta
     5401 atcgtttttt ctattctgtt ataccaaaaa aatgtttgaa tcggtgaagg atcgttcctt
     5461 gttttaattc tataaattct tgtgcgatcg ggtacttttc ttccaaattt tctttgttct
     5521 tttcgatcag gcttttcgct tcttcatatt ttttatctgc acatagtgct tttacttcct
     5581 gtaatacttt tcctgcttcc atttgcctca ccctcttgcc attttattgc tttaagtttt
     5641 atttaagtta tacgcattat tctagtttca tcccaaataa ccttttttca tttttactcc
     5701 tcgaactaat cagttttcta aaggctgatt ggttcgtctt ttttattttt cctccatttc
     5761 attctgctct tctgttaatt tatctaatgc tttgattgat gctgcggctc ttgcataatc
     5821 atagctgtat aattccataa atttatcagg cagaacaaac cacgctggat gataaccgtt
     5881 agctgtgaca gggaatcctt tatcttgttg gttatctcca tcccatctca gagcaacacc
     5941 tttttttcct ttccacttta ctaaagcgat cgagtatccc ttctttgttt gtgtgccgtc
     6001 ttcaataatt gctaaaattt ctattttttc ttttggcgca cggacttctt gaggacgaac
     6061 cattttctat tcctccctta tctcattact tcacgaccaa cacactgcaa ggggcatgtg
     6121 atacgacata agaagcggtc gatccaacta atgcttgttg tatagcacct tttcctgtcg
     6181 ctcccataac gatcaaatca atattttctt gtccagggat gacattggca atcaaatttc
     6241 ttggatctcc agtttcaact attgcgtgga tctcagcaat gccgtactct ttggcatcat
     6301 tgattttttt cagcatggct acttctgttc tttgtttttc atctgcatag atttttgaaa
     6361 aggcataggc actatgggtc agctcaactt tgttgatgat tgacacgata aataagtggg
     6421 cttcattgtc ctttgctaag tcaagggctt cagcgaacgc tttgatcgat tgtcttgatc
     6481 cgtcaactgc taccatgatg ttctggtatc ttttactcat tggaaccgcc tcctttttct
     6541 tttattgtaa catctcaagc aaaaatacaa aaaatatacg caatatgtaa ataaaatgat
     6601 gaacaaaagc aatttctttc aagttattta tacatttctt ttctcatcag aacacctgtt
     6661 ctaatcccat tatttctgca ttatgtgaaa ttcctcatca tttttcacat cgagcgaaca
     6721 tcattctgtt acaaaaaacg tggatttgtc ataaattgtc atgcttcttt tacctatgaa
     6781 atattttcac gtgttaaagt attcctcgtt gaacacaaca aataaaactt tttaacgaat
     6841 aaaggagatt tttcatgaca tcacttaaaa ctttactttt tggaacaact ttggctgccg
     6901 gcgctgcatt cttcatggga acgactgctc atgcggacga agcttatacc gttcaatcag
     6961 gtgatactct atcaacgatt tctcaaaaat acgttggtga taactccttg atcaatgcca
     7021 ttgcagaatc aaattc
//
LOCUS       DAZKKW010000111         6892 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.113, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000111 DAZKKW010000000
VERSION     DAZKKW010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6892
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.113"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            119..637
                     /locus_tag="ACG8PO_002488"
     CDS             119..637
                     /locus_tag="ACG8PO_002488"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFQ0207770.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
     gene            765..1448
                     /locus_tag="ACG8PO_002489"
     CDS             765..1448
                     /locus_tag="ACG8PO_002489"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HFQ0207771.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            1757..1830
                     /locus_tag="ACG8PO_002490"
     tRNA            1757..1830
                     /locus_tag="ACG8PO_002490"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1791..1793,aa:Arg,seq:tct)
     gene            1989..2348
                     /locus_tag="ACG8PO_002491"
     CDS             1989..2348
                     /locus_tag="ACG8PO_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HFQ0207772.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            2471..4102
                     /locus_tag="ACG8PO_002492"
     CDS             2471..4102
                     /locus_tag="ACG8PO_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HFQ0207773.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(4164..4805)
                     /locus_tag="ACG8PO_002493"
     CDS             complement(4164..4805)
                     /locus_tag="ACG8PO_002493"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFQ0207774.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     misc_feature    4991..5067
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            5087..5395
                     /gene="rplU"
                     /locus_tag="ACG8PO_002494"
     CDS             5087..5395
                     /gene="rplU"
                     /locus_tag="ACG8PO_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFQ0207775.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            5422..5766
                     /locus_tag="ACG8PO_002495"
     CDS             5422..5766
                     /locus_tag="ACG8PO_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HFQ0207776.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            5779..6072
                     /gene="rpmA"
                     /locus_tag="ACG8PO_002496"
     CDS             5779..6072
                     /gene="rpmA"
                     /locus_tag="ACG8PO_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFQ0207777.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            complement(6165..6892)
                     /locus_tag="ACG8PO_002497"
                     /pseudo
     CDS             complement(6165..6892)
                     /locus_tag="ACG8PO_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343416.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT         2129 a         1125 c         1419 g         2219 t
ORIGIN      
        1 aaatagaatt agaaacatat aattattaaa gggaaacatt tttacagcta taatttgaaa
       61 aaaatttatc gttattatat gattaagaag gattgaataa agaaagaagt tgaaaacgat
      121 acctgattac aaaaaaataa cttttataag ttctataacg ttgattatat ttttaattct
      181 tattcttctc gtcgtcttgg ttccccgaat ttttggtttg actccttata ttattgagga
      241 caaagaaatg agtccatatt atgcacaagg aaatctgata tatgtacgac cgaaaaatac
      301 aaatgagatt ttagtcggag atattattac atattatgaa aattcaggaa aaaaaatctc
      361 gacgcgtcgc gtaatagccg ttgaaaacaa ccacgaagat ttttatacaa aagctgatac
      421 aaagacttat attgagcctg gagtagtaaa aaagagaaat attatcggta gtcccatctt
      481 tcagattcca tatattggac tagtcctttc aaaaacggca tttgcatgga ttcaggggtt
      541 attctttacg attgcttttt gcctaacagg aatcacagca tggaatacct ttgtagagtt
      601 gaaaaagaag cggagacgaa cacagaaatt tttgtaagaa tgttaaaaac ataaatgtat
      661 tccagagatc caacgactct tctgaacatg aaacacgaga aaacactcat gcagcttgta
      721 gatttagtca gttgttcgta taatgtgtga agaggaggag tagtatgcgc aatgaaatga
      781 tgcatcgtcc tgtagtcaaa gaagaactat tggattttat gcgtacccag caaaaaaaat
      841 taccgggtga gctaggcaaa ctggaagaag aagctcatgt agcagaagta ccagttattc
      901 cccatgaaac agttgtattt ttgaaatttt tacttggaca gcttcagcct gaacgaatct
      961 tggagatcgg agcagctatc ggtttttcat caagtgtcat ggccacgaca atcggagaaa
     1021 acgctcatgt tacgacaatt gatcgtttcg atgtcatgat cgagaaagct aaaaaaaact
     1081 atgaacgtct aggactgaca gataaagtga ctttgcttga aggtcaagca gcagatatct
     1141 tgccggagtt gtctggtccg tatgacttta tttttatgga tagtgcaaaa tcaaaatata
     1201 ttgaattttt acctgaatgc ttacgtttgt taagaaaagg tggcgttttg atggtagatg
     1261 acatcttcca aggaggaacg atcttgctcc cagatgaaga gattccccgt ggcaaacgag
     1321 ctatccatcg taaattaaat gaatttcttc gtgttgtgat ggatcatccc gatttgacat
     1381 cgacaatctt gccattaggt gatggtgtga tcttaatgac aaaggaatca gaaacaatcg
     1441 agttgtgata catgattatg gcagcctgta gataatcgat tgaatcgagg gctcaattcg
     1501 aacttgatgt aaaagtttct atatttgtaa gtcaattaaa ggatacagga aatctaaata
     1561 aacaggagta gcatgaagtc aatgtagctc ctgttttcaa ttgataacct tagaacagct
     1621 gaaggagaat gtctcgtttt aaaagattga tttagtagtt gggccaaaat aattcattat
     1681 ttgctaatta aataactatt tcgtattgac agaagcaatt taattatcta taattattga
     1741 tgttccgctg attcttgtca tagtagctca gctggataga gcatccgcct tctaagcgga
     1801 cggtcggggg ttcgaatccc tcctgtgacg ctaaaaaacc gttagaaact gaggtttcta
     1861 acggtttttc tttgtttttt gtgccaatat tgtttgtttt caaaggcaaa taaaataaaa
     1921 aaacatcctt atcgagtaca attataagga gcaaaaaaat aaaactcatc agaaaagagg
     1981 aagaagatat gacgatacta tctagaattt tagtgactct agttgcaatt gaattttttt
     2041 atatcatgta tatcgaaacg gtgagaactg attctgacac gacaagtagg gtttttaaaa
     2101 tgagtaaaga agagttaagc agaaaatctg ttcaaacatt gtttaagaac caaggagtgt
     2161 acaatggact attaggagtg ggactacttt atggagctta tctttcttcc gcatctaaag
     2221 aaatcacaag tatgctatta atatctattt tttttgttgc attatatggc agtttgacta
     2281 gcgatcgttg gattttactc aaacaaggtg ggctagctct gttggcattg ctttctattt
     2341 tcttataaat atagaaaaaa taagaattta tctaacagga aactttgtga aaaatattac
     2401 tttatctcat gaaatatgat attctaatga atggtatttt agaaaatttc atgaaaaagg
     2461 agttagatag gtgttcaaaa attatgtttc ctcactaaaa aaagaatttt caggttatga
     2521 tctttcaaga tttcagaaag atcttttggc tgggatcaca gtagctgctg tagcattgcc
     2581 tttagccttg gcttttgggg tatcaagcgg agctgatgcg gccgcaggtt tgattacggc
     2641 tatcattgcc ggtatcgtta ttggcggttt atcaggcggt ttttatcaaa tatcaggtcc
     2701 gacaggtgcg atggcagcta tcttgatgtc tattgcggca acaaagggaa tgcaaggtgt
     2761 attgcttgct acttttttag cgggtctatt tttacttgca gcaggacttt tccgattagg
     2821 aacactgact tcttttatcc cagcacccgt aattacgggg tttacttcag gtattgctat
     2881 cattattgca ttaggtcaga tcgataattt atttggtgtt cactcggaag gtacaaacgt
     2941 tattgaaaaa ctaagtagtt atcaaacgat aggatttccg ataaacacag cctctttgat
     3001 catgggaggg ctagtcattt tagggatgtt tatctatcca aaaaaatggg cgaaacgaat
     3061 gcctagttcg ctgctggcta tcattttggc aacagcagtt atgctattgt tccatttacc
     3121 agtagcaact gtgggtaaga ttccacaaac attgatcagt agtaatcgtt tgaatatggg
     3181 agattttagc ttttctgcct tgcaacaagt ggcagttcca gctattagca ttgctttatt
     3241 aggaatgatt gaaagcttgc tatgtggtgc ttcggctggc cgaatggcaa ataagccctt
     3301 agatagcaat caagaattaa tggcgcaagg aattggaaat ctgttgcttc ctttctttgg
     3361 cgggattcct gctactgcgg ccatcgcccg tacaagcgtg gccatcaaat ctggtgcaca
     3421 aactagatta gctggtatga tacatgcagt tgtcttactg ctctccatgc ttgtttttgc
     3481 gccagtgatg tcacagattc caatgccagc acttgcaggt gttttgattg tgacggcttg
     3541 gcgtatgaat gaatgggaaa cgatcaagga gctatttact aataaatatt ggtcagcagt
     3601 tctattgttt atccttacga tgatttgtac agttatcttt gatttaagta ttgctatcgt
     3661 gatcggggtg atcggcggct gtgtattttt tgttgtcaaa agcgctgcta tcacgatttc
     3721 attagaaacg atcgattggc aaagaatggg gctgccagaa acggaaaagc tgaataactg
     3781 gacagtagtg tatattagtg gtccactgtt ttttatgtct tcagaaaaac tcaaaaataa
     3841 attggaagaa ctaaatcatc acgatggtgt cattttctcc atgcgaggcg taccaagtat
     3901 tgatttgacg gcacgtgcag tttttgatga atttcaagaa aaagcagagc aaagaggaca
     3961 aacgattatc tatacttcct tgcagccaga agtagaaaaa caactttctc acttgtggga
     4021 acatacattc acgcatcaac atcaaacagt tgctcatgcg ttgacagctt tacatgcgaa
     4081 atatactgca attagccatt aaaacaatga aaaagaacag acaagactgt aaactgaaga
     4141 ttttatggtc ttgtctgttc tttttaatat tgctgtaaaa gtgaagctag tacttgccag
     4201 tcttgatgga taacaggtac aagggagcga ttgtagaaaa tagctgctgg atgaaaaagt
     4261 ggaagaattc gatagttttt ttctgaccat tcgtaggcat ccttttgttc tgttaattgt
     4321 aagacaggcc ctgtatagag ttgaccatga tttttggtaa ccgtatattc tttgccaagt
     4381 aaacgttgaa gaccaatgtt tccaagtgtc gcaattaatt ttggctggac cttcatcagt
     4441 tcgtaatcca atattggtgc atgcgcaaat acttcggaac gagtaggtgt ccgatttgga
     4501 taaacagttt ctgactgttg tgtccgttta ttgatccgat gagtcacgcg atacgggcgg
     4561 ctccttacag cactggtgat atacacctct tctcgtgtta agccagttga agaaagtgcc
     4621 tgcatcaatt cttttccagc ttgaccgcta aaaggaacat gcgtatcaat ctctgttttc
     4681 cctggggctt ctcccacgag catcagttta ggatgaagag gtccttgccc agcgataaat
     4741 ccttctacag gacggccgtt cattcttaaa gaagcttctt gtatcagttc ttttgggtat
     4801 tccatttatg ctcctccttt ttcttttatt ataagaataa aagaaacaca atgcgactat
     4861 tttatttggt agaactgtga ggatagaaaa gcagaagtat tgcttgaatt tttgttttac
     4921 tttttgattt taaatttagt tgactaacgg ttgctgttct ggtaagctag tactgttgta
     4981 attgtggact ccacagctac aaccgctcag aacaagtaat aagcacagca tatgctgtca
     5041 cttcggatgg cgagtctaag tatatcagaa ggaggtgctt tatagcatgt acgcaattat
     5101 taaaactggt ggtaaacaag taaaagttga agtgggacaa gcaatctatg ttgaaaaact
     5161 tgacgttgaa gcaggagaaa aagttgtttt tgacgaagtt atcttagtag gcggagaatc
     5221 tacaaaagta ggcgctccaa ctgttgcagg tgcaactgtt gaaggtactg ttgaaaaaca
     5281 cggcaaacaa aagaaagtcg ttactttcaa atacaaacct aaaaaacatt cacaccgtaa
     5341 acaaggtcat cgtcaaccat atacaaaagt ggttatcgac gcgatcaacg cttaatagcg
     5401 tttgagaaag aaggcctata tatgatcaaa ggaacattta agcgaaatga cgcaggtcag
     5461 atcgtttcat tcactttgac cgggcatgct gatgctggtc cttatggaag tgatatcgtt
     5521 tgtgcgggtg tttctgcgtt agcgatcagc actgtcaacg ggattgcctc attagccggt
     5581 tttgagccga ttgttgaaat gaatgaagaa gaaggcggtt atctttatac cgaagtcact
     5641 tcaggaatga cacaagaaca aaacaatatt gctgagattc tattagaaaa tcttttatta
     5701 ggattacaat cgatagaagc tgaaaactca gaatatatcc aaattaaaac aattaatgat
     5761 aaataggagg tgcagaccat gttgttatct atgaatctac aattatttgc ccacaaaaaa
     5821 ggtggcggtt ctacatctaa cggccgtgac tcagaatcaa aacgtctagg tgctaaacgt
     5881 gctgatggac aaacagttac aggtggatca attctttacc gtcaacgcgg tacaaaaatc
     5941 tatccaggtg taaacgtggg tatcggtgga gacgacactt tatttgcaaa agttgatggc
     6001 gtagtacgtt tcgaacgtaa aggccgtaac aaaaaacaag tgtctgttta tccagtagct
     6061 caagaagctt aataaataaa ctgctctatc tcttgtgtag caagggatgg agcttttttg
     6121 taaattatgg gatttttttt gaccatactt ttgaccatac tttcttagaa gttaatataa
     6181 tttgttagtt tttgtgttgt ttcttctttt ttgctgttag taatgtgtgt atatgtgtcc
     6241 atagtagttt ttatatttgc gtggcccatt ctttcttgga cttctttgtg atctgcgcct
     6301 gcctcatata gcattgatgc atgtgtatgt ctaaaaccat gcagaccgat ataaggtaag
     6361 ccggcacgct cacgatatat attgtatctt tctgccactg actgattaat tgtgaaatta
     6421 ttttcgtcat tggtaaacaa ataattagct ttagtaaatc ctaattgaaa taagtacttt
     6481 ctttgttcga gcttccaaaa ttttagttac tttatagttt tttcatctaa ataaattatt
     6541 cgatttgatt ttttggtttt aggagtttca gatatataat atttatctga acgagctaca
     6601 gtttttttaa tggccatttc atttgtttta aaattaatat tgtcccaagt gagtgctaat
     6661 atttcgccga ttctacatcc gctgaatgct aatagtctaa agagcgtata gtctctgttt
     6721 ttaaaatatg gattctgttc ttgagatact tcatttagaa ataattccaa ctgttctttt
     6781 gtatataatt ttaatttctt ttctgtttta atttgtggct ttggaataag taaatttaat
     6841 gttggattat cagatgttaa accaatattt atagcatact taaaaacttt at
//
LOCUS       DAZKKW010000112         6816 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.114, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000112 DAZKKW010000000
VERSION     DAZKKW010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6816
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.114"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            8..81
                     /locus_tag="ACG8PO_002498"
     tRNA            8..81
                     /locus_tag="ACG8PO_002498"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:42..44,aa:Asn,seq:gtt)
     gene            121..209
                     /locus_tag="ACG8PO_002499"
     tRNA            121..209
                     /locus_tag="ACG8PO_002499"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:155..157,aa:Ser,seq:gga)
     gene            224..295
                     /locus_tag="ACG8PO_002500"
     tRNA            224..295
                     /locus_tag="ACG8PO_002500"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:257..259,aa:Glu,seq:ttc)
     gene            311..383
                     /locus_tag="ACG8PO_002501"
     tRNA            311..383
                     /locus_tag="ACG8PO_002501"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:344..346,aa:Val,seq:tac)
     gene            393..465
                     /locus_tag="ACG8PO_002502"
     tRNA            393..465
                     /locus_tag="ACG8PO_002502"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:426..428,aa:Asp,seq:gtc)
     gene            501..576
                     /locus_tag="ACG8PO_002503"
     tRNA            501..576
                     /locus_tag="ACG8PO_002503"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:534..536,aa:Phe,seq:gaa)
     gene            578..658
                     /locus_tag="ACG8PO_002504"
     tRNA            578..658
                     /locus_tag="ACG8PO_002504"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:612..614,aa:Tyr,seq:gta)
     gene            665..738
                     /locus_tag="ACG8PO_002505"
     tRNA            665..738
                     /locus_tag="ACG8PO_002505"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:697..699,aa:Trp,seq:cca)
     gene            741..813
                     /locus_tag="ACG8PO_002506"
     tRNA            741..813
                     /locus_tag="ACG8PO_002506"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:774..776,aa:His,seq:gtg)
     gene            825..896
                     /locus_tag="ACG8PO_002507"
     tRNA            825..896
                     /locus_tag="ACG8PO_002507"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:857..859,aa:Gln,seq:ttg)
     gene            942..1012
                     /locus_tag="ACG8PO_002508"
     tRNA            942..1012
                     /locus_tag="ACG8PO_002508"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:974..976,aa:Cys,seq:gca)
     gene            1033..1117
                     /locus_tag="ACG8PO_002509"
     tRNA            1033..1117
                     /locus_tag="ACG8PO_002509"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1067..1069,aa:Leu,seq:caa)
     gene            1179..1251
                     /locus_tag="ACG8PO_002510"
     tRNA            1179..1251
                     /locus_tag="ACG8PO_002510"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1212..1214,aa:His,seq:gtg)
     gene            1261..1332
                     /locus_tag="ACG8PO_002511"
     tRNA            1261..1332
                     /locus_tag="ACG8PO_002511"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1293..1295,aa:Gln,seq:ttg)
     gene            1350..1434
                     /locus_tag="ACG8PO_002512"
     tRNA            1350..1434
                     /locus_tag="ACG8PO_002512"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1384..1386,aa:Leu,seq:caa)
     gene            1689..2564
                     /locus_tag="ACG8PO_002513"
     CDS             1689..2564
                     /locus_tag="ACG8PO_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFQ0207778.1"
                     /translation="MKESASCFVLSYSGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAIEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            2668..3432
                     /locus_tag="ACG8PO_002514"
     CDS             2668..3432
                     /locus_tag="ACG8PO_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HFQ0207779.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            3419..4861
                     /locus_tag="ACG8PO_002515"
     CDS             3419..4861
                     /locus_tag="ACG8PO_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFQ0207780.1"
                     /translation="MRIFNRGGIKMNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADD
                     MLNIPVIGAAGNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMD
                     PEVNMGIFGGIISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAIFLG
                     FIWPLIQIGIDNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSP
                     SKEVFRGEMTRFINGDPQAGLFLSGFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMG
                     SGAGTSFIANITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVD
                     YLINFRIATNAILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEET
                     ELGLASKNYEYMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISG
                     IIKPTEKNYQIIIGLEVTYVMAEFNKLLEE"
     gene            4872..6202
                     /locus_tag="ACG8PO_002516"
                     /pseudo
     CDS             4872..6202
                     /locus_tag="ACG8PO_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361883.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
     gene            6215..6673
                     /locus_tag="ACG8PO_002517"
     CDS             6215..6673
                     /locus_tag="ACG8PO_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFQ0207781.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
BASE COUNT         2140 a         1109 c         1504 g         2063 t
ORIGIN      
        1 atgttattcc ggcatagctc agttggtagt agcgcatgac tgttaatcat gatgtcgtag
       61 gttcgagtcc tactgccgga gttctcattt tgagaaagag tattgagctt ctttcactct
      121 ggagagttgt ccgagaggcc gaaggagcat gattggaaat catgtagatg gtgcaaactg
      181 tctcaagggt tcgaatccct tactctccgt agcaaattta cctggcccgt tggtcaagcg
      241 gttaagacac cgccctttca cggcggtaac acgggttcga atcccgtacg ggtcatttta
      301 attgaatgtt ggaggtttag ctcagctggg agagcatctg ccttacaagc agagggtcag
      361 cggttcgatc ccgttaacct ccataatgaa ctggttccgt ggtgtagggg ttaacatgcc
      421 tgcctgtcac gcaggagatc gcgggttcga ttcccgtcgg aaccgtcgtt cttgttattc
      481 aattaataat tttaaaatat ggcgcggtag ctcagttggt agagcaacgg attgaagctc
      541 cgtgtgtcgg cagttcgatt ctgtcccgcg ccaccatgga ggagtagcga agtggctaaa
      601 cgcgacggac tgtaaatccg ttccttaggg ttcagtggtt cgaatccact ctcctccatt
      661 ttataggggc atagtttaaa ggtagaacag cggtctccaa aaccgttagt gtgggttcaa
      721 ttcctgctgc ccctgccatg gcgaatgtgg cgaagtggtt aacgcaccgg attgtggctc
      781 cggcattcgt gggttcgatt cccatcattc gcccttttta ttcttggggt atagccaagc
      841 ggtaaggcaa gggactttga ctccctcatg cgttggttcg aatccagcta ccccagtttc
      901 tataatttat tctttatttt gtagattgat aatagtataa tggcggtata gccaagtggt
      961 aaggcagagg tctgcaaaac cttcatcacc ggttcaaatc cggttaccgc cttagtacta
     1021 ataaatcaac ttgccggcgt ggcggaattg gcagacgcgc tggactcaaa atccagtgcc
     1081 cgttgagggc gtgccggttc gaccccggcc gccggtattg actaagatgt cttcatctta
     1141 gttttttatt gagaaattaa ataacatctt ttatgatggc gaatgtggcg aagtggttaa
     1201 cgcaccggat tgtggctccg gcattcgtgg gttcgattcc catcattcgc cctttattct
     1261 tggggtatag ccaagcggta aggcaaggga ctttgactcc ctcatgcgtt ggttcgaatc
     1321 cagctacccc agttattatt tttaaatttg ccggcgtggc ggaattggca gacgcgctgg
     1381 actcaaaatc cagtgtccgt tgaggacgtg ccggttcgac cccggccgcc ggtataatta
     1441 tcaatatagc aactttctta tagagagttg ctatattttt tttgttttta tgtaattacc
     1501 tccaaatcaa ttcgatacaa gtattatcat aaaacgagta aaaaagatat tgataacgct
     1561 tttattatgt gttaaactat ttttgtaaat tatgtagggt gctactattc aattaggcac
     1621 gacctaagaa agggttcttt cttaggtcgt tttttgtctt taaaaattgt ttggagacaa
     1681 aaatatgtat gaaggagtct gcaagttgtt ttgttttatc atatagtgga ggtgaattaa
     1741 cggtgtatgt gatgaaaaag ctgaatcata atgctgtatt gatcgatgac gatggaatag
     1801 aaaaaattgc gatggggaaa gggattggct tttctgctaa agtgagtaag ccatttgatg
     1861 ttgaggatgc tgacaaatta tttgtattag attctaaaga aaagatcaaa cgattcagtg
     1921 agatagtctc gcagattcca atcgaattta tggattttgc ggaagaagtg attgaactga
     1981 taacagctag gatctcctct ccattagatt caaatatcta tattacattg acagatcata
     2041 tttattttgc tattcaacga aaaagagaag atagagatgt cacagcaatt atgttgccag
     2101 aaatgagatt cctttatcca aatgaatatg atatagcaat agaagtggtc gagcgaatca
     2161 atcatcgata tcaaaccgag ttagggtcaa atgaagtagg attcatcact atgcatatca
     2221 ttaatgctga gctaggagag cctaatagtt taaagagctt aaaaatagta gaaatgacga
     2281 ccacgatttt gtcccatcta gaaaaatcct attcaaacgt ttatgataaa gaatcactga
     2341 cttatcatcg cttaatggtt catatcaaat tcctggttaa acgcttgatt tatcaggaag
     2401 aaacgccagt agaaaatatt ggttttttta ctgaaacatt taaacatagc aaagcgtatt
     2461 gtacagcact aaagattaaa gatatcatcg aacagcaatt tcaacttgcc attcaagaaa
     2521 atgaaatcat ctatttggca attcatctag cacgaatcta ttaaaataag ggcgtaacta
     2581 tctataggcg caaccaaatg acacatagta aatggactat gattatttgg ttgcgcctat
     2641 tttagtagaa aaaagaagga gaatgaaatg ggaaaagtga tttttaattc ggatgatttt
     2701 ggctatagcc atggtgtgaa ttatgggatt atggatgctt atcagcgagg aattttgaca
     2761 tcaactacat tgatggcaaa tatgccgggt ttcgagcacg ctgtgaaact aagaaaggaa
     2821 atgcctaggc taggtgtagg agtccattta acattgacgt gtggcaaacc acttctcaaa
     2881 actgtcgata ctcttatcga aagcggagag ttcagatcct tatcttatta tcagcatcca
     2941 tttaacattg acaaagatca actgtatcag gaatggaatg ctcagattca aaaaatttat
     3001 catgcgggga tcataccaac tcatttagac agtcatcatc atactcatac tttggatgaa
     3061 aaccaggagg tagttgttac attagcaaaa aaatatgatt tgccggttcg gagcaacttt
     3121 gaacgaaaag atgaagttcg tcatgtcgat tattttgagc cttgtttcga tgtcgtagga
     3181 gaaactgatg aaaaaaaacg acaaatcaat acgagtgtgg aagaatattt ggcaagttta
     3241 ctcatgatat tgagagaaga taaaacgatc gaagtcatgt gtcacgcagc gtatttggat
     3301 caggaattgt taaatggttc aagttttgtg tatcctcgta ctcaccaagt cgaattttta
     3361 attcattcag catttgctca aaaagtgaaa aatgatcctt tgattgagtt agtaacatat
     3421 gaggatattt aatagaggag gaataaaaat gaatgttaaa tcaaatgtgc agaagcttgg
     3481 aagagcgatg ctacttccag ttgcagcgat gccattagcg ggattgatca tgcgtttatc
     3541 agctgatgat atgctgaaca tcccagtaat tggtgcagct ggaaatgccg tttttggaaa
     3601 cttggatatc ttatttgcta taggagttgc aatcggtttt gtcaaaaata aggacaaagg
     3661 aatacctgca cttactggtt ttttagcgat tgctaccttg aaaaaaggct tagaaatcat
     3721 ggacccagaa gtgaatatgg ggatttttgg tggaattatt tccggtttag tagcggcatg
     3781 gacgtataat cgctttaaag aacaaaaatt accagtagtt ttttcttatt ttgcgggcga
     3841 aaaattccca ttgactatgg tgatgattct acaaacaatc gcagctattt ttttgggctt
     3901 tatctggccg ctcatccaaa ttgggattga taattttgca aaactgctcg ttgatatggg
     3961 ggcattaggt gtggggatat ttatgttttt gaatcgtttg ctgatcccct ttggactgca
     4021 ccatgtgttg aatacgtata tttattacga tttaggaagc tatacatctc ctagcaagga
     4081 agttttcaga ggagagatga cacgctttat caacggagat cctcaggctg gactgttttt
     4141 atcaggtttt tttgtagtaa tgatgtttgg tataccagct atatgtctag ctatctataa
     4201 agcagcttat aaagaaaata aagagatggt caaaggaatc atgggaagcg gtgcaggaac
     4261 ttcatttatt gcaaatatca ctgagcctgt tgaatttagt ttcatgtttc tttctcctat
     4321 gttgtacgtg atccatgcac tttttgccgg tttagcaggg attgtctgtt acttattcaa
     4381 tatacgaatt ggttttacat tcggagcatg tattgttgat tatctcatca attttcgaat
     4441 cgctaccaat gctattttga ttcttccaat cggaattttc ttttttgcct tgtattacgt
     4501 tactttttat tatttgatca ataagagaaa cattcagaca ttaggacgtg aagctaaggc
     4561 agagtttggc aatgaagtaa ccttagaaga aacagagtta gggttagcaa gtaaaaatta
     4621 tgaatatatg gcaacaaaaa tgctacaagc gtttggagga aaagccaata tcctggatgt
     4681 ctatagttgt aatacacgcc tacgggtaga ggtagtggat cccaccatgg tcgaggaaca
     4741 gcgaatcaag caattaggga taagcgggat cattaaacca actgaaaaaa attatcaaat
     4801 tatcatcggc ttagaagtga cttatgttat ggcagaattc aataaattat tagaagaata
     4861 gaaaggaaaa gatgatgatg aaaaaacaga ttatcactat agctggaggc gggagtacgt
     4921 atacaccagg aatcgttcaa gccattttga acaatgaaaa acggttgcca ttatctgaaa
     4981 tacgtttata tgatattgat gaagaaagaa atatggatat gtacttgatt gttaaattta
     5041 tgctgaaaaa aaaggttttt caaatattcg cattcgtgca acggatgatc caaagcttgc
     5101 ttttacagga tgcgattttg ttttctcaca aattcgtgtt ggaggattgg agatgcgaga
     5161 aaaggacgaa aagattccac ttaaacatgg attagtagga caggaaacgt gtggactcgg
     5221 aggatttgct tatgggatgc gctcaatcaa aggtttattg gaaattgtcg atcatataca
     5281 agactatgcc cccaaagcat ggattttgaa ttatacaaat ccagagtcga tcgtttctga
     5341 agctgttcgt cgtaaatatc cgaatgtaaa aatgattaat gcctgtgata tgactatttc
     5401 aattgaagaa acgattgcag tgaattacgg ttatgatcgt aagaattgga tcgttacgta
     5461 ttatggttta aaccactttg gctggtatac atcgatttat gataaagaat tgcaacgaga
     5521 aattatgcca gagattattg aaaaactaat aacaaaagaa atgcaagtcg ccgattttaa
     5581 tataggagac aaaacttggc aaaaaacctt tcaaatgatg tcagtcatca cgaaaaattt
     5641 tccaagtaat atcccgaata attacttaga gtattactta tatcctgata tggtcgttga
     5701 gcatactgac aaagagtata cacgtgctaa tatggtcatg gatggacgag agaaaaatac
     5761 caaagaaatg gctgataaaa tacgccgcgg tattgtggaa gaagtattga actttaattt
     5821 tggggagcat ggacaatata tcgtcgatat agctatttcc ttgttgaatg atgatcgtcg
     5881 tcgctttatg ctaatcgttc ctaatcaagg ggcgattccg aatctacggt cagatgcagt
     5941 tgttgaaata ccggcatatg ttggtgcaac tggtgtggag acaattacac taagaaaacc
     6001 aatcagtgat tttcataaag gattgatgga ggcacaagtt gcagcagaga agttactagt
     6061 agatgcttac tttgaaggtt cttatcaaaa agctttacaa gcatttacat tgaatcaaac
     6121 cgtaccaaat gccagagtcg caaaaaaaat actagatgaa atgatcaaag taaacaaaaa
     6181 atattggcca gcattaaagt agaggtgata aaaagtgcat ttctttaaaa caagaaacat
     6241 tctaaaagct gtagctagtg gtcaaattat tccattaaca gcagtaaaag acgaggtctt
     6301 ttcttcaaaa atgataggag ttggatacgc tataacgaat catgatggat atatccatgc
     6361 gccagttaaa ggaataatcg ataatatatt tcctacccga catgccataa cgatcgaaag
     6421 tgaatctggc agaaaaatac tgatccatat gggtttagat acagttgaac tcgaaggaag
     6481 tccgtttcag atacgggttg aaagtaacca ggaggtaaaa gaagggacac tgttagctat
     6541 tatagatttg aagaagttgg agcaagcaca aaaagatacg gtgatcattg tagtttctcc
     6601 aggcattttt gaaggaaatt tattaaaaca aaaccaagaa gtaaagatta aagatagttg
     6661 ttttgaattt tagtgtacga tgaaaaaagt taaatagggt gatatttatg ggtgaattta
     6721 caaaattttt ttgaaatgat aagtttcaat cactagattt aatcattttg atttttgtta
     6781 attatcgaat ttttttgttg ctcctatgat ttagaa
//
LOCUS       DAZKKW010000113         6811 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.115, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000113 DAZKKW010000000
VERSION     DAZKKW010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6811
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.115"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            269..964
                     /gene="vanR-A"
                     /locus_tag="ACG8PO_002518"
     CDS             269..964
                     /gene="vanR-A"
                     /locus_tag="ACG8PO_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-A"
                     /protein_id="HFQ0207782.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            942..2096
                     /locus_tag="ACG8PO_002519"
     CDS             942..2096
                     /locus_tag="ACG8PO_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS-A"
                     /protein_id="HFQ0207783.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            complement(2194..3486)
                     /locus_tag="ACG8PO_002520"
     CDS             complement(2194..3486)
                     /locus_tag="ACG8PO_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element IS1251 family transposase"
                     /protein_id="HFQ0207784.1"
                     /translation="MSYTHLIKETLDILDLSVTFNENCLTKEKYKGQICHIYRGNLIY
                     TAQECIHCKHQIASDIVRWGTTTVRLLMNDVSEYRTYLELKKQRFKCKACQRTFVADT
                     SVAKKHCFISEKVRWSVVTRLKKNTSMTEIAAQKNLSVSSVYRIMKRFYRPLNPFRTP
                     LPKVLCFDEFKSVRGVSGAMSFIMMDGQTQRLLDIVENRQLPFLKRYFSHFSREIREA
                     VEWIVIDMYAPYVSLVKKLFPKAQLIIDRFHIVQHIGRTFRNHRIKETNQLLKSKEQK
                     HYQLGKQLKRYWKLLQKDERKLDYTRRLWRPGFKAHLTETDIVDRLLKGSPALRVGYQ
                     LYQDFLYAVKERDYVSFEELLTNNIMLPEGYQTTLRTFQKFLPQIKNALQQSYSNGPL
                     ECLNNHIKVLKRNAYGFRSFYNFKLRIMIRHGNALIFN"
     gene            3818..4786
                     /locus_tag="ACG8PO_002521"
     CDS             3818..4786
                     /locus_tag="ACG8PO_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HFQ0207785.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            4779..5810
                     /gene="vanA"
                     /locus_tag="ACG8PO_002522"
     CDS             4779..5810
                     /gene="vanA"
                     /locus_tag="ACG8PO_002522"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HFQ0207786.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALAVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            5816..6424
                     /locus_tag="ACG8PO_002523"
     CDS             5816..6424
                     /locus_tag="ACG8PO_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HFQ0207787.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPNRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT         2097 a         1276 c         1534 g         1904 t
ORIGIN      
        1 tactaatgta tctagggctt cattatacag gaaattatca gaagtgaata attagccatt
       61 ctgtattccg ctaatgggca atatttttaa agaagaaaag gaaactataa aatattaaca
      121 gcctcctagc gatgccgaaa agccctttga taaaaaaaga atcatcatct taagaaattc
      181 ttagtcattt attatgtaaa tgcttataaa ttcggcccta taatctgata aattattaag
      241 ggcaaactta tgtgaaaggg tgataactat gagcgataaa atacttattg tggatgatga
      301 acatgaaatt gccgatttgg ttgaattata cttaaaaaac gagaattata cggttttcaa
      361 atactatacc gccaaagaag cattggaatg tatagacaag tctgagattg accttgccat
      421 attggacatc atgcttcccg gcacaagcgg ccttactatc tgtcaaaaaa taagggacaa
      481 gcacacctat ccgattatca tgctgaccgg gaaagataca gaggtagata aaattacagg
      541 gttaacaatc ggcgcggatg attatataac gaagcccttt cgcccactgg agttaattgc
      601 tcgggtaaag gcccagttgc gccgatacaa aaaattcagt ggagtaaagg agcagaacga
      661 aaatgttatc gtccactccg gccttgtcat taatgttaac acccatgagt gttatctgaa
      721 cgagaagcag ttatccctta ctcccaccga gttttcaata ctgcgaatcc tctgtgaaaa
      781 caaggggaat gtggttagct ccgagctgct atttcatgag atatggggcg acgaatattt
      841 cagcaagagc aacaacacca tcaccgtgca tatccggcat ttgcgcgaaa aaatgaacga
      901 caccattgat aatccgaaat atataaaaac ggtatggggg gttggttata aaattgaaaa
      961 ataaaaaaaa cgactattcc aaactagaac gaaaacttta catgtatatc gttgcaattg
     1021 ttgtggtagc aattgtattc gtgttgtata ttcgttcaat gatccgaggg aaacttgggg
     1081 attggatctt aagtattttg gaaaacaaat atgacttaaa tcacctggac gcgatgaaat
     1141 tatatcaata ttccatacgg aacaatatag atatctttat ttatgtggcg attgtcatta
     1201 gtattcttat tctatgtcgc gtcatgcttt caaaattcgc aaaatacttt gacgagataa
     1261 ataccggcat tgatgtactt attcagaacg aagataaaca aattgagctt tctgcggaaa
     1321 tggatgttat ggaacaaaag ctcaacacat taaaacggac tctggaaaag cgagagcagg
     1381 atgcaaagct ggccgaacaa agaaaaaatg acgttgttat gtacttggcg cacgatatta
     1441 aaacgcccct tacatccatt atcggttatt tgagcctgct tgacgaggct ccagacatgc
     1501 cggtagatca aaaggcaaag tatgtgcata tcacgttgga caaagcgtat cgactcgaac
     1561 agctaatcga cgagtttttt gagattacac ggtataacct acaaacgata acgctaacaa
     1621 aaacgcacat agacctatac tatatgctgg tgcagatgac cgatgaattt tatcctcagc
     1681 tttccgcaca tggaaaacag gcggttattc acgcccccga ggatctgacc gtgtccggcg
     1741 accctgataa actcgcgaga gtctttaaca acattttgaa aaacgccgct gcatacagtg
     1801 aggataacag catcattgac attaccgcgg gcctctccgg ggatgtggtg tcaatcgaat
     1861 tcaagaacac tggaagcatc ccaaaagata agctagctgc catatttgaa aagttctata
     1921 ggctggacaa tgctcgttct tccgatacgg gtggcgcggg acttggattg gcgattgcaa
     1981 aagaaattat tgttcagcat ggagggcaga tttacgcgga aagcaatgat aactatacga
     2041 cgtttagggt agagcttcca gcgatgccag acttggttga taaaaggagg tcctaagaga
     2101 tgtatataat tttggctctt tgtcaaatga tgttggtaaa gagaaatctt agaataattg
     2161 gagatcaagt ggatcagttc ctcttgatct tttttagttg aaaatcaatg cgttgccatg
     2221 acgaatcatg atgcgcaatt tgaaattata aaagctacga aagccatatg catttcgttt
     2281 taacactttg atatggttgt tcagacattc aagtgggcca tttgaataac tttgttgtaa
     2341 agcatttttg atttgtggca gaaatttctg gaacgttctc aaggtcgtct gatagccttc
     2401 gggaagcatg atattattgg taagtagttc ttcaaacgat acgtagtctc gttcctttac
     2461 agcgtaaaga aagtcttgat ataactgata gcctacacgc aaagcagggc tccctttcag
     2521 taagcgatcc acaatatcgg tctctgttaa atgagctttg aagccaggtc gccaaaggcg
     2581 tcgagtataa tcaagcttgc gttcatcttt ttgaagaagt ttccagtatc tttttaattg
     2641 cttacctaat tgatagtgtt tttgttcttt gctttttaat agttggttcg tttctttgat
     2701 tcgatgattg cgaaaggtac gaccaatatg ttggacgatg tgaaaccggt caataatcag
     2761 ctgtgctttt ggaaacagct ttttaacgag cgatacataa ggagcgtaca tatcaatcac
     2821 gatccattcg accgcttcac gaatttctcg tgaaaaatga ctaaaataac gttttaaaaa
     2881 aggcagttgt cggttctcga caatgtccaa taagcgctgt gtttgtccat ccatcatgat
     2941 gaaactcata gcacctgaaa caccccgaac tgatttgaat tcatcaaaac aaagtacctt
     3001 aggtaacggt gtccgaaaag gattcaacgg acgataaaat cgcttcataa tacggtaaac
     3061 agaagagacc gatagatttt tctgagcggc gatctctgtc attgaggtat tttttttcag
     3121 acgagtcacg acagaccagc ggactttctc actgatgaaa caatgttttt tcgcaacaga
     3181 agtatctgcg acaaaggtcc gctgacaagc tttgcatttg aagcgttgct ttttcaattc
     3241 gaggtaggta cggtattcag aaacatcgtt cattaacaac cggacggttg ttgtacccca
     3301 acgcacaata tctgaagcaa tctggtgttt acagtggatg cattcttggg ctgtataaat
     3361 caagtttcca cgataaatat gacagatttg ccctttgtat ttttcttttg tgagacaatt
     3421 ttcgttaaaa gtaactgata agtctagaat atctaatgtt tctttgataa ggtgagtgta
     3481 agacatcttg attcctcctt ttgggttggt tcgccaaatt aattctaaag gatttcaagg
     3541 tgtcttttct atatgttttg gaaaataaaa aagatgctgg tgtcatccac caacatcaaa
     3601 aagtatagag ccataatttt ttaggaaaat ctcaaggtta tctttacttt ttcttaggaa
     3661 attaacaatt taatattaag aaacggctcg ttcttacacg gtagacttaa taccgtaaga
     3721 acgagccgtt ttcgttcttc agagaaagat ttgacaagat taccattggc atccccgttt
     3781 tatttggtgc ctttcacaga aagggttggt cttaattatg aataacatcg gcattactgt
     3841 ttatggatgt gagcaggatg aggcagatgc attccatgct ctttcgcctc gctttggcgt
     3901 tatggcaacg ataattaacg ccaacgtgtc ggaatccaac gccaaatccg cgcctttcaa
     3961 tcaatgtatc agtgtgggac ataaatcaga gatttccgcc tctattcttc ttgcgctgaa
     4021 gagagccggt gtgaaatata tttctacccg aagcatcggc tgcaatcata tagatacaac
     4081 tgctgctaag agaatgggca tcactgtcga caatgtggcg tactcgccgg atagcgttgc
     4141 cgattatact atgatgctaa ttcttatggc agtacgcaac gtaaaatcga ttgtgcgctc
     4201 tgtggaaaaa catgatttca ggttggacag cgaccgtggc aaggtactca gcgacatgac
     4261 agttggtgtg gtgggaacgg gccagatagg caaagcggtt attgagcggc tgcgaggatt
     4321 tggatgtaaa gtgttggctt atagtcgcag ccgaagtata gaggtaaact atgtaccgtt
     4381 tgatgagttg ctgcaaaata gcgatatcgt tacgcttcat gtgccgctca atacggatac
     4441 gcactatatt atcagccacg aacaaataca gagaatgaag caaggagcat ttcttatcaa
     4501 tactgggcgc ggtccacttg tagataccta tgagttggtt aaagcattag aaaacgggaa
     4561 actgggcggt gccgcattgg atgtattgga aggagaggaa gagtttttct actctgattg
     4621 cacccaaaaa ccaattgata atcaattttt acttaaactt caaagaatgc ctaacgtgat
     4681 aatcacaccg catacggcct attataccga gcaagcgttg cgtgataccg ttgaaaaaac
     4741 cattaaaaac tgtttggatt ttgaaaggag acaggagcat gaatagaata aaagttgcaa
     4801 tactgtttgg gggttgctca gaggagcatg acgtatcggt aaaatctgca atagagatag
     4861 ccgctaacat taataaagaa aaatacgagc cgttatacat tggaattacg aaatctggtg
     4921 tatggaaaat gtgcgaaaaa ccttgcgcgg aatgggaaaa cgacaattgc tattcagctg
     4981 tactctcgcc ggataaaaaa atgcacggat tacttgttaa aaagaaccat gaatatgaaa
     5041 tcaaccatgt tgatgtagca ttttcagctt tgcatggcaa gtcaggtgaa gatggatcca
     5101 tacaaggtct gtttgaattg tccggtatcc cttttgtagg ctgcgatatt caaagctcag
     5161 caatttgtat ggacaaatcg ttgacataca tcgttgcgaa aaatgctggg atagctactc
     5221 ccgccttttg ggttattaat aaagatgata ggccggtggc agctacgttt acctatcctg
     5281 tttttgttaa gccggcgcgt tcaggctcat ccttcggtgt gaaaaaagtc aatagcgcgg
     5341 acgaattgga ctacgcaatt gaatcggcaa gacaatatga cagcaaaatc ttaattgagc
     5401 aggctgtttc gggctgtgag gtcggttgtg cggtattggg aaacagtgcc gcgttagctg
     5461 ttggcgaggt ggaccaaatc aggctgcagt acggaatctt tcgtattcat caggaagtcg
     5521 agccggaaaa aggctctgaa aacgcagtta taaccgttcc cgcagacctt tcagcagagg
     5581 agcgaggacg gatacaggaa acggcaaaaa aaatatataa agcgctcggc tgtagaggtc
     5641 tagcccgtgt ggatatgttt ttacaagata acggccgcat tgtactgaac gaagtcaata
     5701 ctctgcccgg tttcacgtca tacagtcgtt atccccgtat gatggccgct gcaggtattg
     5761 cacttcccga actgattgac cgcttgatcg tattagcgtt aaaggggtga taagcatgga
     5821 aataggattt acttttttag atgaaatagt acacggtgtt cgttgggacg ctaaatatgc
     5881 cacttgggat aatttcaccg gaaaaccggt tgacggttat gaagtaaatc gcattgtagg
     5941 gacatacgag ttggctgaat cgcttttgaa ggcaaaagaa ctggctgcta cccaagggta
     6001 cggattgctt ctatgggacg gttaccgtcc taatcgtgct gtaaactgtt ttatgcaatg
     6061 ggctgcacag ccggaaaata acctgacaaa ggaaagttat tatcccaata ttgaccgaac
     6121 tgagatgatt tcaaaaggat acgtggcttc aaaatcaagc catagccgcg gcagtgccat
     6181 tgatcttacg ctttatcgat tagacacggg tgagcttgta ccaatgggga gccgatttga
     6241 ttttatggat gaacgctctc atcatgcggc aaatggaata tcatgcaatg aagcgcaaaa
     6301 tcgcagacgt ttgcgctcca tcatggaaaa cagtgggttt gaagcatata gcctcgaatg
     6361 gtggcactat gtattaagag acgaaccata ccccaatagc tattttgatt tccccgttaa
     6421 ataaactttt aaccgttgca cggacaaact atataagcta actctttcgg caggaaaccc
     6481 gacgtatgta actggttctt agggaattta tatatagtag atagtattga agatgtaagg
     6541 cagagcgata ttgcggtcat tatctgcgtg cgctgcggca agatagcctg ataataagac
     6601 tgatcgcata gaggggtggt atttcacacc gcccattgtc aacaggcagt tcagcctcgt
     6661 taaattcagc atgggtatca cttatgaaaa ttcatctaca ttggtgataa tagtaaatcc
     6721 agtagggcga aataattgac tgtaatttac ggggcaaaac ggcacaatct caaacgagat
     6781 tgtgccgttt aaggggaaga ttctagaaat a
//
LOCUS       DAZKKW010000114         6569 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.116, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000114 DAZKKW010000000
VERSION     DAZKKW010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6569
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.116"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            127..579
                     /locus_tag="ACG8PO_002524"
     CDS             127..579
                     /locus_tag="ACG8PO_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0207788.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            593..1282
                     /locus_tag="ACG8PO_002525"
     CDS             593..1282
                     /locus_tag="ACG8PO_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0207789.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            1407..3758
                     /locus_tag="ACG8PO_002526"
     CDS             1407..3758
                     /locus_tag="ACG8PO_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase domain-containing protein"
                     /protein_id="HFQ0207790.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
     gene            3838..4233
                     /locus_tag="ACG8PO_002527"
     CDS             3838..4233
                     /locus_tag="ACG8PO_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0207791.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
     gene            4558..5175
                     /locus_tag="ACG8PO_002528"
     CDS             4558..5175
                     /locus_tag="ACG8PO_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0207792.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            5216..5515
                     /locus_tag="ACG8PO_002529"
     CDS             5216..5515
                     /locus_tag="ACG8PO_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BRCT domain-containing protein"
                     /protein_id="HFQ0207793.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            5549..5716
                     /locus_tag="ACG8PO_002530"
     CDS             5549..5716
                     /locus_tag="ACG8PO_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFQ0207794.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(5761..6366)
                     /locus_tag="ACG8PO_002531"
     CDS             complement(5761..6366)
                     /locus_tag="ACG8PO_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0207795.1"
                     /translation="MGKIGYIRVSSQDQNLDRQLAMMEGLNIDKLFQEKASGKDTERP
                     EFQKLLNYIREGDCVIVTSLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGN
                     ELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQ
                     KRLVFKTVVDMLKQGIPVAEIAKENGISRPTIYKIKKANDL"
BASE COUNT         2265 a          910 c         1285 g         2109 t
ORIGIN      
        1 attcctcctg ctatcttttt ctattattct atcttatttg atagtagatt taaaactttg
       61 caacagaacc aattatctta tcgtagatat atattataaa tttaataaga aagcgaggtg
      121 agaatcgtga aaaaaacgat actaagtatt ttttcagtat ttacaatatt aattgggggc
      181 tattttcttt tgaatttaga ttctgttaat tcaaacgctc aaataaaaga tcaaagagta
      241 gctgaacata ataataaaaa aatgagtttg accaaaaata tctattctat gtcagatgaa
      301 gaattgtata aagaatttgg tgatttatcc ggggaacagt tagaagaaag tattccagga
      361 attagtgact cagataaaat tgttgtcttg tctgctggtg ggcaattaat gggagatggc
      421 aaatccgaat tagttagtta taaacaaaat cacagacagt ttattgatgt taatactgat
      481 tccaacgcta tttcgattaa ggatctaaaa gagcttttaa atagtaaaag aaatgaatta
      541 aagcagataa aagaaactaa ttcagtggaa ttagagtaaa ggaagattca ttatgaaaaa
      601 gatttttggt gtttctgttg ggctacttat gatgttaaca tttggaaatg cagtatatgc
      661 tgctgagaaa gaagttattt cagatccaga gcagtacgcc tcacgatact ttgctgattt
      721 tgaagaagaa gataaagtac ttgcattgcc agaaggtggt tttttaaaag gggaagctaa
      781 aattgtatct atggataatc cagatataat tcaaggttca tataattcag aaactgatcc
      841 tgcttcagtt tcagtgacag aagcaaaaaa aattgttgct gaaaaattca aaaatgatat
      901 tgaaactact ccaattatgc ctcgaggagc atttccaccc gatgttggta ctagcggaca
      961 ccttcttgta ctagcaggag gaggaagcta taaatcacag ccatttagtg gtgcgggatg
     1021 gcgttttgga ggtatgggtg taaaaccagc tgatggtaca ggcaactttt tacgttggaa
     1081 atcctacatt gacgatggaa gagtaggaaa tgtaaatcaa gccaacgcaa ctaaatctgg
     1141 ttctattcaa ggaatggcat tgtatgctcg tgaaggattc aaatactttg caccaaacgg
     1201 gcctggttgg acaatgaata taggtattta ttacacttat aatcctgttg caggtacgta
     1261 ttatgttgtt caaaatattt aataaaacag cacaaagaga tttctttgtg ctgttttatt
     1321 atttttagtt gttgacaaac actaaaatat aaaatataat caacacataa acgaacgtat
     1381 gttcggcaag aaagcagggg atttttatgg atgagatggt tttaagtact cagaaatggc
     1441 taaacaagaa atacagtaat gttacagggt ttgataaggt tccagaaaat gggcgaacag
     1501 ggtggcctac aatctatgga cttattgaag gactacaagt agaacttggt attactaatc
     1561 tagtagctaa ttttggacct accactgaaa aaatgtacga taatcaggtt actcctaaat
     1621 ggggcaaaaa tctacctaaa aatattgtat ttcttattca aggtgccttt tggtgtaagg
     1681 ggattaatcc aggtggcttc gatggggtat atactccata tttagatact gctgtaaaag
     1741 agttgcagac tgatgcaggg tttaatggaa gcgctgtaac tggagttttg gatgcgaaat
     1801 gggccaaagc gttatttgat atgagtgctt ttgttcttgt tcccggcgga gatagtaaaa
     1861 ttagaaaaat gcagcaatgg ttaaatgtaa attatttaga atacacggga ataatgcctt
     1921 gtgatggtat ttatcaaaga aattcaaatc aagcattaat ttttgcatta caagctgaac
     1981 taggatattc tcccagtgaa gcaacagggt cttatggtaa tgggacaact agtaaaactt
     2041 atcctgtgtc tgaaggtaac tcgggaaact atgtaagaat aattcaatat ggattatatg
     2101 ttaatggtta ttttgaagac ggtacattcg atggtatttt cactaaatat atgggtttag
     2161 aagtactggc attccgaaaa tttatggtat tgcctgaata tactgaaata gcagatgttg
     2221 ttgtaataaa aggactattg tctagcgctg gagatattag aagatctgca gatggcgcag
     2281 atacttctac gcaattaact agatctcaaa tccaaacatt ggttgataat gatataaaaa
     2341 tagttggtag atatcttact ggtactgttg gaattggaaa agatgaacga aataagtatc
     2401 taactacaga ggaattgaat aatatttttt ctaaaaattt atctgtgttt cctatctatc
     2461 aagatggagg tgcagcatta gcttatttta cttatgatcg tggcttatca gatggtaaga
     2521 aggctataga tgcagcaaaa aatctaaata taccattaac aacagttatt tattttgctg
     2581 ttgatctaga catgttaggg gaagaaattc ttgcatatgc tggagaatat tttaaggggg
     2641 tatctgctgt aatgtcatat agcgggtatc aaacgggtgt ttacggaaca agaaatgttt
     2701 gttctcaaat aatcaataac gggtatgctt cgtttgcatt tgtttcagat atgtcgactg
     2761 gttatagtgg gaatttaggt tttcctatgc ctagacaatg gtcttttgat caatttgttg
     2821 aatttaccat aggaagtggc aacggagctg ttggtatcga tttaatcgct acatcgggta
     2881 gagatagcgg atttaatgag ttatcaaatg atactaataa tgataattac attgccaaat
     2941 acaatcagaa ggttataaat cagatggtac gtgcgtatca atatctatca caagctggtc
     3001 ttgataatcc ttggaaccct catttgacat tttatagata tgtaaattat tctggtttgt
     3061 catgggacat catttcttct cctgtaacag agcatgatag aaaaatttat gatgaatata
     3121 ggaataaatt aactaatagc gagggcctgt ataattattt tatagatcct aatactggtt
     3181 caataatagg tttgcctcac ttaattgtga cgttacaatc acagatgttt ctagccgaca
     3241 caatcaatga tagtgtttct gactttgcag gttggttagg agatttaatg acttgctggg
     3301 gtgaagttaa gaaattagga atacaaatgg agcaaggtgt atttgcatta gtaggtacgg
     3361 ctgcaggtgg aacatttagt ctagaagatt tataccaaga tgttgacgcg gtaaatgttt
     3421 ataccttagc aaaaagaaat tctgattcaa cagctttatc ggctttaaaa acgtactatt
     3481 gtgataaggg tgctattgat agatttaatc aatttgttaa gaacagattt ggttcttcag
     3541 ataaaatcgc ttcagtaaca aatagtttat tagaaaatga cgaaacagtt gatatcttag
     3601 gtgtagggat aatttcattt ttcattgata aaatagttaa taattcactt gggacagatt
     3661 ttacttatct tgattttatt ggagatgagg ggaaagttgc aaatggattt tctgaaaaaa
     3721 ttattcaatt tcaagaaaat agaagtaaac ttaattaaga atcgtttact atttaaacag
     3781 tgtccttttc ctttttaaat atatacgata aaataagtat atgaattgta aaaggacatg
     3841 atgattatgg aagaaataga atggaataat ctgactcaaa ataataaaaa taattctgga
     3901 tgtggaatta tctttatgtt tgttatagtt ttgggaatag tagtactttt aataactgtc
     3961 aagatagtag attactttgc gaatactcct gaagaacaaa tacagagaaa acttcctgag
     4021 tttgaaagag taccacctta tgatccaaga gataattcat ttcaagtaaa aaaattaaaa
     4081 gagtctccaa atgaaatgtg gtttgaagtt acagttgaat caagcgactt caaagaaaaa
     4141 aagaaagaat acaattatat tgtaaaggca aaagtatcaa aaagatttat ttttaaccct
     4201 aaattagaaa tatatagtgt tgtaatagaa taatagtgtt aaaaaaattg taaaagttaa
     4261 agaagtcaaa ttctgatgtg aaaaataaaa tataccatgg aagacataaa agaaaaactg
     4321 gataactagt ttatttaatg gcagaccgtt aaggcctgcc atttttatgt attttagcgg
     4381 aataaatata atcatgcggg agtgtaaact tactgtttca aaaagtatac tttttgacat
     4441 gccttgattt tctttgttta tgtatggcat aataaccaca taatacaatt tgaaacgttt
     4501 ctgatttaat ctgattgttt tgaaacaatt ttagtgaaaa aagggtagat aaaaacgatg
     4561 agaaaatttt tttatatccg ggtcagtagt tatgatcaat cgatcgaacg acaactgatt
     4621 tccgcgaagg aattaggaat ccctgaagaa tatatatttg tagaaaaagc cagcggcaaa
     4681 gattttaaac gccctgaata tcaattaatg aaacggatgt ttcgaaaagg agatttactc
     4741 tatctacaat cattagatag actaggcaga aataaacaaa tgattttaga agaatggcga
     4801 gaattaacta aggaaaagca gattgatatt tctgtgttag acatgcctct gttagatact
     4861 gctagttaca agcaactaga tgggttagag acgcttatct ctgacttaat tctgcaatta
     4921 ctcagctata tggccgaaga cgagcggaaa cgaattaata gccggcaaag agaaggcgta
     4981 gaagctgcga aacaaaaggg tgtaaaattc ggcagaaaga aaattgcttt acctcctgaa
     5041 tttcctgcga tctattctga ctggaaagca ggaaagataa ctgcagtcta tgctatggat
     5101 caattgggca tgaaacgaaa taccttttat cgacgggtga aagaatatga acataccttg
     5161 caccactttt cttgatagat ataacagcta ttagacggga agggagagtt tgaaaatgga
     5221 cttaacgaat caaagggtcg tttttactgg aacgctgcag caaatgacac gaacccaagc
     5281 catcggtttg gttcactctt taaacggcca ttgtcaatca agtgttactt caaaaacaaa
     5341 tttcctggtt tgtggtcagt attctaagtc gttgtttgat gattctctct atacaaaaaa
     5401 ggaacaaacc gcgcgggact tgattgcgtt aggtcatgag attaccattt tatcagaagt
     5461 cgaattttat gcgttaatta gtcaacagtt gaaagaatgg caacggtatt tataatcgtt
     5521 agtaaaattt acaaggagga aaatttttat gaagcgaaaa tcttatgatc gttggggcat
     5581 tgaaataact agttttaatc gaaatattgc ttttcacgtg gagtgtgact gtggggaatt
     5641 agcaaaaagt gtttatcaaa aaaattactt ttactgtcct aaatgccatc gccgatacgg
     5701 ggtacatgga acatagaaac gaaatagtgc ggtagaaaag cgaatgaatg tagtatattt
     5761 ctacaaatca ttcgctttct ttattttata aatagttggt cgagagatcc cgttttcttt
     5821 tgcaatctct gcaactggaa tgccttgctt taacatgtcc accacggttt taaaaactaa
     5881 gcgtttttgc ggatctgttg agttaataga atactcgatt ggtcgtccct tatatcggcc
     5941 ggctttttga gccaataata ctccttgccg ctgacgttca cgaattttct cacgctcgtt
     6001 ttgtgcaata taacttagca gtgataaaaa catatcgaac atagcggtat ccaggagttc
     6061 atttccagta ttaaaatcta aaaactgtgc atctaatatt tttaacgcta cttttttctg
     6121 tttcataaaa gcaactgtat tttttatatc ttcataatct cttcctaaac gatctagtga
     6181 tgtaacgatg acgcaatcac cttcacggat ataatttagt aatttttgaa actcaggacg
     6241 ctcagtatct ttaccactgg ctttttcttg gaataattta tcaatgttaa gtccttccat
     6301 cattgccaat tgtcgatcca aattttgatc ttgtgaagat actcgaatat agccaatttt
     6361 tcccataaaa aactcctttt taaaagtgat ataatttaca tgtaaaattt acttgtagtt
     6421 ttactgctat catagcattt ttaaaaataa attttatgta gacttaaaat ttacttcatg
     6481 gagggtttaa atggatgaaa tcaaaagtaa caaaaaagca tttggcattt atttaggaga
     6541 acctagtaca gaacaattaa accaatttt
//
LOCUS       DAZKKW010000115         6381 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.117, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000115 DAZKKW010000000
VERSION     DAZKKW010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6381
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.117"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1057..1722)
                     /locus_tag="ACG8PO_002532"
     CDS             complement(1057..1722)
                     /locus_tag="ACG8PO_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0207796.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDF
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            1848..3884
                     /locus_tag="ACG8PO_002533"
     CDS             1848..3884
                     /locus_tag="ACG8PO_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HFQ0207797.1"
                     /translation="MIIKKKFTVLLTIIVTGIMSGFALVSINKQVLYRTNEFFHQTDT
                     YTPIRLPQHFIGQEEYEQLIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLE
                     MTLYYQGDVKSSHEKVPYSNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERVISLF
                     TDRFNQAFHTDYATNIFEDFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLL
                     MNKEISLLLNNDFSLHQVLWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYV
                     FLFSGIYGISLISLLLVEKIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFS
                     SSQLAVSHEPPKEIAGEDYHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSI
                     LVNVSDYLNEENEQFGRGIKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRH
                     TDLKNDLAQITEYYQEISEKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSM
                     YLERNILSGEIYPPLKIKIGSLSKERLFELIEENQLRDNLQLSFPYTQTEEIAVKVLS
                     RSFHYLIQIFLLTFFSFFLLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNL
                     IKWGTTSVIFLFLIEREPKYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            3888..4526
                     /locus_tag="ACG8PO_002534"
     CDS             3888..4526
                     /locus_tag="ACG8PO_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0207798.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            4554..4982
                     /locus_tag="ACG8PO_002535"
     CDS             4554..4982
                     /locus_tag="ACG8PO_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0207799.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
     gene            5439..6350
                     /locus_tag="ACG8PO_002536"
     CDS             5439..6350
                     /locus_tag="ACG8PO_002536"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFQ0207800.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
BASE COUNT         2206 a          971 c         1182 g         2022 t
ORIGIN      
        1 ggctaggaac ctgataacat caaggttcct agccttttta taataaaatg aagcgaaaat
       61 aatttctttt aaagagccga gattatcagc tatataagag tgaatttgat gcaaaaaaca
      121 gagccgaaac agtcttggtg ttcgtagctc tgcttatatg tttcgaattt aagacaaaga
      181 gttggcaaaa gatgttgata aggaagataa gataactgtg ttatataaac taggtaacta
      241 actgtaacaa gctaatttta agagattctt attagaagac gtttaggaat ataactaaga
      301 aaatgattat acaggtaatt gtcatttcaa taatgttttc taattttcgc gtctttgttt
      361 taagaaaaag gccagaatca gttggaaaaa agttcgtacg atagtgaaga ggggcaaaat
      421 ttcctactaa tattcccact aacaagagat aaaacggcca ggttggtgtg attcctctat
      481 aaaaaaatgc ccatgctacc gaaatcccat agattaacaa ctgaacaatt tttacaatga
      541 aaaaattgtt ctgccattac tcaaagtctc ttcctccttg tatcgtttat ttgtttcatt
      601 ttatcattat gataggcgct tgaacaatcc attttgtatt ttattgagta ctcttggata
      661 aactaccagc gattatcata tttatttatg ttactgtaag ccatcatata aaagtaaatg
      721 agcagatcat agaaatataa aaaagaacta aaattgatct aaattatgtg cagcttgact
      781 acgtaccaat ctatattact tactagattg gtcgttaatc tattcggatc gaatgagcaa
      841 aatatttttg tgaatgtcca tccttgatta tgattccaaa aagaatcatt gggaggctgt
      901 ggcgtaaata ttatactata agccagaaat acctatcatt ctgaactttt ttatttagga
      961 actttcatct caaggctttt tcaattaatt tgtacccctg ttttttggat aagatcatgt
     1021 gcattgattt tttctatctc tacgttcatc tcctacttaa tgcctatctg gctcctgagg
     1081 gcttcttttt aaggcgtcgt catagtcttg gctgaggatt tcttggtaat ctgagggaat
     1141 ggcttcccaa gtgagcaggc gaacgttcat gacgtgtttt cctttgtgtt tgattacagc
     1201 atggtttttc tctttttcta cctctccagc gtgaaaagac agccatgtac catggccaaa
     1261 gatatcatta catacccaac gttcgatgga attgtccacg tatcctacag ggagtgtggt
     1321 atagccgatt tgtggtttca aaaaaggatt gaatcgatcg atcgtttcat ttttgtattg
     1381 ataattgata agaaaaagat agaccaaaca aaagacgagc agaaagaaag atagtgtctt
     1441 tttgagtgag aagggttgtt ttgatgaata agatttcttg ttaacaagag gttcatctgg
     1501 caatgttggt tctattgatt cggtagctga ttcaatagaa ggaaaatcaa tcggcagacc
     1561 taaagtatga tgtaaaagtg acagcatttc aaagtctggc attgtacgag aattttccca
     1621 actagaaatc gttttatcgg aaacaccaat tttttcgcct aattcttttt gcgtaagctt
     1681 acaatttctg cggtaagttc gtattttttc tccggtgttc atcttcttct cctttcgatt
     1741 ctacagtata tagatttatt ctacatgacc tatacacttg atagatacgt ttttttgatg
     1801 aaaaacggta tgattttatc aaagaatcat ttaagtaagg gagagtggtg ataatcaaaa
     1861 aaaagttcac agttttactc acaatcatag tgaccggtat catgagtggt tttgccttgg
     1921 ttagtataaa taaacaggta ctctatcgga caaatgaatt tttccatcaa acagatactt
     1981 atactccgat tcgattgcca caacatttta ttgggcaaga agaatatgag cagctaatca
     2041 tggtatttga aaatgtctca tcagaaactg agctttctta tatgaaaaaa acagtaaatg
     2101 aatccaatga agtagataca tcttggaact tcaagataca gccaaagctt gaaatgacat
     2161 tgtattatca aggggatgtc aaatcttctc acgagaaggt accctattct aatacctcct
     2221 ttcgcgtaac gtccattgaa gaatcgatca aaataaaaga ttttgaaggt gaatttttct
     2281 tgatgacgag agaaaaagaa gcctatgaac gggtcatttc actattcact gatcgattca
     2341 atcaagcatt tcacactgat tatgccacta atatttttga agactttgaa aatagctatt
     2401 cgtattcggt tatttcaaat acagacgtat ataattatcg agtgtattta actattggat
     2461 tgattctttt attccttttg ttatcatcct ttttcttatt gatgaataaa gaaatcagtt
     2521 tattgttgaa taatgacttt tcattacatc aagtattgtg gtgtttattg gggaaaaaga
     2581 aaatcattat tacattcata attgcaggaa tcattggtgg ttttattttt atgtattata
     2641 aggagcaatt tctttatttg ttccagatgt acgttttctt gttcagcgga atatatggta
     2701 tctctttgat ttctctttta ttagttgaaa aaatcgcttc gttaagagaa gcgttcaact
     2761 atatccaata cattcttttt acatttggat tgttgttttc tcttcttttg accgttgcca
     2821 atgtcgaatt agcaactatc gttttttctt cttcacagtt agctgtttcc catgaaccac
     2881 caaaagaaat cgctggtgag gactatcatg tattttatcc agtaaccatc gggaaaaacc
     2941 acgtggattt tatttattcc aatcgttttg catcagcagc agatcaggaa ttatatgaaa
     3001 ctttgaataa aaacggaagt atattagtca atgtatcaga ttacttgaat gaagaaaatg
     3061 aacagtttgg cagagggata aaaataaatc tgaattatct taagaaattt cctttgattg
     3121 atgtctctgg aaggttgatc caaataaccg aaaaagaaac gcaccctgtt atattgatcc
     3181 ctgaacgata tagacataca gatttaaaaa atgacctggc gcaaataaca gaatattatc
     3241 aagaaatcag tgaaaaagaa ccgaaatttc tatttataaa aaacaatcaa cccatttata
     3301 catttattcc ttctatcccc tggatcgaac attatccggt tgttgaaata ctgacattga
     3361 aaaacagtat gtatttggaa agaaatattc tcagcggaga aatctatcca ccgttaaaga
     3421 tcaagatagg ctctttgtcg aaagaaaggt tatttgagct gattgaagaa aatcagttac
     3481 gtgataactt gcagttatca tttccgtata ctcagactga agagatagct gtcaaagtgt
     3541 taagcagatc atttcattac ttgatacaga tatttttact cactttcttt tctttcttct
     3601 tgttgagcta tgtcatgtta tgcatatatt ttgtctacaa ttgtcggaaa atcgctatct
     3661 ttcgttcaag tggttattcg ctctttgaaa cgtataaaga tttcttcatg atgaacctca
     3721 tcaaatgggg aacgacaagt gtaatcttcc tttttcttat agaaagagag cctaaatatc
     3781 tttttaatat ttttttcttt agcttcattg attttattct ttcagttgtt tttattttgt
     3841 ctacagaaaa gaaaagtcag ttattgctga tgaatggagg ttaacaattg gagccgttga
     3901 tagtgataaa aggtgcgaaa aaaatcattc gaaacaaaac gattcttcat ttggatcgtt
     3961 tcactgcgga tgctggggat cttgtgactg ttgttggaga aagtggagca ggtaaaagta
     4021 cgttattgaa tatattagga ttgaatgatc agttcagttc aggacaattc tatttgttcg
     4081 atcaagagac gaaacaatta actgggagag agaagcgctt gataaaaaga aataccatat
     4141 cttttctttt tcaagatttt gggttgattg aatatgaaac aatcgatttt aatttagata
     4201 ttggattgaa gttttcgaat ttaaatgttt cagataagcg aacagcaaag tatgaggcat
     4261 tgaaaaaagt aaatataaac aagaagctaa gtacgcctat ctacacatta tctggtggtg
     4321 agaagcaaag aatcgcctgt gcgagagtct tattgaagaa tagcaaaatc attttagccg
     4381 atgaaccgac tgggtcattg gataaaagaa ataaggataa agtagttgaa atcttatcgc
     4441 aacaagcttc ccaaggtacg ttagttatcg tagtcacgca tgatccatat ctgaccaaaa
     4501 taggaaacaa gaagatcatg ctttaaaaat caaaaaggaa aggatgtcgt tttatgaaaa
     4561 aagcagttat tatttgtgca ctagggatag gttttgttgg aggaaacgct atgtcatctg
     4621 aagtggaagc aaattcagtt cacggtgaaa tctattacaa atctttaaag ccaggagaga
     4681 ttcattttga tgctaaaggt aatattatta agtcaaattt accagtagac cgggaaattt
     4741 ctagaacagc aattaatcat agctctggaa gatgggttta ttatagtgat acaacttgta
     4801 cgagaaaaac aggtcattct aaccactata gtcgtgtcta taatcgtcac ggtgcaaaag
     4861 cgaaagttgg aaactctatt cgatcagcaa atgcaacaaa aggagtatgg gcctatgcaa
     4921 gtgcgacagg aaatagaact gacacattct catgttggta caatccaacc ggttggtatt
     4981 aagtatccaa aagtttatat aaaatagaaa aaacatataa tgaaacagcg ttaaaagtta
     5041 tttttggtag ttttaatata ttcattttta gttgtataaa aatatatcat aataaaaaca
     5101 acaaaaaaat gtctggaaaa tcgaacataa aagtgtctga tttgaatatc ccagcagcgg
     5161 aagaagaaaa acaaaatcga taacatttgt tctaagaatt agttacgtta aatacggaac
     5221 gcaagataga cttgaaaaac ggtaacaaaa gaagtagtat attaaggtgt gatgtgctac
     5281 ttgacattta ttctgctgag cgaactttct ggattgtgta gccttatatc tattcaggac
     5341 gtatcgagca gattagtaaa ggtgttcttg atgattttca tcttgaacac ctttgttttt
     5401 agcaattcgc aaaaaacgaa aagtaggaga attgataaat gaaaaaaaga tttttagcac
     5461 ttgccatcgt actaggcact ggcttattgt caggctgtac gaatgccggt gaaaaaacag
     5521 ccgttagtta taaaggcggt acaatcagcg aacaagaagt aatggatagc ttgaaaaaaa
     5581 tgcaaggagc agattccgct gtgcaacagt tgatcgttta tcaagtattt gaagataaat
     5641 atggagatga cgtatcaacc aaagaaatcg attctcaata tgatcaaaca aagaaacaat
     5701 tgggtgattc atttgattca caattgaaat ctgcaggtta cacagaacaa acattcaaag
     5761 acagtatcaa acaaagtcta gcctttcaag aaggtctaaa gaaacatatc aaattgaccg
     5821 atgaagattt aaaaacggct tgggaatctt tccatccaga agtggaagct caaattatcc
     5881 aagtagcaag tgaagatgat gcaaaagatg tgaagaaagc tgctgataag ggagacgatt
     5941 tctctaaatt agccaaagac aagtcaactg atactacgac aaaagaagat ggcggaaaag
     6001 tgaaatttga ttccactact acaacggttc cggcagaagt caaagaagca gcctttaagt
     6061 tgaaagatgg tcaagtttct gatgtgatca cttctaccaa tgcttcaact tatactacgg
     6121 aatattatgt ggttaaaatg gtgaaaaacc aaaacaaagg caacgacatg gataaataca
     6181 aaaaagaatt gaaagaaatt gcaacagaca caaaattaag tgacagtacc ttccagaata
     6241 aagtaattgg cgaagtattg aaagacgcta atgtaaaaat caaagacaaa gactttgaaa
     6301 atgttttgag tacattcact tcagattcta gtacagcaag tagtaaataa gcggtataaa
     6361 taaaaaatat gaactattta a
//
LOCUS       DAZKKW010000116         6358 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.118, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000116 DAZKKW010000000
VERSION     DAZKKW010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6358
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.118"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1411)
                     /locus_tag="ACG8PO_002537"
     CDS             complement(<1..1411)
                     /locus_tag="ACG8PO_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182-like element ISEfa7 family transposase"
                     /protein_id="HFQ0207801.1"
                     /translation="MQGVIAMMSKNNTNGRNQFAMLTIDDLVPQDHLVRKIDAALDFE
                     FIYPIVEATYSDLGRPSIDPVILIKLVFIQYLFGIRSMRQTIKEVDTNVAYRWFLGYS
                     FEEKIPHFSTFGKNYVRRFRETTVFEDIFAYILEQAVKAGFVTEDNLYLDSTHIKANA
                     NKHKFTKEMTHDEAKAYQDELEDEINRQRIEAGKRPFTLDLEKEVKLKERKISKADPE
                     SGYYVKGEREKQFAYSAHTSCDDNGFILSTIITPGNIHDSQVAFQLVKQSKRLFPEIN
                     CVVADAGYKTPKFVHFLTHLNLRPCLPYSRPKGKKGLLSKNEFLYDEYFDCYICPQDQ
                     MLAFSTVTREGYREYKSNPKECVNCPLLNQCTISKNHQRVITRHVWGDLMDEVEHLRL
                     TDLNKSIYKKRKQTIERIFADAKEKHGMRWTKYRGLEKVATHTMLVFAAMNLKKLATW
                     LWKGKEPLFFCSKIRNEVDK"
     gene            complement(1383..2582)
                     /locus_tag="ACG8PO_002538"
     CDS             complement(1383..2582)
                     /locus_tag="ACG8PO_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFQ0207802.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     NRKRDVSDIEGKVLSMYARGMSQCDIAATVEDIYGFDISHEMISDITDAVLPELEEWQ
                     ARPLAKCYAFLFVDCMYVTLRENYEAKEYAVYTILGYDLKGNKEILGLWLNQTESKNR
                     WMQVFDELKVRGVEDVFFISMDGVSGLKEGAKAIFPSVIVQRCIVHLVRNALRYLPSK
                     DYKEVCRDMKKFYSASSLNAAHAAFDSFQNRWSHYSGAVDVCKRNFSHVEQLFDYGSA
                     IRKIMYTTNAVESIHSSFRKVTKKGVFSNENAQTVDKVHFMDDVYSLFFYNRFMKTNA
                     RSDSYDV"
     gene            complement(2712..4088)
                     /locus_tag="ACG8PO_002539"
     CDS             complement(2712..4088)
                     /locus_tag="ACG8PO_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0207803.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGGVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            complement(4088..4744)
                     /locus_tag="ACG8PO_002540"
     CDS             complement(4088..4744)
                     /locus_tag="ACG8PO_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0207804.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE
                     "
     gene            complement(4754..6031)
                     /locus_tag="ACG8PO_002541"
     CDS             complement(4754..6031)
                     /locus_tag="ACG8PO_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0207805.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNNTKRNILFSSGVFTIKEGKNIEENDKNSIIVHEEFAK
                     QNNLKLGDEVDLELLDIEKSGKIKSHKFKIIGIFSGKKQETYTGLSSDFSENMVFVDY
                     STSQEILNNSENNEIANKILMYSGSAESTDLALNKLKELKIDESKYFVEKDSNAFEES
                     LESVSGIKYIIKVMTYSIMLGGMVVLSLILILWLRERIYEIGIFLSIGTSKIQIIMQF
                     IFELIFISIPSIISSLFLGNVLLKVIVDGFINSEDSMISGGSLINNSSFMSNITTLGQ
                     SYLILISIIVLSVVFASSLMLIKKPKEILSKIS"
     gene            complement(6050..6211)
                     /locus_tag="ACG8PO_002542"
     CDS             complement(6050..6211)
                     /locus_tag="ACG8PO_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFQ0207806.1"
                     /translation="MQDKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPV
                     DAISYGVRK"
BASE COUNT         1959 a         1192 c          865 g         2342 t
ORIGIN      
        1 ttttatctac ttcgtttcga atttttgaac aaaaaaataa aggctccttg cctttccata
       61 gccaggtggc taatttcttt aaattcattg cagcaaacac tagcatcgta tgcgtcgcga
      121 ctttttccaa gcctcgatac ttggtccaac gcatcccatg cttttctttt gcatccgcaa
      181 atattcgttc aatcgtttgt ttccgttttt tgtaaattga tttattcaga tctgttaggc
      241 gaagatgttc cacttcatcc atcagatctc cccaaacatg tctcgtgatg actcgttgat
      301 gatttttact tatggtacat tggtttagca gaggacagtt cacacattct tttggattgg
      361 atttgtattc tcggtaccct tctcgagtaa ccgttgaaaa agcgagcatt tgatcttgcg
      421 gacaaatata acaatcaaaa tactcgtcat agagaaactc attttttgat aatagcccct
      481 ttttcccttt gggccttgaa taaggcaaac aaggtcgaag gttcaaatga gtcaaaaagt
      541 gaacaaactt aggtgtctta tacccagcat cagcgacaac acaattgatt tcaggaaaaa
      601 gtctcttcga ttgtttgacg agctgaaagg cgacttgact gtcatgaata tttccaggag
      661 tgatgattgt gctaagaata aatccattgt cgtcgcatga agtatgggca gaataagcaa
      721 attgtttttc tcgttcgcct ttgacgtagt agccgctttc cggatcagct ttacttattt
      781 ttctttcttt cagcttcact tccttttcta agtccaaagt aaaagggcgt tttccagcct
      841 ctattcgttg acgattgatc tcatcttcta attcatcctg ataggctttc gcttcatcat
      901 gggtcatttc tttcgtaaac ttatgtttat tggcgttagc tttgatgtgg gtagaatcca
      961 gatacaaatt atcctctgtt acaaaacctg ctttaactgc ttgttctaaa atataggcaa
     1021 aaatatcctc aaacaccgtc gtttctctaa atcggcgaac ataattcttt ccaaaagttg
     1081 agaaatgggg gattttctcc tcgaaggagt agcctaaaaa ccagcggtaa gcgacattgg
     1141 tatcgacttc cttaatggtt tgtcgcatgg aacggatacc gaataaatac tgaataaaca
     1201 ccaatttgat cagaatcact ggatcgatac ttgggcgacc taaatcggag taagtggctt
     1261 cgacgattgg ataaataaat tcaaaatcaa gcgcggcatc aatctttcga acgagatgat
     1321 cttgaggaac tagatcatca atcgtaagca tcgcaaattg atttctacca ttcgtattgt
     1381 ttttagacat catagctatc actccttgca ttcgttttca taaatctatt ataaaagaaa
     1441 agactgtaga catcatccat aaaatggact ttgtctacag tctgcgcatt ctcattagag
     1501 aacactccct tttttgtgac ttttctgaag ctagagtgaa tactttcaac tgcattagta
     1561 gtgtacatga tttttctaat ggcgctgcca taatcaaaga gttgttcaac atgagaaaag
     1621 tttcgtttgc atacatctac tgcaccagaa taatgagacc accgattttg aaaactgtcg
     1681 aaggcagcgt gtgcagcgtt taaagaagaa gcactgtaga attttttcat gtctcgacag
     1741 acttccttat aatccttact tgggaggtat cgtaacgcat ttcgaacaag atgaacaata
     1801 cagcgctgca caatgacaga aggaaaaatt gcttttgcgc cttctttgag accggaaaca
     1861 ccgtccatcg aaatgaaaaa aacatcttct acaccacgta ctttcagttc atcaaatact
     1921 tgcatccagc gatttttaga ttctgtttga ttgagccata ggcctaaaat ctccttgttt
     1981 cctttaagat catagccaag aatggtgtac acagcatatt ctttagcttc atagttttcg
     2041 cgtaaagtga cgtacataca atcaacgaat agaaatgcat aacacttggc taatggccga
     2101 gcttgccatt cttccaattc aggaaggacg gcatccgtga tatcagaaat catttcgtgg
     2161 gaaatatcaa agccgtaaat atcttcaacg gttgcggcga tatcgcattg actcattcct
     2221 cgtgcataca tggaaagaac cttaccttcg atgtcggaga catctcgttt tctattagga
     2281 atcaactcag gttcaaaaga agcctcccgg tctctgggaa catcaatgtt tactttacca
     2341 aagctcgttt taagcgtctt attgccataa ccgtttcgtc gattgtcatg tttctttggc
     2401 tctttagaat gggcgtcata ccctaaatgg ttattcaatt ctccttgaag cattttttca
     2461 aataagggac caaacacatc ttttaaaaca tcttgcatat cctctacaga ttcgggttga
     2521 taagtattca aaatggattc agctaacttc tcagcatcag gatttctttt ctttctagcc
     2581 atcgtgtaat cacctcttga tcttatttta gaaaaataaa aaccgtgaag caaccacccg
     2641 cctaggattg atggttactt acacggttta tattacactc tcatttttgg tgctaaaaag
     2701 caccgaaaat tttaattatt gtcaattaat aattcttttg gattccttct taatatattg
     2761 aatgaagaaa taatcaatga tacaagaagt actaaactca taaatataac tacataaatc
     2821 atgaatttag gtaatacatt gatatctaaa cttgataatg tcttgttaaa tccttcagct
     2881 tctgccccac cacctaattg gctagatgca gattgtttag ctatttgttt agctatatca
     2941 ccagtaacct tattcaatat attattccca agcttgtcag ctgtatattg agctaagaaa
     3001 taggaaccaa cgaatgcggg gattgatata aataccatct caattaggaa ttgaccaaaa
     3061 atttctaatt ttgatatacc taatgatagt aatactgcta tttctttctt tctagcattc
     3121 atccataaga ataataataa tgaaactaca actcctgcaa atattaatga gccaaaaaat
     3181 aatttatttg aaattgagta aatacctgat atagattgtt gtaatgcagg gtaatttgat
     3241 gaacttttaa tcaaattata ttctctccaa tttatatcta atttcccaag atcttttatt
     3301 acactatcaa gattcttatc accttttaca aagaatgttg catcttggta tacagctgta
     3361 tcttcggtat taccataaac tttagcagct gtatgaatat cagtgattaa tgtattttcg
     3421 tatagttctt gtgctgcact tactccaccg ctattatgcc catcgaatag acctttaatt
     3481 tctacttcta ctgtttcatc tgcacctttc tcattatcag catcaaataa attagatttt
     3541 atttttatct tatctccaac tttaaggtta tttttttcag ctaaatcttt atgcattaag
     3601 attttatttt tatcttcatt ttctaaatgc tttccttcta ctaatttata tgcttctgat
     3661 acaaattttg tttcttttgt tgagtcgtta accccagtta acataactgt tcttttaaaa
     3721 ttctttgctc tttcaggtga ttgattcgca agagtctctt gagtttcaat aatatcataa
     3781 tcaactaaat ctgcaacact gtttattctt tttacatagg agtctatact atctgtttga
     3841 gatatctttt taatatcttc accttttaca tttcccccac ctctaggtgt tcctggattt
     3901 acttgtctat ttatctccat agaaaaacta tttgttatat tagcaaatgt ttcttttgaa
     3961 gctctgtccg tagcatcttt aatagataaa ctaatgagac ttaaagttga catagataaa
     4021 ataactaata tgataattaa tgattttaaa ctttttctag ttacataagc aaatgcattt
     4081 tttatcatta ttcaacctcc aaattcattt tatttacttt tttcaacttt ttaccactta
     4141 gttctaaaat gatatccgca gaatctgcta cttccttact atgtgttaca actattacac
     4201 atttatttct atctttagct aatgtcttta atatattaat tatttctcca gcagtaacac
     4261 tgtctagatt accagtaggc tcatcagcta gtattattgg agcatctgat accaatgctc
     4321 tagcaatagc tactctttgt tgctgtccac cagataattt cataacattt ctttttattt
     4381 gttttttatc taaacctagt tcaaacaaga tactttcatc tgctgattta tttactaatc
     4441 taatattttc aattggtgat aaataatcta ttaaattata gttttgaaat actaaagata
     4501 tattattttt tctatgatta ctatatcctt tattttgtat atcttcattc ttaaacaata
     4561 tttttcctgt ttgaggttta tcaagtccag caagtaagga aagaagtgta gattttcctg
     4621 ttcctgactt tcctactatc gcataaaact tcccaagttc aaatttttga tttactcctg
     4681 acaaaacttt ttctttagaa tttgcataac tatatgctac attctttatt tctaatatat
     4741 ccattatttt ctcctaactt atttttgata agatttcttt aggcttcttg attaacatta
     4801 atgaagaagc aaatacaact gataaaacaa taatacttat taatattaaa taactttgtc
     4861 caagtgttgt tatatttgac ataaaactac tattatttat taaacttcca ccggaaatca
     4921 ttgaatcctc tgagttaatg aatccatcta caattacttt tagtaataca tttcctaaaa
     4981 ataaggaaga tattatactt ggtatcgata tgaatattaa ctcgaatata aattgcatta
     5041 taatttgtat cttagatgtt ccaatagata agaatatacc tatttcataa attctttccc
     5101 ttaaccatag aatcaagatt aatgaaagaa caaccatccc acccaacata atagaataag
     5161 tcattacttt tattatatat tttattccac tcactgactc taaagactct tcaaacgcat
     5221 tagaatcttt ttcaacaaaa tactttgact catcaatttt aagctctttc aatttgttta
     5281 aggctaagtc tgtagattct gcactaccag aatacattaa aattttattt gcaatttcat
     5341 tattttctga attgtttaat atctcttggc ttgttgaata atctacaaac accatatttt
     5401 cactaaaatc agaagataat cctgtatatg tttcctgttt tttaccagaa aagatcccta
     5461 taattttaaa tttatgactt tttatttttc cacttttttc aatatctagt aattcaagat
     5521 caacttcatc acctaatttt agattgtttt gtttagcaaa ttcttcatga acaataattg
     5581 aattcttatc attttcttct atatttttac cttctttaat tgtaaatact ccactactaa
     5641 ataaaatatt tcttttagta ttatttgtag cttcgagtga aacaacattc ttaaattcgt
     5701 cagataaatc ttctctattt attctttgtt ctccgctaac tactttagca tcttttagtt
     5761 ttgctaatcc atcatattga attatttttt cttcaatttc ttttaatttt tcaatatctt
     5821 taaattgatt aacattaaaa tatttaccat ctttttttgt tattgatatt gaagaattag
     5881 aactttcata taaagccttt tctatttcat tgcttgattt cattattgtt aaacaagaat
     5941 acaagcaaga aagaactata gttaatatga taaaaattat taaagttcta tttttctttc
     6001 ttgtaatata tgctatagca ttttttatca ctttcctaac tactcctttc tattttctta
     6061 ctccataact aattgcatcc actggacaag catttttaca tcgtccacat ctgatacact
     6121 ccagatgatt gcaattctca actgaatctt tattcatttg gcaaactttt ttacattttc
     6181 cacaattaat acacttatcc ttatcctgaa tcggctacac tagactagac agaaaaaata
     6241 aggtgtgtag aataataaaa aacacactgg aggatttttc atgccaagac gtcaacgaag
     6301 aacctattca aaagaattca aacaacaaat cgtcgatctc tatctcgctg gtaagcct
//
LOCUS       DAZKKW010000117         6351 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.119, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000117 DAZKKW010000000
VERSION     DAZKKW010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6351
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.119"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(98..1228)
                     /locus_tag="ACG8PO_002543"
     CDS             complement(98..1228)
                     /locus_tag="ACG8PO_002543"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HFQ0207807.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     misc_binding    complement(1288..1515)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1877..3079)
                     /locus_tag="ACG8PO_002544"
     CDS             complement(1877..3079)
                     /locus_tag="ACG8PO_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0207808.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3139..3342)
                     /locus_tag="ACG8PO_002545"
     CDS             complement(3139..3342)
                     /locus_tag="ACG8PO_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFQ0207809.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3484..4548)
                     /locus_tag="ACG8PO_002546"
     CDS             complement(3484..4548)
                     /locus_tag="ACG8PO_002546"
                     /inference="COORDINATES: protein motif:HMM:NF014537.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFQ0207810.1"
                     /translation="MDSLQEEVVPVNRVLIDYLTIHFFSNQHLKLIEEIIEMPIERFE
                     ELDYAPKGYIGQYVWNQVITIRYSIDDAVKGTVMEFSGQGCKHLARWLKTRKTNWQQF
                     FRKVLDYQGNFTRIDFTLDDFVGSLSIPELKHKVILGHVWTTFQVSESHGGTDIINNE
                     SNGETLYLGSKKSQCRFCFYQKDHEQRKRRGIPLEEAKVKNRFELRYRKEKAQSLAKI
                     ISKTHDLTKLFFELLNGAICFYDRDPNDLGAKVDAKWAAFISNYGAITISLDTIPQSF
                     EKTMSWLIHGVSPSLVFIAQVDQTFHSSLLPLIIEQGEMNPRQIKLLQNMKADPEYYQ
                     EEVEYYERKLQSISNEKTSY"
     gene            complement(4609..4716)
                     /locus_tag="ACG8PO_002547"
     CDS             complement(4609..4716)
                     /locus_tag="ACG8PO_002547"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0207811.1"
                     /translation="MNQVNQHDLGEAIRVSREERGWTQRYLAEKVGISR"
     gene            4879..5691
                     /locus_tag="ACG8PO_002548"
     CDS             4879..5691
                     /locus_tag="ACG8PO_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0207812.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
     gene            complement(6065..6256)
                     /locus_tag="ACG8PO_002549"
     CDS             complement(6065..6256)
                     /locus_tag="ACG8PO_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0207813.1"
                     /translation="MKQKFFIAIIATKKFHRGKRFGQKWKFPPKVTAPQLKSIKELEF
                     ENALVICMTCTDEVFRKHF"
BASE COUNT         1897 a         1302 c         1002 g         2150 t
ORIGIN      
        1 aataaataat ataaaaggta aataaaaaag agtggatttc tccactccat gaaaagccga
       61 ctttttggga cgcttttttt tgccaagcct cacattttta tagccaaact tcttttgcga
      121 cttgctgcaa taattggatt ttcttccatt gttcttcttc tgttaaatgg tttccttctt
      181 ctgttgaagc aaacccacat tgaggactga tacttagatt ttccaatggt acaaattgtt
      241 gtgcttcttt tattcgttct tttattttct ccgtatcttc caaatctgga aacttagaag
      301 taactaatcc taaaacaact ttactttctt tgttactcca atgtcttaaa ggagtaaaat
      361 ctcctgctcg gtcagaatcg tattccaaaa agaaaccatc ataatttgtt gaaaacaact
      421 cttctgcaat gggttcatat ccaccggaaa acagccaaga agaagcgtga ttcccgcggc
      481 agatatgtgt tgtgatcgtc aaatcttctg gtttgtcttc cagtgcgtta ttgataatct
      541 ctgtacaaat atgggccatt tctttcggat caattccttt ttgaagaagt gcttctcttt
      601 tagtttgatc agctagaaac gcccaatttg tatcatcgaa ttgcaggtaa cggcaacctt
      661 tttcataaaa agcatgaatc gctgctttgt aagtagagga caagtcctca taaaaatcag
      721 atagatcagg ataaatctcc ttgatttttg aagaaccatc attagatagg atctcttgtc
      781 gtaaaatcat cgtgggacta gggatcgttg cttttgcaac aaaagtatca tctgtttcca
      841 ctacttcttt cagaaaagca aaatctttta aaaatgggtg atttggattg aaagataatt
      901 tatctactac ccgaatatta taagctggtg ctgcttttcc gtggaatgtt tgattatatc
      961 cagtttctgg aacatagcct tcgataccat ttagttcttc taaaaaatca agatgccaaa
     1021 atccacgccg aaattcacca tctgtaattc ctttgatgcc gactgctttt tggtcttcca
     1081 ctaatttttt tatttcctgg tcttcgattg cacgaagttc ttcttttgaa agtaagcctt
     1141 cttgaacttt ttttcttgct tctttgatcg attgcgggcg taagaaactt ccgacatgat
     1201 ctactgtaaa tggtctattt ttgctcattt cactcactct tttctttgtt ttttgtaaaa
     1261 aaaaatcctt ttactagcaa atactagtaa aaggacgatt atactcgtgg taccaccttt
     1321 tttcagaaaa aacattcttt ctctcagcct gacaataaaa tcagtggtct tggtaacgaa
     1381 agactgttcg tttttgccta cctttcgaca aaaaatcctc taaggccatt ttcgacagtc
     1441 aacttctagc tttttacacc aaccaaagcc tctcttgaac cattttctgt ctactttcct
     1501 tctcatcgga tactattaga ataaaattag aatagcatgt ttgaaagaaa agtcaatagg
     1561 gaataagaac aaacaaaaac taatttgtta ttcttagtaa tttgtttgtg gaatacaata
     1621 aagcttttct aatatgataa ttgttcagtc gtcctttcat cttagatcac ttccttaaaa
     1681 tgatcatacc cgtttcaacc acataaaaaa ggacttaatt ttgaaaaatt tcccatcaaa
     1741 actaagtcct ttttattata gttatgcaaa tttgcttatt ttctcgctat ttccgataca
     1801 tcttgaacag gagcaattcg actgaaaaca ctggtgcaaa agcatttatt tgtcttatgg
     1861 tagaaccgtg gaaaactcag actggtaata aattgtcatt ttttgaaaga atagcgccct
     1921 cttgaacatt atcaatcgca cttaccactt gttgatctga ctttgcatat aagtgaccgt
     1981 aagttcctaa agtgatttga atattcgcat ggcccaatcg ttcttgaata gatagcggtt
     2041 gaatattctg ctcaattaga aatgcaacat gagaatgtcg tagcgcgtga agcttaatcg
     2101 gtttgacacc cgctttttta gcagcttctt ttaaccagtt cgcaaaagaa taaggtggcg
     2161 taaatgttcc gtccaactga gcaacatatt caaaatttcc aatacgtttt tgcaagtcat
     2221 accactgtct gagttttaag gaaagcttct tccccaaccc aatcgttcga taagcacatt
     2281 tagttttggg agtcgaaaag taccaatcat cttttgtatt ataaatcaat gtcttctcaa
     2341 tcgttatcgt gtttttctca aaatctacat tcgaccaaga caaagcttca agttctccaa
     2401 tccgaatgcc agtataaaag agcatttcat acacaatctt ccgatggtgg gtttgaatct
     2461 tatcatcctt caattcattc atcacttttt gaaactcact gtgttcccaa aactcaactt
     2521 tcttattctc agaaggaagc gaagaaacat cttcaacggg atttttagca atcaccccct
     2581 ctttaacagc ccgatccaat acaattttta ggtgccctcg aatccgattg atataagatt
     2641 ttgataacgt ttcgccatta acttttttcc gtgaattacg tgactctgtc tggcttagtt
     2701 caaataacca atcctcaata tcgtgacttg taatatctcg aacacgaaaa tgatagaaaa
     2761 actgcatacg cttttcgaaa atgaattgcg cattctcata tgtttgactt ttgacctgtc
     2821 tctcgtacca ttttacaaac ctatccttgt aaaactcttc gaaagtcatt tgattggtag
     2881 aacacagttg attaggaatt gtcttgttaa aatcatgtgt ttccatgtac ttgtcataag
     2941 caattttggc atctttttgt gaagcataac ctcgtttagt cttctgaacc tttcttccat
     3001 tttcatctat acctagatac gctcggaaga accatttttt tgtcttttta tcttggtaaa
     3061 tatttttaat ttttggcatc gttccattcc tccctgtact tgagaaaagc tactttaata
     3121 gtaccgcgct tgtactgttt attccgcaac ttgaatgtga aaaagatgtt caattactcg
     3181 ggatggcacc acaccaactt ggcgattatt ataaaaatca ataccttcaa tccttgcaag
     3241 atatgccttg ctctctttaa tcattcgtgc agcttgacta ggtttaaagc ccatctctat
     3301 caaaattgca cgatttacca tgaaatcaca ttttgtttcc attctctcac ctcctgattc
     3361 tttactcctt ttaaaataat cctctttata atctattgtt tctcattcga tggttgtgtt
     3421 atactcgtct tgtcgaggcg gagtacgttg cacaacttgt tgtgtaagca gtactttgct
     3481 tttttagtag cttgttttct catttgatat actttgtaat tttcgttcat aatactcaac
     3541 ctcctcctga taatactccg ggtctgcctt catattttgg agaagtttga tctgtcttgg
     3601 attcatttct ccttgttcaa taatcaaagg caacaaggaa gaatgaaacg tttgatcgac
     3661 ctgtgcaata aaaactaatg atggtgaaac accatgaatt aaccaactca tagttttctc
     3721 aaaactttga ggaattgtat ctaaagaaat ggtgattgcg ccataattgc taataaaggc
     3781 tgcccatttg gcatcaactt ttgcacctag atcatttgga tcacggtcat aaaaacaaat
     3841 tgctccatta agtaactcaa agaacagttt ggttaaatca tgggtttttg aaataatctt
     3901 tgccaagctt tgcgctttct cttttcgata ccgtagttca aagcgattct ttacttttgc
     3961 ctcttctaaa ggaattcctc gtcgcttccg ttgttcatgg tccttctgat aaaagcaaaa
     4021 acgacactga ctcttctttg agcctaaata caaggtctca ccatttgatt cattgtttat
     4081 gatatccgta ccgccatgag attcactcac ttgaaaagtg gtccacacat ggcctagtat
     4141 taccttatgc ttaagttctg gaatgctgag actccccaca aaatcatcta aagtaaaatc
     4201 aatccgtgta aagttcccct gataatctaa taccttacga aaaaactgtt gccaatttgt
     4261 ttttcgtgtt tttagccatc ttgctaagtg tttacagcct tgacctgaaa actccatcac
     4321 agttccttta accgcatcgt caatggaata ccgaatcgtg attacttgat tccacacgta
     4381 ttggccaata tagccctttg gcgcataatc cagttcctca aaacgttcaa tgggcatttc
     4441 aataatctct tcaattagct ttaaatgttg attcgagaaa aagtgaatgg ttagataatc
     4501 aatcaaaaca cggttaactg gaacgacctc ttcttgcaaa ctatccaaca caagattcaa
     4561 tttttcttca cttaattgtc tcgttccttt ttctactttc gataataact atctcgaaat
     4621 ccccactttt tctgctaaat atcgttgtgt ccaccccctt tcttctcgag aaactcgaat
     4681 ggcttcaccg agatcatgtt gattaacttg gttcatcgac aaacctcctt ctgctattta
     4741 cttgtcaaag aacctattga acttatttta agtacatttt tattgtacgc ctgtatttat
     4801 tttaagtcaa tatgttttga ctttttttaa gtacacacgt atactaaatt taagaactta
     4861 tcctgaggag gagttttcat ggagatagac aagcaagcag tcggcaatcg aattcggcag
     4921 attcgccaag aattaaagtt gagtatggag aaatttggca aactaattgg tgatttaccg
     4981 cgaagtacag tcaataactg ggaacgtggg atcaaccttc caaaaaccga aacccttcac
     5041 caaatcgcag agataggtca tgtcaccaat gaatatcttt tgtatggtga tcaagagaat
     5101 caatacattt tagaaatgct ccaaaaaaag gcaggtaagc ttgatcctaa gattgaaggt
     5161 ctcatagtag acgaaatcaa acacgctgac cttgttagtg aaaaagaaat ggatcgaatg
     5221 attgaatttt ttattacaaa tctcattcca ccaactgaac aagaccagtt tactttccaa
     5281 tgtatcgatg aaaaaaagca gctttatctt ggttcaacta attttggtaa ggaagcccag
     5341 ctctatttgc aacatgataa tcaaaatcat attctgcaca tgatgccctt taccttctcc
     5401 aactttagtg tggatcgcat actcgtctat ttagcaaatc aaaaatccta ttcctatttt
     5461 gggaaacatt tacccaagga actaggtgag aaagccatcc ttctatactc aattaatcaa
     5521 ttgaccagcg atgtgcgaat tgccccgttg gtctattcag aagaaaccgc ttcttatcag
     5581 tacacagagg acaatcaata tctactagaa cagcaatatc tttatctacc attcgtgatg
     5641 gaagtcgaga aagaacgcct gttaaatgca gcgtatccat catcaaaatg aggtgacaaa
     5701 attgataagc acgtgacaaa gaaaatactg ctaggacact ccccaatggg tagttgtcac
     5761 agtttcctgt tctttgttag ccccctgtta gaaccggggg ctttattcct gcagtccggc
     5821 tacgccttcc tgcaatagac aaaactccag atcgggtaat tcgcttgtgg cgaaacccga
     5881 cctggagttt gtctctgtat tttgaaccct aaaaattaat caaacagact gatattctca
     5941 taatcagcgc gaaataagcc attaagtaca gttttcctac ctatttttga taacccgctg
     6001 gttagttaat attccttttc cgagagagat aaatgattgg ttaaatctat atacatctca
     6061 acctttaaaa atgttttcga aatacttcat cagtacatgt catacaaatt accagtgcat
     6121 tctcaaattc tagttcttta attgatttca gttgaggagc agttactttt ggcggaaatt
     6181 tccatttttg tccaaacctc ttcccccgat gaaatttctt tgttgcaatt attgcaatga
     6241 aaaatttttg tttcaaaaat atacataatt acttcctcca ataaaaacta tctacctctc
     6301 aaattatttt tctctaataa gaaaaacaga aaaggtaata agatacaaca g
//
LOCUS       DAZKKW010000118         6338 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.120, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000118 DAZKKW010000000
VERSION     DAZKKW010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6338
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.120"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..273)
                     /gene="prgO"
                     /locus_tag="ACG8PO_002550"
     CDS             complement(<1..273)
                     /gene="prgO"
                     /locus_tag="ACG8PO_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA segregation protein PrgO"
                     /protein_id="HFQ0207814.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(245..1198)
                     /locus_tag="ACG8PO_002551"
     CDS             complement(245..1198)
                     /locus_tag="ACG8PO_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0207815.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            1810..3303
                     /locus_tag="ACG8PO_002552"
     CDS             1810..3303
                     /locus_tag="ACG8PO_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HFQ0207816.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            3438..3734
                     /locus_tag="ACG8PO_002553"
     CDS             3438..3734
                     /locus_tag="ACG8PO_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HFQ0207817.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELRKRKKYIQ"
     gene            complement(3758..4717)
                     /locus_tag="ACG8PO_002554"
     CDS             complement(3758..4717)
                     /locus_tag="ACG8PO_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFQ0207818.1"
                     /translation="MTYTHLTTDELVMIESYFKINQSVAKTAHFLNRSRQTIHKVYLF
                     FKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIR
                     CSARTIYRMFKKGLFNPSDLPMKGKRKPNGHQERRGKQAFRRSIHEREKDYSQFSNEF
                     GHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIENKLNHWFESVPKNLFK
                     SITFDCGKEFSNWKQISNANDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNS
                     VDESFIQSVASKRNNIPRKSLNYRTPLEVFLSYVSIDDLSNLI"
     gene            4900..5751
                     /locus_tag="ACG8PO_002555"
     CDS             4900..5751
                     /locus_tag="ACG8PO_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0207819.1"
                     /translation="MKSDDWIEHTWLPLKRNSKRKKFWVLQLWPLGKLCSYGARLILD
                     LTVYSIGVFGTAYYLINDPNRIAASDIFTVLMTLIFVMLLSIFTQLVMQFTRQKLTAG
                     KLFKSLFDLIVVNAILATLIKQIEQSGRTTKGVKDFLLTFIHFVNGHMYLFEVGIILF
                     FVGYVSTKLIKRSIEKEIEIDLIPTLLGYEYNVDFGRYDELINTDIVHTSIHESTVKG
                     IRSILKNGQEMKAIDRRKEIYNFYEVEASLRYGIGKLWLDGSNIRQNKPYIFWNSSDL
                     ELPMKDK"
     gene            5772..5984
                     /locus_tag="ACG8PO_002556"
     CDS             5772..5984
                     /locus_tag="ACG8PO_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0207820.1"
                     /translation="MIPLYRIVLIVGMELKTLKKEQLFKVKERHNQLLCSKTGTMNIE
                     YRTEYLELTNQTDFLLIRKQQEQEKN"
BASE COUNT         2116 a         1016 c         1180 g         2026 t
ORIGIN      
        1 atcttttgat cgtctattat atataatccg ttcctcgtcg tttaaagtat caacataggc
       61 gtccattgca agtttgacta tttcatacat aggaatatct tttgcataac taagatcccg
      121 aatggttttt agtacgggag gatctacttg aactggtttt ctttctttcg cagtgtactt
      181 tcgttcccct aactcgacat taatatcatc tttaattgaa atatcttcag caccaatttt
      241 cttcttatct ttgttcgttg gtaataatgc catttagttt aatctccttt ccaagaggta
      301 ggatatgatt ggccattgag tcagcatatt taggtgtata atcaaatcct tcaatatcac
      361 catgttgttc atagtattca attctttctt ctaattctgt aaagatgtct gcaaaaatag
      421 cccaccatct tcgatctaca taatcattta attggatacc attctcacca tacatttcta
      481 accgatccga gcctttaaca atgcttttaa aaatgtttga ttcaccatac ttatcaattg
      541 ttttttgata gtttgcgtgt tgagaggaac gtcgcactga gaaaagaaca ggcaaaattc
      601 caatgatttg aagatgagac tcttcaaaat tcgtgacaat cggaactaaa actttattaa
      661 taaaatcttc tgttccttcc attgcgtacc gttttaattc ttgcattgca ataatataat
      721 ctgcagcagt taagaatgca cgaaccacat tatctgttga tggtgggcag tcgataaaga
      781 tataatcgaa attttttctc aattcttcta aaggtttaat gaaggccaat gatctcttac
      841 gacgatcagg atatttttcg gacaagtatt cattaagttc ttcagatcca ggagagccag
      901 ccataatata taaatgtgga cttacttgag taatacctga aacccagtcc ccttttttga
      961 ttgctgcaga catggatcga tcaaagctag taatttgata cgttgatcca atgttggaag
     1021 tagtatttct ttgttggtca taatcaatta gcaacacgtc tttttcaaaa agttgactag
     1081 aaacaatcgc taataaatca ttgtctgtag tcttaccaat gccgcctttt ctacctgtaa
     1141 gcattacaac ttttcctagt ttatctgatg ttttaaatgc agtccgtaag ttttccatag
     1201 ttccctccaa tttcctatac taaatatatt tagtatactt catatactta atatattcga
     1261 tatatctaat atactccaaa ttgataagaa ttcagacttt ataaggaata tagtggatat
     1321 actaaatata tttgctatat tcagtatatc agatatattc aaaatgtcaa aatatatttg
     1381 tgcgttttgc gttgacaatt tagagtgaaa agaggtaaac ttaattctat agaaaccaca
     1441 gataattcac tatttacaaa gtagcaaaag cagtggtact ctttaaacag aaaaattaca
     1501 taaaaaaaag agacggaaat aattgatttt agcagaatca attatttctg aatcacatgg
     1561 ttaggacatg tgttcacgct cgtctcaaga atatgtaagt agctaccaac tacttatatt
     1621 aaaagttgat cggatacctt ttccgattgc ctttattcta ccaaaaaaag gcgagcaact
     1681 caacagaaga acgtttattt agtgtactta aattaaatat tcttaaacac tgaagagaga
     1741 acacatacta tggctgagat taaagctaaa ctgtgttctc tttttgtttg agaaaggacg
     1801 ttacaatcga tgttgagcga aatttattca gctattctta aaaacggctt acgtacatac
     1861 aaatatcgaa acagcaccat gaaacctgca ggtcatattg attccagtag gagtggtgct
     1921 atctttgcgt accgtagcaa gcaattaatg aatgttgggc gtggtatggt cattacttcg
     1981 gaagaagcta ttttagagaa cgaaaagaaa ctaacgcatt ggacacctaa tacctatcgc
     2041 tttggtactt atgcggatga ttatcgaaca gtggttaaag gtcattctga gaaaaactta
     2101 agtcaaatca acacatttgt agttgatatc gattcaaaag agaaccatca aggtgaaatt
     2161 attttagcct gtttagatca agtgggctac atgccaacgc taattttaga atcagatcat
     2221 ggttatcaag tttattttgt actaaagaca ccaggctatg tcacaaagaa aagcaatttc
     2281 aaggtgatcg atgtagcaaa aaaaatttca aaaacaattc gtctacagtt ggctgaaaaa
     2341 ttgtccggag tggatctagg atgtaaccac ttcggaattg ctcgattccc acaagaaaat
     2401 aatattgttt ttaaagaaat gaactatcag tattcttttg ctgactggat tcaatggtcg
     2461 atgaagcttg aatcagatca aaaaacaaaa gaagatcgag aacgaaaact aattgtcttt
     2521 cctgagaaaa aagaaattcg tcaagtagat gaaccgtggt tcgatatgtt acttcataaa
     2581 gcaaacatcg tcggcggaga aggacgtctt ggtcgaaata atgtgatttt cacgctttct
     2641 ttagcttatt actcttcagg ttatgcattg gatacttgcg aatacaatat gttcacattc
     2701 aacgaacggt tacaggaacc gctatctgaa gcagaattag taaagattgt acgtagtgct
     2761 tattctggta attatcaagc agcaagcaga gaattcatta ttgaattgtg ccagcaatgg
     2821 gtttcaccaa ccttaaaaaa taatgatctg ttcattcaac gacgtggctg gtggaaattt
     2881 aaaaagccac gagatcaaag aaaatatagt cataaaaatg aatggaaaga agatctattg
     2941 gcttatctaa gcgaaaaaag tcttgctagt gagccttatt tgaacctgtc aaaaaaagaa
     3001 ttggcttcac aactaacgat gcccaagcga acacttgata agatcttaaa tgagttacga
     3061 gaggagaata aagtcttcta caaggtaaca cgaggaagaa atggcggaat gatcattgct
     3121 tcagtcaaaa cgttgttcgc gagggttatt caactaagta aagaaaagag actagattat
     3181 tcaacaagga tcacagaggt atttggactc aacgagagct tagtagaaga gacatttaga
     3241 caattatcaa cccccttgaa ccctcttaaa gaagtgaatc tttttgaagt agatacaggt
     3301 tgaaaaataa aaggtgcaaa ttgccttata caatatatct atgatacttc tgtaggtgtt
     3361 gctccctttg cctagctacg tgtaggtggt atacttataa ctgcgtatgc agtcttatgt
     3421 atcatggagg aagttgtatg tcgttaaaaa attatgtcta ttcacatcct gttaatgtgt
     3481 ttatcattgg tcgtttggga ttatctgtgg aagtgttctg tgagctttat ggttttaagc
     3541 aggggacttt gtcttcttgg gttaccaggg aaaaaacagt tgcttctttg ccgattgagt
     3601 ttcttcatgg gttgtcctta gcttctgggg agactatgga tcaggtatat gattgtttgt
     3661 gtgtgttaga gcaggagtat attgagtata aaatagctaa cgagttaaga aaaaggaaga
     3721 agtatatcca gtagtagttt ttatttactc ttaattgtca aattaagtta gacaaatcat
     3781 caatacttac gtaactcaaa aatacttcca aaggtgttcg ataattcagt gattttctag
     3841 gaatattatt tcgtttcgat gcgacagatt gaataaaaga ttcatctact gaattgaaat
     3901 ccatagattt caataaacca tctctacgta acaatccgtt agaattctcg tttaggcctc
     3961 tttgagacgg cgttcctgga tcagcgaaat aaatggcaat atcattggca ttactgatct
     4021 gtttccaatt tgaaaattcc tttccacaat caaaagtgat ggatttgaat agatttttcg
     4081 gtacagattc aaaccaatga tttaatttgt tttcaatatc aatcgcttgt ctaccacacg
     4141 gtttcaatgt gatgataact tttgataatc gctcaactaa ggtaattaca gcacttttat
     4201 gtttcaaacc tacgatagtg tcaccttcaa ggtgaccaaa ctcatttgag aattggctat
     4261 aatctttttc acgttcatga atagagcgac ggaaagcttg ttttccacgt ctttcttgat
     4321 gtccatttgg tttacgcttg cctttcatcg gtaagtcaga aggattaaat agcccctttt
     4381 tgaacatacg ataaatggta cgagcagaac aacgaatagg aaacgctgca cgaccaacaa
     4441 tcacatcggg tgtccatcct tgaacaacct ttctttgaat atattctgat tgttcctcag
     4501 gtaaaacaag cgggcgtcta ccacagtttg atttgttttt cttatactgt tgatagtact
     4561 ctaaggctga ttttccttgc ttgaagaaca ggtatacttt atggatcgtt tgtcttgaac
     4621 gattcaagaa atgcgcagtt ttcgcaacag attgatttat tttgaaataa gactctatca
     4681 ttacaagttc atccgttgta agatgggtat aggtcatttg tactcactcc ttataattct
     4741 ttggacggaa ctattttgag tgtagcacaa atgacttttt tagttgtcta gcttaatttt
     4801 acaatcggcg tacttaaaaa attggttcat tgagcaacct atcgctgcta aagtagcaaa
     4861 ggaaaacgtt aaaggaaaga atttagggaa gaaatcgaaa tgaaatcgga cgactggatt
     4921 gaacacacct ggcttcctct gaaaagaaat agcaaacgaa agaaattttg ggtattgcaa
     4981 ttatggccat taggaaaact ttgtagttac ggggcacgac ttattcttga tttaaccgtt
     5041 tactcgattg gagtatttgg aacggcttat tacttgataa atgatcccaa taggattgca
     5101 gcatcagaca tttttacagt gctaatgacg ttgatttttg tcatgctatt gtctattttt
     5161 actcaattag tcatgcaatt taccaggcaa aaattaacag caggaaaact tttcaaaagc
     5221 ctattcgatt taattgttgt aaatgcaatt ctagcgactt taataaaaca aattgaacaa
     5281 tctggaagaa caacaaaagg agtgaaggat tttttattga cgtttattca ctttgtaaat
     5341 ggacacatgt atttgtttga agtgggtatt attctctttt ttgtcgggta tgtatccacg
     5401 aaacttatta aacgttcaat tgaaaaagaa attgaaattg acctgattcc gacacttctt
     5461 ggttatgagt acaatgttga ttttggaaga tatgacgaat tgatcaatac ggatatcgtt
     5521 cacacgagta tccatgaatc tactgttaag ggcatcagat ctattttaaa aaatggccag
     5581 gaaatgaaag caatagatcg aagaaaagag atctacaatt tttatgaagt cgaagcaagc
     5641 ctacgatatg gcatcggaaa attgtggtta gatggaagta atattcgtca aaacaaacca
     5701 tacatttttt ggaacagtag tgatttagaa cttccaatga aagataaata attatggaga
     5761 atcgtttgaa aatgattcct ctatacagga ttgtgcttat agttggcatg gagttaaaaa
     5821 ccttaaagaa ggagcagtta ttcaaagtga aagaacgtca taatcagtta ctatgtagta
     5881 aaacgggtac gatgaacatt gaataccgaa cagaatattt agaattaacg aatcaaactg
     5941 attttttgtt gattcgaaag caacaagagc aagaaaaaaa ttaatggcta cttatagcca
     6001 attgattgat gaatgggaac aattcaagga atctaaaatt ccagaacgtc tctaaaagcc
     6061 tttatttgta aagagcataa ggaaacgtgg gaacactttt taaaaaaagt gttccaatag
     6121 gctgtgccta ttacgtttct caaaaataaa tgtgggaaca aaatatttaa aaataggata
     6181 gtgaaaggat agtgaaggat agtaaaagga gggtagtagg atagcaaaaa ggataggctc
     6241 cttttcgcta tcctttttaa attggaggaa ttaatatgga agtaagaata agaaatgtgg
     6301 atgagaaaat cgtaagagaa attgatcgaa gatgtgac
//
LOCUS       DAZKKW010000119         6041 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.121, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000119 DAZKKW010000000
VERSION     DAZKKW010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6041
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.121"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            155..1375
                     /locus_tag="ACG8PO_002557"
     CDS             155..1375
                     /locus_tag="ACG8PO_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HFQ0207821.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            1372..4092
                     /locus_tag="ACG8PO_002558"
     CDS             1372..4092
                     /locus_tag="ACG8PO_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0207822.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            4076..5020
                     /locus_tag="ACG8PO_002559"
     CDS             4076..5020
                     /locus_tag="ACG8PO_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFQ0207823.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(5089..>6041)
                     /locus_tag="ACG8PO_002560"
     CDS             complement(5089..>6041)
                     /locus_tag="ACG8PO_002560"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFQ0207824.1"
                     /translation="ACSGDTNKDIATMKGGTITVSDFYDEAKLESSNQSLVQRMIIYK
                     VFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAGYTKDTYKEYIRNNLAFEAGL
                     KAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDVKKSADDGDDFSKLAKDKSTD
                     TETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDVITTTNPTSYATEYYVVKMVK
                     NQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEELKDANVKIKDDAFENVLSAFT
                     TTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDSSK"
BASE COUNT         2113 a         1000 c         1173 g         1755 t
ORIGIN      
        1 aaaatcatgt cacaatcttt ttttcgaata aacttatttt ttgaagtcgg ccatttatcg
       61 taatttcttt gcataatacg aacaaatttt cggttattga tgaaaaaaaa cattgaaaat
      121 ggtatgataa aagaaaaaaa ggaggaaatt tttaatggtg aaatttatcc atgcggcaga
      181 tttacatatg gatcggtcat ttgaaggact gacaactctt gataaaactg tacaggaaaa
      241 actattgaag acgaatctga ccgttttatc aaatatcatt gaacaggcaa taataaataa
      301 cgttgatttt gttttacttg caggagataa ttttcatcaa aatcgtcctt cattaaaaat
      361 acagaaacat ttttcagaac agatgaaacg gttagaaaag aaccatatac ctgtatttat
      421 aatattcgga aatcatgatt tctatcaaaa agagcggtat tggttctcct ttcctaaaaa
      481 tgtccattta tttacatccg aaacggtaga gacaaaaaaa ataacgataa aaagtggaga
      541 aactgtctct ctcagtggat ttagttatcg tcaaccgtgg attcaaaaag acaaagtgat
      601 ggaatttcct tctagagaac tcacagatta ccatattggg ttgtaccacg gggaacctgg
      661 cgtctctcaa aaaggaaact atgctccttt ccaaccctca aagatgcaag aaaagggata
      721 cgattattgg gctttagggc atatccatgt gccaacggtc ttaaatgaaa agcaaacaat
      781 tgtttatcca ggtgcgcctc aaggtcatac taaaaaggaa caagcttcta catctattct
      841 actagttgaa ttaagtaaag gaagttgcca aatacatccg attaaagtag cagaggtcta
      901 ttggaagaca aaggaaatat cattgaggaa agcaagaacg acaaaagagg tcattgttca
      961 cattcgtcag cagctttcaa aagtagagga tggatttagt ttaattgaga taaaattaaa
     1021 agattatgac catctcaata cagatgtttt ggaacggata caatcaggag agctattaga
     1081 ctatttgtta gaggaattct cagataggat aaatgatttc tttatttggc gtatttctct
     1141 catagagaat actttttata cgaagacccc acttgctgct tctgctaaat tgatggaaca
     1201 gttatttcaa tactatcaga cacctcaaga tttccaacaa attttaaatg aagtatatag
     1261 tcatcaagaa gcggctagaa tattgagcga actgccggaa tatcaagaag aaacacttga
     1321 aaaagccaag cagctgctta atcaagattt tttatttgag gaggatcagg aatgaagatt
     1381 ttaaggatcg agattgctgc ttttggaaaa tggcgtcaaa aaagttttga cttttattca
     1441 ggcaatcagc tgatctatgg gggaaacgaa gcaggaaaat caacgattta tcaatttatt
     1501 caagcaatat tatttggttt cccttcaaaa ggccgtaaaa aaaaggatta tacaccaaaa
     1561 gacggctcag cctatggcgg caaaatatgg cttaaacatc ctgtatatgg tgaatttgct
     1621 gtcgagcgct acaagcagca aaatcgcgga aaatcgaagg tgtggctggg ggatcaagtg
     1681 ggaagtgatg agttactaga aaaactgatc gcacctttga cgaaagagtt attccagcag
     1741 gtatttactt tccagcaaga gcagttgatg gaactagatc atttgagaga aaaagagcta
     1801 catgatgcgc tgatttcttt aggcatcacg ggcagttctc aagtattcca aaagcggcaa
     1861 gaatattatc agaaagcaca acaattattc aagaaaaaag gccaaaaatt actaatcaat
     1921 caaaaattaa atgaatggaa agagctgcaa gaaaaaattc atcaaaaaca agaacaggaa
     1981 gcttattttg gcaagttgat ccaacaagaa caggaatacg agtcgcaaca gcagcagtta
     2041 ttggcacaat taaaagaggc gaacaaacaa ttttcattag ccagacagca agaacttaat
     2101 ttttctttat atgaagaatg gcaatctctc aaaaaaaaca gcactacagg tcttcctgat
     2161 gagcaacttc ttttagatct ggagtctttc tccaaacggt atcagcaaac aaatgagcgt
     2221 ttggaagaat tagaagcaac actggaaaag catagcggga tggatcagca gtcagccaga
     2281 tattattttt atttagagca ggagcaggaa ctaaaagatc tccagcaact tcaatttaca
     2341 gcggaacaat taaacgaaga atggcaaaga ttacaaattg agactagaga aacaaattca
     2401 gaattgaaga ttttggaaca aaggtggcat tggcagaaaa atccgttacc ggaagaattt
     2461 ttatatgaag aagaatggaa gcatcttctg gctgaagaaa gaaggattgc tgatctagaa
     2521 agccagattt ctgtgcgtct ccaaatagct aaagaacagc aagagatgat tgaagcagaa
     2581 gtgaatgact tggaagagac ctatccagca ttattacaaa atcagtcaga gatagcacca
     2641 gtctcttcat ctaaaccgta tatgctgatg gctggcggag caataatcat tgcaggtata
     2701 tttgtaccaa tgcccttgaa agtgatcctt ttactaattg gtgcaggcat gattggctat
     2761 ttcttctaca aaaggcaacc aaaaattaaa actaaaaatg atttctctga gataaaagga
     2821 atgtggcaag aaaaactagg acaacttgat ctagccttag ctcaattagc tgaagcagaa
     2881 gaaaaacaaa agagaactgg agaagaaaaa cagattcttc agcaagaagt atatcgtttt
     2941 gcggatcatc ggcacttagg aaagatggat acgctcaaac tcatgaaaga atatggacag
     3001 gaagtcatag attatcagaa attatatggc gtatttgagc ggaaacataa aagagaacag
     3061 gaaatacaag aaaaactacg acaaatcgac caaaaatttg agtttgttaa agaatggtta
     3121 ccgatacaag ataagacaat ttcagaaaaa atggtattgc tttcccgttt tatacaagag
     3181 atggaacaga tgaaatttgc cagaagctat cagcaaaata ctttattgaa gcaagaaatc
     3241 aatcaattga aaaagaaaca aaaagtatgg atacaagaat acagcgaaca attgactcaa
     3301 gcaggcttat cttatccttc ggaagtacct gtctttttac aaaaagccaa acaagccgaa
     3361 cagcagatga aaagatacaa tgaattatcg gaaatgctta ctccactatt tcctgaagag
     3421 ctgacaaaag agaaattatc tgtaagactg cgccaactga aagaaaagca atcccaactc
     3481 cagcaagaag aaaaagaagc aagtgaaaat cggcaacgac tccaacttca agtagaagag
     3541 ttgcaaagag acggcacatt ggacgaactt tatcaacaag ctagccggca aaaagcaaaa
     3601 ttacaggaat tactggagga atggggagca cttgaaatcg ccgggacatt cttaggtgat
     3661 ctttcaacag agatgagtga aagacaatta ccccagttgt taagagcagc aggtgattat
     3721 ttaggtattt taacaaaagg aaattatcag aagatattac tgaaaaatga tacactgact
     3781 ttaacagacg gatatacaga atttcccatc tatgagttat ctacaggtac gaaagatcag
     3841 ctgattatgg cgatgagatt tgcttacctc tcattagaag ctaaccgttc gatatgcccg
     3901 atcatcattg atgatggctg gcttcattat gatcatcagc gaaagtatca attagctgaa
     3961 cttttagcta aatttggaga aaagtatcaa ataatttgtt tttcttctga taaagaaatg
     4021 gtaagctatt accatgagat gaaccaaatc gtaaaacaat tagaaggagc aaataatgaa
     4081 aaaaattcgt gatttagtaa tcgatgagca gtttgaacaa tttgtcctga tcaaaagtgc
     4141 agatgtaaga atcgctaaaa acggaaaaaa atttattgct tttactttcc aagatacttc
     4201 aggtactatc gatggtaaat actgggatgc ttcggaggag gaaatcaata aatttacagc
     4261 cggcacagtc gtttttctaa acggtaaaag agaggtttat caaggaaacc ctcaagtaaa
     4321 gattcttcat atgcgcttaa caagggtaga tgagccagga gagcctagtt tatacatgga
     4381 aagagcccct ttgaagaaag aagaaatgga agaagaaatc aatcaattgt tgtttgagat
     4441 caccaatgcg cattggaatc gtattgttcg ctatctactt aataaatatc aaaaacaatt
     4501 ttttgagttt ccggcagcaa aaagaaacca tcatgcattt gccagtggat tagcttatca
     4561 tactttgaca atgcttcact tagggaaatc aatcgccgat gaatatcctg agctgaatcg
     4621 agcattgttg tatgctggta tcattttgca tgatttagga aaggtgattg aactttccgg
     4681 tcctatgtcg acagaatata cgcttgcagg aaatttgatc ggtcatctgg tattgattga
     4741 cgaagaaata actaaagcct gtcttgcgtt gaaaatcagc gaagctgacg aagatgttct
     4801 tgtattacgt catatggttt tagcacacca tggcttatta gaatatggct cacctgtacg
     4861 tccaagaata atggaagcag aaatattaca tcaaatcgac aatatcgatg cttcgatgca
     4921 gatgatgttg acttcgattc gtcaaacaga accaggaaaa tacaccgatc ggattttcgg
     4981 tatggacaac cgaagtttct atgtaccaaa agatttatag caaaaattta tttttttcat
     5041 tcaaaacaaa aaaagcgaga cataagtctt cgcttttttt gttttctttt attttgatga
     5101 atcagttgtt tcttcagttg aagattcttt tgttgaatca gttgattttg tgtcagaact
     5161 ctttgttgaa gctgtagttt cacttgagtc tttcgtactt gatgaagttg ttgtgaatgc
     5221 actcaacaca ttttcaaagg catcatcttt gatttttaca tttgcatctt ttaattcttc
     5281 accgattact ttagttgtga atgcattgtc acttagttta gtttcagtag cgatatcttt
     5341 tagttgatct ttgtatttat ccatgtcatt gcctttattt tggtttttca ccattttgac
     5401 aacgtagtat tctgtcgcat aagaagtagg atttgttgta gtaataacat cggagatctc
     5461 accgtctttc aatttgaaag ctgcttcttt tacttctgca ggaatagttg ttgtcgttga
     5521 gtcgaattta actttaccgc catcttcttt tgtctctgta tcagttgatt tgtctttggc
     5581 taatttagag aaatcatctc catcatccgc tgattttttc acatcttttg cttcatcttc
     5641 acttgataat ttgatgattt gtgcttctac ttctggatgg aaagatttcc aagcagtttt
     5701 caaatcgtca tctgtaatat ctacgtgagc tttcaagcca gcttcaaaag ctaaattgtt
     5761 acgaatatat tctttataag tatctttcgt atatccagca gcttctaatt gtgattcaaa
     5821 agtatcacct aatgatttcg cttgtttgtc gtattctgca tccacttgct tatcggttac
     5881 tttatcgcca tatttgttat tgaatacttt ataaatgatc atccgttgta ctaaggattg
     5941 gttcgatgat tcaagttttg cttcatcata gaaatcagat acagtgatcg ttccgccttt
     6001 catcgttgcg atatctttat tagtatcgcc tgaacaagct g
//
LOCUS       DAZKKW010000120         5916 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.122, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000120 DAZKKW010000000
VERSION     DAZKKW010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5916
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.122"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..434)
                     /locus_tag="ACG8PO_002561"
     CDS             complement(<1..434)
                     /locus_tag="ACG8PO_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HFQ0207825.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTV"
     gene            578..928
                     /locus_tag="ACG8PO_002562"
     CDS             578..928
                     /locus_tag="ACG8PO_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HFQ0207826.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            909..1625
                     /locus_tag="ACG8PO_002563"
     CDS             909..1625
                     /locus_tag="ACG8PO_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HFQ0207827.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAAVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            1625..3073
                     /locus_tag="ACG8PO_002564"
     CDS             1625..3073
                     /locus_tag="ACG8PO_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFQ0207828.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(3143..3652)
                     /locus_tag="ACG8PO_002565"
     CDS             complement(3143..3652)
                     /locus_tag="ACG8PO_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0207829.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(3642..4367)
                     /locus_tag="ACG8PO_002566"
     CDS             complement(3642..4367)
                     /locus_tag="ACG8PO_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0207830.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHSQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWRLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
     gene            4686..>5916
                     /locus_tag="ACG8PO_002567"
     CDS             4686..>5916
                     /locus_tag="ACG8PO_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFQ0207831.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHS
                     QRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQ
                     LYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNALHYSYSNGP
                     LECLNNHIKVLKRNAYGF"
BASE COUNT         1998 a         1034 c         1188 g         1696 t
ORIGIN      
        1 tttactgtgt tttctttaaa gcctaaagga tcaatttccg gaacatctga atgaatgaag
       61 tcgtaagcat tgatcattgt ttggctatga ccggtacacc agattttctg atcgttatag
      121 taagaaagtt catgaccgtt caactgtaca tgatcataca ttttttgaca ttcttctgaa
      181 gaaaatgttt gagaatagat ttcttcgccg tctactcgaa tgaaagaacc attcatggtg
      241 accgcagaag taatgcccgc atcttccatg atagagtgga tttctgcttc tgtacgtcct
      301 gtagcgatga cggggagaac attgttttct tttaatgcgt tcatagcttt tgccacttcg
      361 ggtgtgattt ttgatttcgc atctagtaat gttccatcaa gatcaaagaa agtgattgca
      421 cgataatttg acacaaataa aactcctttt ttattggaat aagtttatct taacaaagaa
      481 tgaacaaaag acaaaccttt cgtgaagaat tcctgaacat attggttcag tcaacaattt
      541 agagtatact ataagaagag aatttaggag ggaaagaatg atttttttca gtattggtat
      601 cttattctta ataattggca gtctttttcg gattctgccc tcaaaaggaa atctgccttt
      661 ttatgggtat cattcgcctc ttgccgcaaa aacagacgct cattggcggc tggctcaaaa
      721 gacaagcgga aactggtttt ttcttatggg cttactgatg gcattgatcg gctattattt
      781 aaagacatct gggcatacga attattttct gattgaaatg cttctgctag tatttccaat
      841 tatgcctatt tttattatga cagaaaagaa attacagaaa tatgacctgg aaactggagg
      901 gaacgataat gaatatttta atgattgaag ataatcaatc tgtttctgaa atgatgcaga
      961 tgtttttctt aaatgaaggt tgggaagcaa ctttcaagta tgatggaaaa gaagggctcg
     1021 atgccttttt agaaaaccca caacattggg atatgattac actagacctg aatttgccca
     1081 cactggacgg tatgctagta ggaagagaga tccgcaaagt ttcaagtact gtgccgatta
     1141 tcatgctgac agctagagat tctgaaagcg atcaggtgat cggcttagag atgggggcag
     1201 atgattatgt cacaaaacct ttcagtcctc tgacgcttat tgctcgaatg aaagctttgc
     1261 atcgccgtag tgagttggca gaagcaagag aagaagcagc agtttcagat gatcattttg
     1321 atatcgagac tagtcatttc aagatgaata cgaaaacacg agaagcgtat ctggacaacc
     1381 ggccaattga aggactgacg cctaaagagt tcgatctatt gtttacttta gcaaaaaaac
     1441 cacgtcaagt attctctcgg gagcagttgc ttgaattagt ctgggattat cagtactttg
     1501 gtgatgaacg gacagtggac gcccatatca agaaattacg acaaaaaatc gaaaaggtcg
     1561 gaccacaagt catccaaact gtttggggag tcggctacaa attcgatgat tctggtgttg
     1621 cctaatgcgt tatttgtatc agcaactctt agctttttgc ggaatggtcg cgttgatctt
     1681 attgatcgtt ggagtttctt ttacacaact gacaaaacaa acgattgaag aaaacaatta
     1741 ccagcaactg tttggatatg ctgaatcagt tgaaaagaca acgcaaacct atgccgatac
     1801 gctcccgcag tttagtcaag atcaggcgtt tcaaaatgca ttgctgctga cagagcaagt
     1861 actgacagaa caaaatgtga actttatatt tatcgataaa aacgaacgtg tcatttatcc
     1921 gactaataca gctttactga attttacctt aacagctgat caatggaaaa atctgaggaa
     1981 tggacgtcaa gaaaaattca catcgaataa aaatatttta ggacaagcac aggcgacctc
     2041 ttacgcactt gtgccattta atttgaatca tgaattctat ggagcacttg tagttagtca
     2101 acctgcgcga aatatcgata acagcgttcg tccaattaca ctgaacctgt tcaaaggatt
     2161 tattttttcc agtatcgttg cagttattgc cagctatgct tttgcagcat tccaagttaa
     2221 gcgtatcaat cggttaagga atgcgacgaa agaagtgacg aatggtaatt ttgatgtaca
     2281 gctccccgta catgacaaag atgaatttga tgagttagca gatgatttca ataaaatgac
     2341 gaattcattg aaagaatctc aagcagaaat tgaagaacaa gaaaatcgtc gaagacaatt
     2401 tatggcagat gcttctcacg agatgagaac acctcttacg acaatcaacg gacttctgga
     2461 agggttagaa tacaatgcaa tccctgaaaa tcagcgagaa aacgcgatta aattgatgaa
     2521 aaatgaaaca gagcgtctga tccgtttggt gaatgaaaac ttggattatg aaaaaatccg
     2581 tacgaatcaa atcagtatgg tcattaaaaa attcaatgga acagaaacat tgaaaaatat
     2641 tgttgcacag ttagaagcta aagcagaagc agcaggagat acattgacat tgaaagccgt
     2701 tgatgatatt gatgtttacg ctgattatga ccgatttgtc caaatcatgg tcaatattat
     2761 tcaaaacgcg atacagttta ctgaaaacgg ccaaatcacg attactttag aaaaaggcta
     2821 tcttgaaacg atcatcacga ttgaagatac gggaattgga atgtctgaac aacaaatgaa
     2881 aagtatctgg gatcgttatt ataaagtaga tccttcaaga aaaaatacaa aatatggtga
     2941 atcaggatta ggtttaccaa tcgtgcagca acttgtacgc ttgcataaag gaaaattaga
     3001 agtagaaagc gaactaggaa aaggaacaaa attcgctgtt cgtttgcctg acgtagaaat
     3061 caaaaaagag taaaaaacaa gcggtataaa agatcatgaa tcgatcttct ataccgcttg
     3121 tttttactat aaaatgattg atttagcccg cgggctctaa acgatcaata taaggacttt
     3181 gagcaatagt agcatcatga gtaaagagta agataccttg attttcttca tgagctaaat
     3241 aagaaagcaa gtggagtagc tcttggatct ccaatacact aagttcatca aatgtatctt
     3301 caataacaat attgtttcgc ttggaaagca aggcatgaag taattgcagc tttacttttt
     3361 caaaagacgt caactcagct gccgcacaat taagcaagga aggagagata tcaagttttt
     3421 cttgataggc caaacgatct tgtttgattt ctttttccgg tacatccaac aggacatggt
     3481 ctttaagatt cagataagga aacatcggcc aatttttttg gatgatcgca aaattatcca
     3541 gttttgcaac aagaacctct tttttcaagt gctccaatat tttatgctgt ttttcgagag
     3601 gacacaaaat aacggtcaag tgttcagcat gaatcaaata ttcaggtttc attacgtcca
     3661 ggcctcctac tttgcaaatg atggataaaa aaagtgaaag ggaatagact aagaatgaga
     3721 cagcatccac tgagcccaat aagagtttgg gcaaatccct taagtatatc tgtgaaaaaa
     3781 agatcatttt ggaaatcgat ggtaaaaaaa gtctgttgat ccatcggaat tgtaagtccg
     3841 cttaccgaag tcaagatatc acggatatct ggcagttcag tagtctttaa gacaagaaaa
     3901 ttgatccttt gaagaagcgc ttcataaact gactggaagc tgacgatcca caataaaacc
     3961 aagatacaac aaagaaagag aggaaagaac agctgccaga aaataaaaag gaataatttg
     4021 gttttagaaa gaccagtcag gcgccaattg atcaattcct gcttgttttg cctgactgaa
     4081 tagtaaaaga aaaagataaa aaggaaggca aatagaaaaa ataaactgaa tatgcctttt
     4141 atataaaatc ggctagctaa ttctaaagcc ggaagaagct ctgtagatgc agaagcgcca
     4201 ataaatgaaa gccgatcttg gacttgattg gctaaagccg attgcgaatg gtaaagagtc
     4261 aggcatgcta tcaataaaaa agcagaaaag cttgaaaata aacaatagag tattgtctgc
     4321 tttttgtaat aaattaatcg agcagtagca tagcggatta ttttcatact tttcacctct
     4381 ataaaaagta tagaaaatga atatgaataa agtatgaaat tcctttagtg aaatcacgaa
     4441 ttaaaaccag aaaatatttt gtaaaaagca agaggttgtg aagaagctgc cctattctat
     4501 taccgtagat gcaagctttt gaacactgtt tattcgaaaa gaggagcagc gacaataatt
     4561 ttgggctcta tactttttga tgttggtgaa cttcaccaac atcttttttg tataaaaaaa
     4621 tatagaaaag acaccctgaa atcctttaga attaatttgg ctaccaattc ataggaggat
     4681 tcaagatgtc tcatactcag cttatcaaag ataccctaaa tattctagac ctaaatatct
     4741 atttcgagga aaattgttta actaaagaaa aatacaaagg gcaaatttgc ttgatttaca
     4801 aaggaactct tctgtacaag ccagaagagt gttcccattg tctttgtgtc gtcccctcta
     4861 ggatcattcg ttgggggacg acaaccgttc ggttgttact gaatgatgtg tctgagtatc
     4921 gcacttatct ggagctgaag aaacaacgat ttaaatgtaa gtcttgccaa agaacattcg
     4981 tagcggatac ctctgttgct gaaaaacact gtttcattag tcaaaaagtt cgttggtctg
     5041 tcattgctcg cttaaaagag aatacttcca tgacggaaat cgctcgtcag aaaaatattt
     5101 ccacttcttc ggtctatcgt gtcatgaagc gattctaccg cccattgaat cctttcaaac
     5161 agactttgcc aaaggtctta tgctttgatg aattcaaatc ggttcgaagt gttgccagtg
     5221 caatgagttt tatcatgatg gacggacaaa gccatgcatt attggatatt gtcgaaaatc
     5281 gacgcttgcc ttatttagaa cgctattttt ctcggttttc attagcgact cgtgaagccg
     5341 ttcaattgat tgttattgat atgtatgcgc cttatgtttc gttggtgaaa aagcttttcc
     5401 cgaatgctca gttgatcatc gatcgtttcc acatcgtcca acatattggc agaactttct
     5461 tgaatcatcg agtgaaagaa accacggtac gacttaaagg aaattcccac tctcaacgag
     5521 ggttagggaa aaaattgaag cgctattgga aactcctaca aaaagaagaa gcaaagttaa
     5581 actatgaaaa acgggtttgg cgtgccagtt ttaaagatta tttgactgaa agtgagattg
     5641 ccgatcgact gttagctgct tgtcccaact tacgccaagg ctatcagtta taccaagaca
     5701 ttctttatgc ggtaaagaaa agagatcagt cactatttga ggattgttta actagagaca
     5761 ttagcggtct gccagaaacc tatacaacca ctttacgaac gtttaaaaag tttctgccac
     5821 agatccaaaa tgcgcttcac tacagctatt caaacggacc attggaatgc ttaaacaacc
     5881 acatcaaagt attaaaacgc aatgcgtacg gctttc
//
LOCUS       DAZKKW010000121         5878 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.123, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000121 DAZKKW010000000
VERSION     DAZKKW010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5878
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.123"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            642..1376
                     /locus_tag="ACG8PO_002568"
     CDS             642..1376
                     /locus_tag="ACG8PO_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0207832.1"
                     /translation="MQNTFQSKSLYYQLVEILKQRIENSMVAHDKLPSERELTSFYGV
                     SRTTVRLALQELEKMGYIYRQHGKGTFVSELRTNATDLAGAYSFTEQMRAMGKHPETK
                     ILAFKKIEATKYLASHLNVQLGEPLFQLDRLRLADDLPMMIEKSYLPVKLFMSLSQEI
                     LFLKPLYNLFSENFNQIVRLADEEFYASIVQKEDSELLNIPEGSPVLHLVRSTYNLEN
                     EVIEFTLSVARADKFHYKIRHLRSTN"
     gene            1392..2561
                     /locus_tag="ACG8PO_002569"
     CDS             1392..2561
                     /locus_tag="ACG8PO_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFQ0207833.1"
                     /translation="MFMFSKEELRKLGAENTTREIRQQPELWHETFDRYQETVADLDR
                     LFTDAKQHAGERPIRVIFTGAGTSQYVGDTLIPFLTSQGNTNQFIFQSIGSTDIVATP
                     KEYLIPDETTILVSFARSGNSPESLAAVKMANQLVNKIYHIVITCAPDGLLATQMPTK
                     KENLVLLMPNKANDAGFAMTSSFSCMTLTALLIFDQASNQQKERYVHQLSRLGEEVIQ
                     RESEIQGYLASDFHRIIYLGSGSLGGLTREAQLKVLELTAGKIATVFDTSLGFRHGPK
                     SFVNEQTLVFLFVSNDSYTQKYDVDMLEEIKNDGIAQRVVGIGQKGSLFSGDSFELVA
                     DSLLPDGYLALADIMVAQTIALLCSVKVGNTPDTPSPSGTVNRVVKGVTIYPYEK"
     gene            2577..3572
                     /gene="lacD"
                     /locus_tag="ACG8PO_002570"
     CDS             2577..3572
                     /gene="lacD"
                     /locus_tag="ACG8PO_002570"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009025 - tagatose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HFQ0207834.1"
                     /translation="MLEWSENKRQAMERLSTPEGIITALAIDQRGALKKMMSALGMEA
                     TKEKIEQFKTLISQELTPYASAILLDPEYGLPAAQKRAAASGLLLAYEKTGYDQTVPG
                     RLPDLLSDCSVLRLKELGADAVKFLLYYDYEEAEEINRQKQVFVERIGSECMAEGLPF
                     YLELLSYDAKNVDTKSLSYAKAKPRKVIEMMKEFSKTQYKVDVLKVEVPVNMSFVEGN
                     ATGEIAYTRQEAAAYFKEQSDATDLPYIFLSAGVSAALFMETLRFAKHAGARFNGVLC
                     GRATWKDAVAPFVEKGEAATLEWLTEQGTQNIRQLNEVIRETAIPWYEKVSESTC"
     gene            3651..4577
                     /locus_tag="ACG8PO_002571"
     CDS             3651..4577
                     /locus_tag="ACG8PO_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HFQ0207835.1"
                     /translation="MNKRLLDYSASDFLKAKPMELKQAILASEGRTIMAENVPSSCPY
                     MGKSVTNAELERAAGADLILFNALDLFNPQISGVPLTLKENPIIWIKKALGRAIGVNL
                     EPVDKEVLMSENRTEISSGRIVSPETLKKANELGFNFICLTGNPGTGVSNRTIEKAIT
                     LSKSIFNGLIIAGKMHGAGVDEPIMNLDIAKRFIKTGVDILLVPAPYTVPYFMESDLR
                     GIVDYIKDFNRNKSIEEKVLVLTANGTSQDSSDQETIKKIALASKACGADIQHIGDSL
                     NGICLPENIFALGQAIRGYRHQIIMLGKSNYR"
     gene            4596..5846
                     /locus_tag="ACG8PO_002572"
     CDS             4596..5846
                     /locus_tag="ACG8PO_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFQ0207836.1"
                     /translation="MIDKVQKIAAKISNQRHLLALRDSMAAVMPLIIIGSFFMLLANF
                     PIEGFTKWLSEIGVQSFLNKASDSTFGIIGLAVTYSLGHNLAKTYGVDSLSAGLLSLS
                     GYVLLTPLLDADGSIGFPTKYFGTSGLFVGIIVALVSTEIFRWFVLKNITIKMPASVP
                     PNVSRAFVAIIPGFFVVLLWFIVVVICYKLGIENVHALIADTLAKPLSLLTKTLPGII
                     LVILIQCFFWMFGIHGAQVTGPIIEPLLLQNSDVNRIAYQAGKELPNIITYEFLYNFV
                     FSGGAGCLFALAILTFFFSKSQENKTLGKLSIAPVSFQVAEPLLFGFPTILNIKMLIP
                     FVTAPVVTTLITYFSMSMGLVAKPVGATIPWTTPPIIAGFLASGGRISGAVIQVITIA
                     INVLIYYPFFKLDDNAKLKSEKND"
BASE COUNT         1968 a          955 c         1204 g         1751 t
ORIGIN      
        1 agtttgaaaa atgctatttc gaggtagaag gatattatat aagcacggtt gactaaacga
       61 agtgataata aaaaaagtta ttcaagaata agaaaaatta tgatacgaat tcagataaac
      121 tggagaatac agagaccttt aaaagggata agaaacaaca taaattcctc ttaagagaga
      181 acgaaaggac aaagccagca acttgataca cgagctagta ttagaggaat ttattctcat
      241 agactccctt ttgaatacgt ttcaacttta attttcatgt tcataaatga tttgaaacca
      301 aagcaaatgc gttgcacttt tcctccgtct tccgaaaatc gttcaattca ctggtaagta
      361 gtcatttgat agtttgaggc aaattgttga ttgatgaagg gtaggagcta aaaaaagata
      421 ccagttgaag agagaaaaag aaaaaaatag agggtgttct tgaatgaaaa aagttggaaa
      481 attatttttc atttcctttt attcctatta aagtcagtga tgtcaatcat attaacaaga
      541 ctaaaaatga aaataaaaaa aagattgttc ttagtattga ctcataaaaa ataccactac
      601 tatacttaca ttgtggttat agccagttag gagagacatc gatgcaaaat acctttcaat
      661 caaaatcact ttattatcag ctggtagaaa tattgaaaca acgaatcgaa aacagtatgg
      721 tcgctcatga caagctgcca tcagaaagag agttgaccag tttttatggc gttagtcgca
      781 cgacggtgcg actagcattg caagaactgg aaaaaatggg ctacatttat cgacaacatg
      841 gaaaaggaac ctttgtttcg gaattaagga caaatgcgac tgatctagct ggcgcatata
      901 gttttaccga acaaatgcgg gcaatgggca aacatcctga aaccaagata ttggccttca
      961 agaagattga agcaactaaa tatttagcga gtcatctgaa tgttcaacta ggtgagcctt
     1021 tatttcaatt agaccgattg cgcttagctg atgatttgcc aatgatgatc gagaaatcct
     1081 atttgccagt gaagctattt atgtcccttt ctcaagaaat tctatttctg aaacctttat
     1141 ataatttgtt ttcagagaat ttcaatcaaa tcgtacggtt ggctgacgag gaattttatg
     1201 ccagcattgt ccagaaagaa gatagtgagt tgctaaatat tccagaagga tcaccagtgc
     1261 tccatttagt tcgctcaacc tataatctcg agaacgaagt gattgaattt acattaagtg
     1321 tagcgcgggc tgataaattt cattataaga tcaggcatct gcgttccact aactgaattg
     1381 gaggaaagaa aatgtttatg ttctcaaaag aagaactacg aaagctagga gcagaaaata
     1441 ctacccgaga aattcgacag caaccggaac tgtggcatga gacgtttgac cgttatcaag
     1501 aaacagttgc agatttagat cgtttgttta cggatgcgaa acaacatgct ggagagcgac
     1561 caatccgagt catttttaca ggggccggaa cctcgcaata tgtgggagat acactaattc
     1621 cgtttcttac tagtcaagga aatacgaacc agtttatttt tcaaagtatt ggttcgaccg
     1681 atatcgttgc tacgccaaaa gaatacctga ttcctgatga gacaacgatc ttggtctcat
     1741 ttgcccgtag tggaaatagt ccagaaagtc tagccgcagt taagatggcc aatcagctgg
     1801 tcaacaagat ctatcatatc gtgatcacct gtgcaccaga cggattattg gctacacaaa
     1861 tgcctacaaa aaaagaaaat ctagttttac taatgccaaa caaagccaat gacgctggtt
     1921 ttgctatgac aagcagtttc agctgtatga cgctgactgc gctacttatt tttgatcaag
     1981 caagcaatca gcaaaaagaa agatatgttc atcaattaag ccgactagga gaagaagtca
     2041 tccaacgaga atcagagatc caagggtatc tggcatcaga ctttcatcgc atcatttact
     2101 taggttcggg aagcttaggc ggattgacta gagaagcgca attaaaagtt ctcgagttaa
     2161 ctgctggaaa aatcgcaacc gtatttgata catctttagg attcagacat ggtcccaaat
     2221 cttttgtcaa tgagcaaaca ttggttttcc tgtttgtcag caatgactca tatacgcaga
     2281 aatacgatgt agacatgctg gaagaaatca aaaatgacgg tattgcccaa cgcgttgttg
     2341 gaattggtca aaagggttca ttgtttagtg gtgattcctt tgaattagtt gcagattcgt
     2401 tactgccgga tggctatctt gctttagcag atatcatggt agcccaaacg atcgcattac
     2461 tttgttcggt gaaggttggt aatacaccag atacaccatc gccaagtgga acagtcaatc
     2521 gtgtggtcaa aggcgtaacg atttatccat atgaaaaata ggaaagagtg tgaagcatgt
     2581 tagaatggtc ggaaaataaa agacaagcaa tggaacgtct atcgacgcca gaaggcatta
     2641 ttaccgcttt ggcgatcgat cagcgaggcg cgctgaagaa aatgatgtcg gctttaggga
     2701 tggaagcgac aaaggaaaaa attgagcagt ttaaaacact aatttcccaa gaattgactc
     2761 cttatgcctc agcaatttta ttggatcctg aatacggact gcctgctgca cagaagcgtg
     2821 cagcagcgag cggtctgctg ttggcatatg aaaaaactgg ttatgatcaa accgtacccg
     2881 gccgcttgcc ggatctgctg tcggactgtt cagttctgcg attgaaagag ttaggtgcgg
     2941 atgcggtgaa atttttactg tattacgatt atgaagaagc agaggaaatc aatcggcaaa
     3001 agcaagtttt tgttgaacga attggaagcg aatgcatggc tgaaggactg ccattttatt
     3061 tagagttatt gtcatatgat gctaaaaatg tagatacgaa atcgctttcc tatgccaaag
     3121 caaaaccgcg caaagtgatc gaaatgatga aagaattctc gaaaacccag tataaagtag
     3181 atgtcttaaa ggttgaagta ccagtaaaca tgtcatttgt tgaaggaaat gcaacagggg
     3241 agatcgccta tacacgtcaa gaagctgccg cttattttaa agaacaaagc gatgcaacag
     3301 atttgccgta tatcttctta agtgctgggg tatcagcggc attattcatg gagacattgc
     3361 gttttgccaa acatgctggg gcacgattca acggtgtact ctgtggcaga gcaacttgga
     3421 aagatgcagt cgctcctttc gttgaaaaag gagaagcggc aactttagaa tggctgacag
     3481 aacaagggac acaaaacatt cgtcaattga atgaagtgat aagagaaact gccatcccat
     3541 ggtatgagaa agtaagtgaa agcacttgtt aatgtttcta atatattagt agtgataaga
     3601 gatatgtgtg attataattt atcattattg aaactgggag gatattttat atgaataaaa
     3661 gattgttaga ttattcagct tcagattttt tgaaagctaa gccgatggaa ttaaagcagg
     3721 caattttggc ttctgaaggt agaacaatta tggcagaaaa tgtgccaagt agctgtcctt
     3781 acatgggaaa atcagtgaca aatgcagaat tggagagagc tgctggagct gatttaattt
     3841 tgtttaatgc actagatctt tttaatcctc aaatttcagg tgtaccttta actctaaaag
     3901 agaatccaat aatctggatt aaaaaagctt taggaagagc tattggtgta aatttagaac
     3961 ctgtagacaa agaagttttg atgagtgaaa atcgaactga aatttcaagt ggtagaatcg
     4021 tttctcctga gactttaaaa aaggcaaatg aattaggctt taattttatt tgtttaactg
     4081 gaaatccagg aacaggagta tctaatagaa ctattgagaa agcaataaca ttatccaaat
     4141 caatttttaa tggattaatt atcgctggaa aaatgcatgg tgctggtgtt gatgaaccaa
     4201 ttatgaactt agatatagca aaaagattta ttaaaacagg tgtagatatt ttattagttc
     4261 ctgctccata cactgttccg tatttcatgg aaagtgattt gcgaggaatc gttgattata
     4321 taaaagattt taatagaaat aaatctattg aggaaaaagt tctagtacta acggctaatg
     4381 gaacaagcca agactcttct gaccaagaaa ctattaaaaa aattgcatta gcatcaaaag
     4441 catgtggagc tgatattcaa cacatagggg attctttaaa tggtatttgt cttccagaaa
     4501 atatttttgc tttgggtcaa gcaattcggg gatatcgtca tcaaattatt atgttaggaa
     4561 aatctaatta tcgttaatta aggaggaaat gaaagatgat cgacaaagtt caaaaaatag
     4621 ctgcaaaaat tagtaatcaa agacatctac tagctttaag agacagcatg gctgcagtta
     4681 tgccattaat cattattggt tcctttttta tgttattagc aaatttcccg atagaaggat
     4741 ttacaaaatg gttatctgaa attggagtac aatccttttt gaacaaagca tcagattcaa
     4801 catttggtat tattggatta gctgttacct actctttggg acacaatcta gcaaagactt
     4861 atggtgtaga ctcattatca gcaggattat tatctttgag tggatatgtt ttacttacac
     4921 ctttattgga tgcagatggt tctataggat ttccaacaaa atactttggt acctcgggtc
     4981 tttttgttgg tattattgta gcacttgtat ctacagaaat tttccggtgg tttgtactta
     5041 aaaatattac aattaaaatg ccggcaagcg ttccaccaaa tgtaagccga gctttcgttg
     5101 ctattattcc tggctttttt gtcgttctac tatggtttat tgttgttgta atttgttata
     5161 aacttggaat tgaaaatgtt catgctctta ttgcagatac actcgcaaaa ccactatctt
     5221 tactgacgaa aactttacca ggaattatat tggttatact tattcaatgt ttcttttgga
     5281 tgtttggtat tcatggtgca caggtaacgg ggccaataat tgaaccgtta ttattgcaaa
     5341 actctgatgt aaatagaatt gcttatcaag ctggaaaaga acttcctaac attataactt
     5401 atgaattctt atataacttt gttttttctg gtggtgcagg ttgtttattt gcacttgcca
     5461 ttttaacatt tttcttttca aagagtcagg aaaataaaac tttaggtaag ttatcaatcg
     5521 ctccagtaag ttttcaagta gcagagcccc ttctatttgg gtttccaact atattaaata
     5581 taaaaatgtt aattccattt gtaactgcac cagtagtaac aacattaatt acttattttt
     5641 caatgagtat gggattagtt gcaaaacctg taggagcaac aatcccatgg acaactccac
     5701 cgataattgc cggattttta gcttctggtg gacgaataag tggtgcagtg attcaagtaa
     5761 taacaattgc aataaatgtt ttgatatact atccattctt taaattagat gataatgcca
     5821 agctaaaaag cgaaaaaaat gattagaact acctatatgt ttctaactgt agttaagt
//
LOCUS       DAZKKW010000122         5707 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.124, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000122 DAZKKW010000000
VERSION     DAZKKW010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5707
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.124"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1..625)
                     /locus_tag="ACG8PO_002573"
                     /pseudo
     CDS             complement(1..625)
                     /locus_tag="ACG8PO_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            773..1914
                     /locus_tag="ACG8PO_002574"
                     /pseudo
     CDS             773..1914
                     /locus_tag="ACG8PO_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(2040..3119)
                     /locus_tag="ACG8PO_002575"
     CDS             complement(2040..3119)
                     /locus_tag="ACG8PO_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFQ0207837.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(3222..3662)
                     /locus_tag="ACG8PO_002576"
     CDS             complement(3222..3662)
                     /locus_tag="ACG8PO_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFQ0207838.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(3719..4129)
                     /locus_tag="ACG8PO_002577"
     CDS             complement(3719..4129)
                     /locus_tag="ACG8PO_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HFQ0207839.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(4309..4743)
                     /locus_tag="ACG8PO_002578"
     CDS             complement(4309..4743)
                     /locus_tag="ACG8PO_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFQ0207840.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            complement(4843..5691)
                     /locus_tag="ACG8PO_002579"
     CDS             complement(4843..5691)
                     /locus_tag="ACG8PO_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFQ0207841.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAALGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLVFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFIFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
BASE COUNT         2071 a         1067 c          953 g         1616 t
ORIGIN      
        1 ggtcaagtac ttctttttta aaaaaagtat tcatcataaa agccactact tgcggatctt
       61 catcataaaa ttctgtagca gtacttgaaa agatgttcca ataaatatct aatgctgcat
      121 attgaatttg tacctctttt ccagaaaaag actttctgcc attattagaa aaggagatag
      181 acaaaccgta ctatgctaaa aatgcattaa tctttttcat tttcgtatac aaataagagg
      241 cactatagtt tgtttgctga ctgaaactga taatcgaatt tgtctgacag ctgaacaaag
      301 aagtcaagat ctgataactg actgattcgt tgcagtatct tccaaataga cgataatagc
      361 agtaagccgg atcaattcct tcaaaattca actttaaaaa aggtttttgc ttatcaatat
      421 gcagttctgt tagattgaga cgctcaaagt cttgttctaa ttctttgatg tatagtaaaa
      481 aatttgattc agacatatgg aaatgatctg ctaaatattg tgtagtaata aagaaacgat
      541 gttcagatag gaaaagaaaa atatctaatt ttgtattgct ttttttcttc aagataaacg
      601 ttttaatcgc ttcttgagtt tgcatgaaag aggctccttt ttacttaaat tgttaaaact
      661 tgaaaatatt actgtttttt tcataaattt agacaaaaaa acagtaaaca tataggtaga
      721 atctctacta tcttatttct tgcatacaaa aaaatcaagg agtgagagag ttatgagata
      781 ttcaaccatc ttaatatcaa catttgcagc cttaagctta tcgcttacta taaatcagac
      841 agtccacaca gaggagacag aaacagccaa atggacagaa accaagtggg ccaacgtcaa
      901 tcgaattacc tttcaggatg aattttcatc cttcaattca ggagtcgcaa aattaagaaa
      961 aggacaaaca gcgaccatca aagcaaaaat tgactattcg ggagacttag cacctatact
     1021 acaatcagct gttacatttg agaatgttga tcccactttg tcgattggct atgacgagaa
     1081 cactttgact atccaacaca agcaagcgat atttaccttc accgttacat taaatcagga
     1141 cttgttaaaa ccagcgcttt ttactgtcaa ggtatcagat ggactaccga acgatcatca
     1201 tttaacgccg tatagtcagc ggcagacaat tgagcaagat tcagccatcg actcaggagg
     1261 agatcttgtt gaagaaccaa cagataaacc agaaaatgaa aataagccag aagtaccgcc
     1321 tacagaaaat ccagacggag aacagaaacc ggaaatagaa cctggagaag aacccgatac
     1381 agaaactcaa ccagagccag acaataaatc aaaacctgaa ataacccctg gagaaaaacc
     1441 agatgtaact ccagaaccag atacggattc tggaaaccaa acagtaccgg aaacaaatcc
     1501 agacacagat aatgaaactg aaaatccaga aaaaccagag gtagatccag aagaaaaacc
     1561 agatgtgact ccagaaccag atacggacgc aagagaccaa ggaataccag aaaaaataaa
     1621 caaaaaaaca attcaagagg atggaaaaaa agagagtaaa aaatcaaatc tagcaattct
     1681 aaaaataaat gaagagcagc tgaataaaaa atcacgaata tttgatagcg cccaatcagc
     1741 tgaaacctta aaaagtagca aggatacaac ctttgctgtc ctgaaacgaa aaataaacag
     1801 ctaccaaaat ccggagagtc acaaaacaaa gttatactgt ggagcggaat cattctatta
     1861 agtatagcaa caatgctttc tgcaaaaaga tttaaacaaa acagatctct ctaacatttg
     1921 aataatgtct gtgaacgcat ttttcaaata aaaagaaata tccagaaaat gaccccaaaa
     1981 taccagattg aaatctgata tttggggtcg ttcatacttt atctttcttc atatttattt
     2041 taagggatat ctgtttcttt tggaagatgg ttcgggatct ttttcacttt caatacatct
     2101 aaaaagaagg taaagatcgg aatgccgaca atcaagcccc aaacgccaaa gaaccgttcg
     2161 ctaacaagta agaccacaaa tgtataaaaa atcggcagtt ctgttttgct ggacatcaac
     2221 ttcggattca gcacataaga ctcaattaga tggacaatca aaatcgtaat caggatataa
     2281 acaacatctc ggatgcctcc ttgggaataa gctatgaaag atagaggaat acaagaaatg
     2341 ataacacctg ctactgggat cagacttaaa atgaaaatca tgatcgctaa actcagcagc
     2401 tgatgaaagc caaatacagc caaggcgatt gtagtgagca acgtatttaa aagagcgaca
     2461 acaaattgag cttctaatac caaaccaaat gtattgacaa acttatcggc aaaatagtaa
     2521 atatcttgaa agaaccaaga aaattcactt tttaaaaaca atcttgagaa tacaattgtt
     2581 tttttctttt caatcataaa aaagaaactt aagatgaatg acatagcaaa cgccactgcc
     2641 atttttccta tatcctgtaa atagccaagc gcaatcgaag cgccattttg aagctgttca
     2701 aaaaaatcgt ttcgctcaat ataagtatgg atatattgta aaacaggatc tgcatctttt
     2761 ggctgatgct cgtaaaaatt aataacggac tggatcatgt caaatgtttg atgaataagc
     2821 atcgggatat actttgtgaa tgccagatag ataaggtaaa cgatcagtgt ataaatcatg
     2881 atagctaaaa cacctgtagg gattcggaca aatcgctgaa tgatttgaac aagatgaatc
     2941 atcaaataag taaagataaa tgtgagcaaa atggttgtaa tcatacttct aactaagaaa
     3001 agaacaaaaa cgacaaataa taaaacagtg agacggcgta ttttttcatt cgccaaaaaa
     3061 cgctgataaa cggtcaaatc gacaacctcc aaataacaaa aaataaacac gttattcatt
     3121 atatgatgga atatgaaaat aagaaagagc aaaaagaaaa accgctaaat gacgttatct
     3181 tatgagttgg ttttcctcat aaaatcgttc atctagcagt ttcagccctt tttctggtct
     3241 tgatgaaaat tatctagcat ttttcgggtc agatgttgca gtgtatccaa ttcttcctgt
     3301 tcaagtggaa gataagactt gatcttttgt ggaatacctt ctgcttgttt ttgcaacgca
     3361 gcaccttgag cagtcagcgt aacatttacg attctttcat cacccgtttc acgctgacga
     3421 ttaatataat ttttttctgc taactttttt agtaagggag tcagcgtacc agaatccaga
     3481 cacaaatcat ttcctagctc tttcactgaa gtctttttct tttcccataa aactaacatc
     3541 acaagatact gggtataggt aagattcagc ggcttgagga ggggagtgta gtgtctaatg
     3601 atttctttag aggcggcata taaaggaaaa catacttgat taactaaata tagatcttcc
     3661 atttttccac ctccgctgtt ttactatagt gtatcatagc ggatttacac aaaaaagtct
     3721 attcactaac tgctttcaga gtaacttcta tatttccagc tgtagctttt gaatacggac
     3781 acactttatg cgctgcctct aataattctt gcgtttcttc cagagaaaga ccttcgatag
     3841 atccttcaat ctctacacct aagacaaagc tcgtttcact ttctgaataa agggaaactt
     3901 gtgcactgac agtagaagcc ccagagatat ttttttgctg tttgatcaat tctaatgcac
     3961 tgttaaaaca tgcactgaaa ccagctgcaa acaattgttc agggttggtt gcattttcga
     4021 cgcgtttacc aggagctgca atattcaatt cgaatgtacg atcgggatta tgcacttctc
     4081 cagagcgtcc gcctgtgttg atcattttcg tagaatacat ttttttcata aagttaccac
     4141 ctttaattat attttataca attgaatttt acacaatata attgtggaaa gcaaagaaat
     4201 ggattggcat aagataaata agagaaagca catgaaacag acgcgctttc tcgaatagca
     4261 aacatataca aaaaatatca gagacaaaaa ataatctcat cataaacatt acatatattt
     4321 ctcaaaaagt tgttttcttt cttcaaaaat tacttcacga tggacctgat gaagcatcca
     4381 taaataacca aaaggatctc tgaacattga gttagtcaca ccaaagtcca tcatttctgt
     4441 catttcttga atctctacac agccatttcg taatgcttta ttatgtgtgt cctcgatatc
     4501 agagacgatg atattgaacc agatcgtatt tggatgttca gcagtaggag cagtcaaacc
     4561 aagagacgtg ttttcatcca gcatatggaa tctcgtacca tacaaggtaa aaatgacttc
     4621 attattgcct ctagggtaat ctgttgcctc aatcagttgt atttcatcaa agattgtttg
     4681 atacagttct agtgctttca aactatcatt taatacaaaa tcgatttccg ttcctttatg
     4741 cataaagatc tcctctcctt ttcagtcgaa cagttactag attcttttat tataccagct
     4801 aaagaaaacg cattcaaaaa aatagcggat ttacttacca actcattcga ttggatgata
     4861 agtaaatccg cgtcccatta cttctgctac gctgctgaca atgacaaagg cacttggatc
     4921 gatttcttct atcaatggtt tcattcggga aaattcatga tcagtcacaa cgatcaataa
     4981 aacctctttt gactttcgat tatacccgcc ttttgcctct agcagggtaa ggccacgtcc
     5041 aatctcagca cttaagcgaa ggcggatgtc ttccaaataa tgttcactca tgaccataac
     5101 agatttcttt cgattgatac ctgtttcgat ataagtcatg acaattgaag tgattgcaat
     5161 agataaaata gcataaagaa actcttctat tccaaaaata aacagattaa gagaaacaac
     5221 gaatgcgtct gtaaaaaata agccaatcga agtattcaag tggaaatatt ttttaaaaat
     5281 gaggggaggg atcgtcgtac cgccgctaga agagttgttt ttatataaaa tcgcaactcc
     5341 taaagcaaaa atcgcacttc caaaaatgac agacaacaac cgatctgaag taatcattgt
     5401 ttctggaact attgcaatga ttaaaggaaa aaccaagctt ccgaaaagta ctttaaaaaa
     5461 gggtttttta cctaaaaata gaagagctag taccaacata acgatattca acactaaaat
     5521 aactatagct cggtcaaagc ctaaagcagc ttccagtaat attcctagac cgctgacacc
     5581 tccagcagca atatgatgag gccctaaaaa catattgata gcgactccta atatgaagag
     5641 ggcaaaaata atcttaatgt atgtccatat aaattgtctc gtttctttca tttattcact
     5701 tccttaa
//
LOCUS       DAZKKW010000123         5689 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.125, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000123 DAZKKW010000000
VERSION     DAZKKW010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5689
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.125"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(488..1297)
                     /locus_tag="ACG8PO_002580"
     CDS             complement(488..1297)
                     /locus_tag="ACG8PO_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFQ0207842.1"
                     /translation="MVKESVIGKKELRSIFWRSFFMQSSWSFDKMMAYGQMNGIERSL
                     RKIYPNDDDYYTALQRHTETFNITPHIAPFVMSLSVTMEEENAKNPEFDTDAINKIKT
                     GLMGPLSGIGDSFFWGTFRVIAAGIGISFAQQGNILGMILYFTLYTAIHFIFKWLATK
                     YGYVLGTKFIDDTEDNHMIDKLSHGASILGLTVIGAMIGTMVTLNTSLKFTVGGAETS
                     LQSIFDQIFPGLLPLGITFLCVWLYSKGHKTITIILGIFVLCFLGTLGGIF"
     gene            complement(1297..2043)
                     /locus_tag="ACG8PO_002581"
     CDS             complement(1297..2043)
                     /locus_tag="ACG8PO_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFQ0207843.1"
                     /translation="MIQAILIALVAFLASIDEYTFGASMMGRPLFTAPFVGLILGDLE
                     SGIIIGATLESMFMGSIMVGSATPPEVYASSVLGTAIAIQSGQGVGTAVALTLPLSIF
                     LQMWRNFAYAMPITYFNNKMDEALVNHDFKKAQKYHYVAQPLIVGVPSAILVFVATYF
                     GVSTINAMIKLIPQVVLDSFDVAAGVLSCVGLALLIKMMANKAMMPYLFLGFVGVMYL
                     KMDVVGVAVVGLCLALLAVQKLTFEEEEDL"
     gene            complement(2061..2528)
                     /locus_tag="ACG8PO_002582"
     CDS             complement(2061..2528)
                     /locus_tag="ACG8PO_002582"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0207844.1"
                     /translation="MIKLLRVDERFVHGQVAFAWTNNLGANCIFVASNEIATNNLRKK
                     TLKMAVPTGTKFVVKDMTEAISVLNGEKIKKYQVFLIVGTVEDAYEITQHVPEIKEVN
                     LGNLKKNENRKMITSSVYLNEQELDLVKKMTENGITVQCQAVPTDKKINPLHD"
     gene            complement(2528..2938)
                     /locus_tag="ACG8PO_002583"
     CDS             complement(2528..2938)
                     /locus_tag="ACG8PO_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFQ0207845.1"
                     /translation="MRKIVIATHSMMAKGLKDTLEFIIGPQKSLLAITAYIDSDYSIN
                     QEIEQLFSDLAPDDELIVTVDLLGGSVSNAFSEQIGKDNFYLISGVNLPLLLELCLSG
                     EQDTKKLIQKAMNSGNEGIVLVNELLEKTEEDDF"
     gene            complement(2949..3962)
                     /locus_tag="ACG8PO_002584"
     CDS             complement(2949..3962)
                     /locus_tag="ACG8PO_002584"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFQ0207846.1"
                     /translation="MLGFSEERFLSVLNGAVKNIETINQVADAISEKGFKNLYLVGSG
                     GTYATISPLAYLLSTHSELDYYHEIAAEVVCSQPKKINKDTVLITSSLTGTTQETVDV
                     VKYANSQGATTIAFVGNAATPLGEIVDYAIENDADNDTLVEELQLQFFALGARLMKNN
                     GEFPEYDEFIQTLQKMPEVLLSVRQQNDQKALEFAEKHKNTEFHMFVGAGNTYGAAYE
                     YAMCVLEEMQWIATKSIHAAEFFHGTIEMTDKSMSFVLLKGEDQTRPLVDRVERFVNK
                     YSDVVTVFDTADFELAGVPSAMRQYVAPMVMATALERVSAHFEKVRDHSLDIRRYYRT
                     VEY"
     gene            4179..4913
                     /locus_tag="ACG8PO_002585"
     CDS             4179..4913
                     /locus_tag="ACG8PO_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0207847.1"
                     /translation="MNTEELTNKLIQDIEQEKYKYAQAIPSERDFVQIYEMNRSAVKK
                     AIQQLVNKEYLLKIHGKGTFVKKNIHEHLQIKFHGLSELLEREGIQPSSLILASEIKP
                     AGHIVGKALQLEPTEEVFHLVRLRSGDGEPISIEDTYIPLNLIPDLQAFDFQIFSFYS
                     TLAAYHYQIENIYQTISSSKVSNKEARLLKTSLNRPVISLAITAVTTNEQAVEYTEVV
                     VLPEFAAIYTKNVHQGKEMKIYAQID"
     gene            4897..5607
                     /locus_tag="ACG8PO_002586"
     CDS             4897..5607
                     /locus_tag="ACG8PO_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0207848.1"
                     /translation="MPKLINLLAYDLIDKIIAYIHEHHLTPGTKLPSERDLSEMFNVN
                     RTTLRGALQKLVDNGTLRAQTNSGYYLTAPKFEKNAHHFFTPIEDSLLSKQRLQLKAT
                     ALSPKKAAALCPELNDFKQQQPQAYLEYIDAEAISLMLLIPMETLKKSNFSDNPFQDF
                     KSLAYTRSQEISLITDNDVAADLLGVDKEEVLLFVREEIRAGKELAAIALGYTISRRC
                     NIQVNVATTLQSGSVAKF"
BASE COUNT         1832 a         1195 c          943 g         1719 t
ORIGIN      
        1 aagcgacaaa aaccacgagc aggtccattc atcctcaaac ctcggtgatc ttcgcggttt
       61 aacaccatca gaagaaatgc acaaaaataa taaggagagt ctgagacata aataaattcc
      121 cagactttta gtgagtctaa atccgaataa acggtgggtc gaaagcaact ctagctgcgc
      181 gtcgatcgca gcaatctcta ggtgctatgt tcggataaat ggtggtcaaa agtcagattt
      241 tcggccattt cgtaacagcc aaccagacaa aaagcgtctg ttttgtcttc tactccaatt
      301 gctcaaatct tagcgacttt tggcttggtc ttactctaaa cagtttgtaa gcctaaactt
      361 tatttgctgt ttgtttctaa gatcaaagca aaactacttt ttagttggtt acttttacaa
      421 gttcttttta ctgttgaata aatgtaaatc ttaagaacaa atatttactt ctataattcc
      481 aagtttctta aaaaattccg ccaagtgtac ctaaaaagca taaaacaaaa attcctagaa
      541 taattgtgat cgttttatgc cctttggagt aaagccagac gcataaaaag gtaattccta
      601 atggcagcaa gcccggaaaa atttgatcaa aaatactttg taatgaagtt tcagctccac
      661 caaccgtgaa ttttagtgac gtatttaaag taaccatcgt gcctatcatc gcgccaatta
      721 ccgttaaacc taaaatcgaa gcaccatgag ataatttgtc aatcatatgg ttgtcttctg
      781 tatcatcaat aaatttagtt cctaaaacat agccatattt tgtcgccaac catttaaaaa
      841 taaaatgaat ggcagtatac aaagtaaaat aaaggatcat gcctaaaata ttcccttgtt
      901 gagcaaaaga aatacctatg ccagccgcaa taacccgaaa agttccccag aaaaatgaat
      961 cgccaatacc tgataaaggt cccattaatc ctgttttgat tttattaatt gcgtctgtat
     1021 caaattcagg atttttagca ttttcttctt ccatggtgac ggacaagctc ataacaaaag
     1081 gcgcaatatg cggtgtaatg ttaaatgttt ccgtatggcg ctgcaacgct gtataataat
     1141 catcatcgtt cggatatatt ttacgtaagg aacgttcaat accattcatt tgtccatatg
     1201 ccatcatttt atcgaaggac caagaggatt gcataaaaaa ggagcgccaa aatatacttc
     1261 ttaattcttt tttcccaata acactttctt ttaccattat aaatcttcct cctcttcaaa
     1321 agttagcttt tgaacagcca gtaacgctaa gcaaagtccg acgacagcca caccgacaac
     1381 atccattttc agatacatta cgccgacaaa tcctaagaaa agataaggca tcatcgcttt
     1441 attcgccatc attttaatta ataacgctag cccaacacaa gaaagcacac cggccgcaac
     1501 atcaaagctg tccaatacaa cctgaggaat caacttgatc atcgcattga ttgtagacac
     1561 accaaaataa gtagctacaa aaactaaaat ggccgatgga accccaacga ttaaaggttg
     1621 agccacataa tgatattttt gtgctttttt gaaatcatga ttgactaatg cctcatccat
     1681 tttgttgtta aaatacgtaa ttggcattgc ataggcgaag ttacgccaca tctgaagaaa
     1741 gatggacaaa ggtaaagtca acgcaacggc agttcctact ccttgacccg attgaatcgc
     1801 aattgctgta cctaaaacac tggaagcata aacttcaggg ggcgtcgcac tccctaccat
     1861 aattgagccc atgaacatgg attccaaggt ggcaccaata ataattcctg actctaaatc
     1921 tcccaaaatc aaaccgacaa aaggcgctgt aaatagcggt ctacccatca tcgatgcacc
     1981 aaatgtgtat tcatcaatag acgccaaaaa agctaccagt gcgattaata ttgcttgtat
     2041 catattcttc ccctcctctt ttagtcatgc aaaggattga tttttttgtc cgttggtaca
     2101 gcctgacatt gaactgttat cccgttttca gtcatttttt tcaccaagtc cagttcttgt
     2161 tcattcaagt aaacggaact agtaatcatt ttccgattct cgtttttctt taaatttcct
     2221 aaattcactt ctttaatctc aggtacgtgc tgagtaattt cgtaggcatc ctccaccgtc
     2281 ccgacaatta aaaatacttg gtatttcttg attttttctc catttaaaac agagatggcc
     2341 tctgtcatat ccttcaccac aaattttgtc cctgtaggaa cagccatttt tagtgttttt
     2401 ttgcgcagat tattggtagc gatttcatta cttgcaacga aaatacaatt tgcacctaag
     2461 ttatttgtcc aggcaaaagc aacttgccca tgaacaaaac gttcatctac tcgtaataat
     2521 ttaatcatta aaaatcatcc tcttctgttt tctctagcaa ctcgttcacc aaaactatcc
     2581 cttcattccc gctattcata gctttttgga ttagtttttt cgtgtcctgt tcacctgata
     2641 agcaaagctc taaaagcagc ggcagattta caccgctgat taaataaaaa ttgtctttac
     2701 cgatttgttc ggagaaagcg ttagacacgc ttcctcccag taaatctacc gtcacaatta
     2761 attcgtcatc aggcgctaag tcggaaaata gctgctctat ttcctggtta atggagtagt
     2821 cactatctat atacgcagtt attgctaaaa gtgatttttg aggaccaata ataaattcta
     2881 aagtatcctt taagccttta gccatcatgg aatgcgtcgc aattacaatt ttcctcatag
     2941 ctcttacctt aatattccac agtccgataa taacgacgaa tatctagtga atgatcccgc
     3001 accttttcaa aatgagcact cactcgttct aaggccgttg ccataaccat tggcgctaca
     3061 tactgacgca tagcgcttgg tacacctgct aattcgaaat ccgcagtgtc aaaaaccgtc
     3121 acaacatcgc tatatttatt tacaaaacgc tctacgcgat cgactagcgg acgcgtttga
     3181 tcttcacctt ttaataacac aaaactcata gatttatcag tcatttcaat agtcccatgg
     3241 aaaaattcgg ctgcatggat cgacttggtg gcaatccatt gcatttcttc cagtacgcac
     3301 atagcatatt cataggcagc cccataagta ttcccggctc caacaaacat atgaaattcc
     3361 gtatttttat gtttttctgc aaattcaagt gctttctgat cattttgttg ccgaacactt
     3421 agtaatactt ccggcatttt ttgtaatgtt tgaataaatt catcatattc aggaaactca
     3481 ccgttatttt tcatcaggcg tgcacccaaa gcaaaaaact gtaactgaag ttcttctact
     3541 aatgtatcgt tgtcagcatc attttcaatc gcataatcca cgatttcccc gagtggtgta
     3601 gcagcatttc ctacaaacgc aatcgtcgtt gcaccttgac tattggcata cttaacaaca
     3661 tcaacggttt cttgagtcgt accggttaat gaagatgtga ttaacactgt atctttatta
     3721 atctttttcg gctgtgaaca aaccacctct gcggctattt catgataata atcaagctct
     3781 gaatgagtgc ttaacagata agctagcggg ctgattgtgg catatgttcc accagagcct
     3841 accaaataaa gatttttaaa tcccttctca ctgattgcat ctgccacctg attgattgtc
     3901 tcgatatttt ttactgcacc atttaatact gatagaaaac gttcttcgct aaaacctaac
     3961 atagttaaaa acctccaagt caataatttg aaaaatatat ttgcaaatgc cggcacatta
     4021 acaaaccact taaccttttt tatataccac ctgttaacgt ttttaatgta ccacttatag
     4081 aaaacgctgt caatagtttt taaccacttt tatttttatt tgtaccaaaa tccttttttc
     4141 atgtataata aacaaaaata gactctggag tgtaacgtat gaatactgaa gaactaacga
     4201 acaaattaat acaagacatt gaacaagaaa aatataaata cgcgcaagct attccttcag
     4261 aacgtgattt tgttcaaatt tatgaaatga atcgttcagc cgtaaaaaaa gccattcaac
     4321 aattagtaaa taaagagtac ctactaaaaa tacatggcaa aggaactttt gttaaaaaaa
     4381 atatccatga acatttgcaa atcaaatttc atggtttgtc tgaattgttg gaacgggaag
     4441 ggattcagcc tagcagctta attttagctt cagaaataaa accggctgga cacattgtcg
     4501 ggaaagcttt acagctggaa ccaactgaag aagtatttca tttggttcgt ttaagaagcg
     4561 gcgacggtga gccaatttct attgaagaca cttatattcc attaaatctc atacctgatc
     4621 ttcaagcttt tgatttccaa attttttcat tttattccac attggctgct tatcattatc
     4681 aaattgaaaa tatctatcag acaatttcca gctcaaaagt gagcaataaa gaagcccgtt
     4741 tgttaaaaac cagcttaaat cgtccggtaa tcagccttgc tatcactgcg gttacgacaa
     4801 atgaacaagc ggttgaatac actgaagtag tggttttgcc tgaattcgcc gccatctata
     4861 ctaaaaatgt tcatcaagga aaggagatga aaatctatgc ccaaattgat taatctgttg
     4921 gcttatgacc tgattgacaa aattattgct tatattcatg aacatcattt aacccccgga
     4981 acaaaactgc cttcggaacg ggacctatca gaaatgttta atgttaatcg aactacctta
     5041 agaggcgcgt tacaaaaact ggtggacaac ggcaccttac gggcgcaaac caatagcggc
     5101 tattatttaa ctgcgccaaa attcgaaaaa aatgcccatc atttctttac accgatagaa
     5161 gattctctgc ttagtaagca gcggctacag ctaaaggcaa cggctctttc accgaaaaaa
     5221 gcagcagctc tttgtccgga actgaatgat ttcaaacagc agcaaccaca agcttattta
     5281 gaatatattg atgcagaagc aattagcttg atgttattaa tccctatgga aacattaaag
     5341 aaaagtaatt tctcggacaa tcctttccaa gattttaaaa gtttggcata tacgcgttct
     5401 caagaaattt ctttaataac tgacaatgac gttgcggcgg acttgctggg agttgacaaa
     5461 gaagaggttc tgttatttgt acgagaggaa ataagagccg gtaaggagtt ggcggcgatt
     5521 gctttaggtt atactattag tcgccgctgc aacatacagg ttaatgttgc tacgaccttg
     5581 caatccggtt ctgttgcaaa gttttaaatc tactatcaga taaggtagaa taatagaaaa
     5641 agatagcagg aggaatgacg atgagtcatt ttaaaggaaa gcaatttca
//
LOCUS       DAZKKW010000124         5651 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.126, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000124 DAZKKW010000000
VERSION     DAZKKW010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5651
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.126"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            251..676
                     /locus_tag="ACG8PO_002587"
     CDS             251..676
                     /locus_tag="ACG8PO_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFQ0207849.1"
                     /translation="MHLHRISALAVQIGRYHHSETRAVMEGLEEAIQTTNDCPYRRTF
                     HSDQGWAYQMKAYRNKLKEYKIFQSLSRKGNCLDNSLMENFFGLLKQEIFHGKVYNCF
                     EELKSAIDSYIYYYNNERIKQKLNCQSPVQFRKTTVTVV"
     gene            complement(981..1943)
                     /locus_tag="ACG8PO_002588"
     CDS             complement(981..1943)
                     /locus_tag="ACG8PO_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004233175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0207850.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGESI"
     gene            complement(1945..3384)
                     /locus_tag="ACG8PO_002589"
     CDS             complement(1945..3384)
                     /locus_tag="ACG8PO_002589"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004564 - beta-fructofuranosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HFQ0207851.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            3569..5515
                     /locus_tag="ACG8PO_002590"
     CDS             3569..5515
                     /locus_tag="ACG8PO_002590"
                     /EC_number="2.7.1.211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-specific PTS transporter subunit IIBC"
                     /protein_id="HFQ0207852.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKGTFKIDGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFVFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
BASE COUNT         1805 a          974 c          941 g         1931 t
ORIGIN      
        1 ttataaataa ctcataaaaa atattttgac tttcaaaatc acttttgcta ccagaagttg
       61 aggcgaagtg ttttaagcat agttgtaccc tagtttgacc gttaaaaata aataacgatc
      121 gctagagtgc aactatgttt gtttgctcta ccgtcagcgt cggcttggcc tctcacatcc
      181 attaccgtcg ggatcatctc cgttacatac ccttacaaaa tacgccctgt aagcattgca
      241 tgaaaccacc gtgcatttgc accgtatttc cgccctagcc gttcaaatcg gtcgctacca
      301 ccattcagaa acacgtgcgg taatggaagg tttggaggaa gctatccaga ccactaacga
      361 ttgtccctat cgtcgtacgt ttcactcaga tcaaggttgg gcgtatcaaa tgaaagctta
      421 tagaaacaaa ttgaaggagt acaaaatctt tcaaagcctg tctcggaaag ggaactgctt
      481 agataattca cttatggaga atttttttgg cttattaaaa caagagattt ttcacgggaa
      541 agtttataac tgtttcgagg aattaaaatc agccatcgat agctatatct attactacaa
      601 taatgaacgg ataaaacaga aattaaactg tcaaagtccc gtccaatttc gtaaaacaac
      661 cgttacggtt gtgtaaaaat atcattttag agcatgaaaa aaggagtgga tttctccact
      721 ccaaaaaagt ctaacttttg ggggtcacta catttttgat cctttttatt tgattaatta
      781 tatccagctg gataataatt ttgggggcaa catttctatc ttactattat cttcctaaag
      841 tagtagactt tcttattggg aaaaattaca acaaaaagga gtggcttagt tgacaactcc
      901 tttttgttta caaatcaatt ggatatctac aaattgtatt ttctaacgga ttttatagac
      961 attaaaaaat taatttgacg ttaaatactt tctccagaaa gtattgagat tggtaaaata
     1021 taatccttat ttgtcttttc gcctttaatt tttttcagaa gaatatcaac aataagactt
     1081 gcaatttcat caattggttg tcttattgtt gtcaattgtg ggaaaaattt ctcaatgaaa
     1141 gaggtaccat cgtatccaat aatttttaaa tcttctggga tattaagttt aagttgatga
     1201 gcaattttca tagtgagaat ggctgttaaa tcatcagaaa caaaaatacc atctggtttg
     1261 tgatttataa tagttgactt tatttccatt tcacgacgga tagtagacag attattagga
     1321 attttgaaaa tttttccatt tggattttga aaagaaaaac ctagtgcacg taattctgtg
     1381 ggagaatccg tgttatcatt tcctgtaatc ataatggtat tttgacaacc atttttttgt
     1441 aaagtcttag ctgcaagttt tcccccttca aagttatctg aagaaacaat ggaaacatta
     1501 ggagctaaat ttctatcaaa agcaacaata ggtgctctca cacgctcata atcagcaatt
     1561 ccaagattat ggcttgatga gattatgcca tcaacttggt tggcttcaag catttcaatg
     1621 tattctctct ctttagcagg atcattttga ctattgcaaa taatcgtttt atatccatgt
     1681 ttaaataact cgatttcgag atactcaatg agttcagagt aaaagatatt actaatatta
     1741 ggaaaaataa gaccaataag ctgaggtgac tttccttgaa gtccacgtgc taaattattt
     1801 ggtttataac caagagtttt catagcttca tgaacttttt gttttgtttt ttctgataaa
     1861 tatcccttat tattaatgac gcgtgaaacg gttgttgggc taactccagc taattcagcg
     1921 acatcagtta attttgctac cattttaata ttttaattca aagtattttc cagtaggttc
     1981 gcctgatttg atttgaatac cattttggtg attttcaggg aatacacgac tagtaaacac
     2041 tttttctcct ttattaataa aaatctcaat gattgagtta tctacaaaaa tgttagcagt
     2101 tgtttcttta gtatcaattg agcattcacg aatcgtacca aagtcagtgg catattgaat
     2161 gcctgctttg ctgcgatcta aaataatttt tccttctttg gtatctacaa tcaagctaag
     2221 tccatttcct ttactatcag agaaaagaag taactccgat ttctgatttg gaggaaaagt
     2281 tagttcaagc tcataagtat tatttgtttc tgcttttgct gtaaattgtt cagaactagc
     2341 acgtaatgat gtaatagctt ctactggata ttggtaaagt ttaccatctt ttattgaaag
     2401 ttcttttaca agactcattg ctccctgata atcatactta tcagttggat aatcgatatc
     2461 aggtagtcca atccaactga caatcagagc acgaccatca ggagtattga atccttgagt
     2521 cgcatacgct tcaaatccat aatcaagatt ttgaatttct gaagcgccca caagttttac
     2581 tttctctatg tcaagcgatt gacagatctt aaaagtattc ggatagatat tcccataatt
     2641 tagctcagat ttatccaacc cttgaggaca ataaatgaga acaggttttt tgtctacaaa
     2701 tactagattt ggacactcaa tcatgtattc tgaaccagtg ccgccaaaat caaggttgcc
     2761 aacttcctcc catgtttcca cgttattgtc atccgctttg taaagtttga taaagcctga
     2821 tttttctaaa ttttgtgctc caacaatcgc atataattgt ccttcataat taaacatttg
     2881 tggatcacga aaatgatcag ttacatcagc aggtttatga ataagaacat tctcaccttt
     2941 cgtaatgttg tagtctttat ccatccatgc tccgatttga agtgtatcac gttcccaatt
     3001 ttcatttcga acgtttcctg tatagagtaa gaatagattc ccctctattt catatgcaga
     3061 accggaataa gcgccgtggc tatctcgttt tgtgtctgga agaagttctg ctcctgttac
     3121 atgaaaatga actaaatcat ctgattcatt gtgtacccat tctttcaacc catgagcagc
     3181 tccaaatggc caattttgat aaaaaagcgt atattttccg ttaaaaaaag aaaaaccatt
     3241 aggatcgttt aacaatcctg ttttaggttc gatatgatag ctagcacgcc aaggtgatcg
     3301 ttttacatta tccttaattt tatccatctc atctttagtc caatcagaat aagcacgata
     3361 tcgtatttct cgaggtaagt tcatatttta ctccttcaat ttatcttttt acaactagta
     3421 tggtaaacgt tttccgttga aaaatcaatc atttaattta aaaaaataaa atatatgaca
     3481 aacgtttgac atatttttaa gatatgctaa aataaatttg catttcagtg aaccgttttc
     3541 acgtaaaaat aaaaaaggag tgattcgaat gaattacagt aaagtagctt ccgacgtaat
     3601 acaagcggtc ggaaaagata accttattgc agctgcacac tgcgctacac gtcttcgtct
     3661 cgtactaaaa gatgacacga aagtgaatca aaaagcttta gacgaaaatc cagatgtgaa
     3721 aggcacattt aaaattgatg gacaatatca agtgattatc ggtgcaggtg atgttaattt
     3781 cgtttatgac gaacttatca aaaaaactgg actttcggaa atttcaacag atgatttaaa
     3841 acaaattgct agtaaagata aaaaattcaa ccccattatg gcattaatta aattattatc
     3901 agatattttt gttccaatta tacctgccct tgttgcaggt ggtttattaa tggcattaca
     3961 aaacttttta acagctgctg gactttttgg accaaaatca attgaagaaa tgtatcctgc
     4021 gattgaaggt atttcctcaa tgatccaact gatgtccgct gcaccattca tgttcttacc
     4081 aattttagta ggtatttcag ctgctaaacg ttttggagca aaccaatttc ttggtgctgc
     4141 aattggtatg atcatgacaa caccagatct tggtggctca actgaatatt ggaatatttt
     4201 tggttatcat gtttcacaaa caaactatgc ttatcaagtt attccagttt tagcagctgt
     4261 ttggatatta tctatattag aaaaattctt tcataaaaaa ttgccatcat ctattgactt
     4321 tacattcacg ccattattat cagtaatgat tactggattt ttgactttca cagttattgg
     4381 ccctgtaatg ttattgttgt ctaatggaat tacagacatt attgtttggc tatataacac
     4441 tactggattt attggaatgg gtatttttgg aggaacatat tcacttatag tcatgactgg
     4501 tttacatcaa tcatttccag ctattgaaac tcaattactt tcagcgtgga caaatggtat
     4561 cggacatgga gactttgtct ttgtagttgc atctatggca aacgttgctc aaggtgctgc
     4621 aacatttgcc atttggttct taactaaaaa ttctaagaca aaaagtcttg cttcctctgc
     4681 gggtgtctca gcacttcttg gaattacaga gcctgccttg ttcggagtta acttaaaata
     4741 ccgctttcca ttcttttgtg ctttaattgg ttcaggaatt gctacagcag tagcaggatt
     4801 acttaaagta gttgctgttt cacttggttc agcaggtttc ttgggatttc tttcaattaa
     4861 tgcaacatct attccatttt acgtattgtg tgaattgatt agctttgtag ttgcttttgc
     4921 aattacttac ttctacggta aaacaaaagc tgctggtatc tttaaagcag aagaaattgc
     4981 tgaacaaaca ccagttaatt cttcaaaagt agcaacaaca gatccaatat ctactgatgt
     5041 tgaatcaaca caaacaaaag tttcatcaga aacaattgtt agtcccattt ctggtaaagt
     5101 gattagtctt tcttcagtaa atgatccagt cttttcatca gaagctatgg gaaaagggat
     5161 tgccattaaa ccaaatggaa acacggtcca ttctccagta gatggtacta ttcaactcac
     5221 gtttgaaact ggacatgctt atggtattaa atcaaataat ggtgctgaag ttctaattca
     5281 tattggtatt gatactgtat caatgggagg aaaaggattt acacaaaaag ttactactaa
     5341 tcaaaaagtt agaaaaggcg atattcttgg agcatttgac cgttcaatga ttgagaaaga
     5401 aggtcttgat gatacaacaa tgattattgt gaccaatact tcggattatt cagaagtaaa
     5461 tatagtagct gaaaatacag tcactgaggg tactgattta cttgaaatca aataaatata
     5521 tttccctgag taaagatgga attttctttc caaaatcaaa taatatagat taatcaaata
     5581 tttgagaata acattttatt taatcttttc atatatcaat tgtatgaaaa attattagaa
     5641 ttttaaaaaa t
//
LOCUS       DAZKKW010000125         5599 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.127, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000125 DAZKKW010000000
VERSION     DAZKKW010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5599
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.127"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            109..1101
                     /locus_tag="ACG8PO_002591"
     CDS             109..1101
                     /locus_tag="ACG8PO_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0207853.1"
                     /translation="MVTIKDIAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNLAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            1161..2582
                     /locus_tag="ACG8PO_002592"
     CDS             1161..2582
                     /locus_tag="ACG8PO_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HFQ0207854.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSFPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            2575..4074
                     /locus_tag="ACG8PO_002593"
     CDS             2575..4074
                     /locus_tag="ACG8PO_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HFQ0207855.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            4113..5507
                     /gene="uxaC"
                     /locus_tag="ACG8PO_002594"
     CDS             4113..5507
                     /gene="uxaC"
                     /locus_tag="ACG8PO_002594"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFQ0207856.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYLTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
BASE COUNT         1829 a         1038 c         1157 g         1575 t
ORIGIN      
        1 tttttaacgt gtgcaatctt ttcatatcaa agatctattg acttatcgta gttatcgtaa
       61 tatgatgaat gcgttaacaa aaagtgaaaa agtaaggaaa atgatggaat ggtaacgatc
      121 aaagatatcg ctaaagcagc tggcgtttct catacgacag tttctcgtgc tttgaataac
      181 agcagtttaa taaaaaatga aacaaaacaa aaaatacgac aactagctaa ggaaatgaat
      241 tattcgccaa actatagtgc aaaaggttta gtgaatcaaa aaaaatatct aatcggacta
      301 tttttctcaa gtttgcataa aggaacttca tcaaatttcc tagcagattc cattgcaggc
      361 atccattcag tattagacac aaattacagt ttatctgttg aaagtatcga tgttgttgat
      421 ttaacagaaa tcaacctgca acgctacgat ggactgatcg ttatgagtca aagtgatgat
      481 gatcagcctt tcatcgacta tctcaaagaa aaagaatttc ctttcgttgt tgtcaatcgt
      541 cacatcaatg atccagcggt tatcaacgtc actgccgatg atgcggccgg tgttatgcag
      601 gcagtagatt acggtattgg atgcggacat acgaagatcg cttatattgg cggaaagaaa
      661 aattttcgtt cgacaaaaga acgggaaaaa ggggtattga aaagtctaaa aaaagcgagc
      721 atccctgtca atctagccta ttttttaaca ggagattaca gcttagaaag cggcctccaa
      781 aacatggaat acctattatc actttctgac ctgcctactc tggttttttg cggcaacgac
      841 gacatagcga ttggtgcatt gagagctgta catttagctg gcctccgtgt tccccagaac
      901 atttctctga tcgggttcga tgattctcca gtcgtctcct atctcaatcc gccgcttacc
      961 accgtacgta agcctttgaa agaaatgtgt caagaaggaa caaagctttt actagatttg
     1021 atcgaaggca ggacgatcga acaatctaaa attgagctct ccaccttgtt gatccttcgt
     1081 gagtcgatca attacttgta aactacaagt aattttatat aaaaatgtta acgtgtgcat
     1141 aacacagaaa ggaagtttat atgaaaaaac tgacaaaaga attagtgaaa aaaaacgaag
     1201 cacctgtaaa agttcttcag tttggtgaag gcaattttat gcgtggattt attgattggc
     1261 agattcagca gctgaataac caacaattgt ttaaaggtaa tgttgcaatc attcaacctt
     1321 tagaacaagg tcttggaaat atgatgaacc agcaagataa tttatatacg gtcattttac
     1381 aaggactcag caatggcgaa atcgttgata cttccgagat tatcaccgta gtagaacaaa
     1441 tgatcaatcc ttatgatgaa tgggatgctt atctatctct agcagaaaat gacgacttgg
     1501 cctatatcgt ctccaataca acagaggcag gaatccaatt cgtggagact gataatttaa
     1561 cagatactcc cccacagagc ttcccgggca aactgacagc ctttttatac cgacgtttca
     1621 aattaaacaa agctggcttt accatcattc cttgcgaact gattgacaga aacggcgaac
     1681 gtctaaaaga aatcatttta cgctacgcag aaaaatggga tttagaacct gcttttgttg
     1741 agtggttaga aaaagaaaat gttttctgtt gtagtttagt tgatcgcatc gtcccaggtt
     1801 atcctcgcga agatgcaggt gttttcaatc aacggcataa ctatgaagat caattgatgg
     1861 tcaaagcaga accattcatg ctttgggtga tcgaagggcc taaagaattg gagaatcaac
     1921 taccattcaa acaggctggc ttgaatgtca tcatcacaga tgatatgact ccttatcgtg
     1981 aacgtaaggt gcatttgctt aatggaccgc atacagcgat ggtccctctt gctttgttgg
     2041 cggaattgga aactgtagaa gaagtaatga aagatccttt attctccgca tatgttgatc
     2101 agttattcaa tctagaattg attcctatgc tttctttacc taaagatgaa ttagccattt
     2161 atgctgacca aatcaaagaa cgtttcttga atccttttgc ccatcacaaa ttagaagcga
     2221 tttcattaaa tagtgtttca aagttcagca cccgtctgct tccagtgttc aagaagtata
     2281 tcgaagaaca aaatcaggta ccaccgttaa tcactgtttc actagctgcg ctacttctta
     2341 tgtatcgagg cgatcaagta aagccacatg atgatgaaaa gacgataagc gaatttactg
     2401 atgcttggtc tgataatgga acagctattc ctcgattatt gcaaaacgct gcgctttggg
     2461 gagaagattt gtctcaaata ccgaatgtga cagatacagt tcaagaaata gctgcaaaga
     2521 tcgatcaaaa aggtgtccgg tcagtattac aagaaatgaa aggaacgatg gtccatgctt
     2581 gaaaccctgc aaccaacaat gaaattaaat cgacgagaca gtgtggtcgt cgcattaacc
     2641 cctattccaa aacaaacaca actgacgatt gatcagcaaa cgatcaccac tcttgaagat
     2701 atcccgcaag gtcataagat tgcattgggt gatttgaaag ctggagacaa cgtgatcaaa
     2761 tatggctatc caatcggtca tgtgacgaca gacgtaactg ccggtcaatg gctgcacact
     2821 cataatgtaa aaaccaatct ttctggtgaa ttggattatc gatatgaaaa agatgtccat
     2881 ccaagtcatt acctttttga gaaccgaaca tttcaaggtt acttgcgaaa aaatggaaaa
     2941 gtagggattc gtaatgattt gtttttagta cctaccgttg ggtgtgtgaa tggcattgct
     3001 gaactgatcg tcaaacagtt caaggaaaaa catccagatc tgggacagtt tgaccatatt
     3061 acgattttaa agcatcctta tggctgttcg cagcttggaa aagatcatca aaatactcga
     3121 gaaattttag cggacgcagt aaatcatccc aatgccggag gagttttagt atttggatta
     3181 ggttgtgaga acaatacagt tcctgaattc aaaaaaattt taggtgaata tgatgaagaa
     3241 cgagtgaaat ttctagtggc tcaagatgtt tacgatgaga tcgaacaagg tgtggctcta
     3301 ttagaagagc ttttaacagc ggcggaaaat gaccagcgca tatcagtccc gctctctaag
     3361 ttgaatgtcg gtttgaaatg cggcggttct gatggtctat cgggaataac tgctaatcct
     3421 ttacttggtg cattttctga ctacttgatt gctcaaggag gcagcacgat cttaacagaa
     3481 gtgccagaaa tgtttggcgc agaacaagta ttgatggcac gagcggaaaa tgaagaagtt
     3541 tttgactcaa tcgttgactt gatcaatgac ttcaaacagt atttcttgtc ttacggggaa
     3601 ccagtttacg aaaacccatc tcctggtaat aaagcaggtg ggatcactac attagaagat
     3661 aagtctttag gctgcacgca aaaatcagga agctctcctg tcgttgatgt gctacaatat
     3721 ggcgaaaaaa tcagaaaacc agggcttagt ttattgcaag ctcccgggaa tgatcttgta
     3781 gctgcctctg ctcttgcttc atccgattgc cagttggtct tgtttaccac aggaagaggg
     3841 actccgtttg gctcatatgt cccaacggtc aaggtatcta cgaatacaac tctttttgaa
     3901 cgcaaaggtc attggatgga ttttaatgca ggcgtcttat tagaacaacc aatggaagta
     3961 atcttagaat cgtttgttca aaaaatcatc gctgtagcta gtggagaaga aacgaaaaat
     4021 gaacaaaatg acgttcgaga aattgccatt tttaaaaacg gagtaacact ttgattttca
     4081 taaaacagta taaaaagaaa gaaggaagat ttatgttttt atctgatgat tttttattaa
     4141 aagacgattg ggcgaaaaag ctttaccatt cctacgcaaa aaacatgccc attattgatt
     4201 atcactgtca cttagatcca aaagaaattt acgaaaacaa caatttcagc aatctgacag
     4261 aagcttggtt aggaggcgat cattacaaat ggcgcttgat gcgagcttgc ggcgtgcctg
     4321 aagaaaaaat cacaggaaat gcttctgatt ttgaaaaatt tttagcttgg tgtcaaaccg
     4381 taccaaaaat tatcggaaat ccgctttaca gttggtcaca tcttgaattg aagcgtttct
     4441 tcggtattga cttgttgata aatgaagaaa atgcagagaa aatttggaca caagcaaatg
     4501 aaattttggc agctcctgat tttcgccgtc gggaaatcgc aaaaaaatcg aatgtagaag
     4561 tgatttgtac aacagatgat cccgcagacg acttgcacta tcatcagttg cttgctcatg
     4621 aagaaccaga tttgaaggtt ctaccttcat tccgtccaga taaagcactc aatattgaaa
     4681 aagaaggatt taatgcatgg gtcacactac tggaagaagc agctgaccaa acgatcacca
     4741 cctatcaaga actgatagaa gtcttagggc agcggataga atacttccat caacatggat
     4801 gtcgtgtttc cgaccacgct ttagacatct tacgctataa agaagcggat gaaacagtat
     4861 tagaagagat ttttcaaaaa gcacgagcta acaaactatt atcttcagat gaaatcgatg
     4921 cttatcttac agaaacactg atacatttga cccacttcta tcattctcat aattggacga
     4981 tgcagctgca catccatgct tatcgtaact gtaatacaca aatgttggaa aaattagggc
     5041 ccgataccgg ctatgatgga atgaatgatc tttctttgac tattcctctg caaaaattat
     5101 tgaaccgtgc agaagaaacg gatcagttgc caaagacgat cctttattca ttgaatccga
     5161 atgactatcc tgcgatttta gctttgatgg gctgtttcca aaaagaacag aatggaaaac
     5221 tgcagttagg ttctggatgg tggtacaacg atactcgagc tggtatgcgc gatcaattga
     5281 cacaatttgc tgatggtagt gctttgggga attttgtcgg catgctgaca gattcaagaa
     5341 gtttcctgtc ctacactaga catgaatatt tccgtcgtgt tctttgtgaa tttttaggcg
     5401 aaatggtcat gcgaggagaa gcacctgaag atgaacaact tttaggaagt ctagttcaag
     5461 cgattagtta tacaaatgct aaaaattact ttggcttttt tgcctaattg attaatagta
     5521 acagtaacga atcaaaaaca ggatagtgaa ataacggaat ccgtttatcc acgttttaga
     5581 gaagaagacg gaccaaatt
//
LOCUS       DAZKKW010000126         5595 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.128, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000126 DAZKKW010000000
VERSION     DAZKKW010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5595
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.128"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            6..863
                     /locus_tag="ACG8PO_002595"
     CDS             6..863
                     /locus_tag="ACG8PO_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFQ0207857.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQRRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLMQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            885..1394
                     /locus_tag="ACG8PO_002596"
     CDS             885..1394
                     /locus_tag="ACG8PO_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFQ0207858.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            1431..1832
                     /locus_tag="ACG8PO_002597"
     CDS             1431..1832
                     /locus_tag="ACG8PO_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0207859.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            1944..2297
                     /locus_tag="ACG8PO_002598"
     CDS             1944..2297
                     /locus_tag="ACG8PO_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0207860.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            2331..2774
                     /locus_tag="ACG8PO_002599"
     CDS             2331..2774
                     /locus_tag="ACG8PO_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFQ0207861.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            2791..3876
                     /gene="uxuA"
                     /locus_tag="ACG8PO_002600"
     CDS             2791..3876
                     /gene="uxuA"
                     /locus_tag="ACG8PO_002600"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFQ0207862.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            3869..4582
                     /locus_tag="ACG8PO_002601"
     CDS             3869..4582
                     /locus_tag="ACG8PO_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFQ0207863.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            complement(4789..4938)
                     /locus_tag="ACG8PO_002602"
     CDS             complement(4789..4938)
                     /locus_tag="ACG8PO_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0207864.1"
                     /translation="MTFYNKDIQYRVTLDTDLNLFVVFDKENTNHVATGVTIEQAVQE
                     LKKSA"
     gene            5334..5534
                     /locus_tag="ACG8PO_002603"
     CDS             5334..5534
                     /locus_tag="ACG8PO_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFQ0207865.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
BASE COUNT         1940 a          856 c         1217 g         1582 t
ORIGIN      
        1 gggaaatggc tgaaataatg gataatcaaa caggtgctcc tgaagaaatc acgaaaaaag
       61 atgtaacaaa agcctatctg agatggcatt ttgcaaacga aattcctcac tcttttgaac
      121 gttacttggc gccatcacta ctatacggaa tgatgccaat cttgaaaaaa ctttataaag
      181 atgaagactc tttaaaagca gcatataaac ggcagttact gttcttcaac actcaattaa
      241 gctggggtgg cggtgtgatt actggcctaa tgtcttctat ggaacagcga agagcagaag
      301 aagagtatga agaaaaagag atcttgatgc aggatgactt gatgtataac acgaaagcgg
      361 gattgatggg ggcactagca ggaatcggag atgcaatcga ttcaggaacc gtccaatata
      421 tttttatcgc aatcgcggta ccttgggcac aacagggaag tcctttaggc gcactttttc
      481 cttttatctg ctttgggttg tatcaggttt tactaggggt atttttcgca agacaagctt
      541 tcactatggg ccgtaatgct acaggcctaa tgcaaaacac tggtgttcag acagcgattg
      601 aaatgctatc agttttaggg ttgtttatga tgggggtgct tgcgggaaac tatgtaaaag
      661 tagaatcttc attgaaattt gcgatatccg gacgtgagtt tgttatccag gaaattcttg
      721 atcaaatcgt gccagggata ttacctttag ctgtcgtaat gggtgtgtac ttgttctata
      781 cgaaaaaagg attgaaagtc acacaagcat tattatggct gactggaata ttaattgtgc
      841 tagcaggaat cggtatttta taaaaagaag ggaacgtgaa taagatgggt gtagtcaatt
      901 tagcgcgtgt ggatgagcgt ttgatccatg ggcaagtaat ggtaacgtta tctcaaaaaa
      961 gtggagtgaa ttccattttt gttgtagacg aagttgtagc aaaagataaa tttatgagag
     1021 atctttacaa aaatgcagga agccgtacag gacaaaaaac aattgtcatt acaccagaga
     1081 aagctaaatt ctattgggat gaatatcaat ttaaagaata caattgtatt ttgatcgcaa
     1141 aaacagtcca aacgatttac gacttagtaa aacacggaat ccccatggaa gaattgaata
     1201 ttggagggat tgctcaaaaa aatcctgata cagatctttt agtgacgaaa tctgtttatt
     1261 taaataaatc agatgctgaa aaattgaaag aaatgaatga acaatatggc gtaaagaata
     1321 tttattttca agccactcca tctgcaccta aaacaggatt aaaagatgta ttgaacaagt
     1381 ttgatttgtc ataaacaaat gcttagaaaa aagataaaga gggcgaaaaa atgaagaaga
     1441 acgagctgtt tcgtgattgg gaatttcgtt atcgctatat ttaccgtaag cgccgtacaa
     1501 aaaagagtaa acagcgtttt ctttcagcat tagtatcaga tatttacagt atgagaacag
     1561 atgtgacagt cattgcttac gatacgctag cctaccgatc aaaaaatatt tatgttggag
     1621 atatcgaaaa agcagagaaa gttatctgta cttattacga tacacccgtt catgcattag
     1681 gaagttattt catgtttgat tggaaagacc aaaggaaaaa aacaatctac tctatattat
     1741 tgtccttcat tttgttgttc agtttaggtt ggtggggaat gatgatatat aacaaaaatc
     1801 ctcatcacgt attcgatctg ctatccgtat aaacaggtat cactgtacta gcatttggga
     1861 gctacttttt cttattggga aaagcagctc gcggatggcc tagcagacaa acttttattc
     1921 gaaatacttc atccatatta acgatgctag aaatgatccg tacgattgat gatccgagtg
     1981 tagcgtatgc gtttgttgat gaaggctgtt atggagaaaa gggcttagac agcgtgagat
     2041 cgggtatgaa aaaagaggct attctttttt atctagactc tgttggggca gatacaccgc
     2101 tacagttttc gggaaattat ttttcaaata aagaacagtg gctgaaacaa gttgataagt
     2161 tgaaagaaaa aaacgtcaat tatattttta gtgcaagaaa aaagcaagcg cagttcttct
     2221 atttgaccaa aacagattta cgagggaaaa catttaattg gcaaaatgcc aatcagatta
     2281 ttgctctttt tcgataagta aaaagtgcaa aacaaggagt gagaagacta atgttggaaa
     2341 tcatgatcgt tacacatggt aagttgagcg aaggactgaa agatgcggcg gaagtaattg
     2401 tcgggacatc gagccatatt gaaacaatgt gcttagagtc tggggaggac atccaaacat
     2461 tcggagagaa ggtcaaagaa aaattaacta tgatcaacac agcagaaccc atgctagtat
     2521 ttgttgatct aattagcgca agtccgtaca atcaagcgct cttggcagtc aatgaactga
     2581 ctgaagaaaa gaaagcagcc acttatgtca tagggggcgt gaatctgcca atgttgttag
     2641 aagcaatcaa tcatcagatg cttcacacac cgatagaaga agcggtggaa caaattgtca
     2701 aacaaggaaa agataattta actgtttggc atgcttcagc ggaagaaaaa aaattagatg
     2761 aggacgattt ttagaaggaa ggctgaaaag atgaagtggg gatttagatg gtatggtgaa
     2821 aaaggggatt cgatcccatt aaaatatgtt cgccaaatac cgggaataaa tgggataatt
     2881 ggaactttgc tgactaaaat gcctggagat gtttgggaga tttcagaaat tgaggagttg
     2941 aaagcatctg ttgagagcca aggactggaa ctgcttggaa ttgaaagtgt agcgatccac
     3001 gatgcaatca aggcagggac agatgaacga gattactata ttgataacta tatacgaaca
     3061 atcaaaaact tgtctcgatg tggggttcga ttgatttgtt atagcttcaa accaattttt
     3121 ggatgggcga aaactgattt gtttaatgaa aacaacgatg gaagctattc tcttttatac
     3181 gatcagaaaa tcgtcgatca gatggagcca ggagaaatgt atcaattgat acacagccag
     3241 tcaaagggat tcaaattgcc gggttgggaa gaagaacggt tgaaaaaatt cgctcagctg
     3301 gaaaaactgt atgaaggaat gaccgaagaa gaattatttg aaaatctgac ttatttttta
     3361 gaaaaagtga ttccagtgtg cgaaaaagca gatgtaaaaa tggctatcca tccagatgat
     3421 ccgccatggg aactttttgg ttttcctaga gtgacgaaaa atcttggcga cttgaagaaa
     3481 attctaaatg cagtagattc tccatataat ggactaacgc tgtgcacagg atctttaggg
     3541 gcaaatccgg aaaacgattt agttgaaatc atccatgagg taggttcgcg catccatttt
     3601 gtccacttca gaaacgtgga gtttttagga gaaagaaagt tcaaagaaac ggcacatccc
     3661 tctacagaag gctcccttga tatgtatcat attatgaaag cgttggtgca agaagggttc
     3721 gacggtccta tacgccctga tcatggtaga accgtgtggg gtgaagtggc tatgcctgga
     3781 tatggcctgt atgatcgtgc aatgggactc acttacatgc aaggtctata tgaggcgata
     3841 agtaaggaaa aaccactggg agagcattat gaataaaatc aagctcttca tctttgatat
     3901 ggatggcctt ttattagaaa ctggacgttt agcttatcgt gcttatgtaa aatcagcaca
     3961 aaagtatgat tatgaaatgc gtaaggaagt ctattattta ctaactggcc aaacagaaat
     4021 ggcgataaga gaacaaatgg gcattttgta cggagaagat gttccatata tcaaatggcg
     4081 agaagcaatc aatcaatata aagaaaaaat cgtgaaagaa gataagagag tatatacgaa
     4141 aaaaggagca gaagaaattt tatcttttgc aaaagaaaga ggaatccata caatcgtggc
     4201 ttcgtctaat acacgggtaa aagtagaaat gtatttaaaa atggaaaatc tatatgcctt
     4261 gtttgatcaa atcatttcag gggatgatgt aaaaaaagga aagccggaac cagagatctt
     4321 tttgaaagct tgttcaaaaa tgaatattcc tccaagtgaa gcacttgtat ttgaagactc
     4381 gattgcaggg atcgaagcag caaggcgagc gggcattttg tcttttctca tcaaagatca
     4441 tttgataggg ttgcccgatc ataatgggaa gcataagttg aaagtagatg taagtttata
     4501 tgaatccaat aaagcagact atgaatttta tgacctgcat caagcaaaac gatttttaga
     4561 aagtaaaggg ttgtatctct aataaagagg actattttct agtcctcttt atttattgtc
     4621 ctgcacgact gagggaacag atgaaaaaga agtaattttt tattttgaag tatctacctt
     4681 atttgtagtg aacaatttag aacaaacaca aaacgcatct actgttatct accagtagat
     4741 gcgtttaaag agaagaacga gaatcgtcca tatgagggtt tccactcctt atgcagattt
     4801 ttttaattct tgcactgctt gttcgatcgt aacacctgtc gctacatgat tggtattttc
     4861 tttgtcaaaa acgacaaata gattcaaatc tgtatccaag gttactcgat attgtatatc
     4921 tttgttatag aatgtcatag ccattctcct ttctttaaat acttgctgaa aggcaacgaa
     4981 cgcgttgata acgaaattcc atttataagt ataccaccaa ttgaaaggtt ttcatagtta
     5041 agaaatttta aagtcaaaag ttgcagataa aagcaaataa acaagaaaac aaaaagtgaa
     5101 tcaatcgcgg ttattcaact ttttcttcga ttcgaagaca atagaagaca atgaatttta
     5161 tatttcattg cattatcctt gtgttattag aaaaagcatg atatattaaa tgagtaatct
     5221 attttgaaac gtaatttgag cgatatatct acatagataa aactcctttt gcctatcaaa
     5281 tatttattgc aacgaaaaaa cgtacattga tacgtatcag gaggaattta tttatgaata
     5341 acggtacagt aaaatggttc aacgcagaca aaggttttgg ctttatcact ggtgaagatg
     5401 gaaatgatgt atttgcacat ttttcagcta tccaaggtga cggcttcaaa actttagaag
     5461 aaggccaagc agtgtcttac gatgttgaag atggtcaacg tggccctcaa gcaacaaaca
     5521 ttgttaaagc ttaataaaaa ttgactttta accgtctttt cgaaagaaaa gacggttttt
     5581 ttgtacgaaa aaatt
//
LOCUS       DAZKKW010000127         5585 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.129, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000127 DAZKKW010000000
VERSION     DAZKKW010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5585
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.129"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..175)
                     /locus_tag="ACG8PO_002604"
     CDS             complement(<1..175)
                     /locus_tag="ACG8PO_002604"
                     /inference="COORDINATES: protein motif:HMM:NF016901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0207866.1"
                     /translation="MRTIQRKLRELNTILKNYHLSCSLKKGNPIEGEEYRIRYFFHLM
                     YWQSFDENHSDILS"
     gene            complement(178..534)
                     /locus_tag="ACG8PO_002605"
     CDS             complement(178..534)
                     /locus_tag="ACG8PO_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0207867.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            complement(832..1806)
                     /gene="sgrA"
                     /locus_tag="ACG8PO_002606"
     CDS             complement(832..1806)
                     /gene="sgrA"
                     /locus_tag="ACG8PO_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HFQ0207868.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            complement(2002..2841)
                     /locus_tag="ACG8PO_002607"
     CDS             complement(2002..2841)
                     /locus_tag="ACG8PO_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HFQ0207869.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNRTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            3026..3913
                     /locus_tag="ACG8PO_002608"
     CDS             3026..3913
                     /locus_tag="ACG8PO_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFQ0207870.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEGAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            complement(4507..5202)
                     /locus_tag="ACG8PO_002609"
     CDS             complement(4507..5202)
                     /locus_tag="ACG8PO_002609"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFQ0207871.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
     gene            complement(5186..5584)
                     /locus_tag="ACG8PO_002610"
     CDS             complement(5186..5584)
                     /locus_tag="ACG8PO_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFQ0207872.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
BASE COUNT         1784 a         1113 c          820 g         1868 t
ORIGIN      
        1 ttgataatat atcagaatga ttttcgtcga atgactgcca atacatcaag tgaaaaaagt
       61 atcggatacg gtattcttct ccttctatcg gattaccttt tttcaagctg caagataaat
      121 gatagttttt caaaatagta ttaagttctc ttaattttct ttgaatcgta cgcaaactta
      181 tataattttt tgactaagat tttctaagga ttgataagta cctttgtaaa gtgaatccag
      241 taaaatataa ccaatcgttc cctctaaaag tatagaataa actttactca gtaaaaactg
      301 agaagaaaac tcgactttta tcatcttgtt ttctgaaaga aaagataatt tatattctgg
      361 atacctttcg caaaacgatt ctgcttgttg aatcaccgaa ttcatcttag ccagggtgta
      421 gtttagatct aaagcgatat ccttcaccac atataatttc tcgtctcttt cataacaata
      481 gttcaaaaat tgatatactt gcctttcatt gatatcaata aattcttcaa acaaagtaca
      541 tactcctttt tatctattat ttagacttat tccgacagct acatgaataa cgtacaaata
      601 gattcgttac tgatttttca gagatactac catgcgcagt gtgcattgtc aattattctt
      661 gctcttattt tccatttgtg ttcctgttat ttcctgtttc tcaaatatgt tagtaaacaa
      721 aaatagatct ggcaaagcta caacttcgcc aggcaataca gcactctcga atgaaatatt
      781 cactcaaaaa tctatcgttt tattcaattt tagatctgtt gcttttattc tttatttcac
      841 tcgatggaag agagaacgaa aatagcttgc tcctacaatc aaaatgagta acacacccat
      901 ccatgtaatt agcttgttca aggttctact accagtttct ggtaaatcgc tttttctatt
      961 atctgtttct ttcgcttttt cttgatgatc aattccacga gaaacaaaat caggctgttt
     1021 tagcacactt tttaggttgc tccctgcatt tctcttttgt tccttagttg gtatgatagg
     1081 taccaaatca agttcattat ttacttttcc tgtgctgctt tcgctgctcg tgcttcctgt
     1141 gctactctca ctactcatgc ttcctgtgct actttcacta ctcgtactct ccgtactgct
     1201 ttcgctgctc gtgcttcctg tgctactttc actactcgta ctctccgtac tgctttcgct
     1261 gctcgtgttt tctgtgctac tttcactgct cgtactctct gtactgcttt caccgctcgt
     1321 gttttctgta ctgctttcac tacttgtgct atccgtactg cttggcggtc caacaactgt
     1381 ccaactgctt ggtttcaaat tctccgtgat cgtcatctgt ccatcataaa aaacaatcaa
     1441 atcaacttta tctttactct tgatctcatc aatcgatagt tctgcagaaa tcattattgt
     1501 atactcaata ccagcggtcc attgtacgtc ttttaaataa acttccccaa taactttttc
     1561 tgaacttcca ttaccatccc aaccatatgt tagaacagca tgtacatcac tagcattcac
     1621 gcttttgttt ggtttgactg gaatagtaat gacttgttgt gtcatgtcat aaacaatccg
     1681 atcagttttc acagtaaatg aaagattctc atttgcccgt tcatttgcag aagtactgat
     1741 tccaatcatt gctgcaatta tactcagccc aagaataaca aataatacgg tcgctgtttt
     1801 cttcatttct atcactctcc atataattac cttctatttg ataaaggaat tatataaaat
     1861 gaaaatgatg agaacacgac agtcagttgt gaaaatctaa caaaagatgt cgtgtattcg
     1921 cttgaatttg tttacttttt cgtttaatca atgacagact taaaaaagca agaccgatat
     1981 cagccttgct ttcttcatct tttattgata aacagcagtg tatttcttca tccgctttgt
     2041 tttatatgca tagagtaaca aaccaacaat caagactaac aagaataatg tagttgatat
     2101 agatgcttca gccccaaagt ttcccccagt aattattgta ttttgcgatt ggatcagagt
     2161 ggagaaaaaa ccttgttctt gcgttccgct gactggtata cccaaaatag ctccttgtgt
     2221 ccgattccaa gtaatatgag caccaatagc cgtaaataaa cttcctgtac tgtagataag
     2281 tgcgctgaaa aagcaacctg ctaaaaagat attgaaaact gccagaaatg tcatgttagg
     2341 attcaatccg tgcaaaagag caaataatgc agcagatcca atcattgcta ttaacggacc
     2401 aaatctaagc atctgatgct gaaaaaagcc acggaataaa agctcttctg atgcccccac
     2461 tgcaataaat aaaataaaac tcgttatcag cggcacaatg tttgcgtttc ctgaatctaa
     2521 ccgatacgcc cccgtcaaat ataatggact agtaataagc agaaacactc caattccaac
     2581 aacgagacct cccgctaaat acccccagtc ttttcctcga acagaaacac cataatcttc
     2641 tattcttttt ccttcgacct tactagcaag aaataaagct agtaaaaaaa cagctaaatg
     2701 tccgaccgta ccggcaattg ctccaatcca ttcactattt ccaataaaca tatccgcaag
     2761 tgcattattg acaagaattc ctaaaacaaa accaactaag actattaaaa cagatccaat
     2821 caaccgaata atcctgatca tctttcatcc ctcctttata ttaagtttaa caaccctttt
     2881 ctttcttgtc tcgtgaaaac tatgtaaatg ttctccatca tagagagaat agatttttac
     2941 gaactaagaa tatctacagc aaccttacga aaatgaaaga ctgtctccct aattcttctg
     3001 ttagactgtt tttaataagg aaaggatggg aaattttttg aaaaaacaac tactaattat
     3061 gtgcctttca gcatgtgcaa ttttactatt ctcaggttgt agcaagtcca atgaaacgat
     3121 tgcaacgttt aaaggtggaa cacttacagt tcaggatttt tatgataacc caaaagttaa
     3181 atcaaataat caaacactat taaaaaaaat gattgtcttc aaagcatttg atgatgtata
     3241 cggaaaagag atttcttcaa aggaagtaac aaaagaatat aatgaacaaa tcaaaaaact
     3301 cggtccaaac tacaaggaac aattaaaagc agttggccaa acagaagaga catataagaa
     3361 actctttaaa cagatgctgg ctttccaata cggtttgaaa gccaatgtaa aattaacaga
     3421 taaagatcta gacactgctt ggaaagaatt ctatccagaa gttagtacac agatcattct
     3481 attttcgact aaagaaggag cagataaagc aaaaaaagaa gcaaatgaag gagaaaattt
     3541 ctccaagctt gttcaagctt atggaaaaaa taaaacatta aaggaaactg atggaaaaat
     3601 gaattttgat tcaaccaatc cagagattcc tactgaagta aaaaaagcag catttaaact
     3661 aaaaaacggg gaagtttctg atatcattcc tgtaacagat ccaacgactt atcagcaaag
     3721 ctattatcta gtaaaaatgg taaaaaaaca ggataaaggt tctaataaag acaaatacaa
     3781 atctgagcta gaaaaaattg ctaccgaagc aaaaactctt gatacggaat tcatggataa
     3841 aacaatccgt aaagtgatga aaaaagacaa tgtaactatt aaagatcctt atgtaaaaaa
     3901 tatattcaaa taaacaaatt tgacccaatt ggggatgtgg caaaatttga gaagtgattt
     3961 ttggaaattc cttcttattt ttgaggcaaa ccacttttgc cacaacttca tcggggcctt
     4021 tttttatttg taccaaaaca aatcactgga tttgttacaa tcatgaggat ataatttaaa
     4081 aaagtttttt atttctatca catgaatcaa tgcgccatta agtagattct ttcatgcttc
     4141 cctatttttt cacttgccgt atcgcttcaa aaacaactat cataaactca aactcgactg
     4201 aaaagttatt ttaaaaaaga ctaaaaatat cccttctgga cttgttcttc ttattctgtt
     4261 atactagaga aacacgaaag atatttttat gcgacacttt tcatttgttt ttatgaaatt
     4321 tattcaaaac tggcgtaact tcagagttac aaggatgtat tagaggttag ggaatacatc
     4381 gtttaaatga gaaataagta atagttagta atagtcaccc aaactttaaa ttaatgaata
     4441 ttgaatcgtc agatagcctc ctcgagtttt cgagaaggct atcgtttatt ttatggagaa
     4501 attgattcag aagtaatcag cgaccttttt tagttcacca atcgtttctg cttggaaagt
     4561 ttgcacccaa tcttcttgga agcccctcac tgctttatca atcgagggca tctctaatac
     4621 ttgatctagt agctctttac ctaacgttat cgcatgagct ccagatcgaa tggctgtagt
     4681 gacttgttca caatttttga aactagctgc taatattttt gttggtcttt gcgtacgttc
     4741 gatctcagtc cgcaacgaat caatcacttt catagcatca atgccttgat tttgcatcct
     4801 gttataataa ggtgcaatat aatctgctcc attattaata gctaaatacc cttgtaattc
     4861 tgtgtaaata gctgtaccgg taatcaaata accttctttt ttcaactttt tgatcgcttg
     4921 aaggcctgct tgattcaccg gcaccttgat aaacgtatct tttcccagtt cttctatgat
     4981 aactcgagca tcctctatca tttcttcttc cgttcttcct attgtctgaa tatggatagg
     5041 aatctgtccg attatttttt tgatttcttt catatgcgag aagaagtttg acgctccttc
     5101 ttttttcaga attgtaggat ttgttgttac gcctgttaaa ttcaaaaact gttgacaaga
     5161 ataaatactt tttgtatttg ctgtatcaag catgatttcc atatagcttt cccccattct
     5221 gatcctcttg atcgatttta atgattgtcc cctttcttaa atgcggcatt tccttatctt
     5281 ctacatacat tgttcctgga agttccggct catgagatcc atcaaatttt atcgtgatat
     5341 gtcctaattc attcaaatta gtttggacaa catctcctac tgctgtgatt acatacattt
     5401 gtttatcaaa acagagcttc atatcacaag ttatttcttt tcttacctga ttcaattcaa
     5461 tcttataaca ataatctgtt aatgtttccg gtgcttcatc tccaaataaa atcattattt
     5521 tttctttcct gaatacttct gcttctatac caacatctgt tacaaccgtt tcaaagatcg
     5581 tcatc
//
LOCUS       DAZKKW010000128         5439 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.130, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000128 DAZKKW010000000
VERSION     DAZKKW010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5439
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.130"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            131..1300
                     /locus_tag="ACG8PO_002611"
     CDS             131..1300
                     /locus_tag="ACG8PO_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0207873.1"
                     /translation="MTKKIEVYVDGSQEERDSFFDFFWKNSREYVKALNLVYKQLMFE
                     ELIKDAIKEMDQGYAEREESIQNRIEKLAYTDPIDQKKLDKAKAELSKLRNEKNKEAR
                     EILTQAIGLKSQTRTRDVAKKLDFDFGDFVDSANMKASKDFGNDFIGIVTGQRTPRIY
                     KNRSKYGLLPFRGRDIKIHKDDKFYIYLPKGFRFKVNLGSKPRKARDVASVLQHIMDG
                     DYKLNQSNLIFDGRKLFFNLTISFEKRYNELEFKEGNELIVNFADLCSGECLVNNKSV
                     MSFGDSQYIRNFKSKILAMKSKEQSRGVFSKGGHGRKRKLDQDRWEAIKKRESNFVKT
                     YNNQCSAKIVKTAIRNKAQIIRIIEPDKAYGEWAYYQLKEQLVNKGKRYGIEVRS"
     gene            1817..3142
                     /locus_tag="ACG8PO_002612"
     CDS             1817..3142
                     /locus_tag="ACG8PO_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFQ0207874.1"
                     /translation="MFDYSREPVNDYFLIDMKSFYASVECIERNLDPMTTPLIVMSRS
                     DNTGSGLTLASSPTAKKKYHITNVSRATDLPYPLPKELHIVPPRMNLYIKKNMEVNQI
                     FRKYVADEDLLIYSIDESILKVTKSLNLFTSSGTRSERRRELAKIIKDEIQKETGLIA
                     TVGIGDNPLLAKLALDNEAKSSKEFIAEWSYETVPEKIWKINEMTDFWGIGSRMKKRL
                     NHMGIMSIEDLANWNPYTIKSQLGVIGLQLYFHTNGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPKDYTRREEVELVVKEMAEQVAIRIRQHNCKTGCVRLAIGTSILEEKSGFSHQMKI
                     PITDNTKELQNYCLFLFNKFYEGQEVRHIGVTYSRLVYTDSLQLDLFSDPRKKIAEEN
                     LDRIIDKIRQKYGFTAIIHASSMLEGARSITRSTLIGGHAGGLGGLHND"
     gene            3135..3482
                     /locus_tag="ACG8PO_002613"
     CDS             3135..3482
                     /locus_tag="ACG8PO_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0207875.1"
                     /translation="MIEYNDRGRLKWSGFYLSDHTERINADNVQRANHNLAKEQMTTE
                     EIAKILRTAVIKNRPVCIQKEERNAEGYYPDDVVGKIIGYDELGVFVEEEKVHYDEIR
                     NVSFAELLKWSQI"
     gene            3479..3661
                     /locus_tag="ACG8PO_002614"
     CDS             3479..3661
                     /locus_tag="ACG8PO_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0207876.1"
                     /translation="MNIIKKYEFGYITYEQLIEELWGYGQQLINEVGIDCFCFYIESG
                     AGYHKNRYYIFPYPPK"
     gene            3797..4654
                     /locus_tag="ACG8PO_002615"
     CDS             3797..4654
                     /locus_tag="ACG8PO_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HFQ0207877.1"
                     /translation="MEEKLIKKDEIIMGLLVPRANTYTIPLTKRQLIAKRYQRITRAI
                     NREFWNSESDTAHSLYVGSYGRGTAISTSDIDIIVELPMAEFDRFKNYLSNGPSKLLQ
                     VIKNAFQEILPNSDIRADGQVVKINFHDGIKFEIVPAFNEKDYWGESKGFIYPDSNMG
                     GNWKATNPKKEQEAMKLKNTKSNNLLYATCKHFRHVRDTEFTSYHLSGIVIDSFVYEA
                     MGNWKFVENNSGGQNISSVSYETALLEYYNSHKVMGGLNLYSPGSNQFVNSDSSIICL
                     EKVLKKIAL"
     gene            4674..5234
                     /locus_tag="ACG8PO_002616"
     CDS             4674..5234
                     /locus_tag="ACG8PO_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLATT domain-containing protein"
                     /protein_id="HFQ0207878.1"
                     /translation="MGDEKKRLEVQLREAYGRVTYTYTTHLKQADNIISDNKILRCLQ
                     IGLSALTTGGVLSTIITDNSELTFVAGLCSTISLGLNLYFKNFDLAEEAKNHQIVADK
                     LWLIREEYVSLLTDLNRLSVEDIVKKRDQLMNETHEAYKNAPKTNSKSYESAQKALKS
                     EEEQFFTEEELDKMLPEHLRKKSSKY"
BASE COUNT         2094 a          694 c         1016 g         1635 t
ORIGIN      
        1 gaaatttcta ataaagataa gatattttat ttagataatc atgtgaatat gagcttaaca
       61 attaaacgta tttatgatct tttatatgtt tataaacatt aatgaggtgt taaaattgac
      121 aacaataact atgacaaaaa aaatagaagt atatgttgat ggatcacaag aagaaaggga
      181 ttcctttttt gatttttttt ggaaaaatag tagagaatat gtaaaagcgt tgaatctggt
      241 atataaacag ctaatgtttg aagagttgat aaaagatgca attaaagaaa tggatcaagg
      301 ttatgctgag agagaagaaa gtattcaaaa tcggattgaa aagttagcat atactgatcc
      361 aatagatcaa aaaaaacttg ataaagcaaa agctgaatta tctaaattga gaaatgaaaa
      421 aaataaagaa gcgagagaaa tattaacgca agcgataggg ttgaagagtc aaacgagaac
      481 acgagatgta gcaaaaaaat tagactttga ctttggagat tttgttgatt ccgcaaatat
      541 gaaagctagt aaagattttg gcaatgattt tatcggaatt gtgaccggac aaagaacgcc
      601 tagaatatat aaaaatagaa gtaaatatgg attattacct tttagaggtc gagatattaa
      661 aatccataaa gatgataagt tttatattta tttacctaaa ggctttaggt ttaaagtgaa
      721 tttaggtagc aagccaagaa aagcaagaga tgttgcttca gttttacaac acattatgga
      781 tggcgattac aaattaaatc aaagtaatct tatttttgat ggaagaaaat tattttttaa
      841 tctaacaatt tcttttgaaa aaagatataa tgaactggag ttcaaggaag gtaatgaatt
      901 gatcgtaaac tttgcagatt tgtgtagcgg tgaatgttta gttaataata aatcggtaat
      961 gtcttttggt gatagccaat atataagaaa cttcaaaagt aaaattttag caatgaaatc
     1021 aaaagaacaa agccgaggag tcttttctaa aggcggtcat ggtcgaaaaa gaaaattaga
     1081 tcaagatcga tgggaagcaa taaagaaaag agaaagtaat tttgttaaaa cttataataa
     1141 tcaatgttct gcaaaaatcg ttaaaactgc tattcgcaat aaagctcaaa ttattcgtat
     1201 cattgaacca gataaggctt atggcgaatg ggcttactat caattgaaag aacaactagt
     1261 aaataaagga aaaagatatg ggatagaagt cagaagttag taaagtaaac aatcgttgat
     1321 ttggcaacga aatagtgagg gaaaatccac tcactgaagg gtaaactccg tcaagttgtg
     1381 tgaccagacg gaaggacgag cgttgttggg gttttagaac catataaaac agcatgtttc
     1441 tcaaattgtg tttgtttcat ttatgttata ataagagttt tagagccata taaaacagca
     1501 tgtttctcaa atgagtactt taaattaatt gatggcgttc ctgctttaga accatataaa
     1561 acagcatagt tctcaaattg tttaaaatga ttgtcaagtt aaaaaaatct gggatcatat
     1621 aaaatagtat agttctcaaa tatgattaga aaccctatta aagagcaata ttaagccaac
     1681 ttacttaaat cgtttaatta agaacagtaa tctcttttag tcagactttt agtctgactt
     1741 ttttgtattt ccaaaattta ttctttacaa aaacgaacat atgtttgtat aattgcttgt
     1801 gaatggggga ataaaaatgt ttgattacag tagagaaccg gtgaatgact attttttaat
     1861 cgacatgaag tctttctatg ctagcgtgga atgtattgaa cgaaatttag acccaatgac
     1921 tacaccgtta attgtcatga gtagatcgga caacactggt tcaggtctga ctctagcttc
     1981 ttcacctact gcaaagaaaa aatatcatat tactaatgtt agtagagcta ctgatttacc
     2041 ttatccatta ccaaaagaac ttcatattgt tccaccacga atgaacttat atataaaaaa
     2101 aaatatggaa gttaatcaga tttttagaaa gtatgtcgca gatgaagatt tattgattta
     2161 ttcgattgat gaatcaattt taaaagtgac aaaatcctta aatttattta ctagttcagg
     2221 aacaagaagc gagagaagaa gagaattagc taaaataatc aaagatgaga ttcagaaaga
     2281 aacaggtcta atagcaacag tcggaatagg agataaccca ttattagcta aactcgcact
     2341 ggacaatgaa gcgaagagta gtaaagaatt tatagctgag tggtcttatg agactgtacc
     2401 tgaaaaaata tggaaaataa acgagatgac tgacttttgg ggcattggat cacgcatgaa
     2461 aaaaagattg aatcatatgg gaattatgag tatagaagat ttagcaaatt ggaatcccta
     2521 cacaattaaa agtcagttag gtgtaatcgg acttcagtta tatttccata ccaatggaat
     2581 tgatcgtacg gatattgcta ttccaccaga accaaccaaa gaaaagtctt atgggaattc
     2641 acaagttcta ccaaaagatt atacgaggag agaagaagta gaactagtag tcaaggagat
     2701 ggcagagcaa gtagccattc gaattagaca acacaattgc aaaactggtt gtgtacgctt
     2761 agcaattggt acgtcaattt tggaagaaaa atcaggtttc tcgcaccaga tgaaaatacc
     2821 aattacagat aatacgaaag aattacaaaa ttattgtctt tttttattca ataagtttta
     2881 tgaggggcag gaagtacgac atataggagt aacttatagc agattagtat acacagattc
     2941 cctacaatta gatttattta gtgatccaag aaaaaagatt gctgaagaaa atttggatag
     3001 gattattgat aaaattagac aaaaatatgg atttacagct attatacatg cttcatctat
     3061 gttagaaggt gcaagaagta ttacgagaag cactttaata ggaggacacg caggaggatt
     3121 aggaggtttg cataatgatt gaatataacg atagaggacg tttaaaatgg tcgggatttt
     3181 atttgtctga tcatactgaa agaatcaatg cagacaatgt acaacgtgct aatcataatt
     3241 tggcaaaaga acaaatgacg actgaagaga ttgcgaaaat actgagaact gctgtcataa
     3301 aaaatagacc cgtctgcatt caaaaagagg aacgaaacgc tgaaggttac tatccagatg
     3361 atgtagtagg aaaaattatt ggatatgacg agcttggtgt atttgtggaa gaagaaaaag
     3421 ttcactatga tgaaattagg aatgtttctt tcgcggaatt attaaaatgg agtcagatat
     3481 gaacattatt aaaaaatacg aattcggtta tattacgtat gaacagctaa tagaggaatt
     3541 atggggatat ggtcaacaat taattaatga agtggggatt gattgctttt gtttctatat
     3601 tgaatccgga gcaggatacc ataagaacag atactatata tttccatatc caccaaaata
     3661 gtctcttaga gatttttaaa ttttctccaa atactttcaa ttattgtgaa atatttcctt
     3721 ttaattaatc aaatttttta taaaagtaat atataaaata aattgcttga agtattatat
     3781 ttagatatca taatttatgg aagaaaaatt aattaaaaag gatgagatta taatggggtt
     3841 actggttccg agagctaata cttatactat acctttaacg aaacgccaat taattgctaa
     3901 aagatatcaa aggattacta gagccatcaa tagagagttt tggaattctg aaagtgatac
     3961 tgctcacagt ttatacgtag gttcttatgg gagaggaacg gcaattagta ctagtgatat
     4021 agatataata gttgaattac cgatggcaga atttgacaga ttcaaaaatt atttatcaaa
     4081 tggcccgtct aagttattac aagttataaa aaacgcattt caggaaatac taccaaatag
     4141 tgatattaga gctgatggac aagttgttaa aataaatttc catgatggaa tcaaatttga
     4201 aatagttcca gcttttaatg aaaaagatta ttggggtgag tcaaaagggt ttatttatcc
     4261 agattcaaac atgggaggga actggaaagc tacaaatccc aaaaaagagc aagaagccat
     4321 gaaattaaag aatacaaaaa gtaataattt attatatgct acgtgtaaac attttcgaca
     4381 tgtacgagat acagaattta cgagttatca tctttcagga atagtaattg acagtttcgt
     4441 atatgaggct atgggaaatt ggaaattcgt tgaaaataac agtgggggtc aaaacattag
     4501 ttctgtatct tatgagacag ctttattaga atactataac tcacataaag ttatgggcgg
     4561 tttaaattta tactctcctg gaagcaatca atttgttaat tctgatagta gtatcatatg
     4621 tttagaaaaa gtattaaaaa aaatagcttt ataaagtttt atggaggaac aacatgggag
     4681 acgaaaaaaa gcgcttggaa gttcaattac gtgaagcata tggacgagta acgtatactt
     4741 acaccactca tttaaagcaa gccgataata ttatttctga caataaaatt ttaaggtgtc
     4801 ttcaaattgg tttgtcagcc cttactactg gaggggtact tagtaccatt attactgata
     4861 atagtgagtt aacctttgtt gctggattat gttcaacaat ttctcttgga ttaaacttat
     4921 actttaagaa ctttgatctt gctgaagaag ctaaaaatca tcaaattgtt gccgataaat
     4981 tatggttgat cagagaagaa tacgtaagct tactaacaga tttaaatcgt ttaagtgttg
     5041 aagatattgt gaaaaaaaga gatcaattaa tgaacgaaac tcatgaagca tataaaaatg
     5101 cccctaaaac aaatagtaaa agctatgaat cagctcagaa agcattaaaa tctgaagaag
     5161 agcaattttt tacagaagag gaattggata agatgttacc agagcatctt agaaaaaaga
     5221 gttctaaata ttgaactctt tttttatgct taaaattctc ttgtaatttt tctattcaat
     5281 caataaataa tctatattta agaagaaata tgatgataga aacatctcct gaagccaccg
     5341 caagccgttc taagcatagc ttagcccgac attgctagat atgctcatct caccatgaac
     5401 gtttcctaaa gccaccgcaa gccgtcctag ccattgcct
//
LOCUS       DAZKKW010000129         5433 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.131, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000129 DAZKKW010000000
VERSION     DAZKKW010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5433
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.131"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            190..690
                     /locus_tag="ACG8PO_002617"
     CDS             190..690
                     /locus_tag="ACG8PO_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFQ0207879.1"
                     /translation="MEQIRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELKGTPIGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCNDMSDVAYYLIVEAGDLGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFELM"
     gene            787..2634
                     /locus_tag="ACG8PO_002618"
     CDS             787..2634
                     /locus_tag="ACG8PO_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HFQ0207880.1"
                     /translation="MVGNKWYKCDLHIHSTASQCFQDKKVTAKEWVEECLNKGLDCVA
                     LTDHNTGENIDEYKKVAIENGLDFFPGVELTCGENGTHLLVLFDKSACTNDISDFLTK
                     MGIKREDWASAAACSMKSVLEVIDFAEHEGLLVIPAHIDEFNGISSAKFGNLGKIFES
                     ENIYAVQTVQKEFFENRSQMIPSSKRDTIYDSVNARYDGRVGKDTLESWYKSVVEAEK
                     NNMTFLSFSDNPHSKGNSKHGLWGIGTRYSYIKMKDEPDLGSLRDALMLGETRVHSDF
                     SNFSINENEVLLEKLSFSGTTLSTKEVVVEFSDNLTSIIGGRGTGKSCITRFLVYVLG
                     KEAELDQFSEIQSDYQNFAQIEHNGSGIFLKDTIVKLNIFYKGTKYQIIRTQDRHSIY
                     EFTQENGLVEKETERLRMISDKVSIYSQKQIYEISKNQTSILELLDGYNSDLISEYKN
                     EIETCVNEIRKLNWDIVSVKKEIQDKAKVELEIEDLKKQVEKLSHKSYKDVYDEYSKE
                     TDIYRELKRDVEALKEIPKNLTDSVDKIDFGNGIKVTDEIDEHRGELIKNLVTNRAKI
                     QTIINEMSDEIDSYRAELNKSDWKVNYNEVSKRYQRGIKNLVKSYLKMN"
     gene            2637..3533
                     /locus_tag="ACG8PO_002619"
     CDS             2637..3533
                     /locus_tag="ACG8PO_002619"
                     /inference="COORDINATES: protein motif:HMM:NF024700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0207881.1"
                     /translation="MLQNLDSLYQSLSKTEERLEEIIQQEIKLKDYQVEVDSAMSRMN
                     KSYQNLYSERSSSSREIFSDITGIEATIQPNRDFNNYVKELRACINKEDTFDSEFGSL
                     IDKLTKNSITNQDIYNDILDIRVSGESSIFSDKKLINSIRKLNDAQVLELQTLKPNDL
                     VVIRLELNGKKVSLSNASAGQKTSAILSMILAYGNSPLILDQPEDDLDSQLINNLIVR
                     SIITKKENRQILIVTHNANIPVNGDSEWLISMGDSKEISADQCGSVDEPNIKPRICTV
                     MEGGEDAFTNRAKRYGFKQIVE"
     gene            3583..3972
                     /locus_tag="ACG8PO_002620"
     CDS             3583..3972
                     /locus_tag="ACG8PO_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFQ0207882.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEEVFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPSTLVRSVNYVSD"
     gene            3959..>5433
                     /locus_tag="ACG8PO_002621"
     CDS             3959..>5433
                     /locus_tag="ACG8PO_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFQ0207883.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELVPYNYSFLSPEQKFQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLKEVAKQRIDIQTDALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKSLKKSFFSGLQEFVYGVNHHLMGDFVSLSNEEI
                     RCYSKLEKLMENKLARRFKVRRVTPSDLTYLIEHIYGEKGIPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPNRCLIEEKPRCLRMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKGSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGG"
BASE COUNT         1857 a          787 c         1151 g         1638 t
ORIGIN      
        1 caatctttag tcttactagc gattgtggtc attggtttgt ttttaggatt taaacgcgag
       61 ttttccaaac tgattggctt cttggtgatt gctttgattg cggtaggtct ggtctttaat
      121 gcttctggtg tgaaggatgt cttactcaat ttgtttaacc gaattgtcgg tgcctgaggg
      181 ggaatcacaa tggaacagat acgtgtttat attgcgaacc ttggaaaata caatgaaggt
      241 gagttggtgg gggcatggtt taccccaccc gtcgattttg atgaagtgaa agaacggatt
      301 ggattaaatg acgaatatga agagtacgca attcatgatt atgaactgcc ttttgagatt
      361 gatgagtata caccaattga ggaaattaat cggttgtgtg gtttagcgga agagcttaaa
      421 ggaacgccaa ttggtgaggt cgcttcagag attcaacatg ccttcttcaa ttctttcgag
      481 gaaatggtag agcatgtgga tgatatcatc tattatcccg attgtaatga catgagtgat
      541 gtagcgtatt acttgattgt agaagctggt gatttaggcg aagtaccaga acacttgaag
      601 acgtatattg attttgatgc gtgtggtcga gacttggaga ttggcgggaa ttatttggtt
      661 acgagtcgag gggtttttga actaatgtaa atcatttgtt agaattgacc agaaatttct
      721 atcgtacttt tacttttcta tttgatatac tgaaatagaa aatatttgtt gataggggtg
      781 ttactaatgg ttggcaataa gtggtacaaa tgtgatcttc atattcattc aactgcgagt
      841 cagtgctttc aggataaaaa ggttactgct aaggaatggg tagaagaatg tttaaacaaa
      901 ggactcgatt gtgttgcatt aactgatcac aatacagggg aaaatattga tgaatataag
      961 aaagtggcta tcgaaaatgg cttagatttt tttcctggag tggaattaac gtgtggagaa
     1021 aatggaactc atttactagt cctatttgat aaaagtgcat gtacaaatga tatctctgat
     1081 ttcttaacca agatgggtat aaaaagagag gattgggcca gcgctgcagc ttgctcaatg
     1141 aaatcagttt tagaagttat tgattttgct gagcacgagg gcttgctcgt tattcctgca
     1201 catatagatg agtttaatgg aatttcttca gcaaaatttg gaaatctagg aaagatattt
     1261 gaatcagaaa atatatatgc agtacagacg gttcaaaaag aattttttga aaatcgctca
     1321 caaatgattc cctcatctaa gcgtgacact atctatgata gtgtgaatgc cagatatgat
     1381 ggtagagttg gaaaagatac tttggaatct tggtataaat cagttgttga ggcagaaaaa
     1441 aataatatga catttcttag cttttctgat aacccacact cgaaaggaaa ttcaaaacat
     1501 ggactttggg gaataggcac aagatattcc tatataaaaa tgaaggacga acctgacttg
     1561 ggatcacttc gcgatgcttt aatgctaggc gaaactcgag ttcattctga tttttctaat
     1621 ttctccatta atgaaaatga agttttattg gaaaaattaa gcttttctgg gaccactctt
     1681 agcacaaaag aagttgtagt cgaattcagc gataatttaa cttcaattat tggaggaaga
     1741 ggaacaggca aatcttgtat tacaagattc ttggtctatg tccttggaaa ggaagcagaa
     1801 cttgatcaat tttctgaaat acaaagtgat tatcagaact ttgcacaaat tgaacataat
     1861 ggctctggaa tatttttgaa ggatacaatt gtgaaactga atattttcta taaggggaca
     1921 aaatatcaaa taataagaac acaggataga cattctattt atgaatttac acaagagaat
     1981 ggtctggttg aaaaagaaac agaacggttg agaatgattt ccgataaggt gagtatatac
     2041 tctcaaaaac aaatttatga aatttcaaaa aatcaaacta gtatactaga attactagat
     2101 ggatataact ctgatttgat ttcagagtat aaaaatgaga ttgaaacatg tgtaaacgaa
     2161 ataagaaaat taaattggga tattgtttct gtgaaaaaag aaatacaaga taaggcgaag
     2221 gtagagcttg aaattgaaga tttaaaaaaa caagtagaaa agctatctca taagagttat
     2281 aaagatgttt atgatgagta ttcaaaagag acagatattt atagggaact gaaacgtgat
     2341 gtggaggcac ttaaggaaat tcccaaaaat ctaactgata gtgttgacaa aattgacttt
     2401 ggcaatggta taaaagttac agacgaaatt gatgaacacc gcggagaatt gattaagaat
     2461 ttagtgacca atcgggctaa gattcaaaca ataattaatg aaatgtcaga tgaaattgat
     2521 agttatagag cagagctgaa taaatcagat tggaaagtta actataatga ggtttcaaag
     2581 aggtatcaaa gaggtatcaa aaacttagtg aagagttatc tcaagatgaa ttaaaaatgt
     2641 tacaaaacct tgattcttta tatcaatctt tatcaaaaac tgaagagcga ttggaagaaa
     2701 ttattcaaca agaaataaag cttaaagatt accaagttga ggttgactca gcaatgtcaa
     2761 gaatgaataa atcatatcaa aatctctatt ctgagagaag tagttcctcg cgcgaaattt
     2821 tttctgatat tacaggaata gaggctacta ttcaaccaaa tagggatttc aataattatg
     2881 ttaaagagct cagagcttgt attaataagg aggacacttt tgacagtgag tttggatctc
     2941 ttattgataa gctgacaaaa aattccatta ctaatcaaga tatatataat gacatacttg
     3001 atataagagt tagtggggaa agtagtattt tttcagataa aaaattaatc aattctataa
     3061 gaaaattgaa tgatgcacaa gttcttgaat tacaaaccct taagccaaat gatttagtag
     3121 ttatccgact tgaactaaat ggaaaaaaag ttagtttatc gaatgctagt gctggtcaaa
     3181 agacatctgc aattctttct atgattttgg cgtatggaaa ttcaccactc atattggatc
     3241 agcctgaaga tgatttggat agtcaattaa ttaataactt aatcgtaaga agtatcatta
     3301 caaaaaagga aaacagacaa attttaattg taactcataa tgcaaatatc ccagttaatg
     3361 gtgattcaga atggttgatt agcatgggag attcaaaaga aatatcggct gatcagtgcg
     3421 ggtcggtaga tgaacccaat atcaagccaa gaatatgtac tgtgatggaa ggtggcgagg
     3481 atgcttttac aaatagagcg aaacgctatg gttttaaaca aattgtagaa taacactaga
     3541 gggtgagaca tattattatg cccgcccttt ttaggaggaa aaatgaaaaa aataaaaagc
     3601 tataccagta tctgggcagt tgaaaaagtc atttacgcaa tcaatgattt tcagttgcct
     3661 tttcctgtga cttttaacca aatggcttgg ttcgttttat ctttactgtt tgtcatcgta
     3721 tttgcccatg tgcctccact atctatgata gaggaggtct ttttaaaata cttaggcata
     3781 ccggtggctg tcacttggtt tatgtcgcaa aagacgtttg atggcaagaa accactggga
     3841 tttttacgat cctttatcag ctatcacttg cgatttaagg tgacttttgc aggaaagaaa
     3901 gtcaaagaac agaagaaacg atgggatgaa cccagtactc ttgtaaggag tgtgaactat
     3961 gtatccgatt aagtatattg aaaataatct cgtctttaac caagaaggcg agtgttttgc
     4021 ctactatgaa ttagtgcctt acaattactc ctttttgtca cccgaacaga aatttcaagt
     4081 gcatgataac tttcgccaac taattgcgca aaatcgtgaa ggcaagatcc atgcgttaca
     4141 aattgccaca gaaagtagta ttcgcgccac acaagaacga tcaaaaaaag aaattaccgg
     4201 tagactcaaa gaagtagcga aacaacgaat tgatatacag acagatgcat tagtatccat
     4261 gattggcgat agtcagattg attatcgctt ttttattggc tttaaactaa ttgcgacaga
     4321 tgaagaagtc aatctaaaga gtctcaagaa atctttcttt tctgggcttc aagaatttgt
     4381 ctatggagtg aatcatcatt tgatgggcga ttttgtttcc ttatccaatg aagaaattcg
     4441 ctgctattct aagcttgaaa aattaatgga aaacaaattg gctcgaagat ttaaagtcag
     4501 gcgagtcaca ccgagtgacc tgacgtattt gattgaacat atttatggcg aaaaagggat
     4561 accgtttgaa gaatacgaat ttcagctacc aaagaaaaag ctaaaatcag aaacactggt
     4621 gaaacgttat gacttattac gtccaaatcg ttgtttaatt gaagaaaaac caaggtgctt
     4681 acggatggaa catgagaatc atgagtcgta cgtagcgtat ttaacaatta atacaattgt
     4741 gggggagatg gaatttcctt cctcagaact tttctattac cagcaacaac aatttacatt
     4801 cccaatcgac acaagtatga atgtagagat tgtgacgaat aaaaaggctt tagctaccgt
     4861 tcgtaataag aaaaaagaac tgaaagattt agacaatcat gcttaccaat ctgacaatga
     4921 aacgaattcc aatgtcttag atgctttgga ttccgtggat gaattggaaa ccaccttgga
     4981 tcagtcaaaa gaatctatgt acaaactgag ttatgtggtt cgagtgagtg ccgaatcagt
     5041 ggatgaacta aagcgacgct gtgatgaagt gctcgatttt tatgatgata ccaatgtaaa
     5101 actagtccgg ccctttggcg atatgatggg gctacatgaa gaattcttac cctcaagtaa
     5161 acgatatatg aacgactata ttcagtatgt tacgtctgat tttttggcgg ggcttggttt
     5221 tggggctact cagatgctag gagaattaga agggatttac tttggctaca atgtggatac
     5281 aggtcgtaat gtttacctga aaccggcatt ggcttcacaa ggagtcaagg gttcagtaac
     5341 caatgcgctg gccgccgctt tcctgggttc tcttggtgga ggtaaatcct ttagtaacaa
     5401 tctcttagtc tactatgctg ttttgttcgg tgg
//
LOCUS       DAZKKW010000130         5339 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.132, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000130 DAZKKW010000000
VERSION     DAZKKW010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5339
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.132"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(19..414)
                     /locus_tag="ACG8PO_002622"
     CDS             complement(19..414)
                     /locus_tag="ACG8PO_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0207884.1"
                     /translation="MKNKGQIWYIGYIVSLFLIVTILVTDFPKMVDIGLAILFSAVFG
                     VSHVQILHDKMIKNDADYKINVMDERNIIIKEKAGNITNMFNTILLGVATVIFICLDY
                     IIPAIIVGSIIAIQPIILIIISSVLEKKM"
     gene            complement(404..616)
                     /locus_tag="ACG8PO_002623"
     CDS             complement(404..616)
                     /locus_tag="ACG8PO_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0207885.1"
                     /translation="MKNRLEELRKQRGIKQEDLASALEVSRQTIGSLENGRYNPSIIL
                     AFKIARYFQMSIEEIFIYEEESEDEK"
     gene            1688..2290
                     /locus_tag="ACG8PO_002624"
     CDS             1688..2290
                     /locus_tag="ACG8PO_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbiJ-NTD4 domain-containing protein"
                     /protein_id="HFQ0207886.1"
                     /translation="MEDIILRDRLTDPLKNSLANVIYDILREADKVYSNYNGKVSWVI
                     YSFLIKEFFKEQINNSYELGYHFTVYYEDIIKRLEDLEWYAYYDLLEVFASAKILDKK
                     KREEVNKILESENSGYRLSESNEFIPITDDISIKSINAVSESPFNYAKNHIHKSLTYI
                     SEKENQDYNTVVREAISAVESCLIEFSGLPANKKILWELL"
     gene            2645..2881
                     /locus_tag="ACG8PO_002625"
     CDS             2645..2881
                     /locus_tag="ACG8PO_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HFQ0207887.1"
                     /translation="MNIKKRKIREVGNSIVVTLSKESLLQKGLKPGDTIFIDEDKMME
                     AIVKEESKIDLEIDMYVNQALSEYDAAFKELVEK"
     gene            2878..3291
                     /locus_tag="ACG8PO_002626"
     CDS             2878..3291
                     /locus_tag="ACG8PO_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HFQ0207888.1"
                     /translation="MIYLSEKDIVKLNVIQIKKYSPRETIGVIDKNALSMAVNQPKQH
                     IFGKDLYPDKFSKAAVLLINLIKKHPFHNANKRTAFLAAYVFLKLNGYSLKMKNQEVV
                     EFVVEIATYQGDFDDLKEATTDILKKNSTKTDSKK"
     gene            3834..4760
                     /locus_tag="ACG8PO_002627"
     CDS             3834..4760
                     /locus_tag="ACG8PO_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HFQ0207889.1"
                     /translation="MTGRNPKVFISYSWENKENKEWARELADKLLENGIDAYIDQYDL
                     SLGDRLPQFMEQQISDSDYVLIICTPEYKKKANNRTSGVGYEGHIISAELLSGKNERK
                     FIPIIKEGRASEVLPTFLAGKLGIDLSDSQHYDENLDDLITTIFGVKRKPALGDIPRH
                     IKGSPLLPIEHNENKDIEILGIITDKVTVPKMDGSRGSALYKIPFRLNRVPSQLWKEL
                     FVRNWNSPPRYTTMHRPGIASVVDDEILLNGTTIEEVREYHRETLKLCIEKSNTQEKK
                     ILLEEEQKKERENERVRSHKESVQKLADELEF"
     gene            complement(4757..>5339)
                     /locus_tag="ACG8PO_002628"
     CDS             complement(4757..>5339)
                     /locus_tag="ACG8PO_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0207890.1"
                     /translation="SIDQNEQRQIEEMRKFGAEKIFIEKQSGATITHRPIFQEVLDFV
                     RDQDIFIVEAIDRLGRNYDEIIDSVNYLKKKNVRLIITNLPIMAEAIGNPLLDKFIKD
                     LIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANTKDPQRRLVYRSIV
                     QDQENGVAISKIATDYNVTRQTVYRIKKELTIN"
BASE COUNT         1908 a          760 c          902 g         1769 t
ORIGIN      
        1 ttgtatgaac ctttatttct acattttttt ctcaagcaca ctactgataa tgattaaaat
       61 aatcggctgg attgcaatga tacttccgac aataatagct ggaatgatgt agtctaaaca
      121 aataaatata actgttgcca cccctaacag tattgtgtta aacatatttg ttatatttcc
      181 cgctttttct ttgattatga tattccgttc gtccataaca ttgattttgt agtctgcatc
      241 atttttaatc attttatcat gcaaaatttg aacatgagaa acgccaaaaa cggcactgaa
      301 taaaatagca agtccaatat ctaccatttt aggaaaatct gtaaccaaaa ttgtcacaat
      361 caaaaacaac gaaacaatat aaccgatata ccaaatctga cccttatttt tcatcttcac
      421 tttcctcctc atagataaaa atttcttcaa tgctcatttg aaagtacctt gcaattttaa
      481 atgccaatat aatagaggga ttataacgtc cattttctaa agaacctatt gtttgacgag
      541 atacttcaag agcagaagct aaatcttctt gtttaattcc tctttgtttt cgtaattctt
      601 ccaaacgatt tttcacaaca tgctcctttc taaaaaatgg aaagtttact ttcctttcgt
      661 gcttttataa tactcctgcg agcacaaaat gtcaaggaag ctttccatat aaattttgat
      721 tttaatgttt tagttactgt ttttatgaca catgaacaca cttattctta tttcctctta
      781 tctgctatca acagaaaaga aaaagccctg tcaagagccg attagatcaa gccagtcgag
      841 aaacgatgtt agaccaaatt gattttgggt aatgaatgtt tagaacttag tagtcatcca
      901 aaaacgacta ctttttttgt tgaatcgtcc aaggacgatt cgatgctttt gacttgtaag
      961 aacaggattg aatgaaacca tactgttcgc atatttttag tggatgaaca aacaaaatac
     1021 gagagatttt ttgttcgttc atccatggtt ttagaaaaaa gagggacgat tttggaagaa
     1081 gaaaatcgtc tctttttttt cttctttttg tatgacaaaa agaaagatct tttgcccatt
     1141 ttatttttat aaaatgggta ggtggcgttc gcgtaaagca aatcgataca atccaatggt
     1201 tttggcgaat cagatttacc attctaggct tgtttaatac agacatcgat tgttattttg
     1261 agaatttata tccttattgg caatgaaatg gatattctcc ataccaatag aaatcttcaa
     1321 gataattttc ggaaatatat aagagttaat caattatgat catatagtag gtaaaaatta
     1381 ggacagcctt caattatatg ggaaaccaat ataatcaagt aattattcgg gtaacacaaa
     1441 tactagagta ctcatttctt ataactgttt tacacctatt agggatcagt attattaata
     1501 gatctagata aattaatacg taattatata ataatttatg tttgtgccat taaacaatct
     1561 taacagatat ttcattttat ttatgtaaaa caacacgaat aattattgct tcgtaaagtt
     1621 atatgagtat aagttaattt actggaggta gtgcaatgct tttttctgaa agatttggtt
     1681 ttaaaaaatg gaggatatca tactgagaga tagattgaca gaccctctta aaaatagttt
     1741 agcaaatgtg atttatgata tattacgaga ggctgataaa gtttatagta attataatgg
     1801 aaaagtctca tgggtaatat attctttttt aattaaagag ttttttaaag aacagataaa
     1861 taatagctat gagttgggtt atcattttac tgtttattat gaagatatta tcaagaggtt
     1921 agaagacttg gagtggtatg cttattatga tttattagag gtttttgcaa gtgctaaaat
     1981 cttggataaa aagaaaagag aggaagtaaa taaaattctg gagtcggaaa attctggtta
     2041 cagattaagc gaaagcaatg aatttattcc aatcacagat gatatttcta ttaagtctat
     2101 aaatgctgta tctgaatcac catttaatta cgcaaaaaat catatccata aatctcttac
     2161 atatataagc gagaaggaaa atcaagatta taatactgta gtgagagaag caattagtgc
     2221 tgtcgaaagt tgtcttatcg aattttcagg attaccagct aataaaaaaa tactttggga
     2281 actgctataa aagcaattaa agacaaatcg aataacaatg tcgatcttac atttttaaaa
     2341 ccatttgaga caatgtatgg tttggcgagt aataatggaa tacgacatgc agaaaatgaa
     2401 aaaacaattg tctcagattt acctgatgca gttttggtgt taacaacatg ttcagccgta
     2461 attaattatt tgggagtaaa aatagcatat gactccaata aatcatgaaa agttaaaatt
     2521 catccaagag ctacattata actattaatt tccaactctt taaaagttta gtaattgaag
     2581 gaagtaataa gggtgattga acaatacgga tatacggtgt atacttatcg taggagttga
     2641 tgatatgaat attaaaaaac gcaaaatcag agaagtaggt aattcaatag ttgtaacttt
     2701 gtcaaaagag agtttattgc aaaaaggttt gaaacctgga gatactattt ttattgatga
     2761 agataaaatg atggaagcta ttgtaaaaga ggaaagcaag atagatcttg aaattgatat
     2821 gtatgttaac caagctttat ctgagtatga tgcagctttc aaggaattag ttgaaaaatg
     2881 atctatctaa gtgaaaaaga tattgtaaaa ttgaatgtta ttcaaataaa gaaatattca
     2941 ccgcgagaaa caatcggagt cattgacaaa aatgcgctga gtatggctgt aaatcaacct
     3001 aaacagcata tttttggaaa agatctgtat cctgataaat ttagcaaagc agctgttctg
     3061 ctaataaatc tgatcaaaaa gcatccattt cataatgcta acaaacgtac agcattttta
     3121 gccgcgtatg tttttctcaa gctaaatggc tattcactaa aaatgaaaaa tcaggaagta
     3181 gtagaatttg tagttgaaat tgctacgtat cagggtgatt ttgatgactt aaaagaggca
     3241 acaacagata tactaaaaaa gaattcaaca aagaccgatt caaaaaagta gaagcaaact
     3301 agggattcag ttaaatctga agatttagtt tgttctttat tttagtttac ataagataca
     3361 ttatgcgaag ttcattattt taaaagctta aataataaac ttttttataa taccaattta
     3421 tttatgtaaa ccaatgacct taatcgctta agcaagcatg ttatgtaata ctctactctc
     3481 tccaaaacta atgactatct attttcaaag aagctagaat gattatctga tcaaatgata
     3541 atttatattt aattcctggt ctactgctct tcttttaatc aattaatgtc taacgtactg
     3601 tacacgataa agtaggaaga cttattttca acttcggatt cattttttta attcggtgaa
     3661 aatcactaaa ttaagagttt atccaagttc attttagctg agaatacgct tgaaaataat
     3721 tttgatgaat tttcctgaat taaaatttat taacttgttg agtgatataa ttgtaatgta
     3781 ttaccaaata gttagcactt agattagtga tggaaaggaa tagtgagaac aagatgactg
     3841 gtagaaatcc caaagtattt atatcatatt catgggagaa taaggaaaat aaagaatggg
     3901 ctagagaact agcagacaaa ttacttgaaa acggtattga tgcatatata gatcagtatg
     3961 atttaagttt aggtgataga ttacctcagt ttatggagca gcaaatatcc gattcagatt
     4021 atgttctaat tatatgtaca ccagagtata aaaaaaaggc aaataatcgt acaagtggtg
     4081 ttgggtatga aggtcatatt atctcagcag aattgctttc tggaaaaaat gaacgtaaat
     4141 ttatcccaat aattaaagaa ggacgtgcaa gcgaagtact tcctactttt cttgctggaa
     4201 agcttggaat tgatttatca gatagccagc attatgatga aaacttagat gatttaatta
     4261 caactatatt tggtgttaag agaaaaccag cattaggtga tatacctagg catattaaag
     4321 gcagtccttt actacctata gaacataatg aaaataaaga tattgaaatc ttaggaatta
     4381 tcactgataa agtaactgta cctaaaatgg atggctcaag gggttctgca ttatataaaa
     4441 ttccatttag attaaataga gtaccaagtc agctttggaa agagttgttt gttagaaatt
     4501 ggaattcacc accacgatat acaacaatgc atagaccagg aattgcttct gttgtagacg
     4561 atgaaatttt acttaatgga acaactattg aggaagtccg ggaatatcat cgggaaactt
     4621 taaaactttg cattgaaaaa agtaatactc aagaaaaaaa aatattgcta gaggaggaac
     4681 aaaaaaaaga gagagagaat gagcgagtca gatcacataa agaaagtgtt cagaaattag
     4741 cagatgaatt agagttctag ttgatagtca gttctttctt aattcgatac acagtttgtc
     4801 tagttacatt atagtctgtt gctatttttg aaatagcaac accattctcc tggtcctgaa
     4861 cgatgcttct ataaacaagg cgacgttgag gatcttttgt gttcgcactg tataacttag
     4921 gacgtccttt atagacgccg tttgctttag caatctttat cccttgtgcc tggcgtcgtt
     4981 ttgattcagt acgctcttgt tcagcaatca tggccaagat ttgaatgatt aggtctttga
     5041 taaatttatc caggagagga tttccgattg cttcagccat gatcggtaaa ttagtgataa
     5101 taagtcgcac atttttcttc tttagataat ttactgaatc gataatctcg tcataattac
     5161 gtccaagtcg atcgatagcc tctacaataa aaatatcttg gtctctaaca aaatctagaa
     5221 cttcctgaaa aatcggtcga tgagtaatag tcgctccaga ttgtttctcg atgaaaattt
     5281 tttcagcacc aaattttctc atttcctcga tttgacgttg ttcattttga tcgatcgat
//
LOCUS       DAZKKW010000131         5218 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.133, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000131 DAZKKW010000000
VERSION     DAZKKW010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5218
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.133"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            554..2464
                     /gene="ltrA"
                     /locus_tag="ACG8PO_002629"
     CDS             554..2464
                     /gene="ltrA"
                     /locus_tag="ACG8PO_002629"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFQ0207891.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
     gene            2568..2792
                     /locus_tag="ACG8PO_002630"
     CDS             2568..2792
                     /locus_tag="ACG8PO_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0207892.1"
                     /translation="MSGTYLSIYQWFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFL
                     VIALIAVGLVFNASGVKDVLLSLFNRIVGA"
     gene            2805..3308
                     /locus_tag="ACG8PO_002631"
     CDS             2805..3308
                     /locus_tag="ACG8PO_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFQ0207893.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
     gene            3368..3757
                     /locus_tag="ACG8PO_002632"
     CDS             3368..3757
                     /locus_tag="ACG8PO_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFQ0207894.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            3744..>5218
                     /locus_tag="ACG8PO_002633"
     CDS             3744..>5218
                     /locus_tag="ACG8PO_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFQ0207895.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELIPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLTEVAKQRIDLQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKNLKKSFFSGFQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVRRVTPSDLMYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPSRCLIEEKPRYLCMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKDSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGG"
BASE COUNT         1797 a          836 c         1125 g         1460 t
ORIGIN      
        1 gctcggtaaa gacggctgaa agataccgta atagttgatg aacatatatc ttctatcgaa
       61 aactatccag aggtgggtag cgtatccaac aggtcggggg tctataaaat atctatggtt
      121 agaatgttct ataaggaact gacgaaacag cgaatgtacg ggtctaaatt tgaactttgt
      181 agaaatgcaa aggtacgaga aatcgtagct ggtgtggcaa agtaagaata taggttatga
      241 aatgccatat aacgttacag gcggtatcaa gccagcaggc ttatagctag tgacctaagg
      301 atatatatgt atagataaga ttatcggaac gaggaaaggt attgggttct catcaagaga
      361 ataaactgac gaagaacaat aagcagttga actaatgctg aaaagcagag ggcgaaccta
      421 agaaattctt gtaatgagaa tggaggaatg ccctcaagtc gtgtatatat tagaaaagta
      481 ttatgttgtt tcaataagga ttacgagtaa gataaaaaag ctcactcaat ttcaagtaaa
      541 ggagtgaagc cttatgccta aaaataaaaa tgaaacattg tgtgtagaag atttaagaca
      601 cgctgaatat tacgaaatgc aaaacacctt tgatgattta tatgccaaaa gtaagaatgg
      661 agatattttc actcatctta tggatattat cctatcgaga gaaaatatat tactggcata
      721 tcgaaatatc aaagcaaatg caggcagtaa aacagcagga acagacggta caataattaa
      781 agatattggc aaattgccag cagaaacagt tgtcaaaaaa gtaagataca ttgttgcagg
      841 cagtccacat gggtatcgac ctaagccagt gaggcgaaaa gaaataccaa aaccaaatgg
      901 taaaaccaga cctctaggta ttccttgtat gtgggacagg cttatacaac aatgtataaa
      961 acaagtatta gaaccaatct gcgaagctaa atttagtgaa aacagctatg gttttagacc
     1021 taataggtca gttgaaaatg caataaaagc aacctataac cgcctacaaa tcagccagct
     1081 acattatgtg attgaatttg atattaaggg tttctttgac aacgtgaacc attccaagct
     1141 gataaaacag atatgggcta tgggaatacg ggacaagcac ttgatattca ttttaaaaag
     1201 gatattaaaa gctccaatca aaatgactaa cggtacaata acatatccag agaaaggaac
     1261 tccgcaaggg ggaataatat ccccactact agcaaacatt gttcttaatg agttagacca
     1321 ttgggtagaa agccaatggc aggaaaatcc tgttacaaaa aactatgtgg ttcatatcaa
     1381 taaaagcggt agcccatgta aaagcaacgc ttacaaagaa atgaagaaaa caaagttaaa
     1441 ggaaatgtat atggttcgtt atgcagatga ttttagagtt ttctgccgct acaaggaaag
     1501 tgctgaaaaa gccaaaatag caatcactca atggattgaa caacgattaa aattggaagt
     1561 atcacaagaa aaaacaagaa ttgtaaatgt tagaaaaaga tactccgact ttcttggttt
     1621 caagattaaa atgataccga gaagaaagaa gttagttgtt aaatcacata taagtgataa
     1681 acaattcaaa aaccaaaaag acaagctggt atcacaggca aagaaaattg cttctccctc
     1741 aaaagggaaa acggaattag aagaaacaag actttataat tcaatggtat taggtatgca
     1801 aaattattat cagattgcaa cgaatgtaaa tctggattgt atgaaactga atagagctgt
     1861 aatggctatc ttcactaaca gattaggtac aaacaaaagg ggcagattga gaaaaacagg
     1921 cagacaactt acgacaaagg aggcaacaag atatggtaaa tctaaaatgc ttcggtatgt
     1981 tgctggcgag ggcgaaccta ttttccctgt tggatatatc caacacagaa aaccaatggc
     2041 aaaggtgtat ttagcgaatt gctacactct agagggaaga aaatttatcc acaccaatct
     2101 aagtgttgat aaattactga tgtaccagtt aatgaaatta tctttggaaa atcgcacaat
     2161 agaatacgca gacaatcgaa tttcgctatt ttctgctcaa catggaaaat gtgctataac
     2221 tctggaagaa tttcaacaag ctaatgaaat acattgtcat catattgtac caactggagc
     2281 tggtggaaat gacgattata aaaatcttat tttggtaaaa gaggcggtac accgtctaat
     2341 acacgctaaa acagaagaaa caatacaaaa atatatagct ctattgaagc taaataaagt
     2401 ccagctcata agactaaata aatatagagt attagctggc aatcaagaat taaacttgat
     2461 ataggtctac tacataaata cttttcaata taaacacgat ggaacgcgga gtgcggtgaa
     2521 agtcgcacgc tccgtgtgaa tggggggaaa agtttgagat atttataata tcaggaactt
     2581 acctatccat atatcaatgg tttttaacca atgcccaatc cttagtctta ctggcaattg
     2641 tggtcattgg tttgttttta ggatttaagc gggagttttc caaactgatt ggctttttgg
     2701 tgattgcttt gattgcggtc ggattggtct ttaatgcttc tggggtgaag gatgtcttac
     2761 tcagtttgtt taaccgaatt gtcggtgcgt aagggggaag cacgatggaa cagatgcgtg
     2821 tttatattgc gaacctcggg aaatataacg aaggtgaatt ggtgggggcg tggtttaccc
     2881 cacctgtcga ttttgatgaa gtgaaagaac gaattgggtt aaatgacgaa tacgaggaat
     2941 atgcaattca tgattatgaa cttccctttg agattgatga gtatacgcca attgaggaaa
     3001 ttaatcggtt gtgtggctta gcggaggaac ttgaaggaac gccagttggt gaagttgctt
     3061 ctgagattca acatgctttc ttcaattctt ttgaggagat ggtagagcat gtggatgata
     3121 ttatctatta tccagattgc gatgatatga gtgatgtagc atatttcttg attgtagaag
     3181 ctggtgattt tggagaagta ccagaacact tgaagacgta tattgatttt gatgcgtgtg
     3241 gtagagactt ggagattgga ggcaattatt tggtaacgag tcgaggtgtt tttgagtatc
     3301 catattaaca aaattattga agggtagttg atttctaaac tatccttctt tttaaaggaa
     3361 tgatttgatg aagaaaataa aaagctacac cagtatttgg gcagttgaaa aggtcattta
     3421 tgcaattaat gactttcaac tgccttttcc tgtgactttt aaccagatgg cttggtttgt
     3481 tttatccttg ctgtttgtca tcgtatttgc ccatgtgcct ccactatcta tgatagaggg
     3541 ggccttttta aaatacttag gtataccggt ggcagttact tggttcatgt cgcaaaaaac
     3601 gtttgacggt aagaaaccat tgggattttt acgatctttt attagctatc acttacgatt
     3661 taaggtcact ttcgcaggaa agaaagtcaa agaacagaag aaacgatggg acgaacccat
     3721 tactctggta aggagtgtga actatgtacc cgattaagta tattgaaaat aatcttgtct
     3781 ttaatcaaga gggagaatgt tttgcctact atgagttaat accttacaat tactcctttt
     3841 tgtcacctga acagaaatat caagtccacg ataactttcg tcaattgatt gcgcaaaatc
     3901 gtgaaggcaa gattcatgcc ttacaaattg ccacagaaag tagtattcgt gccacgcaag
     3961 aacgatcaaa aaaggaaatt accgggcgat taacagaagt agcgaaacaa cgaattgatt
     4021 tacagacaga ggcattagta tccatgattg gcgatagtca gattgattat cgctttttta
     4081 ttggcttcaa actgattgcg acagatgaag aggtcaatct aaagaatctc aagaaatctt
     4141 tcttctccgg ctttcaagaa tttgtctatg gagtgaatca tcatttgatg ggcgattttg
     4201 tttccttatc caatgaagaa attcgccgat ataccaagct tgaaaaacta atggaaagta
     4261 aattggctcg aagatttaaa gtcagacgag tcacaccgag tgatctgatg tatttgattg
     4321 aacatatcta tggcgaaaag ggtacaccat ttgaagaata tgaatttcag ctaccaaaga
     4381 aaaagctgaa gtcggaaact ttggtgaaac gttacgactt attacgtccc agtcgttgtt
     4441 tgattgaaga aaagccccgg tacttatgca tggaacatga gaatcatgaa tcatatgtag
     4501 cgtacttaac aattaataca attgtggggg aaatggagtt tccctcctca gaacttttct
     4561 attaccagca acaacaattt acattcccaa ttgacactag tatgaatgtg gagattgtga
     4621 cgaacaaaaa ggcacttgct accgttcgca ataagaaaaa ggaattaaaa gacttggaca
     4681 accatgccta tcaatctgac aatgaaacaa attccaatgt cttagatgca ttggattccg
     4741 tagatgaatt ggaaactacc ttggatcagt ccaaagaatc catgtacaaa ctgagctatg
     4801 tggttcgagt aagtgccgaa tcagtagatg aactaaagcg ccggtgtgat gaagtgctag
     4861 atttttatga tgataccaat gtaaaactag tccggccttt tggcgatatg atggggctac
     4921 atgaagaatt cttaccttct agtaagcgat acatgaatga ttatatccag tatgttacgt
     4981 ctgattttct cgccggactt ggttttggcg ccactcagat gttaggggag ttggaaggca
     5041 tttactttgg ttataacgta gatacggggc gcaatgttta cctgaaaccg gcattggcct
     5101 cacaaggggt caaggattca gttaccaatg cgctagcagc ggcattcctc ggttcactcg
     5161 gtggcggaaa gtcctttagt aacaatctct tagtttatta cgccgtcctt tttggtgg
//
LOCUS       DAZKKW010000132         5148 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.134, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000132 DAZKKW010000000
VERSION     DAZKKW010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5148
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.134"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..312)
                     /locus_tag="ACG8PO_002634"
     CDS             complement(<1..312)
                     /locus_tag="ACG8PO_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFQ0207896.1"
                     /translation="MTYTHLTPNELVMIEAYFHQETPVVIVAKQLKHGRQTIYNVYNF
                     LKCGGTALEYFEQYKENKRRCGRTEIIFPAEEKEYIAKRSTEGWTPDVIIGRAERTFS
                     CS"
     gene            472..1560
                     /locus_tag="ACG8PO_002635"
     CDS             472..1560
                     /locus_tag="ACG8PO_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0207897.1"
                     /translation="MYSKNWKKKLVMGVMVGSIFVFAGCSASTDKSKTATSETKTAEV
                     VKSKMKQIEKNVEKILAEKDKDTKLSEYKKFVTESSAYSKSDKTEKKITNTYTESIKK
                     IKTNFKEQDDKDLKENTLDNLEEATENDLSQKVNTLDELKKWVQKESTDVYSQAEVDS
                     LIKKIDDTITTYKNQLEKIKIDNQEATSTETTTESSEVATLQENVSEEPQVVHTNLLN
                     SIEEAAQYLGQKLPQSGGSDAFENGQQNSDGSFTFHYQLHGGPEGITWNIITVNNNGE
                     IIQDDYFKTVPNEATNNNVSTLSQAAISELEAAQLAIAKHNELGRPNATDAGSETTAD
                     GYYFYYMADGTDQMYKYFVSNSGQVQAE"
     gene            complement(2017..2184)
                     /locus_tag="ACG8PO_002636"
     CDS             complement(2017..2184)
                     /locus_tag="ACG8PO_002636"
                     /inference="COORDINATES: protein motif:HMM:NF012372.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFQ0207898.1"
                     /translation="MSIREIVRIQSFPDSYIFVGSSLSAKYKVIGNAVLPKLAKVIGD
                     SIVKQLSESNF"
     gene            2229..2651
                     /locus_tag="ACG8PO_002637"
                     /pseudo
     CDS             2229..2651
                     /locus_tag="ACG8PO_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(3428..3623)
                     /locus_tag="ACG8PO_002638"
                     /pseudo
     CDS             complement(3428..3623)
                     /locus_tag="ACG8PO_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
     gene            3972..5057
                     /gene="xerS"
                     /locus_tag="ACG8PO_002639"
     CDS             3972..5057
                     /gene="xerS"
                     /locus_tag="ACG8PO_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HFQ0207899.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLENNPMDQVATITDKTTLAARAN
                     AIEKKLFLDEDTQNYLDYIANEYKKTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VVNINLEDLSLEKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRVQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHTSTNTTTLYTHIDNAATIDALNAL"
BASE COUNT         1910 a          802 c          807 g         1629 t
ORIGIN      
        1 tgaacaagag aaggttcgct ctgcacggcc aataatgacg tctggggtcc aaccttcagt
       61 tgatcttttt gcaatgtatt ctttttcctc agcaggaaaa atgatttcgg ttctcccaca
      121 acgtcgctta ttttctttgt attgttcaaa gtattcaagt gctgttccac cacatttgag
      181 aaagttatag acattgtaaa ttgtttggcg tccatgttta agctgcttcg caacgataac
      241 aaccggagtt tcttgatgaa aatatgcttc tatcattaca agctcgtttg gtgtaagatg
      301 ggtataagtc atttatgttc actctccttg tatgctttag cgggtattac aatttgagtg
      361 taacataaat ggctttttta tttgtctcgc ttaattatac aaacggcgtc aatataacca
      421 cttttatgaa aagatgttat aattaactaa aaaataatag ggagaaaaca catgtactca
      481 aagaattgga aaaaaaagtt agtgatgggt gttatggtag ggtctatttt tgtttttgca
      541 ggttgttcag cttcgacaga caaaagtaaa acggctactt cagaaacaaa aactgctgaa
      601 gtagtaaaaa gtaaaatgaa acaaatagaa aaaaatgtag agaaaatttt agccgaaaaa
      661 gataaagata caaaactgtc ggagtacaaa aaatttgtta ctgaatccag tgcttactct
      721 aagtcagata aaacggagaa aaaaattact aatacctata cagaatctat taagaagatc
      781 aaaacaaatt ttaaagaaca agacgataaa gacttaaaag agaatacact agataatctt
      841 gaagaagcaa ctgaaaatga tctttctcaa aaagtaaata cattagatga actcaaaaaa
      901 tgggtacaaa aagagagtac tgatgtatat agccaagcgg aagtagattc attaatcaaa
      961 aaaattgacg atacaattac cacatataaa aaccaactag agaaaataaa aattgataat
     1021 caggaggcta catcaacaga gactacaaca gaaagtagtg aggtagctac tttacaagaa
     1081 aatgtttccg aagaaccaca agtggtccac acgaatttat taaattctat tgaagaagca
     1141 gctcaatatc tgggacaaaa acttccacaa tcgggcggtt cagatgcatt tgaaaatggg
     1201 caacaaaatt ctgatggctc gtttactttt cattatcaac tacatggagg tccagaaggt
     1261 atcacttgga atatcattac agtgaataat aatggtgaga ttatccaaga tgattatttc
     1321 aaaactgtac ccaatgaagc aacaaacaat aatgtctcta cacttagtca agcagctatt
     1381 tcagagttag aagctgctca acttgcaata gctaaacaca atgagttagg tagacctaat
     1441 gcaactgatg ccggaagcga aactacagca gacggatatt atttttatta tatggccgat
     1501 ggaactgatc agatgtataa atattttgta agtaattctg gtcaagttca agcagaataa
     1561 cattgagtta taatgatatt atcagatgaa ctgtatcata aatttttaac gtgaaatgtt
     1621 tgtaatatca attggagaat ggtatatgga tttactaaaa gataataact gttatttttt
     1681 agtaaattca tactatttag aaaagaataa tttgctgaat acttgtaaac tatgtgtgat
     1741 acttatctta tatggatata gactcgtggt gctagttaat ttcagcttat tataaaccaa
     1801 cattttcatt ttataaaaac gactaaaaac agaatttctg agtaaaattt caaaagtaaa
     1861 aatagtgata ttatgctctt gttcctagat tcaataatta actagcacca cgagtttata
     1921 aaattggcaa taaaatttta atcattatta acataacctg gatctgtaaa gcaatttaac
     1981 tcttaataaa aaagcatcta aaaaaagcgt gttcagctaa aagtttgatt cagaaagttg
     2041 tttaactata gaatcaccaa ttacttttgc aagttttggt agaacagcat taccaattac
     2101 tttatatttt gctgaaagag agctaccgac aaaaatataa ctatctggaa atgactgaat
     2161 tctcactatc tctcttatcg acactcttag atgaactcaa aaaaatgggt acaaaaagag
     2221 aatactgatg tatatagtca actagaaata tcgctcatag agtgcgtgtt cgaggtcgaa
     2281 aagaaatctg tgaggagttc aaattgattt accatgcgaa ttcaaaagaa gaggcattga
     2341 attacatcga ttttatgatt aggaaatgga aaaagcagga tcgaagagtc gtgaacctac
     2401 tcttgaatcc tgccctatta accttttata atttccctta cgctatcaga cgaacaattt
     2461 attcaacgaa cctgattgaa ggctttaata agcagctaaa acgatatact cgaagaaaag
     2521 aacaattccc taatgaagaa tctctctaga gattcttagc ttcgcaattc aactaatata
     2581 accagaaatt tttaagtaga gttcacaaaa gatttaaaga aattcaagat acattagagt
     2641 cgatgattta actgtaatta acagaatgga ttttccattt acacataatt cttgacgcta
     2701 cctcataata attaagagct ttaaaattag tatttattag atacttaaaa gctattttag
     2761 tattgataaa ttgttaccag caacaaaaaa gaagttactt agtgctaact tttgagcaac
     2821 tgctagagga ggtaggatat ggctaattat cctgtaattt ttattgtatt ttctagaaat
     2881 gtaattattc tcttaaacat tccagcatat ccaactatat agaaaattta cgttttctat
     2941 atagttggat gtttacaagt tatatctggt ttaataagtt tttttctgtt agtcatggca
     3001 tgttttattg tgctggttca tcaatatatt gaaatgtact aaaatgaaat tttcagttat
     3061 ggtataatat ttatgctgtc tgctaccccg gcctattatg gcaaggggag gaggttgtat
     3121 tatgaattac cttttcgata tgttttttgc aatcttagca gaagtatccg cgcattacat
     3181 ctgtaaatgg cttgattgca aaattattgc caaaaaagac agtaagccta cccgttaatc
     3241 tgaatagaac gtaaaagaaa accactagcc cgccagctag tggtttttgg tgtatcatga
     3301 attacctttt cgttaaaaca attataccat atttcctaaa aaatacaatt aatagacgaa
     3361 ttatcattac cactttgatg aaaaaagctg acataaattt ttcttttatt tttataatga
     3421 ataaacattt atcaataagc ttttgaaagg caggatcgta ctttgttgat gattgagata
     3481 ttgctacatc aatctcagca gttaagattt ttttgaattg ttgcttgaat tttttcttta
     3541 tctaaaaaaa ttgtatctcc aggatgcaac ccattttctt taagaaattc cttttaaatt
     3601 gttaatacta ctgagtgccc cacttctctg aattttcttt aacttctccc tccccttttt
     3661 tatttgtata tacattcata tacgcttgtt aatatcaaat caaccgtagt ctctcttatg
     3721 tctaattata tagaaagatt gttctttcta tataatttag acaatttaaa aaattaagat
     3781 tgcctccttc tgtttccttt cctattcatg tataccattt attttaaatt attaaatcta
     3841 tacctattta tctgcttttt tgaaataaat taataaattt cagagtatac caattgacaa
     3901 aacactctat aattggtata gtccaattat agagatactc ctctccccta cttatcgaaa
     3961 ggatgaataa catgaaaaat acagaaatat atcaaaaaat agatacaatc atacttcata
     4021 tgcccgatta tataaaagat tttgttcaag atcgtgagga taaagatcaa tcacctcgaa
     4081 ctactttaga atatctaaaa aattataagc tcttttatga atggcttctg agtgagtcta
     4141 tcgttcccga tacttcaatc acctcgataa gacagttaac tgcctatgat ctaaacttgt
     4201 acaaatctca tttgaaacgt cgcacaaagg aaaatgtaaa aaaagagaca acaaaaacta
     4261 agctcaaaga taataataac ttaggtctat ctacttctac tatcaataga aatatcacag
     4321 cattaaaggt tctatttaaa tatctttcga aaagttccaa taatccttta ggaaaaccat
     4381 acttagaaaa taatcctatg gatcaagtag caactatcac agataaaact acgttagctg
     4441 cccgtgcaaa cgcaatcgag aaaaaattat ttctagacga agatacacaa aattatctag
     4501 attatattgc caatgaatat aaaaaaactt tgagcaaacg agctttgatc tattatcaac
     4561 gagatgtaga aagagacctg gcgatcaatg cattgatttt aggatctgga ttaagactct
     4621 ccgaggttgt aaatattaat ttagaggacc tatctttaga aaagaataat gtggtagtaa
     4681 ctagaaaagg aaataagaga gacgcagtaa atatagcagc atttgcaatg gaatatttag
     4741 ctaattatct agctattcgg aaagaacgat acaaagtaac agaaaacgaa aaagctttat
     4801 ttctagcaat ttatcaagga gaagccaagc gtatatccgg tattgcaatc gaacggatgg
     4861 tagcaaaata ttcaaaagga ttcagagtcc aagtcagtcc acataaatta cgtcatactt
     4921 tggctactag actctatcaa cagacaaatt cattggtatt aactgcccaa caattgggac
     4981 atacaagtac aaatacaaca actttatata ctcatataga caatgcagct acaattgatg
     5041 ctttgaacgc tttgtaaatg tagtaaaaaa tagtataata atgaagcctg tctctaaaat
     5101 tagataaaag cccaacttta aaggcaagct tcattatcat ttttatat
//
LOCUS       DAZKKW010000133         5112 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.135, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000133 DAZKKW010000000
VERSION     DAZKKW010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5112
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.135"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(275..445)
                     /locus_tag="ACG8PO_002640"
     CDS             complement(275..445)
                     /locus_tag="ACG8PO_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0207900.1"
                     /translation="MTKKHLLFGSILLGILSFSPMLLSVANKGNKKGELKLNPNYYNS
                     QNEEVFVEKLDK"
     gene            complement(460..993)
                     /locus_tag="ACG8PO_002641"
     CDS             complement(460..993)
                     /locus_tag="ACG8PO_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFQ0207901.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(1014..1958)
                     /locus_tag="ACG8PO_002642"
     CDS             complement(1014..1958)
                     /locus_tag="ACG8PO_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFQ0207902.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVR
                     FTFDQLKDLSNYKATANVRFVEDLKATYEKILSLRFGRRSESGLSYDMFVMFTEFKIN
                     GDVENPYVDIRIYEKALPLLNNLDEWVRYSLQQFNELQSGYAKTMFRLLKQYRTKGFA
                     YFSKEDFLELLDIPKSYKQPDIDKRVIKPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKADDFNKHAPTKEDQNKALALADKQKEYEEPATTINEKIKQKKRELELL
                     QQIKQLEKEKKALEKQTESALSEKEKKSLLSDLDNLFQ"
     gene            2595..2681
                     /locus_tag="ACG8PO_002643"
     CDS             2595..2681
                     /locus_tag="ACG8PO_002643"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HFQ0207903.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(2802..3491)
                     /locus_tag="ACG8PO_002644"
     CDS             complement(2802..3491)
                     /locus_tag="ACG8PO_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0207904.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQT
                     EEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(3498..3776)
                     /locus_tag="ACG8PO_002645"
     CDS             complement(3498..3776)
                     /locus_tag="ACG8PO_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0207905.1"
                     /translation="MKEEQTTNKNYRSKTLQDIENEIKKQRAFLAPYTKERIQTTIDS
                     QVKKFHHALLNQRNKEQKKVIMQRSMDILAPLCCLIMTLLAFWLVWGE"
     gene            complement(3736..4650)
                     /locus_tag="ACG8PO_002646"
     CDS             complement(3736..4650)
                     /locus_tag="ACG8PO_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFQ0207906.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            complement(4632..5012)
                     /locus_tag="ACG8PO_002647"
     CDS             complement(4632..5012)
                     /locus_tag="ACG8PO_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HFQ0207907.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
BASE COUNT         1296 a          944 c          925 g         1947 t
ORIGIN      
        1 tatactacca agtaacgcct gtaccaaggg atccaggcgc tgattttgtg aatttttatt
       61 ggtctaggtt aaaagctctt tttaaggctt ggttttctgc ggttgtttct ttctaattct
      121 ttgtgaatat tgatcggtca ttttttaaat acttgttatt tttgtttgtg tttgttatgc
      181 tttttacata tctcatattc atttattatt cttttctaag gttaataata ggataatctc
      241 tttcagaaag actgctcgta cgagcagtct tttcttattt atcaagtttt tctacaaata
      301 cttcctcatt ttgactgttg tagtaattag gattgagttt tagttcccct tttttatttc
      361 ctttgttggc cactgataat aacattgggc taaagcttaa tattcctagt aggatagaac
      421 cgaatagcaa gtgttttttt gtcataagta tttccttctt catttccata gctgccacca
      481 ttttttccaa ttctttgttt gttcgtccat tgtttctgtt gcttcagaaa gagtcgttga
      541 actattctct gaagactctt ccgtataagt caactgcagt tgctcctgaa gtttttcgat
      601 ttgttggttg gcttgcaggg tgagttgttg ttgctggtcg agtagttttt gtatatgaga
      661 attattttgc tctttgaggg caagttgttc tcgtaaaaat tcattttctt cacgcaactg
      721 atctagcaac ttgtttgcaa ccacgttttt gtttggtata taagcatttt tatcgttgtt
      781 ttgttcatgt ttatgcaact tatctagcaa cctttgattg tctaagttgc gagcaagttg
      841 caatactcca gaacgcaata caaccttata accacgctca tttttagcta tcatggaaga
      901 atctaatttt tggatatgtt tcgatattgc ttgcctactc atcttaaaaa gatctgacaa
      961 ttcctttatt gtaaaggttt cttgtggttg catactggtt gcctcctagg ttgctactgg
     1021 aataaattat ctaaatcaga taataaactt tttttctctt tttccgaaag tgcggactct
     1081 gtttgttttt caagagcttt tttctctttt tctagttgtt tgatttgttg gaggagttca
     1141 agttctcgtt tcttctgttt gattttttcg ttaattgttg tagcgggttc ttcgtattct
     1201 ttttgtttat cagctaaagc caatgcttta ttttggtctt ctttggttgg tgcatgtttg
     1261 ttaaagtcgt ctgctttttt gatctcaggt ttgaacgtaa attgatagcc aataacaggt
     1321 tttccccgtc cttttccgta tttctttttg attgttagtc ctttgaagat tgcagttagt
     1381 tcttgacgta taggttttat aactcgttta tctatatcag gttgcttata acttttagga
     1441 atatctaata attcaagaaa gtcttctttg gagaaatatg cgaaaccttt ggttctgtat
     1501 tgctttaaca gacgaaacat agtctttgca tagccacttt gtagctcgtt gaattgttgt
     1561 aaagaatagc gtacccactc gtctaaatta tttaacaaag gcagtgcttt ttcgtaaatt
     1621 cgtatatcta cgtaaggatt ttctacgtcc ccgttgattt taaattctgt aaacattaca
     1681 aacatatcgt aagaaagccc agactcactt ctacgaccaa agcgtaaaga taagattttt
     1741 tcataagttg cttttaaatc ttctacaaat cgtacatttg cagttgcttt gtagttactt
     1801 aaatctttta gttggtcaaa cgtaaaacga actgtcttat cccctttttc acgcattcta
     1861 gacacgatgg aaaaaaacaa attcatttca accggtgtaa attttcttaa aggaattgta
     1921 tttaattcat ggtggtattt tacaatctca ttagccatat aatcacctca atccaattat
     1981 gaatgacatt ttttaaatgt caagacattc atatttgacg ttcaaacaga caaaatgtcc
     2041 ttcataaaca gacaaaatgt ccttcataaa cagacaaaat gtccttcata aacagacaaa
     2101 atgtccttca taacattctg tatccattgg tagagtaagg acacagctta cggtaaagag
     2161 gtttaaaaag aggtttaaaa agagatataa aaagaggggg gctaattttc ccgcctaaaa
     2221 accacttctt ttttctttaa acaggttcgc gcatttttca aaactgccaa tccaaaacct
     2281 tgcttcctac ggtcgccaaa aaggtgtgat cgctacgctc tcacattgta ctgcgcagtc
     2341 gcctaaaggc tcctttgcta aaaacatgtt ggtaaagagg cctttgagcg gttctgaatg
     2401 cttatctaag ccgttttaag acagtcttgg ataaaaatcc ttatctagct ttaaagcgcc
     2461 ttacaggcgg tcagggcaag ccctaacaac cctaaaataa gcgatactta aaaacaccac
     2521 acaagtttaa ttttttaaga tataataact tgtcgcctat ccgacagtct tgcgactgga
     2581 aggaggtgta tctaatgttt gaattgttca tttgccccct tcttatagaa gtttgtgtct
     2641 ctttattcag ttattggttg aataaccgaa acaagaagta aaatcaataa aggcgacatc
     2701 aacctgaaaa aaagccccag agaatccgac ctctctgggg ctttcgtgta tctaatgttt
     2761 gatcatttgc atgtttagtt taacatgatt gccgatcaaa ttcaattttg ctgttgctga
     2821 tctaaccatt ccttgacact ttttagataa tttaatgccg tttctttttt cgttggattt
     2881 tgtatgttcg ttgcttgctg aagcagtgct ttataacgtt cgtaggtaac tgattgttgc
     2941 cgttcttttc gttcctgttc actagttgaa aaagggattg tatcactgaa taaattataa
     3001 caagcttctt ctgtctgcgt atctttaaaa tattgcacgt cttttttggt tcgctcatag
     3061 ttgtcttgaa cctctttttg ctgtttttct ggcaagtttt gataaagacg agtgagtttt
     3121 tccatgtttt tatccgtcaa acgtttttct tgattcacaa aaatatcgtt catttcctca
     3181 taacaagtgg taaactcttc cattttttgt gttaacactt gctccttttt ctttttatca
     3241 catgctgctt catacttttg agtgatatcc cctaataaaa tacttcttga aatacgttta
     3301 tttccctcta aaacgccgaa taaaccactt aaaacgattg ataatgtaaa tataacccct
     3361 ggcaataaaa acctcttaaa tcgaatttta gagcctttta atgaatatct tgtttgataa
     3421 gccttatagc taagccaaat aattcctaaa atactggcaa aaatgagtag atagggtaaa
     3481 tagaatgcca tttctattta ttctccccat accagccaaa aggctaacag tgtcatgatt
     3541 aagcaacata agggggcaag aatatccatt gatcgctgca taattacttt tttctgttct
     3601 ttatttcgct ggtttaatag cgcatggtgg aattttttga cttgtgaatc aattgtagtt
     3661 tgtattcttt ctttggtata aggtgcaaga aaagcacgtt gtttctttat ttcattttct
     3721 atgtcttgta acgttttaga acgataattc ttgttggtag tctgctcctc tttcaacgtg
     3781 ttggtcatgg tttcttcgag tcggtctaat ttccggttgt tcagtcgtaa gagttcgttg
     3841 ttctcttcgt acttcttttg cagtttcgcg aatgctagct cgagctcttc gtagctcgtt
     3901 ggttcgttgt tgattgtagg ctcgttctcg tctgtccagc tcatgtaaaa tcgtctcctt
     3961 ttcgtaatcc tttcctagtt tgttggatcg tacttttttc tttttgtgga tcaattcgta
     4021 agtaaaattt ttcccacgat tcaagcaatg aatgccgaat gttttaagag aatcacaaaa
     4081 ttggttatac gttcgagttt ttttgttttt cattgtttga tcaatcgcaa accgaatttc
     4141 gtctttccaa acaggttcat ttttttcttt taatttgatt tcagacatgg ttcgtttttc
     4201 gggtttcttc cctcgttcag tgattgttaa gtcatgttca agacaaatct cgtcatttag
     4261 gtcataacat ttgtttaaaa atgcttgacc atgggcttga aatttgttcc cagtttccag
     4321 attgacggag ttaatgacaa tgtggttatg aatatggtct ttgtctgtat gtgtgtaaat
     4381 cgcgatttgg tggtcagggg ctattttttt cgcatactct gcccctagtt gattggcttt
     4441 ttcagcggta atttctcctg gtcgaaaaga ctggatcaat aagtgtgctt gtacacgatc
     4501 gtcttttcca tacagcatgc gaatttgttt catttcgctt ttggcataat caatgtcaca
     4561 gtttatccct gttttgacgg ttgctcgtgg ttcggcgtaa ttgatcgctc ggctacaaga
     4621 agtggttcgg cttactttaa ctgttgccat atcttttgta gctccttttg catgattttt
     4681 aggttttcgg ataagcgttg ggcgtcgcca ggatcaacgt cgtgttggtg tgtgttgcac
     4741 cacttggcaa tttgattgat attcccgcca gctttggcta aatcgcgcgc gattgcctga
     4801 gcaatgtctg gcgccacttt cggtgtcacc agacgcgcgt tttgtgcctt ggctttcacg
     4861 aaagccggca cactcatttg caaagcttca gctgactgct tcagctttgc aaattcggat
     4921 tcgctcactc gaaaactgat ttgttttggc tcggatcgat aggtatattt tttagaatga
     4981 ttgctagcca aatattgatt ttgttcgctc ataagtccca ccctttcagt acgtcctacg
     5041 gacgtatcgt tcgcaatacc attgtaccta aaaaggtaca ataagtaaag ctcactaaag
     5101 ctcactaaag gg
//
LOCUS       DAZKKW010000134         5085 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.136, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000134 DAZKKW010000000
VERSION     DAZKKW010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5085
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.136"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..366
                     /locus_tag="ACG8PO_002648"
     CDS             <1..366
                     /locus_tag="ACG8PO_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFQ0207908.1"
                     /translation="IHILYGSGLYALMYFIPIFLAIYFGTNLVSQIVDAIPEWLTNGL
                     TVASKILPAYGFALLLQTMLTKKMVPFLLLGFLITAYSGLGIIGIAAFACIVAFVMVQ
                     VQGKNSNGNQNEPDPLDDL"
     gene            380..1192
                     /locus_tag="ACG8PO_002649"
     CDS             380..1192
                     /locus_tag="ACG8PO_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFQ0207909.1"
                     /translation="MKEKDQNAWKYRQFLWRSWAIQASWNYERQMNMGFMYGIAPILD
                     NIYKNPEDEAMKKEAYERHMMYYNCTPQTSAFVLGLTASMEEQYYEDQENFNPESISA
                     VKTSLMGPLSGVGDSFFQGTIRVLAFGLGINLAQQGSILGPILAMLISFVPSFLVTYY
                     GGKIGYNMGNKYLSKLYQEGLMDKVMYVCSIVGLMVIGSMIASMIGITTPIQMGKAFV
                     LQDVLDSIMPQFLPLGLTFFMYWLLQKKVKTGWMLAICLVGGIIFSLLGILQ"
     gene            1246..2211
                     /locus_tag="ACG8PO_002650"
     CDS             1246..2211
                     /locus_tag="ACG8PO_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFQ0207910.1"
                     /translation="MNSKEIVKEIVAKKEVKSIVFVGCGASKADLYPAKYFLDQNAKQ
                     LRISHYTASEFNYATPASVDESTVVISASLGGTTPETVEANAVAKEKGATVISLTRSI
                     DSPLTKDADYVIYHRFAENYAAKLEKTGYCLELAVELLHQVEGYDHYEAMQEGFATIY
                     DLAQKSAESARKDAKKFAERFKDEKVIYVMSSGATHEVAYSTSICLMMEMQWINSGTF
                     HSGEFFHGPFEIVDKDVPFILLMNDGKTRNIDARALTFLERFDTKTEVVDALDYGLSS
                     HIAKEVLDYFNPFVITAVFRVYAEELSEAREHPLTKRRYMWKLTY"
     gene            2327..5050
                     /locus_tag="ACG8PO_002651"
     CDS             2327..5050
                     /locus_tag="ACG8PO_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFQ0207911.1"
                     /translation="MQRLNDYLYQLTLLLKSTPSDNYSARSISQALQLNRSTISSYLN
                     EGVREGLFIKVKSYPVLFLHRTALEELHITLNNSEIESIESLLTISQKPALDQVIGSK
                     GSLKEAIDQIKTAVLYPGKGLPLLLIGASGSGKTFLANKIYEYAVEEKVIHTSAPFID
                     YNCAQYVSNPELLSSALFGYTKGAFTGASQEHTGLLEKADGGVLFLDEVHRLSEEGQE
                     KLFTFMDTGEFSPMGDNSIRKKADVRLVFATTENIYTTFLPTFLRRLPVIVNLPRFQQ
                     RPSFERLSLIDEFFVSESKILAKELSVSDALIHFLMNSDFEGNVGNIKNIIKYACGNA
                     YIHQKSQQTIQVKLLDMPLEYSYKFKEQLNKPRKKRADRIYYPEETRQVQLERNNQLF
                     VQFFDQLLTGFSEVIENNKDIKLFLEEMVTKVTQVMDTLIFQEDYEKEQSLYAILNYH
                     IRQSIDFMRESYGFEQDGNRVVALANYLYVKDNQVIFENSLQEKKTKDQLNEFFANVL
                     ETPYWYAKKILSLLSECLDQPVYEEDIFFITLYFYSLNVTNVKTGIKSIVLAHGYSTA
                     SSMANVANRMLNKNVFQAYDMPLDITLEKIEAKLIQFLGDYRTDSGLILLVDMGSLNQ
                     LGGLLVNHLNGPLLIIDHINTPLLLEVGQQVLNNKSITEIGAEIETSLTFKKQLLLPK
                     KKKKKAIITCCYTGIGSATQIQEILTKCLKDAVRELVIIPYDYKKLLENKNFETPFQL
                     YDVIMIVGTEDPKINQIPYIGLDRLINGEAIGEFVHYLEKELTIEVNDLQKELIFNFS
                     ISKIVENLTILDPDKVLSFVQKAIKELSKLLNFDFSNNQLFLLYLHCSCMIERIVRKE
                     SVDEQEDIEEYMQANGEIMDKIHSAFFEIEREYTIEIPDLELRLLSDIVQG"
BASE COUNT         1738 a          755 c         1004 g         1588 t
ORIGIN      
        1 attcatatac tttatggctc gggattatat gctctcatgt attttattcc tatttttcta
       61 gcaatttatt ttggaacgaa tcttgtttct cagattgttg atgcgattcc tgagtggcta
      121 acaaatggct taacggttgc aagtaaaatt ttaccagcat atgggtttgc gttgttatta
      181 caaacgatgt taacgaaaaa aatggtgcca tttttattac ttggattttt aattactgct
      241 tattctggat tgggaatcat tggtattgca gccttcgcat gtattgttgc gtttgtcatg
      301 gttcaagttc aaggtaaaaa ctcaaatggt aaccaaaatg agccagatcc attagatgac
      361 ttataaggag gtagattaag tgaaagagaa agatcaaaat gcatggaaat atcggcagtt
      421 cttatggcgt tcatgggcaa ttcaagcctc atggaactat gaacgacaaa tgaacatggg
      481 ttttatgtac ggaattgcac caattctaga taacatttac aagaatccag aagatgaagc
      541 aatgaagaaa gaagcttacg agcggcatat gatgtactac aactgtaccc ctcaaacaag
      601 tgcatttgtg ttagggttaa ctgcttctat ggaggagcaa tactacgaag accaagaaaa
      661 ctttaatccg gaatcaatca gtgcagtgaa gacttctctg atgggaccgc tatctggtgt
      721 tggtgattct ttctttcaag gaacgattcg ggtattagcc tttggactag gaattaattt
      781 agcccaacaa ggtagtattt taggtccaat tctagcaatg ttaatttcct ttgtaccttc
      841 ctttttagtt acttactatg gtgggaaaat tggctataac atggggaata aatacttgtc
      901 taaactgtat caagaaggtt tgatggataa agtaatgtat gtttgctcca ttgtggggtt
      961 gatggtcatt ggttcgatga ttgccagcat gatcgggatt acaacaccaa ttcaaatggg
     1021 taaagctttt gtcttacaag atgtattaga tagtattatg ccacaatttt taccattagg
     1081 attaaccttc ttcatgtact ggttattgca gaaaaaggtc aaaacaggat ggatgttggc
     1141 aatctgttta gtcggaggaa ttatttttag tttgttaggt atcttacaat aagggaataa
     1201 ccaataaatt tggttgaata atataaataa ggaggaattc aattgatgaa ttcaaaagaa
     1261 attgttaaag aaatcgtagc gaaaaaagag gtaaaatcaa tcgtatttgt tggctgtggc
     1321 gcttcaaaag cggaccttta tcctgcaaag tattttctag accaaaatgc gaagcagtta
     1381 cggattagtc attacacagc cagcgaattt aactatgcaa caccagcatc tgtagatgag
     1441 tcaactgtcg ttatttcagc ttctttagga ggtacgactc cagaaacagt tgaagctaac
     1501 gcagtagcta aagaaaaagg tgctacagtt attagtctaa cacgaagcat tgactcaccg
     1561 ttaacaaaag atgcagatta tgtaatttat catcgatttg cagagaacta tgctgctaaa
     1621 ttagaaaaaa caggctactg tttagaattg gcagtagaac ttttacatca agtagaaggt
     1681 tatgaccatt atgaagcaat gcaagaagga tttgctacta tctatgatct tgcccaaaaa
     1741 agtgcagaat cagctcgtaa agatgcgaag aaatttgctg aacgctttaa agatgaaaaa
     1801 gttatttatg taatgagtag tggtgctaca catgaagtag catactcaac atcaatttgc
     1861 ttgatgatgg aaatgcaatg gattaattct ggtaccttcc attcaggaga atttttccac
     1921 ggaccattcg aaattgtgga taaagacgta ccgtttatat tattaatgaa tgatggaaaa
     1981 actagaaata tagatgctcg agcattaact ttccttgaac gctttgatac gaaaacagaa
     2041 gttgtagatg ccttggatta tggcttgtct tcacatattg ctaaagaagt cttagactac
     2101 tttaacccat tcgtgattac tgcagtattt agagtttatg cagaagaatt gtcagaagca
     2161 agagaacatc cattaactaa gagacgttat atgtggaaac taacttatta aaaaatagat
     2221 caaactaaat aaggtggctt gagaaattaa gccaccttgt tttcttcaat gtgattttat
     2281 acatatggta aaataactag tacatattgt gaaagcaggt ggcaagatgc agcgattaaa
     2341 tgactatctt tatcaactaa cattgctttt gaaatctacg ccttcagata attattcagc
     2401 tcgcagtatt tcgcaagctt tacagttgaa tagaagtaca atcagtagct acttaaatga
     2461 aggtgttcgt gaagggcttt tcataaaagt aaaaagttac ccggttttat ttttacatag
     2521 aacagctctt gaagaactac atatcacact gaataactcc gaaatagaat caattgaaag
     2581 cttgttaaca atttctcaaa aacccgcatt agatcaagta attggctcta aaggaagttt
     2641 gaaagaagca attgatcaaa ttaaaacggc agtcttatat cctggaaaag ggttgccttt
     2701 attattgatc ggcgctagtg gatcagggaa aacctttcta gcgaataaaa tatatgaata
     2761 tgcagtagaa gaaaaggtca ttcacacatc ggcacctttc atagattata attgtgcaca
     2821 atatgtgagc aatccagagt tactatcctc tgctttattt ggctacacga aaggagcatt
     2881 taccggagca agccaggagc atactggttt gctagaaaaa gccgatggag gagtattgtt
     2941 tttagatgaa gtgcatcgct tatctgaaga agggcaagag aaattattta catttatgga
     3001 tacgggtgaa ttctctccta tgggggataa tagtattcgg aaaaaagccg atgttcgatt
     3061 agtctttgca acaacagaaa atatatatac gactttctta ccaacttttc ttaggagatt
     3121 gccagttatt gttaatctac ctagatttca acaacgtccg tcgtttgaac gtctgtcatt
     3181 gattgatgaa ttttttgttt ctgagagtaa aattttagct aaggaactat ctgtttctga
     3241 tgcattgatt catttcttaa tgaatagtga ttttgaaggt aacgtgggga atattaaaaa
     3301 tattatcaag tatgcttgcg gaaacgcata tatccaccag aagagtcagc aaacaattca
     3361 agttaaatta ttggatatgc ctttagagta cagttataaa ttcaaggagc aactaaataa
     3421 acccagaaag aaaagagcag atcgaatcta ctatccagaa gaaacacgac aagtgcaact
     3481 agaaagaaac aatcagcttt ttgttcaatt ttttgaccaa ttacttactg gattctctga
     3541 agtgattgaa aataacaagg atattaaact atttttagaa gaaatggtta ccaaggtaac
     3601 acaagtaatg gatacgctta tttttcaaga ggattacgaa aaagagcagt ctttatacgc
     3661 cattctaaac tatcatattc gtcaaagtat tgattttatg cgagaaagct acgggtttga
     3721 acaagatggt aaccgggtag tcgctttggc caactatcta tatgtcaagg ataatcaagt
     3781 gatttttgaa aatagtcttc aagaaaagaa aaccaaagat cagcttaacg aattttttgc
     3841 taatgttctt gaaacccctt attggtatgc aaaaaaaatc ttaagccttt tatctgagtg
     3901 tttagatcaa cctgtgtatg aagaggacat tttctttatt acgctatatt tttatagttt
     3961 gaatgtcacg aatgttaaaa caggtattaa gagtattgtg ctagctcatg gttattctac
     4021 agctagtagt atggcgaatg ttgccaaccg tatgctgaat aagaacgttt ttcaagctta
     4081 tgatatgcct ttagatatta cattagagaa aattgaagca aaattgattc aattccttgg
     4141 agattatcga acagattcag gcttgatttt attggtagat atgggttcac taaaccaact
     4201 aggcggttta ttagtgaatc atctaaatgg accactgtta atcattgatc atatcaatac
     4261 accgttatta ttggaagtcg gccaacaagt attaaacaat aaaagtatca ccgaaatagg
     4321 agcagaaatt gaaacaagtc ttactttcaa aaagcagtta ctacttccca agaaaaagaa
     4381 aaagaaagcc atcattactt gttgttatac agggattggt agtgcgaccc agattcagga
     4441 gattttaacg aaatgcttga aagatgcagt tagagaatta gtaattattc cgtatgatta
     4501 caaaaagctg ttagaaaaca agaattttga gactcctttt caattatacg atgtcatcat
     4561 gattgtaggt acagaagatc ccaaaattaa tcagattcca tatattggat tagatcgcct
     4621 gattaatggg gaagcaattg gcgaatttgt tcattacttg gaaaaagagc tgactattga
     4681 agtgaacgat ttacaaaaag aattaatttt caatttttca attagtaaaa ttgtcgaaaa
     4741 tttaaccatt ttggatccag ataaggtgct atcttttgta caaaaagcaa ttaaagaatt
     4801 atcgaagtta ttaaattttg atttttctaa taatcagctg tttttgttgt accttcactg
     4861 tagttgtatg attgaacgaa ttgtacgtaa agaaagtgtg gatgagcaag aggacattga
     4921 agaatatatg caagcaaatg gagaaataat ggataaaatt catagcgctt tttttgagat
     4981 tgaaagggaa tacacaattg aaataccaga tttagagttg cgattattga gcgatattgt
     5041 tcaaggatag gagtgaattc gaatgacaat gggagtaatt tttga
//
LOCUS       DAZKKW010000135         4928 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.137, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000135 DAZKKW010000000
VERSION     DAZKKW010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4928
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.137"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(220..972)
                     /locus_tag="ACG8PO_002652"
     CDS             complement(220..972)
                     /locus_tag="ACG8PO_002652"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFQ0207912.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            1085..2005
                     /locus_tag="ACG8PO_002653"
     CDS             1085..2005
                     /locus_tag="ACG8PO_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFQ0207913.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            2078..3013
                     /gene="murB"
                     /locus_tag="ACG8PO_002654"
     CDS             2078..3013
                     /gene="murB"
                     /locus_tag="ACG8PO_002654"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFQ0207914.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            3311..4789
                     /locus_tag="ACG8PO_002655"
     CDS             3311..4789
                     /locus_tag="ACG8PO_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0207915.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
BASE COUNT         1739 a          787 c          943 g         1459 t
ORIGIN      
        1 atctgcggta ataagcccaa ccaaacgaaa aatatgagga actatttttg tttggctgtt
       61 cttattactt gatgcaggac agctttttca caatctctcg ataaacagtg ttcaaaagcc
      121 agtttctcgg tattatgcca aatttatgca aaatgtggaa agccattttc acaaaatttt
      181 tcttaatact cgaaactaaa cgactttttc acaacctctt tatttcaaat ctaattctac
      241 tggacaatga tcactgccca taatgtccgt atggatcttt gcatcagcta gtcctggctc
      301 taaatctttg ctcgtcaaaa agtaatcgat ccgccaacca gcattgtttt tccttgaatt
      361 gaatcggtag ttccaccaag agtagacacc ttcctgggtt ggataaaaat aacggaatgt
      421 atcgataaac ccgttatcca acagtcgaga aagagccgct ctttcttctg gagtgaatcc
      481 agcatttttt tgattcgttt tccagttttt caaatcaatc ttttgatgag cgacatttag
      541 atccccacac acgatgactg gtttttgttt ttttagattt tccaaataat tataaaaggc
      601 ttcttcccat tctaatcggt aatctaaacg ttttaattcg ctttgagaat ttggcgtgta
      661 acaggttaca agaaagaaat cggaatattc caaagtaatc agacggcctt ctgtatcatg
      721 gatatcgata cccattccgt aagtggcatt caatgctggt tttttagcaa aaatcgctgt
      781 gccggaatag ccttttcgtt ccgcgtaatt ccaatattga taataacctg gaagatctaa
      841 atcaatttgt cctgcttgga gtttcgtttc ctgcagacag aaaaaatcgg cgtctaactc
      901 atgaaaaact tctaaaaaat ttttatttac gatggcacga agcccgttta cgttccaaga
      961 gataaatttc aataaaagca cactccttcg aattaatttc cttacctttg cgatactatt
     1021 gtacctcatt ttagtaaaaa atggtgtaag atgaaagtaa ctaaatgatc caggaggaaa
     1081 gtagatgaag attggtgtaa tcggtgtagg gaatatcgca gaaaaagcat atttaccaac
     1141 ttatgcgaaa aaacaaggaa cacttgattt ttattttgca acaagaaata aagaaacaaa
     1201 agaaagaatt caaaaagagt acgggttcca acatctatat gaaacgatcg atgagctttt
     1261 agaagaaaaa atcgaagcat gtatgatcca cgcagcaaca aaagtacatt atgaattagc
     1321 caaaaaatgt ctagaaaatg gtatccatgt ttatattgac aaaccactga gtgtccatct
     1381 aaatgaagtt cgtgaattgc aagaattagc tgataagaac caaataattt tgatgatcgg
     1441 tttcaaccgg cgctttgctc ctatggtaga agaattgaaa cgtatcccag ataagcggtt
     1501 gatccacctg caaaaaaatc gaatagcggc aaaagaaacg acagagttcg tcctttatga
     1561 tttgttttta catcttattg atacagctgt gtatttatta gatgaaccca ttttgagaac
     1621 aaaataccag ataagagaaa caaaggaatt tttggaatat gccattttac aattagaaac
     1681 acaaaatcaa acggctattt taacgatgga tttagcaagt ggcgctaata tagaaaaata
     1741 tcaagtaaca agtaaaaatg gtacttatga agtaaacgat ctgacccagc ttgtcattca
     1801 agatgctgat ggaaaaaaag tgaaagaatt tggcgactgg acaaacacgc tagtcaaacg
     1861 cgggtttgag ccaatggtag aggccttctt ttctcagatc cgtttaggta aatcggaaac
     1921 agaattgctt aaacaaaaag aagttgtaag aagtcatgaa ttatgtgagg aagtgcttaa
     1981 agatcactac cgtcatcaac tttgaacaag actggcaaga ttgtattaag cgtgatagaa
     2041 taatgaggac gtttgagaaa agaaaggaaa atcctcagtg aataaagaag aaatagttga
     2101 aagcttagcc acgattacta tattgtttga tgaaccattg atgaactata cgtttacgaa
     2161 aacaggtgga ccagctgatg tccttgcatt cccgaaaaaa caagaagaag taaagcaaat
     2221 catcgattat tgccgaatcc atgatatacc ttggatggta ttaggaaatg caagcaatct
     2281 gatcgttcaa gatggcggga ttcgcggtgt agtcatcatg ctgactgaaa tgaaacagat
     2341 tcatgtcaaa ggaacgatgg tcattgcgga agcaggtgcc tcactgattg atacgactta
     2401 tgcagctttg gcagaatcat tgactggatt tgaatttgct tgcggcatcc cgggaagtgt
     2461 cggtggagcg gtttatatga atgcaggtgc gtatggcgga gagatcaaag atgtttttgc
     2521 tgaagtggat cttttgcttg aagatggaac attgaaaact ttgacaaaag acgaaatggt
     2581 attttcttat cgtcacagta aagtccaaga attacgtgct atcgtattgg aagctcgatt
     2641 cagtttgcaa acaggggatt atgaagcaat caaagcaaga atggatgagt tgaccgaatt
     2701 gcgtcaatca aaacagccat tagaataccc ttcatgcgga agtgtcttta agcgcccagt
     2761 aggacactat acaggtcagc tgatccaaca agcaggattg caaggcctga aatggggcgg
     2821 tgcacaagtc tctgaaaaac atgctggatt catcgtcaat atcgatcatg cgacagcgac
     2881 agattatatt gaattgattg cgcacatcca agaagtaatc aaagaaaaat ttgatgtgtc
     2941 tttagaaaca gaagtgcgga tcattggaga aaaacaagca atatcagaaa caagcagaag
     3001 tttagccaat taataagtaa tggaacttaa aataagtaaa agagcaggac ggtggccgtc
     3061 ctgctctttt ttgtggttgt ttacgcaaat ggcgcaaatc ttgtacattt taacacaata
     3121 aaacaaaaaa tatagaatat ttcctcataa atttttccat tttttgcaat attctcaaaa
     3181 aaaagataaa aaatgagaat aactgatgaa aaaacaagca taatccatca taaaatcagt
     3241 ataggtgaaa agatgaactt tcatctgcat atcaagtaat tggtatgagg cattttttga
     3301 ggtgtaggat atgaagttgg attaccttat agaagaggaa gatagagaaa aactgctgct
     3361 agctagatat ttagaatttg agaaaaagaa ttatgtagtg ctgcctaagt tatatgaaac
     3421 attaggtttg tctaaattta aaacaagaaa ttatatcata gaattaaata aagaatttac
     3481 tgaattcaaa acaaagccag agatccaaat ttcagaagaa gcagagatcc atgtcgaacg
     3541 tctgaatttg acggatatca aaaatttaag aagaatctac tttcaacgat caaaaattgc
     3601 tcgactgtta acggagatcg tggattcaaa agtaacgatc gaaaaatttg cagagaatca
     3661 ttttttaagc atatcaagag cctatgtaaa aagaagagaa ttagtctctt ttcttgaaca
     3721 gcatcaaata aagttaaaga aaaacatatt agttggggaa gaaataaaca tacggaacgt
     3781 attatttggg gtatatttcg gggtctttaa tggtttttct atgccattta gagaaagcgt
     3841 catgaatgaa gcaaagagta tcgtgcaata ttttgaatat ctgttcaatt taaaactgtc
     3901 tgcaacaaaa cgaatcaaat tcaatttgct aaccactatt agtttgttga gggcctatgg
     3961 gaatcaacca atcggaaaac gtttcttttc agatgaatac tttaagagct cagaaggaat
     4021 gaagatcatc aaggatttat cacatatttc ttttcttcat atccctaaag agcagatcat
     4081 tgatgagatt tcttatatca tgttgttttt gatcacggaa gaagaatttg aattagatgg
     4141 cgcatttgga cttgcggaag ctcattttca tgatatccac atgattagtc aaaaaatgtc
     4201 agtggaattg attgatcaat taccattttc gattgaaatc tctgaagaag aaagactata
     4261 tatacgaaaa gaacttcgta tgggtatgat gcatgtcaat cgaaaaaatt ggatattcga
     4321 ctttgagatg actagcttta cgtctaaaaa gcagctacag tttttcttcg agacgtatcc
     4381 aatattcagt acagcaatcc aaaaggtcat acaagagaat aaacgctatt tcttgggaaa
     4441 agatgaacgt ttcatgattc gcttatttta tgattatctc tttttattgg tcggtatttg
     4501 tccggtatca tattttgaag caccgattca tgtctgtatt gatttttcgc aaggatctgc
     4561 ttataccgat tacattattt ctcaagtgaa aggatttaaa aattacaata ttgtgattga
     4621 gagtcgaatc acttctcgta cgaatattta tttatctgat tgtttacttg aaaaatttaa
     4681 taaagaacag ataatttgga aaaatccacc gactccagat gactgggaat attttggaaa
     4741 taccatcatc agagaaaaaa atagacattt aactgaaaaa atcagctagt atttatggat
     4801 ggcagctttg tctcacatat ttattagctg atttatgttt ttttcaaaga aaaggtaact
     4861 agatataact agatataact agatatatta cttaacaaaa ttaaaaagta cataagttat
     4921 tattccga
//
LOCUS       DAZKKW010000136         4869 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.138, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000136 DAZKKW010000000
VERSION     DAZKKW010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4869
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.138"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(3..1151)
                     /gene="nagA"
                     /locus_tag="ACG8PO_002656"
     CDS             complement(3..1151)
                     /gene="nagA"
                     /locus_tag="ACG8PO_002656"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFQ0207916.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(1227..2063)
                     /locus_tag="ACG8PO_002657"
     CDS             complement(1227..2063)
                     /locus_tag="ACG8PO_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0207917.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(2247..3464)
                     /locus_tag="ACG8PO_002658"
     CDS             complement(2247..3464)
                     /locus_tag="ACG8PO_002658"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFQ0207918.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            complement(3613..3774)
                     /locus_tag="ACG8PO_002659"
     CDS             complement(3613..3774)
                     /locus_tag="ACG8PO_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0207919.1"
                     /translation="MEKYDGEFSGLGMILGVLIGLAFGRFLLGFMLGIICGIAMDWAA
                     NLWNDYHDN"
     gene            3847..3930
                     /locus_tag="ACG8PO_002660"
     tRNA            3847..3930
                     /locus_tag="ACG8PO_002660"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3881..3883,aa:Leu,seq:gag)
     gene            complement(4170..>4869)
                     /locus_tag="ACG8PO_002661"
     CDS             complement(4170..>4869)
                     /locus_tag="ACG8PO_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0207920.1"
                     /translation="GNALRAAHEINKWFPEYKNFNFLNKLDLGISSFGKPFFLDMNIL
                     FLLLFFLIFLLTYRKCQNYYVRILTALPFFLNLIIYFGNTMGQSFTYVNGNKRAMIWS
                     SSNLNNLFTELGTKLSLFYPGTWIATLVVLALLLCLIVGIYLSFDNKKTSIFLVILMI
                     MGFCSRLIMGFSPTVWASGMRTYYILYVVIAILVLMAVKELMKSMSVQKNEFMQFGLT
                     VLGICTFIITVINR"
BASE COUNT         1481 a         1014 c          783 g         1591 t
ORIGIN      
        1 tattatgcgt ggaaacgttc cacgccatct agataagttg cttccaatgt catatcaggg
       61 ttcaatacga taaagtctgc atcgcgtcct tgagcgattt ttccacatgt atcatcgatt
      121 ttacagctga ttgctggaat cagactagcc atcatgactg cttgttctgg tgttgctaat
      181 ccccagtcta caacattttt aattgcttct ttcaatttca atatacttcc agctaagttg
      241 ccagattcta gacgtgctgt tccttctttt actactactg gaaattcgcc taaattgtaa
      301 tttccatcag gcatcccacc ggccatcata cagtctgtaa ttaatgcgac atgatcatgt
      361 cctgcttttt ccattaggat tcccgcagca tttggatgta catgatggcc atcacaaatc
      421 aattcagaaa agacatgttc taaagataat aacgcaccca ccatacctgg ttcgcgatga
      481 ttcaatccac gcataccatt gaaagcatgg acaaatacac ttgcgcctgc ttcaacagct
      541 ttttgcgctt cttctagtgt agcattactg tgtcctagag aaacaactac tccttcttca
      601 gtcacttgtt tgacaaactc ttctacacct tgacgttccg gtgctaaagc aatttttttg
      661 attaaaccac ctgaagcttc ttgccattca tggaaagtat ccaaatcagg atccccgaaa
      721 taacttgggt tttgtgcccc tttgtgttct tctgtgaaga aaggaccttc aaaatagatt
      781 cctttgattt tagctcctgg tgcttcctga tacattttgc cgattgtctc agctacatct
      841 tttaaacgtt ctttgcttga cgtcaatgtt gttggtaaaa atgaagtcac tccacaagat
      901 aacagacctt cagacatcac tttgatgcct tctgcatcat tatccattac atcatgattc
      961 atatagccgt gaatatgtgt atctaccaaa ccaggagcaa tccatttgcc gctttggtca
     1021 atgatgttga cttctccctc aggtagttct ttggtgtatt caccaaaaag tccatcggta
     1081 atttcaagat agcctgcatg tttgacacct gacgctaaaa agaatttgtc agcatagata
     1141 aaagttctca ttctattctc tccctttgtt tttctacctc taaagtactc ttatttagag
     1201 aagaagtcaa gaatggtcta caccatttac aaattttttg aaatttgttt cacagcttgt
     1261 gctaagcctt catagaatgc tgctctttct ggttcttctt tacgcttgta tattaagctt
     1321 ttaagcacaa ataaatcatt gaggtaatag tagctattct tctcttgagc ccaatagatt
     1381 ccttgattca cctgttctaa cgcttcgtcg atttcatttt gatggaataa gaaatttcca
     1441 taattataaa aaatttgcga aaattcactt tttgatcgtt catcagctga ttgatgcagc
     1501 agttggatgc ttcgactcaa atagtagcgt gcttcagccg tttttcctgc cacaccatat
     1561 atttttccga tacaactaat cagttgtatt tctgtgtcag aaacatggat tctttctttt
     1621 tggctagagt agcttaatgc ttctttcaaa taataaaggg actgagagat attttgacgc
     1681 aacgtgaatt cgcaaacacc gagataataa taatagcgtt gaaagtcagt tgcttgatac
     1741 agattttcag aaatatcaga agattttagt acttgttcta acttccgata atcacgtttt
     1801 tggaaacaac tatccaacag ttcaaataat ttatcgtttg tccgagtagt ttttcgagag
     1861 agattcatca catgatcaat cgttacccct agccgttgac aaatctgcat catcaccaat
     1921 acattcggca gttcctcatt attttcgatc cgacttaata cactttgtga acaaatatca
     1981 tgcgaaagca ttttttgcgt cattttttgt tcttttcgaa tctctttaat gatcgttcca
     2041 aaggactcga ttaacgtttc cataagtatc ctccccaaaa tatgcgtatt cgcattctat
     2101 tcgtatttta ctttaggaag tcgcaaactt caagaaaaga cttgcaacta aataatatac
     2161 tttagttatt taagaaaact ccactagaaa gagagcctca gtgtgtttgt ctgtggccct
     2221 cctactgaaa aatttggttt ttaaaatcat ttttggcgat aggttgctag gaattctctc
     2281 attttcttag ctgtttgttt caaatcatcc attttaggaa gataaacgat tcgaaaatga
     2341 tctggctggt tccaattaaa tccaccacca tgtacaagta gaatatgatg ttcatgtaaa
     2401 aagtccaaaa cgaatttttc atcatccaaa atattgaatc gctttgtatc aatttttggg
     2461 aatatataaa aagcagcttt tggttttacg gctgaaagac caggaatatc attgattgca
     2521 ttatagatat attctctttg ttcatagatc cttccgcctg gcaggagcag ctcatcgaca
     2581 ctttgataac cgcctagcgc ggtttggata atttgctgag acaatacatt ggaacacaaa
     2641 cgcatggagg acaacatatt cagcccttct atatagcctt tgacgtgtga cttatctcca
     2701 cttaggacca tccagcctac tcgaaatcct gctacacgat gagatttgga tagaccgttc
     2761 agcgtaacaa cgaacaaatc aggcgctaat gtagcaatcg gaatatgttc tagaccatcc
     2821 atcactaagc gatcatagat ttcgtcggaa taaataatca ggttattttg tcgagctact
     2881 tcaacgattt cttctaataa ttcttttgga taaagtgccc cagtaggatt attaggatta
     2941 atgataacaa tagcttttgt ccgactcgtt atttttgatt tgatatcatc aatatcagga
     3001 taccattctg cctcttcatc gcaaatataa tgaacaggtg tgcctccagc taatgcaata
     3061 gaagccgtcc acaacggata gtccggcatt ggaaccagta cttcatctcc attgtccagc
     3121 aacccctgca tacacatcgt gatcaattcg ctcactccgt tgccggtgta gatatcattg
     3181 atcgtcacat tcggaaaatg tttcagttgg caatattgct caattgcttt tctagcagaa
     3241 aagatcccct tggaatctga gtagccttct gagtcacggg cattcatgat caaatcacga
     3301 atgacttcgt ttggtgcttc aaatccaaaa ggtgcgggat ttcccgtgtt caattttaaa
     3361 atactaatgc cctcttcttg catccgctcc gcttcttcta gaacgggacc gcgaacatcg
     3421 taactgacac cttctaattt gttggatttt tcaaattctc tcatcactta gccaatccct
     3481 catttcaaaa agttagaact ttccaaattt tttgttattt actatttcaa tatcatactc
     3541 catcctagaa aattttgcca cttatttagg aaaaacgtaa gtattaaaaa aacacaaaca
     3601 agcgttgaga acttaattgt catgatagtc attccataga ttcgctgccc aatccattgc
     3661 aattccacaa atgattccta gcataaaacc aagcaaaaaa cgtccgaatg caagtccaat
     3721 caatacacct aagatcattc ccagtcctga aaattctccg tcatatttct ccatcttaat
     3781 aagtcctccc atttttgagc ttgacaacta tgtaaaacct cgctatcatt agtgtgttat
     3841 tattacgcag atgtgttgga attggcagac aagcatgatt gagggtcatg tgagcgttag
     3901 ctcgtgtggg ttcgagtccc atcatctgca ctacaactcg tgaagttaat tttgcttcac
     3961 gagttttttt gtttgaacgt ttctttatgc cattatatac atatcactgt atcctttaca
     4021 aaaagaacgt acaatcatgt aaacagcctg aggtgagtat atgaaggtcg gaaacaagct
     4081 actcgtagaa acttgcaagt gttaaaacaa ttaatcgaaa aacgagtaaa atccctcacc
     4141 taatactaaa gcgagggatt ttttgtatat tatctgttta tcaccgttat tataaaggta
     4201 caaataccaa gcacggttaa tccaaattgc ataaattcat ttttttgtac actcatgctt
     4261 ttcattagct ctttaactgc cattagtact aagatggcga taacaacata aagtatatag
     4321 tacgtccgca ttccggaagc ccatactgtg ggagaaaatc ccataattaa ccttgaacaa
     4381 aatcccatta tcatcaaaat aactaaaaaa atagaagttt tcttattgtc aaaacttaaa
     4441 taaattccaa caattaaaca tagtaataat gccaagacta ctaaagtagc tatccaagta
     4501 cctggataaa aaagtgacag tttcgttcct aactctgtaa agagattatt caggttacta
     4561 ctactccaaa tcattgccct tttatttcca ttgacgtaag taaagctttg acccattgta
     4621 ttaccaaaat aaataatcag gtttaagaaa aacggtaatg cagtcaatat acgcacataa
     4681 tagttttgac atttcctata tgttagaagg aaaattaaga aaaatagcaa taaaaataag
     4741 atattcatat ctaaaaaaaa cggctttcca aatgatgaaa tacctaaatc taacttatta
     4801 agaaagttga aatttttata ttcaggaaac catttattaa tttcatgtgc agcacgtaat
     4861 gcattccct
//
LOCUS       DAZKKW010000137         4814 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.139, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000137 DAZKKW010000000
VERSION     DAZKKW010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4814
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.139"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(145..459)
                     /locus_tag="ACG8PO_002662"
                     /pseudo
     CDS             complement(145..459)
                     /locus_tag="ACG8PO_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331484.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            745..984
                     /locus_tag="ACG8PO_002663"
     CDS             745..984
                     /locus_tag="ACG8PO_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0207921.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
     gene            complement(1346..1606)
                     /locus_tag="ACG8PO_002664"
     CDS             complement(1346..1606)
                     /locus_tag="ACG8PO_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0207922.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVIGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(1791..2288)
                     /locus_tag="ACG8PO_002665"
     CDS             complement(1791..2288)
                     /locus_tag="ACG8PO_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0033745 - L-methionine-(R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HFQ0207923.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
     gene            complement(2418..3128)
                     /gene="budA"
                     /locus_tag="ACG8PO_002666"
     CDS             complement(2418..3128)
                     /gene="budA"
                     /locus_tag="ACG8PO_002666"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047605 - acetolactate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0045151 - acetoin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HFQ0207924.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            complement(3141..4805)
                     /gene="alsS"
                     /locus_tag="ACG8PO_002667"
     CDS             complement(3141..4805)
                     /gene="alsS"
                     /locus_tag="ACG8PO_002667"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0034077 - butanediol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HFQ0207925.1"
                     /translation="MAEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVEKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAKLTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
BASE COUNT         1452 a          991 c          742 g         1629 t
ORIGIN      
        1 tataaactct agagaaatta attcatccta acataaaata attgttcact caaaagtttt
       61 tctagaacaa ttaaaacagc aggcttcaca ttcgctatac atttgtaaga agtaataatt
      121 cctgtactaa aaggcataat tgctttaatt attttttgac ttctcttcaa acacaagaag
      181 tagttgtgta ataattgcag aaataaggat tcaaataata gatataaaat ttgacatgga
      241 aacgtctata acattaaaag aataaagtgt tgaagaaagt aaactgattg aaataacccc
      301 tccgtaaaaa atagtctgtg aatataccca ctttttttca gaagacgtac ttcgtttggt
      361 tctaaaacct atccctgcat ttctactctt atcaaaaaac caaatattta gaagtaataa
      421 tattgctatt attaagataa aattactgta aaaatccaaa agctatcctc ctagcaacta
      481 tttaaatacc caaaaaaatt acctctagat aatatcatac ctacgttttc acttataaat
      541 tttttcataa aaaaagcgtt tagaggcata atcgttaata cagatggtaa ccaatgtatc
      601 tggtttgaac tgggatatca ccctctccat atatgtatta ctaagactaa tagatactat
      661 aaaagacttg atagttttgc attgattttg cctaatactg agcataggtg tatgctaaac
      721 gatactataa ggagtgtgta cataatgaaa agaattctgt ttatcagttt atcaaattta
      781 ttgttactta ctgcttgtgg gacttctgca tcaaagaaca taccttcaga tacgcaaatt
      841 tcgaatagta tcgattcaac aatttctaca aatgagaaaa attctccggt agaaacttcc
      901 gatataaaag tttctacctc tgcaaaaaac agggtaaaga aactgtcagc acgtctcctt
      961 tatttcaaac aaactctgaa ttagcttctt catcttcttc agcaaataat gatgatagtt
     1021 tcgcgtttac tcctaactaa atgcaacctt tacaaatgct ttacgataac aagcctgagc
     1081 taaatgatcc tgatatcagt tttgcatttt ttgaaatgat tggatcggat gatcttttta
     1141 aaactactag tttgtctatc cgaaaatagg acagttctgg cagtataggt ttttatcgag
     1201 tttctccacc aggcctcgtt acagagacag attctctagg cgaacctttt gaataaatca
     1261 aaaatcatga acaatcactg ccaactaaat aaagtgcctt caaaagcttg gtcttctcgc
     1321 ttttgaaggc actttattta aatctttatg aaactaaagt atgttcatat ttgttgctat
     1381 agttatatag atattctgtt gtttccaact cataaacgtg agtatatttt gccactactt
     1441 ttccgcattt ttctaatatt tcattgcgat cgacgtcatg atattcgtct acgcagatca
     1501 caacacaatt ttctaattta ctgattactt ctccaatcac tttttgtttc aagccaatag
     1561 gttggcaaat atattttttt cctaaaatga tagtttcgtt attcatattt catccatcct
     1621 taatgttttt tcattattta aaatgataat aacattaaaa tatgattatg aaaatcaata
     1681 acacttgtga ttttataaaa aaataaaaaa accgaaaaac agaaatgccc actgtctttc
     1741 cggctctttt tattcttgat ttctcttctt ttttgtttca atagacgatt ttagttatta
     1801 ataaatgaaa aagcatttat attaaaattt gctaataaaa gatctacata tttttctaga
     1861 aatgcttgat cgatttcatc ataccctttc gttactccgc aatcgatatc taaaacaccg
     1921 attaaattac cttcttttac cattggcact acgatttctg attgtgcggc tgaatcgcag
     1981 gaaatgtaat ttgcatgggt tttgacatca tcaacgatca atgtttcttg tttttcagct
     2041 gattctccac aaactccttt tcccatcttg atacgtgtgc aagaaactcg cccttggaac
     2101 ggtcctaaaa tcaattcatt accatcgaat aaataatatc ctgcaaagac agtctcaggc
     2161 aaagtctcat tcaacaatgc agatgaattt gctaaatttg ctaaccagtt atcttctgct
     2221 tctaataatc cttgttgctg taataataaa atttcgtatg catctttttt ctgtttttct
     2281 gtccacatga taaaaaccgc tcctttttga tctatggaga agattatagc tcagtttttt
     2341 tgtaaacgct aggttttctc ctcaaaaaaa cgagatacgt caggttaatc accttgaacg
     2401 catctcgctg tctttttcta ttctgcttgt tggatttctg ctgctaaatc agcataatca
     2461 atcgtttcgt gaacaaaagt cggatcatca actggaaaat gctgtacaag ggacgagatg
     2521 cggctaatct caacttttcc ttcagccact tcaaaatcca ttacatgtcc gccaaatgta
     2581 tgtgtatcat cgataaaatg caggtgaaat cccgctgcag caactccttg gaataattca
     2641 ggcgtaaaga acccgactaa tgttccttgg acattgcttt gttggaattc tggttgagac
     2701 tctgaaattt ttgctagtct tctgtaaggt ttttcttgct tcggcatgat acggatgtgc
     2761 atatttttga ataatccagt tatcttcact gccaaaaata ggttttcccc ggcttctctt
     2821 actaatatgt cttttttcaa ttgttctcca tctgcagttt ttgagacaga aaaactagta
     2881 tctggcgtaa aatctgtaat tgctgcatag ggggtaagtt cacttccatc taattgggca
     2941 actgagccat ctgatcttcc ttgataagct acgccatcta aaaagatgac ttcaccatca
     3001 agcccatgca atgttcccaa tcccagagtt cctttttcta atatatgagc aatcgtttct
     3061 gttccatcca tcaagccggc catcaaagct cctaatgtcc cgtgctggta taagattttt
     3121 tcagtcatgt cgacacttcc ttaatagaat tgatctggta agattgtttc tcccaatttc
     3181 tcattatcac ggtaatcaat cggaatatca ataattacgg gtccttctgt cgcaaatcct
     3241 tcttctaaag ctttttctaa ttcctctaca cttgtagctc ggattccttt agcgccaaaa
     3301 gcttctgcat atttcacaaa atcaactgga ccaaagtcta cacctgacgc ccgctgatat
     3361 ttcatttttt cttggaactc taccatattg tagtgtccat cattccaaat caaatgaacg
     3421 atattcaatt ttttacgtac cgccgtttct aaatcctggg cagagaataa aaatccccca
     3481 tcgccggaaa ctgatacgat ctgtgtattt ggacgcacca atgcagcaga aatcgcccat
     3541 ggcaatgcaa caccaagcgt ttgcatacca ttactgaata acaaatgacg aggttcataa
     3601 cttctaaagt ggcgtgccat ccaaatataa tgactaccta catctacggt aacagtcata
     3661 tcatctgtta cttttgactg aagcgtgtta ataacttcta aaggatgcaa gatacctgct
     3721 tctccttttg attgaacaat gtcccgctcc gttaattttg cttgaagaga tgctaaatat
     3781 tcttttgcat cttctgacac ttgttgaggc tctaatgaac ctgtcaataa atcaagagtg
     3841 gctgagatat caccgatcaa ttctctttca ggctgcataa aaggatcgat ttcagctggc
     3901 gcttcatcga tcacaatgat ccgcgcatct ttttccgcat tccagtttct tgcttcatat
     3961 tcgattggat catagccaat tgcaataact agatcacttc gtttcaaaag catatctcct
     4021 ggttggtttc taaataaacc cacacgacca aagaaatgcg cttctaattc acgtgagatc
     4081 actccagctg cttggaatgt ctctacaaca ggcaatgctg tcttttcaac taattgcctg
     4141 atggctaacg tttctttttc acttgatcct cgcataccta ctaatagtac cggtagtttt
     4201 gcttctctaa tgcgttcagc taggtaacgg atatcatctg cagaagcaga acctaattta
     4261 ggatcgctca atggcttgat gacattacct tggacaggtg catccactac atcttgcggg
     4321 atactgataa agctcgcgcc tttttttgaa ctcttagcca tacgataagc attggcaatc
     4381 acttctgaaa ttgtttctgg atcttggatt tctgcactgt attttgtgat tggctggaac
     4441 agtgctgcat tatccatgct ttgatgggtc aacttcaata gatcgcttcg ttttacttgt
     4501 ccggcaattg ctaaaactgg gtcaccttct gctgtcgctg ttaccaaacc tgtagctaag
     4561 ttactagcac caggaccact tgttgcgata actactcctg gctctcctgt aatacgtccg
     4621 attgcttgtg ccataaatgc agcattttgt tcgtgacgag tcacgattaa ttcaggacct
     4681 tgatcctcta attcattaaa tacaccatcg atttttgcac ctggtatacc gaatacataa
     4741 tcaacatgat gattaatcaa actttctact acagatttcg aaccttgttt cattttttca
     4801 gccattgaac atcg
//
LOCUS       DAZKKW010000138         4729 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.140, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000138 DAZKKW010000000
VERSION     DAZKKW010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4729
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.140"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(52..522)
                     /locus_tag="ACG8PO_002668"
     CDS             complement(52..522)
                     /locus_tag="ACG8PO_002668"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HFQ0207926.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
     gene            complement(681..1139)
                     /locus_tag="ACG8PO_002669"
     CDS             complement(681..1139)
                     /locus_tag="ACG8PO_002669"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0043768 - S-ribosylhomocysteine lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFQ0207927.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            complement(1310..2455)
                     /locus_tag="ACG8PO_002670"
     CDS             complement(1310..2455)
                     /locus_tag="ACG8PO_002670"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HFQ0207928.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            complement(2468..3385)
                     /locus_tag="ACG8PO_002671"
     CDS             complement(2468..3385)
                     /locus_tag="ACG8PO_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent cysteine synthase family protein"
                     /protein_id="HFQ0207929.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIYL"
     gene            3813..4376
                     /gene="efp"
                     /locus_tag="ACG8PO_002672"
     CDS             3813..4376
                     /gene="efp"
                     /locus_tag="ACG8PO_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFQ0207930.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
     gene            4477..4647
                     /locus_tag="ACG8PO_002673"
     CDS             4477..4647
                     /locus_tag="ACG8PO_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0207931.1"
                     /translation="MKNKKVFDFIYILKDEWQKLEKEKTALIYEEIEMIHKIENIRTI
                     PNYIEAKIFFHH"
BASE COUNT         1478 a          854 c          887 g         1510 t
ORIGIN      
        1 aaggcaaaaa gatgaactag aaatagttca tcttttttta tttttattaa gttaaagaac
       61 ggattcaatt gcagcggtca ttttcaatgg agaagtttgt ggtgcaaatc gttcgattac
      121 tttgccattt ctatcaatca aaaatttagt aaaattccat tttattgctt tagtgacagt
      181 accaggagct tcatttttaa gataagtaaa aagaggatct gtattttctc catttacgtc
      241 aatcaattga tgcataggga aggtaacgcc ataagtggtt ttacaagctt ctgctgcttc
      301 agtcgctgaa tctagctctt gtttaaattg attggaagga aagcctaaga taaccagacc
      361 ttgtgtctga taagcttggt aaagttcttc taattgttta aattgaggtg ctagaccgca
      421 ttttgttgca gtgttaacaa tgattatcgg tttgcctgca tattttttca atgaatagac
      481 agtgccgtct tctaaagtag tttcaaaatt ataaatagac ataaagtttt ctcctttcta
      541 ttttatataa taaaaagagc gctaaactga aaactagcta tttgttttcc cgtttagaac
      601 tcttttcaga caataaatat ttaataaaat acgaagataa gatagctata ttgcttctca
      661 tctttacgtt cttttagaaa ctatgcttct acttcagacc attcatcgcg ttttgctaaa
      721 aattcacgag ccaaagcttg tgcaccttct agtgtatggc tggcagccca gccacattgt
      781 gtttcattgc ttgcaggtac ttcagtagct tcaagaacat ctttcattgt tgcttcaaga
      841 acatctaaaa tgtcttcgta gctttcatga ttcaatacag tcaagtaaaa gcctgtttgg
      901 catcccattg gagaccagtc tacgatgtaa tctgcgtgat tgcggattag ttcggcagtc
      961 aagtgctcaa gtgaatgtag acttggcatg tccatgtgtt ctttatttgg ttgtttaaag
     1021 cggacgtcgt atttaatgat gacatcgcca tgcgcacccg tttttcgatc agctacacgt
     1081 acataaggtg ctttgacttt tgtatggtca agattaaaac tttcgacatt cattctcatt
     1141 tgtaagactc ctatctgttt gttcattcgt tttgatgctt ttcactaaag aattatttag
     1201 tgaaatttac aaaaacgtct ttctacttga ttctaacact tatcgtttcc aaggtgtagc
     1261 aatctgaaat attttttaga tttttctgct aagtcgttta ttgggacttc tagttcttga
     1321 ttctgtcaaa tgcctgagct aaatcgttta tgagatcctg accatactca attcctacag
     1381 aaagacggat caagccgtcc tttatgccag cctcttctcg tttttctttt ggaatagacg
     1441 catgagtcat gacagaagga atttccacta aactttctac gccacctaaa ctttctgcta
     1501 aagtaaatag ttggagtgat tcaacaaatg gaatagcaga ttcttcattt tttaaagtaa
     1561 aagaaatcat tccgctaaag ccgctcatct gctgcttagc aactccatgg agttcatgat
     1621 cgggtaatcc tggatagtag accttttcta ctgctggatg agaaaataga aaatcagcta
     1681 ccacaaaagc atttttttga tgttcctcca tacgaacact tagtgtcttg attccgcgct
     1741 gaagtaacca gctatcttgg ggaccaagta cggctccgat agcattttgg taaaatccga
     1801 tctgatcggc tagatcttta tggttagaag tcactaaacc agctactaca tcgctgtgtc
     1861 cgcctagata tttggtaccg ctatggacaa cgatatcaga accgagatct aaaggtcttt
     1921 gaaaataagg agtagcaaat gtattgtcag caataactat caatccatgt tgatgagcca
     1981 gagtggcact tttttctaaa tcagtaatct ttagaagcgg attgctaggt gtttcaagat
     2041 aaagtgcttt tgtattgggt ttgatggatt gttctatttt atcaagatta ctcgtatcta
     2101 tgatcgtgta ctcgagacca tttttagtaa gaactttgtc aaatagacgg aatgttcctc
     2161 catagacatc atctcctaat aagatatgat cgccagcttg aaaaagagaa aaaacggcat
     2221 gaatcccaga aagaccagaa gaaaaagcga atcctcgaac accgccttcc aaatcagcaa
     2281 tcagttcttc caatgcaaaa cgagtaggat taccagatct tgaatattca tattgtttag
     2341 gctggccgac gccatcttgc cgataagtgg atgtttgata aataggtaca ctgacagctc
     2401 ctgttgtggg atcttcactg atgcctccgt ggatcaattt tgtttgaatg tgcatgtttc
     2461 tttcctccta taagtaaatg ttttttgata aatagcggtc tgctgcgtct ggaaaaatcg
     2521 taacgacacg atgaccaggt ggtaaacgac gaatttctct taatgctgct gcaaaagccg
     2581 caccactcga acttccaact aatagtcctt gttctcgtgc taactgccta gtatagttaa
     2641 acccttctac atcagagatt gtctcgatct gattgattga aaggggagaa agaaacggcg
     2701 gaatgaattc tacaccgata ccctcaattt cgtgaggggc tggatcacca ccatttaaca
     2761 cagagccctc tggttctaca ccgattattc gaatgtcggg atatttttct ttcaaaaagg
     2821 tagaagttcc agcaaaggtt ccaccactcc caattcctgc tacaaaacta tggatatttt
     2881 ctttgatttc atgaaagatt tctggcccta acgtatgata gtaagcagca ggattatctt
     2941 tattttcaaa ttgaagaggc agatagctgt ttgatatctc ctctgcaagt tttttacttt
     3001 tgctgatggc accgatgatt ccttgctcac taggagaatg gatgatcttt gctcccaatg
     3061 ctttcatcaa ttgttgtttt tctaagctaa acttctctgg aacaacaaaa accgttctta
     3121 atttgtatgt gagtgcggcc agagcgatcc caattcctgt atttccggca gttggttcga
     3181 tgatcgtcgt tttttcattg atttttcctt gggcaaatcc ttctttgatc aaataactgc
     3241 ccaatctatc tttgatactt cctccaggat ttaaatattc tagttttgca taaatagcag
     3301 aacctttagg catagaatag tgctgattgt caaagcgata aagcggtgtg gaaccaatag
     3361 cttctgtaat tgtcgagata atcatgtggg cctcctaaaa aaagataata aaaaaaggag
     3421 ctgactatta aatcagctcc ttgaaagaca tagagaaaaa ttgagtagat aaaaagataa
     3481 gttacctggt actcattcta tgaagggcaa ttaatagaca tgacaaatag tatgctgctt
     3541 ttatgcacca tacaacagca acgattcaat tgtttttggt tgctgactgt catgtgtatc
     3601 cctccaagta aaatttttat taacgatagc atgaaaaaaa ataaaagtca aaggcttgaa
     3661 agaaaaaaat atttcgatag catcgtttac ccgtgtttat ttttctgaca aataggcatg
     3721 agaccttaaa agatagagaa tactctagtt ttctggccgg ttgtctgata caataaagtt
     3781 gattatgata aagaaaagaa ggagcaaaaa taatgatctc agcaagtgat ttaagagccg
     3841 gtatgacttt cgtacaagac ggcaaactga ttaaagttct ggatgcaagc caccataaac
     3901 ctggtaaagg aaatacagtt atgcgtatga aactgaaaga tgtccgttca ggtgcaacat
     3961 acgacacaac gttcagacca gaagaaaaat ttgaaaaagc acatatcgac acaaaacctg
     4021 tacaatattt gtacacgatg gatgatacag ctttcttcat ggatttggaa acatatgagc
     4081 aatatgaaat tcctgtagaa acagtagaag aagaaatgaa atatatccta gaaaacatgg
     4141 aagtaaaaat ccaatttttc ggtagcgaag tcatcggcgt tcaattacca acaactgtcg
     4201 tattacgtgt tgtggaaaca caaccttcca tcaaaggtgc aacagtcact ggttcaggta
     4261 agccagcgac aatggaaact ggcttagtag tcaatgttcc tgacttcatc gaaacagatg
     4321 aattgttgga aatcaataca gcagaaggtt cttatgtaaa acgtgcgtca aaataattcg
     4381 ctaacggatt gataagaatt tgaaaacaga gcttgacctc ttgaaaggca gatcatcgga
     4441 tctgcctttc aagaggtttt ttgtggaatc agcaaattga aaaataaaaa agtatttgat
     4501 tttatttata ttttgaaaga tgaatggcaa aagctagaga aagaaaaaac tgcacttatt
     4561 tatgaagaaa ttgaaatgat tcataaaatt gaaaatatcc gaactattcc gaactatatt
     4621 gaagcgaaaa tattttttca tcactaaata gtgttgaatt tatgttttta tgtataaata
     4681 gcttctaata gttcgggtat tttgttttta taaccatttt tattcttat
//
LOCUS       DAZKKW010000139         4660 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.141, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000139 DAZKKW010000000
VERSION     DAZKKW010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4660
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.141"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(59..343)
                     /locus_tag="ACG8PO_002674"
     CDS             complement(59..343)
                     /locus_tag="ACG8PO_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0207932.1"
                     /translation="MGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAYSPTLILSTLTA
                     GAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQYFG"
     gene            complement(514..2742)
                     /gene="topB"
                     /locus_tag="ACG8PO_002675"
     CDS             complement(514..2742)
                     /gene="topB"
                     /locus_tag="ACG8PO_002675"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFQ0207933.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            complement(2916..3326)
                     /locus_tag="ACG8PO_002676"
     CDS             complement(2916..3326)
                     /locus_tag="ACG8PO_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFQ0207934.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            complement(3443..4606)
                     /locus_tag="ACG8PO_002677"
     CDS             complement(3443..4606)
                     /locus_tag="ACG8PO_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFQ0207935.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGGQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
BASE COUNT         1207 a         1005 c          782 g         1666 t
ORIGIN      
        1 gaaaaaatga caaagaaaaa agtttgtgac atgacttttg tcacaaactc tcgtattctt
       61 aaccaaaata ttgatgaaat acttgtgtaa taactggtag aaaataaata atgatcatag
      121 ccgctaccac caccaacaat tgttttcgct gtttctttcg ctttagcatg cttgtcgttt
      181 tatgaaaaaa ataagtagcg cttttagctc ctgcagtcaa agtcgataaa atcaacgttg
      241 gtgaatatgc tgtttgattg tgcgtcaatg acaacaactt cgtcccgtaa tcaggatact
      301 ttagaaatgc ttggtacaca gccacaatcg tgattgctcc cataagataa tttaaaaatg
      361 aatgcacttt tttcaaataa aaaaactcct tgctatctat ataaaatcag cctaccccaa
      421 cgtttttgct tgattcactg atatttttta gttttattgg tatttatatt ctcacttttc
      481 ggaataaata ttccgctatt tcattgtttt catttactct acttcgtttt cattattttt
      541 tcttgatgtt tctttgtttc catcaactgc ttttgttcca ctgttttcga tccatggaaa
      601 agaaaaaccg attgtcatat cttcttttaa tactaaaact gcatcgaatt tccctttttt
      661 accagtaaaa cctttgacaa ctttacttgt ctttcctttt gaaagcaaat ctctgagatt
      721 cgttttagtc agcttctttt tagctactgt tggccacaat ttgaagtcgc attccggatt
      781 caagcatgta gcgattttag gataaagcat gactattccc tttttacatt ttggacatct
      841 tcctaattga tcgtttttct tctccatcgc ttttatctga cgtgtcccgc taatctcatc
      901 tgaaaagtct accttgtcga tttgattagg aacttctgcc aataaatgta ggatgaattt
      961 tttgatattc tctaaaaaaa cagatggtgt cccctcttgt ttaccaattt ttctcaagta
     1021 cccttcccag cgcgcagtca tttctgcgct cgtcagtaat ggttcattgg ctactgcctt
     1081 acaaagagtg attccttttg gcgtaacagt tatttcgttt ttattgactt cgatgtaatg
     1141 cttctgctta agattttcaa tgatattggc tcgtgtagct tctgtcccta ttccttctac
     1201 ttctttgaga atttcttgcg cttcatcatt atccagcgtt tttcccgcag ttttcattgc
     1261 tgtgatcaac gttccttcgg tatagggttt tggaggttgt gtttctttct ccatactttt
     1321 cagttctact tctgcttctt cattttctac gataggagga agagtctgag cttctttctt
     1381 ttttgacttg atgcgtaaga tttcctgcca accttgctgt aatgggatct ttccgatgct
     1441 ttttagcttt aattgctgta cgcccgtcat tacggtcgtc tcttcgtaga tataatctgc
     1501 agcaaacata gccacagtag tgcgcaaaac ttgcaaatag attgcttgct gtaatttgga
     1561 aagcttcgca aagcgtgcag gagtcggaat cgtctttgtc ggaataattg catgatgttc
     1621 ttgaactttt ttcgcatcga catatcgttt ctttgctttg atctcagttg tctgtacttc
     1681 ttctattcct aaaaattttt tataattttg cagatgttta accagatact gatgctcatt
     1741 ttctgtaatg taaaaggaat ccgttcttgg atagcttaga aacttgcttt cgtacaatcc
     1801 ttgagcagca tcaagtgtag ctttggcact tgctttcatt agctgattca tctttgtctg
     1861 caaactagat agcgaaaata aacgtggact atgcaacctc ttttcctttt tcgtaacttc
     1921 aagaatccgt ccgttttgct tacctaaatg cgctccaagt ttttctattt ccttcgttaa
     1981 ctcttcttgg gttttaaatg ctttttttgg tacgattttt ccagaaaact gccctttgtt
     2041 tgtcgtaatt aaaccttctc cttcaaagta cgtttctttt ttgaagtgtt tgatttcttc
     2101 ttgcagctga taaatcatat agagtgtcgg tgtttgtaca cgaccaagag aaaaactgcc
     2161 atcaatccct tttcgttgaa gtagcaagct atataatgga cttccattca ttccaatcag
     2221 ccaatcagcg ttctgccttg cttgcgcttc tttatatttt ggaaaatagt tctttccagg
     2281 ttggagattc ttaaaacctt catagattgc ttctttttca agagaattga tccataaacg
     2341 taaatagttc ttttccttag aaaatgcgtt tgcttgtcga ataatcgacc acgcgatggc
     2401 ttcaccttct cgatcaccat ctgttgcaat aatgatggta tcggctgact gcaattctcg
     2461 ttttacgatc gaaaattgtg cacgtttgtc cattggaact tcgtaacgga aggtttctgg
     2521 gaaaataggc aaattttcta aagaccattt tgcccaacgc tcttcataag ctcctggagc
     2581 aaccatatct accaaatgtc caaaaccata agtgatggtt acttcaccag caaacaatgg
     2641 atcgctgatt tcataatacc cttcatgccg cttttgctgt tggaaggttt cggcataggc
     2701 ttttgcttgt gaaggttttt cagctagtat caccgtcttc atcttctgac ctccgttact
     2761 taattttttg cggacttctc tattatggaa catattttcg catctggcaa taaaaaaaag
     2821 ataccgtata gtcgaaatag acacaaaaac atcaagcaaa caaacgatga tccgtttgct
     2881 gcttgatgtt tccagaaaat attcatcttt agctgtcact cttggttaat ctgccagttg
     2941 atgccaaatc gatcagttac catcccatac aactgagccc acggaacagc atccagtggc
     3001 atctcaatct taccaccctc agctaagctt tcaaataggc gcttcgtttc ttcttcattt
     3061 cttgtctgga tgactaaaga aacattattt cccacaacta attcaggacc aattccttct
     3121 ggcgtatctg caaacataac taatgtatca taaatgttca gccgtgcatt cgtgattaga
     3181 ttttttgctt cttctggcaa gttcatttgt tctattccag gcatttcacc gtatgtggtg
     3241 atacctgatg cttcagtctg aaaaactttt tcataaaaag cgatggcttc acgcgactca
     3301 tttttgaagt tcagatagat atccaatgta aacagctcct ctcttggtta aatctaccat
     3361 aagaaagcgt tttttacgag tgaatcgttt gattgattca tgacaaaagc cttgcgcagc
     3421 ttcttcttga tcttttttat gatcatttat tcaaaaaatc tgtaatagca gattgattca
     3481 tttgattatc aatttcaaga atactgccag cataatcact ttgcccataa ttccacgaac
     3541 cgtctactgg cacacttatt cgatcaatgc caccagctcc tcgtgcaatc gaaaagaggt
     3601 ttttcgtcaa gaaccaagca gtgacatctg tggatgtata tcctaaagct ttaccagctg
     3661 cataaggtgc acggatcaga ttcaatggat tcttcaattg gctgaaaata gcgttcatta
     3721 cttgctgttg gcgtctgaca cggccaaagt ctccttgttc atccattcgg aaacgtgcgt
     3781 actgcaaaag aacatgtcca tccatcttct ggcccccttt tttaatatct acaccatcca
     3841 gattcagatc tttttctgca tcgatcttca ctccgttcat gaataaggca tcgatcactt
     3901 tttcaaacga tttgaaatcg actttcgcat agtattgaca atcgattcca aaattttcta
     3961 ctaaagtttg acgcactaga tcggctcctc cgtatgcata agctgaattg atcttatttt
     4021 gtcctacccc tgggatcgta acgaagctgt cacgcataaa agaaatcagt ttcggcttat
     4081 gggaaggacc atccaactgt aagaccatga tcgtatcggc tcgcccggca tcttctccgc
     4141 gtgtatcact tccaaggatc aaaatattat gcgcaccatt cgtcgctttt tgcccatgaa
     4201 aggtttctga agtcgctgcc tctaagcttg tgtcatgttc agctacatat ttaccaataa
     4261 aaaatgagat acctgaataa aggatcaaaa caagcaaaat cgatagaatg atttttttcc
     4321 acgatcgctt tcttcgaggt tttaacggtt tgttcttctt ttgccgattg aaaaattttt
     4381 tatttttttg ttgttcttct gctcttggtg catctctgta tatctgttgc tcgtattggt
     4441 tcaaatgttc aaatgaatgc tgctctcttt cataaatacg ggtagcatct tcctcatttt
     4501 ccatacgcgt tcgttttcgt tctcttctag caaaaatact ccccgtatcc cgcttcagag
     4561 gtttcgcctt cttatggatg tccttatagc gatccatccg actcattgtg gttcctcctc
     4621 gtttcttact gataaataaa ggatacgata accaatcgac
//
LOCUS       DAZKKW010000140         4532 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.142, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000140 DAZKKW010000000
VERSION     DAZKKW010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4532
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.142"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(11..985)
                     /gene="lacD"
                     /locus_tag="ACG8PO_002678"
     CDS             complement(11..985)
                     /gene="lacD"
                     /locus_tag="ACG8PO_002678"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009025 - tagatose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HFQ0207936.1"
                     /translation="MKKMSIEKQERLKRLTDEAGIISALAIDQRGALKKMINQYKEAT
                     SEDIIQFKQMVSQYLTPYASAILLDPEYGLPAAALREKECGLLLAYEKTGYDAKMPGR
                     LPDLLNSWSVKRLKEVGADACKFLLYYDVDEPVEINVQKQIVIERIGSECEAEGIPFF
                     LELLSYDATIGDPSSLDYAKVKPHKVIEAMREFSKERYQVDVLKVEVPVNMRFVEGYG
                     QDTCYSQAEASAYFYEQSKVTEQPFIFLSAGVSAALFQDTLRFAKQSGSTFNGVLCGR
                     ATWAKGVAPFVTEGEEKASSWMLEEGKQNIKELSTLLEETATSIYVNK"
     gene            complement(982..1440)
                     /locus_tag="ACG8PO_002679"
     CDS             complement(982..1440)
                     /locus_tag="ACG8PO_002679"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose PTS transporter subunit IIA"
                     /protein_id="HFQ0207937.1"
                     /translation="MTIIHEENIFLQQPLDNQEEAFKFLAKQTFCLGIAHDEKEVYQK
                     LLEREKEGTTGIMSGFAIPHAKSQTINEPSLIIVTLKKGIEWNSLDRQSVDFIFALFI
                     PDSEAGTTHLKLLSLVARSLMRKEVTEQLRAAKSKIEIATILTKEIGEDI"
     gene            complement(1437..2900)
                     /locus_tag="ACG8PO_002680"
     CDS             complement(1437..2900)
                     /locus_tag="ACG8PO_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFQ0207938.1"
                     /translation="MGKYQLIAATGCPTGIAHTYMAQEALEQAARKKGITIKVETHGQ
                     IGIENELTAEEIKQADAVIIAADKDVHSERFVGKRVIDVSVSTGIKDAQRLIDEALAG
                     KGTVLADNEAADTLEPEAISGANFGRSIYKNLMNGVSHMLPFVVAGGVLIAISFAVWG
                     IYSSDPDHAQYNATAAMVKAVDDAAMGMMVPVLAAYIAEGIAKRPGLVVGFVGGIVAM
                     NGGTGFLGGILAGFLAGYFILFLQKLLIILPKQLDGLKAIVLYPVIGVAFIGIVMSLL
                     AGPMKAINEGMMSFLAGFEYSNPLVLGVIVGCMCAFDMGGPVNKAAYVTGTALLTQGN
                     TTFMAGASAACIAPPLITGFATLLFGKYFDTNDRNAGLVNFILGSTHITEGAIPFAAK
                     DPIRVLPTMMLGSSIAAVLTYLFGVQVPAPHGGFLVLPVVTGKIQWVTAILIGSIVGG
                     VILGLIQKKRVTDKDKKRDNKATNRTVIEEGRAGGLS"
     gene            complement(2882..3814)
                     /gene="pfkB"
                     /locus_tag="ACG8PO_002681"
     CDS             complement(2882..3814)
                     /gene="pfkB"
                     /locus_tag="ACG8PO_002681"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFQ0207939.1"
                     /translation="MSIYTCTMNLAIDLFIETDEMHPFMVNRTKEDDIQANGKGVNVS
                     LVLKMLDFPSTALGFSAGFTGKYIEDYLQQKQIQTEFIEVPGMTRINVFTQVNQTGEE
                     YKLVNQGPEIPQTAVHNFLNQIRALQAGDYLCVSGSLPRGLSPKVLIEISRICLIIDS
                     SDQEVMDCLPYRPFLLKPNEEELSSWFGRKMVTDEDYFVYGQRLVESGAENVLLSLGE
                     KGAILFTKDRVIRGNSPKGKVVNTACAGDAMLGTFLAGYMNRRSLDETLRKSIAAGSS
                     TAFRKGLTDFLDVEELSKQIKIKEERFERWENIN"
     gene            3956..>4532
                     /locus_tag="ACG8PO_002682"
     CDS             3956..>4532
                     /locus_tag="ACG8PO_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFQ0207940.1"
                     /translation="MNHKLSETEQYLWNFIEHLILEIPNYSIVKLSEQANVSTATIVR
                     TMKKKGYEGFTSFKHHLKEGENKNINFSFLDKVDKGIRRAILKNEQEVVRTINMLEIG
                     NIEDAIQRIKFADRVFVFARGFSEMIGQEMLVKLQLTGKNCQMHTDPEIIKSISRKLT
                     KKDSVIFVSLNGEKTNKNIKNKIYVFIFQFFK"
BASE COUNT         1355 a         1020 c          724 g         1433 t
ORIGIN      
        1 atcctttctc ttacttgtta acataaatag aagttgctgt ttcttctaat agtgtactta
       61 attctttaat attttgtttt ccttcttcta acatccaact actcgctttt tcttcgcctt
      121 cggtaacaaa aggtgctact cccttcgccc atgttgctcg cccacataag acaccattga
      181 atgtcgaacc tgattgtttt gcaaaacgca atgtatcttg aaatagtgca gcgctgactc
      241 ccgcacttaa aaaaataaac ggttgttcag tcactttcga ttgttcatag aaatacgcgg
      301 atgcctctgc ttgactataa caagtatctt gcccatatcc ttccacaaat ctcatattca
      361 ctggtacttc tactttcaat acatccactt gataacgttc ttttgaaaat tctcgcatgg
      421 cttcaatcac tttatgcggt tttacttttg catagtcaag agaagacgga tcgccaatag
      481 ttgcatcgta agaaaggagc tctaaaaaga aaggtatgcc ttctgcttcg cattctgaac
      541 caatccgttc tataactatt tgtttttgca cattaatctc tactggttca tcaacgtcat
      601 agtacaataa aaatttacaa gcatccgcac ccacttcttt taaccttttc actgaccaac
      661 tattcagtaa atctggtagt cggcctggca ttttcgcatc atagccggtt ttttcataag
      721 ctagcaacaa accacattct ttctctcgaa gcgctgctgc aggcagacca tattctggat
      781 cgagtaaaat agcggaagca tacggagtca ggtactgaga aaccatctgt ttaaattgga
      841 tgatatcttc tgatgtagct tctttatatt ggttgatcat tttcttcaaa gcaccacgtt
      901 ggtcgatagc tagcgcgcta ataattccag cttcatccgt caatcgcttc aaacgttcct
      961 gtttttctat actcattttc ttcatatatc ttcacctatt tctttagtta atattgtagc
     1021 tatttctatt tttgattttg cagctctcaa ctgttctgta acctcttttc gcatcaatga
     1081 tcgtgcaacg agagacaata atttcaaatg agtcgttcct gcttctgaat ctgggataaa
     1141 taacgcaaaa ataaaatcaa ctgactgtct atctaaactg ttccattcaa ttcctttttt
     1201 cagtgtgacg ataatcaaac taggttcatt gattgtttga gacttcgcat gagggatagc
     1261 aaaacctgac attattcctg tagtaccttc tttttctctt tccagaagtt tttgatatac
     1321 ttctttttcg tcatgagcga ttcctaaaca gaatgtttgc tttgctaaaa acttaaatgc
     1381 ttcttcttga ttatctaatg gctgttgtaa aaaaatgttc tcttcatgaa tgattgtcat
     1441 gataaacctc ctgctcttcc ttcttcgata actgtacgat ttgttgcttt attatccctc
     1501 ttcttgtctt tatcagttac tcttttcttt tggatcaaac ctagtatcac tccaccaacg
     1561 atcgaaccaa taagaatcgc tgtaacccat tggatttttc ccgtcactac aggtaacact
     1621 aaaaaaccgc catgtggggc tggtacttgg acaccaaaca gataggtcaa gacagcagca
     1681 attgatgagc ctaacatcat cgttggtaat actcggatcg ggtctttcgc agcaaacgga
     1741 atagctcctt ccgtaatatg agttgatcct aaaataaaat taaccaaacc tgcattacga
     1801 tcattagtat caaaatattt accaaacaat aaagtagcaa aaccagtaat caacggtggt
     1861 gcaatgcaag ctgctgacgc accagccata aaagtcgtgt ttccttgtgt caaaagagct
     1921 gttcctgtta catatgccgc cttattaacc ggtcctccca tatcaaacgc acacatacag
     1981 ccaacaatca cgccaagaac aagtggattc gaatattcaa atccagctaa aaagctcatc
     2041 attccttcat ttatagcctt cattggacct gccagcaaag acatcacgat gccaataaat
     2101 gctacaccaa taactggata taagacgatt gccttcaagc catctagttg tttaggtaaa
     2161 attatcagta gcttttgcaa aaataagata aaataacctg ctaagaaccc agctaaaata
     2221 cctcctaaaa atccagtacc gccattcatg gctacgattc caccaacaaa tccaacgact
     2281 aaccctggac gtttcgcgat cccttctgca atataagctg ctaaaactgg aaccatcatt
     2341 cccattgctg catcgtcaac tgcttttacc attgccgctg tagcattgta ttgtgcatga
     2401 tctggatcag aagaatagat tccccaaacg gcaaatgaaa tagcaatcaa tactccgcca
     2461 gctaccacga aaggcaacat atgagagaca ccgttcatca aatttttata gatgctacga
     2521 ccaaaattag caccacttat cgcttctggt tctaaagtat cagcagcttc attatctgct
     2581 aagacagtac cttttcctgc caatgcttcg tcaatcagcc gttgtgcatc cttgattcct
     2641 gtactgacag aaacatcaat gactcgtttt cccacaaatc tttctgaatg gacatctttg
     2701 tctgcggcaa taatgacagc atctgcctgt ttgatttctt cagccgttaa ttcattctcg
     2761 atcccaattt gtccatgggt ctccactttg atcgtgatgc cttttttcct agctgcttgc
     2821 tctaatgcct cttgcgccat gtaggtgtgc gcaattcctg tcggacaacc ggttgctgca
     2881 attagttgat attttcccat ctttcgaacc tctcttcctt gattttgatt tgtttactta
     2941 attcctccac atccaaaaaa tcagtcagtc ctttacgaaa agcagtagag ctgcctgctg
     3001 ctatgctttt acgtaacgtt tcatcaagcg atcgtcgatt catgtatcct gctaaaaaag
     3061 tgcctaacat ggcatcacct gcacatgctg tattgacaac ttttcctttt ggcgagtttc
     3121 ctcgaatcac cctgtctttc gtaaaaagaa tcgctccttt ctcaccgagg gataacaaaa
     3181 cattttctgc gcctgactct actagtcgtt gtccataaac aaagtaatct tcatctgtaa
     3241 ccatttttcg tccgaaccaa gaagaaagtt cttcctcatt tggtttcagc aaaaacggtc
     3301 gatagggtaa acaatccatg acttcttgat cactactatc aataatcaaa caaatccgac
     3361 tgatttctat taaaaccttt ggagacagcc ctcttggtaa actcccagaa acacaaagat
     3421 aatcccctgc ttgtaatgct cttatttgat ttaaaaaatt atgaacagct gtctgcggta
     3481 tttcaggtcc ttgattcact aatttgtatt cttctcccgt ttgattgact tgtgtaaata
     3541 cattgatacg cgtcatacca ggcacttcaa taaactctgt ttggatttgt ttctgctgta
     3601 aatagtcttc tatatacttt cctgtgaacc cagcagaaaa acctaatgcc gtacttggga
     3661 aatctagcat ctttaatact agtgacacat tcaccccttt tccgtttgcc tgaatgtcat
     3721 cctcttttgt gcggtttacc ataaaaggat gcatctcatc tgtttcaata aatagatcaa
     3781 ttgccaaatt catcgtgcat gtgtagatag acataaccaa cgacctcctt gattctatta
     3841 tgaaatacgc gaaatagaaa atgttttctt aatcgtccca acacaaagaa aacctttaca
     3901 tttataaact cgtcgtgctt tttttaacta acttttggtg aaagaaggaa agaatatgaa
     3961 tcacaaatta agtgaaacag agcagtacct ctggaacttt atcgaacacc tcatcctaga
     4021 gatccctaac tattccatcg tgaaactaag tgaacaagca aatgtctcaa cagccaccat
     4081 tgtgcggaca atgaaaaaga aaggctatga agggtttact tcgtttaagc atcatctaaa
     4141 agaaggagaa aacaagaaca tcaatttttc atttttagat aaagtagaca aaggaatccg
     4201 ccgagccatt ctaaaaaatg aacaagaagt ggtacgtaca atcaatatgt tggaaatcgg
     4261 gaacattgaa gatgcaatac aaagaatcaa atttgctgac cgggtttttg tctttgcccg
     4321 aggattttca gaaatgattg gccaagaaat gctggtaaaa cttcaattaa cgggaaaaaa
     4381 ttgtcagatg catactgatc cagaaattat caaaagcatc agtcggaaac taactaagaa
     4441 agacagcgtt atttttgttt ctttgaacgg tgagaaaaca aataaaaaca tcaaaaataa
     4501 gatttatgtc tttatttttc aattttttaa at
//
LOCUS       DAZKKW010000141         4262 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.143, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000141 DAZKKW010000000
VERSION     DAZKKW010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4262
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.143"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1..371)
                     /locus_tag="ACG8PO_002683"
                     /pseudo
     CDS             complement(1..371)
                     /locus_tag="ACG8PO_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(573..968)
                     /gene="panD"
                     /locus_tag="ACG8PO_002684"
     CDS             complement(573..968)
                     /gene="panD"
                     /locus_tag="ACG8PO_002684"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HFQ0207941.1"
                     /translation="MKVTMLKGKIHRATVEQAELDYVGSITVDMNLLEAAGIYEYEKV
                     QIVDVNNGNRFETYTIAGERGTGMICLNGAAARCVSTGDKIILMAYCELNENEVKDHR
                     PKVVFVDDNNKVERVTSYEKHGRLSDIIL"
     gene            complement(978..1826)
                     /gene="panC"
                     /locus_tag="ACG8PO_002685"
     CDS             complement(978..1826)
                     /gene="panC"
                     /locus_tag="ACG8PO_002685"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HFQ0207942.1"
                     /translation="MVIFKTVNDVRSQVKEWKKQGLKVGLVPTMGYLHEGHESLIRKA
                     SKENDKVVVSIFVNPTQFGKNEDLGSYPRDLERDIEVCTRGRATAIFNPEVEEMYCDN
                     ASTFVNITGLTEGLCGASRPIHFRGVCTVVSKLFNIIPADRAYFGEKDAQQLAVIKRM
                     VRDLNIDIDVVGCPIIREEDGLAKSSRNTYLSLEERSSATILNKSLTLAKEALNNGER
                     DSLKIIEIISKNINTYNLAKIDYVEVVDSLSLQRVNYIEKSVLVAIAVFIGKTRLIDN
                     FTFELQ"
     gene            complement(1841..2668)
                     /gene="panB"
                     /locus_tag="ACG8PO_002686"
     CDS             complement(1841..2668)
                     /gene="panB"
                     /locus_tag="ACG8PO_002686"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HFQ0207943.1"
                     /translation="MKNTAVTFKESKLRNEKLTMLTAYDYSTAKIIDEAGINGILVGD
                     SLGMVCLGHEDTLSVTMEDMIHHTRAVTRGAKNTLVVADMPFMSYQTSVYDSVVNAGR
                     LIKEGRAQVVKLEGGIEVCDKIEAIVKASIPVMAHIGLTPQSVNAFGGFKVQGKDKEA
                     AKELIRAAKAVEKAGAFAVVLECVPTKLAELISKEISIPTIGIGAGAGCDGQILVYQD
                     MLGMYSDFTPKFVKKYANLSEEMNKAFTKYIEEVKDGVFPGPEHGFKISDDVLEKLY"
     gene            complement(2680..3540)
                     /locus_tag="ACG8PO_002687"
     CDS             complement(2680..3540)
                     /locus_tag="ACG8PO_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002384409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rossmann-like and DUF2520 domain-containing
                     protein"
                     /protein_id="HFQ0207944.1"
                     /translation="MEVIIMNVGFIGAGKVGCSFGKYFQEHKIQVTGFYSKSEDSSLA
                     ASNFTSSKQYLNLRELVDENDTIFITTPDGQIQEVWQEIKNYQIKNKLICHCSGAISS
                     DIFSNIQDYGAYGYSVHPMFSISDKYNSYKKLKKSFITIEGDEKYAKFLCDFFKKLGN
                     STVIVSKENKSLYHAASVVSSNLVLGLINTSVTYLIQCGFTEKMAIEALYPLIEFNIR
                     NIKEKGIIESLTGPVERCDISTIKGHCEVLSDEDRALYMLLSKNVLEIAMLKNINRDY
                     SELEKYLGES"
     gene            3778..>4262
                     /locus_tag="ACG8PO_002688"
     CDS             3778..>4262
                     /locus_tag="ACG8PO_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFQ0207945.1"
                     /translation="MTKFTIEIMQTLLNKGDLDELFQDHLERAINSLLQAELTAFLDY
                     EKYDRNGFNSGNSRNGNYSRTFKTEYGELNLTIPRDRNGEFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMAEISKLIEKMYGHHYTPQTISNMSKLVAEDVLAFKERTLEANYSVI
                     F"
BASE COUNT         1332 a          816 c          508 g         1606 t
ORIGIN      
        1 ttgataatag taggtctgac gagaaatatt tagaattttg cacatcgctg atatggaata
       61 tttatgcttg ttggcatcaa ttacttgtct tttcgtccga atatcagcgc cgcttgcttt
      121 aaaatatcat tctccatttc gagttgctta tttttctttc ttagtgcgat caattctgct
      181 tgttctggtg ttaagttgtc tctttttttg aatgagcccg ttgattgtgc ttgcttcatc
      241 catttatcga aagaagaagg cgtaagctca tactctcgaa taatttctgc gcgaggctta
      301 ccagcgagat agagattgac gatttgttgt ttgaattctt ttgaataggt tcttcgttga
      361 cgtcttgaca tgaaaaatcc tccagtgtgt tttttattat tctacacacc ttattttttc
      421 tgtctagtct agtgtagccg attcacataa aatggacttt gtctacagtc tgtatcttct
      481 ctttttgtgt ctactttatt gacatctatc caattagtaa tcaataaata tttcaataat
      541 agtctagcta tatataaata acattacaaa acctaaagaa ttatatctga taaccttcca
      601 tgtttttcat atgatgttac tctttcaact ttattattat catcgacaaa aacaacctta
      661 ggtctatgat ccttaacctc attttcattt aattcacaat atgccattaa aattatttta
      721 tctccagttg atacacatct agctgcagct ccattcaagc aaatcatccc tgttcctctc
      781 tctccagcta ttgtataagt ttcaaatcta tttccattat taacatcaac tatttgtact
      841 ttttcatatt catatattcc tgctgcttct aataaattca tatcaacagt aatacttcct
      901 acataatcta actcagcttg ttcaactgta gctctatgta tttttccttt taacattgta
      961 actttcatta tagcccccta ttgtaattca aatgtaaaat tatctattaa tcttgttttt
     1021 cctataaata ctgctattgc aactaaaact gatttttcaa tataatttac tctttgtaat
     1081 gataaagaat caacaacttc tacataatca atctttgcca aattataagt atttatattt
     1141 ttacttatta tctcaattat ttttaagctg tctctctctc cattatttaa agcttcctta
     1201 gctaaagtta aagatttatt taaaatagta gctgaagatc tttcctctaa acttaagtat
     1261 gtgtttctag aacttttagc tagtccatcc tcttccctta taattggaca ccctacaacg
     1321 tctatatcaa tattaaggtc cctaaccatt cttttaatta cagctaactg ttgtgcatcc
     1381 ttttctccaa aataagctct atctgctggt attatgttaa ataatttact tacaacagtg
     1441 caaacccctc taaaatgtat tggcctacta gctccacata aaccttctgt taatccagtt
     1501 atattaacaa aagtacttgc attgtcacaa tacatctctt caacttcagg attaaatatt
     1561 gctgttgctc tacctcttgt gcatacttct atatctcttt ctaagtctct tggataacta
     1621 cctaaatctt catttttccc aaactgagtt ggatttacaa agatacttac aacaacttta
     1681 tcattttctt ttgatgcttt tctaattaaa ctttcatgac cttcatgtaa atatcccata
     1741 gtaggaacta atcctacctt taagccttgt tttttccact cttttacttg acttctaaca
     1801 tcattcactg ttttaaatat aaccatattt cttgaccctt ttaatataat ttttctaaaa
     1861 catcatcact tattttaaac ccatgttctg gacctggaaa aactccatct tttacttcct
     1921 ctatatattt tgtaaatgct ttattcatct cttctgataa attagcatat ttcttcacaa
     1981 atttaggggt aaaatcacta tacatcccca acatatcttg atatacaaga atttgtccat
     2041 cacaccctgc acctgctcca ataccaattg taggtattga tatctcttta gaaattaact
     2101 ctgcaagctt agttggaaca cattctaaca ctacagcgaa tgctcctgct ttttctacag
     2161 ctttagcagc tcttattaat tctttagcag cttccttgtc ctttccttgt accttaaatc
     2221 ctccaaatgc attaacagat tgaggtgtta aacctatatg cgccataaca ggtatagaag
     2281 cctttactat agcctctatt ttatcgcata cttctatccc tccttctaat ttaacaacct
     2341 gagctcgtcc ttcttttatt aatcttcctg cattaactac tgaatcatat actgaagttt
     2401 gataagacat aaatggcata tctgcaacaa ctaaagtgtt cttagctcct cttgtaactg
     2461 ctctagtatg atgaatcatg tcttccattg ttactgaaag tgtatcttca tgtcctagac
     2521 ataccatacc aagagaatct cctactaata ttccatttat cccagcttca tcaattattt
     2581 ttgctgttga atagtcataa gctgtaagca ttgttaattt ttcatttctt aatttagatt
     2641 ctttaaatgt aactgctgta tttttcatct atttctctcc taactctctc ctaaatactt
     2701 ttctaattca ctataatctc tattaatatt ttttaacata gctatctcta atacattttt
     2761 tgataatagc atatataaag ctctgtcttc atctgataaa acttcacagt gacctttaat
     2821 tgttgaaata tcacaacgtt caactggccc tgtcaaactt tctataattc ctttttcttt
     2881 tatatttcta atattaaatt caattaatgg atataaagct tcaattgcca ttttttcagt
     2941 aaatccacat tgaattagat atgtaacgct tgtattaata agtcctagca caaggttact
     3001 agatactact gatgcggcat gataaagaga tttattctcc ttagatacaa ttacagtact
     3061 attacctaac tttttgaaga aatcacataa aaactttgca tatttttcat cgccttcaat
     3121 tgttataaaa gattttttta attttttgta tgaattatat ttatctgata ttgaaaacat
     3181 aggatggaca gaatagccat aagcaccata gtcctgtata tttgaaaaga tatctgaaga
     3241 tattgcacca ctgcaatggc agattaattt gtttttgatt tgataatttt taatttcttg
     3301 ccatacctcc tgaatttgac catcaggagt cgtaatgaaa atagtatcat tttcatctac
     3361 taattctctt aagtttaaat attgtttact agatgtaaaa ttcgaagctg ctaatgacga
     3421 gtcttcgctt ttactataaa atcctgttac ttgtatttta tgttcctgga aatacttccc
     3481 gaatgaacaa cctaccttgc cggctccaat aaaaccaacg ttcataatta tcacctccat
     3541 aaagaagtga gactacaagt cccattctta cgatatgagc ttaaaaataa aaattaaatc
     3601 aaatatacag tatgtttttt tcaaatatca tccatagtta atttatcata atatcccata
     3661 ttttaaaata aaagatagcg taaaatattt tgtgtaaata gaaatttagg gtagaaaaaa
     3721 aagtccattc tgtaaaatta aagttaccac acagaaattt tataggagga ctttctcatg
     3781 actaaattta ctatagaaat tatgcaaaca ctactcaata aaggggattt agatgaatta
     3841 tttcaggatc atttagagcg agcgattaat tcacttctac aagcagagct aactgctttt
     3901 ttagattacg aaaaatatga tcgaaatgga ttcaattcag gaaattcccg taacgggaat
     3961 tattcacgta catttaaaac agaatatggc gagttgaatt taactattcc tagagatcgg
     4021 aatggcgaat tttctcaaca aacactacca gcatataagc gaaccaatga ttcattagaa
     4081 acgaccatta tccaattatt tcaaaaaggc attactatgg ctgaaatctc caaattaatt
     4141 gaaaaaatgt atggtcacca ctatacgcca caaacaatct ccaacatgag taaattggtg
     4201 gctgaagatg ttttagcttt taaagaaaga acgttagaag ccaattattc cgttatattt
     4261 at
//
LOCUS       DAZKKW010000142         4104 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.144, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000142 DAZKKW010000000
VERSION     DAZKKW010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4104
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.144"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(71..412)
                     /locus_tag="ACG8PO_002689"
     CDS             complement(71..412)
                     /locus_tag="ACG8PO_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFQ0207946.1"
                     /translation="MKPNEIVTVYVAFTDKNGGKRRPILVVSDKEDRVEFFGITSQYE
                     KKSDQIKRVYFPIAEWEKAGLKKQSWIDVGSLKAIPKSRENISFKKVGMLSASDTKKL
                     NRFIEKLQRKV"
     gene            complement(402..683)
                     /locus_tag="ACG8PO_002690"
     CDS             complement(402..683)
                     /locus_tag="ACG8PO_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HFQ0207947.1"
                     /translation="MKVKNPTEKSRIQVGIDKNLKENAEMILEELGLNPTTAITILYK
                     QVVARGEFPVEIKLSEEEKQGIRLQQLTKDMPVDVLDTDEKLEEWFDEA"
     gene            complement(1054..1293)
                     /locus_tag="ACG8PO_002691"
     CDS             complement(1054..1293)
                     /locus_tag="ACG8PO_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0207948.1"
                     /translation="MTKFKSKGMQWCSYYYVGNDEDLLRKTQRKGYPDKILKQIGYYM
                     PEERIEKEFCEVLFDSEQLLVLLTQNKLICQFENN"
     gene            complement(1313..1561)
                     /locus_tag="ACG8PO_002692"
     CDS             complement(1313..1561)
                     /locus_tag="ACG8PO_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0207949.1"
                     /translation="MEGMDFLDHEDLVDFGYTWKGMVGISRSLANAFYERNYAVYVLY
                     DDDTESLVDEEYKLDLENVLYGIEKEDLAKYIFSWLGQ"
     gene            complement(1576..2142)
                     /locus_tag="ACG8PO_002693"
     CDS             complement(1576..2142)
                     /locus_tag="ACG8PO_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0207950.1"
                     /translation="MFEKRKDQFPNLEAKSQKVIDGEMIELFQKSYGTIETTASCKYY
                     FQGKRNTFKGFADSDEELAKKIIDWLVSSEHVAREDTVRTTINGEVLTGTRCVCCNHE
                     FVEDEGCYRINEYGDFICQSCHEIEFPTERSWFEYMLQDYEMEKENGEVVFCTKEELS
                     KRSDDEIEDWCVWLVGADDCVAYYTTAE"
     gene            complement(2143..2688)
                     /locus_tag="ACG8PO_002694"
     CDS             complement(2143..2688)
                     /locus_tag="ACG8PO_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0207951.1"
                     /translation="MGERTQALITVKYGNSKTKASVSIHYQWGYGRYMLMDVLNQGIY
                     LINKAYDLRDKSREELAKGIFDRSTGVINFVTYESGVSAYDETGFGGRYEPYQETIED
                     VMKFSDNNDGYAHLEMVFDENGFSLDDKTRLRLFDFEGQQIGLNKFCESGKEFATKDF
                     IKGYRLLLENYGINLEEESVK"
     gene            complement(2714..3481)
                     /locus_tag="ACG8PO_002695"
     CDS             complement(2714..3481)
                     /locus_tag="ACG8PO_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HFQ0207952.1"
                     /translation="MRDRILEVNQEMVNKLTNISGNYEKYDGTLLDNYIFHDTQNIRI
                     GRSKPRAFILIKEKYLNEWNSTYEMVMTDNFEKVLDFRNALLGSDMGNSSLESIEVEN
                     KTENEGEGLIKNVLKIEQKYISEDRSFYRVTSSVGLANFATNLIGNNASESLLVMCLN
                     TKNEVIAYSEVFKGGINQSVAIPREIFQLALLNNSAKIAVSHNHPSGYEFPSENDKSF
                     TTNLKNAGKTIGIELLDHIIVTSKNTSYYSFQENGSL"
BASE COUNT         1174 a          862 c          533 g         1535 t
ORIGIN      
        1 ttcatataca taaatacgtt tatataaacg tatatcttaa tatacatata aacaaaaaag
       61 aaaagccttg ttagactttt ctttgtaatt tctcgataaa cctatttagc tttttcgtat
      121 cactagctga taacatacca acctttttga atgatatgtt ctctcggctt ttgggaatag
      181 cctttaaaga ccctacgtca atccatgatt gctttttaag tcctgctttt tcccattctg
      241 caataggaaa atacaccctc tttatttggt ctgatttttt ctcatattga cttgtgattc
      301 caaagaattc tactctatct tctttatcac taaccaccaa aataggtctt cttttaccac
      361 catttttatc tgtaaatgca acatagaccg ttactatctc attaggcttc atcgaaccac
      421 tcttctaact tttcatctgt atctagaaca tctactggca tgtccttagt tagctgttgc
      481 aatctgattc cttgcttctc ctcttcactt agctttatct ctactggaaa ctcacctcta
      541 gcgactactt gtttgtaaag aattgttatt gctgttgtag gatttaaacc tagctcttcc
      601 aatatcattt ctgcgttctc ttttaaattc ttgtctattc cgacttggat tctgcttttt
      661 tcagttggat ttttgacctt catttcatcc actccttttg agtataaaac actcattatt
      721 tctcaaattg tccacattta tgattatatt atactcttaa taaactacat tgcaagtaat
      781 ctaatcaaat taagatgcta tatcatacgg taattgaaca aattgtcgct aacccacaca
      841 tacggaatcg taatgaatga aaaaaagaca agcctattcg cagtaggctt gtctttgtgc
      901 ttctcttgta caaaatcttc acatttgtaa attaatttta gcacctttgt taggaaagaa
      961 caacttaaat gagatgctaa agaattaata gttctcaatc tagcatattc acttaatacc
     1021 atcttatctt cattcatggt tcaccacctc atcttaatta ttttcaaatt ggcaaatcaa
     1081 tttattttgg gttaacaaaa ctaatagctg ttcactatca aaaagaacct cacaaaattc
     1141 tttttctatc ctctcttctg gcatgtaata acctatttgt ttcaatatct tatctggata
     1201 accttttcgt tgggtttttc tcaataaatc ttcatcattt ccaacataat aataagaaca
     1261 ccattgcata cccttacttt taaattttgt catgtttcta tcttcctttc acttattgac
     1321 ccaaccatga aaaaatatat tttgctaaat cttccttttc tattccatat aaaacattct
     1381 ctaaatccaa tttatattcc tcgtcaacaa ggctttctgt atcgtcatca taaagaacat
     1441 atactgcata atttctttcg taaaaggcat tagcaagact tctgctaata cctaccattc
     1501 ctttccatgt ataaccaaaa tcaaccaaat cttcatgatc tagaaaatcc attccttcca
     1561 tgctgattca cactcctatt ctgctgttgt gtaatatgca acgcaatcat ctgcccctac
     1621 taaccacaca caccaatctt caatttcatc atcgcttctc ttactcaatt cctctttggt
     1681 acaaaaaact acttcaccat tttctttttc catctcataa tcttgtagca tgtattcaaa
     1741 ccaacttctt tcggttggaa attctatctc gtggcaactc tggcaaataa aatcaccata
     1801 ttcattgatt cggtagcaac cttcatcttc tacaaattca tgattacaac aaacacatct
     1861 agtacctgtt aggacttccc cattaatcgt tgttctaacg gtatcttctc ttgctacgtg
     1921 ttcactgctg acaagccaat caatgatttt ctttgccaat tcttcatcag aatcggcaaa
     1981 gcctttaaac gtgtttctct ttccttgaaa gtagtatttg caacttgcgg tagtttctat
     2041 ggttccataa cttttttgga ataattcaat catttcacca tcaatcactt tttgactttt
     2101 cgcttctaaa ttagggaact ggtcttttct tttttcaaac acttatttca cgctctcttc
     2161 ttctaggttt attccataat tttctagcaa taatctgtat cccttaataa aatctttcgt
     2221 agcaaactct tttccgcttt cacaaaactt gtttagtcct atctgttgac cctcaaaatc
     2281 aaacaatcgc aatctggttt tatcatctag actaaatcca ttttcatcga acaccatctc
     2341 taaatgagca tagccatcat tgttatctga aaacttcatc acatcttcaa tcgtttcttg
     2401 ataaggttca tagcgcccac caaaccctgt ttcatcataa gcactcactc cagattcata
     2461 ggtaacaaaa ttgataactc ctgtacttct atcaaagatt ccttttgcta attcttctct
     2521 tgatttatct cgtaagtcgt atgctttatt gattaaataa attccttgat tcaatacgtc
     2581 catcagcata tatcgaccat acccccactg ataatgaatc gacacgcttg ctttcgtttt
     2641 gctgttacca tatttgactg taattaacgc ttgtgttctt tcgcccatct tgttttcctc
     2701 ttttctctaa tttttataaa gaaccatttt cttgaaatga gtaatagctt gtgtttttac
     2761 ttgttacgat gatatggtct aacaattcaa taccaattgt ttttcctgcg tttttcaaat
     2821 tcgtagtaaa acttttgtca ttttcacttg gaaattcata tccgctcgga tgattatgtg
     2881 atactgcgat ttttgctgaa ttgtttagca aagctagttg aaaaatctct cttggtatcg
     2941 ctactgattg attaattcca cctttgaaaa cttcgctata agcaatgacc tcattctttg
     3001 tattcagaca catcactagc aaagattcac tagcattgtt tccaattaag ttagtagcaa
     3061 agtttgctag accaaccgag cttgtgactc tataaaaact tctatcttca ctgatatatt
     3121 tttgttcgat tttcaagaca ttcttaatca atccttcacc ttcattctcg gtcttgtttt
     3181 caacttcaat agattcaaga ctgctatttc ccatatcaga acccaacaaa gcatttctga
     3241 aatccaatac cttttcaaag ttatcagtca taaccatttc ataggtgcta ttccattcat
     3301 tcaaatattt ctctttgatg agaatgaacg ctctcggttt acttctgcca attctgatat
     3361 tttgtgtatc gtgaaaaata tagttatcaa gtaaagtacc atcgtacttt tcatagttcc
     3421 cactgatatt tgttaacttg tttaccattt cttgattaac ttctaaaatt ctatctctca
     3481 tgttgaacgc ttctttctgt ttttgatttt tggtttttca aaattctttt ttgtttggat
     3541 tgttttagag ggaggtggtt ttttcttcat cgctatttgg ttttgcattt tatgtttctt
     3601 cctttcgttc atttatttat ctttttcgcc tgtcttgcct tgtcgggagt gattagaatg
     3661 cgttcttatc tgccggatta atacaagtgt ttaagtaaat tattttcgta acgcttgcgt
     3721 ttagaaaaaa atttacgcca cttgtattaa tccaaagatg agataagctc taaacacttc
     3781 tgacaaggca agacaggcga aaaagataat gggcgtaagg aattataaat ggcaaaacca
     3841 aataaagagc gatgaatttc aatgctttca ggcgtagctt gaaagcctaa aagtcttgaa
     3901 gaatgaaaga ctttttacct tcaaagcaga cctagtaatc ttaaataggc gatggctatt
     3961 taagattacg gtgttgaccc actaaataaa cagaaatgga accgatcaag caaaatcgga
     4021 ctaggcgatg gctaggacgg tttgcggtgg ctttaggaaa cgttcatggt gagatgagca
     4081 tatctagcaa tgtcgggcta agct
//
LOCUS       DAZKKW010000143         4102 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.145, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000143 DAZKKW010000000
VERSION     DAZKKW010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4102
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.145"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..442)
                     /locus_tag="ACG8PO_002696"
     CDS             complement(<1..442)
                     /locus_tag="ACG8PO_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="HFQ0207953.1"
                     /translation="MGFSISFKKGTKNTSISHNNRELTDSQKINDWHKHIDFTKSDEN
                     IYLEQTNIREKYEELFGEAVEKYNAKQKRADRKIEDYLAKVRKDKKLEPQREFIVQIG
                     TLDDFRTTRDDGSSTGISEQQAEQNRVIANKILVEYFKEFKERNP"
     gene            complement(663..1025)
                     /locus_tag="ACG8PO_002697"
     CDS             complement(663..1025)
                     /locus_tag="ACG8PO_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0207954.1"
                     /translation="MGMTLADIKAQEEKLKKEQAKLRQQKKRLIEKENATMGKALRKR
                     FGFESFADFERWLSSISDDQQHEKSVDNEWLADEKSAPSERDYPDESDGLFGVIPNKK
                     SQMSESKFTSIFNDVNLR"
     gene            complement(1029..1337)
                     /locus_tag="ACG8PO_002698"
     CDS             complement(1029..1337)
                     /locus_tag="ACG8PO_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0207955.1"
                     /translation="MDKIDLREETVDRIELMYEWLADYLNLPDEDNYSEMLERMMDVL
                     NSVINADLAKSSVLTNEFVHRHLAQTTDESEKEFWLKLMSHVMQLNQISVINSKEKEM
                     "
     gene            complement(1346..1765)
                     /locus_tag="ACG8PO_002699"
     CDS             complement(1346..1765)
                     /locus_tag="ACG8PO_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0207956.1"
                     /translation="MTDLPEKMTIKNLADELGISKNTVRNQLVNAGYDLARYKDNGVI
                     MLDKELIGVVRSIYRSKGGQLTGDLPVNDAGNDFPLEKENEFLREQLAEKDRHISELT
                     EMIRDQQKLTLLQQQKNDQLLLENQELKNRKWWQFWK"
     gene            complement(1758..1991)
                     /locus_tag="ACG8PO_002700"
     CDS             complement(1758..1991)
                     /locus_tag="ACG8PO_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0207957.1"
                     /translation="MAEKQKHFGKKNWLVNLTGDNVVVVFLDSKKMSGKLLDVTQYEL
                     ILKNNKIDSPIIIPKHAVKYVYGVAESNGEDND"
     gene            complement(1994..2962)
                     /locus_tag="ACG8PO_002701"
     CDS             complement(1994..2962)
                     /locus_tag="ACG8PO_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFQ0207958.1"
                     /translation="MSKKGTIINEWEKLTAPSNRRLNVQTLVQTIKLNWSIDRITIVG
                     KLNQNIFYHTQHDVLILDFEQLMRVNEGNGYLKAVGNNGWQLLDQHDENIAYIEISKW
                     QEGKGRIDFNPNKISRFLADSMKNFIHDLFLEPHFSRADVACDLIDVPDEFITQYRII
                     DPVSFKLIYGRSGKLETVYWGSRASERQIRLYNKKIEQEMKRKVVPSEINTWWRLEMQ
                     LRRGKATEWYAMVKESLDSFVSPHFLPTDIKPTDKVMIDGLLANHSNWAILSRNSKYK
                     YRNLLKRESQNDELTNHLRETFEESADDLKKELDTWLQGLDVTEEE"
     gene            complement(3411..>4102)
                     /locus_tag="ACG8PO_002702"
     CDS             complement(3411..>4102)
                     /locus_tag="ACG8PO_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HFQ0207959.1"
                     /translation="HEYKEIMSEISELRQTAVKERESVSDELRYISRQREENEREKAL
                     VASERVKIEEEKKNMLNVKKEMAVIFQKDIVPLESFKRLPDGSYHLSGNDFVDLYQRA
                     TRATNAKRNADNMSEQYLQMLEQNTELNEYVATLEEERHLSGRKQLADLQVENKKLKE
                     ENRSLRQRLERLKNAFQKSITRLSIRFGLAEKDMGLSEELEVLEQEKTLFYRQNVSQR
                     KLERSEDEIEW"
BASE COUNT         1171 a          912 c          566 g         1453 t
ORIGIN      
        1 taggattccg ttctttaaat tctttgaaat attcaactaa aattttgttg gcaatcactc
       61 gattttgttc tgcttgttgt tccgatatgc cagtagaaga accatcatca cgagtagtac
      121 gaaaatcatc aagagtacca atttgcacga taaattctcg ctgtggttcc aattttttat
      181 ccttgcgaac ctttgctaaa tagtcctcga tttttctatc ggctcttttc tgctttgcat
      241 tatatttttc tacggcttca ccgaataatt cttcatattt ttcacgaata tttgtttgtt
      301 ctaaatagat attttcatcc gattttgtga aatcaatatg cttatgccag tcattgattt
      361 tttggctatc agttaactca cgattattat gagaaatgct tgtattcttt gttccctttt
      421 tgaacgaaat actgaacccc aagttaaaac cctccttcgg ctgtcgctaa taaaaagagt
      481 gtaaaccttt ctgttactct ttgtcaaaga gtaacatttt cctaattgtg acatggctgt
      541 cacaattcca aatgctctcc gagggtattc gctgtcacaa gtgacaacga aaaaaggagc
      601 cgaaagcgtg tcacgctctc aactcctttt acgctttatt ttgcttcacg caaaataatc
      661 gcttatcgta aattcacgtc attaaatatt gacgtaaatt tactctcact catctgactt
      721 ttcttattcg gtatcactcc gaataagccg tcagattcat cgggataatc tcgttcgctt
      781 ggcgctgatt tttcatcagc aagccactca ttatccactg atttttcgtg ttgctgatcg
      841 tcagaaatcg acgatagcca ccgctcaaaa tcagcaaaag attcaaaccc aaaacgcttg
      901 cgaagagcct tgcccattgt agcattttct ttctcaatca agcgtttttt ctgttgacga
      961 agtttcgctt gttctttttt taatttttct tcttgcgctt taatatctgc taaagtcatt
     1021 cccataaact acatctcctt ttctttggaa ttaataacgg aaatttggtt taactgcata
     1081 acatgactca ttaatttcaa ccaaaattct ttttctgatt catcagttgt ttgcgctaaa
     1141 tgcctatgaa caaattcatt agtcaaaact gaagattttg ctagatcagc attgataacc
     1201 gagttgagaa catccatcat tcgttcaagc atttcactat aattatcttc atccggcaaa
     1261 ttcaaataat cagctaacca ttcatacatc aattcaattc tatcgactgt ttcttctctc
     1321 aaatcaattt tatccatgtc acacctcact tccagaattg ccaccatttt cgatttttta
     1381 attcttgatt ttctagtaac aactgatcgt ttttttgttg ctgaagcaaa gtcaattttt
     1441 gttggtcacg aatcatttcc gttaattcag aaatatgcct atctttttcc gctaattgtt
     1501 cacgcaaaaa ttcattttct ttttctaacg ggaaatcatt gcctgcatca ttgaccggta
     1561 aatcaccagt caattgacca ccttttgacc gataaattga ccgcacaaca ccaatcaatt
     1621 ccttgtctaa catgataacc ccattatctt tataccgagc aaggtcataa cctgcattga
     1681 ccagttgatt gcgcactgta tttttagata tacccaactc atcagcaagg ttttttatgg
     1741 tcattttttc tggcaaatca gtcattatct tctccgttac tttcagctac cccataaaca
     1801 tatttaacag catgcttagg gataatgatt ggggaatcaa ttttattatt tttcaaaatc
     1861 aactcatact gcgttacatc aagtaactta ccggacattt ttttgctatc caaaaaaaca
     1921 acgacaacgt tatcacctgt taaattcacc aaccaatttt tcttaccaaa atgcttttgt
     1981 ttttctgcca ttactattcc tcctctgtca catcaagtcc ctgtaaccaa gtatccaatt
     2041 ctttttttaa atcatctgct gattcttcaa atgtttctcg taaatgattc gtcaactcat
     2101 cattttgact ttctcgcttt aataaatttc gatatttata tttcgagttt cttgacaaaa
     2161 tcgcccaatt agaatgatta gctaacaatc catcaatcat aactttatcc gtaggtttta
     2221 tatcagttgg taaaaaatgt ggactcacaa agctatccaa actttctttc accatagcat
     2281 accattccgt agctttacca cgtcttaact gcatttctaa ccgccaccaa gtattaattt
     2341 ctgaaggcac cacctttctt ttcatttctt gctctatttt tttattgtac aaacgaattt
     2401 gccgctcgct tgcccgacta ccccaataca ccgtttccag ttttcctgag cgtccataaa
     2461 ttaatttaaa tgaaactgga tcaataattc gatattgcgt gataaactca tcaggcacat
     2521 caataagatc gcaggcaaca tccgctcgtg aaaaatgggg ctctaaaaat aaatcatgaa
     2581 taaaattttt catggaatcc gctaaaaatc gactgatttt attaggatta aaatcaatcc
     2641 gcccttttcc ttcttgccat ttagaaattt caatataggc aatattttcg tcatgttggt
     2701 ctaatagctg ccaaccatta tttccaaccg cttttaaata gccattgcct tcatttaccc
     2761 gcataagctg ttcaaaatct aatattaata catcatgttg agtatgataa aaaatatttt
     2821 gattcaactt tccaacaatg gtaattcggt caatcgacca atttaatttt atagtttgta
     2881 ccaaagtttg aacatttaac cgcctgttag agggggctgt taatttttcc cactcattta
     2941 tgatcgtgcc ttttttactc aaattttgtt cctcctcaaa gaaaaagaga cttctggaaa
     3001 gaattttcaa aagttcacgt aactcttaaa aattcattac caaaaaatct ctagtcttga
     3061 agaaatctaa aaatattcgt ataatgatta agaagttacg gagctagtgc gattagtttc
     3121 cgtaaaggtc gagaacgtgt ccagcgttgc tcgactttat ttttttgctc ttttttcagt
     3181 ttatttattt gataactcaa taatagcact tctctttagc tatgcaaata tccaaaaaat
     3241 aaaaccgcac cctttgaaaa gggcgccccc aaacttcgct aacgctaggt tggccaaaaa
     3301 atggacttgc cattttttag caaatgtcag cttcagggat tagtggttta gccgtcaact
     3361 ccttatcctc ggcacaaacc gcctccggta ccgttgactg cacatcgtaa ctaccactcg
     3421 atctcatctt cagaacgctc taatttacgc tgtgagacgt tttgacgata aaataaggtt
     3481 ttctcctgct ctaacacttc taattcctca gaaagcccca tatccttttc tgcaagacca
     3541 aaacggatag acaaacgagt gatcgatttt tgaaatgcgt tttttaatcg ctctaaacgc
     3601 tggcgtaaag agcgattttc ttctttcagc tttttatttt ccacttgtaa atctgctaat
     3661 tgttttctac cagacaaatg acgctcttct tccaatgtag ctacatactc atttaattct
     3721 gtattttgct caagcatctg taaatattgc tcactcatgt tatcagcatt tctcttggca
     3781 tttgttgctc tcgtagctcg ttgatacaaa tcaacaaaat cattcccaga taaatgatac
     3841 gaaccatcag gaagcctctt gaaactttct aaaggaacaa tatctttttg gaaaatgacc
     3901 gccatctctt tttttacatt cagcatattt ttcttctctt cttcaatctt cactctctca
     3961 gaagccacca gagccttttc tcgctcattt tcttctcttt gcctagaaat atacctcaac
     4021 tcatcagaaa cgctctcacg ctcttttacg gccgtttgac gcaattctga aatctcgctc
     4081 atgatttctt tatattcgtg aa
//
LOCUS       DAZKKW010000144         4032 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.146, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000144 DAZKKW010000000
VERSION     DAZKKW010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4032
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.146"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..692
                     /locus_tag="ACG8PO_002703"
     CDS             <1..692
                     /locus_tag="ACG8PO_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HFQ0207960.1"
                     /translation="HEYKEIMSEISELRQTAVKERESVSDELRYICRQREENEREKAL
                     VASERAKIEEEKKNMLNVKKEMAVIFQKDMVPLESFKRLPDGSYHLSGNDFVDLYQRA
                     TRATNAKRNADNMSEQYLQMLEQNTELNEYVAALEEERHLSGRKQLADLQVENKKLKE
                     ENRSLRQRLERLKNAFQKSITRLSIRFGLAEKDMGLSEELEVLEQEKTLFYRQNVSQH
                     KLERSEDEIEW"
     gene            1281..2270
                     /locus_tag="ACG8PO_002704"
     CDS             1281..2270
                     /locus_tag="ACG8PO_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HFQ0207961.1"
                     /translation="MKKKKQDLINGTFREKKKNLKKVVAFSDNRISPKMRELMTGCGS
                     YLEFIATIDKEKKKLVQAHFCKNRFCPLCAWRKARKDSMMLSIMMQAIAEEKQYQFIF
                     MTLTTPNVKAECLNDEIDLFNHALKKMFMRKRVKTSIKGYVRKLEITYNKKRNDYNPH
                     FHLILAVNKSYFTDKDYYIKQSEWLDLWREVTGKTGINPDGTDEITQLDIRKVKGFQQ
                     EKAVQEVAKYSAKDFELTESQEVFDTFYHAMKGRQLITFNGVFKEYKKKYESGDLDRY
                     KKKDENEYFWFLTASWMRKESKYAVDYREMSADERNYFSGMYVDEKSVEGENE"
     gene            2267..2686
                     /locus_tag="ACG8PO_002705"
     CDS             2267..2686
                     /locus_tag="ACG8PO_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0207962.1"
                     /translation="MTDSTDKMTIKALADELGITKNTVRNYLVKAGYDLARYKKNGII
                     LLDKELIGVARSIYRSKGGQLTGHLPVNSTGSDFPLEKENEFLRDQLAEKDKHISELT
                     EMIRDQQKLTLLQQQKNDQLLLENQELKNRKWWQFWK"
     gene            2695..3003
                     /locus_tag="ACG8PO_002706"
     CDS             2695..3003
                     /locus_tag="ACG8PO_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0207963.1"
                     /translation="MDKIDLREETVNRIEVMYERLADYLNLSDEDNYSEMLERMMDVL
                     NSVINADLAKSSVLTNEFVHKHLAQTTDEAEKEFWLKLMSHIMQLNQISVINSKEKGM
                     "
     gene            3007..3369
                     /locus_tag="ACG8PO_002707"
     CDS             3007..3369
                     /locus_tag="ACG8PO_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0207964.1"
                     /translation="MGMTLADIKVQEEKLKKEQAKLRQQKKRLIEKENAAMGKALRKR
                     FGFESFADFERWLSSISDDQQHEKSVGNELRADEKSAPSERNYPNESDGIFGGIPNRK
                     SQMSESGFASMFSDVNSR"
     gene            3591..>4032
                     /locus_tag="ACG8PO_002708"
     CDS             3591..>4032
                     /locus_tag="ACG8PO_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="HFQ0207965.1"
                     /translation="MGFSISFKKGTKNTSISHNNRELTDSQKINDWHKHIDFTKSDEN
                     IYLEQINIREKYEELFGEAVEKYNAKQKRADRKIEDYLAKVRKDKKMEPQREFIVQIG
                     TLDDFRTTRDDGSSTGISEQQAEQNRVIANKILVEYFKEFKERNP"
BASE COUNT         1411 a          563 c          927 g         1131 t
ORIGIN      
        1 ttcatgaata caaagaaatc atgagcgaaa tttccgaatt gcgtcaaacg gccgtaaaag
       61 agcgtgagag cgtttctgat gagttgaggt atatttgtag gcaaagagaa gaaaacgagc
      121 gtgagaaggc tctggtggct tctgagagag cgaagattga agaagagaag aaaaatatgc
      181 tgaatgtaaa aaaagagatg gcggtcattt tccaaaaaga tatggttcct ttagaaagtt
      241 tcaagaggct tcctgatggt tcgtatcatt tatctgggaa tgattttgtt gatttgtatc
      301 aacgagctac gagagcaaca aatgccaaga gaaatgctga taacatgagt gagcaatatt
      361 tacagatgct tgagcaaaat acagaattaa atgagtatgt agctgcattg gaagaagagc
      421 gtcatttgtc tggtagaaaa caattagcag atttacaagt ggaaaataaa aagctgaaag
      481 aagaaaatcg ctctttacgc cagcgtttag agcgattaaa aaacgcattt caaaaatcga
      541 tcactcgttt gtctatccgt tttgggcttg cagaaaagga tatggggctt tctgaggaat
      601 tagaagtatt agagcaggag aaaaccttat tttatcgtca aaacgtctca cagcataaat
      661 tagagcgttc tgaagatgag atcgagtggt agttacgatg tgcagtcaac ggtaccggag
      721 gcggtttgtg ccgaggataa ggagttgacg gctaaaccac tagcctatta agctgacatt
      781 tgctaaaaaa tggcaagtcc attttttggc caacttagcg ttagcgaagt ttgggggcgc
      841 cctttttaaa gggtgcggtt ttatgtttgc atgactaaga atgagtggga aaatttaaca
      901 gccctatcta acaggcggtt aaatgtacaa aatatgtaca cccccttaaa aatgcagtgg
      961 tcgattgatc gaattttatg tctttgggtt ttggggtttt aaaacgaaaa aaagggggcg
     1021 attttcgccc tcttttttga tgttttttct tatcttgata ctataggcaa ctatttctgc
     1081 gaagaaatcg gggctatagt atgggtctat cccttgtggc tctaaggaag aacaaaaaaa
     1141 aagattgctt tttcttccct agtattttat agttttaatt gactagaaaa aaactaaact
     1201 agaaaggaga gcaatctatt ttttgttatc ggcttttcag ccttttttgt atacaaaatt
     1261 tgtagattca gtatagcaag atgaaaaaga aaaagcaaga tttgattaac ggaacatttc
     1321 gagaaaaaaa gaaaaatttg aaaaaagtgg tggcatttag tgataatcga atttctccca
     1381 aaatgcgtga attgatgaca ggctgtggtt catatttaga atttattgca acgattgata
     1441 aggagaaaaa gaaattggtc caagctcact tttgtaaaaa tcgtttctgt ccgttatgtg
     1501 cttggcgtaa agctcgtaag gattcgatga tgttgtcgat tatgatgcaa gctattgcgg
     1561 aagaaaagca gtatcaattt atttttatga cgttgacgac accgaatgta aaagcggaat
     1621 gtttgaacga tgaaattgat ttgtttaatc atgcattgaa aaagatgttt atgcgcaaac
     1681 gggttaagac ttcgattaaa ggctatgtgc gaaaattgga aatcacgtac aacaaaaaac
     1741 ggaatgacta taacccgcat tttcacttga ttttggcagt aaataaatct tatttcacgg
     1801 ataaagatta ttatatcaag caatcggaat ggttagattt gtggcgggaa gtgaccggaa
     1861 aaacgggaat taatccggat ggtaccgacg aaattaccca gttggatatt cgcaaggtca
     1921 aagggttcca acaagaaaaa gcagtacagg aagttgcgaa atattcggca aaagattttg
     1981 aactgaccga aagtcaggaa gtgtttgaca cattctatca tgcaatgaaa ggtcgtcagt
     2041 tgattacgtt taacggtgta ttcaaagaat acaagaaaaa atatgagtcg ggtgaccttg
     2101 atcgttataa gaaaaaagat gagaatgaat atttttggtt tttgactgct tcgtggatga
     2161 ggaaagaatc gaaatatgcg gttgattatc gtgagatgag tgctgatgaa agaaattatt
     2221 tttcaggaat gtatgtcgat gaaaaatctg ttgaggggga aaatgaatga ctgattcaac
     2281 ggataaaatg acaattaagg cgcttgctga tgaactagga ataaccaaaa atacagtgag
     2341 aaattatcta gtcaaagccg gatatgatct tgctcggtac aagaaaaatg gaattatctt
     2401 gctagacaaa gagttgattg gtgttgctcg gtcaatttat cggtcaaaag gtggtcaatt
     2461 gactggtcat ttaccggtca atagcacggg cagtgatttt cctttggaaa aagagaatga
     2521 gtttttgcgt gatcaattag ctgagaagga caaacatatt tctgaattaa cagaaatgat
     2581 tcgtgatcaa caaaaattga ccctgcttca gcaacaaaaa aacgatcagt tgttactaga
     2641 aaatcaagaa ttaaaaaatc gaaaatggtg gcaattctgg aagtgagatg tgacatggat
     2701 aaaattgatt tgagagaaga aacagtcaat agaattgaag tgatgtatga acggttagct
     2761 gattatttga atctttcgga tgaagataat tatagtgaga tgcttgaacg aatgatggat
     2821 gttctcaact cggttatcaa tgctgatcta gcaaaatctt cagttttgac taatgaattt
     2881 gttcataagc atttagcgca aacaactgat gaagctgaaa aagaattttg gttgaaatta
     2941 atgagtcata ttatgcagtt aaaccaaatt tccgttatta attccaaaga aaaggggatg
     3001 tagtttatgg gaatgacttt agcagatatt aaagtgcaag aagaaaaatt aaaaaaagaa
     3061 caggcgaaac ttcgccaaca gaagaaacga ttgattgaga aagaaaatgc tgcaatgggc
     3121 aaggctcttc gcaagcgttt tgggtttgaa tcttttgctg attttgagcg gtggctatcg
     3181 tcgatttctg acgatcagca acacgaaaaa tcagtgggta atgagttgcg agctgatgaa
     3241 aaatcagcac caagcgaacg gaattaccct aacgaatctg acggaatatt cggagggata
     3301 ccgaatagaa aaagtcagat gagtgagagt ggatttgcgt caatgtttag tgacgtaaat
     3361 tcacgataag cgaatatttt gcatgaagca aaataaagcg taaaaggagt tgagagcgtg
     3421 acacgctttc ggctcctttt ttcgttgtca cttgtgacgg cgaaaaccct cggagagcgt
     3481 ttggaattgt gacagtcatg tcacaattag gaaaacgtta ctctttgaca aagagtaaca
     3541 gaaaggttta cactcttttt attagcgaca gcgcgaagga ggggtttaac ttggggttta
     3601 gtatttcgtt caaaaaggga acaaagaata caagcatttc tcataataat cgtgagttaa
     3661 ctgatagcca aaaaatcaat gactggcata agcatattga tttcacaaaa tcggatgaaa
     3721 atatctattt ggaacaaata aatattcgtg aaaaatatga agaattattc ggtgaagccg
     3781 tagaaaaata taatgcaaag cagaaaagag ccgatagaaa aattgaggac tatttagcaa
     3841 aggttcgcaa ggataaaaaa atggaaccac agcgagaatt tatcgtgcaa attggtactc
     3901 ttgatgattt tcgtactact cgtgatgatg gttcttctac tggcatatcg gaacaacaag
     3961 cagaacaaaa tcgagtgatt gccaacaaaa ttttagttga gtatttcaaa gaatttaaag
     4021 aacggaatcc ta
//
LOCUS       DAZKKW010000145         4031 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.147, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000145 DAZKKW010000000
VERSION     DAZKKW010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4031
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.147"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            961..1929
                     /locus_tag="ACG8PO_002709"
     CDS             961..1929
                     /locus_tag="ACG8PO_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFQ0207966.1"
                     /translation="MSNEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKSDIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGFA
                     YFSKEDFHELLDIPKSYNQGTIDQRVFNPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKSDDFNKHAPTKEEQNKALELANQQVQSEKAPTSLQEKIKEQKRKLELL
                     QQLEKLEREQNTQNKENNAQKSEINSDIPSELKKDLLDNLQNLFKD"
     gene            1946..2470
                     /locus_tag="ACG8PO_002710"
     CDS             1946..2470
                     /locus_tag="ACG8PO_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFQ0207967.1"
                     /translation="MTEKTIKELAEELGVSKTAINKKVSDENRKLWFSKIGNKFVINE
                     TGQKAIKSMFFNENENSNQQLVGEKTKTENHKNANQVPELEVLSLLEQQLNQKDMQLQ
                     EKDEQIKQMQKLLDHQQQLTLQANQQIEKLQEQLQLTYTEESSENKSTTLSEETESIE
                     KQSKAEKKWWQFWQ"
     gene            2846..3127
                     /locus_tag="ACG8PO_002711"
     CDS             2846..3127
                     /locus_tag="ACG8PO_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000353845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0207968.1"
                     /translation="MDILVRNINPKIIARIDKRCQELTTKTGKKWSRNKYLKVLIESD
                     FDRQLMNYKKDQFDLLLEKFIAIQTENTNILNDYVHTNNQLIETLIGGI"
     gene            3129..3545
                     /locus_tag="ACG8PO_002712"
     CDS             3129..3545
                     /locus_tag="ACG8PO_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0207969.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDVNNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIMAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     ENQLNPSRRNQPKNRQKETNQTFTSGNFDPTNFLKR"
BASE COUNT         1538 a          713 c          670 g         1110 t
ORIGIN      
        1 cccttttagt gagttttgta gaaagtacct ttaaaggtac tttcacatta cgaacgatac
       61 gtccgtagga cgtactgaaa gggtgggact tatgagcaaa ttaatgcaaa atttggctag
      121 caaaaaaagt taaaaaatat atgctaataa tcgagcgaaa tgaacacttt ttcaagtgag
      181 cgaatccgcc atgacaaagc tgaagcagtt tatttcagct ttgtcatggc gtgtgcctag
      241 acttttaaaa aagtcggcac acgagcaaat atctttatca agctctaatt tgcgttttaa
      301 gacgtttgaa acgtctaagt atataaatat ataagagtag gtttaagagt gcttaaatcg
      361 aattttagag ggcattcaga caactttagg gttgttaggg cttgccctga ccgcctgtaa
      421 ggcgctttaa gaacaagata agggatttat actaagactg tcttaaaacg tctcaaacga
      481 aggctcatag cttctcaaag agttccttat gaacattttt agcaaaggag ccggaagcga
      541 ctgcgcagta taatgtgaga gcagagcgat cacacctttt taagcgactg tagggagtaa
      601 gattcccagt tgagaaaaat gcgcgaatct gtttggagaa aaaagaagtg gttttttagg
      661 cgggaaattt tgtccccctc ttttataatc tctttttaaa cctcttttta aacctcttta
      721 ccgtcggcta tatccttact ctcccaaggg aatgacgctt ttatctacat cgttttgtct
      781 gtttatctac atcgttttgt ctgtttatct acatcgtttt gtctgtttat ctacatcgtt
      841 ttgtctgttt gtacattgaa ttagtacatc gacgatgtta atataagatg tagataaaca
      901 ggcattttgt atttcataac gtgtatctct tgtgcgagta agaaaggagc tattttaaat
      961 atgtctaatg aaatcgtaaa gtatcatcat gaattaaata caattccgtt gcgaaaattt
     1021 acaccagtcg aaatgaattt gttcttttct atcgtatcaa gaatgcgtga tgtaggtgat
     1081 aaaaaagtac aatttacatt cgagcaatta aaagatttaa gcaattataa agcaactgca
     1141 aatgttcgat tcatagatga tttagaaaca acgtatgaca aattaatgga tttacgtttt
     1201 ggtagaagaa gtgctgacgg tttgcaacgt gaacgttttg ttttatttaa tcaatttaaa
     1261 atcgacggaa aatctgatat tccttttgct gaaatacaag ttcatgaaaa agccttaccc
     1321 ttacttaata atcttgaaga atgggtgcgc tattctctcc aacaattcaa tgaattagaa
     1381 agcagttatt caaaaacaat gtttcgatta ctaaaacaat acagaaccaa aggtttcgct
     1441 tatttttcaa aagaagactt tcatgaatta cttgatattc ctaaaagtta caaccaaggt
     1501 acgattgatc aacgtgtgtt caatccaata cgacaagaac taaccgcaat ctttaaaggt
     1561 ttaacaatca aaaagaaata cggaaaagga cggggaaagc ctgtcattgg ctatcaattt
     1621 acatttaaac ctgagataaa aaaatcagac gactttaaca aacatgcccc aacaaaagaa
     1681 gaacaaaaca aagcattaga actagctaac caacaagttc aatctgaaaa agcacctact
     1741 tcattacaag aaaaaatcaa agaacaaaag cgaaaattgg aacttttaca acagttagaa
     1801 aagttagaaa gagaacaaaa tacccaaaat aaagaaaata atgcacaaaa atcagagata
     1861 aattccgata ttccttcaga actaaaaaaa gacttattag acaatcttca aaacttattc
     1921 aaagattgag gggtttttag attttatgac agaaaaaaca atcaaagaac ttgctgaaga
     1981 attaggagtt agtaaaacag caattaacaa aaaagtttct gacgaaaatc gaaaactttg
     2041 gttttcgaaa atcggaaaca agtttgtgat taatgaaacc ggacaaaagg ctataaaatc
     2101 aatgtttttt aacgaaaacg aaaactcaaa tcaacaacta gttggcgaaa aaacgaaaac
     2161 cgaaaaccac aaaaacgcca accaagttcc cgaattagaa gtcctttctc tcctagaaca
     2221 acagttaaat caaaaagata tgcaactaca agaaaaagac gagcaaataa aacaaatgca
     2281 aaaattactc gaccaccaac aacaactcac cctgcaagcc aaccaacaaa tcgagaaact
     2341 gcaggaacaa ctacagttga cttatacgga agaatcttcg gaaaataaat caacgactct
     2401 ttctgaagaa acagaatcga tagaaaaaca atcaaaggcg gagaaaaaat ggtggcaatt
     2461 ctggcaataa aaccagcaat ttaaattaat aaaaattgct ggttttattc ttctttttgc
     2521 ttttgcgagc agagcaacat ggacccatgt tgcgatattt tggtggcgcc ccaatccccg
     2581 ttttttcgtt gaatcttact cggactgcac tcgttttaca gctgattttt ctgaattttg
     2641 aaagagcata aggaaacgtg gtatcacttt cttacgaaag gtgctagata ggctgtgcct
     2701 atcacgtttc tcagaaaaaa atttgctatc acttttttgc cattgtggct aggctatgta
     2761 tagtgttttg gcgaaaaata gctatacaaa tactatattt gtatggtata cataaattat
     2821 acgctgatac gaaaggaaaa tttccatgga cattttagtt cgaaatatca atcccaaaat
     2881 tattgcaaga atagataaac gttgtcagga actcacaacc aaaacgggca aaaaatggag
     2941 tcgaaataaa tatctaaaag ttctaatcga aagtgatttt gaccgtcaac ttatgaatta
     3001 caaaaaagat caattcgatt tattactcga aaaatttatt gccattcaaa cagaaaatac
     3061 gaacatactg aatgactatg ttcacacaaa caaccaactc attgaaacac ttatcggagg
     3121 aatataacat gagaaaaact tctgttcaaa aacacgtaac cctttatccc agtcacgttg
     3181 acgttttaga ttccatcatc gaaaaaaatc cagacgtcaa caattattca gaagccattc
     3241 gttttttatg tatgagttat gaacctgata ccaatacaac cacaaccagt aaccaagcaa
     3301 aactcaatgc catgggaaaa gaaatttcta tcatgacaga aatcatggca gaatttgctg
     3361 atttatatct aaaagatact ggtgttctta tcggtacaaa ttctaaagtg tatcaagaag
     3421 tgaaagaaaa aattgaaaat caactcaatc cttcccgacg aaatcaaccc aaaaatcgtc
     3481 aaaaagaaac caatcaaacc tttacttctg gaaattttga cccaacaaac tttctaaaaa
     3541 gataaaaaaa gaaaccgatc cctaaaaaaa ttcctcgatt tctttttagg gatcagtctt
     3601 ccagcaagat ttgcaagttg gtaccaactt gcgaaaaaaa agaaaaatcc gcttatgcaa
     3661 cttcttcttt ttgtattttg aggcgctacc ccaatcccca agtatcggtt atttaatagc
     3721 gcggaaatcc aaagaatagg acgtaccctg aaacaaaaaa agactgctct cacgagcagc
     3781 ccaaaatgag attatcctat tcatcaacat aaaaatatta caaagtgaag aactagagaa
     3841 agacaaaaaa agcatatcaa atcctctcaa aaataacaag tctttaaaaa atgacagata
     3901 aaaatgcaca aagaactaaa aaagagaaat cgcgtaaaat caagcattag aagctagtaa
     3961 ctagctagac caattaaaaa tcacaaagtg agcgtctcta tccattggta gaggggctac
     4021 ttggcatagt g
//
LOCUS       DAZKKW010000146         3931 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.148, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000146 DAZKKW010000000
VERSION     DAZKKW010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3931
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.148"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..585
                     /locus_tag="ACG8PO_002713"
     CDS             <1..585
                     /locus_tag="ACG8PO_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0207970.1"
                     /translation="FPDYYNDAQGIVLMNFINPILVLIVVSPLTLFLSKKYGKKELAS
                     VGMFFSAIVYLILFLIKASNMYVFLILSSVGYMGLGVFNTVIWANITDVIDDQEVKSD
                     QREDGTVYAVYSFARKIGQALAGGVGGWTLSIIGYDSLANVQTGDVLEKLYNASTLIP
                     SICFLIVGLILFFLYPLSKDKVLENCELLKQKHK"
     gene            595..1389
                     /locus_tag="ACG8PO_002714"
     CDS             595..1389
                     /locus_tag="ACG8PO_002714"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFQ0207971.1"
                     /translation="MNNWIFCIDSDGCVMDTMTYKHNLFFGPIAAEIFDVHHREKFLQ
                     DWNNINLYSETRGINRFLGLVKTLEKNDVTSINRLKHWVSLTKELSNPSLKREIEYSQ
                     SEDLLKALKWSEEVNQQISQAKGLDKPFLSARDTIKALKKYGKIIIVSSANKEAVQEE
                     WERHELLSLVDELCCQDKGKKEDIIRSVIENGCDLDKILMIGDSPGDLEAANKNKVFF
                     YPILVNKEKESWENLRTDVLFKCLSGDYVDIEQKYIDTFWKNLTNK"
     gene            1402..3168
                     /locus_tag="ACG8PO_002715"
     CDS             1402..3168
                     /locus_tag="ACG8PO_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HFQ0207972.1"
                     /translation="MTIKVDLRKKPYNLDEDAISWIEETIDSMTLEEKIGQLFVNMGS
                     SRDKNYLSNMVNQYHIGAVRYNPGTADEVYDQNYILQTESKIPLLIAANTEAGGNGAC
                     TDGTEVGVEVKIGATNDAKYAYELGRVSGIEASAIGCNWSFAPIVDINRNWRNPIISS
                     RSFGQNPDKVLELSLAYMKGIQESGILPAAKHFPGDGIDERDQHLSFSINSLGTEEWD
                     NSFGKVYSGLIEAGLPSIMAGHIHMPAYSKHFNPDLKDEDLLPATLSKELITDLLRGK
                     LGFNGLVVTDASHMLGMTGAMKRSELLPTSIAAGCDLFLFFNDPDEDFNYMMEGYRKG
                     IITDDRLLDALKRILGLKAKLGLHKKAKEQILLPKQEALEKIGLIENKSIFKEVADQA
                     ITLVKDKQDIWPIVPEKYKRILLVDVHGVKGGFGEVVAGNRPQAIDLLKQILSAKGFE
                     VEKYISPEEELKGLSQEEKIKNIAGIYAAKRPIKNLTKKYDLIINIANVNPGTVQRIV
                     WPASKGTPDIPFYIHEIPTIFVSVQCPFHLADVPQVKTYINTYDGKQETLEILVDKLM
                     GLSEFKGVSPIDAYCGLKDTFI"
     gene            3191..>3931
                     /locus_tag="ACG8PO_002716"
     CDS             3191..>3931
                     /locus_tag="ACG8PO_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding domain-containing protein"
                     /protein_id="HFQ0207973.1"
                     /translation="MLYPIMTETRQVIDLSGLWRFKLDTNDDLQEELALEPINEKESY
                     PISVPSSYNDLFETENIRNHVGWVWYEKEVTLPKRLLEERLVLRFGSATHEAKVFLNG
                     KFITQHKGGFTPFESEINRFVKDGNNRITVAVNNIIDDSTLPVGIMKETVQENGDIKK
                     ENLVNFDFFNYAGIHRPVKLYTTPKDYISDITINTRITDLDASVEFNIDTEWTGKILI
                     AIYDEEAQLVAEAEGKKGMIQLKNPIFWE"
BASE COUNT         1421 a          466 c          779 g         1265 t
ORIGIN      
        1 tttcctgatt actataatga tgcacaggga atagttttaa tgaattttat caatcctatt
       61 ttggttttaa tagttgtttc gcctttaact ttgtttttaa gtaaaaaata tggaaaaaaa
      121 gaactggcat cagtaggaat gttcttttca gcaatagtat atttgatctt atttttgata
      181 aaagctagta atatgtatgt attcttaatt cttagttcgg ttggctatat gggtttgggt
      241 gtgtttaata ctgttatttg ggcaaatatt actgatgtta ttgatgatca agaagtaaag
      301 tctgatcaaa gagaagatgg gaccgtatat gcggtatatt cttttgctag aaaaataggt
      361 caagctctag ctggaggcgt cggaggttgg actctttcta ttataggcta tgactcttta
      421 gcaaatgtac aaacgggaga tgttttagaa aagttatata acgcttcaac attaattcca
      481 tcaatttgtt ttctgattgt aggattaatt ttatttttcc tttatccatt atctaaagat
      541 aaagtcttag aaaattgcga gcttttaaaa caaaaacata aataggagag aaatatgaat
      601 aattggatat tttgtattga ttcagatggc tgtgttatgg atacgatgac ttataaacac
      661 aatctctttt tcggaccaat tgctgcagag atttttgatg ttcatcatag agaaaaattc
      721 ctacaggatt ggaataatat taatttatat tcagagactc gtggaataaa cagattttta
      781 ggtttagtta aaacattaga aaaaaatgat gtaacatcga ttaatcgatt aaagcactgg
      841 gtatctctaa caaaagaatt gtctaatcca tctttgaaga gggaaataga atattctcag
      901 tcagaggatt tattaaaagc tttaaaatgg tcggaagaag tcaatcaaca gattagtcaa
      961 gcaaaaggat tagataaacc ctttttaagt gctagagata ctataaaagc tctgaaaaaa
     1021 tatgggaaga taattattgt aagctcagct aataaggaag ctgttcaaga agaatgggaa
     1081 agacatgaat tattatcatt agttgatgaa ctgtgttgtc aagataaagg aaaaaaagaa
     1141 gatattattc gaagtgtaat tgaaaatggt tgtgatttgg ataagatatt aatgattggt
     1201 gattctcctg gtgatctaga agctgcaaat aaaaataaag ttttctttta tcctattttg
     1261 gtcaataaag aaaaagaatc atgggaaaac ttaagaactg atgttttatt taaatgttta
     1321 tcgggagatt atgtagatat tgaacaaaaa tatatagata ctttttggaa aaatcttact
     1381 aataagtaga ggagaaagaa aatgactata aaagtagatt tacgaaaaaa accatataat
     1441 ttagatgaag atgctatttc ttggattgaa gagacgattg attctatgac tttggaagaa
     1501 aaaataggtc aattgtttgt taatatggga tctagtcgtg ataaaaatta cttgagtaat
     1561 atggtcaatc agtaccatat tggagctgta cgatataatc caggaactgc tgatgaggta
     1621 tatgatcaaa actatatctt acaaacagaa agtaaaattc ctctattaat tgctgctaat
     1681 acggaagcag gtggtaacgg agcttgtaca gatggtactg aagttggtgt agaagtaaaa
     1741 attggtgcaa caaatgatgc gaaatatgct tatgaattag ggagagtttc tgggattgaa
     1801 gcatcggcta ttggatgtaa ctggagtttt gctcctattg tagatattaa tagaaattgg
     1861 agaaatccta tcatttctag tcgatctttt ggacaaaacc ctgataaagt tttagaactt
     1921 agtttagctt atatgaaagg tattcaggag agtggaattt tgcctgcagc taaacatttc
     1981 cctggtgacg gtattgacga acgagatcag catctatctt ttagtattaa tagtttaggt
     2041 acggaagaat gggataattc ttttggaaaa gtatactcag gcttaataga agctggatta
     2101 ccttcaatta tggcaggtca tatccatatg ccagcttatt caaaacattt taatcctgat
     2161 ctgaaagatg aagatttact tcctgcaact ttatcaaaag aattaatcac tgacttactt
     2221 agaggaaaac tcggatttaa tggattagta gttactgatg ctagtcatat gttaggaatg
     2281 actggggcaa tgaaacgatc agaattattg cccacttcta ttgcagctgg atgtgatcta
     2341 tttctatttt ttaatgatcc agatgaagac tttaattata tgatggaagg ctatcgtaaa
     2401 ggaattatca ctgatgatcg gttattagat gccttaaaaa gaattttagg gttaaaagct
     2461 aaattgggat tacacaaaaa agcaaaagaa caaattttat tacctaagca agaagctctg
     2521 gaaaaaattg gtttaattga aaataaatca atttttaaag aggtggctga ccaagcaatt
     2581 acattagtta aagataaaca agatatttgg cctattgtac cagaaaaata taaaaggatt
     2641 ttactagtag atgtacatgg ggtaaaaggt gggtttggtg aagtagttgc tggaaataga
     2701 cctcaagcca ttgatttatt gaaacagatt ttgtcagcaa aaggatttga agttgaaaaa
     2761 tacatttcac ctgaagaaga attgaaagga ttatcgcaag aagaaaaaat taaaaatata
     2821 gctggaatat atgctgctaa gagaccaatt aaaaatttaa ctaaaaaata tgacttgatc
     2881 attaatatag ctaatgttaa tccaggcaca gttcaacgaa ttgtctggcc agcaagtaaa
     2941 ggaactccgg atataccgtt ttacattcat gaaattccaa caatttttgt atctgtacag
     3001 tgtccgtttc atttggctga tgtacctcaa gtaaagactt atattaatac ttatgatgga
     3061 aaacaagaaa ctttagaaat attggtggac aaactaatgg gtctttctga atttaaggga
     3121 gtaagtccaa tagatgctta ttgtggttta aaggatacat ttatttaata tttagatagg
     3181 agtggtttat atgttgtatc ccattatgac agagacgagg caagtaattg acttaagcgg
     3241 tctctggaga tttaaattag atacaaatga tgacttacag gaagaacttg ctttagaacc
     3301 aataaatgaa aaagagagtt atccgatttc agttccctct tcttataatg atttatttga
     3361 aacagagaac attagaaatc atgttggatg ggtgtggtat gaaaaagaag tgactcttcc
     3421 taaaagatta ttagaagaaa gattagttct tcgttttgga tctgcaacac atgaagcaaa
     3481 agtttttttg aatggaaagt ttattactca acacaaaggt gggtttacac catttgagtc
     3541 tgaaattaat cggtttgtaa aagacgggaa taatcgaatt acagttgctg taaacaatat
     3601 tattgacgat agtactttac ctgtggggat tatgaaggaa acagtacagg aaaatgggga
     3661 tataaaaaag gaaaacttag ttaattttga tttcttcaat tatgcaggta ttcatagacc
     3721 tgttaagtta tatacaactc cgaaagacta tataagtgat attactatta atacaagaat
     3781 aactgatttg gatgcaagtg tagaattcaa tatcgataca gaatggaccg gaaaaatctt
     3841 aatagcgata tatgacgaag aggctcaact tgtagctgaa gctgaaggaa agaaaggaat
     3901 gattcagctt aagaatccaa ttttttggga a
//
LOCUS       DAZKKW010000147         3927 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.149, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000147 DAZKKW010000000
VERSION     DAZKKW010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3927
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.149"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            174..410
                     /locus_tag="ACG8PO_002717"
     CDS             174..410
                     /locus_tag="ACG8PO_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HFQ0207974.1"
                     /translation="MFKHAKCKGTKYCGNAQTVGGGNNAWGKLGQVVGGLTTGAVGGA
                     GLGTAICGPACGVVGGLYGAVAGGAAAGWDARKK"
     gene            429..644
                     /locus_tag="ACG8PO_002718"
     CDS             429..644
                     /locus_tag="ACG8PO_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042742 - defense response to bacterium
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Blp family class II bacteriocin"
                     /protein_id="HFQ0207975.1"
                     /translation="MHIKNTKTTFILSSEELKNIQGGSAVGVLGTTFSGATAGVKLCS
                     AGGPYAIAACGVGGALLGAGFSMWTGA"
     gene            complement(1347..2099)
                     /locus_tag="ACG8PO_002719"
     CDS             complement(1347..2099)
                     /locus_tag="ACG8PO_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFQ0207976.1"
                     /translation="MSYPIILCEDQIIQLNQLERIIDNFILFHDKVFKIVLKTQSPLE
                     VKKYLKQFRPKQGIYFLDIDLNHEVNGIELAEVIRKYDVQAKIIFTTTHDEMLPVTIK
                     RRVETLGFVTKDQTLDEYRNEIVELLLLAQERIDATKQVTNQAFVFSIGSQTFTFDIN
                     DIYFVEASKLPHRLSLCTKDGQYEFYGRISELEKKYPMLTRISRACLVNIFNVKEIDF
                     KKRSLYFDSELARNFTLGKAQKIKEKLKESTV"
     gene            complement(2114..3361)
                     /locus_tag="ACG8PO_002720"
     CDS             complement(2114..3361)
                     /locus_tag="ACG8PO_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFQ0207977.1"
                     /translation="MFVGNFFFFLLFYMTNTDLNKRKYLFVCLLIPLTFFVELFTDVS
                     AAIPIIGSYFILKEKKKTNIVLLNKLLLCMLIDDFRSIITSMVMTYMFSFEGIKGYGY
                     VVFQIMLDSLFLLMFIWLYRKLAINRIVEQSSSKLTAIFMIYLLTIGLFVSYVAHQYQ
                     VFDHFVFGVLIFLIIQMFVVLFLFIRITIRQREQYEQQLKRKELVYLKKYTDSLEKDQ
                     EKFARFRHDYKNLLLSLKEIANQNHDSILEKEIKELEIYSNSYLTNKFEYRYLKNIKN
                     EYLKSLLIAKLYQANNNKICCKFECPSTIIEVPVPIFDCIRVLGIILDNAIEASIESQ
                     DRELSLAIYRDDKQIEISISNSCREILLSIDTLMKKGITTKKGHQGLGLSSIEEINKK
                     NPNMFVNYQNDPYQFTTQVILLF"
     gene            complement(3437..3583)
                     /locus_tag="ACG8PO_002721"
     CDS             complement(3437..3583)
                     /locus_tag="ACG8PO_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EntF family bacteriocin induction factor"
                     /protein_id="HFQ0207978.1"
                     /translation="MEEKNRLNAKQCSDQELKKIKGGAGTKPQGKPASNLVECVFSLF
                     KKCN"
     gene            complement(3687..>3927)
                     /locus_tag="ACG8PO_002722"
     CDS             complement(3687..>3927)
                     /locus_tag="ACG8PO_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFQ0207979.1"
                     /translation="SDILEVLQKVYLKLEKQKYELDPGPLINRLVNYLYFTAYTNKIR
                     FTEYQEELIRNLSEIGRTAGINGLYRADYGDKSQF"
BASE COUNT         1344 a          619 c          560 g         1404 t
ORIGIN      
        1 tccacaaaaa ctttgaaaac agttcttgaa cactaacttt ttcattcctg aatcaaattt
       61 attttggaaa ttttactatt caagaataaa cttgataagt ttcaggaaga tttttactgt
      121 taatcaaata cgtatgttac agtagcaccg taaaaaatat tatttaaaga agcgtgttta
      181 aacatgcaaa atgtaaagga actaagtatt gtggaaatgc acaaactgtt ggtggaggca
      241 ataatgcttg gggaaaattg gggcaagttg taggtggact taccacaggg gcagtaggtg
      301 gtgcaggttt aggaacagca atttgcggac cagcttgcgg tgttgttgga gggttatatg
      361 gagctgtcgc tggaggtgct gcagctggct gggatgctcg aaaaaaataa aaaaggagaa
      421 taagtagtat gcatattaaa aacacaaaaa caacatttat attatcttca gaagagttga
      481 aaaatattca aggtgggagt gccgtgggag ttttaggcac tactttttct ggggcaacag
      541 caggagtaaa actttgttca gccggaggac cttatgcaat agctgcttgt ggtgtaggtg
      601 gagctttatt aggtgctgga ttcagcatgt ggaccggtgc gtaaacaatt aaaaaatatc
      661 aaacctcaag attggtttgt gtcgattacg aacataggta tgttctttat cttttcaaaa
      721 gtttggaatg gacagaactt tttactaaag cttcttgtgc tagtttttat aattatatta
      781 aatttttatc ttttattaaa gtcagataaa aattctttgg aaatgtaatg gtagagatat
      841 ttttacaaaa ggacttgcag cgctgtagtt ggtgctgcag gaggacctat ttcggccgtt
      901 acaggaggta ttggtaatgt acttgtcgga tgtgcattaa agcctgttcc tgcaagttaa
      961 atttataaaa acaggaaagt aattatctca aatcctatca agtacaatca taggattgca
     1021 cttgacagga caaatttttt aactaaaaaa tgaaaaacac tttctaaaaa ttagtagaat
     1081 ttgccaaatc tttcgcatct tccaccatac atttaatttt aattcccctt tatggtactt
     1141 aataaaaaca agaaaagaac tattcatttg gccctttttt ggttccagac catagaaata
     1201 ttcctccagt aactaatatt gctgttatgc acgtaactaa taacagctat aaaaattggt
     1261 acaactttaa taggattcag aaaaatttgc actagtgaag gaatctattt tattagaaaa
     1321 atgcaatctc cctaaagaaa aaaccactaa acagtgcttt ctttcaactt ctccttaatt
     1381 ttttgtgctt tccctaaagt aaagtttctg gccaactctg aatcaaaata tagtgaacgt
     1441 tttttaaaat ctatttcttt aacgttaaag atatttacta aacacgctcg actaattctt
     1501 gttaacatag gatatttctt ttcaagctca ctaattcttc cataaaattc gtactgccca
     1561 tctttagtac acaatgataa tctatgtggt aattttgaag cttctacaaa ataaatgtca
     1621 tttatatcaa aagtaaatgt ttgagaacct atggaaaaaa caaatgcttg attcgtaacc
     1681 tgttttgttg catctatcct ttcttgcgct aataacaata actcaacaat ctcgtttcga
     1741 tactcatcta gtgtttgatc ttttgttaca aatcctaacg tttcaactct tctttttatt
     1801 gttacgggta acatctcatc atgagtagtt gtaaaaatga tttttgcttg aacatcatat
     1861 tttctgatta cttctgctaa ttctataccg ttaacttcat gatttaaatc aatatctaag
     1921 aaatatattc cttgctttgg tcggaattgt ttgaggtatt ttttaacttc taacggactt
     1981 tgtgttttca atactatctt aaatacttta tcgtgaaaaa gaatgaaatt atcaataatt
     2041 cgttctaact gatttaattg tataatttga tcttcacata atattattgg ataactcata
     2101 aatcctcctt ccctcaaaac aacaaaataa cttgagtagt aaattgatac ggatcgtttt
     2161 gatagttcac aaacatattg ggattctttt tattgatttc ctctatacta gataaaccta
     2221 acccttgatg tccctttttt gttgttattc cttttttcat tagagtatct atagagagta
     2281 gtatctctct acaactattc gatatcgaaa tttctatctg tttatcatct cggtaaatag
     2341 ctagagacag ttctctatct tgactctcta tagatgcttc aattgcatta tcgagaataa
     2401 ttccaagcac cctaatacag tcaaatattg gaacaggaac ttcaataata gtacttggac
     2461 attcgaattt acaacaaatc ttattgttat ttgcttggta tagcttcgct atcaacaaac
     2521 ttttcaaata ttcatttttt atatttttta aataacgata ttcaaactta ttggttagat
     2581 aagaattaga atagatctct aattctttta tttctttttc caatatcgaa tcatggtttt
     2641 gattagcgat ttcttttaaa ctcagcaata aatttttata gtcatgtcta aatctggcaa
     2701 atttttcttg atctttttcc agtgaatcag tatatttttt tagataaacc aattcttttc
     2761 ttttcaattg ctgttcatat tgttctcgtt gcctaatagt tattctaata aataaaaata
     2821 gaacaacaaa catttgaatg attaaaaata ttaagacacc aaacacaaaa tgatcaaata
     2881 cttgatattg atgagcaaca taagaaacaa ataaaccaat tgtaagtaaa taaatcatga
     2941 atatagcagt aagtttagaa ctggactgtt caacaatcct atttatagct aattttctat
     3001 ataaccaaat aaacatcagc aaaaacaaag aatcaagcat tatctggaat actacataac
     3061 catacccttt tatcccttca aatgaaaaca tatacgtcat aaccatggaa gtaataatag
     3121 atctgaaatc atcaataagc atacataata gtaatttatt taaaagcact atattagttt
     3181 tcttcttttc ctttaatata aaataacttc cgattatagg tatagctgcg gaaacatcag
     3241 taaataattc aacaaaaaaa gtgagtggta tcaataaaca tacaaaaaga tattttcttt
     3301 tgttaagatc agtattagtc atataaaata agagaaaaaa gaaaaagttt cccacgaaca
     3361 ttcttaatag ataagctttt gtatcgatag taatcaagag atcccctcct gattttagga
     3421 tatcataaaa aaaattttag ttacactttt taaataatga aaaaacacat tccactaaat
     3481 ttgatgccgg ttttccttga ggttttgttc cagcacctcc tttaattttt ttaagctctt
     3541 gatctgaaca ttgtttagca tttagtctat ttttttcttc cattacgatt ccccctacaa
     3601 actataaata cttttatgtc actgattcaa tatacctatt tttctaatca aaaaattttg
     3661 agacatattt aatattataa gtatcattaa aattgagatt tatctccata atctgctcga
     3721 tataaaccat ttattccagc agttcttcca atttcactca aatttcttat taattcctct
     3781 tgatattcag tgaatcttat tttattagta taagcagtaa aatatagata attcaccaat
     3841 ctatttatta aaggaccggg atctaattca tatttttgtt tttctaattt taaatatacc
     3901 ttttgtaaaa cctcaagaat atcagaa
//
LOCUS       DAZKKW010000148         3913 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.150, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000148 DAZKKW010000000
VERSION     DAZKKW010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3913
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.150"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            93..1019
                     /gene="cysK"
                     /locus_tag="ACG8PO_002723"
     CDS             93..1019
                     /gene="cysK"
                     /locus_tag="ACG8PO_002723"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFQ0207980.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            1255..1701
                     /locus_tag="ACG8PO_002724"
     CDS             1255..1701
                     /locus_tag="ACG8PO_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFQ0207981.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            1973..3325
                     /locus_tag="ACG8PO_002725"
     CDS             1973..3325
                     /locus_tag="ACG8PO_002725"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidase"
                     /protein_id="HFQ0207982.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSDPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQEALGEIETAQ
                     "
     gene            3496..3783
                     /locus_tag="ACG8PO_002726"
     CDS             3496..3783
                     /locus_tag="ACG8PO_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFQ0207983.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
BASE COUNT         1365 a          631 c          820 g         1097 t
ORIGIN      
        1 ttttttttgc tagtcaaaga cgaatcactc gccataaatg aacagattgt attatgataa
       61 gtaagaagag caaagacaag gaggaattga agatgacagc tatctataac tcagtcaccg
      121 aattgattgg caaaacgcca atcgtcaaat taaataaaat cgtaccagaa gattcagcag
      181 atgtttttgt aaagcttgag ttttttaatc caggaggaag tgtaaaagat cgtattgctt
      241 taagtatgat agaaaaagca gaacacgatg gtctactgaa accaggtgat actatcattg
      301 aacctacttc tggaaataca ggaatcggat tgtcgatggt aggagttgca aaaggatata
      361 aagtaatcat cgtgatgcct gagacgatga gtatcgaaag acgtttattg atgaaagggt
      421 acggcgcaga gttgattttg actcctggtg cggatggtat ttctggatca atcagagaag
      481 cagaacgttt ggcaaaagaa aacggctact ttttaccatt acaatttgaa aatgaagcaa
      541 atcctcttgt gcatgagaag acgactggtc ctgagataca tcaagcattt ggagtaaatg
      601 gcttggacgc gtttgttgca ggtatcggta caggaggaac gatcactggc gctggccgag
      661 aattaaaacg tgtgtatccg aaaatcgaat taatcggggt agaaccagca gaatctgcta
      721 ttttagaagg gaaagaagcg ggacctcaca aaatccaagg aatcggtaca ggttttgttc
      781 ctaaaacact ggatacttct gtgtatgata aagtcctttc tattagtgga gacgaagcga
      841 tggaaacagc tagagaagtt gggcgaaaag aagggatttt agtaggcatc tcatctggcg
      901 ctgcaattgc tgccgcgcta aaagtcgcaa aagaattagg aaaaggcaag aaggtattag
      961 ctgtggttcc cgacaatggt gaacgttatt tatctacagc cctttaccaa gagatataag
     1021 gcacttggtt tttctccaga taggtttatg taaaaaactt ttaaagatga aaagaacaca
     1081 caaaaattta tgatgagaaa aattatttga atagagacgt aaaatagtga acgcgactgt
     1141 tttacgtctt ttttcattaa aatacaataa aattgaattt tcttttcatg ttaagtggat
     1201 tttaaaatgt aaaaatagta tgataagacg agaataacaa cgtggggtgt ttacatggaa
     1261 aataagttag tcgaagaggt tatcgaaaag ttaaaaaaag agaacattcg aatcacacct
     1321 caacggtacg cggttttaga atatttgatt gaacatcatt cccatccaac ggctgatgag
     1381 atttataaag cacttgaaca tcggtttcca aatatgagtg ttgcgactgt ttacaataac
     1441 cttcgcttat ttacttcgat tggatttgtt caagaaatga agtacggcga tgcttctagc
     1501 cgatttgatt tcagttcaaa gcgtcattat catgtaattt gtgaaaactg cggtaagatt
     1561 gttgattttc attacccagg gcttgaagat atagaaatgg ctgcgagcag actgactgat
     1621 tttgagatta atgaacatcg gttggaattg tatggactct gtccggattg taaagagtca
     1681 gcaaaaaagg cgcaaatgta aaaacagttt ctataagtgt tatacctaaa aaaagctagc
     1741 tgtgtaaaaa aacgctagct tttttatttt tttgtctttt attcacaatc ttagatatca
     1801 aagtgaatat tatcacaata agaatcttgt agatattggg ttcttatgct tgaaaaggaa
     1861 tgaaactaca aacaaacctt tgtgaattca caagcattaa actgttgtat ccgttttatt
     1921 tctgttatag tagttttgtc gaaggacaaa atgttaatcg gagggaccat aaatgaaagt
     1981 agtagtaatt ggctgtactc atgcaggtac tgcagctgta aaaagtattt taaaaaatca
     2041 tccagaagca gaagtgacag tatatgaaag aaatgacaat atttctttct tatcttgcgg
     2101 tatcgcactt tatgtcggcg gagtagtaaa ggacccagca ggcttgttct actcaaatcc
     2161 tgaagaatta tcttcattag gagcaacagt taaaatggaa cacgatgtaa cagacgtcga
     2221 tacagaaaat aaaacagtaa cggcaaaaga tctgaaaaca ggagaaacag aaacggtttc
     2281 ttatgataaa ttagtgatga caactggttc atggcctatt atcccaccga ttagtggaat
     2341 cgatgcgaaa aatgtactcc tatgcaaaaa ctacaatcaa gcaaatgaaa ttattgcaca
     2401 agcgaaagaa gcaaaacgtg tagtaattgt tggtggtgga tatatcggta ttgaattagt
     2461 agaagctttt gtcgaatctg gaaaacaagt gacattgatt gatggattag atcgcatctt
     2521 gaataaatat ctagataaac cattcacaga tatcttagaa aaagaattga cagaccgcgg
     2581 tgttactctt gcgctaggcg aaaatgttac tgaatttatc acagatgaag aaggaaaagt
     2641 gaaacaagta gctacaccaa cagatacatt tgatacagat atggtcattt tatgtgtggg
     2701 cttccgtcca aatacaaaac tgatcgaagg aaaagtagat acactgccaa acggggcaat
     2761 caaagtgaat gaatacatgc agacaagtga tccagacatt tttgcagctg gagactctgc
     2821 agtagtcaac tacaatccaa gtggcacgca aaactatatt cctttggcaa caaatgccgt
     2881 tcgtcaaggt ttacttgtgg gaaataactt gacagatcat aaaatggcgt atcgtggtac
     2941 tcaaggaact tcaggacttt acctatttgg ctggacgatc ggttcgacag gtgtgaccaa
     3001 agaaagcgca actttgaacg atctagaagt ccaagcgact gtatttgaag ataattatcg
     3061 tccagagttc atgccaacta ctgaaaaagt aatgatggag ctagtctatg aaaaaggaac
     3121 gaatcgtatc gtaggtgctc agttcatgtc caaatacgac attacacaat cagcaaatac
     3181 catgtcttta gctgttcaaa ataaaatgac tgtagaagat ctcgcacttt cagatttctt
     3241 cttccaacca cattttgacc gcccatggaa ttacttgaac ctacttgcac aagaagcgct
     3301 aggcgaaatc gaaactgcac aataaagaaa aagggttgtg acacaatttt gtcacgaccc
     3361 tctgtctttt tttgtgaaaa gtacaattag ttgtctttct tcgaattttt tgtcacaata
     3421 agcagcaaag ataaagctgt tacataaaag aacaagcagc tttatttgat tttgaaaaga
     3481 aaggtgagaa agaagatgaa taaagaatat tttgtccaac aagatgcatt tttagaagat
     3541 tataaaagtg cttcgaagaa attaacgatc aatattgatg agttggcaga tcaagtactc
     3601 tctcagttga atgaaacgca agaggaatat tacacgatgc cagcaaatga cgccaaagat
     3661 aatgtttctc atcgttttcc tttcagtaga cgcatctact cagatgaaaa tgacggcaca
     3721 ttgaatacag agtacacata tgaaggtcag ccatatgtgc tagaaactga tgatgatctt
     3781 taaagacagt ttttgaaaag gccgctcatc cttaagtgat gacaaaaaat catcacaatt
     3841 accgatgact gtctctttac tatcttttaa tcagaaaaag gggttatgac aagctttgtc
     3901 aacccctttt ttt
//
LOCUS       DAZKKW010000149         3797 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.151, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000149 DAZKKW010000000
VERSION     DAZKKW010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3797
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.151"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..391
                     /locus_tag="ACG8PO_002727"
     CDS             <1..391
                     /locus_tag="ACG8PO_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YopX family protein"
                     /protein_id="HFQ0207984.1"
                     /translation="RKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSYHVRNIDLMQS
                     TGMKDKNGVDIFEGDIVLVSVRNGFDYLDNKVCIVKNSIDYSGLVCATVDEDLEYQIF
                     NTELFEEYTYEVTGNIYENSELLEEQR"
     gene            388..945
                     /locus_tag="ACG8PO_002728"
                     /pseudo
     CDS             388..945
                     /locus_tag="ACG8PO_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286545.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
     gene            942..1238
                     /locus_tag="ACG8PO_002729"
     CDS             942..1238
                     /locus_tag="ACG8PO_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0207985.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKSKFKVGDMVRCEA
                     EEFVYPFRGYVKKILSNSAIIRIENTMKCDRSTAKHKHYLAVARLVDMEVIKSK"
     gene            1315..1728
                     /locus_tag="ACG8PO_002730"
     CDS             1315..1728
                     /locus_tag="ACG8PO_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family phage packaging/lysis
                     transcriptional regulator"
                     /protein_id="HFQ0207986.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIETERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGKIIAYK"
     gene            2037..2189
                     /locus_tag="ACG8PO_002731"
     CDS             2037..2189
                     /locus_tag="ACG8PO_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0207987.1"
                     /translation="MKLSVEGTPEEIQELLQAIGSSKEQKVSVSNSDIDGICNCLNRE
                     KISNPY"
     gene            complement(2186..2461)
                     /locus_tag="ACG8PO_002732"
     CDS             complement(2186..2461)
                     /locus_tag="ACG8PO_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0207988.1"
                     /translation="MYKPYMISYDLNNPGQKYDKVFEIIKEFGAYIKLQKSFWLVKTN
                     LNPNQMCEKLNTVLDNNDSLFICELQKNYQGRATEENWKFINEHIFS"
     gene            2957..3121
                     /locus_tag="ACG8PO_002733"
     CDS             2957..3121
                     /locus_tag="ACG8PO_002733"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0207989.1"
                     /translation="MPATLNAYDGYDYRKKIVKKLYGATYGNVVSVLSVHHQASVTVA
                     EVEDAAVNGE"
     gene            3317..3484
                     /locus_tag="ACG8PO_002734"
     CDS             3317..3484
                     /locus_tag="ACG8PO_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFQ0207990.1"
                     /translation="MYRPQYLEQKYEVITVHKGNGEKVYEYRRPIKSDTYKRKENNEV
                     IPLYGKRIAKH"
     gene            3510..>3797
                     /locus_tag="ACG8PO_002735"
     CDS             3510..>3797
                     /locus_tag="ACG8PO_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFQ0207991.1"
                     /translation="MTEEFYRWLLQLTREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALD"
BASE COUNT         1310 a          591 c          810 g         1086 t
ORIGIN      
        1 acgaaagaac gtaatgagag atgtagccgt cttgcatttt actaaaaacg gcaaaacaaa
       61 ctttattgaa tattggataa atcctaccga attgaaatca tatcacgtgc gaaacatcga
      121 cctcatgcaa tccacaggaa tgaaagataa gaatggtgtg gatatatttg aaggggatat
      181 agtattagtc agcgtgcgaa atggcttcga ttacttagat aataaagtct gtattgtcaa
      241 aaattcaata gattattccg gattagtttg tgccactgtt gatgaagact tagagtatca
      301 aatttttaac acagagctgt ttgaagaata cacgtacgaa gtcaccggaa atatatacga
      361 gaatagcgag ttattggagg aacagcgatg aataaacagg aagcaatcaa aaagttagaa
      421 agtattaaag cgataggaaa tgatgcaata gctgcttgct ataacgagag tataaattca
      481 ggtattacgt taatgaaaaa aatagacgaa ccgcagaaac cagttattcc acaactcgtg
      541 gccggttggc ttgagaaatc tacggaccct tttacaaaag ctgaaaaaat agcgtattta
      601 atcaaatcta aagatggtga ttcatattat ttctgtgatt ggtttgtacg agatggcata
      661 gtgacgcaag agcaaggaga agaattactc gcttgggcaa caagacaatc atatgaaaca
      721 ctattgagcc tatacaacgg ctaagaggtt gagaaagagc cgttatgggc aataaagaat
      781 gccgatggaa actatcttac taaatgtgct ttatggggaa aagatggagt aaattatagt
      841 tttgaatgca atccatctca tcgattgctt ttcactgata aagcaacagc ggatgctgca
      901 gcattgttgg tgaatggaac agtggaagag gtggtagaaa gatgaaacta aaagacggat
      961 tttacgctag cagtcacggt atcggcggtt taatgctaga tatgccgaca aagaacccta
     1021 aaacacgcaa gaaatcaaaa ttcaaagtcg gtgacatggt ccgctgtgaa gcagaagagt
     1081 tcgtttatcc gttcagagga tacgttaaaa agatactgtc aaactcagca atcattcgta
     1141 ttgaaaatac aatgaagtgt gatcgttcga ctgcaaaaca caagcattat ttagctgtag
     1201 cgagattagt tgacatggaa gtaatcaaga gcaaataaaa aaagccggat cgctccgact
     1261 gattcaataa atccaacaca tttattatat cacataaagg agcggtttga cttgatgcaa
     1321 ttgttacgag aggtagattt caaacagaca agatgtaatg cgagagatgt gctgaagaac
     1381 tttcggcgtt tggagcggat ggcaggtcgc tctttgatag atattaagtc gccgattata
     1441 acggatatgc cgaaggcacc gaagcatggc aataaggcag aagacgcgat tattcagatg
     1501 atggatatag aaacggagag agatgcgatt ctagcggctt tgatggctct tagtctgatt
     1561 agtcgtcaga tactctacta cagcttctgt gtgccagata gcttctcaaa ctacagaatt
     1621 agccgtgaag tgggttattc agaaagaagt atacaacgga tgaagtcgga agctctaata
     1681 gagtttgcag aagcatataa acacggaaaa ataattgctt ataaataatt tggcggtttt
     1741 ttggcggaat gatggcggtt tttagacatt taccagtgtt attatggtag tgtcgaaaga
     1801 ttaggaaaca ggatcgacaa aataaacgaa gggaggcaat ctccctcatc gttttaaact
     1861 tcttcttaga tagacagcaa ctaattatga agaaaaggat gtgaattcaa ctcctcctaa
     1921 attcttcatc ggttgctgtt tattttgatt agacagcagc actcacaaaa ctaaacatgt
     1981 tcttgtcgtc tttcaactgc tgctgtctat taatttatgt attggaggga agaaaaatga
     2041 aattatcagt agaaggcact ccagaagaaa tacaagaatt gctccaagct attggtagta
     2101 gcaaggagca aaaggtaagt gtttctaatt cagatattga cggtatttgc aattgtttaa
     2161 acagagaaaa aatatctaat ccttactaag agaaaatatg ttcgttaatg aatttccaat
     2221 tttcctctgt agctctacct tgataatttt tttgcagttc acagatgaat aaggaatcgt
     2281 tattatcaag tactgtattt aatttttcac acatttgatt tggattcaaa ttagttttaa
     2341 ctaaccaaaa agatttttgt agctttatat atgccccaaa ttcttttatt atttcaaata
     2401 ctttatcata cttttgccct gggttattaa gatcataact aatcatgtag ggtttataca
     2461 tgttgtcttg cacatccttg ttttatattt cagcggacca ctcgctgata aataaaatta
     2521 tacgcttagt atttattttc acaatattaa tttatcgtaa taacttttga gtaaatagaa
     2581 caaaaaacct gcactagttt ccgctagtac aggcagtgac tatatcggtt gataatctag
     2641 catatattaa aaataattgc aagaaaagaa atttatagtg gtttatacca aattatcaaa
     2701 gcaatgttat tttgttgctg tctattgttt tttaattatt cacacgataa ctaaaggtgg
     2761 gtgaagagaa atgattccat taataatttc aatttttgcg ctctgtctta atgtctatat
     2821 gattggattt aaaaatgggc aaaataaaaa atagtagcag ccaagaataa ttttatagtg
     2881 tcactgtggc ggaaagggta gacgctaagc atgtgtgcta ggtcaatgct tcggcaacca
     2941 tgcaaggttc gattccttgc cagcgacatt aaatgcctat gacggttacg actaccgaaa
     3001 aaagatcgtt aagaagctat acggtgctac gtacggcaat gtagtaagtg tgctatctgt
     3061 acaccaccaa gcttcggtca ctgtggcgga agtagaagac gcagcggtaa atggcgagta
     3121 gcctcgtgag agcctggtaa gttctcgtga gtggtgcaat cccactccag cgactttaag
     3181 caaccgaggc atcggcggtt taaaaatata ggggtgcgca atttcgtacg cgttttgtgc
     3241 atcgtgcaag ttactattac atattagatc actctttgag tggtcttttt atttttgcac
     3301 aaaggaggaa acaacaatgt atagaccaca atacttagaa cagaagtatg aagtaatcac
     3361 tgttcataaa ggtaatggcg aaaaagtata tgagtataga agaccaataa agagcgatac
     3421 atataaacga aaggaaaaca atgaagttat tccattgtat ggcaaaagaa tagctaagca
     3481 ttaaataaga ttgcgaaagg agacggaaca tgaccgagga attctataga tggctattac
     3541 agttgacaag agaagatcgt ttggttaagt tctatcagtc tcctaaatgg cgcaggctta
     3601 gagagaaagc gatgaaacga gatcactatg aatgccaaga gtgtagaaga ctaggtaagt
     3661 atcatagagt agagaacgtt catcatataa aggaagtcaa ggatagacct gacttagctt
     3721 tagatttaga taatcttatt tgtttatgtg ttgaacatca taatgaagtt catggcagat
     3781 atcttacagc attagat
//
LOCUS       DAZKKW010000150         3784 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.152, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000150 DAZKKW010000000
VERSION     DAZKKW010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3784
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.152"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(159..1253)
                     /locus_tag="ACG8PO_002736"
     CDS             complement(159..1253)
                     /locus_tag="ACG8PO_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HFQ0207992.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            1442..2203
                     /locus_tag="ACG8PO_002737"
     CDS             1442..2203
                     /locus_tag="ACG8PO_002737"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFQ0207993.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            complement(2318..2758)
                     /gene="fabZ"
                     /locus_tag="ACG8PO_002738"
     CDS             complement(2318..2758)
                     /gene="fabZ"
                     /locus_tag="ACG8PO_002738"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFQ0207994.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            2978..3733
                     /gene="fabI"
                     /locus_tag="ACG8PO_002739"
     CDS             2978..3733
                     /gene="fabI"
                     /locus_tag="ACG8PO_002739"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HFQ0207995.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
BASE COUNT         1220 a          734 c          663 g         1167 t
ORIGIN      
        1 attgaattac agaacaccaa tcgaaatatt tttgagctat gtacaagaag cattttattc
       61 tagcttaatt tgacaaatca taacctataa aaaaacaaaa taaaaaagca gaaataactg
      121 gctatttcta gcggattatt tctgcaataa ctaattatct attttttatt ttcaattaat
      181 ttaaagcttg accatggttt caaaaagtca aggaggaata attcgtcatc ggagattctt
      241 cctacaacat tgacacgtcc gtcgtttttc atatccttca aggcgatttg tgtttctcct
      301 ttgtattgac cgtacccttc attgtcaatc agtacatccc catgatgcat atctcgcgta
      361 ttatgaggcg ggaaatcttt atctttgtag tatacacgag tcatcgttga acgaaggata
      421 tattctgagc gatcccctcg ataactatga aggttatcaa acaagcagat tcgttcgttt
      481 tctgtaatac cttcttccgt atctactttc aatgttggat aatccgcgtt aaatgcttct
      541 gccatttctt tcaattcttc ttcacttgcg tacgcatttc caatagaaat atcatcaatc
      601 aatcctgtaa ggattaaatg cttcacttgt gtcgcaattt caagatctcg gtggtcttcc
      661 agcgtgcaca agccatcttg cgtaggccaa gggccaaact cagcagattg cgaattgacg
      721 aatgccatcg tattcaaatt atattttctg aatttttctg aacaaaaaac aaagtgttcg
      781 tatcctaaac cagagtatcg atgaggataa aaattatgag aacctaataa attatccgtg
      841 tttggcgaat aactcatgat gttgtcaacg taattcgtgc ccgaactcat attgatctcg
      901 attttgatac caaaaggatt gcgcgtcatt tttgcttctt ctgctcctgt gaatccgata
      961 tctaagcgga ccccgtaggc acccatctta tggaaaaaag ataagtcatc gtaagaaatc
     1021 tccaactgtt caaataacgc tggattgata tcaaccatta cttccatgcc taggctattc
     1081 gcgtaatcaa ctacttcttt aaattctgaa agaacttttt ctttatcatc attgatctgc
     1141 agcaaactgg taaaaacccg tttataccca tacttatgag ctaaatccaa gtacgctttg
     1201 tccttttcaa atgttgaccg ttctggataa atcgagattc ctaattttcc catattacca
     1261 tttcctcctg catcttttcg tttttgtatc cacttacatt atagcaaaca caaagtaaat
     1321 ggtctacacc ttttcttttt cttttaaaat aaatgataaa ttccaataat ttaagtaggt
     1381 attttccctc tattttgtta tcattaaaga taacaaaata gtaaattgga aaggaacagt
     1441 tatgaagaaa aataaagtta ttttaagtat tagtttagca gcagcattgc tgtttgctgc
     1501 tggttgttct gcaaacaaca cgacttcttc tagttcgtct acagaaagtt caaaaagtac
     1561 atctactgcg gaaagcacga ctgccagtag cgtcgatttg aacgcattgg aattaccaca
     1621 attgacgaaa gatgtcgccg aaaatgaaga tctggtcgaa atggtgacga ataaaggcac
     1681 aatcaaaatc aaattatttc ccgaacacgc accaaaagcg gttgaaaact ttatgacaca
     1741 tgcaaaagac ggttactaca acggactgac tttccatcgc gtcatcaaag atttcatgat
     1801 ccaaggtggc gatcctaaag gcgatggcac tggaggggaa agcatttggg gagaaggatt
     1861 tgaaacagag atcaacaacc acctttataa tataagaggt gcgttatcta tggctcgctc
     1921 acaagatcct aacagtaacg gcagccagtt ctttatcgtt caaaatacag atgatatgca
     1981 tgatggtctt ttaaaagacg actatcctca agcaatcatc gacgcttata aaaacggcgg
     2041 ttatccttca ctagatggcc attatactgt cttcggccaa gtgattgaag gaatggatgt
     2101 cgtggatgct attgcaaata tcgaaacaga tagttcagac aaaccaaaag aagaagtaaa
     2161 aatcgataaa atcaatatac tgcaagaagc aaaacaaaaa taaaaaaata acgtctgaat
     2221 cctgcataga tgacaacgta tgctaggatt caggcgtctt ctttttacat aaaaagcaag
     2281 taagcttcct tttgttttgc tatatcaggt tttatagtca agcattttgg ttgacgatga
     2341 acgtgaactc gcactcacag acttttttat catctacata tgctgcagat aacgctgtac
     2401 cgactgatcc cttcattttg gtgatttcaa aatgacattt cagcacatct cctggaacaa
     2461 ctttttgacg gaatttggcc ttattgatcc caccgatata agctcttttt cctagaaact
     2521 cttctgattt taggataagt atcgaacctg cttgtgccag tgtttctaga atcaacgcac
     2581 ccggcatcac tggattgtca ggaaagtgac cttggaaaaa atcttcattg attgttacat
     2641 tttttgtcgc aacgatcttt tttcctggtt ctaaagtatc tacataatca atataacaaa
     2701 taggataacg attaggaatc aattccatta cttcagtagc agataatacg taagacataa
     2761 ttaaactcct tctttcaggc aattgccatt ttcattactt tgactatcaa agtatcatat
     2821 tcctaaatat ttagcaatct ttttttaatt taagaaaaaa tagtttgaca atcaaaacat
     2881 tttgactaat aattaagaaa tcaagaagtt attttagcta ttattctcta tttttttacg
     2941 actatcaaat ttttatcaga aacaaaggag agtcattatg tcatttctag aaggaaaaaa
     3001 aatcgtcatt atgggcgtcg ctaacaaaaa aagcattgct tggggttgtg ctgaagcaat
     3061 gttgaaacaa ggggcagaaa ttatctatac ttaccaaaat gaacgaatga aaaaatcatt
     3121 agtcaaattg gtcggcgaag aagcattcac tgtagaatgt gatgtttcta gcgacgaaag
     3181 catcgaaaaa gcaatgaatg ccatcggtga atatgctggg gagatccacg gccttgtcca
     3241 tgcagttgct tacgccaaca aagaagaatt gtccggaaat gtgtctgata tttccagaga
     3301 cggctatcaa ttagctcaag atatcagcag ctactcttta attgctgtat caaaatacgc
     3361 acaaccgttt cttgcaccaa acagcgggat cgttagcatg acttatctag gttcagaacg
     3421 cgcaatcccg aattataaca tgatgggtat tgcgaaagct tctcttgaag ctgctatccg
     3481 ttatttggca gctgagttct cgcctaaagg aattcgggtc aatggaatct cagctggggc
     3541 aatcaaaaca ttagctgtca caggtgttaa agactatcaa aaactgatcc agttatctga
     3601 atctcgtacg cctgatcagc aaggtgtgac gatcgaagaa gtcggtaatg cttgtgcttt
     3661 cttaatcagc ccactttcca gcggaatcat cggtgatatt atctacgttg acaaaggtgt
     3721 ccatctttca taaacttttg ttgcattctt taaagtaaaa catataaaaa tcaggaaaaa
     3781 actt
//
LOCUS       DAZKKW010000151         3765 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.153, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000151 DAZKKW010000000
VERSION     DAZKKW010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3765
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.153"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            10..615
                     /locus_tag="ACG8PO_002740"
     CDS             10..615
                     /locus_tag="ACG8PO_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFQ0207996.1"
                     /translation="MAKTTTVTTFDNISIKRISFNFKNATNAISTDCNGQLDGETEMQ
                     RIVKKCGSTEVKSKSKPINMTVTITAHVPMEVYRRFNGLKQDERIKSGIYSYGPDSVG
                     EDFSLAAEIVDDFEEKNKLIGMLACTSNTGLTFSIENGADEVAALELETKVMQDEFGK
                     FYHEAIVAELEEDLTDQWMTNLSADVIKKTSVVTTTATQSQ"
     gene            635..997
                     /locus_tag="ACG8PO_002741"
     CDS             635..997
                     /locus_tag="ACG8PO_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0207997.1"
                     /translation="MNEDYSKIELNDGTILNLEPKLNIKKLLMINRDFNTDEFAKMTV
                     GKGSMDISVIQGAKAVYIAYRQANMTDYISFDEFIDKWDFDMATASYIYQLMMFKQAR
                     DAYQKEFEKANKEKKLQK"
     gene            1000..1182
                     /locus_tag="ACG8PO_002742"
     CDS             1000..1182
                     /locus_tag="ACG8PO_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0207998.1"
                     /translation="MPKLLVETWVDVYSMLTDVFSMPSDLVLSDICLDDILQMAYNKS
                     AYEGWKNYAINQSQKN"
     gene            1199..>3765
                     /locus_tag="ACG8PO_002743"
     CDS             1199..>3765
                     /locus_tag="ACG8PO_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFQ0207999.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVQKLERELIN
                     QQTAQQRLSNEIDKTSNALAQAKGEIQTYESTMQQLDSEQKNVQASASLIESEYKKWQ
                     ATAGQSASESEKLAKAQEYVSQQSENAEKTIDILRRQLEATQSEFGATSTEAMQMEAK
                     LNDAEREFEELGQAAKNVDTTNLDDIGSKIDMNNLMEASDVLSDIGDKLTELGKQAVD
                     SANSVGSSQSKIQANFGLTKQEAEELTNVARDIYYKGFGESLDQSTDALILVKRNLGD
                     LNNQDLQNITEQAMVLENTMGADMDETLRGVNGLMVNFGLSAQDAMDLMVSGTQNGLD
                     KTHELGDNMAEYSQLWSQMGYSADETFGMLQNGLDAGAYNLDKVNDLVKEMGISLTDG
                     RFEQNMDMFSESTRKAFEEWKNGGGTQKDVINSMIQDFSNMDGQYDQLNKASTIWSAL
                     GEDNAMKVVQSLTDVNHTFDDVSGSAQKMNEDSTTPLQELNGKIAELKDSLAPIGNTI
                     IDALEPVIDFLGKMADAFNNLPQPVQDYAVAIGGLTAAFTLLMPIIVGFMALGGPTTL
                     IIGAVITVIAGVIAIIKNWGAITDWFKGIWSKFTDWLGGTWESIKEGASSVWDGVKET
                     WSGFVDWVQDIWQGVSDWFGELWSGLVEGASNIWQGVQETWQAFVDWVSNIWNGVKEV
                     WSIIWADIVGIVQIPWTLITSLIQAGINIIVGIFDVAGQLLGAAWQAVWTPISDFLKN
                     TWDTMTQWVSIAWNGIVTTFHTIFDPVVAWWNGIWTAISTTASNIWNSISATASSIW"
BASE COUNT         1355 a          561 c          818 g         1031 t
ORIGIN      
        1 ggaacaacaa tggctaaaac aacaacagta acaacttttg acaatataag tattaaaaga
       61 atttctttta attttaagaa tgcaacaaat gcaatttcaa ctgattgtaa tggacaatta
      121 gatggcgaaa cagaaatgca gagaattgta aaaaaatgcg gttcaacaga agtaaaatcg
      181 aaatctaaac caatcaatat gacggtaaca attactgcac atgtaccgat ggaagtttat
      241 cgacgcttca atgggttgaa acaagatgaa cgtattaaat cgggaattta ctcttatggt
      301 cctgattccg taggggaaga tttctctctt gctgcagaga tcgtggatga tttcgaagaa
      361 aaaaataagc tgattggtat gttagcatgc acttcgaata caggattaac attctctatt
      421 gaaaatggtg cggatgaagt agctgcgtta gaactagaaa caaaagttat gcaagatgaa
      481 tttggtaaat tttatcatga agcaattgtt gcagaacttg aagaagactt aacagatcaa
      541 tggatgacga atctatctgc tgatgtgatt aaaaaaactt cagttgtgac aactacggcc
      601 actcaatcac agtaaaaaaa acggaggtag cgaaatgaac gaagattact caaaaattga
      661 actaaacgat ggaacaattt tgaatttaga acctaaactg aatatcaaga aattattgat
      721 gatcaataga gattttaaca cagacgagtt tgcaaaaatg actgtgggaa aaggatccat
      781 ggatatttct gttattcaag gtgcaaaggc tgtgtatatt gcttaccgcc aagcgaacat
      841 gactgattat atttcattcg atgaatttat cgataaatgg gattttgata tggctactgc
      901 cagctatatt tatcaattga tgatgttcaa acaagcacgc gatgcttatc aaaaagaatt
      961 tgaaaaagca aataaggaaa aaaagcttca aaagtaaaaa tgccaaagct cttagttgaa
     1021 acgtgggtcg atgtctattc gatgttgacc gacgtttttt ctatgccttc agatttggtt
     1081 ttaagcgata tctgtttaga tgacattttg caaatggctt acaacaagag tgcttatgaa
     1141 ggatggaaaa actatgcaat aaaccaatcc cagaaaaact aaagaaagga ggtaaaaaat
     1201 ggctaaaaag agaacagaag cagaagtaac attcatagct aacgatgacg gattgaaatc
     1261 tacgttaaaa gaaatcagtg ctgaattaac taaaaataga gcagaattaa aactagaaca
     1321 agctcaatta caacagactg gttctgaatc agacaagtta ggaagtaaat tatcttcttt
     1381 agaaaagcag tatgaattac aaagtcaaaa agttgaagta actagccaac gtttagccaa
     1441 tgccaaaaaa tattatggag aaaattccac cgaagttcag aaacttgaga gagaactgat
     1501 taaccaacaa acagcgcaac aacgtttgtc aaacgaaatt gataaaacga gtaatgcact
     1561 agctcaagca aaaggcgaaa tacagacgta cgagtctaca atgcaacagt tggatagtga
     1621 acaaaaaaat gttcaagcta gtgcttctct gattgaatcc gaatacaaaa aatggcaagc
     1681 aactgctggt caatcagctt ctgaatccga gaaattagcg aaagcccaag aatatgtttc
     1741 tcaacaatct gaaaatgcag agaaaacgat agatatcctg agacgacagt tagaagctac
     1801 acagtctgag tttggcgcta catccacaga agcaatgcag atggaggcga agcttaatga
     1861 tgctgaacgt gaatttgaag agttaggaca agctgctaaa aatgtagata caactaactt
     1921 ggacgatatc ggaagcaaaa tagatatgaa taatctaatg gaagcttctg acgttttaag
     1981 cgacattggc gataagctta cagaattagg gaaacaagca gtggactctg ctaatagtgt
     2041 aggtagttcc cagagtaaaa tacaagctaa ttttggtttg actaaacaag aggctgaaga
     2101 attaacgaat gtagccagag acatttatta taaaggtttt ggagaatcgt tagatcagtc
     2161 cacagatgca ttgattttgg taaagcgtaa tttaggcgat ttaaataatc aagatttaca
     2221 aaatatcacg gaacaagcta tggtcctaga aaacaccatg ggcgctgata tggatgaaac
     2281 gttacgtggt gtaaatggct taatggtcaa tttcggcttg agcgctcaag atgcaatgga
     2341 tttaatggtt tcgggtactc aaaacggttt agataaaacg cacgaattag gcgacaatat
     2401 ggcagaatat agccaattat ggagtcaaat gggatattca gctgatgaaa cgttcggaat
     2461 gcttcaaaat ggtttagatg cgggtgctta taaccttgat aaagtcaatg acttagttaa
     2521 ggaaatggga atatcgttaa cagatggtcg atttgagcaa aacatggata tgtttagtga
     2581 aagtactaga aaagcttttg aagagtggaa aaatggcgga ggaacacaaa aagacgttat
     2641 taattccatg attcaagatt ttagcaatat ggatggtcaa tacgaccaat taaataaagc
     2701 ttctacaatt tggtctgcac ttggcgaaga taacgcgatg aaagttgtcc aatctttgac
     2761 tgatgttaac catacatttg atgatgttag tggatctgca caaaaaatga atgaagattc
     2821 tactactccg ttacaagaat taaacggaaa aatagctgaa ttaaaggatt cattagctcc
     2881 tataggcaac acaatcatag atgcactcga accagtaatt gattttctag gaaagatggc
     2941 tgatgcgttt aataatcttc cacaaccagt acaggattat gccgtagcga ttggcggatt
     3001 gactgctgca tttactttat taatgccaat aatagttggc ttcatggctc taggtggtcc
     3061 tactacatta ataataggag cagttattac tgttattgct ggagttatag caattataaa
     3121 aaactggggc gcaattactg actggtttaa gggaatatgg agtaaattca ctgattggtt
     3181 gggtggtact tgggaaagta taaaagaagg tgcctcatca gtttgggatg gagttaaaga
     3241 aacctggtct ggatttgtag attgggttca agatatttgg caaggagttt ctgattggtt
     3301 tggagagcta tggagcggat tagttgaagg agcttccaac atctggcaag gagtccaaga
     3361 gacttggcaa gcattcgttg attgggtttc aaatatttgg aacggagtca aagaagtatg
     3421 gtcgattatt tgggcagaca ttgtaggaat tgttcaaata ccatggacct taataacgtc
     3481 attgattcaa gccggtatta atattatcgt gggtattttt gatgtagctg gacagttatt
     3541 aggcgcagct tggcaagctg tttggacacc tatttctgat ttccttaaaa acacttggga
     3601 tactatgaca caatgggtaa gcatcgcttg gaatggaatt gtaactacat tccatactat
     3661 atttgatcca gtagtggcat ggtggaatgg tatatggaca gctattagta ctacggcttc
     3721 aaatatttgg aattcaatta gtgcaacagc ttctagtatt tggaa
//
LOCUS       DAZKKW010000152         3739 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.154, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000152 DAZKKW010000000
VERSION     DAZKKW010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3739
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.154"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            280..879
                     /locus_tag="ACG8PO_002744"
     CDS             280..879
                     /locus_tag="ACG8PO_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0208000.1"
                     /translation="MKSVEPIRDKKKIDAMKAILASGKYGQRNLVLFSIGINTAYRIS
                     DLRQLKLRDVLEISRGRVIVKERLAMKEQKTAKHNSVFISNKLRKVILDYVQSEFPEQ
                     LQAQDFSKYLFPSRKGADTPLTRQSLWRIIHEAGTAVGLKEIGPHSMRKTFGYFLYKQ
                     GTKTEIIQSLLNHSSQRETLRYIGITQEDKDTAVKSLDL"
     gene            928..1644
                     /locus_tag="ACG8PO_002745"
     CDS             928..1644
                     /locus_tag="ACG8PO_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HFQ0208001.1"
                     /translation="MNEEKQPNFPDKYHLSRKESVYLLKKNIVELVYNAGKFEGLNTT
                     LLQTEGIIKYNRANNVAVDDVLTVVNLKRGFEMLLNDVQEPLLETSKRINRIVAAEDA
                     LFPGEIRTGGVELSTIQGRYVPPMLTEDEVNNQYGEIMNQEISDTEKALRLFLFISKN
                     QIFWNGNVGTALLIANKIMFSNGLGLLSIPENVFMKFNELLSKYCNSNLLVNESNIIS
                     FLYEQCIFGINYLKIIQHTI"
     gene            1658..2437
                     /locus_tag="ACG8PO_002746"
     CDS             1658..2437
                     /locus_tag="ACG8PO_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002396205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection family protein"
                     /protein_id="HFQ0208002.1"
                     /translation="MMTIDEAKQLATYFSVGSAGYILDFTNVTWNIFTKKIVGIEIQN
                     HYGGSKGVSFQKFLLDDQFDDSLKNRLIQSLKGIMDRDKKYESTEMNPIVQLLFEEVF
                     KEDTSTALRKMKFSSEIDLLYIRQLPIRIQTDIKNKDFDSVLTKANTLIDEVLKFIIE
                     NTEGPSVNINLFKSKELRKEVFGRLNIKTEKDMDNRIKALVGALNTLSDKILEMRNSQ
                     GDAHAHGSNRIVIKEEEAILVANSSMVLCEYVFTKYKGLNR"
     gene            2520..2876
                     /locus_tag="ACG8PO_002747"
     CDS             2520..2876
                     /locus_tag="ACG8PO_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFQ0208003.1"
                     /translation="MEKISFETFKRPNGHDEFLEWLETLPKKDSAKLLRTIEETEKNG
                     MLVAQRLKWVKKLDTNLYELRSKVGSNIQRAIYFHVDNGRYVITHGFTKKTEKTPITE
                     LKHAKELREEWFTNDD"
     gene            2866..3132
                     /locus_tag="ACG8PO_002748"
     CDS             2866..3132
                     /locus_tag="ACG8PO_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0208004.1"
                     /translation="MMIKKLIEEKKKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
     gene            3291..3569
                     /locus_tag="ACG8PO_002749"
     CDS             3291..3569
                     /locus_tag="ACG8PO_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0208005.1"
                     /translation="MKTINLIKVFIASPGDIAAKRNEVEETIYRWNIENTDNTKVILM
                     PIRWETNAIPTYVNNSSGQSVINKQIVENRVLLQSFKNKISFCIISSF"
     gene            complement(3566..>3739)
                     /locus_tag="ACG8PO_002750"
     CDS             complement(3566..>3739)
                     /locus_tag="ACG8PO_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010868911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HFQ0208006.1"
                     /translation="HRSIKRRHKLYQSLRTASTTIKGMETIRGIYKKSRKEGTLFGFS
                     VCTEIRILLGIPA"
BASE COUNT         1380 a          537 c          732 g         1090 t
ORIGIN      
        1 gactgaaaat tttgaatttt cagaatagaa taacttaaaa gtactgacaa attttttaaa
       61 ggggcaatat taatgttctg agataatcaa atcttaccga aaatatcaaa agtccaatgt
      121 gacacaattg gattttgtca cattcgttaa aatcgctcca tccttagagg ctctaatagg
      181 aaatcacttt tcttttagcg ccttttttct atagcttgtt taaatgtgac ttattatctg
      241 ttataatcgt attaagtaac atttgagagg tgtctaaaaa tgaaaagcgt ggaaccgatt
      301 cgcgacaaaa aaaagatcga tgccatgaag gccattttgg cttccggaaa atatgggcaa
      361 cgaaatttgg tgcttttttc gatcgggatc aatacagcct atcgaatctc cgatttaaga
      421 cagttaaaat taagggatgt gctagaaatt tcccgtgggc gggtgatcgt caaagaacgt
      481 ctggccatga aggaacaaaa aacggccaaa cacaattcgg tcttcatttc gaacaaattg
      541 cgaaaagtga ttcttgatta tgttcaatcc gaatttcctg agcagttgca agcacaagac
      601 ttcagcaagt atttgtttcc aagtcgaaaa ggagcagata cgcctttgac ccgacaaagc
      661 ctttggcgga tcattcatga agcaggaact gcagttgggt taaaagaaat tggtccccat
      721 tcgatgcgta agacctttgg ctatttttta tacaagcaag gaaccaaaac agagatcatt
      781 cagtcgctgc taaaccattc ttcacaacgg gagaccttac gctatattgg aatcacccag
      841 gaagacaaag atactgcagt aaagagtttg gatttatgac agaataatgc cctatataat
      901 agaaggaaaa attcaagagg tgagaaaatg aacgaagaaa agcagcctaa ctttcctgat
      961 aaatatcact tatctcgcaa agagtctgtg tatttattga agaaaaacat tgtagaactg
     1021 gtgtataatg cggggaaatt tgaagggtta aatacaacgt tgttacaaac agaagggatt
     1081 attaagtata atcgggccaa caacgtggct gtagatgatg tcctgaccgt cgttaactta
     1141 aaaagaggct ttgaaatgct tttaaatgac gtacaggagc ccctgttaga gacaagtaag
     1201 cggattaatc ggatcgttgc agcagaggat gcgctttttc ctggtgaaat acgaaccggt
     1261 ggggtagaat tatcgacgat tcaaggaaga tatgtgccac caatgttaac tgaagacgag
     1321 gtaaacaatc aatatggtga gataatgaat caagagatat cagatacgga aaaagccctg
     1381 cgactgtttc tattcatttc gaaaaatcaa atattctgga atggtaatgt gggtacagcc
     1441 ttgttgattg cgaataagat tatgtttagc aacggtttag gactattatc aatacccgaa
     1501 aatgtattta tgaaatttaa cgaactttta tcaaagtatt gcaacagtaa tctattagtc
     1561 aatgagtcaa atattatatc atttttatat gaacagtgta tctttggtat taattatttg
     1621 aaaataatcc aacatacaat ttaagaggta taagtaaatg atgactatag acgaagcaaa
     1681 acaattagca acttattttt cagttggttc agcgggatat attttagact ttactaatgt
     1741 aacgtggaac atatttacaa agaaaatcgt agggattgaa atacaaaatc attatggggg
     1801 ctcaaaggga gtatcctttc aaaagtttct gcttgacgat caatttgatg attcgttaaa
     1861 gaatcggtta atccaatctt taaaaggtat tatggatcga gataaaaaat atgaatctac
     1921 tgaaatgaat cccatagttc agttgctttt tgaggaagta tttaaagaag atacttcaac
     1981 tgcattaaga aaaatgaaat tttcaagcga gattgattta ctttatattc ggcagctacc
     2041 cattaggatt caaacagata ttaaaaacaa ggattttgac tctgttttga caaaagcaaa
     2101 tactttaata gatgaagttc taaaatttat tattgaaaat accgaaggac cgagtgttaa
     2161 tattaattta tttaagtcaa aagaattaag aaaagaagta tttggccgcc tcaatattaa
     2221 aactgaaaaa gatatggata atagaatcaa agcccttgtt ggagcactca atacattatc
     2281 agataaaata ctcgaaatga gaaatagtca aggagatgct catgcacatg gctcaaatag
     2341 aatagtaatt aaggaagaag aagcaattct tgttgcaaat tcttctatgg ttttgtgcga
     2401 gtatgtgttt actaagtata aaggattaaa tagataataa aatgcatatt ttatagtata
     2461 caattcgaat ttttatgtta tatactataa tataaaatca atatatgata taattgggta
     2521 tggaaaaaat atcttttgaa acatttaaaa gacccaatgg tcatgatgaa tttttagaat
     2581 ggttggaaac attaccaaaa aaagatagtg cgaagctttt acgtacaata gaagaaacag
     2641 aaaagaatgg gatgctagta gctcaacggt tgaaatgggt gaaaaaatta gacactaatt
     2701 tgtacgaatt aagaagtaag gtaggatcaa acatccaaag agccatctat ttccatgtgg
     2761 acaatggaag gtatgtcatt actcacggat ttactaaaaa aacggagaaa acaccaataa
     2821 ctgaactcaa gcatgcaaaa gaacttagag aggagtggtt cacaaatgat gattaaaaaa
     2881 ttgattgaag aaaagaaaaa agataaagat tttgcagaag catatgaccg tgaaggaaga
     2941 aaactcgaaa ctgcggttgc tctttatcat gctcgggagg aagcagggct aactcaagcg
     3001 gagcttgcac aaaaagcaca tacaactcaa tctactattg ctcgaattga gagaggggat
     3061 aacgtatctt tcgataaact tgatgatatc gccagggcat taggtaaaaa gctggtagta
     3121 gaatttgctt aaaagcgagt tgaaaatata tctataatag gctcctaact aatcaattag
     3181 tttagtgggc ctttttttac tagatagata aaggttgtac tatagtgtaa aagacaataa
     3241 attttatact aaaattactt taaagagaac tagagagaaa ggtacaataa atgaaaacca
     3301 taaatcttat aaaagtattt atagcatcac caggtgatat agcagcaaaa aggaatgaag
     3361 tagaagaaac catatataga tggaatatag aaaatacaga taatacaaaa gtgattttga
     3421 tgcctatacg atgggaaact aatgcgattc ctacttatgt aaataatagc tctgggcaaa
     3481 gtgttattaa taaacaaatt gtagaaaata gggttctgct gcaaagtttt aaaaataaaa
     3541 tcagtttttg tattatttct tcattttaag ctggaattcc caataatatc ctgatttcag
     3601 tgcagactga aaagccgaag agagtgcctt cttttcggct tttcttgtag attcctcgaa
     3661 tggtttccat ccctttaatt gtggtggaag ctgtacgtag actttgatac aatttatgtc
     3721 gacgttttat cgatcgatg
//
LOCUS       DAZKKW010000153         3696 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.155, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000153 DAZKKW010000000
VERSION     DAZKKW010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3696
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.155"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(300..2135)
                     /locus_tag="ACG8PO_002751"
     CDS             complement(300..2135)
                     /locus_tag="ACG8PO_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002414956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase family protein"
                     /protein_id="HFQ0208007.1"
                     /translation="MQTFEIKEEFLVDGKPTKLISGAIHYFRMTPAQWEDSLYNLKAL
                     GANTVETYIPWNLHEPVEGVYDFEGMKDIVAFVSLAQELGLMVILRPSVYICAEWEFG
                     GLPAWLLKEHVRLRSTDPRFMAKVRTYFSVLLPKLVPLQVTHGGPVIMMQVENEYGSY
                     GMEKEYLRQTKQVMEEFGIDVPLFTSDGAWEEVLDAGTLIEEDVFVTGNFGSHSKENA
                     AVMKAFMAKHDKKWPIMCMEYWDGWFNRWGEPIIKRDGQDLANEVKDMLALGSLNLYM
                     FHGGTNFGFYNGCSARGVLDLPQVTSYDYDALLTEAGEPTEKYFHVQRAIKEVCPEVW
                     QAEPRRKTFGSLGTFPVQNSVSLLAVKDQMMTAQETMYPITMEEAGSGYGYMLYSVNL
                     KNYHHENKLKVVEASDRLHLFADGNLQTIQYQENLGEEVMIKGTPEKESIEVDVLVEN
                     LGRVNYGFKLNGPTQVKGIRGGIMQDIHFHQGYRQYALTLSADQLKNIDYTAGKNPAQ
                     PSFYQAEFTLTELADTFIDCRGYGKGVVIVNGINLGRYWQRGPIHSLYCPKEFLKKGT
                     NEIVIFETEGIEINELIFCGQPIVKKLLTNDFSEIGSNIHNHIDS"
     gene            complement(2212..3567)
                     /locus_tag="ACG8PO_002752"
     CDS             complement(2212..3567)
                     /locus_tag="ACG8PO_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004188917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFQ0208008.1"
                     /translation="MIDKIMNTIQNKVLPIATKIGNQRFLVALRDSFIGTMPIVMTGS
                     IALLINAFLVDIPDQFNLQKITSFFQWWVDINNLVFKGSISIVALLFIYCLGVNIAKI
                     YKTDTLSSGLVALSSFIISIGNSMTSTYNLSNNNNIDLTTLFTGVEGIKVTGSSLNVT
                     ISGLLPGTQINSNGYFTAIIIGFLSSIIFCKLMNKNWTIKLPDTVPPAIAKPFLSIIP
                     ALVSLYIVAIFTFSFTKITDLLIIDWIYKVLQTPMLGLSQSFLAVVLVTVLTQFFWFF
                     GIHGGNVMAPIMEGVFGVALLANLEAYQNNETIPYVWTSVSYGSFVWYATLGLLIAIF
                     LVSKNSHYKEVAKLGIMPVLFNIGEPVMYGLPTVLNPILFIPFLLCPAVMSSVAYLVT
                     DLGWVSPVTQNVTWVMPPVLYGFFSTGFDWRSIILSIINLFLATLIYLPFVRMANKKI
                     G"
BASE COUNT         1227 a          769 c          553 g         1147 t
ORIGIN      
        1 cctagactct cattttttat agtttcttcg aaaaaatcaa tgtatctgtg atgaatctat
       61 aattatacct tttatcaacg atcaaatgct taaattatct actatcattt aggatagtta
      121 tcgcgaatat aagtcaatct caaaagaaag tgtaaactat tcactgcttg aaccataaat
      181 gaaaagtaaa aatcattcga gacttgttta gataaatatt tgtgatgcgt tagaataagt
      241 attgagattt ggaaaaatcg cgacacatac aatgattggt ttgctgcaat aaatttaaac
      301 tagctgtcta tatggttatg gatatttgaa cctatttcag aaaaatcatt agtcaaaagt
      361 ttcttgacta tcggttgtcc acaaaagatg agctcattaa tttctatccc ttccgtttca
      421 aaaatcacga tctcatttgt gcctttcttc aaaaactctt tcggacaata caaggaatga
      481 atcggtcctc tttgccagta acgacctaag ttgataccgt tgacgatgac cacccctttg
      541 ccatagccgc gacaatcgat aaatgtatcc gcaagttcag tcaacgtgaa ttcagcttga
      601 taaaaagaag gctgggccgg atttttaccg gcagtataat cgatattttt gagttgatct
      661 gctgacaatg tcaatgcata ttgccgatag ccttgatgaa aatggatgtc ttgcatgatt
      721 cccccgcgaa tgcctttcac ttgtgtcggc ccattcaatt taaagccata gttcactcga
      781 cctaagttct ctactaaaac atctacttca attgattctt tctctggcgt acccttgatc
      841 atcacttctt ccccaaggtt ttcttgatat tgaattgtct gcaggttacc gtctgcaaaa
      901 agatgcaatc gatcactggc ctccacaact ttgagcttat tttcgtgatg ataatttttt
      961 aagtttactg aataaagcat atacccataa ccagatcctg cttcttccat cgttattgga
     1021 tacatcgttt cttgggccgt catcatctgg tcttttaccg ccaacagtga gacgctgttt
     1081 tgcacaggga atgttcctaa agaaccaaat gttttcctgc ggggctcggc ttgccacact
     1141 tcaggacata cttctttaat ggctctttgt acatggaagt atttctcagt cggctcgcca
     1201 gcttccgtca ataacgcatc gtagtcatag ctagtcactt gtggcaaatc caacacacca
     1261 cgagcagaac aaccattata aaaaccaaaa ttggttccgc catggaacat atagagattc
     1321 aatgaaccta aggctagcat atccttgact tcattagcta aatcttggcc atcacgcttg
     1381 ataatcggtt ctccccaacg attgaaccaa ccgtcccagt attccatgca cataatcggc
     1441 cactttttgt cgtgttttgc cataaatgcc ttcatcactg cagcgttctc tttggaatga
     1501 ctcccgaaat ttcctgttac gaaaacatcc tcttcaatca gtgtgcccgc atccagcact
     1561 tcttcccaag caccatctga cgtaaataat ggaacatcga tgccaaattc ttccatcacc
     1621 tgtttcgttt gccgcaaata ctctttttcc atgccataag aaccatattc attttcgact
     1681 tgcatcatga tgactggtcc gccatgtgtc acttgcaaag gaactaattt tggtaaaagc
     1741 acactgaaat aagtacggac ttttgccata aatcgtggat cagttgaacg caatcgcacg
     1801 tgctctttca gcaaccaagc aggtagcccg ccgaattccc attccgcaca aatatagaca
     1861 gatggccgca aaatcaccat caagccaagt tcttgcgcca atgacacaaa agcaacgatg
     1921 tctttcattc cctcaaagtc atagacgcct tccactggct catgcagatt ccaaggaata
     1981 taagtctcta cggtattcgc acccagtgct ttcaaattgt ataaactatc ttcccattgt
     2041 gccggtgtca ttcgaaaata atggatcgcg ccactaatca atttagttgg ttttccatct
     2101 accaaaaatt cttcttttat ctcaaacgtt tgcatgtctt ctactcctta atatatcaaa
     2161 aatgaggctt atcatacttc taatacacac taactacgac ttactaaaaa attatccaat
     2221 tttcttattt gccattctaa caaatggcaa ataaattaac gtggctaaaa ataaattaat
     2281 aattgataaa ataattgatc tccaatcaaa tcctgtcgaa aagaatccat ataagaccgg
     2341 tggcattacc caagttacat tttgtgttac tggagaaacc caacctaaat ctgttactaa
     2401 ataagccaca ctagacatta cagcaggaca taacaaaaat ggaataaata aaataggatt
     2461 taatacagta ggtaatccat acattactgg ttcaccaata ttaaacaaaa caggcataat
     2521 tccaagtttt gcaacttctt tataatgact attttttgat accaaaaaaa tcgcaattaa
     2581 taaacctaaa gttgcatacc agacaaacga tccgtatgat acgcttgtcc aaacataagg
     2641 aatagtttca ttgttttgat aagcttctaa attagctaat agagccactc caaatacacc
     2701 ttccataata ggcgccataa catttccacc atgaataccg aaaaaccaga aaaattgtgt
     2761 taagactgtc actaatacca ctgcaagaaa actttgagat aagcctaaca tcggtgtttg
     2821 taatactttg tatatccaat ctataattaa taaatccgta attttagtga atgaaaaagt
     2881 aaaaatagct actatataca atgaaactag agctggaatt attgataaaa atggtttagc
     2941 aatagctgga ggaactgtat caggtaactt aattgtccaa tttttattca tgagtttaca
     3001 aaaaataatt gaagataaaa aacctatgat tattgcagta aaataaccat ttgaattaat
     3061 ttgagtacca ggcaacaatc ctgatatagt cacatttaaa ctgctacctg taacttttat
     3121 tccctcaacc ccagtaaata gtgttgttaa gtcaatatta ttattattcg ataaattgta
     3181 agttgaagtc atagaattac ctatggaaat aataaatgaa gacagtgcga ctagtcctga
     3241 agacaaagta tctgttttat atattttggc aatatttact cctaaacaat aaataaaaag
     3301 caaagctact atcgaaatgc tacctttaaa aaccaagtta ttaatatcca cccaccattg
     3361 gaagaacgac gtaatctttt gtaaattaaa ctgatcagga atatcaacca aaaaagcatt
     3421 gattaacaac gcaattgaac ctgtcattac tataggcatt gtccctataa atgaatctct
     3481 taaagctacc aaaaatcgtt gattcccaat cttagtagct ataggtaaaa ctttattctg
     3541 aattgtattc attattttat caatcatcac attcactcct tcaaaaagaa aataattaga
     3601 aagcgctatc cagttatagt atataaattt ttttgaccat ttgaacatga taatttatac
     3661 ttaaaacatg gtattttctt cataattagt tctata
//
LOCUS       DAZKKW010000154         3679 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.156, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000154 DAZKKW010000000
VERSION     DAZKKW010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3679
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.156"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..2051)
                     /locus_tag="ACG8PO_002753"
     CDS             complement(<1..2051)
                     /locus_tag="ACG8PO_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail spike protein"
                     /protein_id="HFQ0208009.1"
                     /translation="MIDNLLTIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYSINGTNADLIVEGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITATQAWQNAKVLAKPAIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLRHEKSLGTDHKIKAIYTKNLTGLDLEIDAQS
                     VLVGVYPFISSSSEGEDEITLPEEVIFTDYVDDYPAGYVSFVDFKDKATDVATLREAA
                     KDWLKTNIDKQKPQVSGSIELVPLRHQRGYEKFVDLEKVSMGDGVDVYHPQLKVNMSA
                     RIVEYTFNVLTNSYDKLVVGNVKTNFLENTENNVSNLINDSIDQLKNGGEISDLINDI
                     VDHQTDMITGQNGGYVLLDPKEAPSRILIMDTPDKNTARNVLQINNAGIGFSKTGING
                     TYETAWTLDGGFNASFITAGEIVGITIRGTTLISDGADYRTSIANGKMTWYSKKVNKD
                     IMELEARDYVSADAGIVSYTMKTGGGFMIRNPQGNLVFSTWDNGNNRPFLSFGAPNFR
                     YSNASYVTSGDGSSLSINGSAGNSWEFKVAGRTMKFTSDGMLTLPGCFFGSWEDGKLA
                     RFEQSTVQVYKDFTVRGTKNSTVPTEHYGQRLLNAYETPEYYFADYGEAVTGDDGKVR
                     V"
     gene            complement(2061..2798)
                     /locus_tag="ACG8PO_002754"
     CDS             complement(2061..2798)
                     /locus_tag="ACG8PO_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFQ0208010.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFTLNDYVFSFEGLVVGTDIVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINHFNCTGLSKIQIYRNGLR"
     gene            complement(2849..>3679)
                     /locus_tag="ACG8PO_002755"
     CDS             complement(2849..>3679)
                     /locus_tag="ACG8PO_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFQ0208011.1"
                     /translation="SLVQAAATVIQNIWSTVSSWLGGIWNSISSTASNIWSSVTSSIS
                     NAINAAKSAIQSVWNSISSWISGIWNGIKNTALNLWNGITSTISSKVNDGKNAISSGW
                     SNLTGIVSDIFNNVKSTIANIWEGIKKTVSAPIDWIRDKISSVFDNLNISIPHIPLPH
                     FKLSGEFNPLKGKIPTLGVDWYAKGSVFNSPNIIGVGEAGPEAVLPLKRSVLQEIGDR
                     ILSSTSVSSRAQTIQPVNNYEFNFTIDGNADEVTMKQTTQQIIDSITKVQNDNASAWR
                     "
BASE COUNT         1110 a          779 c          548 g         1242 t
ORIGIN      
        1 tcaacacgaa ctttaccatc gtcacctgta acggcttccc cataatcagc gaaataatat
       61 tctggagttt cataagcgtt caatagtcgt tgtccataat gttctgttgg tacagttgag
      121 tttttagtac ctctaacagt aaaatcttta tatacttgta ccgttgattg ttcaaaccta
      181 gcaagtttcc catcttccca tgaaccaaaa aaacaacctg gtaacgttag cataccatca
      241 ctagtaaatt tcatagtcct accagctacc ttaaattccc atgagttacc cgcactacca
      301 ttaatgctta aagaactacc gtcgccagaa gttacataac tagcattgct atacctgaaa
      361 ttgggcgcac caaaagatag aaacggtctg ttattaccat tatcccacgt actaaaaacc
      421 aagttaccct gtggatttct aatcatgaaa ccaccaccag ttttcatggt gtatgataca
      481 ataccggcat cagcacttac ataatcacgt gcttctagct ccataatatc tttgttaact
      541 ttttttgagt accaagtcat tttgccatta gctatacttg ttctataatc agcaccatca
      601 ctaattaatg tagtacctct aatagtaatc cctactatct caccagctgt aataaacgag
      661 gcattgaatc cgccatctaa cgtccatgcg gtttcatatg ttccattaat accagtttta
      721 gaaaaaccaa taccagcgtt gttgatttgt aaaacattcc gtgcggtatt cttatctggc
      781 gtgtccataa tcaaaatacg actaggcgct tctttaggat ctaataaaac ataaccacca
      841 ttttgaccag taatcatatc agtttgatga tctacaatat cattgattaa atcactgatt
      901 tcgccaccat ttttcaattg atcaatggaa tcattaatca aattgcttac attattctct
      961 gtgttttcta agaagtttgt tttgacgttt cctacaacta atttatcgta tgaattggtt
     1021 agaacattaa acgtatattc cacaattctc gctgacatat tcacttttaa ctgtggatga
     1081 tacacatcta ctccgtcacc catcgaaact ttttctagat caacaaattt ttcatagcct
     1141 ctttgatgcc tcaatggtac taattcaatc gaaccactca cttgtggttt ttgtttatct
     1201 atgtttgttt tcaaccagtc tttagcagct tcccttaatg tggctacatc agtcgctttg
     1261 tctttaaaat caacaaaaga aacatatcca gcaggataat catccacgta atccgtgaaa
     1321 ataacttctt ctggtagagt gatctcgtct tctccttctg aagagctgct aatgaatgga
     1381 taaactccaa ctaaaacact ttgagcatca atctctaagt caagaccagt taagttttta
     1441 gtataaatcg ctttgatttt atgatccgta cctagacttt tttcatgacg taatgtgtta
     1501 ttatctttta gaaactcacc atgaaatcga tctagaatag atccctcttt tccaccaaag
     1561 aattctaaaa aattcgcctt ttctatcttc acattagcaa gcgtatctac taatgacgag
     1621 aaagaaaact gcgaagggat agctggtttc gctaaaactt ttgcgttttg ccatgcctga
     1681 gtagcagtga ttttttctgt tccgctgtca tatttattca acaccgattt tcttatatca
     1741 ttgaaaatag gttcagcttt tacttctatc gtattgccta ttacagaagt ttttgcataa
     1801 taaatccgta gacgctgttt tgctcgattt tcatctacat aacactgaat aatacgtcct
     1861 tctacaatca aatctgcatt agttccgttt attgaataag taccctgaaa tatctcggct
     1921 ccgtttagtt tattgctaac agtagctgtt aaccagtctg acaaagcgcc taaaccttgc
     1981 gtatcatata aatgttcagc taaattattc gcgtcatttt tatcgtaaat agttagtaaa
     2041 ttatcgatca tctatttcac ctaccttaac ccattacgat aaatttgtat tttgctcaaa
     2101 ccagtacaat taaaatgatt aatatccact tgcaacgttg gatattgcat ggtcttcatt
     2161 ttgttggacc gatctaaaat atctccgtcc gattgctctt cgtagcaaag catcaaatca
     2221 ctatcaatga ctatgtcagt tcccactact aagccttcaa aactaaacac ataatcattt
     2281 aaggtgaatt ggcatgaagt agctgaagga gtgatgataa tctttggaaa actttcttct
     2341 aaactattat tcagcaagtt aaatgactgt ggtttatcta cggttatagg tacatcttct
     2401 tgaactcttg cgaatggttt cgcagtgata tttacatcga actctcccca ttcaacaata
     2461 tcgttttctg catccccaat atcgatagtc tggataacat aatagacgtt gggatcatca
     2521 gagaattcta atttctttgc atagtttaac caatgacgca tgatataaaa cgattgcttg
     2581 aatgcttgat ggtcttccac atcttctaaa tagttatagt gcaatgtaaa cgacatgtct
     2641 tcaaacgagt aatcttgtac taagccacct agccgtccta aaacagaagt ttcaactctc
     2701 tgtctttttg gagaaggtat ggttggtctt tccgctaaag ccaatttatg caaataatca
     2761 ggaaatccat cgattataga atgtatacaa tcagtcattt tttcacatcc tttttaaaac
     2821 taaaaaaaca ggagaaatac tctcctgttt aacgccatgc cgaagcatta tcattttgaa
     2881 cttttgtaat gctatcaatg atttgttgag ttgtttgctt catagtaacc tcatctgcgt
     2941 taccatcaat tgtgaaattg aattcgtagt tattcacagg ttgaatcgtt tgtgccctag
     3001 atgaaactga tgtgctactc aagatacgat ccccaatttc ttgcagcaca gatcttttca
     3061 aaggtaaaac tgcttcaggt cctgcttcac cgacaccgat aatatttgga gaattaaaca
     3121 cactaccttt cgcataccaa tcaacaccca atgttgggat tttccccttc aatggattga
     3181 attctccgct caatttaaaa tgtggtaacg gaatatgtgg tatcgaaata ttcaaattat
     3241 caaagacact actgatttta tctctaatcc aatcaatcgg agcgctaaca gtctttttga
     3301 taccttccca tatattagct atcgtgcttt taacattatt gaatatgtcg gaaacaatac
     3361 ctgttagatt ggaccaaccg cttgaaattg cattttttcc atcgtttact ttagagctaa
     3421 tagtgcttgt aattccattc caaagattca aagcagtgtt tttgataccg ttccaaattc
     3481 cgctgatcca cgaagatata ctattccaaa cactttgaat ggcactttta gctgcgttta
     3541 tagcattgct tatactgcta gtcacgctac tccagatatt tgatgctgta gagctgattg
     3601 aattccaaat tccacctaac caactagata cagttgacca aatattttga attactgtag
     3661 cagctgcttg taccaagct
//
LOCUS       DAZKKW010000155         3670 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.157, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000155 DAZKKW010000000
VERSION     DAZKKW010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3670
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.157"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            10..618
                     /locus_tag="ACG8PO_002756"
     CDS             10..618
                     /locus_tag="ACG8PO_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFQ0208012.1"
                     /translation="MAKTKTVVTTFDNISIKRIAFNFKNAGNAIATNCNGQLDGETEM
                     QTVVKKCGAAEVKSKSKPINMTVTITAHVPMEVYRRFNGLKQDGRIKPGIYSYGPDSV
                     SEDFSLAAEIVDDFEENRKLVGMLACTSNTGLTFSIENGADEVAALELEAKVMQDEFG
                     KFYHEAIVAELEEDLTDQWMTNLSADVIKKTSVVATTATQSQ"
     gene            638..1000
                     /locus_tag="ACG8PO_002757"
     CDS             638..1000
                     /locus_tag="ACG8PO_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0208013.1"
                     /translation="MNEDYSKIELNDGTILNLEPKLNIKKLLMINRDFNTDEFAKMSM
                     GKGSMDITVIQGAKAVYVAYRQANMVDYISFDEFIDKWDFDMEVAVAVYSTMMFKQAR
                     DAYQKEFEKANKEKKLQK"
     gene            1003..1185
                     /locus_tag="ACG8PO_002758"
     CDS             1003..1185
                     /locus_tag="ACG8PO_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0208014.1"
                     /translation="MPKLLVETWVDVYSMLTDVFSMPSDLVLSDICLDDILQMAYNKS
                     AYEGWKNYAINQSQKN"
     gene            1203..>3670
                     /locus_tag="ACG8PO_002759"
     CDS             1203..>3670
                     /locus_tag="ACG8PO_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFQ0208015.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVQKLERELIN
                     QQTAQQRLSNDIDKTSKALAQSKGEFKTYESTMKDLDNEQQHLKSSASLVESEYKKWQ
                     ATAGQSATESEKLAKAQEYVGKQSDIAEQKIEVLKKQLDATQKEFGETSTEALEMKTK
                     LNDAEREFEELSNAAKNVDTSTVDDIGKKLDMGNLMEATDHLSVIGDKLIDVGSKSIE
                     AAGKAQAMQAQFKQVFGSLEGEAQDAVEGMAEEFGMLPNTIKPVFTQYTSMFKGLGYD
                     TKEAMELAGDSTQLAADAAAFYDKSMDDASESLNSFIKGNYEGGEQIGLFANDTQMAA
                     YAVKHNLIPATEGAKKASEESLLAVEKAQSKYADAIKKHGEGSLEAREAALKLKDAQD
                     KINEELGPQTQKWSDLDEATKQAVRVQYAEDMQKLAGATGQASRESDGLENQMTRAKQ
                     ALEDFYASLGEDILPVFIKGLQAGAKALQGLAEWWSKLDGPMKNFILALGGILALLST
                     LAPVITAVVTIVGTFGSTVLLPIIGIIAGVAAVIAIVITAFQNWGAITDWFSDLWKKF
                     TDWLGDTWESIKESASSVWDGVKETWSGFVDWVQEIWQGVSDWFGELWSGLVEGASNI
                     WQGVQETWQAFVDWVSNIWNGVKEVWSIIWADIVGIVQIPWTLITSLIQAGINIIVGI
                     FDVAGQLLGAAWQAVWTPISDFLKNTWDTMTQWISIAWNGIVTTFHTIFDPVVAWWNG
                     IWTSISTTASNIWNSISATASSIW"
BASE COUNT         1329 a          543 c          819 g          979 t
ORIGIN      
        1 ggaacaacaa tggctaaaac aaaaactgta gtaacaacgt tcgataacat aagtatcaaa
       61 cgaattgctt ttaattttaa gaacgcagga aatgcaatcg caacgaattg taacggacaa
      121 ttagatggcg aaacagaaat gcaaacggtg gttaaaaaat gtggagcggc agaagtaaaa
      181 tcaaaatcta aaccaatcaa tatgacggta acaattactg cacatgtacc gatggaagtt
      241 tatcgacgtt tcaatgggtt gaaacaagat ggacgtatta aaccaggcat ttactcttac
      301 ggtcctgatt ccgtaagcga agatttctca cttgctgcag agatcgtgga tgacttcgaa
      361 gaaaatagaa agttagttgg tatgttagca tgcacttcga atacaggatt aacattctct
      421 attgaaaatg gtgcggatga agtagctgcg ttagaactag aagcaaaagt tatgcaagat
      481 gaatttggta aattttatca tgaagcaatt gttgcagaac ttgaagaaga cttaacagat
      541 caatggatga cgaatctatc tgctgatgtg attaaaaaaa cttcagttgt ggcaactacg
      601 gccactcaat cacagtaaaa aaaacggagg tagcgaaatg aacgaagatt actcaaaaat
      661 tgaactaaac gatggaacaa ttttgaattt agaacctaaa ctgaatatca agaaattatt
      721 gatgatcaat agagatttta acacagacga gtttgcaaaa atgtcgatgg gaaaaggctc
      781 tatggatatt acagttattc aaggtgcaaa agccgtatat gtagcttatc gtcaagcgaa
      841 catggtcgat tacatttcat tcgatgaatt tatagataaa tgggattttg atatggaggt
      901 tgcagtagct gtatacagta ctatgatgtt caaacaagca cgtgatgcct atcaaaaaga
      961 attcgaaaaa gcaaataagg aaaaaaagct tcaaaagtaa aaatgccaaa gctcttagtt
     1021 gaaacgtggg tcgatgtcta ttcgatgttg accgacgttt tttctatgcc ttcagatttg
     1081 gttttaagcg atatctgttt agatgacatt ttgcaaatgg cttacaacaa gagcgcttat
     1141 gaaggatgga agaactatgc aataaatcaa tcccagaaaa actaaaggaa aggaggtaaa
     1201 aaatggctaa aaagagaacg gaagcagaag taactttcat agctaacgat gacggattga
     1261 aatctacgtt aaaagaaatc agtgctgaat taactaaaaa tagagcagaa ttaaaactag
     1321 aacaagctca attacaacag actggttctg aatcagacaa gttaggaagt aaattatctt
     1381 ctttagaaaa gcagtatgaa ttacaaagtc aaaaagttga agtaactagt caacgtttag
     1441 ccaatgccaa aaaatattat ggagaaaatt ccaccgaagt tcagaaactt gaaagagaac
     1501 tgattaatca acaaacagct cagcaaaggt tatctaatga tatagataaa acgagtaagg
     1561 cattggctca atctaaaggt gaatttaaaa cgtatgagtc aactatgaaa gacttagata
     1621 atgagcaaca acatctaaag tcaagcgcat cattagtaga atccgaatat aaaaaatggc
     1681 aagcaactgc tggccagtct gctacagaat ctgaaaaatt agcaaaagca caagaatatg
     1741 tcggcaaaca gagtgatatt gctgaacaaa aaatcgaagt gctaaaaaaa caattagatg
     1801 ccacacaaaa agaatttgga gaaacatcta ccgaagcttt agaaatgaaa actaagctta
     1861 atgatgcgga aagagaattc gaagaattaa gtaatgctgc taaaaacgtt gatacctcca
     1921 cagtagatga tatcggcaaa aaacttgata tgggtaattt aatggaagct actgatcact
     1981 tgtcagtgat cggagataag cttattgatg taggtagtaa gtctattgaa gcagctggaa
     2041 aagcacaagc tatgcaggcc caatttaaac aagtctttgg ctctttagaa ggggaagcac
     2101 aggacgccgt tgagggaatg gctgaagaat ttggaatgtt accaaatacg atcaagcctg
     2161 tttttacaca atatacgtca atgtttaaag gacttggata tgataccaaa gaagctatgg
     2221 agttagctgg tgatagtact cagttagcag cagatgcagc agctttttac gataagtcta
     2281 tggatgatgc tagtgaatct cttaattcat ttataaaagg gaactacgaa ggtggggagc
     2341 aaataggttt atttgctaat gatactcaaa tggcagctta tgctgttaag cataatttga
     2401 taccagcgac tgaaggagca aaaaaagcca gtgaagagtc attgttagct gttgaaaaag
     2461 cacaatctaa gtatgctgat gctattaaga aacatggtga aggatcttta gaagcaagag
     2521 aggctgcttt aaaacttaaa gatgcgcaag ataaaataaa tgaagaatta ggcccacaaa
     2581 cgcaaaaatg gtctgattta gatgaggcta ccaagcaagc agttcgagtt caatatgctg
     2641 aagatatgca aaaattagcc ggtgctacag gtcaggctag tagggagtct gatggtttag
     2701 aaaaccaaat gacaagagca aagcaagcgt tagaagattt ttatgcctca ttgggtgaag
     2761 atatactgcc tgtgtttatc aaagggttgc aagcaggagc gaaagctttg caaggattag
     2821 ccgaatggtg gagtaaactt gatgggccaa tgaaaaattt cattttagct cttggaggaa
     2881 ttctagcatt attaagcaca ttagctcctg ttataaccgc agttgttacg atagttggca
     2941 catttggttc tacagttttg ctgccaataa taggaattat agcgggcgtt gcagctgtaa
     3001 tagcgattgt aattacagcg tttcaaaact ggggcgcaat cactgactgg tttagtgatt
     3061 tatggaaaaa atttaccgat tggttaggtg atacctggga aagtataaaa gaaagtgcct
     3121 catcagtttg ggatggagtc aaagaaacct ggtctggatt tgtagattgg gttcaagaaa
     3181 tttggcaagg agtttctgat tggtttggag agttatggag cggattagtt gaaggagctt
     3241 ccaacatctg gcaaggagtc caagagactt ggcaagcatt cgttgattgg gtttcaaata
     3301 tttggaacgg agtcaaagaa gtatggtcga ttatttgggc agacattgta ggaattgttc
     3361 aaataccatg gacattaata acgtcattga ttcaagctgg tattaatatt atcgtgggta
     3421 tttttgatgt agctggacag ttattaggcg cagcttggca agctgtttgg acacctattt
     3481 ctgatttcct taaaaatact tgggatacta tgacacaatg gataagcatc gcttggaatg
     3541 gaattgtaac tacattccat actatatttg atccagtagt ggcatggtgg aatggtatat
     3601 ggacatctat tagtactacg gcttcaaata tttggaactc aattagtgca acagcttcta
     3661 gtatttggaa
//
LOCUS       DAZKKW010000156         3659 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.158, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000156 DAZKKW010000000
VERSION     DAZKKW010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3659
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.158"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            67..291
                     /gene="nrdH"
                     /locus_tag="ACG8PO_002760"
     CDS             67..291
                     /gene="nrdH"
                     /locus_tag="ACG8PO_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HFQ0208016.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            450..2609
                     /gene="nrdE"
                     /locus_tag="ACG8PO_002761"
     CDS             450..2609
                     /gene="nrdE"
                     /locus_tag="ACG8PO_002761"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFQ0208017.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFLSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYAMAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            2659..3624
                     /gene="nrdF"
                     /locus_tag="ACG8PO_002762"
     CDS             2659..3624
                     /gene="nrdF"
                     /locus_tag="ACG8PO_002762"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFQ0208018.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
BASE COUNT         1248 a          663 c          687 g         1061 t
ORIGIN      
        1 ttttttacta agagttttat actatctatt atagataata agaatgtgtt agaggaggca
       61 ctcaccatga acgtaaaaat cttttctaaa aataattgta tgcaatgtaa gatggtcaaa
      121 cggtttttaa cagaaaatca aattgctttt gaagaagtca atatcgatga acagcctgaa
      181 gcagtagact ggttgaaagc acaaggattc caaagtgttc ctgtcattac ttctgatgca
      241 gaaacagtta tcggattccg tcctgatcag ttgcgtaaac ttgccagcta acatgactaa
      301 aagaatcaaa acgtgataca tggatttgaa gggcttgaga cagcaccagt ttttgtcgca
      361 aacttccaaa tcctttttta aagcttaaaa acaatgcaat gatttgcaat caagcatcat
      421 caaatacgaa atgaaggttg ggcgtacgta tgagtcttaa aaatttacaa gatgtcagtt
      481 atttcaagct gaataatgaa atcaatcggc cagtcaatgg tcaaatccct ttgaataagg
      541 ataaagaagc tttagttgcc ttttttgagg aaaatgtcaa accaaatacg atgacttttc
      601 tatctattat ggaaaaaatc aattacttga tcgatcaaga atatatagaa gcagaattca
      661 ttcaattgta ttcaccatca tttattgaaa agctatatca atttttggac gagcaacatt
      721 tttctttcaa atcttttatg gctgcttaca aattttattc tcaatatgcg ttgaaaacca
      781 acgacggttc cgcttatctg gaaacttatg aagatcgagt agccttcaat gcgctttact
      841 ttgcgaacgg ggatgaagaa ttagcacttg ctcttgcaga cgaaatgatc catcaacgat
      901 atcagccagc tacaccttct ttcttgaatg ctggacgtaa acgtcggggc gaattggtgt
      961 cttgtttctt gactcaagtc actgacgata tgaactctat cgggcgctca atcaactctg
     1021 ctttgcaatt gtcccgtatt ggtggcggcg tagggattac tttatcaaat cttcgtgaag
     1081 ccggcgctcc tatcaaagga tatgagggtg ctgcaagtgg tgtcgttcca gtcatgaaat
     1141 tattcgaaga tagtttcagc tattctaatc aattaggtca gcgtcaagga gctggcgtgg
     1201 tttatttgaa cgttttccat ccagacatta ttgctttcct ttcagcaaaa aaagaaaatg
     1261 cagatgaaaa gattcgcgta aaaactcttt ctcttggcgt gatcgtacct gataaatttt
     1321 atgaattgac acgtaaaaac gaagatatgt atttatttag tccatatagt gtcgaaaaag
     1381 aatatggtgt cccattctct tatgtaaata ttacagctga gtatgatcat ctagttgcaa
     1441 atccaaacat tcgcaaacaa aaaatcaaag cgcgtgatct tgaaaatgaa atctcaaaat
     1501 tacaacaaga atctggctat ccttacgtaa tcaatattga tacagcgaac cgcgagaatc
     1561 caatcgatgg caagatcatc atgagtaatt tatgttcaga gattttacaa gttcaaacgc
     1621 cttctgtgat taacggcaaa caggaatatg aagtattagg aacggatatc tcatgtaact
     1681 taggctcgac aaatatcgtg aatttgatgc aaagccctaa ctttggtcaa tctgtgcgta
     1741 cgatgactcg tgcattgaca tttattacag atgcatctga tatcgatgtc gtaccaccga
     1801 tccaaaacgg aaataagctg aatcatacga ttggattagg tgccatgggc ttacacactt
     1861 atcttgcaaa agaacaaatg gaatatggct cagaggattc attagacttt acaaatattt
     1921 acttcatgct gttgaattat tggacacttg ttgaaagcaa caatatcgct cgtgaaagaa
     1981 aacaagtttt ccataatttt gaaaaatcag cttatgctga cggcacttat ttcgaaaaat
     2041 acgtaactgg agaattccag ccaaaatcag aaaaagtcaa agcattgttt gaaggcattt
     2101 ttatcccaac tgctgaagat tggaatgctt tgaaacaagc agtaatgaaa gatggactgt
     2161 atcatcaaaa tcgcttggca gtagcaccaa atggctcaat ttcatatatc aacgatacga
     2221 gtgcaagtat tcatcctatc actcgcatga tcgaagaacg ccaagagaag aaaatcggta
     2281 aaatttatta cccagcgcct tatctatcca atgagacgat tccgtattac acttctgctt
     2341 atgatatgga catgcgtaaa gtaatcgatg tttatgcaat ggcacaacaa cacgtcgatc
     2401 aaggcatgag tttgacttta ttcatgcgtt ctgaaatccc agaaggcatt tatgaatgga
     2461 agacaacaac gaagcaaacg acacgtgatt tgaatatttt aagacattac gctttccaca
     2521 aaggaatcaa atccatttat tacgtacgta cctttactga tgatgcagaa gaaatcggaa
     2581 gcaatcaatg tgaaagctgc gtgatctaat atcatagata gattcgctta ttcatatttt
     2641 aattaaagga gcattctcat gtcagaaaca tattatgaag caataaactg gaatgaaatt
     2701 gaagatatca ttgataaatc cacttgggag aagctgacag agcaattttg gctagataca
     2761 cgtatcccac tttcaaatga cctagatgat tggcgtacat tgtctcaatt ggaaaaagat
     2821 acagtaggac atgtatttgg cggtttgaca ctgctagata ctgtccaatc tgaaagtggc
     2881 atggatcagt tgcgtaaaga tgtccgcact ccccatgaag aagccgtttt gaacaatatt
     2941 caattcatgg aatccgttca tgccaaaagt tattcttcta tttttagtac actgaatacg
     3001 aaaaaagaaa tcgaagaaat ttttgactgg acaaatacga atccttatct gcaaaaaaaa
     3061 gctgagcgta tcaatgaaat ctacaaacac ggtacaccgc tagaaaagaa aattgccagc
     3121 gtctttttag aaacattctt gttctattca ggtttttata ctcctcttta ttatttagga
     3181 aacaacaaat tggctaacgt agcagagatc atcaaactaa tcatccgaga tgaatctgta
     3241 catggaacct acatcggtta caaattccaa ttaggattca acgagttacc tgaagcagaa
     3301 caagaaacgt taaaagactg gatgtacaat cttctttatg agctttacga aaacgaagaa
     3361 cgttatacag aagaattata cgatccaatc ggctggacag aagaagtcaa gacattccta
     3421 cgctataatg ccaacaaagc attgatgaac ctagggatgg atccactctt tccagatact
     3481 gccaatgatg tcaacccaat cgtaatgaac gggatttcaa caggcacaag caaccatgac
     3541 ttcttctcac aagtcggaaa cggctatttg ttaggtcatg tagaagcgat gaaagacgac
     3601 gattatttga ttggattgga ataaggattt attttaattc aaaaattttt cgaaaaata
//
LOCUS       DAZKKW010000157         3632 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.159, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000157 DAZKKW010000000
VERSION     DAZKKW010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3632
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.159"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1087
                     /locus_tag="ACG8PO_002763"
     CDS             <1..1087
                     /locus_tag="ACG8PO_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0208019.1"
                     /translation="DINDDGSRKKVEKVGGDTRPEAEAALRKVLSDIDETGQYFLGTD
                     TRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKLTDLSPALLQNFINA
                     EFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPKYEARPTTKKDLKII
                     SLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVDFSNGTITVEKQMVK
                     NDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYGKLYIDSNAVCTKED
                     GELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKMKEISERLGHSRISI
                     TMDTYSHVTDKMRNETVDIMENLRKNS"
     gene            complement(1305..2447)
                     /locus_tag="ACG8PO_002764"
     CDS             complement(1305..2447)
                     /locus_tag="ACG8PO_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0208020.1"
                     /translation="MRKIANVYKDRKRGTWFFSATLGQDRFGKRIQVTRRGFKTQGEA
                     RRGLEELKQSFSDSQGIYLQGITFHDFYREYFFPWYKLGTVEKTYMKTDHILQRALDF
                     FGEMQVVKIRPIHIQEFQQFLSKIHVVKKGIEQPLSPNYIKQIFNKLRVVFKRAIVLE
                     IIEENPVDTIGKIKTQRPQVEFWTVDEFKKVYNSVYHGDFYEEFYKRLMRFIFVTGVR
                     SEELLALQWKDFDLINGRCSVNKSIYIRTRQDYEFAETKNTSSIRIISLDRQTVEDLK
                     TWKKTQQSIGKNIELVFSFDGLPPSPRTILSRIKKLAEIAGVKPIHMHGLRHSHVAFL
                     IEHNKNIYAISKRLGHASIKTTLDKYGHLYPETNQALANEITRFDI"
     gene            complement(2452..2637)
                     /locus_tag="ACG8PO_002765"
     CDS             complement(2452..2637)
                     /locus_tag="ACG8PO_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFQ0208021.1"
                     /translation="MKTATRKDLINLGFSQYYANKIFKECKELLVEKGYFFYRNSKIK
                     RIPISILEEVLHISWEE"
     gene            complement(2991..3236)
                     /locus_tag="ACG8PO_002766"
     CDS             complement(2991..3236)
                     /locus_tag="ACG8PO_002766"
                     /inference="COORDINATES: protein motif:HMM:NF024059.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0208022.1"
                     /translation="MVDKSKKRQYLLVPLSIILSALEGDPISIKMILKHYHSYMAKLC
                     LTNGFNEVGQFVTYVDEYMISQLEIKLIEAILKFKIN"
     gene            complement(3230..3631)
                     /locus_tag="ACG8PO_002767"
     CDS             complement(3230..3631)
                     /locus_tag="ACG8PO_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFQ0208023.1"
                     /translation="MQQHYNDISIQHQFDAYCKKVLRNEAKSIRKRNANIKDNEEPLA
                     DSNEGRYSQTHFDEQDVYFLFGMEILISDQKLSAAIDQLSEIRRKIILLYYFAGFNDA
                     EIGKILNMSTSGIWYQRKKAVEQLKMGYDLW"
BASE COUNT         1189 a          653 c          561 g         1229 t
ORIGIN      
        1 agatatcaat gatgatggct caagaaaaaa agtggagaaa gttggcggag acacccgacc
       61 agaggccgaa gctgctttac gaaaagtttt atcagatatt gacgaaacag gacaatactt
      121 tttaggtacg gatactcgag taaaacaata ccttgatttt tggatggagg aatacgttaa
      181 actaaatcta aaatacaata cctatgaaaa ctaccgattt accatcaaaa atcatataaa
      241 cggttattta ggaaagaaaa aacttacgga tctctcccct gctcttttac aaaatttcat
      301 caatgctgaa tttaaaaagg gttactcgaa gaaaacaatg actattactc actctgtcct
      361 taagaatgcg ctgaatatgg cggtttatcc ttggggatta atcaagcaaa atcctatgct
      421 gtatgtaaaa ataccaaaat atgaagcacg accaacgact aaaaaagatc taaaaatcat
      481 ttctcttgag gactttgatc atatgctaga aattactcct gaaggacatc ctttctatat
      541 tcctttgaat attggatttt atacgggaat gcgcgttggc gaagtttgtg gtctgacgtg
      601 ggatgatgtc gatttttcaa atggaacaat tactgtagag aaacaaatgg taaagaatga
      661 cggcgcatgg gtatatggta cgccaaagac aagcagttcc aatcgaacga tttttattgg
      721 acaaaccttg ctagcaattc tgaaaaaaca taagaaacaa caattagaaa atcgaatgaa
      781 gtatggaaag ctctacattg attcaaatgc agtatgtacg aaggaagacg gtgagctagt
      841 tacgccaagt gtagtgaaat ggaatactag gaggatatcg aatgcactct ccctctcttt
      901 taacttccat tctctcagac atactcatgc tacacttctt ctcgaaaatg gcgcaaaaat
      961 gaaagaaatc tctgaacgat tgggccacag cagaatttca attacgatgg atacttactc
     1021 gcatgtaaca gataagatga gaaatgaaac ggtcgatatc atggaaaatc ttagaaagaa
     1081 ttcttgattt ttgccaccga aaaaaaccac cggtggcaaa ccggtggcaa atccatgtaa
     1141 acatggttta ttttttacac tgttttcaaa caaacccttg ctatttcttc ggttcgtcct
     1201 cgtccatttt cgataccgac agctagctta taacccaagg tatatcaaca taaaaaaata
     1261 aaaataggct atgacagcag cgtgacagca aaaaagatgg aaagttaaat atcaaacctc
     1321 gtaatttcat ttgcgagtgc ttgatttgtt tctggatata agtgtccata tttgtccaaa
     1381 gtagttttaa tagaggcatg acctagacgc ttagatattg catagatatt tttattatgt
     1441 tcaattaaaa aggcaacatg cgagtgtctt aatccatgca tatgaattgg ttttactcct
     1501 gcaatttcag ctagtttttt tattctgctt aaaatagttc ttggagatgg cggtaatcca
     1561 tcaaaagaaa aaaccaattc tatatttttc ccaattgatt gttgagtttt tttccatgtt
     1621 tttaaatctt caacggtttg tctgtctaac gaaattatcc gaatacttga tgtatttttt
     1681 gtttcagcaa attcataatc ttgtcttgta cggatgtaaa ttgacttatt tacgctacat
     1741 ctgccattta ttaagtcaaa gtctttccat tgtaacgcta agagctcctc actccttaca
     1801 cctgtaacaa aaataaaacg catcaaacgt ttataaaatt cttcataaaa atccccgtga
     1861 taaacactat tatatacttt cttaaattca tcaactgtcc aaaattctac ttgaggacgc
     1921 tgagttttta ttttacctat agtatctaca ggattttctt caataatttc aagtacaatt
     1981 gcccttttaa aaactactct caacttattg aatatctgtt taatataatt tggtgataat
     2041 ggttgctcaa tgcccttttt tacaacatgg attttcgata aaaactgttg aaattcctgt
     2101 atatgaattg gtcgaatctt tacaacctgc atttctccaa aaaaatccaa tgcacgttgt
     2161 aaaatatgat ctgttttcat atatgttttt tctacagttc ccaacttata ccaaggaaaa
     2221 aaatactctc gataaaaatc atgaaacgtt attccttgca gataaattcc ttgtgaatca
     2281 gaaaaacttt gttttaactc ttcgagtccc cttctagctt ctccttgagt tttgaagcct
     2341 cttctggtca cttgaattct ttttccaaag cgatcttgtc ctaaagttgc agaaaaaaac
     2401 caagtgcctc tttttctgtc tttataaaca ttcgcaattt tcctcatttt actattcctc
     2461 ccaagaaata tggagaactt cttctaaaat tgaaatagga attcttttta tttttgaatt
     2521 acgataaaaa aagtatcctt tctcaactaa aagttcttta cattccttga agattttatt
     2581 agcatagtac tgagaaaaac ctagattaat caaatctttt cgagtagctg ttttcattat
     2641 agcaccttcc ttactttttt tcatttattt ctatcagctg cttttgtcaa aagcacatag
     2701 gaatttcacc tctccccaag tttacggcga gccttacgga agtatcatta tcccaacttt
     2761 aaatcatcgc tttttattga ctataaaaat tattttagaa tatttatcgc tccttcttta
     2821 cagcaagaaa tcatggcgca actaaaatat gctccttaaa gtgttaacgt actgatagaa
     2881 tctatttaat tttcaatgaa caataggttt tctacctaaa agaatctact aattccttaa
     2941 ctattagaag attcttttaa acagaaaaag tggaaagggg atttttttaa ttagttaatt
     3001 ttaaatttta agatcgcttc tattaattta atttctagct gacttatcat atattcatct
     3061 acataggtaa cgaattgacc tacctcgtta aatccatttg tcaaacataa cttagccata
     3121 taagaatgat aatgtttgag tatcatcttg atcgaaattg gatctccttc tagagcagat
     3181 aagataatgg atagtggaac taataggtac tgccgctttt tagatttatc taccataggt
     3241 cataccccat tttcaattgt tcaactgcct tctttcgttg ataccagata ccacttgtgc
     3301 tcatattgag aattttaccg atttcagcat cattaaaacc tgcaaaataa taaagtagga
     3361 taatctttct tcttatctcc gataactgat caattgctgc tgataacttt tgatctgaaa
     3421 ttaatatttc cattccaaat aagaaataga catcttgttc atcaaaatga gtttgggaat
     3481 acctgccttc atttgaatct gccaatggtt cctcattatc ctttatgtta gcgtttcgtt
     3541 ttcgaataga cttagcttcg tttcgtaata cttttttaca gtaggcatca aattggtgtt
     3601 gtatagatat gtcattgtaa tgttgttgca tg
//
LOCUS       DAZKKW010000158         3629 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.160, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000158 DAZKKW010000000
VERSION     DAZKKW010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3629
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.160"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(247..924)
                     /gene="nth"
                     /locus_tag="ACG8PO_002768"
     CDS             complement(247..924)
                     /gene="nth"
                     /locus_tag="ACG8PO_002768"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HFQ0208024.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(931..1629)
                     /locus_tag="ACG8PO_002769"
     CDS             complement(931..1629)
                     /locus_tag="ACG8PO_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HFQ0208025.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            1897..3081
                     /locus_tag="ACG8PO_002770"
     CDS             1897..3081
                     /locus_tag="ACG8PO_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0208026.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQI
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            complement(3087..3530)
                     /locus_tag="ACG8PO_002771"
     CDS             complement(3087..3530)
                     /locus_tag="ACG8PO_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFQ0208027.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
BASE COUNT          939 a          723 c          608 g         1359 t
ORIGIN      
        1 tttatataat cctttggaat actggactta ttttataggt atattggaag gcattatttt
       61 ctacatcggg tattttccca ataaaaataa caacttttat ttttatcgca ataaattgta
      121 ataagttacc atcctagcta ctgagtatgt acatttactt tttcatgcaa aaaagataaa
      181 cgaaacagct tttctccata ataacaagat aattcataca tttatttgca attaaagaaa
      241 ccacgtctac aggcgtggtt tcttttttag cgccttttct tttagacgca tccgattttt
      301 cccatcttga catattgata gcaaaggaca tacctcgcac ttgggatttc ttgccgtgca
      361 atgatatcta ccaaaaaaaa tcagtgtatg atgtgtcttt acccataatt cttgtggtac
      421 tttccgcatc agtgtttctt ctacttccat gaccgtagca tctagtttgc agatcctcag
      481 gcgttttgag acacgttcca cgtgggtatc tactgcgatt gctggtatcc cgaatgcatc
      541 ccccaatacg acatttgctg tctttctgcc tacaccagga agagacatca attcttctct
      601 tgacgttggc acttgcccat cgaaacgttc aatcagctgc tgtgcacacg ctttgatgtt
      661 ttttgcttta tttcgataca aaccaatcgt ttttatttta agaatgattt catcgattga
      721 agcttctgct aacgcatcgg gtgtcggaaa agaggcaaac aaatctggcg ttgctttatt
      781 cactgataca tctgtcgcct gggcacttaa gatcactgca atcaataact caaatggatt
      841 attgtgcttg agttctccat gtgcttcggg aaacattcca tacatcgttt ctagagcttc
      901 cattgttttt tgtttgctta acatattccg ctactcctcc aaccaatttt tcagtgagac
      961 tttcggcaat tcttcttgtt gagctgactg ttctacttct ctttgaagaa gctgctgttt
     1021 tcttcgtttt tggtcttctt ccacttgttg ttttgtacgt agattttttc tttcccacga
     1081 tagcaaaatg cggtcaacgt aattaaaact ataagcctga tttaatacgg cttcccgcag
     1141 tgctagcttc aaaatttccg gctgataatg gtcttcttct agccattgtc cgatccgctg
     1201 gaactcgatc gaagaaagcg ggcgtccaaa ttcttgttca aataattgat acactgactg
     1261 gatttctttt tcttgtgttt tttcttcgta ttttttttct tgtgttttta aatactcacc
     1321 taatagatca tagatggggt aaagattata tcgatctgct tttttaccgt tgacaatcgt
     1381 cgtttctatc ttgataaatc catttgttac gagacgatcc agtatttgaa aaatctgatc
     1441 ttgtttgatt cccatatcca tagcaatcat ctgcaaatca gggaaagtat ctccttcaag
     1501 ctgtgccata tgcaactgta gaacaaataa aaattcttca gattgtagac cgatcctatg
     1561 ataattttta agtaaaagat tagatatgac tgtttgtcca gcgcttaagt aattttctaa
     1621 attcaacatg atttctcctc cgctcaatca cagtatacgt aaaaaaaaca tgggaagcaa
     1681 gaacaggttt cttttctatt tacttcactg atcggtcaga tttacaatgt cgtctacttg
     1741 atttttgttt gttcatgttt ttgatgcgta tcttcacttt tcggaataaa taaattaagt
     1801 agatttcgct gttccttaga atgttgacaa ggaaacaaaa ttactataca ctatttttgt
     1861 ttccaagtta acattattgc ataaaggagt ttttacatgt acagtttcac aaataaacat
     1921 tctaaagttt ttggatacgt ctctgtctta ttcactggat taggatttac tatcgtcagt
     1981 cctgtacttc cgtttttaac gctgccttat tctcactcca tacatcagca agctttctat
     2041 atcactttgc tcatgtctgt ttatgctcta gctgccttct tatctgcccc cattctcggt
     2101 agcttaagtg atcattttgg gcgtcatccg atccttatta tcagtttatt aggatcaagt
     2161 atcggatatt tgctttttgg tttaggaaat tcgatatgga tgttgttcct tggtcgtatc
     2221 attgaaggat tgacggcagg agaaatctcc accttatatg cttactttgc cgacatcacg
     2281 gaacctaatg aaagaaccaa agtttttgga tggatgggtg ctttagtcgg tataggaaca
     2341 acgctgggtc ctattatagg gggtctatta gctgagttgg ggaattctgt tcctatattt
     2401 attggagcac tctttacttt cttaaatgcc gtttatggct acacatttat gctagaaagc
     2461 cttccaataa aaaaacgttc agtagattta agtttctccc atgtaagacc ttttcaccaa
     2521 ttacaacaat tattcaagat ttcatccgtt attcctttat tgacagcagg ttttgctgtt
     2581 tggttggcag ctggctctct tcaatctatc ttttctcaat tttcgattga cgcctttcag
     2641 tggaaggctg ggttggttgg tttaagcttc tctttgattg gaatcttgga tactgtctcg
     2701 caattgttca ttatgcctcg attgctcaaa aaattttcag aacagcagat cacaagaata
     2761 gggatgttca gtgaaatcct cgcctatcta ttcatcacat tatctggaat tcttctactg
     2821 ccgattttgt ttttatttgg gattatctgt tatggtttcg gtgattctat ttttactcct
     2881 gtattcaatg gccaaatatc caattctgtc tcagataacc agcaaggtct tgtaatgggc
     2941 ggtacccaaa gtatacaatc tctatcgcgc gtcatcggcc cgctaatcgc aggacagctt
     3001 tatgctgcag caccatttct tccgacggta ttcggatttt tgcttatgat ttttgcctat
     3061 tatcactaca ataaaaaata gtagcttcat ttgctacttc gtataaattc tctgtatcga
     3121 ccgataaaac gaaatactgt gtcaaattct tcttttgaca tattttcaaa tacagtagca
     3181 tctcgctgta cccatttttg atggagttgc tcgtgtctat tccttatttt ttgtccgatt
     3241 ggcgtcaatt gaaaatagat ttctttttta ttctcttcgc tttgataagt gtttatgatc
     3301 cctctagcta tcagtttttt tgttagctta ctgatggctc ctctagtcat gtaactagca
     3361 ttcgctaatt ttgtcacgtt aggatcaggg attttcccga tatgttcaat cacttctagt
     3421 tcatttagag taaatcctgc aaattctttt tccatttctg gtttattacg atgaataatc
     3481 tcattagcta aatccagata ttcttctatt agcagttgtc ttttatccat ttctacacct
     3541 tctttttctt tcattatatt gtttttttgt ttcctattca acaaagataa aaatattttg
     3601 taggaaaacc caatctgaaa ccaagaaaa
//
LOCUS       DAZKKW010000159         3628 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.161, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000159 DAZKKW010000000
VERSION     DAZKKW010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3628
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.161"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            72..1040
                     /locus_tag="ACG8PO_002772"
     CDS             72..1040
                     /locus_tag="ACG8PO_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFQ0208028.1"
                     /translation="MANEIVKYHHELNTIPLRKFTSVEMNLFFSIISRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKADIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGIA
                     NFSKEDFHELLDIPKSYRESDINKKIIKPIRQELTAIFKGLTIQKRYGKGRGKPVIGY
                     QFTFKPEIKKSDDFNKHAPTKEEQNKALELANQQVQSEKEPTSLQEKIKEQKRKLELL
                     QQLAELERKQNTPSKKINKQIADNNSDITPEFKENLLDNLQNLFKE"
     gene            1050..1538
                     /locus_tag="ACG8PO_002773"
     CDS             1050..1538
                     /locus_tag="ACG8PO_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFQ0208029.1"
                     /translation="MTDKTIKELADILGVSKDKVKYQLRKLPSNFTYKKGNITYLKKE
                     AVTTITKVLSGNESGNLPSELPDSLPTDYLLKEIETKNNHIEEKDRQIEKLQIALETQ
                     QKLLDQQQQLTLQANQQIKKLHEQLQLTYAEESSEKEPLSVTEETQKGQNKEKKWWQF
                     WQ"
     gene            1834..2115
                     /locus_tag="ACG8PO_002774"
     CDS             1834..2115
                     /locus_tag="ACG8PO_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0208030.1"
                     /translation="MDILVRNIDPKVISEIDRRCQELTTKTGKKWSRNAYLKVLIEND
                     FDQALRHYKKDQFDLLLEKFIDIQAKNTETLNDYVHTNNQLIETLIGGI"
     gene            2117..2533
                     /locus_tag="ACG8PO_002775"
     CDS             2117..2533
                     /locus_tag="ACG8PO_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0208031.1"
                     /translation="MRKTSVQKHVTLYPSHIDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPNTNTTTTSNQAKLNAMGKEISIMSEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     ENQLNPSRRNQPKNRQKETNQTFTSRNFDPTNFLKR"
     gene            3147..>3628
                     /locus_tag="ACG8PO_002776"
     CDS             3147..>3628
                     /locus_tag="ACG8PO_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphopain proregion domain-containing protein"
                     /protein_id="HFQ0208032.1"
                     /translation="MIILKPKKKIYLGLLFSLMIGIFSIGITNTYASENSNFNINSQP
                     IEEQAINFAKTDFEHSITSILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFS
                     DADNTIKYVYTVKKNTNGEYSGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSDNG
                     "
BASE COUNT         1449 a          616 c          535 g         1028 t
ORIGIN      
        1 attagggggt tgacccccat tagaaagctc tttataattt tgttaagtag ttaatcttga
       61 aggaggattt tatggcaaat gaaattgtga aataccacca tgaattaaat acgattcctt
      121 tacgcaaatt tacgtcagta gaaatgaatt tattcttctc tataatatct agaatgcgtg
      181 atgtaggcga taaaaaagta caatttacat tcgaacaatt aaaagattta agcaattata
      241 aagcaactgc aaatgttcga ttcatagatg atttagaaac aacgtatgac aaattaatgg
      301 atttacgttt tggtagaaga agtgctgacg gtttgcaacg tgaacgattt gttttattta
      361 atcaatttaa aatcgatgga aaagctgata ttccttttgc tgaaatacaa gtccatgaga
      421 aagccttacc cttacttaat aatcttgaag aatgggttcg atattctctc caacaattca
      481 atgaattaga aagcagttat tccaaaacca tgtttagatt attaaaacaa tacagaacca
      541 aaggaattgc caatttttca aaagaagact ttcatgaatt acttgatatc cctaaaagtt
      601 atagagaaag tgatataaat aaaaaaatta taaagcctat tcgccaagaa ctaaccgcaa
      661 tcttcaaagg tttgacaatc caaaagagat acggaaaagg acggggaaag cctgtcattg
      721 gctatcaatt tacatttaaa cctgagataa aaaaatcaga cgactttaac aaacatgctc
      781 caaccaaaga agaacaaaat aaagctttgg aactagctaa tcaacaggtt caatctgaaa
      841 aagaacctac ttcattacaa gaaaaaatca aagaacaaaa acgaaaatta gaacttttac
      901 aacagttagc agaattagaa agaaaacaaa ataccccaag taagaaaatt aacaaacaaa
      961 tagcagataa caattccgat attaccccag aatttaaaga aaacttacta gataatctcc
     1021 aaaacttatt taaagaataa ggggttttta tgacagataa aactatcaaa gaattagcag
     1081 atattctagg tgtttcaaaa gataaagtga agtatcaact gaggaaatta cctagtaatt
     1141 ttacctacaa aaaaggaaat attacctacc tgaaaaaaga agcagtaaca acaattacaa
     1201 aggttttatc aggtaatgaa tcgggtaact tacctagtga attacccgat tcattaccta
     1261 ccgattacct actcaaggag attgaaacta agaataatca tattgaagaa aaagataggc
     1321 aaattgaaaa acttcaaata gctttagaaa cgcagcaaaa attactcgac cagcaacaac
     1381 aactcaccct acaagccaat caacaaatca aaaaacttca cgaacaactc cagctaacct
     1441 atgcagaaga atcttcagaa aaagaacccc tttctgttac tgaagaaact caaaaaggac
     1501 aaaacaagga gaaaaaatgg tggcaattct ggcaataacc caacaatttt tattcataaa
     1561 aattgttggg ttactttctt tttttgcttt tacaagcata gcgacatgat ctcacgtcgc
     1621 tattttttaa agagcataag gaaacgcggt atcactttct tacgaaaggt gctaggtagg
     1681 ctgtgcctac cacgtttcta agaaaaaaat ttgctatcac tttttcgtca ttgtggatag
     1741 cttatgtata gtgtttcggc aaaaaacagc tatacaaata ctatatttgt atgatataca
     1801 taaattatac acaaatgcga aaggaattct tctatggaca ttttagttcg gaatattgat
     1861 ccaaaagtta tttcagaaat agatagacgt tgccaagaac tcacaacaaa aactggcaaa
     1921 aaatggagcc gtaatgcgta cctaaaagtt ctaatcgaaa acgattttga ccaggcactt
     1981 aggcattaca aaaaagatca attcgattta ttactcgaaa aatttatcga tatacaagca
     2041 aaaaatactg aaacattgaa cgactatgtt catacaaaca accaactcat tgaaacactt
     2101 atcggaggaa tataacatga gaaaaacttc tgttcaaaaa cacgttaccc tttatcccag
     2161 tcacattgac gttttagatt ccatcatcga aaaaaatcca gatatcaata actactcgga
     2221 agccattcgt tttctatgta tgagttatga acctaatact aatacgacaa ctaccagcaa
     2281 ccaagcaaaa ctaaatgcga tgggaaaaga aatttctatt atgtccgaaa tcattgcaga
     2341 atttgctgat ttatatctaa aagatactgg tgttcttatc ggtacaaatt ctaaagtata
     2401 tcaagaagtg aaagaaaaaa ttgaaaatca actcaatccc tcccgacgaa atcaacccaa
     2461 aaatcgtcaa aaagaaacca atcaaacatt cacttctaga aattttgacc caacaaactt
     2521 tctaaaaaga tgaaaaagaa accgaaaatt ttttctattt ctcagtttct tttttagggg
     2581 gcagtcttcc agcaagattt gcaagttacc ggtaacttgc acaaaaaaag aaaaatccgc
     2641 ttgtgcgaat ccttcttttt gcattttggg gcgctgcccc aatctccaaa tatcggttat
     2701 ttaatagcgt ggtaactcaa aaaaatagaa cgtactctga aataaaaaaa gactgctcta
     2761 acgagcagcc agttaaaaaa taatgagtgt ttattcttga aaaagcacgc gcttatgaag
     2821 cgctaccaaa aatttaccat attttacaaa aaataacaag tcttaaaaaa atgacagata
     2881 aaaatgcaca aagaattaga tatagataac cgcgaaaaat caagctttag aaagttgctt
     2941 ttagctagac caattaaaaa tcacaaagtg agcgtctcta tccattggta gaggggctac
     3001 ttggcatagt gtacattatg tctactagct cgataaataa cgattatgtt cccaaaacaa
     3061 tattttatga actataacaa aaatttgtta tcgcatattg tatattttaa ttataggtgt
     3121 atatcactta aatttaacac aaaggcgtga ttattttgaa acccaaaaag aaaatttatc
     3181 taggtctcct cttttccttg atgattggaa tttttagtat aggaattaca aatacctatg
     3241 cttcagaaaa ttctaatttc aatatcaact cccaaccaat tgaagaacaa gcaattaatt
     3301 ttgcgaaaac tgattttgaa cactccataa cttcaattct tttaatggaa aataacaccg
     3361 aaactgatat taactatact cttggttcgc catttaaaat gctcgatgga gaaaatacgg
     3421 caaatagttc tattttcttt ccagtatttt cagacgctga taatacgatt aaatatgttt
     3481 ataccgtcaa aaaaaataca aatggagaat attcaggaag tatttcacaa tttttagcaa
     3541 aagaacttca aaatctagta gatagtaata taacagatac aaccttattt tttcaagata
     3601 atagcattta ttattccgat aatggaca
//
LOCUS       DAZKKW010000160         3552 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.162, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000160 DAZKKW010000000
VERSION     DAZKKW010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3552
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.162"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            137..748
                     /locus_tag="ACG8PO_002777"
     CDS             137..748
                     /locus_tag="ACG8PO_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFQ0208033.1"
                     /translation="MYFILPTLYSLLCVMAPCIIYMVFFCKKNTLKHNILILIFMLYC
                     WKVFELTGTGGLTDIFYRPDTGRKFSLVQGSVNLVPFSGLNISFILNIVMCVPLGFLL
                     PIIWKSYRKIVPVFLAGALFSLIIEVSQIITTRATDIDDLIANTFGAVIGFILWAVLS
                     RVVSLKDKSSTVDYLEPYIYILIGFTGKFLLYFPFWFAFEVLS"
     gene            821..1750
                     /locus_tag="ACG8PO_002778"
     CDS             821..1750
                     /locus_tag="ACG8PO_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HFQ0208034.1"
                     /translation="MKRFLKWGLVLLASLIVILMCTAVVISYVNRPYARAVYDRIKRI
                     LDKDFYDHLYLGSSFEIINLDTMDIHSYMKKDQEELPASLSKLFTIDYALSVLSLDEQ
                     ITISPTALDMQKADSSTAHLQEGDILSVYDIICAMVLPSGNDAAYSLAIYTGGKLDPD
                     AKNDEERYQSFMKNLDRYLSEQGYKHTHFTDAAGYDSNATTSAEDLTKVAAKLLKNDK
                     FAEIVAKETYRAKSIKGNVYNWNTTNQFMQQDNRYFTEGVKGVKTGSFDGRYNLCVYY
                     TSSSDNHYLLVSFGSESDSRRYVDVQNLLHNLP"
     gene            1887..2645
                     /locus_tag="ACG8PO_002779"
                     /pseudo
     CDS             1887..2645
                     /locus_tag="ACG8PO_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033645.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VirD4-like conjugal transfer protein, CD1115
                     family"
     gene            complement(2693..>3552)
                     /locus_tag="ACG8PO_002780"
     CDS             complement(2693..>3552)
                     /locus_tag="ACG8PO_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFQ0208035.1"
                     /translation="FRKGITMSEIADLIEKMYGHHYTPQTMSNMTKSFTEEVTSFKER
                     ELHDRYAAIYMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEE
                     ILIDLQERGVKNVLLFITDGLKGMAGAVQRFYPKARFQHCCVHVSRNISHKVRVDDRK
                     EVCDDFKMVYQASSKKAALEARGAFAEKWKTSYPKMVESILSNDYLLTFYDFPLAIRK
                     TIYSTNLIESFNKQIKKYSHRKEQFQNEESLERFLVSIFDTYNQKFLNRSHKGFQQVT
                     DTLASMFTE"
BASE COUNT         1170 a          555 c          682 g         1145 t
ORIGIN      
        1 cctctaggaa aaaattaact agcaccacgg gttatgatat aataaaatgt attataggca
       61 tttttttaca agaaatagaa gcttgaagtt tatatgaagt tataaaattt aatttatatt
      121 ggaaggagga aaataaatgt attttatact acctacactt tatagccttt tatgtgtaat
      181 ggcaccatgt attatttata tggtattttt ttgcaaaaaa aataccctga aacacaatat
      241 cctgattttg atttttatgc tgtactgttg gaaagttttt gaattgactg gtacaggtgg
      301 actaacagat attttttata gacctgatac aggtaggaaa ttttctctag tacagggcag
      361 tgttaatctt gtaccattca gtggactaaa cattagcttt atattaaata tagtaatgtg
      421 tgtgccctta ggattcttac taccaatcat atggaaaagt tataggaaaa tagttcctgt
      481 tttcttggct ggggctctat tttcactaat tattgaagtc tcacaaatta taactacaag
      541 agcaacagat attgatgatt taatagcaaa tacctttggt gcggttatag gctttatact
      601 gtgggctgtt ttaagtagag tggtgtcctt gaaggacaag tcatctacag tagattattt
      661 agaaccttat atttatatat tgattggttt taccggaaaa ttcttactgt attttccttt
      721 ctggtttgct tttgaagtat tatcctgata attccataga aaataatgag tttgagttga
      781 gaaagaatat tcttgtacac aactctggaa aggaggaagt atgaaaagat tcttaaaatg
      841 ggggcttgtt cttttagcgt ctttgattgt gattctaatg tgtacagcgg ttgttatttc
      901 ttatgtgaat cggccgtatg caagagctgt ttatgatagg attaaacgca ttcttgacaa
      961 agatttttat gaccatctat atctaggttc ttcatttgaa ataataaacc ttgatacaat
     1021 ggatattcat agctatatga aaaaagacca agaagaatta cccgcctcat tgagcaaatt
     1081 atttacgatt gattatgcgc ttagtgtcct aagtttagat gaacagatta caattagtcc
     1141 tactgcactt gacatgcaga aagctgactc atctactgca catcttcaag aaggagatat
     1201 tttatctgtt tatgacatta tctgtgctat ggtcttaccc tcaggaaatg atgcagctta
     1261 tagtttggca atttatacag gtggaaaact tgatcctgat gcaaagaatg atgaagaacg
     1321 ctatcaaagc ttcatgaaaa acttagatcg ttatctaagt gagcaaggct ataaacatac
     1381 tcattttact gacgctgctg gctatgactc caatgctaca acatcagcgg aagatttgac
     1441 aaaagtagct gcaaagctac taaagaatga taaatttgca gaaatagttg ccaaggaaac
     1501 ttatagagca aaatcaatta agggaaatgt ttataactgg aatactacga atcaatttat
     1561 gcaacaagat aatagatact ttactgaagg tgtaaaaggt gtaaaaactg gatcgtttga
     1621 tgggagatac aatctatgtg tgtattatac atctagttct gataaccact atttacttgt
     1681 aagttttggt tcagagagtg atagtaggcg ttatgtagat gttcaaaatc tattgcataa
     1741 tctgccttga aatttcgtgc ctttaagcgc gtatctaaag tttctttgaa aagtttgaga
     1801 atgaagaaag tgagcagtcg aaaaacggtt gctcactttt ttgccctaaa atcgaaaaaa
     1861 tataaaaaag tcctttacaa cacgtctcag gaaaaactgc taagtcaatt ctaatatctt
     1921 gtggagcaag acttgcacct tttgatataa gagagcttag agaactaatg agcgaagatg
     1981 aactagaact cgataaaatt ggagatagaa aaactgcctt gtttgtaata atatcagata
     2041 cagatgatac atttaatttt gtagtttcaa taatgtattc tcaattattt aatctactat
     2101 gtgataaggc agatgatgtg tatggtggaa gacttccagt tcatgttagg tgtctacttg
     2161 atgagtttgc aaatatcggt ttgattccta aatttgaaaa actaatagcg acaattcgtt
     2221 caagagaaat atcggcaagt ataatactgc aagcacaatc tcaattaaaa gcaatttata
     2281 aagaccatgc agatacaata gttggtaact gtgactctac actttttcta ggaggaaaag
     2341 aaaaaacaac agtaaaagaa ttatcagaaa ccttaggaaa agaaaccata gacctatata
     2401 acacatcaga aacaagatca aatcaaaaat cttttggtct aaattatcaa aagactggca
     2461 aggaacttat gagtcaagat gaaataactg taatggatgg tggtaagtgt atttaccagc
     2521 taagaggagc aagacctttt ctatctgata aatttgatat tacaaagcat aagaattaca
     2581 aattgttgga agattatgat aagaaaaact tgtttgatgt agaagagtat ttaaaacaaa
     2641 gatagcgtaa aatattttgt gtaaataact cgtccttctg caaaataatg ggttactcag
     2701 taaacattga agctaatgta tccgtcactt gttggaagcc tttatggctt ctatttaaga
     2761 atttttgatt gtatgtatca aagatggata ctaggaaacg ctctagtgat tcttcatttt
     2821 gaaactgctc ttttctgtgg ctgtatttct tgatttgctt attaaaggat tcaatcaagt
     2881 tcgtagagta aatagtcttg cgtatggcta atggaaaatc atagaaagtg agcaggtaat
     2941 cgttcgaaag gatcgattcc accatttttg gataactggt tttccatttt tccgcaaaag
     3001 caccacgtgc ttccaatgcc gcctttttag atgacgcttg atacaccatt ttaaaatcat
     3061 cacagacttc cttacgatca tcgacacgca ctttgtgact gatattacgg gaaacgtgca
     3121 cacagcaatg ttgaaaacgg gctttggggt agaaccgctg cacggcccct gccatcccct
     3181 ttaagccatc cgtgatgaaa aggaggacgt ttttcacgcc acgttcctgg aggtctatta
     3241 aaatctcttc ccaaatcgtg atagattcag ttggtgcaat cgcatagctc aacacttcct
     3301 ttgaaccgtc cgggcgaatg ccaactgcga tatgaatggc ttccttggcg acggttttcc
     3361 gctttaaagg gatataggtt gcgtccatat aaatagcggc ataacggtca tgaagctcac
     3421 gttccttaaa tgaagtgact tcttccgtaa atgatttagt catattggac atggtttggg
     3481 gcgtgtagtg atgcccatac attttctcaa tcaagtctgc gatttccgac atggtaatac
     3541 cttttcggaa ga
//
LOCUS       DAZKKW010000161         3433 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.163, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000161 DAZKKW010000000
VERSION     DAZKKW010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3433
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.163"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..2177)
                     /locus_tag="ACG8PO_002781"
     CDS             complement(<1..2177)
                     /locus_tag="ACG8PO_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prealbumin-like fold domain-containing protein"
                     /protein_id="HFQ0208036.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTSNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVL"
     gene            complement(2849..3117)
                     /locus_tag="ACG8PO_002782"
                     /pseudo
     CDS             complement(2849..3117)
                     /locus_tag="ACG8PO_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
BASE COUNT          971 a          756 c          540 g         1166 t
ORIGIN      
        1 aggacttcac cactgccttc agcatgttta attagttcaa caccacccgt atcaaggatg
       61 ttttcaatgg ttaacgtcac ttcttctatg gagtattcat ttataaaaac atcatgtacc
      121 gtcgcatcta aaagataccc ttccggtgct tctgtttctc ggacttgata gctacctaca
      181 ggaagcggtt cacttaaaaa ttctccgttt tctgccgtta taccttcttg tatcacttgt
      241 gaatcgcttg ataaaatttc aaattttgct cctgccagcc tttggccatt ctcatcagtt
      301 ttaattactt taatacgccc atcgattttc cttgttctta cagtactcgt attcaatcga
      361 ttgtcactat tcacaagcgt tgcaaagttt aaaatttgat cgccgaccgc atacgcctct
      421 acattgacct taaaagagag ggtaaaactt gtcggaccac caatcgtatt attcgcactg
      481 aaacgaacta ttccattatt gacttcggca tcaatgaaag agggaatatc tgatagaaat
      541 gacacaaatt ccatgccttc ttccaaaaca tcttcaataa caagcgtatt gcttgttcca
      601 gaaaacatcg agacatgcag agtatacgtt agttcatttt cattgagatt gataacattt
      661 ttgtccacac tttttcttaa agagattgcc ggaagattac tttcagggat tctcaagcgg
      721 tagtctcggc tcattacctg attattgtag gtcatactcg ctcgattccg agaaaaccca
      781 tcctcttgtc ttccttcatt gaacaagtcg acatcggtca cttcaagagc atattcaata
      841 aaatacgatt gaccctctgc tagattacca aagtccacaa caagtgtatt cgtttcctta
      901 ttaaaagata ctttgtccac aaattcctcg gaaacataat gagttctaac gtttgcttca
      961 cctggattcg ttacttctgc gatccgaacc ccacctgaaa taggttctct atttccagtg
     1021 gaactccatg tagtcggatt cggtgccaaa gttgttcctt gcggtagtac atcggtaacc
     1081 actacaccgt cttttcgtga tccttcataa tttacctgca aaataaattt attactgttg
     1141 gtcacgtctt gcccgtcaat cagcagattc ccaagatttc cttggctgtc gatttctcta
     1201 gagccatagt ataatttcga aaaaatgggt cttgatgaag gaataggcgt aattgaaaat
     1261 tccgtgtttg ctgctcgctc agcataagta agactcactt gaaagtcatt atttggctca
     1321 gtaccgcctg attgaaagaa agcaacttca aacgtgatac tgaatgtata cgactgagcg
     1381 gcttcattta atccttcata atcaaaggta aatggaaaaa caacgtctgt gccttcttgg
     1441 taaaatgcca gtggctttaa aggtgaatca tcagcgacct ccactctaat gaactcaatt
     1501 aagccaatcg atgctctagg aactcgtacg accatctccc catattcttc aagaatatcg
     1561 ccagcgcctc cttgaatgta tccctttaac gtttgtgtat gcctgctcgg caaagtgggg
     1621 cgctcagcag agatgccgat gtcataattc aagagctcct cttgtctttc aatctgagat
     1681 tcctgcgtta tagatggctc agatgatctg gcttcattaa aagattcact taagctttct
     1741 tctggtatat taaaagattc acttgtgctt tcttctggta cattaaaaga ctcacttgtg
     1801 ctttcttctg gtacactgat ttctattgag accgtatttg attgaatatc accgcttctt
     1861 gcggtcaatt cgtatttacc tgaatgctgg gaagaataag taatcagacg ttcgtaatca
     1921 ttgagttgct tcacttcccc ttcaatcaaa tcctcactct tatcaatgac caatgcattt
     1981 agcaggtctg gtgttcctct gactcgcaac tggaactgct cacccaaagc agcctcctct
     2041 tgatcaactt cgatcgtcag cgaacgctct ggctgttctg ctaaagactc agctagaacc
     2101 gttatgttcc caaattgatt gatcacaata agcataacta gaaacatact taacagttta
     2161 tgaaatttgt tcctcactat tactccccct tataattttg actaaacaac aaggtacccg
     2221 ataattttct aaaaagattc cccaactcaa aatggctgcg ttacctccct cgtattttcg
     2281 gttcaactca agcgttgcaa tgtaagctgt aaacaaagct aatgattgcc gattgtcttc
     2341 tgttgattga ttcgatagct ttgtatgact tttagttgtc cgtgtggctt aaccataatt
     2401 atgatttcac gattcctcct gccagtgcct tgcacaagct ggagcgtaac cctaatagtt
     2461 attttgttaa tgtacttata tacttttata cttttccatt agaaacaatc actaaatcgt
     2521 tgataccaag gtcagactct agaatttcat cagtgagtcc ctgtattggt taaccttcat
     2581 caatcccaaa aaaatatatc aatgcttagc atcaaaatga atatatagtt tagaatactt
     2641 gggattcatc gataactgat cgcttatttg gccatattat ttggcacttg gttcaactaa
     2701 acgcatttag gatgttcctt cattctcgtc atggatgttc tcaaaccgtc attactaata
     2761 aactgtccac cgaatcgatg gttcatttct tgcattttcc acgtccaatt actctttttg
     2821 ctctttattt gggcgtagtt tcactttctt aacttgtatc gtcttccatc aactccttaa
     2881 tcatcttctt agctttattg gctcggtttc cttgtaaaag gtatctacaa atagtgacaa
     2941 tctgtattaa atcttcatca ttttctgtaa tgtcaataat ttctatattc tgtaccatat
     3001 attgcaaagg gtttcactaa gttcataacc gaagcgaatt aaacaacttt tgtatagaat
     3061 aactaccttt tctatttttc cattggtaat tctatctatt aattggttta atccttttgt
     3121 tgtagttgat tccactacca agatgattct ctcgaatttt aaagcttgaa gatgtattgc
     3181 tatttttgct aactggcgta tttgcaacta tttttccctt ctattcttcc ttcattgatt
     3241 cccctctaaa attactatcc ataaaaaatt gcataaatga caatagtatt tatattagtc
     3301 atactgcttt ttaacacagc aaattccaga aaaacattcg ttacatattt gtgaattttt
     3361 ccaaaaaata acatttttat aaaacgacga aaaataaatc actaccagtc aacttctata
     3421 atattaccaa ata
//
LOCUS       DAZKKW010000162         3179 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.166, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000162 DAZKKW010000000
VERSION     DAZKKW010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3179
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.166"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..554
                     /locus_tag="ACG8PO_002783"
     CDS             <1..554
                     /locus_tag="ACG8PO_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HFQ0208037.1"
                     /translation="KKKYVLQVFHPVVTELENIEKQILALINSMEQQSQPIICILPNS
                     DAGSEIIRKMYQKYLKTDIIYITNLEPEEYLTILKECKFMIGNSSSGIIEAASFKIPV
                     INLGTRQNGREQSKNIINANLNENEIETALNKIQDPQFIKSLKNVKNIYGDGNSSRRI
                     TKTLNQILNDGEDIKWIQKQIAY"
     gene            527..1204
                     /locus_tag="ACG8PO_002784"
     CDS             527..1204
                     /locus_tag="ACG8PO_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFQ0208038.1"
                     /translation="MDSKTNSLLKYTSDPYIIIGAGGHAKVIIETLRLENKLIYGFFD
                     DKQNKMQDLKYLGKINELNSWADSKKKIYKFIVAIGDCVVRKKIIKKLNMPSEQYGIA
                     VHPSAIVAKEISIGYGTVIFANAVINPDAVIGEHAIINTGSIVEHDCRINNYVHLSPG
                     VCLAGGVHVGVGTQIGIGSQCIQMKKIGSWCMIGAGSTIVKDIPSHSLAYGNPAKIKK
                     EGINFEF"
     gene            1155..2336
                     /locus_tag="ACG8PO_002785"
     CDS             1155..2336
                     /locus_tag="ACG8PO_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFQ0208039.1"
                     /translation="MAIQLKLRKKELILNSRILLSSPHMGGTEEKYVAKAFKDNWIAP
                     LGENVDKFEESITKYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATV
                     NPILYQGAVPVFIDSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYER
                     ILAIANQFNVPVIEDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSD
                     KKYVEHSRFLATQARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIF
                     NRYKEAFSGIDRIEMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRA
                     LWKPLHLQPVFQGVKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIF
                     K"
     gene            2349..>3179
                     /locus_tag="ACG8PO_002786"
     CDS             2349..>3179
                     /locus_tag="ACG8PO_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HFQ0208040.1"
                     /translation="MNIGIIGLGSMGKRRLRLIQEHFNDILLCGVDTNEARRKEVEKE
                     FGIPTYNSLTEAKNLFEINSVFVCTSPITHEFIIKEALNLDCNIFTEINLLNEYYDEI
                     IEVAKKKDRLLYLSSTFLKRKEIQYIKSKVTHNKKISYQYHVGQYLPDWHPWEDYRNF
                     FVSNKKTNGCREIFAIELPWIIDTFGKVKSFTVMTQKLSNLDIDYSDSFTLIIEHETG
                     IVGTLSVDVVSRVAKRNLTIIGEKILIEWEGNPDSLFSWNEETSSMTDIQLYDEVMHD
                     N"
BASE COUNT         1186 a          408 c          608 g          977 t
ORIGIN      
        1 aaaagaaaaa atacgttttg caagtatttc atccagttgt aaccgaacta gaaaatatag
       61 aaaaacaaat tttagctttg atcaattcaa tggaacaaca atctcaacca attatttgca
      121 ttttacctaa tagtgatgct ggtagcgaaa ttatcagaaa aatgtaccag aaatatttaa
      181 aaacggatat tatttatatt acgaatttag agccggagga atacctgact atattaaaag
      241 agtgcaaatt tatgatcgga aattcttctt ctgggataat tgaagctgca tcattcaaaa
      301 taccagtaat aaatttagga accagacaaa atggacgaga acaatctaaa aatattataa
      361 atgcaaatct aaatgaaaat gaaattgaaa ctgctttgaa taaaatacaa gatcctcagt
      421 ttataaaaag cttgaaaaat gttaagaaca tctacggtga cggcaacagt tctagaagaa
      481 taacaaaaac attgaatcaa atattgaatg atggggaaga tattaagtgg attcaaaaac
      541 aaatagctta ttaaaatata ctagtgatcc atacattatt attggggctg gcggacacgc
      601 aaaagtcatt attgaaacct taagattaga aaataagtta atatatggat tttttgatga
      661 taagcaaaat aaaatgcaag atctgaaata tcttgggaaa ataaacgaat tgaatagctg
      721 ggcggatagc aaaaaaaaaa tctataaatt tattgtagct attggtgact gtgtagtaag
      781 aaaaaagatt ataaaaaaat tgaatatgcc aagtgagcaa tatggaatag ctgttcatcc
      841 aagtgctata gtggcaaaag aaatttcaat tggttatgga actgttattt ttgcgaatgc
      901 tgtaattaac ccagatgctg taattggaga gcatgctata ataaacacag gtagcattgt
      961 tgagcatgat tgtagaatta ataactatgt tcatttatct ccaggtgtat gcttagcggg
     1021 tggagtacat gtaggtgtag gaactcaaat aggaattggt tctcaatgta tccagatgaa
     1081 gaaaattgga tcttggtgta tgataggcgc agggagcact attgttaagg atattccctc
     1141 acattcctta gcttatggca atccagctaa aattaagaaa gaaggaatta attttgaatt
     1201 ctagaatttt attatcaagt cctcatatgg gaggaacaga agaaaaatat gttgctaagg
     1261 cttttaaaga taattggata gcgccattag gtgaaaatgt ggataaattt gaagaaagta
     1321 ttacaaaata tactggttct aagggcggag cttctgcgtt aagttcaggt acagctgcca
     1381 tacatcttgc cttaagactg cttaatgttg gaaaaaacga tattgtcttt gctccttcat
     1441 ttacttttat agctacagta aatcctattt tatatcaagg ggctgtacca gtgttcattg
     1501 actcagaacc taatacatgg aatatgagcc ctgttgcact agagagcgca ttagaagaag
     1561 ctgaaagaaa caaccaatta cctaaggcaa tcatcgtagt gaatttatat ggtcaaatgg
     1621 ctgattatga acgaatttta gctattgcaa accaatttaa tgttccagtg atagaagatg
     1681 cagcggaatc gttaggttct acttataagg gaatttcgtc aggcttattt gggacaattg
     1741 ggatctattc atttaacggt aataagatca tcacaacctc aggtggagga gctctagtgt
     1801 ctagtgataa aaaatatgtt gagcattcaa gatttttagc aacacaagca agagatcaag
     1861 caccgtatta tcaacatagc gaaataggtt ataattaccg tctaagcaat atttctgccg
     1921 gtatcggacg tggacaaatg gaagtattga atgatagagt agctaaacga agagaaattt
     1981 tcaatcgata taaggaagct ttttctggaa ttgatagaat tgaaatgatg ccagaagtgg
     2041 atggcatggt gccaaatcgt tggttgtcta ctttaacgat caatgattat aattctattg
     2101 atcaagtagt taacgtaata aataaactaa atgaaaaaaa cattgaaaca agagctttgt
     2161 ggaaaccact acatttgcaa ccagtatttc aaggggtaaa aatgtattac catgataata
     2221 atcttagtgt gtcagaaaag ttatttaata gcggcttatg cttaccttct ggaagtaata
     2281 tgacagatag tgaacaagaa tttgttattg atgagtttaa aaaaatattc aagtaggtgt
     2341 gatattttat gaacatagga attattggtt taggttccat ggggaaaaga agacttagac
     2401 tcattcaaga acactttaat gatattttac tatgtggtgt tgatactaat gaggcgagac
     2461 gaaaggaagt tgaaaaggaa tttggaatac caacttataa tagtctaaca gaggcaaaaa
     2521 atttgtttga aataaattca gtttttgttt gtacatcccc tataacccat gaatttatta
     2581 taaaagaggc tttaaattta gattgtaata tctttacgga aatcaattta ttaaatgaat
     2641 attatgatga aataatcgaa gtagctaaaa agaaagatag attactttat ctatcttcta
     2701 catttttgaa gagaaaagaa attcaatata ttaagtctaa agtgacgcat aataaaaaaa
     2761 tttcttatca ataccatgta ggacagtatt tacccgactg gcatccttgg gaagattatc
     2821 gcaatttttt tgtttcaaac aaaaaaacta atgggtgtag agaaatattc gctattgaat
     2881 taccttggat tatagataca tttgggaaag ttaaatcgtt tactgtaatg acccagaaat
     2941 tatccaatct tgatattgat tattcggaca gtttcactct tattattgag catgaaactg
     3001 gtatagttgg aactttaagt gtagatgttg tttcaagagt agctaaaaga aatttaacta
     3061 taattggtga aaagatttta atagaatggg aaggtaaccc ggattctcta ttttcatgga
     3121 atgaagaaac ttctagtatg acagatattc aattgtacga tgaggttatg catgataat
//
LOCUS       DAZKKW010000163         3153 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.167, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000163 DAZKKW010000000
VERSION     DAZKKW010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3153
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.167"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..742)
                     /locus_tag="ACG8PO_002787"
     CDS             complement(<1..742)
                     /locus_tag="ACG8PO_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0208041.1"
                     /translation="MERKFGIRDKIGYMFGDFGNDFTFIFASSFLMIFYTKVLGISGA
                     AVGTLFLVARFIDAVTDVTMGRIVDRSKATKQGKFKPWILRMSGPVALASFLMYQTSM
                     ISASMSVKVVYMYVTYILWGSIFYTSINIPYGSMASAITSITEERTMLSTFRNVGATL
                     ASLVIGVLTPLFIYTRDDSGAQIVRGGSTFTIVAGIFSICALICYLICYKLTTERVRI
                     DQSQYKVSLLKSLKGLVKNRALLSIIGAA"
     gene            complement(862..1509)
                     /locus_tag="ACG8PO_002788"
     CDS             complement(862..1509)
                     /locus_tag="ACG8PO_002788"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-keto-4-hydroxyglutarate
                     aldolase/2-keto-3-deoxy-6-phosphogluconate aldolase"
                     /protein_id="HFQ0208042.1"
                     /translation="MKKVEILSKLEAAGVIAVVRGKNKDEAIKAINSIIAGGIKGIEL
                     TFTVPNAEEIIKEIAKKYADQKDVIIGAGTVLDAFTARIAIMAGAEYIVSPSFDRQTA
                     EICNLYQIPYLPGCMTITEIKEALTAGVDIIKLFPGSAFGPSIVKAYQAPLPHVNIMP
                     TGGVSIENLEDWFEVGVVAVGVGGNLLASVEKGDFEEVSRVASKYIDKFYEIKNY"
     gene            complement(1593..3020)
                     /gene="uxaC"
                     /locus_tag="ACG8PO_002789"
     CDS             complement(1593..3020)
                     /gene="uxaC"
                     /locus_tag="ACG8PO_002789"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFQ0208043.1"
                     /translation="MFLDKNFLLENEIAKRLFHKFAKHQPIIDYHCHLDPAYLLENKQ
                     YKNLTQVWLNDKGAGDHYKWRLMRANGIEEELISGPGDDYEKFLAFVKTVEKAPGNPI
                     FEWSHLELQRYFDIDLTISEKNAPEIWRRANEKIQTDSYRPKELLEMMDVKAICTTDD
                     PTDDLWYHTELSKQDNSFKVLPTFRPDKVWSIKSSGFSNYILKLSQTAAIPIKDFNGL
                     KQALSQRVDHFHQIGCRLADHGLNTYFYEIADEKILDNLLIKALAGKTDFHLSEIAQF
                     VTGIQLHLMSEYTRYGWTMQMHLNALRNNSSILKEQIGVDVGGDSMGDQASLTQNVVA
                     LFDTAEKSGCLPKTILYSLNPTDWLPLATAMQSFQGGMIQKIQLGCAWWFNDTAEGMR
                     QQLTTMAQQSLLSNFTGMLTDSRSFLSYPRHEYFRRVLCQLLSEWVKAGRIPDDEMYL
                     SQIVENICYRNAYNYFNFFEIGSRK"
BASE COUNT         1003 a          661 c          420 g         1069 t
ORIGIN      
        1 tagccgcacc aattatagaa agtaatgccc tatttttaac taaccctttt aaagatttta
       61 acaatgaaac tttgtattga gattggtcaa ttcttactcg ttcagtagtt agtttataac
      121 atattaaata acaaataagt gcacatatcg aaaatattcc cgcaacaatt gtaaaagttg
      181 atccaccacg aactatttga gcgcctgaat catctcttgt ataaataaat aatggtgtta
      241 atacaccaat gaccaaactt gctaaagtag ctccaacatt tcggaatgtc gatagcattg
      301 ttctttcttc tgtaatagaa gtaatagctg atgccattga tccataagga atatttatag
      361 atgtgtaaaa aatactcccc cataatatat acgtaacata catgtatact accttaacgc
      421 tcatagaagc tgaaatcata gaagtttgat acattaaaaa acttgctaaa gcgactggac
      481 cactcattct taaaatccac ggtttaaact taccctgctt agttgctttc gatctatcaa
      541 caatacgccc catagttaca tcagtaactg catcgataaa tcttgctact aaaaataatg
      601 ttccaacggc agctccgctt atccccaata cttttgtata aaagatcatt aaaaaagaac
      661 tagcaaaaat gaaagtaaaa tcgtttccaa aatctccaaa catatatcct attttgtctc
      721 ttataccaaa ttttctttcc atacaattct ctcccttctg ttgttaagcg ttttcattct
      781 gatgaaagta cgtactatac tctgaagcgt aaagagtcag tatttatttt ttacatttca
      841 gagtattatt tgttttttct cctaataatt ctttatctca taaaacttat ctatatattt
      901 actagctact cgacttactt cttcaaaatc acctttttca acagaagcca ataaattccc
      961 acctacacca actgcaacaa ctcctacttc aaaccaatct tctaagttct ctatacttac
     1021 tccgccagta ggcattatat ttacgtgtgg taatggagct tgataggctt ttacaatgga
     1081 tggaccaaaa gcagaacctg ggaatagttt aatgatatct acgccagcag taagtgcctc
     1141 ctttatctca gtaattgtca tacaacctgg taaataagga atttgatata aattacaaat
     1201 ctcagctgtt tgtcgatcaa aagatggact aacgatatat tctgccccag ccataattgc
     1261 gatacgagca gtaaacgcat ctaaaaccgt accggctccg attattacat ctttttgatc
     1321 agcatatttt ttagcaattt ctttgattat ttcttcagca ttaggaactg taaaagttag
     1381 ttcaatccct ttaattccac ccgcaataat tgaatttatt gctttaatgg cctcatcctt
     1441 atttttccca cgtacaaccg caatgactcc agccgcttct aacttactta aaatttctac
     1501 ttttttcata tctatctcct tcctttatgg atacatactt tgtctagctg tactaactta
     1561 caaagaataa atttaagctt ttatttcaag tattattttc tacttccgat ttcaaaaaaa
     1621 ttaaaataat tatacgcatt acgataacaa atattctcca ctatctgact aagatacatt
     1681 tcatcatctg gaattcggcc tgctttaacc cactcgctta gtaattgaca taatactcga
     1741 cgaaaatact catggcgagg atagcttaga aaacttcgag aatcagtcaa catacctgta
     1801 aaattcgaca acaaactttg ttgtgccatc gtagtcaact gttgacgcat tccttcagct
     1861 gtatcattga accaccaagc acatcccaat tggatttttt gaatcatacc cccttggaag
     1921 ctttgcattg ctgttgctag tggaagccaa tcagttggat ttaatgaata taaaattgtt
     1981 tttggtaagc acccactctt ttcagcggta tcgaacaatg ctacaacatt ttgtgtcaaa
     2041 ctagcttgat cccccatcga atcaccaccg acatctactc caatttgttc ctttaatata
     2101 cttgaattat ttcttaaagc atttaaatgc atttgcatcg tccaaccata tcttgtatac
     2161 tcagacatca aatgtaattg aatacctgta acaaattgtg caatttctga taaatggaaa
     2221 tccgtttttc ctgctaaagc ttttattaac agattgtcga gtattttttc atctgctatt
     2281 tcataaaaat aagtattcaa accgtgatct gctaatcgac agccaatttg atgaaaatga
     2341 tccactcgtt ggctcaatgc ttgcttcaac ccattaaagt ctttgattgg tatagccgct
     2401 gtttgactaa gttttaaaat atagttcgaa aagcctgatg atttgatact ccagacctta
     2461 tctggacgga aagtaggtaa taccttgaaa gaattatcct gtttagaaag ctcagtatga
     2521 taccacaagt catctgtagg atcatccgtt gtacagatgg ctttcacatc catcatctcc
     2581 aacaattctt tcgggcgata ggaatccgtt tgaatttttt cgttagcacg tcgccaaatt
     2641 tctggtgcat tcttctcaga aattgtcaaa tcaatatcaa agtaacgttg taattccaaa
     2701 tgagaccatt caaaaatagg attacctggc gccttctcca ctgtttttac aaaagctaaa
     2761 aatttttcat aatcatcacc tggaccactg atcaactctt cttctatacc attagctcgc
     2821 atcaatcgcc atttataatg atctccagct cctttatcat ttaaccatac ctgagtcaaa
     2881 ttcttgtatt gcttattttc tagcaaatat gctgggtcta aatgacaatg atagtcgata
     2941 attggttggt gttttgcaaa tttatgaaaa agtcttttag ctatttcatt ctctaataaa
     3001 aagttcttat ctaaaaacat atttaaacac tcctttcctt ccgttagttt ttagaagaga
     3061 aaatttttcc tctaaataaa ctcccttata taattattta ctcgccccct tttcaagcgc
     3121 ttcccaaata ccatttaaat aaactgcacc tag
//
LOCUS       DAZKKW010000164         3127 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.168, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000164 DAZKKW010000000
VERSION     DAZKKW010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3127
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.168"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            465..611
                     /locus_tag="ACG8PO_002790"
                     /pseudo
     CDS             465..611
                     /locus_tag="ACG8PO_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332517.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamate
                     endopeptidase"
     gene            complement(699..992)
                     /locus_tag="ACG8PO_002791"
     CDS             complement(699..992)
                     /locus_tag="ACG8PO_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HFQ0208044.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
     gene            1029..1214
                     /locus_tag="ACG8PO_002792"
     CDS             1029..1214
                     /locus_tag="ACG8PO_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0208045.1"
                     /translation="MLEYSGFFIKAVTLKKDIIIVLFFLLLLIGRFILIHYSPLPIIK
                     EKTGSGCSNYMLRKKNP"
     gene            complement(1395..2573)
                     /locus_tag="ACG8PO_002793"
     CDS             complement(1395..2573)
                     /locus_tag="ACG8PO_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0208046.1"
                     /translation="MENMSRFKKHITATGKITWYFQAFRTTRRGFNSKREAQLAYLEM
                     EKQHRNKQKRLASNDKFSVVSQKWFEYYRSLNEQKVATYDKREEQVKILNRWIGDKKL
                     SSLSSEELQKFLFILKEKGIDGVNVGYAKNSLQSLVQVLNMVFNYCKKKNLINENPMK
                     FVKMPKYQLSVKDLKESINSLEDKFLTVDELRAFLNYGIVYEELPMSVLFHVLFYTGC
                     RVSEALALQPQDIDFERNEILFYKQTAVKGKSKDFRIETTKTVSSARRVPVTLLVMEK
                     LQELIDVLNKTKRNSNFVVDETYLFVYLDPGKRGVPYRREYVNDHVKRCVERYGINKD
                     FHTHLARHTMASLVAEYCSWDVLKDRLGHTDSTTSKIYRHLTSTEKLKPLTAFNSLEG
                     "
     gene            complement(2676..2987)
                     /locus_tag="ACG8PO_002794"
     CDS             complement(2676..2987)
                     /locus_tag="ACG8PO_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0208047.1"
                     /translation="MSKNNNKSNSSELEKYLFHPEKIEDSNNKMKRINQLPLVLTNKD
                     LKEQFQISDSTLNRLIKLGDFPSCWFGIRGHYLRDDILEWVKKGNEEYFCDQFHLLRL
                     L"
BASE COUNT         1005 a          560 c          406 g         1156 t
ORIGIN      
        1 agattttatt tttaaataaa aaatactctt tttttacgat tattgaaaaa aaaagataaa
       61 acttgattaa ttgaagtatt gcttaaattt ggaagctatg agggaagaat aaaagagtta
      121 ggacgggatt atctactatt tttctttgtt ttaaatattt gttatctttg aacataaaag
      181 agaaatgtgt cgactgcagt gttgtgtccc tcatcacgaa taaacggtgt tcaactagct
      241 gatcttgtat tcacttaaaa aatggtaaag tacgagttgc tattccccat tttcaatttt
      301 ctaacgctca ggacaaaata ctgttttcac aacctcattg gaataaaagc atatatattg
      361 tttcaagaac gaaatcttgt tatcttttta aagggcatct gtccaaaatc taattttagg
      421 aggtacgttt tggtatttat tcgtacaaat aaaagagaat tatctttatt catgtaacgc
      481 atccaattga ttttttcatg tacgctcctg gaacgatttt actgacgatt gcttatttaa
      541 ctgcgaatcg ctcattagct tttatcatag ctatccatat tttgaataac gttcttggct
      601 ttgtattata gatacagatg acaaaaaaag ttcaagtatg cggtgtagaa ccaatatcgc
      661 atacttgaac ttttttcaat gagtataaat taaaacttct aattttcaat tcccatcatc
      721 tgatatgccc gaccgtctga acttcttttg aaaaagccaa atgtgattag ataacgtctt
      781 tgttcgacat aatcgccact agaaaatacg ttcagcagta tcgaattaac ctctgtttct
      841 gtataagtca ctccatggtc aaagcgtttc aagacttctt tcaataggat gtatttcttt
      901 tttgttgaac ttggtacttg ttttactttt ccatcattca tgaatgtgtc taaaactttc
      961 atcttttctt gctccgtata agtataaacc atcttctttc tccttttgat tctaagaata
     1021 ctccatttat gttagaatat tctggatttt ttattaaagc ggttactctt aagaaagata
     1081 ttatcatagt gctctttttt ttactactac ttataggtag atttattcta atccattatt
     1141 ctcccctacc aataattaaa gaaaaaactg gatcagggtg ttcaaattac atgctaagaa
     1201 aaaagaaccc ttgaatacca agggttcttt ctcagtctga taacaatgat taacgacgga
     1261 tttctttgat acttgtgaaa agtctttaca gtattgatat aaaagaattt ttattgcctc
     1321 tgactacact ttgactaacc tattaaaaaa ttgtttttca agaatcctgt aataccagta
     1381 ctttcaccat ctttttaacc ttctaaagaa ttaaaagcag tcagaggctt taacttttca
     1441 gttgatgtta aatggcgata tatttttgag gttgtactgt ctgtatgacc taaacgatcc
     1501 tttaaaacat cccaactgca atactctgcc actaaactag ccatagtatg acgagccaaa
     1561 tgggtgtgaa aatctttgtt aattccatat cgctctacac aacgtttaac atgatcgttt
     1621 acatattcac gacgataagg aactccacgc tttccaggat cgagataaac aaataaataa
     1681 gtttcatcta caacaaaatt actatttctt tttgttttat ttaatacatc aattaattct
     1741 tgtaatttct ccatcactaa tagtgtgacg ggtactcttc ttgcagaact aacagttttt
     1801 gtagtttcta ttcgaaaatc tttacttttt ccttttactg ctgtctgttt ataaaacaaa
     1861 atttcatttc gttcaaaatc tatatcttga ggctgtaaag caagcgcttc actcacacga
     1921 cacccagtat aaaacaaaac atgaaaaaga acagacatgg gtaactcttc ataaactatc
     1981 ccatagttta aaaaagctcg taattcatcc actgttaaaa acttatcttc taaactgttg
     2041 atagactctt ttaaatcttt aacagataac tgatatttag gcattttaac aaacttcatt
     2101 ggattctcat ttatcaaatt ctttttctta caataattaa aaaccatatt tagaacctga
     2161 acaagagatt gtaaactatt tttagcataa ccaacattaa cgccatcaat tcccttttct
     2221 tttaaaatga aaaggaattt ttgtagttct tctgatgata aactacttaa ttttttatca
     2281 ccaatccatc gattaagtat tttcacctgt tcttctcgtt tatcatacgt tgccaccttt
     2341 tgttcgttta gtgatcgata atattcaaac cacttttgac taacaacact aaatttatca
     2401 ttactagcaa gccttttttg cttatttctg tgttgttttt ccatttcaag ataagcaagc
     2461 tgagcttcac gtttagaatt aaaacctcgt ctcgtagttc gaaaagcctg aaagtaccaa
     2521 gtaatttttc ccgttgcagt aatatgtttt ttaaaacgac tcatattttc catttattct
     2581 tacctccaat tacgaaggaa cccaacaatc ggaagtgctt gaaaatcttt ctatctatga
     2641 aataattata caacatgaag acgaaaagat aaattctaca ataatcgtaa taaatgaaac
     2701 tgatcgcaaa aatattcttc atttcctttt tttacccatt ctaaaatgtc atctctaagg
     2761 taatgtcctc taatgccaaa ccagcagctt ggaaaatcac ctaattttat aagacggttt
     2821 aaagtactat cacttatttg aaattgttct tttaaatctt tattcgttaa aactaatggt
     2881 agttgattta tcctcttcat tttattatta gaatcctcta tcttttctgg atgaaacaaa
     2941 tatttttcta actctgatga attactttta ttgttattct ttgacatgat tgtctcttcc
     3001 tttctattaa caataattaa tctatcagcc acttttgtca aaagtacata ggaatttcac
     3061 ctctccccaa gtttacggcg agccttacgg aagtatcatt ataccaactt caaatcatcg
     3121 cttttta
//
LOCUS       DAZKKW010000165         3063 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.169, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000165 DAZKKW010000000
VERSION     DAZKKW010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3063
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.169"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            580..1539
                     /locus_tag="ACG8PO_002795"
     CDS             580..1539
                     /locus_tag="ACG8PO_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFQ0208048.1"
                     /translation="MTYTHLTPTELVMIEAYFNQSHSVSKVAHLVQRSRQTIYNVYRF
                     LKSGGSAISYYEQYKKNKRNCGRRPIVLPDGQQEYIQKKVAQGWTPDVLIGRAEFPIA
                     CSVRTLYRMFKDKTFDPHDLPMKGKRKPNGHKEKRGKQAFRRSIQDRTTDYIQFNHEF
                     GHLEGDTIVGGKHKSAVITLVERLSKVIITLKPAGRQAIDIENRLNQWFQSVPKHLFK
                     SITFDCGKEFSNWKKISNTNDISIYFADPGTPSQRGLNEHSNGLLRKDGLYKAMDFNK
                     VSETFIQSVASKRNHIPRKSLHYRTPLEVFLSYVGKDDLSSLI"
     gene            complement(1526..2131)
                     /locus_tag="ACG8PO_002796"
     CDS             complement(1526..2131)
                     /locus_tag="ACG8PO_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0208049.1"
                     /translation="MINSAYFHIKYLKKYEYEEVVLDYGLNRQSFLIYAINNGNLPSN
                     KKNIIIETYAYLEGGSKKLIKSCNIEYQLSVGEIKCVFEEDLELDLKKIFASIHDYIG
                     LIYKIIEDDKQTELNMCYFDREQDFFYYGGMGGGSIVPKINLVELDVNNYRELYVFNS
                     LDFEIPMKQMKNIDIHILPSESCQIEYSIEYFYKVSFVKLS"
     gene            complement(2371..>3063)
                     /locus_tag="ACG8PO_002797"
     CDS             complement(2371..>3063)
                     /locus_tag="ACG8PO_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HFQ0208050.1"
                     /translation="ATISRHSSGKYYISLLCKEEVRELPKSNSAVGIDLGIIDFAILS
                     TGQKFDNNKFTSKMEKKLKREQRKLSRRALLAKQKGINLFEARNYQTQKRKVARLHEK
                     VMNQRTDFLNKLSTEIIKNHDIICIEDLNTKGMLRNHKLAKSISDVSWSSFVSKLQYK
                     ADWYGRKIIKVDKWFPSSQICSNCGHKDGKKSLEIRKWTCPICHTHHDRDINASINIL
                     TEGLRLHSMGLA"
BASE COUNT          997 a          509 c          478 g         1079 t
ORIGIN      
        1 acaataaatg aagacttttt tattgttaat tgtttatttt tctattatta gatatgaaaa
       61 tcgcatttag ataaaataga aaaatatgaa tatattttaa atattaagat gagagtcatt
      121 aacttatgat agtagtcaat ttgcttttca ctaattacaa aatatgtgat ataggaagtt
      181 tagttattgt tctcataatg ccatcctgtt tttagattat ctagtaattt ttttgttatt
      241 taaaattagg agcagtgcca attgttttaa ttttaaaggc tttttttata tcttctaaat
      301 tagaagatat ttgtatatca atatggaata tattaccttt tttgtatttt tttatgttat
      361 ctttagctaa aacatcaaat acatgtcctt gaaattgtat cgaggcagct ggatttagtt
      421 taaattgagg aaactttacc acagctgatt caaaacactt gtcacatttt tttatttttt
      481 taccttcgcc gattgtaaaa ttaagctaga caactaaaaa agtcatttgt gctacactca
      541 aaatagttcc gtcgaaagaa ttataaggag tgagtacaaa tgacctatac ccatcttaca
      601 ccaactgaac tcgtaatgat agaagcttat ttcaatcaat cacactctgt ttcaaaagta
      661 gcccatctgg tacaacgttc tcgacaaacc atttataatg tctatcgttt tcttaaatct
      721 ggtggaagcg caataagcta ctatgaacaa tataagaaaa acaaacgtaa ctgcggaaga
      781 cgtccgattg tgttaccaga tgggcaacaa gaatacattc aaaaaaaggt ggctcaaggg
      841 tggactccag atgtccttat tggtcgagca gagtttccta ttgcttgttc ggtacgtaca
      901 ctatatcgga tgtttaaaga taaaaccttt gaccctcacg atttaccaat gaaagggaaa
      961 agaaaaccaa acggacataa agaaaaaaga ggaaaacaag cttttcgccg ttcgattcag
     1021 gatagaacaa ctgattatat acagttcaat catgagtttg gtcacctaga aggtgacacc
     1081 attgtcggtg gaaaacataa aagtgcggtg atcacgttag ttgagcgatt atctaaagtg
     1141 atcatcacct tgaaacccgc tggtaggcaa gctatagata ttgagaatag actgaatcaa
     1201 tggtttcaat ctgtaccgaa acacttgttt aaatccatca catttgattg tggaaaagag
     1261 ttctcgaatt ggaaaaagat tagtaatacc aatgatattt cgatttattt cgctgatcca
     1321 ggaacacctt ctcaacgagg actaaatgaa cattcgaatg gcttattacg taaagatggc
     1381 ttatataaag ctatggattt taataaggta tccgaaacct ttatccaatc ggtggcatca
     1441 aaacgaaatc atattcctag aaaatcatta cactatcgca caccattgga agtatttttg
     1501 agttacgtag gtaaagatga tttgtctagc ttaatttgac aaacgaaact ttgtaaaaat
     1561 attcgattga atattctatt tggcacgact cacttggcag tatatgtatg tcaatatttt
     1621 tcatctgttt cataggtatt tcaaaatcta aactattaaa aacatataat tccctgtaat
     1681 tatttacatc taactctact aaatttatct ttggaacaat agatcctcca cccattccac
     1741 cataataaaa aaaatcttgt tctctatcaa aatagcacat attaagctca gtttgtttat
     1801 catcctcaat aattttataa atcaaaccta tataatcatg aatgctggca aaaatttttt
     1861 ttaagtcaag ttctaaatct tcttcgaaaa cacatttaat ttctcctact gataattgat
     1921 actctatatt acatgactta attagttttt tagatccccc ctccagatag gcatatgttt
     1981 caataattat gttcttttta ttgcttggca aatttccgtt atttattgca taaattaaaa
     2041 atgactgtct atttagccca taatctaata caacttcttc atactcatat ttctttaaat
     2101 atttaatatg aaaatatgct gaattaatca aaactcgagt gttactgttg ttatttattg
     2161 ttgttctatt gtgtataaaa ttgtataggt tgtcatattt tcttggttta gtgaactact
     2221 caccacttag ctaaacctaa cggtttctta cgtttgaagt gggagcttct tgggaagaga
     2281 gcatacttga tagcgtatta ctttactaac agaggtgcct cttatttacc aagctatccc
     2341 cgtagttcct acggttcttg tttatttgat ctaagccaat cccatagagt gtagccttag
     2401 accttcggtc aggatattga tactagcgtt gatatctcgg tcatgatgag tatgacaaat
     2461 agggcaagtc cattttcgaa tttcgagaga tttcttgccg tctttatgtc cacaattcga
     2521 acagatttgg ctagatggaa accacttatc tactttgatg attttccgac cgtaccagtc
     2581 agccttgtat tgtaatttag acacaaaact agaccaagaa acatccgaaa tactttttgc
     2641 tagtttatga ttacgcaaca tgccttttgt gtttaagtct tcaatacaga tgatatcgtg
     2701 atttttgata atttctgtac tcaacttatt cagaaaatca gtacgttgat tcattacttt
     2761 ttcatgcaat cgtgctactt tacgtttttg tgtttgataa tttctagctt caaacagatt
     2821 gatacctttt tgtttagcta ataaggcacg tcttgacaac ttacgttgtt cacgtttgag
     2881 tttcttttcc attttggacg taaatttatt attatcaaat ttttgaccgg tagaaagaat
     2941 tgcaaagtct ataataccta ggtcaatacc aacagcagaa ttagatttag gtagttcgcg
     3001 aacttcttct ttgcataaca aagaaatata atatttaccg ctagaatgac gtgatattgt
     3061 agc
//
LOCUS       DAZKKW010000166         3058 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.170, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000166 DAZKKW010000000
VERSION     DAZKKW010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3058
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.170"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..227
                     /locus_tag="ACG8PO_002798"
     CDS             <1..227
                     /locus_tag="ACG8PO_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0208051.1"
                     /translation="KTTEFDILHALMSTPNKVFSRSDLIEKVKGIEFDGLDRSIDSHI
                     KNLRHKIENDAKSPMYILTVHGTGYRFGGH"
     gene            227..1620
                     /locus_tag="ACG8PO_002799"
                     /pseudo
     CDS             227..1620
                     /locus_tag="ACG8PO_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294791.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
     gene            1635..1841
                     /locus_tag="ACG8PO_002800"
     CDS             1635..1841
                     /locus_tag="ACG8PO_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0208052.1"
                     /translation="MFQKQIKIAKLVCECWAKKIFFQLISIDSVEKVHLDILSNSVVI
                     FSKKDLSIATIKKEVFGLQIYEMT"
     gene            1862..2458
                     /locus_tag="ACG8PO_002801"
     CDS             1862..2458
                     /locus_tag="ACG8PO_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhK family protein"
                     /protein_id="HFQ0208053.1"
                     /translation="MKTVKKMTKLVFASLGIIVFLGACSNQSESNNSNSTNEESTSTA
                     SSEMNSMEGMNHEGMVPSSMKDAANPKFPVGSNVILLGDHMEGMKGANAQVVGAFDTT
                     MYEVSYEPKTGGPMVNNHRWVVQEELKDTETVANEGDTVILNADHMDGMMGAEAKVDK
                     SITGTVYVVNYTPTDGQEEVKNHMWVTEDEMEYDENNE"
BASE COUNT         1117 a          412 c          594 g          935 t
ORIGIN      
        1 ttaaaacaac tgaatttgat attctacacg ctctaatgtc cacacctaat aaagtctttt
       61 ctcgtagtga tttgatagag aaagttaagg gaatagaatt tgatggacta gataggagca
      121 tcgattctca tataaaaaac ttaagacata aaattgagaa tgatgctaaa tcacctatgt
      181 atattttgac agtccatggt acaggataca gatttggtgg ccactaatga gaaaaagaac
      241 gataaaaact caattagctg tttccttttt agcaatcgca acattaataa taggatcgat
      301 tagtttagtc gcattaagtt tgatgaacaa tcattttagt aaatacgtgg aagagcggca
      361 agaagatttg ttgaatcagt atgtatatac aattgattta ttgtggttaa atagtgggga
      421 aacatggaat agtgaagaac ttgcagcact atctgagaaa gtattagaga ataatattta
      481 tttttctata gaagacgaac aaggcaacat ggtttgggaa cttactggaa aagatttgaa
      541 gtcagcacag gagaaactga aaaaaatgca ttgaaagttt ctgagaaaaa cagtgtgaaa
      601 ctagacgaaa caatagaagt gaaaaagaag ttaattaatg atggaaacga gtttggaaaa
      661 gtaacgtttt attatttcgg accttttgct tatacagagc acgatgcctt gtttatctcc
      721 agtatgaaac aaagtttaat gtatgttgca attgcagctc ttcttgtatc attcatactt
      781 gcttcgtgga tctctgctag attaggattg ccactaaagc atgtaagcga ttttacacat
      841 aaattaactc gtggggaata tgctgataaa atcccacaag aaacttcaat tattgaaata
      901 aattccttaa ttgattccct aaatgattta agtaatcagt tggagaaaca gcatgggctt
      961 cgaaaaagat taactaccga tatttcacat gaattaagaa ctccattagc tacgcttaaa
     1021 ggcaatgtgg aaggaatgat tgacggagtc tggaaaataa ctcccgagcg cctacaatct
     1081 tgttatgacg aaatagatcg tctaactcgt ttaattggaa atatagaaat aatcaacaaa
     1141 attgaagcaa aatatgatca tttaaataaa actgagttta acatatataa gttgattgaa
     1201 tccgttattg aaaatttcgc cagcaaaatt gagtcaaaaa accttcatgt tgaaatccaa
     1261 ggggataaca tcaatatatc tgcagataaa gataaaatga accaagttgt tatcaatctt
     1321 ttgaataatg ctattaaatt tacgcaaaag gagggaacca ttaagttttc tataagtaaa
     1381 aataaagacc atgttctttt aattgttgaa gacaacggca tcggtataga aaaagatcag
     1441 caacttcata tttttgatag attttatatg gctgacccct ccagaagtag agctttaggg
     1501 gggcaaggta tcggattagc tattgtaaag agcgttattg aagcgcataa aggtagtata
     1561 actgtaaaaa gtaaattagg tctaggaact aaatttgtta taaagttacc atttcaataa
     1621 aggaggagag ttatttgttt caaaaacaga taaaaattgc taaactagta tgtgaatgtt
     1681 gggcaaaaaa aatatttttt caactgattt cgatagatag tgtcgaaaaa gttcatttag
     1741 atatcttatc aaattctgta gtaatctttt ctaagaagga cttatcaata gctacaatta
     1801 aaaaagaagt ttttggatta caaatttatg agatgaccta aatgtgatta attaaaggag
     1861 gatgaagaca gtgaaaaaga tgacaaaatt agtatttgca agtcttggaa ttattgtatt
     1921 tttaggagcg tgtagcaatc aaagtgaatc gaataattct aatagcacaa acgaggaatc
     1981 gacttctaca gcgtctagtg aaatgaatag tatggaggga atgaatcacg aaggtatggt
     2041 tccttcttca atgaaagatg ctgcgaaccc taaattcccg gtgggaagta atgttatact
     2101 gctaggcgat catatggaag ggatgaaagg tgcaaatgct caagttgtag gggcctttga
     2161 tactacaatg tatgaagtaa gctatgagcc aaaaactggg ggaccaatgg ttaacaatca
     2221 tcgatgggta gtacaagaag agttaaaaga tactgagact gtagcaaatg agggcgatac
     2281 tgttatcttg aatgccgatc acatggatgg gatgatggga gctgaagcaa aagtagataa
     2341 atctattaca ggaactgttt acgttgttaa ttacactcca accgatggtc aagaagaagt
     2401 taagaatcat atgtgggtaa ctgaagatga gatggaatat gacgaaaata atgagtaatt
     2461 ttatatacta aaccaacaag ctggtacatg gtcttcgtat tctcaacatg aggaatatga
     2521 gctctttgtt aataaggact gatgagttgt gcaaaatcaa atctgggtca gaacgaacct
     2581 cattctggct cagatttttg gctcttcgac atttagtgct ggtagagaga caactgaaaa
     2641 tttagtcata accagttagg cagagtgctt aactggtttt tgttcgcatt tattattctt
     2701 ttgatattgg ctgcttgccc ttcaatttta tttggtttct taatcaactc ttcatataac
     2761 cccaaatcat atattaatga ggtgcctcct attcctataa atctatctct tctttaatag
     2821 tatgagaaaa gtctactctt gtagatttaa attgtcaaca aaaaatttac agatgtaaat
     2881 gataagttga atagagaaag ataaattatt tttttggtat tcatataagg ttaattatgt
     2941 attatcacaa aatgattgtt gtcgaataac taaaagtgtc aaaacaatga tgaaatcatt
     3001 tagttcttat aaattagaca agagaaaata aatattccat atctacttta ttggatcc
//
LOCUS       DAZKKW010000167         3043 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.171, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000167 DAZKKW010000000
VERSION     DAZKKW010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3043
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.171"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(308..670)
                     /locus_tag="ACG8PO_002802"
     CDS             complement(308..670)
                     /locus_tag="ACG8PO_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0208054.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(744..1124)
                     /locus_tag="ACG8PO_002803"
     CDS             complement(744..1124)
                     /locus_tag="ACG8PO_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvfD/Ygs/GSP13 family RNA-binding
                     post-transcriptional regulator"
                     /protein_id="HFQ0208055.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1206..2381
                     /locus_tag="ACG8PO_002804"
     CDS             1206..2381
                     /locus_tag="ACG8PO_002804"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HFQ0208056.1"
                     /translation="MIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADMDFLSPG
                     GVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVLTSLAAA
                     VQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDMEKKIEEN
                     NVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHTFTSMLT
                     IDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEINTFGLI
                     GTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLDFTAYIS
                     DDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYALETID"
     gene            complement(2457..3041)
                     /locus_tag="ACG8PO_002805"
     CDS             complement(2457..3041)
                     /locus_tag="ACG8PO_002805"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFQ0208057.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGVGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
BASE COUNT          952 a          579 c          500 g         1012 t
ORIGIN      
        1 ttagtaacat gtcaataact attttttaaa ttttttagaa atgtgatata acatccactt
       61 cagaatcaac gctgttaaat ttatcatgta tttcttcctt ggtcaactat aaattaagca
      121 ttactttgtg aaatgatttt tcgatcaatt attattattc gttatacaac cgttttcaac
      181 atattttgaa aataaaatgc actaaaaaac tatctttagc agaacatttc attgtatttt
      241 ttggaccaaa caataaaaaa agaagtttga aaaaaattca aacttctttg ttcgcttaat
      301 ctgtatttta gaagaaaggc actccctcta cttcgtaagt tccaagaaca gagcgcattt
      361 gacctggttc ttcccaaata tatccttcgc ctaattcttc tctttgattc ttttgttcag
      421 tttcaaagac aaacaacgga acattttcct ctttaatatg tgtatttgtc aattccatca
      481 aactaatctt gctaacatcg atatgaacta tttcttttaa gaaattaagt atattcgcta
      541 aaccagtctt tccttcagaa agttccgcta acgcaaattc caacttgtca tcaattgaat
      601 ggaccaaaaa ttttttagta ccatcctcca aatgcaacat aattgtacca gcaactcttt
      661 tgcttgccaa aatttccacc tcgatcaact tgttctatgg cttacataaa aattctagca
      721 cattttcaga aaaaatacac tttttatttc ttattttctt tccgttctag ttcagataat
      781 gcttcttcta tccaaatagg taactgctcc gcaatagtgg taaatccgat cttacgattt
      841 ttattcgtga aatacctttt tctgcgatat ggaacagctg gatttgtttt ttctaacgtt
      901 cttaaagata aactgatttt tttggaatat tcatcaatgt cgatgatctg aactgttact
      961 ggctgtccga cttttaacaa tgaatggata tttttcgtat aaccggattg tacttctgaa
     1021 acatgaataa gtccttgcgt ctcgttatca agagaaacga atgcgccata aggctgtatc
     1081 cctgtcactt ttccttcaat tatttgtcca attttataag tcatctactt ccctctcttc
     1141 gcatttatca ttatactgta aatagctcaa aaaaagaagc gacgaaaatg actggaggtc
     1201 ttaagatgat cgaatttgat aaacagattt caagaaaaga tacagaaagt gtaaaatggg
     1261 acgcaattgc tgagacatat caaatggatg acttacttcc tttgtgggta gcagatatgg
     1321 acttcttgtc tcccggtggg gtagctaaag cgtttagtga atacatcaaa catggaatct
     1381 ttggttatgc cactctgtct gataaactat atcaggctat cattcattgg gaacgccaac
     1441 accatgctgt ggagttgacg aaagaaaata tcgtttttac aagtggtgtg ttaaccagtt
     1501 tagctgcagc agtacaaact tttacaaaac caggcgattc tattttgatt catgacccag
     1561 tttatcctcc attttcctct attatagaaa caaatcaacg tcacatcgtt cgaagctctt
     1621 tgctagaaaa aaataatcat ttcatcatgg acttgtctga catggagaaa aaaatcgaag
     1681 aaaacaatgt aaaagcaatg attttatgca atcctcacaa tcctggaggg cgtgtttgga
     1741 gcaaagaaga attgaaacag ttaagcgaat tgtgtttaaa acatcaagtg attctgttta
     1801 gtgacgagat ccatcaagat ttaactttat tcgatcatac atttacttcc atgctaacga
     1861 ttgatcctaa tttagatgat ctactagttg cttttacttc cgcaaccaag actttcaatc
     1921 tagctgcaat caaaaattca atggtattta tcaaaaatcc agagatgaaa gctgattttg
     1981 aaaaacattt agtgatgaac caacagcacg aaatcaacac ttttggactg attggaaccc
     2041 aagcggctta tgagacgggt ggagaatggt taagccaact gattccttat ctagaaaaaa
     2101 atgttcaaac agttaatcag ttttttgaac agtatcttcc gaaagtgaga ataatgaacc
     2161 ctgaaggaac ttacttaatg tggttagatt ttaccgccta tatttcagac gaccaagaat
     2221 tggagaacac tcttgttcgg aaaggaaaag tcgtattaaa tcctggcatc acgtttggtc
     2281 cttccggtca tgggcatatg cgcttgaata ttgcttgtcc agaagaaact ttactggaag
     2341 gacttaatcg aatcaagtat gctttagaga caatcgatta aatatttatt caaaataaaa
     2401 ggacgagacc ttactaggtt tcgtcctttt tcataattac tccgtaccat accaaactat
     2461 tctgaaattt caattgtttc aatcactaca tcatgtacag gtcggtcttg tggaccacgt
     2521 tgtacatttg cgatttcatc tactgtgtcc atcccttcta gtacatgtcc aaagactgta
     2581 tgtcggaaat ctagccaagg agttccgaca cctttatacg cttcaatgat ttctgatggg
     2641 aatccagcac cttctagttg tccaagcata ttagctggca catttgtgtt atttacaata
     2701 aagaattggc ttccgtttgt gtttggacct gaatttgcca tagataaagc tccgcgaagg
     2761 ttgaacagtt cacgactgaa ttcatcttca aaactttcgc cataaatact ttcgccaccc
     2821 attcctgttc cagttggatc gccgccttgg atcatgaaat caggaataac gcggtgaaaa
     2881 atcacgccat cataataacc ttttttggaa agttcaatga aattttgaac cgtcttcggt
     2941 gctagttcag ggaataattg aacagtaatg tcaccacggt ttgttttgat aacagctttt
     3001 ggtccttttt cactttttaa atctagttgt gggaatgcca tat
//
LOCUS       DAZKKW010000168         3034 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.172, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000168 DAZKKW010000000
VERSION     DAZKKW010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3034
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.172"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(251..865)
                     /locus_tag="ACG8PO_002806"
     CDS             complement(251..865)
                     /locus_tag="ACG8PO_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFQ0208058.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            complement(1471..1848)
                     /locus_tag="ACG8PO_002807"
     CDS             complement(1471..1848)
                     /locus_tag="ACG8PO_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0208059.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(2148..3029)
                     /gene="galU"
                     /locus_tag="ACG8PO_002808"
     CDS             complement(2148..3029)
                     /gene="galU"
                     /locus_tag="ACG8PO_002808"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFQ0208060.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
BASE COUNT          918 a          637 c          468 g         1011 t
ORIGIN      
        1 ttccactact ttttattttt ctgacactac atattcttat aactcatttc tcataactgt
       61 gaaacaactt ataaaaataa caaactttaa acaaaacaag cttaaaatca aagatgatcc
      121 atcttaaaat aactaaaaca aaaaaagtag tcgttgttaa tcctcattaa taatcgtttt
      181 ctttttttca aacgctccat ttttatgtgg aacattattt ttttaaggaa ctttgtcaac
      241 atgtggataa ctattttcca cttgtgtcct ggactagctg ttttgctgta aaatacaaaa
      301 cttgctgcag caaaaagatc gttgcaagag aggctgttcc gcagatcgct ggatctagat
      361 ttggcaaagt caagactgca tcatgtaaat tggcgtggtt gtccaaaacc aaatcagcaa
      421 attcaaataa tcgcaaacca ctgctatgtt gcgatttgat acttcgtgac aaatcaaatc
      481 cagtaacaat gattaatgga tgaccatttt ctttgatcca ctgagctagt tcgatgattg
      541 ccgggtttct tccttcattc gaaagaagaa gaaaaatgtc ttctgatttg acagaaagct
      601 gatcaataat tgcctgtcct gcaccttcta gcgtttcata gacacctccg gctggatttt
      661 caatcaatat acttggtaca agatccgggg tttgatccca aaattcaaat gctacaccac
      721 ttagtgtgcg gctagcatat atctgcaatc taccaccttg attaatttgt ttcgcgatcc
      781 aagtgccagc ttgaagcatc agcggctctt ctatccttaa tttctcttcc aattggtgat
      841 aatagtcaaa ataacataaa ctcatttatc tctcccgcct agctatactt gacatgaata
      901 gaaacagctg cctaatctaa ggaagtacat aaaaaaacag tagttagaca gtaacacatg
      961 tggtctttgc tgtttcaaac acaaaaaagg cattgaacga atacgcccaa taccttgaca
     1021 tagcaaataa aacaacgtgc agaccatgga aaatatctac ataggggtag aattttcacc
     1081 ctagattagg tacaataaat taggtcgatg cactgcattg actatggcaa aatgatcggt
     1141 cagggtgtat gtgtgtgact ggtcattccc tgattcttag cgccaactaa gggtcaggga
     1201 gccttttttt attcagctgt ttctatctga atttattgta agtaatctct tatccaagtg
     1261 caagcaagaa aatacaaaaa aacattggta tgatttatat atatataaat aacataaaaa
     1321 aaggaatggc ttatcccgat acaacaagca ttcctttaaa aaagattctt ttatataagt
     1381 gaaaaaagct tttttccacc aattaaataa atctctcatc tcaatagttt atccattatt
     1441 ttatcttttt ttgcgtatac ctactaaata ttaccgtctt cttctcgttc ttctactttg
     1501 ttcgagagaa tctgtactag aaaaagtagt tgtatgtcta ccttttctgc ctatccgaga
     1561 ctttcttttc atatatggga tataaatgat tagatacagt acagcaaaaa ataaagcaaa
     1621 tacgcaagct tcccaactac ctaacgccaa ttcaaccatc aaggtagcaa ccccaacgga
     1681 tgctgcatac cagataaaaa tcagaaacgt ttcttttttt ctgaccatgc gaaacatgat
     1741 aaatagacta ataagcaata gtggcaacac aaatatccaa ttgcttcctt ccatcagcca
     1801 cttgagtata tcaagcagcg actgtgcaac agcgactaat tttcccatag tgaacacaca
     1861 ccttttttta taattaaaaa attcgccttt ttattctact atgaatcttc tggcttggct
     1921 ttcatttctc ttatgtaatc attttctttt atacaactta aaaaaaaatt aaaccattag
     1981 cataatattt tctgaatcta ttcacgaaag tctttttcta tctttcgttt tccacgactc
     2041 aactgatcta gagtcaaaaa actaaaacga cagttgcgca aatgacgcac aagctgccgc
     2101 tttaataaca aatagccttt cattctcagc aggccactta tttccagtta actttctgtt
     2161 cttagctttt tactcaactg gataatatat gcacttaatg aagtcccaat ttccgaatgt
     2221 tgaagtccat attcaatatt tgtcttcata tatcctaact tatcgcctac gtcgtatctt
     2281 tttcctttaa actctctcgc atagaccgcc tctgtctggt taagagtctg aagtgcatcc
     2341 gttagctgta tctcgtttcc tgctcctggt tcttgagttt ctaatatctc aaatatttta
     2401 ggagtcagta gataacgtcc gataatggct aaattacttg gtgcttttcc catttcaggt
     2461 ttttctacaa acccttttac tctgcaacga cccttttcgc tatctgtttt aggatcaata
     2521 attccgtatt tagatgtttc attttctggt acacgcatga cggcaacagc ggaagcttgt
     2581 gtttcttcat aatccatcat caattgttga gtcaatggga tatcatcttt catcaaatca
     2641 tcccccagca tgacgacaaa cggttcattt cctataaatg cttttgcttg aagcacggca
     2701 tcccccagtc ctcttggatg agattgacgg atgaagtgaa gattgacttg agtcgtttct
     2761 tcaactaaag cgagtaattc tgttttttcc ttttcccgca aattgttttc caattcgata
     2821 tttgcatcaa agtgatcttc aatgggtctt ttttcttttc ccgttacgat caagatgtct
     2881 tcgattcccg aatccaatgc ttcttccaca ataaactgga tcgttggttt atccacgatt
     2941 ggaagcattt cttttgccat ggcttttgtt gccggcaaaa aacgtgttcc tagcccagcg
     3001 gcaggaatga ctgctttttt gaccttcact tgct
//
LOCUS       DAZKKW010000169         3012 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.173, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000169 DAZKKW010000000
VERSION     DAZKKW010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3012
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.173"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..921
                     /locus_tag="ACG8PO_002809"
     CDS             <1..921
                     /locus_tag="ACG8PO_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HFQ0208061.1"
                     /translation="LAVSFLVLAGCGKQASDQADEGSKEKLSVVATNSILADMAKEVG
                     TDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSWFDNLMETAKK
                     EEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARIFSEKDAENAT
                     LYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAYDLPAAYIWEI
                     NTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKIYDKLFTDSIA
                     KEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            1062..1814
                     /locus_tag="ACG8PO_002810"
     CDS             1062..1814
                     /locus_tag="ACG8PO_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0208062.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            1833..2984
                     /gene="queG"
                     /locus_tag="ACG8PO_002811"
     CDS             1833..2984
                     /gene="queG"
                     /locus_tag="ACG8PO_002811"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HFQ0208063.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
BASE COUNT         1085 a          491 c          664 g          772 t
ORIGIN      
        1 ttggcagtta gtttcttggt attagccggt tgcggaaagc aggcttctga ccaagcggat
       61 gaaggaagta aagaaaagtt gtcagtcgtg gctaccaatt cgatcttggc ggacatggca
      121 aaagaggtcg gaacagatca aatagatatc cacagtatcg taccagtcgg aacagatccg
      181 catgaatatg aagtactacc agaagacatc aaaaaggcaa gcgatgcaga tgttatttta
      241 tacaacggtt tgaatcttga aacaggtaac agctggttcg ataacttgat ggaaacggct
      301 aaaaaagaag aaggaaaaga ttattttgca gttagcaaaa atgtagaacc tctttattta
      361 actagcggtg aagaacatac aaaagcagat ccccatgctt ggctagacct atctaacgga
      421 ataaaatatg tggaggaaat cgcacgtata ttctctgaaa aagatgcaga aaatgcgaca
      481 ctctataaaa aaaatgcaga agcatatgtg gaaaaactaa aagaattaga tacacaagcg
      541 aaggaaagtt ttgcttctat cgaagagaac aaaaaattat tagtaacaag tgaaggggca
      601 ttcaagtatt tttcacgagc atatgatttg ccagcagctt atatatggga gatcaataca
      661 gaaagtcaag gtacgcctga tcaaatgaaa gcgatcattg atcagataag agcaaaagaa
      721 gtaccggttt tattcgtgga aaccagtgta gactcaagaa gcatggaacg ggtagcaaaa
      781 gaaacgggtt tgaaaatcta tgataaactg ttcactgatt ccatagcaaa agaaggagaa
      841 caaggagatt cttactatca gatgatgaaa tggaatattg aaacgatcca tgaaggtttg
      901 tcacaaacaa aagaaagctg aaaagtgcaa ggtctagctt gaggagcaga ccttgcattt
      961 tttataagag atatgaaaaa aaattatgct gtttttcgtc gtacagatac cttttttgaa
     1021 gaaattccgt tatagttaga gtaaggaagg aaggtgtggc tatgaatttt acacgtaaag
     1081 ataaatatac gattaaagaa gaagtctttc gattgatcag ccagcaggaa aataccgaca
     1141 aggatcaaga ccttcaattt cttgaagcag aattagctaa attattcata gcaaatcaaa
     1201 atgagacaac agaaccagat gaagagctgt acataggcaa atggttgttt gatgaatttc
     1261 agcctttaga tgaaaatacc ggtatcggtt attttgtgtt cctttctcca gtcggcttca
     1321 cacctggcgt attagttaaa caaaacgacg aaataaccgt caaagcatgg cctgcattat
     1381 tttcagtcat caaagcagaa ggagtaatca aaaagatcca gtcggcctat tataaaagtc
     1441 aatttattgc caacagcaaa ttaatgaata tgttttataa tcgtctagta gaaattgcta
     1501 ggcctgggaa aatcccgcaa acattcacgt acacacagca aaaaaatctc tatgaagaag
     1561 tattgtattc ccgcgatctg tctgagtttt cgccatggga agaggtaagg ctccatgaac
     1621 tgttaagtac aaaaccagtt tcattgaatt tttttaatgc attagataac aaaagaaaat
     1681 taatttcaac aaattattgg gaagtatttg ctgcaagaaa aaaatggatg gaagaaaaga
     1741 agattgccga tcagcagatc cagtttgtcc agctgaaaaa agaaaaacta tattatgggt
     1801 tatacagccg ttaacagcaa gcgaggaaga ccatgacatt aaaagaagaa atcatacaag
     1861 aaagcaaacg attaggaatt gacaaaatcg ggttcaccac ggcggaacct tttgattcat
     1921 taaaagaatc gttggaagaa caaaaagcag ctggccatac ttctggcttt gaacatccaa
     1981 atatcgatga gcgtctttat ccagaaatga catttgatca gccgaaatcg atcatttcca
     2041 ttgcacttgc gtatcctact aggatccatg aagaagtgcc tagggatgaa aaacgcgggc
     2101 agtttgctcg tgcttcttgg gggatcgatt accatgatat actcagagac cgtttgaacc
     2161 gattgatcga attcatccaa acaagagcag aaaaatggca aaaagaagag gaatggcgtc
     2221 tagctccgca agtagatact ggagaactga tcgatgtcgc tgtagcacag cgagctggtt
     2281 taggatttat tggacgaaac ggattgttga tcactgaaga gttcggttca tttgtctatc
     2341 ttggtgagat cgttactaat attgtatttg agcctgataa accaggagaa tttggttgcg
     2401 gtgactgtat gcgctgcgtg actgcttgtc cgacaaaagc attgcttggt gacggaagaa
     2461 tgaatgccca gcgatgtttg tcttatcaaa ctcagacaaa agggatgatg ccggaagaat
     2521 atcgtaaaaa aatgcgaaat gtcatttatg gatgtgatat ctgccagtta gtctgccctt
     2581 acaatcaagg aaaagatttt cattttcatg aagagatgga accaaaagta gacgaaatat
     2641 atccaaaatt aaagccaatg ctttcgatgt ctaataaaga cttcaaacag caatttgggc
     2701 atttagctgg ttcatggcgt gggaagaaac ctttgcagag aaacgcattg attgctttag
     2761 ctaatctagg tgatcgaacg gcattgcctg agatttcaga atgtttaaca gatgtccgcc
     2821 ctgtcatcag aggaacagca gcttgggcta ttggacgtct aggaacaaaa gaaccggaaa
     2881 aatggcttga tgtattagat gaaatattag ctaaagaaac agaagaagtc gttataaacg
     2941 aattggaaca tgcccagaaa ttattgcgga gaaagttaaa ataaaaaaat agttaacatt
     3001 attcaaagtt tt
//
LOCUS       DAZKKW010000170         2957 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.174, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000170 DAZKKW010000000
VERSION     DAZKKW010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2957
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.174"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..761
                     /locus_tag="ACG8PO_002812"
     CDS             <1..761
                     /locus_tag="ACG8PO_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFQ0208064.1"
                     /translation="DLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRKKQ
                     AFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKIIYE
                     EVAKEEKVTPEAIMAIHKTENNLQLTNQLVSAHKVNAFFGQQLSVNDSIEAINLKDIC
                     REVAKELKVCERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFVPEVK
                     KDWNDQLKHEIQQQEIRKQKRAMLFQQGRQQERE"
     gene            772..>2957
                     /gene="topB"
                     /locus_tag="ACG8PO_002813"
     CDS             772..>2957
                     /gene="topB"
                     /locus_tag="ACG8PO_002813"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFQ0208065.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFPGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFKVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQVGADQGKTFKRLWINSLEKEAIYQGFQQLRDAKETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FKKEPYFEGKSEVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGMIRQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLRAEEISTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTEATYES
                     LTPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAIGKVPIDLGWKKLFGK
                     ESDGKEKEEEPLLPKVTKGEMVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGVLLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGERKGTQTTFLANIQKFVSHLLEVVPGQIQSIDFGSTLQEVKA
                     ASEKQEAARHLGICPKCQEQEVLLYQKVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNSRTSQPVKGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNFKKRT"
BASE COUNT         1074 a          540 c          649 g          694 t
ORIGIN      
        1 ttgatttact tagctatgct gcactgaatc gtgatcagtt aaacgatacg tggttagtct
       61 ctatggaagg gttaaaacac catgtgatca gtcattattt tggtgaggca gtatctgaat
      121 tgagaaagaa acaagccttt cctcaatcca tcgagatttg tgtggataat gaccgtgcag
      181 gacatatttt ctacgaaaaa gaacaattaa tgggcgcagt cgatccattt acgaatcaga
      241 aagtacgttg tgaacgagga atcgctaatg attggcaagt gccaaaagaa tacaaaatca
      301 tttatgaaga agtcgcaaaa gaagaaaagg tcacaccaga agctattatg gccatacaca
      361 agacagaaaa caatctgcaa ttaacgaacc aactggtttc cgctcacaaa gtaaatgcgt
      421 tttttggcca gcagctttct gttaacgatt caatagaagc cattaatttg aaagatattt
      481 gtagagaagt cgcaaaagaa ttaaaagttt gtgaacgcgt ggatggtacg tacgattttg
      541 atcgttttta tcaagaaaaa ggggatatta atgcgcagat tcttttctct tataaagcag
      601 aacaatacta caaaggctat aaaaatcatg aacatgaatt tgtacctgaa gtaaaaaagg
      661 actggaacga tcaattgaag catgagatcc agcaacaaga aatcagaaag caaaaacgtg
      721 ccatgttgtt tcaacaaggc agacaacaag aaagggagtg aaatcgtcat gatgaaaacc
      781 gttattttag cagaaaagcc gtcacaagcg aaagcttatg ccgatagctt ctctaaagcc
      841 actcgaaaag acgggtattt tgaaatccaa gatcgattgt ttcctggtga aactgtgatc
      901 acgtatggtt ttggtcattt agtcgaatta gattcgcccg atatgtacga tgaaaattgg
      961 aaacaatggt cattagaaca tttgcctatt ttccctacac attatcacta tcatgttcct
     1021 aaagataaga aaaagcaatt caaggtggtg aaacagcaac ttcaatcggc cgacaccatt
     1081 attattgcga cagatagcga tcgtgaaggg gaactgatcg cttggacgat cattcaacaa
     1141 gtaggcgccg atcaaggaaa aacctttaaa agactttgga tcaactcctt agaaaaagaa
     1201 gccatttatc agggattcca gcagttacga gacgcaaaag agacctatcc aaaatttgaa
     1261 gaagcacaag cacgtcagat cgccgattgg ttaattggga tgaacggtag cccactgtat
     1321 agtttactct tgcagcaaaa aggcatacca ggaagctttt ccttaggcag agtccaaacc
     1381 ccaacgcttt acatgattta tcaattgcag gaaaagatta gaaatttcaa gaaagaacct
     1441 tattttgaag gaaaatccga agtaatcgct caaaatggcg catttgatgc gaagcttgat
     1501 ccgaatgaaa cccaagcgac gcaagaagca tttgaagatt atttaaaaga aaagggggtt
     1561 caattgggaa aacagccagg catgattcgc caagttgaaa ccgagaaaaa aagcgcggca
     1621 agtccacgtc ttttttctct atccagcctt cagtctaaaa tgaatcagct catgaaagct
     1681 agtgccaaag acactttgga agccatgcaa ggcttgtatg aagggaaata tctaagctat
     1741 ccgcgaacgg atacccctta cattacggaa ggggaatatg cgtatctgtt ggatcactta
     1801 gacgaataca agcatttttt gagggctgaa gaaatttcga ctcctattca tacaccgaat
     1861 agtaggtacg tgaacaacaa gaaagtccag gaacattacg caatcattcc tacaaaaaca
     1921 gttatgacag aagcgacgta tgaaagtctg acacctttac aacaagcgat ttacgagcaa
     1981 gttttaaaaa cgacagtggc gatgtttgcg gaaaaatata cctatgaaga aacaacgatc
     2041 ttgacgcaag tccagcagct tcagttaaag gcaatcggaa aagtaccgat agatctaggt
     2101 tggaagaagt tatttggcaa agagagcgac ggaaaagaga aggaagaaga gccgctactt
     2161 cctaaagtga caaaaggaga aatggtgacg gtagatctac aagtccttga aaaagaaacc
     2221 aagccaccac aaccttatac cgaaggtacg ttgatcactg cgatgaaaac agctgggaaa
     2281 accgtggata gtgaagaagc gcaatccata ttgaaagaag tggaaggaat cggtacagaa
     2341 gcgacacggg cgaatattat tgaaaccttg aaacaaaagg aatatatcaa ggtagagaaa
     2401 aataagcttg tggtgacgaa taaaggggtt ttgctttgcc aagctgtgga aaaagagccg
     2461 ctgcttacga gtgccgaaat gaccgccaaa tgggaaagct acttgttaaa gattggagaa
     2521 cgaaaaggaa cacaaaccac ttttctagcc aatatccaaa agtttgtctc tcacttattg
     2581 gaagtcgtcc caggacaaat ccaatccatt gattttggat cgacgttaca agaagtgaaa
     2641 gcagccagtg aaaagcaaga agccgcgcgt catttaggaa tttgtccaaa atgccaggaa
     2701 caagaagtct tactctatca aaaggtggca gcttgtacgt ctgaagcctg tgattttaaa
     2761 ttatggacaa cgattgccaa aaagaaactc acagcgacac agctgaagga gattattcaa
     2821 aatagccgaa caagccaacc agtgaaaggg ttaaaaggtc agaagggttc gtttgaagca
     2881 acgattgttt taaaagaaga cttcactaca ggctttgaat tttctgaaaa gaaaaaaacg
     2941 aacttcaaga aaagaac
//
LOCUS       DAZKKW010000171         2914 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.175, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000171 DAZKKW010000000
VERSION     DAZKKW010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2914
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.175"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(45..1364)
                     /locus_tag="ACG8PO_002814"
     CDS             complement(45..1364)
                     /locus_tag="ACG8PO_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFQ0208066.1"
                     /translation="MKKLKIFPKMFIQIFSVLGIIIILVHSLIFFIFPKTYLETRKEK
                     IHNMANEISSNMNGKEIKYIEQTLEFYSKSSEIKVFIKEKNNKNEIQIKDNINVNLES
                     DSNSLIIEEREIKLNDGKKTNLQFVSTADMQKDAKDLSLKFLPYSLLISILFSAIISL
                     IYAKSIKNNIQEIKIVTDKMMKLDKKMSLKVSSNDEVGELKQQINDLYSTLLRTIDDL
                     EFKNKEILKLEKLKYDFFKGASHELKTPLASLKIILENMKYNIGKYKERDIYINECIE
                     IVDSLTKNISQILSVHSIENLNNDEEYLKINDTLEDILKKYEILANQKKITVNNYILD
                     ENVYIGKTALKIILSNLISNAVKYTDVNGVINIGIVNDWLYIENSYGNNKISNMDKIF
                     DVKFDLNKENSNGLGLYIVSNILNNYNIEYKVLQDEEFFIFKIKIFS"
     gene            complement(1361..2014)
                     /locus_tag="ACG8PO_002815"
     CDS             complement(1361..2014)
                     /locus_tag="ACG8PO_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFQ0208067.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
     gene            2302..>2914
                     /locus_tag="ACG8PO_002816"
     CDS             2302..>2914
                     /locus_tag="ACG8PO_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HFQ0208068.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGRLDDSP
                     SHCEHCGSIRIIRNGSYTTRTQILKVKEKLTILELKRTRFLCYDCGQTFSAKTDLVDE
                     HHQLTKELKQAILIELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSMKSVSGSMSFICVDGTTHQLFTILEDRRLYKLTQYFL"
BASE COUNT          955 a          474 c          321 g         1164 t
ORIGIN      
        1 ataagtctct atttttcaat aactattgca aaattatatc ttaattaaga aaatattttt
       61 attttaaaga taaaaaattc ttcatcctgc aatactttat attctatatt gtaattattc
      121 agaatattac ttacaatata taaacctaat ccattactat tttccttatt taaatcaaat
      181 ttaacatcaa atattttatc catattcgaa attttattgt taccgtatga attctctata
      241 tataaccaat cattaactat cccaatatta attaccccat ttacatcagt atattttacc
      301 gcattactaa tcaaattaga tagaataatc tttaaagctg tttttcctat ataaacattc
      361 tcgtctaata tataattatt gacagttatt ttcttttgat ttgctaatat ttcatacttt
      421 tttaatatgt cttctaatgt atcatttatt ttcaaatatt cttcatcatt attcaaattt
      481 tctatagaat gaactgataa tatttgagaa atattttttg ttaaactatc aacgatttca
      541 atacattcat taatataaat atctctttct ttatattttc caatattgta tttcatgttt
      601 tccagtatta ttttaagact agcaagaggg gttttaagtt catgggatgc acctttaaaa
      661 aagtcatatt ttaacttttc taattttaaa atttctttat ttttaaattc caaatcgtca
      721 attgttctta ataaagtaga atacaaatca ttaatttgtt gttttaattc tccaacttca
      781 tcattagagc taacttttaa actcattttt ttatcaagtt tcatcatttt atcagtaaca
      841 atttttattt cttgtatatt attttttatt gattttgcat aaattaaaga aataatagca
      901 gaaaataaaa ttgatattaa taaagaatat ggtaaaaatt taaggcttaa atctttcgca
      961 tctttttgca tatcagctgt agaaacaaat tgtagatttg tttttttacc atcattaagc
     1021 tttatttctc tttcttcaat tatcaaagaa ttactatcac tttctaaatt aacatttata
     1081 ttatctttga tttgtatttc attcttatta tttttttctt ttatgaatac ttttatttca
     1141 ctactcttgg aataaaactc caaagtttgt tctatatatt ttatttcttt tccattcata
     1201 ttactagaaa tttcatttgc catattatga atcttttctt ttctcgtctc caaatatgtt
     1261 ttaggaaaga taaaaaaaat taatgaatga actaatataa ttattattcc aagaacagaa
     1321 aatatttgta tgaacatttt tggaaatatc tttaattttt tcattttctc tccaatttat
     1381 atcctacatt tcttatagta gttatacagt ctaattgtaa tttttttcta agttctttta
     1441 tatatacatc tattactcga tcataaggag tttcttcgct atctttccat acataatcaa
     1501 taatttgcat tcttgttaac acttgtccat tattatccaa taaacatttt aatacttcta
     1561 gttcttttgc atttacatct atttcttcac catttatttt agctgtatag ctattaaaat
     1621 taaccgatag atccttatat tcaaacttct ctaaatgtcc ataattcttc ttaattaaag
     1681 aatctattct agcttttaag accggcaatg aaaatggctt ttctacatat ccgtctacta
     1741 aattagtaaa tgcatcaatt ttatattctt catcactaaa tgcagtcaag attagaattg
     1801 gtaaattgct tttctttcta atttctttca acacttctaa accatttata aaaggtatct
     1861 gaatgtctaa gataaccaaa tttatatcgc tattaaattt tgacaaggct tctcttccat
     1921 ctttagcttg aataacagta tatccaaatt ctgaaagata ttcacttatt ccctctctta
     1981 tcatattatc atcttcaaca gttaatattt tcatatatac ctccacttaa gcctatacac
     2041 tagatattat acagcatatt atggctctat aatatctagt gttggtgaca ccttctgttg
     2101 ccaatacttt ttcttttttc taataaatgc aaacagacac cttgaaatcc tttagaataa
     2161 agggttctat aatattttgt gttgttggaa aatcgaaggc gattttctac taacactttt
     2221 tcttcgtttc aactcaaaaa gagactcact tttggtaaaa tttagtcgac caaaacatca
     2281 ccctaccaaa ggagtcgctc tatggataat catactagaa aattactcgg attaacagat
     2341 aaacatttat tttttgatga agaatggctg attgaaaaag aatataaagg cgttcaagcg
     2401 cagtttatta aagggagatt ggatgactca ccttcccatt gtgaacactg tggatcgatt
     2461 cgtattatcc gcaacggttc ctatacgaca cgtactcaga tattgaaagt caaagaaaag
     2521 ttgactattt tagaattaaa acgtacccgt tttctatgct atgattgtgg gcagaccttt
     2581 tctgcgaaaa cggacttagt tgatgaacat catcaactaa caaaggaact gaagcaggcg
     2641 attctcatag aactgtacga aaatcaatca cgtaaactaa tcgctaagaa gtacttcgtc
     2701 tcggatggga cggtgacacg gatcttgcgt gaagcaacga agcattacca gccacgtatg
     2761 aacttccttc cgacggtgtt gtgtatggat gagttcaaat caatgaagtc tgtttctggt
     2821 tccatgagtt ttatttgtgt tgatgggaca acccatcagc tatttacgat ccttgaagat
     2881 cgcagacttt acaaactaac tcaatacttc ctgc
//
LOCUS       DAZKKW010000172         2902 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.176, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000172 DAZKKW010000000
VERSION     DAZKKW010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2902
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.176"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1741
                     /locus_tag="ACG8PO_002817"
     CDS             <1..1741
                     /locus_tag="ACG8PO_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0208069.1"
                     /translation="DNSSILKIELKKENNKSMKISKKAFLLVVLILLSTLYSVNFMRN
                     AQEIYTTGDLSFHLSRIKGLSSIFEGPINYTTFNNYGDGLNYFYPFLTIIPAVVFYGI
                     SNNLILSYVLYIWLLNICTILISFYYGEKFFKKIDAAFLFSCLYTFATYRTVDIYYRS
                     AIAEAIALTIIPAVLYYAYGLIFNREKKVIALSVSISLLVYTHVLTTLLCSALIACMI
                     IVRLFLSKQIKNDIKFVVVNLVKAVFLTCALTSYFWYPMLQQMNYQEIHSPFKTDLQL
                     EALSISDSIIQALNNDITTYTMGLLGLVALIFPLFILKEMEKKEKIIYVGVVSTWVCV
                     TNLFPWFLFQKTPISLLQFPWRILGIQIFFSSMIITLVFMKKTMNKKYSWLIIGATVV
                     FLLVTSIAAKENYAKVIQSKHGHIVINEETLPRYTTSDMGGLYDYAPTEAIKERPHLE
                     KHEVKVGESWEKGKYKAADNNITYTVASNKKQKVTVPVYAYLGTQVKVNGKVVNDSYK
                     KHGLVNVDVNAGENKIEITTKYTPTALMALWISILTYCLVVYSSFKRFAPNKLKDSRY
                     VTKKEINKKNENK"
     gene            1728..>2902
                     /locus_tag="ACG8PO_002818"
     CDS             1728..>2902
                     /locus_tag="ACG8PO_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-pyruvoyl-tetrahydropterin synthase-related
                     protein"
                     /protein_id="HFQ0208070.1"
                     /translation="MKINKKVVILSGLIIVSSIYSFIFFKNTQSIYTTGDLSFHLSRI
                     KGLSTIFTSPINYETFNYTGYGVNYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNL
                     CTTLVAYHYGERFLKQKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFY
                     AYQIISGKEEKYPSVKLALSMSLLVYSHVLSTLMSTALIIIFIFIRLVSKGFKNADFI
                     AIFKKLFSAAGMTLVLTSAFWYPMFEQMLYQKINKPSVTNLYAHASNVFDSLTEAMNN
                     DLTTYSMGLVGLLSLCIPLILFKKLTNIEKKIYYGTCLTWLATTSLVPWYLLQNTPAK
                     LLQFPWRILSLQIIFSSLILTMIFFKNRRYNKTRELFYLFMTVLLLITLTVSSKKNF"
BASE COUNT          980 a          425 c          473 g         1024 t
ORIGIN      
        1 agacaattct agtatactga aaatagaatt aaaaaaggag aacaacaaat ctatgaaaat
       61 aagtaaaaaa gcatttcttt tagtagttct tatcttactt agtactttat attcagtaaa
      121 ttttatgaga aatgctcaag aaatttatac aacaggtgat ttatcttttc atctttcacg
      181 aatcaaaggg ttatcttcta tttttgaagg gccaatcaac tatacaacat ttaacaatta
      241 tggagatgga ctgaactatt tttatccttt tcttacaata attcctgcag tcgtttttta
      301 tggaatttca aataacttaa tattaagtta tgttctatat atttggttat taaatatttg
      361 tacgattctg attagttttt attatggaga aaagtttttt aaaaagatag atgctgcttt
      421 tttatttagt tgtttatata cttttgcaac ttatcgaaca gttgatatct attatcgatc
      481 cgcaattgca gaagcaatcg ctcttacgat tatcccagct gttttatatt atgcttatgg
      541 attgatattc aatagagaaa aaaaagtcat cgctttatct gtcagtataa gtctacttgt
      601 gtatactcat gtcttaacta cactattgtg tagtgcattg attgcttgta tgattatagt
      661 tcgtttgttt ttaagcaagc aaataaaaaa cgatataaaa tttgtggtcg tgaatttagt
      721 aaaggctgtt tttttgactt gtgcattaac tagctatttt tggtatccta tgttgcagca
      781 gatgaattat caagaaatac acagtccctt caaaacagat ttgcagctag aagcactttc
      841 tatttcagat agtatcattc aagcgttaaa taacgatatt acaacataca ctatggggtt
      901 attaggattg gttgcgttga tttttccttt attcatactg aaagaaatgg agaaaaaaga
      961 aaaaatcatc tatgttggcg tagtttctac atgggtatgc gtaactaatt tatttccatg
     1021 gtttttgttt caaaaaacac cgataagctt actccagttt ccttggcgta tcttaggtat
     1081 ccaaatcttt ttttcttcaa tgatcataac gcttgttttt atgaaaaaga ctatgaataa
     1141 aaaatattca tggttaatca ttggcgctac tgtggtgttt ctactcgtca cgtctatagc
     1201 agcaaaagaa aattacgcaa aagttatcca atcaaaacac ggtcatattg ttatcaatga
     1261 agaaacactc ccacgttata caacatcaga tatgggagga ttatacgatt acgccccaac
     1321 agaagcgata aaagaaaggc ctcacttaga aaaacatgaa gtaaaagtag gagaatcgtg
     1381 ggaaaaaggg aagtataaag ctgcagataa taacattact tacacagtgg caagcaacaa
     1441 aaagcaaaaa gtgacagtac cagtatatgc ttatttaggt acacaggtaa aagtaaacgg
     1501 taaagtcgta aatgactctt ataaaaaaca tggcttagtg aatgtggatg taaatgctgg
     1561 agaaaataag atagaaatta caacaaaata tactccaact gctctcatgg ctttatggat
     1621 cagtattctg acttattgtt tagtggtgta ttcaagtttt aaacgatttg cacctaataa
     1681 gttgaaagat tctcgatatg tgactaaaaa ggagataaac aagaagaatg aaaataaata
     1741 aaaaagtagt aatattgagt ggattaatta tagttagtag tatatattct ttcatctttt
     1801 tcaaaaatac acagagtatt tataccactg gagatctttc ttttcactta tctcgaatca
     1861 aggggttatc gactattttt accagtccga ttaattatga gacattcaat tatactggat
     1921 atggtgtaaa ttatttttat ccctttctga ctttttttcc agctgtaatg ttatattgga
     1981 tgacaaaaaa tttgattgta agctatatta tttacgtttg gttattgaac ttgtgtacaa
     2041 cactcgtagc ttatcattat ggcgagcggt ttttaaaaca aaaaaaagcg gcatttttat
     2101 ttagttgtct ctatattttc tctgcttatc ggacagtaga catctactat cgttccgcaa
     2161 ttgccgaagc tatcgcaata acacttgtta tccctgtatt attttatgct tatcaaatca
     2221 tctctggaaa agaagaaaag tatccgtctg taaagctagc attgagtatg agtttgctag
     2281 tgtacagcca tgtactatca acattaatga gtacagcatt aattatcatt tttattttta
     2341 ttcgtttggt ttctaaaggt ttcaagaatg cagattttat tgctatattt aaaaaattat
     2401 ttagtgcggc aggaatgaca ttggttctga cgagtgcttt ttggtatccg atgtttgaac
     2461 aaatgctcta ccaaaaaatc aacaaaccta gcgtgacaaa tttatatgct catgcatcta
     2521 atgtgttcga tagtctgact gaagctatga acaacgactt aactacttac tcaatgggac
     2581 tagttggtct gttatcttta tgtattcccc tgattttatt taaaaaatta acaaacatag
     2641 aaaaaaagat ttattatggt acgtgtttga cttggctagc tacaactagt ttggtgccat
     2701 ggtatcttct gcaaaatacg ccagccaaat tgttacaatt tccttggcgg atcttaagct
     2761 tgcaaataat attctcttct ctgatattaa cgatgatatt tttcaaaaat agaagataca
     2821 ataagacaag agaactattt tatttattta tgactgtttt gttattaatc actttaactg
     2881 tttctagtaa aaaaaatttc aa
//
LOCUS       DAZKKW010000173         2853 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.177, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000173 DAZKKW010000000
VERSION     DAZKKW010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2853
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.177"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1412
                     /locus_tag="ACG8PO_002819"
     CDS             <1..1412
                     /locus_tag="ACG8PO_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Rib/alpha-like domain-containing protein"
                     /protein_id="HFQ0208071.1"
                     /translation="PTVEGEKVEVGGTVDLTDNVTNLPALPEGTTVTDVTPGGTIDTN
                     TPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSDADKYEPTVEGEKVEIGGTVDLTDNV
                     TNLPALPEGTTVTDVTPGGTIDTNTPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSNA
                     DKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYAWKEKVDVSTAGNKKGTIVVTY
                     PDGSSDEVEVDVTVTDTRSDADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYT
                     WKEKVDVSTAGNKKGTVVVTYPDGSKEEVEVTISVVDKKAPNKPQVDPITEGDQIVTG
                     KTEPNAEVTVTLPDGSQHHGTADKNGNFTVKVPKLEAGTKVIVTATDESGNTSEPTNV
                     VVSSNKKDSGKNGSKGSKTDNQGSNSNQDKNRGKSQSSKVFPKTGESDSNIFTISGGL
                     ILLGTLGLLGYKNRKKENE"
     gene            1546..>2853
                     /locus_tag="ACG8PO_002820"
     CDS             1546..>2853
                     /locus_tag="ACG8PO_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFQ0208072.1"
                     /translation="MEKIVIIGSNHSGLAAARFLNLSDSNYEVILIDKKSEISYLECG
                     TSLFIKGEVSNLEDLFYQNRMTLNSEIKKLFLETEVDKIDIQNKKISCVKKDGTRFSQ
                     EYDKVILATGSKQIMLDIPGNNLANIFSIKNFPNAKEIFKALDDSTIKKVGIIGAGYI
                     GVEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYL
                     GRKKIEKIVTTSGTQYSMDMVVQAIGFLSNSPIGEKELLRDVSGAYLTNEYQQTNIKD
                     IYAIGDCATTFFTSINRSSVDFSISNALRTAYIATQHINNQDFTPSGSQFTNGFSIFN
                     YNFYAAGLNLKTARLLSESIDYIEIEDFIRPAFLRKNSRVKLRISFEKNTKRIVGVQL
                     CSQEDLSGLLSMFSLAIEEQITLDKLKMLDYLFHPSFSQPYNFI"
BASE COUNT         1118 a          391 c          559 g          785 t
ORIGIN      
        1 aaccaacagt agaaggcgaa aaagtagaag ttgggggcac agtagatcta acagataatg
       61 ttactaactt gccagcgtta ccagaaggaa caacagtaac agatgttact cccggcggta
      121 caattgatac caacacacca ggtaattacg aaggtgtcat tgaagtaacg tatccagacg
      181 gtacgaaaga tacggtgaaa gttccagtag aagtgacaga caatcgttct gacgccgata
      241 aatatgaacc aacagtagaa ggcgaaaaag tagaaattgg aggcacagta gatttaacag
      301 ataatgttac taacttgcca gcgttaccag aaggaacaac agtaacagat gttactcccg
      361 gcggtacaat tgataccaac acaccaggca attatgaagg tgtcattgaa gtaacgtatc
      421 cagacggtac gaaagatacg gtgaaagttc cagtagaagt gacagacaat cgttctaatg
      481 cagataaata caatcctgaa ggacaaaaag tgactacaga cttaaataaa gagccagatg
      541 catctgaggg aattaagaac aaagaagatt taccaaaagg aactacttat gcttggaaag
      601 agaaagtaga tgttagcaca gctggaaata aaaaaggtac tattgtagtg acatatccag
      661 atggatcatc tgatgaagtt gaagtagacg ttacagtgac agatactcgt tccgatgcag
      721 ataaatataa tcctgaagga caaaaagtga ctacagactt aaataaagag ccagatgcat
      781 ctgagggaat taagaacaaa gaagatctac caaaaggaac tacttatact tggaaagaaa
      841 aagtagatgt tagtacagct ggaaacaaaa aaggtactgt agtagtaaca tatccagatg
      901 gatctaaaga agaagtagaa gttactattt ctgtagtaga taaaaaagca ccgaataaac
      961 ctcaagttga tcctattaca gagggtgacc aaattgttac tggtaaaact gaaccaaatg
     1021 cagaggtgac agttacatta ccagacggaa gtcaacacca tgggacagct gataaaaatg
     1081 gtaattttac agttaaagtt cctaaattag aggcagggac aaaagtgata gtaactgcaa
     1141 ctgatgaatc tggtaatact agtgaaccta ctaatgtagt tgtttcttcc aacaaaaaag
     1201 acagtggaaa aaacggtagt aaaggtagta agactgacaa tcaaggtagc aacagcaatc
     1261 aagacaaaaa tagaggcaaa agccagtcct caaaagtttt tcctaagacg ggagagagtg
     1321 acagtaatat ctttactatt agcggtggtt taatactgtt aggaacgtta ggattattag
     1381 ggtataaaaa tcgaaaaaaa gagaatgaat agaaaatcaa tttcttaagt cttctctgag
     1441 aagaggaaga atctttttct tcctcttttt gcttttttat gatgggaaat aagtgttagt
     1501 taaagatgaa tatacttttt atagaaagga aaagaatgta attaagtgga aaaaatagta
     1561 attattggtt caaaccattc agggctagcc gcagctcgat ttctaaactt aagcgattca
     1621 aattatgaag ttattctaat cgataaaaaa tcagaaatta gttacttgga atgtgggaca
     1681 tcgttattta taaaaggaga agtaagtaat ttagaagatc ttttttatca aaatagaatg
     1741 acactaaatt ctgagataaa aaagttattt ctagagacag aagttgacaa aattgatatt
     1801 caaaataaaa agattagttg tgtaaaaaaa gatggtacaa gattctctca agaatatgac
     1861 aaagttatat tagctacagg ttcaaagcaa attatgctag atataccggg gaataattta
     1921 gctaatattt tttcgataaa gaattttcca aatgcaaaag agatttttaa agctttggat
     1981 gatagtacta ttaaaaaagt aggaattatt ggagcaggat atattggagt tgaaattgct
     2041 gaagccataa gaaaaagagg gaaagaagta tatcttttcg atgttgcaga tagagtgttg
     2101 tcgacttatt acgaccgtag tttttcagat aaagttgaag aaatactatc aaaaaatgga
     2161 atacatcttt gtctatcaga aagtcctaaa aaatatttag gaagaaaaaa gattgaaaaa
     2221 atagtcacta caagtggtac ccaatactct atggatatgg tcgtacaagc tataggattt
     2281 ctatctaata gtccaattgg ggaaaaggaa ctcttgagag atgttagtgg agcatatcta
     2341 actaatgaat accagcaaac aaatataaaa gatatatatg ctattgggga ttgtgctaca
     2401 acttttttta cgtccattaa tagaagtagt gttgattttt ctatttctaa tgctctaaga
     2461 acagcttata ttgcaactca acatattaac aatcaagatt ttacaccttc tggttcccaa
     2521 tttacaaatg gcttttctat atttaattac aatttttacg cagcaggttt gaatttaaaa
     2581 acagcacgat tattaagtga gtctatagat tatatagaaa tagaagattt cattagacct
     2641 gcttttttga gaaaaaatag tcgtgtaaaa ttaagaatta gctttgaaaa aaatacaaag
     2701 agaatagtag gtgtacaact ttgtagtcaa gaagatttat ctggactatt atcaatgttt
     2761 tctcttgcta tagaagaaca aattacatta gacaaattga aaatgttaga ttatttattt
     2821 catcctagtt ttagccaacc ctataatttt att
//
LOCUS       DAZKKW010000174         2828 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.178, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000174 DAZKKW010000000
VERSION     DAZKKW010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2828
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.178"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..154
                     /locus_tag="ACG8PO_002821"
     CDS             <1..154
                     /locus_tag="ACG8PO_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HFQ0208073.1"
                     /translation="SLEKLGCQNFNSRSVKGLESRFESILLAYSVLLSRAQRRFEGTL
                     RYSLGY"
     gene            complement(208..486)
                     /locus_tag="ACG8PO_002822"
     CDS             complement(208..486)
                     /locus_tag="ACG8PO_002822"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0208074.1"
                     /translation="MENKVGYVRYNFFSSTPIMKDFASFNEALAVELSHDRERIHYEK
                     HVIINDLWNTEKTDLLALPDTDYPVFKEIEVKANKYNEIKQNKITNLS"
     gene            complement(601..1218)
                     /locus_tag="ACG8PO_002823"
     CDS             complement(601..1218)
                     /locus_tag="ACG8PO_002823"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0208075.1"
                     /translation="MLTMTDINTIRNLRNNHDKSINHIAKELDVNWRTAKKYADDDLL
                     PQPKARKKTGMMYTEKWGGIVSLWLSEDAKLPNKKRRTAEAMTTALKADGFPGSYRTV
                     CVFIQDWKATHYLETLEDQGFERLEHPPAEAQLDFGTMEVCHDGAFKDVKVLVMTFPF
                     SNAGFAVVLPAENQECLLEGMKELFSQSGGVPRKIRIDNMSTAVT"
     gene            complement(1613..2437)
                     /locus_tag="ACG8PO_002824"
     CDS             complement(1613..2437)
                     /locus_tag="ACG8PO_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFQ0208076.1"
                     /translation="MEIKVLFENSRINLNFKSGHGLSLLINYSGKKYLFDAGANKKLV
                     YNATKMGETLNDLEAVIISHGHSDHTGGLRYLKSGNAPIYTAEGVTLPHYLKILTTFL
                     YVGIPKNEILKMHENLHTIKTKTELAPSFHIIPLGKANSTTKNLYKKMDDKFVYDDFS
                     DELLVVIEQEDGLIVFTGCSHHGIIDMMEIALETFPTKPIKYLIGGFHMIGIPYINNL
                     GLPEQEVMKIGNKLNDSRVEKILTCHCTGSKAFNILKTQLGNKLEAIKTGQHLEIS"
BASE COUNT          813 a          531 c          523 g          961 t
ORIGIN      
        1 ttccttagaa aaattaggtt gtcaaaattt caactcacgt tctgttaaag ggctagagag
       61 tagatttgaa agcatattac tggcttacag tgttctatta agtcgagcac aacgacgttt
      121 tgaaggaact ttgagatatt ctttaggata ttaaaattaa ctagcaccac gggtaaaata
      181 aatagttttt ctatcaagta tattgaacta cgataaattt gttattttgt tttgtttaat
      241 ttcgttgtac ttattggctt tgacttcgat ttctttaaat actgggtaat cggtgtccgg
      301 caaagctagc aaatctgttt tttctgtgtt ccatagatca ttgataatga catgtttttc
      361 gtaatggatt cgctctcgat cgtgtgacag ctccaccgct aatgcctcgt tgaaactggc
      421 gaaatccttc ataatcggtg tagatgaaaa gaagttgtac cgtacatagc cgactttgtt
      481 ttccatacta cctttctcgt ttccgctacg tggattgcac acctgtgtct caaaaccata
      541 gtgcatagaa aatcggagga aaccgtcagt cagcaccgct tgttcagtct ttgtttttgt
      601 ctaagtaacg gccgttgaca tattatcaat ccgtattttc cgaggaacgc cacctgattg
      661 gctgaagagt tctttcatgc cttccaacaa acattcttga ttctcggcgg ggagtacaac
      721 ggcgaatcct gcattactaa aagggaaagt catcactaat accttcacat ctttaaacgc
      781 tccatcgtga caaacttcca tcgtaccaaa atcaagttgc gcttctgctg gcgggtgttc
      841 aagacgctca aagccttggt cttccagtgt ttccaagtag tgtgtcgctt tccaatcttg
      901 aatgaataca cagactgttc ggtaggatcc tggaaagcca tcggctttta aagcagtagt
      961 cattgcttca gcagtcctac gctttttatt tggcagctta gcatcttctg aaagccatag
     1021 ggaaacgatg cctccccact tttctgtata catcatacca gtctttttgc gtgctttagg
     1081 ttgcggcagc aaatcatcat ctgcatattt tttagccgtg cgccaattta catctagctc
     1141 ctttgcaata tgattaatgg atttatcatg attatttcgt aaatttctga tagtattaat
     1201 atcggtcatt gtgagcatac ctttcttacc tccagttaca gtgtcgagac tataacttta
     1261 gaggagtttc aggttgcccg caatggcttt ttatcttaac ttaatcgatt tgcggagttg
     1321 tgtagttttt cgttgcactt gtatgtactt ttattctgcc ataaacagct taatgaatgt
     1381 ccacttgaaa aagtgaataa acgaaataga tgactcgatt taataaggaa gtaaacccac
     1441 acagaaatat aggtgatttt ccttgaatga ccaagatatt gaaagttagt gaaaaaacac
     1501 aaaaaggtag ccctattcac agaaggtctg ccttttgtgt tatcgccagc aaacattaga
     1561 gggggtattt catatagctt ttgtcctctc gacagtccta tcagtttctc ttttaactta
     1621 tctctaagtg ctgtccagtt ttaatagctt ctagcttgtt tcctaactgt gtttttaata
     1681 tgttgaaggc ttttgaacct gtgcagtggc aagtaaggat tttttctact cgtgagtcat
     1741 tcaatttatt tccaattttc attacctctt gctcaggtag acctaaatta tttatataag
     1801 gtatgccaat catatggaag ccaccaatta ggtattttat gggtttagtc ggaaatgttt
     1861 caagagcaat ttccatcata tcgataatgc catgatgact acagccagta aagacaatca
     1921 gaccgtcttc ttgctcaatt acgaccaata attcatctga aaaatcatca taaacaaatt
     1981 tatcatccat ctttttgtat aaattcttgg tggtcgagtt tgctttgcct agggggatga
     2041 tatggaagct cggagcaagt tctgttttcg tttttattgt atgtaaattc tcatgcattt
     2101 taaggatttc atttttagga ataccaacat acagaaacgt ggttaaaatc tttaaataat
     2161 ggggtaaagt tacaccttca gctgtataaa taggggcgtt tcctgatttt aaatatctta
     2221 acccgccggt gtggtcagaa tgaccgtgtg aaataatcac tgcctccaga tcattcaaag
     2281 tttcccccat tttagtcgca ttatagacta attttttatt agcgccagca tcgaagagat
     2341 atttcttccc tgagtagtta attaagagag ataacccatg tccactttta aagttcaaat
     2401 ttattcttga gttttcaaat aaaaccttga tttccactat aacatctcct ttcacatgaa
     2461 cgtgtattca tatattgtag cacattaagt tctatttttt gttgtaatta ccgaaaaaaa
     2521 tattaatgga cgctttttac gctttatttc gtatgtggaa gaaaatttct taggatgtgg
     2581 cgggggattg gggcaaagct aaattccaat tacagtgatc atttaattag acacctttaa
     2641 atacccacac tttcatcaac actataattt agggaaagaa aagtgttggc atgatataga
     2701 aaaaccttta attaacagcg tttcctaaaa ttggcacgca tcttgcttat agaaaagcgt
     2761 aaggatagga ggaatgaaaa tggcaactat aattcttgcc agccatgggg aatttgctca
     2821 aggattaa
//
LOCUS       DAZKKW010000175         2762 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.179, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000175 DAZKKW010000000
VERSION     DAZKKW010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2762
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.179"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            56..478
                     /locus_tag="ACG8PO_002825"
     CDS             56..478
                     /locus_tag="ACG8PO_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0208077.1"
                     /translation="MAKVIDKHIYFYETHIDVLDEIIATHAEVKNYSEAVRFLCMNYD
                     SEVEKKVNSNQAKLNAMGKEISILTEMVSEFADLHLKDTGILIGTNSTVYQEAKERVE
                     GKIKANQTKKYSPKIKKTISESVTTKSEVFDPTRFLKR"
     gene            498..2087
                     /gene="mobP2"
                     /locus_tag="ACG8PO_002826"
     CDS             498..2087
                     /gene="mobP2"
                     /locus_tag="ACG8PO_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobP2 family relaxase"
                     /protein_id="HFQ0208078.1"
                     /translation="MSKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEKERRTPEE
                     EQELRLIKNALEDFYIPEGETFSESKTGKEFSETSNEAQSLMKHGINFDKSEEQDFTK
                     YLSYMTRHYALEQKKVLTHSEQKEYQRLNQAIKKYQTEPHKKASGNLPGVFSMASDEV
                     KGSDLKEIKQTFQRGQQKGSIIYQDVVSHDHSYLEKIGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKKNTRKPYTEIENGVSYIVPKG
                     KRRQATLDEMKTNYVHELERFSYEKNRMGMEKRNLLTRKSDLRNTLTKVVKEPVRYDR
                     HAMSLLTEVYKNLPKKRSEWHYGDENRTKLSPYTREKLDELTQYVLTKEPGYQEYIQI
                     SKALKEEAKSLYGESKRESKDAEKNALFDLKKRTGNAILTELKKQDSQIKPLIERMDQ
                     MQSIYNERSFRTLKQLPTNEYKYSYEEFMKRRKQFQQRVVTQRSLRQLNRTINAHEEK
                     YHASKAYEEQQRRIQMEQERQ"
     gene            2099..2434
                     /locus_tag="ACG8PO_002827"
     CDS             2099..2434
                     /locus_tag="ACG8PO_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0208079.1"
                     /translation="MKQYLDQWKVIEGSLREERIEQLPDCLEKEHLFQIREMLINEQF
                     DPNQFLVVEYPATGVYCCNHVKGEKYFIIQEYEGKLAPYYTTWEMNEEGINNFPCKSI
                     EESISLTEC"
     gene            2575..>2762
                     /locus_tag="ACG8PO_002828"
     CDS             2575..>2762
                     /locus_tag="ACG8PO_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0208080.1"
                     /translation="MAYSTIGVVVEKSRDNLVFVTEIQTGRAFVVTDKAAKAYQSGDI
                     LTLNLTTKTFVDAAEDYP"
BASE COUNT         1098 a          417 c          560 g          687 t
ORIGIN      
        1 aataatcaaa ttatcgaact tttaattgaa caaagtagag gaggagaagt ttaacatggc
       61 caaagttata gacaagcata tttattttta tgaaacacat atagatgttc tagatgaaat
      121 tattgcaact catgcagagg ttaaaaacta tagtgaagcc gttcggtttc tatgtatgaa
      181 ttatgattca gaagtggaga aaaaagtaaa ttccaatcaa gcaaagttga atgcaatggg
      241 aaaagaaatt tctattttaa ccgaaatggt ttcagagttt gcggatctcc atttgaaaga
      301 tacagggatt ttaattggta caaattcaac tgtttaccaa gaagcaaaag agcgtgtgga
      361 aggcaagata aaagcaaatc aaacgaagaa atattctcct aagataaaga aaacaataag
      421 cgaatcagtg actacaaaaa gtgaagtatt tgatccaaca aggttcttaa aacgataaaa
      481 attagaagga gaatgtgatg agtaaatcac ctgcagtcat tctcacttgt caatttacct
      541 tgcccaataa aaaatctttt tcaacataca ttgattatat gacaagagaa aaagcgctgg
      601 aagaaaagga acgacgtacg cccgaggaag aacaggaact gcgtctaatc aagaatgctt
      661 tagaggattt ttatattcct gaaggagaaa ctttttcgga gagtaagacg ggtaaagaat
      721 tcagcgaaac gtcgaatgaa gcgcaatcgt tgatgaaaca tggaatcaat tttgacaagt
      781 cagaagaaca agattttacc aaatatcttt cttatatgac acgtcactat gcgttagaac
      841 aaaagaaagt actgacgcat agtgaacaaa aagaatacca acgcttgaac caagcaataa
      901 aaaaatatca aacagagcca cacaaaaaag cttcaggaaa tcttcctgga gttttttcta
      961 tggcttcaga cgaagtgaaa ggaagtgatc tgaaggagat caagcaaacc ttccaaagag
     1021 gacaacaaaa aggctcaatc atttatcaag atgttgtctc tcatgatcat tcgtatcttg
     1081 aaaaaatagg attgtacgat cccaaaacag atactttaga tgaagaaggt ttaaaagcag
     1141 ctggaagaaa aatgatggag acattgttcg agaaagagca actgaatgaa acaggctact
     1201 ggatggcaac gattcaccga aataccaaac atatccatat tcattttgca gttgttgaaa
     1261 aaaaaaatac gcgtaaacca tacactgaga ttgaaaatgg tgtgagttat atcgtaccga
     1321 aagggaagag aaggcaagca acattagacg aaatgaagac taattatgta catgagttag
     1381 aacgtttttc ttatgaaaaa aatcgaatgg gtatggagaa acgaaattta ttgacacgga
     1441 aaagtgattt gcgtaacacc ttaaccaaag tagtgaaaga gccagtacgt tatgatcgac
     1501 acgcaatgag cttattaact gaagtatata aaaacttacc taaaaagcgt tcggaatggc
     1561 attacgggga tgaaaatcga accaaacttt caccatacac aagagagaaa ctcgatgagt
     1621 taacccaata tgtgttaaca aaagaacctg gatatcaaga atatatacag atttcaaaag
     1681 cattgaaaga agaagcaaaa agtctttatg gggaaagcaa gagagaatca aaggatgcag
     1741 aaaaaaatgc tctctttgat ttaaaaaagc gcacaggaaa tgccattcta acagaactga
     1801 agaaacagga tagtcaaata aaacctttaa tagaaaggat ggatcagatg caatctattt
     1861 acaatgaacg atcctttcga acacttaaac agttgccaac gaatgaatac aaatatagtt
     1921 acgaagaatt catgaagcgc aggaaacaat tccaacaaag agttgtcacg caacggagtt
     1981 tgcgtcaatt aaatcgaacg attaatgccc atgaagaaaa ataccacgca agtaaagcat
     2041 acgaagagca acaacgaaga attcaaatgg aacaagaaag acagtaggag gaatagcaat
     2101 gaaacaatat ttagatcaat ggaaagttat agaaggatca ttaagagaag aacgaatcga
     2161 acaacttccc gattgtttag aaaaagagca tttgtttcag ataagagaaa tgttgataaa
     2221 cgaacagttt gatccaaatc aatttttagt cgttgagtac ccagctactg gtgtgtactg
     2281 ttgtaatcat gtgaagggtg aaaaatattt tatcattcaa gaatatgagg gcaagttagc
     2341 gccttattat actacttggg aaatgaatga agaggggatc aataacttcc cgtgtaaaag
     2401 cattgaagag tctatttcac tcacagaatg ttagttgtta agtgaataaa agaagtaaaa
     2461 gagtcctgaa cgtgattgtc caggactctt ttttttcact gaattgtttt ccgccaattc
     2521 attgagtggt gcgatttccg tctacattat atcaaaagaa aggaggaaga agaaatggct
     2581 tattcaacga ttggagttgt tgttgaaaaa agcagagaca atcttgtttt tgtgacagaa
     2641 attcaaacag gcagagcttt tgtagtcaca gacaaagcgg caaaagcata tcaaagtggc
     2701 gatattttaa cgttaaatct gactacgaag acgttcgtcg atgcggcaga agattatcca
     2761 tt
//
LOCUS       DAZKKW010000176         2715 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.180, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000176 DAZKKW010000000
VERSION     DAZKKW010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2715
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.180"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            271..834
                     /locus_tag="ACG8PO_002829"
     CDS             271..834
                     /locus_tag="ACG8PO_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000017403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0208081.1"
                     /translation="MSGYIYGYARVSTRSQELNRQLDLLNEQNCNEILTEKMTGTKAV
                     RPQLNRLKDKIRPGDTVIVESFSRLGRSTKDLIDLVTYFEEHHVKLVSLKENFDTSTP
                     HGRLMMTVFQAFSQFERDLIVERTKEGLKSARARGRKGGRPRVNQKDVDRAVKLYKSQ
                     VYSVKEITEMTGISKATLYRYLKDNRE"
     gene            complement(1113..1400)
                     /locus_tag="ACG8PO_002830"
     CDS             complement(1113..1400)
                     /locus_tag="ACG8PO_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000312842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFQ0208082.1"
                     /translation="MAYEILPSKHVIKYLKKLKEKTLKEQFLTIIYDEIAVRPHSGEQ
                     KTGDLSGIWAIGFKYAGTTYRVAYEIKDNAVIPILLCGTHENFYEQLKKIR"
     gene            complement(1390..1719)
                     /locus_tag="ACG8PO_002831"
     CDS             complement(1390..1719)
                     /locus_tag="ACG8PO_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFQ0208083.1"
                     /translation="MITKTRKQGNSIMLTVPKEFDVPNGVEVEAKLVENGILYEFVEP
                     KKEFFDFSEDILADILSEGYSKQEILKEFRNRKNALTSAFKSIAEDTVANSKPMTKEE
                     LAAEIGL"
     gene            2024..2353
                     /locus_tag="ACG8PO_002832"
     CDS             2024..2353
                     /locus_tag="ACG8PO_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
                     /protein_id="HFQ0208084.1"
                     /translation="MKKVCQVTVVHKEKVNGIKNKLDQKDFSNLLVLGKCFSDFSRIK
                     IFYALETYKEMCVCDLAEILTASVATTSHHLRFLKKHGMAKSRQDGKVVYYSLANEDI
                     LSAIRVF"
BASE COUNT          918 a          426 c          439 g          932 t
ORIGIN      
        1 taattttcat tgtgataaac tcgttattaa ctttaggata cactagttag gattaaatta
       61 ggtatctaga aatttactta agattttttt gtttatttcg tatacacact cgtatcaaaa
      121 acaatcgatt atgagacaaa atgaaatgac ctcttttatt aaataaaaga ggtcatattt
      181 tgtatcataa cttgttgcat taactatgta tctataactc ccttttatga tacaatcaag
      241 ccagaattaa agtacgaaag gaagaaaagc atgagtggat acatttatgg ttatgcccga
      301 gtgagtacac gttcacaaga actaaatcgt caattagact tactcaatga acaaaattgt
      361 aatgaaatcc taactgagaa aatgacggga accaaagcgg ttcgtcccca actaaatcgt
      421 ttaaaagata aaattcgtcc gggagatacg gtaattgtag aaagtttttc tcgattgggt
      481 cgtagtacaa aggatttgat tgaccttgtt acctattttg aagaacacca tgttaagctg
      541 gtgagcttga aagaaaactt cgataccagc acgcctcacg gacgcttaat gatgactgtc
      601 ttccaagcat ttagtcagtt cgaacgtgat ttaattgttg aacgtaccaa agaaggtttg
      661 aaaagtgcac gtgcgagagg tcgtaaaggt ggccgtccac gagtgaacca gaaagatgtt
      721 gatcgagcag taaaattgta taaaagtcag gtttatagcg taaaagaaat tacggaaatg
      781 actggtatta gcaaggcaac actctaccgc tacttaaaag acaataggga ataatctatt
      841 ttaatcttta ggaatctata atatttatgg gagaattaga gtgatttttt tattattact
      901 aaaaaacaat ataaaataac gcattttaag ccctctttgt gcataaggat gaagctttta
      961 atttgaaaga ggcttaaaat gcgttttaat tttttctatt tttcataaat atataggtta
     1021 gtggtttaaa attatagggt attacaactc tcagctgtta aaaattatct tttaaagtgt
     1081 cgtctatcct ctcaagttga aacattgggt tcttacctaa tcttttttaa ttgttcataa
     1141 aaattttcat gcgttccaca tagtaagata ggtatcactg cattatcttt aatttcatat
     1201 gcaacgcgat atgtcgtacc tgcatacttg aatccaatag cccaaattcc tgataaatca
     1261 cccgtttttt gttctcccga atgaggacga actgctatct catcataaat tatcgttaaa
     1321 aattgttctt ttaatgtttt ctcttttaat ttttttagat actttattac gtgtttagaa
     1381 ggtagaatct cataagccaa tttctgctgc caactcctct tttgtcatcg gtttagaatt
     1441 tgcaacagta tcttcagcaa tagatttaaa tgcagaagtt aacgcatttt ttctatttct
     1501 aaattctttt aagatctctt gtttgctata accttctgaa agaatatcag ccaaaatatc
     1561 ttcactaaaa tcaaaaaatt cttttttagg ctctacaaac tcataaagga taccattctc
     1621 tacaagctta gcttctactt caacaccatt gggcacatcg aattctttag ggaccgttaa
     1681 cataatagaa tttccttgtt ttcttgtttt tgtaatcata ttatttcctc cattatttta
     1741 catttcgtaa ttacttagta attacattgt acgctatttt tagtagaaat acaaaataat
     1801 aaaactcctt tagaacaatt ttcaaaggag ttttatttga ttaggaaatt atgtagaata
     1861 ataatatgtt tttaacttgg aatcaaaaac gcacttgcaa gtaagtttac ttcaatacat
     1921 gaaattgctt ataatagtgt attggaatta aggtagattg acaaaagagt gatcaaatag
     1981 gtatacttca ttcaaataat cgtttgaatg tgaggtgaga tgaatgaaaa aagtttgcca
     2041 agtgactgtc gtacataaag aaaaagtaaa tggaataaaa aataaattgg atcaaaaaga
     2101 tttttcaaac ttacttgtgt tagggaaatg tttcagcgat ttttctagaa ttaaaatttt
     2161 ctatgcttta gaaacctata aggaaatgtg cgtctgtgat ttagcagaaa tacttactgc
     2221 tagtgtcgct acaacgtcac accatttacg ttttttaaaa aaacatggaa tggcaaaatc
     2281 acgtcaagat ggaaaagtag tttattactc tttagcgaat gaagacatcc tttccgctat
     2341 tcgtgttttt taaagttatc agaaaatcta tctatgaaat cttagtttgg gggtaccgtc
     2401 aggaaaatgc ggatttacaa cgttaaggaa atttattaaa gagaccaatt cctcagcact
     2461 aaaattgata attgggcttt ctttgttact atagtgttag gtaagctttt tactttatca
     2521 ccggttttcc cctcacttca cacctacggg cgagtttccc cacatcccgt tttccatcta
     2581 atccaattca ttcaatatct ttatgtgtat gaaagatcag attgcttgat ttagacatct
     2641 tacacgtaat ttattcttaa atgggtacgg taaacgagca ataatacgag caaaagtata
     2701 cctacaaaaa taata
//
LOCUS       DAZKKW010000177         2715 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.181, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000177 DAZKKW010000000
VERSION     DAZKKW010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2715
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.181"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            149..1552
                     /locus_tag="ACG8PO_002833"
     CDS             149..1552
                     /locus_tag="ACG8PO_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFQ0208085.1"
                     /translation="MKKSLKLASAVILMSQLLIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSELLTTSSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESSTISS
                     TESSTTSSSTSEITSSKVTKPNHIESSKKEAPKTEGQTKTFSTPQQIESTESFQSQPI
                     LSGNEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIAQAILESASGQSQLAQA
                     PNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYENYEESFEDYAKLLTKGI
                     SGNKDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNELIKTYDLTNYDKEVKGP
                     QMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIHAVKAGKVVKAEFHYSW
                     GNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGSTGNSTGPHLHLEICKDS
                     SLSQSMLVDPKTLLFGD"
     gene            1901..2533
                     /locus_tag="ACG8PO_002834"
     CDS             1901..2533
                     /locus_tag="ACG8PO_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HFQ0208086.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLTGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
BASE COUNT         1033 a          382 c          521 g          779 t
ORIGIN      
        1 tttaaaggca gtaacaggat taaacattga aacataatcg ttactaaatg aaatcaagaa
       61 atatactgta ttactcaatt cattgaaaat atttatcata atttttaata gaaaacacta
      121 ttaaagagaa aaaagagagg taaatcaaat gaaaaaatct ttaaaacttg catcagcagt
      181 tatattaatg tcccaactcc ttatagcaac acctttccat gtgttggcaa gcgaaaattc
      241 aacatacaaa attgcaaaag aaagaaccga gacgactgaa atgagtaatt ccgatgtaac
      301 agcagagagt agtgaacttt tgacaacttc atcaagtgaa agtagtacga cttcaaatgc
      361 cgagtcttca acaacttcat caagtgaaag tagtacgact tcaaatgccg agtcttcaac
      421 aacttcgtca agtgaaagta gtacgatttc aagtaccgaa tcttcaacaa cttcaagtag
      481 tacaagtgaa ataacttctt cgaaagtgac aaaaccaaac catatagaat cttcaaaaaa
      541 ggaagcgcca aaaacggaag gacaaacaaa aactttttcg acaccacagc aaatagaatc
      601 aacagaatct ttccaatcac aaccaatact atctggtaat gaagaaattc attttgacaa
      661 aaatgaatct gtagaaaatt ttattatgaa aattggtgag tctgcgagaa aaattggtca
      721 gaaatatgat ttatatgctt ctgttatgat tgcacaagcg atcttagaat ctgccagtgg
      781 tcaaagccag ttagctcagg caccgaatta caatttattt gggataaaag gaacttataa
      841 tggaaacttt gtcattatgg taacaaacga agatttgggt aatggaacgc tttatacgac
      901 ccaatcaaaa tttagagtgt atgaaaacta tgaggaaagc tttgaagatt atgccaaatt
      961 attaactaag ggaattagtg gaaataaaga tttttatgca ggagcattaa aagctaatag
     1021 taaaacatat cgagaagcaa ctaaattcct tacaggacgg tatgcaacag atacacaata
     1081 ctatttgaag ctaaatgaat taattaaaac ctacgattta accaactatg ataaagaagt
     1141 taaaggtcca caaatgagta agactggata ccaagtgcct attcataatt attcaataac
     1201 cagtttgttt ggaaatcgtg gaaatgaatt tcaccgagga cttgatatgg cagctacaca
     1261 gggtgagccg attcatgcag ttaaagctgg taaggttgtt aaagcagagt ttcactactc
     1321 ttggggaaat tacgtggtga tacaacacga agatggatca accgcgttat atgctcacca
     1381 gcaacaatac ctagtaaaaa ctggagataa tgttttgcaa ggacaaataa ttggctatgt
     1441 aggaagtact gggaatagta ctggcccaca ccttcatttg gaaatttgca aagatagtag
     1501 tctttctcag agcatgttag ttgatccgaa aaccttatta ttcggagact aaaaaaggta
     1561 atcaatctga caaagtaaca agaaaatggt tggattctta gacctttgat caggatattg
     1621 agactggaat tgatatctcg attacgatgg gtttgacaaa taaaacaagt ccattatcga
     1681 atatcgagtg tattattgcc gtctttatgc tcatattttg agcaggtttc actagatgaa
     1741 aacaatttat ctatcttgag gatttcgtgt acgtatcgtt cagtcttgta ttgtaaatta
     1801 gtcataaaat cagactaaga aaaattggaa atattttttt ctgatttatt aaaagttaat
     1861 tgaaattata tggtacttct ttcttgggag ggtaggtgca gtgaaggaaa gatatggtca
     1921 ccttactgtt gttgaggaga ttactcataa tggatataaa aaatatcgtt gcctatgtga
     1981 ctgtgggaat gaaacgattg tatatcgatc aaacttacta acaggtagaa ccaaaagttg
     2041 tggatgtgga aattataaaa ataggcataa atataaaaat ttgacaaata aaacttttgg
     2101 taagttagtt gcaatcaagc caactaaact gcgaagtgaa ggaactgttg tttgggaatg
     2161 tcaatgtctg tgtgggagaa aaaagttagt atcggcaaag cgacttctaa aaggtgatgt
     2221 aaaaagctgt ggatgtttga aatcaaaaaa gcatcacatt gaaaataaaa aattcggatt
     2281 acttactgca aagtatcctt tggacaataa ctgttcatgg aaaacaaaat gggtttgtca
     2341 ttgtgagtgt ggcagtatgt gtgagataag ttatagtaac ttattttttg gacatactca
     2401 aagttgtgga tgtttaaaaa ggcaagaaac aaggacatgt gttgaaggga cagtaataga
     2461 gtgtctaacg tcaaaattac cgaaaaataa tacaagtggt gttaagggag tttatcgttc
     2521 taaagagaaa tagattgcgt atataacttt aaaaaaaaag aggtactatt taggatcgta
     2581 tgattctatt aagagtgcag ctcaagctag aaaagatgct gaaaataaat tgtttcaacc
     2641 tttgattgac aaatatcgag gtaatcatta attattgtgg ataataatta acagtgatat
     2701 ataaacatct ataat
//
LOCUS       DAZKKW010000178         2667 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.182, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000178 DAZKKW010000000
VERSION     DAZKKW010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2667
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.182"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            751..2577
                     /gene="ltrA"
                     /locus_tag="ACG8PO_002835"
     CDS             751..2577
                     /gene="ltrA"
                     /locus_tag="ACG8PO_002835"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFQ0208087.1"
                     /translation="MLKKNKLRYNEYYDMQHIYDELYAQSKNGNNFYKLLEIIGSEQN
                     ICLAYRNLKSNSGSKTAGTDGMTIDDIKQLSNAEIVATVRESLSNYRPKSVRRVFIPK
                     AGSDKMRPLGIPCIWDRLVQQCILQVLEPICEPKFHNHSYGFRANRSAHHAVSRVTTL
                     INLSKYHYCVDVDIKGFFDNVNHGKLLKQIWTLGIRDKRLICIISKMLKAEIDGEGVP
                     EKGTPQGGLLSPLLSLIVLNELDWWVSSQWETFQPKNRSKNGWLQYAKKYTKLKSGFI
                     VRYADDFKIMCSTYGEAQRFYHSTVDFLNKRLKLEISPEKSKVVNLKKNSSDFLGFKI
                     KVIPKGRTKHGYVAKTDMNQKALKKAKTNLKLKVKDIARHTTGFNISRYNLTVIGMQN
                     YYCIATNVYNNLTEVSYALLPTIRIRLRNIAKSVPFESTSSEFQSRTKGIRPKTKIVM
                     IADNPLLPIQGVQHKNPMNFSQDICNFTKQGRNKVHEDVVVVTKEEIRALLENENPAD
                     SVEFNDNRISAYIAQQGNCYVMNRRGTPSTLICIHKSDKGDNLDRYSNLAFVEIPIAK
                     AILTESADEAKSLLKGYVLNSQQKKKLNRIRTNYGYQTITGK"
BASE COUNT          955 a          466 c          593 g          653 t
ORIGIN      
        1 aaagggggtg cgaaacgttc ctacttgaaa agagtaggca cagcacttca ttaaatcaat
       61 atttaatgtg tgttggagaa ctaaacacac gaatagtcga atgacggggt aacgccctga
      121 aaggctactt taatactctg actggcagaa acttgagtaa acaaggctct gtcagaagct
      181 cggtgaagtc ggcagacaga caccgtaaca gataatgctg acgaaagatt cccagaggtg
      241 ggcgttgtgt cctatatgcc ggaagtctat aaaatatcta tggttagaat gtagatgaga
      301 ggtctactga cgaatccttg aatgtacagg tctatatcgg ggctggatag aaatgtccag
      361 tgacacaaac agtgtcgcta ctgtgggtga gtaagactcc gcatttatga aaatccatat
      421 aacgttaaag gcgttatcaa ggtagcaggc ttatagagga aacctaagga tatatgtaca
      481 gatagtgtgt ttggaacgtt gaaagctgac aacatggaga ttttactgtc tgtgaagtgt
      541 tcacatcaga aagttattgt gagattagca atgatataag gtgtgttaat gtcagtgaga
      601 gtggtggcac agtacctatg aaaccgtgat aacaagcggt ggagggatag ccactagtca
      661 tatgaagaat agaattacta agaaaaatgg tatgggttcg agtaagacta agagatgtac
      721 ttgctcctgc aaacaaggag ggtacagacc atgctaaaga aaaacaagct ccgctataac
      781 gagtattatg atatgcaaca tatttatgac gagttatacg cacagagcaa gaacggtaat
      841 aacttttata agttacttga aattattggt tcggaacaaa atatttgttt agcttaccgt
      901 aatctgaaat caaacagtgg cagtaaaacg gctggaacgg atggaatgac gatagacgat
      961 ataaaacaac tcagcaatgc tgagattgtt gctaccgttc gggaaagcct tagcaactac
     1021 cgccccaaat cagtcagacg tgttttcatt ccaaaagctg ggagcgataa aatgcgtcca
     1081 ttggggattc cgtgtatctg ggacaggctg gtacaacaat gtatcctgca agtcctagag
     1141 ccgatatgcg aaccaaagtt ccataatcat tcctatggat tccgtgcgaa tcgaagtgcc
     1201 caccatgcag tcagcagagt aacgacactg ataaatttat caaaatatca ttactgtgtt
     1261 gacgtagaca ttaaaggatt tttcgataat gtcaatcatg gaaaactatt aaaacaaatc
     1321 tggacacttg gtattcggga taaaagattg atttgcatta tcagcaaaat gctgaaagcc
     1381 gaaattgacg gcgagggtgt cccagaaaaa ggaacgccac aaggcggtct gttaagtcca
     1441 ctgctctccc tcatagtcct taatgaatta gactggtggg taagcagtca gtgggaaaca
     1501 ttccagccga agaaccgcag taaaaatggg tggcttcaat acgcaaagaa atataccaaa
     1561 ctgaaaagcg gttttattgt acgttacgct gatgatttta aaatcatgtg ttcaacctat
     1621 ggagaagcac aaagatttta ccacagtacc gtagattttc ttaataaaag gctgaaatta
     1681 gaaatcagtc cagagaaatc aaaggtagta aatctgaaga aaaattcatc agattttctg
     1741 gggttcaaaa tcaaagtcat tccaaaagga agaacgaaac acggttatgt tgccaaaaca
     1801 gatatgaacc aaaaagcatt aaagaaagcg aaaacaaatc taaagttaaa ggtaaaagat
     1861 atagccagac atacgactgg cttcaatatc agcagatata atctgacagt tatcggtatg
     1921 cagaattact actgcatagc aactaacgta tataacaacc tgacagaagt aagttacgct
     1981 ctcttaccga ccatacgaat ccgtttaagg aacattgcaa aatcagttcc atttgaaagt
     2041 acaagttcag aatttcagag caggacaaaa ggaatcagac caaaaacaaa aatagtgatg
     2101 attgcggata atccgctatt gccgattcaa ggagtacaac acaaaaatcc tatgaatttc
     2161 tcgcaagata tttgtaattt tacaaagcaa gggagaaaca aggtgcatga ggacgttgta
     2221 gttgtaacaa aagaagaaat ccgagcgttg ttggaaaatg aaaaccctgc cgatagcgta
     2281 gagtttaacg acaatcgtat ttcggcatat atcgcacaac aaggcaactg ctatgtaatg
     2341 aatcgtcgag gaactccgtc cactctgata tgtattcaca aatcagacaa aggagataac
     2401 cttgacagat attctaatct ggcatttgtg gaaattccca ttgcaaaggc aatcctaaca
     2461 gaatcagcag acgaagcaaa atcactgtta aaaggttatg ttcttaatag tcagcagaag
     2521 aaaaaactga acagaatacg gacgaattac ggataccaga caataactgg gaaataactt
     2581 ataaatctat ttggatatga tggaacgccg tatgaagcga aagtttcatg tacggtgtga
     2641 agcgggggaa aacttggaga taacatc
//
LOCUS       DAZKKW010000179         2484 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.183, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000179 DAZKKW010000000
VERSION     DAZKKW010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2484
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.183"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..300)
                     /locus_tag="ACG8PO_002836"
     CDS             complement(<1..300)
                     /locus_tag="ACG8PO_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6056 family protein"
                     /protein_id="HFQ0208088.1"
                     /translation="MNKHNRKITNNKELILLLIGIWGIFLLFNMYMPTMRADDVVYAS
                     RLDELGYLGASIEHYKTWSSRIIIELFLMFFSKHFMLWKLLNSTIMLGIVVLLCKY"
     gene            393..742
                     /locus_tag="ACG8PO_002837"
                     /pseudo
     CDS             393..742
                     /locus_tag="ACG8PO_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741963.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(1014..1304)
                     /locus_tag="ACG8PO_002838"
     CDS             complement(1014..1304)
                     /locus_tag="ACG8PO_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0208089.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(1512..2204)
                     /locus_tag="ACG8PO_002839"
     CDS             complement(1512..2204)
                     /locus_tag="ACG8PO_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFQ0208090.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQAITDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT          734 a          458 c          349 g          943 t
ORIGIN      
        1 atacttgcaa agtaatacta caattcctaa cataatcgta gaatttaata atttccatag
       61 cataaagtgt ttagaaaaga acattaagaa tagttctata ataattcttg aactccatgt
      121 cttataatgt tctatcgaag cacccaaata gccaagttca tctaatctac ttgcataaac
      181 tacgtcatcc gctctcatag tcggcatata catattaaag agtaaaaaga taccccaaat
      241 ccctattagt aacaagatca gttctttgtt attcgttatt tttctattat gtttgttcat
      301 ttttatactc cttcttatat ttcctaatag agtataacat agctttaagc attacttaaa
      361 gataacttaa aagttatctt ttaaatactt aattgttaaa taccttattt gatgcaaaag
      421 aaatctacga tagtcaacaa aaaaattttc attatatcca gaaaaatttt tcttagttaa
      481 ggacctatgg attgcagtaa tgcagaattc gtcgaataaa ctgccaaaga catacaatca
      541 tattgctttt aaaattgacg aacaagatat agattcgttt gtctcaaaaa tccaaatgtt
      601 aggtttaact gtcgaaccag gacgttctcg tgtgaaaggc gaggcttctt caatttactt
      661 ttacgactat gataatcacc tcttcgagtt acataccggt actctccagg aacgtttaaa
      721 atcctacaat tccactacct gagactagta atgactagtc tctttttatg gcctctgatc
      781 cccccattga gcgaatacta atagggcttc gaattgatct gttcaatctt taagcaatat
      841 ccctcaaatg agtttggaaa gtttagtgat tcatcacaaa tacttaaata catgtagtta
      901 tatatagatt gaggttcttg tgagcgtatg ctcgtgtggg ttcaaatccc atcgtctgca
      961 tccatctttc caaaacaagc ggctaggaca tttttgtctc agccgcttgt tttctagttt
     1021 attctttcat cttgtcggac cttttttctt gggtatagac ttttctcata ttctaccaca
     1081 attttgggtt tccctaacac atgataggat ctcacaacaa attgctttct attattataa
     1141 taacctgtaa cgactaactt catcccttcg tctacttcag ctaggaagtt caaactatgg
     1201 gaagcaatca aacaattgac cccatttaaa ctgaaacgga tcaacggtct ggcactaact
     1261 tgcaaagtac ggatcttatc tactgttcca tattttttct ccataacctt cacctcaata
     1321 ccattatacg aacatacatt ccctttgtaa agaagaaact ctgttttatc tttttgactt
     1381 tttgtctatt acctttctta aaatcatatt ggcacttcct gcgaagatga aggacatgta
     1441 ctaaaaaaaa gaggcagcag tatagattac tgttgcttct ttactaattt aggtttactt
     1501 gttaagaaag attatttacg caaatgttct tcaacaattt gctgagattt atcagctgta
     1561 tcttccactt catcttttgc tttttctaac ttttgaatat attgtttcaa gcttgagttt
     1621 tcagcagttg cttcgtctaa tttcttttgt gtatctgtta actgagtttc tacatttgtt
     1681 ttttcttgtg tcagtgttgc gatttgttga ttcaaaccat ttacagtatt ttgtaattct
     1741 gaaagttttg cttgtgcttc ttgtagtctt gctgtccaat ctgcattcag atgattgatt
     1801 tcatcatttt tttggttgat ctcatctagt ttgacattga tttcattttg tttgctttgt
     1861 agttgtgcat ccttgctgac taattgactt tctaagtttt gtttttctgc tgccgattga
     1921 tttttttggt tttctaattc atttactttt gtttggtatt cttggacatc tgcctttgaa
     1981 tcttgcaatg tctgttcttt ttgtgctagt cgagcggtaa gatctgtaat cgcttgctct
     2041 ttatctgtca atgtatgatc aagctgtgtc agattctcat tgattgtatc aatatctgtc
     2101 tctccacccc acaaaataat ttcatttgcg taccgcgtgc ctgtgtagcc aattagacat
     2161 agaaatgcaa cgacaatccc accagtaata aatttctttt tcatttgcat agctccttaa
     2221 tccaatactt ttacatgcta aaagtaagtg atctcaaaca caatgtcaag cgtttttaaa
     2281 ttacatatgt aattttaata taaatactat caaatactca tttaatcctc cttttttatc
     2341 tatttaaaaa atctttttta tagagaaaaa tgcaggttaa tactttctta aataaatatt
     2401 tatggtgaac aaaaacaaaa aagcaagttt tattaaacgt aacccttttc atttcataga
     2461 ttactgtatt tattacaaaa aacg
//
LOCUS       DAZKKW010000180         2457 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.184, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000180 DAZKKW010000000
VERSION     DAZKKW010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2457
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.184"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            58..351
                     /locus_tag="ACG8PO_002840"
     CDS             58..351
                     /locus_tag="ACG8PO_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0208091.1"
                     /translation="MINLGEWGAIAGSITAIVSLILLVIKPITASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHAEKLVEHTQQIKTLFRERRK"
     gene            348..572
                     /locus_tag="ACG8PO_002841"
     CDS             348..572
                     /locus_tag="ACG8PO_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HFQ0208092.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGNK"
     gene            569..1594
                     /locus_tag="ACG8PO_002842"
     CDS             569..1594
                     /locus_tag="ACG8PO_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition family protein"
                     /protein_id="HFQ0208093.1"
                     /translation="MKKKITITAMSLLTALFLLPINTFAYTINDEYNLAPNQGDSRLA
                     IPNKIILHETGIDAPARNVAANMKNNYNGSNPYTTDVIGDGGIVYRVGEQGYVSWGAG
                     NANPYAPVQIELQRTYDKALFEKNYRAYIEYTRDSAKKYGIPLTLDQGTSLFTKGIIS
                     HLWVTNYVWGNHTDPYGYLSQMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGA
                     ANPTLTDGTNHAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADG
                     IYRPDVNQAYNTSGNVGYHVSFNMRNFPSKKVYVMMRATNDPEGNTKGGAQDFHDKRW
                     YLNIPKR"
     gene            1783..1856
                     /locus_tag="ACG8PO_002843"
     tRNA            1783..1856
                     /locus_tag="ACG8PO_002843"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1817..1819,aa:Ile,seq:cat)
BASE COUNT          818 a          409 c          479 g          751 t
ORIGIN      
        1 aagaagagag cgcatcttaa ttgatgtgct ttttattttg attcaaggag ttgtcacatg
       61 attaatttag gggaatgggg agcgatagca ggatcaataa ccgctatcgt ttctttgatt
      121 ttattagtaa taaaaccaat tactgcatct ttctcgaaga ttactgagac tctttcaaaa
      181 gtaagtcaca atttagattt gctgactaaa gatttagaat cgagcaaatc agatcgattg
      241 atgattcatg aagaactaaa gaaacacgat gaaagattag atacacatgc agaaaaattg
      301 gtagaacaca cgcaacaaat taaaacttta tttagggaga gaagaaaatg aataataaaa
      361 cgttcgaagt actaaaatgg ttcgcactgg taattattcc cgcactagct actttcgtgg
      421 ggttagttgg taaagcgctc aattggcagt acacagatat ctgtgttgtc atcattactg
      481 gttttggcgc gtttttaggg agtgtgttgg gtgtatcaaa tcgaacctac aaaatgttct
      541 cggctgaaag cgaagaagga ggaaacaaat gaaaaagaaa attactatta ctgcgatgag
      601 cctgttaacg gctctttttt tattacccat taatacgttt gcttatacta ttaatgacga
      661 gtataattta gcgccgaatc aaggagactc cagattagca attcctaaca aaattatttt
      721 gcacgaaact ggaatagatg caccagcaag aaacgtagcc gccaacatga aaaataatta
      781 taacggaagc aatccttata ctacagatgt tattggtgac ggtgggattg tttaccgtgt
      841 gggtgagcaa ggatatgttt cgtggggagc tggtaacgct aatccttatg cgcctgtaca
      901 gattgaatta cagcgcacat atgataaagc attgtttgaa aaaaactatc gagcttatat
      961 tgaatataca agagatagtg caaaaaaata tggaattcca ttgactcttg atcaaggaac
     1021 ttctttattt acaaaaggaa tcatttctca tttgtgggtg acaaattatg tttgggggaa
     1081 ccacacagat ccatatggtt acttatcgca aatgggagtt agcaaagaaa agcttgctta
     1141 tgatttagct catggattta ccgatgaaaa tccaacaact tctgaaaaca agcctgtcat
     1201 tgatccaaca cgagctggtg cagctaatcc tacgctgaca gatggaacaa atcacgccca
     1261 cattgatcag tttggggaaa tcgaaaacgc aaacttgcat gtggctggat ggcacattgc
     1321 taactataaa tacgagtata ttttcattat ggactacaat actgggaaag aattagctcg
     1381 agtaagagct gatggaattt atagaccaga tgtaaaccaa gcttataata cttctggaaa
     1441 tgttggttat catgtatctt tcaatatgcg taattttcct agtaagaaag tctatgtaat
     1501 gatgcgggca acgaatgatc cagagggaaa cactaaaggc ggtgcgcaag atttccatga
     1561 caaacgttgg tatttaaata ttcctaaacg ataaaaatag ctcctcgttg aggagcagta
     1621 cataactata ttgacaacta taaaaattat tcgataaaat agtgatgtta tcgcatatct
     1681 tcactatcac ccataatagt cacactccaa gctatgcgat aacaggtttg ttgccacaca
     1741 ttctactggt tgattgttta tggctttatg tggcaacaac cagtaccctt agctcagttg
     1801 gtcagagcag acggctcata accgtccggt cgtaggttcg agtcctacag ggtacattaa
     1861 cgtagccatt tgaatcgttc tgtgttagaa tttttttgaa gagtattata caagctaaag
     1921 cttttcttca ttgccactca aatgagtggc ttttttatgt atccttttat ggattaatga
     1981 aaggatgttt cacatagtta tacttctgta tatttgaaaa gttttacttt gatttttaaa
     2041 tagaaagaca tttgggttaa attgtgagat aataataaag aagagtttaa agcgcacccc
     2101 aaaccacttc cccataagtg tgttacgctt taaactcttt tatatttgaa gccattaaaa
     2161 agcataccat atttttgaaa aaaagtgaga aaaaaggctt acaattggag tggtagttaa
     2221 ttagtgactt atttttgatt ttatagcact gatactataa aatatagata tcatcatatt
     2281 acacaatctt aatactaact taaaaaatat ctcctttcac aagtatggtg ataaaattcg
     2341 ttccgggcta cctttttagg tagcctactt taatctttat acctttctgg atcaacgaaa
     2401 gtatacttta tatagtcata acgccgatga tcgctacgtg cgtccggcac gtcagtc
//
LOCUS       DAZKKW010000181         2406 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.185, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000181 DAZKKW010000000
VERSION     DAZKKW010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2406
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.185"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            80..>2406
                     /gene="esp"
                     /locus_tag="ACG8PO_002844"
     CDS             80..>2406
                     /gene="esp"
                     /locus_tag="ACG8PO_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HFQ0208094.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTT
                     ELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSISDVYNKYFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFTAG"
BASE COUNT          871 a          377 c          488 g          670 t
ORIGIN      
        1 ctattctgtg aataatacgt atcagattta tcttttattc ttactaggaa agtaattatt
       61 aagggagttg atttgaataa tggttagcaa gaataataag agagtatttc tagaaaaaac
      121 aaagaaacga gtattgaagt acagtatcaa aaaattaagt gttggggttg catctgtttt
      181 gattggtgtt ggtcttgtct tgggaacaac tgaactagtt aaagcacaag atgaaatttc
      241 tccaagcagc ccacttgaaa cagcgataag ctccgtacaa gtaggtgaca aagtagcttc
      301 ggggaatact tttcaggaga atcctggtta tacgaaaaat tataactttt cggatttaca
      361 atttagtcct caagaactaa ctggagacac actaaaaggc aatacaattg gatttgaagt
      421 ttacggaaaa cataatattg cagcatcaac taaaaactgg gaaatccgtc ttcaattaga
      481 tgaacgatta gctaaatatg tagaaaaaat tcaggtcgat ccaaagaaag gaattggatc
      541 ttctagacgg acctttgtaa gaattaatga ttcgctgggt agacctacaa atatttggaa
      601 ggtcaattat attcgtgcaa gtgatggatt atttgctggg gcagaaacaa ccgatacgca
      661 aaccgctcct aatggggtaa ttacgtttga aaagagccta gatgaaattt ttaaagagat
      721 aggcatagac aatctcaaga ccgatcgttt aatgtatcga atctacttgg taagtcatca
      781 agacgatgat aaaattgtac cgggtattga tagtacgggc tactttttaa cagattcaga
      841 tgatttctat aatagcttag atgtatctga aaataatcct gaccagttta aacatggatc
      901 agtaagtgca aaatatgaga aacctaatac tcaaacaaaa gatggttcag gatccacagg
      961 tgctaatgga gctataatct tagatcataa gttaacgaaa aattataatt tctcatattc
     1021 atcttccgca aagggcacgc cttggtatgc taattacaaa attgatgaac gattagttcc
     1081 atatgtagcg ggtatacaga tgcacatggt tcaggctgac aaagtaacat atgatgtttc
     1141 gtttgaatcc ggaacaaaag tagctgattt agcaatcgag cgacgggaag gtcatgaaaa
     1201 ttatggcata ggttcaataa ctaataatga tttaactaaa cttatcgatt ttgctaatgc
     1261 aagtccacgt ccagtcgtta ttcgatatgt tttacaattg actaagccat tagatgaaat
     1321 cttagaagat atgaaagcaa cagcacaagt tgaagaaaat aaaccatttg gtgaagattt
     1381 catctttgat tcttggttgt cggatacgaa taaaaaatta atccagaaca cttatggaac
     1441 aggttattat tatttgcaag atattgatgg tgatggaaac cctgacgata aagaagagag
     1501 cggagacacg aatccatata tcgggaaacc tgaattagaa gaagtatatg atgttgacac
     1561 aacagttaag gggaaagtat tcatccacga gttagcggga acaggtcaca aagcccaact
     1621 tgttgataaa gaaggtactg tattagcaga aaaaactatc gctccaaatg aaaaagatgg
     1681 ggctccaatt tcagatactg tagaatttga atttacgggt gtagattcaa gtaaattaat
     1741 cgcgaaagat gaattaaaaa tccaaatcgt ttctccaggt tttgataaac cagaagaagg
     1801 ttcaaccgtt attaaggaat caccaaaagc ggttgataaa caaaccgtgg tagttggatt
     1861 taaaccagat gctaaagaat caattcggaa taataaaaac ttacctgaag atgcagagta
     1921 ttcatggaaa acagagcctg atacttctaa cgttactgat agtacgaaag gtattgtaac
     1981 tgttaagatc ggaaatcgaa ctttcgacgt ggatgtagag tttgctgtaa aagcttctca
     2041 agctatggaa aatgatgcaa catacgtacc tataacaaca actccagaaa cgacagttca
     2101 aagtggaaaa cctacatttg ataaaccaga tgttccttta gctaaagatg ccttttcaat
     2161 ttcagatgtc tataataagt acttcggtaa tgcaagtgtt gatgcaaaca ctggtattgt
     2221 tacattcact ccagctaaag gtgtaggaga atcggagccg attactggaa caattcctat
     2281 taaaattgtt taccaagatg gttctgtagg cacgaccgat ttagcagtaa ctgtaagtaa
     2341 agatatttat gaaaatccag gagaaaacat tcctgcaggc taccacaaag taaccttcac
     2401 cgcagg
//
LOCUS       DAZKKW010000182         2366 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.186, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000182 DAZKKW010000000
VERSION     DAZKKW010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2366
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.186"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(240..539)
                     /locus_tag="ACG8PO_002845"
     CDS             complement(240..539)
                     /locus_tag="ACG8PO_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFQ0208095.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
     gene            890..1522
                     /locus_tag="ACG8PO_002846"
     CDS             890..1522
                     /locus_tag="ACG8PO_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFQ0208096.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
     gene            1645..2283
                     /locus_tag="ACG8PO_002847"
     CDS             1645..2283
                     /locus_tag="ACG8PO_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HFQ0208097.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
BASE COUNT          798 a          373 c          339 g          856 t
ORIGIN      
        1 ttaacttaca aaaatgcaac attaataaat aacgatagtt tcataaaata cacttttaat
       61 taacattatt agtttatact agctgttttt gtgttaatta aaggataaaa tacaatataa
      121 agaaacatct gtttctttat ttagaataaa aaaatacaga ctctgttcaa ataaatgaac
      181 ctagcctgca ttatgagatt gactatactt tttaattcat tgagatgaat aactcgtttt
      241 tataaatgcc caaaagtaat cggtgccgaa gccattcctc gacctaattc tttttcaaat
      301 tttcctattg ttgatatctc catataaaac ggtgatactg gtttagacaa ttctcccctg
      361 attgctaacg gacgtaaact acgttccaaa ttatttatga ctttaggata atactgacct
      421 ttttctaagc ctgtctttgc gtctatcaaa agtatacgtt ccttttcttt gatctctgga
      481 tttaaaacga gatcatataa ttttagtacc atgttttcta tttcttcact tttagacatc
      541 acgatttcct cccaaaaata tatttcttcc cactacattc tataaacaaa gtatacgcta
      601 catctagatt tttttaaatc attactggat tgttaaaacc tttcaataca ctgttatggc
      661 aacgattttt attttaaaaa cattctaaat taaatcagta aaaattttta ttctaagatt
      721 tttattagaa ttacgcctaa tttgtgtctc tcagggcaat tctaagaacg tttcaagaga
      781 tagcgacact atcgttatcg aaaaaggcga aaacctatct cattttctgc taatgtaagt
      841 tggtttagct tttgttaata tgactttatg aaaatggagg taattaaata tgatttttcc
      901 taaagttaat gaagcaagct tgaataaagg atcctttttt catatctatg aaaacgaaga
      961 ttttgaactg tataatatcc tttcacaaaa agaggtcatc gaaaaccaaa taatctgcca
     1021 tccaattatt tacaaatcta ttttcaagta tgcatttttt gcttccaatg attttcaaag
     1081 aatggagatt accgactgta tattcgaaaa ctgtgatttt tcaggctgta tattgacaaa
     1141 aagcatgttg catcgcgtga aatttgtcaa ttgtaaatta acaggtacta aattgatgga
     1201 aagttatatt gggaacacgt tattcgagaa ttgtaaaatg gactatgtaa atttatctgg
     1261 ttcaaacata aaagaaagta cctttgagca ctctgtctta tcaagcgcag attttgtaga
     1321 ctgttccctc actaaaataa tgttttctac taatgattta gaaaatgcta gcttttttga
     1381 tactgattta aaaaatatcg atttaagtag caactccttt gaaatgattg aagtacgctc
     1441 ggaacatcta aaaaattgta cgcttaatca gttgcaagca cttggttttg ctagaaaatt
     1501 tttacaaata aaagtagtat aatcaaaatg aatatataag ataaattttt agcaatacaa
     1561 atatccataa aaaaacattg cctattttaa ctcttcatgt atactgactc tatcttcaaa
     1621 aactgaatag taggagttat tatcatgaat aacattttag gaaaaaaaat agaacttaaa
     1681 aaagggatat tgggcactat tgtcgttgta ctcggtttgt tagggttgct atttgcgcca
     1741 tccattatta gcattttttc tttaactatc actggattta tagcatttac acttgttttt
     1801 ggtaaaaaag atacgaaaaa aatgttctcg aaacctgtcg ctcctgtcaa aaatatagcc
     1861 aaatattttt ttatcaatgt agtaatcagt gccgcagtct cggtattttt gcaacaaatt
     1921 ttgaaatggg gtttaacagg caatccaata aatgaggctt tcagcccgtt attatttatt
     1981 attttgccta tcatgatact tggagaagaa ttgttctcag tgtattttct cgctattttt
     2041 tctagtaagt tcagcattcc tgtcgctagt ttcttgtcag caatcatctt tggatttatc
     2101 cattacagta cttatgataa tggaaatatt ttacacactg tagcacacat ccttttgata
     2161 caaggggtgg caagattgtt attcaaccaa gctgccatta aaagcaactc gatcattaca
     2221 agttgggcag ttcatgtgat atttgatttc acagctatat tgctagtcgt tttattctcc
     2281 taaattttta tagaaataat acatgctcta ttgctaagaa agcatacttt atttccttta
     2341 aacaaaaaga cagatgtatc aatgga
//
LOCUS       DAZKKW010000183         2351 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.187, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000183 DAZKKW010000000
VERSION     DAZKKW010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2351
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.187"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..544
                     /locus_tag="ACG8PO_002848"
     CDS             <1..544
                     /locus_tag="ACG8PO_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFQ0208098.1"
                     /translation="ISKENLLNQDFSTETICEKWAADITYIPTKKNGWCYLSSIMDLH
                     TKRIISYTFSKRMTVDCVIQTLNKAKIHYHIPEGMILHTDLGSQYTAREVEQWLKTNK
                     IRHSYSRKGTPYDNAGIESFHASLKKEEVYTTSYSDFEEANRALFSYIEGFYNRNRIH
                     SSIHYLTPQEFEELAKEKMA"
     gene            complement(1017..1589)
                     /locus_tag="ACG8PO_002849"
     CDS             complement(1017..1589)
                     /locus_tag="ACG8PO_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0208099.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENCVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(1605..2210)
                     /gene="fic"
                     /locus_tag="ACG8PO_002850"
     CDS             complement(1605..2210)
                     /gene="fic"
                     /locus_tag="ACG8PO_002850"
                     /EC_number="2.7.7.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase Fic"
                     /protein_id="HFQ0208100.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
BASE COUNT          741 a          468 c          359 g          783 t
ORIGIN      
        1 catttcaaaa gagaatctct taaatcagga cttttctact gaaacgatct gtgagaaatg
       61 ggcagctgac attacgtaca ttcctactaa gaaaaatggt tggtgttact tatcttcaat
      121 catggattta cacacgaaaa gaattattag ttatacattt tcaaaacgaa tgactgtgga
      181 ttgtgtcatt caaacattga ataaagcaaa aatacattat cacattccag aaggaatgat
      241 tctacacact gacttgggca gccaatacac agcaagagaa gtggaacaat ggcttaaaac
      301 caacaaaata aggcattctt atagtagaaa aggaacacct tatgataatg ctggaatcga
      361 atctttccat gcctcattga aaaaagaaga agtctacacg actagctact cagattttga
      421 agaagcaaat cgagcgctat ttagctacat tgaaggattt tataaccgaa atcgaatcca
      481 tagctcgatt cactatctaa ccccacagga atttgaagaa ttagcaaaag aaaaaatggc
      541 ttaaccgtct ctactaaaat gtgtccaaga tattgactca aatccagtaa tatcacaata
      601 ggaatgctgt ttattatatt agcagcagtg ctgattttta tgatagttat ttggttctta
      661 aatgaaagat ctaaagacaa tcaacaccgt agggatatgg agataaaatc aatagagtca
      721 atagattatt tatcgataat aacactaaaa aagccacgtt cctaaacgtg acttacgctg
      781 aacgatatgt gttgtttggt agacgaacaa atcgttcatt ataaatatat gatggtatcc
      841 taatatagta agcgtggcta gcataccttt aattacttta ccttttacag ttaccataca
      901 cattttttga gcagaaatca agtaaataaa aaaatattat atttcacaac atgctaatag
      961 actcatatct agaacgcatt aagtatttac ctgtctggtt cgctgagact tcttttttaa
     1021 ctttcttcaa ttttcttctt tattctaaaa agcgtactgc gactgaaacc ggttatagct
     1081 tcaacttcct tatacgattt acctgaagta agcagttcat atgcgtgcct cttcttcggt
     1141 gtgattgttg ctttcggacg tccttctctg aaattaggat tattctttct tgcaaacatt
     1201 tttccttctt gggtacgttc cacaatcata tcacgttcca tttctgctac actcaacaac
     1261 gtccgtgtca ccattcgccc catggacgta ttttcaatcg ttcctagatt caaaaccttg
     1321 atcgttacgt tatcatcgag taaaggttca atgatctcta atgcttctct cgtgttacga
     1381 gcaaatcgat caagcttcgt aacaactatt tcatctccag gcttcagacc agccaacagc
     1441 tgattgaatt ttggccgatc ccgttttgta cccgtaaatt tttctgaaaa aatattcttc
     1501 ttctctacgc agttctcaag caactgttga atttgttctt ttgtactttg agaagcggtt
     1561 gagacacgtg catatccata tttcgtcatt tggtccacct acttttacaa atcatctatc
     1621 gtatactcag aatacccttc atagaaatag ctgacatcga ttcctttcat atataactcg
     1681 cgatcgtcaa ttttatcagt cagagcattg gaaattagat agcgtatttc caaatcgttt
     1741 atgggacttc gctccattgc agaaagatag tctgctttat cgattaagtt ccaatctaca
     1801 actttttgca attcttcctt taaaatgaga tccagccaaa ttctagtact tcggccattt
     1861 ccttctcgaa atgggtgagc gatattcatc tccacatatt tcttgataat ctcttcaaaa
     1921 gaagtttgtg gcatctgatc aatatgctgt aacgaatgtt ctaaatacat aactggtgca
     1981 aatcgaaaat ttccttttgc aatattgacc gatcggattt ttccggcaaa atcgtaaata
     2041 tccgaaaata gaaattcatg aatttcagat aaacctttaa atgtaccaac ttcgatttca
     2101 tttatttttc cacaatcgta caattctttc gctttttgtt tactgagttt ttcttcaacc
     2161 ttagccaact caacttgatt ggttaatccc aatttattct ctaaaatcat gcgaaatctc
     2221 cttaacttta tgacataagt ttatgaaaca ctctcaaccc cattatatcg cgattttgtt
     2281 tatgtgtcaa aaactattaa gtttctgacc cccgatagat ttttcaagtg tctctatttt
     2341 gcacagtcta g
//
LOCUS       DAZKKW010000184         2342 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.188, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000184 DAZKKW010000000
VERSION     DAZKKW010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2342
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.188"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            191..>2342
                     /gene="acm"
                     /locus_tag="ACG8PO_002851"
     CDS             191..>2342
                     /gene="acm"
                     /locus_tag="ACG8PO_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HFQ0208101.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLLIVTICAGIFLQK"
BASE COUNT          918 a          355 c          454 g          615 t
ORIGIN      
        1 ataatttaca ttaggaaaaa ccttgtaaaa acagtttttt ggcatattta ttttttataa
       61 atatttgtaa aataactatc aattttttag ttaaaagatt ggaaatgagt gggggaggaa
      121 ttgaagaaag gatttttgag agatgatata gtaggtttta tttttagtta aaatagggga
      181 gatagaaaaa atgaaaaagt gtagaaatat tattttatca atgttattta ttattactaa
      241 tataacatca ctgattcctg ttcatgtcta tgcggatgca ggcagagata tcagcagtaa
      301 tgtcacttcg ttgaccgtgg acccaacgaa tattacagat ggaggaaaca taaaagtaaa
      361 attctcgttt gacgaaaaaa aacaaaatat ccaaccaggt gattatcttt ggatcaattg
      421 gccaagtgaa ggaaatattc gcggtgaagg attccaaaaa gaaatcccat taatgattga
      481 aaataaaaat gttggaactt taacggtcag aaaagattct gcgcaggttg tgtttaatga
      541 aaatattaaa aacctagact cagtagaagg ttgggggcaa tttgagatac aggccagaaa
      601 cgtaaccgat accggtgaag aaaatacggg accattcacc gtgacgagcg gtgataaaac
      661 agctactgta aatgtgacaa agccagcttc tggttcttct tctagcgttt tttattataa
      721 aacaggagac atgctacctg aagatactaa gcacattcga tggtttttga atattaacaa
      781 taacggcact tatgttgaac aaccagttaa aatcagtgat gaaatccaaa gtggacaaag
      841 attagatcca agtacgtttg aaattaatca aatacactta ggtgaacaga aggtttatag
      901 aggagaagag ggaatccaac aatttttaca agatttcccc agcgctacat ttaatttttc
      961 agtaactgat aattacattg aaataactat accaaaaaac tttgtcaatc ttaggaaaat
     1021 aatggtgagt tataagacga tcatagaaaa cccagaacaa ataaattttg agaatcattc
     1081 ggaagcttgg tttaaagaat ttaataaacc tgctgttgat ggagaaagtt tcaatcatac
     1141 agttaaaaat atttcagctt ctggtggagt caacggtaca gttagaggcg aattgaagat
     1201 tttcaaatat atcaatgaca cagagattgg tatcccaaat gtgactttcg aattaagacg
     1261 agcagatgaa cagcctattc aaggacagtc tagtatcctg ttgacttcga atgaacaggg
     1321 agaagctagt atcaaaggcc tgcaagtagg agactatgtt gtaaaagaaa aagaagcacc
     1381 caattggatt gattttgatc cactaagttc aaatgaactc aaattctcca taaacgaaaa
     1441 tgatacagag ggagtcagtt tacctattta taacaagaaa aaggttacga acatcacagc
     1501 aactaaaatt tggaacggtg gaaccactcc aagaccgagt atttatttta aattattcag
     1561 agcaagtaca aacaattggg aaccagttcc tgatgcagaa acaaaacgat tagacaatgg
     1621 aataacttca gtgacttggg aagatatcca acagtatgat gatagtggaa acgaatatac
     1681 gtttaaagtg caagaagtag atcaaaacgg aaatgattat gtcccttcag gttatcaaaa
     1741 aattgaaaac gggctagtcg ttacaaatga gaataaagaa gtcatttcag tctcaggaca
     1801 aaaaacatgg gaagacaatg agaaccaaga cggcaaacga ccgacgacga tcacagtaaa
     1861 tctattagcc gacggtcaac cgatccaaca caaagaagta agtgaaaaag atgattggcg
     1921 ctatcgtttc acaaatctgc caaaatacag agacgggcaa gaaatcatct atactgtaac
     1981 ggaagacaac gtgcccgaat acagtacgac gattgacggg tataacatca aaaacagtta
     2041 tacaccggaa aaaacaaata tatctatacc aggtagccat ataacaccgt ggaaaaattt
     2101 tccagagaag acagagagag agaaaattag aaatctattc tctcaaaaat tgcaaatatt
     2161 tgctcataca ggagaatcca gcgaacaaag aagcaattat attagtaaga gagctgtacc
     2221 taaacagata acaaataaat caaaaagcat tcttcctaaa acaagtagta aaaaatctag
     2281 ttttgctgta atcattggtt tattgatagt aactatctgt gctgggatat tcttacaaaa
     2341 at
//
LOCUS       DAZKKW010000185         2334 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.189, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000185 DAZKKW010000000
VERSION     DAZKKW010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2334
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.189"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            122..736
                     /locus_tag="ACG8PO_002852"
     CDS             122..736
                     /locus_tag="ACG8PO_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0208102.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANAKDPQ
                     RRLVYKNIVEDLKKGVAIAKIAKDYNVTRQTVYRIKKDSMVNHE"
     gene            complement(760..1110)
                     /locus_tag="ACG8PO_002853"
     CDS             complement(760..1110)
                     /locus_tag="ACG8PO_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFQ0208103.1"
                     /translation="MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVV
                     TVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLTTIDYEARECIFLEKAHDSLVEE
                     LLMKVRTVFQKVSK"
     gene            complement(1104..1367)
                     /locus_tag="ACG8PO_002854"
     CDS             complement(1104..1367)
                     /locus_tag="ACG8PO_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PbsX family transcriptional regulator"
                     /protein_id="HFQ0208104.1"
                     /translation="MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQK
                     RLIIEAANIENELTIEQLFENYTDERNHVTIQNLGEAVGNEKW"
     gene            complement(1692..1871)
                     /locus_tag="ACG8PO_002855"
     CDS             complement(1692..1871)
                     /locus_tag="ACG8PO_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0208105.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESKIMCIALDLL
                     IAQEKAGFDIPALKK"
     gene            complement(1887..2087)
                     /locus_tag="ACG8PO_002856"
     CDS             complement(1887..2087)
                     /locus_tag="ACG8PO_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0208106.1"
                     /translation="MNKIIPLLFVLCIGEILMRHGAAVHLNFVVFLGILVFCLIFWRF
                     VLALVSLLFKLIAAIFFIGLFR"
     gene            complement(2130..>2334)
                     /locus_tag="ACG8PO_002857"
     CDS             complement(2130..>2334)
                     /locus_tag="ACG8PO_002857"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0208107.1"
                     /translation="TTIQKKSKMNLTVNECHINDLKGKKGNNHLYRKVRMCEIRTKTL
                     KLREWFLFRTNRSNRPRRLGGAK"
BASE COUNT          742 a          432 c          371 g          789 t
ORIGIN      
        1 cattagtttt tgtctatata aggtataccc taactagaca cgaccgcgac attgaaaaat
       61 ctggttttct cgtgtatgat tagggtgttt tttaaatagt tgcagaaagt aggtaaaaat
      121 tttgagagtt gcatacatac gtgtctcatc catcgatcaa aatgaacaac ggcagattga
      181 agaaatgaaa aaattcggtg cagagagaat tttcattgag aaacaatctg gcgcaacgat
      241 tactcatcga cctgtttttc aagaagcact ggatttcgtc cgagatcaag atatttttat
      301 tgtggaagct attgatcgac ttggaagaaa ttacgacgag atcatcgctt cagtcaatta
      361 cttgaagaag aaaaatgtgc agctgatcat cacaagtcta ccgatcatgg ccgaagcaat
      421 tggcaatcct ctcctggata aattcattaa agatttgatc attcaaattt tggccatgat
      481 cgctgaacaa gaacgtaccg aatctaaacg acgtcaggca caaggcatta agattgcaaa
      541 agcgaacggt gtttataaag gacgtcctaa gctatacagt gccaatgcaa aagatcccca
      601 acgtcgtctt gtttataaaa acatcgttga ggatctaaaa aaaggtgttg cgatcgctaa
      661 gattgcaaaa gactacaatg ttacgagaca aaccgtatat agaataaaaa aagacagcat
      721 ggtcaatcac gaatagttga tcatgctgct gtttctattt tatttgctta ctttttgaaa
      781 aactgttcta actttcatca ataactcctc aactaaacta tcatgcgctt tttccaaaaa
      841 aatacactct ctggcttcat aatcaattgt cgttagttga tctaataata cttttcctgt
      901 tgtcttcatt tcatattctg agtttatatt tacatatagc ggatagtttc tagttgtact
      961 agaaattgga gcaactgtaa caacgttact ataatccgca ataatatcat gacttaaaac
     1021 aatataaggt cttttacctg tctgttcgtg tccagatcga ggtgctgtat tcaataatag
     1081 aatatcgcct tgtctaggtt ttctcaccat ttttcattac ctactgcttc acctaaattt
     1141 tgaatcgtca catgatttct ttcatcagtg taattttcaa ataactgttc aattgttaat
     1201 tcattttcta tattagccgc ttcgataatt agccgcttct gaccgtttac ttcttcttgt
     1261 attatcttca ctttgtcttc tgtatgaatt ccaagatatt ccataatata ctttggcaat
     1321 ctaaggccat tactatttcc ccattttttt ataggtaaca tttccatagt agacacctct
     1381 ttctgtatat acatagtata acatacataa gaaaaaacgt atatacttaa ttatcctttt
     1441 ctattaaatt agattaacat tccatgaatc cataaactat gttaacattt atatgaaaat
     1501 agtcgaaagg aaatcataat atgtttaaga aaacaaaatc tttagaatca ggtaagaaaa
     1561 aaattgatct ggatacttta aagatttcat agttattgat acaaaatgga ataaaaagga
     1621 catagaaaat attctaaata gtaggaacaa ttaagtgcta tcacttttgt ttcttcaaat
     1681 gaaaagtgac gtcacttttt tagtgctggt atatcaaatc ctgctttttc ttgcgcgatc
     1741 aatagatcta acgcaataca cataatctta gattcacttt ttccgtactt ctgctttagt
     1801 tgctgcaatt ctttttgtct cttttcatct aatgtcacgg gtaaccgttt atttcctatt
     1861 gcaattggca ttagtactct ctcctttcat cgaaataacc ctatgaaaaa tatggctgca
     1921 atcaacttga atagtagact cacaagggct aacacaaagc gccaaaaaat caaacaaaat
     1981 actagtattc ctaagaatac tacgaagttt aaatgtacag ctgcaccatg acgcataagt
     2041 atctcgccaa tacacagtac gaacaacaga ggaatgattt tattcatatg gatctccttc
     2101 tttctcttag gttatcgggc tttgcccgct tatttcgcac cacctaagcg gcgggggcgg
     2161 ttggagcgat tggtgcgaaa taagaaccat tcccttaatt tcaaggtttt tgttcttatt
     2221 tcacacattc tcacttttcg gtataaatga ttattgcctt tttttccttt caaatcgttg
     2281 atatggcatt cgtttacggt caagttcatt ttggatttct tctgtatcgt cgta
//
LOCUS       DAZKKW010000186         2316 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.190, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000186 DAZKKW010000000
VERSION     DAZKKW010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2316
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.190"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            288..542
                     /locus_tag="ACG8PO_002858"
                     /pseudo
     CDS             288..542
                     /locus_tag="ACG8PO_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405927.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
     gene            612..1124
                     /locus_tag="ACG8PO_002859"
     CDS             612..1124
                     /locus_tag="ACG8PO_002859"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFQ0208108.1"
                     /translation="MTNKNNERYSTELKATAIKKMMPPENRGIRQLSQTLGIPEATLY
                     SWRKKARIQGNTTPGTKKNVEKWSSEDKFLIVMESFSMNQVELAEYCRKKGLFKEQIE
                     AWKKTCLSANEQEENRTRELATELKEEKKQARQLEKELRHKEKALAEAAALLLLRKKA
                     HAIWGDQEEE"
     gene            1121..2158
                     /locus_tag="ACG8PO_002860"
     CDS             1121..2158
                     /locus_tag="ACG8PO_002860"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFQ0208109.1"
                     /translation="MISSSDRKQAVELIQEANRNGARLTYACNELNISVRTYERWTRE
                     GTIAHDQRPLAKRPVPKNKLTDQEREKIIETVSKKEFMNLPPSQIVPKLADCSIYIAS
                     ESSFYRVLREKNMQHHRGRSQVSQKRIPPSHLATKPNEVWTWDITWLKGPIKGLFYRL
                     YLIIDLFSRKMVGWEIWESEEARHAEKLIKQTVLKERIQGQPLVLHSDNGSPMKAATF
                     QVLLEKLGIQSSYSRPRVSNDNPYSEAAFRTLKYRPEYPNNGFKTIDDARKWTKEFVD
                     WYNDSHLHSGIQFVSPAACHEGRHLAILEKRKQVYEDAKKRHPERWSKGVRNWTPHQS
                     VALNPLKETES"
BASE COUNT          842 a          419 c          465 g          590 t
ORIGIN      
        1 ataaattatc taaagagaat taagttttga ttatgtaata agttttggta ctaaaaaaga
       61 cttgatctaa ttagaccaag tcttttgtta gtgttatatt aataacaaaa taaaaataag
      121 aagtcgctca ctccccgacc aaagtttgtg agcaaaataa acagtcgatg tttgtttctt
      181 aattgtatat cttgatatta aacgatttaa gattattctt caagatatat attcgcaaat
      241 gtaattggaa aagcgacttc ttaaacaaaa ttaaggagtc gctttttatg gcaaaaataa
      301 cgaaaaatga gcaagaaaat ttggtggaaa attacacaaa aaaaccggcg tactctaata
      361 gccggttaga cgcacatttc gtgtgcactt ctaatccgaa attgagcttt gacgctatga
      421 cgatagtcgg aaaccttaat aaaaacagcg ctaaaaagct atccgatttt atgagtttag
      481 atccacaaat tagactttgg gatatacttc aaacaaaatt taaagctaaa gcactacaag
      541 accccctatg ttaggctagt tgtcgtatag gataataagt tataataaag actatacgga
      601 ggatgattac catgacaaat aaaaacaatg aacgttactc taccgaactg aaagcgacag
      661 ccatcaagaa aatgatgcca cccgaaaata gggggattcg ccaattaagt caaactttgg
      721 gcattccaga agcgactctt tattcttggc gtaaaaaagc tagaattcaa gggaatacta
      781 cacccggaac gaaaaaaaat gtcgaaaaat ggtcaagtga agataaattt ttaattgtga
      841 tggaatcatt ctcaatgaac caagtagaac ttgcggaata ttgccgaaag aagggcttat
      901 ttaaagaaca aattgaggct tggaagaaaa cgtgcttatc tgcgaatgaa caggaagaaa
      961 atcgaacgag agaacttgca actgaactaa aagaagagaa aaaacaagcg cgtcaattag
     1021 agaaagaatt acgtcataag gaaaaagcat tagcggaagc cgctgctctc ttattactaa
     1081 gaaaaaaagc acacgcgatt tggggggacc aagaggaaga atgatttcat cttcagatcg
     1141 caaacaagct gttgaactta ttcaagaagc caatcgcaat ggggcccgtc tcacctatgc
     1201 ctgtaatgag ctgaatatta gcgttcgaac ctacgaacga tggacgcgtg aaggtacgat
     1261 agcgcatgat caacgccctt tggcgaaacg tcctgtaccc aaaaataaac tgacggatca
     1321 agaacgcgaa aaaataatag aaaccgtttc aaaaaaagaa tttatgaatt tgccacctag
     1381 ccaaattgta ccaaaacttg cggattgctc gatttatatt gcatcagagt ctagcttcta
     1441 tcgtgtattg cgagaaaaaa atatgcaaca tcaccgtggc cgaagtcaag tttcacaaaa
     1501 acgcataccg ccaagtcacc ttgcgacgaa gccaaatgaa gtttggactt gggatattac
     1561 ttggctaaaa ggtccaataa aaggactctt ttaccggtta tatctcatca ttgatttatt
     1621 tagccgtaaa atggtaggct gggagatttg ggaatcagaa gaagctcgtc atgccgaaaa
     1681 acttattaaa cagacggtgc ttaaggaacg tatccaagga cagccgttgg ttttacactc
     1741 cgataatggc agccctatga aagccgcgac ttttcaagtc ttactcgaaa aattaggaat
     1801 tcaaagttct tattcaagac cacgagtgag taatgacaat ccttattcgg aagcggcttt
     1861 ccgaacgtta aaatatcgac cagaatatcc caataacggc tttaaaacaa tagatgatgc
     1921 tcgaaaatgg acgaaagagt ttgtcgattg gtataacgat tctcatttgc atagtgggat
     1981 tcaatttgtg tctcctgcag cgtgtcatga aggacgacat ctcgctattt tagagaaacg
     2041 aaagcaggtt tacgaggacg ctaaaaagcg ccatcctgaa cgctggtcaa aaggtgttcg
     2101 aaattggact ccacatcaat cggtggcgtt gaatccttta aaagaaacag aaagttaaca
     2161 ggagcataat ctttcaagat tatcaaataa ccctcgtcac ccaatagggg aaaaagttat
     2221 tcttccccta ttggataacg aggccaaccg accataggga ggttctgctt tttgataggt
     2281 ggaaaataaa aatacgacaa ctatattgac aaacac
//
LOCUS       DAZKKW010000187         2316 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.191, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000187 DAZKKW010000000
VERSION     DAZKKW010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2316
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.191"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            20..2173
                     /locus_tag="ACG8PO_002861"
     CDS             20..2173
                     /locus_tag="ACG8PO_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan D,D-transpeptidase FtsI family
                     protein"
                     /protein_id="HFQ0208110.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
BASE COUNT          792 a          414 c          519 g          591 t
ORIGIN      
        1 ataggcggaa ggataggaaa tgatgaaaga ttttatgaaa aaaatattaa atacttcttg
       61 gttagaaaaa ataagaaaag gtgcacaaga acctaagaac aaaacgtttc gccggtccca
      121 tgttcctttt cgcttaaatt ttcttttttt cattatattt acgttatttg ttgcgttgat
      181 tgctagattg ggttatttgc agatcgtgaa cggcgaagag atggaggcgc gagtgaagtc
      241 tacttctacg attacggtcc aagaaagtac tcctcgcgga atgatctacg actccacagg
      301 aaaagcttta gttacgaata aagcgaatca ggccatcaca tatacgagag gaacacaagt
      361 tacggctaaa gatttgcttg aaatcgcaac gaagctgaac tcgctgatcg atgttccggt
      421 agatgaaaat ctgacagata gagataaaaa agatttttgg ctggctgatc ccaaacattt
      481 gaaagaagcc acaaaaagac tttcggcaaa agaaaagcaa ttagggaaca gcgaacaata
      541 tagtgcgatg gtcaataaag tcacagctga tgagattaat ttaaacgagg agcaattaaa
      601 aattgctacc atctttaaac ggatgaatag tgcatatgaa ttaaacactg tctatatcaa
      661 aaactctggt gttactgatc aagagttagc tgttgtggca gaacgcgctt ctgaacttcc
      721 aggagtttcg actggcacag actggacacg agaatataat gcagcaagtt cactgaaaag
      781 tattcttggc agtgtgacga ctgaaaagca aggacttcca gcagatgaag cagagaaata
      841 cctagcaaaa ggttattctc ggaatgaccg tgtaggacaa agttatttgg aaaaacaata
      901 tgaagatgtc ctgcaaggaa ccaaaaccca atatgaagtt tcccttgata atgaaggaaa
      961 tgtttcgaat caaaaagaaa tcttctctgg ggaaaaaggt tctaatttaa tgttatctat
     1021 gaatgcagaa ttccaatcaa aagtagaaga aattttgaaa aggaattatc aaacattgat
     1081 caacaacggc aaagcacagt attcgcctgg tgcttatgca gtggctatga atcctcagac
     1141 aggagaagtg ctagcaatga ctggtttcag tcatgaacaa ggttccaaag aaatcaccga
     1201 gaatgcttta ggcacgatta ctagcgcctt tgatccaggg tctgtagtca aggctggtac
     1261 tttgacagca ggctgggcaa gtaacgcgat cagcggaaat caagttttga ttgatgagcc
     1321 gattcgtctg caaggtgctt cagaaaaaag ctctgtcttt aatcgaagcg gacaagttgc
     1381 tcttgatgca gtcaaagcat cggaattgtc ttctaatacg tatatgatca aagtggcgtt
     1441 aaagatgtta ggactagact atacacctgg aatgggacta cctagtttag atgaagaagc
     1501 taaagcttat cagcaattga gagactcttt caaagagttt ggtcttggta caacgactgg
     1561 gatcgatttg ccgaatgaat ctcctgggat ctcacgttct gttgattata tgaagaagtt
     1621 caatgcggat aatggcaaag aatggtatgc tccaggtaac tttaccgatt tggcatttgg
     1681 acagtttgac acatatacac caatccaatt agctcaatat gcttcaacag tcgctaatgg
     1741 cgggaaacga gtacagccgc acctagtcaa agcgatctat ggcaatgacg aaaatggaaa
     1801 cttgggtgaa gtgaagaaag aaattggcac aacagtagaa aatacggtca atatttctgc
     1861 agaaaatatg gcgatcctcc gtgaaggatt ccaccaagtt gtccatggta ccgatcctta
     1921 cacaacagcc aaaccattag ccagtgcgaa aatggacctt tccgcaaaaa cagggactgc
     1981 agaaacggtt gcggaaggtc atccagatat tgcgacagtc aatagtaata tcgtggcata
     2041 cggaccaaca gacaatccgg aaattgccat cagtgttgtc ttgccgaact tattagacga
     2101 acaagaccac ataaacttga cgattgctaa agaaataatg gacgcgtatt atgatatgtt
     2161 catggcaaaa taaaaaagag gtcgtgaaaa agccgcttag tttcgagtat gaagaaaaat
     2221 tttgtgaaaa tagcttctca catttttgct taaatttgac ttcataccga gaaactggct
     2281 tttgaacacc gtttactcgg caaaaaaggt tgtgac
//
LOCUS       DAZKKW010000188         2220 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.193, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000188 DAZKKW010000000
VERSION     DAZKKW010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2220
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.193"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(51..1598)
                     /locus_tag="ACG8PO_002862"
     CDS             complement(51..1598)
                     /locus_tag="ACG8PO_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HFQ0208111.1"
                     /translation="METTDTLLQLLQEAHKTNQAQQQTIQNLTTEIQLLNEKVNYLTN
                     KLFGRSKETLFEETNGQLNLFSDEEISVSVPEAAATIIPVKGHQRVVGTKADKIKHLP
                     ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCLTHYQHTYCCKSCEK
                     EGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQENYWKQTHGLDISRD
                     NITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESAKTDTYYWQFCTGKD
                     SQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESVRLVYCFAHVRRKFF
                     EAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQQELRPLFIAFYEWM
                     ATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERNIKELVIGRKNWLHS
                     TSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLSAETLDQLLPWNKDV
                     QQHFSRN"
     gene            complement(1700..2053)
                     /gene="tnpB"
                     /locus_tag="ACG8PO_002863"
     CDS             complement(1700..2053)
                     /gene="tnpB"
                     /locus_tag="ACG8PO_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFQ0208112.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
BASE COUNT          622 a          465 c          397 g          736 t
ORIGIN      
        1 tacgtcaaat aacccgacct atagaataga atcgggaatg tttggcgttt ttagttacga
       61 gaaaaatgtt gttgtacatc cttgttccaa ggcaacagtt gatctaaagt ttctgctgaa
      121 agtacgggaa gatttggtat cttatcaaat agaaactcta gatactttac aggattcaat
      181 ccgttaagtt ctgctgtttt atatacactc agaatgattc ctgaagttcg tgctccttca
      241 agacttgtgg aatgcaacca atttttccgt cctatcacca gctctttgat atttcgctcc
      301 gcacgattat ttgtgagttc taaacgacca tcttcaaaaa taggttcaaa accacttcgt
      361 agtttacacg catattgaac ggctgcatcc aacttggatt ttgcaacagg atctatcgtt
      421 gccatccact cataaaaagc aataaataat ggacgcagtt cttgttgccg acagcttaat
      481 cgcttctcag gagaaaagtc tttccatttc ttttctagga caaaccattt atccaattgt
      541 ttgactgctt gagctgcggg aatatccgtg tttttctttc cttttggaat tgcttcgaaa
      601 aatttccgcc gaacatgcgc gaaacaatag acaagtcgta cactttccac ggcattatac
      661 ccgctatagc cgtcacagtg taaataccct gtaaactcct tgagaaaagt tttcggaaca
      721 tctccagcac gactttcgtc gtggtgataa taaacgatgg gatgctgact gtcttttcct
      781 gtacaaaact gccagtaata ggtatctgtt ttggcacttt caatcacacg ataacttgtt
      841 tcatccccgt gtagaatctc ttcttgatta agatagactc ttaatcgttc tcctaaaggg
      901 tctaacgcgt tttgcactgc tttaatatgc caattggtga tattatctct cgaaatatcc
      961 aacccatgtg tttgtttcca atagttctct tgccgatagg caggaacttt ctgaccaaat
     1021 ttcatccgga tcgtttccgc cactaaacta ttggaaccta agctattaga aatcaaaggt
     1081 ttaggaacga tcgctttttt aaaagaagaa agtccttctt tttcgcagct tttacaacaa
     1141 taggtatgtt gataatgggt gagacaatcc aacatagcct gatggaaacg aatttcttct
     1201 cgtacttttg tccgtccgat atctttcatc tgtgaaccgc aatgttcaca aaattgttct
     1261 tccaaaggaa ggagatgttc tttctctgtt ataggtaagt gtttgatttt gtctgctttc
     1321 gttcctacaa ctcgttgatg tccttttact ggaataatgg tggcagctgc ttctggaaca
     1381 cttacagaaa tttcttcatc actaaaaaga ttcaattgcc cattcgtttc ctcaaagaga
     1441 gtctctttag agcgtccaaa taatttgttg gttaaataat tgaccttttc attcaatagt
     1501 tggatttcag ttgtaagatt ctgaatggtt tgttgttgtg cttgattggt tttatgggct
     1561 tcttgaagca actgaagcaa cgtatctgta gtttccaaaa tttttcacca ccttgaatcg
     1621 aactgatttt gttacagaat accacaagaa acgcacaaga aataggggtt caacacattt
     1681 tttgtgttaa ccccccagct taaaaaactc ctttttttcc tggcaaaata gctcgaggtt
     1741 gatcgatact taatccttct aacaaccatc gaacctgttg gttcgttaag gttcgaactt
     1801 cgtctttcgt ccgaggccat tttaacttgc cgttttctat ccgcttataa cagagaagaa
     1861 aaccatcacc gtcccaatag agaagtttga aacggtcttg acggttcccg caaaataaaa
     1921 aaacggcatc ttcgtaaagc tcgagttgat actcttgttg aataagactg gcaagaccat
     1981 caattccttt tcgcaaatct gtttttccgc acacaatata aatatgtttg gtttgaagta
     2041 aatcataaag cattggacaa cagttctttc aaaatagctt gaagaatata attgttaata
     2101 ccgttataga tcaaaacttc agaaccattc tctcttttga tgcgcatcgc taacttggca
     2161 gccgcgggtt tacctttttc tggtgcactc ttttcgtttc ctaattggac aggaacaatc
//
LOCUS       DAZKKW010000189         2200 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.194, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000189 DAZKKW010000000
VERSION     DAZKKW010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2200
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.194"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            41..1366
                     /locus_tag="ACG8PO_002864"
     CDS             41..1366
                     /locus_tag="ACG8PO_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFQ0208113.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRS
                     DNTGSGLILASSPEAKKRYGITNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTKSLNLFTTEETRSQRRKKLAQMIQERIKEELGLIA
                     TVGVGDNPLLAKLALDNEAKHNEGFIAEWTYENVPEKVWNIPEMTDFWGIGSRMKKRL
                     NQMGILSIRDLANWNPYTIKNRLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLNIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQINEEN
                     LDKIIDKIRQKYGFTSIVHASSMLESARSITRSTLVGGHAGGNGGIKND"
     gene            1359..1709
                     /locus_tag="ACG8PO_002865"
     CDS             1359..1709
                     /locus_tag="ACG8PO_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0208114.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEEIDADNEQRTSQNLAKEQMTTE
                     EIAEVLNAAVIKNRSVSIQKEERNAEGYYPEDIVGKILGSDELGIYVGKDKIHYDEIR
                     HIEFYQDNKWSRLE"
     gene            1706..1885
                     /locus_tag="ACG8PO_002866"
     CDS             1706..1885
                     /locus_tag="ACG8PO_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0208115.1"
                     /translation="MNIIQQYELKYITFDQLSEEIWGYGQLISMNPFLFLQIGVDTRI
                     MRVLNYYCKRNNKRK"
     gene            1897..2187
                     /locus_tag="ACG8PO_002867"
     CDS             1897..2187
                     /locus_tag="ACG8PO_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HFQ0208116.1"
                     /translation="MSINTFVYSHPINVYIIKNLGITVEQFCELYAYPQGTVASWITR
                     QRRIKSLPASFVYDLSLASSLNMSDVYEKLLSLEKEYDSFKIKHDKKIKKHI"
BASE COUNT          795 a          344 c          444 g          617 t
ORIGIN      
        1 aacatatgtt tgtataatgt ggaagaatgg agtgttattt atgatggatt attcaaaaga
       61 gccagtaaat gattactttt taatcgatat gaaaagcttt tacgcaagtg tcgaatgtat
      121 agaaagaaat ttagatccat taacaacaga acttgttgtt atgagtcgat ctgataatac
      181 tggttcagga ttgatattag cttcttctcc tgaagcaaaa aagcggtatg gtattacaaa
      241 tgtgagtaga ccacgtgact taccacaacc atttcctaaa acactacacg ttgttccacc
      301 acgtatgaac ctatatatca agcgaaatat gcaggtaaat aatattttca gaagatatgt
      361 ggctgatgaa gatctactga tttactcgat cgatgaatcg attttaaaag tgacaaaatc
      421 actgaatctt tttacgactg aagaaacacg aagtcagcga aggaagaagc tcgctcaaat
      481 gatccaagaa cgtattaaag aagagttagg tttgattgct acggtaggcg tcggcgataa
      541 tccccttttg gctaaattag ccttagacaa tgaagcaaag cataatgaag gatttattgc
      601 ggaatggacg tacgaaaacg ttcctgagaa agtatggaat attcccgaaa tgacggactt
      661 ttggggaatt ggttctcgaa tgaaaaaaag gctcaaccaa atgggaattt tgagcattag
      721 agaccttgct aactggaatc cttatacgat taagaataga ttaggtgtga ttggattaca
      781 gctgtacttc catgctaacg gaatcgatcg aacagatata gcaatcccgc cagaaccgac
      841 aaaggaaaaa tcttatggta attcacaagt gttaccacga gattatacta gaagaaatga
      901 aatcgagctg gtggtaaaag aaatggcgga acaagtagcc attcgtatca ggcagcataa
      961 ttgtaaaaca ggatgtgttc atttaaacat tggtacgtcg atcttagaaa cgagaccagg
     1021 attttcacac cagatgaaaa tacctatcac agataataca aaggaattgc agaattattg
     1081 tcttttcttg tttgataagt attatgaagg acaagaagta aggcacgttg gaataaccta
     1141 tagcaagctt gtgtacaccg attcgcttca gctggattta tttagtgatc cacaaaagca
     1201 aattaacgaa gaaaatttag ataaaattat tgacaagatt cggcaaaaat atggttttac
     1261 gtcgattgta catgcttctt caatgttaga aagcgctaga agcattacta gaagcacact
     1321 tgttggtggt catgcgggcg gaaatggggg aattaaaaat gattgattac aatgatcgtg
     1381 gacgtttaaa gtgggcaggg ttttatttat ctgatcatac tgaagaaatt gatgccgaca
     1441 atgaacaacg aaccagtcaa aatttagcaa aagaacaaat gacgactgaa gagattgcgg
     1501 aagtattgaa cgctgcagtc ataaaaaata gatcagtatc tattcaaaaa gaagaacgaa
     1561 acgctgaagg atactatcca gaagatattg ttggcaagat cttaggtagt gatgagctgg
     1621 ggatctacgt tggaaaagac aaaattcact acgacgaaat acgtcatatc gaattttacc
     1681 aggataataa atggagtcga ttagaatgaa cattatccag caatatgagc taaagtatat
     1741 aacctttgat cagctatcag aagagatttg gggttatggc caacttatct ctatgaatcc
     1801 ttttttgttt ttacaaatag gagtagatac tagaataatg cgtgtgttaa actattattg
     1861 caaacgcaat aataaaagaa agtaggctta cgcaccatgt ctatcaatac atttgtttac
     1921 tcgcacccga tcaatgttta cattatcaag aatttaggaa tcactgtaga acaattttgt
     1981 gagttatatg cctacccaca aggaacggtt gcaagctgga taacccgaca acgtagaata
     2041 aaatcattac ctgcttcatt cgtttatgat ttaagcctgg cctctagttt aaacatgtct
     2101 gatgtttatg aaaagctcct gtctttagaa aaagaatatg attccttcaa gattaaacat
     2161 gataaaaaaa taaaaaaaca tatataatat caatcacaat
//
LOCUS       DAZKKW010000190         2194 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.195, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000190 DAZKKW010000000
VERSION     DAZKKW010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2194
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.195"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            361..975
                     /locus_tag="ACG8PO_002868"
     CDS             361..975
                     /locus_tag="ACG8PO_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0208117.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDYVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVKLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSADAKDPQ
                     RRLVYKSIVEDLKNGVAIAKIAKDYNVTRQTVYRIKKDSMVNDK"
     gene            complement(1140..1964)
                     /locus_tag="ACG8PO_002869"
     CDS             complement(1140..1964)
                     /locus_tag="ACG8PO_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0208118.1"
                     /translation="MIERLKDFMISVWDVAMLADESNYTSSIYRLEDSNWIEISLELK
                     TLPNSVNYIIRDITSEAQQLVNYMHGHESIPFSLILLKRSKNISNTRDRFISIITACE
                     IGVKEFYQQEKPDLTLIFENLQSPPVDKLLGSMFKGYFNIEFPKEIRKEIKNFIEKRN
                     KLVHSPQGSKPTIEECFECYMAVLKTFNFFYKINDSFMYRDFYEESIELTPTEPNKSQ
                     ITLSKELERKVESQELAIGISITNVSTYKELYEAKSQDNKDFVENIDKEPTNTVGD"
     gene            complement(1988..2143)
                     /locus_tag="ACG8PO_002870"
     CDS             complement(1988..2143)
                     /locus_tag="ACG8PO_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0208119.1"
                     /translation="MEYKFNLNLKEVLPTQGTININGQDNDDDIVIKKIEFIDSDINV
                     FFILKKN"
BASE COUNT          746 a          364 c          310 g          774 t
ORIGIN      
        1 ttggttatta tctactacag tagttggttc atttgtacta tcagcaaatg taggaattgt
       61 agttccagtt acactaattc caatagttaa aatacctaaa gataaaataa acttattttt
      121 cattgtctat cacctcgaaa tcaaaactaa gaaaacgttt tcttaataaa tcttaacaga
      181 taaatcaagt tatatactag aaaattcata aaatatattt ttaatgtcat aatcgtttaa
      241 attttcttga ttgacattta tttacaaaaa atattgccat ttaaaaagaa aagtgacata
      301 tggcttcctc gtgtatgatt agggtgtatt ttaaatagtt gcagaaagta ggtaaaagtt
      361 ttgagagttg catacatacg tgtctcatcg atcgatcaaa atgaacaacg ccagattgaa
      421 gaaatgaaaa aattcggtgc agagagaatt ttcatcgaga aacaatctgg cgcaacgatt
      481 actcatcgac ctgttttcca agaagcatta gattacgtcc gagatcaaga tatttttatt
      541 gtggaagcta ttgatcgact tggaagaaat tatgacgaga tcatcgcttc agtcaattac
      601 ttgaagaaga aaaatgtgaa gctgatcatt actagtttac cgatcatggc tgaagcaatt
      661 ggcaatcctc tcctggataa attcattaag gatctgatca tccaaatttt ggccatgatc
      721 gctgaacaag aacggactga atcgaaacgt cgccaggcac aaggaattaa gatcgcaaaa
      781 gcaaacggcg tctacaaagg gcggcccaaa ttatacagtg cagacgcaaa agatccccaa
      841 cgtcgccttg tttacaaaag catcgttgaa gatcttaaaa atggtgttgc gattgctaag
      901 attgctaaag actacaatgt tacgagacaa accgtgtata gaataaaaaa agacagcatg
      961 gtcaatgaca aatagttgat catgctgctt ttattatctt ctcatcttta atttcctaca
     1021 tataattact atctataaaa ttaagtctta ctcagaacag ataaagcata aaattccaac
     1081 aaaccatttt ttacttaact aaataaagag tctgttaata taatcttcac ttatttacgt
     1141 taatccccta ctgtatttgt tggttcctta tctatatttt ctacaaaatc cttattatct
     1201 tgtgatttag cttcgtataa ttctttgtat gtcgacacat tggttatact aattcctata
     1261 gctagttctt gagactctac ttttctctct agctcctttg acaaagtgat ttgagattta
     1321 tttggttccg ttggagtcaa ttctatagat tcttcataaa aatctctata cataaagcta
     1381 tcattaattt tataaaaaaa attaaatgtt tttagcactg ccatataaca ttcaaaacat
     1441 tcttctatag ttggtttact tccttgagga gaatgaacca atttattacg tttttctatg
     1501 aaatttttaa tttcctttct tatttctttc ggaaactcaa tattaaagta ccctttaaac
     1561 atgctcccta ataatttgtc tacaggggga gattgtaaat tctcaaatat tagagttaaa
     1621 tcaggtttct cttgttgata aaattccttt acccctattt cacaggctgt aattatcgaa
     1681 ataaacctat ctctagtatt tgatatattt ttacttcttt taaggagtat aagagaaaag
     1741 gggatacttt cgtgtccatg catgtaatta actaattgtt gagcttcaga agttatatct
     1801 ctaattatat agttaacgga attaggaagt gtctttaact ctaatgaaat ttctatccaa
     1861 ttactatctt ctaatctgta tatgctcgaa gtataatttg attcgtcagc aagcatcgct
     1921 acatcccaaa cactgatcat aaaatctttt aaacgttcaa tcatttcagc aatattgcta
     1981 aaatcagcta atttttcttt agaataaaaa aaacatttat atcagaatca ataaattcta
     2041 ttttttttat tactatatcg tcatcattat cttgaccatt tatgtttatt gttccctgag
     2101 tggggagtac ttctttcaaa ttcaagttaa atttatattc cattacttct cctccaatat
     2161 ataaaaatta cttaactgta tccaaattta atat
//
LOCUS       DAZKKW010000191         2155 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.197, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000191 DAZKKW010000000
VERSION     DAZKKW010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2155
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.197"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..918)
                     /locus_tag="ACG8PO_002871"
     CDS             complement(<1..918)
                     /locus_tag="ACG8PO_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HFQ0208120.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
     gene            1044..2096
                     /locus_tag="ACG8PO_002872"
     CDS             1044..2096
                     /locus_tag="ACG8PO_002872"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HFQ0208121.1"
                     /translation="MIKCGENNEGGKYMQIDWNNLGFSYIKTPWRFIAKWKDGQWEEG
                     ELTEDNYLTIHEGSPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLM
                     PMVPEKMFVDAVKQVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVF
                     CTPVGAYFKGGLKPTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCV
                     YLDPATHTKIEEVGAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIE
                     GDIFIDDLDQFAEAGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGI
                     QFGDIEAPEGWIEVVE"
BASE COUNT          722 a          396 c          447 g          590 t
ORIGIN      
        1 ttttccgaca gcaattggac tgttcggaat cgtgaaacca gaagctgtca attctttgac
       61 ataggagctc aagaactctt cttttaaggc atattgtttt ccgttcaatg cgtagcagac
      121 cgtacgaatc gcaaaattgc tgtttccaag atccactaat ccaaagatag aaggttcagt
      181 ttggagctct tcttggtatt tttcagctaa tgtacgattt actcgatcaa tgatctcata
      241 gattttgtca tatccttctt caggaacaat ccgaatatct aaaagtactt gcatatttgc
      301 acgagagaga ttgctgatcg tggtgatgtt tcgattcggg atgaaatgga cagtaccatc
      361 cgttgctttc agctgaaggg tacggatacc aacagatacg acggttcctt caatcgttaa
      421 gtttgctagc ttgatgtagt ccccaacatc catctgttgt tccatgataa taaaaaatcc
      481 ggtaataaca tcactcatga acccttgggc accaagaccg attgccacac ctgcaattcc
      541 ggcaccagcc aaaagtgatc cgatcggcac accgatagca gataaaattg catagataaa
      601 aaagaaacca agggtataat gaaaaatagt atttaaaaga ctatgtaaag tctttaagcg
      661 gctctcgttt agaatggctt ttttagaata ctgattaaaa gacttatcaa tcagatattt
      721 tcccacacgg tgaagaatcc aaaaaaggac gatcaaaaaa atcagatata aacttttttg
      781 gataaaggca gccaaaatgg cgtcccaatc aatcccgtcc caaaaccgct gaaacgcatt
      841 tactttgctg acagcttgat ctgtcaatgt ctcgccagaa gagtctgtcg taccggcagt
      901 tgctaataat gaaaacataa aatcctcttt tctaaaattt gattctttct gtattgtacc
      961 aagaagtttt caaaaaagga aatagcaaaa ataactataa tgaaggaaaa aagctgaaaa
     1021 taaaatgaca aataatatgg ataatgataa aatgtggaga aaataacgaa ggtggtaagt
     1081 atatgcaaat cgattggaac aatctaggtt tttcttatat caaaactcct tggcgtttta
     1141 ttgccaaatg gaaagacgga caatgggaag aaggagaatt gacagaagat aattacctga
     1201 cgattcatga aggctctcca gcgcttcatt atggtcagca atgttttgaa ggtctaaaag
     1261 cttatcgtcg aaaagacggc aaggtcaatc tctttcgacc tgaacaaaat agtcgacgga
     1321 tgaatcaaag tgctagacga ttgctgatgc caatggtccc tgaaaagatg ttcgttgatg
     1381 ctgtcaagca agtggtaaaa gcaaatgaag attttgtgcc gccctatgga acaggcgcta
     1441 cattatactt gcgcccttta ctgattgggg tcggtgaaaa cattggtgtc catccagctc
     1501 cagaatattt atttgtcgtc ttctgtacgc cagtaggagc gtatttcaaa ggtggactaa
     1561 aaccaacgaa ttttattgta tctgattacg atcgtgctgc tcctaagggg actggtgcag
     1621 caaaagtcgg cggaaattac gcagcgagtt tacttccagg aacagaagca aaagaactgg
     1681 attactcaga ttgtgtttat cttgatccag ccacacatac gaaaatcgaa gaagtaggtg
     1741 cagcaaattt ctttggaatt acaaaagacg gaaaattcat cactccgcaa tcgccctcta
     1801 tcttaccaag tatcacgaaa tattccttgt tgcagttagc tgaagaacga ctaggactgc
     1861 aagcaattga aggagatatt ttcattgatg atctagatca atttgcagaa gctggtgcat
     1921 gtggtacagc ggctgttatt tctccaatcg gtgggattta tcacaataac aatcttcatg
     1981 tattttatag tgaaacggaa gtaggaccag taacaaaacg gttgtatgat gaattgacag
     2041 gtattcaatt tggagacata gaagcaccag aaggctggat tgaagtcgtg gaataatttc
     2101 aaagtaatga gttgaaaaaa acgtaaaaag aaaaaatcag gaagagactt taaaa
//
LOCUS       DAZKKW010000192         2125 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.198, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000192 DAZKKW010000000
VERSION     DAZKKW010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2125
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.198"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..159
                     /locus_tag="ACG8PO_002873"
     CDS             <1..159
                     /locus_tag="ACG8PO_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HFQ0208122.1"
                     /translation="RRNKFYRSLRTASSTIKGMETIRGIYKKNRRNGTLFGFSVSTEI
                     KVLMGIPA"
     gene            242..859
                     /locus_tag="ACG8PO_002874"
     CDS             242..859
                     /locus_tag="ACG8PO_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011834674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0208123.1"
                     /translation="MWIFKQEIFAKLFSLDGLGWGILFFIFLFLGSMWIWFVDKKNSY
                     EKVIVKKMNRNEFDDFSKEHRVELYDSHEINSTSPSYYWVFGCEEKPLEIKLLKMLEN
                     PPVIEDFSAKNVEKQMFEQVQIRGQNKISLEPDEYAAFFLETNSGTSMPVFKVKLKMN
                     GRLCTYDLVANMRYGSTGDRRTILKFEQTFRSFLKNRVSQIFNLD"
     gene            1023..1355
                     /locus_tag="ACG8PO_002875"
     CDS             1023..1355
                     /locus_tag="ACG8PO_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0208124.1"
                     /translation="MNNREILAKEMADLKAKEAKIRAKRKQIRQKEMISLAKLYCKKE
                     GFKTPAEALDFLESNKRNHSVNDEVLVSLRKFADGLVWQENGAYWRTNDVKGLTQFMA
                     QFRSENVN"
     gene            1679..1840
                     /locus_tag="ACG8PO_002876"
     CDS             1679..1840
                     /locus_tag="ACG8PO_002876"
                     /inference="COORDINATES: protein motif:HMM:NF013258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="HFQ0208125.1"
                     /translation="MAYMVARTQKHKVGSLNGLQNHVDRKTKNHTNKDIDITKSGSVA
                     KFSKKSILV"
     gene            1872..2125
                     /locus_tag="ACG8PO_002877"
                     /pseudo
     CDS             1872..2125
                     /locus_tag="ACG8PO_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
BASE COUNT          758 a          272 c          429 g          666 t
ORIGIN      
        1 cgacggaata aattttatcg aagtctccgg actgcctcat ccacgattaa ggggatggag
       61 accattcgag gaatatataa aaagaaccga agaaatggaa cgctcttcgg cttttcggtg
      121 tctactgaga tcaaggtatt aatgggaata ccagcctaag atatttggag ttcagagagg
      181 gcgcgtttga ttttcaaact tcgcaacaga accattattt taaagtggga ggaaaattgt
      241 tttgtggata tttaaacaag aaatctttgc taaattattt agtttagatg gattaggttg
      301 gggaatctta ttttttattt ttttattttt aggatctatg tggatctggt ttgttgataa
      361 aaaaaattct tatgagaaag taatagtgaa aaaaatgaat cgaaatgaat tcgatgattt
      421 ttcaaaggaa catagagttg aattgtacga tagtcatgaa attaacagta cttctccatc
      481 ttattattgg gtatttggtt gcgaagaaaa accattggag attaaattgt tgaaaatgct
      541 tgaaaatcct ccagtcattg aagatttttc tgcaaaaaac gttgaaaaac agatgtttga
      601 acaggtacaa ataagaggtc aaaacaaaat ttcattagaa ccagatgaat atgcagcctt
      661 ttttttagaa acaaattcag gtacttcaat gcctgttttc aaagttaaat taaaaatgaa
      721 tggtcgttta tgcacttatg atcttgttgc aaatatgcgc tatggttcta ctggtgatag
      781 acgtactatt ttaaaatttg agcaaacctt tagaagtttt ttgaaaaata gagtatccca
      841 aatttttaat ttggattgaa aaaattaaaa atttggccaa aaatactaca gttttttaga
      901 gcaatcatct tatgatgatt gctctttttt tgttgttttt gtgtcattcg ttgttcaaat
      961 cctctattcg taaaataaat attttaaact agaaatatgc cttattttta ggaggaatca
     1021 aaatgaacaa tagagaaata ttggcaaaag aaatggcgga tttgaaagca aaagaagcga
     1081 agattagagc gaaacgaaaa cagattagac agaaagaaat gatttcttta gcgaaattat
     1141 attgtaaaaa ggaaggcttt aagacacctg ctgaagcatt agattttttg gaatcaaata
     1201 aaagaaatca ttcagtaaat gatgaggttt tagtatcgct aagaaaattt gcagatggat
     1261 tagtttggca agaaaatggt gcatattggc gtacgaatga tgttaagggt ttgactcaat
     1321 ttatggcaca atttcgttca gaaaatgtga actaaattga ggaggggttg aagtgaaaag
     1381 caatactttt cacttttggt tttttgcgga ataaaaattg tgaaaacaat gacgcaaaaa
     1441 gccaatggtt ttgaacgaaa aaaggacgta aaaaaggaat gcgaaggcag tcgtttttcg
     1501 ttcgcttttt cgtttgagag aaacggagtt tctcatcggc tgttaagccg atatagccct
     1561 cggcagagct tacagacttt acgcagtata agtctagtaa gttatacctt acatggaggt
     1621 ttttccgaat ttagcttgtg attctcgcaa taattttaac gaaaggaagt acacagttat
     1681 ggcctatatg gtggcacgaa cacaaaagca taaagttggg tcgttaaatg gacttcaaaa
     1741 tcatgtagat cgtaaaacga aaaatcatac gaataaagac attgatatta cgaaaagtgg
     1801 ttctgttgca aagttttcca aaaaatctat tttagtgtaa aattgagaaa aaagacagag
     1861 aggatagagt aatgaatcat tttaaaggca aacaattcaa aaaatgacgt cattattgtc
     1921 gctgttagtt actacctgcg ttacaatcta agctatcgtg aagttcagga attgttatat
     1981 gatcgtggaa taaatgtttg tcatactacg atttatcgtt gggtgcaaga gtacagcaaa
     2041 gtcctcgatt atctttggaa gaagaaaaat agacaatcct tctattcatg gaaaatggac
     2101 gaaacctata tcaaaattaa gggac
//
LOCUS       DAZKKW010000193         2114 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.199, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000193 DAZKKW010000000
VERSION     DAZKKW010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2114
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.199"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..845
                     /locus_tag="ACG8PO_002878"
     CDS             <1..845
                     /locus_tag="ACG8PO_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage replisome organizer N-terminal
                     domain-containing protein"
                     /protein_id="HFQ0208126.1"
                     /translation="NKRYYYLKLKENFFDSDEMVLLESMPDGYIYSNILLKLYLRSLK
                     HEGKLMFNDRIPFNSTMLATITRHSVGVVEKAVQIFRDLQLIEVLDNGAIYMSDIQSF
                     IGKSSTEADRKRKYRKEIEEAKQNLITSGQMSDKCPDKTTPELEIELEKDIELEKDIE
                     NVTPSKKSKAKPVRHKYGEYKNVLLSDEQMEKLKIEFPNDYQERIERLSEYCESSGKT
                     YKNYLATIRSWARKEKSESKNASSGYKRTGRREKLPEWAIDQEAYLKKKALERANRQS
                     KAPF"
     gene            857..1126
                     /locus_tag="ACG8PO_002879"
     CDS             857..1126
                     /locus_tag="ACG8PO_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0208127.1"
                     /translation="MKIDYLELINEIAKYKTGEEIEILRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDTTQLRNLAYTKLLFVDCVKGLQKHLNELEQV"
     gene            1131..1295
                     /locus_tag="ACG8PO_002880"
     CDS             1131..1295
                     /locus_tag="ACG8PO_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0208128.1"
                     /translation="MDLKTFTAQIELMHQEALRQSVSYEDKWLNTFHGGRESALDQVL
                     KLLKGECRDG"
     gene            1288..1590
                     /locus_tag="ACG8PO_002881"
     CDS             1288..1590
                     /locus_tag="ACG8PO_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0208129.1"
                     /translation="MDKKAAMQRIIELTYSEDWQNDKEAASEVMRLGRAMWADKSNKP
                     RPRKIAIWHGDKLLVIGTAEQLASLTGLHEKIVRKRARCGYTDAKKRTFRYVEESS"
     gene            1587..1748
                     /locus_tag="ACG8PO_002882"
     CDS             1587..1748
                     /locus_tag="ACG8PO_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFQ0208130.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIHRLEE"
     gene            1745..2050
                     /locus_tag="ACG8PO_002883"
     CDS             1745..2050
                     /locus_tag="ACG8PO_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0208131.1"
                     /translation="MSVDKKAAMKRIAELTKSESWQEDKEIVAEVQKLGKSMWTEKPK
                     RRTPRKIAIWHDDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEER"
BASE COUNT          822 a          327 c          484 g          481 t
ORIGIN      
        1 acaacaaacg atactactat ttaaaactaa aagagaactt tttcgatagc gacgagatgg
       61 ttcttttaga aagtatgcca gatggataca tttattctaa tattcttctc aaactttatt
      121 taagaagtct gaaacatgaa ggcaaactaa tgtttaatga caggattcca ttcaattcta
      181 caatgcttgc gactattaca agacactctg tgggagttgt agaaaaagca gtacaaatat
      241 ttcgtgattt acaacttatt gaggtattag ataacggagc aatttatatg tctgatatac
      301 aaagttttat tgggaaatct tcaactgaag ctgataggaa gagaaaatac agaaaagaaa
      361 ttgaggaagc aaaacagaat ttaataacaa gtggacaaat gtcggacaaa tgtccggaca
      421 aaactacacc agagttagag atagagttag agaaagatat agagttagag aaagatatag
      481 agaatgtaac gccttcgaaa aaatcgaagg ctaagcccgt ccgtcataaa tacggagagt
      541 ataaaaatgt tcttttgtca gatgagcaga tggagaaact caaaatagaa ttccctaatg
      601 actaccaaga gcgaatagaa cggctatctg agtattgtga atcatctggt aagacttata
      661 aaaactattt ggcaactatt cgaagctggg caagaaaaga aaaaagtgaa tctaagaacg
      721 caagcagtgg atacaagcgc acaggaagac gagagaagct tcctgagtgg gcaatcgacc
      781 aagaagccta tctcaagaaa aaagcgctag aacgagctaa tagacaatca aaagcaccat
      841 tctaagaggt gggaaattga aaatcgatta tctagaacta attaatgaaa tagcaaagta
      901 taaaactggt gaggaaatag aaatcctgag agacgtatat gatcaactcg aagaagctgg
      961 aatcgaagga attaagaatg atcgttcgag ttggagtaag ctcagatact atttcgcact
     1021 ttatatcgat acaacacaat taagaaattt agcatatacc aaattactat ttgttgattg
     1081 tgttaaaggc ttgcaaaaac atcttaatga acttgagcag gtgtaatcag atggacctaa
     1141 agacatttac agcacagatt gaactaatgc atcaagaagc tttaagacaa agtgtatcgt
     1201 acgaagacaa gtggctcaac acgttccacg gcggacgtga gagcgcactt gatcaagtac
     1261 tcaaattact gaaaggagaa tgtcgggatg gataagaaag cggcaatgca gcgaattatc
     1321 gaattgactt attcagaaga ttggcaaaat gacaaagaag ctgcttcaga agtgatgagg
     1381 cttggaagag cgatgtgggc agacaagagc aacaagccaa gaccacgaaa aatcgcaatt
     1441 tggcacggtg acaaacttct agtgataggg acagctgaac agttagcaag tctcacaggc
     1501 ttgcacgaga aaatcgtgag gaaaagagca aggtgtggct acacagacgc taagaagaga
     1561 acgtttagat acgtggagga atcatcatga caacagaaga agtgattcaa atgcgtattc
     1621 gaagccttca gcgtgagatt gacgatctgg aacgaacaaa ggcagtgatg gtcaatgaaa
     1681 cggcgagaaa ggcaatcgat ttgcacatag agaatttaag aagggaaatc catcgattgg
     1741 aggaatgagc gtggataaga aagcagcaat gaaaagaatt gctgaattaa ccaagtcaga
     1801 atcttggcaa gaagacaaag aaatagttgc agaagtccaa aagctcggta aatcaatgtg
     1861 gactgaaaag cccaaacgga gaacgccgag aaaaattgca atctggcatg atgaccgaat
     1921 tttagtaaca ggtactgctg aacagttatc tgaaattact ggattaagca aaaacattat
     1981 ctgggataga gctaggagct tatggattga ttcaaaagga cgacagttta ggtatgtgga
     2041 ggagagataa tggatctcat tacacaatac agtgacatca tcctcaagaa aatcatgatg
     2101 aagattcaga aaga
//
LOCUS       DAZKKW010000194         2096 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.200, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000194 DAZKKW010000000
VERSION     DAZKKW010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2096
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.200"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(49..1236)
                     /locus_tag="ACG8PO_002884"
     CDS             complement(49..1236)
                     /locus_tag="ACG8PO_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit R"
                     /protein_id="HFQ0208132.1"
                     /translation="MQNTAHKNPYKTVDEATQLDLVIVVNQLLTGYDSKWVNTLYLDK
                     SLEYQNIIQAFSRTNRLNGPEKPFGTIRYYRKAHTMEQNIEEAIELYSGKQAKGLFVE
                     KLYNNLEKLNDIFEEIKSVFNISGIENFSHLPEVPGEIKQFVNLYNQLIHCYESAMIQ
                     GFTWAKSTYEIQDYEPITVVITEYEFNILAQRYSEIEREPRDGNDSIAYDIDYQLPEG
                     SRSRINYEYLNTNFKKYMNIKEERDSNLIQSLLNDLHRTFAQLPVELQPYAEMILNDV
                     NNGELVIKEGWEFTDYLNEYQLSEEDDFIQDLVDSTNINEALLREMLDLRLTEVNINE
                     YSRFDKLKSSVNVGHFSGYIEKNLGKMVIPIKVNMLIDRLLRAFLLEDVFDVTEYIKN
                     YFN"
     gene            1272..2045
                     /locus_tag="ACG8PO_002885"
                     /pseudo
     CDS             1272..2045
                     /locus_tag="ACG8PO_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          638 a          410 c          340 g          708 t
ORIGIN      
        1 aactaaaaaa gatgaatttt tttatgtact ataagatgta ggtgtactct agttaaagta
       61 attctttatg tactctgtaa catcgaatac atcttctaat aaaaatgcac gcaataatct
      121 gtcaattagc atatttacct tgattggtat taccatttta cctaaatttt tctcgatata
      181 tccactgaaa tgacctacgt taacacttga ttttaattta tcaaatcgac tatattcatt
      241 aatgtttacc tcggttaagc gtaaatccaa catctcacgt aataatgcct cattaatatt
      301 ggttgaatcc actaaatctt ggataaaatc gtcctcttca ctaagctgat attcattgag
      361 gtaatcagta aattcccatc cttctttgat aactaattcc ccattattta catcatttag
      421 aatcatctca gcatatggct gtaactccac tggtaattgc gcaaatgtgc gatgtaagtc
      481 gtttaacaat gattgaatta aattcgaatc acgttcttct tttatattca tgtatttttt
      541 gaagtttgtg tttaaatact cataattaat acgactacga ctaccttcag gtaattggta
      601 atcaatgtca tacgctatac tatcatttcc gtctctaggt tctctctcta tctcgctgta
      661 acgttgtgcc aagatattaa attcatattc tgtgattact actgttatag gttcataatc
      721 ctggatttca taagtcgatt ttgcccatgt gaatccctgt atcatcgctg attcataaca
      781 atggattagt tgattataaa gatttacaaa ttgcttaatt tcccctggaa cctccggtaa
      841 atgactgaag ttttctatgc cagaaatatt aaatacggac ttaatttctt cgaatatatc
      901 gttcaacttt tctaagttat tatataattt ttcaacaaat aacccttttg cttgtttgcc
      961 tgagtataac tcaatagcct cttcaatgtt ctgttccatc gtatgtgctt tacgataata
     1021 acgaatggtt ccgaacggtt tttcaggacc attaagacga tttgtgcgtg aaaaagcctg
     1081 aattatgttc tgatattcaa gtgacttatc taagtatagg gtgttgaccc attttgaatc
     1141 gtaacccgtt aagagctgat taacaactat aacaagatct aattgggttg cttcatctac
     1201 tgttttgtaa ggattcttat gcgctgtgtt ttgcaatagt tttgtacaca agattgcaaa
     1261 gaaaagtaca caatgttgcc acttctggtt ccatgagttt tatttgtgtt gatgggacaa
     1321 ccaatcagct atttacgacc cttgaagatc gcagacttta caaactaact caatacttcc
     1381 tgcgctttcc tagaaaggcc cgtttaaagg tcaagtacct agtgatggac atgaatgcca
     1441 gctattgtca gttgcttaaa acggtctttc caaatgcgga gatcgtaact gaccgctttc
     1501 atattgtcca acacatcaat cgcagcttca atcaattacg tgtccagatc atgaatcgtt
     1561 ttcgaacctc acactctgaa aatcagaaga agtaccgtcg gctcaagcga tattggaaac
     1621 tcttactcaa agattccacg acatgggagg cactcaaacg gcattatcat cgactgttta
     1681 aacgaccaat tagtcaaaca gaaatcgtta atgagctgtt aagctacaac gaagaattgc
     1741 gaactgcgta tcatttttgt caattattac gctattattt cgttaaacga gatacggtag
     1801 gctttcaaga aacccttaaa acaatttcat ctacgttacc tcagtcgttt aaaaagaaat
     1861 tcacgatctt ccatcgctac caatcgggta tttcaaatgc ctttaaggtg tcccactcaa
     1921 acggcgtcac tgaggggctg tataacaaga tcaagctgat taaacgaatt gcgtttggtt
     1981 atcgcaactt ttataacttt cgggcacgga tctatttaca acaaggtctg atctttgaaa
     2041 actaaaaaaa tatgcggcgc acagaacgcg ccgcatatcg gactaagttt tattgg
//
LOCUS       DAZKKW010000195         2082 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.201, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000195 DAZKKW010000000
VERSION     DAZKKW010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2082
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.201"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(100..707)
                     /locus_tag="ACG8PO_002886"
                     /pseudo
     CDS             complement(100..707)
                     /locus_tag="ACG8PO_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313657.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase family
                     protein"
     gene            1230..1904
                     /locus_tag="ACG8PO_002887"
                     /pseudo
     CDS             1230..1904
                     /locus_tag="ACG8PO_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
     gene            1897..2058
                     /locus_tag="ACG8PO_002888"
     CDS             1897..2058
                     /locus_tag="ACG8PO_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0208133.1"
                     /translation="MTEKEQVQQIVKKYNKSIADLSENASAKEFKTVMNYVADEANRK
                     QRKLVGLDK"
BASE COUNT          748 a          330 c          357 g          647 t
ORIGIN      
        1 atgaatataa atagagtgta tcttctttga taaatgtcag aaaaagtgtg acaaataacc
       61 atcagtaaaa ttacttgtca tactattgat aaggagtatt taattgaaga gaatgattag
      121 gagaaaggat ctccgtactt tttatagtaa tcatagaatc caagtttgta tagacgaatc
      181 tttttttctc ttatttcttc tgtttcaaaa tggactgctt gaagaatcaa gtctgtgaat
      241 tctataggag ctgttccatt cgcagtaatc aaattacgat ctttcacagc ttgttgttct
      301 ataaaatctt ctttattctg ataattttcc gattctaacc aaggaaattg cgcatttccg
      361 gtgtgtttgt aaccttgcaa taatccgctc tgagctaaat aatcaactgc cccacaaata
      421 gcagctactg gtacgtctgt ttttaacaat ttatcgatta tttgatataa atgatgattt
      481 tttatgtccc atgaattgcc accgatcata ataaaaaggt caactttttc aggtatttgt
      541 tcgattaagt aatcgatttt tgtttttaat cctccaatgg aagtgacctc tgattggata
      601 gaagcagttt tgttttgcca tccctctttt tggtttagtg tgctagtaat ataagcacct
      661 tcccaatcgg catattcctc taatagaaac gtaatcgcag ttttcatttt caagctctcc
      721 tttactatct atgacaagga caacagtttt aacatacaga cattcattaa atttggatag
      781 cgacagcgaa aacaacaatt cttaagtttt agacagagta tacagacagt caacttaatt
      841 aaatacgaac taaataaaaa tgacaggtaa tcatttttgt ttaccagcat tataacattt
      901 tgcatacatg gatgacaaaa gaaccaattc ccacttgctt gagtacaatc ttaaaagaaa
      961 ctggaaggca aaaagtaatt atagaaacga aaaataaatc atatcgggca ctcagacatg
     1021 aaaacaaccc tagaaatata taactagtcc aatgaaaaaa aagcgaatgc cgtagaaaac
     1081 aaggattctg cgtacattca tatctcattt tgcgagcata tctgctatta ctgtgactaa
     1141 atgttaatat ggttaaaatg accgttatat tttttatata aatatcgtat aattagtatg
     1201 aatatagaag aaggaaagaa ggtgcaatct tggaacctaa agacaagtat ttagattacg
     1261 ctagggtcaa taaagataaa tttattgaaa caatcatcaa gggaaaaatc cctgatgatg
     1321 aaaaagatgc ggtattcatg gctggaagcc caggagcagg taaaacagag gttgcactag
     1381 gtttagctta aaattatgac aaccatgttg tgattgatgc ggactatttt agagcacaat
     1441 ttccagaata caatggaaag aactcaagtg tttttcagaa agcttcttct tggttagttg
     1501 aacaatcatt aaaatatgta ctggaacatg gctattcttt catactagag gatacttttg
     1561 ctatccttag tgcagagaaa aatattattc gagcccttaa aaataacttt cgcgttacga
     1621 ttttctatgt ttaccaagat cccaaagttg cctgggattt cactcgaaaa cgtgaactag
     1681 ctgaaggacg tcatgtgcca aaagaaatgt ttatcaatgc gttttttaag tccagagaga
     1741 atattgaaaa agtcaaaaat agacatcctg aagtagtcct tcacataatg attaaggatt
     1801 atcaaaacga catttctgaa gtgcattatg cagcagataa tattcaatta gtactaccaa
     1861 tacaatatac ttcaaaagag ttggaggagg aattacatga ctgagaaaga acaagtacaa
     1921 caaatcgtga agaagtataa taaaagtatc gctgatttat cagaaaacgc ctcagcgaaa
     1981 gaatttaaaa cagtaatgaa ttacgtagca gatgaagcaa atcgtaagca gcgaaaacta
     2041 gttgggttgg ataaataaat tttttaaacc atatccttgt aa
//
LOCUS       DAZKKW010000196         2026 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.203, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000196 DAZKKW010000000
VERSION     DAZKKW010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2026
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.203"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..426)
                     /locus_tag="ACG8PO_002889"
     CDS             complement(<1..426)
                     /locus_tag="ACG8PO_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HFQ0208134.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIP"
     gene            complement(535..1566)
                     /locus_tag="ACG8PO_002890"
     CDS             complement(535..1566)
                     /locus_tag="ACG8PO_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0208135.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
BASE COUNT          627 a          330 c          348 g          721 t
ORIGIN      
        1 aggaatctgt tttgattttg ttccatttga aatttctctc actcgctttt ttgtctcatc
       61 atcaaataaa atgaaagggt cttctaaacg atcgataaat acttccattg ttgttgttgg
      121 tagatgataa gaagtcatga caggcactgc accaaggtaa gacacagaaa cagctagaag
      181 gtatgtgtca aaagcggaac tcttataaat aataactttg tctccggatt tcactccaag
      241 atgagccaat tgataagccc gtttcaacac actttcgcag cattttttgt aagttgtttc
      301 aagtcctaat tctgggaaag ctgaaagtgg ctcatcaaag ataatcgggg tctcggaaaa
      361 gttagctgca gcttcttgaa aattcgtaaa taagttcaaa ggggaatacg ttatggtctc
      421 catcattcac actccattat cttttgttaa gtaatataca aattcaagat aatcgtaaca
      481 taatccttta tataataaca tagtttattg tgaaaaaaag atcatacatt tgttttatat
      541 ggaagaaacc cgaacgaatg ccgctaattc gtgataagaa aaatcaacca tcttttccca
      601 gatatcttga aaagaatggg atgagaggta tcgattacca cataaataat ttaccgcgtc
      661 agcatcatct aaatatagtt ctgcataata acgatatgtt tttctgcata gttctggcga
      721 tacgttttta tatactgtaa ttttgctcaa aatttggcga atagtccgag gagaaatttg
      781 ctgatcatgt gaggaacgaa aaacaaaaga attttcgtcc gtattttgtg ctgttaactt
      841 aaaatagttc tccagaaatg gcatcgcttc ttttggtatt ggtactttat tcagtctgtt
      901 ttcttttcta ttcagattaa gaatctgctt ggtcaaagat acatctttac gtttaagttc
      961 tactacttca tggatcttta atccgcaata cagaagaatg gcagtaattg caaggttcct
     1021 cggtctattc ttttcttcaa agctagcttc tctgcttgtt tgaaagcctt caatcgggat
     1081 aaacaaatgc tcaaaatctt tgaaaagttc tgcatcgata tatcgcggag ctgctttggt
     1141 tgtttccggt aaacttgtac ccaaaagaga aatctcttct tcaatcagtc ctttttgata
     1201 caaaaatcgt aaaaaatgat ttaatgaata ttgcttcctt gaaatcccgt aatagctatt
     1261 ttcaaacaat tgctccgttt cttggttcgc tgtagtttgg aacgaacgag tatgtcgata
     1321 gattttagct aagaaattct gataatcttg tttttctaaa tggataatca gctgatcatc
     1381 cacttcttca gaaaatactt ttctcaaata atcagtaaac agatgaaaat cttttgcgta
     1441 ttcatagcga ctgcctaatg aaagggaaga acgattttct tctaaaaact gttgaaagac
     1501 ggaagggagc aagtgtgtac gttgctttaa cttttcttgc agttgttttc tagttttgct
     1561 gtccattaca aattccctcc aatagtgttc attttctata ttttaccgca tttcatcaaa
     1621 tgaagataga cagtttgtct tgatcttagg aagcaaattt ttaactaagt actagctaaa
     1681 agatatgaag taagaagata catttttagt tattttatta gtgcagtcat tcttttgatc
     1741 ttttaattga tttatcctct ttttagctat gtggttactt catgagcaat gaaatggatt
     1801 tggttcgttc taatggcagg aggctctttc ttttatttcc gaaaaattag aaaacatttt
     1861 ttcagaatcg attggctctc catttattca ataatggttt gtctaacgat attgttggga
     1921 taattcttaa agaagtatta gaaatagttg gtacttcatt actttacatg aagatttatt
     1981 ctatggtagg agcaattata ttgagaatta atcctttttt atataa
//
LOCUS       DAZKKW010000197         2002 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.204, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000197 DAZKKW010000000
VERSION     DAZKKW010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2002
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.204"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..377
                     /locus_tag="ACG8PO_002891"
     CDS             <1..377
                     /locus_tag="ACG8PO_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFQ0208136.1"
                     /translation="NVSTEDFFNYCRLEESGVTVTSGTDLPLFITDIPESIIRAVFRR
                     FPEGEEVWEKETSFTTTELLKSFTSNSFKVNGLSEYGEIKVGKSASLAVFSEDLCTDD
                     WQKVMAAEVIATYLDGDLVYKK"
     gene            425..1825
                     /locus_tag="ACG8PO_002892"
     CDS             425..1825
                     /locus_tag="ACG8PO_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HFQ0208137.1"
                     /translation="METMKRKFTFPHSYTLIFILIILAAILTWIIPSGQFETVVENVA
                     GMEKTTVVPGTYHQIDKADYGQGFSSILMAPAEGIISAVEVVAFVLIVGGAFGIILKT
                     GAIDRGLFTLAKVLGDKGILVIPMSMILFSLGGSTFGMSEEVIPLFAVFISLMFSLGY
                     DSMTAILILFLSTQAGYVGSTINPFNVLIAQGVAGVYGNPLLWFRMLSWIVITGISIV
                     FTMLYASRVKKNPLSSIVYESDLKVKHKFQNFDTEKEFSTQDKLIIAGFIVGLGIMIW
                     GIISKGWYMVEISALFLGLGLFAGGIARFSQKEIAEAFVEGCSDFAYAAVIIGLARGV
                     LVILEGGMIIDTILNSLANLLGGLPKFLFTTILLISQVIVSFFVPSSSGAAALTMPVM
                     APLGDLMNVSRDVVVLSNQFGNGLMNIISPTSGVLLAGLAIAEINFSKWFKVGFKIFA
                     ILTLVSAVLLYIATLF"
BASE COUNT          554 a          339 c          432 g          677 t
ORIGIN      
        1 aaaatgtttc cacagaggac ttctttaatt attgtcgatt agaggaaagt ggtgtaacag
       61 tcactagcgg tacagattta ccgctgttta ttactgatat tcctgaatcg attataagag
      121 cagtattccg tcgttttcct gaaggagaag aagtctggga gaaagaaaca agcttcacga
      181 caaccgagct gctgaaaagt tttacaagca acagcttcaa agtaaatggt ttatcggagt
      241 acggagaaat aaaagtaggg aaaagcgcca gcttagctgt tttcagcgag gacctctgta
      301 ctgatgactg gcaaaaagtg atggctgcgg aagttatcgc tacctatctt gatggtgact
      361 tagtttacaa gaaataatgc ttttcgatat aacgttataa aacgaattaa agagaggtta
      421 aaaaatggaa acaatgaaaa gaaaattcac cttcccacat agttatacat tgatttttat
      481 cttaattatt ttggctgcaa ttttaacttg gataattcct agtggtcagt ttgagacagt
      541 tgttgaaaac gtcgcaggta tggaaaaaac aacggttgtt ccgggaacct atcaccaaat
      601 cgataaggca gattatggac aaggtttttc ctctatacta atggcaccag ctgaagggat
      661 tatttcagct gttgaggttg ttgcttttgt tctcatcgtt ggtggtgcct tcgggattat
      721 cctaaaaacg ggagccatcg atcgagggct gtttacctta gcaaaagttc tgggagacaa
      781 ggggatctta gttattccga tgtcgatgat tctattcagc ttaggaggat caacctttgg
      841 catgagcgag gaagtcattc ccttatttgc ggtgtttatt tctctgatgt tttctcttgg
      901 ctatgattcc atgactgcga tcttgattct atttttaagt acacaagcgg gatatgttgg
      961 gtcgacgatt aatcccttca acgttttaat cgcacaggga gtagctggcg tttacggaaa
     1021 tcccctgctt tggtttcgga tgctatcctg gattgtcatt actgggattt caattgtttt
     1081 caccatgctt tatgctagca gagtcaaaaa gaatccactg tcctctattg tttatgagag
     1141 tgatttaaag gttaagcata agtttcaaaa ttttgacact gaaaaagagt tttcaacaca
     1201 agataaacta attatcgccg gttttatcgt aggtttagga atcatgattt ggggtatcat
     1261 ttccaaggga tggtatatgg ttgaaatttc tgcactattc ttaggattag gtctttttgc
     1321 tgggggcatc gctagatttt ctcaaaagga aattgctgaa gcttttgtcg aaggctgttc
     1381 agatttcgcc tatgctgctg taatcattgg gctagctaga ggagtcttgg taatcttgga
     1441 aggtggaatg attattgaca ctattctaaa cagcttggct aaccttttag gtggtcttcc
     1501 aaaattcctg ttcactacga ttctactcat ttctcaagta attgtttcct tttttgttcc
     1561 atcctcctca ggagctgcgg cattaacgat gccggttatg gcaccattgg gtgatctaat
     1621 gaatgtaagt cgtgatgttg ttgttctaag taaccagttt ggaaatggtt taatgaatat
     1681 tatctcccca actagtggtg tattactggc aggattagcc atcgcagaga ttaatttctc
     1741 taaatggttt aaggttggct tcaaaatatt tgccatctta acccttgttt ctgctgtttt
     1801 gttatacatt gctacactct tttagtctta atcatccatt aataggaggc aatagttatg
     1861 attaaaataa atcaattagc aggaatgaat atccactatt tattttattc tctcaaagaa
     1921 tttttcgctt atcaacagaa aattggtatt tcttctgttg aactttgggg aggtgctccg
     1981 cattttttca tagatgcttt ta
//
LOCUS       DAZKKW010000198         1955 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.205, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000198 DAZKKW010000000
VERSION     DAZKKW010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1955
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.205"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..565
                     /locus_tag="ACG8PO_002893"
     CDS             <1..565
                     /locus_tag="ACG8PO_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="C47 family peptidase"
                     /protein_id="HFQ0208138.1"
                     /translation="FSKKQNFPAVKRVKSVSSGDNFIMINWRIAETQTSFPWCAAYVE
                     AAILCNKTDVRATSARAIMHYTYPNLNNSQIESKSLNQDQIIKYANYCGVYPKKIARI
                     LNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHALALFGWVNQGGYQTYYVWNPWYNYPT
                     VISANAGPVTIPVPGGGFTWDNTITNW"
     gene            538..981
                     /locus_tag="ACG8PO_002894"
     CDS             538..981
                     /locus_tag="ACG8PO_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0208139.1"
                     /translation="MGQYHYKLVNKLIALVMGLVLVLFLTSCSKKQEIQKTDLIPFLG
                     TWQTKDHSERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNL
                     LTLRSNKKQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
BASE COUNT          652 a          339 c          371 g          593 t
ORIGIN      
        1 tttttctaaa aaacaaaatt ttcctgcggt taaacgtgta aaaagtgtct cctccggaga
       61 taactttatt atgattaatt ggcgtatagc agaaacccaa acaagttttc cgtggtgcgc
      121 tgcttacgta gaagctgcta tcttatgtaa caaaacagat gtacgtgcta cttcagctag
      181 agccattatg cattatacct atcctaattt aaacaacagt caaattgaat caaaaagtct
      241 taatcaagat caaataataa aatatgcaaa ttactgtggt gtttatccta aaaaaatagc
      301 acgaatacta aacctttcag aggtccaaac acaactaaga aaaggaaatg ccatttatat
      361 gggaactgct ggttatggaa attatagtaa cagccgtcat gcacttgcat tatttgggtg
      421 ggtcaatcaa ggtggttatc aaacttacta tgtgtggaat ccttggtata actacccaac
      481 tgtaataagt gcaaatgcag gaccagttac aattcctgta cctggaggtg gttttacttg
      541 ggacaatacc attacaaatt ggtaaataaa ctcattgctt tagttatggg tctagtgtta
      601 gttcttttct taactagttg ttcaaaaaaa caagaaatac aaaagactga tctgattcct
      661 tttctaggaa cttggcaaac taaagatcat tcagaacgtc tagtaatcac taatttaaat
      721 acttcaaaaa atatagaaca atataccatc ttagtaaaag agaacaatca taaaattcaa
      781 atcattgata aaaatttaaa tataaatgaa caagttttca tttacaaagc aacaaataat
      841 ttactaacac tgcgctcgaa taaaaaacaa ttagattaca cttttagttt ggattctcca
      901 aaaaagctcg tatttatttt gacaacgaat gatgggacag aagggacagc taaaccaatt
      961 gaatttttta aagctgattg atgaatagtt gtttgatatt acaaaacaat aatttcaccg
     1021 aaggtgaaac aagcgaaact ttacaaaaag tggctagcat ttgctagcct tttttgtatt
     1081 gtggagcgtg ggttttgagg gggattcaag ggggagcttg cgcccctttg acaagcggtt
     1141 atttgcacca aatacgtagg tatttggcta gcaaatatag agcttgccaa gccctttagt
     1201 gtgttttgta gaaagggacc ttatagtcac tttcacatta caaacgatac gtcctatgga
     1261 cgtatcaaaa gggtgggact tatgagcaaa ttaaagcaaa atttggctag caatccttct
     1321 aaaaaataca cctattgatc agagtcaaaa taaatcagtt ttcgagtgag cgaatccaaa
     1381 tttgcaactc tgaagctttg caaatgagtg tgccggcttc cgtaaaagtc taggtaacat
     1441 tagtcgatat atacataggg ctctaatttg cgttttaagg cgttttaaac gttctggtat
     1501 gtaattatac caaagcgact ttaaaacgcc ttaaaacgaa ttttagaggt tctgtagaac
     1561 gttttagggt tgttagggct tgccctgacc gcctgtaagg cgctttaaaa acaagataag
     1621 ggattttatc tagacttgtc ttaaaacgcc gtagatgagc gctcagagct tctaaaagga
     1681 ctccttacga actgttttta agcaaaggag cctttaggcg actgcgcagt ttaatgtgag
     1741 agcgtagcga tcacaccttt aaagcgaccg tagggagcag attcccagtt taggaaattt
     1801 cgcgaacctg tttggagaaa aaaagtagga aaattttagg cgggaaattt tgcccccctc
     1861 ttttataatc tcttttataa cctcttttta aacctcttta ccgtcggctg tatccttact
     1921 ctcccaaggt gtgcagaatc ttttaggggt ctttt
//
LOCUS       DAZKKW010000199         1946 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.206, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000199 DAZKKW010000000
VERSION     DAZKKW010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1946
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.206"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..154
                     /locus_tag="ACG8PO_002895"
     CDS             <1..154
                     /locus_tag="ACG8PO_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFQ0208140.1"
                     /translation="EENIVKEMPESANDMYEKELKYFFENVVTGKENWNDLYHANEVL
                     KIAEEK"
     gene            155..907
                     /locus_tag="ACG8PO_002896"
     CDS             155..907
                     /locus_tag="ACG8PO_002896"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HFQ0208141.1"
                     /translation="MEILFTICGRAGSKGFKNKNLKYLAGKPLVHFALAMIDIYQQKH
                     PEHNCTTCLNTDSNELVELTTKLNPSVKIVERKPELATDTVGKIEVIRDSFLQLERED
                     NIFDLIIDLDLTSPLREVSNLEELIDEHLKNLDKDVYFSVVSARRNPYFNMVKRIENE
                     VTIVNKSRYTSRQQTPEVFEMNASMYSFKPEFLKKSNYIFDGQCGIIKMRDYLILDID
                     SEEDFKWLEFLFPKFLEDSSELQDIYNYLYRR"
     gene            912..1859
                     /locus_tag="ACG8PO_002897"
     CDS             912..1859
                     /locus_tag="ACG8PO_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFQ0208142.1"
                     /translation="MSCYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMT
                     KNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMI
                     LELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYC
                     HLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSR
                     DFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEE
                     RSGDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
BASE COUNT          679 a          263 c          389 g          615 t
ORIGIN      
        1 tgaagaaaac attgttaaag aaatgcctga atctgctaat gatatgtatg agaaggagtt
       61 gaaatatttc tttgaaaacg tagtaacagg caaagaaaac tggaatgatt tatatcatgc
      121 aaacgaagta ttgaaaatag ctgaggagaa ataaatggaa attttattta ctatttgtgg
      181 tagagctggg tctaaggggt ttaaaaataa aaatttgaaa tatttggcag gaaagcccct
      241 agtccatttc gctttagcaa tgatagatat ctatcaacaa aaacatcctg aacataattg
      301 cacgacttgt ttgaatacgg acagtaatga gttagtagaa ttgacgacta agttaaatcc
      361 gtctgtgaaa attgtagaac gtaaacctga attagctaca gatactgtag ggaaaattga
      421 agtgatacgt gattcattct tacaactaga aagagaagat aatatatttg atttaattat
      481 agatttagat ttgacttcac ctttacgtga agtttctaat cttgaagaat taatagatga
      541 acatctaaaa aatttagata aagacgtcta cttttcagtt gtttctgcaa gacgtaatcc
      601 ctattttaat atggtaaaaa gaattgaaaa tgaagttact atagttaata aatcaagata
      661 cacatctagg caacagacac ctgaagtttt tgaaatgaat gcctcaatgt attcttttaa
      721 acctgaattt ctaaaaaaaa gtaattatat ttttgatggg caatgtggga taataaaaat
      781 gagagattat ctaattttag atattgatag tgaagaagat tttaaatggt tagaattttt
      841 gtttcctaaa tttttagaag attcttcgga gcttcaagat atctataatt atttatatcg
      901 gaggtaaaag gatgagctgt tatcttatca ccggtggggc tggcttcatt ggttctacct
      961 tagccaatca tttaggagaa gaacatgtgg taatcgtcgt cgatgactta tcaatgggaa
     1021 aagtagaaaa tcttgatatg acgaaaaata tcacttttat tgagggagat gtcgctgaga
     1081 aatccttgat ggaagagatc atgaaagcta ataaatttga ttatatcttt catttagcag
     1141 cagttgctag tgtggcagat tcagttgccc gaccagtaga aacacatcgt gtgaattttg
     1201 aaagtgtatt aatgatttta gaattgatcc gcaagtatca acctgattta aaacggatcg
     1261 tgtttagttc ttcagcggct gtttatgggg atgaaccgac tttaccaaaa aaagaagagt
     1321 ccgtgattcg cccattgacg ccttatgcgg ttgataaatt cgctgcagaa caatatgtgc
     1381 tagattattg tcatttatat gatgtacccg gtagcgcagt tcgttttttc aatgtctatg
     1441 gtccaaatca aaatccgaat tctccataca gtggtgtgat ttctattctc gtagatcgct
     1501 ataagaaaca aatcgtagga gaagcaactt catttacatt atttggtgat ggaagccagt
     1561 ctagagactt tgtttatata gatgatgtaa tccaagcatt acttttagtt gcgaatgagg
     1621 aagctgcact ggggaaacaa ttcaatgttg gaacgggtaa agcaaccaca cttttggaat
     1681 tgattcatgc aatcaatgaa attctcggtg tagaattgac acttgaatac aaagaagagc
     1741 gttcgggtga tatccatgac tctctagcag atattactaa gatccagtca attggctatc
     1801 aaccgaaata tggtatttta agtggaatga ctaattattt gaaaactgaa atcaattaaa
     1861 acatactaag ataagattag agggtaaatt caggctcttt gtcaataagg actgatgagt
     1921 tttgtaaaat caaatctggg atagaa
//
LOCUS       DAZKKW010000200         1930 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.207, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000200 DAZKKW010000000
VERSION     DAZKKW010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1930
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.207"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..435
                     /locus_tag="ACG8PO_002898"
     CDS             <1..435
                     /locus_tag="ACG8PO_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HFQ0208143.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPVQAEQPVVQET
                     VQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQL
                     DSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
     gene            complement(759..842)
                     /locus_tag="ACG8PO_002899"
     tRNA            complement(759..842)
                     /locus_tag="ACG8PO_002899"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(806..808),aa:Leu,seq:cag)
     gene            1370..1870
                     /locus_tag="ACG8PO_002900"
     CDS             1370..1870
                     /locus_tag="ACG8PO_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0208144.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
BASE COUNT          625 a          364 c          329 g          612 t
ORIGIN      
        1 tactcaggac aacaattaac aatcccaaca gaaggctctg cacaagcagc tgctgaacct
       61 caagcagccg ttcaagaagc accagtccaa gctgaacctg ttcaagctga acaacctgtt
      121 gtacaagaaa cggttcaaac ggaaacacaa gcagcgcctg ttgctgaaac acaacctgct
      181 cctgctgtaa cagaaactgc tgctactcca gcaagtacaa gctctgcaaa agaatggatc
      241 gcacaaaaag aatcaagcgg ttcttacaca gcaacgaacg gacgttacat cggccgttac
      301 caattagatt cttcttactt aaatggtgac tactctgctg caaaccaaga aagagtagca
      361 gaacaatatg ttacttcacg ttatggttct tgggaagcgg cgaaagcatt ctgggaagca
      421 aacggttggt actaagattc attatttgaa cgtttaaaca cattcaaaat atagtaagag
      481 gtcatgcgat ttattctttc gcatgacctc tttttgagtt taagtttagt ctttattcct
      541 taactatcgt ttgccagtat ttcttgtatc agaatctata aatctactat ttgaatcaat
      601 ctattaggtt ttaaactgtc ccaccttcac acaaattttt tttgatttat ttgaatctgg
      661 ttccgttgtt taaaattttt cctatattct tttagataat atgttatttt ctttctcatc
      721 gctccaggtg taccttctct ttataaaaaa acaaattatg cggctaagaa gactcgaact
      781 tccacgaggt caccctcact agcgcctgaa gctagcgcgt ctgccaattc cgccatagcc
      841 gcatcacatc ttttagttta ggattacatg tgttttttgt caagaacata tttttgttct
      901 ttttcatttc tgtacgccct cccttatgcc atctacacaa ataattcatc cgagcaactc
      961 catcatctat tgagggttgg tgaaaaaatc tgttttatcc atatgatcga tttcaatgtt
     1021 aaacgaacga ctctgggatt agtaactatt ttattttttt cttcatttct tttttcctct
     1081 ttttctcgat tcagctatct tctttcattt ggatattgat tatttcttct gttgattact
     1141 atccttctgg aattctagac gctgaagtaa ttcacgtttt ctttcttcta gagatcataa
     1201 gtaacgaatg aacgaacttc aaataatttt tttattgtaa ttcatatttg tattgttcga
     1261 tgattcttaa tacgatatga cacaataaat aaaaataggc atggctattt acaacaatag
     1321 tgctatagta gaaaacaaat gaataaggtt acttaaagag gtgaatacga tgaaactgat
     1381 caaactatgg gaagcagatt taaagaaagc ttatcagcta caaactagct ttggaccaaa
     1441 tgagagtgga tttatcaaca atgcttatgg ctatacattt gctgaatttt tagattatgt
     1501 aaaaaaatgc gaaaaaacgt ctttaggaat cgatcttcct gagagttacg ttcccgcaac
     1561 cgtctttatt ttagaaagtg atgaacatga gtatgtagga atttttaatt tgcgccataa
     1621 actgaacgaa acattaagaa acggcgctgg ccatattggt tacggcatct cccctaatta
     1681 tcgtaaaaaa ggatatgcca cacaaggatt agcattagta ctacaagagg caaaaaaaat
     1741 cggcattgat gaggcacttt tgtctgtaaa taaaaaaaat cttggcagtc ttaaagcaca
     1801 acaaaaaaat ggggctgcga tttatcgtga agatgagaaa gaatactata cgaaaatcaa
     1861 actgatgtaa aaaagaagga ttccaaaagg aaagggattt gaatgtcctt tatcaataga
     1921 acacatttat
//
LOCUS       DAZKKW010000201         1913 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.209, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000201 DAZKKW010000000
VERSION     DAZKKW010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1913
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.209"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(29..1462)
                     /locus_tag="ACG8PO_002901"
     CDS             complement(29..1462)
                     /locus_tag="ACG8PO_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008754 - O antigen ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HFQ0208145.1"
                     /translation="MNRIVPVLEKSLLSRDAFRVINLIICLLYFMPSTFNVTNIPMKI
                     AFAWGGIIVLYEVFIKKNFFKMRFSWLLIGAMISFILTIIINRENFLIPSIYNFGYLL
                     ITLIVIFPADFSQSEEEKKKKMVYFNNVFIIIIFLAALISIYQFIFLISYHVPTGTPG
                     LLARQGFIEHRLFGVYTSPNVGAMFGYTSIILSLISVLISKITKTIRVFYMMNFIVQI
                     FYITLSSSRGAQVVICSFLVLFLLLFANSKFRKSIREVLPVKIKWMVSILLVLLVYFS
                     FGTNYAKDFLATIPVSIDRKVEVIESNESDKTPLPEKEVILQHSSDNSEISAGRFTIW
                     KAALKVIRPTLLFGYGETDFYGNSDAKFLKKVDLDTTDVNELKRAHGNMHNGFLQVLV
                     SSGIIAFICIYAFYVLNIFSLLKTFFRSKVNYAFLGIVLIFILSIFIDEMVEAHVLFN
                     KRDVISIVFWYYLGSISYLYLKKGELI"
     gene            complement(1455..>1913)
                     /locus_tag="ACG8PO_002902"
     CDS             complement(1455..>1913)
                     /locus_tag="ACG8PO_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-1,6-N-acetylglucosaminyltransferase"
                     /protein_id="HFQ0208146.1"
                     /translation="MIKLPPKIRHHKFDLYHGSQWWALSKECLTELIQMYEQNQQDYL
                     NFKIGMFAPDEKFFHTLFFNSSFKNKNVIGGPDLPLELKNIEETTLQTSKLANIHIID
                     PSMNKWFDERDIDQIKESEKLFVRKVKSDYSDKLLNRIDKEILKVGNIDE"
BASE COUNT          723 a          312 c          229 g          649 t
ORIGIN      
        1 aaaggagtcc aagcaatgaa agctgtcttc atattaattc tccttttttt aaatacaagt
       61 aagaaatgct tcccaaataa taccagaaaa caattgaaat gacatcgcgc ttattgaaaa
      121 gtacatgagc ctccaccatt tcatctataa aaatagatag tataaaaatt aaaacaatac
      181 caagaaacgc ataattcact ttacttctaa aaaatgtttt gagtaaacta aagatattta
      241 acacatagaa agcatagata catataaaag caattatacc acttgataca agtacttgaa
      301 gaaatccatt atgcatgttt ccatgtgctc tttttagttc attcacatct gtagtatcaa
      361 gatctacttt tttcaaaaat tttgcatcgc tattcccata gaaatcggtc tctccataac
      421 caaataacaa agttggtctt ataactttaa gcgcagcttt ccaaatcgta aaacgaccag
      481 ctgaaatttc ggagttatca ctagaatgct gtaatattac ttctttttct ggaagtggag
      541 ttttatctga ttcatttgac tctataactt ctacttttct atcaatcgat acaggaattg
      601 ttgctaaaaa atccttagca taattagtac caaaagaaaa atataccaat agaactaata
      661 atatgcttac catccatttg atttttacag gtagtacttc tctaatgctc tttctaaatt
      721 ttgaattggc aaacaacaaa agaaataata ctaaaaaaga acaaattact acctgggcac
      781 ctctagacga agaaagtgta atataaaata tttgtacgat aaaattcatc atataaaaaa
      841 cacgaatcgt ttttgttatt tttgaaatta atacactaat taaacttaaa ataatagaag
      901 tatatccaaa catagctcca acatttggac ttgtatagac accaaataac cgatgttcaa
      961 taaaaccttg tcttgcaagt aatcctggcg ttcctgttgg gacatgataa gaaatcaaaa
     1021 aaataaattg atagatagat attagcgcag ctaaaaaaat tatgataatg aaaacattat
     1081 taaaatacac catttttttc tttttttcct cttcactttg tgaaaagtct gcaggaaaaa
     1141 ttactatcaa agtaatcagc aaatatccaa aattataaat agatggaatt aaaaaatttt
     1201 ctctgttaat gattatagtt aagataaaac taatcattgc tccaatcagt agccaagaaa
     1261 aacgcatttt aaaaaaattt ttctttataa aaacctcata caaaactata attccacccc
     1321 aagcaaaagc tattttcatc gggatattag ttacattaaa agtactaggc ataaaataaa
     1381 gcagacaaat gattaaattt attactcgaa acgcatctcg acttaataaa ctcttctcta
     1441 aaacaggtac tattctattc atctatattt ccaactttca aaatttcttt atcaattcta
     1501 tttaatagtt tatcagagta atccgattta acttttctta caaataattt ttcactttct
     1561 tttatttgat ctatatccct ttcatcaaac catttgttca tggaaggatc aataatatga
     1621 atatttgcta atttcgaagt ttgtaaagta gtttcttcaa tatttttcaa ttcaagtggt
     1681 aaatctggtc ctccaataac atttttattt ttgaacgagg aattaaaaaa aagtgtatga
     1741 aaaaattttt catctggggc aaacatacct attttaaaat tcaggtagtc ttgttgattt
     1801 tgttcataca tttgtattaa ttctgttagg cattctttcg acaaagccca ccattgagaa
     1861 ccatgataca aatcaaattt atggtgtcta atttttgggg gaagtttaat cat
//
LOCUS       DAZKKW010000202         1906 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.210, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000202 DAZKKW010000000
VERSION     DAZKKW010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1906
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.210"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            466..1425
                     /locus_tag="ACG8PO_002903"
     CDS             466..1425
                     /locus_tag="ACG8PO_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFQ0208147.1"
                     /translation="MTYTHLTTDELVIIESYFKMNQSVAKTAHCLNRSRQTIHKVYLF
                     FKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIS
                     CSARTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQFSNEF
                     GHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIEKKLNQWFESVPKNLFK
                     SITFDCGKEFSNWKQISNVNDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNS
                     VDEFFIQSVASKRNNIPRKSLDYRTPLEVFLSYVSIDDLSNLI"
     gene            1519..1743
                     /locus_tag="ACG8PO_002904"
     CDS             1519..1743
                     /locus_tag="ACG8PO_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
                     /protein_id="HFQ0208148.1"
                     /translation="METEWQRRERKKQKKEEMLRLCRLLDVKDWDKNEQIAQKVRSII
                     HKEEKVKNKDTRRTENEKRKVQKKREKSKS"
BASE COUNT          722 a          291 c          341 g          552 t
ORIGIN      
        1 tatttgtaat ctatagtatc agtcctataa aataaacatc taattaatta ctctaaatcc
       61 taattataag ccttttttct cacttttttt caaaaatatg gtatgcttct tagtaaattc
      121 aaatatatgg tatgcttctt agtgaattca aatataaaag agtttaaagc gtaatacact
      181 tatggggaag tggtttgggg tgcgctttaa actcttcttt attattatct cacagcatgc
      241 ttaaaaggtc tatggattaa gttatgtaaa aaagtgcctc tttaaacgtc ctaaaatcca
      301 aacaaggata gcataatgta cactatcgtt gatccagaaa acaggtaaat aaattttgca
      361 aaagcaataa gtcgccgatt gtaaaattaa gctagacaac taaaaaagtc atttgtgcta
      421 cactcaaaat agttccgacc aaagaattat aaggagtgag tacaaatgac ctatacccat
      481 cttacaacgg atgaacttgt aataatagag tcttatttca aaatgaatca atctgttgct
      541 aaaactgccc attgcttgaa tcgttcaaga caaacgatcc ataaagtata tctgttcttc
      601 aagcaaggaa aatcagcctt agaatattat caacagtata agaaaaacaa atcaaactgt
      661 ggtagacgtc cgcttgtttt acccgaggaa caatcagaat atattcaaag aaaggttgtt
      721 caaggatgga cacccgatgt gattgttggt cgtgcagcgt ttcctattag ttgttctgct
      781 cgtaccattt atcgtatgtt caaaaagggg ctatttgatt cttctgactt accgatgaaa
      841 ggcaagcgta aaccgaatgg acatcaagaa agacgtggaa aacaaacttt ccgccgctct
      901 attcatgaac gtgaaaagga ttatagccaa ttctcaaatg agtttggtca ccttgaaggt
      961 gacactatcg taggtctgaa acataaaagt gctgtaatta ccttagttga acgattatca
     1021 aaagttatca tcacattgaa accgtgtggt agacaagcga ttgatattga aaaaaaatta
     1081 aatcaatggt ttgaatctgt accgaaaaac ctattcaaat ccatcacttt tgattgtgga
     1141 aaggaatttt caaattggaa acagatcagt aatgtcaatg atattgccat ttatttcgct
     1201 gatccaggaa cgccgtctca aagaggccta aacgagaatt ctaacggatt gttacgtaga
     1261 gatggtttat tgaaatctat ggatttcaat tcagtagatg aattttttat tcaatctgtc
     1321 gcatctaaac gaaataatat tcctagaaaa tcactggatt atcgaacacc tttggaagta
     1381 tttttgagtt acgtaagtat tgatgatctg tctaacttaa tttgacaatt aagaatatta
     1441 aaatcataga aatgtctaat caaaaaaata taaaaattat tttgcgtaca tatgattaaa
     1501 gaaaggagag tgtgacacat ggaaacagaa tggcaaagac gtgaaagaaa aaaacagaaa
     1561 aaagaagaga tgttacgatt atgtcgatta ttagatgtaa aagactggga caaaaacgag
     1621 caaattgcac aaaaagtacg atcaatcatc cataaagagg aaaaagttaa aaataaagac
     1681 acgcgtagaa ctgaaaatga aaagagaaaa gtacaaaaga aaagagaaaa atcaaaaagc
     1741 tagaaagaaa aagaggctga gacagaagcg tttaactcca agaaataagc tggaattcac
     1801 aaaaatttga aaagcaattt tcgtgaattt cagcttattt ccgaaggagt tgcttctgct
     1861 ttcgccgttt atacgtgttt agagcgtgag acaaaagtgt tcttta
//
LOCUS       DAZKKW010000203         1869 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.211, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000203 DAZKKW010000000
VERSION     DAZKKW010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1869
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.211"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            306..560
                     /locus_tag="ACG8PO_002905"
     CDS             306..560
                     /locus_tag="ACG8PO_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFQ0208149.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEGNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            561..878
                     /locus_tag="ACG8PO_002906"
     CDS             561..878
                     /locus_tag="ACG8PO_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFQ0208150.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
     gene            1437..>1869
                     /locus_tag="ACG8PO_002907"
     CDS             1437..>1869
                     /locus_tag="ACG8PO_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0208151.1"
                     /translation="MNILNEKRYVWFAGKKYITTFKRLSELMQQTVKSAMPEGYKFNF
                     VQSSVSTESIYFSILSESRLFHFRISSHVKKTDVDIYTFRTNNYKNLNDLSRSISEYL
                     KGNSYLKIRFSHILILLPYLNENFTLIQVDKSFIASGNQGNM"
BASE COUNT          655 a          280 c          350 g          584 t
ORIGIN      
        1 aaggcaatac gccttagttg tcggtcttcg agttaccaac ggtaaccctc ataccgaacg
       61 gcattcatca cccacctata gaggatgggc gacttctgcc taattctgtt aaatggatgt
      121 taacggaagt gtatggtgag tagttcactc gtattttgat atcgtttgta tatgttttca
      181 gcgttctaag tggcttattt tgtccaataa taatatggag tggaatccct taagaaacac
      241 cgttggcaat tgttgttata acatgttata atttattcat caaaaactaa aaggagtgtg
      301 ataacatggg aaaagaacta aaggttagaa agatcggcaa tagcgtggga gtgatccttc
      361 caagctctct tggattaaaa agcggagaca ttatccaagc caaacaagaa ggcaacttat
      421 tcatattaga tactactcaa atagcaaaag aacacgatcg caaattgatt gaagaaagtt
      481 tccaagactt tgagaaaggt ttaacagttt cagaaattga aatggtaaaa gctttcggaa
      541 aatacggctg gagtgaatag atgaaagagt acacgctagt ttattctaaa agttttcaga
      601 ccagccttga aacgataata gaagaatggg aaaatgagct tgctctttct ccagccagca
      661 taaaaagatt tgtaggtgcg atccagaagg ctttagaact aaccaaaaat tttccaacta
      721 tgcatgagga agtttccgaa atctatggaa tggatatccc aacatatcgg atcgtgatcg
      781 gcaaaagcta tgccttattt tatcggatcg accatgaaaa aaaagagata cttgtaggac
      841 gtattttcca atctaaacaa atgaaactag gcttttaaca ataattagga aaaagtcgct
      901 catcctctac aggtgggtga ttaatgccgc tcgatatggt tgttaaccgt cggttactcg
      961 aagattgata tataagactt agtacctttt tattttgctc catgttatgg ccgaaggtct
     1021 aaagatacaa tcattagtct agagaaaaat taagaaccgt aggaactacg gggatcgctt
     1081 ggtgaataag agacactgct attagtgaaa aaataagata tcaagtacgc tctattccca
     1141 aaaatttcgt gactttactc atgagaggtt caagtagtat atttttaatc aaaattgacg
     1201 taacttttaa taaatactac ctaaataagg tagtatttat ttttgtgttt taaaagagga
     1261 agtccaatat atctatggga aacttcttaa atattatgtt ggtacatgaa aatatacatc
     1321 cctgctagac tatttacaac attttacttt gtacgtattc ttgttttaaa aggaatggga
     1381 taaaatatag gtacaataac tatttgatgc taaatataat taatggagga gagatagtga
     1441 acatacttaa tgaaaaaaga tatgtatggt ttgctggtaa aaaatatatt actactttca
     1501 aaagattatc cgagttaatg cagcagactg ttaaaagtgc tatgcctgaa ggatacaagt
     1561 ttaattttgt tcaatcttca gtatctactg aaagtatcta tttttcaatt ctttctgaga
     1621 gtaggctttt tcattttaga ataagttctc atgtaaagaa aactgatgtt gatatatata
     1681 cgttccgtac aaataattat aaaaatttaa atgatttgag tagaagtatc agtgaatatc
     1741 tcaaggggaa tagctatctc aagattcgat tttcacatat attaatttta ctaccatatt
     1801 tgaatgagaa tttcacccta attcaagtag ataagtcttt cattgcctca ggtaatcaag
     1861 gaaatatga
//
LOCUS       DAZKKW010000204         1835 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.212, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000204 DAZKKW010000000
VERSION     DAZKKW010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1835
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.212"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1443)
                     /locus_tag="ACG8PO_002908"
     CDS             complement(<1..1443)
                     /locus_tag="ACG8PO_002908"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HFQ0208152.1"
                     /translation="MTEQIEKNSKSLYQALWNSADILRSKMDANEYKSYLLGLVFYKY
                     LSDNMLRYVSVLLEEETDDLNKAQDLYVEAFKDADIKDDLLYELKDEFSYTIAPALTF
                     TAQVAAIHDGSFQLEDLVQGFRDIEQSSENFENLFEDIDLYSKKLGSTPQKQNKTIAD
                     VMKELEGLNMAGHAGDVLGDAYEYLIGQFASESGKKAGEFYTPQPVAKLMTQIVLQGK
                     EDKKGFSVYDATMGSGSLLLNAKKYSHQPGTISYFGQELNTSTYNLARMNMILHGVPI
                     ANQHLHNADTLDQDWPTEEPTNFDGVLMNPPYSAKWSADKGFLDDPRFSAYGVLAPKS
                     KADFAFLLHGYYHLKDTGVMAIVLPHGVLFRGGAEGKIRKALLENGAIDTVIGLPANI
                     FFNTSIPTTVIILKKDRAKKDVLFIDASQDFEKIKTQNTLRDYHIDAILEAYKTRTIK
                     DKYAYVAEFDEIVENDYNLNIPRYVDTFEEE"
BASE COUNT          521 a          346 c          330 g          638 t
ORIGIN      
        1 ttcttcttca aaggtatcca cgtaacgggg aatgtttaag ttgtaatcat tctccacaat
       61 ttcatcaaat tctgcgacat aagcatactt gtctttaatt gtacgggttt tataggcctc
      121 taaaatagcg tcaatatggt aatctcgtag cgtattttgc gtttttattt tttcaaaatc
      181 ttgtgacgca tcaataaaga gcacatcttt tttcgcacgg tctttcttca agataattac
      241 ggtcgttgga atggacgtat taaagaagat gttagcaggt aagccaatga cggtgtcaat
      301 ggcaccattt tctaacaagg ctttgcgtat cttcccttct gcgccccctc taaagagcac
      361 cccatgtggc aaaacaatgg ccatgacgcc cgtatctttc aaatgatagt agccatgtaa
      421 taagaaagca aagtccgctt ttgatttagg ggctaatacg ccataggcag agaagcgtgg
      481 gtcatctaag aagcctttat cagccgacca ttttgcagaa tacggtgggt tcatgagcac
      541 cccatcgaag ttcgttggct cttctgttgg ccagtcttga tccaaggtat ccgcattatg
      601 caagtgttgg ttggcaatgg gaaccccatg caaaatcatg ttcatacgcg ccaaattata
      661 ggtagacgta ttcaactctt gtccgaaata agaaatggtt cccggttgat gggaatattt
      721 tttagcgttc aaaagtaacg agcccgaacc catggtcgcg tcgtaaacag aaaagccttt
      781 tttatcttct tttccttgta aaacaatttg tgtcatcaat tttgcgaccg gttgtggggt
      841 atagaattca ccggcttttt taccggattc agatgcgaat tggccaatta agtactcata
      901 ggcatcgcct aagacatcgc cagcatgccc cgccatgttt aacccttcta attctttcat
      961 aacatctgcg atggttttat tttgtttttg tggggttgac cccagttttt tagaatacag
     1021 atcaatgtct tcaaataagt tctcaaagtt ttcgctggat tgttcaatgt cacggaaacc
     1081 ttgtaccagg tcttctaatt gaaaagatcc atcatgaatg gctgccactt gcgcggtaaa
     1141 ggtcaatgcg ggtgcgatgg tatatgaaaa ctcatctttt agttcataca agagatcatc
     1201 ctttatatct gcatctttaa atgcttcaac gtaaaggtct tgtgctttat ttaggtcgtc
     1261 cgtttcttcc tccaatagaa cggagacata ccgcagcatg ttgtcggata agtatttata
     1321 aaagaccaag cctagtaaat agctcttgta ttcattagcg tccatttttg aacgcaggat
     1381 atctgcggaa ttccacagtg cctgatataa gctttttgaa tttttttcga tttgttctgt
     1441 cattggttat tttcctcatt cgtgttgtat tttttataaa tcagtttttt aaaaaattag
     1501 ttactatttg aacttgtttt ttcaccatat gtattttaat gaaatggata agctacattt
     1561 gcttagacaa ttcatgtctt acttaattct tattataaca aacctatttc acctttttcg
     1621 atacattaaa taataagtaa cttaaaataa ggttcgaata accttgttaa atagtggttt
     1681 tcttatctta tagtatagaa atcaagagat tacttccgat ataacatttt tttaaaaaaa
     1741 ttagattgta atgtttttta tattcattct aaattttcac ttaaaaagga taatcattaa
     1801 aatcatcaaa ttcagtaata tatattttct tactc
//
LOCUS       DAZKKW010000205         1826 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.213, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000205 DAZKKW010000000
VERSION     DAZKKW010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1826
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.213"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..199)
                     /locus_tag="ACG8PO_002909"
     CDS             complement(<1..199)
                     /locus_tag="ACG8PO_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0208153.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQ"
     gene            complement(199..405)
                     /locus_tag="ACG8PO_002910"
     CDS             complement(199..405)
                     /locus_tag="ACG8PO_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HFQ0208154.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYRLYQC
                     PTCGKKYTLSYGDYVLIDEKVKKR"
     gene            complement(842..1102)
                     /locus_tag="ACG8PO_002911"
     CDS             complement(842..1102)
                     /locus_tag="ACG8PO_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0208155.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIESMQIRNPDHFTRSMGI"
     gene            1276..1380
                     /locus_tag="ACG8PO_002912"
     CDS             1276..1380
                     /locus_tag="ACG8PO_002912"
                     /inference="COORDINATES: protein motif:HMM:NF028245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HFQ0208156.1"
                     /translation="MPVETALGLMISFGSLIATIIFGILAVVNNNQKK"
     gene            complement(1521..>1826)
                     /gene="ssb"
                     /locus_tag="ACG8PO_002913"
     CDS             complement(1521..>1826)
                     /gene="ssb"
                     /locus_tag="ACG8PO_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFQ0208157.1"
                     /translation="MATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQLLE
                     SKAATESRAHADQSSSSPSPTTFEQRDVATPNNNGLNASQNPFGGQSIDISDDDLPF"
BASE COUNT          503 a          358 c          352 g          613 t
ORIGIN      
        1 cttgataatg cagttcatat cctgcaatct tcactaggtc ttcaaatagc atagtcggtg
       61 catcctctac taaaggggtt tctatcgtcc agtaccgcgg aataagtaaa gcttctgcta
      121 ctttttcaga aatgacttct ttgtccacca aaatttgaac gacttctttt acagtcaata
      181 atttgtgttg tgttttcatc accgtttctt tactttctca tcaatcaaaa cataatcgcc
      241 ataagacaag gtatattttt tcccgcatgt tggacattga tacaaacgat agcttttgat
      301 acatttcgct ctttctccac aatcgcattc gacagtatgt ggtgcatgat agtttgggaa
      361 taattcgctg aagggaacac cccaataatc aaatttcttt cgcatgattg attctgcctc
      421 ctcttaggaa aggttttatt tcctttcgtt agttctttct ttattgattt gttggccaga
      481 atgaatcaac gaaaagcgac aaggacaagg gcgcgcagcg tgccatttac ccttgtcctt
      541 gtcgcttttt ggtgataaaa ggcaacttgt tgccggtctg gcaaatcaat aaagagatag
      601 cgaacaggaa cgaacctttt ggtgagagga agttttttct attttgtctt gctttgcaag
      661 acaccatatc ttagtgaacg tgcaacgttc acttcctttc ttctcgaacg gcggtggcgg
      721 ttggagtgag agaaaggttt ttcaattgag aaggcacttg tgtcttccta aaaaggtctt
      781 ttcctgtttc tatgtaaaac aaaaagagct gcaatcctca tttaggactg cagctcttct
      841 tttatatgcc catcgatcgc gtaaaatgat cggggtttct aatttgcatc gattcaatga
      901 ctaattgatt ccgtttattg aaatgacctg tgacggcaat attgtatttt ccatcatcca
      961 taattaacaa tttatcgata atttcaatat ctgcaactac acaattaatc gattcatgaa
     1021 ttgtttgtaa agtaaagcga accaataatg gttgtgtttt tattactcgg attttatcaa
     1081 gcaaaccaac atatttgttc atcacgatca cctcgctaaa attatagcaa aaacagctaa
     1141 aattgagaat ttttattcga ttttagatat gctataatga tcaagacagt aaagtttgtg
     1201 acggtggcta tccgattgaa agtgaggtgg tgcgtatgtg cgtacttcca aaatcttaca
     1261 gaaaggagaa gccttttgcc tgttgaaacg gcgctaggat taatgattag tttcggttct
     1321 ttgatcgcaa caatcatttt tggaatctta gcagttgtaa acaacaatca aaaaaaataa
     1381 ccgtcagctt tagccggtcg acgattattt ttcaaaaata atattaagct ggtcaccgtc
     1441 ttaaacggtg ctgttagggg agttgttagc gcaactcctc ttttcgtatc cattataaca
     1501 actttattga tcttttacaa tcagaatggt aaatcatcat cactaatatc gattgattgt
     1561 ccgccaaatg gattttgtga agcgttcaat ccattgttgt taggcgtagc tacatcccgt
     1621 tgttcaaagg tggtagggct tggactgctg gacgattgat cagcgtgcgc acggctttct
     1681 gtcgctgctt tactttctaa tagttggaaa ttatcggcca caacttctgt cacatacaca
     1741 cgttgtcctt gttggttatc gtaggttcgg gtttgaatcc gtccaacaac gccgatcaaa
     1801 ctgccctttt tagctaatgt cgccat
//
LOCUS       DAZKKW010000206         1821 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.214, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000206 DAZKKW010000000
VERSION     DAZKKW010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1821
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.214"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(79..1119)
                     /locus_tag="ACG8PO_002914"
     CDS             complement(79..1119)
                     /locus_tag="ACG8PO_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HFQ0208158.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESDISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYA
                     SSPSTITKDWLE"
BASE COUNT          626 a          277 c          224 g          694 t
ORIGIN      
        1 gtaatataat tagaataata aaaaattttt aattttaatg ttactccatg aagcaacaat
       61 cactttgtca gtaaagtttc actctaacca gtctttcgta atagtgcttg gggagcttgc
      121 ataattcaaa cgtttttcac ttgatacttt agcagctatt ggtttttcga taaaccaatt
      181 ttttacatag acaaaaatca ggttctggat attttcgact ttttcggttt ttaatttttg
      241 ataggctttt aacagtgtcg tgtataattc ttgttctgca ttgacataat aactatcgag
      301 ttcttcaaaa actatcgaaa tatcactttc tatttcagct tttttctttg cattatgaat
      361 agtctggacg gttttttgag cttcactgta acttgaagaa aatgttttta tcaaatttaa
      421 tactcgttca gggacaaacg tagcaatgct gtcgtttgtc ataattgtct caaaattgtc
      481 taacaacaat tgatcttgta ggcggtcttt ttcagtgtct atattgtgtc tattagtgtc
      541 taatatatta ttattaagtt ctggtatgat tttcataccc tctgatttat caacgttttg
      601 gcacggtatg attttcatac cctcttgagc cccaagggat tcagcgtctg atttttcgtc
      661 gagggtgggt atgattttca taccctctga tttatcaacg ttttccctat tctctctatc
      721 aaatttttcg agtaagtaga tatcattttc tgagactttt aattctgcta gatatagtct
      781 attaggatta tttttcccta actgtttgtt aaatcccatc tgcttttgta ataataaacc
      841 aaaggattcc aactcttttt tgattgctat cgctttttgt ttttggcagt ttaatactct
      901 acatagttca tttgtcgtat aagtaaaata aacattacct tcttcatcga tttgccgttt
      961 gctgattgcg atctctaatc ttgcttttaa aagcatatag gcaatcttag ttgaatcttt
     1021 catttttttg tattcgtcag aaacaataaa gacttttgga aattgataaa ataattcgtt
     1081 gtaaactcta tccgccttaa aaaatttaaa atcagtcatt gtagatcctc tactttcacg
     1141 ttgttttatt tatgtaaaat tacctactct tttcgaaaat agcaaagata aataaaaaat
     1201 gcaaacgcat taaaactgtt ttttaattaa atactaccta aaatcagaaa taaaatgaat
     1261 tatgtactac ctttaaagtc attaagaact actttaaata aatattattt ataattaaat
     1321 actaccagaa aaacgtatga ataaaattta gttttaaaat taaagataat taagtactac
     1381 cagtgtcaaa acaaaaaata aattaagaac taccttatgc atataaaaca tactaccatt
     1441 actagtaata tatactacct tatttcagta gtatatatta tattattctt ataagacaag
     1501 gctttaggct agaaaacaat agattatata ctactaaaaa atacaataag tactaccaat
     1561 gtcagatata tataataatt atgtactacc attaaaatta acttatttaa taagatacta
     1621 ctgtgttaat ctgaaaaaaa ttactttaaa gtagttacat actacctgtt aatttagata
     1681 ctaccattaa tacttaaatt agatactacc tatatttata ttaagtacta ccgttctaag
     1741 aataagataa taaaaaaata ctaccaaatt gcaattaaat actacttaaa atgtaaaaaa
     1801 taagaactac ccaaattagg t
//
LOCUS       DAZKKW010000207         1798 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.215, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000207 DAZKKW010000000
VERSION     DAZKKW010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1798
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.215"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..808
                     /locus_tag="ACG8PO_002915"
     CDS             <1..808
                     /locus_tag="ACG8PO_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HFQ0208159.1"
                     /translation="VHYFFTGKIEDLFHEPLIEYTENLSKTIEQIKIDNLLSQNYDPE
                     IYLFLYENKILEYVKNGDIRNLENMVFNLSNGIIPSVSGDTIRSEKNYSIIVFEKLAQ
                     TSITLGMDIIEAYQSRDALIQENELAVSLPEVLKVRDSGIVYYTKEIGKTKIEHLSPL
                     ISSVVQFIGLNIYKRITVKEIANYFSVSETKLRESFKNEMHITIYNYISKRKISTAKI
                     MLKSNHTISEVSLGLGFSDSSHFSRVFKKYAGVSPKQYQLGLVDNFNNIS"
     gene            825..1259
                     /locus_tag="ACG8PO_002916"
     CDS             825..1259
                     /locus_tag="ACG8PO_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0208160.1"
                     /translation="MRLYHLRSLEELRKLDFNKKFFWEISDLSCFVESERLSNGNNSV
                     LNEYDKVVINKVKSLCEEARESISHINGSNFKQTLFNLIKLDAKISSYLFFLIHDEFM
                     DYQKLDQLIERESWEDYYVGLTFSEKLNNYKLIDYKYLSESN"
BASE COUNT          677 a          208 c          302 g          611 t
ORIGIN      
        1 agttcattat ttttttactg gaaaaataga agatctattc catgaaccgt taattgaata
       61 tacagaaaat ctaagtaaaa ctatcgagca aatcaaaata gataatctgt tatcacaaaa
      121 ttacgatcca gaaatatatc tgtttcttta tgaaaataaa atactggaat atgttaaaaa
      181 tggagatatt cgaaatttag aaaacatggt ttttaatctg agtaatggaa taattccaag
      241 tgttagtgga gatacgattc ggtctgaaaa aaattattct atcattgttt ttgaaaagtt
      301 agcacaaacg tctataacat taggaatgga tataatagaa gcgtatcaaa gtagagatgc
      361 tttgatacaa gaaaacgaat tagctgtatc gttacctgag gtcttaaaag tgagggattc
      421 tgggattgtt tattatacta aggaaatcgg aaaaactaag atagagcatc tttctccctt
      481 aatttcttct gtcgttcaat tcatcggctt aaatatctat aaaagaatta ctgtaaagga
      541 aatagctaat tatttttctg taagtgaaac taaattaaga gaaagcttta agaatgaaat
      601 gcatattacg atttataatt atatctctaa gcgaaaaatt tctacggcta aaataatgtt
      661 gaaatcaaac catactatca gtgaagtttc tttaggttta ggattttcag attcttcaca
      721 cttttcgaga gtattcaaaa agtacgcagg tgtatctcct aagcaatatc aattaggttt
      781 agtggacaat ttcaacaaca ttagctgaga ggttttattt tattatgaga ctatatcatt
      841 tacgtagttt ggaggagttg aggaaattgg attttaacaa aaaatttttt tgggaaattt
      901 ctgatttgag ttgtttcgta gaatctgagc gtctaagtaa tggtaataat agtgtgttaa
      961 atgaatatga caaagtagta attaataaag taaaaagttt atgtgaagaa gctagagaat
     1021 ctatcagtca tattaatggt agcaatttta aacaaacatt atttaatctg attaagctag
     1081 atgctaaaat atcaagttat ttattctttt tgatacatga tgaatttatg gattatcaaa
     1141 aattggatca gttaattgaa agagaaagct gggaagatta ttacgtgggt ctaacttttt
     1201 cagaaaagtt aaacaactac aagttaatag attataagta tttgagtgaa agcaattaat
     1261 tattcatgta gagtactaga cactttaaat atggacaatt caattataaa gaaaaagaat
     1321 caataattta atgaggctgt gacaaatgtc attttttgtt tcgctaaaaa cgagtgaaaa
     1381 aatgttgata actccaatca ttcgtttaag attctaggaa tcattcagaa atgctgtgaa
     1441 accgatactt tataattata aagaaggtga tttcaaattc cgaagaagga atttgaagtc
     1501 acctttttac ttatgtcaca gtctcattca gtaatgcggg ataaggctcc ttattccgtt
     1561 atttgaatac tctgaagttt aatctaagaa agggtaatat tttgaaaaca tgcttcatcg
     1621 catagaaatg aacttaattt cagaaagaat acatacatat ctttttatat gttttcagct
     1681 gctgaatggt taaaaagcac aaatatccga aaaaaacatg aagaaatgta ctttttttgt
     1741 tgtaatgttc atgaaattct ctaagtaaat tagttaacat aaggttagta agattcct
//
LOCUS       DAZKKW010000208         1762 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.217, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000208 DAZKKW010000000
VERSION     DAZKKW010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1762
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.217"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..343
                     /locus_tag="ACG8PO_002917"
     CDS             <1..343
                     /locus_tag="ACG8PO_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0208161.1"
                     /translation="VYLDSNRLYRYLRGQKTNIQIWTAEMWAQLWNMMYFNIGPKVHE
                     ELDFCFATDPIEKVKEVKILHNAGVTTNDEDLFFKGRYVTSTPFDEDLSFVNKKKCSY
                     AYVKAIKAVVR"
     gene            340..651
                     /locus_tag="ACG8PO_002918"
     CDS             340..651
                     /locus_tag="ACG8PO_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0208162.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            641..994
                     /locus_tag="ACG8PO_002919"
     CDS             641..994
                     /locus_tag="ACG8PO_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFQ0208163.1"
                     /translation="MANNRRLEETFNDGWLKILTQTTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIYADYDN
                     FYIYFYLESVGEYGD"
     gene            984..1385
                     /locus_tag="ACG8PO_002920"
     CDS             984..1385
                     /locus_tag="ACG8PO_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0208164.1"
                     /translation="MVIDNSKEKERLNKQISAIKTSLEEHFGLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQNLYLTFYSENREDLTGDSLDIISLIQNRSIRFQRM
                     DPNHLKLENQDRYIDQLVFTFRRLLKSDCHG"
     gene            1378..>1762
                     /locus_tag="ACG8PO_002921"
     CDS             1378..>1762
                     /locus_tag="ACG8PO_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0208165.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQ"
BASE COUNT          635 a          261 c          348 g          518 t
ORIGIN      
        1 ggtttatctg gattctaatc ggctatatcg ctaccttaga gggcaaaaaa caaatataca
       61 aatctggaca gccgagatgt gggcacagct ttggaacatg atgtatttca atattggtcc
      121 taaagttcac gaggaattag acttttgttt tgctactgat ccaatagaaa aagttaaaga
      181 agtaaaaatc ttacacaatg ctggagtaac aacaaacgat gaagatttat ttttcaaagg
      241 gagatacgtg acttctacgc cttttgatga agatttatca tttgtaaaca agaaaaaatg
      301 ctcttacgca tatgttaaag caattaaggc ggtggttaga tgacgcctga acaagtgact
      361 gaagaattgc taatagctgt gaaggataat atttacgtta cctggaacga agaagatgag
      421 tcaattaaaa agatgatagc taaaaatgct gtttatcttc aaagtaaagt gagtacaaca
      481 ctttcttttt ctcctgaaag cttagaatac ggattgctaa tcgaaagatg tagatacgac
      541 tggaatcgtg ctttagatga gtttgaacaa aatttcgcta gtgagttatt aggtttcatt
      601 caacattatg cgctacaaga atatattgca ggtgatgtga atggcgaata atcgtagact
      661 cgaagaaaca ttcaacgatg gttggttaaa gattttgacg caaactacca aaagaaatga
      721 actaggaaaa aagattggtg tagaagatac agaaatcact tctttaaaat ttagaaatct
      781 ttccatgaga gatagtgata taacagctat ggatgcgatg ggatcgaaat taactaagaa
      841 agtaaagact ccatttcatc caatcgccaa gaaatttaat aaagatcaat attttatcgt
      901 aatcgatagt atgcgttaca acgttatcta tgccgattac gataattttt atatctattt
      961 ttatcttgaa agtgtgggtg aatatggtga ttgataattc taaagaaaaa gaacgtttaa
     1021 ataagcaaat ttctgctatc aaaacttcct tagaagagca ttttggcctc aaactctttc
     1081 aagactccgt aggcgaggat gagctacctg atgattttaa ttacttcatt ctcgaaacag
     1141 gagaaataga aatgatcact gagccaaaat atagcgtggg tcaaaatcta tatctaactt
     1201 tctattcaga aaatagagaa gatttaacag gagactcact agatattatt tcattgattc
     1261 aaaatcgttc gattcgtttt cagagaatgg atcccaatca tttaaaacta gagaaccaag
     1321 atcgctatat cgatcaattg gtatttacgt ttagacgatt attgaagagt gattgtcatg
     1381 gctaaaaata gttgggagct aaaaataaat ggacatgatg aacttcttgt gcggatggaa
     1441 cgctattcaa gcgagagcga acgactgatt aacgaagcat tgaaatcaaa aggttcggct
     1501 attgcagtgg ataggattac agaaaaaatt cctgtttctg aagcagattt aagaagaggg
     1561 caccaacacg caaaaaatag tcgtccactt aagactcaat acattaattt gggtttcatc
     1621 attagaccta caagaaaatt tgagtattta aaatatcctg atttggggat aggtacttct
     1681 aaaagaaatc agccggacga atttatgaga agaggattag gtcttgcact tgatccaatt
     1741 acagaacttc tgattcgtca at
//
LOCUS       DAZKKW010000209         1735 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.218, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000209 DAZKKW010000000
VERSION     DAZKKW010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1735
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.218"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(27..1508)
                     /locus_tag="ACG8PO_002922"
     CDS             complement(27..1508)
                     /locus_tag="ACG8PO_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0208166.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
BASE COUNT          540 a          233 c          300 g          662 t
ORIGIN      
        1 aataagagga tgtgacaaga attttgtcac atcctctttg ttttgttctc ataacctttt
       61 ttcatataca gttcatgaac cgcttttatt aacttgccaa ataacactgt attgtgcgtt
      121 gtatccagat agaaagtctc cgtttcatct gaaattttct catgctgatc tgtaatgatc
      181 aaatcagcat gttctgaata tgcggtaatt ttaacagcat ctttgcgaaa taactgctgc
      241 aaatggcttt gaagatgata atttcctgta aactgtttgg ttatctgcaa atggatgagt
      301 aactgctctt tcgtttctgt ttgtagtaag tgttggatca attgactaag gtattgactg
      361 atttggtttc tttccccatt ttttgaaaaa attgtatcga ttttttctct taatttcttt
      421 tcctgtataa aagaactaga atgattggac gtggcttctg gaaagcaaag tgtagaaaaa
      481 ctactgctgg attcacccaa cagttcgaaa aaaacaactg ccaacacaag aaaataaaaa
      541 taaattggta ttttattttc taaatggttg agaggaaact gttgagttaa gtaagtcatc
      601 aattgaccga cttgtcgaca aataaggttt ttgcttgtgg aaaataaaaa accaatctcc
      661 tgtttttgtc gaaaagagat ttgatttgga aaaaaacaat agaaaaaata attaaagtag
      721 gctctctctg tttctgtctc ttcacaaggg cgactcactg aaaaagaagt aaagtattct
      781 gaataatcat gtgtatgctg caataagtcc atcaattcag tgatttcaga agaagttttt
      841 ggaaaatatg cctgttgttt gtttcgtaat atgaataaag ttaacagcaa gtagatattt
      901 ttttcttgtt ttgaaatttt ttctatttgc tcatctgtac tgttcatttt tagtgcattt
      961 aattcaagtg aggaaaatgg ccaatcgtta cctccaaatg tatctgaaag aaaaaagtaa
     1021 agatagagac ggatagataa ctcatttcct tcaagagtac agcaattttt tacagatagg
     1081 atccgtaaat ggaatttttt taaggaatga ttgagctgtt ttgtcagttt ttcgagatag
     1141 gagggcgata tataaagttg ttcaagtaaa tatttttttg gtacagcagt ttgtggataa
     1201 ttgattagta acatcaaata gttaaaacga ttggattctt ttaggtagca taattttaac
     1261 ttagaaaaaa cagctaactt tttagtttga tcggtttctt tggtttggat ctctccttta
     1321 tgattagaat aaataaattc atcaatcaat aaaaattgct tgagtgatac atttattttt
     1381 cggatcagtt ctttgattgt ccgttttgga atatgtaagt cattggataa ttcttgtaaa
     1441 gaacaattat ttttttgaat gaccatttgt agaacaagta actctttttt tcttgcttca
     1501 attagcataa atacctcctt tttattctgt ttttgtatat agttagacaa aaactgtgag
     1561 tggaaagtca tttcagtctg tgttattctc acagagtgtc gccttctttt ttatataacg
     1621 ttatttaatt tttttgttac cttgtttatt ctattgttta tttcctaggg tattttgtca
     1681 agatattctt gtttttaagg cggaaaaatt gatttttaat ggatataact aaata
//
LOCUS       DAZKKW010000210         1642 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.219, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000210 DAZKKW010000000
VERSION     DAZKKW010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1642
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.219"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(96..437)
                     /locus_tag="ACG8PO_002923"
     CDS             complement(96..437)
                     /locus_tag="ACG8PO_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
                     /protein_id="HFQ0208167.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSF
                     EIIEFVADKEE"
     gene            complement(1053..1385)
                     /gene="traE"
                     /locus_tag="ACG8PO_002924"
     CDS             complement(1053..1385)
                     /gene="traE"
                     /locus_tag="ACG8PO_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraE"
                     /protein_id="HFQ0208168.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
BASE COUNT          486 a          361 c          216 g          579 t
ORIGIN      
        1 aaattcctta ctcaatgcct aaggtaatac taccttttta ttaatcgtcg tgactccttc
       61 taagccttta tctgtaaaat ttattcctcc agtttttatt cttctttatc cgctacaaat
      121 tcaatgattt caaagctgac aatataatca ctcaaatatg accaagtatc ttcaacagat
      181 aattcgtctc cattatcatt cataagcgtc attttaaacc attcattacc ttcatcatct
      241 tcaggtagca agccgaatcc aattggatta tcagtcaatg tttgatcgta taaatcatcc
      301 tcgtctaatg ctaaaacatc taatccttta caatttccgc cccttctaaa cgtcacaaat
      361 cctttgtaat cccaacgttg gaaagaaacc ttaatagtat gtgtgcccaa tcgtatattc
      421 ggatcataat ttttcatttg tcattcctcc attacgtaaa tttcattttc tctatgcttt
      481 atattaattt tttcacttta ttcaggactt gttcatattt tatcgttttt cgtttactta
      541 aaaatacata aaaaatttcg actcgtttat ggcgtccgaa acgagaaacc ctagacagcg
      601 gtaaatgctc gattgatcca ataatcaacg tcctcgcaag tatctacatg tactgcagca
      661 cacgacacaa tttcttgtgc gtcctcccca ttacgagaag acctactcct tattccatga
      721 gtttgcgtca aagtctagat cccttagtag ctgctcatga ttaatctaga cgctattcta
      781 acttgtctaa cttctaaatt ccctcttgtt tactctgcgc ttattattta cacaaattca
      841 tttcaccact atccaaacaa gcgcatttac tcgattgacc aattttttac gttaccaatc
      901 gttaaaacct accccgtcac tccccgaagg gaattgttga tagatcaagt ctactcctta
      961 tttttctagt gactctaagt tcctttagaa gtctcgacaa tattttcaag caagcgaaaa
     1021 acgataaaat acaagttatg agaatgatca attcagccga tcaaacgccc catccctttt
     1081 gcggtacaac gcaagactcc attttttctt cgaccaatca gtacaccgtt ttggtcataa
     1141 acatgtttga ttgttggata tagctctttt aaggggatag gatcttttaa aattatttgc
     1201 ttttgtttcc caatgtaata aatcatttcc tctgagactt tgtctttctg gattatatat
     1261 ttcttacact gcacaacggt tttactttgg ccagttgata gtttcttcgt gattttctct
     1321 gtagcggtac accgtttacc gtagtgacaa atatctttga gactcaaaat tcctgtatat
     1381 gacataaaat aaaccaccca atttctttaa atatttttaa caagtttttc ttgttatttt
     1441 tcaaaaatag agaattgatt ttcgttcatg acaaacaaaa aagagggaac gcatccaaac
     1501 atgcgttccc tctaatttca gcgttcgtca tacggcaaag agccacacgg acatttttat
     1561 aacattatgc ttaggcatag attcatcgta atatcacgta tttttaacct taacacagcc
     1621 aaaaaaagtg gtatcacttt tg
//
LOCUS       DAZKKW010000211         1634 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.220, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000211 DAZKKW010000000
VERSION     DAZKKW010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1634
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.220"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            36..1076
                     /locus_tag="ACG8PO_002925"
     CDS             36..1076
                     /locus_tag="ACG8PO_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HFQ0208169.1"
                     /translation="MNDFNYYKSKEIYREKYYQMPKVFFTNEKYMDLSNDAKIAYMLL
                     KDRFDYSVKNNWVDSDDNIFFIFTVEELMKLLQCREGKVSKIKKELEKAGLLKQKRGR
                     VNKRDGKIESMPNLLYLGKPEVTNQDVFKIMEEEDNTDTTVIAKIANTAKPREINDTT
                     VIAKIANTAKSSHIKDSTVIAKIADNLFYSNSIDTNRHIIDTETDQLQNQVLLDNFVD
                     IMKEDSINTFVPENVLNLIKTFSSTYEDAQKTVQTIHNAKKKAEELEQTVVVYEELES
                     YGIDADKGLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWFVEKAIAAKLANEQRETFN
                     ELPTVSTENWLE"
BASE COUNT          631 a          239 c          276 g          488 t
ORIGIN      
        1 gaacaaaacg ctatcctaca gaaagaggat tggctatgaa tgattttaat tattacaaat
       61 caaaagaaat ttatcgtgaa aaatattatc aaatgcctaa ggtatttttt actaacgaaa
      121 aatacatgga tctatcaaat gatgcaaaaa ttgcttatat gctacttaaa gatcgatttg
      181 attattcagt caaaaataac tgggtagatt ctgatgataa tatatttttc atattcacag
      241 ttgaagaatt aatgaaattg ttacaatgcc gcgaaggtaa agtatctaaa atcaaaaagg
      301 aattagaaaa agcaggtctt ctgaaacaga agagaggaag agtaaataaa cgtgacggta
      361 agattgaatc catgcctaat ttattgtact taggcaagcc agaagtaacg aatcaagacg
      421 tctttaaaat catggaagag gaagacaata ctgataccac cgtaattgcg aaaatcgcaa
      481 atacggcaaa accgcgagaa atcaatgata ccaccgtaat tgcgaaaatc gcaaatacgg
      541 caaaatccag tcatatcaag gactcaaccg taattgcgaa aatcgcagac aatctattct
      601 attctaattc tatagacact aatagacaca taatagacac tgaaactgat cagttacaaa
      661 atcaagtttt actagataac ttcgtcgaca tcatgaaaga ggacagtata aatacatttg
      721 taccagaaaa tgtattaaac ttgataaaaa cattttctag tacttacgaa gacgctcaaa
      781 aaaccgtcca aactattcat aatgcaaaga aaaaagctga agaattagag caaacagttg
      841 ttgtttatga agaactggaa tcttatggaa ttgatgctga caaaggatta tatatgactt
      901 tgctgaaagc ttatcaaaaa caaaaaactg aaaaagtaaa caatttgcag aatctaattt
      961 tcgtttatgt aaaaaattgg tttgtcgaaa aagctattgc ggcaaagctt gcaaacgaac
     1021 aaagagaaac atttaacgaa ttaccaacag ttagtacgga aaattggcta gagtagttga
     1081 taaaaagcga tcactataaa aatgatcaat tttaaattag aactttttta tgattttaat
     1141 tttattacaa atgttacaaa taaatagcta ttattccgga gtttttagat taaaagttta
     1201 tgaaaacaat cacaaacgta ggttttaaag tcataatcgt tagatatttt agttattttt
     1261 cattcaaaaa aatagggaac tagtgtttgc atatgtgctt ttttgagtta taataattca
     1321 tgaagagagg taattcactt atttccccaa gctgaaaagc ctgatattac ttcttttcag
     1381 gcagtgctgc taatattcaa ttacactttt ttgtatattt ttagttgttt taaatttaat
     1441 tgtctgatat gcttgtgatg aattgatttt cgttctgcac ttttggacga taaattgtga
     1501 taattgaaaa tacaaaaaaa agaaaggccc ctgtaaggct cgggagactg gtaatctccg
     1561 tcagtgtcct acaggaaagc attaagatga cgccttaatg cgtgcccttc agtgttacgg
     1621 tattacccgc aacg
//
LOCUS       DAZKKW010000212         1598 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.221, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000212 DAZKKW010000000
VERSION     DAZKKW010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1598
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.221"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            46..483
                     /locus_tag="ACG8PO_002926"
     CDS             46..483
                     /locus_tag="ACG8PO_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0208170.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKEKLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNGKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYAKFAQIKKAKAMASDWLDTFENIEKNGAFYTIESEEKDNE"
     gene            476..1183
                     /locus_tag="ACG8PO_002927"
     CDS             476..1183
                     /locus_tag="ACG8PO_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0208171.1"
                     /translation="MNKVVYQLTTEDVSQFLTNKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYMESETSQTKVTVSHNRLDVYKRRPDTLKKGAELLRLETEKYIMNVYLERS
                     ELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKDATVDDYYLVFTRFLT
                     CGTESIVLMGMDYELTVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKGQ"
     gene            1348..1548
                     /locus_tag="ACG8PO_002928"
     CDS             1348..1548
                     /locus_tag="ACG8PO_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0208172.1"
                     /translation="MNKIIPLLFVLFVGEILMRHGAALHLNFVVFLGILVFCLIFWRF
                     VLTLVSLIFKLIAAIFLIGLFR"
BASE COUNT          587 a          255 c          336 g          420 t
ORIGIN      
        1 cgaatattgg ttaaccaaat tagagaacta aaaaggagaa aaaatatggc tcaaaattac
       61 tatgacgaat ttgtcaaatt acctttggat aaaatggcac aaaaaatgga ggatatgacg
      121 ttcctatacc atgaaacacg tgtacccaaa aaacattaca aggaaaaatt gtcagtcgca
      181 gtggaagaaa tgattgaatc aggtgtcgaa atgaatctca ttgcgacgta ctatcgaaca
      241 ctggaagaac tgaaaaaaca aaatggaaaa tggtttttcc aagccttact atgcttggaa
      301 gtaggtgtca aaccaagcac tatcaaacct tccgaatatc aagccttaga actgacttat
      361 gcaaaattcg cacagattaa gaaagctaaa gcaatggcta gcgactggct agatactttt
      421 gaaaatatcg aaaaaaatgg tgcattttat acgattgaat cggaggagaa agataatgaa
      481 taaggtggtt tatcagctaa caacagaaga tgtttcgcaa tttttaacaa acaaagaaca
      541 cttgaatgaa gtaatcagac ggattcaaaa agactttagt tgtatggaag aaatgaagta
      601 ctttattcaa agagtggaag aacagttgta tatggaaagt gaaacgagtc aaacgaaggt
      661 tacagtcagc cataatcgtt tagacgtgta taaacgtcgt cctgacacat taaaaaaagg
      721 agcggagctt ctacgactag aaacagaaaa atatatcatg aatgtttacc tagaaaggag
      781 tgagctggtc gaaaactttg tcagctacac gatttgcagg aaacactatt actcagaatc
      841 agaatattat tcaatttttg agagcatgga attaatggaa gaagaaatgg aagagctaaa
      901 cagcaaagat gcgacggtag acgattatta ccttgtattt actagatttt tgacgtgtgg
      961 gacggaatct atcgtgttaa tgggaatgga ttatgaactc actgtacccg aagcattttt
     1021 gagggaatat gtagaagaag tagataaccc aggcgacgac tggtacacga ctttaactga
     1081 ttttttagac acctacactt acgacgatac agaagaaatc caaaatgaac ttgaccgtaa
     1141 acgaatgcca tatcaacgat ttgaaaggaa aaaaggtcaa taaccattta taccgaaaag
     1201 tgaggatgtg tgaaataaga acaaaaaacc ttgaaattaa gggaatggtt cttatttcgc
     1261 accaatcgct ccaaccgccc ccgccgctta ggtggtgcga aataagcggg caaagcccga
     1321 taacctaaga gaaagaagga gatccatatg aataaaatca ttcctctatt gttcgtactg
     1381 tttgttggcg agatacttat gcgtcatggt gcagctctac atttaaactt cgtagtattc
     1441 ttaggaatac tggtattttg tttgatcttt tggcgctttg tgttaaccct tgtgagtcta
     1501 atattcaagt tgattgcagc catattttta atagggttat ttcgatgaaa ggagagagta
     1561 ctaatgccaa ttgcaatagg aaataaacgg ttacccgt
//
LOCUS       DAZKKW010000213         1557 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.222, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000213 DAZKKW010000000
VERSION     DAZKKW010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1557
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.222"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..706
                     /locus_tag="ACG8PO_002929"
     CDS             <1..706
                     /locus_tag="ACG8PO_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFQ0208173.1"
                     /translation="ELTVKYQNELNLVPLKNFNAKQMDLFFALCARMKDRGLKKVRFS
                     FEELKELSDYKMTATKAFVGDLEKLYKDMLNLTYRTEDEDSIRYFVLFTGFEINKTKK
                     FVEVSVNPDLEYIINGLTAEFSRFELSAFTSLRSTYAKTLFRLLMQYRSTGYYVVRIE
                     EFRSLMDIPEYYQMGNIDQKVLNPALRELKKYFNDLKVTKIKAKKGNKIAKLEFTFSS
                     LTSDLPKISLRNWLEE"
     gene            complement(843..1259)
                     /locus_tag="ACG8PO_002930"
     CDS             complement(843..1259)
                     /locus_tag="ACG8PO_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0208174.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
     gene            complement(1262..1546)
                     /locus_tag="ACG8PO_002931"
     CDS             complement(1262..1546)
                     /locus_tag="ACG8PO_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0208175.1"
                     /translation="MDILVRNIDPKFVATIDKLCQELTNKTGEKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
BASE COUNT          490 a          234 c          274 g          559 t
ORIGIN      
        1 ggaattaaca gtcaaatatc aaaatgaatt gaatttagta ccgctgaaaa atttcaatgc
       61 taagcagatg gatttatttt ttgcattatg cgctcgtatg aaagatcgtg gtttgaaaaa
      121 agttagattt tctttcgagg aattaaaaga gttaagtgat tataagatga ctgctacaaa
      181 agcctttgtc ggtgatttag aaaagctcta taaagatatg cttaatctta cttaccgtac
      241 tgaagatgag gattctattc gctactttgt tttgtttact ggttttgaaa tcaataaaac
      301 aaaaaagttt gttgaagtca gtgttaatcc ggacctagaa tatattatta atggtttaac
      361 agctgaattt agtagatttg aattatctgc tttcacgtcg ttgcgttcta cgtatgcaaa
      421 aactctattc cgactgctta tgcagtaccg ttctactggt tattatgtag ttcgtattga
      481 agagtttagg agcttaatgg atatcccaga atactatcaa atgggtaaca ttgatcaaaa
      541 agtattaaat cctgcattac gagaactaaa aaaatacttc aatgatctta aagtcacaaa
      601 aataaaagca aaaaagggca ataaaatcgc caaattagaa tttacttttt caagcctaac
      661 gtcagatttg ccaaagattt ctctgcgtaa ctggttagaa gaataaataa aaaagaactc
      721 gcccccctta cattaagcaa gtagacaagt cccttacaac cgactaagat tgctagtaca
      781 ttctagcata ttgtatgggg cttctaaata aaaaacagat aggaataact cctatctgtt
      841 ttttatcgtt ttagaaaatt tgttgggtcg aaatttctag aagtgactgt ttgactggtt
      901 tctttttgat gattctttgg tcgatttcga tttgatggat ttagtttagc ttcgattttt
      961 tctttcactt cttgatatac tttagaattt gtgccaataa gaacgcctgt atcttttaga
     1021 tataaatcag caaattctgc gatgatttca gtcatgatgg aaatttcttt tcccatagca
     1081 tttagttttg cctgattgct ggtagtggtc gtgttggtat cgggttcata actcatacat
     1141 aaaaagcgaa tggcttccga gtagttattg atatctggat ttttttcgat gatggaatct
     1201 aaaacgtcaa catgactagg gtacaaagta acatgttttt ggacagaagt ttttctcatg
     1261 gttatatcat tcctccgata agcatttcaa tgatttgatt atttgtgtag acatactcgc
     1321 ttaatatttg agtattttct gtttggatag caataaattt atcgagcaat aaatcgaatt
     1381 ggtctttttt gtaagtcgta agttgacggt caaaatcact ttcgattaaa acttttagat
     1441 atttatttcg actccatttt tctcctgttt tattcgtgag ttcttggcaa agtttgtcta
     1501 ttgttgcaac aaactttgga tcaatattcc gaactaaaat gtccataaaa aatctcc
//
LOCUS       DAZKKW010000214         1545 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.223, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000214 DAZKKW010000000
VERSION     DAZKKW010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1545
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.223"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            656..742
                     /locus_tag="ACG8PO_002932"
     CDS             656..742
                     /locus_tag="ACG8PO_002932"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HFQ0208176.1"
                     /translation="MFTNFICSLLVGLLSALFSYWLNNRNKK"
     gene            complement(1044..1268)
                     /locus_tag="ACG8PO_002933"
     CDS             complement(1044..1268)
                     /locus_tag="ACG8PO_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0208177.1"
                     /translation="MEKFPYEYFQNLEKLEQAIQAIKFDLTDEKLLEYKNMTPERKRS
                     LEKLMQLVNEDGGISDAAFDAWSEFHGEYN"
BASE COUNT          464 a          314 c          250 g          517 t
ORIGIN      
        1 ccgttttttc atccattcct ttcttccccc cttacaaata aataataacc tttaacaacc
       61 aactcgtaaa taggttggtt gttattatac aaaaggatat tagtactcag ataaaaagtt
      121 gttagtactc agataaaaag ttgttagtac tcagataaaa agttgttagt acccttctgt
      181 atccgttggg agagtaaggc tcaactgccc cctaaaaagg gttctaaaaa atctttaaaa
      241 aataaataaa ataaacgcgc gcgcgcgagc aaaatttccc gccaaaaaat cacccacttt
      301 ttaaaataaa caggttcgcg catgatatta aactgccaat ctagctccct atggtcgctg
      361 taagaaggag tgatcgctac gctctcacat tttacttgca gcagtcgcct ttaggctcct
      421 tcgccaaaaa taattcatta gggactcctt tgtggcgtcc tgagagctcg tctaagccgt
      481 tctaagacaa gattcgataa aaactatgtc cttcgttcta aagcgcctta caggcggtca
      541 gggcaagccc taacaacccc aaggcaataa aaaacttagg cttatttttg cttttttatt
      601 gtataattga ttgtcgccta tccgacagtc ttgcgactgg aaggaggtgt gtttcgtgtt
      661 cactaacttt atttgctccc ttcttgttgg gcttttaagc gctttgttca gttactggtt
      721 gaataaccga aacaagaagt aaaacttaat taaggcgact cagcccacaa aaaaagcccc
      781 agagaatccg acctctctgg ggatttcgtg tgtttcgtgt tcacttattt gcaatcaaat
      841 tataacagaa ataaatattt atttcaaaaa tatcctacaa aagtctaaaa tccgtaatta
      901 tttcttatat gcaacattta caaataaagt cgctctgaga gctcgtattt ttcgatttaa
      961 gacgttttat ttagtttttg gtgtatttca ttggtccaag ctaaaaaact cttagaagcg
     1021 attttagagc catttaaacc cacttaatta tattctccat gaaattcgct ccatgcgtca
     1081 aaagctgcgt ctgaaattcc gccgtcttcg ttcactagtt gcattaattt ttccaacgat
     1141 cgttttcttt cgggtgtcat atttttatat tccaacaatt tttcgtctgt taaatcgaac
     1201 ttgattgctt gaattgcttg ctctaatttt tctaagtttt gaaaatattc gtaaggaaat
     1261 ttttccattt tttctttctc cagtctcaca atttttctag aaataaaatc acttctttgc
     1321 cgtctatact ctcaaaaaaa atgtcgtttg aaaaactaaa taaatttttg atcgtgcatt
     1381 tttaaatttg gttatcgatt acacgtgcag cactcccccc cgaattttaa agagcataag
     1441 gaaacgtgta cccactttct aaaaaaagtg ggtagatagg ctgtgcctat cacgtttctc
     1501 agaaaaaaat ttgatatcac tttttcgcca tcgtgggtag cttat
//
LOCUS       DAZKKW010000215         1543 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.224, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000215 DAZKKW010000000
VERSION     DAZKKW010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1543
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.224"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..200
                     /locus_tag="ACG8PO_002934"
     CDS             <1..200
                     /locus_tag="ACG8PO_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFQ0208178.1"
                     /translation="IEDSLLINLYYSNKNYFKIPAAKTRMKKGVYFLFDIVTDVPDAR
                     SDHRRYDYIKYTFVDPERYKD"
     gene            complement(236..607)
                     /locus_tag="ACG8PO_002935"
     CDS             complement(236..607)
                     /locus_tag="ACG8PO_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0208179.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKLT"
     gene            complement(609..1010)
                     /locus_tag="ACG8PO_002936"
     CDS             complement(609..1010)
                     /locus_tag="ACG8PO_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0208180.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            complement(1024..1431)
                     /locus_tag="ACG8PO_002937"
     CDS             complement(1024..1431)
                     /locus_tag="ACG8PO_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0208181.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
BASE COUNT          495 a          269 c          204 g          575 t
ORIGIN      
        1 caatcgaaga tagcttatta atcaatctct actattctaa taagaactat ttcaaaattc
       61 ctgctgcgaa aactagaatg aagaagggtg tatacttttt gtttgatatc gtgactgacg
      121 tgccggacgc acgtagcgat catcggcgtt atgactatat aaagtatact ttcgttgatc
      181 cagaaagata caaagattaa ataaaaaaat aacaaacaaa ctcgcttgaa attcattagg
      241 tgagtttgtt tattattttt tctgcttctc gattcatcag cgccatatct gttttcgtaa
      301 tggtttcatt aggaatatag tcagcacatt ttcttttctc taacattcta tacccactat
      361 tatgtattcc taaggtattc aattccttaa ctgttgaaaa caaattccaa acggtttggt
      421 ggcttccttt tccgctactt ccattataaa taatatcata attttctttc aaatactcat
      481 cagatttttt aaaagcactg tagtatgctc ttgaaacagc agttcttcta taagattctt
      541 tagaattata tttatcatct gattcaattt gtttggaaat agataaatat tcattaaagt
      601 caaacatttt attcccccaa agttgctact acagatttcc ccatatattt atagtcagat
      661 aatacattat cgagagtatc caaaacatcg taatctgagg aaaacaattc agatgttgtt
      721 ttgataacaa agaaatacaa gtttaaatct tcatcatatt ttatattgat ttcagctttt
      781 ctttttaaaa ccgtatttat cacatcaaaa attttgccat cattacttga aattgtgtta
      841 atctcatttt ttgtagtaat tacaagttga ttatcatttt tagttttcgg agtagaactt
      901 gcatccacaa caactggaga tgctagaatt accgatgcgc aagcaaaaat cgcagcaaaa
      961 tttattaagc taccactccg atgtgtattt gatggtacta tatcaagcat ccgtatcgtc
     1021 tccttaattt ttttccactg gtttgttgac cttgatatca aagatttgcc ttccaatctc
     1081 agttccatta aatttaacaa cagcataatg ctctcctgct tcttcaacca agacatttct
     1141 ggcgtttata ttaaagttta tatcgttaac ttgaggaggc aagcttaccg atacttcacc
     1201 cgtagaaaaa atttgttttt cattcttgtt taaaacttca atttctacta tttttatctt
     1261 ttctgtatcc tctatctcag acaccacagc tgttacagaa aatgataaag cagtaggaat
     1321 taaaggcgca ttaatagaaa gagctggatt aattacttta ttaataacat tagtgccaaa
     1381 ttgctgactt tctacatctt ctgaaattat taattgtact ctataagaca ttgatttctc
     1441 ctaattttta catttattta ataaatttat gtttcacttt tgctagtcaa tttagtttaa
     1501 caatagtaac aaagctttgt caacggaaat attagctaat ttt
//
LOCUS       DAZKKW010000216         1526 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.225, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000216 DAZKKW010000000
VERSION     DAZKKW010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1526
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.225"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..318
                     /gene="ssb"
                     /locus_tag="ACG8PO_002938"
     CDS             <1..318
                     /gene="ssb"
                     /locus_tag="ACG8PO_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFQ0208182.1"
                     /translation="MVTLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQLLE
                     SKAATESRAHADQSSTSPSTTTFEQRDTATPNNNGLNALQNPFGNPFGGQSIDISDDD
                     LPF"
     gene            684..944
                     /locus_tag="ACG8PO_002939"
     CDS             684..944
                     /locus_tag="ACG8PO_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0208183.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDHFTRSMGI"
     gene            1389..>1526
                     /locus_tag="ACG8PO_002940"
     CDS             1389..>1526
                     /locus_tag="ACG8PO_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HFQ0208184.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGQRAKCIKSYRLYQC
                     PT"
BASE COUNT          499 a          297 c          306 g          424 t
ORIGIN      
        1 atggtgacat tagctaaaaa aggtagcttg attggtgtag ttgggcggat tcaaacgcgg
       61 acttatgaca accagcaagg acaacgtgtg tatgtgacag aagttgtggc cgataatttc
      121 caactattag aaagcaaagc agcgacagaa agccgtgcgc acgctgatca atcgtccacc
      181 agtccaagca ccaccacttt tgaacaacgg gatacagcga cgcctaataa caatggattg
      241 aacgctttac agaatccatt tgggaatcca tttggtggac aatcaatcga tattagcgat
      301 gatgatctac cgttctaagt agcattttct attggttgca tttgctacgg tttatgttat
      361 aatttagtca acaaaaagaa ttttcaatct ataaccaaaa accactagct ggcgggctag
      421 tggttttctt ttacgtttct attataagaa ttaacgggta ggcttactgt ctttcttggc
      481 aaatttacaa tcaagccatt tgcagatata gtgcgcggct acttctgcca tgattgcaaa
      541 aacaaaatca aaaagaattt tcaaacctat aacctcctcc ccttgccaga ttaggccggg
      601 gtagcagaca gcaagactat tatagcataa ttgaaatatc caaatgattg gatttttgct
      661 ataattttag cgaggtgatt gtgatgaaca aatatgttgg tttgcttgat aaaatccgag
      721 taataaaaac gcaaccgtta ttggttcgct ttactttaca aacgattcat gaatcgatta
      781 attgtgtagt tgcagatatt gaaattatcg ataaattgct aattatggat gatggaaaat
      841 acaatattgc cgtcacaggt cacttcaata aacgaaatca gctagttatt gctagcatgt
      901 atgttagaaa tcccgatcat tttacgcgat cgatgggcat ataaaaaaag aagagttgca
      961 atcctaatgt aggactgcag ctctttttgt tttacataga aacaagaaaa atgaaaaggc
     1021 ctttttagga aggcgcgagc gccttctcaa ttgaaaaacc tctctcactc caaccgccac
     1081 cgccgttcga gaagaaagga agtgaacgtt gcacgttcac taagataaag ggtcttgcaa
     1141 agcaagacaa aatagaaaaa acttcctctc accaaaaggt tcgttcctgt tcgttatctc
     1201 tttattgatt tgccagaccg gcaacaagtt gccttttatc accaaaaagc gacaaggaca
     1261 agggtaaatg gcacgctgcg cgcccttgtc cttgtcgctt ttcgttgatt cattctggcc
     1321 aacaaatcaa taaagaaaga actaacgaaa ggaagtaaaa cctttcctaa gaggaggcag
     1381 aatcaatcat gcgaaagaaa tttgattatt ggggtgttcc cttcagcgaa ttattcccaa
     1441 actatcatgc accacatact gtcgaatgcg attgtggaca aagagcgaaa tgtatcaaaa
     1501 gctatcgttt gtatcaatgt ccaaca
//
LOCUS       DAZKKW010000217         1525 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.226, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000217 DAZKKW010000000
VERSION     DAZKKW010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1525
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.226"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            12..1478
                     /locus_tag="ACG8PO_002941"
     CDS             12..1478
                     /locus_tag="ACG8PO_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HFQ0208185.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
BASE COUNT          417 a          289 c          324 g          495 t
ORIGIN      
        1 gggtatagat tatggaaaaa cagcaagaca agggcttctt cgggcagccg aagggattgt
       61 caactttgtt cttcactgag atgtgggaac gatttagtta ttacggtatg cgtgcattac
      121 ttatctatta tatgtacgat acagttgcca acgggggact tggattacca cgagcaacag
      181 cattagctat catgtcgatc tacggatctt tgatctacat gtctagtatc gtcggcggat
      241 gggtatctga ccgagtgtta ggagcaaaac gcactgtatt cttcggtgga gttttcatta
      301 tgcttggcca catcgtcttg gctttaccat ttggtgtaag tacattgttt gtttcgatgg
      361 cattgattat tatcggaacc gcctttttga aaccaaatgt ttctggcatg gtcggacatc
      421 tttattcaaa gactgacttg cgaagagatg caggattttc gattttttac atgggtgtca
      481 acttaggggc attattagca ccactaatcg ttggtacaat cggtcaagaa tataactatc
      541 atttagggtt ctctttagct gctattggga tgttctttgg attattacaa tacatcatcc
      601 aaggacgcaa gacattagac ggagtgggta caacaccgcc gaatcctttg acaactgaag
      661 aacgtaaagg attcatccgt aacttgatca tcgcacttgt tgttatcgct gcagtattcg
      721 gcggggcaca actaactggt catctgacaa ttgacttttt tgtcaataca atcagtgtat
      781 tggggattat cttaccgttg tattacttta tcaaaatgct gacttcaaaa gatgtaactg
      841 ctgaagaaaa accaagagta tgggcttata tcccattatt ccttgcagca atcattttct
      901 ggtcattaca agaacaaggt tcatctatct tagctttatt tgctagtgaa cgtacacaat
      961 ctacgctatt tggtgtaacg atccaagcaa gctggtatca atcattgaat ccaattttca
     1021 tctttattct gacaccagta ttcgtaacga tgtggacgaa acttggtgaa cgtcaaccat
     1081 ctacagtagt gaaattctct cttggacttt tatttgcagg attatcgtac ttgctattga
     1141 tgttgccggg tatgctttat ggtacaagtg gaaaagtaag tccattgtgg ttgttgggaa
     1201 gtttcttcat cgtggttatt gctgaattgt gtctttcacc agttggtttg tctgtgacaa
     1261 caaaattagc accacgagca tttgaatcac aaacaatcgc tatttggttc ttagctgatg
     1321 cttcttcaca agcaatcaat gcacaaattg cacgcttcta cacaccggga acagaatctg
     1381 cttacttcgg tattatcggt gcagtagcaa tcgtcggtgg tatcttgcta tttactgtga
     1441 aagaaccaat caaaaaacta atgggctcta ttcattaatc aatgaacata aaaacaggaa
     1501 ttgcttattt gcagcagttc ctgtt
//
LOCUS       DAZKKW010000218         1514 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.227, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000218 DAZKKW010000000
VERSION     DAZKKW010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1514
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.227"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>1514)
                     /locus_tag="ACG8PO_002942"
     CDS             complement(<1..>1514)
                     /locus_tag="ACG8PO_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coiled-coil domain-containing protein"
                     /protein_id="HFQ0208186.1"
                     /translation="GDKGITVTVKPYLNGLQMDTSGGTFTLKGTTPSNRYVDSVATSV
                     TSEEVTFSLDGTFMSEAGYYKHCYVEYRKDNQILTTQDIIFFSLGVSDISQGQADEYV
                     SQLEELIRKYNETFDAFMAETKGRVDSLNQQITDLTGQAKTLQDKLDALKEEISKLGN
                     LQVMYSNSINFGDYDYSGNPNLLSKLSYDLIENQNTSAGTLSKGENSFKYNKISAEVE
                     GGVELYYKRRGIANWLPSNKTLVMTVKLRAGADYSPVDGKLILIRYRYVDSGTGKIVL
                     DLPINSNSITQEWKEFSITGTTPTFSPQAYHPWIQFRAQDGILGEIEMSYDIKIEEGS
                     TATPFQPNLLAEPYNMCREYPNENIADHTVKFPIESGDHQIYQGYTEEELMIGQTYTI
                     TLKGTKPASQTFVAYNHWTARLGELKPVDGLTDVWSLTFTPTNVVASSPKLFRVYQYP
                     QSTVGACRIDWLKIEKGDTRTPNISEYKYRGTGMRDSNNPKDYVWDLAPEYVED"
BASE COUNT          410 a          311 c          261 g          532 t
ORIGIN      
        1 ttatcttcga catattctgg tgctagatcc caaacataat cttttggatt gtttgaatca
       61 cgcataccag taccacgata tttatactca ctaatattcg gggttcgtgt gtcgcctttt
      121 tcaatcttga gccagtcgat ccggcatgcg cctactgttg attgtggata ctgataaaca
      181 cgaaaaagtt taggtgaact cgccacaaca ttcgttggtg tgaatgttag agaccatacg
      241 tctgtcaacc catcaactgg ctttagttct cctaaacgag cagtccaatg attatacgct
      301 acaaaggttt gacttgcggg ttttgttcct ttaagcgtga tagtatacgt ttgacctatc
      361 ataagctctt cttctgtgta accttgatat atttggtggt cgccagattc gattgggaac
      421 ttaactgtat gatcggcaat attttcgtta ggatattcgc gacacatgtt gtaaggttct
      481 gctaataagt taggttggaa tggtgtagct gttgagcctt cttcgatttt gatgtcatag
      541 ctcatttcta tttccccaag tatcccatct tgagccctaa attgtatcca aggatggtat
      601 gcttgtgggc taaatgttgg agtagttcca gtaatactaa actctttcca ttcctgagtt
      661 atcgaattac tgttaatagg taagtctaaa acaatcttgc cagttccact gtcaacatac
      721 ctatatctaa tcagtataag ttttccgtca actggactat agtccgctcc agctctaagc
      781 ttaacagtca ttacaagcgt tttattagat ggtagccagt tagctatacc tcttcgttta
      841 taatatagct ctactccacc ttccacttca gctgatatct tattatattt aaacgagttt
      901 tcacctttag aaagtgttcc agctgaagta ttttgatttt caattaagtc gtatgatagc
      961 ttacttaaca aattaggatt tccactataa tcatagtcac caaagttgat gctgttactg
     1021 tacatcactt gtaagttacc taacttagaa atttcttctt tcagagcatc taacttgtct
     1081 tgtagcgttt tagcttgacc agttaaatca gtaatctgtt gatttaagct atccactcta
     1141 cctttagttt cagccataaa agcatcaaaa gtttcattat actttcgaat caactcttct
     1201 aattgcgaaa catattcatc ggcttgacct tgcgaaatgt cagacactcc tagtgagaaa
     1261 aaaatgatat cttgcgttgt tagaatttga ttgtcttttc tatattctac gtagcagtgt
     1321 ttataatatc ctgcttcact cataaatgtg ccatcaagag aaaacgtgac ttcttcacta
     1381 gttacgctag ttgcaacact atctacgtaa cggttagatg gtgttgttcc ttttaaagta
     1441 aatgttccgc cactcgtatc catctgcaag ccatttaaat atggtttaac cgtcaccgta
     1501 atccctttat cacc
//
LOCUS       DAZKKW010000219         1502 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.228, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000219 DAZKKW010000000
VERSION     DAZKKW010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1502
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.228"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>1502)
                     /locus_tag="ACG8PO_002943"
     CDS             complement(<1..>1502)
                     /locus_tag="ACG8PO_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coiled-coil domain-containing protein"
                     /protein_id="HFQ0208187.1"
                     /translation="GDKGITVTVKPFLNGLQMDTSGGTFTLKGTTPSNRYVDNVATSV
                     TSEEVTFSLDGTFMSEAGYYKHCYVEYRKDNQILTTQDIIFFSLGVSDISQGQADEYV
                     SQLEELIRKYNETFDAFMAEIKGRVDSLNQQITDLTGQAKTLQDKLDALKEEISKLGN
                     LQVMYSNSIDFGGYDYSGKPNLMSKLKSSDFNVGYHGSLTLDNEKLHFTSDGTGSIIM
                     FTRINTPQLTSGKTYTLSAKVRFDEGTTGAIDKLRLVYRTSPGEKILLEANSTNITTD
                     DVGKEITIKGTANVNYQITNLDRFYMSISFVDRDKINGGFKLYDIKIEEGSTATPYQP
                     NLLDAPYYLSKVALGENLIKPESQQPVTNSNYLINTYNIKPMVKGKKYTITLEGTKPA
                     TQVFRPFFTRATGDAWEVGDLQPVEGLTNVWSKTFTAADDSHPTTPQVQIYQVPSTSV
                     GQCTIKWLKLEEGNTRTPNISEYKYRGTGMRDSNNPKDYVWDLAPEYVED"
BASE COUNT          406 a          307 c          250 g          539 t
ORIGIN      
        1 ttatcttcga catattctgg tgctagatcc caaacataat cttttggatt gtttgaatca
       61 cgcataccag taccacgata tttatactca ctaatattcg gggttcgagt attgccttcc
      121 tctagtttta accacttaat tgtacattgt cctacacttg tgcttggtac ttgataaatc
      181 tgcacttgtg gggtagtagg gtgtgagtca tctgctgctg taaatgtttt agaccacaca
      241 ttagttaagc cttctactgg ttgtaagtca ccaacctccc atgcatctcc tgttgctcgt
      301 gtgaaaaatg gtctaaaaac ctgtgttgct ggcttagttc cttcaagtgt gatggtatac
      361 ttcttacctt ttaccattgg tttaatgtta taggtgttaa taagatagtt actattagta
      421 actggttgtt gtgattctgg tttaattaga ttctcaccca aagccacctt actcaaataa
      481 tacggtgcat caagtagatt aggctggtac ggtgtggctg ttgagccttc ttcgattttg
      541 atgtcgtaca acttaaatcc gccatttatt ttatccctgt caacaaagct aatactcata
      601 taaaatcgat ctaaatttgt gatttgataa ttaacgttag ctgtaccttt gattgttatt
      661 tctttcccta catcatctgt tgtaatattt gtactatttg cttccaataa tatcttttct
      721 cctggtgatg tacgatacac caaacgtaat ttatcaatag ctcctgtagt tccttcatca
      781 aatcgaactt tcgcactcag agtataggtt ttcccactag taagctgagg tgtattaata
      841 cgcgtaaaca taataatgct tcctgtacca tcagaagtaa aatgtaactt ttcgttatcc
      901 aaagttagtg acccgtggta accaacgtta aaatcgctcg attttagttt ggacattaaa
      961 ttcggcttac cgctataatc atatcccccg aagtcgatgc tgttactgta catcacttgt
     1021 aagttaccta acttagaaat ttcttctttc agagcatcta acttgtcttg tagcgtttta
     1081 gcttgaccag ttaaatcagt aatctgttga tttaagctat ccactctacc tttgatttca
     1141 gccataaaag catcaaaagt ttcgttgtac tttcgaatca actcttctaa ttgcgaaaca
     1201 tattcatcgg cttggccttg cgaaatgtca gacactccta gtgagaaaaa aatgatatct
     1261 tgcgttgtta aaatttgatt gtcttttcta tattctacgt agcagtgttt ataatatcct
     1321 gcttcactca taaatgtgcc atcaagagaa aacgtgactt cttcactagt tacactagtt
     1381 gcaacattat ctacgtaacg gttagatggt gttgttcctt ttaaagtaaa tgttccgcca
     1441 ctcgtatcca tctgcaagcc atttagaaac ggtttaaccg tcaccgtaat ccctttatca
     1501 cc
//
LOCUS       DAZKKW010000220         1495 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.229, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000220 DAZKKW010000000
VERSION     DAZKKW010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1495
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.229"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..389)
                     /locus_tag="ACG8PO_002944"
     CDS             complement(<1..389)
                     /locus_tag="ACG8PO_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFQ0208188.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHM"
     gene            complement(542..1453)
                     /gene="vanY"
                     /locus_tag="ACG8PO_002945"
     CDS             complement(542..1453)
                     /gene="vanY"
                     /locus_tag="ACG8PO_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-A/VanY-F/VanY-M family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HFQ0208189.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYELWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
BASE COUNT          480 a          284 c          201 g          530 t
ORIGIN      
        1 ttcatatgct tattgcttaa accatataat ttcagtccaa gaaagcctcc aacagtattt
       61 gtaattacat ctgttatgtc tgtcgctcca atagcgaaga taaattgaat tatttcaaaa
      121 gtaagactta aaaccagtac aaaagcaaac ttaggtaaaa atccgatttc tttaaaattg
      181 acattcaaaa gcaagccaaa tggaataaag attataacat tatctatcat ctctctgaaa
      241 ttcccagtag cagtaaatgg agtcaagtta agacttcttt gatgataatt aaatactgat
      301 aaaatattgt attgtaattt gaataacact aaccagatta gtgtcactaa atataaagct
      361 agcaatcctc tagataatat ttttcccaat ataatttcct ctaattcttt tgtttaagtt
      421 gtatcattag tatagcaatg tcctagttaa cgtgggtttt aaattaaaaa gctaacgtta
      481 ccccaaaagt gctagtacaa taatgctgtt cattaatttc gttggtttca ttccgccatc
      541 cttacctcct tgaattagta tgtgttgatc cgggaaacac tgtcacaatt acaccgtcta
      601 tattgtttcc tgatatctca taacgaagat tagtcggcac atgaatggtg gtatttttag
      661 taacaggata ataaaagatc tcatattttt ccccatttac actaacagaa atggtttttt
      721 cttcttttag gtaatccata tattcctcga gaacgaaatt cttttctttc ataatcgcac
      781 tatgtggtaa accaacatag cgaatatgcc atagttcata ttgaattcct gttaactctg
      841 ttttgtcctc tggataacgt aaaatgaacc cgtatttcca agcattttct tctatccact
      901 ttccttcagg ggctcgttcc attttcgtca agcttgatcc tacatctagt gataaacctg
      961 aattatgctc actataacct gctggtaagg catactcagc ccccatttct tggtaaagca
     1021 cactttgctc atcaaagtct cgatagccac tattaataat aaaatgacta acgccaccct
     1081 ttacagcatc attgaccatc tctgaaaatt tttgtgctat ttcttttgac atataaatat
     1141 tactatcaag caacccgtat ccatttatta attcgtcatg tttagataaa ttcacgatat
     1201 ctgacttcac actttcttgg cgaacaggat atttactatt gattaatagc agatttcctt
     1261 gataaacctg ttcttctgta attgttttct cttgggtatt ttcagaagtc ccactatttt
     1321 ctaaatgttc tttgggattt tgatcataat tttgaaattc gactttttcc tgagaaaaca
     1381 gtgcttcatt aacgtagtca taacctaagt atattaagaa taacaataac aataaaaaaa
     1441 acaacttctt cattttcagt ctcctcctta actatatagt tggaagtatg aaata
//
LOCUS       DAZKKW010000221         1475 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.230, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000221 DAZKKW010000000
VERSION     DAZKKW010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1475
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.230"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..793)
                     /locus_tag="ACG8PO_002946"
     CDS             complement(<1..793)
                     /locus_tag="ACG8PO_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0208190.1"
                     /translation="MKNLQLKFIVDPSIVRWFRIINQFERNQLATKGELAKSNHVSER
                     TIQTDVNKMKDYFSESVQFVSTKSGYSFQKERPVLFLKQKEQLLENEILFELLGNVFY
                     GELEDMTELIDRFHLSEATFRRYLKQIQPVLEEYGLELSLYPLDLKGEEANIRKFFKD
                     FYYEGEMTPHTLVPPRDLHELVQATFYDKFEYFSIGTGTSPAEFYYSLYIAIERVRQG
                     KTIAIPQGLIERVIASENFRLMYSLKEAIQTNYQVALSEEEFCWLY"
BASE COUNT          487 a          284 c          244 g          460 t
ORIGIN      
        1 aatacaacca acaaaactcc tcttctgata aagcaacttg ataatttgtt tgaatcgctt
       61 ctttcaagga atacattaag cgaaaattct cactagcaat tactcgctca atcagacctt
      121 gaggaatggc tatcgttttt ccttgtctca cacgctcaat cgcaatatac aaactataat
      181 aaaattcagc aggagaagtc cctgttccaa tcgagaaata ctcaaattta tcatagaatg
      241 tagcttgcac aagttcatga agatcccttg gtggaacaag agtatggggc gtcatctccc
      301 cttcataata gaagtcttta aaaaacttgc gaatatttgc ctcttctcct tttaaatcaa
      361 gaggataaag actcaattct aagccgtatt cttccaaaac tggttgaatc tgcttcaaat
      421 accgacgaaa agtcgcttca gataaatgaa aacgatcaat tagctccgtc atatcttcca
      481 attcgccata aaagacattc cctaacaact caaataaaat ttcgttctcc agtagttgct
      541 ccttctgctt gagaaaaaga accggtcttt ccttctgaaa agaataccca cttttcgttg
      601 aaacaaattg aacactttca gaaaagtagt ccttcatttt attgacatct gtttgaatgg
      661 ttcgttcaga tacatgatta ctctttgcta attctccttt tgtggcaagt tgattgcgct
      721 caaattgatt gataatccga aaccagcgta caatcgatgg atcaacaata aattttaact
      781 gtaaattttt catcaacaac cctcctctta gaaaaaaatt tcatcctata cctatataat
      841 tacgatacca ttttttctcc tttataggta atgttttggg gaaggaaaac aagtttctat
      901 tactcacctg ataaaatcaa taccgtcgac tatactgcgg aattgtttgg gtatagcaaa
      961 taagaccaaa tgaaaaaact tggtcttgga taaaaaatgt aaccgatttc tatttaccca
     1021 aaaagaggat atgctatact taatgagaca acttactatc attcaggtgt agtattgttc
     1081 ggtcgtggta ccccaacaac tttacaattt cttttgaaac gttggttgtg aggagggttg
     1141 ccaatgaatc acagtggaat gggaaaggtt acgttctgaa atagaatgtt gcaaaatatt
     1201 ctagcaatct tatcgatcat taacttgatc ttagagattg cgaacaaagt aaaaagctta
     1261 aaggactagt cctttaagcc acactcatta ccactgccga agcagtttcg taaaaggtga
     1321 taccagcacc ttttacctac acctgaatta tagcaagttt tttggggtta gtcaaaatag
     1381 ctaataatgg tgtagtgcaa tttctttttg aaatgttggt tgtatgctaa tgaatcccaa
     1441 tgaactgggg aaggttacgt tctgaaatag ataga
//
LOCUS       DAZKKW010000222         1436 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.231, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000222 DAZKKW010000000
VERSION     DAZKKW010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1436
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.231"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1351
                     /locus_tag="ACG8PO_002947"
     CDS             <1..1351
                     /locus_tag="ACG8PO_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional aminoglycoside N-acetyltransferase
                     AAC(6')-Ie/aminoglycoside O-phosphotransferase
                     APH(2'')-Ia"
                     /protein_id="HFQ0208191.1"
                     /translation="RDFILYLKLCNRTESLKKHYTEPWEDEVFRVIIEYNNVPIGYGQ
                     IYKMYDELYTDYHYPKTDEIVYGMDQFIGEPNYWSKGIGTRYIKLIFEFLKKERNANA
                     VILDPHKNNPRAIRAYQKSGFRIIEDLPEHELHEGKKEDCYLMEYRYDDNATNVKAMK
                     YLIEHYFDNFKVDSIEIIGSGYDSVAYLVNNEYIFKTKFSTNKKKGYAKEKAIYNFLN
                     TNLETNVKIPNIEYSYISDELSILGYKEIKGTFLTPEIYSTMSEEEQNLLKRDIASFL
                     RQMHGLDYTDISECTIDNKQNVLEEYILLRETIYNDLTDIEKDYIESFMERLNATTVF
                     EGKKCLCHNDFSCNHLLLDGNNRLTGIIDFGDSGIIDEYCDFIYLLEDSEEEIGTNFG
                     EDILRMYGNIDIEKAKEYQDIVEEYYPIETIVYGIKNIKQEFIENGRKEIYKRTYKD"
BASE COUNT          614 a          110 c          241 g          471 t
ORIGIN      
        1 aagggatttt attctttatt taaaactttg caacagaacc gaatcattaa aaaaacatta
       61 tacagagcct tgggaagatg aagtttttag agtaattatt gaatataaca atgttcctat
      121 tggatatgga caaatatata aaatgtatga tgagttatat actgattatc attatccaaa
      181 aactgatgag atagtctatg gtatggatca atttatagga gagccaaatt attggagtaa
      241 aggaattggt acaagatata ttaaattgat ttttgaattt ttgaaaaaag aaagaaatgc
      301 taatgcagtt attttagacc ctcataaaaa taatccaaga gcaataaggg cataccaaaa
      361 atctggtttt agaattattg aagatttgcc agaacatgaa ttacacgagg gcaaaaaaga
      421 agattgttat ttaatggaat atagatatga tgataatgcc acaaatgtta aggcaatgaa
      481 atatttaatt gagcattact ttgataattt caaagtagat agtattgaaa taatcggtag
      541 tggttatgat agtgtggcat atttagttaa taatgaatac atttttaaaa caaaatttag
      601 tactaataag aaaaaaggtt atgcaaaaga aaaagcaata tataattttt taaatacaaa
      661 tttagaaact aatgtaaaaa ttcctaatat tgaatattcg tatattagtg atgaattatc
      721 tatactaggt tataaagaaa ttaaaggaac ttttttaaca ccagaaattt attctactat
      781 gtcagaagaa gaacaaaatt tgttaaaacg agatattgcc agttttttaa gacaaatgca
      841 cggtttagat tatacagata ttagtgaatg tactattgat aataaacaaa atgtattaga
      901 agagtatata ttgttgcgtg aaactattta taatgattta actgatatag aaaaagatta
      961 tatagaaagt tttatggaaa gactaaatgc aacaacagtt tttgagggta aaaagtgttt
     1021 atgccataat gattttagtt gtaatcatct attgttagat ggcaataata gattaactgg
     1081 aataattgat tttggagatt ctggaattat agatgaatat tgtgatttta tatacttact
     1141 tgaagatagt gaagaagaaa taggaacaaa ttttggagaa gatatattaa gaatgtatgg
     1201 aaatatagat attgagaaag caaaagaata tcaagatata gttgaagaat attatcctat
     1261 tgaaactatt gtttatggaa ttaaaaatat taaacaggaa tttatcgaaa atggtagaaa
     1321 agaaatttat aaaaggactt ataaagattg attatataat atatgaaaag ctattataaa
     1381 agacattagt attaaatagt ttaaaaaaat gaaaaataat aaaggaagtg agtcaa
//
LOCUS       DAZKKW010000223         1436 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.232, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000223 DAZKKW010000000
VERSION     DAZKKW010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1436
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.232"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            101..1402
                     /locus_tag="ACG8PO_002948"
     CDS             101..1402
                     /locus_tag="ACG8PO_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFQ0208192.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
BASE COUNT          472 a          288 c          263 g          413 t
ORIGIN      
        1 gaaaatcatt ctatcggtcc cttttgagca tgaaaaaaca aaacatacag aagctccgtt
       61 agaattgaat caccacaaca caatcaaagg agcgttctgt atgcctgatc ttcatcatat
      121 caaactacta cttggaatca aagataaaaa cattttcatc acaaatgtgg aatcgaagag
      181 cattaagaat atcaagagtc ttgttgtttc tgcgacgtta agtaaagaaa ttcgtcgctg
      241 tcctttgtgt aaacagatga atcatgaagg catgatcgtt aagaacggaa agaaaaaatc
      301 gctcattcaa cttaataaat gtgcgaacca actgacttat cttgctttag cgaaacaacg
      361 ctaccactgt cgtggctgcc acacctattt tacggcgaat acctacattg tcgatagaaa
      421 ctgctttatc gctaaacaag tgcgctataa aattttagaa gagctaactg aaaaacaagc
      481 aatgacgaca attgctaagc attgcggcgt ttcttggtcg accgtttctc ggacactcgc
      541 ttctctgatc cccatgacaa aagtaaaaag aaattggtta ccgcgttgtt tattaatgga
      601 tgaatttcgt tcactgaaaa atcaagtcgg tccctactcc ttttcttgta tggatggcga
      661 tactggaaaa ttactggata ttcttccttc acggaagaaa aaggctctcg tttcttattt
      721 tatgcaattt gaacgacgcg cacgattgaa ggtaaaggtc atcgtgacgg acatgaatgt
      781 ttcttatgcc tctctgataa aggaatgttt tccaaaggca aaactcgttg tggatcgttt
      841 tcacattgtc aaacatctca ttcgtaaatt tgaggatatt cgcgtacgaa tcatgaaaag
      901 ctttgatcga aacgatccaa cacaagccaa atattatcga caattaaagg cgttgtctcg
      961 gcttctgatc aaacgacagg acaagcttgt ttatgacaaa tggaccaaat ggcgcaattt
     1021 tggttgggcg tatctcaccg aaagtgaagt ggtggatcgt ctgctttcga tctctgacga
     1081 attacgaatc gcctattttt actatcaaga aattcttcaa gcgtttcacg acaaagaggc
     1141 ggacactttc tttaaacttg taagaacgat gcccaattcc gttccaaaag aactgcattc
     1201 tataaaaaaa gcttttataa actatgaatc cggcattcgt ttagccctcg aattgcctta
     1261 ttcaaatgcc aaaatagaaa atctgcatac ccacatcaaa gcattgaaac gagtcgctta
     1321 tggctttcga agctttcgga aaatgaagac cagaattttc ttactaaata atctgattac
     1381 ctatgaatca aaaaacatct aaagtagaaa aggaattcta ccttagatgt tcttat
//
LOCUS       DAZKKW010000224         1424 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.233, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000224 DAZKKW010000000
VERSION     DAZKKW010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1424
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.233"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(150..1337)
                     /locus_tag="ACG8PO_002949"
     CDS             complement(150..1337)
                     /locus_tag="ACG8PO_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HFQ0208193.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT          414 a          309 c          249 g          452 t
ORIGIN      
        1 taaaagttca acctcatcaa aagcacaatg ttttgcgttg tgaacctaac tgccatgcct
       61 ttctttctga ggagagaggt caggagcagt agcgcagcga ctcttgatgt ctctcctcag
      121 aaagaagaga ctcgcacagg ttcatggtgc taaatgttat tcgaattaaa ttctaaatat
      181 tttcgtggag aagccagcca ctcagtgtct ttttccatca aaagtgttcc aattatccga
      241 gtgatggaat tgatatttgg aaaaatccga atcactcgtt ctctccggcg aatctcttcg
      301 ttcaaccgtt ccaacacatt ggttgtacga atccttcggc ggatattttc aggaaaagac
      361 aaaatttgaa tagcatcttc aaagccgttt tctaaaatgt cgcaagcagc acttaatttt
      421 gaatcacaac catatttttc caagaagtgc tcttttcttt cacgtgtcaa ttcaagttca
      481 gaggctttaa agatactctt caactcgtct tttacatcag aagaaacttt ctttggcagc
      541 ttatcaaaaa tatttctcaa aaagtgggct tggcatcttt gccagctcgc acctaaaaag
      601 ttcttgcgaa cagccttaac taaccctttg tgtgcatcag aaatgaccag ttttacaccg
      661 aacaaacctc gttgaacgaa cgattgaaaa acggtatccc aattatcttc cgattcgccg
      721 tcttgaacat caaaaccaag aatcgtacgt cgtccatcag aatcaatgcc gataccaata
      781 tatacacctt ttgagacaat acgatgattt tcccttactt tcatatacag ggcatcacac
      841 atgaaaaatg gatattccga gccttctagg ctccggtttc tccactcaaa aacaaatgga
      901 tccaactgtt tcatcagatt tgaaacgaat gatttagaaa cagtcgcacc atccgttagc
      961 agttcaactg ttttagttac tttgcgagtg gagacaccag aaatgaccat ttcaatcatg
     1021 gcagcgatca aagctttttc gcttcgctga tatctttcaa agatagtagg actaaactta
     1081 ccgtctctgg ttcgtggaac atgaagttct aacgtaccaa ttcgagttgt aaacgaacga
     1141 tcgtagtaac catttctttg tgattgccgt tcttctgaac gttcataggc atttgcctga
     1201 atatattgat ttcgttgttc ttccatcagc tgattaaaca tgatagtcat aagtgttttt
     1261 gcggtatcat tcgcaccgct ttcgtttata attgcttgaa tttcttcgct ttcaaaagta
     1321 aaatgtactt gagtcattga tatcagtcct attcgagtat gttttctgca aaaaacattg
     1381 tactcctaac tgagatcaat ggcttttttg atgcttcact ttta
//
LOCUS       DAZKKW010000225         1405 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.234, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000225 DAZKKW010000000
VERSION     DAZKKW010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1405
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.234"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(13..1152)
                     /locus_tag="ACG8PO_002950"
     CDS             complement(13..1152)
                     /locus_tag="ACG8PO_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HFQ0208194.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
BASE COUNT          363 a          305 c          256 g          481 t
ORIGIN      
        1 attaaaaaaa tcttacgcta agtaggataa aataattcct tgatattttt cgatcatatc
       61 caaataatta tcgatttcaa catactcatc tacttgatga ggcaaagtaa cgacaccagg
      121 cccaaagacg acaaagtcga aggaatggtt agctttcgta aattcggctg catccgttgt
      181 tgctgccgct ccaactaaag gtaatggctg gctgaattgt tgctgaatgc aatgaatcaa
      241 aggagaatct ggatcagctt tgacaggtat tttgttgtaa tcgatcatca attccaaatg
      301 atagtctgtc tcttgattca attcattgac gattgattgc agcaatgcaa tgattttatc
      361 atttgggtat tctggaatcg atcgaatgtt cccttggagc tgcgcatggc ttgggatgct
      421 gttgacttga ttcccgccac tgatcaatgt aacattatgg attgtttttc ctaggacagg
      481 attctcgatt gtttcagcga ggtgattcat ttcagcattt gctttcgtaa tgaattcatt
      541 taggtgattg attgcattat aaccttggtc tggcatagag ctatgcgctt cttttccgtg
      601 cgaggtgact gtatagttga ttgatcccat gtgcgtatac atcaaactgt aattggttgg
      661 ctctccgatg atcaaggcat ctaagtcatc tacatagcct gctttcgtga gctgttcccc
      721 acctagttca cccacttctt ctccgacagt ggctaataat ttgactgttc cgttaaatgg
      781 ttttcctgac tctttcagtt cgatcatggc aatcaccatt gctgccaagc cactcttcat
      841 atctgtagca ccacgtccgt acaaacggtt cccatggatt tctgctgcaa agggtgcata
      901 ggtccaagac gattcatcac cagccgccac gacatccata tgcccagata ggcctaacac
      961 ttttcctgat tggccctttt ggtaacgagc aatcaaatta tctctaccat ctctgtaaga
     1021 aacaatctct cctgttatgt catgctttgc caaaagctta ttcaaatagg ctgctacttc
     1081 accttcgttt ccattcactg atttaattcg gataatctct tgtaaaatcg cgattttttc
     1141 ttcttttttc atctaatcct ctcccttgcc atttcttttt attgtatttg agtgtgctcg
     1201 aattttcaaa taggagcaac caaagtaaaa atgaagaaag agagccactt tatgaaaaat
     1261 atgctgctac ttccctccta ttttatttat acgagaggac ttgcccatag attcctattc
     1321 tactgttcta tccttaaaag ctcttatatg atgctagtta ttgttagctt tcctttttca
     1381 aggatgatct tttcttgtac cattc
//
LOCUS       DAZKKW010000226         1394 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.235, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000226 DAZKKW010000000
VERSION     DAZKKW010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1394
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.235"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            468..>1394
                     /locus_tag="ACG8PO_002951"
     CDS             468..>1394
                     /locus_tag="ACG8PO_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS256 family transposase"
                     /protein_id="HFQ0208195.1"
                     /translation="MTQVHFTLKSEEIQSIIEYSVKDDVSKNILTTVFNQLMENQRTE
                     YIQAKEYERTENRQSQRNGYYERSFTTRVGTLELKVPRTRDGHFSPTVFERYQRNEKA
                     LMASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLEPMVNEWQNRLLSEKN
                     YPYLMTDVLYIKVREENRVLSKSCHIAIGITKDGDREIIGFMIQSGESEETWTTFFEY
                     LKERGLQGTELVISDAHKGLVSAIRKSFTNVSWQRCQVHFLRNIFTTIPKKNSKSFRE
                     AVKGIFKFTDINLAREAKNRLIHDYIDQPKYSK"
BASE COUNT          494 a          262 c          242 g          396 t
ORIGIN      
        1 aaaaagttca aaagatcaag tgtgcgcttc gctcccactc ccctcgacgt tttagtagcc
       61 tttccctcac ttcgttcagt ccaagccaac taaaaagttt tcgggataca ttctccttct
      121 cttcctaata atcaattaaa attttaatct ttatatttct gctaatcact caggaaacag
      181 tacagaaaaa caaacacgta tcatagatat aaatgtaatg gcatagtgcg ggttttattt
      241 tcagcctgta tcatagctaa acaaatcgag ttgtgtgtcc gtttttgggc gttctgctag
      301 cttgtttaaa gtctcttgaa tgaatgtatg ttctaagtca aaaaactctg acagcgcctt
      361 tatatagata aagtccgtat aattgtgtaa aagtaaaaag gccatataac agtcctttta
      421 cggtacaatg tttttaacga caaaaacata cccaggagga cttttacatg acccaagtac
      481 attttacact gaaaagcgaa gagattcaaa gcattattga atattctgta aaggatgacg
      541 tttctaaaaa tattttaaca acggtattta atcaactaat ggaaaatcaa cgaacagaat
      601 atattcaagc aaaagaatat gaacgaacag aaaaccgaca aagtcaacga aatggctatt
      661 atgagcgcag ctttacgaca cgtgtaggca cgctagaatt aaaagtaccc agaacacgtg
      721 atggccattt ttcacccaca gtgtttgaac gttatcaacg aaacgaaaaa gccctcatgg
      781 cttcaatgtt ggaaatgtat gtatcaggcg tttcaactcg taaagtatca aaaattgtgg
      841 aagaactttg tggtaaatcc gtctctaagt ccttcgtttc tagcttaaca gaacagctag
      901 aacctatggt taacgagtgg cagaatcgtt tattatcaga aaaaaattat ccttacttaa
      961 tgaccgatgt actctatata aaagtacgag aagaaaatcg agtactctca aaaagctgtc
     1021 atatagcgat tggaataacc aaagatggcg accgtgaaat tatcggcttc atgattcaaa
     1081 gtggcgaaag cgaagagacc tggacaacat tttttgaata cctaaaagaa cgcggtttac
     1141 aaggtacgga actcgttatt tctgatgcgc acaaaggatt agtctctgcc attagaaaat
     1201 ccttcaccaa cgtaagttgg caaagatgcc aagttcactt cctaagaaat atctttacca
     1261 ccattcctaa aaaaaattca aaatctttca gagaagctgt taaaggaatt tttaagttca
     1321 cagatattaa cttagcgcgt gaggctaaaa atcgattgat tcatgattat atcgatcaac
     1381 caaaatattc aaaa
//
LOCUS       DAZKKW010000227         1386 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.237, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000227 DAZKKW010000000
VERSION     DAZKKW010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1386
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.237"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..245
                     /locus_tag="ACG8PO_002952"
     CDS             <1..245
                     /locus_tag="ACG8PO_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0208196.1"
                     /translation="TVTENYLIVSPLLALAASKTIRVTGTMIFDKVGWPSRPPQSRNW
                     KEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            255..458
                     /locus_tag="ACG8PO_002953"
     CDS             255..458
                     /locus_tag="ACG8PO_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0208197.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            669..1253
                     /locus_tag="ACG8PO_002954"
     CDS             669..1253
                     /locus_tag="ACG8PO_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0208198.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
BASE COUNT          487 a          195 c          260 g          444 t
ORIGIN      
        1 taactgttac tgaaaattat ttaatagttt ctccattact tgctctagca gcttctaaaa
       61 cgatacgagt gacaggaacg atgatttttg ataaagttgg ttggccttct agacctcctc
      121 aatcacggaa ttggaaagaa aaaagaaatg gttattggta ctatggaaaa ttgtatttac
      181 aaagttataa atcagctgga aacaattgga ttgcgactta ttcaggaacc ttaactagaa
      241 agtagggatt ttttatgcca gaatttgtga acacaccaaa tttttatcaa gatagcgata
      301 ttctaagtaa aattaattta ccgaaagaag aatcagcttt acaagttcag cgaactttac
      361 aggagacaca aaaaattcaa aaatcaattt ctaatctaga agattctgaa gaaaaaagaa
      421 aaaaaagaga ccaaaaaaca tatcaaaaag aaaaataatg attttaaaaa aagagtgtct
      481 agtcgacatt ctttttttct aattgaaagt gttcataaaa tcacccattt ctggacactt
      541 tctttttttg ctttattttt caaaaaaaca attcatttca tgttcataaa cccataacaa
      601 tatctatgtt cagttaaact atatggataa agttatgtta caataaaatt aatgaaggag
      661 tgattttttt gaaaatagga tacgcccggg tatctactgg tttacaaaat ttggatttac
      721 aagaagacag cctaaataaa tttggttgtg aaaaaatttt tactgatcat atgagtgggg
      781 caaaaagtaa tcgaccagga ttagaaatgg ccattgattt tgttcgctct ggcgatacac
      841 ttgtcgtttg gcgcttagat cgtttaggta gaaatatgga agatttgatt tcaatcgtca
      901 atcgactgaa tgagcgtggt gtgagttttc atagtattca agaaaatatt acgatggaca
      961 aatcaagttc tacaggacag ctcatgtttc atttgtttgc ggcatttgca gaatttgagc
     1021 gtaatttgat cctggaacga tccgcagcag gacgagcagc tgctcgtgca agaggacggt
     1081 ttggtggtcg tcctgaaaaa ttgtctgaac aagatttgga attactgaag actttagtgg
     1141 atagcggtac acctattaag actattgcgg aacggtggaa cgtctcaaga accaccattt
     1201 atcgttattt ggcaaaaatg gaggagaaaa atggaaagac aaattctcat tagtttttgt
     1261 catttaatgc tgaaaatttt tgccatttat ctagaaaagt gacaatagta tgagaatttt
     1321 ttgtaagtgt ctactgataa cttattctca aatgttgtaa ggattttatt catttgttaa
     1381 tattaa
//
LOCUS       DAZKKW010000228         1368 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.238, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000228 DAZKKW010000000
VERSION     DAZKKW010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1368
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.238"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            82..>1368
                     /locus_tag="ACG8PO_002955"
     CDS             82..>1368
                     /locus_tag="ACG8PO_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa5 family transposase"
                     /protein_id="HFQ0208199.1"
                     /translation="MNDSIKKMLRLIEKDLMITEVSYETFQKKKTLIVDAVFSPAPHT
                     CRNCGSTVVDGNGKVIVVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKNCQVSLTTVIRTLKEFKSYLPKQSK
                     RILPRVLMVDEFRSHASIEDKMSFICADGETGQLIDVLPTRKLPRLTSYFLGCANPEE
                     VKFLVTDMNAAYFQLTKRILPNAKVVIDRFHIIKHMNQAFNELRIREMNELRKVGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLGFSTSLKEAY
                     PYFHELVEAFRDKDPDLFFSLLAELPETLDDGFREKLQNLLTYEEGITNAMIYPYTNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRIFLINQLIK"
BASE COUNT          466 a          256 c          259 g          387 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcacgac aaaataaaca
       61 actgagagga cgatatgcta tatgaatgat tctatcaaaa aaatgctgcg attaatagag
      121 aaagatttga tgattacaga ggtctcttac gagacctttc agaagaaaaa gacgttgatc
      181 gtcgatgctg ttttctcacc tgctccccat acctgtagaa attgtggttc tactgtggta
      241 gatggaaacg ggaaagtaat tgtagtgaaa aatgggaaga aggaaacgat tgtccgcttt
      301 gaacaataca atcacatgcc tttggttatg cgcttaaaaa agcagcgcta tacctgtaaa
      361 aactgtcgaa cccattggac ggctcaaagt tattttgttc aacccagaca ttcaatcgcg
      421 aatcatgtca gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaaatt gtcaggtatc tttgaccacc gttattcgta cgttgaaaga gtttaaaagc
      541 tacttaccaa agcaatctaa gaggattctc cccagagtat tgatggttga tgaatttcgt
      601 tcgcacgctt ccatagaaga taaaatgagc tttatttgcg cagatggcga aacaggacaa
      661 ttaattgatg tcttgcctac gcgtaaattg cctcgattaa caagctattt cttaggctgc
      721 gctaatcctg aagaagtaaa atttttagtc acagacatga acgccgccta cttccagctt
      781 accaaacgta ttctgccaaa tgcgaaagta gtgattgatc ggtttcatat tatcaaacac
      841 atgaatcaag cctttaatga gttgcgtatc cgtgaaatga atgaacttcg taaagtagga
      901 cagaaaagtc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaata caaaacatgg aaaagttttc gagcaccaaa atacccattt
     1021 cttacggaag cgatgatgat tgatcgattg cttggctttt ctacgtccct gaaggaggcg
     1081 tatccctatt ttcatgaatt agttgaagcc tttcgagaca aagaccctga cttatttttc
     1141 tccttattgg cagaacttcc cgaaacgtta gatgacggct ttcgggaaaa gctccaaaac
     1201 cttctgacct atgaagaagg catcaccaac gcgatgatct atccttacac caatggaaaa
     1261 atagaagcga agaataccca tataaagaca atgaaacgag tatcctacgg atttaaatca
     1321 tttgagaaca tgagaattag aatctttttg atcaatcaat taatcaaa
//
LOCUS       DAZKKW010000229         1359 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.239, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000229 DAZKKW010000000
VERSION     DAZKKW010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1359
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.239"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..484
                     /locus_tag="ACG8PO_002956"
     CDS             <1..484
                     /locus_tag="ACG8PO_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFQ0208200.1"
                     /translation="KEAIDQIKTAVLYPHNGLPILLMGVSGSGKTFLAEKIYEYAKEQ
                     QVIESNAPFIAYNCAQYFNFSINKIVENLTILDAMKVLRLVQKAVKELEKLMGIEFSN
                     NQLFLLYMHCCSMIERILRKESVDEQADIKEYIQKEGHNYEISKYPAYKTKFESTVIK
                     "
     gene            complement(691..1215)
                     /locus_tag="ACG8PO_002957"
                     /pseudo
     CDS             complement(691..1215)
                     /locus_tag="ACG8PO_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          445 a          254 c          260 g          400 t
ORIGIN      
        1 aaaggaagcg atcgatcaaa tcaagacggc tgtcttgtat cctcataacg gattaccgat
       61 tttattgatg ggtgtaagcg gttcaggaaa aacctttttg gcagaaaaaa tttatgaata
      121 cgccaaggaa caacaggtga tagaatcaaa tgctccattt atcgcctata attgtgctca
      181 atactttaat ttctcgatta acaagatcgt agaaaattta acgatccttg atgcaatgaa
      241 ggttttgaga ttggtacaaa aggccgtcaa agaactcgaa aaactaatgg ggatcgaatt
      301 ttcaaacaat caattgttct tgctttacat gcactgctgc tcaatgatcg aacgtatttt
      361 acgcaaagaa agtgtagatg aacaagcaga tatcaaagaa tatattcaaa aagagggaca
      421 taattatgaa atatcaaaat acccggctta caaaacgaag tttgaaagta ctgtaataaa
      481 gtaagattta aataagcata aacaaggtaa gaaccatagg ggctgtgtca agtccaaaat
      541 cttgtgtaaa aaggaaatct caaagtacga tgttttgcct tgttgagcct agctacggtc
      601 caatatgctg gtaagaagtg tcaagagcaa aaagcgaagc gtctcttgat acttctcacc
      661 agtatattat actattggcg gcttaatgtg ctaaatctcg ttacgatcaa attcaagata
      721 ttttctaggt gcagaagtcc attcgtcatc cttttccatc aataaagcac cgatgatacg
      781 aataacagaa tcacaatttg ggaaaattcg gacaacacgt tcacgacggc gtacttcttc
      841 atttagtctt tcaagaacat tcgttgttct caatcgttga cggatatttt ctggtaaaac
      901 cattacttgc atggcatctt cgaagccctc atccaatatt ttacaggcag ttgcgacttt
      961 cttgtcttcc ccatatttat ccataataag ttgtttacgt tctccagcca gctcgatgct
     1021 tgccgttttg aagatggctt taaagtcttc tttgatatcg cttgagattt tcttggagaa
     1081 tttgtcggaa atgttgcgaa ggagatgtgc ttggcatctc tgctagctcg cacaaagaag
     1141 gtattctttc acagcttttg tgagccctat atgcgcatcc gagatgacca tctttactcc
     1201 tgaaagaccg cgttctttca atccgagagt gtaaaatatt ttgtgtaaat agaaaaaagg
     1261 aagtcccttc agtagaatag agttaccaca acacattcac agaaaagagg acttccatat
     1321 gaacgatttt actacagaaa ttctaaagac tctagcgaa
//
LOCUS       DAZKKW010000230         1349 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.240, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000230 DAZKKW010000000
VERSION     DAZKKW010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1349
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.240"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..285
                     /locus_tag="ACG8PO_002958"
     CDS             <1..285
                     /locus_tag="ACG8PO_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection protein"
                     /protein_id="HFQ0208201.1"
                     /translation="TKEQQLQFHFVKKEVYELGKVEGTSFQGNPITMYDKERTLCDIW
                     NPRYEFEYEMKLEALKEYMEDPLRDPSKLRDYMTKLHVEKEMKYHMQSLY"
     gene            298..1197
                     /locus_tag="ACG8PO_002959"
     CDS             298..1197
                     /locus_tag="ACG8PO_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HFQ0208202.1"
                     /translation="MTPTQFAAKTRNLAKSTGLNAQLVQRHFMMDRLIEQISESKYKN
                     DFILKGGFLLGSKYGIDRRTTIDIDTTFRNSKLTEEKLKSIFNDLTRKPTKEGIVFSI
                     QGMKETREADFYPGFQIKLVANLEKVRVPFKMDVTTGDSIYPEVDTHYHQLMFEDKKV
                     EIPAYPTEQIIAEKLSATFSFGTDNSRAKDYYDLFTIPKLENIDTKELYKSVRNTFTK
                     RGDTKPLSFYYEREMDKIIGNDYLEKQWEKYRSVNTFANSISFTDTVNEIDHLMKSVI
                     KIENQERKRQFKRSQQMNQEMER"
     gene            1200..>1349
                     /gene="ssb"
                     /locus_tag="ACG8PO_002960"
     CDS             1200..>1349
                     /gene="ssb"
                     /locus_tag="ACG8PO_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003219228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFQ0208203.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGNR
                     EADFIN"
BASE COUNT          548 a          196 c          255 g          350 t
ORIGIN      
        1 acaaaagagc agcagttaca atttcatttc gtgaagaaag aagtttatga actaggaaaa
       61 gtagagggaa cttcctttca aggaaatcct attactatgt atgataaaga gcgtacactt
      121 tgtgatattt ggaatccgag atatgaattt gaatatgaaa tgaaattaga agcattaaaa
      181 gagtacatgg aagacccatt gagagatcca agtaaattaa gagattacat gacaaaacta
      241 catgtagaaa aagaaatgaa ataccacatg caaagcttat attgatggag gaaaaagatg
      301 acaccgacac aatttgcagc aaaaacaaga aatttagcaa agagtacagg attaaatgca
      361 cagttagtgc aacggcattt tatgatggat cgtctaattg aacaaatttc tgaatcaaag
      421 tacaaaaatg acttcatttt gaaaggcggc tttttattag gatcaaaata tggaatcgat
      481 agaaggacaa caattgatat cgatacgaca tttagaaata gcaaattaac tgaagaaaaa
      541 ttgaagagca tttttaatga tttaactaga aaacccacaa aagaaggcat tgttttctct
      601 attcaaggaa tgaaggaaac aagagaagcc gatttttatc ctggatttca aattaaattg
      661 gtggctaatt tagagaaagt acgtgtaccg tttaaaatgg acgtgacaac aggagattct
      721 atttatcctg aagtagatac acactatcac caattaatgt ttgaagataa aaaagtggag
      781 attccagctt atccaacaga acaaattatt gcagaaaagc taagtgctac gttcagcttt
      841 ggcacagata attcgagagc aaaagattac tacgacttat ttaccattcc gaaactagaa
      901 aatatcgata cgaaagagtt atacaaaagt gttcgaaata catttacaaa acgtggggat
      961 acaaaaccgt tgtcctttta ttatgaaaga gaaatggata agatcatagg aaatgactat
     1021 ttggaaaagc agtgggaaaa atatcgctca gttaacacgt ttgcgaattc catttcattt
     1081 acagatacag tcaacgaaat tgatcaccta atgaaaagtg tgatcaaaat tgaaaatcaa
     1141 gaaagaaaac ggcagtttaa aagatcacaa caaatgaatc aagaaatgga gagataaaga
     1201 tgatcaataa tgtaacttta gtaggacgct taaccaaaga tccagattta cgttataccg
     1261 cgagtggtac tgcagtagcg acttttaccc tagctgtcaa ccgaaatttc acgaatcaaa
     1321 acggcaatcg agaagctgat tttattaac
//
LOCUS       DAZKKW010000231         1341 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.241, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000231 DAZKKW010000000
VERSION     DAZKKW010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1341
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.241"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            50..>1341
                     /locus_tag="ACG8PO_002961"
     CDS             50..>1341
                     /locus_tag="ACG8PO_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-directed DNA polymerase"
                     /protein_id="HFQ0208204.1"
                     /translation="MYKERDDLNYTLIINKDGNYSWRPLVLLHPILYVDLVNCLTKED
                     HWEELKKRFSEFKKDKNINCYSIPVEASKGSKRTDLGETILNWWEYFEQKSLAQNIKY
                     QYCMFTDIADCYPSIYTHSITWAMYGKEKVKNDENKCSGSLGDKIDKKISGMQHNQTN
                     GIPQGSVLMDFVAEIVLGYVDLRLSKELKKLENLKDYKVLRYRDDYRIFSNNRNDLEQ
                     IMKVLSEVLYDLNFKLNSSKTKLSDDIIIDSIKKDKLYWEEIKASIRMNATNLDGDRE
                     IRYSINLQKHLLEIKKLADKHPNSGQLKKALSEFNKERIIKITSKPNDLLPLIGILVD
                     IMINNPGSLPQCVLIIGQLLNFENESKAREIVEMIINKYALKPNTEFMMIWFQRLTTI
                     FGEMFDSVLSSEFVSKIKDPVSNQMFSVDWLNEDAKKS"
BASE COUNT          524 a          172 c          228 g          417 t
ORIGIN      
        1 ttcaaaaaaa aaccaaccga aagatataaa agatattgta gatacacgaa tgtataaaga
       61 acgtgatgac ttaaactata cgcttattat caataaagat ggaaactatt catggcgtcc
      121 tctcgttcta ctacatccta ttctatatgt agatttggtt aattgtctca ctaaagaaga
      181 ccattgggag gagttaaaaa agaggtttag tgaatttaaa aaagataaaa atattaactg
      241 ttattctata cctgttgaag cttccaaagg ttcaaaaagg acagatcttg gtgaaacaat
      301 actaaactgg tgggaatatt ttgaacaaaa aagcttagct cagaatatta aataccaata
      361 ttgcatgttt actgacatcg ctgattgtta tccatcaatt tatactcact ctataacatg
      421 ggccatgtat ggtaaagaaa aagttaaaaa tgatgaaaat aagtgttcgg gctcacttgg
      481 cgataaaata gataaaaaaa tatctggaat gcaacacaat caaacaaatg gtatccctca
      541 gggttccgtt cttatggatt ttgtagcaga gatcgttcta gggtatgttg atttgagatt
      601 atcgaaagaa ttaaaaaaat tagaaaattt aaaagattac aaagtactca gatatagaga
      661 tgattataga atattttcaa ataatcgtaa tgatttggaa caaattatga aagtgttatc
      721 agaagtctta tatgatttaa attttaagtt aaattctagt aaaacaaaat tatcagatga
      781 tattatcata gattctatca aaaaagataa gctctattgg gaagaaatta aggcaagtat
      841 aagaatgaat gccacaaact tagatggtga tagagaaatt cgttatagta taaatttaca
      901 aaaacattta ttggaaataa aaaagttagc agataagcat cctaattcag gccaattgaa
      961 aaaagcactt tctgagttta ataaagaacg cattattaaa atcacttcaa agccgaacga
     1021 tcttttacca cttattggta tcctggtaga tatcatgata aataacccgg gttctttgcc
     1081 gcagtgtgtc ttaattatag ggcagttatt aaatttcgaa aatgagagta aggctagaga
     1141 aatagttgaa atgattataa acaaatatgc tttaaagcca aatactgaat ttatgatgat
     1201 atggtttcaa agattgacca ccatttttgg agaaatgttt gatagtgttt tatcatccga
     1261 atttgtatct aaaatcaaag atcctgtctc taatcagatg ttttctgtag actggctaaa
     1321 tgaagatgct aagaaatctt t
//
LOCUS       DAZKKW010000232         1299 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.242, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000232 DAZKKW010000000
VERSION     DAZKKW010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1299
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.242"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(62..286)
                     /locus_tag="ACG8PO_002962"
                     /pseudo
     CDS             complement(62..286)
                     /locus_tag="ACG8PO_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877485.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(840..926)
                     /locus_tag="ACG8PO_002963"
                     /pseudo
     CDS             complement(840..926)
                     /locus_tag="ACG8PO_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
BASE COUNT          461 a          217 c          177 g          444 t
ORIGIN      
        1 taggtatttt tattatatat agatttaaca tatgtcggtc ggttgattga tatagctaaa
       61 gttataactc aaccgaagcc gattcatcta cacgaataaa gttgcgagtg ttctcggcta
      121 attcataaaa attgttatgt gttccatata gcaatactgg aataacttta ttttcttgaa
      181 tctcataagc aactctatac acggtgaccg catacttaaa tccattagac caattacctg
      241 ataaacctcc agttttttgc tcttacttaa atggatcaat ggcaatttca tcacaaacct
      301 atagaggatg agtgacttct gcctaattac gttaaaaaat attgaaagta actctatgta
      361 gcaaaaaata tctagaattt catttcctca aaactgatcg atattgtatc taaaaatata
      421 tactacctaa aataggtagt atatattaaa atatgtgctt ttgttagaaa aatcaataca
      481 taaattatca tatctgtata tgtcatttag gtatttcagt tatgttataa tagttttgct
      541 gtctgctacc ccggcctaat ctggcaaggg gaggaggaga tataatgaaa tacctttttg
      601 attttgtttt tgcaatcaag ccacttgcag atgtagtgtg cagttacctc tgcaaatggc
      661 ttgattgcaa aattgccaaa aaagacagta agcctacccg ttaattctat aatatagaaa
      721 cgtaaaaaaa accccatcag cctgcacgct gatggggttt ggtgatataa tgaaatacct
      781 ctttgttact cataattata ccataattag tcacgaatgc aaccaataga aaaaactact
      841 tagaacggta gatcatcgct aataacaatg cattgtccac caaatggatt ttgtgaagca
      901 ttaaatacat tgttattaat tgtcgctgct tttaactagg cctctcccct tctattataa
      961 aagaagatat atatcatttc ttaattacct actaccttaa accaaaattt aagtactacc
     1021 taatttaagt agtacttaaa caatctaaaa aatagttatc ccaattgtta tgcttttata
     1081 atacgattat ttgtaacaaa atgaatatta tataattaaa cactaccata tttaggtagt
     1141 gtttaattat tagcaaaaca aaaaatattt attctattct cttaacttca actaagttat
     1201 gctgcacacc tttatataaa tcatatgcaa ctcattagct agacaaacta caagcatgta
     1261 gttttatctg ttaattatgt actacctaaa taaagtagt
//
LOCUS       DAZKKW010000233         1288 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.243, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000233 DAZKKW010000000
VERSION     DAZKKW010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1288
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.243"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            2..>1288
                     /locus_tag="ACG8PO_002964"
     CDS             2..>1288
                     /locus_tag="ACG8PO_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HFQ0208205.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRIFLINQLIN"
BASE COUNT          433 a          250 c          246 g          359 t
ORIGIN      
        1 tatgaatgat tctatcaaaa aaatgctgag aataacagag aaagatttga tgattacaga
       61 ggtctcttac gagacccttc agaagaaaaa gacgttggtc gtcgatgctg ttctctcgcc
      121 tactcctcgt gcttgtagaa gttgtggttc tactgtggta gatggaaacg ggaaagcaat
      181 tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt gaacaataca atcatatgcc
      241 tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa aactgtcgca cccattggac
      301 ggctcaaagt tattttgtcc aacccagaca ttcaatcgca aatcatgtta gatataaaat
      361 tgcttcttta ctgactgaaa aagtatcttt atcttttatt gcgaaaagct gtcaggtatc
      421 tttgaccacc gttattcgta cattgaaaga gtttaaaagc tatttaccaa agcaatctaa
      481 gaagatcctc cccagagtat tgatggttga cgaatttcgt tcgcatgctt ccatagaaga
      541 taagatgagc tttatttgcg cggatggcga aacaggaaaa ttaatagatg ttttgcctac
      601 gcgtaaatta cctcgattaa caagctattt cttaggttgt accaatccag aagaagtaga
      661 attcttggtg acagacatga acgccgccta cttccagctc accaaacgtg ttctgccaaa
      721 tgcgaaagtc gtgattgatc ggtttcatat tgtcaaacac atgaatcaag cgttcaatga
      781 gttgcgtatc cgtgaaatga atgaacttcg taaagccgga cagaaaagcc aggcagaaaa
      841 actgaaaaag aactggcgct ttttgctaaa aaatcgtgca aacatcaacc attatgaata
      901 caaaacatgg aaaagtttcc gagcaccaaa atacccattt cttactgaag caatgatgat
      961 tgatcgattg cttgagtttt ctccgcccct aaaggaggcg tatccctttt ttcatgaatt
     1021 agttgaagcc tttcgagaca aagaccctga cttatttttc tccttattgg cagaacttcc
     1081 cgaaacgtta gatgacagct ttcgggaaaa gcttcaaaac cttctgacct atgaagaagg
     1141 catcaccaac gcaatgatct atccttattc caatggaaaa atagaagcga agaataccca
     1201 cataaagaca atgaaacgag tatcctacgg atttaaatca tttgagaaca tgagaattag
     1261 aatctttttg atcaatcaat taatcaat
//
LOCUS       DAZKKW010000234         1288 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.244, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000234 DAZKKW010000000
VERSION     DAZKKW010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1288
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.244"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            40..858
                     /locus_tag="ACG8PO_002965"
     CDS             40..858
                     /locus_tag="ACG8PO_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFQ0208206.1"
                     /translation="MDTFQGTTFTVGNFKGGVGKTKIVSMLAYDNAMIRNRKTLIIDL
                     DPQANLTQVIARTFGITDIKTTITNGVANGELASSIIKVNENLDLLACDTSFRSFSAF
                     ANKHENELDQVTVLKKLLLPIKNNYQEIFIDVPPTISEFSDNAMAASDYSIISFQTAE
                     ESLEGVNKYVNYQKFMVDRYDLDLQIIDIVPCMVEPNDDFDEEILEEAIEKYGTVVSK
                     NLIHYQKRLRRYSKIGIHLRKYKNGNFDQWDYKAHQVFINILAELDARQEFLNK"
     gene            877..1284
                     /locus_tag="ACG8PO_002966"
     CDS             877..1284
                     /locus_tag="ACG8PO_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0208207.1"
                     /translation="MPKDFDIPKMMENGEFEPLLPNNKRQDVKISGEYNFSNSALSNT
                     RNITLKKRGRPVSIPDKRNKVSVPKKISPALNSKLSILQDYMTELQSEKGRISFEKIV
                     NTLADSYIQHRLGVAKEEHIREEIQEEFEKLPK"
BASE COUNT          520 a          183 c          219 g          366 t
ORIGIN      
        1 ttatacttta attatcaatt aaagattggg aggatttaca tggatacttt tcaagggact
       61 acatttacag ttggcaattt taaaggagga gtaggtaaaa ctaagatagt cagcatgctt
      121 gcttatgaca atgcaatgat aagaaataga aaaacgctta ttattgattt agaccctcaa
      181 gcgaacttaa cacaagtcat agcaagaact ttcggtataa ccgatatcaa aaccactata
      241 acaaatggtg tcgcaaatgg agaattagca agtagtatta ttaaggtaaa tgaaaatctt
      301 gaccttctgg catgtgacac tagttttcga tcattctccg cttttgctaa taaacatgaa
      361 aatgaattag accaagttac tgtcttgaaa aaattacttc ttccaataaa gaacaattat
      421 caagaaatat ttattgatgt accaccaacc attagcgaat tttccgataa tgctatggca
      481 gcttcagact attcgataat ttcatttcaa acagctgaag aatccttaga gggagtcaac
      541 aaatatgtaa attatcaaaa attcatggtc gatagatacg atcttgattt gcaaattata
      601 gatattgtac cttgtatggt agaaccaaat gatgattttg atgaagaaat cctagaagaa
      661 gcaattgaaa aatacggaac cgtggtatcc aaaaatctca ttcattatca aaaaagattg
      721 agaaggtatt ctaaaattgg tatacattta agaaaataca aaaatggaaa ttttgatcaa
      781 tgggattata aagcacacca agtatttata aatattttgg cagaactaga tgctagacaa
      841 gagtttttaa ataaataata aggagttgca taagtaatgc ctaaagattt tgatattcct
      901 aaaatgatgg agaatggtga atttgagcca ttattaccca ataacaaacg ccaagatgtg
      961 aaaataagcg gtgaatataa ctttagcaat agtgcattga gtaacacaag aaatattaca
     1021 ttgaaaaaaa gaggccgacc agtttcaatt cctgataaaa gaaacaaggt atcagttcct
     1081 aaaaagatct ctccggcatt aaatagcaaa ctctcaattt tacaagacta tatgacggag
     1141 ttacaaagtg aaaaagggag aatttctttc gaaaaaattg ttaatacatt agcagactca
     1201 tatatccagc atagactagg agtagcaaaa gaagaacaca taagagaaga aatacaagaa
     1261 gagtttgaaa aattaccaaa ataatttt
//
LOCUS       DAZKKW010000235         1283 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.245, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000235 DAZKKW010000000
VERSION     DAZKKW010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1283
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.245"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..450)
                     /locus_tag="ACG8PO_002967"
     CDS             complement(<1..450)
                     /locus_tag="ACG8PO_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFQ0208208.1"
                     /translation="MARKKRNPDAEKLAESILNAYQPESVDDMQDALKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKEHDNRRNGYGTKTLKTSFGEVAIDVPRDREASFEPELIP
                     KRKRDVSDIEGKVLSMYARGMSQRDIAATVEAIYGFDISHEMISDITD"
     gene            complement(724..984)
                     /locus_tag="ACG8PO_002968"
     CDS             complement(724..984)
                     /locus_tag="ACG8PO_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0208209.1"
                     /translation="MKKIDLINMIGMLIGILVNIVIFTDWLGVLFSNLIPILIIGICG
                     IILSILELFESRNTMNRIFACIILIVNLLPMVYFTFLYFALG"
BASE COUNT          454 a          227 c          183 g          419 t
ORIGIN      
        1 gtcagtgata tctgaaatca tttcatggga aatatcaaag ccataaatag cttcgacggt
       61 tgcggcaata tctcgttgac tcattcctcg tgcatacatg gaaagaacct tcccttcgat
      121 gtcggagaca tctcgttttc tcttaggaat taactctggt tcaaaggaag cttcccggtc
      181 tctaggaaca tcaatagcta cttcaccaaa actggtttta agcgttttag ttccatagcc
      241 atttcgacgg ttatcgtgtt ccttaggctc tttagaatgg gcatcataac ctaaatgatt
      301 attcaattct ccttgaagca ttttttcaaa aaggggccca aacacatctt tcaaagcatc
      361 ttgcatgtca tcgacagatt caggttgata ggcattcaga atggattcag ctaacttttc
      421 ggcatcagga tttcttttct ttctagccat cgtgtaatca cctcttgatc ttattgtaga
      481 aaaagaaaaa ccgcgaagca accacctgcc taggattaat ggttacttac acggtttata
      541 ttacactctc atcattaaaa aagtatcttt cgatattact aaacaatgaa attatctatt
      601 tgaaatttaa atctgataat atattgagtg ttccaatgag ggctttgatc ctattatcca
      661 tatctttttc agttttaata ttgatacaca ttataagaaa aagaaacttt tggttaattt
      721 atttcatccc agtgcaaaat ataaaaaagt aaaatagacc ataggcaata gattaacaat
      781 taatataata caagcaaata ttctattcat tgtatttctg ctttcaaaaa gttctaatat
      841 tgaaagtata attccacaaa tgcctataat aagtatggga ataagattag aaaataacac
      901 cccgagccaa tctgtgaaaa ttactatatt tactaatatc cctattaaca tacctatcat
      961 atttattaaa tcaatttttt tcattagata tttctccttt attaaatctg aagtacttat
     1021 attaaaataa ttctaaacta tttacctcaa aaagtgcaat aaattatttc tgaacaataa
     1081 aatgatagtc aaaacaaaaa aatattgtta atcgttcaga gccatccgag agtgtaaaat
     1141 attttgtgta aatgaaaaaa tccatacaaa aaaggaaggc gcttctgtag aataaagtta
     1201 acgacaacca attcacagaa aagaggactt ccctatgaat gattttacta cagaaattgt
     1261 gcaaactcta gtcactaaag gcg
//
LOCUS       DAZKKW010000236         1266 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.246, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000236 DAZKKW010000000
VERSION     DAZKKW010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1266
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.246"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            132..506
                     /locus_tag="ACG8PO_002969"
     CDS             132..506
                     /locus_tag="ACG8PO_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFQ0208210.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(620..1249)
                     /locus_tag="ACG8PO_002970"
     CDS             complement(620..1249)
                     /locus_tag="ACG8PO_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFQ0208211.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
BASE COUNT          426 a          179 c          226 g          435 t
ORIGIN      
        1 tctacactct gagatgtaag aaatatcata agatatttct tacatctttt tgttttgagc
       61 aggactttag ataattttat taatatatgc tagcatgctt ttgtaagaaa aagaaagagg
      121 gcgtattagt tatgtatctt aaaattacgg aacaagcaca agaaagatta caaaaatata
      181 ttgatgaagg cgcaacagtt atattggatt tagatgatgg cgttggggaa tattctaaaa
      241 tgggtgtttg ctctttagat actagttttc gtttacttct tcttgataaa gaacagagaa
      301 aaaatgatta taaactccaa ttagatagtg atattgggga tgtttatata aaagattatt
      361 caaaaatgta catggatgaa catatgaccc tttctttaga tccacgtcta ggtgtattta
      421 ctcttgaaag cccaagtggt tctttagaca gtcatgttca attagtagat ctacgaccag
      481 aaaaattctc tggaataaaa caataacttt ctctctattg tccataaaac tagtctcaat
      541 caagctgtat tgaaaaaaca agacaacatt cttaccatgg taaataaaaa gacgacacat
      601 tttgtcgtct ttttttaaat taactatttt cgataaaaga tcgcatagct tgtgccatta
      661 tttctggctt ttctctattc aattcatgtt ttccgttttt aataaagagt agccggccat
      721 tcgttgaaag tttatataaa cgttttgctg ctggtttatt cgcatgatct ttggtaccgc
      781 ataaaatcaa tttaggaagt cgtgatgtac gaagctgttt tttgcagtct aatgtttcaa
      841 gtgaacgaag tagggtaagg gtttgttttt tagatagacc taatttttca aatatcttag
      901 aaggtacgca gtaaaaataa atattctgga aagtaagcaa taagcgatta ggagactcaa
      961 attgtggagc gactaaaaca acctttttta aatatggaga aggagttgca agatagttta
     1021 aagcaataac agcgcctaaa gataatccac ataaaataaa aggttccttt atctgattca
     1081 attgtacttc gatgtatttg gttaagtcgt ctaaagtagc agtgtctgtg ctgtttaaat
     1141 gattgaacaa tgaaagactt ttgcaagaat aactattggg tagataagag attacatctt
     1201 tccatgattg ttcgtcttgg cctagaccat gcaaaaaaac aattttcata ttgctctcct
     1261 ttttat
//
LOCUS       DAZKKW010000237         1266 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.247, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000237 DAZKKW010000000
VERSION     DAZKKW010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1266
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.247"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            290..1135
                     /locus_tag="ACG8PO_002971"
     CDS             290..1135
                     /locus_tag="ACG8PO_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFQ0208212.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
BASE COUNT          481 a          150 c          210 g          425 t
ORIGIN      
        1 tttaaatctt ttgctaagtt gaactaatag agtcttttat gaatatcgaa gttagttttt
       61 cacctaactt cgttttttat agttggttat tataaaatag taatatagct gatttggatt
      121 caattcatat ttatttataa atcaaaaata cagagaatag tatataaatg tcctttccta
      181 ttcttaaaat gatgtttgta cttacttaaa ttaggtataa ttagtatatg ttttgtgaga
      241 aatttagact tacaataagc agtgaattta taatcaacgt ataggagaca tggatgaaat
      301 gaaactaatt ggtatagatt tagacggtac tcttttgaat tctgaccaga gcattagtca
      361 aaaaaatgca agaacaatga aacatttacc ggaagactgt tttccattca tttgttctgg
      421 aagagaagta gaagacatta ataatattct caaaaaatct ggtttaagga taccagcagt
      481 agggttaaat ggttctgttg gatttgatgg agaaaaaaag atatttgagt cttccttcga
      541 taaaaatgat gtaaaaaaaa ttgattctgt cctatcgaaa tttccaacca aaatatatac
      601 aaatctcgga agttatgaat ctcagaatta taaaaataaa atgaaaaaaa tttttcacga
      661 ggtagggagt gaattttcta tcaaagagct aaactatgag cttgagtatg aaaaatctat
      721 ttgttcaatc ccttataata atattgagga aattatagaa aaggatgcta tcagcattta
      781 caaatttttt atttttattc ctaatcgtca attgaaagct aatatattta accatttgaa
      841 aaagatgcta aatgtgtctg ttactgaatc ggcacctgta aatattgaaa tcgtacctca
      901 aaatgtatct aaaggtgtgg tgtttgatcg tttggaatca atctacaatt taaagaaacc
      961 tttgagaatc gcgatcggag atagtttaaa tgacttatct atgtttgaaa gtgcagatat
     1021 tagttttgca atgggaaata gtcatcaaga aatcatgaaa aaagctgatt atgtcactgt
     1081 ttcaaatgat aaagacggag tatctcaagc atttgaaaga attcttacca tataaactaa
     1141 ttaaggtagt atagaacata gaattctaaa tacggaaaaa tggagcataa attccagtac
     1201 aaatagtact tcttcagtgt caaacgaagc gaatataaca tagatattta ggattgtgac
     1261 aaaaat
//
LOCUS       DAZKKW010000238         1264 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.248, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000238 DAZKKW010000000
VERSION     DAZKKW010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1264
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.248"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            134..1006
                     /gene="scrK"
                     /locus_tag="ACG8PO_002972"
     CDS             134..1006
                     /gene="scrK"
                     /locus_tag="ACG8PO_002972"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008865 - fructokinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase ScrK"
                     /protein_id="HFQ0208213.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKLAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMAAGPAIEKRLGKKGQEVAEDDPYWEIEASYIAQCAYN
                     TTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGT
                     LGCLALAREAVLHS"
BASE COUNT          387 a          227 c          289 g          361 t
ORIGIN      
        1 aaaaatgata aaaattcaaa aagaaaaaat agtagttatt tacttgtcat gttactaaat
       61 aaaaaaataa tttaaatatg atatatagtc atttatttag tgatttcatt ctaaaataaa
      121 gggagaaatg tctatgctat acggtgcaat tgaagcaggc ggcacaaaat ttgtgtgtgc
      181 cattggcgat gaagaaatga cgatcaagga aagagtgagc tttccgacaa cgacacctga
      241 ggaaacaatg cctttagtca ttgacttctt caaacaatac caagcagatt tagcagggat
      301 tgggattggc tcatttgggc ctatcgatat ccatcgcgat tccgccactt atgggtacat
      361 tacctcgacc ccgaaattgg catggcaaaa ttttgatttc atcggtacca tgaaaaaaga
      421 atttccgatc ccgatttctt ggacaacaga tgtcaatgcg gctgcctatg gtgagtatgt
      481 cttcggcagt ggcaaaggat tgagcagtgt cgtttactat acgatcggta cgggtgttgg
      541 cggcggtgcc ttacaagaag ggcgctttat cgaagggttt agtcatccag aaatggggca
      601 tatgctcgtc gtccctcatc caaaagatga ctttgctggc agctgtccat ttcatggcaa
      661 ctgtttagaa gggatggccg ctggacccgc catcgaaaaa cgcttaggga aaaaaggaca
      721 agaggttgca gaagatgatc cttattggga aatcgaagcc tcctatattg ctcaatgtgc
      781 ctacaataca accttgatgt tttctccaga tgtgatcatt tttggcggtg gtgtgatgaa
      841 acaacgccat atgctgcaaa acgtccacga cgcatttgca aaattggtca atgggtatgt
      901 cgaaacacct gagttaagca aatatatcgt gaccccagca ttagaagaca acgctggaac
      961 gctcggttgt ttggcattgg ctcgagaagc ggtcttacat tcataaacga aaaaaggctc
     1021 ttcgacattt agtgttggta gagaaagata caactgaaaa tttagtcata accagttagg
     1081 cagagtgctt aactggtttt tgttttactt ggaagatcaa gccttgaatg agataaattc
     1141 ttgctcgaaa gttatggaaa tttcgatagc cgtaggccac ttgtattgtg caatagtttt
     1201 ttacaaaaaa aagggggtgc ggatattttt ttggaccttt ttaacaaata tatctttgct
     1261 tctt
//
LOCUS       DAZKKW010000239         1263 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.249, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000239 DAZKKW010000000
VERSION     DAZKKW010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1263
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.249"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>1263
                     /locus_tag="ACG8PO_002973"
     rRNA            <1..>1263
                     /locus_tag="ACG8PO_002973"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          332 a          285 c          383 g          263 t
ORIGIN      
        1 gtaccgaagt tcctgatgtc acactgccga gaaaagcttc tagtgagaaa acagcggccc
       61 gtaccgcaaa ccgacacagg tagtcgagga gagaatccta aggtgagcga gagaactctc
      121 gttaaggaac tcggcaaaat gaccccgtaa cttcgggaga aggggtgctg atcatacgat
      181 cagccgcagt gaataggccc aagcgactgt ttatcaaaaa cacaggtctc tgcaaaatcg
      241 taagatgaag tataggggct gacgcctgcc cggtgctgga aggttaagag gagtgcttag
      301 cgcaagcgaa ggtacgaatt gaagccccag taaacggcgg ccgtaactat aacggtccta
      361 aggtagcgaa attccttgtc gggtaagttc cgacccgcac gaaaggcgta acgatttggg
      421 cactgtctca acgagagact cggtgaaatt ttagtacctg tgaagatgca ggttacccgc
      481 gacaggacgg aaagacccca tggagcttta ctgtagtttg atattgagtg tctgtaccgc
      541 atgtacagga taggtaggag ccgtagaaat cggaacgcta gtttcgatgg aggcgctggt
      601 gggatactac ccctgcgtta tggccactct aacccgcacc actaatcgtg gtgggagaca
      661 gtgtcagatg ggcagtttga ctggggcggt cgcctcctaa aaggtaacgg aggcgcccaa
      721 aggttccctc agaatggttg gaaatcattc gaagagtgta aaggcagaag ggagcttgac
      781 tgcgagacca acaagtcgag cagggacgaa agtcgggctt agtgatccgg tggttccgca
      841 tggaagggcc atcgctcaac ggataaaagc taccctgggg ataacaggct tatctccccc
      901 aagagtccac atcgacgggg aggtttggca cctcgatgtc ggctcgtcgc atcctggggc
      961 tgtagtcggt cccaagggtt gggctgttcg cccattaaag cggcacgcga gctgggttca
     1021 gaacgtcgtg agacagttcg gtccctatcc gtcgcgggcg ttggaaattt gagaggagct
     1081 gtccttagta cgagaggacc gggatggact taccgctggt gtaccagttg ttctgccaag
     1141 ggcattgctg ggtagctatg tagggaaggg ataaacgctg aaagcatcta agtgtgaagc
     1201 ccacctcaag atgagatttc ccatttcttt aagaaagtaa gatccctgag agatgatcag
     1261 gta
//
LOCUS       DAZKKW010000240         1248 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.250, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000240 DAZKKW010000000
VERSION     DAZKKW010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1248
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.250"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            200..742
                     /locus_tag="ACG8PO_002974"
     CDS             200..742
                     /locus_tag="ACG8PO_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0208214.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            898..>1248
                     /locus_tag="ACG8PO_002975"
     CDS             898..>1248
                     /locus_tag="ACG8PO_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HFQ0208215.1"
                     /translation="MLSEKTDLENTEAFNKESVKDLRKLPTKDINGKDFTSKDFEKYD
                     LTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIVGVVTDPVDDNGENKEAIEKSKL
                     IHEKTKASYPFLMPD"
BASE COUNT          515 a          141 c          238 g          354 t
ORIGIN      
        1 attgatgaag cagatcccaa agggctgctt catcaatttt ttcgtaagtg tccgtacact
       61 tactgtgctt gctcatatat taatcgatca tatactaatc gtgtaagaac aactttaagc
      121 ataatactta tattgttata ccatagtttg gaaaaataaa aaattatatt tcatcaacgt
      181 ttcataaaag gtgtgtagaa tgttattgaa agtagctata aaatgctatt gtgtaaaaaa
      241 taaaggagga ataatttatg aaaaaatagt accaagagga gcatgcctat tacttgcatt
      301 atgtttgagc ttaagcgcag ttgggtgtca aaaaagtaat gaaaacaata aaataaaaga
      361 aggacaatcc ataagttctg aagaggaagg catgtcaatt aatgcaaatg gcaaaaatga
      421 aacttttaag ccatcagatt atacattgga ggcaaagaaa gaatatgttt atgaatattt
      481 agggcttaaa tttaaactct cagacaaatt tagaaattat atagctgata aaaagatagc
      541 gatgttagat gatcaaagtc ctatagataa agaactaaag tatgctattt tgacatttga
      601 gaaaatgaca gaagaacaaa agaatgcagt tatagaaaag atgggcgatg gatataaaaa
      661 ttggcaaaat gagcttgaaa gaattgggac tattggtata tttgaaaaaa atacatcaga
      721 agaaaaaaat ctaaaattat aaaatgtgat actcatacta aaataggagt ttcaagtgat
      781 ggaaaatacg attgctattt aagcacaaat agcggagctg aaagtaatct tctggatgaa
      841 ttaaaaagaa ctgagattca aattatagat aaaaaagaac gtcctaaaaa tggttttgtg
      901 ctatcagaaa aaactgattt agaaaataca gaagcattta acaaagaatc ggttaaagat
      961 ttacgtaaac taccaacaaa ggacataaat ggcaaggatt ttacaagtaa agattttgag
     1021 aagtatgatc ttactatggt aaatgtattt gcaacttggt gtacagcttg tgtaaaggaa
     1081 attcctgatt tggttgaggt tcagaatgaa atgaaaagca agggtgtaaa tatcgttgga
     1141 gttgttacag atcctgttga tgataacggt gaaaacaagg aagcaattga aaagtcaaag
     1201 ttaatacatg aaaagacaaa ggcttcatat cctttcttaa tgccagat
//
LOCUS       DAZKKW010000241         1243 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.251, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000241 DAZKKW010000000
VERSION     DAZKKW010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1243
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.251"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..712
                     /locus_tag="ACG8PO_002976"
     CDS             <1..712
                     /locus_tag="ACG8PO_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HFQ0208216.1"
                     /translation="TPFDEESADFLKQIGVQGFKIGSGDLTNLPFLRKIDMYGLPVLL
                     STGMADIHEVSEAVDCFIDSPVTILHCTSNYPASSEDINLLAINTLKEMFGIPVGYSD
                     HSLGYDVGICAVAMGAKVIEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNTEIFLG
                     DGVKRAMPSELSTKEVSRKSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLYQYIGK
                     KVKRPISADSVLLEEDVE"
     gene            709..>1243
                     /locus_tag="ACG8PO_002977"
     CDS             709..>1243
                     /locus_tag="ACG8PO_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HFQ0208217.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVGAPRIDT"
BASE COUNT          413 a          166 c          270 g          394 t
ORIGIN      
        1 tactcctttt gatgaagaaa gtgcagactt tctaaaacaa attggtgtac aaggatttaa
       61 aattggctca ggagatttaa caaatttacc ttttcttaga aaaatagata tgtatgggtt
      121 gccagtttta ttatctacag gtatggctga tattcatgag gtcagtgagg ctgtagattg
      181 tttcatagat agtccagtta ctattttaca ttgtacttca aattatccgg ctagttctga
      241 agatattaat ttattggcga ttaacacatt aaaagaaatg ttcgggatac ccgttgggta
      301 ctccgatcat tcattgggat atgatgttgg tatttgtgct gttgcgatgg gtgctaaggt
      361 aattgagaaa cattttacct tggataagga actacctggt ccagatcata aagcttcttt
      421 agatcctcag gaatttgcag attttgtaaa gcatattaga aacactgaaa tatttttagg
      481 tgatggtgtt aagcgagcaa tgccttctga acttagtaca aaagaagtat caagaaaaag
      541 cctagtggtc actgagaatt taaatgtggg agatattctt acagagaaat ctcttacaat
      601 aaaacgtcct ggaaatggaa tagctcctaa gtttttatat caatatattg gcaaaaaagt
      661 taaaagacca ataagtgcag atagtgttct gcttgaggaa gatgttgaat gaaaaaaata
      721 cttatagtaa gtggaacacg agctgattat ggaatatata aacctgttgc tgagaattta
      781 ctacaagata attgggatgt atcatttgtt gtttctggaa tgcatttatc ccataaatat
      841 ggatttacta aagaattgat cttagctgac ggatttagaa ttagtggaga agttcaaagt
      901 ttgtttttag agaacaataa gggaaacatg gctcggtctg tggcattaga gataaatggt
      961 ttcacaaata tatttgaaca aactagccct gattttgtat tattactagg ggatagagga
     1021 gaaatgttgg cagctgcgat agcttgtttg tatcttggaa tcaaaacagc acatattcac
     1081 ggaggcgaag tgtctggtac tgtagatgaa tcaataagac atgccataac gaaaatagca
     1141 tctgttcatt ttgctgctac ggaaaaaagc aaagagcgtt taataaaaat gggggaagat
     1201 tcttggagag tttttcccgt cggagctcct agaatagata cta
//
LOCUS       DAZKKW010000242         1220 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.252, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000242 DAZKKW010000000
VERSION     DAZKKW010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1220
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.252"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            18..1025
                     /locus_tag="ACG8PO_002978"
     CDS             18..1025
                     /locus_tag="ACG8PO_002978"
                     /EC_number="4.2.1.115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050377 - UDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 4,6-dehydratase family
                     protein"
                     /protein_id="HFQ0208218.1"
                     /translation="MFKGKTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFY
                     MQQELSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNV
                     MGTQNVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIF
                     SCVRFGNVMGSSGSVIPLFSKQIKNNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSK
                     FGETYVLKMPAINILTLAQSLIKIYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTDEI
                     ERSYENDKMYIIPPHYHATDELYKDYEKIKITAYSSDATELMSGQEFIRIAKENNVVT
                     W"
     gene            1037..>1220
                     /locus_tag="ACG8PO_002979"
     CDS             1037..>1220
                     /locus_tag="ACG8PO_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HFQ0208219.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQKDN"
BASE COUNT          481 a          158 c          234 g          347 t
ORIGIN      
        1 cataggagga gatataagtg tttaaaggaa aaacaatatt agtaactggc ggaactggtt
       61 ccattggaag tgaaattgtt cgtcagcttt taaaatatga accaaaagct atcagaattt
      121 atagtagagg tgaagaaaaa caattttata tgcaacaaga gctctcaaat cacactaatt
      181 tgagattttt aattggtgat atcagagact ctcaaaggat tgattatgca tgtcgtgatg
      241 tggatattat ttttcatgct gccgcaatga aacacgttcc agcaagtgaa tataatccta
      301 tggaagctgt aaaaacaaat gtgatgggaa ctcaaaatgt aatagatgca gcgatcaaaa
      361 ataatgtaaa acattttgta ggtatcagta cagataaagt agtgagtcct actaacacca
      421 tgggtgcgac aaagttattg agtgaaaaat taataatagc tgccaatcag tataaaggaa
      481 aaacagaaac aatattttct tgtgtaaggt ttggaaatgt catggggtca agtggatctg
      541 ttattcctct ttttagtaaa caaataaaaa acaataaaga tattactttg acagatgttg
      601 atatgacacg atttatgatg tcaataccgg atgctgcaag attagttctg tctgctgcta
      661 tgaattcaaa atttggagaa acatatgtct taaaaatgcc ggcaatcaat attttaactt
      721 tagctcaatc attaataaaa atatataatg atacacatga tgaaaaatat agaggtgaaa
      781 taaaagttgt aggtatcagg ccaggagaaa aattatatga agagctaatg actacagacg
      841 aaattgaacg ctcatatgaa aatgataaaa tgtatatcat tccaccacac tatcatgcta
      901 ctgacgaact gtacaaagat tatgaaaaaa ttaaaatcac tgcatactct tcagatgcaa
      961 cagaattaat gagtggacag gaatttatta gaatcgcaaa agagaataat gtcgtgactt
     1021 ggtaaggagt tttatgatga aatcagaagt atatgtaatt gcagaaattg gtgtaaacca
     1081 taatggaagt ctagatttag ctttagaaag tattgataaa gctaaagaat gtggagctaa
     1141 cgcggttaaa tttcaaacat ttaaaacgaa taagctagtt tccaaaaagg ctccaatggc
     1201 aaaatatcag aaggataatc
//
LOCUS       DAZKKW010000243         1207 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.253, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000243 DAZKKW010000000
VERSION     DAZKKW010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1207
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.253"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..588
                     /locus_tag="ACG8PO_002980"
     CDS             <1..588
                     /locus_tag="ACG8PO_002980"
                     /inference="COORDINATES: protein motif:HMM:NF015839.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF334 domain-containing protein"
                     /protein_id="HFQ0208220.1"
                     /translation="SEQDRNERTNILMKRLEASTSNFNNKLDVFEEKTKHARLNFETT
                     AKHYIKRLDEDNLKLDFQQAIQDELSDTKDEIREVTKQAREETKEYKEILESKIKDHN
                     KVVDKSNTALKVMTKGVTNIFFVLIIFVLVMLVTGPIGHFFGIEHLYSFINGFIDDHE
                     SAWRYLMLILYAVPYVFFAIILWLIALFFRVFNDL"
     gene            883..1056
                     /locus_tag="ACG8PO_002981"
     CDS             883..1056
                     /locus_tag="ACG8PO_002981"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0208221.1"
                     /translation="MSEKTKKIIRVSLAFSQVVVQSLIVYYTYQQYKWFCCKVFQKIY
                     FSVKLRKKTERTE"
     gene            1056..>1207
                     /locus_tag="ACG8PO_002982"
     CDS             1056..>1207
                     /locus_tag="ACG8PO_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HFQ0208222.1"
                     /translation="MNHFKGKQFKKDVIIVAVGYYLRYNLSYREVQELLYDRGINVCH
                     TTIYRWV"
BASE COUNT          453 a          165 c          233 g          356 t
ORIGIN      
        1 agcgagcaag acaggaacga gagaacaaac atactcatga aaagactaga ggcttcgacc
       61 tcgaacttta ataataagtt agatgttttt gaagaaaaga ctaagcatgc aagattaaac
      121 tttgaaacaa ctgcaaagca ctatattaaa cgacttgatg aagataattt gaaattagat
      181 tttcaacaag ctattcaaga tgaattaagc gatactaaag acgaaatacg tgaagtgacg
      241 aaacaagcga gagaagaaac aaaagagtat aaagaaatct tagaaagtaa aataaaagac
      301 cataacaaag tggttgataa atcgaataca gcgctaaaag tgatgacaaa aggcgttaca
      361 aatatatttt ttgtgctaat tatttttgta cttgtaatgc ttgtgacagg tccgataggt
      421 catttctttg gtattgaaca tttatatagc tttattaatg gttttattga tgaccacgaa
      481 agtgcatggc gatatttgat gttgatactt tatgcagtac cctatgtatt cttcgctatc
      541 attctgtggc tcatagcatt gttttttaga gtatttaatg acctttaggt agatttatat
      601 ataaaaaggg ctaaatacgc aaaatacccc cttaaaatgc aaaataaatg caaataaggg
      661 ggtaactcct tatgaaaaaa caactaatag tattataaca cgtttttatg ccgagaaaac
      721 ttattggttg gaatgggcta tgtgttagct aacttgttag cgagttggtt ggacttgaat
      781 tgggattaat cccaagaaag taccaactca acaacacata aagccctgta ggttccgacc
      841 aataaggaaa ttggaataaa gcaacgaaag gagttgagta aaatgagtga gaaaacgaag
      901 aaaatcattc gagtatcatt agctttttca caagtagtag tgcaatcatt gatagtttat
      961 tatacttacc agcaatataa atggttctgt tgcaaagttt tccaaaaaat ctattttagt
     1021 gtaaaattga gaaaaaagac agagaggaca gagtaatgaa tcattttaaa ggcaaacaat
     1081 tcaaaaaaga cgtcattatt gtcgctgttg gttactacct gcgttacaat ctaagctatc
     1141 gtgaagttca ggaattgtta tatgatcgtg gaataaatgt ttgtcatact acgatttatc
     1201 gttgggt
//
LOCUS       DAZKKW010000244         1207 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.254, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000244 DAZKKW010000000
VERSION     DAZKKW010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1207
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.254"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            123..1013
                     /locus_tag="ACG8PO_002983"
     CDS             123..1013
                     /locus_tag="ACG8PO_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HFQ0208223.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
BASE COUNT          436 a          220 c          260 g          291 t
ORIGIN      
        1 taaagaatgc gaaaaacgtt tacatgattt ttacataact taactatatt tttacagcaa
       61 ccatacgagg gatttacagt cgactgataa actttagttg taaataagaa aggagcaata
      121 aaatgaaaaa agtattgttt actctaggta tgatgggaat tcttttagcc ggatgcggct
      181 caggatcagg aaatacagat ggctctgcca cacaaaataa ttcaagtgac tcaaatgaac
      241 aagtaaaaat cgttgcggta ggttcgacag cattacagcc gttagtcgat gcagcacaag
      301 aaagctttgt acaagaaaat ccaaattatc aaatctctgt acaaggagga ggaagcggaa
      361 ctggtttgag ccaagtcgaa gctggagcag ttactatcgg aaactctgat gtatttgcag
      421 aagaaagaga cggagttgat gcttcaaaat tagtggatca taaagtagca gtggtcggga
      481 tggcaccaat cgtcaataaa gatacagatg tcaaagatat caccaaacag gaattgattg
      541 atatctttac aggaaaaatc actaactgga aggaagttgg cgggaaagat caaaaaatca
      601 acgtcgtgaa ccgtgcaaac ggcagcggta cccgcgcgac attcgaaaaa tggggactgg
      661 atggtgctac ccctgtgcag tcccaagaac aagattcatc aggaacagtt cggcagctgg
      721 taagtcaaac accaggagca atcagctatt tagctttctc atatctcgat gattccacac
      781 aagcactaag tatcgacggt gtagaaccaa aagaagagaa tgtagcagat aacagctggg
      841 gaatctggtc atatgaacat atgtacacga acggaaaacc ttcccctgaa gtccaaaaat
      901 tcttagacta catgatgaca gaagaaatcc aagaaggacc tgtcaaagaa ttgggctatt
      961 taccaatcac aatgatggaa gtagaacgtg atcacgaagg aaatatcaaa taactagcag
     1021 ataaacatgc cacggtagtc gttactatta aggaaaaatc aactatgttt ccccacatat
     1081 acatttttcc tttaattgtg aaaaatagtt cctcatattt tgccgttttt tgagctaata
     1141 ccgagggtca gctgattgaa caccgtttat tcgaaaaaaa gagctacgac atagaactgt
     1201 cgtaact
//
LOCUS       DAZKKW010000245         1195 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.255, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000245 DAZKKW010000000
VERSION     DAZKKW010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1195
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.255"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(89..415)
                     /locus_tag="ACG8PO_002984"
     CDS             complement(89..415)
                     /locus_tag="ACG8PO_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0208224.1"
                     /translation="MAEDNRSQAAVSRLLGNKNKKNFKSKEVEEIELTKKNISKVIKD
                     QGSSVRTADSIRINPILMSALKYWTTIAESDKSKPDVIEDALLKSIPEEYLIEGYKLA
                     KKQNKI"
     gene            complement(402..1190)
                     /locus_tag="ACG8PO_002985"
     CDS             complement(402..1190)
                     /locus_tag="ACG8PO_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0208225.1"
                     /translation="MARILSVANQKGGVSKTTTSVNLAACLKFVYGKNVLLVDIDPQG
                     NATDNVGFDIDGTNQPTIYEVLKGEADITDAILDYKGIDVLPADIALSSAEREFTQVG
                     SEHRLKRVLQPIKDNYDYIIIDCPPSLGMLTVNAFTVSDEIIIPVEAAYFSLKGLIKL
                     SETIETVKEYTNDKLEVRGVLFTKYNKRYNISKEMTSAATEISRVIGAKIFKTYIRRT
                     ITVDEAQAAGSDLINFNKPSTAEDDYKAFTEEYLKEVGDFDGRR"
BASE COUNT          337 a          217 c          174 g          467 t
ORIGIN      
        1 tgaagataag cgacttatca gctttgatac tactgatttt tataaaaaaa atatcacgat
       61 attatgatat cgtgatattg aatgtaaatt aaattttatt ttgtttcttt gctagtttat
      121 atccttcaat caagtattct tcaggtatgg actttaatag cgcatcttct ataacgtctg
      181 gtttactttt gtctgattca gcaatagtcg tccaatattt gagtgctgac attagaattg
      241 gatttatacg aatgctatct gctgttctta cactacttcc ttgatctttt atgactttac
      301 ttatattttt cttggttaat tcgatttctt ctacttcttt tgatttaaaa ttctttttat
      361 ttttatttcc tagtaatcta cttactgcag cttgactgcg attatcttct gccatcaaaa
      421 tcaccaactt cttttaaata ttcttctgta aatgctttat agtcatcttc agctgttgag
      481 ggtttattaa aattgattag atctgatcca gcagcttgcg cttcgtcaac ggttattgtc
      541 ctacgaatat aagttttgaa aatttttgca ccaatgactc tagaaatttc ggttgctgca
      601 gacgtcattt ctttagaaat gttataacgc ttattatact tagtaaatag aactcctcgt
      661 acttcaagct tgtcgttcgt atattctttt accgtttcaa tagtttcact taactttatt
      721 agtcctttta atgagaaata cgcagcttca actggaatga taatttcatc agaaacagta
      781 aatgcgttga ctgtaagcat tcctaaactt ggtggacaat caattataat ataatcatag
      841 ttgtccttga ttggttgaag tactctttta agcctgtgtt cacttcctac ttgagtaaat
      901 tccctctctg cagaagaaag ggctatgtcg gctggcaaaa catcaattcc tttatagtcc
      961 agaatagcat ctgtaatgtc agcctcccct tttaatactt cataaattgt aggttggttt
     1021 gttccatcta tatcgaaacc tacattatct gtagcatttc cctgaggatc aatgtccact
     1081 aaaagtacat ttttaccgta aacaaatttc aaacatgctg ctaaattaac gctagtcgta
     1141 gttttagaaa ctccaccttt ttgattggct actgacaata ttcttgccat taaaa
//
LOCUS       DAZKKW010000246         1173 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.256, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000246 DAZKKW010000000
VERSION     DAZKKW010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1173
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.256"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..456
                     /locus_tag="ACG8PO_002986"
     CDS             <1..456
                     /locus_tag="ACG8PO_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0208226.1"
                     /translation="AERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQEH
                     KNTVETAENKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGKS
                     YGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            517..>1173
                     /locus_tag="ACG8PO_002987"
     CDS             517..>1173
                     /locus_tag="ACG8PO_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0208227.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDSKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKYERQNF
                     LVRDDRLDHAVAFLWKDPQTKETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     L"
BASE COUNT          455 a          180 c          252 g          286 t
ORIGIN      
        1 gcggaacgtg ggaaaatcat gaatgatgtc agccgtgtcg caaatgaatt cattcaaacc
       61 atttctaatg aaatcaatca atacagagaa agagaaccag agatccaacc agcagaagtg
      121 aagcaggagc ataaaaatac tgtagaaacg gcagagaata agaatattgt gcccgttggt
      181 tcactttgga tcgacaagaa agacgggaaa gtaatggaag aagatacggg tcgaacgtta
      241 catttgaagg atgcggcgtt tattgaagat ccgcagcata atcaagtgat tttaaaagga
      301 aaaagttatg gaaaagagat tcaaacggcg ttaccttcag aagaatttgc gctaggaaat
      361 ccattccgat tttcacttga aacagtgtca gaagtggcca aacgaaaaaa tgaaatctat
      421 tatgaacaac aattgtcaca aaaagaacgc gtataaaaca agtaaaaaag actatctgaa
      481 aaacggatag tcttttttct tttgggaggt ttgaaaatga ccgaaaaaaa aatgagtctt
      541 attgatcgat gtaaacagat cgatatagtg gattttgctc gaaacaatgg aatggctgta
      601 gtcaataaag gacgagacta tcgcttagaa gaccatgatt catttgtttt tgatcgaaga
      661 aaacaacgtt tttattggaa cagtcaaaat attagtggcg acattattga attagctaaa
      721 ctttttttca tagacaaaga gatccaagac tctaagcaac aatttaaagc agccactgat
      781 tttattctaa agaatgaaga caaaacagaa cgagtggaaa atcttcattt tgaaacagaa
      841 aagtacaaag atcaccccgt tgattatcaa ccactcacag aaaaaggaag gaattatctg
      901 aaggaagaac gaaaactacc agagtggctg attgattatg cagaaaaaga agggttgatt
      961 gccgaactaa agccaaaata tgaacggcaa aatttcttag ttcgagacga tcgtttagat
     1021 catgcggtcg cctttttatg gaaagatcca cagacaaaag agacagtagg ggcgagttat
     1081 caaggaacgt tcattgatta tgaacgtttt ggtgagagag gcacatataa gcatatagat
     1141 aaaaattcga cagcgaatca tggctttaac cta
//
LOCUS       DAZKKW010000247         1170 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.257, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000247 DAZKKW010000000
VERSION     DAZKKW010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1170
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.257"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..345
                     /locus_tag="ACG8PO_002988"
     CDS             <1..345
                     /locus_tag="ACG8PO_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HFQ0208228.1"
                     /translation="VNMLLILSQSGDEYYHEHFPELTFYRNGPYSIDTVKKGISKGAG
                     VKRLQKELQLEKVPTFGFGDGPNDFALLEACDNKIAMDNAYDGLKELSTFITKKNTEG
                     GIVHALKYFDLI"
     gene            516..882
                     /gene="ssrA"
                     /locus_tag="ACG8PO_002989"
     tmRNA           516..882
                     /gene="ssrA"
                     /locus_tag="ACG8PO_002989"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
BASE COUNT          374 a          198 c          228 g          370 t
ORIGIN      
        1 gtaaatatgt tgctgatctt atctcaatca ggtgacgaat actatcacga acattttcct
       61 gagctaactt tttatcggaa tggtccttac tcgatcgata cagtgaagaa aggaatctca
      121 aaaggggcag gtgtcaaacg tctgcaaaaa gaacttcagc tggaaaaagt tcctactttt
      181 ggatttggcg atggtccgaa tgattttgcc ttgttggaag cttgcgacaa caaaatcgct
      241 atggacaacg cctatgacgg attaaaagaa ctttctacct ttatcacaaa gaaaaataca
      301 gaaggcggaa ttgtccacgc attgaagtat tttgatctga tttaagatct ttttttgaag
      361 ctacctcttt tcttaagaaa atcgcttatt ttttcttaag aaaggggcag ttattatata
      421 aataatggga tatgattcgc ttttttattg aatttatccc aatatatgct atactaattt
      481 tgtatgaaaa atacacgtaa cccttgttag ctaacgggga cgttacggat tcgacaggca
      541 cagtcgagct tgaattgcgt ttcgtaggtt acgtctacgt aaaaacgtta cagttaaata
      601 taactgctaa aaacgaaaac aactcttacg ctttagctgc ctaaaaacag ttagcgtaga
      661 tcctctcggc atcgcccatg tgctcgagta agggtcctaa ctttagtggg atacgtttca
      721 actttccgtc tgtaagttga aaaagagaac atcagactag cgatacagaa tgcctgtcac
      781 tcggcaagct gtaaagtgaa tccttaaatg agttgactat gaacgtagat ttttaagtgg
      841 cgatgtgttt ggacgcgggt tcgactcccg ccgtctccat atatgtatgg ttgagacaga
      901 taaacatgta tgaaaaacgt tgttaaatca acgttttttt attttggtca aggagagaaa
      961 agtagagaaa tacaaaattt tttgcattga ttttgcattc aaaaaaacat tcaaacattt
     1021 agaaatcccc aatacgtatt caggaaaatc tattttaagg tttacaaaaa gaacttttgt
     1081 tcgtacactc tttttgaggt gaacttttat gctgatggaa ggaaaaacac aattatggtt
     1141 taagcatgtt ccttcaaata agttttttta
//
LOCUS       DAZKKW010000248         1156 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.258, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000248 DAZKKW010000000
VERSION     DAZKKW010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1156
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.258"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            114..1133
                     /locus_tag="ACG8PO_002990"
     CDS             114..1133
                     /locus_tag="ACG8PO_002990"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFQ0208229.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
BASE COUNT          405 a          183 c          260 g          308 t
ORIGIN      
        1 gtaaaaacga taaaagaagt aaacaaacac ttaaaaacaa tggaaattag tcccaacttt
       61 aagaaatgta tactataatg gaaattatca attatttatt tggaggaata tccatgaaac
      121 aaaaaatcgc agttcttggt cctggatctt ggggcactgc tttagcacaa acattggcag
      181 aaaacgggca tgatgtccgt atttggggaa atgtacctga acagattgat gaaatcaata
      241 agtatcatac aaatcagcat tttttacctg atttgacgat cccagaatcg attatcggat
      301 ataaagaatt ggcagaagca gtggataacg cagatgctat tttatttgtt gttccaacaa
      361 aagcgattcg ttcagtcgca caggaactta ttggtaaaat gaatactaag ccaatcatta
      421 tacatgcaag taaaggacta gaacaagata cgcataaacg aatctcagaa gttttggctg
      481 aagaaattcc agaagaaaaa agacaggcaa ttgttgtatt atctggtcct agccatgctg
      541 aggaagtggc agtacatgat atcacaacaa ttacagctgc aagtattgat caaaaagcag
      601 cagcctatgt acaaaaactg ttcatgaatg attatttcag gatttataca aataatgatg
      661 tgattggcgt agaaactggt gcagcactga aaaatatcat tgcaattggt gccggcgcta
      721 tccatggatt aggattcgga gatgatgcaa aagcagcaat catgactcga ggattggcag
      781 aaatcagccg tcttggtgtc gcgatgggtg ccaatcctct gacatttatc ggtttgagtg
      841 gtgtggggga cttgatcgtt acttgtacaa gtgttcattc tcgcaattgg cgtgccggta
      901 acttgttagg acagggacat aagctggaag aagttctaga aaacatggga atggtcgttg
      961 agggtgtggc aacgacaaaa gcagcagtag agctagctca acagctaggt gttgagatgc
     1021 caattactca aacaatctat aatgttttgt ataatggaga agatatcaaa aaagcagcaa
     1081 aagaaatcat gttacgtgac ggaaaaatgg agaatgaatt ttctcttcgt taaatagatg
     1141 atcaatagtt aatcgt
//
LOCUS       DAZKKW010000249         1155 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.259, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000249 DAZKKW010000000
VERSION     DAZKKW010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1155
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.259"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            42..857
                     /locus_tag="ACG8PO_002991"
     CDS             42..857
                     /locus_tag="ACG8PO_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HFQ0208230.1"
                     /translation="MKVLGLGDNVVDKYENLGIMYPGGNSLNFAVFAKKLGHESSFMG
                     VFGSDEEAAHVLSAIKEIGLDNSHSRFEKGENGCARVKIDEGDRIFLGSNSGGVTREY
                     PIQLTEEDREYLNQFELIHMGLYSHVNHLLEELQNVSGKISYDFSDDFTEEEVQRAID
                     KVDFGFFSIEDFSDEEVKQFLMKHFCERNDVLIATRGEKTAIGYDGAKFYEVNPVLEE
                     PVDTMAAGDSFLTAFLLYFLEGDDIVTAMKKGNEFAGKSCLVDGSFGFGIHYD"
     gene            925..>1155
                     /locus_tag="ACG8PO_002992"
     CDS             925..>1155
                     /locus_tag="ACG8PO_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0208231.1"
                     /translation="MLDTNAQLPLYLQLKEILKNKINTGEYPENTKIPAESELIESYE
                     VSRITVRKAVEELVEEGYLLKKQGKGTFVNRHK"
BASE COUNT          385 a          165 c          281 g          324 t
ORIGIN      
        1 tcgtcgctat atggggaaag tggagtacta ggaggattaa aatgaaggta ttaggcttag
       61 gtgataacgt cgtcgataaa tatgaaaatc tgggaattat gtatcctgga ggaaattcat
      121 tgaattttgc agtttttgca aaaaaactgg gacatgaaag tagctttatg ggagtttttg
      181 gctcagatga ggaggcagct catgttttat cagcaataaa agaaatagga ctggacaaca
      241 gtcactctcg ctttgaaaaa ggcgagaatg gctgtgccag agtgaagatt gatgagggcg
      301 accggatttt ccttggcagc aattcgggag gtgttactcg tgaatacccg attcagctta
      361 ctgaagaaga cagagaatat ttaaaccagt ttgaattgat tcatatggga ttatatagcc
      421 atgtcaatca tctattagaa gagttacaaa atgtttctgg aaagatttct tatgattttt
      481 ctgacgactt tacggaagaa gaggttcagc gggcaattga taaagttgat tttggcttct
      541 tttctataga agacttttcc gatgaagaag taaagcagtt tttgatgaag catttttgtg
      601 agcgaaatga tgtattgatt gcaacccgag gagaaaaaac ggctattggt tatgatggag
      661 caaagtttta tgaagtgaac cccgtgctag aagaaccagt tgatacaatg gcagcgggag
      721 attctttctt aacagctttc ctactttatt ttctcgaagg ggacgatata gtcaccgcga
      781 tgaaaaaggg gaatgaattt gctgggaagt cttgtctggt tgacggttcg ttcggctttg
      841 gtattcatta cgattgagct tgttttaaat tcctgcattt gataacctaa agaaagaaat
      901 cccggtaaag tggaggttaa aaaaatgctg gatacaaatg cacaattacc attgtactta
      961 caattaaaag aaatattaaa aaataaaatc aatacagggg agtatcccga gaatacgaaa
     1021 attcccgctg aatcagagct gatagaaagt tatgaggtca gccgaatcac agtgagaaaa
     1081 gcagtagaag aactggtaga agaagggtac ctacttaaaa aacaagggaa aggaactttt
     1141 gtcaaccggc ataaa
//
LOCUS       DAZKKW010000250         1148 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.260, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000250 DAZKKW010000000
VERSION     DAZKKW010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1148
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.260"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            221..490
                     /locus_tag="ACG8PO_002993"
     CDS             221..490
                     /locus_tag="ACG8PO_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFQ0208232.1"
                     /translation="MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKR
                     DYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTHDLIEVESDD"
     gene            483..740
                     /locus_tag="ACG8PO_002994"
     CDS             483..740
                     /locus_tag="ACG8PO_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFQ0208233.1"
                     /translation="MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLG
                     KPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYSAKDHY"
BASE COUNT          442 a          129 c          206 g          371 t
ORIGIN      
        1 aaaagtgaat ccaggattat gtgtattgcg ttagatttat tgattgccca agaaaaagca
       61 ggatttgagg taccagcact caaaaagtga cgtcactttt tatcctaaaa actaaaagtg
      121 atagcacttt taattataag aagttagaat attaatcatt tgcttaattg tacaatataa
      181 tgtacaattg ttttatagaa ataaataagg ggtgaaagga atggaagcag tagcttattc
      241 aaatttccgc caaaatttac gtagttatat gaaacaagtt aatgaggatg ctgaaacact
      301 tattgtaaca agtaaagatg tagaagatac agttgttgta ttatcaaaaa gagattatga
      361 ttctatgcaa gaaacgttga gaacactttc taataattac gtcatggaaa aaattcgtcg
      421 aggagatgaa caattctcca aaggtgcatt taaaacacat gacttaatcg aggttgaatc
      481 tgatgattaa ggcttggtct gatgatgctt gggatgatta tctttattgg catgagcaag
      541 gaaacaaaag caatataaaa aagattaaca agttaataaa agatatcgat cgttccccct
      601 ttgctggatt aggaaaacct gagccattaa agcatgattt atctggaaaa tggtccagaa
      661 gaattacaga tgaacataga ctgatatata gagttgaaaa tgaaacgata tttatttatt
      721 ctgcaaaaga tcactattaa ccaatcggaa gtaaggaaag ggtcagaaac ttaaaagttt
      781 ttgatcctta ttttatttac cctagtcatt taaaaagcta atatagctta gtgttgattg
      841 ttattaatga atgtgtttgt tacgcgtatt acggatataa ggttagtaaa atcatttcta
      901 aagttgagga aaagtaaata taaatggctt aaatttcaac aatttgaagt tgaatagata
      961 tgttataata ctattgtagt gtgggatgtt agttactaaa ggatgacgct tatatatatg
     1021 actgaataga ataagcaata ggtttaataa tctattttaa attttttgta ctagttttag
     1081 ttaattagca aaaacaacaa aaataaactt ctcatagaat ttagctaaaa attaatgatt
     1141 tatttaca
//
LOCUS       DAZKKW010000251         1142 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.261, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000251 DAZKKW010000000
VERSION     DAZKKW010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1142
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.261"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            278..595
                     /locus_tag="ACG8PO_002995"
     CDS             278..595
                     /locus_tag="ACG8PO_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HFQ0208234.1"
                     /translation="MLFYSNFILIVAILLLLNIWIFDRSRNSSIGFRTKRSLSSKKNW
                     VYSQTIFYGGIVLISLLSSTLYSLNIIDVSTSNSISIIGIIIAAIITQLFLVFGEKKR
                     SKK"
     gene            complement(686..1051)
                     /locus_tag="ACG8PO_002996"
     CDS             complement(686..1051)
                     /locus_tag="ACG8PO_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0208235.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
BASE COUNT          415 a          147 c          182 g          398 t
ORIGIN      
        1 agtatcgtat ttcgcaatct taaaattatc ttcttactgt gaataagata ataaaatttg
       61 tatttgtcct ttttaggtaa cttattttaa tctatatacc tttctggatc aacgaaagta
      121 tactttatat agtcataacg ccgatggtaa ctatgcactg taacgataat gctgctaaaa
      181 tatcttttta cgaaaaacat cacaaggaaa aacttaacta tggtattatc taaacataat
      241 tttttcggta tttaatattt aattaggagg taatattttg cttttttata gtaattttat
      301 cttaatagta gcgatattat tacttctgaa tatatggatt tttgatagga gtagaaattc
      361 aagtataggt tttagaacta aacgaagttt gtcttctaaa aaaaattggg tatactcaca
      421 aactattttt tatggaggaa ttgttttaat tagtttactt tcttcaacac tttattcttt
      481 gaatattata gatgtttcca cctcaaattc aatatctatt attggaatca ttattgctgc
      541 aattattaca caactgtttt tagtgtttgg agagaagaaa agaagtaaaa aatagaaaaa
      601 ctagtgatag ttttggtgat ataagaaaaa catatatgta taaaaagaag aaagtagctt
      661 ctctttgtac atatatggtt tgtttttatg gatgccgagc ttttttataa gataaggcta
      721 ccgcccataa cgacccaagg atttgtggaa ctaagtaaat acctgcaaga agcaagatca
      781 gctgggtcgg ttttaaagga tgatagaagt aacgccattc agtaaaattg gcgcaaaata
      841 aataggaagt gacagtagat ataatattgg caatgaataa agatataata cgcctgttcc
      901 tttgaaaata atatccagca aaaagagata aaaatatcaa tgtgatgaac ccgcttacgg
      961 aatgataacg aaaatcaagc agtaaggcga aatgcacata aggaacaaaa gataaaagca
     1021 acggaaggtt tttccagcaa gtttttatca tctttcatca ccacctaaca agtgttatta
     1081 ttttgttagt tttattgtaa attgaatcct gcaataatca attacttttt cattatttct
     1141 ag
//
LOCUS       DAZKKW010000252         1128 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.262, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000252 DAZKKW010000000
VERSION     DAZKKW010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1128
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.262"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            18..491
                     /locus_tag="ACG8PO_002997"
     CDS             18..491
                     /locus_tag="ACG8PO_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HFQ0208236.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            500..727
                     /locus_tag="ACG8PO_002998"
     CDS             500..727
                     /locus_tag="ACG8PO_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0208237.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
BASE COUNT          357 a          185 c          194 g          392 t
ORIGIN      
        1 ataaaagagg gataaaaatg aaatttaaaa cattgctatt aaaatttgtc gttttaggta
       61 taagtgccgt atttattttg ttcggcgtac ttttttatac tcagatcgct acaagcgaaa
      121 aaggattcaa ttttgattgg cagaccacat tatttgtttc tgtcatttac tttgcccttc
      181 tcctagggat cgttattgct tatttcttat tgaaactatt gaaaaaaatc gaagataacc
      241 aagctttttc cactcaaagc ttaaacatac taaaaaaaat cgaacgaacc attttgttta
      301 ttggtttcgc cttttttggc ttacttccta aatttatgaa agtagcggat ttagacgatg
      361 ctccaggttt gatgttggta ggaatcgctg tcgtattcat tccttttgca gtatacacat
      421 taacaagcgt gctggaaaaa ctgcttgaac atgcgatttt acttaagaca gatcatgatt
      481 tgacagtgtg aggtgcgaca tggcaattat cgttaattta gatgtagtat tagctcaaaa
      541 aaaaatgagt gtgactgaat tgagtgagaa agtgggcatt acgatggcaa acatctctat
      601 tctaaaaaat ggaaaagcaa aagccatccg tttttccact ctcgaaaaaa tctgccaagt
      661 tttggagtgt caaccaggtg atttgttgac ctataaaaat gactcgaata ctataccaga
      721 agaataaaat aaagtagaac aaacagtaaa taaaaaataa tctgaatgat agatgtgtga
      781 gacttttaga agttatttac gtctattatt cagatttttt atttcgattg aataccgttt
      841 tatctataaa taagatatgt actgactcat acaaaggggg ccttttcttc tccatgtact
      901 atcacttttc ctgatacaac tggtttcctg ttacgttgta gttggatctg cttttagtat
      961 aatactttat tcattccacc agactgatag cagtttgagc agtctaaata atcatgtagt
     1021 attacaggcc attctgtttc ataccttgaa gtgcttaaaa cttcagctaa tggcccattg
     1081 tgttaactct tactccattc ttttttagta agctcccata tatactgt
//
LOCUS       DAZKKW010000253         1097 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.263, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000253 DAZKKW010000000
VERSION     DAZKKW010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1097
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.263"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(83..>1097)
                     /gene="uidA"
                     /locus_tag="ACG8PO_002999"
     CDS             complement(83..>1097)
                     /gene="uidA"
                     /locus_tag="ACG8PO_002999"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HFQ0208238.1"
                     /translation="VIDTYTEEFGIRSVQVTNGKFLINNKPFYFKGFGKHEDSYVNGR
                     GLNEAVNIKDFNLMKWIGANSFRTSHYPYSEEIMRLADREGIVVIDEVPAVGLHLNFM
                     ATLLDDNVEKHNTWKEIRTENSHKTVIKELIERDKNHPSVVMWSIANEPDSDSEGAKE
                     YFEPLVELTKELDPQKRPVTIVTYLKSTPDVCKVGDIVDVLCLNRYYGWYVSGGDLAH
                     AKYLLKKELDGWMERCPDTPIMFTEYGADTVAGFHDTVPTMFTEEYQVAYYEANHEVI
                     DTCENFVGEQVWNFADFATSQGILRVQGNKKGIFTRDRKPKMIAYYLQKRWSSIPNFS
                     YKK"
BASE COUNT          345 a          228 c          137 g          387 t
ORIGIN      
        1 aacagaaaat ctttctataa aaaacaaaaa aaaacgatta ggctaggcta atcgtttttt
       61 gaacattctt aaaaaatcca aattattttt tataactgaa attgggtatt gatgaccatc
      121 gtttctgtag atagtaagca atcattttag gttttctatc tctagtaaag attcctttct
      181 tatttccttg cactcttaaa atcccttgac ttgttgcaaa atctgcaaaa ttccacactt
      241 gttcaccaac aaaattttca catgtatcta ttacttcgtg atttgcttcg taataagcca
      301 cttgatactc ttctgtaaac attgttggta ctgtatcatg aaatccagct actgtatctg
      361 ctccatattc tgtaaacatt attggcgtat caggacatct ttccatccat ccatctagct
      421 cttttttcaa cagatatttt gcatgtgcca gatcccctcc cgaaacatac caaccataat
      481 aacgatttaa acataaaaca tcgacaatat ctcctacctt acaaacatct ggcgttgatt
      541 ttagataagt aacgattgtt acaggtcttt tctgcggatc aagttcttta gttaattcaa
      601 ctaaaggttc aaaatattct ttagcacctt cggaatccga atctggttca tttgcaatag
      661 accacattac aacacttggg tggttcttgt cgcgttctat caattccttg atcaccgttt
      721 tatgagaatt ttctgttcgt atctctttcc acgtattatg tttttccaca ttatcatcta
      781 ataaagtggc catgaaattt aaatgtaacc ctactgcagg tacttcatca ataactacaa
      841 taccttctcg atctgctaat cgcataattt cttctgagta aggataatgt gatgttctaa
      901 aagaattagc acctatccat ttcattaaat taaaatcttt aatatttaca gcctcattca
      961 aaccacgtcc attaacatac gaatcctcat gcttaccaaa tcctttaaaa taaaaaggtt
     1021 tattattaat caaaaatttg ccattggtaa cctgaacact gcgaatacca aattcttctg
     1081 tataggtatc gatcact
//
LOCUS       DAZKKW010000254         1096 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.264, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000254 DAZKKW010000000
VERSION     DAZKKW010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1096
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.264"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>1096
                     /locus_tag="ACG8PO_003000"
     CDS             <1..>1096
                     /locus_tag="ACG8PO_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFQ0208239.1"
                     /translation="DGEQPPFKGYIVINEGIIEKVENNWNYSDLLNENTELIAYDDQF
                     VMPAIHDNHVFFSGYLAMNAGIDLSDTSSANEAVERIKAVMNHKKADEPIYAHGWDAA
                     LWKTDPSEELLNEINHFGPITAIDKNRSHCWMNAIAKQTYGFTEKETSAESRVLLIKE
                     MLANKKLLAEVYAEFEALLLSKGVISMKEIVFDDAEFLSNIPQRKILSNLYVQAVQEP
                     LQHELLYEYKEKVFPEKVRFGGVKIMVDGVVADRSGDIYGVYQSGVASPEIDYEAIKA
                     EVAAFNEVEIPCCLTTEGDKAGYKAASILVEFGKRLPEGVFNSISDLEMVTQKTAEKM
                     NEGQVVAEVYPQILGLNQTLEEAYMSTVIK"
BASE COUNT          375 a          167 c          247 g          307 t
ORIGIN      
        1 ttgatggaga gcagccacct ttcaaaggtt atattgtaat caatgaaggg atcattgaaa
       61 aggtggaaaa caactggaat tacagtgatc tattaaatga aaacacggaa ttaattgctt
      121 atgatgatca attcgttatg ccagcaattc atgataatca tgtatttttc agtggttatt
      181 tggctatgaa tgctggcata gatttaagcg atacatcttc agctaacgag gctgtagagc
      241 gtattaaagc tgtaatgaac cataaaaaag cagatgaacc aatttatgct catggctggg
      301 atgctgccct gtggaaaact gacccatcag aagagctatt gaatgaaata aaccactttg
      361 gtccaatcac agcgatcgac aaaaatcgca gccattgttg gatgaatgct attgctaagc
      421 agacttatgg ttttacagaa aaagaaacca gtgctgaaag tcgagtatta ttaattaagg
      481 aaatgctagc aaacaaaaag cttttagcag aggtttacgc tgaatttgaa gcattactgc
      541 tttcaaaagg tgttatatca atgaaagaaa tcgtttttga tgacgcagaa tttctttcta
      601 atatacctca gaggaaaata cttagtaacc tatatgtgca ggcagtccaa gaacctctac
      661 agcatgaact tctatatgag tataaggaaa aagttttccc tgaaaaggtc cgctttggtg
      721 gagtgaagat catggtagat ggtgttgttg cggatagaag tggagatatt tacggtgttt
      781 atcagtcagg agtagcttca cccgagattg actatgaggc aattaaagca gaagttgctg
      841 cattcaatga agtagagatt ccttgctgtt tgacaacgga aggagataaa gcgggttata
      901 aagctgcgtc aattcttgtc gaatttggaa agcggttacc tgaaggcgtt tttaattcga
      961 ttagtgattt ggaaatggtt actcaaaaga cagcagaaaa aatgaacgaa ggacaagtag
     1021 tagcagaggt atatccacaa attctcggct tgaatcaaac gttagaagag gcttacatgt
     1081 caactgttat aaaaaa
//
LOCUS       DAZKKW010000255         1085 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.265, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000255 DAZKKW010000000
VERSION     DAZKKW010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1085
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.265"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            83..>1085
                     /locus_tag="ACG8PO_003001"
     CDS             83..>1085
                     /locus_tag="ACG8PO_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HFQ0208240.1"
                     /translation="MSTENQSQKMIIKNWGYKATNVYIEKYYKFPSFLIQNSKYSKMK
                     DSSKIAYMLFKNEFRRALHEKWIDDDGLLYLEFTQADLMDKLNCYQGKVKTILNELET
                     YNLISVVKGEFNPKTKKNEKNKYYLLQPEISADDLFIQNSFDDDLSETIDKSTSSRNA
                     EIARREETAKTLDNTGNAEIARREKTAETLDNTGNAEIAQDYYINKSLDTKRHLKDTE
                     TDQLQNQVLLDNFVEIMKEDSINTFVPEKVLSLIKTFSNTYEEAQQTVTTIHNAKHKA
                     EELEKTVVVYEELESYGIDADKGLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWFIEK
                     "
BASE COUNT          454 a          165 c          173 g          293 t
ORIGIN      
        1 ttattcaccc ttaatacatg gtatagttca cttgttgaaa atcctgaata tatacaagtt
       61 tctaagaaga aggagaaatt taatgtctac agaaaatcaa tctcaaaaaa tgataattaa
      121 aaattgggga tataaagcaa cgaatgttta tattgaaaaa tattataaat ttcctagttt
      181 tttaattcag aattctaaat actccaaaat gaaagacagc tcaaaaatag cctatatgct
      241 ttttaaaaat gaatttcgta gagccttaca tgaaaaatgg atcgacgatg atggtctatt
      301 atatttggaa tttacacaag cagacctaat ggacaaatta aattgctacc aaggcaaagt
      361 aaaaaccatt ttaaacgagt tagaaacata taatttgatt tcagtcgtaa aaggagaatt
      421 taacccaaaa acgaaaaaaa atgaaaaaaa taaatactat ttacttcaac ctgaaataag
      481 tgccgatgat ctttttattc aaaattcttt cgatgacgat ttaagcgaaa ctattgataa
      541 atcaacctct tctcggaatg cggaaatcgc acgtcgagag gaaactgcta aaacccttga
      601 taacactgga aatgcggaaa tcgcacgtcg agaaaaaaca gctgaaaccc ttgataacac
      661 tggaaatgcg gaaatcgcac aagattatta tattaataaa tctttagaca ctaaaagaca
      721 cttaaaagac actgaaacag accaattaca aaatcaagtt ttgctagata acttcgttga
      781 aatcatgaaa gaagacagca taaacacatt tgtaccagaa aaggtattaa gcttgataaa
      841 aacattttct aacacttacg aagaagccca acaaaccgtc accacaatac acaacgcaaa
      901 acataaagcc gaggaacttg agaagacggt agttgtgtat gaagaacttg aatcttacgg
      961 aattgatgcg gataaaggat tatatatgac gttgctgaaa gcttatcaaa aacaaaagac
     1021 tgagaaagta aataatttgc agaatttgat ttttgtgtat gtaaaaaact ggtttattga
     1081 aaaag
//
LOCUS       DAZKKW010000256         1064 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.266, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000256 DAZKKW010000000
VERSION     DAZKKW010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1064
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.266"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(39..>1064)
                     /locus_tag="ACG8PO_003002"
     CDS             complement(39..>1064)
                     /locus_tag="ACG8PO_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HFQ0208241.1"
                     /translation="LTGNPKNIRLFLYNLFNSTEISFVELYTLPIIIKTEKMLKDLKQ
                     YYPLSAYEKKKLFHFLAINLLAEQADSLDIASNQSLFFSDIGFYHLKGVLGEKSVIFI
                     NKTLSWLYLHDKLDQKYLRKNEDETIEWLNERFIESFENQFNLLREETSQLIRRELTK
                     IHFKLLHYTINKADEFPLDVSFFRENYPAFYCYLIEYINDLTSKRKELLQDKFFLFFS
                     YLLLLINHIPIQLISEPVKIIIDFSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFPD
                     VIITNRNNLYEESASQVVVWLDPPRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPE
                     EIIDRIE"
BASE COUNT          346 a          177 c          160 g          381 t
ORIGIN      
        1 ttttagaaaa agcagtcgga ctttctaaaa gaagttcact attcgatcct gtcgatgatt
       61 tcttcaggaa aatcgtctat ttgcttgtca attttgctgt ctttgtattt ctcttcttgg
      121 atagtcaaca aaatctttgt aagattcccc cagtctactg ctcttggcgg atcgagccaa
      181 accactactt gagatgcact ttcttcatat agattattcc tattcgtgat tataacatcc
      241 gggaactcag gctttcctgc ctctatcact tccgtgttca gattaatgta caagcttaaa
      301 tttttcttga taaattgatt ataggcagca ccataagaaa aatcgatgat gatctttact
      361 ggttcagaaa tcagttgaat gggtatgtga ttgatcaata ataataaata actgaaaaaa
      421 agaaaaaatt tatcttgaag caactctttg cgttttgacg ttagatcatt tatatactct
      481 atcaaatagc aataaaatgc tggatagttt tctctaaaaa aagaaacatc aagtggaaat
      541 tcatcagctt tattaatagt gtaatgcaaa agtttaaaat gtattttggt taactctctt
      601 cggatcaatt ggctcgtctc ttctcgcaac agattaaact gattttcaaa actttcaatg
      661 aatcgttcat tcaaccattc gattgtttca tcttcatttt ttctcaagta tttttgatcc
      721 aatttatcat gaagataaag ccaagagagt gtcttattga taaaaataac ggatttttct
      781 cctaatactc cttttaagtg gtaaaatcct atatccgaaa aaaatagcga ctgattgcta
      841 gcaatatcta aagaatcagc ttgttcagcc aacaggttga ttgctaaaaa gtggaataat
      901 ttcttttttt cataagcaga cagaggataa tactgtttta aatcttttaa cattttttct
      961 gtttttataa ttataggtag cgtatacaat tccacaaaac ttatctctgt tgagttaaaa
     1021 agattatata aaaaaagccg aatgttttta ggattccctg tcaa
//
LOCUS       DAZKKW010000257         1035 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.267, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000257 DAZKKW010000000
VERSION     DAZKKW010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1035
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.267"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>1035
                     /locus_tag="ACG8PO_003003"
     rRNA            <1..>1035
                     /locus_tag="ACG8PO_003003"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          316 a          208 c          298 g          213 t
ORIGIN      
        1 gaactgaaac atctaagtac ctgcaggaag agaaagaaaa ttcgattccc tgagtagcgg
       61 cgagcgaaac gggaaaagcc caaaccagca agcttgcttg ttggggttgt aggactccaa
      121 tatggtagtt ctttcagata gtcgaatgac ttggaaaagt cagtcaaaga gggtaaaaac
      181 cccgtagacg aaatgtggaa gacacctagg aggatcctga gtacggcgga acacgagaaa
      241 ttccgtcgga atccgggagg accatctccc aaggctaaat actccctagt gaccgatagt
      301 gaaccagtac cgtgagggaa aggtgaaaag caccccggaa ggggagtgaa atagaacctg
      361 aaaccgtgtg cctacaacaa gtcaaagccc gttaatgggt gatggcgtgc cttttgtaga
      421 atgaaccggc gagttacgat tgcatgcgag gttaagttga agagacggag ccgcagcgaa
      481 agcgagtctg aatagggcgt ttgagtatgt agtcgtagac ccgaaaccat gtgatctacc
      541 catgtccagg ttgaaggtgc ggtaaaacgc actggaggac cgaacccacg tacgttgaaa
      601 agtgcgggga tgaggtgtgg gtagcggaga aattccaaac gaacttggag atagctggtt
      661 ctctccgaaa tagctttagg gctagcctcg gaattgagaa tgatggaggt agagcactgt
      721 ttggactagg ggcccatctc gggttaccga attcagataa actccgaatg ccattcattc
      781 atatccggga gtcagactgt gagtgataag atccatagtc gaaagggaaa cagcccagac
      841 caccagctaa ggtcccaaaa tatatgttaa gtggaaaagg atgtggggtt gcacagacaa
      901 ctaggatgtt ggcttagaag cagccaccat ttaaagagtg cgtaatagct cactagtcga
      961 gtgaccctgc gccgaaaatg taccggggct aaacatatta ccgaagctgt ggagtacacc
     1021 tttaggtgta ttggt
//
LOCUS       DAZKKW010000258         1012 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.268, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000258 DAZKKW010000000
VERSION     DAZKKW010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1012
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.268"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..101)
                     /locus_tag="ACG8PO_003004"
     CDS             complement(<1..101)
                     /locus_tag="ACG8PO_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
                     /protein_id="HFQ0208242.1"
                     /translation="MKNYDPNSRWGTHTIKVSFQRWDYKGFVTFRRGG"
     gene            complement(88..417)
                     /locus_tag="ACG8PO_003005"
     CDS             complement(88..417)
                     /locus_tag="ACG8PO_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0208243.1"
                     /translation="MIQKFAKMSKTAWIEKLENDLSVMRLAPKKFKREIAEKEAILSV
                     LNAVDDKCPVKISFSDIATIIEGIYYGEVDEEISSTKDLLDFLDKNKDTFIVLTDYEE
                     VTTNEKL"
BASE COUNT          304 a          230 c          129 g          349 t
ORIGIN      
        1 ccgcctcttc taaacgtcac aaatcctttg taatcccaac gttggaaaga aaccttaatc
       61 gtatgtgttc cccatcggct attcggatca taatttttca tttgttgtaa cctcctcata
      121 atcagttagt actataaagg tatctttgtt tttatccaaa aaatctaata aatcttttgt
      181 agaagatatc tcttcatcaa cttcaccata ataaatacct tcgataattg ttgctatatc
      241 tgaaaaacta atttttactg gacatttatc gtctaccgca ttcaataccg acaaaattgc
      301 ttctttttct gcaatctctc gtttgaattt ctttggagct aatcgcatga cactcaagtc
      361 attttcaagc ttttcaatcc atgcagtttt actcattttc gcaaattttt gaatcattcc
      421 tctaccacct ttgctatcta ctaaattagt tttacttcat aacgaatatt tttatcgttt
      481 ttcgttcact caaaaatata taaaataaat cgactcgttt atggcgtccg aaacgagaaa
      541 ccctagtaga aaacggtaaa tgctcgattg atccaaagat caacgtcttc gcaagtatct
      601 acatgtactg cagcacacga cacaatttct tgtgcgtcct ccccattacg agaagaccta
      661 ctccttattc catgagtttg cgtcaaagcc tagatccctt agtagctgct catgattaat
      721 ctaggcgcta ttctaacttg tctaacttct aaattccctc ttgtttactc tgtgcatatt
      781 atttacacaa attcatttca ccactatcca aacaagggca tttactcgat tgaccaattt
      841 tttacgttac caatcgtaaa aacctacccc gtcacccccc gaaaggaatt gttgatagat
      901 caagtctact ccttattttt ctagtgattc taagttcctt tagaagtctc gacaatattt
      961 tcaagcaagc gaaaaacgat aaaatacaag ttatgagaat gatcaattca gc
//
LOCUS       DAZKKW010000259         1007 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.269, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000259 DAZKKW010000000
VERSION     DAZKKW010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1007
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.269"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            119..>1007
                     /locus_tag="ACG8PO_003006"
     CDS             119..>1007
                     /locus_tag="ACG8PO_003006"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0208244.1"
                     /translation="MDFFIYTKYFTLSMKQHKAEQKDNKLEIKKMKELLGIGFVKPNS
                     KVTIASLATTNNNGKIVNGFDDSDIDILSKSYFSLYGDFADLMGIDSYLVNNFEIKGS
                     ELNDIVYFVLISLYAIDQNTEYRQIDYLLVFYSLLYQFVLTRLFKNAKEELILNSDYE
                     IAKQIASIRKENKSEVIKLRKELSIASKENGRLESLVKSAKKEEELLKQEIKELKGIR
                     KELEQLRFENKVLKQLQEQEHRQSLNGKTEEPSLSEMIAYLKHKNILLVGGHVNFQNK
                     LTELLPNIKMFDVSENNGKI"
BASE COUNT          386 a          124 c          180 g          317 t
ORIGIN      
        1 cgcctttagt gactagagtt tgcacaattt ctgtagtaaa atcattcata gggaagtcct
       61 cttttctgtg aattggttgt cgttaacttt attctacaga agcgacttcc ttttttgtat
      121 ggattttttc atttacacaa aatattttac actctctatg aaacagcata aagccgaaca
      181 aaaggataac aaacttgaaa ttaaaaagat gaaagaacta ttaggtatag gatttgtaaa
      241 gcccaattca aaagtcacta ttgcttcgtt agcaactact aacaataatg ggaaaatagt
      301 aaatgggttt gacgattcag atatagatat attgtcaaaa tcatattttt ctttgtatgg
      361 agattttgca gatttgatgg gtattgatag ttacttagtc aataattttg agatcaaggg
      421 tagcgagctg aatgatattg tgtatttcgt tttaatctct ctatatgcaa ttgatcagaa
      481 taccgagtac cgacaaattg actatttgct agttttttat agtttgttat atcagttcgt
      541 tttaactcgg ttgtttaaaa atgctaagga agaattgatc ctgaattctg attatgaaat
      601 agcaaaacaa attgcaagta ttagaaaaga aaacaaatct gaagtaatca aattaagaaa
      661 agaattaagt attgcgagta aagaaaacgg tcggttagaa tctttagtca aatcggcaaa
      721 aaaagaagag gaacttttga agcaagagat taaagaatta aaaggaatca gaaaagaact
      781 tgaacagctg aggtttgaaa ataaagtatt aaagcaattg caggagcaag aacatagaca
      841 atcattaaat ggtaaaactg aagagccttc actgtctgaa atgatagctt atctaaaaca
      901 taaaaacatt cttttagtgg gaggacatgt aaattttcaa aataaattaa ctgaactatt
      961 accaaatatt aaaatgtttg atgtaagcga aaataatgga aagatac
//
LOCUS       DAZKKW010000260         1001 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.270, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000260 DAZKKW010000000
VERSION     DAZKKW010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1001
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.270"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(81..635)
                     /locus_tag="ACG8PO_003007"
     CDS             complement(81..635)
                     /locus_tag="ACG8PO_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0208245.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADVAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGG"
BASE COUNT          324 a          186 c          107 g          384 t
ORIGIN      
        1 cttccgaaaa ttcagttatc ttcattaatc tcaccttcta agttttcatt atacacaaaa
       61 aatatatcct aatatctcaa ttagcctccc aatttaaaac cacgtaacga tgcccctatt
      121 tcatcttcag ttataccaat gtaccttttt gtaattgctt gcgagctatg attaaaaata
      181 aacattaagg tagcaatgtc tctagttttc ttgtagtagt gatacccaaa agtttttctt
      241 aatgtatgtg ttcctatatc atctcttcct aatttttctg ctacatctgc atatatctga
      301 tagacacgat cccttgttat atgtcgacct tgttgccgac ttggaaataa ccaatcattt
      361 tcttctttac cttcagtata taataaaatc tcttctctca aattatctat aaaaatcggt
      421 tttcgcttac ctgttttatg ttcaacaata ataggcttac ttttacctcg tatgtctcca
      481 acttttaatc ctataatatc tgacatacgt aacccattat tgattccaaa caaaaataaa
      541 aaactatctc tttctggatt acttgttctt cttagccaaa actgaaaatc ttgtatctct
      601 tcatttgtcc ttaacggttg tacattatag ctcactattg tttcccttct taaaaatatt
      661 ctgagttcat gattatatat atattttacc ataatcatga acttatatat atactctgtt
      721 aaaagtgaag aaaatcaagg ggtttagttt atgattttat atcatttcag taactcagaa
      781 tattccgact tttttgtaca aaaaaattaa ccttacttat atgaagattc taagctctta
      841 tttaaatttt agacgggcag ttatccgtag aagttcttag aatagctaat ttacataatt
      901 aaattatatg aaatttaagt ttgaacaata tactaagaac tccccttact ataaagtgaa
      961 ataaattagc atatgaataa ccgtgctacc aaacttttca t
//
LOCUS       DAZKKW010000261         1000 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.271, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000261 DAZKKW010000000
VERSION     DAZKKW010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1000
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.271"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            369..1000
                     /locus_tag="ACG8PO_003008"
                     /pseudo
     CDS             369..1000
                     /locus_tag="ACG8PO_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          342 a          181 c          183 g          294 t
ORIGIN      
        1 tttcaaagtt agtagagaaa atgtagttat atatttaaat ttttaccaga aagaaatgaa
       61 caccttcaat tatctaaatc tcgaaaatgg ttagatgtaa tcatcaggaa aaacaatctt
      121 aaacgaatta ctattcatgg tcttagacac acacatgcta gcttgctttt agcaagctag
      181 tgcaaatatt aaagatgtgc aagagcgttt aggccactct tctattcaaa tcattatgga
      241 tctatatatt catattacag acaaaagaaa agaagaaacc gtagcgcaat tcgcaaaata
      301 taggctctat aatttatggg actgatgaat caaaattgat tcttcagtcc cattgttatt
      361 ttaggtatat gattcgtaca ttgaaagagt tgaaaaacta tcttccgaag ccgtcaaaaa
      421 ggaccctgcc aaaagtattg atggtggatg aatttcgttc gcacgcttca attgtggata
      481 aaatgagctt tatttgtgcg gatggcgaga ctggaaaact cattgatatt ttgcctacac
      541 gaaaattgtc tcgattaaca cactatttta gtcgctgtgc aaatcctgaa gcggtggaat
      601 atttagtgac agatatgaac gccgcctact tccaactgac aaaacgtgtc ttaccaaatg
      661 cgaaagtcgt cattgattgt tttcatatca tcaaacattt gaatcaggcg ttcaatgatt
      721 ttcgtgtaca agaaatgaaa agattacacc agttagggca acgacgggaa gccgagaaag
      781 tgaaaaagaa ctggcgcttt cttctaaaaa accgtcaaaa aatcaatcac tatgattaca
      841 aaacatggcg cagttttcgt gcacctaaat acccgctact cactgaagca atgatgattg
      901 atcgattgct ggcattttca acgcctttaa aggacgccta tacttttttc catgagctga
      961 ccgaagcctt tagagacaag gaatcagact tattttttac
//
LOCUS       DAZKKW010000262          997 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.272, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000262 DAZKKW010000000
VERSION     DAZKKW010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..997
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.272"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..756
                     /locus_tag="ACG8PO_003009"
     CDS             <1..756
                     /locus_tag="ACG8PO_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFQ0208246.1"
                     /translation="ENQGYLKSVGTNGWQLFDQYGEDIAYIEMLKFHPDKGRIDFNPN
                     KIGEFLKGSMKQFIHDLFLDAHFSRADIACDILGVPDDFITQYRIVDPVSFKPIYGRS
                     GKLETAYWGSRASERQVRMYNKKLEQERKKQIVPKEIETWWRIEMQLRRGKATDWHAM
                     VRESLDSFASPHYLPGDVKPVDRIMIKGLNQDHSEWAYISRNLKYRLRKLLKEESQND
                     ELTNHLRETFKESANDLKIELDTWLLGLDVTEK"
BASE COUNT          327 a          153 c          240 g          277 t
ORIGIN      
        1 gaaaatcaag ggtatttgaa gtcggttggt acaaatggtt ggcaattatt tgaccaatat
       61 ggagaagata tcgcctatat cgaaatgtta aaatttcacc ctgacaaagg tcgtattgat
      121 tttaatccaa ataaaattgg cgagttttta aaggggtcga tgaaacaatt cattcatgat
      181 ttgtttctgg atgcgcattt ctcacgtgca gatattgctt gcgatattct cggtgttcct
      241 gatgatttta ttacccagta tcgaatcgtt gaccctgtgt cttttaagcc aatttatggc
      301 cgttctggca aattagaaac agcgtactgg ggtagtcgtg ccagcgagcg acaagtgcgg
      361 atgtacaaca aaaagcttga acaggaacga aaaaaacaga ttgtaccaaa agaaatcgaa
      421 acatggtggc gtatagaaat gcagctaaga cgtggcaaag caacagattg gcatgcgatg
      481 gttcgggaaa gtttagatag ctttgcaagt cctcattact tgcctggaga tgtaaaacct
      541 gttgatagaa ttatgataaa agggctgaat caagatcatt cagagtgggc gtatattagc
      601 agaaatttga aatatagatt gcgcaaattg ttgaaagaag aaagccaaaa cgatgagttg
      661 acgaatcatt tacgagagac gtttaaggaa tctgcaaacg acttgaaaat agaattagac
      721 acgtggcttt tgggtttgga cgtgacagag aagtgatacc gaataagaaa agtcagatga
      781 gtgagagtaa atttacgtca atgtttaatg acgtgagttt acgataagcg attattttgc
      841 gtgaagcaaa ataaagcgta aaaggagttg agagcgtgac acgctttcgg ctcctttttt
      901 cgttgtcact tgtgacagcg aataccctcg gagagcattt ggaattgtga cagccatgtc
      961 acaattagga aaatgttact ctttgtcaaa gagtaac
//
LOCUS       DAZKKW010000263          994 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.273, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000263 DAZKKW010000000
VERSION     DAZKKW010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..994
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.273"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>994)
                     /locus_tag="ACG8PO_003010"
     rRNA            complement(<1..>994)
                     /locus_tag="ACG8PO_003010"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          209 a          307 c          225 g          253 t
ORIGIN      
        1 agcacgtgtg tagcccaggt cataaggggc atgatgattt gacgtcatcc ccaccttcct
       61 ccggtttgtc accggcagtc ttgctagagt gcccaactga atgatggcaa ctaacaataa
      121 gggttgcgct cgttgcggga cttaacccaa catctcacga cacgagctga cgacaaccat
      181 gcaccacctg tcactttgcc cccgaagggg aagctctatc tctagagtgg tcaaaggatg
      241 tcaagacctg gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc caccgcttgt
      301 gcgggccccc gtcaattcct ttgagtttca accttgcggt cgtactcccc aggcggagtg
      361 cttaatgcgt tagctgcagc actgaagggc ggaaaccctc caacacttag cactcatcgt
      421 ttacggcgtg gactaccagg gtatctaatc ctgtttgctc cccacgcttt cgagcctcag
      481 cgtcagttac agaccagaga gccgccttcg ccactggtgt tcctccatat atctacgcat
      541 ttcaccgcta cacatggaat tccactctcc tcttctgcac tcaagtctcc cagtttccaa
      601 tgaccctccc cggttgagcc gggggctttc acatcagact taagaaaccg cctgcgctcg
      661 ctttacgccc aataaatccg gacaacgctt gccacctacg tattaccgcg gctgctggca
      721 cgtagttagc cgtggctttc tggttagata ccgtcaaggg atgaacagtt actctcatcc
      781 ttgttcttct ctaacaacag agttttacga tccgaaaacc ttcttcactc acgcggcgtt
      841 gctcggtcag actttcgtcc attgccgaag attccctact gctgcctccc gtaggagttt
      901 gggccgtgtc tcagtcccaa tgtggccgat caccctctca ggtcggctat gcatcgtggc
      961 cttggtgagc cgttacctca ccaactagct aatg
//
LOCUS       DAZKKW010000264          988 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.274, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000264 DAZKKW010000000
VERSION     DAZKKW010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..988
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.274"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..328
                     /locus_tag="ACG8PO_003011"
     CDS             <1..328
                     /locus_tag="ACG8PO_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFQ0208247.1"
                     /translation="HALSAFRDEDESILVQIKKLLATIDIEETYILTDILGGSVNNDM
                     LTIIKEYEGLQLITGMNLPLVISIATYNGKIGESELEKLIKESQQSVIDCRKLLVEQL
                     TGGDDL"
     gene            325..795
                     /locus_tag="ACG8PO_003012"
     CDS             325..795
                     /locus_tag="ACG8PO_003012"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0208248.1"
                     /translation="MIKLVRIDHRLLHGQVVFSWSKSLNINRIIIANKEAANDEFKKM
                     SLNLSKPTGIKLTIFSVEQLLEKMPKIETLNENIMLIFGNTKEIRQFCEAYPKVQEIN
                     YGGIIKKEGSKQFSNAIFLNESEQEDAKKLKALGIKQFIQQVPTSKKEDLNTML"
     gene            809..>988
                     /locus_tag="ACG8PO_003013"
     CDS             809..>988
                     /locus_tag="ACG8PO_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFQ0208249.1"
                     /translation="MLVQSIGLGLIGVFCILDSRLLGRMNFERPLIVSTLVGLVLGDM
                     EKGLIVGASLELMSLG"
BASE COUNT          360 a          125 c          190 g          313 t
ORIGIN      
        1 tcatgccttg tcagcattta gagatgaaga tgaatctatt ttggtacaaa taaaaaaact
       61 tttagccaca atagatatag aagaaaccta cattttaact gatattctag gtggtagtgt
      121 gaataatgat atgctcacta ttattaaaga gtatgaggga ttgcaattaa ttactggaat
      181 gaacttacct ttagttattt ctattgcaac ctataatgga aaaattgggg aaagtgagct
      241 agaaaagctt attaaagaga gtcaacaatc agtgattgat tgtagaaaat tattagtaga
      301 acaacttact ggaggagatg acctatgatt aagttagtac gtattgacca tcgtttgttg
      361 cacggacagg tagtattttc ttggagtaaa tcattaaata tcaatcgtat tattattgcc
      421 aataaagagg cagcgaatga tgaattcaaa aaaatgtctt tgaatttatc taaaccgact
      481 ggcattaaat taacaatttt ttcagttgaa cagttactag aaaaaatgcc taaaatcgaa
      541 acacttaacg aaaatatcat gcttattttt ggtaatacaa aagaaattcg tcaattttgt
      601 gaagcctatc ctaaagtaca agaaatcaac tatggaggga taatcaaaaa agaaggttcc
      661 aagcaattta gtaatgccat tttcttgaat gaatctgagc aagaagatgc caaaaaatta
      721 aaagctcttg gcattaaaca atttattcaa caggtaccaa cttctaaaaa agaagatttg
      781 aatacaatgt tataaaggag gaatgaaaat gctcgttcag tcaattggtt tagggttaat
      841 cggtgttttt tgtattttag actcaaggct tttaggaaga atgaattttg aacgaccgtt
      901 gattgtcagt acgcttgttg gtttggtact tggagacatg gaaaaaggat taattgttgg
      961 agcctctttg gaattgatgt ccttagga
//
LOCUS       DAZKKW010000265          949 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.275, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000265 DAZKKW010000000
VERSION     DAZKKW010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..949
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.275"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            55..>949
                     /locus_tag="ACG8PO_003014"
     CDS             55..>949
                     /locus_tag="ACG8PO_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFQ0208250.1"
                     /translation="MDVAKTVNAIVEKNDGEISGIYLVACGGSLVDMYVSDYFLKAEA
                     SKCITGLYTANEFNHVTPKQLDDKSVVILCSHGGNTAETVKAGEVAQARGAVTVGLTH
                     NKTADLLKVSDHSFLYEWGDDAKIVNNPMAIILDLTVSLVNVTETFEHYGKFREGMKV
                     IDTVIENGIKQVQDRIKVFADKYKDETMFYILGSGPSYGHAYGFSICSLMEMQWLDST
                     SVHSGEFFHGPFEVTDKDTNFILLMSIGRTRALDERAKRFLDKYAERVEIVDAKELGL
                     DMISDEVSEFFNPILFYSILSE"
BASE COUNT          306 a          135 c          202 g          306 t
ORIGIN      
        1 tacttttatt ttggataaaa ttaacatttt taaaatttta aggaggaaat gttgatggat
       61 gtagcaaaaa cggttaacgc aattgtagaa aaaaatgatg gagagatttc tggaatttat
      121 ttggtggctt gtggcggttc gctggtagat atgtatgtct cagattattt tcttaaagcc
      181 gaagcatcga agtgtattac aggcttatat acagcgaacg aatttaacca tgtaacccca
      241 aagcaattag atgataaatc tgttgtcatt ctatgttctc atggaggaaa tacagcagaa
      301 acggtaaaag caggagaagt cgctcaagct agaggagctg tgactgttgg tctgactcat
      361 aataaaactg cggatttatt aaaggtttct gatcatagtt tcctttacga atggggagat
      421 gatgcaaaaa tagtcaataa tcctatggcc atcattcttg atttaactgt atctttggtc
      481 aatgttaccg aaacatttga acattacggt aaattcagag aaggcatgaa ggttatcgat
      541 accgtaattg aaaatggcat taagcaggtg caggatcgta ttaaagtgtt tgctgataag
      601 tacaaagatg aaacaatgtt ttatatctta ggtagtggac catcatatgg acatgcttat
      661 ggtttttcaa tttgctcttt gatggaaatg caatggcttg attcaacttc tgttcattct
      721 ggagaattct tccatggacc ttttgaagta acagacaaag atacgaattt tattctcttg
      781 atgagcattg gacgcactcg tgctttagat gaacgggcaa aacgattcct tgataaatat
      841 gcagaaagag tagaaatcgt tgatgcaaag gaactaggat tggatatgat atcagatgaa
      901 gtttctgaat tcttcaatcc cattttattc tacagtattc tttcagaat
//
LOCUS       DAZKKW010000266          946 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.276, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000266 DAZKKW010000000
VERSION     DAZKKW010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..946
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.276"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            733..>946
                     /locus_tag="ACG8PO_003015"
     CDS             733..>946
                     /locus_tag="ACG8PO_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFQ0208251.1"
                     /translation="MSKKGIIVNEWEKLNRISNRRLSVDDKVHPLKLSWSIDRITIVG
                     KLNDTIAYWTQNGVVFVDFEQLMRLNE"
BASE COUNT          309 a          142 c          215 g          280 t
ORIGIN      
        1 caaagagtaa cagaaaggtt tacactcttt ttattagcga cagcgcgaag gaggggttca
       61 ccttagggtt tagtatttcg ttcaaaaagg gaacaaagaa tacaagcatt tctcataata
      121 atcgtgagtt aactgatagc caaaaaatca atggtttgtc tggtagaaaa taattagcag
      181 atttacaagt gggaaataaa aagctgaaag aagaaaattg catttcaaac tacattttct
      241 acaataactc taaaatggca tattttgcgt tctgagcggt tttagcgttt atttcgttta
      301 gttatcggca caatcggtga aacacgttag aggagcgaaa atggagcctg taaggcgact
      361 ggtagcggaa cggtcgtgtt agattgtgta gccgatagct gaacgaaata aatgctggca
      421 gcgccattct atttcggcag gaggaggctc aaggggttcg gggaatgaaa ttccctgagg
      481 tctttttatt ttttctagag gagtgctata atcaaagaaa atgagaaaaa agagtaaaaa
      541 aatagtcgag cagtgttgac gcactctcga cctttacgga aactacttca cctagttccg
      601 taactcaagt tcattatacg aatattttta ggtttcttca agctagagat ttttggatat
      661 gaatttttag ggttacgtga actctggaaa ttctttccaa gaatctcttt tttcgttgag
      721 gaggagctat ttttgagtaa aaaaggcata atcgtaaatg agtgggaaaa acttaaccgt
      781 atttctaaca ggcggttaag tgttgacgat aaggtacacc ccctaaaatt aagttggtca
      841 atcgatcgaa ttacaatcgt cggaaaactg aatgatacca tagcttattg gacgcaaaat
      901 ggagttgtgt ttgtcgattt tgagcaatta atgcgtttga atgaaa
//
LOCUS       DAZKKW010000267          943 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.277, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000267 DAZKKW010000000
VERSION     DAZKKW010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..943
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.277"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            159..383
                     /locus_tag="ACG8PO_003016"
     CDS             159..383
                     /locus_tag="ACG8PO_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HFQ0208252.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            403..690
                     /locus_tag="ACG8PO_003017"
     CDS             403..690
                     /locus_tag="ACG8PO_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFQ0208253.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
BASE COUNT          370 a           94 c          174 g          305 t
ORIGIN      
        1 taaataactg atcttataat tttatttaaa cgattatgaa ttcaaaacta ctttttatga
       61 cgttaatcat tgttatataa taaaatgtta tataataaaa ttactaagat tatttaatat
      121 aacaagcaat tttaaaaaaa cgaaaaggaa aaacaatcat gaaaaagaaa gtattaaaac
      181 attgtgttat tctaggaata ttaggaactt gtctagctgg catcggtaca ggaataaaag
      241 ttgatgcagc tacttactat ggaaatggtc tttattgtaa caaagaaaaa tgttgggtag
      301 attggaatca agctaaagga gaaattggaa aaattattgt taatggttgg gttaatcatg
      361 gtccatgggc acctagaagg tagtggtagg gaggttatta taatggattt tactaaagaa
      421 gaaaaacttt taaatgcaat tagtaaagta tacaatgaag caactataga tgactatcct
      481 gacttaaaag aaaagctctt tctttattct aaagaaatca gtgagggaaa aagtgttggt
      541 gaagttagta tgaaattaag tagttttctt ggaagatata ttttaaaaca taaatttgga
      601 ttacctaaat ctttaataga attacaagaa attgttagta aggaatctca agtatataga
      661 ggatgggctt ctattggtat ttggagttaa ttctttggaa taaaatgaaa atgggactga
      721 agaatcaatt ctgattcatc agtcccataa attatagagc cgaaaaatta tcatatttat
      781 agatttatta atagtaaatg gcataagaat ctttgaacaa cgaaaaaaat gtagctctac
      841 ctaattattg atttgcaaca atttcaccga aggtgaaaca agcgaaactt tacaaaaagt
      901 ggctagcatt tgctagtctt ttttgtattg tggagagtgg ggg
//
LOCUS       DAZKKW010000268          937 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.278, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000268 DAZKKW010000000
VERSION     DAZKKW010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..937
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.278"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..818
                     /locus_tag="ACG8PO_003018"
     CDS             <1..818
                     /locus_tag="ACG8PO_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFQ0208254.1"
                     /translation="TRYQLIKQLIRTKQHFLENLTYKCNTLAREMRDESTSLFSATLI
                     SLMTEDFTLDELAELPLEAFCDLLQEKGKGRFKQPEKIAKAIQRAITMSYRLGALAQE
                     SINVVLSVLVREIRALEKNIKELDKAIEQVIVVIPEYQCLTSIPGVGKVYAAGLIAEI
                     GQIERFEDQTKLAKYAGLSWKVNQSGNYQSQNTPLTKQGNRYFRYYLVEAANSVKNYL
                     PEYKAFYQSKYKEVPKHQHKRALVLTARKFTRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT          334 a          182 c          164 g          257 t
ORIGIN      
        1 gtactcgcta tcaacttatt aagcaattga ttcgtacaaa gcaacatttt cttgaaaatc
       61 taacgtacaa atgtaatacg cttgctcgag aaatgcgaga cgaatcgacg agtcttttta
      121 gtgcaaccct catttcactc atgacggaag atttcacatt ggatgaactt gctgagcttc
      181 ctttagaagc tttctgtgat ttacttcaag aaaaaggaaa aggccgtttc aagcaaccag
      241 aaaaaatcgc taaagcaatt caacgtgcaa ttaccatgag ttaccgccta ggtgctcttg
      301 cacaagaatc aattaatgtt gttttaagtg ttctcgtacg agaaatacga gcacttgaaa
      361 aaaatatcaa agaattagat aaagccatcg aacaagtaat tgttgtcatt ccagaatatc
      421 aatgcttaac cagtattcca ggcgttggta aagtctatgc tgccggttta atcgctgaaa
      481 ttggtcaaat cgaaagattt gaagatcaga caaaattggc aaaatacgct ggattaagtt
      541 ggaaagtgaa ccaatctgga aactatcagt cgcaaaatac acctctgaca aaacaaggaa
      601 atcgatattt tagatactac ttagttgaag ctgccaactc tgtaaaaaat tatcttcctg
      661 aatacaaagc tttttatcaa agtaaatata aggaggttcc aaagcatcaa cacaaacgtg
      721 cactcgtcct aaccgcaaga aaatttacgc gtctggtgga tacgctacta cgtaaacacc
      781 aactctatac gccaccaagg agtgtgatag agaaataaca atccgttatt gacgcaaatc
      841 cttgaatcaa cccgaaaaaa atttgatttt gatcgggtct agtttcgtgc gcttcaaaac
      901 atattactca ataaagaaaa tcttaaattt catcttg
//
LOCUS       DAZKKW010000269          931 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.279, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000269 DAZKKW010000000
VERSION     DAZKKW010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..931
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.279"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..142)
                     /locus_tag="ACG8PO_003019"
     CDS             complement(<1..142)
                     /locus_tag="ACG8PO_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFQ0208255.1"
                     /translation="MSKRTRRTFSQEFKQQIVNLYLAGKPRVEIIREYELTASAFDKW
                     VKQ"
     gene            complement(183..497)
                     /locus_tag="ACG8PO_003020"
     CDS             complement(183..497)
                     /locus_tag="ACG8PO_003020"
                     /inference="COORDINATES: protein motif:HMM:NF024673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFQ0208256.1"
                     /translation="MLCRLLEIPRSVYYFYKNKPLTATEIRNNKLKKKISTIFFTNKQ
                     RYGATKIHQVLLKEGISVSLKHVQKLMKQLNLRSIVVKKYRPQRSNKPIMDRLQLTRQ
                     KN"
     gene            complement(548..802)
                     /locus_tag="ACG8PO_003021"
     CDS             complement(548..802)
                     /locus_tag="ACG8PO_003021"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFQ0208257.1"
                     /translation="MTRYEENFKQMIVELNQTGRSVRGLAKEYGLSEATIYKWKNLYL
                     PDQSTGLTGKEVAELRKENARLNEELEILKKAAAIFSRKT"
BASE COUNT          270 a          170 c          135 g          356 t
ORIGIN      
        1 attgctttac ccatttgtca aatgctgaag ccgttagttc atattctcga atgatttcta
       61 cacgtggctt tccagctaag taaagattga cgatttgttg cttgaattct tgtgaaaaag
      121 ttcttcgtgt tctcttagac ataaaaattc ctcctggtat gttttcttct agtctacaca
      181 ccttaatttt tctgtctagt taattgtagc ctatccatga tcggtttatt agacctttga
      241 ggtctatatt ttttaactac aatcgacctt aaatttaatt gtttcattag cttttggaca
      301 tgtttaagag atactgaaat cccttctttt aataaaactt gatggatctt tgtggcacca
      361 tagcgttgtt tattcgtaaa aaaaattgtt gaaatcttct ttttcaactt attatttctg
      421 atttctgtag ctgtcaacgg tttattctta taaaaataat agacacttct agggatttct
      481 aataaccgac acaacaaaga aacagtgtaa tccttacacc atttttcaat aaattgaaca
      541 agcgaattta ggttttccga gagaatatgg ctgcggcttt ttttaagatt tcaagttcct
      601 catttaaacg agcattttct tttctcagtt cagctacttc ttttccagtc agtcctgtgg
      661 actgatcagg taaatataaa ttcttccatt tgtaaatcgt tgcttcagat aagccatatt
      721 ctttcgctaa ccctcgaaca gaacgtccag tttgattcag ttcaacaatc atttgtttaa
      781 aattttcttc atatcgggtc ataaaaattc atcctctcgt agataagttt aatttattct
      841 tgtctctacg aaaagctgtc taagatttga tactaacacc aaatgttgta tacccccaaa
      901 caatacacat attttgaagg attaggatta a
//
LOCUS       DAZKKW010000270          931 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.280, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000270 DAZKKW010000000
VERSION     DAZKKW010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..931
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.280"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            47..484
                     /locus_tag="ACG8PO_003022"
     CDS             47..484
                     /locus_tag="ACG8PO_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0208258.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKERLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNAKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYGKFVETKKAKTVSSEWLDYFENINKYGAYYTMKKEDNENE"
     gene            477..>931
                     /locus_tag="ACG8PO_003023"
     CDS             477..>931
                     /locus_tag="ACG8PO_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0208259.1"
                     /translation="MNKVIYQLTTEDVSHFLTNKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYLESETSQTKVTVSNNYLDVYKHRPETVKKGTELLRLETEKYIMNVYLERS
                     ELVENFVSCTICRKHYYSESEYYSIFESMELMEEEMEELNSKNATIDDY"
BASE COUNT          381 a          133 c          186 g          231 t
ORIGIN      
        1 cgaatattgg ttaaccaaat tagagaacta aaaaggagaa aaaaatatgg ctcaaaatta
       61 ctatgacgaa tttgtcaaat tacctttgga taaaatggca caaaaaatgg aggatatgac
      121 attcctatac catgagacac gtgttccaaa aaaacattat aaggaaagat tgtcggtagc
      181 tgtggaagaa atgattgaat caggtgtcga aatgaatctc attgcgacgt actatcgaac
      241 actggaagaa ctgaaaaaac aaaatgcaaa atggtttttc caagccttac tatgcttgga
      301 agtaggtgtc aaaccaagca ctattaaacc ttctgaatat caagctttag aactaacgta
      361 tgggaaattt gtcgaaacga aaaaagccaa gactgtttca agtgaatggt tagattattt
      421 tgaaaatatc aataagtatg gcgcctatta caccatgaaa aaggaggaca acgaaaatga
      481 ataaggtgat ttatcaacta acaacggaag atgtgtcaca ctttttaaca aacaaagaac
      541 atttgaatga agtaatcaga cggattcaaa aagactttag ttgtatggaa gaaatgaagt
      601 actttattca aagagtcgaa gaacaattgt atttggaaag tgaaacgagt caaacgaagg
      661 tcacagtcag caataattat ttagacgtgt ataaacatcg tcctgaaacg gtaaaaaaag
      721 gaacggagct tctacgacta gaaacagaaa aatacatcat gaatgtttac ctagaaagga
      781 gtgaactggt cgaaaacttt gtcagctgca cgatttgcag gaaacactat tactcagaat
      841 cagaatatta ttcaattttt gagagcatgg aattaatgga agaagaaatg gaagagctaa
      901 acagcaaaaa tgcgacgata gacgattatt a
//
LOCUS       DAZKKW010000271          930 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.281, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000271 DAZKKW010000000
VERSION     DAZKKW010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..930
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.281"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..441)
                     /locus_tag="ACG8PO_003024"
     CDS             complement(<1..441)
                     /locus_tag="ACG8PO_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS6770 family transposase"
                     /protein_id="HFQ0208260.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQGKTALDYWHQYKENKKKCGRKVIQLPAHEVDYIKEKVTLGWTPDVIIGRKERPVS
                     CGMRTLYRLFSKGIFDIDTLPMKGKRKPNGHQEKRGKQQYQRSIH"
BASE COUNT          298 a          162 c          158 g          312 t
ORIGIN      
        1 atggattgag cgctgatact gttgttttcc ccgtttttcc tgatggccat tgggttttct
       61 tttacctttc atcggtagtg tgtcaatatc aaatattcct ttagaaaata aacgataaag
      121 tgttctcata ccgcatgaaa caggcctttc ttttcgcccg ataatgacgt caggcgtcca
      181 accaagagtg actttctctt taatgtaatc tacttcatga gcaggtaatt gaatgacttt
      241 tctaccacat tttttcttat tttctttata ctggtgccaa taatcaagag cagtcttgcc
      301 ttgcttgaac ttattgacta cattataaat agtttgtata gcacgtccca ttcggttagc
      361 gatttcaacc ggcttattat gttgaagata atatgattct atcattgtca gttcgtctat
      421 ggtaagatgt ttgtaggtca tttatggtta cactcctttg ttttctttcg tcggaaatac
      481 aatttgagtg taccataaat gcctttttat ttttctaact taattttaca attcgcgata
      541 tattaaacaa cgttgtcgcg cttcattgaa acattttatg aaaattccaa aaaagagttg
      601 acacatgttt ttagacgtga taagataact aagtcgttaa aaagaaccac ttgcgaagct
      661 tgattctatc gaaaaaaaac ttcaaaaaag ttcttgacaa ataacaaacg ataagttatg
      721 ataaatgagt tgctgaaaga tttcgaaaaa cgaaatcgaa aaaaactttt aaaaaagagt
      781 tgacaatcgc ttgtctctct gatatgatat aaaagttgct acggcagcca aaaacgaatc
      841 gggaaacact cccgatgaag tagatctttg aaaactgaac aaagtaagac aaaccaaatg
      901 tgtagggcgt cttgattcaa ttcaagacaa
//
LOCUS       DAZKKW010000272          928 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.282, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000272 DAZKKW010000000
VERSION     DAZKKW010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..928
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.282"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..155)
                     /locus_tag="ACG8PO_003025"
     CDS             complement(<1..155)
                     /locus_tag="ACG8PO_003025"
                     /inference="COORDINATES: protein motif:HMM:NF024059.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0208261.1"
                     /translation="MVDKQRNQHYPLVPISIILSALKEDPISIKMILKHYHGYITKLC
                     LKNGFNEV"
     gene            complement(149..550)
                     /locus_tag="ACG8PO_003026"
     CDS             complement(149..550)
                     /locus_tag="ACG8PO_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HFQ0208262.1"
                     /translation="MQQNNNEISTQHQFDAYCKKVLRNEAKSIRKRNDKIKENEEPLN
                     DSNEGKYSQNHLDEQDVYFLFGMEILISDQKLSAAIDQLSDTRKKIVLLYYFAGFNDT
                     EIGKIFNMSTSGIWYQRTKAVEQLKMEYGLW"
BASE COUNT          310 a          154 c          115 g          349 t
ORIGIN      
        1 acctcattaa atccattttt taaacataac ttagttatat aaccgtggta atgtttaagt
       61 atcattttga ttgaaattgg gtcttctttc aaagcagata agataataga tataggaact
      121 aatgggtaat gttggttcct ttgtttgtct accataagcc atattccatt ttcaattgtt
      181 caacagcttt cgttcgttga taccagatac cacttgtact catgttgaaa attttaccga
      241 tctcagtatc attaaaaccc gcaaaataat aaagtaaaac aatctttttc cttgtatccg
      301 ataactgatc aattgctgct gataacttct gatctgaaat taatatctcc attccaaata
      361 agaaataaac atcttgctca tctaaatgat tttgtgaata cttaccttca tttgaatcat
      421 ttaatggttc ctcattttct tttattttat cgtttcgttt tcgaatagac ttagcttcat
      481 tgcgtaatac ttttttacag taggcatcaa attggtgttg tgtagatatc tcattgttgt
      541 tttgttgcac gatcaatatg ctcctttatc ccctttccac taatacaaca atcagagaat
      601 acctattttt cgaaaaacta gaagaaaata aaaacgaact tcattttttg aatctacata
      661 tcctgttcca ctaacatcgt atatttcaca atagaataaa taggcttttg aaattatgct
      721 ttacttacaa gaaatcacag ttattttttg gaggaaaata aaattactat tcagaagata
      781 attaaaaata tcaatataac aaaaaaatca gcattcaatc ctaattgtct gattgaatgc
      841 tgattttcta tgctatacat gtagcttgag tattcctttt taattactca ccaataatca
      901 attaggaata ttactaaatt tggagtta
//
LOCUS       DAZKKW010000273          920 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.283, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000273 DAZKKW010000000
VERSION     DAZKKW010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 920)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..920
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.283"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..216
                     /locus_tag="ACG8PO_003027"
     CDS             <1..216
                     /locus_tag="ACG8PO_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011837604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HFQ0208263.1"
                     /translation="NQNTNSYQAAILTAVNLGGDTDTTAAVTGALAGILYGGEVIPEN
                     WRKSLQNDQLLKTISQRFCDTVLSQRI"
     gene            318..551
                     /gene="relB"
                     /locus_tag="ACG8PO_003028"
     CDS             318..551
                     /gene="relB"
                     /locus_tag="ACG8PO_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB family
                     antitoxin"
                     /protein_id="HFQ0208264.1"
                     /translation="MSQSTITFRIPDDEKELVLEYAKAHNSSLTELYRNAVLDKIEDE
                     IDLRTLRSAIEVSKEKKESGISQEEMEKLLNEL"
     gene            541..810
                     /locus_tag="ACG8PO_003029"
     CDS             541..810
                     /locus_tag="ACG8PO_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFQ0208265.1"
                     /translation="MNYKVIYMPQAQKQLKKMDRNQAKIIVAWIRKNLENTTDPRQHG
                     KGLTGNKSGEWRYRIGNYRILANICDSEIEIQIFSIGHRSTIYKR"
BASE COUNT          329 a          146 c          192 g          253 t
ORIGIN      
        1 aaccaaaata cgaatagtta tcaagcagcc atattgactg cggtcaattt aggaggggat
       61 actgatacta ctgctgccgt aactggtgct cttgcgggta ttctttacgg gggtgaagtg
      121 atccctgaaa attggagaaa atcgcttcaa aatgaccagc ttcttaaaac gatcagtcag
      181 agattttgcg ataccgtgct atctcaaaga atttgaataa tggatatgat gcaaatcttg
      241 tttgtactaa cgttgtattg acaaaaagga ttttatttag tacaatgtta gtacaaatga
      301 tgaacggagg tagaatcatg tctcaatcaa ccattacatt taggattccc gacgatgaaa
      361 aagaactggt attagaatat gcaaaagcac acaattcttc attgaccgaa ctttatcgaa
      421 atgctgtact ggataaaatc gaagatgaaa tcgatctacg gactttacgg agtgcaattg
      481 aagtttccaa agagaaaaaa gagagtggta tttctcaaga ggaaatggag aaactgctaa
      541 atgaattata aagtcatcta catgccgcaa gcccagaagc aattaaaaaa aatggacaga
      601 aaccaagcta aaataattgt cgcctggatc agaaagaatt tagaaaacac gactgacccg
      661 cgtcagcacg gaaaaggttt gactggaaat aagagtgggg aatggcgcta tcgaattgga
      721 aactatcgaa tattggctaa tatttgtgat tctgaaattg aaatccagat attctcaatt
      781 ggacatagaa gtacaatata taaaagataa ccgtttttgg aacgaagtag agtttcgaaa
      841 gtcaatgtat aatcttttga attttgcata ataccacagg tagaaactac ccgatactat
      901 cggttggttt ctgctttttt
//
LOCUS       DAZKKW010000274          914 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.284, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000274 DAZKKW010000000
VERSION     DAZKKW010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..914
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.284"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>914
                     /locus_tag="ACG8PO_003030"
     CDS             <1..>914
                     /locus_tag="ACG8PO_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0208266.1"
                     /translation="YMNLKKIFSVKIFIVYAFVSLLYLFPAFKNMEIFGFYDTAFHIN
                     RALSLESIFSSPINFETFRSYGMQVNNFYPWLTLYPLFLLIKFTNLAIGYNLFLYIVT
                     LITLFICHYVMYEITKKHVTSSFFAIIYTTSSFRSVEIFLRGAMGELLAMSILPLILL
                     GFIKLYDSKKESWVMLAISMTLLIYTHVLSVAMTMVMIIIALIIQFYRKKKIEIFLII
                     KLIKAAIVTLLLSLAFIGPMIEQTLYQDLNRPYVDILQKRAIYLGKEFLIGSIDSELL
                     SFGIGLTLLICLIILTFNFKKLGILSKIT"
BASE COUNT          294 a          121 c          127 g          372 t
ORIGIN      
        1 tatatgaatt tgaaaaaaat tttttctgta aaaatattta tagtttatgc atttgttagt
       61 ttattatatt tatttccagc atttaaaaac atggaaatat ttggctttta tgatacggca
      121 tttcacatta atcgtgcgtt aagtttagaa tctattttta gctcccctat taattttgaa
      181 acgtttagat catatggcat gcaagtaaat aatttttacc catggttaac tctatatcct
      241 ttatttttat tgatcaagtt tactaattta gcaataggct ataacctttt tctttatatt
      301 gttacgctca ttactctatt tatatgtcat tatgtaatgt atgaaattac caaaaaacat
      361 gttactagct cctttttcgc tattatatat acaacatctt cttttaggag tgtagaaata
      421 tttcttcgag gggcaatggg ggaattactc gcaatgtcaa tacttcctct tattttactc
      481 gggtttataa agctttacga ctcaaaaaaa gaatcttggg ttatgttggc gattagcatg
      541 actttattaa tttacactca tgttctttct gtagctatga ctatggtaat gattataatt
      601 gctttaatta ttcaatttta tcggaaaaag aaaatagaaa tcttcttaat tattaaatta
      661 ataaaagctg ctatagttac tttacttctt tcactagcgt ttataggtcc tatgattgag
      721 cagacattgt atcaagatct gaatagacct tatgtggata tattacaaaa aagagcaatt
      781 tatctgggta aagagttttt gattggaagt atagattcag aattactatc atttggtatt
      841 ggcttaactt tgcttatttg tttaattata ctaacattta actttaaaaa attaggaatc
      901 ttatctaaaa tcac
//
LOCUS       DAZKKW010000275          906 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.286, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000275 DAZKKW010000000
VERSION     DAZKKW010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..906
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.286"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>906
                     /locus_tag="ACG8PO_003031"
     CDS             <1..>906
                     /locus_tag="ACG8PO_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFQ0208267.1"
                     /translation="LNIAISVALMQTLYFDDKYVGRVVGGTLSFVLIGLVIYLRIMIK
                     GAHFFDKKQWRYALKISLPLIPHSLANIVLAQFDRIMINSYLGPAAGGIYSYISNIGI
                     ILSVIWMSTNNAWVPWFYGEMSKNNEKKVKKVSNYYLVVFTLITMIVMVVSIDLAKFM
                     APKDYYSGLSLVVPITLGYFFQFLYSLPVNAEFYEKKTSFIAIGTVASAIVNILLNMI
                     FIPRLGIIAAGYTTVFTYFLLFIFHYNIAKKITNKQLFDTKKICIIIIFVSIFSLILH
                     ILINQMLIRYVLTSLLIISLVIYFLK"
BASE COUNT          293 a          110 c          150 g          353 t
ORIGIN      
        1 ttgaatattg caatctctgt agctttgatg cagacattat attttgatga taaatatgtt
       61 ggcagagtag tcggcggaac actgtcattt gtgttgatag gtttagtaat ttatttaaga
      121 ataatgataa agggtgctca tttttttgat aaaaaacagt ggagatacgc tttaaaaatc
      181 tctttgccgc taatcccaca ttccttagca aatatagttt tagcacaatt cgatagaatt
      241 atgattaatt cttatttagg tcctgctgcg gggggaatat attcctacat tagtaacata
      301 ggtattattc taagcgtaat atggatgtct actaataacg cctgggttcc atggttctat
      361 ggtgagatga gtaaaaataa tgaaaaaaaa gtgaagaaag ttagtaacta ttatttagta
      421 gtctttactt tgattacgat gattgtaatg gtagtttcga ttgatttagc aaaatttatg
      481 gctccgaaag actattattc tgggttatct ttagtggttc cgataacctt aggctatttc
      541 tttcagtttt tatatagttt accagttaat gctgaatttt atgagaaaaa aacatcgttt
      601 attgcgattg gtacagtagc aagtgctata gttaatatac tattaaacat gattttcata
      661 cctaggttag gaataattgc tgctggatac acaaccgtat ttacgtattt tttacttttt
      721 atttttcatt ataatatcgc taaaaaaatt acgaataaac aattatttga cacaaaaaaa
      781 atatgtatca taataatttt tgtatcaatt ttttctttga tacttcatat tttaatcaat
      841 caaatgttga ttcgttacgt tttgacatca ttattgataa ttagtttagt tatatatttt
      901 ttaaag
//
LOCUS       DAZKKW010000276          905 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.287, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000276 DAZKKW010000000
VERSION     DAZKKW010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..905
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.287"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..481
                     /locus_tag="ACG8PO_003032"
     CDS             <1..481
                     /locus_tag="ACG8PO_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0208268.1"
                     /translation="EVFVGDKCIFFDQPLLSDGYGIDETDIFAKVNSIFSGKKETLIK
                     LHPRARTNRYGEKNILNSSLPFEVLLLENDFSSTLLISPISTISFSPSMMFEKTINSI
                     MLAKLILNEYTALSEDKKETLTNIVLFCEHYNRLSEEKILLPENWAELRKLVEYGII"
     gene            468..>905
                     /locus_tag="ACG8PO_003033"
     CDS             468..>905
                     /locus_tag="ACG8PO_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFQ0208269.1"
                     /translation="MGLFNTLSKSKLVKSSFWYTIGNFFIKGISFITIPIFVNITTQA
                     EYGLINNFTSFVSIFSILVGLSLNSAINNASFDYKDDINEFMSSVLSLATLSLIVFLL
                     IGNIYFLFSNAFFDMNQLVFNLMLLQSFASFVISYIQSYFTINF"
BASE COUNT          298 a          121 c          132 g          354 t
ORIGIN      
        1 agaagttttt gtcggagata aatgtatctt ttttgatcaa cctttattat ctgatggtta
       61 tggcatagat gaaacagata ttttcgctaa agtaaatagt atattttctg gaaaaaaaga
      121 aactttaatt aagcttcatc ctagagcaag gacaaatcgt tatggtgaaa aaaacatatt
      181 gaattccagt ttaccgtttg aagtcttgct acttgaaaat gacttctctt caacactttt
      241 gattagtcct atatctacaa tttctttttc accatctatg atgtttgaaa agacgatcaa
      301 ctctattatg ttggctaaat taattttaaa tgaatataca gcattatctg aagataaaaa
      361 agaaacattg acaaatattg ttcttttctg tgaacattat aaccgtctat cagaagaaaa
      421 aattttactc ccagaaaact gggcagaatt aagaaagttg gtagagtatg ggattattta
      481 acacactttc caagtcaaaa ttagttaagt ccagtttctg gtatactata gggaattttt
      541 ttattaaggg aatttcattc ataactattc ctatttttgt aaatattact actcaagcag
      601 aatatggttt gattaataat ttcacttcat ttgtatcaat tttttcgatt cttgttgggc
      661 taagcctaaa ttcggcaatt aataatgcaa gttttgacta taaagatgac ataaatgaat
      721 ttatgtcatc ggtactttct ctagctacac tttcgttaat tgtgttctta ttaataggga
      781 acatatattt tttgttttca aatgcttttt ttgatatgaa ccaacttgta tttaatttga
      841 tgttattaca aagttttgct agttttgtaa ttagttatat ccagtcttat tttacgatta
      901 acttt
//
LOCUS       DAZKKW010000277          903 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.288, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000277 DAZKKW010000000
VERSION     DAZKKW010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..903
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.288"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          349 a          103 c          113 g          338 t
ORIGIN      
        1 attagcaata tataaaagaa ggtatcaaga gtcacagtga atttattccg aaaagtgaaa
       61 agagcaaata taaaatttaa taaaaataag aactaaatag gaaaacgtag acgtaatttt
      121 ctacgttttc cttttcatat atattaaata ttacggaaaa ttatataaac taatttagtg
      181 ttttgtttgt aattcctaat tttgctccac ttttagataa tttttaggtc tatttaactc
      241 attagacatt ctatgctcat ttttctccaa atttgtttca actctttcgt acttaatttt
      301 gcgtatttga aaattcttct tttagagtac ttttattacg atactagtat gatatatatc
      361 tacagcaaac agttaaaatc ttcctaaaat aagcgaatag cattcctaaa acctataaaa
      421 tttttagatg aagcgacaag tggacaatta ataaactact tgaacatgaa attatggatc
      481 acctttcaaa tttagaaaat ggaattctta tacttattgc gcaacattta ttgattgcaa
      541 aattatgtga caaaaagaac tagtagaaga aggaataatg gcttgataat aaagtactac
      601 ctattaaagg tagtacttta tttaatttag tataaattaa catactacta tattttaggt
      661 ttcatattat ataaattaat aatatactac catttgttgg tagtataatt taaaggtaat
      721 aagttattct tttattacta tgtttgggtt ttatagcaaa gaaagtttaa taagagtttg
      781 gacctagcaa acaatattaa tgaggtcatt aaatcttatt atctttagtg atataatgaa
      841 atatctacac gacaaaatta tgatcttgcg taaagttata aaatattaat aaaaatattt
      901 tat
//
LOCUS       DAZKKW010000278          888 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.289, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000278 DAZKKW010000000
VERSION     DAZKKW010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..888
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.289"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>888)
                     /locus_tag="ACG8PO_003034"
     CDS             complement(<1..>888)
                     /locus_tag="ACG8PO_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFQ0208270.1"
                     /translation="FAKEQFTSVSTVFRYAKLLIPYFRRYHITFHPFQLELNASEANI
                     RSFFYYFYWNSTRESSDKWPFHIEQKEIEKYIVAFEGIYDITLTIFQKRVFSFWLAIN
                     IERSSFRKVRVDNEYKSVISDDPHFNLLKKWSKQINLSFNSDELCFLYRIIYSFGVID
                     GNAIYENSHAYAHQRQNTCSYRAVENLEKVLQSMFRFSLDIKDPELIFNFIAFHERSY
                     LFYGNPDLFFNRSYIEEMKEEEPRTYHIMEKLKKELQANADLDVSKKLENWAQLFLDY
                     YYVLDYYDLFLTNVKPIKI"
BASE COUNT          323 a          146 c          111 g          308 t
ORIGIN      
        1 aaaatcttaa tcggtttaac atttgttaaa aataaatcat aataatctaa cacataataa
       61 taatccaaaa ataattgtgc ccagttttct aatttctttg atacatccaa atccgcattt
      121 gcttgtaatt cttttttgag tttttccatt atgtgataag ttcgaggttc ttcttctttc
      181 atttcttcga tatacgaacg attaaaaaaa agatctggat taccataaaa tagataactt
      241 ctttcatgaa atgcaataaa attgaatatg agttctggat cctttatatc taaagaaaaa
      301 cggaacatgc tttgtaaaac tttctccaaa ttctcaactg ctcgataaga acaagtattt
      361 tgtcgctgat gtgcatatgc atgactattt tcatatattg catttccatc aattactcca
      421 aaagaataaa ttattcgata aaggaaacac agttcatcgc tgttaaaaga aagattgatt
      481 tgttttgacc acttcttcaa aagattaaaa tgcggatcat ctgagataac tgatttatat
      541 tcattatcta ctcgtacttt tctaaatgaa cttctttcta tattaatggc taaccaaaaa
      601 gaaaatacac gtttttgaaa aatagttaac gtaatatcat aaatcccctc aaaagcaact
      661 atatacttct ctatttcctt ttgttcgata tgaaatggcc acttatcact actttcacgt
      721 gtagaattcc aatagaaata ataaaaaaag ctacgaatgt ttgcctctga tgcattcaat
      781 tctaactgaa atggatgaaa tgttatatga taacgcctaa aatatggaat taagagcttc
      841 gcatatctga aaacagtaga tacactagta aattgctctt tagcaaaa
//
LOCUS       DAZKKW010000279          877 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.290, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000279 DAZKKW010000000
VERSION     DAZKKW010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..877
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.290"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>877
                     /gene="bsh"
                     /locus_tag="ACG8PO_003035"
     CDS             <1..>877
                     /gene="bsh"
                     /locus_tag="ACG8PO_003035"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HFQ0208271.1"
                     /translation="GRNFDYEISYNEVVTVTPRNYKLNFRKVNDLDTHYAMIGIAAGI
                     ADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFIPWILGQCSTVGETK
                     KLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHIYDNPVGVLTNNPSF
                     DYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDLSSVSRFVKATFTKL
                     NSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSCCNVDKGIYYYRTYE
                     DSQITAIDMNKEDLDS"
BASE COUNT          303 a          116 c          162 g          296 t
ORIGIN      
        1 tggaaggaat tttgattatg aaatatctta caatgaagta gttactgtta ctccaagaaa
       61 ttataagttg aattttcgaa aggtaaatga tttggatact cattatgcaa tgattggtat
      121 tgccgctggt atagctgact accctcttta ttacgatgcg acaaatgaaa aaggattgag
      181 tatggctggg ctaaattttt ctgggtatgc tgattataaa gaaatacaag aagggaaaga
      241 caatgtatct ccttttgaat ttattccttg gattttagga caatgctcaa cagtaggaga
      301 aactaaaaaa ttgttaaaaa atatcaattt agcaaatata aattatagtg atgaacttcc
      361 tttatcccct ttacattggc tattagctga taaagaaaaa tcaattgtca ttgaaagtat
      421 gaaagatgga cttcatatat atgataaccc tgtgggcgtt cttaccaata atccttcatt
      481 tgactatcaa ttatttaatt taaacaatta tcgtgtctta tcgagtgaaa ctcctaaaaa
      541 taatttttca aatcaaataa gtttgaatgc ctatagccgc ggtatgggag ggataggctt
      601 gcctggagat ttatcctcag tatctcgttt tgttaaagcg acttttacga agctgaattc
      661 tgtatctgga gattcagagt cagaaagtat tagtcaattt ttccatatct taggttcagt
      721 agaacaacaa aaaggtttgt gtgatgttgg tgatggaaaa tatgaatata caatttattc
      781 ttcttgttgc aatgttgaca aaggaatcta ttattatcga acatatgaag acagtcaaat
      841 tactgcaatt gatatgaata aagaagactt agatagt
//
LOCUS       DAZKKW010000280          872 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.291, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000280 DAZKKW010000000
VERSION     DAZKKW010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..872
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.291"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            38..652
                     /locus_tag="ACG8PO_003036"
     CDS             38..652
                     /locus_tag="ACG8PO_003036"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HFQ0208272.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            668..>872
                     /locus_tag="ACG8PO_003037"
     CDS             668..>872
                     /locus_tag="ACG8PO_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0208273.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
BASE COUNT          329 a          145 c          170 g          228 t
ORIGIN      
        1 tgctttttaa cgaattttat agaaagaagg cgaaaccatg tttgaacgga gtccttcaga
       61 attgacagaa cgtgaaaatt acaagttgct gattggttca gtcatcccta gaccagtagc
      121 agtagtaacg actaaatcag caaaagatat agtgaatatc gcacccttca gttacttcaa
      181 tatcgtcagt tcgaatcgac ccatcttatc cttggcgatc cagagaaaac aagatgcagt
      241 aaaagacaca gcaaagaata ttcttgagac aagagaagct gttatccata tccttgatga
      301 ggacaacgta gaagaagtaa ataaaacagc agccaatctg cctaatgagg aaagcgaact
      361 gtacctgact gacttgactt taactgattc agtaatcatt tcagtaccag cattgacaga
      421 agcaaaagtt cgttttgaaa catccttata tgaacatgtc gagatacgga ataaaaataa
      481 agtaacagct gatttatttt tgctagaagt acagaaatat catttaaatg aaacagtcta
      541 tgatgataag acaggaagga tcgatccaag aaaattgaat gctgtaagcc gtttggcagg
      601 aaatgattat gcagcaatag gagaaatttt cacgattgca cgtcccgaat aaaaaggagc
      661 actagttatg ttaaaaataa gctataaacc atcgacagat tcaaaagaaa tgaaaaaaga
      721 atatgaaaca gtgaatgatt ttctgcaagg gcagtactta gaagttccgc cattgcaaga
      781 ccattttgtc gttactactg ttactttaga tggtaaggaa atagaaatgc cagatcaaac
      841 gattagcgga ttgtttaatt actttaacaa at
//
LOCUS       DAZKKW010000281          867 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.292, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000281 DAZKKW010000000
VERSION     DAZKKW010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..867
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.292"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..182
                     /locus_tag="ACG8PO_003038"
     CDS             <1..182
                     /locus_tag="ACG8PO_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0208274.1"
                     /translation="ILTIPEGYRPPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRN
                     SSYGSDYTFAITYTI"
     gene            199..348
                     /locus_tag="ACG8PO_003039"
     CDS             199..348
                     /locus_tag="ACG8PO_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0208275.1"
                     /translation="MKAAYRPIEPYGFEQIIVNDEEHLPEECTEVEPPIPNWKPKFNY
                     WEGNK"
     gene            345..791
                     /locus_tag="ACG8PO_003040"
     CDS             345..791
                     /locus_tag="ACG8PO_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0208276.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGIRADGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYESLENENGDLKQINAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            793..>867
                     /locus_tag="ACG8PO_003041"
     CDS             793..>867
                     /locus_tag="ACG8PO_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HFQ0208277.1"
                     /translation="MFSFSDVKMMYDWGCFTNEQVMVFV"
BASE COUNT          308 a          148 c          187 g          224 t
ORIGIN      
        1 cgatactaac aattccagaa ggctatcggc caccaattag ttttgagcaa ctggttggct
       61 cgatagaccg ttctactttg aacagtgctc agttatctat tggtgcagat ggagccatta
      121 aatggcgaag aaactcaagt tatggatcgg attatacctt tgcaattact tacacgattt
      181 agaaaagcgt gaatcgatat gaaggcagca tatagaccaa ttgaacctta cggattcgag
      241 caaatcattg tgaatgatga agaacattta ccggaagaat gcacagaagt cgaaccaccg
      301 attccaaatt ggaaaccgaa attcaattac tgggagggaa ataaatgaaa aacatttgga
      361 aatatggacg tactggcgga gagtacgcag gaaaagtatt ggacgacatg cttgtatccg
      421 ttccttacac agatcagcca ccgcttgaag gaattcgtgc tgatggcgaa ccgttaacaa
      481 tcgctgatca aatgtttgat cctaaattga atcaatggat tattttagcg aacgcactag
      541 atcacaacga tttaaacaat ctcaaagcaa tgtatgagtc gttagaaaat gagaacggcg
      601 atttaaaaca gatcaatgcc aaactcatgc taagcgatgt agcaattaaa caggaaaata
      661 ctgcattgaa agaaaaagcg gatagtttag cacaaatcaa ttcaaaaatg atgcttgctt
      721 cgttacaaaa tagcaaagac atttcagaaa ttaaagagca actaaatcca gcttcaaagg
      781 gaggtgagta gtatgtttag ttttagcgat gtgaaaatga tgtatgattg gggctgtttc
      841 actaacgaac aagtaatggt tttcgtt
//
LOCUS       DAZKKW010000282          864 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.293, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000282 DAZKKW010000000
VERSION     DAZKKW010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..864
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.293"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(201..600)
                     /locus_tag="ACG8PO_003042"
                     /pseudo
     CDS             complement(201..600)
                     /locus_tag="ACG8PO_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
     gene            complement(597..833)
                     /locus_tag="ACG8PO_003043"
     CDS             complement(597..833)
                     /locus_tag="ACG8PO_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFQ0208278.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
BASE COUNT          269 a          157 c          121 g          317 t
ORIGIN      
        1 taaaaaatca gcccactagt tctaaaccac tatttcctac tattcactct tttgtattcc
       61 ttttaaatca attatttcaa aattaatcca tattttccat atttttatga aaaaactatt
      121 ttgtatgatt aaacataaaa aaatcagcat attccttaat gacatgctga tctaattgag
      181 aaataaagtt tttaattcgt tcattttatt gcggtttgtc gtaaataact ggttatcttg
      241 gactttagtg aatcgaaatc caatgaacta gtggtaatca ttatgataaa atcaacagct
      301 tcttgacgag aaaaagcaac tggacaatta ttcaattgaa gaaacaacaa catggcaaca
      361 agagcagttc gtttattgcc attatgaaaa ggatgttttt tagctaaatt aattgctaaa
      421 atcgccgctt tttcatagat ttcaggatag agttcttgat tgaatactgc ttgtgaaggc
      481 tgtttgacgg ccatatctaa tgcatttgca tcttttacac cgatcatttc gcctggcgag
      541 tgacggtaaa ataactttcg cgttgatttt gataatttct ttagtcgaaa gatacctcat
      601 aaatcaacca aatcttttaa cgcgtcttca tattgattca tgatcgaatc aactaatgaa
      661 tcaatatcag gttcttcttg tgctttgatc attcgtacag aaccatcaga ttgaaaaaca
      721 taactgattg cgtcacctgt ctgcacaccc atgaattctt ttacttctgg tgggatagtt
      781 gttacatcgc tattgccaga ttttctaatt ttatactttt tcattttttt catatttcgc
      841 caccctctat accattctca ttct
//
LOCUS       DAZKKW010000283          832 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.294, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000283 DAZKKW010000000
VERSION     DAZKKW010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..832
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.294"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..693
                     /locus_tag="ACG8PO_003044"
     CDS             <1..693
                     /locus_tag="ACG8PO_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HFQ0208279.1"
                     /translation="ATISRHSSGKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILS
                     DGQKIDNNKFTSKMEKKLKREQRKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEK
                     VMNQRTDFLNKLSTEMIKNHDIICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYK
                     ADWYGRKIIKVDKWFPSSQICSECGHKDGKKSLEIREWTCPVCHTHHDRDINASINIL
                     TEGLRLHSMGLA"
BASE COUNT          304 a          132 c          154 g          242 t
ORIGIN      
        1 gctacaatat cacgtcattc tagcggtaaa tactatattt ctttgttgtg taaagaagaa
       61 attatcgaat tacctaaaac taattccaca attggtattg acttaggtat tatggacttt
      121 gcgattcttt ctgatggaca aaaaattgat aataataaat tcacgtccaa aatggaaaag
      181 aaactaaagc gtgaacaacg caaattgtct aggcgtgctt tattggctaa aaataaaggt
      241 accaatctat ttgaagctaa aaattatcaa aaacaaaaac gcaaagtagc tagattgcat
      301 gaaaaagtaa tgaatcaacg tactgatttt ctgaataagt tgagtacaga aatgatcaaa
      361 aatcacgata ttatctgtat tgaagactta aacacaaaag gtatgttgcg aaatcataaa
      421 ttagctaaaa gtatttctga tgtttcttgg tcaaagtttg tgactaaatt acaatacaag
      481 gctgactggt atggacgaaa aatcatcaaa gtagataaat ggtttccgtc tagtcagatt
      541 tgttcagagt gcggacataa agatggcaag aaatctcttg aaattcgaga atggacttgt
      601 cctgtttgtc atactcatca tgatcgtgat atcaatgcta gtatcaatat tctgaccgaa
      661 ggtctaagac tacattctat gggattggct tagatccaat catcaagaac cgtaggaact
      721 acggggatag cttggtaaat aagagacacc gctgttagta aagaaatacg ctatcaagta
      781 tgctctattc ccaagaagct cccacttcaa gcgttaagaa ccgtcaggtt ta
//
LOCUS       DAZKKW010000284          797 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.295, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000284 DAZKKW010000000
VERSION     DAZKKW010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..797
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.295"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            601..>797
                     /locus_tag="ACG8PO_003045"
     CDS             601..>797
                     /locus_tag="ACG8PO_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFQ0208280.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTL"
BASE COUNT          294 a          126 c          105 g          272 t
ORIGIN      
        1 attataaaat atccacttat cgctttcatg tatattccac cgtaaaaagt acattttatc
       61 accgagaact catcattttt catcatgatg aatactattt acatatatct agttcaaaga
      121 caataattat tatgatccgt tattatctaa gcactataca gataatagga atttttcgtt
      181 tctaaatatt ccaatttggg aatccaataa aaattttcgt tcaaatacgt gtctattcga
      241 actcttttct agttgttaat tatgaagggg gaagaaataa aaaatacaat ctatacacga
      301 ataagaatat tatccaaaag gaggttttag aaaagattta ttatacgtaa aaagctcaag
      361 acattttctt agccataagg acatgtttga tatttaagaa gatatatgaa ttttcttttc
      421 taagtataaa gataactatc cattatcaac taaaaaaaca tttttgaaaa taaacatcag
      481 cagtcctatc aatgcatgtg agcttataga agttattcgc agcgcagcat aattgcatta
      541 gcttcttttc gtgttttttg cctcttttcc ctttaaatct taatggagga ggtgtgcaat
      601 atgtcagata acgacgaacg cgtaaatgcc cagttgattc gattctttga aaaggtcatt
      661 ttgaacaccg ctaatactta ttataaaaaa caagaagaaa ttcaaaaaca tgaacaactt
      721 gatcaactcc caccatattt gtttactaaa gatactataa aatttaaaca accgattaat
      781 gttttaaatg tgactct
//
LOCUS       DAZKKW010000285          788 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.296, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000285 DAZKKW010000000
VERSION     DAZKKW010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..788
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.296"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..346)
                     /locus_tag="ACG8PO_003046"
     CDS             complement(<1..346)
                     /locus_tag="ACG8PO_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0208281.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYTTN"
     gene            complement(363..767)
                     /gene="tnpA"
                     /locus_tag="ACG8PO_003047"
     CDS             complement(363..767)
                     /gene="tnpA"
                     /locus_tag="ACG8PO_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HFQ0208282.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLWKE
                     MFWSKSFCLLTTGGAPIDVIKKYIQNQGNNHK"
BASE COUNT          234 a          129 c          126 g          299 t
ORIGIN      
        1 tattcgttgt ataactctga acaggatttt tcttactttt gaaacgtgga aatccaacgg
       61 atttatcccg aaaaaaattc ttgtatgctt tatctaaatt aagttgggca ttggctaaag
      121 caaggctatc aatttctttc aaaaacggaa attctttctt gtatttagct ggtgtcggaa
      181 aagtcatttt tttagaagaa tcatttttaa cttcttcata cgcctttttt cggtcatcaa
      241 gcattagatt gtagacctta cggacacaac caaaagattt ggcaaagaag atttcttgtt
      301 cctctgttgg atagattctg aatttgtatg cttttagttg ttccataaag ttcacctact
      361 ttctatttat gattgtttcc ttgattttgg atatacttct taatcacatc aattggtgcg
      421 ccaccagttg tcagaagaca aaaactttta gaccagaaca tttctttcca aagaaattgt
      481 ttgactcttg gaaagtcacg ttttatcaat cttgagctgg cacttttata ggcattgatg
      541 aattttgtca attctgtttt aggttgagct ttgaacataa tatgaacgtg gtctttatca
      601 tgattccact ccactaaagt aatgtgatac gattctgaaa ttctttcaaa agtagttttg
      661 gcataatcag atatttcatc atcaatcact tgtctacgat atttcgttac taaaacaagg
      721 tggtaataaa gaagaaatac tgaatgatta tttgtatcta attccatgtt atatcaactc
      781 atttcttg
//
LOCUS       DAZKKW010000286          775 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.297, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000286 DAZKKW010000000
VERSION     DAZKKW010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..775
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.297"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>775
                     /locus_tag="ACG8PO_003048"
     CDS             <1..>775
                     /locus_tag="ACG8PO_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ArdC-like ssDNA-binding domain-containing
                     protein"
                     /protein_id="HFQ0208283.1"
                     /translation="PTDSLKEKEKDHFYRVEFNENDPEKGLKSYGGEIVTLELIDQLK
                     TLDNQYAAEAGYYKFYFEEVKNGEVVDQIRMDLGDGVNSNHPIYLELAKLCEQVPEKE
                     QKQSVKNEPVSKEQMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSSE
                     DVKELLNFMSRFPERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVR
                     KTVKGFYEKGRGFVRETEATPEDKKRIESGQIEVIKKPY"
BASE COUNT          310 a          126 c          168 g          171 t
ORIGIN      
        1 caccaactga ttctctgaag gagaaagaaa aagatcattt ctatcgagta gaattcaacg
       61 aaaatgatcc agaaaaagga ttgaaaagtt atggtgggga gatcgtaaca ttagaattga
      121 tcgatcaact taaaacgttg gataaccaat acgctgcaga agcaggttat tataaattct
      181 attttgaaga agtgaagaac ggagaagtag tagatcagat ccgaatggat ctaggggatg
      241 gcgtcaacag taatcacccc atctatttag agttagcaaa attgtgcgaa caagtacctg
      301 agaaggaaca aaaacaatcg gtcaaaaatg aacctgtttc gaaagaacaa atgcagtcga
      361 aagaaacaaa aacaaaacaa aagggctatc aaaaacgaac ggcagaagag atcaaacaag
      421 aagttaaatc cctttcgcaa caggcactgg atgcggtgaa aaagcatacc cattcttctg
      481 aagacgtcaa agaacttttg aactttatgt ctcgttttcc agaacgatcc tttagaaatc
      541 aattattgat tgaggaacaa tttccaggtg caacggcttg tttaggtcgt gccgcgttga
      601 aaaaagaagg aatctatatc aaaaaagggg aacaagggaa caagatcttt gtcagaaaaa
      661 cggtcaaagg attttatgag aaaggacgtg gctttgttcg agaaacagaa gctacaccag
      721 aagataaaaa acgaatcgaa agtggtcaaa tcgaagtgat caaaaaacct tacta
//
LOCUS       DAZKKW010000287          775 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.298, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000287 DAZKKW010000000
VERSION     DAZKKW010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..775
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.298"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>775
                     /locus_tag="ACG8PO_003049"
     CDS             <1..>775
                     /locus_tag="ACG8PO_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ArdC-like ssDNA-binding domain-containing
                     protein"
                     /protein_id="HFQ0208284.1"
                     /translation="PTDSMKEKENDHFYRVEFNENDPEKGLKSYGGEIVTFELIDQLK
                     TLDNQYAAEAGYYKFYFEEVKNGEVVDQIRMDLGDGVNSNQPIYLELAKLCEQVPEKE
                     QKQSVKNEPVSKEQMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPE
                     DVKELLNFMSRFPERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVR
                     KTVKGFYEKGRGFVRETEATPEDKKRIESGQIEVIKKPY"
BASE COUNT          309 a          127 c          170 g          169 t
ORIGIN      
        1 gaccaactga ttctatgaag gagaaagaaa acgatcattt ctatcgagta gaattcaacg
       61 aaaatgatcc agaaaaagga ttgaaaagtt atggtgggga gatcgtaaca ttcgaattga
      121 tcgatcaact caaaacattg gataaccaat acgccgcaga agcaggttat tataaattct
      181 attttgagga agtgaagaac ggagaagtag tcgatcagat ccgaatggat ctaggggatg
      241 gcgtcaacag taatcaacca atctatttag agttagcaaa attgtgcgaa caagtacctg
      301 agaaggaaca aaaacaatcg gtcaaaaatg aacctgtttc gaaagaacaa atgcagtcga
      361 aagaaacaaa aacaaaacaa aagggctatc aaaaacgaac ggcagaagag attaaacaag
      421 aagttaaatc cctttcgcaa caggcactgg atgcggtgaa aaagcatacc cattctcctg
      481 aagacgtcaa agaacttttg aactttatgt ctcgttttcc agaacgatcc tttagaaatc
      541 aattattgat tgaggaacaa tttccaggtg caacggcttg tttaggtcgt gccgcgttga
      601 aaaaagaagg aatctatatc aaaaaagggg aacaagggaa caagatcttt gtcagaaaaa
      661 cggtcaaagg gttttatgag aaaggacgtg gctttgttcg agaaacagaa gctacaccag
      721 aagataaaaa acgaatcgaa agtggtcaaa tcgaagtgat caaaaaacct tacta
//
LOCUS       DAZKKW010000288          750 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.299, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000288 DAZKKW010000000
VERSION     DAZKKW010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..750
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.299"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..456
                     /locus_tag="ACG8PO_003050"
     CDS             <1..456
                     /locus_tag="ACG8PO_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0208285.1"
                     /translation="AERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQEH
                     KNTLETPENKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGKS
                     YGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            517..>750
                     /locus_tag="ACG8PO_003051"
     CDS             517..>750
                     /locus_tag="ACG8PO_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0208286.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQD"
BASE COUNT          290 a          110 c          160 g          190 t
ORIGIN      
        1 gcggaacgtg ggaaaatcat gaatgatgtc agccgtgtcg caaatgaatt cattcaaacc
       61 atttctaatg aaatcaatca atacagagaa agagaaccag agatccaacc agcagaagtg
      121 aagcaggagc ataaaaatac tttagaaacg ccagagaata agaatattgt gcccgttggt
      181 tcactttgga tcgacaagaa agacgggaaa gtaatggaag aagatacggg tcgaacgtta
      241 catttgaagg atgcggcgtt tattgaagat ccgcagcata atcaagtaat tttaaaagga
      301 aaaagttatg gaaaagagat ccaaacggcg ttaccttcag aagaatttgc gctaggaaat
      361 ccattccgat tttcacttga gacagtgtca gaagtggcaa aacgaaaaaa tgaaatctat
      421 tatgaacaac aattgtcaca aaaagaacgt gtataaaaca agtaaaaaag actatctgaa
      481 aaacggatag tcttttttct tttgggaggt tttaaaatga ccgagaaaaa aatgagtctt
      541 attgatcgat gtaaacagat cgatatagtg gattttgctc gaaacaatgg aatggctgta
      601 gtcaataaag gacgagatta tcgcttagaa gaccatgatt catttgtttt tgatcgaaga
      661 aaacaacgtt tttattggaa cagtcaaaat attagtggcg acattattga attagctaaa
      721 ctttttttca tagacaaaga gatccaagac
//
LOCUS       DAZKKW010000289          696 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.300, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000289 DAZKKW010000000
VERSION     DAZKKW010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..696
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.300"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(54..629)
                     /locus_tag="ACG8PO_003052"
                     /pseudo
     CDS             complement(54..629)
                     /locus_tag="ACG8PO_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
BASE COUNT          231 a          150 c          124 g          191 t
ORIGIN      
        1 aatgaaaaaa cagagccaga ttagcattat tttgctaatc cagctctgtt tattcagcct
       61 atttttcttt ttgaatatat caagcgttgg ggcaacatag tgatgactaa tgaaacaacg
      121 acgatagaca acaagcgatc aaggtagtct gtacccgcct gtaccaagat cacacttgtt
      181 gtctgactta aacctaaccc atgtaatagc tgaacgatca tactggatcc tgaggaggtg
      241 atcccgccaa ataactgaac ggtaatgatc gaagaaacga tcgttccagg taccgacaaa
      301 atcaaagcga gtaataacag tttcaagctg tttttatctt tgccgagata tcttggcaaa
      361 atcaatccag caagcaagcc tgtgagaatt tggattggcg aataatacag agaaaaaata
      421 tctgtagtca ctccacttaa aataccgctg gacaatcctg tcagcattca aggtacaggc
      481 ccaagcaatg caccagcaaa aatcgttccg atagaatcga gatagattgg caaacgaagc
      541 cataaagcaa tcgttcctcc aatataattg atcccaatac acaaagccat cactgtaagt
      601 acgcgagttg ccattttgtt tttattcatt ttgttagccc atctttaacc tttccaaatc
      661 tttcataatc gtttcttctt gtgcatttaa aagaac
//
LOCUS       DAZKKW010000290          691 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.301, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000290 DAZKKW010000000
VERSION     DAZKKW010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..691
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.301"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>691
                     /locus_tag="ACG8PO_003053"
     CDS             <1..>691
                     /locus_tag="ACG8PO_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS982-like element ISEfm1 family transposase"
                     /protein_id="HFQ0208287.1"
                     /translation="ITFKEIMRTVSNIYHNCVPDKIKNRRNTDQLKQRDTVIIACVIW
                     GIINGYTSQRATYRAVCSVLFPNGDFPSRSRFTRLSSNLAYTIKIIRYFFIKKLTKGE
                     LVGIIDSFPSPLCKPVRNRQAKLLNQIAKVGYNSTKKSYFYGLKIHMIVTKTGFPITY
                     SITNPGVHDVKVLETLSEEANLPNILGDKGYISHKIHEKLALKGITISVPPRKNMDKS
                     EKLDHSLLGKQR"
BASE COUNT          247 a          129 c          119 g          196 t
ORIGIN      
        1 ataacattca aggaaattat gagaactgtt tctaacatat accacaattg tgtaccagat
       61 aagattaaaa accgaagaaa tactgatcaa ctgaagcaac gcgatacagt gattattgct
      121 tgtgttatat ggggcataat taatggctat actagccaaa gagccacgta tagagcggtt
      181 tgttccgtct tatttcctaa tggtgacttt cctagtagga gtcgattcac tcgcttaagt
      241 tcaaacttag cttacactat caagattatt cgatactttt tcatcaagaa gctcaccaaa
      301 ggtgaactag tcggaattat agatagtttt cctagtccat tatgtaaacc agttagaaat
      361 agacaagcaa aactattgaa tcaaatagcc aaagttggct ataactcaac aaaaaaatct
      421 tatttttatg gtcttaaaat tcatatgatc gttactaaga ctggctttcc gattacttac
      481 tcaatcacga atccaggtgt ccatgacgtg aaagtgttag agactttatc ggaagaagcg
      541 aatcttccca atatcctagg ggacaaagga tatattagcc ataaaatcca tgaaaagctt
      601 gcccttaagg gcattactat atccgttccg ccacgtaaaa acatggataa atcagaaaaa
      661 ctagaccaca gcttacttgg aaaacaaaga a
//
LOCUS       DAZKKW010000291          637 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.302, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000291 DAZKKW010000000
VERSION     DAZKKW010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..637
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.302"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            51..>637
                     /locus_tag="ACG8PO_003054"
     CDS             51..>637
                     /locus_tag="ACG8PO_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="HFQ0208288.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSH
                     TTIYRWVQEYGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDI
                     WLRKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKLKEYGFYQGTEHRTIK
                     YLNNLIEQDHRPVKRRNKFYRSLRTASTTIKGMEA"
BASE COUNT          231 a          106 c          126 g          174 t
ORIGIN      
        1 tactatcaaa taaggtagaa taatagaaaa agatagcagg aggaatgacg atgaatcatt
       61 ttaaaggaaa gcaatttcag caggatgtga ttattgtagc cgtgggctac tatcttcgtt
      121 ataaccttag ctatcgtgaa gttcaagaaa tcttatatga tcgtggcatt aacgtttctc
      181 atacgacgat ttatcgttgg gtgcaagaat atggcaaact actctatcaa atttggaaaa
      241 agaaaaataa aaaatccttt tattcatgga aaatggatga aacgtacatc aaaattaaag
      301 gaaaatggca ttatttgtat cgagccatcg atgcagatgg tttaaccttg gatatttggt
      361 tacgtaaaaa acgggacaca caagcagcct atgcttttct taagcggtta gtgaagcagt
      421 ttgatgaacc gaaggttgta gtcacagata aagccccctc tattacaagt gcctttaaga
      481 aactaaaaga atacggcttt tatcaaggga cagaacatcg taccattaaa tacctgaata
      541 atttgattga acaagaccat cgtccagtaa agagacgcaa taaattctat cgaagtttac
      601 gcactgcctc taccacgatt aaaggcatgg aagccat
//
LOCUS       DAZKKW010000292          630 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.303, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000292 DAZKKW010000000
VERSION     DAZKKW010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..630
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.303"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..283
                     /locus_tag="ACG8PO_003055"
     CDS             <1..283
                     /locus_tag="ACG8PO_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFQ0208289.1"
                     /translation="LIQDDLHHTHRLWLMNKINLYFGHSYVFAFYDYRTNITEVDLVI
                     SNYYIDTGKTPLLLMKNIPTERNWRLFEKTIYQLKKEKEVVKSAFCPEY"
BASE COUNT          215 a           88 c          118 g          209 t
ORIGIN      
        1 tttaatacaa gatgatttac accatacgca tcggttatgg ttaatgaata aaattaatct
       61 gtattttggg cattcttatg tttttgcttt ttatgattac aggacaaata taactgaagt
      121 agatttggtt atttctaatt actacatcga cactggaaaa acgccattac tgttaatgaa
      181 aaatatacct accgaaagaa actggcgttt gttcgagaaa acgatttatc aattgaaaaa
      241 agaaaaagag gttgtgaaat cagcgttttg tcctgagtat tagagaaaca aacggaaaaa
      301 tagtttttca tattttgccg tttttgagct aataccgagg gacagctgat tgaacaccat
      361 ttattgagag attgtgaaaa aactgtcctg catcaagtaa taagaacagc caagtaaaaa
      421 cagcttctca tgtttgttga atattgtgac ttgtgaccga ggagttggca agagaacacc
      481 gtttattcgg aaaaagggac tgtgacacta catgtcacag tccctttttg attgaattat
      541 ctcatttgtt ttctaatttt ttctttattt gagaaaatag ttgaactgga taaaaagtac
      601 tggacgtgtc aaaattattt tggatcaaat
//
LOCUS       DAZKKW010000293          588 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.304, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000293 DAZKKW010000000
VERSION     DAZKKW010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..588
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.304"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(65..150)
                     /locus_tag="ACG8PO_003056"
     tRNA            complement(65..150)
                     /locus_tag="ACG8PO_003056"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(114..116),aa:Leu,seq:taa)
     gene            complement(167..238)
                     /locus_tag="ACG8PO_003057"
     tRNA            complement(167..238)
                     /locus_tag="ACG8PO_003057"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(204..206),aa:Gly,seq:gcc)
     gene            complement(250..322)
                     /locus_tag="ACG8PO_003058"
     tRNA            complement(250..322)
                     /locus_tag="ACG8PO_003058"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(287..289),aa:Thr,seq:tgt)
     gene            complement(340..421)
                     /locus_tag="ACG8PO_003059"
     tRNA            complement(340..421)
                     /locus_tag="ACG8PO_003059"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(385..387),aa:Leu,seq:tag)
     gene            complement(429..501)
                     /locus_tag="ACG8PO_003060"
     tRNA            complement(429..501)
                     /locus_tag="ACG8PO_003060"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(466..468),aa:Lys,seq:ttt)
     gene            complement(505..577)
                     /locus_tag="ACG8PO_003061"
     tRNA            complement(505..577)
                     /locus_tag="ACG8PO_003061"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(542..544),aa:Val,seq:tac)
BASE COUNT          149 a          169 c          138 g          132 t
ORIGIN      
        1 cccctaacct tttgatccgt agtcaaacac tctatccagt tgagctatgg gcgcatattt
       61 gaaatgccga ggaccggaat cgaaccggta cggtgatcac tcaccgcagg attttaagtc
      121 ctgtgcgtct gccagttccg ccaccccggc gcgattgctt tggcaaagcg gaaaacgggg
      181 ttcgaacccg cgacccccac cttggcaagg tggtgctcta ccactgagct atttccgcgt
      241 gtttaaagat gccggctaaa ggacttgaac cctcgaccct ctgattacaa atcagatgct
      301 ctaccaactg agctaagccg gcatgtaaaa cttaaattat gcgggtgaag ggacttgaac
      361 ccccacgccg taaggcgcta gatcctaaat ctagtgcgtc tgccaattcc gccacacccg
      421 caaaaatatg agccgtacag ggctcgaacc tgtgaccctc tgattaaaag tcagatgctc
      481 taccaactga gctaacggct catatggagg ttaacgggat cgaaccgctg accctctgct
      541 tgtaaggcag atgctctccc agctgagcta aacctccatg gataactt
//
LOCUS       DAZKKW010000294          565 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.306, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000294 DAZKKW010000000
VERSION     DAZKKW010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..565
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.306"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(26..508)
                     /locus_tag="ACG8PO_003062"
     CDS             complement(26..508)
                     /locus_tag="ACG8PO_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0208290.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     FVVSRNFPALIVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSMFSLLPRLIIGP
                     NGLSESGLKDLAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
BASE COUNT          214 a          112 c           82 g          157 t
ORIGIN      
        1 atgttttttt actgatgatc caccatcaat agttattttt tatagataca gatcgttcac
       61 tttttatcct gatttctgtc cagaagtaaa aaaatgttgc aagaataaat gaggcaatta
      121 ctaaatagaa ccatggtttg atcgtcaaga aatacatgga ggaataagct aaatccttca
      181 atccactctc gctcaaacca ttagggccga taatcaagcg aggcagcaaa gaaaacatag
      241 atgtaaatac taataagcaa gaaaagtaaa gcagctgcca attgtagtga ttgatcagag
      301 attttggtaa taagagacct acacttatca ctaaatagaa aacaatcaaa gctggaaaat
      361 tcctagaaac cacaaatccg cttccttgga tcaggcctaa tatctccgca ccttgtacaa
      421 cagcatttac ccatgtaaaa ggtagcaaaa cgataggaaa aaaccaaaaa aataaataaa
      481 gccgatcgat tactgctttt cttctcatta acgttccccc aaactacttt ttctctcatc
      541 atactataca attttctatc taaaa
//
LOCUS       DAZKKW010000295          565 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.307, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000295 DAZKKW010000000
VERSION     DAZKKW010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..565
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.307"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            141..539
                     /gene="spxA"
                     /locus_tag="ACG8PO_003063"
     CDS             141..539
                     /gene="spxA"
                     /locus_tag="ACG8PO_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SpxA"
                     /protein_id="HFQ0208291.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT          199 a           88 c          110 g          168 t
ORIGIN      
        1 taactttcat ttttttaaaa aaattatgaa aaaaaggtag ctatttaaaa gttgattatg
       61 tataatagaa acagatggga gattattatg atcttctgtt acaaaaaagt gattcttatc
      121 acgaaaagga gtggtgcacg ttgttaacat tatatacttc cccaagttgt acttcctgca
      181 gaaaggctcg tgcgtggttg caagaacatc agattccatt tgttgaacgt aatatttttt
      241 cagaaccatt gaacagttct gaattaaaag cgattttaca aatgacagaa gacggaacag
      301 aagaaatcat ctcgacacgt tccaaagtat tccaaaaact gaacatggat ttagatgatc
      361 tgccgttaca agaattattg gaacttgtgc aaaacaatcc tggattgctt cgccgtccaa
      421 tcatgattga tgataaacgt ctgcaagttg gttttaatga agatgaaatt cgtcggttcc
      481 taccgcgcga tgtacgacaa ttagaattac gtcaagcaca attgatggct ggattataga
      541 aggataatac gaaacttgga aaaga
//
LOCUS       DAZKKW010000296          557 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.308, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000296 DAZKKW010000000
VERSION     DAZKKW010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..557
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.308"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          192 a          101 c           62 g          202 t
ORIGIN      
        1 tggtactttt tattcggcaa tccattttat ttttctactt cttcttaata cctctaacac
       61 ttttgattta taaactaatc caatgcatct acttgcgctt attaagctat ttcagctaga
      121 tagtaccacc ccatgcgtca atgcttgatt ttccttttgt ataacattgt ataagcaata
      181 aaggacaaat aagtggtgat tacaccaaac cattgaataa gaaaaattct gatacgtaaa
      241 taaacatgaa cttgaaaaaa ttaatgaatc ggttagctac tacgtagttg tcttttttct
      301 catacagttc tgttaactta cacttctctt cgctagataa taaaaaatcc tccaactgta
      361 ttgtccaata taatcccagg catctaaact agcattatag aagagacaca catagaatct
      421 aaaataactg gttttaaatg tttcttacaa tcaaaaaaag ctgttaaatg cactccacac
      481 cttcatactc tatcattttt tataaacgat atatttttat tgtttttcga taaaaaatag
      541 ttgttttaca ataatta
//
LOCUS       DAZKKW010000297          554 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.309, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000297 DAZKKW010000000
VERSION     DAZKKW010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..554
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.309"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          196 a           60 c           93 g          205 t
ORIGIN      
        1 tttgtcaata ggaaaaattt atttctctaa aaaatataaa taatctgcag tgaaacttga
       61 gtacatatac taatttcgaa aaatgcctat gatttaaata attatggtgc tgggatgaag
      121 gtttataagt ttgtcttgat ttgtttagaa gaaatcgttt ttgcacacag ttgaattagg
      181 aaactactgg agtggaaaat aaagtgattt gacgggtaga tgggtgcact taaatttgaa
      241 aatggcttaa aatgctttat tttgaattgc agaatttctt ttagtacgca atttatggtc
      301 ttaaaattga ttaaaaagtg atacattgat tttaatacaa tttatgatac aaaaaatgac
      361 ctcctttctt aaataaaaga ggtcattttg ttttgtctca taatcgattg tttttgatac
      421 agtagttata ttcagaaaaa ataaagactc ttctgagttt ataatccttt ggattatata
      481 cggatacaga tgtagacata aaacaagctc aattttcaaa aatggcatta ctcacctttt
      541 tggtgaatta tttg
//
LOCUS       DAZKKW010000298          524 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.310, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000298 DAZKKW010000000
VERSION     DAZKKW010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..524
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.310"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            238..>524
                     /locus_tag="ACG8PO_003064"
     CDS             238..>524
                     /locus_tag="ACG8PO_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HFQ0208292.1"
                     /translation="MQDKLKELLAIRDGNLSMKEARKFGIAATTVQRLVNQGKLIKVD
                     RGYYVEDGHGIDDLFWLQQRFSRGIFSHETTLDIYQLSTNMPKFIHLTFPHG"
BASE COUNT          200 a           79 c          105 g          140 t
ORIGIN      
        1 aggggttatc ctatgcgaaa tacaacacac aaacaacaag aaatcgaaaa tcaaaaagca
       61 aaaattcgtt tagaacaaga gaaattacgt gcaaataaaa gaagactgca acaattaaag
      121 aaccagcagc gcattcaaaa agaaatcgaa ttagatcgat agacgttaat cttgttacaa
      181 ataaactact atgatatatt tgatgtagtg tatttgtaac gaagggaggg gaagtaaatg
      241 caagataaac tgaaagaatt gttggctatt cgcgatggaa atttatctat gaaggaagcc
      301 aggaaatttg gaatcgcagc aaccactgta caaagattgg tgaatcaagg aaaattgatc
      361 aaagttgatc gtggttatta tgtagaggat ggtcatggaa tagatgattt attttggctt
      421 cagcaacgat tttccagggg aatcttctct catgaaacga ctttagatat ttaccagctt
      481 tctacgaata tgccgaaatt cattcactta acttttcccc atgg
//
LOCUS       DAZKKW010000299          523 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.311, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000299 DAZKKW010000000
VERSION     DAZKKW010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..523
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.311"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..287)
                     /locus_tag="ACG8PO_003065"
     CDS             complement(<1..287)
                     /locus_tag="ACG8PO_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HFQ0208293.1"
                     /translation="MQEKLKELLAIRDGNLSMKEARKLGIAATTVQRLVNQGKLIKVD
                     RGYYVEDGHGIDDLFWLQQRFSRGIFSHETTLDIYQLSTNMPKFIHLTFPHG"
BASE COUNT          140 a          101 c           82 g          200 t
ORIGIN      
        1 ccatggggaa aagttaagtg aatgaatttc ggcatattcg tagaaagctg gtaaatatct
       61 aaagtcgttt catgagagaa gattcccctg gaaaaacgtt gctgaagcca aaataaatca
      121 tctattccat gaccatcctc tacataataa ccacgatcaa ctttgatcaa ttttccttga
      181 ttcaccaatc tttgtacagt ggttgctgcg attcctaatt ttctggcttc cttcatagat
      241 aaatttccat cgcgaatagc caacaattct ttcagtttct cttgcattta cttcccccct
      301 tttgttacaa atacactaca ttaaatatat catgatagtg tatttgtaac aaggttaatg
      361 tctatcgatc taattcgatt tctttttgaa tccgttgctg gttctttaat tgctgaagtc
      421 ttcttttact ggcacgtaat ttctcttgtt ctgaacgaat tttggctttt tgattttcga
      481 tttcttgttg tttgtgtgtt gtattacgca taagattaac cct
//
LOCUS       DAZKKW010000300          514 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.312, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000300 DAZKKW010000000
VERSION     DAZKKW010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..514
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.312"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..132)
                     /locus_tag="ACG8PO_003066"
     CDS             complement(<1..132)
                     /locus_tag="ACG8PO_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFQ0208294.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
BASE COUNT          186 a           94 c           92 g          142 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaatagcaa attcccgttg gatcctttcc atacatccat tgtactctga
      421 gaaatcacac aatatcgaaa caaattccgt taggaatgtg ttattcaata aattttataa
      481 tctagaaaca ttttgaataa aaactattag acaa
//
LOCUS       DAZKKW010000301          508 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.313, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000301 DAZKKW010000000
VERSION     DAZKKW010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..508
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.313"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(140..376)
                     /locus_tag="ACG8PO_003067"
     CDS             complement(140..376)
                     /locus_tag="ACG8PO_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0208295.1"
                     /translation="MKQNTSIAKNTTKFKCTAAAWRISISPTNRTNNRGMILFIWISF
                     FLLGYRVLPAYFAPPKRRGRLERLVRNKNHSLSY"
BASE COUNT          168 a           95 c          103 g          142 t
ORIGIN      
        1 acctatactt acgacgatac agaagaaatc caaaatgaac ttgaccgtaa acgaatgcca
       61 tatcaacgat ttgaaaggaa aaaaaggcaa taatcattta taccgaaaag tgagaatgtg
      121 tgaaataagg acaaaaaacc tagtaactaa gggaatggtt cttatttcgc accaatcgct
      181 ccaaccgccc ccgccgctta ggtggtgcga aataagcggg caacacccga taacctaaga
      241 gaaagaagga gatccatatg aataaaatca ttcctctatt gttagtccta tttgttggcg
      301 aaatacttat gcgccatgct gcagctgtgc atttaaactt cgtagtattt ttggcaatac
      361 tggtattttg tttgatcttt tggcgctttg tattagccct tgtgagtcta ctattcaaga
      421 tgattgcagc catatttttc atagggttat ttcgatgaaa ggagagagta ctaatgccaa
      481 ttgcaatagg aaataaacgg ttacccgt
//
LOCUS       DAZKKW010000302          503 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.314, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000302 DAZKKW010000000
VERSION     DAZKKW010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..503
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.314"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..375
                     /locus_tag="ACG8PO_003068"
     CDS             <1..375
                     /locus_tag="ACG8PO_003068"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0208296.1"
                     /translation="DDNMVFDIDNLKGFLNDTSSFGFIAKENNKIIGFAYCYTLLRPD
                     GKTMFYLHSIGMLPNYQDKGYGSKLLSFIKEYSKEIGCSEMFLITDKGNPRACHVYEK
                     LGGKNDYKDEIVYVYDYEKGDK"
BASE COUNT          201 a           35 c           86 g          181 t
ORIGIN      
        1 gatgataata tggtttttga tattgataat ttaaaaggat ttcttaatga taccagttca
       61 tttgggttta tagctaaaga aaataataaa attataggat ttgcatattg ctatacactt
      121 ttaagacctg atggaaaaac aatgttttat ttacactcaa taggaatgtt acctaactat
      181 caagacaaag gttatggttc aaaattatta tcttttatta aggaatattc taaagagatt
      241 ggttgttctg aaatgttttt aataactgat aaaggtaatc ctagagcttg ccatgtatat
      301 gaaaaattag gtggtaaaaa tgattataaa gatgaaatag tatatgtata tgattatgaa
      361 aaaggtgata aataaatgaa tatagttgaa aatgaaatat gtataagaac tttaatagat
      421 gatgattttc ctttgatgtt aaaatggtta actgatgaaa gagtattaga attttatgat
      481 ggtagagata aaaaatatac att
//
LOCUS       DAZKKW010000303          498 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.315, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000303 DAZKKW010000000
VERSION     DAZKKW010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..498
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.315"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            258..498
                     /locus_tag="ACG8PO_003069"
                     /pseudo
     CDS             258..498
                     /locus_tag="ACG8PO_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611995.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
BASE COUNT          169 a           91 c           69 g          169 t
ORIGIN      
        1 ttttacactc tctattattc ttttcataaa tattgtaaat agaagtatta aaaatatata
       61 caaaaagtat tatttcgaga aagataaata ttttcaaaaa ccttttcata atcctcaccc
      121 tttcttacac tgataaatca tgcgtctaat attcaaatta tactcgatat gctttagcta
      181 atcagccaac tctgtttgtc tactatcatt atatcaatag acaatatctg ctcccgtctt
      241 tatgatcgtg ccaaagcaat caaaagaaaa ttgcgaatca cttagatcga aagtttactc
      301 aaacaaaagc attgtcagcg gttgtcacgg atttaactta tgttcgggtc gggacaaatt
      361 gggcttatat ttgtttgatg attgatttat tcaatcgtga aattattggc cattcttgcg
      421 gaaataaaaa agatgcgcag cttgtaaaaa gggccattca aagcattcct tactcacttc
      481 aagaaatcga actcttcc
//
LOCUS       DAZKKW010000304          488 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.316, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000304 DAZKKW010000000
VERSION     DAZKKW010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..488
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.316"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..86
                     /locus_tag="ACG8PO_003070"
     CDS             <1..86
                     /locus_tag="ACG8PO_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFQ0208297.1"
                     /translation="GKVLGITRQGATNLKHRLYKKIQKMLV"
     gene            180..371
                     /locus_tag="ACG8PO_003071"
     CDS             180..371
                     /locus_tag="ACG8PO_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0208298.1"
                     /translation="MNSLKEDFILAKAGNEEAVEAILKRFSSLIHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
BASE COUNT          181 a           67 c           84 g          156 t
ORIGIN      
        1 ttggaaaggt tctggggatt acaagacagg gtgcgacaaa tttaaaacat cgactttaca
       61 aaaaaattca aaaaatgctg gtttaatatc tgcataataa ctaattttgt atgcttcttt
      121 gctcgttctc ctccttccca aagaaataga ttataagtcc atgggaagaa ggtagattta
      181 tgaattcttt aaaagaagat ttcattttag caaaggctgg aaatgaagaa gctgttgagg
      241 caattctcaa aagatttagc tctttgattc ataagcaatc ctggagaact ggaaaatatg
      301 atcaagattg ttatcaagaa tgtatgcttg caatatatct agctatctcg aaattcgaaa
      361 ttaaagaata actcaactaa gggagtctgt gacataagta aaaagatagt ttcaaatttc
      421 gaacaataaa tttgaaacta tcttctttat aattataaag tatcgatttt acagcgttct
      481 ataatgat
//
LOCUS       DAZKKW010000305          486 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.317, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000305 DAZKKW010000000
VERSION     DAZKKW010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..486
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.317"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>486
                     /locus_tag="ACG8PO_003072"
     CDS             <1..>486
                     /locus_tag="ACG8PO_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFQ0208299.1"
                     /translation="CFPNAQVIVDRFHIVQHLNRFFDAVRKRVMKTLDQKDPIQAKQY
                     RQLKSLFKLLLKSEDRLDYNTLTKRRNFNWRLLTETEVVDRLLKISPELKTAYRYYQD
                     LLTAYQEKDPDTFFQLLRAMPGEVPLELHHIKKAFFKYEKGIRLALLKKYSNARLENL
                     H"
BASE COUNT          165 a          102 c           87 g          132 t
ORIGIN      
        1 agtgttttcc aaatgcacaa gtgatcgtcg atcggtttca cattgttcaa catctgaacc
       61 gcttcttcga tgcggttcga aaacgtgtga tgaaaacact tgatcaaaaa gacccaatac
      121 aagctaaaca gtatcgccaa ttgaaatcct tattcaaatt acttttaaaa tcagaggatc
      181 gtttagatta caatacacta acgaaaagac gcaatttcaa ttggagacta ttgactgaaa
      241 cagaagtagt tgaccgcctt ctaaagattt ctcctgaact taaaacagct tatcgttact
      301 accaagacct gcttactgcg tatcaggaaa aagatccaga tacttttttc cagctgcttc
      361 gagcaatgcc tggagaagtt ccattggaac tgcaccatat caaaaaagcc tttttcaaat
      421 acgagaaagg gattcggctg gcattactga agaagtattc gaatgcacga ctggaaaatc
      481 tccata
//
LOCUS       DAZKKW010000306          478 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.318, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000306 DAZKKW010000000
VERSION     DAZKKW010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..478
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.318"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..270
                     /locus_tag="ACG8PO_003073"
     CDS             <1..270
                     /locus_tag="ACG8PO_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HFQ0208300.1"
                     /translation="ELVKLEMAETGAGVRSSRHWKAAANIEFYYNEIQKGFDQMRELD
                     RQTNWSKKLHQDRFKFVEKYKEILEEYLRRTANDKKTRSIQHGFI"
     gene            230..475
                     /locus_tag="ACG8PO_003074"
     CDS             230..475
                     /locus_tag="ACG8PO_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0208301.1"
                     /translation="MIKKLVQFSMDLYDIESGATLSVESDHLIINFGGKRQIILWVVD
                     DVLFPEIVHDFEESKAVEFEIVKKVMELIEKYEEDSE"
BASE COUNT          181 a           58 c          116 g          123 t
ORIGIN      
        1 gaattagtta agttagaaat ggctgaaaca ggagcaggag tgcgaagtag caggcattgg
       61 aaagcagcag caaacattga attttattac aacgaaattc aaaaagggtt cgatcagatg
      121 cgtgagctgg atcggcaaac aaattggagc aagaaacttc atcaagatcg tttcaaattt
      181 gtagagaagt ataaagaaat attagaagag tatttgagga ggacagcaaa tgataaaaaa
      241 actcgttcaa ttcagcatgg atttatatga tatcgaatca ggagcaacac tatctgtgga
      301 atcggaccat ctaatcataa attttggtgg aaagcgccag attattttgt gggtagttga
      361 tgatgtactg tttccagaaa ttgttcatga tttcgaagaa tcaaaagcgg ttgagtttga
      421 aatagtgaaa aaagtaatgg aattgattga aaaatacgag gaggacagcg aatgatac
//
LOCUS       DAZKKW010000307          459 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.320, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000307 DAZKKW010000000
VERSION     DAZKKW010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..459
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.320"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>459
                     /locus_tag="ACG8PO_003075"
     CDS             <1..>459
                     /locus_tag="ACG8PO_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HFQ0208302.1"
                     /translation="KRGIQISRRKISRIMKNRGLKSSYTVAYFKVHHSTCNEAKTTNV
                     LNRKFLRDNPLEAIVTDLTYVRVGKKWNYVCFILDLFNREILGYSCGEHKDAVLVKKA
                     FSRIKQPLTEVEIFHTDRGKEFDNQAIDELLTTFDINRSLSHKGCPFDNAV"
BASE COUNT          163 a           75 c           87 g          134 t
ORIGIN      
        1 aaacgaggca ttcagatcag ccgacgaaaa attagtagaa tcatgaaaaa tagaggatta
       61 aaatcgagct atactgttgc ttattttaaa gtacatcatt ctacttgcaa tgaagccaaa
      121 acgacaaacg tattgaatcg taaattctta agagacaacc cattagaagc gatcgtaaca
      181 gacttgactt atgtacgagt cgggaaaaaa tggaattatg tctgtttcat tttggatctg
      241 ttcaatcgag aaattctcgg ctattcttgt ggagaacata aagatgccgt tctagtaaaa
      301 aaagcattta gccgtatcaa acaacctctg acagaggttg agatttttca tactgatcgt
      361 ggaaaagagt ttgataacca agctattgat gaattattaa caacttttga catcaatcga
      421 tcattgagtc ataaaggctg tccttttgat aatgccgta
//
LOCUS       DAZKKW010000308          451 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.321, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000308 DAZKKW010000000
VERSION     DAZKKW010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..451
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.321"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>451
                     /locus_tag="ACG8PO_003076"
     CDS             <1..>451
                     /locus_tag="ACG8PO_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFQ0208303.1"
                     /translation="FRKGITMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKVR
                     TLNDKYVAIFMDATYIPLKRQTVSKEAIYIAIGIREDGTKEVLSYAIAPTESTYVWNE
                     LLQDIYSRGVHDVLLFITDGLKGMKDTIHQIYPKAKYQHCCVHVSRN"
BASE COUNT          147 a           89 c           87 g          128 t
ORIGIN      
        1 tcttccgaaa aggtattacc atgtcggaaa tcgcagactt gattgagaaa atgtatgggc
       61 atcactacac gccccaaacc atgtccaaca tgactaaagt gctgactgaa gaagtaaatg
      121 catttaaagt tagaactcta aatgataagt atgtcgctat tttcatggat gctacttata
      181 ttccattaaa acggcaaacc gtctccaaag aagcaatcta tattgccatt ggtatacgag
      241 aagacggcac taaagaagta ctaagttatg cgattgctcc gactgaatcg acctacgttt
      301 ggaatgagtt gttacaggac atttactcca gaggggttca tgatgtctta ctgtttatta
      361 ccgatggctt aaaaggcatg aaagatacga ttcaccagat ttatcctaaa gctaaatacc
      421 aacattgctg tgttcacgtt tcccgtaata t
//
LOCUS       DAZKKW010000309          447 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.322, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000309 DAZKKW010000000
VERSION     DAZKKW010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..447
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.322"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>447
                     /locus_tag="ACG8PO_003077"
     CDS             <1..>447
                     /locus_tag="ACG8PO_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFQ0208304.1"
                     /translation="HLEKAINTLLRTELTAFLDYEKYDRTGFNSGNSRNGSYFRSIKT
                     EYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLETTIIHLFEKGVTMSEIADLIEKMYG
                     HHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAIFMDATYIPLKRQT"
BASE COUNT          165 a           85 c           72 g          125 t
ORIGIN      
        1 gcacttagaa aaagcgataa acacactcct acggactgaa ttaacggctt ttttagatta
       61 cgaaaaatat gatcgcactg gttttaattc aggtaattcg agaaacggtt cttactttcg
      121 atcaatcaaa accgaatatg gtgaattaac attggaaata cctagagatc gtaatggtga
      181 gtttaaacaa caaactttac cagcctacaa aagaacaaac gatacattgg aaaccactat
      241 tatccattta ttcgaaaaag gtgttacgat gtctgaaatt gctgatttga tcgaaaaaat
      301 gtacggtcat cactatactc cacaaaccat gtccaacatg actaaagttc tgactgaaga
      361 agtaaatgcc tttaaatcca gagccttaaa tgataagtat gtcgctattt ttatggacgc
      421 tacttacatt ccactaaaac gtcaaac
//
LOCUS       DAZKKW010000310          443 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.323, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000310 DAZKKW010000000
VERSION     DAZKKW010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..443
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.323"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>443
                     /locus_tag="ACG8PO_003078"
     CDS             <1..>443
                     /locus_tag="ACG8PO_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFQ0208305.1"
                     /translation="ITQTQLKPEDYPKIFPNRVFDFQLDKKGQSELQAGIQAVANNIG
                     IQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNTRNTPTQHLATSIHELAHARM
                     HKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYIAAWTKNG"
BASE COUNT          162 a           80 c           91 g          110 t
ORIGIN      
        1 atcacacaaa cacagttaaa acctgaagac tatccgaaaa tctttccgaa ccgtgtcttt
       61 gattttcaat tggataaaaa aggacagtca gaacttcaag ctgggattca agcggtagcc
      121 aacaatattg gaatacaaat tcgagatatg acagaaagtg aagtctatca aagagaatta
      181 ggacaagcac gtggggcgta tgttcgtaac gaatttactg gcgaagaaga aatcgtcatg
      241 aatactcgaa atactcctac gcagcattta gctacaagta ttcatgaatt agcccatgca
      301 agaatgcaca aattttcaga attagatacc gcaactaaag aattgcaggc agaaatgact
      361 tcttatattg tgtgcaaaca ttttggaatg gacacgtcgg agaaagctat tccttatatt
      421 gcagcatgga cgaagaatgg tca
//
LOCUS       DAZKKW010000311          443 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.324, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000311 DAZKKW010000000
VERSION     DAZKKW010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..443
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.324"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          179 a           53 c           79 g          132 t
ORIGIN      
        1 attctagagt taaaagtaac tattcgaaaa ggataaggtt agaatttttt gttttgccag
       61 tggaagaata taaaaagggc attggaaatt aatatattag aatgctaagc ggagaaaaaa
      121 atatatgaaa agtacgtcct tgtgatggga tggaaaaata cacttatttc aaacaacgat
      181 aatataatta tgtgaaccga agtatactcg cttctaattc aatcctcaaa tcccgacagt
      241 cgcgacttca aaaaagcgta tagttttaat gtatccccat atgatccgaa aaatggtaat
      301 ttacaattaa cagtttgatg atgttgaaga tgtggaaatg ctgaaattac catgatgaga
      361 aacacattaa ttaaagggaa ttaattaact agaatataat ctgattcaat ttgaattaag
      421 caaaaaaagg ctgagtttta aat
//
LOCUS       DAZKKW010000312          440 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.325, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000312 DAZKKW010000000
VERSION     DAZKKW010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..440
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.325"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            11..>440
                     /locus_tag="ACG8PO_003079"
     CDS             11..>440
                     /locus_tag="ACG8PO_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFQ0208306.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRA"
BASE COUNT          168 a           57 c           88 g          127 t
ORIGIN      
        1 ggtgcttttt ttgcggaaat atacatttgt ttttaagaaa aaagtagttt cagactattt
       61 aaataacgaa ggcggctaca aatatcttgc acataaatat caaataaatc gtacactggt
      121 tagacattgg gtaaggattt ataactatca tggttgggaa ggcttggttg gaggtggcaa
      181 aagctacact acaaaattta aacttgatgt tatagaatat atggaaacaa atggtctttc
      241 tatccaagaa actgctaaaa aatttaatat cggttcaaat agaactctaa gtaaatggat
      301 agagcaatat gaagaaggcg gtgcttcttc acttgagagc caaaaaaggg gcagaaaaat
      361 tagtatgaat tccaagctaa acattcctaa aaaacttaaa gatgagtctc ttgaagaaga
      421 agttattcgt ttaagagcag
//
LOCUS       DAZKKW010000313          433 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.327, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000313 DAZKKW010000000
VERSION     DAZKKW010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..433
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.327"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>433)
                     /locus_tag="ACG8PO_003080"
     CDS             complement(<1..>433)
                     /locus_tag="ACG8PO_003080"
                     /inference="COORDINATES: protein motif:HMM:NF014376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HFQ0208307.1"
                     /translation="IMEDFWYHNKSVSAPISLSQCGYESYHPNSSIRNYIVQQKWIFH
                     YVLSGKGFLEVETQHFELKEHDIFFFFQGQKVKYYTDKKEPWTLIWLGIQGDKTSEFL
                     KETTLLNTHTVSLTKNMNKKIKNILLKIVCENRELIDEPETL"
BASE COUNT          145 a           66 c           57 g          165 t
ORIGIN      
        1 taaagtttcc ggttcatcaa ttagttctct attttcacat actattttca atagtatgtt
       61 ttttattttt ttattcatat tcttagtcaa gctaactgta tgagtattta gtaaagttgt
      121 ttctttcaaa aattcagaag tcttatcacc ttgtatacct aaccaaatca gtgtccaagg
      181 ttccttttta tctgtataat acttcacttt ttgaccttga aaaaaaaaga aaatatcgtg
      241 ttctttaagt tcaaaatgtt gagtttctac ttctaagaat cccttaccag ataaaacata
      301 atgaaatatc catttttgtt gaactatata attacgaata gaagaattgg gatgataaga
      361 ttcatatccg cattgggaaa gggaaattgg agcagaaact gacttattat gataccaaaa
      421 atcctccatt att
//
LOCUS       DAZKKW010000314          426 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.328, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000314 DAZKKW010000000
VERSION     DAZKKW010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..426
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.328"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>426
                     /locus_tag="ACG8PO_003081"
     CDS             <1..>426
                     /locus_tag="ACG8PO_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="UTRA domain-containing protein"
                     /protein_id="HFQ0208308.1"
                     /translation="KSNGFTPTSIVLERKIIPATEGMAKKLDIAQGDDVLYIQRKRMA
                     DNIPIMLENNYFSYERYSFLMEEELTDSLYEVLKVQGTLAINPGETALEIVTADDQIA
                     KIMEVAVGTPFFFMDTLINDQNNIPIHVGNQYYLGEVYR"
BASE COUNT          143 a           73 c           84 g          126 t
ORIGIN      
        1 gtaaatctaa tggttttaca ccgactagca ttgttttgga acgcaagatc attccagcaa
       61 ctgaagggat ggcaaaaaaa ctcgatatcg ctcaaggtga tgatgtttta tatatccaga
      121 gaaagcgaat ggcggataac atccccatta tgttagaaaa caattatttt tcttatgaaa
      181 gatatagttt cttaatggag gaagagctga ctgattcttt gtatgaagta cttaaagtcc
      241 aaggtacctt agccatcaat cctggggaaa ctgcacttga aattgtcaca gctgatgacc
      301 aaattgccaa aataatggaa gtagctgttg ggacgccctt tttctttatg gatactttaa
      361 tcaacgatca aaacaacata ccaattcatg taggtaatca atattacctg ggagaagtat
      421 atcgtt
//
LOCUS       DAZKKW010000315          423 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.329, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000315 DAZKKW010000000
VERSION     DAZKKW010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..423
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.329"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          146 a           60 c           70 g          147 t
ORIGIN      
        1 aaaatattat gataaaaatc acaaacgtag ccttttacgt cataagtgtt aaaagaaata
       61 attaatttca aatatagaaa agggaacgta tgtttgcata ctcctttttt ttgagttata
      121 ataatacgcg aagagagata atttcattat ttaaacaagc taaaaatctt gatattactt
      181 cttttcaggc aatacgaata ttattcgctt acattttttg tataaattta attgtctgct
      241 atgcttgtga tgaattgatt ttcgttttgc acttttggat gataaatttg gacaattgaa
      301 aatacaaaaa aaagaaaggc ggtatgaact cggaaggctg gtaaccttcg tcagcgttca
      361 tacacggcat taagatttgg cccttaatgc gttaaccttt cagtgttacg gtattacccg
      421 caa
//
LOCUS       DAZKKW010000316          403 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.331, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000316 DAZKKW010000000
VERSION     DAZKKW010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..403
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.331"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          163 a           52 c           64 g          124 t
ORIGIN      
        1 gatgaaagcg catactttta agaggggttg tatgtaattt taatacctat accaatttaa
       61 agaggatttt tcagattttc acttacaaat ttataaaaaa acaatataat tgatactttg
      121 attttgcgaa cattttgcct ttccgcctaa ttcagcttta ataccttgat tatatcagta
      181 actaatcgcc tttttagcta atttaagcat caaataatta tccttaatca gggtaaaaaa
      241 taacgaataa atggattttt ggaataggta aaaactagat cggtttctga cgaaaaataa
      301 cattaaaaag gaaatataaa ataacatgga gcgtgggagg aataaagaaa ttttacacac
      361 aaggggcgta ggaatgtaaa gaaacttaac acaaaacaaa agt
//
LOCUS       DAZKKW010000317          399 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.332, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000317 DAZKKW010000000
VERSION     DAZKKW010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..399
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.332"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            5..>399
                     /locus_tag="ACG8PO_003082"
     CDS             5..>399
                     /locus_tag="ACG8PO_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0208309.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQRKTNGQ
                     PMNDKRNGHSWFRQQERLENKVHSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIDNIPRIKEEIERFEKGESTFSAATIRK"
BASE COUNT          176 a           62 c           91 g           70 t
ORIGIN      
        1 ggacatggca gaagaaaaag aaccactaga caatagtcgc ttgggaaaat ccaaaagaaa
       61 attagtacga ttgcaaaatg aattaaacga acaaattgag aagatgtttg aacaccagag
      121 aaagaccaac ggtcagccaa tgaacgataa gagaaatggg cattcttggt ttaggcaaca
      181 agaacgttta gaaaacaaag tgcatagtct gagagaagag atcaagcaac aagaaaaaca
      241 ggtagaaaaa cttgaaagac aagaagagct aaaggaaatg ggctataaca agtacggcgg
      301 actggatatg acgatcgata atattccaag aatcaaagaa gaaatcgaac gctttgaaaa
      361 aggtgaatcg actttttcag ccgccacgat ccgaaaata
//
LOCUS       DAZKKW010000318          398 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.333, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000318 DAZKKW010000000
VERSION     DAZKKW010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..398
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.333"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            4..>398
                     /locus_tag="ACG8PO_003083"
     CDS             4..>398
                     /locus_tag="ACG8PO_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0208310.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQKMTNGQ
                     PMNDKRNGRSWFNQQERIENKVQSLREEIKQQEEYVEKLERQEELKEMGYNKYGGLDM
                     TIDNIPRIKEEIERFEKGESAFSAATIRK"
BASE COUNT          177 a           65 c           88 g           68 t
ORIGIN      
        1 aacatggcag aagaaaaaga accactagac aatagtcgtt tgggaaaatc caaaagaaaa
       61 ctagtacgat tgcaaaatga attaaacgaa caaattgaaa agatgtttga acaccaaaaa
      121 atgacgaacg gtcaaccaat gaacgacaag aggaatggcc gttcatggtt taaccagcaa
      181 gaacgaatcg aaaataaagt ccaaagtctg agagaagaga tcaagcaaca agaagaatac
      241 gtggaaaagc ttgaaagaca agaagagcta aaggaaatgg gctataacaa gtacggcgga
      301 ctggatatga cgatcgataa tattccaaga atcaaagaag aaatcgaacg ctttgaaaaa
      361 ggtgaatcgg ctttttcagc cgccacgatc cgaaaata
//
LOCUS       DAZKKW010000319          397 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.334, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000319 DAZKKW010000000
VERSION     DAZKKW010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..397
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.334"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..387
                     /locus_tag="ACG8PO_003084"
     CDS             <1..387
                     /locus_tag="ACG8PO_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFQ0208311.1"
                     /translation="DRKEICDDFKAVYQANSKEEANTFLSGMIEKWKKNYPKVTQSLI
                     ENQDLLTFYDFPPSIRRTIYSTNLIESFNKQIKRYSRRKEQFQNEESLERFLVSIFDT
                     YNQKFLNRSHKGFQQVTDTLVSMFTE"
BASE COUNT          153 a           72 c           60 g          112 t
ORIGIN      
        1 gaccgaaaag aaatctgtga tgactttaag gctgtttatc aagctaactc aaaagaagaa
       61 gcgaatacct tcttatccgg catgattgag aaatggaaga aaaactatcc taaagtgacg
      121 cagtcactca tagaaaacca agacttatta actttttatg attttccacc tagcattcgt
      181 agaaccattt actcaaccaa tctaatcgag tctttcaata agcaaattaa aagatacagc
      241 cgtagaaaag agcagtttca aaatgaagaa tcactagaac gctttctagt cagcattttt
      301 gatacataca atcaaaaatt tctaaacaga agccataaag gttttcaaca ggtaaccgat
      361 acattagttt caatgtttac tgagtaacta attattt
//
LOCUS       DAZKKW010000320          390 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.335, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000320 DAZKKW010000000
VERSION     DAZKKW010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..390
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.335"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            102..302
                     /locus_tag="ACG8PO_003085"
     CDS             102..302
                     /locus_tag="ACG8PO_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFQ0208312.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
BASE COUNT          134 a           67 c           75 g          114 t
ORIGIN      
        1 ttcggtaatc tgtggtagct tgtttcaaat aaacatcttc cagatataca ccattcaagt
       61 aattgcaaaa tatgtgttga tacacaagga ggatttttat tatggaaaca ggtacagtaa
      121 aatggtttaa ctcagaaaaa ggatttggat tcatcactgc agaaaacgga aacgatgtat
      181 tcgttcattt ctcagctatc caaggcgacg gattcaaaac tttagaagaa ggccaagcag
      241 taactttcga tgttgaagaa ggccaacgcg gacctcaagc aacaaacgtt actaaagctt
      301 aattaaccta tactaaaaaa taaacgccca gacatttgcg tgtctgggcg tttttgtcta
      361 ttttctttaa aaagcgatga gagtattttt
//
LOCUS       DAZKKW010000321          383 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.336, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000321 DAZKKW010000000
VERSION     DAZKKW010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..383
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.336"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          143 a           58 c           87 g           95 t
ORIGIN      
        1 ttaaatagtg atatttagta atatacgaat tataagatat attttatata gaatgtatgt
       61 aaataaataa aatgtctgac ggatattgaa tagcaataaa taacaataat tcagacggat
      121 acagtgagtg aagtagcttg ctacgaacga acaggcagaa tgaaacggca gtttcattag
      181 agcaagtcgc ttgcgacgta gcgttaccgc tgatgtaact ggcaagttac atagagagag
      241 tttgcttgca aacgaacgtt acctacttga atagaaataa aaaataacag attagtgaat
      301 tttgaatagg agccacaact caaccaaccg cttgcggtgg gtgagtccca cggaggaaaa
      361 gacgaacgaa cggtcgcgta gcg
//
LOCUS       DAZKKW010000322          360 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.338, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000322 DAZKKW010000000
VERSION     DAZKKW010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..360
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.338"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>360)
                     /locus_tag="ACG8PO_003086"
     CDS             complement(<1..>360)
                     /locus_tag="ACG8PO_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0208313.1"
                     /translation="EAYTTKGMDYFLYDYSPIASLENKEHIIKHEAKIGSQWVNLPYK
                     TTGSSIIYRVTNEKSQVITLPVYAYLGTNVEINHQPVKEQVNANGLVQISGVKGVADI
                     KVSTSYTVTTIISYLVTL"
BASE COUNT          106 a           69 c           52 g          133 t
ORIGIN      
        1 taatgtaaca agataagaaa taatagtcgt tactgtataa cttgtgctta ctttaatatc
       61 agcaactcct ttgactccgc ttatttgtac caatccatta gcattcacct gctcctttac
      121 aggttgatga tttatttcta cattcgttcc taaataagca tatacaggaa gtgtgattac
      181 ttggctcttc tcattagtta ctctatatat gatagaacta ccagttgttt tataaggaag
      241 gtttacccat tgactgccaa tctttgcttc gtgttttatt atatgctcct tattctccaa
      301 tgaagcgata ggagaataat catataaaaa ataatccatt cctttagtgg tatacgcctc
//
LOCUS       DAZKKW010000323          341 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.340, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000323 DAZKKW010000000
VERSION     DAZKKW010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..341
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.340"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>341
                     /locus_tag="ACG8PO_003087"
     CDS             <1..>341
                     /locus_tag="ACG8PO_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rib/alpha-like domain-containing protein"
                     /protein_id="HFQ0208314.1"
                     /translation="EATQPITADDTEFVSSATKLDDKSDADKYNPEGQKVTTELNKEP
                     DAFEGIKNKEDLPKDTKYTWKEKVDVSAAGNKKGTVVVTYPDGSSDEVEVDVTVTDNR
                     SDADKYEPTVEG"
BASE COUNT          141 a           50 c           76 g           74 t
ORIGIN      
        1 gaagcaactc aaccaattac agcagatgat acagaatttg tatcaagtgc aacaaaactg
       61 gatgataaat ctgacgctga caaatataat cctgaaggtc aaaaagtgac tacagaattg
      121 aataaggaac cagacgcatt tgagggaatt aagaacaaag aagatttacc aaaagatact
      181 aagtatactt ggaaagaaaa agtagatgtt agcgcagctg gaaataaaaa agggactgtt
      241 gtagtgacat atccagatgg atcatctgat gaagttgaag tagatgttac agtgacagac
      301 aatcgttctg acgccgataa atatgagcca acagtagaag g
//
LOCUS       DAZKKW010000324          339 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.341, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000324 DAZKKW010000000
VERSION     DAZKKW010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..339
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.341"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            19..>339
                     /gene="traE"
                     /locus_tag="ACG8PO_003088"
     CDS             19..>339
                     /gene="traE"
                     /locus_tag="ACG8PO_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraE"
                     /protein_id="HFQ0208315.1"
                     /translation="MSYTGILSLEDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGR"
BASE COUNT          130 a           51 c           70 g           88 t
ORIGIN      
        1 aagtgggtgg attattttat gtcatataca ggaattttga gtctcgaaga tatttgtcac
       61 tacggtaaac ggtgtaccgc tacagaaaag atcacgaaga aactatcaac tggtcaaagt
      121 aaaaccgttg tgcagtgtaa gaaatatata atccagaaag acaaagtctc agaagaaatg
      181 atttattaca ttgggaaaca aaaacaaata attttaaaag atcctatccc tttaaaagag
      241 ctatatccaa caatcaaaca tgtttatgac caaaacggtg tactgattgg tcgaagaaaa
      301 aatggagtct tgcgttgtac cgcaaaaggg atggggcgt
//
LOCUS       DAZKKW010000325          312 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.342, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000325 DAZKKW010000000
VERSION     DAZKKW010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..312
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.342"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>312
                     /locus_tag="ACG8PO_003089"
     CDS             <1..>312
                     /locus_tag="ACG8PO_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFQ0208316.1"
                     /translation="GDTIVGIHHKSAVITLVERLSKVIITIKPNGRKALDIETALNQW
                     FSRFPKNFFKSITFDCGKEFSNWKAISNQHDIDIYFADPGTPSQRPLNENSNGILRRN
                     GL"
BASE COUNT           97 a           63 c           55 g           97 t
ORIGIN      
        1 ggtgatacga tcgttggcat tcatcataaa agtgccgtca ttactttagt tgaaagatta
       61 tctaaagtca ttatcacgat taaacccaac ggccgtaagg cattagatat tgaaactgcc
      121 cttaatcaat ggttttctcg cttccctaaa aacttcttta aatctattac gtttgactgt
      181 ggaaaagaat tttctaactg gaaagccatt agtaaccaac atgatattga tatatatttt
      241 gcggaccctg gaacaccttc tcaacgccca ttaaacgaga attctaacgg gattctgcgt
      301 cgtaatggac tg
//
LOCUS       DAZKKW010000326          312 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.343, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000326 DAZKKW010000000
VERSION     DAZKKW010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..312
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.343"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(89..>312)
                     /locus_tag="ACG8PO_003090"
     CDS             complement(89..>312)
                     /locus_tag="ACG8PO_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFQ0208317.1"
                     /translation="FEDAFQYTVQGNSHNRLKSTNLIERLNQEVRRREKIIRIFPNQT
                     SANRLIGAVLMDLHDEWIYSSRKYINFDK"
BASE COUNT           96 a           47 c           58 g          111 t
ORIGIN      
        1 taaatagttt aaaaaaatga aaaataataa aggaagtgag tcaattccag actcctgtgt
       61 aaaatgctat acaatgtttt taccatttct acttatcaaa attgatgtat tttcttgaag
      121 aataaatcca ttcatcatgt aggtccataa gaacggctcc aattaagcga ttggctgatg
      181 tttgattggg gaagatgcga ataatctttt ctcttctgcg tacttcttga ttcagtcgtt
      241 caattagatt ggtactcttt agtcgattgt gggaatttcc ttgtacggta tattgaaagg
      301 cgtcttcgaa tc
//
LOCUS       DAZKKW010000327          305 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.348, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000327 DAZKKW010000000
VERSION     DAZKKW010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..305
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.348"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          110 a           50 c           37 g          108 t
ORIGIN      
        1 ataaaaaaag aagcataggt atgctatcac cctgcttctt cttatgattt gtatgatcaa
       61 tttattctat cactttacta attaatttga aaatctaata aaaactctca ctctaaaaga
      121 gtgagagact gcaaattaga gtacggactt ttttggagtt ccgttcttta atattaacaa
      181 atgaataaaa tccttacaac atttgagaat aagttatcag tagacactta caaaaaattc
      241 tcatactatt tcctccatca ttagtttttg tctatataag gtttacccta aatagacact
      301 ggtat
//
LOCUS       DAZKKW010000328          297 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.349, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000328 DAZKKW010000000
VERSION     DAZKKW010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..297
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.349"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          105 a           52 c           43 g           97 t
ORIGIN      
        1 ctctataatt tctaggactg atggaaattt tctatctgtc ctagtttttt tgtaaaaaaa
       61 caaaaagaca tacaaaattt ccggtagaat agagtctcct acaacatcat tctgggggaa
      121 ttcttgtatg tccatgaatc attttatcaa acaactcttt actattaaag actcaaatat
      181 ccaaattcaa tcctttgaag aaacttttta taaaagcaga aaaagtatcg ttattgaggg
      241 aactctgatc cgtacctaca gacgttgtcc gtgttgtaaa gattctacaa agcaaat
//
LOCUS       DAZKKW010000329          292 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.351, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000329 DAZKKW010000000
VERSION     DAZKKW010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..292
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.351"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..185
                     /locus_tag="ACG8PO_003091"
     CDS             <1..185
                     /locus_tag="ACG8PO_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFQ0208318.1"
                     /translation="YTFETLQQLDLELFDYVNWWNHLRLHGTLGYETPVGYRNQRLAQ
                     RILDNELGCANASEAV"
BASE COUNT           83 a           63 c           69 g           77 t
ORIGIN      
        1 aatacacatt tgaaacctta caacaattgg atttggagtt atttgactat gtcaattggt
       61 ggaaccacct tcggttgcac ggtacacttg gctacgagac accggttggt taccgtaacc
      121 agagattggc gcagcgaatc cttgataatg agctcggatg tgctaacgct agcgaggcag
      181 tctaacttta actgttagct cctgccgaag atcgtcacat ccgaggaggc tcattgtcaa
      241 ggacaatcgg aatagcatac ggaaagaagt tctgcacctt ataaaatttg tc
//
LOCUS       DAZKKW010000330          277 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.353, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000330 DAZKKW010000000
VERSION     DAZKKW010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..277
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.353"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..198
                     /locus_tag="ACG8PO_003092"
     CDS             <1..198
                     /locus_tag="ACG8PO_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0208319.1"
                     /translation="MTHVTPTELVLCAVTHEDIDHFIIETSKPNVLVRWNLVAHRLGY
                     EDIRLKEDTAYDSTVLDQKRF"
BASE COUNT          101 a           41 c           55 g           80 t
ORIGIN      
        1 atgacacatg tgacacctac agaactagtt ttgtgtgctg ttactcatga agatattgac
       61 catttcatca ttgaaactag taagccaaac gtattagtta gatggaattt agtggcacac
      121 cgtctagggt atgaagatat tagattaaaa gaggatacag catatgatag cacagtgctt
      181 gaccaaaaac gtttttaaaa cgaagacaag gaggtatata aatggctagc agtttatata
      241 atttggcttt agatttcagc aaagaattaa actacac
//
LOCUS       DAZKKW010000331          274 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.354, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000331 DAZKKW010000000
VERSION     DAZKKW010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..274
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.354"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          114 a           31 c           53 g           76 t
ORIGIN      
        1 agggttgacg cacttttgat gtcatgttat aataacgaag tcgtttggat gaggtagcta
       61 ataaagcttg attttatcga aaaaaacttc gaaaaagttg ttgacaaata acaataaaga
      121 tgatatgata atcaagtcgc tgctaatgag tgacaaagac gtttagaaaa aataaacaaa
      181 gaagttgttg acattctctt ctgaatctga taagattaaa gagttgttga aacaaaaaga
      241 ttagaccttt gaaaactgaa caaagtaaga caaa
//
LOCUS       DAZKKW010000332          271 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.356, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000332 DAZKKW010000000
VERSION     DAZKKW010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..271
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.356"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>271)
                     /locus_tag="ACG8PO_003093"
     rRNA            complement(<1..>271)
                     /locus_tag="ACG8PO_003093"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT           57 a           90 c           52 g           72 t
ORIGIN      
        1 catttgactc ctcttcagac ttactgcttg gacagacatt tccgatcgtc tgcattcctt
       61 agcctcctgc gtccctccat tgctcaaaca aaaacaacgg gtacaggaat atcaacctgt
      121 tatccatcgc ctacgcctgt cggcctcggc ttaggtcccg actaaccctg ggcggacgag
      181 ccttccccag gaaaccttag tcattcggtg gacaggattc tcacctgtct ttcgctactc
      241 ataccggcat tctcacttct aagcgctcca g
//
LOCUS       DAZKKW010000333          270 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.357, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000333 DAZKKW010000000
VERSION     DAZKKW010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..270
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.357"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT           93 a           36 c           57 g           84 t
ORIGIN      
        1 aatcgatcag agtgaagcag acatacaaat tgaaagtcta caagaactgg aacaaattgt
       61 tttagaaaaa ggttagtgaa ttaatggaaa taaggctata tatctgtgct agctattttg
      121 actaaccaaa aaaacttgct ataattcagg tgtagtaaaa ggtgctggta acacctttta
      181 cgaaaatgct tcggcagtga taatgggatt ggctttttaa gttttttatt ttgttcgcaa
      241 tccctaaggt catgtgaatg atcctaggga
//
LOCUS       DAZKKW010000334          264 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.358, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000334 DAZKKW010000000
VERSION     DAZKKW010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..264
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.358"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(34..>264)
                     /locus_tag="ACG8PO_003094"
     CDS             complement(34..>264)
                     /locus_tag="ACG8PO_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFQ0208320.1"
                     /translation="LKNLPETLDAQLRTKLQNLLTYEEGIYNAMIYPYSNGKIEAKIP
                     HIKTLKRLSYGFKSFENMKIRIFLINQLIQVK"
BASE COUNT           77 a           39 c           47 g          101 t
ORIGIN      
        1 agaaggactg accttctgtc tcagattttt ttgttatttc acttgaatca attgattgat
       61 caaaaaaatc ctgattttca tgttctcaaa tgatttaaat ccataagata atcgtttcag
      121 tgtcttgata tggggaatct tggcctctat tttgccatta gaatacgggt aaatcatagc
      181 attgtaaata ccttcttcat acgttaaaag gttttgaagt ttcgtgcgaa gttgtgcatc
      241 aagtgtttca ggaagatttt ttaa
//
LOCUS       DAZKKW010000335          264 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.359, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000335 DAZKKW010000000
VERSION     DAZKKW010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..264
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.359"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>264
                     /locus_tag="ACG8PO_003095"
     CDS             <1..>264
                     /locus_tag="ACG8PO_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0208321.1"
                     /translation="SEITGKANNYTDGKVSEINSQLTASINEVDTTAKDAQTKANANA
                     TAIDELDNKIDERINDTATTTLTVTNGNTGSAKLYREGKTVSIY"
BASE COUNT          103 a           46 c           51 g           64 t
ORIGIN      
        1 tctgaaatta ctggtaaagc aaacaattat accgatggga aagtatcgga gattaattcg
       61 cagttgactg cttcaattaa tgaagtagac accacagcta aggatgctca aacaaaagcg
      121 aatgctaatg cgactgctat agatgaatta gacaataaga tcgatgaacg cattaatgat
      181 acagctacta ccacattaac agttacaaac gggaataccg gatcagcaaa gctttatcgt
      241 gaaggaaaaa cagtttctat atat
//
LOCUS       DAZKKW010000336          247 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.denovo.360, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000336 DAZKKW010000000
VERSION     DAZKKW010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..247
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.denovo.360"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>247
                     /locus_tag="ACG8PO_003096"
     CDS             <1..>247
                     /locus_tag="ACG8PO_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFQ0208322.1"
                     /translation="SKAIIALKPEGRKAVDIENSINEWLQSVPKNLFKSITFDCGKEF
                     SNWKSISNTNDIDIYFADPGTPSQRGLNEHSNGLLRK"
BASE COUNT           88 a           47 c           43 g           69 t
ORIGIN      
        1 tttcaaaagc cattatcgcc ttgaaaccag aaggccgtaa ggcagttgat attgaaaatt
       61 cgattaatga atggcttcaa tccgtaccca aaaatctttt caaatcaatt acctttgatt
      121 gtggaaaaga attctctaat tggaaaagta ttagtaatac gaatgatatt gatatttatt
      181 tcgcagaccc aggaacgcct tcccaacggg gattaaatga acattcaaac ggactcctcc
      241 gaaaaga
//
LOCUS       DAZKKW010000337        17999 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.guided.054, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000337 DAZKKW010000000
VERSION     DAZKKW010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17999
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.guided.054"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..313
                     /locus_tag="ACG8PO_003097"
     CDS             <1..313
                     /locus_tag="ACG8PO_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HFQ0208323.1"
                     /translation="LELLREGQQGVTLRTFESMYFQKKLVTDNQAIAHYQFYHSENIF
                     LLQERDLDELPEFLHTPFQPIPSELLEFFDAENWAKRFFTTDSAIYEQYEYDLSSVKN
                     K"
     gene            complement(456..1397)
                     /locus_tag="ACG8PO_003098"
     CDS             complement(456..1397)
                     /locus_tag="ACG8PO_003098"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFQ0208324.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVVLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNKHVDLIVALTKANQLDDMFRSIQSIITENTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEAKLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAEGM
                     VRTIVSEFASIAAKEGITLDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAVWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            1523..2644
                     /locus_tag="ACG8PO_003099"
     CDS             1523..2644
                     /locus_tag="ACG8PO_003099"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFQ0208325.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALGGTWRTIDGMDLLFRQRKISYLLTNWQGKNTAVIESAQVLGLDLLDKP
                     TDQTIRLASSYALGELIKDTLLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTRKLHLDRRNVLVSKVKVIIAADVTNPYTGQQGAAWQFGRQKGGTDTTLAFLD
                     KQAEKIAQQIKDVWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFELVSQITGLEKQI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDRELEGLFDFLPIV
                     LSIQLGPIDLEEAMEHRQTLEKTEILGQTLSRFFNILPK"
     gene            2902..4404
                     /gene="eat(A)"
                     /locus_tag="ACG8PO_003100"
     CDS             2902..4404
                     /gene="eat(A)"
                     /locus_tag="ACG8PO_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection-like protein
                     Eat(A)"
                     /protein_id="HFQ0208326.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQAFVYFPQQTKDKERLTYYVLNEIMDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEIAKNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKKVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDALTMNSQVSHHKRLLSVENLQLGYENLLFEPVHF
                     TIEPHQRMAISGPNGAGKSSIIHYLLGTFNGKVVGEKSQPKHLTISYVRQNYEDNRGT
                     LAEFAKKNRVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYI
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLEKI"
     gene            4471..4998
                     /locus_tag="ACG8PO_003101"
     CDS             4471..4998
                     /locus_tag="ACG8PO_003101"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFQ0208327.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQALAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVVDRLNLPMTADERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECMEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELISYE"
     gene            5210..6271
                     /locus_tag="ACG8PO_003102"
     CDS             5210..6271
                     /locus_tag="ACG8PO_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MupG family TIM beta-alpha barrel fold protein"
                     /protein_id="HFQ0208328.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQNRLIELGSEAKKNKLELMVDISGEALKRAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPNDIDELKEAAADFSQIEAWHNYYPRLETGLDKEWYQEK
                     NQWLKTQGFLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSEHVQEQFASFQKEQTVLLHTEPVDEVFYEYILGKHTNRQDDARDVIRSADARF
                     REIPPIPVRNTAKRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            6274..7170
                     /gene="murQ"
                     /locus_tag="ACG8PO_003103"
     CDS             6274..7170
                     /gene="murQ"
                     /locus_tag="ACG8PO_003103"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFQ0208329.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELPSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISEHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEAAQAKLAEANGFVKGTL"
     gene            7184..9166
                     /locus_tag="ACG8PO_003104"
     CDS             7184..9166
                     /locus_tag="ACG8PO_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose PTS transporter subunit IIA"
                     /protein_id="HFQ0208330.1"
                     /translation="MAKNELTLEELAKQIYQGAGGMGNVESIVHCMTRVRMKVINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQEMVDLAGVKLGEKLPSASATTAS
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAGTWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALATGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKAVPQASA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVISSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAADK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(9236..9682)
                     /gene="nrdI"
                     /locus_tag="ACG8PO_003105"
     CDS             complement(9236..9682)
                     /gene="nrdI"
                     /locus_tag="ACG8PO_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFQ0208331.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEYEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQRFGFPFLADYELRGTPSDVTRVYQILAEKNK"
     gene            9893..11317
                     /locus_tag="ACG8PO_003106"
     CDS             9893..11317
                     /locus_tag="ACG8PO_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid/polyamine antiporter"
                     /protein_id="HFQ0208332.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNVASIVMSTIFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFINNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGFLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            complement(11494..12267)
                     /locus_tag="ACG8PO_003107"
     CDS             complement(11494..12267)
                     /locus_tag="ACG8PO_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0208333.1"
                     /translation="MTLLIKQELFKLIKKKSTAVLSVLLVALLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMESQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSIYLYLLAIIVTVLMKLILFPSISFTEKISTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIALFLALGYLVFKRKNV"
     gene            complement(12264..13169)
                     /locus_tag="ACG8PO_003108"
     CDS             complement(12264..13169)
                     /locus_tag="ACG8PO_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0208334.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSEGTIRIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVKQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDQAARTILTDAGYPLEDPDKIKLLKKNEELLGTVIQLLSAENVHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            complement(13330..13650)
                     /locus_tag="ACG8PO_003109"
     CDS             complement(13330..13650)
                     /locus_tag="ACG8PO_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HFQ0208335.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGLLLISIIGIKI
                     TSGH"
     gene            14123..14809
                     /locus_tag="ACG8PO_003110"
     CDS             14123..14809
                     /locus_tag="ACG8PO_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HFQ0208336.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            14811..15755
                     /locus_tag="ACG8PO_003111"
     CDS             14811..15755
                     /locus_tag="ACG8PO_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HFQ0208337.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            15954..16598
                     /gene="pcp"
                     /locus_tag="ACG8PO_003112"
     CDS             15954..16598
                     /gene="pcp"
                     /locus_tag="ACG8PO_003112"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016920 - pyroglutamyl-peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HFQ0208338.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            16677..17954
                     /locus_tag="ACG8PO_003113"
     CDS             16677..17954
                     /locus_tag="ACG8PO_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0208339.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
BASE COUNT         5732 a         3204 c         3858 g         5205 t
ORIGIN      
        1 tctagaactg ttgcgggaag ggcagcaagg agtgactttg cgaacgtttg aatctatgta
       61 ttttcaaaag aaactagtga cagataatca agccatagct cattatcagt tttatcattc
      121 tgaaaatatt tttttgcttc aagaacgtga tttagatgaa ttaccggaat ttttgcatac
      181 gccatttcaa ccaatcccat cagaactgtt ggaatttttt gatgcagaaa attgggcaaa
      241 acgttttttc actactgact ccgctattta tgaacaatat gaatacgatc tttcctctgt
      301 taaaaataaa taaaagtgcc aaccaatcat agatgaaaat aaacaagcgc aattcttatg
      361 gcaacatgag aattgtgttt ttctttttga actttcatgg gggtaaaaaa atgagaacca
      421 gctgctgatc cagctgattc tcatcagaga gccttttatt ctgcgccaag gattccttct
      481 tttgcatgga ctaattgggt caaaaaggca caatatggtg tagcaatatc atatttttgt
      541 ccttttcgcc aaacggcacc attgatgtag tcgatttcag tcaaacgatg gtttttgatc
      601 aggtcttggt acatagatgg gtaatgcaac ccgatgttgt caggatcata agtagaagca
      661 atatgttggt acacttcttc ttgatctaat gtgattcctt cttttgctgc gatagaagca
      721 aactcagata caatcgttcg aaccatgccc tctgccgctt tttgcgcacc taattcagca
      781 atattacaat ccaatatcgt gcataatcca tttaatgttc cgtttacaca agcttttcgc
      841 caaatggaat aacgaacatt atgactatag attggattca attttgcttc ttgaaagact
      901 tctacaactt tttttgtaaa tgcttcgcct tctggttcca agttctctag ttcgatttct
      961 ccatcaccta ataatgtgac ttttccggga ccagctagac cagctgtcca catcgtgatc
     1021 ccaaataaga tatttttctt tggtacatat ttttccagta catcttcatg acctaatcca
     1081 ttcagtaaac acaaaacata agtattctca gtaataatgg attggatcga acgaaacata
     1141 tcatctaatt gattcgcttt tgtcaaggct acgatcaaat ccacgtgttt gttgctttcg
     1201 atgatttctt ctggtgaata gattggcagt tttgcaacta cttctttgcc attaaagtca
     1261 gcaatcaatc catttgtacg aatggcttca atgtgagcag gccagcgatc gatcaagacg
     1321 acctcatttc cgctttgatg caacattaat ccgattcggc tgcccattgc tccggctcca
     1381 gctattgcga ttttcatgtt ttttctctcc tttattcaat gagttccctt ttagtatata
     1441 gaaaaacaga agaaaatagc attgttttgt ttgaaaaaag tgaaaaaaag tgtcttactt
     1501 aagaagaaag tgagggatat agatggatat tttggcggca atcgattcat ttaaaggaag
     1561 tgcttcttca tttgaattaa atcaggcagt tctaaatggt tgggaagacc gtaaaggaag
     1621 aaaaatcaat gtgccgattg cagatggcgg agaaaatact gcagaagcaa tctattacgc
     1681 gctcggggga acctggcgta cgattgatgg gatggatctc ttatttcgtc agcgaaaaat
     1741 cagttattta ttgacgaact ggcaaggaaa aaataccgca gtcattgaat ctgcacaagt
     1801 attaggatta gatttacttg ataaaccaac ggatcaaacc atccgacttg cttctagtta
     1861 tgccttggga gagcttatca aagatacact gcttcaagaa gtggaacaaa tcattgtcac
     1921 tttaggagga agcggatgta gcgatggtgg tctaggatta ctccaatctt tgggtgcttc
     1981 tttagaaggg tacacagaag gaaatccttt gttatctact agaaaacttc atttggatcg
     2041 aaggaacgtt cttgtttcta aagtaaaagt gatcatcgct gcagacgtga ccaacccata
     2101 tacaggtcaa caaggagctg cttggcaatt tggaagacaa aaaggaggaa cggatacgac
     2161 gttagctttc ttggataaac aagctgaaaa gattgctcag cagataaaag atgtttggga
     2221 ggtggattta gatgacatcc caggaagcgg agcagcaggc ggtattggcg gagccctttt
     2281 tttgttagga gcgaagatgg tctctggttt tgagttggtc agccaaatca ctggattaga
     2341 aaaacagatc gaacaagcgg atgtcatcat tacaggagaa gggcggattg atcagcaaac
     2401 aatccacgga aaagttccat atggcgtagc agtgcttgca aaaaaatatc gaaaaccaat
     2461 aatcgctatt tgcggaagta gagaccgaga gctggaagga ctctttgatt ttctccccat
     2521 tgtcttaagc attcaattag gaccaatcga tttagaggaa gcaatggaac acaggcaaac
     2581 attagaaaaa acagaaatct taggacagac actcagcaga tttttcaaca ttctcccgaa
     2641 gtagttgaca aaaacaattt ctttatgcta tcgtaatttc aaatatagga caaagtgagg
     2701 gatccgtgca ttgaaaaatt aagtcgattg atgaaagctg cattcagact gtaccttgtg
     2761 gagacaaggg agcagtgtgc agattttcgg gattggcttg gttctttcga tgtatgggtc
     2821 tttttgtgtg ccccaaccct tgcatcctct ttagccagtt ctgaaaatag gactggtctt
     2881 tttttacgag gtgatggaat aatgtctaaa atcgaaataa aaaatctgac attcggctat
     2941 gacagccaag gcacattatt atttgaacaa gccaatctaa actttgacac acaatggaaa
     3001 ctaggactta tcggacgaaa cggtcgagga aagacaactt tactgaatat cctgcaaaac
     3061 aaactccctt atcaagggca agtgatccat cagcaagcgt ttgtctattt cccgcagcaa
     3121 acaaaagata aagagcgttt gacctattat gtgttaaatg agattatgga ttttgagata
     3181 tgggaaattg aaagagagct ccaactgatg caaacagatc ctgaaatctt atggagagaa
     3241 ttcagcacac tatcaggagg agagaagaca aaagtcctac tggcactctt atttgtggat
     3301 gacactcatt tcccgttaat cgatgaacca acgaatcatt tggatatctc tggcagaaaa
     3361 caagtagcgg cttacttgaa aaagaaaaaa caaggattca tcgtggtgag tcatgatcgg
     3421 ggatttatcg atgaagtagt ggaccatgtt ttagcaatcg aaaagagcca actggaactt
     3481 tatcaaggga atttctctat ctatgaagaa cagaaaaaac ttcgtgatga atttgaaata
     3541 gctaaaaatg aaaaattgaa aaaagaagtc agcaggctaa agaaaacagc agctgaaaaa
     3601 gcagaatggt ctcgttcccg agaaggggac aaaacaaaga aaaaagtcgg attcatcgat
     3661 acggaatcta ggcgagtgaa taaaggagca gtaggcgctg atgctgcacg gacgatgaaa
     3721 cgatctaaag caatcgtgaa tcggatggaa acccagatca gtgagaaaga aaaattatta
     3781 aaagatatcg agtatatcga tgctttgacg atgaatagcc aagtgtctca tcataaacgg
     3841 ctgttaagcg tagaaaatct tcaattaggg tatgaaaatc tgttattcga gccagttcat
     3901 tttacgatcg aacctcatca gcggatggca atttcaggtc ctaacggcgc gggaaagtca
     3961 tccattatcc attatcttct ggggacattc aacggcaagg ttgtaggaga aaaatcccag
     4021 cccaaacatt tgaccattag ttacgtacgc caaaattatg aagacaatcg agggacattg
     4081 gcggaattcg cgaagaaaaa ccgagtagac tatcaagcat ttttgaataa tctccgaaag
     4141 cttgggatgg aaagagatgt ttttcataat aaaatcgaac agatgagtat gggacaacgg
     4201 aaaaaagtgg aattggctaa atctttatcc caaccagctg agctctatat atgggatgaa
     4261 ccattgaatt atttggatgt cttcaatcaa gaacaattag aacaactgat tttgaacgtg
     4321 aaacctgcta tgttactagt ggaacatgat caaacctttc tagataaagt atctactgag
     4381 attatttctc ttgagaaaat ctaaaccttg taatctcaaa gacaacaagg tatggttaag
     4441 gagaactttg tcaacgaagg agagaatcac atgctttatg taacgagaca cggagaaaca
     4501 acttggaacg cacaaggatt agtttgcggg cgagcagatg tgccgctgac tgaaaaagga
     4561 cagatgcagg cacaggcact agcagaaaag gtagtagatt tgcctgttcc aatcacgaaa
     4621 atcatccatt cccctcttca acgagcaaga gatacagcac aagcagtagt tgatcgtctc
     4681 aatttgccga tgaccgctga tgagcgattg gtagaaatgg attttgggga ttatgacgga
     4741 ctgccaagca aagatgaaaa ctttcaaaaa gcgcgtttag cgtttgctgt acgtttccca
     4801 aatggagagt cggtcttaga tgtgtacgca cggatcgttc ctttattgaa agaatgcatg
     4861 gaagacgaag aaaatgtcta cttgttagtc tgtcataatg ccttgataag agtgatcaat
     4921 gcttattttc atccaatgcc taatgaaggg ttctttacat tcatggtaga caatacagaa
     4981 ttaatttctt atgagtaatt agacttttgt gttatttaaa taaaatgttt caaggcactg
     5041 cttttgctta gcagtgcctt ttttgtggct gtgtcaattc ttttggcatt ataaatccaa
     5101 tttttagaaa taaaatttaa ttattttaac aaaagtattg aaattttgtt tctttagata
     5161 tatactctag tcatagaaat gaagacggat acagaaaaag agggataata tgtttggctt
     5221 ttcaatattt atgaatgatt ctttgtcaga agagaagaaa cagtatatcc gccaaatggc
     5281 atccaatggc tttgtcggta ttttcacttc catgcatata ccggaagatg atgtttcggc
     5341 ttatcaaaat cgattgatcg aactaggaag cgaagcgaaa aagaataaac tagagttgat
     5401 ggtcgatatt tcaggagagg cattgaaacg agctggcttt tcattagaag atctaacacc
     5461 cttgaaagca attggtgtaa cagggttaag aatggattat catatttcta atcagcagat
     5521 cgccgactgg tcacatcaat tgaaaatcag tttgaatgcc agcacgatca cgcctaatga
     5581 tatcgatgag ttgaaagaag cagcggctga tttctcacag attgaagctt ggcataatta
     5641 ttatccacgt cttgaaacag ggttggacaa agaatggtat caggaaaaaa atcaatggct
     5701 caaaactcaa ggattccttg tacaaggctt tgtaccagga gatacagagt tgagaggtcc
     5761 actgtatcaa ggattaccaa ctttggaaga acatcgaggg atccatcctc tggcaggtgc
     5821 tcttgatcta ttagcaagta acacggatat tgtttatatc ggagatgcag gtctgtcaga
     5881 acacgttcag gagcaatttg cttctttcca aaaagaacag acagtcttgc tccatacgga
     5941 acctgtagat gaagtttttt acgaatatat attaggaaaa catacgaatc ggcaagatga
     6001 tgcaagagat gtcatacgaa gcgcagatgc gcgttttcga gagattccgc caatcccagt
     6061 aagaaataca gctaagcgta tgaaaggatc tattacttta gataacgaaa agtatttgcg
     6121 ctatatgggt gagatccaat taactaaata tgatttgcca gcggatgaaa aagtgaatgt
     6181 agtagccaaa gtcatcaaag aagaactacc gctgatcaac caaatcaaag ctggtatgaa
     6241 ctatcaattt ataagaaaag aaggaagata aaaatgagca aaatcaattt agagaaccta
     6301 acaacggaac gcagaaacca agaaactttt ggattagatg aaatgagtat ttctcaagca
     6361 gtccaactca tgaacaaaga agatcagaaa gtggccgctg ctgttgaaaa agaactgcca
     6421 agtatcgagc cagtgatcgc caacacgatt gaatccttta aaaaaggtgg tcgcttgatt
     6481 tatatgggag ctggaacgag tggtagactt ggtgtattag atgctgctga atgtgtaccg
     6541 acattcggtg tagaaccgga aatggtcata ggcctaattg ctggtggaga agaagcaatg
     6601 actgtagccg tcgaaggagc cgaagattct tatgaattag ggcaagaaga tctaatcaag
     6661 ctgaatttaa caaaaaatga tatagttatt ggtatcgcag ctagcggtcg tacaccatat
     6721 gtgattggtg gattagatta cgcaaaatcg attggtgctg ttacaggaac gatttcttgc
     6781 aataaacaag cagaaatcag cgagcatgcg gatttcccaa tcgaagtcga tgcaggacca
     6841 gaatttttga caggctctac tcgattgaag tcaggaacag cacaaaaact gattttgaat
     6901 atgatttcta ctatcagtat gatcggtatc ggtaaagtat tcaataactt gatggtcgac
     6961 gtcaaaccaa caaatgaaaa actaattgaa cgctcaaaac gaatcatcat gcaagcgaca
     7021 gaagctgact acgaaacagc tgaaaagtac tttgaggaag cagatcaaaa tgtcaaactg
     7081 gctatcgtca tgattttgac aaatagtgat aaagaggcag ctcaagcaaa attagctgaa
     7141 gcaaacggtt ttgtaaaagg aaccttatga gggggaaaag accatggcaa aaaacgaatt
     7201 gactttggaa gaactggcaa aacaaattta tcaaggagca ggcggtatgg gcaatgttga
     7261 aagcattgtc cactgcatga ctcgtgtacg tatgaaggtc atcaatgatg atcttgtaaa
     7321 tgtctcggaa ttaaaagcga ttccaggagt acttggcgta gtagaggatg aacagctcca
     7381 agtcatcatc ggacctggga aagtgaacaa agtagcgcag gaaatggttg acttagcagg
     7441 cgtgaaatta ggagaaaaac ttccttctgc tagtgcaact acagcaagtt cttctgcatc
     7501 tggaaaagac cgtgtaaacg aacgggcaca agaaatgaaa gcggcacaaa aatcgaaaca
     7561 aaaaccctct aaaatcaaat cggtattaaa agacatctct aatatctttg taccaatgat
     7621 ccctgcattt gtcggcactg ggatcgtggc agggattgcc gcagttttag caaacttagt
     7681 cacagctgga acactagacg caggaacatg gcaacaatat atcgatgtga tgaacatctt
     7741 gaaaaacggg atgtttagct atctagttat ctatacaggt atcaatgctg cacaagtttt
     7801 tggtgcaacg ccaacgcttg gtggagttat cggggctgtt gtgatgctga cagggatgaa
     7861 tccagaagca ccactgaaaa acttgtttac cggtgaagct cttgcaactg gacaaggcgg
     7921 gatcttaggt gttatcttcg cagtttggtt attgtcaatc gttgagaaaa aactgcataa
     7981 agtagtacct gatgcggtag atatcatcgt tacgccaact ctttccctga ttgccatcgg
     8041 tctgatcgaa attttcttca tcatgccttt agcagggttc atttctgacg gcatggtcgg
     8101 tgggatcaac tgggtacttg gtgttggagg agcatttgcc ggcttcgttc taggtacatt
     8161 attcttgcca atggtcatgt tcggactaca tcagatcttg acgccaatcc atgtacaaat
     8221 gattgatgaa actggtcgta ccttattgct tccaatcctt gcgatggccg gagcaggaca
     8281 agtgggtgca gcattggcat tatggatccg ctgcaaaaaa gacaaagaat taacagaaat
     8341 gatcaaaggg gctttaccag tcggaatctt aggaatcggc gaaccattga tttacggagt
     8401 aacattacct ctaggacgtc catttattac tgcttgtatc ggtggaggta tcggtggggc
     8461 agtgatcgga atgatcggaa atgtcggagc aatcgctatt ggaccttcag gagccgcttt
     8521 gattccattg atctctgatg gtaaatggtt aggctatatc cttggtttat tggctgctta
     8581 cgctggtggc ttcgttgcta cattcttctt cggtattcct aaagaccgtt tagaaaaaga
     8641 agaactagcg accgaaacag ccacagtaaa tgaagtacct gaaaaagcag tacctcaagc
     8701 ttcagctgaa agtgtgacta tcttatcatc agttgctgac ggaacagttg tttctttaga
     8761 taaagcaagt gacccagttt ttgcacaaaa aatgatgggt gaaggttact ttgtagaacc
     8821 agaaaacgga catatctatt ccccggttac tggagtaata agttctgttt tcccaacaaa
     8881 acatgctatc ggcattacga caccaaatgg cttagaaatc ttgcttcaca tgggcatcaa
     8941 cacagttgac ttaggcggta cgccattcga tgtgaaagta acagaaggac agcaagtgac
     9001 acctgatacg cttgtagcag aagctgactt agaagcaatc aaagcagcag acaaagaaac
     9061 ctccatgatc gtcttggtaa ccaatatgga tcgtgtaaaa aactttgtct tggaaaaaac
     9121 aggtaaagcg aaagcaaaag cacctgtgat ggatgtcaaa gcttaataag aatagaaaca
     9181 atagagaaca taaaaagaag aagccgaaat ttatttttca gcttcttctt ttgtgttatt
     9241 tatttttttc tgccaaaatt tgataaacgc gtgtcacatc agatggtgtt ccccgtaatt
     9301 cataatctgc caagaaagga aagccgaatc tttgtgcgta ttgtttggct gtcagacaat
     9361 attgattgtt gaagttttta ttaccgctac cgactacacc aaggcaaagt ttatgattgt
     9421 cttcgtattc cagatattcg cgcatcgttt cggtcaagat ttctgtgtca ccgttgtcta
     9481 acccattccc gccgtctaaa taagtaggaa cgaaagtaaa aaatggttct gtttctttcg
     9541 taaaatcact attttcatgg atttctttta aagtaacttc aggattgaca ggatcttctg
     9601 catgcatttt ttcagcatat tctgctagat gtttggcaaa agcacgcgta tttcctgaaa
     9661 tcgatatata taaaatgttc atggatcatc ccccaattct ttccctttag tatagcatct
     9721 ttttaagttt ttttggtggt gtaatttgga aagggactag gcgctttgct tgtataatga
     9781 gaaaaaagtc ggtagaatta tatttctatt aaagttttta ttaattaagt aaaaataaaa
     9841 aaggtttgtt atactttttg ataaaagcgg ttttactttg gaggatgctt acatggatac
     9901 agagcaacag aaaaaaggaa ttggtttgat tccacttgct gccttagtga ttggttcagc
     9961 gattggaggt ggcgtatttg gtattatgac ggacatctcc ggatcggctg gtggcccagc
    10021 tatcttcagc tgggtactcg taggaacagc aatgttgatg ctctccttat cgatcaataa
    10081 tatcaataaa aaacgtccag atttaaaagg cgggatcttt agttacgcag aagaaggttt
    10141 tggccgtttc gctggtttta tcagcggttg gggttactgg ctcacatgct ggttaggaaa
    10201 cgtggcgttc gcaacattgt tgatgagtgc attaggttat ttcttccccg tattcgaagg
    10261 tggtcaaaat gtggcatcga tcgtgatgag tacgatcttc ttatggggat atgcatggct
    10321 agtgaatcgt ggagttgaaa atgctagctt tgtcaatgct atcgtcacat tttgtaaatt
    10381 agtgccatta tttttattta ttgtagtagc tatcagcacc ttttcgatcc aacaattcat
    10441 taataacttt tctagcgata tcatattgaa tgcgaatggg cagccgatcc cgttttctag
    10501 tcaagtattg agttgcatca tggtgatgct ctgggtattc gttggtattg aaggagcttc
    10561 tgtcgtaggt tcacgagcaa aaaaacaatc ggatgttggg aaagcaacga ttctaggaat
    10621 attcggtttg atcattattt acgtcttggt ttccatgctt ccatatggca caatgacgat
    10681 gaatcagctg caaacgatca aagcgcaacc tgctatggga tatgtatttg aaatgatggt
    10741 tggtaaatgg ggggcggtcg tgatcaacgg cggtttgatc atttctttag ttggggtttg
    10801 gctctcatgg acaatcttgc cgattgaaac aatggggaat atggctgagg acggactttt
    10861 gcctaaagca tggggaaaac tcaacaaaaa tggggcacca acttatgcta tcgtattgac
    10921 gacgttatgt acgaatgtct ttttactttc attattattc actgacagtg catataactt
    10981 tgcttataca cttggtacag cagcgatttt cttcacgtgg cttttcttag ggctgtacca
    11041 aatgaagctt tcttatcagc ggaaagagtg ggtccaattt ttagtagggt ttttagcaag
    11101 tgcttttcaa gtatgggcca tgctagttgt agcctttaat gaagtgatgc ttgttcttgt
    11161 tcttttctta ccaggtattt atttttacct caaagcacgt aaagaacaaa acgaacctgt
    11221 acaacatatc cacttagata agattttact tatcggtgtg acgatggtcg gagtaatcgc
    11281 aatggtttta tttgccactg ggatgcttca cgtatagaag tataaagaga ggctgtgaag
    11341 cctgtggttt attcaaaaaa gaagcggtga caacattcgt gtcatcgctt ctttttttga
    11401 ataaatggtg ttcaagaagt agcttcttct aaaaataagc cgaaatttct tgaaactggc
    11461 cacttctgtc acaacttctt tgttaatgaa ccatcataca ttttttcgct tgaatacgag
    11521 gtatcccaat gctaagaaca aagcaatgta agctaaattg ccaaacagca tttgattagt
    11581 agaaagatga gtcaattgct tcataatctc ttcgttgcca atttggtttt gcagattcag
    11641 catactgatt ggattccatt tgatccactc ccatttttga atgagtgcga tgaggatacc
    11701 agatatgatt gatgaagcaa aataaaagac gatccctgct gagatggagg caccggaact
    11761 attgataaaa caagcaatca tcaacactaa acttaaaatt agccataaac cgatataact
    11821 acccaaagta taagtgaata agctttggat cagtgtctga ccagtagaaa tcttctccgt
    11881 aaaggaaatc gaagggaaaa ggatcagttt catcagcacg gtgacaataa tagctaaaag
    11941 gtataaataa atagaataaa taactaatgt gatccattta cttactaata tttctcctcg
    12001 agaatacttt cgataaagga gatttttcaa tgtaccatat tgtgattcca ttgaaatgat
    12061 cgtacttgca gcagcaatca tgataaagac aatccaagat gtggcactga aaagctgagc
    12121 agtcatttca actggatcaa tgatcgtcgt gtattttttg gctagaagtg ctgttcctat
    12181 gagcaaagca actaatagaa cagataaaac agcagttgac tttttcttga tcaatttaaa
    12241 taattcttgt tttataagca acgtcatttt tcttcttccc cctttactga atccagaatt
    12301 tccaataggg aagattccaa atcattcgtc agatgctgga catctgtcac atggacattt
    12361 tctgcagaca ataactggat aacagttcct aataactctt cgtttttttt tagaagcttg
    12421 attttatcag gatcttccaa gggatatcca gcgtctgtca agatcgttcg agcagcttga
    12481 tcatttgtcg tagagataac gatgaaatct tgcgctgttt ggttcaattc agccattgta
    12541 cattgtttga tgatttttcc ttgattgatc aggatcagct catcgaccat tttttcaagc
    12601 tcgctgagta agtgactgga gatcaagaaa gaggtgccct gagtagccaa gtcggtaatg
    12661 atttttcgca catctcttgt tgcttgtgga tccaaaccgt tcatcggctc atctaaaata
    12721 acgatagacg gatggttcag taaggcaaga gcaattccca atttttgttt cattcccagc
    12781 gagtaggatt tcgctttttt gtgaatgtat ttttccatct tgagctgttt cacgatagtg
    12841 tcgatagcag atacatcgtt tgtttcagag aataacttca gatgatcata acctgttaag
    12901 aatggataga tacccggata ttcgatcaat gctcctactt gttctaatcc tttatgacta
    12961 gagggagaaa tctcattttc accaatacgg atcgttcctt ctgagtaatg gatcagtcct
    13021 aaaatagctt tcatgatcgt tgtttttcca gcgccattag gacctactaa tccaacgatt
    13081 ttgccaggag ataagctaaa actgacatcg ctgagggctt gtttgtgacc aaagttcttg
    13141 gataattctt ggaccgaaag acttttcaaa aatattcact ccttttctat agatagtaca
    13201 agcatacacc tgatgagtag agaagtcgct aggtcattag tcttattttc ttgttttttc
    13261 atagaaagag gaatatcaaa taaaaagccc cgctttttct gatcagtcct tgtctgcttg
    13321 tctaagtatc tagtgaccgc tagtgatttt gatgccaatg atgctgatga gcagcaaccc
    13381 gacaaagaac caagtggaaa gattcagctt atcatgaaac aagaccacac caataatgac
    13441 cgaaccaact gcgccaatcc ctgtccaaat aggataagag agactcatag gcaaactttt
    13501 gattgccttt gacaaaaagt aaaaactagc aatcattcca atcactgtgt aaaaagaata
    13561 attgatttta gaaaaaccat cactccattt catcatcgtt gaccagaata cttccaaaat
    13621 tcctgctatg attaattcca tccatgccat acatacgttc ctccttcaat ataaaaaata
    13681 cacaaaaaga ggagaatcat cccttcaaag acctaatgga atgatcatcc tcttttttac
    13741 tacccggtgg cagatcgtat atctttttct gtagtatagc acacctatcc agatatcggc
    13801 aatctcatac tttttagtaa agtgtaacaa gcattgacgg atatatcgtt agcagaaaag
    13861 aagatgaatg ctttgcaatt tcatagaaag gagttgttat ttattaatca tttaaaaaaa
    13921 cagtaactag accttatttt tatatattag caggcaataa attaaaaaaa gctttatctt
    13981 acaataagta aggtgaatta tataataaaa aaataacgaa aaatatttaa aaatagtgtc
    14041 tatttcgtga gaaaacaact atatttttta tgtgttttgc tttataatta aaaaaatatt
    14101 gtcattttat ggaggaaaat atatggaaaa ttatcttagc cttttaggcg tcttgattgt
    14161 tattctcggg tttgcattta aattagactc gatattgatc gtaatggttg ctttagttgt
    14221 tacagctttg actggcggtc ttggcattca aggaatgtta gaacttttag gtacaagttt
    14281 tgtaaacaat cgcgggatgg cgatttttat catcattatg ttggcaacag gaacgcttga
    14341 aagaaatggt ctgaaagaat cagcggcttc cttgatcaag cgattcaaga aagtatctgc
    14401 tggtatgatt gtagatattt atgggatctt tcgaatgatc tttgcggctt ttaacgtgag
    14461 ttttggtgga gttgctggat ttgttcgtcc aatcatttta ccgatggcat tggggaccat
    14521 cgaatcaaaa gggttagaag tccatcctaa gcatgaagaa gaactaaaag gtatggcttc
    14581 agctatggaa aatgtctgct ggttctttgg ccaagtacta ttcatcggag gagcaggggc
    14641 gttgctagtc caatcaacac tgaaagatct aggatatgac gtaacattgg gacagcttgc
    14701 tttagtggaa gttccagtgg ctatcacggc tttagttgtg gcaagtgtct acttctatct
    14761 taaagaaaag aaattagcga agaaatatta tccaggaaag gggcaataag atggactttt
    14821 ttacaaatcc tgatatttta ttaggcgata aattactgga gatcctttat atcgtgatgg
    14881 gactcgtatt gatttacact ggagtaagaa atttaacaga taaaaccaat cctcatcgtt
    14941 atgggtcagc ctttttctgg acggtcttag gggtagttat tgcaggagga agatggcttc
    15001 cggcaatcgt gaacggtggc ttgatctttg cgatgaccat accagcaatt gctaagaaag
    15061 tcagcaaagg agaaagccgg cttccatcaa aagaatatat ggaaaaaatg tcagacaaac
    15121 taggaatgaa aatttttatt ccagctttga gtatcggtgt ctttgcgatc ttgtttgctc
    15181 tatttaccaa tttaggtgct ttagtcggcg tgggcgtcgg tgtttttgca gccatgctaa
    15241 tcttgatgtt cttttctcga gataatcgac caagtacatt tttagatgat gcagcagata
    15301 tgctgggcac agtagggccg ttaagcatgt tgccaatgct tttagcttct ctaggtgcgg
    15361 tatttacaag cgctggagtt gggacagtga tttccaatgc agtaggtacg atcgttccag
    15421 aaggaaatgt gaacatcggc atcatcattt acgctcttgg catggtgata tttacggtaa
    15481 tcatggggaa tgcatttgcg gccatcaccg tgatgacggt tgggatcggt gcaccattcg
    15541 tatttagtca tggagcgaat ccagcgttaa taggaatggt tgcattgact tgcggttttt
    15601 gtgggaccct gctgacaccg atggctgcta acttcaatat cgtgcctgta gcgatgctgg
    15661 agatgaaaga caaatacgga gtgatcaaaa accagttgtt tattgcgtta tttatgctag
    15721 ttttccaaat tgcttatatg attctattta aataatagaa gagaggggtt gtgacagaat
    15781 aattgtcgca acctcttcta acattgtagt ggaacactat ttttttataa gaatatatta
    15841 agtttgatct gttatgttat ttaaactatt gtttccttta tttacgctgt tcaaggaaaa
    15901 tggtatgata aaagataacc atatgttaaa tcaatcggac ggaggaacaa cagatgaaag
    15961 tactagtaac aggttttgat ccatttggtg gggacaaagt gaatccagcc tatgaagcag
    16021 taaaaaaaat gccagatgaa atcagcggtg ctgaaatcat caaagtggaa gtaccgaccg
    16081 tattcgaaaa aagcagtcaa gtagtcaaag aagccatcca acagcaccaa ccagatgtcg
    16141 tgatctgtgt cggacaagca ggtgggcggt cagcagtctc gttcgaacgt gtcgcaatca
    16201 atctagcaga agcaagaatc cctgataatg aaggaaatca gccattcgat acggctttag
    16261 aagaaaacgg tccagccgct tactttacct cattgccaat caaagcaatg acaaaaaatg
    16321 tacaagatca tggtctacca gcgtacatct cttatacagc aggaacattt gtctgcaatg
    16381 atatcatgta tcgtttgctt catttgatcg aaacacaatt cccggcaatt cgaggtgggt
    16441 tcatccacgt gccattctca cccgatcaag tgatcgatcg tccagtgggt acgccttcta
    16501 tgtctttaga agacattgct tcttctttga cttatgcagt tgaagcagca gtagaaaata
    16561 aagaagatat ttccggaaat gccggaacaa ctcactaatt tagcaagaaa cactggctgg
    16621 ttgctaacgt tatgttagta gccagctttt tgtaattaga aaggaaagaa acagccgtgg
    16681 aagaatggca atccgtcttt gaagaatggt ttccaaaaga aatcagcaaa agctatccca
    16741 tcaaaatttc taaacaatac acaagcagtc aacgttggga gatctatgca aaattaacga
    16801 aaaaacagcg ggaactagtc gataagcacc gccgatatct aatcagctct cgttttatgg
    16861 aagaacatta tttagctgca acagattggg tgttttctga cttcaagatc aatccgtttt
    16921 ttcgaacaaa acgaagccag caaaaattgt attgtgagtg tggtagagaa ttaaaagtgc
    16981 aatacatcgt taaatcgcca aaaaccggaa agatcctaaa actaggcatc aaccactttg
    17041 ccgatcacct gcatgtctct ccgacagtgg cagcttcgat ccatcaggga atgacaaaag
    17101 ttgatctggc cttagacgaa ttattatggc ttaagcagaa gaatatcgat tttcctgaag
    17161 gactatggca aaaatactgt tttgtcttgt accaaaatcg tcggatgaaa cagccatatt
    17221 taccggatat aaaattagct cagcggctcg cagaatttcg ccaagtagaa atgccgattt
    17281 acatagcgga ttatcaagca ttggaaaatg aaatcaagaa aataagtgaa cacatcaatg
    17341 ggcagtcaaa aaagcgacag ataaaaaaag aactatttga cgactttgca gaagaattag
    17401 taaaggatgt agaagaattc ttgatcaact atcgcgcatt tttacgtaaa gattggcaat
    17461 ccatcgtata tgaagaagtg ccagtacatc ctaatgctta ttttgaaaca tttatttccg
    17521 tgttgagaaa gacaaaacgt caacgaacac cagaagtcac tgctcagatg gaatattttg
    17581 cgaaaaacca acgattcatc caaccaaaaa tctatctatt tatttggaag caatattgtc
    17641 gctatggttt tacagaaggg ttcttcgaca gcattcctcg gatagtcaga aatggttttt
    17701 taaaagtatt gcgtaaagaa cgagaagcta tacaatctgc tgataagaaa gaccgtacag
    17761 tctcaaaaga aaaatggcaa ctagtagtga aagatatcca atctggaaat gtgcaagaga
    17821 caatcgacaa atggaaagga aaacattatc gttttactga agctcagaaa caagcgttgg
    17881 aatattatca gaagttagaa gaatcactgc gtttcaatga tgaggcaaga aagtatctga
    17941 aggaattact ataaaaaata gaggctggga cagaagcgtt taactccaag aaataagaa
//
LOCUS       DAZKKW010000338        13213 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.guided.073, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000338 DAZKKW010000000
VERSION     DAZKKW010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13213
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.guided.073"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(204..2162)
                     /gene="nagE"
                     /locus_tag="ACG8PO_003114"
     CDS             complement(204..2162)
                     /gene="nagE"
                     /locus_tag="ACG8PO_003114"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-specific PTS transporter
                     subunit IIBC"
                     /protein_id="HFQ0208340.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(2159..3031)
                     /locus_tag="ACG8PO_003115"
     CDS             complement(2159..3031)
                     /locus_tag="ACG8PO_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFQ0208341.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            complement(3373..4245)
                     /locus_tag="ACG8PO_003116"
     CDS             complement(3373..4245)
                     /locus_tag="ACG8PO_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFQ0208342.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            4373..4942
                     /locus_tag="ACG8PO_003117"
     CDS             4373..4942
                     /locus_tag="ACG8PO_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HFQ0208343.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            5009..5746
                     /locus_tag="ACG8PO_003118"
     CDS             5009..5746
                     /locus_tag="ACG8PO_003118"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFQ0208344.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            5977..7245
                     /locus_tag="ACG8PO_003119"
     CDS             5977..7245
                     /locus_tag="ACG8PO_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFQ0208345.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            7732..8196
                     /locus_tag="ACG8PO_003120"
     CDS             7732..8196
                     /locus_tag="ACG8PO_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFQ0208346.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            8180..8809
                     /locus_tag="ACG8PO_003121"
     CDS             8180..8809
                     /locus_tag="ACG8PO_003121"
                     /EC_number="1.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFQ0208347.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            9163..10641
                     /gene="msr(C)"
                     /locus_tag="ACG8PO_003122"
     CDS             9163..10641
                     /gene="msr(C)"
                     /locus_tag="ACG8PO_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HFQ0208348.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            10688..11134
                     /locus_tag="ACG8PO_003123"
     CDS             10688..11134
                     /locus_tag="ACG8PO_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HFQ0208349.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(11333..11848)
                     /locus_tag="ACG8PO_003124"
     CDS             complement(11333..11848)
                     /locus_tag="ACG8PO_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFQ0208350.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            11965..13167
                     /locus_tag="ACG8PO_003125"
     CDS             11965..13167
                     /locus_tag="ACG8PO_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0208351.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
BASE COUNT         4116 a         2424 c         2708 g         3965 t
ORIGIN      
        1 agaggttgta aaaaagctgt tctgcatcaa gtaataagaa gagtcaaata aaaaatagct
       61 cttcatatgt tacgtttggc tggacttatt accgcggagt tggcaggaga acgccgtttt
      121 tattcggaaa aaggagctga gacaagattt ttgtctcagc tcctgtttgt ttatcgataa
      181 aaaagtctgg ggaagacgat ggctcaggct ttgttggaaa cagagccgat tttatcattt
      241 gcttttacat cacgtgcggg ttcgatttct aagctttgga ctttgtcgcc atttgtgaat
      301 acaacgacca tatccgtgtt tttgcctgca gattgaatag cggcaagatc aactaaagca
      361 atcaattgac ctcttgcgac tttttgacct tcttttacat ataaagtgaa tggttcgcct
      421 tttaagtcaa cagtgttgat gcccatatga agcaagactt cgataccagc atctgtttgg
      481 attccgacag catgtttcgt tgggaaaatg tttgtgatcg taccagcgat tggagagaag
      541 atttctccat tttctggtaa tacagcatag ccgtcaccca tcattttttc cgcgaataca
      601 tcatctgaaa cttcagtgat tggaatcagt ttcccatttg cgatagcgta aatatcttgt
      661 tcgatcgttc caccggcaaa tgtttctgtt ttaaccacgg tgtttgtttg tgcaggtcgt
      721 gttgcagcac caccatgtag ttttgccatc tcatctgcaa cgaattgtac ttctgtacct
      781 acgatgactt gcgcattttt tccgtcgatg atcttcatac caggtacgcc agtggctttg
      841 attttatttt gatcgatcag gcctgtatct ttgacttgca aacgaaggcg tgtagtacag
      901 ttatcgatcg aagttacatt gtcggctccg cctaatgcag cgtagatttt gcttgcttgt
      961 gccgcatatt tattgtctga attagtcgtt gcagtatcgt catctgaaag atctgcttcc
     1021 tcgcggccag gagtcatcag atggaatttt ttgatagcaa agttaaagcc aaagtagtaa
     1081 atcactgctg ttactaatcc ttggacgatc aacatcaatg gctgattcgc aatcggattt
     1141 ttcaaagata ggaagtaatc gacaaatccg gcactaaagg caaatcctgc tgtccaatgg
     1201 aagaatgcac tcactgctaa agaaatccca gtgaaaattg catgcaagac atataatggc
     1261 caagcaacga acataaatga gaattcaagt ggttctgtta caccagtaaa gaatgaagca
     1321 aaagctgctg ctaacatcaa ggaagctgtt tttgctttgt tttctggacg tgcatttctg
     1381 taaatagcat atgctcctgc tggaagcccg aacatcatga ttgggaaaaa tccagcttga
     1441 tacattcctg ttacgccttt tgttccttca gaagccaaaa atttaccaat atcattgatt
     1501 cctgctacgt caaaccagaa cacggagttc attgcttgat gcaatcctgt agggatcagt
     1561 aaacggttga agaagccata aagacctgca ccaccccagc ctaaactcaa aatgaattta
     1621 gcaaaagaaa ctaaagcaga gtaaaccgga ggccatacga gcagtagcac tgcactgatc
     1681 agcaacatga caaaggcagc caagattggc actagacgct tgccgctaaa gaaagataaa
     1741 gccatcggta attttgcttc gtggaaacga ttataaagag ctgccgcaat caatccggca
     1801 ataataccaa taaatacgtt actgatctga gcaaaagatg gatcaactga ttctggtttg
     1861 atattcaata aagtagctac tgaatcagtt tttaaaacat ttactggaac ttcaaatgcg
     1921 accaaacctg ccaaggcagc agcacctgat ttgtccactg acatccctag tgctaaccca
     1981 acggcaaaca gcaaaggtaa ttgacctaaa actgcgttac cgccagcttg taaaaaatga
     2041 gcgacaatgt cgttactata agatgcccac cagtttccga tccctactag taaagcagca
     2101 gcaggtaaga ccgcaactgg aagttgaagg gaacgtccca ttcgttgtag atatgctttc
     2161 aatttttttc ctcctcatga ttgttaacat ttgtttgtac gattgcgctt cttaaacgct
     2221 cgatatggat ggccaagtaa cccagttctt ctttcgtgat cgcgagctgg tattctttgg
     2281 tcagcaacac acggattttt ttagaaatct tatagctttc tgggtatttt cgtttgacca
     2341 tttctacgat ttccgtgtct aaaacagcat attgttgcgc atgggttcgt tgtagaagca
     2401 gtcgtaaatg attcaccagt cttgaataat tcagtgaaaa tggttctgaa tggatatctg
     2461 tttccaattc tgattccact aaatggatga tgtcagatac aatcgtcact tcacggatac
     2521 tgcgatggtt attgttccga ccgcttcgtc cgctgtgaat atggattgcg atatatcctg
     2581 cttcgtcata agtatatgga atcccaagct cttttgtcag atactctact gcccattgag
     2641 caatactgaa ttcatcactg taaagcactt cgatttctct taaaagtttg ttgcggatga
     2701 cgataccgtt attaatgttc tccgcagaaa aagcaatgtg gtcggtcaaa gcgatcaata
     2761 catgttcatt caacttttcc atcaaaacag tttccgcatg atcaatgatc gtttctgctg
     2821 caaataaaaa gcgttcatca atctgattaa gtaaattttg caacttgatt tgaccttctg
     2881 gttccatgac aaatatccgt tcgacatcac gatcaaaaat caaatcattt ctttttctgt
     2941 caaatccaat cccttttcca atagctactt tttcctgtcc accgtcatct accaatactg
     3001 cattttgatt aaagatcttt ttgattttca tgcgcgcacc ctttttgaag atttggtttt
     3061 cacgtttgct agaccagttg attgcggcca aacaaaaata gacacgaaaa acctcctagg
     3121 gaaaccatgt tcctcagaga tttttcatgc ctagtatgat ctagtcacat gaaacgaatt
     3181 attgttaacg taatcaattt tatatagaca gttatgtttt gtcaattctt tttttataat
     3241 tatagtaatt tactggaatt atcagaaata aaaggaagga acaaattaag aaacttatgt
     3301 tatttaatca ttggatcttt tgcttaactt attcgttgtt aaaaagctga aagcgaagga
     3361 aaaaaactct acttaactgt atttctccgt taggacatct aaaaatttac gagcagcagg
     3421 agaaagaggc tggtcttttt tccaaatgag attcagtgaa gcttgtaatg gcggatcgag
     3481 tgggatgaat actttttccg tcccataagt attgatgatc ccatctagag ctaatgcacc
     3541 aaaagtcccg ttatcgacca tcaaagaagc attgtacaat aagttatatg ttccgacgat
     3601 cgataactga tctatgctga aacctagcca ttcagctagt tgactgtcca ctgacgtttg
     3661 ccgggaaatg atcaaaggaa cagttttcag ttcctgcgga tggatgactg tttgctttgc
     3721 taaaggatga ttccgatgta tcaaaagtcc ccaacgatct tgggctggca gtcgaaatga
     3781 ctggtatttt tgtttgtttg tcggttcgat tacgagtcca aaatcaagta atccgctatc
     3841 cagtttgtct atgatatcat ctgcattccc gctatataaa tgaaactgga tatcaggatg
     3901 cgccttgatc aacgaatgaa ggctatcaga aatgaaagag attgctttag tttcgcctcc
     3961 gccaatgtag atttctccag agatgattgc gtcatttgtt aaattgttcg ccgttttttc
     4021 taccaacgac aagatttctt ttccttttga ataaagatat cgaccagaat ccgtcaactg
     4081 tatctctcgg gagcctcgag tgaaaagtgt cacaccaagt tcttcttcta aatctttcag
     4141 ttgttttgag agagtaggtt gcgtgatatg cagagatttt gctgctcggc tgatattttt
     4201 ttctctagct actgctaaaa aatacgtcag tatacgaata tccatttttt ccctcctgtt
     4261 tttaaaaaag cataccacta agtatagtta aaaggaatag ttaagtatgt tttataagta
     4321 ttagacatca taagccgtcc gttataaagt aaggataaaa ggagggagca aaatgacaag
     4381 cctcatcgaa aaacaaatcg tcaataaaga gattcgagta acggatccgc tctttgaagt
     4441 gatccatcag atccaagcgg aaaacgaaga gcctttagca gcgctgaata ctgggtacca
     4501 tgaaccagca gatatccgca aacggctaga aaatatcatt agtgacaaag tagatgatac
     4561 ggtgactgtt ctgctgcctt tttatacaga tttcggcaaa catattagta tagggaaaaa
     4621 tgtctttatc aatcgtcaag tgatgttcgt tgacttaggt ggcatctgtt tagaagattc
     4681 agtattgatt ggtcctcgtg tcaacctcat cacggtgaac catctaaccg atccaaaaga
     4741 acgaagaggc ttgtcagtaa agccaatcca catcaagaaa aatgcttgga tcggagcagg
     4801 agcaactatt ctgccaggcg taacgattgg cgagaatgcc attgttgcag cgaatgcaac
     4861 tgttacgaaa gatgtcccag acaataccat cgttgcagga atccctgcca aaatcgtcaa
     4921 acctgtcgaa cggattattt gaacagtaag atgaaataag agatagtgag tataagcaag
     4981 agatttttca aaacaggagg agaatagaat gaatgattta tcagaaaaag tgattgtcat
     5041 catgggcgct tcaagcggaa tcggtgaagc aacaacgaag ctattagcag aaaaaggggc
     5101 taaactagtt atagctgcac gccgagaaga tcgcctaaaa gcaataaaag aatctttacc
     5161 agaagctgag ctatatattc aaacagcaga tgtaagagat tttgcacaag tccaagcagt
     5221 tatcgattta gcgatggaaa agttcggtcg aatcgatgta ttgtacaata atgcaggaat
     5281 catgccaacc gctccattag ttgaaggtca ccgtgatgaa tggcaaaata tgcttgatat
     5341 caacatcatg ggtgtgttaa atggaatttc cgccgtactt ccaatcatgg aaaaacaaaa
     5401 atctgggcat atcatttcaa ctgattctgt ggcgggacat gttgtttatc ctgattccgc
     5461 cgtttattgc ggaacaaaat ttgctgtccg tgcaatcatg gaaggattac gtcaagagca
     5521 acggcaaaat aacatcaaat ctacgatcat ctctccagga gctgttcaaa ctgaattata
     5581 tcaaaccatc agcgacaaaa aggctgctct tgaacttcat gaagcgcaaa aagaatgggg
     5641 attaacatct gaagatatag cttctgctgt agcatttgcg atagaaacgc cagaccggat
     5701 gtctgtcagc aacatgatta tccgtccgac gacacaggaa gtataatcca taacggataa
     5761 tatccagaga gaattacttg ttaaatagtt attcaaacga ccatcaattt aaggcgtgaa
     5821 tggagaagaa tattcttcat ttacgtcttt ttatgcagaa aaagcaaatt tattaaaaag
     5881 aaacaaatgt tatttaaata tggacattga cttgctttta cttttttagt tacactgtta
     5941 tttaggaaaa gtttcataat tcaaaggaga aaaaagatga aagcaaagaa tttattattt
     6001 agcgttattc ttttaggaaa tctagcagtt gccaacacaa cagtgctagc tgaagaatcg
     6061 acagaaactg tcgcaactca gacatcgagt tcttcagaaa caggaattga aaacgataca
     6121 gaaagtactt tgcagtcagc tactgtctca gaaaatacaa tttcaaccga agaaccaaca
     6181 actgaaacaa cagaacaaac acaaaccgaa gacagtacat cagcagaagc tcagaacaat
     6241 ccagagaatg cagaagaaat cacgaaccaa ttgacagatg ggcaaacatt gacacatacc
     6301 catcaagcag atttgtctga attcgaaaac tatactggct atgcttttta tgctcgctca
     6361 tctgctaatt ctcaacaagc gttcattgat agtattgcct caactgcaca aagtttggca
     6421 agtgccaatg acttatatgc atcagtcatg attgctcaag ccatcgttga aagtggctgg
     6481 gggaacagta ctttggcttc tgcccctaat tacaatctat ttgggattaa aggaagctat
     6541 aatggccaat ctgttacgat gcctacttcg gaatatgtga atgggcaatg gattacggtg
     6601 aatgccgctt ttagaaaata tccttcgtac aaagaatcat tgcaagataa cgtaacggta
     6661 ttgaagacga cttcttttca gccaggcgtt tactactatt ccggtgcatg gaaaagcaat
     6721 acgaactctt acaaagatgc tacggcttgg ctaacaggtc gttatgccac tgcgcctaat
     6781 tatggttcta cattgaacaa tgtaatcgaa acctataatc tgacacagta tgatacagca
     6841 ccaacttcaa cttctccgat gtatcgtctg tataaccgtc atacaggaga acatttatat
     6901 acattgaatg ccggagaaaa aaattatctg ccaactgtag gctgggaata tgaagggatt
     6961 gcttggcaag caccaaacag cggacaacct gtctaccgtt tatacaatcc ttacagtgga
     7021 gaccatcatt acacaatggc acaatctgag atcaactctt taacaaaaat cggttggcgt
     7081 tatgaaggtc taagttttta ttctggtggt tccaagccaa tttatcgact atttaatcca
     7141 aatgaaaaaa ctggtaccca tcactatacc ttgagtgcta aggaaagaga ttggctaaca
     7201 ccgatgggct ggagatatga aggaatagga ttttatggtt attaaaaaga ggtagtgaaa
     7261 aagctgccct gcatcaagta gtaagaacag ccaaacaaaa acagattttc atgttttttg
     7321 tttggttgaa cttattaccg cagatgcaag tttttgaaca ccgtttactc aaaaaaggtt
     7381 gtgacaacat cttgtcataa cttttttatt actgtttaag catactgatc caatttctaa
     7441 tattcgacgg gattcttgca aaaggcgatt tcggaaattt ttggcggtgt aaaattatct
     7501 actgcagaag agctatcttc tatagtttga taagaatcaa taatcttggt tttagcgttg
     7561 tgctttcttt atcatttttt ccgttcattc tacaaaaaat ttcttttctc tatacttttc
     7621 tattgagagc gtttttttta ttacacagct gattctttga cttttttctt tttttattct
     7681 attattgaca agtcaatcgt tgacgagtca attattagaa aggaggaaat tatgtcttta
     7741 gaagagatta gtcacctttt atatcaaatc aaattagccg atcaaaaaat cgtccagatc
     7801 tttgaagaaa atatcggtat aagtcttact agatacgaga tcatgatgag actgaaagaa
     7861 gaaaatccat tgctacaaag cagtttgcaa gaaaagttgc agattgatca aggagcagtt
     7921 actcgtcatc tgaaggtatt ggaagcaaat ggctatgttt cgcgtcagag aaatcctcag
     7981 aacaaccggg aagtgtatgt agcgctaaca gaaaaagccc agaagaaaat caagcattgt
     8041 gaaatggacc ataaagatac aggttccgtg ctatccccgg ctttcagcga tgtagaaatg
     8101 aaacaatttc taacactttt agataaattg aatacccaac tttgcaatat aacaacagaa
     8161 acgaaagagg ttaataataa tggaacaaaa aaatgatttt tcaacaatta tgactggtag
     8221 aagatccgtt cgcgtatatg atgaaacata caaaatccca cacgaagaaa tgctggaaat
     8281 gatcaaagaa gcgacgactg caccttcttc tgtcaatatg aaaccatggc gctttgtggt
     8341 cgctgaaagc gaggaagcaa aagaaatctt gcgtcctttg atccgtttca atacgagaca
     8401 aaatgatact tcttctgcaa tggtcatgat ttttggcgat atgcaatgtt acgaatatgg
     8461 cgaagaaatc tacaaccaag cttacgaatc agggaagatg ccaaaagaag tcagagatca
     8521 acaattagct gcgatcattc cttattacaa gagtttttca agagaagaaa tgaacgatgt
     8581 agtcaaagtc gattctagct tagcggctat gcaattcatg ttagttgcgc gtgatcatgg
     8641 ctatgaaaca aatccaattg gtggattcga agcagatcag ttggcagagg catttggttt
     8701 ggataaagac cgttatgttc cagttattat tttatcaata ggaaaagcgg tagaagaagg
     8761 ttacgagtct gttcgtttag caccagaaaa gatcactatg ttcgaataaa aggttttcag
     8821 ttactattca gtagctattg ctttttttga atcgtgtgtg gtaacctaat aaaaaaggag
     8881 gtcggaaaaa tgactgcatc gatgaaatta cgtttcgaac ttttgaataa caactaaata
     8941 aattcaaaag acatgtataa acaacaattt catggatgga gtatgtccat tttttcgaac
     9001 gacagtagaa taggtgaaag ctatgacaga tttggtgatt tagtcatagt tatttcgttc
     9061 agagtaggaa aagatgtgat gcagtttttt tgcaaacaga tttttctgct cttttttagt
     9121 atcgataatt gttgttttac atgaaatagg agtgtaaaac atatggaaaa tttagcagta
     9181 aatataacaa atctacaagt cagctttggc aaccagctag aactatctat tgattctctt
     9241 cgtgtctatc agcaagatcg tatagggatc ataggagaaa atggtgtcgg taaatcgact
     9301 ttgctcaaac taatagccgg tgaacttttt cccgatcatg ggaaaatcca aacagagatc
     9361 accttcaact acctgcctca attaacctat ctcgctgaag caaaggacct aaatttggaa
     9421 ttagccagtc atttccagtt aagactggaa gaaacttcag agcggaaatg gagcggaggg
     9481 gaagaacgaa agatcgagtt gatacgtctt ctttcttctt atgaacaagg gatgcttcta
     9541 gacgagccaa caacccatct agatagaaaa agtattgatc gactgattga agagcttcgt
     9601 tattattatg gtacgctggt ttttgttagt catgatcgct attttctaga tgagctagca
     9661 tcgaaaatct gggaagtaaa agacggagaa atccgagagt tttcggggaa ttatagtgcc
     9721 tatctcactc aaaaggaatt ggagaaaaag actcagctac gagaagcaga gtcgatcatg
     9781 aaagagaaaa aacgattgga aaaatcgatc caagaaaaga aaaaacaagc ggaaaagttg
     9841 gaaaaagtgt ccagtaaaaa gaaaaagcaa caaatcagac tggatcggtt gtcttcctct
     9901 aaacaaaaag acagtgtaca aaaagccatc caaaagaatg cgaaaacatt agagagaaga
     9961 ctccaaaaaa taggagaaac aaccaagccg cagcagatga aacaaatccg ttttccagta
    10021 ccaaaatctc ttgaactcca cagccgctat cccatcatgg gacaaaatgt ccaattggaa
    10081 agaagtggga gaacattact ggtaaatggt gattttcagt tttctttagg taaaaaaatc
    10141 gcgattgtcg gtgaaaatgg ttcaggtaag acaaccttat tagaacatat ccgcaaacaa
    10201 ggagaaggaa tccttctctc tccgaaagta agctttcaag tatatcagca aaagggttat
    10261 caaatgacat ctgaagaatc catcattcgt tttgtcatga gacaaacaga gttttcagaa
    10321 tcgcttgtcc gtagtttgct gaatcactta gggtttgctc aggaaacttt gacgaaaccg
    10381 ttatgtacat taagtggggg agaagcgacc cgtctgacga ttgctttgct ttttactaag
    10441 ccaagtaatg tgttgctgtt agatgaaccg actaatttta ttgatatggc aacgatcgaa
    10501 gctttagaga agctaatgca aatatatccg ggaacgattt tgtttacttc tcatgattct
    10561 tactttgtcg agcgtacggc tgatgaagtt tatgaaataa aagggcagaa aataaaaaaa
    10621 gtacttacga gaaattttta aatgtgctac gatgattttc gtgacaaatt taaaggagtg
    10681 gtacagattg cctacaaata aaaaagaagg aataattttt acaacaatga tgtgtttttt
    10741 gatggtcttg gggatgagcg cgtataattt atggctgcat caagatcttc atttatcaag
    10801 tttgttgatt ggtctagtgc ctggattcgt ggtggctttc attttggatg tcttcatcat
    10861 cggtgtgctg gcgaaaaaaa ttgcctttaa actaccagta aatccagaaa gcaaggtcca
    10921 aatgatctta actatttcat gcttgatggt tttaggaatg gtgacatgta tgtctttatt
    10981 cggtgtgatc atggaacaag gaatacctga tcaattattc tctgcttata tgaatgcttg
    11041 gaaaatgaat gtgattgccg ctttaccgtt gcagttgatc atcgttggcc cgctttcaag
    11101 aagtgtgcta aaagcaattc aaagtaatga atgatggaac actgttattt gaaaaaaggc
    11161 cttggcatgc tttttgtcat agtctcttga atgcagttct agaaaaatag tgatccgaac
    11221 gataggtttt ggcctgtcgt tcggatcgct ttttcttttc caactgtatt gctcttttat
    11281 tgaagataat ttgctcttca atagatattt ctagaaaggt tctgtttatc ccttactcaa
    11341 aaaactgctg catttgttct cttacttgct gacaccattg gagttcctgc tgacaaagca
    11401 taattctgcg ctggatcaca acataacgtc caaattcttg tttcttgtct agcgagaggt
    11461 cattcaattt tttttgattt ttttgcatga ttttgttcag ctgttgttct ctagtgaata
    11521 gcagtccagt agctgttgtt ttatcgagcg tcgaaaaagc atagattttt gctagaaatt
    11581 catctttttg cgtaggagcg ggagtttcct ctttgatcca ttcctctaca agagcacgtc
    11641 cttcatcagt caagtggtag atcttttttt gagagtgctc gtctttgtcc aaaacggtca
    11701 cataaccctg ctggtctaac ttggcaagcg ttgtatagat ctgactgtga tgggcttccc
    11761 aaaaaattgt gatcagctgc ttcaattcat acccagaacg tggtttggat aaaagcacgc
    11821 ataataatat ataggctaaa gtattcataa aaatcctacc tctcgtctta ttttctttat
    11881 tatatcacag taaagtttcc acgttttaca tgtgcatatt gacatgtaaa ataaatcatg
    11941 tatgatgcta atagagagaa agggatggaa atattggaga atcaaataga tatacaagaa
    12001 caaacaaatg aagaaacaaa ggggaaaagt tcagcgctag ttgtagcagc tacctgtatg
    12061 atcgctttta tgggagtggg tctagtagac ccaatcttaa aaacgattgc tgcacagtta
    12121 aacgctacgc cagctgaaac gaccctgtta tttactagtt acatgttagt cactggtgtg
    12181 gtgatgctat ttacaggttt cttatctagt cggatcggtt tgaaaaaaac attgatgaca
    12241 ggattgttta tcatcgttgt attcgctggt cttggcggat tatctgggaa tatcggaatg
    12301 ctgattgggt tacgtgccgg ctggggtgtc gggaatgctt tatttgtctc tactgcttta
    12361 gcaacgatcg tcagtatttt agttggtaac acagaaaaag caatcatgat gtatgaagca
    12421 gcacttggct gcggaatggc tgttggccct ctagtcggtg gggtcttggg cagtggttca
    12481 tggcgttatc cattttttgg ggtagcattt ttgatgtttt gcggttttat agccctagcg
    12541 gtacttttac ctgaaactga gaaaccaaag agaaaagtag gcctttggga aggtgtcaaa
    12601 gcgttaagca atcataaact tcgttcagtt ggactaatcg cattgttata taatttcgga
    12661 ttctttactt tacttgcgta tgcgccattt ttattgattg gctattctga attagaagtc
    12721 ggttttgtat tttttggctg gggtgtgctt ctggctattg cttcgatttt cgttgcaccg
    12781 gctcttgagc ggaagtttac gacttatcgg acaattttag cagcatttgt cttattcatt
    12841 atttgtttgc tgttattagg agctggaact tctatgccag tccttgtacc gatcagcatt
    12901 gtgttagcag gatttttcca aggaattatc aatacactgt tgacgacgat tgctatggaa
    12961 ataccaggtc tgcaacgaaa cgttgcttcg tccagctata gttttgtccg ctttttcggt
    13021 ggagctttag caccatttat tgcaggtgtg attggtgaac atattggtga aagctatgcg
    13081 ttttattttg caggtgccat tgttcttatc agtgtatgtt gtatcattta tcaccgtaat
    13141 tattttgcta caaatgaaga agaataagaa atcagaggtt gtgaaaaagc tgtcctgcat
    13201 caagtcataa gaa
//
LOCUS       DAZKKW010000339         3301 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.guided.164, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000339 DAZKKW010000000
VERSION     DAZKKW010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3301
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.guided.164"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..163
                     /locus_tag="ACG8PO_003126"
     CDS             <1..163
                     /locus_tag="ACG8PO_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HFQ0208352.1"
                     /translation="KYTEKVNHIFEVLGISLFECYDMTEKLYNEVNEIVSEINNFLNE
                     ESSDERKQI"
     gene            144..878
                     /locus_tag="ACG8PO_003127"
     CDS             144..878
                     /locus_tag="ACG8PO_003127"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008425 -
                     2-polyprenyl-6-methoxy-1,4-benzoquinone methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0102208 - 2-polyprenyl-6-hydroxyphenol
                     methylase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFQ0208353.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            911..1819
                     /locus_tag="ACG8PO_003128"
     CDS             911..1819
                     /locus_tag="ACG8PO_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HFQ0208354.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            1816..2358
                     /gene="sat4"
                     /locus_tag="ACG8PO_003129"
     CDS             1816..2358
                     /gene="sat4"
                     /locus_tag="ACG8PO_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HFQ0208355.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
     gene            2451..3245
                     /locus_tag="ACG8PO_003130"
                     /note="aph(3')-IIIa"
     CDS             2451..3245
                     /locus_tag="ACG8PO_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HFQ0208356.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
BASE COUNT         1127 a          498 c          771 g          905 t
ORIGIN      
        1 aaaatatacc gagaaggtaa atcatatttt tgaagtactc ggtatctcac tttttgaatg
       61 ctacgacatg accgagaagc tttataatga agtgaatgaa attgtatcgg agataaataa
      121 ctttttaaac gaggagagtt cagatgaaag aaaacaaata tgatgataat atattttttc
      181 aaaaatacag tcaaatgagt cgctcgcaga aaggactggc tggtgcggga gaatgggaga
      241 ctttgaaaaa gatgctacct gattttaagg gtaagcgtgt gcttgattta ggatgcggct
      301 atggatggca ctgtatatat gcgatggaaa acggtgcttc ctctgtagta ggtgttgata
      361 tttctcataa aatgctcgaa gtagcaaaag gaaaaaccca ttttccacag attgaatatg
      421 aatgctgtgc catagaagat gtggatttcc cagaggagag ctttgatgta atactaagtt
      481 cgcttgcgtt tcattatgta gcagactatg agaatttaat aaaaaagata tataggatgc
      541 tgaaggctgg tggcaattta gtttttacag ttgaacatcc tgtttttact gctcatggaa
      601 cacaagactg gtattataac gaaaaaggag aaatactgca tttcccggtg gacaattatt
      661 attatgaggg caaacggaca gctatgtttt tggaagaaaa ggttacaaaa tatcatagaa
      721 cactgaccac atatctaaat acactgcttt caaatagttt tataataaat cagattgtgg
      781 agccacagcc gccagagaac atgatggata ttccggggat ggcggatgaa atgcgacgcc
      841 caatgatgct gattgtatcg gcaaaaaaga agatgtaata atatagaaaa aataaacgag
      901 gagtatgtaa atgagatcag aaaaagaaat gatggattta gtactttctt tagcagaaca
      961 ggatgaacgt attcgaattg tgacccttga ggggtcacgc gcaaatatta atatacctaa
     1021 agatgaattt caggattatg atattacata ttttgtaagt gatatagaac cgtttatatc
     1081 taatgatgac tggcttaatc aatttgggaa tataataatg atgcaaaagc cggaggatat
     1141 ggaattattc ccacctgaag aaaagggatt ttcctatctt atgctatttg atgattacaa
     1201 taaaattgat cttaccttat tgcccttgga agagttagat aattacctaa agggcgataa
     1261 attaataaag gttctaattg ataaagattg tagaattaaa agggacatag ttccgactga
     1321 tatagattat catgtaagaa agccaagcgc aagggagtat gatgattgct gcaatgaatt
     1381 ttggaatgta acaccttatg ttattaaagg attgtgccgt aaggaaattt tatttgctat
     1441 tgatcatttt aatcagattg ttcgccatga gctgctgaga atgatatcat ggaaggtcgg
     1501 catcgaaaca ggctttaaat taagtgtagg caagaactat aagtttattg aaaggtatat
     1561 atccgaggat ttgtgggaga aacttttgtc cacctaccgg atggattcct atgaaaacat
     1621 atgggaagca ttatttctat gccatcaatt gttcagggcg gtatccggtg aggtggcgga
     1681 aaggcttcat tatgcctatc cggagtatga taggaatata acaaaatata ccagggacat
     1741 gtataaaaaa tacactggta aaaccggctg cctggatagc acatatgccg ctgatataga
     1801 agagaggcgg gaacagtgat tacagaaatg aaagcagggc acctgaaaga tatcgataaa
     1861 cccagcgaac catttgaggt gataggtaag attataccga ggtatgaaaa cgagaattgg
     1921 acctttacag aattactcta tgaagcgcca tatttaaaaa gctaccaaga cgaagaggat
     1981 gaagaggatg aggaggcaga ttgccttgaa tatattgaca atactgataa gataatatat
     2041 ctttactacc aagacgataa atgcgtcgga aaagttaaac tgcgaaaaaa ttggaaccgg
     2101 tacgcttata tagaagatat cgccgtatgt aaggatttca gggggcaagg cataggcagc
     2161 gcgcttatca atatatctat agaatgggca aagcataaaa acttgcatgg actaatgctt
     2221 gaaacccagg acaataacct tatagcttgt aaattctatc ataattgtgg tttcaaaatc
     2281 ggctccgtcg atactatgtt atacgccaac tttgaaaaca actttgaaaa agctgttttc
     2341 tggtatttaa ggttttagaa tgcaaggaac agtgaattgg agttcgtctt gttataatta
     2401 gcttcttggg gtatctttaa atactgtaga aaagaggaag gaaataataa atggctaaaa
     2461 tgagaatatc accggaattg aaaaaactga tcgaaaaata ccgctgcgta aaagatacgg
     2521 aaggaatgtc tcctgctaag gtatataagc tggtgggaga aaatgaaaac ctatatttaa
     2581 aaatgacgga cagccggtat aaagggacca cctatgatgt ggaacgggaa aaggacatga
     2641 tgctatggct ggaaggaaag ctgcctgttc caaaggtcct gcactttgaa cggcatgatg
     2701 gctggagcaa tctgctcatg agtgaggccg atggcgtcct ttgctcggaa gagtatgaag
     2761 atgaacaaag ccctgaaaag attatcgagc tgtatgcgga gtgcatcagg ctctttcact
     2821 ccatcgacat atcggattgt ccctatacga atagcttaga cagccgatta gccgaattgg
     2881 attacttact gaataacgat ctggccgatg tggattgcga aaactgggaa gaagacactc
     2941 catttaaaga tccgcgcgag ctgtatgatt ttttaaagac ggaaaagccc gaagaggaac
     3001 ttgtcttttc ccacggcgac ctgggagaca gcaacatctt tgtgaaagat ggcaaagtaa
     3061 gtggctttat tgatcttggg agaagcggca gggcggacaa gtggtatgac attgccttct
     3121 gcgtccggtc gatcagggag gatatcgggg aagaacagta tgtcgagcta ttttttgact
     3181 tactggggat caagcctgat tgggagaaaa taaaatatta tattttactg gatgaattgt
     3241 tttagtacct agatttagat gtctaaaaag ctttaactac aagcttttta gacatctaat
     3301 c
//
LOCUS       DAZKKW010000340         3279 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.guided.165, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000340 DAZKKW010000000
VERSION     DAZKKW010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3279
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.guided.165"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            245..1213
                     /locus_tag="ACG8PO_003131"
     CDS             245..1213
                     /locus_tag="ACG8PO_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HFQ0208357.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            1206..2237
                     /gene="vanA"
                     /locus_tag="ACG8PO_003132"
     CDS             1206..2237
                     /gene="vanA"
                     /locus_tag="ACG8PO_003132"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HFQ0208358.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALAVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            2243..2851
                     /locus_tag="ACG8PO_003133"
     CDS             2243..2851
                     /locus_tag="ACG8PO_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HFQ0208359.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPNRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT          973 a          629 c          792 g          885 t
ORIGIN      
        1 atgctggtgt catccaccaa catcaaaaag tatagagcca taatttttta ggaaaatctc
       61 aaggttatct ttactttttc ttaggaaatt aacaatttaa tattaagaaa cggctcgttc
      121 ttacacggta gacttaatac cgtaagaacg agccgttttc gttcttcaga gaaagatttg
      181 acaagattac cattggcatc cccgttttat ttggtgcctt tcacagaaag ggttggtctt
      241 aattatgaat aacatcggca ttactgttta tggatgtgag caggatgagg cagatgcatt
      301 ccatgctctt tcgcctcgct ttggcgttat ggcaacgata attaacgcca acgtgtcgga
      361 atccaacgcc aaatccgcgc ctttcaatca atgtatcagt gtgggacata aatcagagat
      421 ttccgcctct attcttcttg cgctgaagag agccggtgtg aaatatattt ctacccgaag
      481 catcggctgc aatcatatag atacaactgc tgctaagaga atgggcatca ctgtcgacaa
      541 tgtggcgtac tcgccggata gcgttgccga ttatactatg atgctaattc ttatggcagt
      601 acgcaacgta aaatcgattg tgcgctctgt ggaaaaacat gatttcaggt tggacagcga
      661 ccgtggcaag gtactcagcg acatgacagt tggtgtggtg ggaacgggcc agataggcaa
      721 agcggttatt gagcggctgc gaggatttgg atgtaaagtg ttggcttata gtcgcagccg
      781 aagtatagag gtaaactatg taccgtttga tgagttgctg caaaatagcg atatcgttac
      841 gcttcatgtg ccgctcaata cggatacgca ctatattatc agccacgaac aaatacagag
      901 aatgaagcaa ggagcatttc ttatcaatac tgggcgcggt ccacttgtag atacctatga
      961 gttggttaaa gcattagaaa acgggaaact gggcggtgcc gcattggatg tattggaagg
     1021 agaggaagag tttttctact ctgattgcac ccaaaaacca attgataatc aatttttact
     1081 taaacttcaa agaatgccta acgtgataat cacaccgcat acggcctatt ataccgagca
     1141 agcgttgcgt gataccgttg aaaaaaccat taaaaactgt ttggattttg aaaggagaca
     1201 ggagcatgaa tagaataaaa gttgcaatac tgtttggggg ttgctcagag gagcatgacg
     1261 tatcggtaaa atctgcaata gagatagccg ctaacattaa taaagaaaaa tacgagccgt
     1321 tatacattgg aattacgaaa tctggtgtat ggaaaatgtg cgaaaaacct tgcgcggaat
     1381 gggaaaacga caattgctat tcagctgtac tctcgccgga taaaaaaatg cacggattac
     1441 ttgttaaaaa gaaccatgaa tatgaaatca accatgttga tgtagcattt tcagctttgc
     1501 atggcaagtc aggtgaagat ggatccatac aaggtctgtt tgaattgtcc ggtatccctt
     1561 ttgtaggctg cgatattcaa agctcagcaa tttgtatgga caaatcgttg acatacatcg
     1621 ttgcgaaaaa tgctgggata gctactcccg ccttttgggt tattaataaa gatgataggc
     1681 cggtggcagc tacgtttacc tatcctgttt ttgttaagcc ggcgcgttca ggctcatcct
     1741 tcggtgtgaa aaaagtcaat agcgcggacg aattggacta cgcaattgaa tcggcaagac
     1801 aatatgacag caaaatctta attgagcagg ctgtttcggg ctgtgaggtc ggttgtgcgg
     1861 tattgggaaa cagtgccgcg ttagctgttg gcgaggtgga ccaaatcagg ctgcagtacg
     1921 gaatctttcg tattcatcag gaagtcgagc cggaaaaagg ctctgaaaac gcagttataa
     1981 ccgttcccgc agacctttca gcagaggagc gaggacggat acaggaaacg gcaaaaaaaa
     2041 tatataaagc gctcggctgt agaggtctag cccgtgtgga tatgttttta caagataacg
     2101 gccgcattgt actgaacgaa gtcaatactc tgcccggttt cacgtcatac agtcgttatc
     2161 cccgtatgat ggccgctgca ggtattgcac ttcccgaact gattgaccgc ttgatcgtat
     2221 tagcgttaaa ggggtgataa gcatggaaat aggatttact tttttagatg aaatagtaca
     2281 cggtgttcgt tgggacgcta aatatgccac ttgggataat ttcaccggaa aaccggttga
     2341 cggttatgaa gtaaatcgca ttgtagggac atacgagttg gctgaatcgc ttttgaaggc
     2401 aaaagaactg gctgctaccc aagggtacgg attgcttcta tgggacggtt accgtcctaa
     2461 tcgtgctgta aactgtttta tgcaatgggc tgcacagccg gaaaataacc tgacaaagga
     2521 aagttattat cccaatattg accgaactga gatgatttca aaaggatacg tggcttcaaa
     2581 atcaagccat agccgcggca gtgccattga tcttacgctt tatcgattag acacgggtga
     2641 gcttgtacca atggggagcc gatttgattt tatggatgaa cgctctcatc atgcggcaaa
     2701 tggaatatca tgcaatgaag cgcaaaatcg cagacgtttg cgctccatca tggaaaacag
     2761 tgggtttgaa gcatatagcc tcgaatggtg gcactatgta ttaagagacg aaccataccc
     2821 caatagctat tttgatttcc ccgttaaata aacttttaac cgttgcacgg acaaactata
     2881 taagctaact ctttcggcag gaaacccgac gtatgtaact ggttcttagg gaatttatat
     2941 atagtagata gtattgaaga tgtaaggcag agcgatattg cggtcattat ctgcgtgcgc
     3001 tgcggcaaga tagcctgata ataagactga tcgcatagag gggtggtatt tcacaccgcc
     3061 cattgtcaac aggcagttca gcctcgttaa attcagcatg ggtatcactt atgaaaattc
     3121 atctacattg gtgataatag taaatccagt agggcgaaat aattgactgt aatttacggg
     3181 gcaaaacggc acaatctcaa acgagattgt gccgtttaag gggaagattc tagaaatatt
     3241 tcggctctat actttttgat gttggtgaac ttcaccaac
//
LOCUS       DAZKKW010000341         2291 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.guided.192, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000341 DAZKKW010000000
VERSION     DAZKKW010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2291
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.guided.192"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            217..566
                     /locus_tag="ACG8PO_003134"
                     /pseudo
     CDS             217..566
                     /locus_tag="ACG8PO_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741963.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(838..1128)
                     /locus_tag="ACG8PO_003135"
     CDS             complement(838..1128)
                     /locus_tag="ACG8PO_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0208360.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(1336..2028)
                     /locus_tag="ACG8PO_003136"
     CDS             complement(1336..2028)
                     /locus_tag="ACG8PO_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFQ0208361.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQAITDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT          657 a          425 c          329 g          880 t
ORIGIN      
        1 aaactacgtc atccgctctc atagtcggca tatacatatt aaagagtaaa aagatacccc
       61 aaatccctat tagtaacaag atcagttctt tgttattcgt tatttttcta ttatgtttgt
      121 tcatttttat actccttctt atatttccta atagagtata acatagcttt aagcattact
      181 taaagataac ttaaaagtta tcttttaaat acttaattgt taaatacctt atttgatgca
      241 aaagaaatct acgatagtca acaaaaaaat tttcattata tccagaaaaa tttttcttag
      301 ttaaggacct atggattgca gtaatgcaga attcgtcgaa taaactgcca aagacataca
      361 atcatattgc ttttaaaatt gacgaacaag atatagattc gtttgtctca aaaatccaaa
      421 tgttaggttt aactgtcgaa ccaggacgtt ctcgtgtgaa aggcgaggct tcttcaattt
      481 acttttacga ctatgataat cacctcttcg agttacatac cggtactctc caggaacgtt
      541 taaaatccta caattccact acctgagact agtaatgact agtctctttt tatggcctct
      601 gatcccccca ttgagcgaat actaataggg cttcgaattg atctgttcaa tctttaagca
      661 atatccctca aatgagtttg gaaagtttag tgattcatca caaatactta aatacatgta
      721 gttatatata gattgaggtt cttgtgagcg tatgctcgtg tgggttcaaa tcccatcgtc
      781 tgcatccatc tttccaaaac aagcggctag gacatttttg tctcagccgc ttgttttcta
      841 gtttattctt tcatcttgtc ggaccttttt tcttgggtat agacttttct catattctac
      901 cacaattttg ggtttcccta acacatgata ggatctcaca acaaattgct ttctattatt
      961 ataataacct gtaacgacta acttcatccc ttcgtctact tcagctagga agttcaaact
     1021 atgggaagca atcaaacaat tgaccccatt taaactgaaa cggatcaacg gtctggcact
     1081 aacttgcaaa gtacggatct tatctactgt tccatatttt ttctccataa ccttcacctc
     1141 aataccatta tacgaacata cattcccttt gtaaagaaga aactctgttt tatctttttg
     1201 actttttgtc tattaccttt cttaaaatca tattggcact tcctgcgaag atgaaggaca
     1261 tgtactaaaa aaaagaggca gcagtataga ttactgttgc ttctttacta atttaggttt
     1321 acttgttaag aaagattatt tacgcaaatg ttcttcaaca atttgctgag atttatcagc
     1381 tgtatcttcc acttcatctt ttgctttttc taacttttga atatattgtt tcaagcttga
     1441 gttttcagca gttgcttcgt ctaatttctt ttgtgtatct gttaactgag tttctacatt
     1501 tgttttttct tgtgtcagtg ttgcgatttg ttgattcaaa ccatttacag tattttgtaa
     1561 ttctgaaagt tttgcttgtg cttcttgtag tcttgctgtc caatctgcat tcagatgatt
     1621 gatttcatca tttttttggt tgatctcatc tagtttgaca ttgatttcat tttgtttgct
     1681 ttgtagttgt gcatccttgc tgactaattg actttctaag ttttgttttt ctgctgccga
     1741 ttgatttttt tggttttcta attcatttac ttttgtttgg tattcttgga catctgcctt
     1801 tgaatcttgc aatgtctgtt ctttttgtgc tagtcgagcg gtaagatctg taatcgcttg
     1861 ctctttatct gtcaatgtat gatcaagctg tgtcagattc tcattgattg tatcaatatc
     1921 tgtctctcca ccccacaaaa taatttcatt tgcgtaccgc gtgcctgtgt agccaattag
     1981 acatagaaat gcaacgacaa tcccaccagt aataaatttc tttttcattt gcatagctcc
     2041 ttaatccaat acttttacat gctaaaagta agtgatctca aacacaatgt caagcgtttt
     2101 taaattacat atgtaatttt aatataaata ctatcaaata ctcatttaat cctccttttt
     2161 tatctattta aaaaatcttt tttatagaga aaaatgcagg ttaatacttt cttaaataaa
     2221 tatttatggt gaacaaaaac aaaaaagcaa gttttattaa acgtaaccct tttcatttca
     2281 tagattactg t
//
LOCUS       DAZKKW010000342         2189 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.guided.196, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000342 DAZKKW010000000
VERSION     DAZKKW010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2189
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.guided.196"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            310..1005
                     /gene="vanR-A"
                     /locus_tag="ACG8PO_003137"
     CDS             310..1005
                     /gene="vanR-A"
                     /locus_tag="ACG8PO_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-A"
                     /protein_id="HFQ0208362.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            983..2137
                     /locus_tag="ACG8PO_003138"
     CDS             983..2137
                     /locus_tag="ACG8PO_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS-A"
                     /protein_id="HFQ0208363.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
BASE COUNT          734 a          405 c          465 g          585 t
ORIGIN      
        1 tatttgatag tagatttaaa actttgcaac agaaccgaaa ttactaatgt atctagggct
       61 tcattataca ggaaattatc agaagtgaat aattagccat tctgtattcc gctaatgggc
      121 aatattttta aagaagaaaa ggaaactata aaatattaac agcctcctag cgatgccgaa
      181 aagccctttg ataaaaaaag aatcatcatc ttaagaaatt cttagtcatt tattatgtaa
      241 atgcttataa attcggccct ataatctgat aaattattaa gggcaaactt atgtgaaagg
      301 gtgataacta tgagcgataa aatacttatt gtggatgatg aacatgaaat tgccgatttg
      361 gttgaattat acttaaaaaa cgagaattat acggttttca aatactatac cgccaaagaa
      421 gcattggaat gtatagacaa gtctgagatt gaccttgcca tattggacat catgcttccc
      481 ggcacaagcg gccttactat ctgtcaaaaa ataagggaca agcacaccta tccgattatc
      541 atgctgaccg ggaaagatac agaggtagat aaaattacag ggttaacaat cggcgcggat
      601 gattatataa cgaagccctt tcgcccactg gagttaattg ctcgggtaaa ggcccagttg
      661 cgccgataca aaaaattcag tggagtaaag gagcagaacg aaaatgttat cgtccactcc
      721 ggccttgtca ttaatgttaa cacccatgag tgttatctga acgagaagca gttatccctt
      781 actcccaccg agttttcaat actgcgaatc ctctgtgaaa acaaggggaa tgtggttagc
      841 tccgagctgc tatttcatga gatatggggc gacgaatatt tcagcaagag caacaacacc
      901 atcaccgtgc atatccggca tttgcgcgaa aaaatgaacg acaccattga taatccgaaa
      961 tatataaaaa cggtatgggg ggttggttat aaaattgaaa aataaaaaaa acgactattc
     1021 caaactagaa cgaaaacttt acatgtatat cgttgcaatt gttgtggtag caattgtatt
     1081 cgtgttgtat attcgttcaa tgatccgagg gaaacttggg gattggatct taagtatttt
     1141 ggaaaacaaa tatgacttaa atcacctgga cgcgatgaaa ttatatcaat attccatacg
     1201 gaacaatata gatatcttta tttatgtggc gattgtcatt agtattctta ttctatgtcg
     1261 cgtcatgctt tcaaaattcg caaaatactt tgacgagata aataccggca ttgatgtact
     1321 tattcagaac gaagataaac aaattgagct ttctgcggaa atggatgtta tggaacaaaa
     1381 gctcaacaca ttaaaacgga ctctggaaaa gcgagagcag gatgcaaagc tggccgaaca
     1441 aagaaaaaat gacgttgtta tgtacttggc gcacgatatt aaaacgcccc ttacatccat
     1501 tatcggttat ttgagcctgc ttgacgaggc tccagacatg ccggtagatc aaaaggcaaa
     1561 gtatgtgcat atcacgttgg acaaagcgta tcgactcgaa cagctaatcg acgagttttt
     1621 tgagattaca cggtataacc tacaaacgat aacgctaaca aaaacgcaca tagacctata
     1681 ctatatgctg gtgcagatga ccgatgaatt ttatcctcag ctttccgcac atggaaaaca
     1741 ggcggttatt cacgcccccg aggatctgac cgtgtccggc gaccctgata aactcgcgag
     1801 agtctttaac aacattttga aaaacgccgc tgcatacagt gaggataaca gcatcattga
     1861 cattaccgcg ggcctctccg gggatgtggt gtcaatcgaa ttcaagaaca ctggaagcat
     1921 cccaaaagat aagctagctg ccatatttga aaagttctat aggctggaca atgctcgttc
     1981 ttccgatacg ggtggcgcgg gacttggatt ggcgattgca aaagaaatta ttgttcagca
     2041 tggagggcag atttacgcgg aaagcaatga taactatacg acgtttaggg tagagcttcc
     2101 agcgatgcca gacttggttg ataaaaggag gtcctaagag atgtatataa ttttggctct
     2161 ttgtcaaatg atgttggtaa agagaaatc
//
LOCUS       DAZKKW010000343         2053 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.guided.202, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000343 DAZKKW010000000
VERSION     DAZKKW010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2053
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.guided.202"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            183..920
                     /gene="erm(B)"
                     /locus_tag="ACG8PO_003139"
     CDS             183..920
                     /gene="erm(B)"
                     /locus_tag="ACG8PO_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HFQ0208364.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNIRVTLIHQDILQFQFPNKQRYKIVGSIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            1090..1350
                     /locus_tag="ACG8PO_003140"
                     /pseudo
     CDS             1090..1350
                     /locus_tag="ACG8PO_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
     gene            1513..>2053
                     /locus_tag="ACG8PO_003141"
     CDS             1513..>2053
                     /locus_tag="ACG8PO_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFQ0208365.1"
                     /translation="MYQNYTINQLCLPIDLEIKLEENDFAHAIVQFVDSIPDEVFLPY
                     YQSMGRPQYHPRMLLSIILCAYIQGVYSGRQIQNMLIDSIRMRYLSQEQFPNFRTINR
                     FRVHPIMNDILDHSFVQFRELLVQSGLISGSALYIDGTKIEADANKYSFVWKKSILKY
                     KDSLDQKALENYQQMVETKI"
BASE COUNT          741 a          346 c          342 g          624 t
ORIGIN      
        1 agataaaaca tctactgttt tgaaacagac taaaaacagt gattacgcag ataaataaat
       61 acgttagatt aattcctacc agtgactaat cttatgactt tttaaacaga taactaaaat
      121 tacaaacaaa tcgtttaact tctgtattta tttacagatg taataacttc aggagtgatt
      181 acatgaacaa aaatataaaa tattctcaaa actttttaac gagtgaaaaa gtactcaacc
      241 aaataataaa acaattgaat ttaaaagaaa ccgataccgt ttacgaaatt ggaacaggta
      301 aagggcattt aacgacgaaa ctggctaaaa taagtaaaca ggtaacgtct attgaattag
      361 acagtcatct attcaactta tcgtcagaaa aattaaaact gaacattcgt gtcactttaa
      421 ttcaccaaga tattctacag tttcaattcc ctaacaaaca gaggtataaa attgttggga
      481 gtattcctta ccatttaagc acacaaatta ttaaaaaagt ggtttttgaa agccatgcgt
      541 ctgacatcta tctgattgtt gaagaaggat tctacaagcg taccttggat attcaccgaa
      601 cactagggtt gctcttgcac actcaagtct cgattcagca attgcttaag ctgccagcgg
      661 aatgctttca tcctaaacca aaagtaaaca gtgtcttaat aaaacttacc cgccatacca
      721 cagatgttcc agataaatat tggaagctat atacgtactt tgtttcaaaa tgggtcaatc
      781 gagaatatcg tcaactgttt actaaaaatc agtttcatca agcaatgaaa cacgccaaag
      841 taaacaattt aagtaccgtt acttatgagc aagtattgtc tatttttaat agttatctat
      901 tatttaacgg gaggaaataa ttctatgagt cgctttttta aatttggaaa gttacacgtt
      961 actaaaggga atggagataa attattagat atactactga cagcttccaa gaagctaaag
     1021 aggtccctag cgcctacggg gaatttgtat cgataagggg tacaaattcc cactaagcgc
     1081 tcgggacccc ttgtaggaaa atgtcctaag tgtggcaaca atattgtatt aaaaaaatcg
     1141 ttttatggtt gttcaaatta tcctgaatgt aagtttactt tagctgaaca ttttagaaag
     1201 aaaaaactaa ccaaaacgaa tgtaaaagaa ttactggagg gaaaagaaac cctggtaaaa
     1261 ggaatcaaaa acaaagagaa aaagccctac aatgccgttg taaaaattgg ggaaaaggga
     1321 tatattgatt ttatctcttt ttcaaaataa acatggggct gacccaaaag tagctcctat
     1381 atgaaaaata gaggtctttc catttagata gtagtggaaa gacctctatt tatcttttga
     1441 aagtgaatac aaaaataaag cacactctgc tataatatag gtgattaatc catttgagag
     1501 aggtgcttta tcatgtatca aaattatacc ataaaccaac tttgtttgcc tatagatctt
     1561 gaaattaaat tagaagaaaa tgattttgct catgccattg tgcagtttgt tgactctatt
     1621 cccgatgaag tctttcttcc ttattatcaa tctatgggta gaccccaata tcatcctcgt
     1681 atgctacttt ccattattct ttgtgcctat attcaagggg tttattcggg ccgccaaata
     1741 caaaatatgc tgatagatag tattcggatg cgctacttat ctcaagagca attccctaac
     1801 tttcgtacta ttaatcgttt tcgcgttcat ccgattatga acgatattct agatcattca
     1861 tttgttcaat ttcgtgagct actggttcaa agtggcttaa tcagtggcag tgctctatac
     1921 attgatggta caaaaattga agcggatgcg aataaatact ctttcgtttg gaaaaaaagt
     1981 attttaaagt ataaggatag ccttgatcaa aaagcccttg aaaactacca acaaatggtg
     2041 gaaacaaaaa tct
//
LOCUS       DAZKKW010000344         1921 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.guided.208, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000344 DAZKKW010000000
VERSION     DAZKKW010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1921
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.guided.208"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            83..994
                     /gene="vanY"
                     /locus_tag="ACG8PO_003142"
     CDS             83..994
                     /gene="vanY"
                     /locus_tag="ACG8PO_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-A/VanY-F/VanY-M family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HFQ0208366.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYELWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
     gene            1147..1632
                     /gene="vanZ-A"
                     /locus_tag="ACG8PO_003143"
     CDS             1147..1632
                     /gene="vanZ-A"
                     /locus_tag="ACG8PO_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HFQ0208367.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
BASE COUNT          678 a          270 c          359 g          614 t
ORIGIN      
        1 taagatttct ctttaccaac atcatttgac aaagagccaa tatttcatac ttccaactat
       61 atagttaagg aggagactga aaatgaagaa gttgtttttt ttattgttat tgttattctt
      121 aatatactta ggttatgact acgttaatga agcactgttt tctcaggaaa aagtcgaatt
      181 tcaaaattat gatcaaaatc ccaaagaaca tttagaaaat agtgggactt ctgaaaatac
      241 ccaagagaaa acaattacag aagaacaggt ttatcaagga aatctgctat taatcaatag
      301 taaatatcct gttcgccaag aaagtgtgaa gtcagatatc gtgaatttat ctaaacatga
      361 cgaattaata aatggatacg ggttgcttga tagtaatatt tatatgtcaa aagaaatagc
      421 acaaaaattt tcagagatgg tcaatgatgc tgtaaagggt ggcgttagtc attttattat
      481 taatagtggc tatcgagact ttgatgagca aagtgtgctt taccaagaaa tgggggctga
      541 gtatgcctta ccagcaggtt atagtgagca taattcaggt ttatcactag atgtaggatc
      601 aagcttgacg aaaatggaac gagcccctga aggaaagtgg atagaagaaa atgcttggaa
      661 atacgggttc attttacgtt atccagagga caaaacagag ttaacaggaa ttcaatatga
      721 actatggcat attcgctatg ttggtttacc acatagtgcg attatgaaag aaaagaattt
      781 cgttctcgag gaatatatgg attacctaaa agaagaaaaa accatttctg ttagtgtaaa
      841 tggggaaaaa tatgagatct tttattatcc tgttactaaa aataccacca ttcatgtgcc
      901 gactaatctt cgttatgaga tatcaggaaa caatatagac ggtgtaattg tgacagtgtt
      961 tcccggatca acacatacta attcaaggag gtaaggatgg cggaatgaaa ccaacgaaat
     1021 taatgaacag cattattgta ctagcacttt tggggtaacg ttagcttttt aatttaaaac
     1081 ccacgttaac taggacattg ctatactaat gatacaactt aaacaaaaga attagaggaa
     1141 attatattgg gaaaaatatt atctagagga ttgctagctt tatatttagt gacactaatc
     1201 tggttagtgt tattcaaatt acaatacaat attttatcag tatttaatta tcatcaaaga
     1261 agtcttaact tgactccatt tactgctact gggaatttca gagagatgat agataatgtt
     1321 ataatcttta ttccatttgg cttgcttttg aatgtcaatt ttaaagaaat cggattttta
     1381 cctaagtttg cttttgtact ggttttaagt cttacttttg aaataattca atttatcttc
     1441 gctattggag cgacagacat aacagatgta attacaaata ctgttggagg ctttcttgga
     1501 ctgaaattat atggtttaag caataagcat atgaatcaaa aaaaattaga cagagttatt
     1561 atttttgtag gtatactttt gctcgtatta ttgctcgttt accgtaccca tttaagaata
     1621 aattacgtgt aagatgtcta aatcaagcaa tctgatcttt catacacata aagatattga
     1681 atgaattgga ttagatggaa aacgggatgt ggggaaactc gcccgtaggt gtgaagtgag
     1741 gggaaaaccg gtgataaagt aaaaagctta cctaacacta tagtaacaaa gaaagcccaa
     1801 ttatcaattt tagtgctgag gaattggtct ctttaataaa tttccttaac gttgtaaatc
     1861 cgcattttcc tgacggtacc cccaaactaa gatttcatag atagattttc tgataacttt
     1921 a
//
LOCUS       DAZKKW010000345         1781 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.guided.216, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000345 DAZKKW010000000
VERSION     DAZKKW010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1781
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.guided.216"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            24..416
                     /locus_tag="ACG8PO_003144"
     CDS             24..416
                     /locus_tag="ACG8PO_003144"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0208368.1"
                     /translation="MKLLFIDDNMVFDIDNLKGFLNDTSSFGFIAKENNKIIGFAYCY
                     TLLRPDGKTMFYLHSIGMLPNYQDKGYGSKLLSFIKEYSKEIGCSEMFLITDKGNPRA
                     CHVYEKLGGKNDYKDEIVYVYDYEKGDK"
     gene            417..>1781
                     /gene="aph(2'')-I"
                     /locus_tag="ACG8PO_003145"
     CDS             417..>1781
                     /gene="aph(2'')-I"
                     /locus_tag="ACG8PO_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APH(2'')-Ia/If/Ih family aminoglycoside
                     O-phosphotransferase"
                     /protein_id="HFQ0208369.1"
                     /translation="MNIVENEICIRTLIDDDFPLMLKWLTDERVLEFYDGRDKKYTLE
                     SLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHYPKTDEIVYGMDQFI
                     GEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIRAYQKSGFRIIEDLP
                     EHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDSIEIIGSGYDSVAYL
                     VNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIEYSYISDELSILGYKE
                     IKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISECTIDNKQNVLEEYIL
                     LRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSCNHLLLDGNNRLTGII
                     DFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIEKAKEYQDIVEEYYPI
                     ETIVY"
BASE COUNT          731 a          136 c          303 g          611 t
ORIGIN      
        1 tatagttgaa gaatattatc ctattgaaac tattgtttat tgatgataat atggtttttg
       61 atattgataa tttaaaagga tttcttaatg ataccagttc atttgggttt atagctaaag
      121 aaaataataa aattatagga tttgcatatt gctatacact tttaagacct gatggaaaaa
      181 caatgtttta tttacactca ataggaatgt tacctaacta tcaagacaaa ggttatggtt
      241 caaaattatt atcttttatt aaggaatatt ctaaagagat tggttgttct gaaatgtttt
      301 taataactga taaaggtaat cctagagctt gccatgtata tgaaaaatta ggtggtaaaa
      361 atgattataa agatgaaata gtatatgtat atgattatga aaaaggtgat aaataaatga
      421 atatagttga aaatgaaata tgtataagaa ctttaataga tgatgatttt cctttgatgt
      481 taaaatggtt aactgatgaa agagtattag aattttatga tggtagagat aaaaaatata
      541 cattagaatc attaaaaaaa cattatacag agccttggga agatgaagtt tttagagtaa
      601 ttattgaata taacaatgtt cctattggat atggacaaat atataaaatg tatgatgagt
      661 tatatactga ttatcattat ccaaaaactg atgagatagt ctatggtatg gatcaattta
      721 taggagagcc aaattattgg agtaaaggaa ttggtacaag atatattaaa ttgatttttg
      781 aatttttgaa aaaagaaaga aatgctaatg cagttatttt agaccctcat aaaaataatc
      841 caagagcaat aagggcatac caaaaatctg gttttagaat tattgaagat ttgccagaac
      901 atgaattaca cgagggcaaa aaagaagatt gttatttaat ggaatataga tatgatgata
      961 atgccacaaa tgttaaggca atgaaatatt taattgagca ttactttgat aatttcaaag
     1021 tagatagtat tgaaataatc ggtagtggtt atgatagtgt ggcatattta gttaataatg
     1081 aatacatttt taaaacaaaa tttagtacta ataagaaaaa aggttatgca aaagaaaaag
     1141 caatatataa ttttttaaat acaaatttag aaactaatgt aaaaattcct aatattgaat
     1201 attcgtatat tagtgatgaa ttatctatac taggttataa agaaattaaa ggaacttttt
     1261 taacaccaga aatttattct actatgtcag aagaagaaca aaatttgtta aaacgagata
     1321 ttgccagttt tttaagacaa atgcacggtt tagattatac agatattagt gaatgtacta
     1381 ttgataataa acaaaatgta ttagaagagt atatattgtt gcgtgaaact atttataatg
     1441 atttaactga tatagaaaaa gattatatag aaagttttat ggaaagacta aatgcaacaa
     1501 cagtttttga gggtaaaaag tgtttatgcc ataatgattt tagttgtaat catctattgt
     1561 tagatggcaa taatagatta actggaataa ttgattttgg agattctgga attatagatg
     1621 aatattgtga ttttatatac ttacttgaag atagtgaaga agaaatagga acaaattttg
     1681 gagaagatat attaagaatg tatggaaata tagatattga gaaagcaaaa gaatatcaag
     1741 atatagttga agaatattat cctattgaaa ctattgttta t
//
LOCUS       DAZKKW010000346         1390 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.guided.236, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000346 DAZKKW010000000
VERSION     DAZKKW010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1390
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.guided.236"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1311
                     /locus_tag="ACG8PO_003146"
     CDS             <1..1311
                     /locus_tag="ACG8PO_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aminoglycoside N-acetyltransferase
                     AAC(6')-Ie/aminoglycoside O-phosphotransferase
                     APH(2'')-Ia"
                     /protein_id="HFQ0208370.1"
                     /translation="ESLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYH
                     YPKTDEIVYGMDQFIGEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAI
                     RAYQKSGFRIIEDLPEHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVD
                     SIEIIGSGYDSVAYLVNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIE
                     YSYISDELSILGYKEIKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISE
                     CTIDNKQNVLEEYILLRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSC
                     NHLLLDGNNRLTGIIDFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIE
                     KAKEYQDIVEEYYPIETIVYGIKNIKQEFIENGRKEIYKRTYKD"
BASE COUNT          597 a          103 c          235 g          455 t
ORIGIN      
        1 gaatcattaa aaaaacatta tacagagcct tgggaagatg aagtttttag agtaattatt
       61 gaatataaca atgttcctat tggatatgga caaatatata aaatgtatga tgagttatat
      121 actgattatc attatccaaa aactgatgag atagtctatg gtatggatca atttatagga
      181 gagccaaatt attggagtaa aggaattggt acaagatata ttaaattgat ttttgaattt
      241 ttgaaaaaag aaagaaatgc taatgcagtt attttagacc ctcataaaaa taatccaaga
      301 gcaataaggg cataccaaaa atctggtttt agaattattg aagatttgcc agaacatgaa
      361 ttacacgagg gcaaaaaaga agattgttat ttaatggaat atagatatga tgataatgcc
      421 acaaatgtta aggcaatgaa atatttaatt gagcattact ttgataattt caaagtagat
      481 agtattgaaa taatcggtag tggttatgat agtgtggcat atttagttaa taatgaatac
      541 atttttaaaa caaaatttag tactaataag aaaaaaggtt atgcaaaaga aaaagcaata
      601 tataattttt taaatacaaa tttagaaact aatgtaaaaa ttcctaatat tgaatattcg
      661 tatattagtg atgaattatc tatactaggt tataaagaaa ttaaaggaac ttttttaaca
      721 ccagaaattt attctactat gtcagaagaa gaacaaaatt tgttaaaacg agatattgcc
      781 agttttttaa gacaaatgca cggtttagat tatacagata ttagtgaatg tactattgat
      841 aataaacaaa atgtattaga agagtatata ttgttgcgtg aaactattta taatgattta
      901 actgatatag aaaaagatta tatagaaagt tttatggaaa gactaaatgc aacaacagtt
      961 tttgagggta aaaagtgttt atgccataat gattttagtt gtaatcatct attgttagat
     1021 ggcaataata gattaactgg aataattgat tttggagatt ctggaattat agatgaatat
     1081 tgtgatttta tatacttact tgaagatagt gaagaagaaa taggaacaaa ttttggagaa
     1141 gatatattaa gaatgtatgg aaatatagat attgagaaag caaaagaata tcaagatata
     1201 gttgaagaat attatcctat tgaaactatt gtttatggaa ttaaaaatat taaacaggaa
     1261 tttatcgaaa atggtagaaa agaaatttat aaaaggactt ataaagattg attatataat
     1321 atatgaaaag ctattataaa agacattagt attaaatagt ttaaaaaaat gaaaaataat
     1381 aaaggaagtg
//
LOCUS       DAZKKW010000347          909 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3901
            SAMEA115706963-rid22575973.guided.285, whole genome shotgun
            sequence.
ACCESSION   DAZKKW010000347 DAZKKW010000000
VERSION     DAZKKW010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706963
            Sequence Read Archive: ERR13196697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287839.1
            Genome Coverage       :: 148x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,147
            CDSs (total)                      :: 3,084
            Genes (coding)                    :: 3,009
            CDSs (with protein)               :: 3,009
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 24 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..909
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706963-rid22575973.guided.285"
                     /isolate="VRE3901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            283..831
                     /gene="aac(6')"
                     /locus_tag="ACG8PO_003147"
     CDS             283..831
                     /gene="aac(6')"
                     /locus_tag="ACG8PO_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HFQ0208371.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          322 a          130 c          188 g          269 t
ORIGIN      
        1 attatatgaa aacaggtttt atttgtcaat tcataattgg ttttttttaa agcaaaagat
       61 aatctatatc atgatgaaca aacagagttt tttcctccaa ttgatggctt tcctgatctt
      121 tcctattttg aataaagtgt ttctgtataa tttttttatt ttataagtaa gataaataat
      181 gaaagaatag agatactgat tggtagctaa agatttttga gcgagaacat ttcgtttttt
      241 caaaaggact gttagaataa gcaagaacaa gggggaagac gtatgataat cagtgaattt
      301 gaccgtaata atccagtatt gaaagatcag ctttctgatt tactgagact gacttggccg
      361 gaagaatatg gagacagctc ggcagaagaa gtagaagaaa tgatgaatcc agaacgaatc
      421 gcggtagcag cggtagacca agatgagtta gtaggattta ttggtgcaat ccctcaatac
      481 ggtatcacag gttgggaatt gcatccatta gttgtagaaa gctcccgacg aaagaaccaa
      541 ataggtactc gattagtcaa ttacttagaa aaagaagtag cttccagagg aggaatcacg
      601 atttatttag gtacggatga tttagaccat ggaacaacgt taagtcaaac ggacctgtat
      661 gagcatacat ttgataaagt ggcttccatc cagaaccttc gtgaacatcc gtatgaattc
      721 tatgaaaaat taggttataa aatcgtaggt gtcttaccaa atgcaaatgg ctgggacaaa
      781 ccggatattt ggatggcaaa aacgattatt cctcgaccaa attctcaata ataaaaagag
      841 gttgtgacag aagtaatcag cttcaagaaa taagaaggaa tttacgaaaa ttacttcttg
      901 aatttttgt
//
